LOCUS       DAVKGU010000001        71703 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKGU010000001 DAVKGU010000000
VERSION     DAVKGU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 71703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.001"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            635..1762
                     /locus_tag="VW077_000001"
     CDS             635..1762
                     /locus_tag="VW077_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9454103.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWCYLNSNGS
                     MKVNQWFQVGGKWYYVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(2423..3451)
                     /gene="queA"
                     /locus_tag="VW077_000002"
     CDS             complement(2423..3451)
                     /gene="queA"
                     /locus_tag="VW077_000002"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9454104.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            3602..4309
                     /locus_tag="VW077_000003"
     CDS             3602..4309
                     /locus_tag="VW077_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9454105.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            4449..4625
                     /locus_tag="VW077_000004"
     CDS             4449..4625
                     /locus_tag="VW077_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9454106.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            4755..5690
                     /locus_tag="VW077_000005"
     CDS             4755..5690
                     /locus_tag="VW077_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9454107.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPKLLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            5683..6471
                     /locus_tag="VW077_000006"
     CDS             5683..6471
                     /locus_tag="VW077_000006"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9454108.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            6472..6855
                     /locus_tag="VW077_000007"
     CDS             6472..6855
                     /locus_tag="VW077_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9454109.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            6871..7260
                     /locus_tag="VW077_000008"
     CDS             6871..7260
                     /locus_tag="VW077_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9454110.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYALQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            7344..8474
                     /locus_tag="VW077_000009"
     CDS             7344..8474
                     /locus_tag="VW077_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9454111.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            8479..9216
                     /locus_tag="VW077_000010"
     CDS             8479..9216
                     /locus_tag="VW077_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9454112.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWKEIKKA"
     gene            9227..10000
                     /locus_tag="VW077_000011"
     CDS             9227..10000
                     /locus_tag="VW077_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9454113.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            9990..10607
                     /locus_tag="VW077_000012"
     CDS             9990..10607
                     /locus_tag="VW077_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9454114.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            10817..11218
                     /locus_tag="VW077_000013"
     CDS             10817..11218
                     /locus_tag="VW077_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9454115.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            11218..11496
                     /locus_tag="VW077_000014"
     CDS             11218..11496
                     /locus_tag="VW077_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9454116.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            11486..12259
                     /locus_tag="VW077_000015"
     CDS             11486..12259
                     /locus_tag="VW077_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9454117.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            12260..13564
                     /locus_tag="VW077_000016"
     CDS             12260..13564
                     /locus_tag="VW077_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9454118.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            14138..15016
                     /gene="pstS"
                     /locus_tag="VW077_000017"
     CDS             14138..15016
                     /gene="pstS"
                     /locus_tag="VW077_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9454119.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            15016..15933
                     /gene="pstC"
                     /locus_tag="VW077_000018"
     CDS             15016..15933
                     /gene="pstC"
                     /locus_tag="VW077_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9454120.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            15923..16807
                     /gene="pstA"
                     /locus_tag="VW077_000019"
     CDS             15923..16807
                     /gene="pstA"
                     /locus_tag="VW077_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9454121.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            16818..17621
                     /gene="pstB"
                     /locus_tag="VW077_000020"
     CDS             16818..17621
                     /gene="pstB"
                     /locus_tag="VW077_000020"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9454122.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            17634..18392
                     /gene="pstB"
                     /locus_tag="VW077_000021"
     CDS             17634..18392
                     /gene="pstB"
                     /locus_tag="VW077_000021"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9454123.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            18404..19057
                     /gene="phoU"
                     /locus_tag="VW077_000022"
     CDS             18404..19057
                     /gene="phoU"
                     /locus_tag="VW077_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9454124.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            19214..20029
                     /locus_tag="VW077_000023"
     CDS             19214..20029
                     /locus_tag="VW077_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9454125.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(20049..21320)
                     /locus_tag="VW077_000024"
     CDS             complement(20049..21320)
                     /locus_tag="VW077_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9454126.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFDQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRAILDLAAKYSVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNTIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            21462..22193
                     /gene="budA"
                     /locus_tag="VW077_000025"
     CDS             21462..22193
                     /gene="budA"
                     /locus_tag="VW077_000025"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9454127.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKESGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADIATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            22203..22955
                     /locus_tag="VW077_000026"
     CDS             22203..22955
                     /locus_tag="VW077_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9454128.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            23067..23972
                     /gene="murB"
                     /locus_tag="VW077_000027"
     CDS             23067..23972
                     /gene="murB"
                     /locus_tag="VW077_000027"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9454129.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            24132..25289
                     /locus_tag="VW077_000028"
     CDS             24132..25289
                     /locus_tag="VW077_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454130.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            25270..26076
                     /locus_tag="VW077_000029"
     CDS             25270..26076
                     /locus_tag="VW077_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454131.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WLNLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            26073..26846
                     /locus_tag="VW077_000030"
     CDS             26073..26846
                     /locus_tag="VW077_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454132.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            26843..27913
                     /locus_tag="VW077_000031"
     CDS             26843..27913
                     /locus_tag="VW077_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9454133.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            28174..28413
                     /locus_tag="VW077_000032"
     CDS             28174..28413
                     /locus_tag="VW077_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454134.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            28688..29173
                     /locus_tag="VW077_000033"
     CDS             28688..29173
                     /locus_tag="VW077_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9454135.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            29195..31813
                     /gene="alaS"
                     /locus_tag="VW077_000034"
     CDS             29195..31813
                     /gene="alaS"
                     /locus_tag="VW077_000034"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9454136.1"
                     /translation="MKQLSSAQVRQMWLDFWATKAHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDSSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(31903..33357)
                     /locus_tag="VW077_000035"
     CDS             complement(31903..33357)
                     /locus_tag="VW077_000035"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9454137.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPIADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            33536..34285
                     /locus_tag="VW077_000036"
     CDS             33536..34285
                     /locus_tag="VW077_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454138.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQDLSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSSSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDLDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRRDNPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            34266..35900
                     /locus_tag="VW077_000037"
     CDS             34266..35900
                     /locus_tag="VW077_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454139.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNTGKKVNVSARV
                     LSSYIFSSLLMLIFFSTIAIHFPFEEMPVYFSFVVAILLVIAFSTSLTAFYNVFYESK
                     DLASYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTTAFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPILTLLGLSLYLGLPIISMIYLIMAWILASVIL
                     SCHHYFKDVKNLSINWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            36585..37748
                     /locus_tag="VW077_000038"
     CDS             36585..37748
                     /locus_tag="VW077_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9454140.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVTFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLTMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            37745..38422
                     /locus_tag="VW077_000039"
     CDS             37745..38422
                     /locus_tag="VW077_000039"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9454141.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            38412..39266
                     /locus_tag="VW077_000040"
     CDS             38412..39266
                     /locus_tag="VW077_000040"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9454142.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATDTNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            39285..40352
                     /locus_tag="VW077_000041"
     CDS             39285..40352
                     /locus_tag="VW077_000041"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9454143.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            40362..41528
                     /gene="aroC"
                     /locus_tag="VW077_000042"
     CDS             40362..41528
                     /gene="aroC"
                     /locus_tag="VW077_000042"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9454144.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            41538..42641
                     /locus_tag="VW077_000043"
     CDS             41538..42641
                     /locus_tag="VW077_000043"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9454145.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            42652..42990
                     /locus_tag="VW077_000044"
     CDS             42652..42990
                     /locus_tag="VW077_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9454146.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            43082..44365
                     /gene="aroA"
                     /locus_tag="VW077_000045"
     CDS             43082..44365
                     /gene="aroA"
                     /locus_tag="VW077_000045"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9454147.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQDVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVAGGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            44358..44834
                     /locus_tag="VW077_000046"
     CDS             44358..44834
                     /locus_tag="VW077_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9454148.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            44831..45679
                     /gene="pheA"
                     /locus_tag="VW077_000047"
     CDS             44831..45679
                     /gene="pheA"
                     /locus_tag="VW077_000047"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9454149.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            45676..46950
                     /gene="psr"
                     /locus_tag="VW077_000048"
     CDS             45676..46950
                     /gene="psr"
                     /locus_tag="VW077_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9454150.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            47229..48074
                     /locus_tag="VW077_000049"
     CDS             47229..48074
                     /locus_tag="VW077_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9454151.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSCILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            48064..49167
                     /locus_tag="VW077_000050"
     CDS             48064..49167
                     /locus_tag="VW077_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9454152.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLYQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            49169..50155
                     /locus_tag="VW077_000051"
     CDS             49169..50155
                     /locus_tag="VW077_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9454153.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATDLSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNSIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            50171..51604
                     /locus_tag="VW077_000052"
     CDS             50171..51604
                     /locus_tag="VW077_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9454154.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQAEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGILFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            51633..52667
                     /locus_tag="VW077_000053"
     CDS             51633..52667
                     /locus_tag="VW077_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9454155.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            52867..53604
                     /gene="mecA"
                     /locus_tag="VW077_000054"
     CDS             52867..53604
                     /gene="mecA"
                     /locus_tag="VW077_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9454156.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            53755..55041
                     /locus_tag="VW077_000055"
     CDS             53755..55041
                     /locus_tag="VW077_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9454157.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQEFGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            55043..55912
                     /locus_tag="VW077_000056"
     CDS             55043..55912
                     /locus_tag="VW077_000056"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9454158.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            55988..56062
                     /locus_tag="VW077_000057"
                     /pseudo
     CDS             55988..56062
                     /locus_tag="VW077_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            56207..57145
                     /gene="msrB"
                     /locus_tag="VW077_000058"
     CDS             56207..57145
                     /gene="msrB"
                     /locus_tag="VW077_000058"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9454159.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            57203..58927
                     /locus_tag="VW077_000059"
     CDS             57203..58927
                     /locus_tag="VW077_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454160.1"
                     /translation="MKYLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLIQLILGLYPVNKGRIDL
                     YLDGLSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIREDLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            58929..60677
                     /locus_tag="VW077_000060"
     CDS             58929..60677
                     /locus_tag="VW077_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454161.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFFLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNLEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            61024..62283
                     /locus_tag="VW077_000061"
     CDS             61024..62283
                     /locus_tag="VW077_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9454162.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPSGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKALGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSNDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    62359..62494
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            62533..63033
                     /locus_tag="VW077_000062"
     CDS             62533..63033
                     /locus_tag="VW077_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9454163.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            63109..63477
                     /gene="rplL"
                     /locus_tag="VW077_000063"
     CDS             63109..63477
                     /gene="rplL"
                     /locus_tag="VW077_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9454164.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            63679..63852
                     /locus_tag="VW077_000064"
     CDS             63679..63852
                     /locus_tag="VW077_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454165.1"
                     /translation="MTKELQSSRYIVISFLVREMGIDIVEAISLMAELEKSGLVRLES
                     SGDLILKELGGAL"
     gene            63849..64628
                     /locus_tag="VW077_000065"
     CDS             63849..64628
                     /locus_tag="VW077_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9454166.1"
                     /translation="MKRITANQYQTSERYYKLPKILFEDEKYMDMKLEVKVAYSILKD
                     RLELSLSRGWIDEEGAVYLVFSNSKLMKLLGCSKSKLLSIKKILKEYDLIDEVQQSSS
                     EKGRLANKIYLGELSSTPVASSNRPSVKKKIGQVENETAPVSHSAPSETEVSETKYSE
                     TDSLFSEDEEERYTQPILKRKVEKVTKYDQDYIWGLVQDQFRREGFSETASEIAMTDF
                     ERIYQYALDNVRFVRRAEVLAEFVFNGLYSVWNNRVRKGGG"
     gene            64727..66085
                     /gene="dcm"
                     /locus_tag="VW077_000066"
     CDS             64727..66085
                     /gene="dcm"
                     /locus_tag="VW077_000066"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU9454167.1"
                     /translation="MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQT
                     EGEIEFHDIRDVSDDEFKKLRGKVDIICGGFPCQAFSIAGRRLGFEDIRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
                     RVFIIGHSRKKGIRLLFPFRREGQATNSETLKTLGNLNPSKSGMSGKVYYSEGLAPTL
                     VRGKGEGFKIAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFK
                     ETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLE
                     RPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGILIDGQFYRLRIRR
                     ITPKECFRLQGFPDWAFEAARRVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDES
                     IK"
     gene            66069..66518
                     /locus_tag="VW077_000067"
     CDS             66069..66518
                     /locus_tag="VW077_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454168.1"
                     /translation="MKALNKESILDCDELETELHDAEIKQLDEQLFLIPNYPCEFEVT
                     FLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDHQNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKKPINFSNLLDSLIVSEYQMEPNLWEVSHD"
     gene            66511..66891
                     /locus_tag="VW077_000068"
     CDS             66511..66891
                     /locus_tag="VW077_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9454169.1"
                     /translation="MIDLYLSKNCHRNQLLLDFFQKYGIEVTPHSISEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLKLTDFIEMILKNPELTIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            66904..67137
                     /locus_tag="VW077_000069"
     CDS             66904..67137
                     /locus_tag="VW077_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9454170.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            67140..67727
                     /locus_tag="VW077_000070"
     CDS             67140..67727
                     /locus_tag="VW077_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9454171.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKLLCISELTILQILYCASLIS
                     FLWMYGQRKQVVKVNMKTRMKWLGIGFVSLLIINLCFSLIHAQGTTNQANLIGLQHQV
                     PWFSFLLLLINASMVEEFLYREILWNLVRKLDIRVALTSILFVLAHHPGTILAWCLYV
                     SLGMFLGLVRYKSDLWGSMGLHLVWNLSVYVLFFL"
     gene            complement(67942..69158)
                     /locus_tag="VW077_000071"
                     /pseudo
     CDS             complement(67942..69158)
                     /locus_tag="VW077_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(69240..69443)
                     /locus_tag="VW077_000072"
     CDS             complement(69240..69443)
                     /locus_tag="VW077_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEU9454172.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            complement(69904..70134)
                     /locus_tag="VW077_000073"
     CDS             complement(69904..70134)
                     /locus_tag="VW077_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9454173.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(70131..70553)
                     /locus_tag="VW077_000074"
     CDS             complement(70131..70553)
                     /locus_tag="VW077_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9454174.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            71058..71411
                     /locus_tag="VW077_000075"
     CDS             71058..71411
                     /locus_tag="VW077_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9454175.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(71471..71638)
                     /locus_tag="VW077_000076"
     CDS             complement(71471..71638)
                     /locus_tag="VW077_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEU9454176.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
BASE COUNT        20945 a        13316 c        15926 g        21516 t
ORIGIN      
        1 agtacaagac agatgttcta gttccttctt taatctggtt tagtattagt taaaaaatcg
       61 ctttaagctt gtaactaaga gggagctaat cgactagatt ctccagccga acaggtggta
      121 atgtactttt tatagtgtaa tcctagctgt tgttaaattt aaaatagaat cctctatcga
      181 gttagggaat taaattcaac caattttatt catgtttttt ctatcaaaat atctaatatt
      241 aaaatagtct cattctgatg agaaaactat tctatttata ctgcatagtt gtataaggaa
      301 tgatgaaaga tgagtgaaag ttatcaagtt ggaatgtttg tatctaaata tattagcatg
      361 tatttagata agatgtctgc aatcctttat atatgagatt gattacgata tagttaaagc
      421 gttagttcaa taatcaatat atcattctaa gtatatttga tatataaagt atactgttta
      481 acgtttgata gaaaagatgc tatactagaa gtagtaactt ttagaagtag gatgtaatgg
      541 caaaatctaa taccaataag cgctatgcat tacgtaaact cggaaagatt tttggttctg
      601 cggtaatcgg gctaacttta tttttaggag gaatctgaat acaagttttg ttcatgctgc
      661 tgatgggatt caatatgtca gagatgatac tagagataaa gaagagggaa tagagtatga
      721 tgacgctgac aatggggata ttattgtaaa agtagcgact aaacctaagg tagtaaccaa
      781 gaaaatttca agtacgcgaa ttcgttatga aaaagatgaa acaaaagacc gtagtgaaaa
      841 tcctgttaca attgatggag aggatggcta tgtaactacg acaaggacct acgatgttaa
      901 tccagagact ggtcatgtta ccgaacaggt tactgttgat agaaaagaag ccacggatac
      961 agttatcaaa gttccagcta aaagcaaggt tgaagaagtt cttgttccat gtgctactaa
     1021 atatgaagca gacaatgacc tttctgcagg acaggagcaa gagattactc taggaaagaa
     1081 tgggaaaaca gttacaacga taacttatga tgtagatgga aagagtggac aagtaactga
     1141 gagtacttta agtcaaaaag aagactctca aacaagagtt gttaaaaaag gaaccaagcc
     1201 ccaagttctt gtccaagaaa ttccaatcga aacagaatat ctcgatgacc caactcttga
     1261 taaaggtcaa gaagtaggag aaattggtaa aattactctt actacaatct atgctgtaga
     1321 tgaacgtgat ggaacaattg aagaaactac ttctcgtcaa attactaaag agatggtaaa
     1381 aagacgtata aggagaggga cgagagaacc tgaaaaagtt gttgttcctg agcaatcatc
     1441 tattccttcg tatcctgtat ctgttacatc taaccaagga acagatgtag cagtagaacc
     1501 agctaaagca gttgctccaa caacaggctg gaaacaagaa aatggtatgt ggtattttta
     1561 taatactgat ggttccatgg caacaggttg ggtacaagtt aatggttcat ggtgctacct
     1621 caacagcaac ggttctatga aagttaatca atggttccaa gttggtggta aatggtatta
     1681 tgtaaatgca tcgggtgagt tagcggtcaa tacaagtata gatggctata gagtcaatga
     1741 taatggtgaa tgggtgcgtt aacagattcc tccctgagac aaaattgata atagtatgtc
     1801 tgaatatata attatatata aaaagctata gataaggtca tcttatagcg actataaaaa
     1861 agtgaacaag cctagtcttc tgtacattag aattcagctt tgttcacttt ttgtttaata
     1921 ctcaatgaaa atcaaagagc aaactagaaa actagtcgca ggttgctcaa agtactgctt
     1981 tgaggttgta gatagaactg acaaagtcag tcacatatat aatccaaggt gacgttgacg
     2041 cggtttgaag agatttttga agagtataag tctttgattt tgagactaag gagagtcagg
     2101 gtcaaagtag tgaatgattc tgaaataact cataatagtc tcttgagagg acagataaga
     2161 gtaacaaata tggtgctact catgtgtctt gcattttgat ttacaaacag cgaagaaagt
     2221 ataggaagct gaaataaaat atagatgaat caatttggag agtcaacgca atctatggca
     2281 atattttcga agctaggggg aagcatttta atttcaatgt actagacctc ttctacagct
     2341 agctagagtt tcaattgtag atgttctttg aaataatatc ttagcgattt attggtatta
     2401 gaagatttag agaaattctt tctcaataaa taaacatggc gtcaccaaaa ctgaagaagc
     2461 ggtagtgttc ttggatggaa tggtggtagg catctaagac taattcacgg cctgcaaagg
     2521 cagaaaccaa catgaccaga gttgattttg gcaggtggaa gttggttgag aaggcatcca
     2581 cgaccttcca ctcataccca ggtttgataa agatattggt ccaaccagaa tctgcttgga
     2641 tttgcccatc aaacttggaa ccaatagttt ccaaggtgcg gatagaagtg gttccgacag
     2701 cgatgacacg accaccattt tttttgacag agcgaagggt ggcagcagct tcctcagaaa
     2761 gttgatagaa ctctgagtgc atttcgtgtt cgtccagatt atccacagaa acaggtctaa
     2821 aggttccgag tccgacatgg agagtcagat agactagatg aacaccctta gcttggattt
     2881 ctgccagcag ttctttggtg aagtgaagac cagcagtcgg tgctgcagca gagccgcttt
     2941 ccttggcgta gacggtttga taacgttcac ggtcatctaa tttttcgtgg atataaggtg
     3001 gcagaggcat ttctcccaga ctttccaaga cttctaggaa aattccttgg tattcaaagc
     3061 ggacaatgcg tcccccgtgg gtcaattctt ctgtaacgac agcgctgagg cggccatcac
     3121 caaagctgat acgagtaccg accttgaggc gtttggcagg tttagccaga acttcccact
     3181 cgtctccact agtgttctta aggaggagaa gttccacatg acctcctgtc tccacttttt
     3241 gaccatagag gcgggcaggg agaactcggg tgtcgttcat gacaagggca tcaccaggtt
     3301 ccagcatatt aataatagag tggaagtgtt tatcttgcat ttctcctgtc tcacggttga
     3361 cgatgaggag tttggaggca tcacgttttt caaggggcgt ttgggcaatc aattcctctg
     3421 gcaagtggaa atcaaaatca gctgtattca tatatctgtt cattctttct aagttctaat
     3481 cctatccatt ataacacgtt tcaagtttgg acgctctttt tttagaaatt aaatcgtttt
     3541 cacttgacaa aaattggtct ataccatata ataaatatag acagaaatgg aggatgaaaa
     3601 gatgaaagtt attaaagttg aaaaccaagt tcaaggtgga aaagttgctt ttgagatttt
     3661 gaaggaaaaa ttggctaatg gagctcaaac tctaggactt gcgacaggaa gtagcccact
     3721 tgaattttac aaggaaattg ttgagagtga ccttgatttt tcaaatctaa ccagtgtcaa
     3781 ccttgatgag tatgtgggcc ttgatgggga caatccacag tcttatcgtt acttcatgca
     3841 agaaaacttg ttcaaccaaa aaccatttaa agaaagtttc ttgcctcgtg gtgttaagga
     3901 caatgctgaa gctgaagttg aacgctacaa ccaaattttg gctgaccatc cagttgacct
     3961 ccaaatcttg ggaatcggtc gcaatgggca tatcggcttt aatgagcctg gtactccatt
     4021 tgacagtcaa acgcatctag tagaacttga ccagtctact atcgaagcca atgcacgctt
     4081 ctttgccaag attgaagacg tcccaaccca agctatttca atggggatta aaaacatttt
     4141 ggatgccaag tctattattc tttttgctta cggtgagtcg aaagcagaag ccattgccgg
     4201 aacagtatct ggcccagtga ctgagaacct acctgcaagt agcctccaaa atcaccctga
     4261 tgtaaccatc atcgcagatg ctgaagcgct tagcttactc gaaaaataaa aaagcaatgt
     4321 tctattgaac gctttcaact cttgtataaa tggaagaaaa aatcaaaatt aaaccgcatt
     4381 tttgcttgac aattattcct tttacgtgta gaatgaaata gatattgaac ttgaagggag
     4441 tgaaaaaaat gtctaaaaca gtagtacgta agaatgaatc tcttgacgac gcacttcgcc
     4501 gtttcaaacg tgcggttact aaagctggta ctcttcaaga aacacgcaaa cgtgaattct
     4561 atgaaaaacc ttctgtaaaa cgtaaacgta aatcagaagt agctcgtaaa cgtaaaaaat
     4621 tctaattgga atgaaaggct agagaaatct agtccttttt cttttaaata aatactccaa
     4681 agcctgcaaa aatctgaaac ttcctcctac aatttgatat aatagagaga agaactcatt
     4741 tgaaggagga aatgatgtcg gttttagtaa aagaagtgat tgaaaagctt agactagaca
     4801 ttgtctatgg tgaaccaaaa ttgcttgaaa aggaaatcaa tacagcggat attacgcgac
     4861 ctggtcttga aatgacaggc tattttgact actatacacc agagcggatt caacttttgg
     4921 ggatgaagga gtggtcttat ctgatcagca tgccttccaa cagccgttat gaagttttga
     4981 aaaaaatgtt tctacctgag acaccagcag tcattgttgc ccgtggtttg gtggttccag
     5041 aggagatgtt aaaggctgct agagaatgta agattgctat tttaaccagc cgtgcagcta
     5101 ccagtcgttt atctggagag ttatctagct atctggattc tcgtttggca gaacgtacca
     5161 gtgtgcacgg tgtcttgatg gatatttatg ggatgggcgt cttgattcag ggagatagtg
     5221 gaattggtaa gagcgagaca ggtcttgagc ttgtcaaacg tggtcaccgt ttggtagccg
     5281 atgaccgtgt cgatatcttt gccaaggatg agattactct ctggggtgaa ccagctgaaa
     5341 ttttgaaaca cttgattgaa attcgtgggg ttggtattat cgatgttatg agtctctacg
     5401 gtgcgagtgc tgtcaaggat tcttcacagg ttcagcttgc tgtctatttg gaaaattacg
     5461 atacgcataa gacctttgat cgtcttggaa acaatgcaga ggaacttgaa gtttctggcg
     5521 tagccattcc tcgtattcgt attccagtta aaacaggtcg taatatctct gttgtgattg
     5581 aggcagctgc catgaattat cgtgccaagg aaatgggctt tgatgcgacc cgtttgttcg
     5641 acgaacgact gacaagtctc atcgctcgaa atgaggtgca aaatgcttga tccaattgct
     5701 attcaactag gacccctagc cattcgttgg tatgccttat gtattgtgac aggcttgatt
     5761 cttgcggttt atttgaccat gaaagaagca cctagaaaga agatcatacc agacgatatt
     5821 ttagatttta tcttagtagc ttttcccttg gctattttag gagctcgtct ctactatgtt
     5881 attttccgat ttgattacta tagtcagaat ttaggagaga tttttgccat ttggaatggt
     5941 ggtttggcca tttacggtgg tttgataact ggggctcttg tgctctatat ctttgctgac
     6001 cgtaaactca tcaatacttg ggattttcta gatattgcgg cgcctagcgt tatgattgct
     6061 caaagtttgg ggcgttgggg taatttcttt aaccaagaag cttatggtgc aacagtggat
     6121 aatctggatt atctacctgg ctttatccgt gaccagatgt atattgaggg gagctaccgt
     6181 caaccgactt tcctttatga gtctctatgg aatctgcttg gctttgcctt gattctgatt
     6241 tttagacgga aatggaagag tctcagacga ggtcatatca cggcctttta cttgatttgg
     6301 tatggtttcg gtcgtatggt catcgaaggt atgcgaacag atagtctcat gttcttcggc
     6361 cttcgagtgt cccaatggct gtcagttgtc cttatcggtc tcggtataat gatcgttatt
     6421 tatcaaaatc gaaagaaggc cccttactat attacagagg aggaaaacta aatgttagaa
     6481 gttgcatata ttcttgttgc cctagctttg attgtctttt tggtctatct gatcattact
     6541 gtacaaaagc ttggtcgtgt catcgatgaa acagaaaaga cgattaaaac cttgacttca
     6601 gatgtggatg tgaccttgca tcacaccaat gagttgttgg ctaaggtcaa tgtcttggca
     6661 gatgatatca atgtcaaggt ggctacgatt gatccactct tcagtgctgt tgcagattta
     6721 tctctatctg tttcagacct caatgaccat gcgcgtgtct tgagcaagaa agcttcatca
     6781 gctggttcaa aaacactcaa gactggtgca agtctgtcag ctcttcgtct tgcaagtaaa
     6841 tttttcaaaa aataaaaaag gagaatcctt atgggtaaat tatcctcaat ccttttagga
     6901 accgtttcag gtgcagctct tgccttgttt ttaacaagtg ataagggcaa acaagtttgc
     6961 agtcaggctc aagattttct agatgatttg agagaagatc cggagtatgc caaggagcaa
     7021 gtctgtgaaa aactgacaga agttaaggag caggctacag attttgttct gaaaacaaaa
     7081 gaacaggttg agtcaggtga aatcactgtg gacagtatac ttgctcaagc taaatcctat
     7141 gctcttcaag cgacagaagc atcaaaaaat caattaaata atctcaagga gcaatggcaa
     7201 gaaaaagccg aagctcttga tgactcagaa gagattgtga ttgatataac agaagaataa
     7261 accatcacca tctccggacg gactatgtat ctggggatgg tgatttttat ctggaatcta
     7321 gtctttgtgg tataataatt actatgcaga aaaaaccaac gtcagcctat gtgcacatcc
     7381 cattttgtac ccagatttgt tattattgtg atttttcaaa ggtcttcatc aaaaatcagc
     7441 cagtcgacag ctatttagag catctgctgg aagagtttcg ttcttatgat attgaaaagt
     7501 tgtcaaccct ttatatcggt ggtggaacac cgacagccct gtcggctccg caactggagg
     7561 ttttactgaa tggcttgact aaaaacttgg acttgtctgt cttggaagag ttgaccattg
     7621 aagccaatcc aggcgatttg gatgcggata agatagctgt tttgaaaaat tcggctgtca
     7681 atcgtgtttc gctaggtgtc cagacctttg atgataagat gttgaaaaag attgggcgca
     7741 gtcatttgga gaaggatatt tatgaaaata tcgatcgcct gaaactggct ggttttgaca
     7801 acatctccat tgatttgatt tatgctctgc ctggtcagac catggagcaa gtaaaggaaa
     7861 atgtggctaa agccattgga ctggatattc cccacatgag tttgtatagt ttgattttag
     7921 aaaaccatac ggtctttatg aaccggatgc gacgtgggaa attgcctctg cctaaggagg
     7981 aactagaagc ggagatgttt gagtacatca ttgcagagct ggagcgagcg ggttttgagc
     8041 attatgagat ttctaatttc tccaaacccg gctttgaaag tcgtcataat ctcatgtact
     8101 gggacaatgc tgaatactat ggtattggtg caggggcatc tggttatgtc aatggagtac
     8161 gttataaaaa tcatggtccc attcgtcatt atctcagtgc ggttgaggaa ggcaatgctt
     8221 gtattacaga agatcacctg agtcaaaagg agcaaatgga agaagaaatg ttcttgggac
     8281 tccgcaagaa atccggggtt tccatggcgc gatttgagga aaaatttgga cagtcttttg
     8341 ctggacttta tggagaaatt gtcagagatt tggttcaaca aggcctcatg cagattgagg
     8401 gtgaccacgt tcgcatgaca aagagaggtc tctttttggg agatactgta gcagaacgat
     8461 ttattttgga gtaggatcat gggcttaact tatcaaatga aaatgaaaat tccttttgat
     8521 atggctgata tgaacggtca tatcaaactt ccagatgtga ttttgctgtc actgcaagtt
     8581 tcagggatgc agtcgattga actgggagtt agtgataagg ccattttgga agactataat
     8641 ctggtctgga ttatcgcaga atatgatatt gaggtggttc gtttgcctcg ttttgcggaa
     8701 gaaattacca tcgaaacgga agccttgagc tacaatcgac ttttttgcta ccgtcgcttt
     8761 actatttata atgaagcggg gcaggagctc atccatatga tggcgacctt tgttctcatg
     8821 gaccgcgaca gtcgaaaagt ccatgctgtt gaacctgaga ttgtggctcc ttaccagtct
     8881 gattttgata aaaaacttat ccgtggaccg aagtatgagt ccttggaaga gccgatcagc
     8941 aaggattacc atgttcgttt ttacgacttg gatatgaatg gtcatgtcaa taacagtaaa
     9001 tacttggact ggatttttga ggtcatggga gcggattttt tgacccacta tattcccaag
     9061 aaaatcaacc tcaagtatgt caaggaagtt cgaccaggtg gggtgattac atcggctgtt
     9121 gaacggactg gactggaaag caagcatgaa attacaagtg atggggttat caatgcccaa
     9181 gcaatcatta cttggaaaga aataaaaaag gcttagggag aaatagatga aatataaagg
     9241 ctatttaatt gatttagacg gaaccattta taaggggaaa gaccgaatcc ctgctggaga
     9301 gacttttgtt catgaattgc aaaagcggga cattccctat ctctttgtaa ccaacaatac
     9361 aactcgcact ccagagagtg ttaaggagat gttggctcag aattttaata tcgatacgcc
     9421 cctatcgact gtctacacag cgactttggc aaccatcgac tatatgaatg atttggggct
     9481 tgaaaagacc gtctatgtcg tcggagaagc aggactcaag gaagccatca aggcggctgg
     9541 ttatgtggaa gacaaggaaa accctgccta cgtggtagtt ggtctggatt ggcaagtcga
     9601 ctatgaaaaa tttgccacag caactcttgc tattcaaaag ggtgcccatt ttatcggaac
     9661 caatcctgac ctcaacatcc cgacggaacg tggtcttttg ccaggtgctg gctccctgat
     9721 tacgctgctt gaagtagcga cacgagtgaa gccggtttat atcggaaaac ccaatgccat
     9781 cattatggac aaggcggttg agcacttagg tttggaacgt gaagagttaa ttatggttgg
     9841 ggacaattat ttgactgata tccgggctgg gattgacaat ggcattccaa cgctcttggt
     9901 gacgacaggt tttactaagg cagaagaagt agctggcctg ccaatcgcac cgacacatgt
     9961 ggtttctagc cttgcggagt gggattttga tgaaaactaa attgatcttt tggggctcta
    10021 tgctctttct cctctccctc tcaatccttc tgaccattta tctggcttgg attttctatc
    10081 ctatggagat tcagtggcta aacttaacga atcgagtcta tctaaaacca gaaaccattc
    10141 aatacaattt tcatatcttg atgaattatc tgaccaatcc ttttagtcag gtcttacaga
    10201 tgcctgattt tcgttcgtca gcagctggtc tgcaccattt cgcagtggtc aagaatctct
    10261 ttcatttggt tcagctagta gctctagtga cactgccaag tttctatgtc tttgtcaata
    10321 gaattgtgaa aaaggacttt ttgtctcttt atcgaaaaag tctcctgact ctagtagtct
    10381 tacctgtgat gattggactt gggggagttt tgattggttt tgaccaattc tttactcttt
    10441 tccatcaaat tctctttgtg ggagatgata cctggctttt tgatccagcc aaggatcctg
    10501 ttattatgat tttgccagag accttctttc ttcatgcctt cctcctcttt tttgccctct
    10561 atgaaagctt ctttggctat ctgtgtctga aaagtcgtag gaaatgaaat gaaagatgtt
    10621 tttaatttgc atatataaaa atatatatcg tttaaaacca tcgttaagag tgaattaacc
    10681 aaatccaatt gtgtcaatcg ttcgcaaaaa gcctattttt cttgaaaaat aggctttttt
    10741 ttctaaaaat ccctagtcaa gcgctttatt tttttgtata atagaagtag caaagtatag
    10801 ataagaagag aaaagcatga ttacactatt tctatcaccg agctgtacat catgtcgtaa
    10861 ggctaaggcc tggttagaaa aacataaggt tccctttgtg gaacacaata ttatgaccag
    10921 tcctttaaca agaaaagaat tgcaacacat cctttccttg accgaaaatg gtactgatga
    10981 catcatttca actcgttcaa aaatttttca aaaattgaat attgatgtag agagtatttc
    11041 ggtatcggaa ttgcttcatt tgattgagca gtatcctagt cttttgcgtc gtccaattat
    11101 tattgatgcc aagcgtatgc aaatcggttt taatgaagat gagattcgtg cttttctccc
    11161 tcgtagttat cgtaagcaag aactaaaaga agcaagaatg agagctggta ttagttaatg
    11221 aacaaacagt atagttaccc actagatttg tcgtggagca ctgaagaact tgcttcagtg
    11281 ctttcttttt ttaatgatgt tgaagctgcc tatgaaggca aagtagaggc taaaaagtta
    11341 ctggactcct ataagggatt caaagcggtc gtgccaagca agagcgaaga gaaacgtttg
    11401 ggacgagaat ttgaaacggt tagtggctat tcactttacc gagcagttca ggctgccaaa
    11461 gaaaaagggg aaggaaagat ttctcttgga aagtaaattt gaatttgcta aagagcttgt
    11521 taagaaagca ggtcagtaca ttcttgacca tatgcaggaa gacttgcgtg ttgaaaccaa
    11581 gtcctctcca acagatttgg tgaccagact ggacaaagaa gttcaggaac tcttggttgg
    11641 tgagattttg tcccgttatc ctgaggataa gatttgtgct gaagaaggtt gtctgcgagc
    11701 ctcggttcaa gagggcaagg tttgggtcat tgatcccatt gatggtacca ataattttgt
    11761 agcccagcag gaagattttg ctgttatgat ggcttatttt gaaaatggtc agggacagtt
    11821 tggtctgatt tatgatgtgg tcaaagggga ttgttaccac ggtggtgggg aatttccagt
    11881 ttgtctaaat gataggcccc tagcttcttt taaaactaaa ccacttggag attttctcat
    11941 tgcagggaat agtggtatgc tggaaaccaa tgaatggggg ctggctgatt tgagccgagc
    12001 ggttcttggt gttcgtgtct atggaagtgc ggccattagt tttgccaaga ttttgtcagg
    12061 gcgtttgttg acctatctca cctatctgca accatgggat tatgccgcag ctagtatttt
    12121 aggggaaagt ctgggctatc gggttgtgac cctttttggt gaagctcctg attttcaaac
    12181 cagacagccg gtcatgatgg tgcctcttga gatgcaggag gaaattcagt cctatattta
    12241 cgaaaggaaa agaacttaaa tgaaatttcc agaaggattt gttgaaaaat ataaagaaat
    12301 actaggagat gaggcaagag attttcttgc ctcttttgag gaggaagcgg tttcggcctt
    12361 tcgggtcaat cccttaaaag aagagcaact ttccttttct gatgccatta ctcaaacccc
    12421 ttggggccac tatgggaagg tttcagggaa atcgcctgag catgctacag gtttagttta
    12481 ttcgcaagaa cctgctgctc aaatggtggc tcaagtagcc caacccagtc ctggtatgaa
    12541 ggtcttggac ttggctgctg caccgggggg caaatcaact caactggcag cctatctagc
    12601 aggggagggg ctacttgttt ccaatgaaat ttcaagcaaa cgggctaaga ttttggttga
    12661 aaacatggag cgttttggag cgacaaatgt cgtggtgacc aatgaatctg ccgaccgctt
    12721 ggtcaaggtc tttaaaggct attttgactt aatcgttctt gatgcccctt gctctggtga
    12781 aggaatgttt cgtaagcaac ctgatgctat ggactattgg agcttagatt atccgagtca
    12841 atgtgctagc ttgcaaagag aaattctgga ggatgcagtg accatgttag ctgaaggtgg
    12901 tcgtctggtt tattcgacct gtacctgggc acccgaggaa aacgaagaga ttgtcaattg
    12961 gttgctggaa gagtatgatt ttgacttgtt gccagtagag catatcaatg gaatggtagc
    13021 tggtattgac ctgccagaaa cggctcggat gtaccctcat cagtttaagg gagagggtca
    13081 gtttgttgcc catctacagt ttaagggtaa caatccagct cctaaattta aggcaagtaa
    13141 gagtaacctc agccgtgaac aagttgcctt gtggcaggaa tttgcccaaa accatttgaa
    13201 ggtcaatcta ccgggtatct tgcagatttt tggagaccag ctttatctct tgccagaact
    13261 tttgccagat ttagggaagc tcaagattgc tcgcaatgga ctgcatctgg gtacttttaa
    13321 gaagaaacgc tttgagccca gtttcgctct tggtttagcc ttgaaaccga gtcaggtcga
    13381 acagtcggtt gaaattggcc aagaagagtt tgtcaagtat gcggctggag aaaccgttca
    13441 gctggcagaa agtctgccaa atggttggta ccaagttttg gttaaaggca atggcttggg
    13501 ctttgcaaag gttactggaa atgttttgaa aaattatttt ccaaaaggcc tcagattcaa
    13561 atgaaaatgc tagtcaaata tcttattcta tgttatagtg aaagtatgga tttttttagt
    13621 aggatgattc ttgatatctt gattgccatc acatgtagtt ttactactcg atgaaaatca
    13681 aagagcaaac taggaagcta gccatagata gctcaaaaca ctgctttgaa gagattttca
    13741 aagagtacaa gaagtttagt tattagcgtt cttaccgctt gtaaactaga tttctcataa
    13801 aatagaatct tttcctttta gttgtaaact agtctgggag agtagagaga tttgagatac
    13861 ctttctagct tttagattat catctaagga gagtaatttc ccttgcatta aaaaggggaa
    13921 aaagagacac gaaatgacta taatgggtga caatggggga agggatagac aagagatttt
    13981 atccacatat gaaaaaagga ggttaggaaa gagtaatata gcctatatta tataaataat
    14041 caattgcgca gaaatttggt aagaattcat gcgtcaactc ataaagaact acttaaaaaa
    14101 ttcacagtat tcataattat tttcgaggag aaaaacagtg aaaaaaagaa aaaagcttgc
    14161 tctgtctctt atcgcttttt ggctgacggc ttgtttagta ggctgtgcta gctggattga
    14221 tcgtggagaa tccataacgg ctgttggctc aactgccttg caacccttgg ttgaagtagc
    14281 ggcagatgaa tttggcacga tccatgttgg aaaaacggtc aatgtccaag ggggaggttc
    14341 tggtacaggc ttgtcccagg ttcagtctgg ggcagttgat ataggaaact cagatgtatt
    14401 tgctgaggaa aaagacggaa ttgatgcttc tgctcttgtt gaccacaagg tcgcggtagc
    14461 tggcttggct ctgattgtca ataaggaggt tgatgttgat aacctaacga cagagcaact
    14521 tcgtcaaatc ttcataggtg aggtaaccaa ttggaaagag gttggtggta aggacttacc
    14581 catctctgtt atcaatcggg cagccggctc tggctctcgt gctacctttg atactgtcat
    14641 tatggaaggt cagtctgcca tgcaaagtca ggagcaggat tcaaatggag cggtaaaatc
    14701 aatcgtatca aaaagtccag gagctatctc ttatttatct cttacctata tagatgattc
    14761 ggtcaaaagc atgaagttga atggctatga cttaagtcca gaaaatataa gtagcaataa
    14821 ttggcccttg tggtcttatg agcatatgta tacattgggg cagcccaatg agttagctgc
    14881 agaatttctc aattttgttc tctcggatga gacccaagaa gggattgtca aaggattgaa
    14941 gtatattccg attaaggaaa tgaaggttga aaaagatgct gccggaactg tgacagtgtt
    15001 ggaagggaga caataatgaa tcaagaagaa ttagctaaga aaatgttgct tccatcaaag
    15061 aattctcgtc tggagaaatt aggaaaaggt ttgacctttg cctgtctttc tttgatagtc
    15121 atccttgtgg ccatgatttt ggttttcgta gcgcaaaaag gcttgtcgac cttctttgtc
    15181 aatggtgtga atatctttga ctttcttttg ggaggaactt ggaatccttc tagtaaagaa
    15241 tttggtgccc ttcctatgat tttgggttcc tttatcgtta ccattctctc agcccttatc
    15301 gcaacaccct ttgctattgg tgcagcagtt tttatgaccg aagtatcacc aaaaggggcg
    15361 aagattttac aaccagctat tgaactcctg gttgggattc cttcagtagt gtacggattt
    15421 attggcttgc aagtcgtcgt tccctttgtt cgcagtgtct ttggtgggac tggttttggg
    15481 attttgtcag ggatttccgt cctctttgtc atgattttgc cgaccgtaac ctttatgaca
    15541 acggatagct tgcgtgcggt tcctcgttat tatcgtgagg ccagtttcgc tatgggagcc
    15601 actcgctggc agactatctg gcgtgtaatc ttgaaggcgg cccgttctgg tattttcact
    15661 gcagtggtct ttgggatggc gcgtgccttt ggtgaggctt tagctatcca gatggttgtc
    15721 ggaaactcag ctgttatccc aacttccttg accacaccag ctgcaacttt aacttctata
    15781 ttaactatgg gaattgggaa cactgtcatg ggaactgtaa ataataatgt tctctggtca
    15841 ctggccttgg tactgctctt gatgagttta gtttttaaca gtgtgattaa attgattacg
    15901 aaagaaagag ggaagaaaaa ttatgcgcgc taaaaaatta gataaacttg caacagctgt
    15961 cctctatacg attgctagca tcattgtgac aatcttggct tccttgattc tctatatctt
    16021 ggttcggggc ttgccccata tctcttggtc tttcttgact ggaaggtctt ctgcttttca
    16081 agcaggtggt gggattggca ttcagcttta caattccttt ttcctattgg tcattacctt
    16141 gattatttct gtacctcttt ctatgggagc tgggatttac ttggctgaat atgctaaaaa
    16201 aggtcctgtt accaactttg tgcggacttg tattgaaatc ttgtcctctt taccatcagt
    16261 ggtggtgggt ctctttggtt acttgatctt tgtagtccag tttgagtatg gattttcaat
    16321 catttcaggt gccttggcct tgacagtctt taacttgcct cagatgacgc gtaatgtaga
    16381 agatagtttg aaacacgttc accataccca acgtgaggct ggtctggctc ttgggatttc
    16441 tcgctgggag acagtggttc atgttgttat tccagaagcg cttccaggta ttgtaacggg
    16501 tgtcgtcttg gcatctggtc gtatctttgg cgaagctgca gctctgatct atacagcagg
    16561 gcaatcggcg ccagctcttg actggtctaa ctggaatatc ctcagtgtga ctagccccat
    16621 ctctatcttc cgtcaagcag aaaccttggc tgtccatatc tggaaagtca atagtgaagg
    16681 cactattcca gatggaacaa ttgtatcagc aggttctgcc gctgtgctcc tgatctttat
    16741 cctgattttt aactttggag ctcgtaagtt cggaagctat ctacacaaga aattaaccgc
    16801 tgcctaaagg agaagccatg tcaacatata actgggatga gaagcatatc cttacctttc
    16861 ctgaagaaaa agtagccctt tctactaagg atgtccatgt ttactatggt aaaaatgaat
    16921 ccattaaggg gattgatatg caatttgaaa gaaataaaat tacagctttg attggcccgt
    16981 cgggatcggg gaaatctacc tacttacgca gtctcaatcg catgaatgat actattgata
    17041 ttgctaaagt aactgggcag attctctatc gtggaattga tgtcaaccgt ccagaaatca
    17101 acgtttatga aatgcgtaaa cacattggaa tggtttttca acgccccaat ccatttgcta
    17161 agtcaattta ccgtaatatt acctttgcgc atgaacgtgc tggagttaag gataagcaag
    17221 tcctagatga aatcgtagaa acctcccttc gtcaggctgc cctctgggat caggttaaag
    17281 acgatctcca caagtcagcc ttgaccttat caggtggtca gcaacaacgt ctctgtatcg
    17341 ctcgtgccat ctctgttaag ccagatatcc tcttaatgga tgagccagcc tcagccttgg
    17401 atccgattgc gaccatgcaa ctagaagaga ccatgtttga gctcaagaaa aactttacca
    17461 tcatcattgt aacgcataat atgcagcagg ctgctcgtgc aagtgactat acaggcttct
    17521 tttacttggg tgatttgatt gagtatgaca agactgcaac tattttccaa aatgccaagc
    17581 tacagtccac caatgactat gtatctggtc actttggtta gaaaggaaac cgtatgacag
    17641 atgcgatttt acaggtatca gacctgtccg tttattataa taaaaagaag gctttgaata
    17701 gtgtttccct atctttccaa cctaaggaaa ttacagcctt gattggtcca tctggatcag
    17761 ggaagtcaac cctcctcaag tctctcaacc gcatgggaga tctcaatcca gaggtgacca
    17821 caactggatc cgtggtgtac aatggccaca acatctacag tccgcgtaca gatacggttg
    17881 aattacgtaa ggaaatcgga atggttttcc aacaacctaa tcctttccct atgactatct
    17941 atgagaatgt tgtctacggg cttcgtatca atggaattaa ggataagcag gttctggatg
    18001 aagccgtaga aaaagccttg caaggtgcct ctatctggga tgaggtcaag gatcgtctat
    18061 atgattcagc tattggattg tcaggtggtc aacagcagcg tgtctgcgtg gcccgtgtct
    18121 tggcaactag tcctaaaatc atcctcttgg atgagccaac ttcggctttg gatccgattt
    18181 cagctggtaa aattgaggaa accttgtatg gtctaaaaga caagtacacc atgcttctgg
    18241 taacccgttc catgcagcaa gcttcacgta tctctgataa gacaggattt ttcctagatg
    18301 gagatttgat tgaatttaat gataccaagc agatgttcct tgatccccaa cacaaggaaa
    18361 cggaagacta tattacagga aaatttggat aaggagatga aagatgttac gatctcaatt
    18421 tgaagaagat ttagagaaat tacataacca gttctacgct atgggacaag aagtgctctc
    18481 acaaatcaat cgtacggtac gtgcttttgt cacgcatgac cgtgacctgg caaaagaggt
    18541 catcgaagat gatgcagaag taaatgaata cgaagtgaaa ctggaaaaga aatcatttga
    18601 aatgatcgca ctccaacaac cagtctctca agatttgcgt acagtcttga ctgtccttaa
    18661 ggctgtatca gatgtggagc gtatggggga tcacgctgta gccattgccc aggcaaccat
    18721 ccgtatgaag ggggaagagc gcattccagc tgtagaggaa gaaattaaaa aaatgggacg
    18781 tgaagttaaa agcgttgttg aagcagcact tgatctttat cttaatggtt ctgttgacga
    18841 cgcataccgg gtggcctcca tggatgagca aattaaccac tattttgaaa ctatccgtga
    18901 ccttgcgact gaagagatta agaaaaatcc agaagccatt gttacgggtc gtgattattt
    18961 ccaagttatt tcctacttgg agcgtatcgg tgactatgct aaaaatatct gtgaatgggt
    19021 tgtctacttt gaaacaggta agattgtcga actataaaat agtttaattc tgtataaaaa
    19081 ggagcttgaa atcaactgat ggctcctttt tttgaacgaa aaaatatttt tgagataaaa
    19141 atttaaaaaa cacttgacaa gtaacttgga tatcgttata attatacaaa attaacagag
    19201 aggttgttta tttatgaaat caaaaaaatg gatatttgtt ttatgtaatt ttcttgcaag
    19261 tttcttctta gtagcttgcc agtcaggttc taatggttct cagtctgctg tggatgctat
    19321 caaacaaaaa gggaaattag ttgtggcaac cagtcctgac tatgcaccct ttgaatttca
    19381 atcattggtt gatggaaaga accaggtagt cggtgcagac atcgacatgg ctcaggctat
    19441 cgctgatgaa cttggggtta agttggaaat cttaagcatg agttttgaca atgttttgac
    19501 cagtcttcaa actggtaagg ctgacctagc agttgcagga attagtgcta ctgacgagag
    19561 aaaagaagtc tttgattttt caatcccata ctatgaaaac aagattagtt tcttggttcg
    19621 taaggctgat gtggaaaaat acaaggattt aactagccta gaaagtgcta atattgcagc
    19681 ccaaaaaggg actgttccag aatcaatggt caaggaacaa ttgccaaaag ctcaattaac
    19741 ttccctaact aatatgggtg aagcagtcaa tgaattgcag gctggaaaaa tagatgctgt
    19801 tcatatggat gagcctgttg cacttagtta tgctgctaaa aacgctggct tagctgtcgc
    19861 aactgtcagc ttgaagatga aggacggcga cgccaatgcc gttgctctta gaaaaaatag
    19921 tgatgatttg aaagaagtgg tagacaaggt catccaaaaa cttaaggatg aaggaactta
    19981 ccaaagctat cttgaaaaag cagcaagttt aactgaagtt gaagaataag aaaaagcaga
    20041 gttggatgtc attccaattc tgcttttaaa tagtttaaaa cattttccag attgtctagg
    20101 gacaccttgg caaattgata aggacaggtg cttaaatagg cctcttcaaa gaagtccaag
    20161 cccagttgac tgtgtttgag actggcgatt ttaggatact gtctcaggtc tagcaataaa
    20221 ccatcgtgta ggggataatg aggaagggga caaggtgttt tagttatcct ttcctcgatt
    20281 tgtttttggt aagattcatg attggtcaag cgagccaaac gatttttttc aaacaattga
    20341 ctgtcaatat agagtgacag ggccttttgc atgatgaggt tgctgtcgta gtctaggata
    20401 tttttgtagg ccacgaaggc ttctttgata gtatttggaa gaatgagtgc tgttatccgc
    20461 aaggctggaa agaggctggt tgagaaggat ttgatataaa tgacgcgttc ctctgtatct
    20521 agatagtgga aggtctggcc ctttttagag tccaaatccc ccagatagtc gtcttcgacg
    20581 atataaacac tatatttggc tgctaagtca agaatagctc gtttgtcctg ctcagaatag
    20641 gaatggccta ggggatagtg aaatcgagga atggtgtaga aaaacttaat ttttcctgtt
    20701 ttgaagtggc cttccagctc ctccaagtca atcccatcaa tgcctcgttc aatcgtttga
    20761 tagtccagcc cctgtgcaat caagaggcga ttcatccgat ggtaggttgg ctgttccacc
    20821 aagatttcct tggcttggct aggaaaggat atttgagaga ggataaacaa ggcttgttgg
    20881 gttccagaag tcagtactag ttggttagcc ttgcagtaga gagcttggtc aaagaggagt
    20941 ttgtgaatgg attgtcttag gtcttctaat ccttcttgat tgtcatagta gttgaagagg
    21001 tagttttctc ggccaatcaa ggtttcattg acacagagtc ggaaatcgtc ataggcactg
    21061 gcatgttcgt cggtaacctc gatttctagg tcttgatgtt gcccttgttc taatacatag
    21121 tagccactct gaggcttggc atagaggtat tgttcgtgcc gtaattccag cagggctcgt
    21181 tgaatggtgt ccttgctgca gtgaaagtca aggctcagtt gacggataga aggcaggcga
    21241 ctacccgtcg gaaatcgtcc agactcgata ccatttttca gataggaaac gacctctttg
    21301 tacttgcttt gtttcttcat tccagacctc ccttgatttt gttacattgt accctttttt
    21361 ttccttcttg gcaatgtcta gagcgtttct ctcgttcaca tctaggggca aatgtggtat
    21421 acttaggaat aagatttata gaataacaga caagaaagtg aatggataag aacgtgcagg
    21481 aaccagtgaa attatttcaa tacaatactc ttggagcctt gatggctggc ctttatggtg
    21541 gtactatgac agtaggagaa ttgctagaac atggtgacct tggtttggga accttggatt
    21601 ccattgatgg ggaattgatt gtcttggatg gcaaggctta tcaggccaaa gagtcaggag
    21661 accagccaga aattgtggaa gtgtcaccag atgcccttat tccttacgct gcagtggtac
    21721 cgcatcaggc agaggtcatt ttccgccagc gctttgagat gacagacaag gaattgaaag
    21781 aacgaattga gtcttattat gatggggaaa atcttttccg ctctatcaag attcgggggg
    21841 aattttcaca tatgcatgtg cgtatgattc ctaagtcaac acccgatacc aagtttgctg
    21901 atatcgcaac ccatcaaccg gaatatagtc gtgacaatgt tgcggggact attgttggtt
    21961 tctggacgcc tgagattttc catggggtca gtgtggcagg ctaccatctg cacttcatat
    22021 cagatgattt gaccttcggt ggacatgtca tggattttgt catcaaggaa ggcattatcg
    22081 aggtgggagc agttgaccag ttggaccaac gtttcccagt ccaagaccgt caatacttgt
    22141 ttgctaagtt caatgttgac gagatgaaaa aagatattga aaaggcagaa taggagaaga
    22201 aaatgaccat tcatatcatt attaccatgt tgctgttgct agcttttctg ataggaagca
    22261 tttggtttgc caaaaagaaa taccagatta atctagctgt cttgggcttg ggtgcagtcg
    22321 ctttctttgt ctcttcgcag attttggaaa aactggtgca tatcttgatt ttgcatcctc
    22381 aaaaagacgg tagtattgcc ctcttgcaag atcatccgct tatctatatt atctatggcc
    22441 tagccatggc agcatttttt gaggaaacgg ctcgtcttgt tttcttcaaa tggttggaga
    22501 aaaagagaag tttggaaaag gcagatgcct tggcctatgg tctagggcat ggtggcttgg
    22561 agttgatttt tctaggcctt actagtttac tcaatcttta catcgttctt tcggcagttc
    22621 aaactcagaa tccacaggtt atgcaattac tgtctgaaaa tatgttaaaa accattcagt
    22681 cactttcggt ctggcagatt tatttgcttg gttttgagcg aatcttggcg cttggtttcc
    22741 aattactttt gacagtttgg gtttaccaag ctgttcgcca gaagaaatgg atttatctcc
    22801 tagcagccta tggcttgcat gccttctttg atctggcacc atctcttttc caagtaggct
    22861 ggttgacaaa tccagtcttg gttgaagtga ttctagcact ggagctcgtt ctggttgcct
    22921 atggaaccaa ggaaatcttt tgtaaaaaat cataaaaagg ggggaacctc tttttcttat
    22981 gcaaaatcca aacaaggtat ttttatggtc gtcaaatgtc tctaaaaatg gtataatgga
    23041 atgaattttg taaaaggaag agtgacatgt ctgtaagaga aaaaatgctt gaaatcttag
    23101 aaggaattga tatccgtttt aaggaaccct tgcatagcta tagttataca aaagtaggtg
    23161 gagaggctga ttatttggtc tttccacgaa atcgttttga gttggctcgc gttgtgaaat
    23221 ttgccaacca agaaaatatc ccttggatgg ttcttggcaa tgcaagcaat atcatcgttc
    23281 gtgatggtgg gattcgtgga tttgtcatct tgtgtgacaa gctcaataac gtttctgttg
    23341 atggctatac cattgaagca gaagctgggg ctaacttgat tgaaacaact cgcattgccc
    23401 tccgtcatag tttgactggc tttgagtttg cttgtggtat tccaggaagc gttggcggtg
    23461 ctgtctttat gaatgcgggt gcctatggtg gcgagattgc tcacatattg cagtcttgta
    23521 aggtcttgac caaggatgga gaaatcgaaa ccctgtctgc taaagacttg gcttttggtt
    23581 accgccattc agctattcag gagtctggtg cagttgtctt gtcagttaaa tttgccctag
    23641 ccccaggaac ccatcaggtt atcaagcagg aaatggaccg cttgacgcac ctacgtgaac
    23701 tcaagcaacc tttggaatac ccatcttgtg gctcggtctt taagcgtcca gtcgggcatt
    23761 ttgcaggtca gttaatttca gaagctggct tgaaaggcta tcgtatcggt ggcgtagaag
    23821 tgtcagaaaa gcatgcagga tttatgatca atgtcgcaga tggaacggcc aaagactacg
    23881 aggacttgat ccaatcggtt atcgaaaaag tcaaggaaca ctcaggtatt acgcttgaaa
    23941 gagaagtccg gatcttgggt gaaagcaagt aggaagctgt atttgaaaag ctgctgctat
    24001 gtcatggaaa gggggtgaaa gcctatcggt agcgaagatg tatgcaggtg gttttactcc
    24061 ctgcaagagg tagtggggac ctgacagagc cccgatcggt taatctatga aaaagaagga
    24121 atttatgcca attgaaaaaa ccaattatcg aattcaaaaa cgtctctaaa gtttttgaag
    24181 acagcaacac caaggttctc aaagacatca actttgagtt ggaagaaggg aaattctaca
    24241 cccttctagg tgcatctggt tcggggaaat caactatcct aaacattatt gcaggtttac
    24301 tggatgcgac gacaggagat atcatgctag acggtgttcg tatcaatgat attccaacca
    24361 acaagcgcga cgtacatacc gtcttccaat cctatgcctt gttcccacat atgaatgtgt
    24421 ttgaaaatgt tgcctttcca cttcgcttgc gtaaaattga taagaaagaa atcgagcagc
    24481 gtgtagcgga agttctcaag atggttcagt tggaaggtta tgaaaaacgt tccatccgca
    24541 aactttctgg aggacaacgt cagcgtgtgg ccatcgcccg tgctatcatc aaccaacccc
    24601 gtgtggtctt gttggacgag cctttatcag cgctggactt gaaattgaga acagacatgc
    24661 agtacgaatt gcgtgaatta caacaacgat tgggcattac ctttgtcttt gtcactcacg
    24721 atcaggaaga agctcttgcc atgagtgact ggattttcgt tatgaatgat ggcgagattg
    24781 tccagtctgg aacccctgtg gacatctacg atgagccaat caaccacttt gttgccacct
    24841 ttatcgggga gtcaaacatc ttgccaggta ccatgattga ggactacttg gtcgaattta
    24901 acggcaaacg ctttgaagcg gttgatggtg ggatgaagcc aaatgaacct gttgaggtcg
    24961 ttattcgtcc agaggacttg cgcattaccc ttcctgaaga aggcaaactc caagttaagg
    25021 tcgacaccca gctcttccgt ggagttcatt atgaaattat cgcctatgac gaacttggaa
    25081 atgaatggat gatccactca acccgtaagg ccatcgtggg tgaggaaatc ggtctggact
    25141 ttgaatcaga agacatccac atcatgcgtc tcaatgaaac cgaagaagag ttcgatgctc
    25201 gtatcgaaga atacgtggaa atcgaagagc aagaagcagg tctgatcaat gcgatcgagg
    25261 aggaaagaga tgaagaaaac aagctctaaa ctctttgtag tgccctacat gctttggatt
    25321 gccctctttg tattggcacc cttggtcttg attttcggac aatccttttt caacattgaa
    25381 ggccagttta gtttagaaaa ttataaatct tactttgcgt cacaaaactt gacctacctc
    25441 aaaatgagtt tcaactcagt gctttatgca gggattgtga cctttgtgac cttgcttatc
    25501 agctatccaa cagccctctt tttgacccgt ctcaagcacc gtcaactctg gctcatgctg
    25561 attatccttc ctacctggct caatttgctc cttaaagcct atgcttttat cgggattttt
    25621 ggtcaaaatg gctctattaa ccaattcttg gaatttatcg gaattggttc acaacagttg
    25681 ctttttaccg atttttcctt tatctttgtc gcaagctaca tcgagctccc ctttatgatt
    25741 ttgccgattt tcaatgtctt agacgatatg gataataatc tcatcaatgc tagttatgac
    25801 cttggtgcca ctaagtggga gaccttccgt catgtcatct tccctctatc tatgaacggt
    25861 gtgcgaagtg gggttcagtc ggtctttatc ccaagtttga gtctcttcat gctgacccgt
    25921 ttgattggtg ggaaccgcgt tatcaccttg gggacggcta ttgagcagaa ttttctaacc
    25981 aatgacaact atggtatggg ttcaaccatc ggtgtgattc tcatcctgac catgttcatc
    26041 accatgtggg tgactaagga aaggagagaa cgatgaaaaa atttgccaac ctttatctgg
    26101 gactggtctt tctggtcctc tacctgccta tcttttactt gattggctat gcctttaatg
    26161 ctggtgatga tatgaatagc tttacaggtt ttagctggac tcactttgaa accatgtttg
    26221 gagatgggag actcatgctg attttggctc agacattttt cttggccttc ctatcagcct
    26281 tgatagcgac cattatcggg acttttggtg ccatttacat ctaccagtct cgtaagaaat
    26341 accaagaagc ctttctatca ctcaataata tcctcatggt tgcgcctgac gttatgattg
    26401 gtgctagctt cttgattctc tttacccaac tcaagttttc acttggcttt ttgaccgttc
    26461 tatctagtca cgtggccttc tccattccta tcgtggtctt gatggtcttg cctcgactca
    26521 aggaaatgaa tggcgacatg attcatgcgg cctatgactt gggagctagt caatttcaga
    26581 tgttcaagga aatcatgctt ccttacctga ctccgtctat cattactggt tatttcatgg
    26641 ccttcaccta ttcgttagat gactttgccg tgaccttctt tgtaacagga aatggctttt
    26701 caaccctatc agtcgagatt tactctcgtg ctcgcaaggg gatttcctta gaaatcaatg
    26761 ccctgtctgc tctagtcttt ctctttagta ttatcctagt tgtaggttat tactttatct
    26821 ctcgtgagaa ggaggagcaa gcatgaaaaa aatctattca tttttagcag gaattgcagc
    26881 gattatcctt gtcttgtggg gaattgcgac tcatttagat agtaaaatca atagtcgaga
    26941 tagtcaaaaa ttggttatct ataactgggg agactatatc gatcctgaac tcttgactca
    27001 gtttacagaa gaaacaggaa ttcaagttca gtacgagact tttgactcca acgaagccat
    27061 gtacactaag ataaagcagg gtggaacgac ctacgatatt gccattccaa gtgaatacat
    27121 gattaacaag atgaaggacg aagacctctt ggttccgctt gattattcaa aaattgaagg
    27181 aatcgaaaat atcggaccag agtttctcaa ccagtccttt gacccaggta ataaattctc
    27241 catcccttac ttctggggaa ccttaggaat tgtctacaac gaaaccatgg tagatgaagc
    27301 gcctgagcat tgggatgacc tttggaagct ggagtataag aattctatca tgctttttga
    27361 tggggcgcgt gaggtgctgg gactaggact caattccctc ggctacagcc tcaactccaa
    27421 ggatctgcag cagttggaag agacagtgga taagctctac aaactgactc caaatatcaa
    27481 ggctatcgtt gcggacgaga tgaagggcta tatgattcag aataatgttg caatcggcgt
    27541 gaccttctct ggtgaagcca gccaaatgtt agaaaaaaat gaaaatctac gttatgtggt
    27601 accgacagag gccagcaatc tttggtttga caatatggtc attcccaaaa cagttaaaaa
    27661 ccaaaactca gcctatgcct ttatcaactt tatgttgaaa cctgaaaatg ctctccaaaa
    27721 tgcggagtat gtcggctatt caacaccaaa cctaccagcg aaggaattgc tcccagagga
    27781 aacaaaggaa gataaggcct tctatcccga tgttgaaacc atgaaacacc tagaagttta
    27841 tgagaaattt gaccataaat ggacagggaa atatagcgac ctcttcctac agtttaaaat
    27901 gtatcggaag taggagataa acgtaagaag acgaatcagt caagctggtt cgtttttaag
    27961 gaatagaagt cagtttttag agccctatct ttttctattc tagttgttta tttgtttatt
    28021 tgcttatttg cttgcctagt agtatactaa gcctatactc ttcaaaccac gtcagcgttg
    28081 ccttgccgta ctcaagtaca gcttgaggct agcttcctag tttgcttttt gatttttatt
    28141 gagtattaac cttacagaaa gcggcaacaa ataatgaaaa tgtctacttt tttcaaaaaa
    28201 agtttctggc caacctttat gattgttaac caaactgtta tactattcca tttaaaggat
    28261 ggcttggatc gacaatacct aacaactgag tccatatatt gggtgatagg cacctttata
    28321 tttggaaata ttctagtagc tgtgtttagt aatatgaaaa tttgggataa aaagaaaaac
    28381 ggcagtaaga aaaaatatat cctgaaaaag taatcagaga tgtagggaat tggtctaatt
    28441 caacggagat catttccagt tgacgcaagc ttaaaaaaac gttataataa gaaagttgag
    28501 aattgatttt cacttgtctt agtccagaga aatggcggtg ctgcgagcca tctaagctag
    28561 gaagtcatgc tactcaatca tacaattgca taagaacaag agaatgacaa gttcattgaa
    28621 tgaaggtggt accgcggttt ttcgcccttc gtgatatgag cttgtctttt gatttttgga
    28681 ggtgttgatg aaaacatttc ttgtcaaaca aaagtttcgt cttggaggcg aacgcttcgc
    28741 tatcaaggat gacaggggag aaattgccta tcaggtggag ggatcatttt ttaagattcc
    28801 taaaaccttt accatctatg atgcggctgg tgaacaagtc agtgagatta gtaaagaaat
    28861 cttgaccttg cttcctcgtt ttgagattca gcttcgggat ggctcgagtt ttgtcattcg
    28921 taagaagttg accttctggc gagataagta tgagtttgat aatctaggtc ttcgtatcga
    28981 gggcaatatc tgggatttga atttcaaatt gctggatgat cgcgatcagc tgattgcgga
    29041 aattaagaag gaactcttcc atctgacctc tacctatacc gtaacggttc ttgaggacgc
    29101 ttatgcagac ctagtcattt ccctctgtgt cgcgattgac tatgtggaaa tgctggaaag
    29161 ccaatcacat taaacaagta aataaggaga caatatgaaa caactatcta gtgcacaagt
    29221 acgccaaatg tggcttgatt tctgggcgac caaagctcac tcagtagaac catcagtaag
    29281 tttggttcct gtaaatgacc caactctttt gtggatcaac tctggggtag caacccttaa
    29341 gaaatacttt gacgggacca ttatccctga aaatccacgt attaccaatg cccaaaaggc
    29401 tatccgtacc aacgacatcg aaaacgtagg gaagactgcg cgtcaccata ccatgtttga
    29461 aatgctgggg aacttctcta tcggggatta cttccgtgac gaagctatca cttgggctta
    29521 tgagcttttg acaagccctg aatggtttga tttccctgct gaaaaacttt acatgaccta
    29581 ctatccagac gataaagatt cttacaaccg ctggattgaa gtgggagtgg attcaagtca
    29641 cttgattcca attgaggaca acttctggga aatcggtgcg ggaccttctg gaccagatac
    29701 agaaatcttc tttgaccgtg gggaagcctt tgacccagaa aatatcggtc ttcgcctgct
    29761 tgcagaagat attgaaaacg accgttatat tgaaatctgg aacatcgttt tgtcacaatt
    29821 taacgcagac cctgctgttc ctcgtagcga atacaaggaa ttgccacata agaacattga
    29881 tacgggcgct ggtttggagc gtttggtggc tgttatccaa ggggctaaga ccaactttga
    29941 aacggacctc ttcatgccga ttatccgtga agtcgagaaa ttgtctggta aggtttatga
    30001 ccaagatggc gacaacatga gcttcaaggt tatcgcagac cacattcgtt ctctttcatt
    30061 tgctatcggt gatggtgccc ttccaggaaa tgaaggtcgt ggttatgttc ttcgtcgtct
    30121 tctccgtcgt gcttctatgc atggtcaaaa attgggtatc aacgagcctt tcctttacaa
    30181 acttgttcca actgttggaa aaatcatgga aagctactac ccagaagtgc ttgagaaacg
    30241 tgactttatt gagaaaatcg ttaagagcga agaagaatca tttgcccgta cccttcactc
    30301 aggtcaacac tttgcccaag gcattgtggc agacttgaaa gaaaaaggtc aatctgtcat
    30361 tgctggtcaa gatgtcttta aactctatga tacatacgga ttcccagttg aattgactga
    30421 agaaattgct gaagaagctg gtatgactgt agaccgtgaa ggttttgaag cagccatgaa
    30481 agaacagcaa gaacgcgcgc gtgcgtcagc tgtcaagggt ggctcaatgg gtatgcaaaa
    30541 tgaaactctt caaaacatca ctgtagaaag tgtcttcaac tacaatacta gccaattgtc
    30601 ttctaaattg gtagctatcg ttgctgacaa tgcagaagta ggagctgttt cagaaggaac
    30661 tgcctctctt atctttgcgg aaacgtcatt ttatgctgaa atgggtggac aggtagctga
    30721 ctacggacaa atcttggatg agtcaggtaa gattgtggct actgtgacca atgttcagaa
    30781 agccccaaat ggtcaagccc ttcatacagt tgaagtcctt gcaccgcttg ccttgaacca
    30841 agaatatacc ttggcaattg atagcaatcg ccgtcaccgt gtcatgaaaa accacactgc
    30901 gactcatttg cttcacgctg cccttcacaa tatccttgga aaccacgcaa cacaggcagg
    30961 atctcttaac gaagttgaat tccttcgctt tgactttacc cacttccaag cagtaactgc
    31021 tgaggaactt cgtgcgattg aacagcaagt taacgagaaa atttgggaag cacttgaagt
    31081 gaagacagtt gaaacagata ttgacactgc taaagaaatg ggagctatgg ccctctttgg
    31141 tgagaaatac ggcaaggaag ttcgtgtcgt gactatcggt gactactcta ttgaactttg
    31201 tggtggtact catgttggca acacttctga gattggtctc ttcaaaattg tcaaagaaga
    31261 aggaatcggt tcaggaactc gccgtatctt ggcagtgact ggtaaggaag cctttgaagc
    31321 ctatcgtgaa caagaggatg ctcttaaagc tgtcgcagca accttgaaag cacctcaagt
    31381 caaggaagta cctcacaagg tagaaggact tcaagaacaa cttcgtcaac ttcaaaaaga
    31441 aaatgctgag ttgaaagaaa aagccgcagc tgcagccgca ggcgatatct tcaaagatgc
    31501 taaggaagtc aacggtcatc gttacattgc tagtcaagtg tctgtatccg atgccggtgc
    31561 ccttcgtact tttgcagata actggaaaca aaaagactac tctgatcttc ttgtcctagt
    31621 tgccgctatc ggtgacaaag tcaatgtcct tgtagcaagc aagacaaaag accttcatgc
    31681 aggaaacctt gtcaaagaat tagcaccaat catcgatgga cgtggtggtg gtaaaccaga
    31741 catggccatg gcaggaggaa gcaaccaagc taagatccaa gaattgttgg atgcagtagc
    31801 aggtaaattg taatcaagta aagatctatc catttgggta ggtctttttg tgaatacaaa
    31861 aaagccaaat ccgcttggac ctagcttggt tattatgagc tattagatgg tattgactgc
    31921 ccagacactt acggatctag ctgagacagg gaattgtcca taaccttcct catcaattgt
    31981 aacttgacct tggtggttac caagtaaatc tacaaaggtt tgattagtcc attcttgacc
    32041 gacaaacatt gacttgctgt tttcttggtc atttgagata aggactgcga ttggggattg
    32101 attttcagca cctgaacgta cccaaccgat acagttagca tggtcaaagt agtcattttg
    32161 ttctccatag gccaagtctt ttcggatggc taggaggcgg tcaaggattt ctttgaaatc
    32221 ttcttgagca tactggcctg aaatcccata gtagtctccg taaaagacac atggaaggcc
    32281 gtcttggcgt aacaaaatga gggcataggc tgctggcttg aaccattctt caacggtaga
    32341 ctcaagggct tgtcctcgtt gggtatcgtg gttgtcgaca aaagtcacag ccttgtcagg
    32401 cttgagttca accaggctat ctgtaaaaat gccacgaagg tcatagtttg cgccagcttg
    32461 actggcttca aagagattct ggtggagacg aacatcgaca aggtcaaagt gttcttccgt
    32521 tttttcgaga tagtccagat tggcttcctt gtctgggttc caaaattcac caaaaacata
    32581 gaaatcgtca ccgtattttt ccttcatatc gcggatgaag ttgcgcataa agaaagagtc
    32641 aatatgctta acggcatcca aacggaaacc agctacacca gtcgtttcca tgaaccaatc
    32701 agcccagtca tagatgtttt ggatgacttc aggatgttta aagtctaggt cggcatacat
    32761 gaggtagtcg tagtttccgt tttcgttatc gaccaattcc tcgttagccc agcccttgtt
    32821 gtccccttgg atcagataaa tcccagattt actgcgtttg gcatcgtagt ctgtaccggt
    32881 gaagtggtac caatgccagt ggaagccatt ataggtatct tggcgaccat cgaaggtaaa
    32941 actagtccag ccattgatgg tgaagggttc tccaagttca actgtacggt ctacaggatc
    33001 cacttcaata acctgaaagg cttccctgtg atcggcagca gccttgtggt tgagaactac
    33061 atcggctatt ggttgaattc cctgcgcttt aagggcttga atggcttgaa gatagtcttc
    33121 tttgaaacca tacttggtgc ggacagtccc tttttggttg aactctccta ggtcaaataa
    33181 gtcatagacc ccataaccta catctttttc attggttgcc ttaaaagctg gtggcatcca
    33241 gacgtgactg atccccagat gagctaggtg tggagcattt tcagccagac gcgtccagtg
    33301 ttgaccgtcg tggggcagat accattcaaa gtattgcatg agtgtttgat tttgcatgat
    33361 gtttcctctt acttgtcaat cttgttcttt attctatcat aaagtatcta ttagcgcaaa
    33421 cgtttgcgta agatagaagg aaactatttt aactacttaa aataaagtat tgattttttt
    33481 gtatcaaagt gctagtatag cgatgtataa tacagataaa gataggagtt gtcccatgat
    33541 tgaatttcaa aatgttagta agttgtatgg tgataaagag gccttgagta atctcaattt
    33601 gcagattgaa aatggagaga ttatgggctt gattggtcat aatggggctg gaaaatcgac
    33661 caccataaaa tccctagtca gtatcatttc acccagcagt ggtcgtattt tggtagatgg
    33721 tcaggattta tcggaaaatc gcttggcgat caaacgaaag attggctacg tagcagactc
    33781 gcctgactta tttttacgtt taacggccaa tgaattttgg gaattgattg cttcatccta
    33841 tgatttgagc agctctgact tggaagctag tttagctagg ttattgaacg tttttgattt
    33901 tgctgaaaat cgttatcagg ttattgaaac cctttctcat ggaatgcgtc agaaagtttt
    33961 tgtcatcggg gcactcttgt ctgatcttga tatttgggtc ttggacgaac ccttgactgg
    34021 tttggatccc caggctgcct ttgatttgaa gcaaatgatg aaggagcatg cgcaaaaagg
    34081 taagacagtt ttgttttcaa ctcatgtcct agaggtagct gagcaagtct gtgatcggat
    34141 tgctattttg aaaaaggggc atttgattta ttgtggtaag gtagaggact tgagaagaga
    34201 caacccagac cagtctttgg aaagtatcta ccttagcctt gctggtagaa aagaggaggt
    34261 ttcagatgcg tctcaaggtc attaaaaaat tagttgatat caatatcctc tattcatctc
    34321 aagaagctaa tctggctaac ctacgaaaga agcaagctaa aaatactgga aaaaaagtaa
    34381 atgtttccgc tagagtccta agttcttaca ttttttccag tctcttgatg ctcatctttt
    34441 ttagtactat agccattcat tttccttttg aggaaatgcc tgtttatttt agttttgtgg
    34501 ttgctatttt actagtgatt gccttttcaa cttctttaac tgcattttac aatgtctttt
    34561 atgagagtaa ggacctggcc tcctataggc cctatgcctt taaagaatca gagattataa
    34621 ttgctaaagg actgtctgtc cttttgccag ctctaactgg aattgtacca atcctagctt
    34681 attttctagc cctctatatt agtctagctc cttctctttg gctgggtttg cctttgatgc
    34741 tactgtcctt gaccttatta tttgtctctg tggctctagt gatggtagtg gcagtgcatt
    34801 tcttggctca aactacggcc ttcagaaagt atcagtctat tttttcgaat gtgatgattg
    34861 ggataggagt tctcatacct ttaatatttg tcttctttct acagtcgact tttggaagta
    34921 ttgttgacaa agttagagac attccatttc tcctttatcc tcttcatatc ttttacaaaa
    34981 tagcagtgga gcctttttcg acagaagcct tagtgggtct gctcgcttgg ataggactaa
    35041 ctctcttcct gctttatctg accaaaaaga aggtccttcc tcgtttttat gacgtgatcc
    35101 tgcttaatag tgaggagaag gtcaaaaaag aacgtcgcag caaggagaga atttcaacca
    35161 ctaaaaaggg atttttccgc atggttttac gctaccacct caccctcttg ggacagggta
    35221 ctggcgtggt tacagtgctt tttacaagtg ctttccttcc ttatctcatg atgatcggtc
    35281 tgatttccaa aatccgagat tctcagatag ttccagacat tcatcctcca tactggttac
    35341 ccttgttttt tatagctctc tttatagcag ttgtcaataa caatatcacc agcctgcatt
    35401 caattgcctt gtccttggag agggaaaatg ttgattttct gaagagttta ccctttgact
    35461 ttgctcgtta tgtgaaagtg aaattttgga ttatatatgc cgtccagtcc tttttaccaa
    35521 ttctgacttt acttggtctt tctctatatc taggcttgcc catcatttcg atgatttacc
    35581 ttattatggc atggatcctt gccagtgtca tcctttcttg ccaccattac tttaaggacg
    35641 ttaaaaatct gtcaatcaat tggagtagca ttacggacct ggtgaatcgt tcaaatggca
    35701 tagtcgccat agttttattg tttatttata gtgcaatcct gatggccctt gtaattggga
    35761 gcatattctt ggttcagtct ctctccacta tccttgccat cagcttggga gtaggagctc
    35821 ttatcctcct gcttgctctt gctatttttg gctatcatta ttacctgtca cgcatattgg
    35881 cagaaataga aaaaagatga gctggttggt cgcttgcttg ataaattcaa ttcgacaaac
    35941 gcttttatag tttgttagaa tgtagttgta aatgattaag attggtaatc aaaaatatag
    36001 ggaggaatgt tttgaataag aaatataaca tagttctatt tttgttgttt atagtttatt
    36061 tattgggata tttttcaatt tcaaaaacgt taattcctat aatgtgtgtg atccaagtat
    36121 ttttgataga acatatattt agagttcgaa atagaatgat gcaaataggt gaaattataa
    36181 ttattgttgc ttctataata ttatttattg atagtatatt gagtttgtaa caaaaggctt
    36241 ctctataaaa tcaaattttc cagatatcta ctgaagctga tttatttctt catctttctt
    36301 ctactacctt tgtcactaaa tttgacagac acatattgct gatacaacct ctctacctat
    36361 aatctcacag ttaacctgag gctagtttca tagtttgcta tttgattttc gttgataata
    36421 aaaaatgctc ctccaagacg gaagagcatt ttttattgtg attcttacac atttacttcc
    36481 tagttttgca ggaagtctag tataccattt tttaaaagaa gagtaaaaaa gcaagggtag
    36541 tttctttttt gagaattgtc taaaagtttg atataatggt agttatgaat agaataagag
    36601 tcagcaaaag ggttgaaaag aagcttgcta aggggctagt tttactagaa gccagtgatc
    36661 ttgagaatgt caatcttaag gatcaggaag tagaggtgca gggtcaggaa ggaaactttc
    36721 ttgggactgc ctacctttct cagcaaaaca agggcttggg ctggtttgtc agcaaagaca
    36781 aggtgacctt caatcaaact ttctttgaaa cgttgtttag aaaagccaaa gaaaagagaa
    36841 acgcctacta tcaagatgat ttgacaactg cctttcgtct ctttaatcaa gagggagatg
    36901 gctttggggg tctgacagtg gacctttatg gcgactacgc cgtcttttct tggtataact
    36961 cttatgttta tcagattcgt cagactatat cagaagcctt tagacaggtt ttccctgagg
    37021 ttttaggggc ttatgagaaa atccgcttta agggttttga ctatgaatct gcccatgttt
    37081 atggtcaaga agcacctgac tttttcactg ttttagaaaa tggtgtcctg tatcaagtct
    37141 ttatgaatga tggcttgatg acaggaattt tcctagacca gcatgaggtt cgcggtagtt
    37201 tagttgacgg cttgactatg ggtaaatcct tgctcaatat gttttcctac acagcggctt
    37261 tttcagtagc tgcggccatg ggaggagcta gccagacaac ttctgttgat ctagccaaac
    37321 gttcacgaga attgtctcaa gcgcattttc aggcaaatgg gctcagcaca gacgagcatc
    37381 gttttatagt catggatgtc tttgagtatt tcaaatatgc caaacgcaaa gacttgacct
    37441 acgatgtgat tgtcctagat ccgcctagct ttgctcggaa taaaaaacaa actttctctg
    37501 tggccaagga ttatcacaag ttgatttccc agagtcttga gattttaaat ccgggaggga
    37561 ttatcattgc cagtaccaat gctgccaatg tttcccgcca gaaatttaca gaacaaattg
    37621 ataaaggttt tgcaggaaga agttaccaga ttttaaacaa atatggtctt ccagcagatt
    37681 ttgcctataa taaaaaagat gaaagtagta attacctcaa ggtgattagt atgaaggtta
    37741 gtaaatgaaa ttaatcgttt cagtaatgcc aagaagttta gaggaggctc aggctctgga
    37801 tgccacgagg tatctggatg ccgacatcat tgaatggcgt gccgactatc tgcctaaaga
    37861 agcgattttg caggtagctc cagccatttt tgaaaaattc gcaggccgtg agttggtttt
    37921 cacgctacga actcgctccg aaggtggaga aatcgacctt tctccagaag agtatatcca
    37981 tctaatcaag gaagttgcgc aactctatca accagactat attgattttg agtactatag
    38041 ctacaaggat gtttttgagg aaatgctgga cttcccaaat cttgttttga gttaccataa
    38101 tttccaagaa acacctgaga acatgatgga aatcttgtca gagttgacga tcctaaatcc
    38161 aaaacttgtt aaggttgcgg tgatggctca cacggagcag gatgtcttag acttgatgaa
    38221 ctatacacga ggctttaaaa ccctcaatcc tgaacaggaa tatgtgacca tttctatggg
    38281 taaggtgggc aaggtctctc gtatcactgc ggatgtgact ggttcgagtt ggtcttttgc
    38341 cagtctggat gaggttagtg cccctgggca gatttctcta gctagcatga aaaaaatcag
    38401 ggagattttg gatgaagctt gatggctata cacgtttagc tgcagttgtt gccaatccta
    38461 ttaagcattc tatttctccc ttcatccaca atagagcctt tgaggcgaca gataccaacg
    38521 gtgcttatgt ggcttgggag attgaagcga gtgacttggt agaaacagtg gccaatattc
    38581 gtcgctacca gatgtttggc atcaatctgt ccatgcccta taaggagcag gtgattcctt
    38641 atttggataa gctgagcgat gaagcgcgct tgattggtgc ggttaatacg gttgtcaatg
    38701 agaatggcaa tttaattgga tataatacag atggcaaggg attttttaag tgcttgcctt
    38761 cttttacaat ttcaggtaaa aagatgaccc tgctgggtgc aggtggtgcg gctaaatcaa
    38821 tcttggcaca ggctattttg gatggcgtca gtcagatttc ggtctttgtt cgttccgttt
    38881 ctatggaaaa aacaagacct tacctagaca agttacagga gcagacaggc tttaaagtgg
    38941 atttgtgtgc tttagaatat gtttctgaac tgcaagcaag gattgccgag tcggatttgc
    39001 tagttaatgc caccagtgtg ggcatggatg gccaatcctc tcctgttcct gaaaacatag
    39061 tcttaccaga aactctctta gttgcagata tcatatacca accctttgaa acaccgtttt
    39121 tgaaatgggc tagaagtcag ggcaatccag cagtcaatgg tctgggaatg ttactctatc
    39181 aagctgcaga agcttttcaa ctgtggacag gcaaggaaat gccgacagaa gagatttggc
    39241 agtctttaac agaaaaatac caataaagaa agggagactc tcctatgaaa atcagaatcg
    39301 atattcctca tcatccttat gatattcaga ttgaaaaagg ttgtatggcc caggctggtc
    39361 agtggttgcg agaactctgg caaccgcaaa aggtagtcat tgtgacagat aaccatgtag
    39421 cctctctcta tgcagagaag gtcaagctca gcctagaaga tgctggtttt caggtagctg
    39481 tttttgattt cttagaaggt gaagaaagaa agaatttaac tactgttcag aaagtctatg
    39541 aatttctagt caagcaaggt ctgactcgta gcgatggaat cgttgctctt ggtggtggcg
    39601 ttgttgggga cctggctggc tttgtagcct ctacctatat gcggggtatt cactttgttc
    39661 agattccgac tagtttgaca gctcaggttg attcttctat cggtggaaag acaggcgtta
    39721 acacaccatt tgctaaaaat atggtgggga cctttgccca accagatggg gttctgattg
    39781 atccacttgt tcttgaaacc ctcggaaaaa gagagttgat tgaagggatg ggtgaagtta
    39841 tcaagtatgg cttgattgag gatccagaac tgtgggctct cttgacggga ctgaatggtt
    39901 ctgttgagag tattttggaa catgcagaga ccttgattga acattcttgt caggtgaagc
    39961 gcaagatggt ggttgaagat gagttggaca atggtattcg tctttacctc aattttggcc
    40021 acactattgg ccatgccatc gaagcgactg ccggttatgg caaggtcatg catggagagg
    40081 ctgttgccat gggaatggta cagatttcca aggttgctga ggaaaaaggc ctcatgccag
    40141 ctggcattac ccaatctatc acagagatgt gtcagaaatt cggcttgcct gttgactatg
    40201 aaaattggga agttgacaag ctttatcagg ctcttactca tgacaagaaa gcgcgtggta
    40261 acaccttgaa attggtcttg gtgccagagc ttggttcagc gatcattcac ccagtttctc
    40321 tggaagagat gaaagactac ttggtaaaat aaggagagtg tatgagatat ttaactgcag
    40381 gagaatcaca cggcccccgt ctaacagcta ttattgaggg aatcccagct ggacttccat
    40441 tgacagctga ggatatcaat gaggacctta gacgccgtca gggtggctac ggtcgtggtg
    40501 gtcgtatgaa gattgagagt gaccaggttg tctttacttc gggcgttcgc cacgggaaga
    40561 cgacaggggc gcctattact atggatgtca tcaataagga ccaccagaaa tggctggaca
    40621 tcatgtctgc ggaggacatt gaagaccgcc ttaaaagcaa gcggaaaatt actcatcctc
    40681 gcccaggtca tgccgatttg gttgggggga ttaagtaccg ttttgatgat ttgcgaaatt
    40741 ctttggagcg ttcatcagct cgtgaaacca ccatgcgggt ggcagttggt gcagtagcca
    40801 aacgcctctt ggctgagctg gatatggaga ttgccaacca tgtcgtggtc tttggtggta
    40861 aggaaataga tgttcctgaa aatctgacag tcgctgaaat taagcaaaga gctgcccagt
    40921 ctgaagtttc tattgtcaac caagaacgag aacaggaaat caaggactat attgaccaaa
    40981 tcaaacgtga tggtgatacc atcggtgggg ttgtggagac agtcgtcgga ggcgttccag
    41041 ttggtcttgg ttcctatgtc caatgggata gaaaattgga tgcaagattg gctcaagctg
    41101 ttgtctctat caatgccttt aaaggggtgg aatttggtct tggctttgag gctggttatc
    41161 gtaaaggcag ccaagttatg gatgaaattc tctggtctaa agaagacggt tatactcgcc
    41221 gtaccaataa tctaggtggt tttgaaggtg gtatgactaa tgggcaaccc atcgttgttc
    41281 gtggggtcat gaaacccatt cctactcttt ataaacctct tatgagtgtg gatatcgaaa
    41341 cccacgaacc ttacaaggca accgtggaga gaagtgatcc gactgctctt ccagctgcag
    41401 gaatggtcat ggaagcagtt gtagcaacgg ttctggcgca agaaatcctc gaaaaattct
    41461 catcagataa tcttgaggaa ctaaaagaag cggtagccaa acaccgagac tatacaaaga
    41521 actattaagg agttcctatg gcaaaaacaa tctatatcgc aggtcttggg ttgattggag
    41581 cctctatggc acttggtatc aaacgcgatc atccagatta tgaaatttta ggttataatc
    41641 gtagtcaagc ttcgagagat atcgccttga aagaaggtat gattgaccgt gcaacggatg
    41701 attttgctag ttttgctcct ttggcagatg tcattatcct cagcttgcca atcaaacaaa
    41761 ctattgcttt cattaaggag ttggccaatt tggatttgcg agaaggcgtt attatttcag
    41821 atgctggttc gaccaagtca accattgtgg atgcggcgga gcagtatttg gctggcaagt
    41881 ctgttcgctt tgtcggggcc catcccatgg ctggtagtca caagacaggg gctgcttcgg
    41941 cagatgtcaa tctttttgaa aatgcctatt atatctttac accttcaagc ctgacaagtc
    42001 aggacacgct taaggaaatg aaggatctgc tttcaggtct tcatgctcgt tttatcgaga
    42061 ttgatgccaa ggagcatgat cgtgtcactt ctcagattag ccatttccct catattttgg
    42121 cttctagtct catggagcag actgcggtct atgctcaaga gcatgagatg gcaaggcgct
    42181 ttgcggcagg tggttttcga gatatgaccc gaattgcgga aagcgagcca ggaatgtgga
    42241 cctccattct cttgtccaat agcgagacca ttctggatag aattgaggat ttcaaggaac
    42301 gtttggaagt gattggtcag gccattagta agggagatga agagcaaatt tggaactttt
    42361 ttaaccaagc gcgtgaacaa cgccagacca tggaaatcca taagcgtggc ggtgtggata
    42421 gctcttacga cctctatgtt gacgttcctg atgaagaaga tgtcatcctg cggattttgg
    42481 aactgctacg tggaacttcc ttggttaaca tccacatcaa cgaggaaaat cgtgaggata
    42541 ttcacggaat cctacaaatt tcatttaaaa atgctcaaga cttggaaaga gctgagcatc
    42601 tcataacaga aaataccgac tatacagtcg tcatcaaata aggagaagaa catgtcaaat
    42661 atttacgata gtgcaaacga actcagtcgc ggtctacgcg gattaccaga atacaaggct
    42721 gttaaagcag ctaaagatgc gattgcagca gatgctgagg caagcaaaat ctttacagaa
    42781 tatcttgcct ttcaagagga aattcaaaaa ctagcacaga caggtcaaat gccagatgcg
    42841 tccttccaag ctaagatgga aggctttggt aaacagattc aaggaaatag cctcttgtca
    42901 gaattcttta ccaagcaaca acaattggcc atttaccttt ctgacattga aaaaattgtt
    42961 tttgaaccag tttcagaatt gctgaaataa gaaaagaact atcctaaagt caggaatttt
    43021 taaggaattt ctggcttttt atgataaaat aggtaaaagt attgcaagta tgaggtccag
    43081 tatgaaacta aaaacaaaca ttcgccattt acatggtagt atccgcgtcc caggtgacaa
    43141 gtctatcagc caccgttcca ttatctttgg aagtttggct gagggtgaga ccaaggttta
    43201 tgatattctg cgaggtgaag acgttctttc gaccatgcag gtttttcgtg accttggtgt
    43261 tgaaattgag gataaagatg gggttattac cattcaaggt gtaggcatgg ctggcttaaa
    43321 agcgccgcaa aatgccctta atatgggaaa ttctggcacc tcgattcgcc tgatttcagg
    43381 tgtccttgct ggtgcagatt tcgaagtaga gatgtttgga gatgatagtc tttccaaacg
    43441 tcctatggac cgtgtgaccc ttccactgaa aaaaatgggc gtcagcattt caggacaaac
    43501 tgaacgagac ttgcctcccc ttcgcttaaa agggacgaaa aacctaagac ctattcatta
    43561 tgagttgcca attgcctctg cccaagtcaa gtcagccttg atgtttgcag ccttacaagc
    43621 taagggggag tcagttatta tcgaaaaaga gtacacccgt aatcatactg aagatatgtt
    43681 gcaacaattt ggtggtcatt taagtgtgga cggtaagaaa atcacagtcc aagggccaca
    43741 aaaattgaca ggacagaagg tggtcgtacc aggagatatt tccagtgcag ccttttggtt
    43801 agtcgcaggt ttgattgctc caaattctcg tctagtgctg cagaatgtgg ggatcaacga
    43861 aactcgcacc ggtattattg atgtcattcg tgccatgggt ggaaaattgg aaataactga
    43921 aatcgatcca gtcgctaaat ctgcaacctt gattgttgag tcttctgact tgaaaggaac
    43981 agagatttgt ggcgctttga ttccacgttt gattgatgaa ttgcctatta ttgccctact
    44041 tgcgacccaa gcccaagatg taacagttat caaggatgct gaggagctca aggtcaagga
    44101 aacagaccgt attcaggttg tggcagacgc cttaaatagt atgggagcag atattactcc
    44161 tacggcagat gggatgatta tcaaaggaaa atcagctctt cacggtgcta gagtcaatac
    44221 gtttggtgac caccgtatcg gcatgatgac agctatcgca gccctattgg ttgcaggtgg
    44281 agaggtggag cttgaccgtg cagaagccat caataccagc tatcctagtt tctttgatga
    44341 tttggagagc ttgattcatg gctaaggtgt tattagggtt tatgggggct ggaaaatcga
    44401 ctattgcaag aggcttggac cctaattacc ttgatatgga tgctctgatt gagaagcgcc
    44461 taggtatgtc cattgcgaat tttttcgctg aaaagggaga agcagccttt cgtcaggtag
    44521 aatcagaagt cctagctgat ttactacaaa cagaccaagt cgtgtcaact ggaggaggag
    44581 tggttatttc tcagagaaat cgtgacttac tcaagactaa tacagataac atctacctga
    44641 aagcagattt tgaaaccctc taccaacgta tcgcagctga taaggacaat cagcgaccgc
    44701 tttttctaaa taatagcaag gaagaactag tagctatttt tcaagaaaga caggcttggt
    44761 atgaggaagt ggctagtcgg gttttggatg tgaccaagct aagcccagag gaaattatag
    44821 aggaactaag atgaaaattg cttatctagg tcccaaagga tcattttcac accacgttgt
    44881 gcagacagct tttcctcatg aggaattgca ggcttttgcc aacattacag atgtcatcaa
    44941 ggcttatgag caaggattag tggactattc tgtggtgcca gttgaaaatt ctattgaggg
    45001 tagtgttcat gaaaccttgg actatctttt tcatcaggct cacatccaag cagtagcaga
    45061 aatcgttcag cctattcatc agcagttgat ggtggttcca ggccatacta agattgaaaa
    45121 gattttttca catccacagg ccttggctca aggaaagaaa ttcatcgatg aacaatatcc
    45181 agaggctcaa atcgaggtaa cagctagtac agcttatgcg gcccgtttta tttccgaaca
    45241 tccagaccag ccctttgcag cagttgcacc tagaagttct gctgaagaat atggattgga
    45301 actgattgcc gaggatattc aggaaatgga agccaatttc acacgtttct gggttctagg
    45361 agctgaaaag cctagtattc ccttgcaagc acaaactgaa aagatgagtt tggccttgac
    45421 attacctgac aaccttccag gtgcacttta taaggccctg tcgacctttg cttggcgagg
    45481 aattgacttg acaaaaattg aaagtcgtcc actcaagaca gcactgggtg aatacttttt
    45541 cattatcgat gtggattata ccgataagga cttggtccac tttgcccaaa aagaattaga
    45601 agcgattgga atccagtata aaattctggg tgcctatcct atttatccaa tatcagacca
    45661 tggaaaggag agaagatgag taaagaaaat cccttaagtc atcatgagca gttgcgttat
    45721 gattatttgc taaaaaatat tcactatctc aatgagagag aaaaaaatga gtttgcctat
    45781 ttgcaagaaa agctaactct tgctagggga aatagtagct ctagcttgga acaagaaaga
    45841 gaagagcagg ttgacttacc aagctatgcg aaccggagtc gctcacaatc caaatcacaa
    45901 gcactctctt tccctccaaa aaagaaaaga cggaagctcc gtcttaaacg aatttttatg
    45961 gtgatttttt cacttttggt ctgtgtggct ttggccatgg tattcatgtt tttgcgtggt
    46021 taccaagatg ctagcgcaaa gaaaactgct gatgcccggg cagctcaagt agaagtcttt
    46081 aatggtcagg acactagaga tggagttaat attttaatca tgggtactga tggtcgaatc
    46141 ggccagaaca gtgttgagac acgaactgac tctattatgg tattaaatgt cgggggctca
    46201 gataagaaaa tgaagctagt cagtttcatg cgtgataatt tggtctatat agatggttat
    46261 agtcaagtga ttaatggtag aaaacagaca gataacaagt taaacgtagc ctacgagtta
    46321 ggagaacaag aggggcaaaa aggggcagaa atggttcgcc aagtcttgaa agataatttt
    46381 gacttggaca ttaagtacta cgccttggtt gatttccagg cctttgccac agcgattgac
    46441 acgcttttcc ctgatggggt gacgattgat gctcaatttt caacattgaa tgggcgtccc
    46501 ctaacagaag ctacagtcgg agatgattta tatgctaccg agactgagtc tccaacccaa
    46561 accattaaag tcggaaaaca gcagatgaat ggctcgaccc tgctcaatta tgctcgtttc
    46621 cgtgatgatg atgaggcgga ttacggccgt acaaaaagac agcaacaagt tttaacagca
    46681 attttagagc aaattaaaga tcccactaaa cttttcactg gttctgaagc tcttggaaag
    46741 gtttttgcta tgacctcaac caacgtacct tatactttcc tcctaacaaa cggtttatct
    46801 gttttggatg gagcaaaaaa tggtattgaa aaattgacga ttccagaact gggtgactgg
    46861 gtagatgcct atgatgttta tggaggcttg ggcttgctgg ttgatcaaaa caaatatcaa
    46921 accaagctcg ctcaaatggg cttgagataa gataatactc aatgaaaatc aaagtgcaaa
    46981 ctaggaagct agccgcaggc tgctaaaaac acagttttga ggttgtagat aagactgacg
    47041 aagtcagtta cctataccta cggcaaggtg aagctgacgt ggtttgaaga gattttcgaa
    47101 gagtataaga cagaaaaatc aaagtttgat agctatttgg atagcagtca ggctttgatt
    47161 ttttacagtc tgcaatagat tttcacccct tgtttgtggt ataatgaaac gatacgatag
    47221 aaagaataat gaacaatatg actgatttaa aagcaattca ggctcgtagt ctggagatgg
    47281 ctgaatattt tgtggccttt tgtaaagaac atgatttgct ctgttatctc tgtggcggag
    47341 gcgctattgg tgcccttcga aacaagggat ttattccttg ggacgacgac ctagactttt
    47401 ttatgcctcg taaagattat gagaaattag cagaattatg gcctcgttat gcagatgaac
    47461 gttatttctt gtcaaagagt cacaaggatt ttgttgatcg taatcttttt attaccattc
    47521 gtgacaagaa aaccacctgt atcaagcctt atcagcagga tttggatttg ccacatggtc
    47581 tggccttgga tgttttgcct ttggattatt atccgaaaaa tccagctgag cggaaaaaac
    47641 aggttcgttg ggccttgatt tattcactct tttgtgcgca aactattcca gaaaagcatg
    47701 gtgctctcat gaaatgggga agttgcattt tactgggttt gactccaaaa tctctccgtt
    47761 atcgcatctg gaaaaaagct gagaaagaaa tgactaagta tgatttggct gattgtgatg
    47821 gcattacaga attatgctca ggtcctggct acatgagaaa caagtaccca atcacatctt
    47881 ttgaagacaa tcttttcttg ccatttgaag gaacagagat gcctattcca atcggctatg
    47941 atgtctatct cagaactgct tttggggatt atatgacgcc tccaccagca gacaagcagg
    48001 taccgcatca tgatgctgtc atcgctgata tggataagtc ttatacagaa tacaagggag
    48061 aatatggtgg ctaagaaaaa aatcttattt tttatgtggt ctttttctct tggaggtggt
    48121 gcagagaaga ttctatcaac tattgtttca aatctggatc cagaaaagta tgatattgat
    48181 attcttgaaa tggagcactt tgacaaggga tatgaatctg ttccaaagca tgtacgcatt
    48241 ttaaaatccc ttcaagatta tcgccaaacc agatggttac gagctttttt gtggagaatg
    48301 agaatttatt ttccaagact gactcgtcgt ttgcttgtaa aagatgatta tgatgttgaa
    48361 gtttctttta ccattatgaa tccaccactg ttgttctcta aaagaagaga agtcaagaag
    48421 atatcttgga ttcatggaag tattgaagaa cttcttaagg atagctctaa aagagaatca
    48481 catagaagcc agttggatgc tgcgaataca attgtaggga tttcaaaaaa gaccagcaat
    48541 tctatcaagg aagtttatcc agattatgct tctaaattac agacaatcta caatggatat
    48601 gattttcaga ctattctaga aaaatctcaa gagaagatcg atatcgagat tgctcctcaa
    48661 agtatctgta ctatcggacg gattgaggaa aataagggtt ctgaccgtgt agtggaagtg
    48721 atacgattat tataccaaga gggaaaaaac tatcatctct attttatcgg ggctggtgat
    48781 atggaagagg aactgaaaaa acgagtcaaa gagtatggga ttgaggacta tgtacatttc
    48841 cttggttatc aaaaaaatcc ttatcagtat ctatctcaga cgaaagttct cttgtctatg
    48901 tctaaacaag aaggttttcc tggagtgtat gtggaggcct tgagtctggg actccctttt
    48961 atctctacgg acgttggagg ggctgaggaa ttatcccaag aaggacgatt tggacaaatc
    49021 attgagagca atcaagaggc agctcaggcg attactaatt acatgacttc tgcctcaaac
    49081 tttgatgtcg atgaggctag ccaattcatt caacaattta caattacaaa acaaatcgaa
    49141 caagtagaaa aactattaga ggagtagcat ggaaactgca ttaattagtg tgattgtgcc
    49201 agtctataat gtggcgcagt acctagaaaa atcgatagct tccattcaga agcagaccta
    49261 tcaaaatctg gaaattattc ttgttgatga tggtgcaaca gatgaaagtg gtcgcttgtg
    49321 tgattcaatc gctgaacaag atgacagggt gtcagtgctt cataaaaaga acgaaggatt
    49381 gtcgcaagca cgaaatgatg ggatgaagca ggctcacggg gattatctga tttttattga
    49441 ctcagatgat tatatccatc cagaaatgat tcagagctta tatgagcaat tagttcaaga
    49501 agatgcggat gtttcgagct gtggtgtcat gaatgtctat gctaatgatg aaagcccaca
    49561 gtcagccaat caggatgact attttgtctg tgattctcaa acatttctaa aggaatacct
    49621 cataggtgaa aaaatacctg ggacgatttg caataagcta atcaagagac agattgcaac
    49681 tgacctatcc tttcctaagg ggttgattta cgaagatgcc tattaccatt ttgatttaat
    49741 caagttggcc aagaagtatg tggttaatac taaaccctat tattactatt tccatagagg
    49801 ggatagtatt acgaccaagc cctatgcaga gaaggattta gcctatattg atatctacca
    49861 aaagttttat aatgaagttg tgaaaaacta tcctgacttg aaagaggtcg cttttttcag
    49921 attggcctat gcccacttct ttattctgga taagatgttg ctagatgatc agtataaaca
    49981 gtttgaagcc tattctcaga ttcatcgttt tttaaaaggc catgcctttg ctatttctag
    50041 gaattcaatt ttccgtaagg ggagaagaat tagtgctttg gccctattca taaatatttc
    50101 cttataccga ttcttattac tgaaaaatat tgaaaaatct aaaaaattac attagaacgg
    50161 gggtgaccaa gtgattgatg ggaaacgatt attatttagt ttgaccatag tcagttatgc
    50221 cttgacgcta gtaagtggaa ttgtgtatct gtttaataat aataatgtta gcttactttc
    50281 tactttattg ttcttgttgg ttagtagctt gattgcttgt tggaatgata tcaagtatta
    50341 cttaatccat tttattttct atttaaccat ttttgtattt ctggtatcaa gaccgaccat
    50401 tgattatttt agggatggtg ctttggatac ctatcatcca atagcctatc gttttgcctt
    50461 tatagttgtc atggtttcga ttctgggctt gacaacagga ggcgtcctgg ctcgttactt
    50521 catagctagg aagaaaataa aagtagcaaa tataggaaat tctctaaaag aggtttatat
    50581 caagcggtta cgctttgtat cactaggagt ttttcttcta acttatcctt tctatttcat
    50641 tcggttattt gaacggctct tgtatcgttt gcagacttcc tactatgcct actatgcaaa
    50701 ttttgaaagt aaactgcctt attttaccta cattttgtct acctttacgg tctatgcaat
    50761 gtgtatgtat ctggcaacca agccaaagaa attgcaggcc acagcagtgc ttgtctcctt
    50821 tattgcagct aacactattc atttggcaat tgggacacga aatcccttta ttttaagtat
    50881 tttatttgct tttgtttatt actttatgcg ggagcaagct gaaaaaggaa aatggattgg
    50941 gtttaaagaa aagttagcga tttttgtagg ttctcctatt ctcatgttag cgatgggagt
    51001 actcaattat gtacgggata atgtccaagt ttcccataca ggtttctggg atatcttact
    51061 tgactttatc tataaacaag ggactagttt tggtgttctg gctcgaggtt ttctatttaa
    51121 cagtagcctc ccttaccgag atttccgtaa ttttactttt ggtcctgttc ttgattattt
    51181 tgcaaggggg agtttgggag ccattttcgg aggaaaagcc tttgaacata caaccaatag
    51241 tgtggaacta gctattgata gtaatagtta tgcccacaat ctatcctatc ttgtcttgaa
    51301 caaggaatac ttgaaagggc atggtatcgg aagtagttat atcatggagt tgtataccga
    51361 ctatggtatg attggagtct ttctgcttag tttcttactc ggcatattat ttatagccat
    51421 gctgcaagta gcctatcgct caaggacaat cctatttgct ttatccctac tcatcttgaa
    51481 taatctattc tttatgccaa gaagcagctt ttcagaaagt ttcttcaatt tatttacaat
    51541 gcaattctgg ggaattgttc ttgtgattat atttgtagca aaaatgctta caaaggaaaa
    51601 ctagtatcta ctaaacaaag gagaaaaaaa tcatgtttga acattattca gtagctgatt
    51661 tgtttgcaaa tctttataaa aaacgaaaag caaatatttt agctctcatc gctttatttg
    51721 ctctcattgc tgtaccattt acaattaaag cagttaggaa taaaaacact gtcaaagata
    51781 caacaagtta ttcaacttat cttatctata aaatcactcc tccagaagat tcggccaaaa
    51841 cgattttgaa tcatcaaatt ggtggttata gtgattttta tgggaaattg attgatggta
    51901 atttgaatgg agcttatctt ttcaatgatg tagaacccag tgagttgaaa aaaattgcca
    51961 gtgaattaga cacgacagaa acaaccttga aaaattcaac gaatgactat tggtggaaaa
    52021 aattgaccgt ctactatatg attgacgatg caggggttgg tgtgaaaatt ttgacatcaa
    52081 gtaaagatgc caatgacttg ttagagaaaa aaattgatgg gttgattgag aaatttaaac
    52141 atgcctatgc aaatgtaaaa attgaaaaac tggaaaccat caactctaaa gaattgaacg
    52201 caaatggtga aacagcgctt ggcttaaatg tgaaaaatct gattcttcgt ttagttgtta
    52261 ttggagtggt ttgtgtgatt ttggttgtga tgggaaatgt gttagtttat ctctttaatc
    52321 caacaatcaa tagagtaggt gatttttctc agtatcaaat tgattttgta acagagatta
    52381 caacaattgc taacctagca gatgttttgt catacaaaaa tactggacag gaattgacca
    52441 tcgttagctc aaataaagct atcctagata aattgaaaca gagtcaagaa gctttaaaag
    52501 gaatgcattt tgtagattta caggatgtat catctctttt ggaaagagat acagtccttc
    52561 ttgttgaaga gtacggagtg actcgttata agaaatttga gcaaagtctt caaattctca
    52621 gaaacttaaa tcgttctatc ctcggtgtag caacctttaa attataagta ttctgatgta
    52681 taaggtcttc aaaatcggct cttagccgat tttgaaggct ttttctatct gtctgcagaa
    52741 attttctctt ggagtctcct atagaaagac cctagggaaa atctatccgt atagaggagc
    52801 cttcttttga taaaatcgta aaaatctagt ataatagata gaactgaaag tatgaggtta
    52861 ctagcaatga aaatgaaaca aattagtgat acaactttaa aaatcacgat gtctttagag
    52921 gatttgatgg atcgtggaat ggagattgct gactttctcg ttcctcaaga aaaaacagaa
    52981 gagttctttt atgctatctt ggatgagcta gagatgcctg atagctttct ggatacaggc
    53041 atgttgagct tccgtgtgac tccaaaacct gataaggtag atgtctttgt gaccaagtca
    53101 aagattgacc aaaatctaga ttttgaagac ttatcggatt tgccagatat ggaagaattg
    53161 gctcaaatgt ctccagatga atttatcaaa accctggaaa aaagcatcgc agacaaaacc
    53221 aaggatgata tcgaagccat tcaatctctt gagcaagttg aagccaagga agaagagcaa
    53281 gagcaggctg aacaagaagc tgagagtaag aaagaacctt acatctacta catcctttct
    53341 tttgctaagt tggctgactt ggtagttttt gccaagacag tgacttttga aatggaaact
    53401 tctgaactct acaaaatgaa cgagcgctat tatttgacca ttttagtgga tattgaaaat
    53461 catccaagcc catatccagc ttggctgttg gcccgtatgc gcgagtttgc agacgatagt
    53521 gatatcagtc gctcagtctt acaagagtat ggtcaagtct tgatgagtca cgatgcagtg
    53581 ctcaatctgc aaaaaatcgg ctaatccttt ctggaaagct attttcagat tttaagaaga
    53641 gcgaatcgtc tgattcgttt tttcttttta tactgaaata gtgatttact ataataggaa
    53701 ttttcacaaa attctgttat aatggctata ttagaaaatt tcgaggagat aaagatgaca
    53761 gttaaaattg ctttactggg atttggtacc gttgcaagtg gtgtgccttt cctcctaaag
    53821 gaaaatggag gaaaaatcaa tcaatcagca cattcagata tcaaagttgc taaggtattg
    53881 gtcaaggatg aagatgaaaa aaatcgcttg cttgcagcag ggaatgactt taactttgta
    53941 accaatgtgg atgatatttt atcagaccag gatattacta tcgtagtgga attgatgggg
    54001 cgtattgagc ctgctaaaac ctttatcact cgtgccttgg aagctggaaa acacgttgtt
    54061 actgctaaca aggacctttt agctgtccat ggcgcagaat tgctagaaat cgctcaagct
    54121 aacaaggtag cactttacta cgaagcagca gttgctggtg ggattccaat tcttcgtact
    54181 ttagcaaatt ccttggcttc tgataaaatt acgcgcgtgc ttggagtagt caacggaact
    54241 tccaacttca tggtgaccaa gatggtggaa gaaggctggt cttacgatga tgctcttgcg
    54301 gaagcacaac gtctaggatt tgcagaaagc gatccgacga atgacgtaga tgggattgat
    54361 gcagcctaca agatggttat tttgagccaa tttgcctttg gcatgaagat cgcctttgat
    54421 gatgtagccc acaagggaat ccgcaatatc acaccagaag acgtagctgt agctcaagag
    54481 tttggttacg tagtgaaatt ggttggttct attgaggaaa cttcttcagg tattgctgca
    54541 gaagtgactc caaccttcct acctaaagcg cacccacttg ctagtgtgaa tggcgtaatg
    54601 aacgctgtct ttgtagaatc tatcggtatt ggtgagtcta tgtactacgg accaggtgcg
    54661 ggtcaaaaac caactgcaac aagtgttgta gctgatattg tccgtatcgt tcgtcgtttg
    54721 aatgatggta ccattggcaa agacttcaac gaatatagcc gtgacttggt cttggcaaat
    54781 cctgaagatg tcaaagcaaa ctactatttc tcaatcttgg ctctagactc aaaaggtcag
    54841 gtcttgaagt tggctgaaat cttcaatgct caagatattt cctttaagca aatccttcaa
    54901 gatggcaaag agggtgacaa ggcgcgtgtc gttatcatca cacacaagat taataaagcc
    54961 cagcttgaaa atgtctcagc tgaattgaag aaggtttcag aattcgacct cttgaatacc
    55021 ttcaaggtgc taggagaata agatgaagat tattgtacct gcaaccagtg ccaatatcgg
    55081 gccaggtttt gactcggtcg gtgtagctgt aaccaagtat cttcaaattg aggtctgcga
    55141 agaacgagat gagtggctga ttgaacacca gattggcaaa tggattccac atgatgagcg
    55201 taatctcttg ctcaaaatcg ctttgcaaat tgtaccagac ttgcaaccaa gacgcttgaa
    55261 aatgaccagt gatgtccctt tggcgcgcgg tttgggttct tccagctcgg ttatcgttgc
    55321 tgggattgaa ctagccaacc aactgggtca actcaactta tcagaccatg aaaaattgca
    55381 gttagcgacc aagattgaag ggcatcctga caatgtggct ccagccattt atggtaatct
    55441 cgttattgca agttctgttg aagggcaagt ctctgctatc gtagcagact ttccagagtg
    55501 tgattttcta gcttacattc caaactatga attacgtact cgcgacagcc gtagtgtctt
    55561 gcctaaaaaa ttgtcttata aggaagctgt tgctgcaagt tctatcgcca atgtagcggt
    55621 tgctgccttg ttggcaggag acatggtgac cgctgggcaa gcaatcgagg gagacctctt
    55681 ccatgagcgt tatcgtcagg acttggtaag agaatttgcg atgattaagc aagtgaccaa
    55741 agaaaatggg gcctatgcaa cctacctttc tggtgctggg ccgacagtta tggttctggc
    55801 ttctcatgac aagatgccaa caattaaggc agaattggaa aagcaacctt tcaaaggaaa
    55861 actgcatgac ttgagagttg atacccaagg tgtccgtgta gaagcaaaat aaagaataga
    55921 agataggatg gggaaactct tgaccagagg gtttcatatc ctttttgtga aaagaagttt
    55981 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggctgct caaaacagtg
    56041 ttttgaggtt gcagatagaa ctgacgaagt cagctcaaga cactgttttg aggttgcaga
    56101 tagaactgac gaagtcagta acatatatac ggtaaggtga cgctgacgtg gtttgaagag
    56161 attttcgaag agtattagtt aaaaacgtga taaaggagaa ataaaaatgg cagaaattta
    56221 tctagcaggt ggttgttttt ggggcctaga ggaatatttt tcacgcattt ctggagtgct
    56281 agaaaccagt gttggctacg ctaatggtca agtcgaaacg accaattacc agttgctcaa
    56341 ggaaacagac catgcagaaa cggtccaagt gatttacgat gagaaggaag tgtcactcag
    56401 agagatttta ctttattatt tccgagttat cgatccttta tctatcaatc aacaagggaa
    56461 tgaccgtggt cgccaatatc gaactgggat ttattatcag gatgaagcag atttgccagc
    56521 tatctacaca gtggtacaag agcaggaacg catgctgggt cgaaagattg cagtagaagt
    56581 ggagcaatta cgccactaca ttctggctga agactaccac caagattatc tcaggaagaa
    56641 tccttcaggt tactgtcata tcgatgtgac cgatgctgat aagccattga ttgatgcagc
    56701 aaactatgaa aagcctagtc aagaggtgtt gaaggccagt ctatctgaag agtcttatcg
    56761 tgtcacacaa gaagctgcta cagaggctcc atttaccaat gcctatgacc aaacctttga
    56821 agaggggatt tatgtagata ttacgacagg tgagccactc ttttttgcca aggataagtt
    56881 tgcttcaggt tgtggttggc caagttttag ccgtccgatt tccaaagagt tgattcatta
    56941 ttacaaggat ctgagccatg gaatggagcg aattgaagtt cgttctcgtt caggcagtgc
    57001 tcacttgggt catgttttca cagatggacc gcgggagtta ggcggcctcc gttactgtat
    57061 caattctgct tctttacgct ttgtggccaa ggatgagatg gaaaaagcag gatatggcta
    57121 tctattgcct tacttaaaca aataaaacag agagtggggc ttcccacttt cttcatttct
    57181 agaatatgaa tagaagggat ttatgaaata cctattatct tacttcaaac cctacatcaa
    57241 ggaatcaatt ttagccccct tgttcaagct gttagaagct gtttttgagc tcttggttcc
    57301 catggtgatt gctgggattg ttgaccaatc tttacctcag ggagatcaag gtcatctctg
    57361 gatgcagatt ggcctgctcc ttatctttgc agtaattggc gttttagtgg ccttgatagc
    57421 tcaattttac tcagcaaagg cagcagtagg ctttgctaag gaattgacaa acgatcttta
    57481 tcgtcatatt ctttccttgc ccaaggacag cagagaccgt ctgacaactt ctagtttggt
    57541 cactcgcttg acttcggata cctaccagat tcagactggt atcaatcaat tcctgcgtct
    57601 ctttttgcga gcgcccatta tcgtttttgg tgccattttt atggcttatc gaatctcagc
    57661 tgagttgact ttctggttct tagtcatggt tgccattttg accattgtaa ttgtagggtt
    57721 atctcgattg gtcaatcctc tctacagtag tctcagaaag aaaacggacc aactggttca
    57781 ggaaacgcgc cagcaattgc aagggatgcg ggttattcgt gcttttggtc aagaaaaacg
    57841 agagttacag atttttcaaa cccttaacca agtttatgct agattacaag aaaagacagg
    57901 tttctggtct agtttattaa cacctctgac ctatctgatt gtcaatggaa ctcttctcgt
    57961 tattatctgg caaggctata tttcaattca aggaggagtg ctcagtcaag gtgctctcat
    58021 tgctcttatc aattacctct tacagatttt ggtggaattg gtcaagctag ccatgttgat
    58081 caattccctc aaccagtcct atatctcagc caagcgaatc gaggaagtct ttgttgaggc
    58141 tccagaggat atccattcag agttagaaca aaagcaagct accagagata aggttttaca
    58201 agtccaagaa ttgaccttta cctatcctga tgcagcccag ccttctctga gagacatttc
    58261 ctttgatatg actcaaggac aaattctagg tatcatcggg ggaactggtt ctggtaaatc
    58321 aagcttgatt caactcatac ttggacttta tccagtaaac aagggaagaa ttgaccttta
    58381 tctagatgga ttgagtcctc ttaatttgga gcaatggaga tcttggattg cctatgtgcc
    58441 acaaaaggtc gaactcttta agggaactat tcgttccaac ttgactttag gtttaaatca
    58501 agaagtgact gatcaagaac tctggcaagc cttggaaatt gctcaagcta aggattttgt
    58561 cagtgataag gaaggactct tggatgctct agttgaagca ggaggtcgaa atttctcagg
    58621 tggacaaaaa caaaggctgt ctatcgcacg agcagtcttg cgccaggctc cgtttctcat
    58681 cctagatgat gcgacctcgg cactggatac cattaccgag tccaagctct tgaaagctat
    58741 ccgcgaagat ctgccaaata tgagcttaat cttgatttct caacggactt cgacactcca
    58801 gatggcggac cagattctcc tcttggaaaa aggtgagttg ctagctgttg gcaagcacga
    58861 tgacttgatg aaatccagcc aagtctatcg tgaaatcaat gcatcccaac atggaaagga
    58921 ggactagaat gaaacgacaa actgtaaacc agacgctcaa acgtttagcc gtagatttag
    58981 caagccatcc tttcttcctt ttcctagcct ttctaggaac tattgcccaa gttggcttat
    59041 caatttacct acctattctg attgggcagg tcattgacca agtcctagtg gctggttcat
    59101 caccagtttt ttggcagatt tttctccaga tgctcttggt ggtaatagga aatactctgg
    59161 tacaatgggc caatcctctc ctctataatc gtctaatctt ctcttatacc agagatttac
    59221 gggagcgaat catccataag ctccatcgtt taccgattgc ctttgtagat aggcaaggta
    59281 gtggagagat ggttagtcgt gtaaccacgg acatcgaaca gttggcagct ggcttgacca
    59341 tgatttttaa ccaatttttc attggtgttt tgatgatttt ggttagtatt ctagccatgc
    59401 tccaaattca tctcctcatg actctcttag tcttgctgtt gacgccactg tccatggtga
    59461 tttcacgctt tattgccaag agatcctatc atctcttcca gaagcaaaca gagacgaggg
    59521 gaattcagac tcagttgatt gaagaatcgc ttagccagca gactataatc cagtccttca
    59581 atgctcaaac agaatttatc caaagattgc gtgaggctca tgacaactac tcaggctatt
    59641 ctcagtcagc catcttttat tcttcaacgg tcaatccttc gactcgcttt gtaaatgcac
    59701 tcatttatgc ccttttagct ggagtaggag cttatcgtat catgatgggt tcaaccttga
    59761 ccgtcggtcg tttagtgact tttttgaact atgttcagca atacaccaag ccctttaatg
    59821 atatttcttc agtgctagct gagttgcaaa gtgctctggc ttgcgtagag cgtatctatg
    59881 gagtcttaga tagccctgaa gtggctgaaa caggtaagga agtcttgacc agtgaccaag
    59941 ttaagggagc tatttccttt aaacatgtct cttttggcta ccatcctgaa aaaattttga
    60001 ttaaggactt gtctatcgat attccagctg gtagtaaggt agccatcgtt ggtccgacag
    60061 gtgctggaaa atcaactctt atcaatctcc ttatgcgttt ttatcccatt agctcgggag
    60121 atatcttgct ggatgggcaa tccatttatg attatacacg agcatcattg agacagcagt
    60181 ttggcatggt gcttcaagaa acctggctca cacaagggac cattcatgat aatattgcct
    60241 ttggcaatct tgaagccagt cgagagcaag taattgctgc tgccaaagca gctaatgcag
    60301 actttttcat tcaacagttg ccacagggat acgataccaa gttggaaaat gctggagaat
    60361 ctctctctgt cggccaagct cagctcttga ccatagcccg agtctttctg gctattccaa
    60421 agattcttat cttagacgag gcaacttctt ccattgatac acggacagaa gtgctggtac
    60481 aggatgcctt tgcaaaactc atgaagggcc gcacaagctt catcattgct caccgtttgt
    60541 caaccattca ggatgcggat ttaattcttg tcttagtaga tggtgatatt gttgaatatg
    60601 gtaaccatca agaactcatg gatagaaagg gtaagtatta ccaaatgcaa aaagctgcgg
    60661 cttttagttc tgaataagcc attctctttt gaaagtttat ggacgaaaaa agttgccttc
    60721 gagtgacttt tttgttacaa tagctagaaa aattgttcac tgtaatactc aatgaaaatc
    60781 aaagagcaaa ctaggaagct agccgtaggt tgctcaaagc acagctttga ggttgtagat
    60841 aagactgacg aagtcagctc aaaacactgt tttgaggttg cagatagaac tgacgaagtc
    60901 agctcaaaac actgttttga ggttgcagat agaactgacg aagtcagtaa ccacacctac
    60961 ggcaaagtga atctgaagtg gtttgaagag agtacaactt gtcttttaaa aaaggagtct
    61021 ataatgaaag tctttcagca tgtaaatatc gtgacttgtg atcaaaattt ccatgtttat
    61081 cttgatggaa tcttagcagt caaggattct caaatcgtct atgtcggtca agataagcca
    61141 gcgtttttag aacaagctga gcagattata gactatcagg gagcttggat tatgcctggt
    61201 ttggtcaatt gtcacaccca ttctgcaatg acaggtctga gagggatccg agatgacagc
    61261 aatctccatg aatggctcaa tgactatatc tggccagcag aatctgagtt tactcccgac
    61321 atgactacca atgcggtcaa agaagccttg acagagatgc tccagtcagg aacaacaacc
    61381 tttaacgata tgtataatcc cagtggtgtg gatatccagc aaatttatca ggtggtgaaa
    61441 acttccaaga tgcgttgtta tttttctccg actctctttt cttcagagac agaaacaact
    61501 gctgagacta taagcagaac tcgatccatc atagacgaaa tcttaaaata taaaaatcca
    61561 aatttcaagg ttatggtagc acctcattct ccgtatagct gcagtagaga cttgctggaa
    61621 gcgagtttgg aaatggcaaa agagctaaat attccgctcc atgtccatgt ggcggagacc
    61681 aaggaggagt caggaattat cctcaaacga tacggcaaac gcccccttgc ttttcttgaa
    61741 gaactgggtt atttagatca tccgtccgta tttgctcacg gggtcgaatt aaacgagaga
    61801 gaaattgaac gcttggcatc ttctcaagtg gctatcgccc acaatcctat cagtaacctc
    61861 aaactggcat caggaattgc tccaattatc cagctccaaa aagcgggagt agcagtcgga
    61921 attgcgactg actcggttgc ttccaataac aatctagata tgtttgagga aggaaggact
    61981 gcagctcttc ttcagaagat gaaaagtggg gatgccagcc agtttccaat cgaaacagct
    62041 ctcaaggtac tgacaatcga aggggctaag gcccttggaa tggaaaatca gataggaagt
    62101 ctggaagtcg gcaagcaagc agattttctg gtcattcaac cacaagggaa aattcatctc
    62161 caacctcagg aaaatatgct gtctcacctg gtttatgcag ttaaatctaa tgatgtagat
    62221 gatgtttata tcgccggaga acaggttgtt aagcaaggtc aagtcctgac agtagaactt
    62281 taaaagaaaa atcacgaaaa attttaaaaa aagtttgcaa aaatcttgca ttcttttttt
    62341 gactatgcta tacttatata cggtttaaaa aaactgccta agacagtagg ggagctcgac
    62401 tcataaatat cctaccgagg acaagacgta tcatgtaaaa agaagcgtat tgtactttcg
    62461 tgtctaggtt tgggcgcgtt tttctttttg aaaaattccc caagcaaaat aattacggag
    62521 gtgaacacac taatgagtga agcaattatt gctaaaaaag cggaactagt tgacgtagta
    62581 gctgaaaaaa tgaaagctgc tgcatctatc gtcgttgtag acgctcgtgg tttgacagtt
    62641 gagcaagata cagttcttcg tcgtgagctt cgtggaagcg aagttgagta taaagttatt
    62701 aaaaactcaa tcttgcgtcg tgcagctgaa aaagctggtc ttgaagatct tgcatctgta
    62761 tttgttggac catctgcagt agcattttct aatgaagatg ttatcgcacc agcgaaaatc
    62821 ttgaatgact tttctaaaaa cgctgaagca cttgaaatta aaggtggtgc aatcgaaggc
    62881 gctgtcgcat ctaaagaaga gattcttgca cttgcaactc ttccaaaccg cgaaggactt
    62941 ctttctatgc tcctttctgt acttcaagcg ccagtgcgca acgttgctct tgcagtcaaa
    63001 gcggttgcag aaagcaaaga agacgcggct taatcttaag ctacacagcg tagcctagct
    63061 acgaaaaaaa ctattataaa atttaaaact tatttggagg aaataacaat ggcattgaac
    63121 attgaaaaca ttattgctga aattaaagaa gcttcaatcc ttgaattgaa cgaccttgta
    63181 aaagctatcg aagaagaatt tggtgtaact gcagctgctc ctgtagctgt tgctgcagct
    63241 gatgcagctg atgctggtgc tgctaaagat tcattcgacg ttgaattgac atctgcaggc
    63301 gacaaaaaag ttggcgttat caaagttgta cgtgaaatca ctggtcttgg tcttaaagaa
    63361 gctaaagaac ttgttgacgg tgcaccagca cttgttaaag aaggcgttgc aactgcagaa
    63421 gctgaagaaa tcaaagctaa attggaagaa gctggagctt cagttactct taaataagag
    63481 gcatatacca attagaattc ggaatactat agtatcagcc ttttagaatc gtgaacacat
    63541 tttagaaact gataaattaa tttagtttcc tgccaaaaac cctaccaaaa attaatttgg
    63601 cagggttttt ctttttaata aaaatatttt ggagaacagt tgaatattgt tctccttttt
    63661 ttattttctg gaggaaaaat gacaaaagaa ttacaatcat cacgctatat tgtcatttca
    63721 tttttagtac gtgaaatggg aattgacatt gttgaagcca tctctcttat ggctgaatta
    63781 gaaaaaagtg gcttggttcg attggaatca agtggagatt taatactcaa agaacttgga
    63841 ggagcgctat gaaacgaatt accgcaaatc aatatcaaac ttcagaacgg tattataaat
    63901 tacctaaaat tctttttgag gatgagaaat atatggatat gaaactagaa gtaaaggtgg
    63961 cttattctat tttaaaagat cgtttagaat tatctctcag tcgtggttgg atagatgaag
    64021 aaggagcggt ttatttagta ttttctaact ctaaactgat gaaactgtta ggttgttcga
    64081 agtcaaaatt actgtctata aaaaaaattc ttaaagaata tgacttaatt gatgaagtcc
    64141 aacagtcttc cagtgagaaa gggaggctag ctaataagat ttatttaggg gagttgtctt
    64201 ctaccccagt agctagttca aacaggccta gtgttaaaaa aaaaataggg caggttgaaa
    64261 atgaaactgc ccccgtctca cattcagccc ctagtgagac tgaagttagt gagactaaat
    64321 atagtgagac tgattcttta tttagtgagg atgaggagga gaggtatact caacctatct
    64381 tgaaaagaaa agtagaaaag gtcacaaaat atgatcaaga ttatatttgg ggattggtac
    64441 aagatcaatt tagacgagaa ggtttttctg aaacagccag tgaaattgct atgactgatt
    64501 ttgagagaat ctatcagtat gctcttgaca atgttcgctt tgttagaaga gcggaagtac
    64561 ttgctgaatt tgtatttaac ggcttgtatt ctgtttggaa taatcgtgtt agaaaaggag
    64621 gtggttaaat gtcgccaatc gagtggatat tagttatgct cattgtcatt tcaagtggtg
    64681 tgacactttg gacattgata gttgatcaga aaggggtaat caagaaatga gatttattga
    64741 tttattttca ggtatcggtg gttttcgact tggaatggaa agtgtaggac acgagtgtat
    64801 tggattttgt gagattgata aatttgctag agaatcttat aagtccattt ttcaaacgga
    64861 aggagaaata gaatttcatg acatacgaga tgtttcagat gacgaattta aaaaacttag
    64921 agggaaagtc gatatcatct gtgggggatt tccttgtcaa gcattttcaa tcgcaggaag
    64981 acgattggga tttgaagata ttagaggaac tttgttcttt gaaattgctc gggcggccaa
    65041 acaaatccaa ccacgttttc tttttcttga aaatgttaaa ggtctactca atcacgataa
    65101 gggacggacg ttcaccacaa tccttaccac acttgatgag ttggggtttg atgttgaatg
    65161 gcaggtgctt aacagtaagg attttggcgt tccccaaaac agagagaggg tgtttattat
    65221 cggacattct agaaagaaag gtatcagact cttatttcct ttcagacgag aaggtcaagc
    65281 aactaactct gagactttaa aaacattagg aaatttgaat ccatcaaaaa gtggaatgag
    65341 cggtaaagtt tattattcag aaggtcttgc gccaacctta gttcgtggga aaggagaagg
    65401 atttaagatt gcgattcctt gtatgacacc agatagatta gacaagagac aaaatggtag
    65461 acgctttaag gaaaaccaag agccaatgtt tactttaaat actcaggatc gtcatggcat
    65521 tgtcgttgtt ggagatttac cgactagctt taaggagacc ggtcgtgtct atggaagtga
    65581 ggggttatct ccaacactga ctacgatgca aggtggagat aaaatcccca aaatactgat
    65641 tccagaacca attcaatttc taaaagtccg ggaagcaacg aaaaaaggat acgctcaagc
    65701 agaaatagga gattcaatca atttggaaag accaagttct cagtatcgtc gtggtagagt
    65761 tggaaaaggt atagctaata cgttaacaac tagtggacaa atgggagtag tagttgctag
    65821 ttatgaagga gaagataaac aagtttatca tgtagccggt atattaatag atggacaatt
    65881 ttaccgtctg aggatacgac gaatcactcc taaagagtgt tttcgcttgc agggctttcc
    65941 tgattgggct tttgaagctg ctagaagagt ctctagtaat agtcagctct ataaacaagc
    66001 tggtaatagt gtaaccgttc ctgtgattgc cgcaatcgca aagaaattaa aagaaataga
    66061 ggaaaaagat gaaagcatta aataaagaat caatactaga ttgtgatgaa ttagaaacag
    66121 aattacatga tgcagaaatc aaacagctgg atgaacaatt atttttgata cccaattatc
    66181 catgtgagtt tgaagtaaca tttttagatg attaccataa aaaacacaac tacccactat
    66241 tttacgagtc ctatcttcaa aacgtgatgg aattccttga aagtcaagac ataaagaatg
    66301 gggttgatgc ctttgtagat gatcatcaaa atcttgtttt tgttttatat ggacaaggct
    66361 atcgagccga gggaaaagag ggaatactta caacccaagt aactgtaaaa gcttatgatg
    66421 aagacaagaa accgattaac ttctcaaatt tattagattc cttaatcgtc tcagaatatc
    66481 aaatggaacc gaatctttgg gaggtctccc atgattgatc tctatctaag taaaaattgt
    66541 catagaaatc aacttctttt ggatttcttt caaaagtatg gcatagaggt cactcctcat
    66601 tctatttctg aaatgacaaa ggataaatta attgagatga tgagctattc ttcagattgt
    66661 ttcgagtttt tatctcccaa tttattacgt tttaagaatc gagataacct aaaattaaca
    66721 gatttcatcg aaatgatatt aaaaaatcct gaactaacca tcagacttcc tcttgcggtt
    66781 tcaaataagc gagtttatcc aaatttgaat ctggaggagg ctagagcttt attgccaaga
    66841 gatacgaaac aattgattta catggcacaa acacattatt tatctaacta aaggagaatc
    66901 aatatggcag aacgattttg ggagaacttg tccattattt tggctgaaag gaatatcagc
    66961 tggattgagt taaccagaaa aatgtttgcg ggagagtttc actatccaag tgaattgaat
    67021 cgtttgtatc aaaagatacg ccactacaag atggaacaac gaatgcctca aagtccatgg
    67081 gtagaaagga ttgtgcaggt attagattta gattatgaag atttatttcg gaggtaacta
    67141 tgaaaagaat aattccagtt tatatattcc aacaagtaaa tgtcttattg gtatccctat
    67201 acttactgaa attgctttgt attagtgagt taaccatact acagattctc tattgtgcgt
    67261 cgctcatttc ttttttatgg atgtatggtc aacgaaaaca agttgtcaag gtcaatatga
    67321 aaactaggat gaaatggctt ggtattggat tcgttagcct actgattata aatctatgtt
    67381 ttagcctaat ccatgctcaa ggaactacga atcaagcaaa cttaattggc cttcaacatc
    67441 aagtcccttg gttttcattt ttattgttat taatcaatgc gagtatggtt gaagaatttc
    67501 tgtatcgaga aattttatgg aacttggtta gaaaattaga tattcgagtt gctttgacaa
    67561 gtattttatt tgtcttagct catcatccag gaaccatttt agcttggtgt ttgtatgttt
    67621 cacttgggat gtttttaggg cttgtgcgct acaaatcgga cttatggggc agtatggggc
    67681 tacatttggt gtggaaccta tcagtctatg tcttattttt tctttaataa tagctggtct
    67741 gtcttaagca tggattaatt ttgaaataaa caaagtataa atttctaatt atctttttat
    67801 attttcttaa atgctcgtaa agccttattc tatgtgcttt cgagtatttt tactgtagga
    67861 agatacttca cgtttctttg catatttcct catgtcttag ctgtcagaag tggtaaataa
    67921 gtagtaaatt catttgtact actaagcaac aagacgctcc tgttgcttct ctttattcaa
    67981 gcgtttcatt tctgccattg cagaatcgaa tgttgcatgt gcgtaatagt tcagcgtcat
    68041 ggctatatta gcatgtccca taatgtactg taatgccttt ggattcattc ctgcatttgc
    68101 atagttggta cagaatgtat gtcgcaaact atgtggagtg atgtgtggca atttatcctc
    68161 gttatactta ttgtatttct taacaagacc tttcatcatg ccgttgtaat cacttgcact
    68221 tttggatagt tctttctatt aagaaagagg aaatcactat atccatcaat ctcaacacgc
    68281 ttatcattct ttcgattcgc taacactcgc ttaaatgctt gataggcttc ttcaaccata
    68341 ggaacttgac gttcgccact tttggtcttt ggtgtttcaa tgtagtaccc aatttcagta
    68401 tctctcaata gctgatggtc tatattgaca agacgattct caaaatctaa atctggaagt
    68461 gtcaaaccac caaactctga aatacgaaga cctgttttta agagtatcag aatttcatca
    68521 taatttttgc tgtaggtttt atcagctttt gcaaaggcta acagtttttc ttcctgttct
    68581 tctgttagta cggttttagg gacagtatca tcatcaagaa ctgctttcag ttgaaagtca
    68641 aatggattct tccgaacaca atcatcttgt atagcaatat agaatgaagc ctttaaagaa
    68701 cgtttgtagt tattgatggt ttgataagca taaccatttt cactcattct aatagcccat
    68761 tctttagcgt ctgatggctt aatactgtca atacttctta cacctaactt gtctttcttc
    68821 aaaatatcca taagatattt gcgtccagtt tcagtgtttt ttctaacctt tggtctttga
    68881 gcgttctgtt ttgcgtaaag ctggcagagt gtcattttct ttcctacaac atcaatacca
    68941 tcatgaatgt ctttctgtaa ctctgcgatt ttctctctaa gtgagataca atcacgcttt
    69001 cctgctggta ctcggtctgt agccacaagt ttccacgagt aaacaaattg cggttctcca
    69061 aatgaatcta tatatttgta taagtatctt ccgtcttttc gttggctctc tccagtcttt
    69121 aagattcgac ctttattgtc acgtcttttt tctgacatgg catttgctcc tttcctttat
    69181 ggaaagagcc ttgatacgac ttaatactat tttatcatat acaagaccct ttggcgacgc
    69241 tagattgcgt ccaatgtatc tataattttt tcaaattgtt ttcgtttaat ctgaatacga
    69301 ttgccattca taatcagcca atttgcattt ttattttcct ctgccaagcg tcgtagcttg
    69361 ttttcgccaa tacgaaaata ttttgacgct tcttcaatgg ttagggtata acgttcccaa
    69421 ataggaatgt cagtctgctt cataaaatcc tcctttccaa atcacttatt tggatttcat
    69481 aaaagttgtt ttaccagcaa tcgaacagct ttagcaaagc tcacgggagt tccacccctg
    69541 catggttctc atgtagccat actcattgcc tgcgacggtt ttatcacgct cggactattg
    69601 actgtatggg agtatcatta tcacgataag aatgtcgttg caggcaatcc tgctaaagat
    69661 tgcttctcgg atcactaaca tgaatcgctc gctatcttta taagataggt catggcggtt
    69721 agttccgttg gctcttttct tatcgaaacg tattcgatta cttttattca gttttcaaag
    69781 aacaatggct cgttagccta tcaaaacaca ttgaaagctc aatatgcttt ggtggaataa
    69841 caaacctccc tgttcgggaa gcgtggaatg gtttagcacg cttccacgaa aggagagagg
    69901 atattactta atttcaaatg acaaaatctt tgtaatcagt ctggtttcca ttcttccacg
    69961 taagacttca tcaacgacca tactttgatt gccatattca tctttcataa gtcgtaggga
    70021 acgcttcgtt atgtaccctc tgtaatgatg tagaatctgg ttaatcgctt cggtatcgcc
    70081 atctgttgcc tttacaatga gaggaaaggg aatcatagga tattgtgttt tcattcttca
    70141 aattcctcca taaacttttt aattaaggct agtccactgg ttctatgccg atagacagta
    70201 gaacggttca atttcaacag gtctgcaatt tctgaatcgc tcatgtccat aaagtaaaac
    70261 agcagtagaa tttcacgttt cttgtctggc aactcacgta atgcttcact caacaaatca
    70321 ttttcaacgc ctactgataa cccattgagt gtaaaaatct gaaagtcagt tgaatagtta
    70381 tctgttgtcg caaactggct aacaagataa tcgccaacat ccgaaaagga cacctcacgc
    70441 tttgcaatcc ttgaaagata aagcatataa ttctttcgct cgtcttccat agcacgttta
    70501 cagatatagt caaactgatt ttctattgtg gtctgaaaag aagatggttt catgtttctc
    70561 accccctttc tgtctaggaa aggaagtgag ccttgctcgt ttatctcctt tcactcttag
    70621 tcccaatgtg aaagggggat ttgttgcatt actgataaat aaactttgta aaaaagttct
    70681 gaatagccaa aaaagcatat aaacagattt atttctctgt ttacatgctt ctgttattct
    70741 atctatatga tttataaaac cacattggtg gacgtactta tctattgcag atagacgact
    70801 ttttttgaca agaacccaat gtaaggaaat ttattgtata tgatgtactt catggcgacg
    70861 ttgacctcca acaaaccgcc atttggaagt aatatacaat attttaacag cgtaaatagc
    70921 actaccatat aacggttttt tttattggcg tttagtagtg ctttttatta aatataaacc
    70981 tataaaccat ataacacgtt tttctatacc tgtttttaat tcagtaggaa caataaaatg
    71041 tatagaggtg gtctactatg cgtaaaaaag aagataaata tgattttaga gcctttggtt
    71101 tagccattaa agaagctcga ttgaaacgag gtttaactcg tgaacaagtg ggagcattga
    71161 ttgaaattga cccacggtac ttaactaata ttgaaaataa agggcaacac cccagcatac
    71221 aagttcttta tgaccttgta tcgttacttc atgtttccgt tgatgaattt ttcttacctg
    71281 ctaataactt ggtaaaaagc acccgacgat tacagataga gaaatacatg gatagcttta
    71341 cagacaaaga actatcctta atggaatctt tagccagcgg tatcaacgaa gcaagaaaca
    71401 tcgaagacta attaaaagaa tccatacata acggaaagag ccgataaaat gagattgtat
    71461 taatctcatt ttatcggctc tgcgtctttg cgtctggctc tgtaatcaca gttactttga
    71521 actgctttat ttcaattaaa ttttcttgtc tgcatttcgg acaatagagg gggaattttt
    71581 ttaattcagt atcttccctt atctttaatc gtgttttatt tccacataca ggacacaata
    71641 tccacttgta gtttataata actatctcct cctttacact ttaattcaaa tctttattaa
    71701 aaa
//
LOCUS       DAVKGU010000002        63092 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKGU010000002 DAVKGU010000000
VERSION     DAVKGU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.002"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..197
                     /locus_tag="VW077_000077"
     CDS             <1..197
                     /locus_tag="VW077_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU9454177.1"
                     /translation="ELLIVGIKILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVANRW
                     DRGLEAYKDKVYTRGIWIRD"
     gene            593..1456
                     /locus_tag="VW077_000078"
     CDS             593..1456
                     /locus_tag="VW077_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9454178.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            1742..1942
                     /locus_tag="VW077_000079"
     CDS             1742..1942
                     /locus_tag="VW077_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9454179.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            2009..2674
                     /locus_tag="VW077_000080"
     CDS             2009..2674
                     /locus_tag="VW077_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9454180.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            2746..3972
                     /locus_tag="VW077_000081"
     CDS             2746..3972
                     /locus_tag="VW077_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9454181.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            4425..5081
                     /locus_tag="VW077_000082"
     CDS             4425..5081
                     /locus_tag="VW077_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9454182.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            5071..5394
                     /locus_tag="VW077_000083"
     CDS             5071..5394
                     /locus_tag="VW077_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9454183.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMILFILVKHKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            5498..6328
                     /locus_tag="VW077_000084"
     CDS             5498..6328
                     /locus_tag="VW077_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9454184.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            6482..7336
                     /locus_tag="VW077_000085"
     CDS             6482..7336
                     /locus_tag="VW077_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9454185.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            7436..8809
                     /locus_tag="VW077_000086"
     CDS             7436..8809
                     /locus_tag="VW077_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9454186.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            8802..9863
                     /locus_tag="VW077_000087"
     CDS             8802..9863
                     /locus_tag="VW077_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9454187.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            9865..10557
                     /locus_tag="VW077_000088"
     CDS             9865..10557
                     /locus_tag="VW077_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454188.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(10587..11156)
                     /locus_tag="VW077_000089"
     CDS             complement(10587..11156)
                     /locus_tag="VW077_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9454189.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            11291..11905
                     /locus_tag="VW077_000090"
     CDS             11291..11905
                     /locus_tag="VW077_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9454190.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLVGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            11907..13553
                     /locus_tag="VW077_000091"
     CDS             11907..13553
                     /locus_tag="VW077_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9454191.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            13565..14851
                     /locus_tag="VW077_000092"
     CDS             13565..14851
                     /locus_tag="VW077_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9454192.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            14903..15436
                     /locus_tag="VW077_000093"
     CDS             14903..15436
                     /locus_tag="VW077_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454193.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            15514..15984
                     /gene="nrdI"
                     /locus_tag="VW077_000094"
     CDS             15514..15984
                     /gene="nrdI"
                     /locus_tag="VW077_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9454194.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(16277..17539)
                     /locus_tag="VW077_000095"
     CDS             complement(16277..17539)
                     /locus_tag="VW077_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9454195.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDAVFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(17849..18307)
                     /locus_tag="VW077_000096"
     CDS             complement(17849..18307)
                     /locus_tag="VW077_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9454196.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(18304..18744)
                     /locus_tag="VW077_000097"
     CDS             complement(18304..18744)
                     /locus_tag="VW077_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9454197.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            19100..21049
                     /gene="mutL"
                     /locus_tag="VW077_000098"
     CDS             19100..21049
                     /gene="mutL"
                     /locus_tag="VW077_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9454198.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(21374..21841)
                     /locus_tag="VW077_000099"
     CDS             complement(21374..21841)
                     /locus_tag="VW077_000099"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9454199.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(21842..23047)
                     /locus_tag="VW077_000100"
     CDS             complement(21842..23047)
                     /locus_tag="VW077_000100"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9454200.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERSGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(23067..23702)
                     /gene="ribE"
                     /locus_tag="VW077_000101"
     CDS             complement(23067..23702)
                     /gene="ribE"
                     /locus_tag="VW077_000101"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9454201.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(23687..24787)
                     /gene="ribD"
                     /locus_tag="VW077_000102"
     CDS             complement(23687..24787)
                     /gene="ribD"
                     /locus_tag="VW077_000102"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9454202.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVQQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(24921..25035)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25192..25785
                     /gene="ruvA"
                     /locus_tag="VW077_000103"
     CDS             25192..25785
                     /gene="ruvA"
                     /locus_tag="VW077_000103"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9454203.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            25795..26358
                     /locus_tag="VW077_000104"
     CDS             25795..26358
                     /locus_tag="VW077_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9454204.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQVLFELLCMETYQA
                     GLSWETVLNKRQAFREVFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKLFATRANAQ
                     VFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            26355..27032
                     /locus_tag="VW077_000105"
     CDS             26355..27032
                     /locus_tag="VW077_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9454205.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(27187..28218)
                     /locus_tag="VW077_000106"
     CDS             complement(27187..28218)
                     /locus_tag="VW077_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9454206.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCPKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(28473..29156)
                     /locus_tag="VW077_000107"
     CDS             complement(28473..29156)
                     /locus_tag="VW077_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9454207.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(29168..30112)
                     /locus_tag="VW077_000108"
     CDS             complement(29168..30112)
                     /locus_tag="VW077_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9454208.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            30244..33075
                     /gene="uvrA"
                     /locus_tag="VW077_000109"
     CDS             30244..33075
                     /gene="uvrA"
                     /locus_tag="VW077_000109"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9454209.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKADVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            33068..34129
                     /locus_tag="VW077_000110"
     CDS             33068..34129
                     /locus_tag="VW077_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9454210.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASSLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            34261..34659
                     /gene="spx"
                     /locus_tag="VW077_000111"
     CDS             34261..34659
                     /gene="spx"
                     /locus_tag="VW077_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9454211.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            34725..35294
                     /locus_tag="VW077_000112"
     CDS             34725..35294
                     /locus_tag="VW077_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9454212.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGTATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKIYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            35381..35647
                     /locus_tag="VW077_000113"
     CDS             35381..35647
                     /locus_tag="VW077_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9454213.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            35651..36070
                     /gene="ruvX"
                     /locus_tag="VW077_000114"
     CDS             35651..36070
                     /gene="ruvX"
                     /locus_tag="VW077_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9454214.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            36086..36391
                     /locus_tag="VW077_000115"
     CDS             36086..36391
                     /locus_tag="VW077_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9454215.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            36646..37896
                     /locus_tag="VW077_000116"
     CDS             36646..37896
                     /locus_tag="VW077_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9454216.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            37893..38438
                     /locus_tag="VW077_000117"
     CDS             37893..38438
                     /locus_tag="VW077_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9454217.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            38584..40116
                     /gene="cls"
                     /locus_tag="VW077_000118"
     CDS             38584..40116
                     /gene="cls"
                     /locus_tag="VW077_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9454218.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            40374..41912
                     /locus_tag="VW077_000119"
     CDS             40374..41912
                     /locus_tag="VW077_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9454219.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSIGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            42032..44239
                     /gene="nrdD"
                     /locus_tag="VW077_000120"
     CDS             42032..44239
                     /gene="nrdD"
                     /locus_tag="VW077_000120"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9454220.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            44251..44391
                     /locus_tag="VW077_000121"
     CDS             44251..44391
                     /locus_tag="VW077_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454221.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKYSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            44435..44941
                     /locus_tag="VW077_000122"
     CDS             44435..44941
                     /locus_tag="VW077_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9454222.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            44934..45524
                     /gene="nrdG"
                     /locus_tag="VW077_000123"
     CDS             44934..45524
                     /gene="nrdG"
                     /locus_tag="VW077_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9454223.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELSDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            45521..46138
                     /locus_tag="VW077_000124"
     CDS             45521..46138
                     /locus_tag="VW077_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9454224.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTLAQELSQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAYELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRFARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            46393..46701
                     /gene="rpsJ"
                     /locus_tag="VW077_000125"
     CDS             46393..46701
                     /gene="rpsJ"
                     /locus_tag="VW077_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9454225.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            46917..47543
                     /gene="rplC"
                     /locus_tag="VW077_000126"
     CDS             46917..47543
                     /gene="rplC"
                     /locus_tag="VW077_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9454226.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            47568..48191
                     /gene="rplD"
                     /locus_tag="VW077_000127"
     CDS             47568..48191
                     /gene="rplD"
                     /locus_tag="VW077_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9454227.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            48191..48487
                     /locus_tag="VW077_000128"
     CDS             48191..48487
                     /locus_tag="VW077_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9454228.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            48505..49338
                     /gene="rplB"
                     /locus_tag="VW077_000129"
     CDS             48505..49338
                     /gene="rplB"
                     /locus_tag="VW077_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9454229.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            49442..49723
                     /gene="rpsS"
                     /locus_tag="VW077_000130"
     CDS             49442..49723
                     /gene="rpsS"
                     /locus_tag="VW077_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9454230.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            49735..50079
                     /gene="rplV"
                     /locus_tag="VW077_000131"
     CDS             49735..50079
                     /gene="rplV"
                     /locus_tag="VW077_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9454231.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            50092..50745
                     /gene="rpsC"
                     /locus_tag="VW077_000132"
     CDS             50092..50745
                     /gene="rpsC"
                     /locus_tag="VW077_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9454232.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            50749..51162
                     /gene="rplP"
                     /locus_tag="VW077_000133"
     CDS             50749..51162
                     /gene="rplP"
                     /locus_tag="VW077_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9454233.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            51172..51378
                     /locus_tag="VW077_000134"
     CDS             51172..51378
                     /locus_tag="VW077_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9454234.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            51403..51663
                     /gene="rpsQ"
                     /locus_tag="VW077_000135"
     CDS             51403..51663
                     /gene="rpsQ"
                     /locus_tag="VW077_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9454235.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            51689..52057
                     /gene="rplN"
                     /locus_tag="VW077_000136"
     CDS             51689..52057
                     /gene="rplN"
                     /locus_tag="VW077_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9454236.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            52135..52440
                     /gene="rplX"
                     /locus_tag="VW077_000137"
     CDS             52135..52440
                     /gene="rplX"
                     /locus_tag="VW077_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9454237.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            52464..53006
                     /gene="rplE"
                     /locus_tag="VW077_000138"
     CDS             52464..53006
                     /gene="rplE"
                     /locus_tag="VW077_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9454238.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            53024..53293
                     /gene="rpsN"
                     /locus_tag="VW077_000139"
     CDS             53024..53293
                     /gene="rpsN"
                     /locus_tag="VW077_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9454239.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            53578..53976
                     /gene="rpsH"
                     /locus_tag="VW077_000140"
     CDS             53578..53976
                     /gene="rpsH"
                     /locus_tag="VW077_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9454240.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGSNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            54168..54704
                     /gene="rplF"
                     /locus_tag="VW077_000141"
     CDS             54168..54704
                     /gene="rplF"
                     /locus_tag="VW077_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9454241.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            54788..55144
                     /gene="rplR"
                     /locus_tag="VW077_000142"
     CDS             54788..55144
                     /gene="rplR"
                     /locus_tag="VW077_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9454242.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            55162..55656
                     /gene="rpsE"
                     /locus_tag="VW077_000143"
     CDS             55162..55656
                     /gene="rpsE"
                     /locus_tag="VW077_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9454243.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            55628..55852
                     /gene="rpmD"
                     /locus_tag="VW077_000144"
     CDS             55628..55852
                     /gene="rpmD"
                     /locus_tag="VW077_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9454244.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            55997..56437
                     /gene="rplO"
                     /locus_tag="VW077_000145"
     CDS             55997..56437
                     /gene="rplO"
                     /locus_tag="VW077_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9454245.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            56450..57760
                     /gene="secY"
                     /locus_tag="VW077_000146"
     CDS             56450..57760
                     /gene="secY"
                     /locus_tag="VW077_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9454246.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            57911..58549
                     /locus_tag="VW077_000147"
     CDS             57911..58549
                     /locus_tag="VW077_000147"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9454247.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            58666..58884
                     /gene="infA"
                     /locus_tag="VW077_000148"
     CDS             58666..58884
                     /gene="infA"
                     /locus_tag="VW077_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9454248.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            58909..59025
                     /gene="rpmJ"
                     /locus_tag="VW077_000149"
     CDS             58909..59025
                     /gene="rpmJ"
                     /locus_tag="VW077_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9454249.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            59043..59408
                     /gene="rpsM"
                     /locus_tag="VW077_000150"
     CDS             59043..59408
                     /gene="rpsM"
                     /locus_tag="VW077_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9454250.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            59426..59809
                     /gene="rpsK"
                     /locus_tag="VW077_000151"
     CDS             59426..59809
                     /gene="rpsK"
                     /locus_tag="VW077_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9454251.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59852..60787
                     /locus_tag="VW077_000152"
     CDS             59852..60787
                     /locus_tag="VW077_000152"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9454252.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            60799..61185
                     /gene="rplQ"
                     /locus_tag="VW077_000153"
     CDS             60799..61185
                     /gene="rplQ"
                     /locus_tag="VW077_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9454253.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            61450..61716
                     /locus_tag="VW077_000154"
     CDS             61450..61716
                     /locus_tag="VW077_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9454254.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            61726..63063
                     /locus_tag="VW077_000155"
     CDS             61726..63063
                     /locus_tag="VW077_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9454255.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
BASE COUNT        18793 a        11896 c        13888 g        18515 t
ORIGIN      
        1 atgaactatt gattgtcggt ataaaaattt tagtatatga accaaattta aacgttagta
       61 tagataacgt aatttttgaa aagaattttg aactatttac aaaacaaagt gatctaattg
      121 ttgcaaatag atgggatagg ggccttgaag cttataaaga taaagtttac acaaggggga
      181 tctggataag agattaacta ttgatattaa aaaggcaata atttataaaa tttgtacaaa
      241 aaaatgttaa attttcacat tttcatagta tactgtttaa tagaaagtag gttctttgta
      301 accgctccat tataaaatcc tttcactctt ccggtatact attaggaaaa acataactgg
      361 aggatatttt gagctcacac ctcatcgttc cagtggctgt tccccaagct cgtgtactgc
      421 ccaaaaaagt tagacattct atttagaacg aggattgagt tctgtattgt acaaggctcg
      481 gtccttttag agtcagctta aggctggctt ttttattttt tctaactatc agaatatttt
      541 gacaaatgaa cacaaataat gatataatat gcaaaagcta ggaggtggta ggatgattga
      601 aaaaatggaa ttgggggaat tttacaagga attacgtttg gcgagaaagc tcaagcagtc
      661 agatgtggct tgtgacgggc taacagcttc tcagttgtcc aagtttgagc tagggcagtc
      721 catgttgtct gctgataagc tcatattagc catccaaggg atcaatgtga cctttgatga
      781 gtttgggcac aagctcaaca actatcaaga atctccacat atgcagattg gtagaaaggt
      841 tgtggatcgc tttgctcatc aagatatagc tggcttagag caactgttgg aggaagtcga
      901 gcaagaacag atggcagaga cttatcgtcg tttgaatgct attgtaatca aagatgccat
      961 ccactcacta gataaaagct acccactagc agaggaggat agcgagtttt tgaccaccta
     1021 tctctatgct atcgagtctt ggacctggtt tgaactctat ctcttttgca atactctgcc
     1081 cttcttgagc aatcaagatc tgatcttttt atcaacctcc ttactcgaaa aatccaaaga
     1141 atttaaagag ttagtacaca atcgattgta tatgaagcaa ggactcttaa atatcatctc
     1201 agagctcatg gagcgcaaac ttttttctta tatcccaatc tttgaagccg agctggagag
     1261 tatgctccgt ccatacgatg tttttgagaa agtgttgtgg cagtttttaa agaagatgag
     1321 cattttcctt caaactaagg gaagcaatca aaaagagatt gaacacttta tccaatctct
     1381 gcaagtatta gaaaatccac aattaatagc cctttttgaa ttgcgtttgc agcaatacaa
     1441 aaagcttatc gattagttag aaatggcaaa ataataataa tctcagcaac gattttcaat
     1501 aatgttaaaa tttgtttctt catatttctc cactcctcat tagtagtaac tttattatac
     1561 cagagaagta atcaaattca ccaaaatcgc aaatctgaaa tttctataag aaaaaatatc
     1621 aaatatgcga ttttctaaaa taagccaatt ttcgtgttat actgtacttg taaagcactt
     1681 gaagcaaatc ctaggtcgca gaagtggttt acaaatgaag ataattgaag gagtgtaaaa
     1741 gatgttaaat ttacaatttg cagaaacaat ggaattgaca gaagctgagt tggagatagt
     1801 ttatggaggg gaatttggga ataatgctgt tatcccagcc ggtgcttggg gagggttcgg
     1861 gacaccttgg tcaatcacta atttctggaa gaaaaatttt aacgatagac ctgactttga
     1921 ttctgaccgc cgtcgttatt aatatccaaa gcccttgcct tttcttgggt aggggctgtc
     1981 tttatttcat agtataggag gaaatagaat gaaaaagata atctcacacc gctactttat
     2041 tatagttttt ctactagtta ttgctgacca gaagttcagt gttctagttt tacgcagcga
     2101 ccttgttact gggctatctg actttgccta ttatctgtcg gatatgatgt tgaattttct
     2161 tgtggtttta tttgctctta ttgctatgat ttggtcgggg aaatggcaga aaatcaatag
     2221 tagaaagttt aaaggatcct atcttttcta ttccttccta gctctgcttg cttttgttgt
     2281 ttggaatttc gttacctttt ttcttttccc acctactcga aatgaaattt cttatcaaca
     2341 tgctgctccg acttttacag gggctacagc atttttgatg tattttttct atcctgtgat
     2401 tgcaggtccc atttttgaag agataatcca tcggggattg gtgatgacag ctctggaaaa
     2461 aggaaagaaa tgggggctag atgtacttgg ctctgctatt ttatttgggg tctcgcacat
     2521 tagtaatcac ggttgggtct tgacagactt tgtcttctat atgggtggag gtcttatatt
     2581 tgcagtctta tttagaatga caaagtcaat ttattggcct attggactgc atatagtcta
     2641 caatggtatt ggtcagcttt tgatgttact gtaattttgg ttgttagtag tatcgcggtc
     2701 tattcttggg gaaagattcc catttgaact taagaaggag ttgtaatgaa agtatttctt
     2761 caaaatagag attttaggca attaactatc aaccagtgga tttcaacgct tggggatacg
     2821 attttttatc tggccttcct gaattatgtg gcagatgcgt cttttgcccc tttggcgatt
     2881 ttacttatca cgatttcaga aacccttccc caagttctac aaatctttct gggagttttg
     2941 gcggattttc aacatcatcg tgtcttgaaa tacacagtca ttagttttac aaaatttttg
     3001 ctttactcta tagtgtccct atcgctttca gggcagtctt tttccttgtt gctagtaacc
     3061 tttatttgta tgattaacct cttgtctgac acattgagtt atttttcagg cgccatgctc
     3121 actccgattt tcattagaat tattgggcaa gatcatctgg cagaagctat tggatttaaa
     3181 cagtcaactg ttagtttagt gaaaacaatc agtaatatcc taggaggagt cttactaggt
     3241 attctatcca tccagtttat ttccttactg aacgctctta cctttttaat cgcattttta
     3301 ggtatcctct tcataaaaac tgacctcttg aaagtagaaa aaacgattag ctatcaagaa
     3361 ggactctctg taaaatcctt ttgccagcat ttgctccaat catcgaaatt gatatggaat
     3421 atgaataagg tgctcttggt tttgtttatt atctctacta gtcaagcagt gataaatgtt
     3481 acagttcctg tctctactct atttttgagg aatcaaccct ttttgaattt acaaacaggt
     3541 caatctcttg ccttgctatc cacttttgaa ctgtcagccc tgattgtcgg aagccttgta
     3601 agtggctatc tgcaacatac catttccata aaaacagctc tctatgcctc acttgtcatc
     3661 cagttgcttc ttctagtagg atttgccaca gttcgttttg actggattct catctttagt
     3721 accttggatg cctttttcgc aggtgttctc tctcctagat tgcaagaact cgtctttaaa
     3781 caaatacctg aggagtcaat gggagcagtt caatcttcta tcagtgccat tacggttgtt
     3841 ttacctagct tatttacaat atctttggta accattgcta ctagctttgg aactctggca
     3901 gttagctttg ttttattgct atttcttcta gttgcctttg ttatgcttct gaatattcgt
     3961 gaaagtattt agtggagaat ttctataata tatgtttgag actggattgc cagtcttttt
     4021 atgatggtca aaatcaacag actttttctc ttttctgaat gtgttatagt agactgaatc
     4081 taaaatagta cgaaacaatt gctaaaacat ttatagaaat taattttact ttcccaatcg
     4141 atttgttctc atcttatttc aatctgctat ataatagtcc atgctgtggc tggattcttt
     4201 tcagcctact tatactcttc gaaactctct tcaaaccacg tcagcttcac cttgccgtat
     4261 atgtgttact gacttcgtca gttctatcca caacctcaaa acggtgtttt gagcaacctg
     4321 cggctagttt cctagtttgc tctttgattt tcattgagta ttattaaaac aatatgagga
     4381 gaaagatgaa agaaaaagga ttttaggagg gggcgcaggc agccatgcca acggctcttg
     4441 gctatgtcag tatcggcctg gcctgtggga ctatcggtgc tccctatgtg acacctgttg
     4501 agatgggctt gatgagtctc tttgtttatg ctgggagtgc ccagtttgcc atgttggcac
     4561 tgattgtggt tcaagctcct gtggcagcta ttgctatgac ggtttttcta atcaacttgc
     4621 gtctcttttt gttgagttta cacgcatcga cttatttccg tcataccagt ctttggtaca
     4681 atatcggtat gtctagtatc ttgacagatg agacctatgg cgttttgatg ggtgaattgg
     4741 cccatacaga caaggtaaat cctatgtgga tgcacgggaa caatctcaac agctatgtgg
     4801 cttggtttgt ggggacagta gtcggaacgg ctctgggtgg cctgctacca aatccagaaa
     4861 tctttggctt ggattttgcc ctggttggga tgtttattgg tatttttgct tcgcaatttc
     4921 agattatgca aagacggatt cctgtccgca atctgctcat tatcctagca gttgttgcgg
     4981 tgtccttctt tttgctcttg acagtggtgt ctcagtcact agctgttctg tttgcgacgc
     5041 tacttggttg tagcatgggg gtggttttag atggtcagta agtatctttt attagcagtt
     5101 attttctctg gtcttgtgac ctggattccc cgtatgattc tcttcatctt ggtcaagcat
     5161 aagggcctgc ctgcgattgt tgagcgtttt ttgaagtttt tgcctgtttc cattatcttt
     5221 gccttgattc tttcaagcgt agtgacaggt aaggttggga gccttcctca aattaaatgg
     5281 ctagacttct tagccgtctt tccaacagct tgggtagcct ttcgctaccg caatctagtc
     5341 ggaacagttc tctttggagt ggtcttgatt gccatcttgc gtttggtctt ttaaattacc
     5401 cataaaaaaa acttatcaca gagatagata tcatataatg gcgtaaatgc tctttttctg
     5461 ttaagattat aaggtattct attttggagg aaatgacatg aaaaaaatcg ttaaatactc
     5521 atctcttgca gcccttgctc ttgttgctgc aggtgtgctt gcggcttgct caggtggtgc
     5581 taagaaagaa ggacaagcag ctagcaagaa agaaatcatc gttgcaacca atggatcacc
     5641 aaggccattt atctatgaag aaaatggcga attgactggt tacgagattg aagtcgttcg
     5701 cgctatcttt aaagattctg acaaatatga tgtcaagttt gaaaagacag aatggtcagg
     5761 tgtctttgct ggtcttgacg ctgaccgtta caatatggct gtcaacaatc ttagctacac
     5821 taaagaacgt gcggagaaat acctctatgc cgcaccaatt gcccaaaatc ctaatgtcct
     5881 tgtcgtgaag aaagatgact ctagtatcaa gtctctcgat gatatcggtg gaaaatcgac
     5941 ggaagtcgtt caagccacta catcagctaa gcagttagaa gcatacaacg ctgaacacac
     6001 ggacaaccca actatcctta actatactaa ggcagatttc caacaaatca tggtacgttt
     6061 gagcgatgga caatttgact ataagatttt tgataaaatc ggtgttgaaa cagtgatcaa
     6121 gaaccaaggt ttggacaact tgaaagttat cgaacttcca agcgaccaac aaccgtacgt
     6181 ttacccactt cttgctcagg gtcaagatga gttgaaatcg tttgtagaca aacgcatcaa
     6241 agaactttat aaagatggaa ctcttgaaaa attgtctaaa caattcttcg gagacactta
     6301 tctaccggca gaagctgata ttaaataatt tcttgaaatc atccattctg agtaagatgg
     6361 gtggtttctc agtttttagg gatatagttt taaactatat atctgactat ctaataacaa
     6421 tttgtgaaat caaacaaatt gtgagatact agtacggtat tatttttaag gagaaagaat
     6481 catgaaaatc aaaaaatggc ttggtctagc agcccttgct acagtcgcag gtttggctct
     6541 tgcagcttgc ggaaactcag aaaagaaagc agacaatgca acaactatca aaatcgcaac
     6601 tgttaaccgt agcggttctg aagaaaaacg ttgggacaaa atccaagaat tggttaaaaa
     6661 agacggaatt accttggaat ttacagagtt cacagactac tcacaaccaa acaaagcaac
     6721 tgctgatggc gaagtagatt tgaacgcttt ccaacactat aacttcttga acaactggaa
     6781 caaagaaaac ggaaaagacc ttgtagcgat tgcagatact tacatctctc caatccgcct
     6841 ttactcaggt ttgaatggaa gtgccaacaa gtacactaaa gtagaagaca tcccagcaaa
     6901 cggagaaatc gctgtaccga atgacgctac aaacgaaagc cgtgcgcttt acttgcttca
     6961 atcagctggc ttgattaaat tggatgtttc tggaactgct cttgcaacag ttgccaacat
     7021 caaagaaaat ccaaagaact tgaaaatcac tgaattggac gctagccaaa cagctcgttc
     7081 attgtcatca gttgacgctg ccgttgtaaa caataccttc gttacagaag caaaattgga
     7141 ctacaagaaa gcacttttca aagaacaagc tgatgaaaac tcaaaacaat ggtacaacat
     7201 cattgttgcg aaaaaagatt gggaaacatc acctaaggct gatgctatca agaaagtaat
     7261 cgcagcttac cacacagatg acgtgaaaaa agttatcgaa gaatcatcag acggtttgga
     7321 tcaaccagtt tggtaataag aaacagggag gtgggagaga gaattccacc tcttgctttt
     7381 gtatagagca cggagtgtaa agaagactat tcgttcatag aaaggtagag agaatatggt
     7441 ttttcctagc gaacaagaac agattgaaaa atttgaaaag gatcatgtag tccagcatta
     7501 ttttgaggtt ttgcgtacct tgatttctaa gaaatcagtc tttgcccagc aggttggact
     7561 caaggaagtc gcaaattatc tgggtgagat tttcaagcgt gttggagctg aagtggagat
     7621 tgatgagagc tatacagcgc cctttgtcat ggcacatttc aagagttcgc gtccagatgc
     7681 caagaccttg attttctata accactatga cactgtgcca gcggatgggg atcaggtctg
     7741 gacagaggat ccttttacgc tttcggtccg caatggcttc atgtatgggc gtggagttga
     7801 tgacgacaag ggtcatatca cagctcgctt gagtgctttg agaaaatata tgcagcacca
     7861 tgatgattta cctgtcaata tcagctttat catggaggga gcggaggaat cggcttcaac
     7921 agacctagat aagtatttgg aaaagcatgc agacaaactc cgtggggcgg atttgttggt
     7981 ctgggaacaa gggaccaaaa atgccttgga acagctggaa atttctggtg gcaataaggg
     8041 gattgtgacc tttgatgcca aggtaaaaag cgctgatgtg gatatccact cgagttatgg
     8101 tggtgttgtg gaatcagctc cttggtatct cctccaagcc ttacagtctc ttcgtgctgc
     8161 ggatggccgt atcttggttg aaggcttgta cgaagaagta caagagccca atgaacgaga
     8221 aatggccttg ctagaaactt atggtcaacg aaacccagag gaagttagtc ggatttatgg
     8281 attggagttg cctctcttac aggaggagcg gatggccttt ctaaaacgtt tctttttcga
     8341 gccagcgctt aatatcgaag gaatccagtc tggttatcaa ggtcagggtg tgaagactat
     8401 tttacctgca gaagccagtg ccaagctaga ggttcgtctg gttccgggcc tagaaccgca
     8461 tgatgttctg gaaaaaattc ggaaacagct agacaaaaat ggctttgata aggtagaatt
     8521 atactatacc ttgggagaga tgagctatcg aagcgatatg agcgcaccag ccattctcaa
     8581 tgtgatcgag ttggccaaga aattctatcc acagggcgtt tcagtcttgc cgacgacagc
     8641 ggggacagga cctatgcata cggtctttga tgccctagag gtaccaatgg ttgcattcgg
     8701 tctaggaaat gccaatagcc gagaccacgg tggagatgaa aatgtgcgaa tcgctgatta
     8761 ttacacccat atcgaattag tagaggagct gattagaagc tatgagtaga gatattatca
     8821 agttagatca gatcgatgtg acttttcacc aaaagaagag aaccatcaca gcggttaagg
     8881 atgtgaccat tcacatccaa gaaggggata tctacggaat cgttggatat tctggagcag
     8941 ggaaatcaac ccttgtacgg gtgattaatc tcttgcaaaa accatctgca gggaaaatta
     9001 ccattgacga cgatgtgatt tttgatggta aggtcacctt gacggcagag cagttgcgtc
     9061 gtaaacgtca agatatcggg atgattttcc agcattttaa cctgatgagt caaaagacag
     9121 cagaggagaa tgtagccttt gcccttaaac actctggact cagcaaggaa gaaaagaagg
     9181 ctaaagtagc taagttgttg gacttggttg gtttggcaga tcgtgctgaa aactaccctt
     9241 cacaattatc tggagggcaa aaacagcgtg tggcaattgc gcgtgccttg gccaatgatc
     9301 caaaaatctt gatttcagac gagtcaactt ctgcccttga ccctaagaca accaagcaga
     9361 ttttggcctt gttgcaagat ttgaaccaaa aattaggctt gactgttgtc ttgattacgc
     9421 atgaaatgca gattgtcaaa gacattgcca accgtgttgc agttatgcag gatgggcatt
     9481 tgattgaaga gggtagtgtg cttgaaatct tctcaaaccc taaacaacct ttgactcaag
     9541 actttatctc aacagctaca ggtattgacg aagccatggt caaaatcgag aagcaagaaa
     9601 tcgtggaaca cttgtctgaa aacagtctct tggtgcaact caagtacgct ggagcttcaa
     9661 cagacgagcc acttttgaat gaattgtaca agcattacca agtaatggct aatattctct
     9721 atgggaatat cgaaattctc gatggtactc ctgttggaga attggtggtg gttttgtcag
     9781 gtgaaaaagc agcgttggca ggtgcccaag aagccattcg tcaagcaggt gtacaactaa
     9841 aagtattgaa gggagtacag taagatggaa tcattgattc aaacctattt accaaatgtc
     9901 tataaaatgg gttgggctgg tcaggcaggc tggggaacgg ctatctactt aactctttat
     9961 atgacagttc tttccttcat tatcggaggc ttcttggggc tagtggcagg tctctttctc
    10021 gtcttgacag cgccaggtgg tgtcttggag aataaagtcg tattctggat tttagacaaa
    10081 attacctcaa tttttcgtgc ggttcccttt atcatcctct tggcaatctt gtcaccactt
    10141 tctcacttga ttgttaaaac aagtatcggg ccaaatgcag cccttgtccc actttctttt
    10201 gcagtctttg ccttctttgc ccgtcaggtg caggttgtct tggctgaatt ggatggcggt
    10261 gtcattgagg cggctcaagc gagcggagcg actttctggg acatcgtggg tgtttaccta
    10321 tcagaaggtc ttccagattt gatccgtgtg acgactgtga ccttgatttc ccttgttggg
    10381 gaaacagcta tggccggtgc ggttggagct ggtggtatcg gtaacgtagc catcgcttat
    10441 ggatttaacc gctacaatca cgatgtgacc atcttggcaa ccatcattat cattttgatt
    10501 atctttgcaa tccaattctt gggagatttc ttgactaaga aattgagcca taaataaaaa
    10561 agagccgcgt ggctcttttt aactgatcag attttctgtg caaatttttt actcaaggct
    10621 tgtccaatca aggcacccac tagggctccg atgacaatac ttgcgataaa tagaaggaca
    10681 gttccagggt ttggagcgac catgatgcgg tcgatatatt cttgggattt tcctcttgcc
    10741 agaagagtag ccatataggc tttgggcgca atccacataa gcaagattgg tcctgttgta
    10801 ctaaaggcga aaataatgaa agaaaggaag ttctttgttt tgtccttgta ttttcctaaa
    10861 tgagctactt catctgctag gaggccacag ataattccag gaaggaagga accagcaccg
    10921 tgtttagttc ccaagaaaaa gagggcaatg acaaggccga tagtggtaat ggctccaaag
    10981 cgcggaactt ttgcgactag gatcatatag acgctaccgc cgacaagggc agtaaaggca
    11041 ggcgcataaa acatgtttcc agtttggtca aagagattgc ccaaaaggac accaatcccc
    11101 atgcagagga agtaaagaac tgcaaaaagc agtgtagtta agatagattt cttcatgaat
    11161 tgtctcctga taattttttc acaattctca ttgtaccacg gtttggtggg attgtaaatg
    11221 gatactagaa gtttttgaaa acgcttgaaa tatgatataa tggtttcata gttattttta
    11281 ggggaatatc atgggtagat ttttagactt tgtctttaat cgtttctttt tagggatgat
    11341 tgcgacagcc ttcttttggc tattaacttt agtaggaggg attatccttg gtctagcgcc
    11401 agctagtgcc accttgatga gcttatatgc agaacatggt tatagctttc gggaatacag
    11461 tttgaaggag gcttggtctc tttacaagca aaattttgtc tcaagcaacc tgattttcta
    11521 tagcttttta ggtgtgggtc tagttttgac ctatggtttg tatctcttgg tgcaattgcc
    11581 tcatcagacc attgttcatt tgattgcgac ccttttgaat gtcctagtag ttgccctgat
    11641 ctttttggct tatacagtat ctttaaaatt acaagtttat tttgccttgt cctatcgaaa
    11701 tagtctcaaa ttatccttga ttggcatctt tatgagtcta gcagctgtgg ccaagattct
    11761 ccttgggact gtgctacttg tagcaattgg ttactatatg cctgccctgc tattttttgt
    11821 aggaattggg atgtggcatt tctttatcag tgatatgttg gaacctgtct atgaaatcat
    11881 ccatgaaaaa ttggcgacaa aatagaatga agcacttttg gctacatacg cttctaagaa
    11941 cctatagttc agtgatgatc attatcattg cgagttttgc aatcttactc tcttacgctg
    12001 actgggattc acgtgaaaag gaagcccaga gagtagccca gcgtgtaact gctagaacag
    12061 tgagtgaaat tgaatattac catagagagt caacccagat agctcaggct ttagttgaaa
    12121 accaagctcg tattgaggga atctataaat actttagcct tagcatgcca gactattttt
    12181 actggcaatt agagcggaaa gcttcgcctt atatatcagt ctctctgtat gaaaatgttg
    12241 atgacctcta tgttcgaaat gattttgtaa ctggggtggc cattgctttt caagattaca
    12301 aggaagtcta tgtttctact aaagacaaac gtagtggaga aaaaatcagg gctgaggatt
    12361 tcaaaccagc aggaaatagt tttgccattc cggtgtcaga tccagtgtca gatcaagatt
    12421 taggagtgat ttacatctcc ttggatcctg ctgttttata ccatgccatt gataatacta
    12481 gaggtcatac tccgatggca gtaacagtga cctcaccttt tgatacggag atttttcata
    12541 ttggtgagac agttgatagg gagagtgaaa attggctagt tggcttaact tctcatggat
    12601 atcaggttca ggtggcagtt cctaaaaact ttgttttaca aggaacagtg actagctctg
    12661 ctttgattgt gggtttgagc cttctcttta ttgtcattct ttatctgact ttgaggcaga
    12721 cttttgctaa ttaccaaaag caggtagtgg atttagtaga atccattcaa gtcattgctc
    12781 aaggcgaaga ggggcgtcgg attgacattt ccgagaaaga tcaggaatta ctcctaatcg
    12841 cggagacgac caatgatatg ttggatcgat tggaaaagaa tatccatgat atttaccagt
    12901 tagagcttag tcaaaaagat gccaatatgc gagccttgca ggcgcaaatc aatcctcatt
    12961 ttatgtataa tacgctggag ttcttgcgca tgtatgcagt tatgcagagt caagatgagt
    13021 tggcagatat catttatgaa ttcagtagtc tcttgcgtaa caatatttcc gacgaaagag
    13081 agaccctcct caaacaggaa ttagaatttt gccgtaaata cagctatctc tgcatggttc
    13141 gctatcccaa gtccattgcc tatggtttca agatagatcc agagttagag aatatgaaga
    13201 ttcccaagtt taccttgcaa ccgctggtag aaaactattt cgcgcatggt gttgaccaca
    13261 ggcggacaga taatgtgatt agcatcaagg ctcttaaaca ggatggtttt gtggaaattt
    13321 tggtggtcga taatggtaga ggaatgtcgg ctgaaaagtt ggcaaatatc cgagaaaaat
    13381 taagtcagag atattttgaa caccaagcca gctacagtga tcaaaggcag tctatcggga
    13441 ttgtcaatgt acacgagcgt tttgtgctct attttggaga ccgctatgcc attactatag
    13501 agtctgcaga gcaagccggt gttcagtatc gtattacaat tcaagatgag tagaaaggga
    13561 gaaaatgtat aaagtattat tagtagatga tgagtacatg gtgacagaag gtctgaagcg
    13621 tttgattccc tttgataagt gggatatgga ggttgtcgca acagccagtc atgccgatga
    13681 agctctagaa tatgttcagg aaaatcctgt cgatgtcatc atttccgatg tcaatatgcc
    13741 agacaaaaca gggcttgata tgattcggga gatgaaagag atcttaccag atgctgccta
    13801 tatcctgctc tcaggttatc aggagtttga ttatgtaaaa agagcaatga accttagtgt
    13861 ggtggactat ttggtcaagc ctgttgataa ggtagagctg ggaaatctgc tggagaagat
    13921 tgcaggtcag ctcggcgaga gagggaagaa aagtcagact cttagtcaag aattagacga
    13981 ggctggattt gttagttatt taggggataa ggagaattgg tggataggtc tatccaagga
    14041 aaaacaaggt tccttcacca ttccctacta tgtcttgggc caagactggc agattttcat
    14101 ttctggccaa cctctagatg gtttagtcgt tacacctttt gaagctcctt atcaagaaca
    14161 ctttgaacgc tggaagctga atgctgagaa aaccctcttt tacggttctg taaatttgca
    14221 gcagtctgag agtctctttg cctattacga accgatttat agggttatca ttcagggaaa
    14281 tctcaatcaa atcgtagaag agttaaatct cttggagaag gtagttcttg aaaatacgcc
    14341 gcgtgttccg attactaaac agctttttat tcagtttgtc atggatgttt tccatttatt
    14401 tgaacatctc aaagctgatg acctgacaga catcgtcaaa accattcatg ctattcaatc
    14461 cttcgatgaa ttggtttctt atatcaagga aactctgatc agctttttcg gtcaataccg
    14521 tatgaatgaa aatgtggtca gtgtgctgga agtcattggt cgtgattacc aaaaagagct
    14581 ttccctcaag gatatcagta aggctctctt tatcaatcct gtctatctag ggcagttgat
    14641 taagcgtgaa accgattcga cctttgcaga gttactaaac aaacaacgta ttaaggctgc
    14701 ccaacaactt ttgctttcaa ctagtgacag catcgaagat atttgttatg ctgttggtta
    14761 cagtaacctt ggatatttct ataaagtttt ccgaaaattg tgcggaaaat cgccaaaagc
    14821 ctaccgaaaa caggtagaaa ctatactata aaatttgtat tcctttacaa aaggtgctat
    14881 aatattatca ggaggttcta tcatgaaaaa gaaaccaatt tatctatggg tcttgctaat
    14941 cttgtctgcc cttatttcag ttccgtctct gtttggaatt gtgagtccct tgcctagtaa
    15001 ggaagccctt cgtgctgctc agaaacaagt tgcaggggtc aatgctcagc aattagaaga
    15061 ccagcttaat tatacctata gagtagcaga agcatctcat tctattttta atgttgcttt
    15121 gattgtgcta tctactattt tagtggtcgt agcgattgtc ttccttgttc gtaaaaattt
    15181 gcaatatgca aactatactt acgttggcta tgttttgtta gctattattg gttcgattta
    15241 tggctatgtt ggtttacaag acgctgtgca gttggttcaa gatgagagca tgcgtttgac
    15301 agtgagtatt ggttcaaaag ctgttagcat tttctatatc gtcatcaatg ttctcttcct
    15361 agcccttgtc ttctataaga tgtggcgaca acagaaagcc ttggcagaag aagaggaaac
    15421 agaagagctt gcctaagtat tgacaaaaaa gaactatcaa gatacaatct tggtagttgt
    15481 tttttgatta tagataaaat catggaggta catatgaaga caatttcttt agtttatatt
    15541 agtttgagtg gcaacactga aagttttgtg acgcgcttga aagactatct cttgtcccag
    15601 tacaaaggga ttgaggttca aaagattcat atcaaggatt tagtcaagga aggcaaaaat
    15661 ttctatgaaa tggaccatcc ctatgtcgct tttttgccga cctatctcga aggtgggaat
    15721 ggcgtggata acggagatgt tgagattttg acgacaccgg tgggagattt tattgcctat
    15781 ggtaacaatg ctagtaagtg ttttggtgtg gttgggtcag gaaatcgtaa ctttaataac
    15841 caatactgcc taacagccaa gcaatacagt caacgctttg gctttcctgt attggctgac
    15901 tttgaaatgc gaggcatgct ggaagatatc aaacatgtcg cagctattat cgcagatttg
    15961 tatgagttgg aaaaggagaa ttaatactca atgaaaatca aagagcaaac taggaagcta
    16021 gtcgcaggtt gctcaaagca cagctttgag gttgcagata gagctgacgt ggtttgaaga
    16081 gattctcgaa tagtataaag ttcttataag aggtggttga agttgttcaa ctgttttttg
    16141 agtataaaca gtctttaaga acgtaaaaaa gcataagttc aggtcttcaa aaccttgttc
    16201 ttatgctttt tatcatagaa atatttgctg aattttctct tgaaatgaaa ccttatcttc
    16261 atctcgcgct tgtcccttac cctgcttttc cgattgcgag tgcgtagata aagaagatgg
    16321 cgaagccaaa gaggaaaatc aatcccgcaa tggcaaggtg tttgagccaa gaaggaagat
    16381 ttttgttttc acgcgttacc aaggcgtaat tcaaaagagc aaagaaaggt gttgtcagga
    16441 aagagccaat catggcaaag cggagcatgg ttgaaacctg accagcgaag aacttgataa
    16501 tgacgatacc gatgatagca gtgatggtca tccagatgtt caaagattta cgattgtcct
    16561 ctttttgact gattagcagt cggagagatt cctgattaac gcgagaatag ccatcgataa
    16621 cagttataac tgttccaaag atacagagga aggcaataaa ggtaatcaag taacgggacc
    16681 attcgccaag aacagaggca tacatgccca cgaattgaga gatgtatttg gctgaagcag
    16741 cttcaaccgc ctgccctgta ggatactgaa tcagtgctcc cagtgccaca aagaagacgg
    16801 ctaggatagc tgttccaata taaccagtgt taaagtcaaa cacagcgtct tctgtgttaa
    16861 agttgacggt ctttctcttt tcagctgacc aaagtgaatt gatggctgaa atttcaatag
    16921 gagccggcat ccatcctagg agggagacga tgaagggcag agctgccatt tgccaaggtg
    16981 tcttctcgac aaaatcagaa ctgtattctg gatgcttgac cgccgcaatg ataactgcaa
    17041 gaacagtcgc aatggttaag gcagacataa tccatttgac catgccgtct aaaagtttgt
    17101 agcctccaaa gagtagcata gcccaaatga ttgcaacgag aatgagggac cactgagtaa
    17161 tgctaagtcc aatcattggg aaggcactgg cgatgatagc tgagcacaga atggcaacac
    17221 cagccgtgtt gaccatagcc gaaaagacat tgaggataaa gaaaatccag agatagagtt
    17281 ttcctttttc ggcataacct tcaaccaaag tctttccagt atcagctgtg tattcagcac
    17341 caaaacggaa aaatggatat ttaaagacat tggctaagat gaccaagaga agtagagacc
    17401 aaccgtaaga accgccagct tgagttgagg atacaatgtg ggaacctcca acagcggcag
    17461 ttgccattag gattccaggt cccattgctt ttagcttgct tgcccaggtt gattgattta
    17521 aggaaatagc ttgcgacatg ataaatactc ctttttgaat tttcagaata atctgtatat
    17581 gtaatctata ttctacaaaa aattttggaa aaatgcaaga gtattttgaa aaaagataga
    17641 aaatttacaa agaaaatctg aaataactgt tatgatatag cagattgaaa ctagaatagt
    17701 acacttttgc ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatt gatttaccct
    17761 gttcttattt catttcacta tagtaaggtg cagcaggata aagcaaaacc gagaagtttc
    17821 tttctcggtt ttgcttatta tgaggttcct attttctatg ctccaagagt tgattgatgt
    17881 gatctacttt tttagcctct cttttataga gaatagccca gatgatgaga tagacaatcg
    17941 caaactcgat aatgagctgg aggtagaaaa tccagtggaa ggggaaccaa ccagcaagag
    18001 ttgctagtgg gacaaagcca gccagcataa ggaagaaatg agtcagagtg gcacgaagca
    18061 tgctccagtc acggctgaat aagcggttgc caaagttgaa gagcataccg atggctgccc
    18121 agataagtgt gcagtagagc aagaccaggg caccgtgaac ctgatgctga tccatcactt
    18181 ggccgatgag agagtaggga tttagtggtg cgtaggtatt tggtgcataa atgagtgaaa
    18241 agaggatgga gaggatgagg ccgataagaa caccggcagc tgcatcgtga aatacttgtt
    18301 ttttcatagt tctaatttct ccttgatggt ttttagataa cggcgtgaag agtaggtgaa
    18361 gctttcgttt ttcaagaaaa tttctaccag accgtttggc gtgagcttga ggtgagagat
    18421 ggaatcgata ttgatgattt ctgattggga aatttggata aaattggttg gcaagagttt
    18481 aagaacctga tagagtcgca aatcaatgct gtaggtctga ctcgcggttt ctgctagaac
    18541 cttccgattc tcgatataga agcgctgaat cttgccaatc tcaactagat agacctgatc
    18601 atcgattttt cctttgattt tttctctttg gtccagattt tctgcgaact cgatgacttt
    18661 ctggactttt tcggtttctt gaggtgcttg gacaatcagc ttttcctcct cgtaagtctc
    18721 actaatctgt agttctactt tcataaattt ctctccttgt ttttcataca aaattaatct
    18781 cacttcttgt acacctttat taatactctt caaaaatctc ttcaaaccac gtcagcgtcg
    18841 tcttgccgta ggatagttgt tgactttgtc agttttatct acaacctcaa aacagtgttt
    18901 tgagctgtct gcgtctagct ttctagtttg ctctttgatt tttattgagt ataccactat
    18961 tttactccct ctggcaaggg actttgtcta tgtggaggga ttgggctcct atgtggtgga
    19021 gtttttctgt tctttctgaa atatggtata atagcactaa ttaatttcta ggaaaataga
    19081 tacagaaagg ggctgaaaga tgtctcatat tattgaattg ccagagatgc tggcaaacca
    19141 aatcgcagct ggggaggtca ttgaacgtcc tgccagtgtg gttaaagagt tggtagaaaa
    19201 tgccattgac gcgggctcta gtcagattat cattgagatt gaggaagctg gtctcaagaa
    19261 ggttcaaatc acggataacg gtcatggaat tgcccacgat gaggtggagt tggccctgcg
    19321 tcgccatgcg accagtaaga taaaaaatca agcagatctc tttcggattc ggacgcttgg
    19381 ttttcgtggt gaagccttgc cttctattgc gtctgttagt gtcttgactc tgttaacggc
    19441 ggtggatggt gctagtcatg gaaccaagtt agtcgcgcgt gggggtgaag ttgaggaagt
    19501 catcccagcg actagtcctg tgggaaccaa ggtttgtgtg gaggatctct ttttcaacac
    19561 gcctgcccgt ctcaagtata tgaagagcca gcaagcggag ttgtctcata tcattgatat
    19621 tgtcaaccgt ctgggcttgg cccatcctga gatttctttt agcttgatta gtgatggcaa
    19681 ggaaatgacg cggacagcag ggactggtca attgcgccaa gcaatcgcag ggatttacgg
    19741 tttggtcagt gccaagaaga tgattgaaat tgagaactct gacctagatt tcgaaatttc
    19801 aggttttgtg tctttacctg agttgactcg ggctaaccgc aattatatca gcctcttcat
    19861 caatggccgt tatatcaaga acttcctgct caatcgtgct attttggatg gtttcggaag
    19921 caagcttatg gttggacgtt ttccactggc tgtcattcac atccatatcg acccttatct
    19981 agcggatgtc aatgtgcatc caactaagca agaggtgcgg atttccaagg aaaaagaact
    20041 gatgaccctg gtttcagaag ctattgcaaa tagtctcaag gaacaaacct tgattccaga
    20101 tgccttggaa aatcttgcca aatcgaccgt gcgcaatcgt gagaaggtgg agcaaactat
    20161 tctcccactc aaagaaaata cgctctacta tgagaaaact gagccgtcaa gacctagtca
    20221 aactgaagta gctgattatc aggtagaatt gactgatgaa gggcaggatt tgaccctgtt
    20281 tgccaaggaa accttggacc gattgaccaa gccagcaaaa ctgcattttg cagagagaaa
    20341 gcctgctaac tacgaccagc tagaccatcc agagttagat cttgctagca tcgataaggc
    20401 ttatgacaaa ctggagcgag aagaagcatc cagcttccca gagttggagt ttttcggaca
    20461 aatgcacggg acttatctct ttgcccaagg gcgagatgga ctttacatca tagatcagca
    20521 cgctgctcag gaacgggtca agtacgagga gtaccgtgaa agcattggca atgttgacca
    20581 gagccagcag caactcctag tgccctatat ctttgaattt cctgcggatg atgccctgcg
    20641 tctcaaggaa agaatgcctc tcttagagga agtgggcgtc tttctagcag agtacggaga
    20701 aaatcaattt attctacgtg aacatcctat ttggatggca gaagaagaga ttgaatcagg
    20761 catctatgag atgtgcgaca tgctcctttt gaccaaggaa gtttctatca agaaataccg
    20821 agcagagctg gctatcatga tgtcttgcaa gcgatctatc aaggccaatc atcgtattga
    20881 tgatcattca gctagacaac tcctctatca gctttctcaa tgtgacaatc cctataactg
    20941 tcctcacgga cgtcctgttt tggtgcattt taccaagtcg gatatggaaa agatgttccg
    21001 acgtattcag gaaaatcaca ccagtctccg tgagttgggg aaatattaaa agtataaaaa
    21061 agtctgggaa aaattttcaa aatcagaaaa acgcataaaa tcaggtgttc aaaaaccttg
    21121 attttatgcg ttttatcata gaaatattta cttcatgttt tcctaattct tttcgaaact
    21181 ctttttaaac cacgtcagtt ttatcagtaa tctcaaaaca gtgttttgag ctaattttgc
    21241 cagttttgtc tgtaacatcg aagttgtgtt tgaccactct gcgactggtt tcctagtttg
    21301 ctctatgatt ttcacagagt attaaattgc gattttgcca agtttcttta ttcgtctaaa
    21361 agtagagtct gttctatgcg tctaatgtac gaattaggtt gaccatttca atagctcctt
    21421 gtgcacactc agaaccctta tttcctgctt tagtgccagc tcgttctatg gcttgttcaa
    21481 ttgtatctgt cgttagcaca ccaaacataa caggaatttc gctatttaaa ctgatttggg
    21541 cgattccctt agatacctcg ctacatacat aatcataatg acttgtattc cctctaatga
    21601 cagctcccaa gcagataatt gcatcatatt ttttactttt tgccattttt gatgcaatca
    21661 gtggtatttc aaaagctcct ggaacccagg ctacctctat atctttctcg tttacattct
    21721 ctcttttgag attatctagt gctccagata ataattttga agttataaat tcattaaatc
    21781 tcgctacaac aatacctatt ttaatattgt ttgctactaa attaccttca taagtgttca
    21841 tttatttttc ctccatattt aaaatgtgac ccattcgatt tttctttgtt tctaaataaa
    21901 aactatcgta aggattggct tctatttcga ttgatattct actggaaatg gtaattccat
    21961 atttttctaa ctgttcaacc ttgtcaggat tatttgtcag taaatgaagt gactgaagtc
    22021 ccagatcttt aagcatttgt gctccaatat gatattctct taaatcacct tcaaagccta
    22081 atgcaagatt ggcatcaagc gtatccatgc cttgatcttg taaatgatag gcttttaatt
    22141 tattgataag tccaattcct cgtccctcct gtcgcaagta aagtaagaca cccgaaccat
    22201 tctcaacaat cattttcata gctttatcga attgctgtcc acaatcgcaa cgtaaggagc
    22261 ctaaaacatc ccctgttaaa cattcggagt ggacccgaca taatacattg gcttcatcct
    22321 ctatgtttcc cataataaga gcaagatgat gttccccatt tagtttatct atatagctaa
    22381 ttgctttgaa attaccgtat ctagtaggca tattgacagt tgaaactcgt tctaccagct
    22441 gatcatatac ttttctatat tcttgtaatt ctttgatggt aattagtgga atgttgtgtt
    22501 tcttcgagaa ctgaattaaa tcatctgttc tcatcatttt gccatcatga ttcattattt
    22561 cacaacatag gccacactct tttagtccag ctaattttaa taaatcaaca gttgcttctg
    22621 tgtgtccact tctttctaga acaccacctt tttttgcaat taaaggaaac atgtgtcctg
    22681 gcctgcgaaa atcagagggt gttatatctt cagctacaca catacgtgcg gtcagtcctc
    22741 tttcctcggc agaaatacct gtggtcgttt ctttataatc aattgaaact gtaaaagcag
    22801 tcttatgatt atctgtattg ttttcaacca taggtgaaag cattaattga ttagctaaac
    22861 tttcgctcat aggcatacaa attaatcctt tggcataagt agccataaaa ttaacatttt
    22921 ctgttgtagc tgcttgtgca gaacaaatta agtctccttc attttctcta tccttgtcgt
    22981 ctataacaag aacaagtcgt cccttctgca atgcttctaa tgcttcttgt atttttcgat
    23041 attccattga ctgattatcc tttctgctaa aatccatttt gatataatag ttccttagat
    23101 atttctgatt ttggagagtt atccatcagt ttttgcacat atttacctaa gatatcattt
    23161 tcaagattta ctgtactccc gacttgttta ctcttaagaa tggtttgttc caaggtatga
    23221 gggataacag atactgaaaa gtttactttg gagactttag cgacagtcag actaatgccg
    23281 tcaattgtaa tagatccttt ttcaactatt aaatctaaaa tttctttttg tgtgttgatt
    23341 tgataccata cagcattatc atcttttttt attgacgaga tttttcctgt accatcaatg
    23401 tgtcctgtaa cgacgtgacc cccaagtcga ccgttgacag ataaggctct ttctagattc
    23461 acctcacttc catgttttaa tagagtaaga gctgttcgac tccatgtttc attcattaca
    23521 tcaactgtaa aggattgatg attgaaatga gtaactgtaa gacagatacc atttactgct
    23581 atactatcgc ctaaatggat atccgttaat atttttgagg ctttaattga tagtttacaa
    23641 ttacgagagt ctttctgtat tctttcaact tttccgattt cttcaattat tcctgtgaac
    23701 atggataaat cacttcactt tctatgagat agtcatttcc tatttgagaa aatgcataag
    23761 gtttcaatct aatagcgtca tttggcaaag aaataccttc acctccgaca ggaaacttgg
    23821 cactacctcc aaaaattttt ggtgcaatat atattttcag ctcatcaaca atttgttgtt
    23881 ccaaagcact ccaattcatt agactgcccc cttctagaac taggctatca atctgcatgt
    23941 ttcctagatg ttgcattaaa ctcgataagt ctatatgatt gccttttttc tttatggaaa
    24001 gtatttcaca gccatgattt tgatatagct tcattttatt tttgtcttca gaggaagtgg
    24061 caatgtaagt tttaatatca tttgctgttt ttacgatttt agaggtaaga ggagttcgta
    24121 aatgtgtatc gcatatgata cggataggat ttttcccttc ctccaatcta catgtcagca
    24181 aaggatcgtc ttgaataaca gtattgactc ccaccataat tgcactaaca tggtgtcgta
    24241 actgctgcac atgctttctt gcttcttctt cagtaatcca tttggattga tttgttttag
    24301 tggctatttt tccatccatt gacattgcat atttcataaa aacatagggt acatgctggg
    24361 taatatactt tctaaaactt tttattaagt taagacactc attttctaaa attccaacag
    24421 taacttgaag attattttcc tcaagtatct ttactccttt tccagataca ataggattac
    24481 agtctaggct tccaatgact actcttgtaa taccactatc gattatagca tctatacagg
    24541 gaggtgtttt cccgaagtga caacagggtt caagtgttac ataaagcgtc gctccgacag
    24601 gggattctct acagttttta agagcatttc tctcagcatg tgggccacca aaaaactcat
    24661 gataaccttg tccgataatg tgattatctt ttacaataac tgcgccgacc ataggattgg
    24721 gattgacgta accagcccct ttttgtgcca gttttattgc taatttcata tattttgaat
    24781 cgctcatctc actacctcca aaaaaatatg ccttgaatag gggactactc aaggcataca
    24841 aaagaaaact tatgcgatta acaaaaatgc tctgaaatga caagtaatca tttcagagca
    24901 cgcaaaaagc acaaatatac ttttatcttc tttcatccag actatactgt cggctttgga
    24961 atttcaccaa atcatgcctt tcggctcgtg ggctatacca ccggtaggga attgcaccct
    25021 gccctgaaga tagttattca attacagatg attatagtac ttaattttga atatgtcaac
    25081 agataaatac cgattgtttt tgatatactg tatttgtgat aatcgattct cgctcctcgg
    25141 ataaagaaaa tatgatatac tagataaacg aaataagaga gaaggaatac tatgtacgca
    25201 tatttaaaag gaatcattac caaaattact gccaaataca ttgttcttga aaccaatggt
    25261 attggttata tcctgcatgt ggccaatcct tatgcctatt caggtcaggt taatcaggag
    25321 gctcagattt atgtgcatca ggttgtgcgt gaggacgccc atttgcttta tggatttcgc
    25381 tcagaggatg agaaaaagct ctttcttagt ctgatttcgg tctctgggat tggtcctgta
    25441 tcagctcttg ctattatcgc tgctgatgac aatgctggct tggttcaagc cattgaaacc
    25501 aagaacatca cctacttgac caagttccct aaaattggca agaaaacagc ccagcagatg
    25561 gtgctggact tggaaggcaa ggtagtagtt gcaggagatg accttcctgc caaggtcgca
    25621 gtgcaagcaa gtgctgaaaa ccaagaattg gaagaagcta tggaagccat gttggctctg
    25681 ggctacaagg caacagagct caagaaaatc aagaaattct ttgaaggaac gacagataca
    25741 gctgagaact atatcaagtc ggcccttaaa atgttggtca aataggagca gagaatgaca
    25801 aaacgttgtt cgtgggtcaa gatgaccaac ccgctctaca tcgcctatca tgatgaggag
    25861 tggggccagc ccctccatga tgaccaagta ttgtttgagt tgttgtgtat ggaaacctat
    25921 caggcaggcc tgtcttggga aacggtactc aacaaacgcc aagctttccg agaagtcttt
    25981 catagctatc aaattcactc agtcgcagag atgactgaca ctgaattgga agccatgctg
    26041 gagaatccag ctatcattcg aaatagagcc aagctttttg ctacacgcgc taacgcccaa
    26101 gtctttctac agttacaggc agagtacggc tcttttgatg cctatctttg gtcttttgtt
    26161 gaggggaaaa ctgtcgttaa cgatgttcct gattatcgcc aagcgccagc taaaacaccc
    26221 ttatctgaga aattagccaa agatctcaaa aaacgaggct tcaagttcac aggcccagtc
    26281 gccgtattgt cttttctaca ggctgcaggg ctagttgatg accacgagaa tgattgtgag
    26341 tggaaaggtc ttaaatgatg tctaacaaaa ataagggaat tctgattttt gcgattctct
    26401 atacagtcct ctttgtgttt gatggcgtta aattgctggc ttctttaatg ccatctgtca
    26461 ttgcaaatta tcttgtttat gtagttttag ctttatatgg ctccttcttg ttcaaggata
    26521 gattgatcca acaatggaag gagattagaa agactaaaag aaaattcttc tttggcgtct
    26581 taacaggatg gctctttctc attctgatga ctgttgtctt tgaatttgta tcagagatgt
    26641 tgaagcagtt tgtgagacta gatggacaag gtctaaatca gtctaatatt caaagtacct
    26701 ttcaagaaca accactactg atagctgttt ttgcttgtgt cattggacct ctggtagaag
    26761 aattattttt ccgtcaggtc ttattgcatt acttgcagga acggttgcca ggtttactaa
    26821 gcattattct ggtaggactt gtttttgctt tgactcatat gcacagtttg gctctatcag
    26881 agtggattgg tgcagttggt tacttaggtg gaggccttgc cttttctatt atttatgtga
    26941 aagaaaaaga gaatatctac tatcccctac ttgttcacat gttaagcaac agcctctcct
    27001 taatcatttt agctatcagt atagtaaaat gaaataagaa caggacaaat caatcaggac
    27061 agtcaaatcg atttctaaca atgttttaga agtagaggtg tactattcta gtttcaatac
    27121 actgtaacat gtgatgaaaa tgccagtaat gataccgaga aaaaagctga gaaacttttc
    27181 ccagctttat ttgttatagt caaagcgaat gacttgttcc tgtgcatcta catgagcatg
    27241 gaccccaaag ggtacaattg ctcttggagt tgcgtggccg acattcagat tatagacaat
    27301 cgggatattg ctgtcaatga tatctaatag tgcctcttta tagtcgtcat ggaaagtttc
    27361 atccataggt tttccgacca agagtccatt gatgaccgcg aatatgccag tgtcctttaa
    27421 agttagcaac atctttttga agtcttctgg cttaggcttt tcttcgcttg tttcgagcaa
    27481 gaggattttc ccttcccagt ctgacaagtc agggaaaagt ttgtattttg ggcagagttc
    27541 cgtgctatct gcgtatcgag agttgtcaaa gatatcgtag agggattcga ggcaaccacc
    27601 gaggattttc ccctcgaact gagcacttcc ttgcaacaag tcaaaacctg tatttgtatg
    27661 actgacacga ggtgttccca gggccgtggg actaaaatca gttcgttcct cataccaaac
    27721 gtcactaggg cggatttctg aaattcttcc cgtctcaatc aattctttaa agtagtgaag
    27781 gctataggct agcatttctt tgtctaattc acaaatgtct gctaaaaagg attgaccata
    27841 aaaagtcttg attcctaatt tatgcaacat gaggtggttc atggttgtat ccgagaagcc
    27901 aagaaaaatt ttttgcttga taaccttttg gagttggtca ttttcaaaaa gataaggtag
    27961 caagcgatag gtatcgtctc caccgatggc acataggatc atgtcgatgc tatcatcaga
    28021 aaaggcatga atcaaatcct ctgcacgagc ttcaggatgg tccttgataa agtctaatcc
    28081 ttttaacgaa tggggcaaaa agatgggatt gagtcccaga tccttgagac gttggatacc
    28141 caagtccact tcgtgtttga caaaatcctc tccgataatg ccactagata aactaacaat
    28201 accaatagta gaaaccatat ctcatcctcc tagaaataga ttgagcctat tttatcacaa
    28261 aatataagag aaagctatgt gggatttcag aagaatactt ttaatactct tcgaaaatct
    28321 cttcaaacca cgtcagtttt atctgcaacc tcaaagctgt gctttgagca acctgcagct
    28381 agcttcctag tttgctcttt gattttcatt gaatataact caaaaaagta agtgaaaaag
    28441 caaccgcacc tgcagttgct tttatcgttt tcttattctt gatagcgtgt tggtcctgca
    28501 cttgcactac ggatattcaa gcgtttcttg agatagaagc gaagggctag gagggctgct
    28561 ccaataatag ctagtggcaa tggatccagt actgggttaa ggctagctgg taggaagctt
    28621 gttgcaaaga agacaagcaa ccaaaggaac atagaagcta ggataactag tacagatttc
    28681 cagaaaggtg gacgttgact gcgatccata tctggtccat agtattggta aacaaagtag
    28741 tacatcaagt agaaggcaaa tccaccaacc agtccaacta atagaagagt aatcaatcca
    28801 tagccgaaag cttgatctgc cgcaaagaag gttgtgaggg cgctgacaag ggcaaagagg
    28861 ctagtgatga aaagagctga gtccataatc atcagttttg gatcatcatt ttcttttgga
    28921 tgctcttttt cgtactgctc tttgacagtg aagctatgag cccaatgagt tggtgcgccg
    28981 tatagggaac gggcagttgt acctttagat tgctcctcaa ggatttgggg aatgacttcc
    29041 tcaaaaatag tctggatttc agcgtctgtt ttcccatctt tgatgaattg ttgggtagca
    29101 atgtggacaa actcttggtt tttcttagtt aatttttgta gatcaatttg agacatagga
    29161 actcctctta gaaccatttt ttatggatga gatagagagt gagcgagaca ctcatagcaa
    29221 aggcgataaa gacgattaac cagaaggcat ttggctctcc gtttaggggg atttcattat
    29281 ccttaaagtt catcccgtag gcagaaaaga ccatggttgg gatggacatg acgatggtca
    29341 caagggccaa ggttttcatg atgttgttct ggttgttaga aatgatagag gcaaaggtct
    29401 ctgtcataga atgcaagacg tttccataaa tatctgccat ctcgatggcc tgttgggttt
    29461 caatcagggt gtcttcaagc aggtcttcgt cctcaaggta tttcttgata ttgctggttg
    29521 aactggtcaa tttcttaatc acgcgctcat ttgttttgag ggaggccttg aaatagacga
    29581 tagttttttc caattccatg agctcaatca attcttcatt acgagttgat tgatgcagtt
    29641 gactttcgat ttgttcactc ttgcggtcga ttgaacgaag ggctgttagg taaagctctg
    29701 cattgcgata aagaatttga aagataaaac gtgaacgcat gaaggtatag aaattacgca
    29761 atcgacggtt gataaagaca tcaaggacag gtagtggttc caaacacgta gtgataatgg
    29821 tttcctcagt gatgataata ccaagcggga tggttacgta gtaggtgcgg ttatttcttt
    29881 cctccgtgac cggcacgtct acgataatca gggtatactc gtcttcaatg gtaatacgag
    29941 acatttcttc cgcatcgagc ggtgctcgaa ggtcggcaat atcaatatcg aaggtgttgg
    30001 cgatttcgag tgattcattt tgagtcggat tgacgagatt gatccaagta cccggttcaa
    30061 gcgtatcgat ctctttaaat tcagttgttg tagagagaaa aacttgtttc atatccctta
    30121 tcctttctca tttttcagat tttttcacac tgtactatta tactacaaaa tcggcctttt
    30181 gggtaataga atctcacttt ttttggtata atggtaagca ataatggact agaaagaaca
    30241 aagatgcaag ataaaattgt cattcatggg gcgcgtgccc ataatttaaa aaatattgat
    30301 gtggagattc cgcgagacaa gctggttgtc gtgacaggtt tgtcaggttc agggaaatcc
    30361 agtctggcct ttgataccct ctatgcggag ggacaacgtc gctatgtaga gagtttgtca
    30421 gcctatgctc gtcaattctt aggaaatatg gagaaggcgg atgtggatgc cattgatggt
    30481 ctcagtccag ctatttctat cgaccagaaa acgactagca aaaaccctcg ctcgacggtg
    30541 ggaacaacga ctgaaatcaa tgactatctg cgtctcctct acgcacgtgt ggggacgcct
    30601 tactgtatca acggacatgg agctatcaat gcttcttctg tggagcaaat cgttgataag
    30661 gttttggagt tacctgaacg ccagcgcttg cagatcttgg cccctgtcat ccgcaagaaa
    30721 aaaggccaac ataagagtgt tatcgagaag gttcagaaag acgggtatgt tcgtgtccgt
    30781 gtggatgggg aagtctatga tgtgaccgaa gtgccagagt tgtctaagag caagcaacac
    30841 aatatcgatg tcgtggttga ccgtattgtt atcaaggagg gcattcgtag tcgtctcttt
    30901 gattccattg aggctgccct tcgtattgca gaaggttatg tcattatcga caccatggac
    30961 gactctgagt tgttgttctc tgagcattat gcctgtccag tttgtggttt tactgtccca
    31021 gagttagagc ctcgtctctt ctctttcaat gctccttttg gctcttgtag tgagtgtgac
    31081 ggcttgggca tcaagctgga ggtggatact gatttggtag tgccagatgc cagcaaaacc
    31141 ttacgtgagg gagcgttagc accttggaat cctatctcat ccaactacta tccaaacatg
    31201 ttagagcagg ccatgaaagt ctttggagtg gctatggata agccttttga ggacctgtca
    31261 gaagaagata agaacttgat tctctatggg tcagatggta aggaattcca tttccactat
    31321 gagaatgaat ttggtggcgt gcgcgatatc gacattccgt ttgagggagt tatcaataat
    31381 atcaaacgtc gttaccatga aaccaatagc gattacactc gcactcagat gcgtctctac
    31441 atgaatgagc tgacctgcgg aacctgtcaa ggctatcgtc tcaatgacca ggccttgtct
    31501 gtccgtgtgg gcggccagca agggccacat attggagaaa tctcagacct gtctatcgct
    31561 gaccacttgg acttggtgag ccagttaact ttgtctgaaa atgaagccat cattgctcgt
    31621 cccattctca aggaaatcaa ggatcgtttg accttcctta ataatgtggg tcttaactat
    31681 ctaaccctgt cacgttcagc aggaaccctt tcaggtgggg aaagtcagcg cattcgtttg
    31741 gcaacccaga ttggttccaa cctatcaggc gtcctctata tcctagacga gccgtcaatc
    31801 ggtcttcacc agagagacaa tgaccgcctg attgccagtc tgaaaaagat gcgtgacttg
    31861 ggcaatactc ttatcgtggt ggaacacgac gaagatacca tgcgtgaagc tgattatctg
    31921 attgacgttg gtcctggtgc cggtgttttt ggtggtgaga ttgttgcagc aggtacacct
    31981 aagcaagtgg ctcgcaacag caagtctatc acaggtcagt acttgtcagg caaacgtgtc
    32041 atcccagtac cagaagagcg ccgtgtcgga aatggtcgtt ttattgaagt gataggagcg
    32101 cgtgagaaca acttgcaaaa tgtcacggct cgctttccac taggaaaatt tatcgcagtg
    32161 acaggtgtgt cgggttcagg gaaatcgact ctaatcaaca gcattcttaa aaaagccatt
    32221 gcccagaagc tcaaccgtaa ttcagacaaa cctggtaagt ttaagactat tacagggatt
    32281 gagcatgtcg accgtttgat tgatattgac cagagcccta tcggacggac gccgaggtct
    32341 aacccagcta cctatacagg agtttttgac gatatacgtg acctttttgc tcagacaaat
    32401 gaagccaaga ttcgaggcta caagaagggg cgcttcagtt tcaacgtcaa gggaggtcgc
    32461 tgtgaagctt gctcaggtga cgggattatc aagattgaga tgcacttctt gccagatgtt
    32521 tatgtggctt gtgaagtttg ccacggaacc cgctataaca gtgaaaccct agaagttcac
    32581 tacaaggaaa agaatatttc gcaggtcttg gatatgacgg tcaacgatgc ggtagaattt
    32641 ttccaacaca ttccgaaaat tcaacgcaaa ctccagacca tcaaggatgt agggctaggc
    32701 tatgtgacct tgggacagcc agctaccacc ctttctgggg gagaagccca acgtatgaag
    32761 ttagctagcg aactccacaa acgctcgaca ggaaaatctt tctacattct ggatgagccg
    32821 acgacagggc ttcatacaga ggacattgct cgcctgctca aggttttagc tcgctttgta
    32881 gacgatggca atacagtcct cgtcatcgag cacaatctgg atgttatcaa gacggcagac
    32941 catatcattg acttgggacc tgagggcggt gtcggtggtg gaaccatcat cgtaacagga
    33001 actccagaag aagtagctgc caatgaagcc agctatacag gacactattt gaaaggaaag
    33061 ttacatcatg aataaacgcg tacaagcatt tctagctaaa atgcaagaaa aagaactaga
    33121 tggtatcatc atcaacaatc ttaaaaacgt ctattatttg actggttttt ggggctcaaa
    33181 cggaacagtc tttatcagcc gtgaccgtca ggtcttagtg acagactctc gctatatcat
    33241 cgcagctaag caagaaacca gtggttttga gattgtggct gatcgtgatg aattggctgt
    33301 cattgcagga attgttaagg acatgggctt gactcgtatc ggttttgaag atgagatttc
    33361 agtatcttat taccaccgta tgcaggcagc ttttgcaggt ttggacttgc ttccacaaac
    33421 tcaatttgtg gaaggtcttc gtatgattaa ggatgaggca gagattgcag cgattcgcaa
    33481 ggcttgttct atctcagacc aagctttccg cgatgcgctt gactttatca aaccaggaaa
    33541 aactgaaatt gaaattgcca acttccttga tttccgcatg cgtgagttgg gagcatctag
    33601 cttatctttt gatacgatcc tagctagcgg tatcaattct tctaaacccc atgcccatcc
    33661 aatgcacaaa ccagtggagt tgggagaagc cattaccatg gacttcggct gtctctatga
    33721 ccactatgtc agtgatatga cccggactat ctatctaggg catgttagcg atgagcaggc
    33781 agagatttac aatacggttc taaaagctaa ccaagccttg attgaccaag ctaaggcagg
    33841 cttaggtttc cgtgactttg acaaaatccc tcgtgatatt atcattgagg caggttatgg
    33901 cgactacttt actcacggca ttggccacgg tattggtctg gatatccatg aggaacccta
    33961 ctttagccag acttctacag aaactattaa gacaggtatg gccttgaccg atgaaccagg
    34021 tatctatatc gaaggcaaat atggcgttcg tatcgaggat gatatcctga ttacagagac
    34081 aggttgtgaa ttattgaccc tagctccaaa agagttgata gtcatttagt tgtttgtcaa
    34141 gaaaaaagta cgaaaatgac gaaaaaagtc aaaaaaaatt aaaaataggt cgcaagtcgg
    34201 atgtttttta tggtataata gactaaactg atagtaacat gtagcgaaag gggtaggtac
    34261 atgattaaaa tttatacagt ctcaagttgt actagctgta aaaaagcaaa aacctggctc
    34321 aatgcccacc agttaagtta taaagaacaa aaccttggta aagaaggaat tacgagagaa
    34381 gaattactgg atattctaac caaaacagat aacggaatag ccagcatcgt ttcgtctaaa
    34441 aatcgctatg ccaaagccct tggagtggat attgaagatt tgagtgtcaa tgaagttctc
    34501 aatctgatta tggaaacacc gagaatttta aagagcccaa tccttgtaga tgaaaaacgc
    34561 ctgcaagttg gctacaagga agacgatatt cgtgccttcc taccacgctc tgtccgtaat
    34621 gtagaaaatg cagaagcacg tttacgtgca gctctataaa catcaaggct gggagcactt
    34681 cccagtctta ttctatttta atttcaaaaa gaaagaagag agaaatgaaa aaaatagttc
    34741 ttgttagtct agctttcctt tttgtcctgg ttggttgcgg acagaaaaaa gaaactggaa
    34801 cagctacaaa aacagaaaag gatacgcttc agtcggcatt gccagttatt gaaaatgccg
    34861 agaagaatac agttgtaacc aagactttgg tcttgcccaa gtcagatgat ggtagccagc
    34921 aaactcaaac cattacatac aaggacaaga cttttttgag cttaacgatt caacaaaaac
    34981 gtccagtttc tgatgagttg aagatttata tcgaccaaca tggagtggaa gaaactcaaa
    35041 aagctcttct tgaagcggag gagaaggata agtctatcat tgaagctcgt aaattggcag
    35101 gtttcaaact tgaaacaaaa ctattgagcg caacggaact tcaaacaacg actagttttg
    35161 attttcaagt tctggatgtc aagaaggctt cccagttgga acatctgaag aatattggtt
    35221 tggaaaatct tttgaaaaat gaaccaagca aatatatttc agatagattg gcaaatggcg
    35281 cgacagaaca atagaaaatc caaataaata gactgcctga attgtagaag gtaaggaatt
    35341 atgctataat ggtactattc taaggaaaga gggtgttaga atgggattta ctgaagaaac
    35401 agtacgtttt aaattggacg attccaataa aaaagaaatt agcgaaactt tgacagatgt
    35461 ttatgcttcg ttgaacgata agggttacaa cccaattaac caaatcgtag gttacgtatt
    35521 gagtggagac cctgcctacg ttcctcgtta taataatgca cgaaatcaaa tccgtaagta
    35581 tgagcgtgat gaaatcgttg aggaattggt tcgctactac ctaaaaggac aaggagtcga
    35641 tctataacct atgagaatta tgggattgga cgtcggttca aaaacggtag gggtggcgat
    35701 tagcgatccg cttggtttta cagctcaagg gcttgaaatc atccagataa atgaagaaca
    35761 aggccaattt ggttttgacc gcgttaagga attggttgat acttacaagg tggaacgatt
    35821 tgtagtgggc ttgcctaaaa acatgaacaa tacaagtgga ccgcgcgtag aagctagtca
    35881 agcctacgga gcaaagctag aagagttttt tggtttacca gtagactatc aggatgaacg
    35941 cttgacaaca gtggctgctg agcgcatgtt gattgaacaa gcagatatca gtcgcaataa
    36001 gcgcaagaaa gtcattgata agttagcagc tcagctgatt ttacaaaatt atttagatag
    36061 aaaattttaa tataaaggag aggctatgtc acacgatcat aaccacgacc acgaagaacg
    36121 tgaactaatc acactagtag atgagcaagg aaatgaaacc ttgtttgaaa tccttttgac
    36181 aattgatgga aaagaagaat ttggtaaaaa ctatgttctt ctagtaccag ttaacgcaga
    36241 agaagacgaa gacggacaag ttgaaatcca agcttactca ttcattgaaa atgaagatgg
    36301 aacagaaggc gaattgcaac caatcccaga agactcagaa gacgaatgga acatgattga
    36361 agaagtcttc aacagcttta tggaggagta aaaacgtccg ggggacgttt ttagccctgc
    36421 gctagaaatt agaaacgcag gtagtctggg agactgtatc agcccaactc cagaaatggg
    36481 gaagagttgg aacgtccagt gaacattttt accccatatt cacattcata gtagctagtt
    36541 attagctaac caggtaagag gtcgggacga aaatcctggc cttgttttta ttagtaacca
    36601 tactttaatg cgataattga ttggactttt gtcaaggaga tgtgtatgtt tgaagtagaa
    36661 gaatggctcc atagtcggat tggtttgaat tttcgatcag gtttgggccg aatgcagcaa
    36721 gcggtggatt tgttaggaaa tcctgagcag tcttacccta ttatccacgt aacagggact
    36781 aatgggaaag gatctaccat tgcttttatg agggaattat ttatggggca tggcaaaaaa
    36841 gttgcgacct ttacctcccc tcatatcgtc tctatcaatg accgaatctg cattaatggg
    36901 caacctatag cagacgcaga ctttatccgt ttgactgatc aggtcaagga gatggagaaa
    36961 acgcttctgc aaactcctgc ccagttgtcc ttttttgaat tgctgacctt agttgctttt
    37021 ctttatttta gggagcaaga ggtggatttg gttttattag aagtgggaat tggtggctta
    37081 cttgacacga ccaatgtggt aactggagag cttgctgtca tcacctccat tgggcttgac
    37141 catcaagaaa ccttgggtga tagtctagaa gcaattgcag agcagaaagc tggtattttc
    37201 aaggctggta agaaggcagt gattgcgaaa ttgcctccag aagctaggct tgtctgtcag
    37261 aaaaaagccg aatctttagc tgttaacctt tatcaggcag gtcaagattt tttaatgctg
    37321 aatggtgatt tttcaagctc tttactaaat ctttcacagc tgaacatagg cttagaagga
    37381 gtctatcagc aggagaatgc agccttggcg ttgcaaactt ttcttctttt tatgggagaa
    37441 ggaaaggaag ctgttgatga acaggctgta agaaaggcct tggaacagac ccattgggct
    37501 ggtcgcttgg agcgtattcg cccacagatt tacttggatg gtgctcataa cctccctgcc
    37561 ttgactcgct tggttgagtt tatcaaagaa aaagagcagg aaggctatcg acctcaaatc
    37621 ctttttggag ccttgaaacg gaaggattat caagggatgt taggttacct gactgaaaaa
    37681 ttgcctcagg tggaactcaa ggtgaccggc tttgactatc agggggcttt ggacgaaagg
    37741 gatgtgacag gttacgatat agtttcttct taccgagaat ttatcagcga ttttgaagaa
    37801 agagcagatg ctcaggattt gctgttcgtt acagggtctc tctattttat ctcagaagta
    37861 cggggctacc tgctggaccg tgagcagata aattgacccc cttgtttgaa cttgttatac
    37921 taggggaatt gctggttaga gaaaatttct ctaaattggt agcagaaagg aaattcatca
    37981 tgaaattaaa aagattcaca ctttctcttg cttctctagc aagttttagt ctcttagtag
    38041 cttgttcaca aagagctcaa caggttcaac agcctgttgc tcagcagcag gtccaacagc
    38101 ctgctcaaca gaataccaat actgcaaatg caggaggtaa ccaaaatcaa gcggctccag
    38161 tacaaaacca acctgttgct caaccgaccg atattgatgg gacttatact ggtcaggatg
    38221 acggagaccg tatcacttta gtggtaactg gaacgactgg tacatggact gagctcgaat
    38281 ctgacgggga tcagaaagtc aaacaggtta catttgattc agcaaatcaa cgcatgatta
    38341 ttggcgatga tgtcaaaatt tacactgtaa acggtaatca aatcgtcgta gatgatatgg
    38401 atagagaccc atcggaccaa atcgttttaa ctaaataaga ctatgcaagt agaaagggct
    38461 ggatttttca gcctttttac ttttacctag aaaataatca taaatacttg ccttctaccg
    38521 aatacctgcg ttatactagt atcaattact tgtttttagc attcaaacta tcaaggaggg
    38581 gatatgaaat ataggaaatt tcaattattg atgtccaagt atggctttag tctttcgatt
    38641 atgctacttg aactttgtct tgtttttggt ctctttcttt atttaggacg catggctccc
    38701 attttatgga ttactgtcct cattctactg agtatcatca caatcatttc gatagtcaac
    38761 cgtaatacga ctcctgagaa taaggtaacc tggttgttag tagcctttgt gccagtattt
    38821 ggtcccttgc tctatctgat gtttggtgaa aggcgattgt ccaaaaaaga aatcaaacaa
    38881 ctgaagaagc taggctctat gcatttccaa gaagcaaata gccagctact aaaagagaaa
    38941 ttaaaagaaa gtgacaaggc agcttatgga gtcatcaagt ccttattgag tatggatacc
    39001 aatgctgaca tctatgatca aactgcctct acattttttc ctaacggaga agctatgtgg
    39061 aaaaagatgg tagaagatct taaaaaggct gagaaattta ttttcttgga atattacatt
    39121 atagaagaag gtttgatgtg gaatcgcata ctagatatac tagagcaaaa ggtagctcag
    39181 ggtgtagagg ttaagatgct ctatgatgat atcggctgta tggctacttt aacaggagat
    39241 tatgcacatc gacttcgtca gctgggcatc gaggcccata aattcaataa agttattcct
    39301 cgtttgacag tggcttataa taacagagat catagaaaaa tattgattgt tgatggtcag
    39361 atagcctata ctggtggggt caatctggca gatgagtaca ttaaccacgt cgagagattt
    39421 ggttattgga aggatagtgg aattcgctta gacggactag cagtaaaagc tctgacacgc
    39481 ttatttttga ccacttggta cattaatcga ggagaaatta gtgattttga tcaatatcat
    39541 ttagaaaatc attctatccc gagtgacggt ttaaccattc catacggaag tggacccaag
    39601 ccaatttttc gagcgcaggt agggaaaaaa gtttatcaga gtttaatcaa tcaagcaaca
    39661 gaatcggtct atattacgac accttatttg attatagatt atgatttaac agagacaatc
    39721 aaaaatgcag ctatgagagg ggtcgatgtt cgaattatca ccccttacat accagataag
    39781 aagttcattc agttagtcac gagaggagct tatcccgacc ttctttctgc tggtgttcgg
    39841 atttatgagt atagtccagg ttttattcat agtaagcaga tgttggtaga cgaagatttt
    39901 gcggtggtgg ggacaatcaa tctcgactac cggagcttgg tacaccatta tgaaaatgca
    39961 gtcttactct ataaaactcc ttctataagg gaaatcgccc gagattttcg aaatatattt
    40021 gcagattctc aggaagtcta tcctcattct atcaaaacga gctggtatca aaagcttgta
    40081 aaagaaatcg cccagctatt cgcccctatc ttataagaaa tctagaactt aggatacaaa
    40141 ccagacctat ttttcttgtc ttttacgaat agaaagaaag aggaggggaa ttcatgctaa
    40201 ctaaggaaga agtgaatgat atgattgagt tcaaacggac acatttacac cgctttcggg
    40261 aaattgaaac ctttgtgaaa ctatgcaaaa aatcactcaa acagccatcg caagctgaca
    40321 atcctaggac tttttgatat actaagacag ataaattgaa caggagaaac gatatgagtg
    40381 tgtatggtag agtagaagaa gttcataagg agaatcgtga acctctagaa taccaaatcg
    40441 aacaagaatc gcatcatcgt gaatcaagtc gtcttccttt ggtgaaaatt ttactatgga
    40501 gtacgcttgt aacggggata actctaggag taccgctatt actcgactta atgagtgcac
    40561 aagaagtgca ggatttttat gcaggttggg cgcttcatca gacagggaag atttacagcg
    40621 actattatgg aagtcaaggt ttgctttatt atttgctgac ttacgtgagt cagggcggat
    40681 ttttctttgc catctttgag tggttagcct tggtagcagg aggatttttc ctttttagat
    40741 cagcggacac cttgacagag caaggagacc aagctggaca tctggtgact attttttaca
    40801 tgctagttac aggtcttgct tttggtggag gctatgcgac tcttttagcg cttcctttct
    40861 tattcgcagc ctttagttta gttgcggctt acctaagcaa tccaagccat gataagggat
    40921 ttgtacggat tgggctagct ttggcaggcg gatttttctt tgctccctta tcatcgctcc
    40981 tgtttattgc tgtagtgagt ttaggcttgt tggtctttaa ccttgggcat agacgttttg
    41041 tgcatgggtt ttatcagttt cttgcagtgg ctttaggttt ttcacttgtc ttttatccaa
    41101 ctgcctacta tagtgctgca acaggaagtt ttggggatgc gattagtggt attcgttatc
    41161 ctattgacag tattcgcttt gattttactt ctaaaatttt agagaatatg tttttttatg
    41221 gcttgttgtc cattggtttg ggatttgtgg tttgtatctt tttaggtctc tttcaatcca
    41281 aaccctttaa actatacgtc atttcggttc ctgcaagtct tgtggtaatt ttaggtttga
    41341 ttttactttt cttttcacaa gagcctctgc acgcttctta tttgatggtc gtcttccctg
    41401 ttttcctact tttattggta accaatatta agagtcaaca gagggggcgt agtgctagaa
    41461 gaagccgaag agaaacgcca gttagcctat ggagtcgttt cttcaaagga aatctatatc
    41521 tgctagtttt tgggtttgtc tatcttttgt ctgttccttt tttgatgaag tttgtccttt
    41581 atccagtacc ttatcaagaa cgtaatcgtc ttgctgattt ggtaaaagag gagacaaata
    41641 cggaagatgc tatctatgca tgggatgata ctgcgactct ttatcgtaag agtgagcgct
    41701 tgtcgccatc ggcgattttg tccccgttgc actatacagc aactgaagaa aatcgtaata
    41761 agttacttaa tgacttgaaa gaaaaacaac ctaaggtgat tgtggtaaat gacaaggtgg
    41821 tagtctggtc tgaagtggaa acactcttaa aagaaaatta ccaacaagta aagactgatt
    41881 actcagagtt taaagtctat aaaattaaat aaccaaatca atatcttgtg tatttttaaa
    41941 aattttagga tttttaacac aagatattga tttttctttt tagagtggta taatactttt
    42001 tagaaagaac attttagaaa agagcatgca tatgattgca ctagaagaaa aaattacaat
    42061 tttgccaact ctcttcgtcg agaaacgaga tgggagacgt gttgtatttg atgtggacaa
    42121 gattgacaag gctctccaca aggcggctga caaggttatg gatgtgacac ccttggttga
    42181 aaaacgcctc aatgatctga ctgagcgaat tattacagaa attcatagtc gctttccaca
    42241 gggaattaag atttacgaaa ttcaaaatat cgtagaacat gaactccttg aagccaaaga
    42301 atatgcgctg gctgaggagt atattactta tcggacacag agggattttg agcgctcaaa
    42361 agcgacggat atcaacttta gtattcataa acttctcaac aaagaccaga cagttgtcaa
    42421 tgaaaatgct aataaagaca gtgatgtctt taacactcag cgtgatttga cagcagggat
    42481 tgttgggaaa tcaatcggac tgcaaatgct tcctaagcac gtagccaatg cccaccaaaa
    42541 gggggatatc cactatcacg atttggacta cagtccctat acccctatga ccaactgctg
    42601 tttgattgat tttaagggta tgttggaaaa tggttttaag attggaaatg cagaggtaga
    42661 gagtcccaag tctatccaga ctgcgacagc acagatttct caaatcattg ccaacgttgc
    42721 ttctagccag tacggtggct gttcagctga ccgtatcgat gaaattttgg cgccttatgc
    42781 agagaagaac tatcaaaaac atctcaaaga tgcagaagag tgggtattgc ctgaaaaacg
    42841 ggaagattac gcttggaaga aaacgcaaaa ggacatctac gatgccatgc aatctcttga
    42901 gtatgagatc aatactctct tcacttcaaa tggacaaaca ccttttactt cgttaggttt
    42961 tggtctggga accagtcgtt ttgaacgaga aattcaaaaa gctattttaa acattcgcat
    43021 caagggtctt ggttcagaac accgtacggc tatctttcct aaacttattt tcactctgaa
    43081 aagaggcctt aatttagagg aaggaactcc caactacgac atcaagcagt tggctcttga
    43141 gtgtgcaacc aaacggatgt atccagatgt tttatcttac gataagattg ttgacttgac
    43201 aggatccttc aaggtgccta tgggttgccg ttctttcctc caaggatgga aggatgaaaa
    43261 tggtgtagaa gtcaattcag gtcgcatgaa tctgggtgtt gtgacggtta atctgcctcg
    43321 tattgctctt gagtctgaag gtgatatgaa taagttctgg gaaatcttca acgagcgaat
    43381 gaatatcgca gaagatgctc ttgtttaccg tgtcgaacgc actaaagagg cgacaccagc
    43441 gaatgctcct attctttatc agtacggtgc ttttggccat cgtctaggta aagaagaaag
    43501 tgttgaccag ctctttaaga atcgtcgtgc gaccgtttcg ctgggctata tcggcttgta
    43561 tgaagtagcg acagttttct ttggtaacag ctgggaaagt aatccagatg ctaaggaatt
    43621 cacgctagac atcattcacg atatgaaacg ccgtgtagaa gagtggtcag accaatatgg
    43681 ctaccatttc tctatctact caacaccatc cgaaagtctg acagaccgtt tctgccgact
    43741 agatatagac aagtttggct ctattcctga tatcacagac aaggaatact acaccaactc
    43801 tttccactac gatgttcgta aaaatccaac accgtttgaa aaattggact ttgagaaagt
    43861 ctatccagaa gcaggtgcgt caggtggttt catccattat tgtgagtatc cagtccttca
    43921 gcaaaatcca aaggccttgg aagctgtctg ggattatgct tatgaccgtg taggctatct
    43981 aggcaccaat actccgattg accgttgcta caagtgtgac tttgaagggg attttgaacc
    44041 aactgagaga gggtttgctt gtccaaactg tggcaatagc gatcctaaaa cagtagacgt
    44101 tgttaaacgg acttgtggct atcttggcaa tcctcaagcg agaccgatgg ttaacgggcg
    44161 tcacaaggaa atcgctgcgc gtgtcaaaca tatgaatggt tcaacgatta aaatagctgg
    44221 gcatcaagta acaaattaga aagaaatgaa atgggaaaat atcaactaga cgataagggg
    44281 cgcgcacaag tgacccgtta tcacgagaaa tactctaaag gtggagctgg taagaaagaa
    44341 cgcttgctta gcttcagaga acaattttta aacaagaaca agaaaaaata aaagtgagag
    44401 ccagctctcg cttttctcat agtgggaggt agggatggaa ttacgcagac caagattagc
    44461 ggataagaaa gctgttttag atatgatgac agagtttgaa aagagccaat cagcccatga
    44521 tggaggattt tgggatacag agaactttgt gtatgaagag tggttggaaa gcaatcagga
    44581 acaggaaatg gggattaatc tgcctgaagg atgggttcct gcaattcagt tagtggcttt
    44641 ttctgagaaa ggtcaagcag ttggatttct taatctccgg ttgcgcctca gtaactttct
    44701 actagaagaa ggtgggcaca ttggctactc catccgtcca tctgaaagag gcaagggtta
    44761 tgcaaaagag actctccgtc agggcttgca agttgctaag gaaaagaaca tcaagaaagc
    44821 tctggtgacc tgtagcgtga ataatcctgc tagcagagca gtcattctag caaatggtgg
    44881 aatatttgag gatgctcgca atggagtcga gcgttattgg atagaggtag cgaatgaata
    44941 atccaaaacc acaagaatgg aaaagcgagg aacttagtca aggtcgtatc attgactaca
    45001 aggcctttaa ctttgttgat ggcgaaggcg tgcgtaactc tctctatgta gcaggctgta
    45061 tgtttcactg cgagggatgt tataatgttg cgacttggtc ttttaatgct ggcattccct
    45121 atacagcaga attagaagag cagatcatgg cagatcttgc tcagccctat gttcaaggtt
    45181 tgactttgtt gggaggggag ccttttctca atactggcat tctcttgcca cttgttaagc
    45241 ggattcggaa agaattgtca gacaaggaca tctggtcctg gaccggctac acttgggaag
    45301 aaatgatgtt ggaaactcca gataaactgg aactcttgtc actgattgat attcttgtcg
    45361 atggaagata tgatcgaact aagagaaatc ttatgctcca gtttcgaggt tcgtctaacc
    45421 aacgaattat cgatgtgcaa aaatcgctca aaagtgggca agtagtgatt tgggacaagc
    45481 tcaatgacgg aaaagaaagc tatgaacagg tgaagagaga atgaagaaaa aagacttagt
    45541 agaccaacta gtctcagaga tcgagacggg gaaagtcagg acactgggaa tatacggtca
    45601 tggagcttca ggtaaatcaa cccttgcaca ggaattgtcc caagctttag attctactac
    45661 agtaaatttg ctagaaacag atccctatat cacctccgga cgctatttgg tagtacccaa
    45721 ggacgcgccg aatcaaaagg tgacagccag tctgccagtg gcgtatgaac tggagagttt
    45781 gcagagagat atccttgcct tgcaggcggg tatggatgtc ttgacaattg aagaaccttg
    45841 gaaggctagt gaagtcttgt ctggagccaa accaattttg attgtcgaag ggatgtctgt
    45901 tggttttcta cccaaggaac tctttgaaaa aaccatctgt ttctacacgg atgaggagac
    45961 cgaattaaag cgacgctttg ctagagatac gactgtgaga aatcgcgatg catcctttat
    46021 attggctagc catcagatga gacgggagca gtatctgcga tactataaag aaactgagtc
    46081 taaggcggat atcttagtgg accaatcaga agataaattt gatgtcaaga ggacttaact
    46141 tatgtataga agaaaatccc taattttaga aagaaaaagt aggaaaacag tttcatccca
    46201 aaaaaacgaa aaaacctaac aaatcccttg caatcgcagg ggatttgtgt tattctatta
    46261 tggtgctgta aattacagcc ttagttttga tgcaagaggt tgcgacacgc tcggttgcat
    46321 tgccacgcaa caccgcgtcg gttttcttgt ggagctagcc tattatctta aatagacgaa
    46381 aaggagaaaa agatggcaaa caaaaaaatc cgtatccgtt tgaaagctta cgaacaccgt
    46441 acgcttgaca cagcggctgc aaaaatcgta gaatcagcta ctcgtacagg tgcacaagtt
    46501 gcgggtccaa tcccacttcc aactgaacgt agcctctaca caatcattcg tgcgactcac
    46561 aaatacaaag actctcgcga acaatttgaa atgcgtacac acaaacgttt gatcgatatc
    46621 gttaacccaa ctcaaaaaac agttgatgcc ttgatgaaat tggatcttcc aagtggtgta
    46681 aacgtagaaa tcaaacttta atctaaaata taaaagagca gaggctggtg tttcaatcta
    46741 attgaacacg ggctaaactc ggtgtgaaaa agataaactt cctagtgtct gctagacact
    46801 gcgtcagttt cctattttca cgttgagttt gacgcccttt gtatcttaga cttgagcata
    46861 aaaaacgctc gttaaaaact ttttgaataa aaatatagaa aaggaactat tttctcatga
    46921 caaaaggaat cttagggaaa aaagtgggaa tgactcaaat cttcactgaa gctggcgaat
    46981 tgatccctgt aacagttatt gaagcaactc caaacgttgt tcttcaagtt aaaactgttg
    47041 aaacagacgg atacaacgct atccaagttg gtttcgatga caaacgcgaa gtattgagca
    47101 acaaacctgc taaaggacat gtagcgaaag ctaacacggc tcctaagcgc ttcattcgtg
    47161 aattcaaaaa cgttgaaggc ttggaagttg gtgctgaaat tacagttgaa acattcgcag
    47221 ctggagacgt tgttgacgta actggtactt ctaaaggtaa aggtttccaa ggtgttatca
    47281 aacgccacgg acaatcacgt ggaccaatgg ctcacggttc tcgttaccac cgtcgtccag
    47341 gttctatggg acctgttgca cctaaccgcg tattcaaagg taaaaacctt gcaggacgta
    47401 tgggtggtga ccgcgtaaca attcaaaatc ttgaagttgt acaagttgtt ccagaaaaga
    47461 acgttatcct tatcaaaggt aacgtaccag gtgctaagaa atctcttatc actatcaaat
    47521 cagcagttaa agctggtaaa taataaagaa aggggaaatc agtcacaatg gcaaacgtaa
    47581 cattatttga ccaaactggt aaagaagctg gccaagttgt tcttagcgat gcagtatttg
    47641 gtatcgaacc aaatgaatca gttgtgtttg atgtaatcat cagccaacgc gcaagccttc
    47701 gtcaaggaac acacgctgtt aaaaaccgct ctgcagtatc aggtggtgga cgcaaaccat
    47761 ggcgtcaaaa aggaactgga cgtgctcgtc aaggttctat ccgctcacca caatggcgtg
    47821 gtggtggtgt tgtcttcgga ccaactccac gttcatacgg ctacaaactt ccacaaaaag
    47881 ttcgtcgcct agctcttaaa tcagtttact ctgaaaaagt tgctgaaaac aaattcgtag
    47941 ctgtagacgc tctttcattt acagctccaa aaactgctga atttgcaaaa gttcttgcag
    48001 cattgagcat cgattctaaa gttcttgtta tccttgaaga aggaaatgaa ttcgcagctc
    48061 tttcagctcg taaccttcca aacgtgaaag ttgcaactgc tacaactgca agtgttcttg
    48121 acatcgcaaa tagcgacaaa cttcttgtca cacaagcagc tatctctaaa atcgaggagg
    48181 ttcttgcata atgaatttgt atgatgttat caaaaaacct gtcatcactg aaagctcaat
    48241 ggctcaactt gaagcaggaa aatatgtatt tgaagttgac actcgtgcac acaaactttt
    48301 gatcaagcaa gctgttgaag ctgctttcga aggtgttaaa gttgccaatg ttaacacaat
    48361 caacgtaaaa ccaaaagcta aacgtgttgg acgttacact ggttttacta acaaaactaa
    48421 aaaagctatc atcacactta cagctgattc taaagcaatc gagttgtttg ctgctgaagc
    48481 tgaataatct aaggaggaaa tatcgtggga attcgtgttt ataaaccaac aacaaacggt
    48541 cgccgtaata tgacttcttt ggatttcgct gaaatcacaa caagcactcc tgaaaaatca
    48601 ttgcttgttg cattgaagag caaggctggt cgtaacaaca acggtcgtat cacagttcgt
    48661 caccaaggtg gtggacacaa acgtttctac cgtttggttg acttcaaacg taataaagac
    48721 aacgttgaag cagttgttaa aacaatcgag tacgatccaa accgttctgc aaacatcgct
    48781 cttgtacact acactgacgg tgtgaaagca tacatcatcg ctccaaaagg tcttgaagta
    48841 ggtcaacgta tcgtttcagg tccagaagca gatatcaaag tcggaaacgc tcttccactt
    48901 gctaacatcc cagttggtac tttgattcac aacatcgagt tgaaaccagg tcgtggtggt
    48961 gaattggtac gtgctgctgg tgcatctgct caagtattgg gttctgaagg taaatacgtt
    49021 cttgttcgtc ttcaatcagg tgaagttcgt atgattcttg gaacttgccg tgctacagtt
    49081 ggtgttgtcg gaaacgaaca acatggactt gtaaaccttg gtaaagcagg acgtagccgt
    49141 tggaaaggta tccgcccaac agttcgtggt tctgtaatga accctaacga tcacccacac
    49201 ggtggtggtg aaggtaaagc accagttggt cgtaaagcac catctactcc atggggcaaa
    49261 cctgctcttg gtcttaaaac tcgtaacaag aaagcgaaat ctgacaaact tatcgttcgt
    49321 cgtcgcaacg agaaataata ttaaactagt cgcttaagca actagtaaat ccgccagctc
    49381 ggtagcgctc cataggagtg caagccgctg tggtacaaca tttaaaggag aaaatataaa
    49441 aatgggacgc agtcttaaaa aaggaccttt cgtcgatgag catttgatga aaaaagttga
    49501 agctcaagct aacgacgaaa agaaaaaagt tattaaaact tggtcacgtc gttcaacgat
    49561 cttcccaagt ttcattggtt acactattgc agtttatgac ggacgtaaac acgtacctgt
    49621 ttacatccaa gaagacatgg taggccacaa acttggtgaa tttgcaccaa ctcgtactta
    49681 caaaggtcac gctgcagacg acaagaaaac acgtagaaaa taaggagaac ataaatggca
    49741 gaaattactt cagctaaagc aatggctcgt acagtacgtg tttcacctcg taaatcacgt
    49801 cttgttcttg ataacatccg tggtaaaagc gtagccgatg caatcgcaat cttgacattc
    49861 actccaaaca aagctgctga aatcatcttg aaagttttga actcagctgt agctaacgct
    49921 gaaaacaact ttggtttgga taaagctaac ttggtagtat ctgaagcatt cgcaaacgaa
    49981 ggaccaacta tgaaacgttt ccgtccacgt gcgaaaggtt cagcttcacc aatcaacaaa
    50041 cgtacagctc acatcactgt agctgttgca gaaaaataag gaggtaaaat cgtgggtcaa
    50101 aaagtacatc caattggtat gcgtgtcggc atcatccgtg attgggatgc caaatggtat
    50161 gctgaaaaag aatacgcgga ttaccttcat gaagatcttg caatccgtaa attcgttcaa
    50221 aaagaacttg ctgacgcagc agtttcaact attgaaatcg aacgcgcagt aaacaaagtt
    50281 aacgtttcac ttcacactgc taaaccaggt atggttatcg gtaaaggtgg tgctaacgtt
    50341 gatgcactcc gtgcaaaact taacaaattg actggaaaac aagtacacat caacatcatc
    50401 gaaatcaaac aacctgattt ggatgctcac cttgtaggtg aaggaattgc tcgtcaattg
    50461 gagcaacgtg ttgctttccg tcgtgcacaa aaacaagcaa tccaacgtgc aatgcgtgct
    50521 ggagctaaag gaatcaaaac tcaagtatca ggtcgtttga acggtgcaga tatcgcccgt
    50581 gctgaaggat actctgaagg aactgttccg cttcacacac ttcgtgcaga tatcgattac
    50641 gcttgggaag aagcagatac tacatacggt aaacttggtg ttaaagtatg gatctaccgt
    50701 ggtgaagttc ttccagctcg taaaaacact aaaggaggta aataaccaat gttagtacct
    50761 aaacgtgtta aacaccgtcg tgaattccgt ggaaaaatgc gcggtgaagc aaaaggtgga
    50821 aaagaagtag cattcggtga atacggtctt caagctacaa ctagccactg gatcactaac
    50881 cgccaaatcg aagctgctcg tatcgccatg actcgttaca tgaaacgtgg tggtaaagtt
    50941 tggattaaaa tcttcccaca caaatcatac actgctaaag ctatcggtgt gcgtatggga
    51001 tctggtaaag gggcacctga aggttgggta gcaccagtta aacgtggtaa agtgatgttc
    51061 gaaatcgctg gtgtatctga agagattgca cgtgaagcgc ttcgacttgc tagccacaaa
    51121 ttgccagtta aatgtaaatt cgtaaaacgt gaagcagaat aaggagaagg catgaaactt
    51181 aatgaagtaa aagaatttgt taaagaactt cgtggtcttt ctcaagaaga actcgcgaag
    51241 cgcgaaaacg aattgaaaaa agaattgttt gaacttcgtt tccaagctgc tactggtcaa
    51301 ttggaacaaa cagctcgctt gaaagaagtt aaaaaacaaa tcgctcgtat caaaacagtt
    51361 caatctgaag cgaaataata gactagggaa ggagaaattt caatggaacg caataatcgt
    51421 aaagttcttg ttggacgtgt tgtatctgac aaaatggaca agacaatcac agttgtagtt
    51481 gaaacaaaac gtaaccaccc agtctatggt aaacgtatta actactctaa aaaatacaaa
    51541 gctcatgatg aaaacaatgt tgccaaagaa ggtgatatcg tacgtatcat ggaaactcgc
    51601 ccgctttcag ctacaaaacg tttccgtctt gtagaagttg ttgaagaagc ggtcatcatc
    51661 taatcaaacc tgaaaggaga aaactgaaat gattcaaaca gaaactcgtt tgaaagtcgc
    51721 agacaacagc ggtgctcgcg aaatcttgac tatcaaagtt cttggtggtt caggacgtaa
    51781 atttgcaaac atcggtgatg ttatcgtggc atctgtaaaa caagctactc ctggtggtgc
    51841 ggttaaaaaa ggtgacgttg ttaaagcagt tatcgttcgt actaaatcag gtgctcgtcg
    51901 tgctgatggt tcatacatca aatttgacga aaacgcagca gttatcatcc gtgaagacaa
    51961 aactcctcgc ggaacacgta tctttggccc agttgcacgt gaattgcgtg aaggtggttt
    52021 catgaagatc gtgtcacttg ctccagaagt actttaattt ttaggaacaa actagtcccc
    52081 tagcttcaag ctagggtgcc cttatgggcg taagaaaaat caaggagaaa cctaatgttt
    52141 gtaaaaaaag gcgacaaagt tcgcgtaatc gctggtaaag ataagggaac agaagctgtt
    52201 gtccttactg cccttccaaa agtaaacaaa gttatcgttg aaggtgttaa cattgttaag
    52261 aaacaccaac gtccaactaa cgagcttcct caaggtggta tcatcgagaa agaagcagct
    52321 atccacgtat caaatgttca agttttggac aaaaatggtg tagctggtcg tgttggttac
    52381 aaatttgtag acggtaaaaa agttcgctac aacaaaaaat caggcgaagt gcttgattaa
    52441 tcacgaagga aaggagaagt ataatggcaa atcgtttaaa agaaaaatat cttaatgaag
    52501 tagttcctgc tttgacagaa caattcaact actcatcagt gatggctgtg cctaaagtag
    52561 ataagattgt tttgaacatg ggtgttggtg aagctgtatc aaacgctaaa agccttgaaa
    52621 aagctgctga agaattggca cttatctcag gtcaaaaacc acttatcact aaagctaaaa
    52681 aatcaatcgc cggcttccgt cttcgtgaag gtgttgcgat cggtgcaaaa gttacccttc
    52741 gtggtgaacg tatgtacgaa ttcttggata aattggtatc agtttcactt ccacgtgtac
    52801 gtgacttcca cggtgtccca acaaaatcat ttgatggacg cgggaactac acacttggtg
    52861 tgaaagaaca attaatcttc ccagaaatca acttcgatga cgttgacaaa actcgtggtc
    52921 ttgacatcgt tatcgtaaca actgctaaca ctgacgaaga gtcacgtgca ttgcttacag
    52981 gccttggaat gccttttgca aaataatata ggaggtaaat ctaatggcta aaaaatcaat
    53041 ggtagctaga gaggctaaac gccaaaaaat tgttgaccgt tatgctgaaa aacgtgctgc
    53101 attaaaggcg gcaggggact acgaaggttt atctaaatta cctcgcaacg cctcaccgac
    53161 tcgtttacat aatcgttgta gagttacggg gcgcccacat tcagtttacc gcaaatttgg
    53221 tctgagtcgt atcgcttttc gcgaacttgc gcataaaggt caaattcctg gtgtaacaaa
    53281 agcatcttgg taatttaaga tatcaagggt gtcaaaactc caagtaaaaa taggaaactt
    53341 gacgaagaaa ctaaagtttc taggaaagtt tatctttttc acacagagtt tagcccgggt
    53401 tcaattgggc ttgccaattt gaacccgagc tacagctttg gcaaaaaaga ccaatttgct
    53461 ttggagcatt gcttctgcat taaattgtct atttttgctc gtgctgttac gctctttgta
    53521 tcatgtatta actagcaagt gcaacttgca aactactagt aagaggagaa aaacaaaatg
    53581 gttatgactg acccaatcgc agacttccta actcgtattc gtaatgctaa ccaagctaaa
    53641 cacgaagtac ttgaagtacc tgcatcaaac atcaaaaaag ggattgctga aatccttaaa
    53701 cgcgaaggtt ttgtaaaaaa cgttgaaatc attgaagatg acaaacaagg cgtcatccgt
    53761 gtatttctta aatacggatc aaatggtgag aaagttatca ctaacttgaa acgtgtttct
    53821 aaaccaggac ttcgtgtcta caaaaaacgt gaagaccttc caaaagttct taacggactt
    53881 ggaattgcca tcctttcaac ttctgaaggt ttgcttactg ataaagaagc acgccaaaag
    53941 aatgttggtg gtgaggttat cgcttacgtt tggtaaaatc aagatacaaa gctcgtaaag
    54001 aacaaagcaa aattaggaag ttggagaagt ttgtttacaa acaagccaac ttatctattt
    54061 tgcacagttc ttagagcgtg ttcagttcag ctcttgaact aaataagtat ctgaaccccg
    54121 tgaaaactgg ccgttctggc ctgacaattt aacaggagaa aataaacatg tcacgtattg
    54181 gtaataaagt tatcgtgttg cctgctggtg ttgaactcgc taacaatgac aacgttgtaa
    54241 ctgtaaaagg acctaaagga gaacttactc gtgagttctc aaaagatatt gaaatccgtg
    54301 tggaaggtac tgaaataact cttcaccgtc caaacgattc aaaagaaatg aaaactatcc
    54361 acggaactac tcgtgccctt ttgaacaaca tggttgttgg tgtatcagaa ggattcaaga
    54421 aagaacttga aatgcgtggg gttggttacc gtgcacagct tcaaggatct aaacttgttt
    54481 tggctgttgg taaatctcat ccagacgaag ttgaagctcc agaaggaatt acttttgaac
    54541 ttccaaaccc aacaacaatc gttgttagcg gaatttcaaa agaagtagtt ggtcaaacag
    54601 ctgcttacgt acgtagcctt cgttcaccag aaccatataa aggtaaaggt atccgttacg
    54661 ttggtgaatt cgttcgccgt aaagaaggta aaacaggtaa ataatgttga gtggttgatc
    54721 atcaaccacc aacctatttt ccaactttgt gcatagcaca cgatttaaaa ctaaagaggt
    54781 gaaaactgtg atttcaaaac cagataaaaa caaactccgc caaaaacgcc accgtcgcgt
    54841 tcgcggaaaa ctctctggaa ctgctgatcg cccacgtttg aacgtattcc gttctaatac
    54901 aggcatctac gctcaagtga ttgatgacgt agcgggtgta acgctcgcaa gtgcttcaac
    54961 tcttgataaa gaagtttcaa aaggaactaa aactgaacaa gccgttgctg tcggtaaact
    55021 cgttgcagaa cgtgcaaacg ctaaaggtat ttcagaagtg gtgttcgacc gcggtggata
    55081 tctatatcac ggacgtgtga aagctttggc tgatgcagct cgtgaaaacg gattgaaatt
    55141 ctaataggag gacactagaa aatggcattt aaagacaatg cagttgaatt agaagaacgc
    55201 gtagttgctg tcaaccgtgt tacaaaagtt gttaaaggtg gacgtcgtct tcgtttcgca
    55261 gctcttgttg ttgttggtga ccacaatggt cgcgtaggat ttggtactgg taaagctcaa
    55321 gaagttccag aagcaatccg taaagcagta gatgatgcta agaaaaactt gatcgaagtt
    55381 cctatggttg gaacaacaat cccacacgaa gttctttcag aattcggtgg agctaaagta
    55441 ttgttgaaac ctgctgtaga aggttctgga gttgccgctg gtggtgcagt tcgtgccgtt
    55501 gtggaattgg caggtgtggc agatattaca tctaaatcac ttggttctaa cactccaatc
    55561 aacattgttc gtgcaactgt tgaaggtttg aaacaattga aacgcgctga agaaattgct
    55621 gcccttcgtg gtatttcagt ttctgatttg gcataagaaa ggggataaaa tggctcaaat
    55681 taaaattact ttgactaagt ctccaatcgg acgcattcca tcacaacgta aaactgttgt
    55741 agcacttgga cttggcaaat tgaacagctc tgttattaaa gaagataacg ctgctatccg
    55801 tggtatgatt acagcagtat ctcacttagt aacagttgaa gaagtaaact aatgaagttt
    55861 taggggatgt gcactgtacc atcccctaaa actagatata gtcatctatg atgacatcgt
    55921 ataggcgagt tgatggggga gacaaccttt tctcccttat cggcgctagc attttacaaa
    55981 agaggagaaa ataaaaatga aacttcatga attgaaacct gcagaaggtt ctcgtaaagt
    56041 acgtaaccgc gttggtcgtg gtacttcatc aggtaacggt aaaacatctg gtcgtggtca
    56101 aaaaggtcaa aaagctcgta gcggtggcgg agttcgcctt ggttttgaag gtggacaaac
    56161 tccattgttc cgtcgtcttc caaaacgtgg attcactaac atcaacgcta aagaatacgc
    56221 aattgtgaac cttgaccaat tgaacgtctt tgaagatggt gctgaagtaa ctccagttgt
    56281 tcttatcgaa gcaggaattg ttaaagctga aaagtcaggt attaaaattc ttggtaacgg
    56341 tgagttgact aagaaattga ctgtgaaagc agctaaattc tctaaatcag ctgaagaagc
    56401 tatcactgct aaaggtggtt cagtagaagt catctaagag aggtgaccta tgttttttaa
    56461 attattaaga gaagctctta aagtcaagca ggttcgatca aaaattttat ttacaatttt
    56521 tatcgttttg gtctttcgta tcggaactag cattacagtt cctggtgtga atgccaatag
    56581 cttgaatgct ttaagtggat tatccttctt aaacatgttg agcttggtgt cggggaatgc
    56641 cctaaaaaac ttttcgattt ttgccctagg agttagtccc tatatcaccg cttctattgt
    56701 tgtccaactc ttgcaaatgg atattttacc caagtttgta gagtggggta aacaagggga
    56761 agtaggtcga agaaaattga atcaagctac tcgttatatt gctctagttc tcgcttttgt
    56821 gcaatctatc gggattacag ctggttttaa taccttggct ggagctcaat tgattaaaac
    56881 tgctttaact ccacaagttt ttctgacgat tggtatcatc ttaacagctg gtagtatgat
    56941 tgtcacttgg ttgggtgagc aaattacaga taagggatac ggaaacggtg tttccatgat
    57001 tatctttgcc gggattgttt cctcaattcc agagatgatt cagggcatct atgtggacta
    57061 ctttgtgaac gtcccaagta gccgtatcac ttcatctatc attttcgtaa tcattttgat
    57121 tattactgta ttgttgatta tttactttac aacttatgtt caacaagcag aatacaaaat
    57181 tccaatccaa tatactaagg ttgcacaagg tgctccatct agctcttacc ttccgttaaa
    57241 ggtaaaccct gctggagtta tccctgttat ctttgccagt tcgattactg cagcgcctgc
    57301 ggctattctt cagtttttga gtgccacagg tcatgattgg gcttgggtaa gggtagcaca
    57361 agagatgttg gcaactactt ctccaactgg tattgccatg tatgctttgt tgattattct
    57421 ctttacattc ttctatacgt ttgtacagat taatcctgaa aaagcagcag agagcctaca
    57481 aaagagtggt gcctatatcc atggagttcg tcctggtaaa ggtacagaag aatatatgtc
    57541 taaacttctt cgtcgtcttg caactgttgg ttccctcttc cttggtgtga tttccatttt
    57601 accgattgca gctaaagatg tatttggtct ttctgatgtt gttgcctttg gtggaacaag
    57661 tctcttgatc attatctcta caggtatcga aggaatcaag caattggaag gttacctatt
    57721 gaaacgtaag tatgttggtt tcatggacag aacagaataa aagtatttac tgaatcagta
    57781 aatactgagg gagtggaggt ttaaactctg acatttgtaa gagttggatc tcccctcttc
    57841 tattttgttt ttaaataggg gtgaaaagac tttttgcttc tatttaaaaa taaaataagg
    57901 agatcaaatc atgaatcttt tgattatggg cttacctggt gcaggtaagg gaactcaagc
    57961 agcaaaaatc gtagaacaat tccatgttgc acatatctca acaggtgata tgttccgcgc
    58021 tgcaatggca aatcaaactg aaatgggtgt tcttgctaag tcatatattg acaagggtga
    58081 attggttcct gacgaagtta caaatggaat cgtaaaagaa cgcctttcac aagatgatat
    58141 taaagaaaca ggattcttat tggatggtta cccacgtaca attgaacaag ctcatgcctt
    58201 ggacaaaaca ttggctgaac ttggcattga actagaaggt gttatcaata ttgaagtgaa
    58261 ccctgacagc ctcttggaac gtttgagtgg ccgtatcatc caccgcgtaa ctggagaaac
    58321 tttccacaag gtctttaacc caccagttga ctataaagaa gaagattact accaacgtga
    58381 agatgataag cctgagacag taaaacgtcg tttggatgtt aatattgctc aaggagaacc
    58441 aatcattgct cactaccgtg ccaaaggttt ggttcatgac atcgaaggta atcaagatat
    58501 caatgatgtc ttctcagata ttgaaaaagt attgacaaat ttgaaataaa gcgtttttca
    58561 cacttgcaaa aatccgctac aaatgttata ctgagatagt ctgacttata attgttgtct
    58621 ctgtgtctag aggcatcgaa tcgaaattta tggaggtgct tttgcgtggc aaaagacgat
    58681 gtgattgaag ttgaaggcaa agtagttgat acaatgccga atgcaatgtt tacggttgaa
    58741 cttgaaaatg gacatcagat tttagcaaca gtttctggta aaattcgtaa aaactatatt
    58801 cgtattttag cgggagatcg tgttactgtc gaaatgagtc catatgactt gacacgtgga
    58861 cgtatcactt accgctttaa ataatcgaaa aacttggagg gataagaaat gaaagtaaga
    58921 ccatcggtca aaccaatttg cgaatactgt aaagttattc gtcgtaatgg tcgtgttatg
    58981 gtaatttgcc cagcaaatcc aaaacacaaa caacgtcaag gataagatag aaaggagaaa
    59041 acatggctcg tattgctgga gttgatattc caaatgacaa acgcgtagta atctcattga
    59101 cttatgttta tggtatcgga cttgcaacat ctaagaaaat tttggctgct gctggaatct
    59161 cagaagatgt tcgtgtacgt gatcttacat cagatcaaga agatgctatc cgtcgtgaag
    59221 tggatgcaat caaagttgaa ggtgaccttc gtcgtgaagt aaacttgaac atcaaacgtt
    59281 tgatggaaat cggttcatac cgtggtatcc gtcaccgtcg tggacttcct gtccgtggac
    59341 aaaacactaa aaacaacgct cgcactcgta aaggtaaagc tgttgcgatt gctggtaaga
    59401 aaaaataata taggaggtaa aagtcttggc taaaccaaca cgtaaacgtc gtgtgaaaaa
    59461 gaatatcgaa tctggtattg ctcatattca cgctacattt aataacacta ttgttatgat
    59521 tactgatgtg catggtaatg caattgcttg gtcatcagct ggtgctcttg gtttcaaagg
    59581 ttctcgtaaa tctacaccat tcgctgctca aatggcttct gaagctgctg ctaaatctgc
    59641 acaagaacac ggtcttaaat cagttgaagt tactgtaaaa ggtccaggtt ctggtcgtga
    59701 gtcagctatt cgtgcgcttg ctgccgctgg tcttgaagta acagcaattc gtgatgtgac
    59761 tccagtgcca cacaatggtg ctcgtcctcc aaaacgtcgc cgtgtataat catcgcatta
    59821 cactgctttt cgtttaagag ggagtaacta aatgatcgag tttgaaaaac caaatataac
    59881 aaaaattgat gaaaataaag attatggcaa gtttgtaatc gaaccacttg aacgtggcta
    59941 cggtacaact cttggtaact ctcttcgtcg tgtacttcta gcttctctac caggagcagc
    60001 tgtgacatct atcaacattg atggtgtgtt acatgagttt gacacagttc caggtgttcg
    60061 tgaagacgtg atgcaaatca ttctgaacat taaaggaatt gcagtgaaat cgtacgttga
    60121 agacgaaaaa atcatcgaac tggatgttga aggtcctgct gaagtaacag ctggtgacat
    60181 tttgacagat agcgatattg aaattgtaaa tccagatcat tatctcttta caatcggtga
    60241 aggttcttct ctaaaagcga ctatgactgt taacagtggt cgtggatatg tacctgctga
    60301 tgaaaataaa aaggataatg caccagttgg aacacttgct gtagattcta tttatacacc
    60361 agttacaaaa gtcaactatc aagtggaacc tgctcgtgta ggtagcaatg atggtttcga
    60421 caaattaacc cttgaaatct tgacaaatgg aacaattatt ccagaagatg ctttagggct
    60481 ttcagcacgt attttgacag aacatcttga tttgtttaca aatcttactg agattgctaa
    60541 gtcaactgaa gtgatgaaag aagctgatac tgaatctgac gaccgtattt tagatcgtac
    60601 gattgaggaa ctggacttgt ctgtgcgttc atacaactgt ttaaaacgtg ccggtatcaa
    60661 tactgtgcat gatttgacag aaaaatctga agcagagatg atgaaagtac gaaatcttgg
    60721 acgcaagagt ttggaagaag tgaaactcaa actcattgat ttgggtcttg gattaaaaga
    60781 taaataaagg aggaatacat ggcttaccgt aaactaggac gcactagctc acaacgtaaa
    60841 gcaatgcttc gcgatttgac aactgacctt ttgatcaacg aatcaatcgt gacaactgaa
    60901 gctcgtgcta aagaaatccg taaaactgtt gaaaaaatga ttactctagg taaacgtggt
    60961 gatttgcatg cacgtcgtca agcagctgct ttcgtacgta atgaaatcgc atctgaaaac
    61021 tatgatgaag caactgataa gtacacttct actacagcac ttcaaaaatt gttctcagaa
    61081 atcgcacctc gttatgctga acgtaacggt ggatacactc gtatccttaa aactgaacca
    61141 cgtcgtggtg atgcagcgcc aatggcgatc atcgaattag tataaaatca tcaattttgt
    61201 tgagtgttat gatgatggag tcttgtgctc ttagtctagc tctggtctac cgctaggatt
    61261 tcggtcctag cgggaacact catcataagt tgggatagta gacgcttgtt tacgaaattg
    61321 tttttttctt aagaacaact tcgtaagcag gcgtttttga gtattttcgt tagaattatg
    61381 ctatactatt tgaaaagaat cctgtttaat gttaaggttt cttattttaa gaagaattgg
    61441 agtttactta tgaaagcgat tataactgtt gttggtaaag ataaatctgg aattgttgca
    61501 ggtgtttctg gtaaaattgc agaattagga ttgaatattg acgatatctc tcaaactgta
    61561 ttggatgaat attttacgat gatggctgtt gtatctagtg atgaaaagca agattttacc
    61621 tatcttcgta atgaatttga agcttttggg caaactttga atgtaaaaat caatattcag
    61681 agtgcagcga ttttcgaagc tatgtataat atctaggagg tcatcatgga tattagacaa
    61741 gttactgaaa ccatcgccat gattgaggag caaaacttcg atattagaac cattaccatg
    61801 gggatttctc ttttggactg tatcgatcca aatatcaatc gtgctgcgga gaaaatctat
    61861 caaaaaatta cgacaaaggc ggctaattta gtagctgttg gtgatgaaat tgcggctgag
    61921 ttgggaattc ctatcgttaa taagcgtgta tcggtgacac ctatttctct gattggggca
    61981 gcgacagatg cgacggacta cgtggttctg gcaaaagcgc ttgataaggc tgcgaaagag
    62041 attggtgtgg actttattgg tggtttttct gccttagtac aaaaaggtta tcaaaaggga
    62101 gatgagattc tcatcaattc cattcctcgc gctttggctg agacggataa ggtctgctcg
    62161 tcagtcaata tcggctcaac caagtctggt attaatatga cggctgtggc agatatggga
    62221 cgaattatca aggaaacagc aaatctttca gatatgggag tggccaagtt ggttgtattc
    62281 gctaatgctg ttgaggacaa tccatttatg gcgggtgcct ttcatggtgt tggggaagca
    62341 gatgttatca tcaatgtcgg agtttctggt cctggtgttg tgaaacgtgc tttggaaaaa
    62401 gttcgtggac agagctttga tgtagtagcc gaaacagtta agaaaactgc ctttaaaatc
    62461 actcgtatcg gtcaattggt tggtcaaatg gccagtgaga gactgggtgt ggagtttggt
    62521 attgtggact tgagtttggc accaacccct gcggttggag actctgtggc acgtgtcctt
    62581 gaggaaatgg ggctagaaac agttggcacg catggaacga cggctgcctt ggccctcttg
    62641 aacgaccaag ttaaaaaggg tggagtgatg gcctgcaacc aagtcggtgg tttatctggt
    62701 gcctttatcc ctgtttctga ggatgaagga atgattgctg cagtgcaaaa tggctctctt
    62761 aatttagaaa aactagaagc tatgacggct atctgttctg ttggattgga tatgattgcc
    62821 atcccagaag atacgcctgc tgaaaccatt gcggctatga ttgcggatga agcagcaatc
    62881 ggtgttatca acatgaaaac aacagctgtt cgtatcattc ccaaaggaag agaaggcgat
    62941 atgattgagt ttggtggtct attaggaact gcacccgtta tgaaggttaa tggggcttca
    63001 tctgtcgact tcatctctcg cggtggacaa atcccagcac caattcatag ttttaaaaat
    63061 taagaaaata ggagaaattt taagttctat tt
//
LOCUS       DAVKGU010000003        58736 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKGU010000003 DAVKGU010000000
VERSION     DAVKGU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.003"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..222
                     /locus_tag="VW077_000156"
     CDS             <1..222
                     /locus_tag="VW077_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEU9454256.1"
                     /translation="NGSWYYLNANGDMETGWVKDGDTWYYLEASGAMKASQWFKVSDK
                     WYYVNGSGALAVNTTVDGYGVNANGEWVN"
     gene            complement(452..1432)
                     /gene="dusB"
                     /locus_tag="VW077_000157"
     CDS             complement(452..1432)
                     /gene="dusB"
                     /locus_tag="VW077_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9454257.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1419..2291)
                     /gene="hslO"
                     /locus_tag="VW077_000158"
     CDS             complement(1419..2291)
                     /gene="hslO"
                     /locus_tag="VW077_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9454258.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2456..3883
                     /locus_tag="VW077_000159"
     CDS             2456..3883
                     /locus_tag="VW077_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9454259.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4042..5550
                     /gene="glpK"
                     /locus_tag="VW077_000160"
     CDS             4042..5550
                     /gene="glpK"
                     /locus_tag="VW077_000160"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9454260.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKNQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5592..7418
                     /gene="glpO"
                     /locus_tag="VW077_000161"
     CDS             5592..7418
                     /gene="glpO"
                     /locus_tag="VW077_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9454261.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVKAALAQNDLAELKN"
     gene            7488..8192
                     /locus_tag="VW077_000162"
     CDS             7488..8192
                     /locus_tag="VW077_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9454262.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8489..8692
                     /locus_tag="VW077_000163"
     CDS             8489..8692
                     /locus_tag="VW077_000163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454263.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(8779..9366)
                     /locus_tag="VW077_000164"
     CDS             complement(8779..9366)
                     /locus_tag="VW077_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454264.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            10475..11752
                     /gene="ltrA"
                     /locus_tag="VW077_000165"
                     /pseudo
     CDS             10475..11752
                     /gene="ltrA"
                     /locus_tag="VW077_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            12204..12914
                     /locus_tag="VW077_000166"
     CDS             12204..12914
                     /locus_tag="VW077_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9454265.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLLYQQI
                     YWKFFQIRFGFLPLYLFL"
     gene            12860..13381
                     /locus_tag="VW077_000167"
     CDS             12860..13381
                     /locus_tag="VW077_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9454266.1"
                     /translation="MEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISG
                     LIAFRLSEKFLAAKWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVP
                     ESVFLQETVEENHLVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMI
                     EAILIYIRRDYFK"
     gene            13695..13826
                     /locus_tag="VW077_000168"
     CDS             13695..13826
                     /locus_tag="VW077_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9454267.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            13847..15397
                     /gene="dltA"
                     /locus_tag="VW077_000169"
     CDS             13847..15397
                     /gene="dltA"
                     /locus_tag="VW077_000169"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9454268.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIAQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            15394..16638
                     /gene="dltB"
                     /locus_tag="VW077_000170"
     CDS             15394..16638
                     /gene="dltB"
                     /locus_tag="VW077_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9454269.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            16652..16891
                     /gene="dltC"
                     /locus_tag="VW077_000171"
     CDS             16652..16891
                     /gene="dltC"
                     /locus_tag="VW077_000171"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9454270.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            16884..18152
                     /gene="dltD"
                     /locus_tag="VW077_000172"
     CDS             16884..18152
                     /gene="dltD"
                     /locus_tag="VW077_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9454271.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            18307..18747
                     /gene="adcR"
                     /locus_tag="VW077_000173"
     CDS             18307..18747
                     /gene="adcR"
                     /locus_tag="VW077_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9454272.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            18747..19451
                     /locus_tag="VW077_000174"
     CDS             18747..19451
                     /locus_tag="VW077_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9454273.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            19444..20250
                     /locus_tag="VW077_000175"
     CDS             19444..20250
                     /locus_tag="VW077_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9454274.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            20260..21765
                     /gene="adcA"
                     /locus_tag="VW077_000176"
     CDS             20260..21765
                     /gene="adcA"
                     /locus_tag="VW077_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9454275.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDVEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(22894..23667)
                     /locus_tag="VW077_000177"
     CDS             complement(22894..23667)
                     /locus_tag="VW077_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9454276.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPNQKLLDAVELFDGEWVSDIQ"
     gene            23843..25246
                     /locus_tag="VW077_000178"
     CDS             23843..25246
                     /locus_tag="VW077_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9454277.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFSTEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            25361..25999
                     /locus_tag="VW077_000179"
     CDS             25361..25999
                     /locus_tag="VW077_000179"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9454278.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            26011..26451
                     /locus_tag="VW077_000180"
     CDS             26011..26451
                     /locus_tag="VW077_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9454279.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            26444..26875
                     /locus_tag="VW077_000181"
     CDS             26444..26875
                     /locus_tag="VW077_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9454280.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            26866..27336
                     /locus_tag="VW077_000182"
     CDS             26866..27336
                     /locus_tag="VW077_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9454281.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            27361..28134
                     /locus_tag="VW077_000183"
     CDS             27361..28134
                     /locus_tag="VW077_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9454282.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            28131..28931
                     /locus_tag="VW077_000184"
     CDS             28131..28931
                     /locus_tag="VW077_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9454283.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            28990..31284
                     /locus_tag="VW077_000185"
     CDS             28990..31284
                     /locus_tag="VW077_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9454284.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELCIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            31294..34410
                     /locus_tag="VW077_000186"
     CDS             31294..34410
                     /locus_tag="VW077_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9454285.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            34467..36233
                     /locus_tag="VW077_000187"
     CDS             34467..36233
                     /locus_tag="VW077_000187"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9454286.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            36379..37530
                     /locus_tag="VW077_000188"
     CDS             36379..37530
                     /locus_tag="VW077_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9454287.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            37675..38559
                     /locus_tag="VW077_000189"
     CDS             37675..38559
                     /locus_tag="VW077_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9454288.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            38958..39209
                     /locus_tag="VW077_000190"
     CDS             38958..39209
                     /locus_tag="VW077_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9454289.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQATE
                     TSSALISSHYLDEQDLSEKLKSELQWLELENKLLNLWEH"
     gene            39197..39484
                     /locus_tag="VW077_000191"
                     /pseudo
     CDS             39197..39484
                     /locus_tag="VW077_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(39667..39794)
                     /locus_tag="VW077_000192"
                     /pseudo
     CDS             complement(39667..39794)
                     /locus_tag="VW077_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(39889..41220)
                     /locus_tag="VW077_000193"
     CDS             complement(39889..41220)
                     /locus_tag="VW077_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9454290.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPGKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(41242..42753)
                     /locus_tag="VW077_000194"
     CDS             complement(41242..42753)
                     /locus_tag="VW077_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9454291.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            complement(42962..43909)
                     /gene="arcC"
                     /locus_tag="VW077_000195"
     CDS             complement(42962..43909)
                     /gene="arcC"
                     /locus_tag="VW077_000195"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9454292.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            complement(44074..45090)
                     /gene="argF"
                     /locus_tag="VW077_000196"
     CDS             complement(44074..45090)
                     /gene="argF"
                     /locus_tag="VW077_000196"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9454293.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            complement(45148..46377)
                     /gene="arcA"
                     /locus_tag="VW077_000197"
     CDS             complement(45148..46377)
                     /gene="arcA"
                     /locus_tag="VW077_000197"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9454294.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDESKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            47132..48811
                     /locus_tag="VW077_000198"
     CDS             47132..48811
                     /locus_tag="VW077_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9454295.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGSLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIYVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            complement(48851..50935)
                     /locus_tag="VW077_000199"
     CDS             complement(48851..50935)
                     /locus_tag="VW077_000199"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9454296.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGKAIHMDLATGTVNPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLNKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            51106..52386
                     /locus_tag="VW077_000200"
     CDS             51106..52386
                     /locus_tag="VW077_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9454297.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDSLLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            52477..55122
                     /locus_tag="VW077_000201"
     CDS             52477..55122
                     /locus_tag="VW077_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9454298.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFELFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVQLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTSFTSLQLARQEGS
                     VVAIGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            55205..56074
                     /locus_tag="VW077_000202"
     CDS             55205..56074
                     /locus_tag="VW077_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9454299.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSIPTPKNLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHCFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAGKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNCNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            56068..57948
                     /locus_tag="VW077_000203"
     CDS             56068..57948
                     /locus_tag="VW077_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9454300.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            58326..58688
                     /locus_tag="VW077_000204"
     CDS             58326..58688
                     /locus_tag="VW077_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454301.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
BASE COUNT        17747 a        10751 c        12936 g        17302 t
ORIGIN      
        1 aatggctcat ggtactacct aaacgctaat ggtgatatgg agacaggttg ggtgaaagat
       61 ggagatacct ggtactatct tgaagcatca ggtgctatga aagcaagcca atggttcaaa
      121 gtatcagata aatggtacta tgtcaatggc tcaggtgccc ttgcagtcaa cacaactgta
      181 gatggctatg gagtcaatgc caatggtgaa tgggtaaact aaacctaata taactagtta
      241 atactgactt cctgtaagaa ctctttaaag tattccctac aaataccata tcctttcagt
      301 agataatata cccttgtagg aagtttagat taaaaaataa ctctgtaatc tctagccgga
      361 tttatagcgc tagagactac ggagtttttt tgatgaggaa agaatggcgg cattcaagag
      421 gctctttaag agagttacgg gttttaaact attaagcctt ctccaattgc aagagggctt
      481 caatctctgc cagggtgcta gcttgcgaaa tggctccacg gagtttggca gcgccagatg
      541 ttccacggag gtagtgagga gcgaggccgc ggaattcacg aactgcgacg ttttctcctt
      601 tgaggttaat caatcgtttc aagtgttcgt aggcgatctt catcttgtct tcaaaggtca
      661 aatcaggtag gatttctcct gtttcaaagt aatggttgat ttggttgaag aggtaaggat
      721 ttcccatggc agctcggcca atcatgactg cgtcagcacc aacttcttcg atgcgttgct
      781 tggcttcttg gacagtacgg atatcaccgt tggcgatgaa tggaatcttg gttagagctt
      841 gggcaacctt gtaaagggtc tcaaggtctg cgtggccagt atacatttgt tcacgggtac
      901 ggccatgcat ggcgagggca gaaacacctg cagcctcagc agcgagggca ttctctactg
      961 ccagagatgg gtccgcccag ccggtacgca ttttgacagt aagtgggata tcaaggacag
     1021 actggacctt gttgatgata gagtagatct tgtcaggatc cttgagccac atagcaccag
     1081 cttcgttctt cacgattttg ttgacagggc agcccatgtt gatatcgacg atatcggtct
     1141 tagtgttttc ttggataaat tctgctgcgc gtgctaggct gtcttcatcg ctaccaaaaa
     1201 gttggataga gacagggttt tcgccctcat cgatatgaag catgtgcagg gttttttcgt
     1261 tgttgtattg gattcccttg tcagagacca tttccattac aacgagtcca gctccgagct
     1321 cttttgcaat ggtacgaaag gctgagttgg tcacgccagc cataggcgct aaaacggtac
     1381 gattgggaat ctcaacattg ccaatcataa aaggtgtatt aagatttgtc acgaatgagt
     1441 tcctccaggt ccttttcatc aaagttgtaa gtagtttggc agaattgaca agtgatttct
     1501 gccccgtggt cttcctcttt catttcctgt aagtctgagc ttggaaggct ggcaagagcg
     1561 ttcataaagc gttcatggct acagtcacat tggaaacgga tttcttcttc agaaagacgc
     1621 ttgtaggcct cgtcaccgta gatagccttg aggagggctt cgatatggtc gtcgctttcg
     1681 agaagagtag agatagctgg catttcttgg atgcgttttt caaagcgagc aatctcttct
     1741 ttcttggctc ctggcaagac ttgaactagg aaaccacctg caaccttgac cttgtcttcc
     1801 tcgtccaaaa ggacattgag gccgaccgct gaaggcgttt gttggctttc agtaaggtaa
     1861 aaggcaaggt cttcaccgat ttctccagag atgaggggag ttatagagtt gtaaggattt
     1921 ccagtaccgt agtctgtgat aacaaggaat tgaccatttc caacaaaagg tccgactagg
     1981 acttcaccag tcgcagtctt tttgatgtca acaccaggat tttgaacata gcctttgacg
     2041 ttccccttgg tatcagcgac ggtgataata gcacctagag agctagagcc caacacctta
     2101 actgtaagtt tggtatttcc tttttcattg gctgcgagaa tctggctggc gataagagtt
     2161 cgaccaagcg ctacagttga gctagcttgg gtttgatgtt tttcttgagc agtgcggacg
     2221 gtttcagtgc tatcaaggac aaaagcacga aaggctccgc tttctgatat agttttaata
     2281 attttatcca tagctactat tttagcataa aaatgcccaa agggggagcc gtgtgtttac
     2341 tgattttcag gataatggac caggaaatca gcatgaaaat aaaaagagaa acagattatt
     2401 ttagcatttg taagatttat gctatgctta aggtagaaaa tgaaaggggt aacaaatgta
     2461 tttaggagat ttgatggaga aagccgagtg tggtcaattt tcaatccttt cctttctatt
     2521 acaagagtct cagacgaccg tcaaggctgt aatggaagaa acaggatttt caaaagcaac
     2581 cctaaccaaa tatgtcaccc tgctcaatga caaggctttg gatagtggct tagagctgac
     2641 tattcactca gaagatgaaa atctgcgtct gtctatcggt gcagctacca aggggagaga
     2701 tattcggagc ttgtttttgg agagtgctgt taaataccag attttggttt atcttctcta
     2761 ccaccaacag tttttagccc atcagctggc tcaagaatta gtgattagcg aggctacgct
     2821 tggtcgtcac ttggctggtt taaatcagat tttgtcagaa tttgatttat ccatccaaaa
     2881 tggacgttgg cgaggtccag agcatcagat tcgctatttc tatttctgtc ttttccgaaa
     2941 ggtctggtcg agtcaggaat gggaaggtca catgcagaaa ccagagagaa aacaggagat
     3001 tgccaattta gaggaaatct gcggtgcaag tttgtctgcg ggacagaaat tggacttggt
     3061 tctctgggct cacatcagtc aacaacgtct tcgggtcaat gcttgtcagt ttcaagtcat
     3121 agaagagaaa atgcgagggt attttgacaa tatcttctat cttcgtttgc tgagaaaggt
     3181 tccgtccttt tttgctgggc aacatattcc actaggagtt gaggatggtg agatgatgat
     3241 attcttctct tttctcctat ctcatcgcat tcttcctctt catactatgg aatatattct
     3301 tggttttgga gggcagttgg cagatttact gacgcaattg attcaagaaa tgaagaagga
     3361 ggaactattg ggggattata cagaggacca tgtcacctat gaactcagtc agctttgtgc
     3421 tcaagtctat ctctataagg gctatatttt acaggatcgc tacaagtacc agttagagaa
     3481 tcgtcatcca tatttactga tggaacatga ttttaaagag acagcagagg agatttttca
     3541 tgctctacct gcttttcaac aggggacaga tttagataag aagattctct gggaatggct
     3601 ccagttaatc gaatatatgg ctgaaaacgg tggccagcat atgcggattg gtctggattt
     3661 gacatctggt tttcttgtct tttcaaggat ggcagccatt ttgaaacggt atttggaata
     3721 caatcgtttt attaccattg aagcttatga ccctagtcgg cattatgatt tgctggttac
     3781 caataacccg attcataaga aggaacagac accagtctat tatttaaaaa atgacttgga
     3841 tatggaggat ttggcaggga ttcgtcagtt attattcact taaaaggctt ggttaatcca
     3901 ggtctttttt gtgaaattca cacaatctcc tcacattttt ttaaaaatta aaaaaagttg
     3961 ataaacaaga aagcgcttta ttttgtatac tagtaagtgt aaagaggaaa cacctcaaga
     4021 tctttatcag gaggacagta tatgtcacaa gaaaaataca tcatggccat tgaccaggga
     4081 actacaagtt ctcgtgccat cattttcaac aaaaaagggg aaaaggttag ctcgagtcaa
     4141 aaagagttta cccagatttt ccctcaggca ggttgggttg agcacaatgc caatgaaatt
     4201 tggaactctg ttcagtcagt tattgcgggt gctttcatcg aaagtggtgt caagccaaat
     4261 caaatcgagg caatcgggat taccaaccaa cgtgaaacaa cggttgtctg ggataagaaa
     4321 acaggacttc ctatctacaa tgctatcgtt tggcagtcac gccagacagc acctttggct
     4381 gagcaactaa aaaaccaagg ttatgtggaa aaattccatg aaaagactgg tttgattatt
     4441 gatgcttact tctctgctac caaggttcgt tggattttgg atcatgtaga aggcgctcaa
     4501 gagcgagcag aaaaagggga attgctcttt ggtactatcg atacttggtt ggtttggaaa
     4561 ttgactgacg gtgcggctca cgtgactgac tactcaaatg cagctcgtac catgctttat
     4621 aacattaaag aactcaaatg ggatgatgaa attttggaaa tccttaacat tccgaaggct
     4681 atacttccag aagttcgttc taactccgaa atctacggca agacagctcc attccatttc
     4741 tacggtggag aggtgccaat ctcaggtatg gctggggacc aacaagcagc cctctttgga
     4801 cagttggctt ttgagccagg tatggttaag aatacttatg gaacaggctc tttcatcatc
     4861 atgaatactg gggaagagat gcagttgtct gaaaacaacc tcttgacaac cattggttac
     4921 ggaatcaacg gtaaggttta ttatgccttg gaaggttcta tcttcatcgc aggaagtgct
     4981 attcagtggc ttcgtgacgg tcttcgcatg gttgaaaatt caccagaatc tgaaaaatac
     5041 gctcgtgatt ctcacaacaa cgatgaagtt tatgtcgttc cagcctttac aggtctaggc
     5101 gctccatact ggaaccaaaa tgctcgtggt tccgtctttg gtttgactcg tggaacaagc
     5161 aaagaagact ttatcaaggc gactttgcaa tctattgctt atcaagtgcg tgacatcatc
     5221 gacaccatgc aagtggatac tcagaccgcc attcaagtac tgaaggtgga tggtggtgca
     5281 gccatgaaca acttcctcat gcaattccag gcggatattt taggcattga cattgcacgt
     5341 gctaaaaact tggaaacaac agctctagga gcggccttcc tagcaggttt gtcagtaggg
     5401 tactggaaag acttggacga gttgaaactc ttgaacgaga caggagaact ctttgagcca
     5461 tctatgaatg aatctcgcaa ggaacaactc tacaagggct ggaagaaggc tgtgaaagca
     5521 actcaagtct ttgcggaagt agacgactaa tactggcaga ataaagcgat ttatttagaa
     5581 agtgtgtaaa tatggaattt tcaaagaaaa cacgtgaatt gtcaattaaa aaaatgcagg
     5641 aacgcaccct ggacctcttg attatcggtg gaggaatcac aggagctggt gtagccttgc
     5701 aggcggcagc tagcggtctt gagactggtt tgattgaaat gcaagacttt gcagaaggaa
     5761 catctagtcg ttcaacaaaa ttggttcacg gaggacttcg ttacctcaaa caattcgacg
     5821 tcgaagtggt ctcagatacg gtttctgaac gtgcagtggt tcaacaaatc gctccacaca
     5881 ttccaaaacc agacccaatg ctcttaccag tttacgatga agatggagca acctttagcc
     5941 tcttccgtct taaagtagcc atggacttgt acgacctctt ggcaggtgtt agcaacacac
     6001 caactgcgaa caaggttttg agcaaggatc aagtcttgga acgccagcca aacttgaaga
     6061 aggaaggctt ggtaggaggt ggagtgtatc ttgacttccg taacaacgat gcgcgtctcg
     6121 tgattgaaaa catcaaacgt gccaaccaag acggtgccct cattgccaac cacgtgaagg
     6181 cagaaggctt cctttttgac gaaagtggca agattacagg tgttgtagct cgtgatctct
     6241 tgacagacca agtatttgaa atcaaggccc gtctggttat taatacaaca ggtccttgga
     6301 gtgataaagt acgtaatttg tctaataagg gaacgcaatt ctcacaaatg cgcccaacta
     6361 agggagttca cttggtagtg gattcaagca aaatcaaggt ttcacagcca gtttactttg
     6421 acacaggttt gggtgacggt cgtatggtct ttgttctccc acgtgaaaac aagacttact
     6481 ttggtacaac tgatacagac tacacaggtg atttggagca tccaaaagta actcaagaag
     6541 atgtagatta tctacttggc attgtcaaca accgctttcc agaatccaac atcaccattg
     6601 atgatatcga aagcagctgg gcaggtcttc gtccattgat tgcagggaac agtgcctctg
     6661 actataatgg tggaaataac ggtaccatca gtgatgaaag ctttgacaac ttgattgcga
     6721 ctgttgaatc ttatctctcc aaagaaaaaa cacgtgaaga tgttgagtct gctgtcagca
     6781 agcttgaaag tagcacatct gagaaatatt tggatccatc tgcagtttct cgtgggtcta
     6841 gcttggaccg tgatgacaat ggtcttttga ctcttgctgg cggtaaaatc acagactacc
     6901 gtaagatggc tgaaggagct atggagcgcg tggttgacat cctcaaagca gaatttgacc
     6961 gtagctttaa attgatcaat tctaaaactt accctgtttc aggtggagaa ttgaacccag
     7021 caaatgtgga ttcagaaatc gaagcctttg cgcaacttgg agtatcacgt ggtttggata
     7081 gcaaggaagc tcactatctg gcaaatcttt acggttcaaa tgcaccgaag gtctttgcac
     7141 ttgctcacag cttggaacaa gcgccaggac tcagcttggc agatactttg tcccttcact
     7201 atgcaatgcg caacgagttg gctcttagcc cagttgactt ccttcttcgt cgtaccaacc
     7261 atatgctctt tatgcgtgat agcttggata gcatcgttga gccagttttg gatgaaatgg
     7321 gacgattcta tgactggaca gaagaagaaa aagcaactta ccgtgctgat gtaaaagcag
     7381 ctcttgctca aaacgactta gcagaattaa aaaattaaga aaaaataaaa gaggtggagg
     7441 gcagcattcc ttgtcgcccg tcccttcttt ttaatggaga cagaaagatg atgaatgaat
     7501 tatttggaga atttctaggg actttaatcc tgattcttct aggaaatggt gttgttgcag
     7561 gtgtggttct tcctaaaacc aagagcaata gctcaggttg gattgtgatt actatgggtt
     7621 gggggattgc agttgcggtt gcagtctttg tatctggcaa gctcagtcca gctcatttaa
     7681 acccagctgt gaccatcggt gtggccttaa aaggtggttt gccttgggct tccgttttgc
     7741 cttatatctt agcccagttc gcaggggcca tgctgggtca gattttggtt tggttgcaat
     7801 tcaaaccgca ttatgaggta gaagaaaatg caggcaatat cctggcaacc ttcagtactg
     7861 gaccagccat caaggatact gtatcaaact tgattagcga aatccttgga acctttgttt
     7921 tggtgttgac aatctttgct ttgggtcttt acgattttca ggcaggtatc ggaacctttg
     7981 cagtgggaac tttgattgtc ggtatcggtc tatcactagg tgggacaaca ggttatgcct
     8041 tgaacccagc tcgtgacctt ggacctcgta tcatgcacag catcttgcca attccaaaca
     8101 agggagacgg agactggtct tacgcttgga ttcctgttgt aggccctgtt atcggagcag
     8161 ccttggcagt gcttgtattc tcacttttct agtttatact cttcgaaaat caaattcaaa
     8221 ccacgtcagc gtcgccttac cgtactcaag tacagcttgc ggctagcttc ctagtttgct
     8281 ctttgatttt cattgagtat tagaaaacaa ttattttgat agagcttggg caagagccca
     8341 atttcagcaa aaaatgaagt aaatcttttc ataataaaac gcatcatatc aagcacgaaa
     8401 attccacgag gtcaactaca gtcagaaagc tgaacaacaa gccaaaacgc ccaaaaaagg
     8461 cggcaaaaag caagcacctg caagcaacgt gccgaaatgg tcaaatcctg attatgtcaa
     8521 cgaattagac ccaaaaatcg ttgatatgct agtagaattt cacaagtcac aaggcacttt
     8581 ggaaactccc gaggcgcaag cagaaatcgc ccaaaaacgt gaagaaatcg agcaaaggag
     8641 agctgagctt gagggtaaaa aacaagagct tttgaaccgc ttgaacaaat agagtttcgc
     8701 aagtattatg cttacaaatt acttgagcaa ttaactaaaa tataaaccct gcctttatat
     8761 ctaggcaggg tttatatttt agaaattcac gtaggttgtt acggttttta catacccagt
     8821 atagtttgag tttctatagt attcagtgat aaacttccat tttctttgag caacatggat
     8881 ataagtactt gttatgtagt atggatatgg gctttgtgaa tccaagtaag actgataagc
     8941 ttgtatacca aaatatgctc caccaattat tgcaccccat ggacccccca ataaagcacc
     9001 tatcctacca atcatataac tgattccagc accagtcatg aagttagcga atgtgttagc
     9061 ttgtttattc ccatgtattg tgttgacgta attccaaaca ttaggatcgt atgatctaaa
     9121 agatatattt aggtcgattt cattcttttg ataagccata taaaatgccc cattgatata
     9181 gacgccgtca gcacgtcgtt caatagtgtc tacacttcca tctggattga caacctcaag
     9241 aacttcatcg cttaaaatat ttacttgcgt atctccgaac cgcactgatg agccattctc
     9301 aaactgagcc tcaccagata caactttaga gtttgccgat aagctatcat cagcaaaaac
     9361 aaacaagcga cggggaaatg ctagacatac agaaaacaga cataactagc aaacacatgc
     9421 atttaaacat cttagacata acggaaactc ctttgtattt ttgatttttt tcaactttta
     9481 ttatacaata aaaccaaata aaaagaaagc ggtaacaata tgcttaatgc gaaaattttt
     9541 tatatatttt tatgtttgat cgttatcgaa actacaggct tgttgttgtt gaaaagaggt
     9601 ctcgaaatgg gttatttaga cacagaagct attatcctcg cagttttttc atttgctttt
     9661 tacaacctat gttcattcgc ttgggtctgc tctacaataa aaaacaataa aaaataaata
     9721 gacgtatttt caaaaaaaaa aatgcatatt tatcttatcg aaacgacgat ttaaatcgtc
     9781 gtttttttgt agtacgacgg gcatgtcgta tatctgaggt gtaagtcctc ggtgggcacc
     9841 tgctaccggt gaacccaata gcgattccca agcctgacta tcgtgaggta gcagggagag
     9901 gaagggatag cgaaatcgtg gctctacgaa caggaacgtg atagtaaggc gtatatagcg
     9961 gataaggagg cttcaaactc taaagtccaa aaaggtagtc gtaacctata tgtgtaaatc
    10021 acgagagtaa ttgaattcgg actaaggttt gtgtgaaaaa gataaatctt tctagagtct
    10081 aaagactctg cgtcagattt cctattttca ctgtaacctt ttaacgtcct catatcttgt
    10141 atacacgagg aaagatgtac gacttatccc gtgaggtttc atgagcgctg aaagcgtagt
    10201 aacaacgaat catgagaagt cagccgagcc catagtagtg aggaaacttc tgtagtggaa
    10261 gtggagcgaa ggggtgaata ctcaaacagt ctggggagag actgtttgag gtctgtcgct
    10321 agaaagagaa aacgacagat cgaagtaatc ctacttcact tgtgtctgta aaatgagtgg
    10381 tctgatagaa ctggactttg acacgtaatg actagatgaa ggttcaccaa tataagatgt
    10441 ccctcaggca ccaaaacaag aaaggaatac gcacatgtca aaactgctag ataagatatt
    10501 atcacgcgaa aatatgctgg aagcctacaa tcaagtaaaa tccaataaag gctcagctgg
    10561 gattgatgga atgactatcg aagagatgga taattatctc agacaaaact ggcgcttgac
    10621 taaggaactg ataaaacaga gaaaatataa gcctcaacca gttcttagag ttgagatacc
    10681 taaaccagac ggaggtatcc gtcaactagg aattccaaca gttatggata gaatgattca
    10741 acaggccatt gtctaagtca tgagccccat ttgtgaaccc catttctcag atacgagtta
    10801 tggtttcaga ccaaataggt catgtgaaaa agccatcatg aagctcttag aatacttaaa
    10861 tgacggctat gagtggatag tggatataga cctagagaaa tttttcgata cagttcctca
    10921 agatagattg atgtccttag tacataacat tatcgaagac ggagatacgg aatccttgat
    10981 tcgtaagtat cttcattcag gtgttatcat taatggtcaa cgttataaaa cactagttgg
    11041 tacaccacag ggaggaaatt tatctcctct cttatccaat atcatgctta atgaattgga
    11101 caaggaatta gaaaagaggg gacttcgatt tgtgcgctac gcagatgatt gtgtgattac
    11161 ggtcgtaagc gaggcagccg ctaagcgtat gatgtattca gtcagtcgtt ttattgaaaa
    11221 acggctaggt ttgaaagtaa acatgaccaa gactaagatt accagaccaa gagagttgaa
    11281 atatctaggt tttgggttct ggaaatcatc agatggttgg aaaagtcgtc cacatcaaga
    11341 tagtgttcgg agatttaagc ttaaattgaa gaaactaaca cagaggaaat ggagtataga
    11401 cctaacaaga cgtattgagc aactgaattt gtctattcga ggatggataa actatttctc
    11461 attgggaaat atgaaaagta tagtcgccag catagatgag cgcttgcgta ctcgcctacg
    11521 agtgattatc tggaagcaat ggaagaagaa atcgagacga ttatggggat tgcttaagtt
    11581 aggggttcct aaatggatag cagataaggt atctggctgg ggcgaccatt atcaattagt
    11641 agctcagaag tcggtactta aacgtgctat atcaaaacca gtcctggaaa aacgtggact
    11701 ggtttcgtgt ttggattatt accttgaacg acatgcgtta aaagttagtt gaaccgctgt
    11761 atgccgaacg gcacgtacgg tggtgtgaga ggggctagag attatcccct actcgattaa
    11821 ctcccctgaa atttatttta attatgcaaa tttcacgtat ttttgatgct gagacgacga
    11881 tcctgggaac ttttcagata tttttttgac tatctaaatc tatcattaga aaagcttaga
    11941 gcgccaaagg atttgagcgt ttttctgatt tttaagactt tttccagtct ctttttcgat
    12001 tgaagatgta attattctac taactaactt cttagtacta gccaacaacg ataatcataa
    12061 ttcctcctaa aattaggaat aataaaggca atagtttttg ttttttcatg taaaaaacct
    12121 cacttttgtt ttctgctatt ttatgctaaa atattaaaaa tcaaatttaa ttccaaagtt
    12181 tgtaactaaa gggggagcgc tacatgtcta attcatttgt caagttgtta gtctctcaat
    12241 tatttgcaaa tttagcagat attttcttta gagtaacaat cattgctaac atatacatta
    12301 tttcaaaatc agtaattgcc acatcactag ttcctatctt aataggaata tcctcttttg
    12361 ttgcgagtct tttagttccg ttggttacta aaaggttagc gctaaatagg gttttatctt
    12421 tatctcaatt tggaaagact atattattgg cgatactggt aggaatgttt accgtaatgc
    12481 aatccgtagc gcctttggtg acctatctat ttgttgttgc aatttccata ctagatggtt
    12541 ttgcagcacc cgtttcctat gctattgtgc cacgctatgc gaccgatttg ggtaaggcta
    12601 attcagcctt atcaatgact ggtgaagctg ttcaattgat aggttgggga ttaggtggac
    12661 tcttgtttgc aacaattggt ctgttaccta ccacgtttat cattttagtc ttgtatatca
    12721 tttctagctt tctgatgtta tttcttccta acgctgaagt ggaggtgtta gagtcagaaa
    12781 ctaatcttga aattttgctc aaaggttgga agttagttgc tagaaatcct agattaagac
    12841 ttttgtatca gcaaatttat tggaaatttt ttcaaatacg atttgggttt cttccattat
    12901 acttgttttt gtaacggagt tattaaataa aacggaaagt tactggggat attctaatac
    12961 agcatactct attggtatta taattagtgg cttaattgct tttaggctat ctgaaaagtt
    13021 ccttgctgct aaatgggaaa gtattctttt tcctcttgta gctatggcaa tagtgacact
    13081 gactattcta tattttccaa acgcacagat gtttttatta ttttcagctt tagttggtat
    13141 gttatcgcaa ctaaaagaag ttcctgaaag tgtatttctt caggaaacag tagaggaaaa
    13201 tcatttagtt aatgtctatt ccgttcttga agtgatttct acattagcat tttcagtatt
    13261 tgttttgcta atgagctata ttactgagag ttttggtatt agcatcagtt tttggctatc
    13321 agccatttgt ctgatgatag aagctatttt aatttatatc agacgagatt attttaaatg
    13381 agagagtggt taaaaagtct ttaaaaatca gaaaagcgca tagtatcagg tgttgaaaaa
    13441 acttgatatt gtgtgtttta ttatgagaag atttacttca ttgtctcctg aaatggagtt
    13501 ttgaagcagg atcactctat ttgaaaatat acatagaaaa actgtataat ccttaaaata
    13561 agctgaaatc tattggaaac atacggtaaa caattgaaaa gatactatat aaagtggtac
    13621 aatggtagaa aaataagcta gtaagaatca ctcttatttt agcgaaaaat tacagaaatg
    13681 aatggttttt cttgatgaaa cagagaaaag aattgtacct ctttcttggt cggacagcct
    13741 tgtattttct tatctttcta gggctgcttt acttctttag ctatcttggt cagggtcaag
    13801 gaagctttat ctataatgaa ttttaacttg aaggagaatc cctattgtgt caaataaacc
    13861 aatagcagat atgattgaaa ccattgagca ttttgctcag acacagccta gctatcctgt
    13921 ctataatgtt ttggggcagg aacacactta tggcgattta aaggctgatt cggatagttt
    13981 ggctgcagtc attgcccaac taggcttgcc tgagaagtct cctgtggttg tttttggtgg
    14041 ccaagaatat gaaatgttgg caacctttgt agcgctgact aagtcaggtc atgcctacat
    14101 tccaattgat agccattcgg ccttggagcg agtttcagct attttagaag tagcagagcc
    14161 aagcttgatt attgccattt cagcctttcc cttggagcag gtttctacac caatgataaa
    14221 tctagctcag gttcaagaag cctttgccca agggaacaac tatgaaatca cgcatccagt
    14281 caagggagat gataattact acattatctt tacttctggt acgactggta agcctaaggg
    14341 agtgcagatt tcacatgata atctcctcag ctttacaaac tggatgatta cggataagga
    14401 atttgcgaca ccgagtcgtc cgcaaatgct ggcacagcca ccttattctt ttgacttgtc
    14461 tgtcatgtat tgggcaccga ccttggcact tggtggtacg cttttcactc ttccttcagt
    14521 catcactcag gactttaagc aactctttgc ggctatcttt tcattgccaa tcgctatctg
    14581 gacatcaaca ccatcctttg cagatatggc catgttgtct gaatacttca acagtgagaa
    14641 aatgcctgga atcacgcatt tctactttga tggtgaagaa ttgacggtca aaacagctca
    14701 aaaactgcgc gagcgtttcc caaatgcccg tatcatcaat gcttacggcc caacagaagc
    14761 gacagtagct ctgtcagcag ttgccgtgac agacgagatg ttagcgactc tcaaacgcct
    14821 accaatcggc tataccaagg ctgattctcc aacctttatc attgacgagg aaggaaataa
    14881 actgccaaat ggtgagcagg gagaaatcat tgtttctggg ccagctgttt caaaaggtta
    14941 tatgaaaaat cctgaaaaaa cagcagaagc cttctttgag tttgaaggtc tgccagccta
    15001 tcacacaggc gatgtgggaa ccatgacaga tgagggcttg cttctctacg gcggacgcat
    15061 ggacttccag attaagttta acggttaccg cattgagtta gaagatgtct ctcaaaacct
    15121 caacaagtct cgctttatcg aatctgctgt cgcagtaccg cgctataaca aggaccacaa
    15181 ggtacaaaat ctattggctt atgtcatctt aaaagacggt gttcgtgagc agtttgagcg
    15241 agatatcgat attaccaagg ccatcaagga agacctgaca gacatcatga tgtcctatat
    15301 gatgccatct aaattccttt accgagacag tttgccacta actccaaatg gaaagattga
    15361 catcaaagga ttgattaacg aggtgaataa gagatgatgg agttttttca acagcttcct
    15421 catttagagc catatggcaa tcctcagtat tttgtttatg tgattgctgc aaccttgccc
    15481 atctttatag gtctcttttt caagaaacgc tttgcctggt atgaagtgtt ggtaagtctc
    15541 ttctttattg tcaccatgtt ggtgggtgga aagaccaatc aactagctgc cttgggtatt
    15601 tacctttgct gggaaatatt gctcctgctt ttctacaagc attatcgaaa aagcaaggat
    15661 ggcaagtggg tcttctactt agttagtttt ctgtccctac ttccgattat ctttgtcaag
    15721 gtgcaaccag ctatcaatgg aacgcagtct ttgcttgggt tcttgggaat ttcttacctg
    15781 acctttcgtt cggttgggat tatcatcgag ctgagagatg gagtgattaa ggattttacc
    15841 ctctgggaat tcctccgttt ccttctcttc atgccaactt tctcgagtgg tccaatcgat
    15901 cgctttaagc gatttaatga aaattatcag gccattcctg agcgagatga gttgatggat
    15961 atgctggatg aatctgtccg ctatatcatg cggggctttt tgtataagtt tatcctagct
    16021 catgttttag gagagacctt actacctcct ctgaagaatt tagccttgca gtcaggtggc
    16081 ttctttaatc tctatgcctt ggcagttatg tatacttttg gtctggaact cttctttgac
    16141 tttgcaggtt attctatgtt tgctttggcc atctcaaact tgatgggaat ccgtagccct
    16201 atcaacttta acaagccctt tttatcaagg gatttaaagg agttttggaa tcgctggcat
    16261 atgagtctgt ccttctggtt ccgtgacttt gtctttatgc gaatggtgat ggtgttaacc
    16321 agaaagaaag tctttaaaaa tcgtaatgta acctcaagca tggcctacat tgtaaatatg
    16381 ctgattatgg gattttggca tggtgtgacc tggtactata tcgcctatgg actctttcat
    16441 ggactaggct tggtcatcaa tgatgcctgg gttcgcaaga aaaaaacgct caataaggaa
    16501 cggaaaaaag cagggaaggc tgccctacct gagaatcgct ggattcagtt gcttggcatg
    16561 gttgtcactt tccatgttgt catgttgtca ttcttaatct tttctggatt cttgaataat
    16621 ctatggttta aaaaataaaa ggaaataaaa tatggatatc aaatcagaag ttattgaaat
    16681 tattgatgag ttgtttatgg aagatgtttc tgacatgatg gatgaagatc tttttgatgc
    16741 aggtgtcttg gatagtatgg gaacggttga gttgattgtg gagattgaga accgttttga
    16801 cattcgtgtc cctgtaacag agtttggtcg tgacgactgg aatacagcta ataaaatcat
    16861 agctggtatt gtggagctac aaaatgctta aacgcttatg gatgatcttc ggaccggtct
    16921 tgatcgctgg tttgttggtt tttctgctca ttttctttta tcctactgag atgcatcata
    16981 atctaggagc tgaaaagcgt tcagcagtgg ctactactat cgatagtttt aaggagcgaa
    17041 gtcaaaaagt cagagcacta tctgatccaa atgtgcgttt tgttcccttc tttggctcta
    17101 gtgaatggct tcgttttgac ggtgctcatc ctgcggtatt agctgagaaa tacaatcgtt
    17161 cctaccgtcc ttatctttta ggacaggggg gagctgcatc gcttaaccaa tattttggaa
    17221 tgcaacagat gttaccacag ctggagaata aacaagttgt gtatgttatc tcacctcagt
    17281 ggttcagtaa aaatggctat gatccagcag ccttccagca gtattttaat ggagaccagt
    17341 tgactagttt tctgaaacat caatctgggg atcaggctag tcaatatgca gcgactcgct
    17401 tactgcaaca gttcccaaac gtagctatga aggacctggt tcagaagttg gcaagtaaag
    17461 aagaattgtc gacagcagac aatgaaatga ttgaattatt ggctcgtttt aatgaacgcc
    17521 aagcttcctt ttttggtcag ttttcggtta gaggctatgt taactacgat aagcatgtag
    17581 ctaagtattt aaaaatcttg ccagaccagt tttcttatca ggcaatagaa gatgttgtca
    17641 aagcagatgc tgaaaaaaat acttccaata atgagatggg aatggaaaat tatttctata
    17701 atgagcagat caagaaggat ttgaagaaat taaaggattc tcagaaaagc tttacctatc
    17761 tcaagtcgcc agagtataat gacttgcagt tggttttaac acagttttct aaatctaagg
    17821 taaacccgat ttttatcatt ccacctgtta ataaaaaatg gatggactat gctggtctac
    17881 gagaggatat gtaccaacaa acggtgcaga agattcgcta ccagttagaa agtcaaggtt
    17941 ttaccaatat agcagatttt tctaaggacg gcggggagcc tttctttatg aaggacacca
    18001 ttcaccttgg ttggttgggt tggttggctt ttgacaaggc agttgatcct ttcctatcca
    18061 atcccacacc agctccgact taccatctga atgagcgctt tttcagcaaa gattgggcga
    18121 cttatgatgg agatgtcaaa gaatttcaat agataatata atatagaaga gttcaagaat
    18181 gaaactagtt ttcacgtttt ttcttgactc tttttttgct tgtgatataa ttaactggta
    18241 aacaaaatgt caaagaatag tatttttctc ttaaaaaaga gaagtctaaa aggaaaggag
    18301 tcagatatga gacagctagc aaaggatatc aatgcttttt tgaatgaggt gattttgcag
    18361 gcggaaaatc agcatgaaat cctaataggt cattgcacta gcgaggtggc cctgaccaat
    18421 actcaggagc atatccttat gctcttgtca gaggaatctt taacaaattc agaattggct
    18481 cgtcgtctca atgtcagtca ggcggcagtt accaaggcca ttaagtcttt ggtcaaggaa
    18541 gggatgttgg aaacatctaa agattctaaa gatgcgcgtg tgatttttta tcagttgact
    18601 gacttggctc gtccaatcgc tgaggagcac caccatcacc atgagcatac acttttaacc
    18661 tatgaacaag tggcgactca gtttactcca aatgaacaaa aagtgattca gcggtttttg
    18721 actgctttag taggagaaat caaataatga gatatattac ggtagaggat ttgtccttct
    18781 attatgataa ggagcctgtt cttgaacata tcaattatag tgttgatagt ggggaatttg
    18841 ttaccttgac tggggaaaat ggagcggcta agacgacgct catcaaggct agtcttggaa
    18901 ttctgcaacc acgcattgga aaggtggcta tttcaaagac aaatacgcaa ggtaagaaat
    18961 tgagaatagc ctatcttcct caacaaattg ccagttttaa tgctggtttt ccaagtacgg
    19021 tctatgaatt tgtcaagtcg ggtcgctatc cgagaaaagg ctggttccgt cgtttgaatg
    19081 ctcatgatga ggagcatatc aaggctagtc tggactcagt tggcatgtgg gaacatcgag
    19141 acaaacgctt ggggtctcta tctgggggac aaaagcagcg agcggtaatt gcgcgtatgt
    19201 ttgcttctga ccctgatgtg tttatcctag acgagccgac aacggggatg gatgcaggaa
    19261 gtaaaaatga attttacgaa ctcatgcacc acagcgccca tcatcatggc aaggctgttt
    19321 tgatgattac ccatgaccct gaagaagtta aggattatgc ggatcgcaat attcatctag
    19381 tccgtaacca agactcgcca tggcgttgtt tcaatgttca tgagaatggc caggaggtgg
    19441 gccatgctta gtttattatc ttacgacttt atacaacgcg cctttttggc ggttattgct
    19501 atgagtcttt tctcaccggt attgggaacc ttcctcatct tgcgtcgtca gagtttgatg
    19561 agtgataccc ttagccacgt ctcactttca ggtgtagcct ttggtctggt tttggggatt
    19621 tctccaactg tttctactat tgccattgtc ttgattgcgg cggtctttct ggagtatctc
    19681 cgtacggttt acaagagctt tatggaaatc gggacagcta tcctcatgtc aacaggtctg
    19741 gctgtttctc tgattgtcat gagcaagggt aaaagctcga gttcaatgag tttggaccaa
    19801 tatctctttg gttcgatcgt gactatcagt gaagaacagg tcatttccct ctttgttatt
    19861 gcggcggttg ttttgatttt gacctttctc tttcttcgtc ctatgtatat cttaactttt
    19921 gacgaagata cggcctttgt ggatggcttg ccagttcgta ccatgtccat tctttttaac
    19981 atggtgacag gggtggctat tgcccttatg attcctgcag caggagctct tctggtatcg
    20041 accattatgg tcttgccagc tagtattgcc ctgcgtctgg ggaaaaactt taaatcggtt
    20101 atgctgcttg ccagtgcgat tggctttttg ggaatggtag caggacttta catttcctac
    20161 tatgcagaaa cacctgcaag tgcaagtatt accattattt ttgtaactgt ctttatacta
    20221 atcagtttag taagacgttt tatcaaatag gagacgaacg tgaaaaaaat tagcttatta
    20281 ctagccagtc tatgtgcctt gtttttagtg gcttgttcca atcaaaaaca ggcagatggt
    20341 aaactcaata tcgtgacaac cttttaccct gtctatgaat ttaccaagca agtcgcagga
    20401 gatacggcta atgtagaact cctaatcggt gctgggacag aacctcatga atacgaacca
    20461 tctgccaagg cagttgccaa aatccaagat gcagatacct tcgtttatga aaatgaaaac
    20521 atggaaacat gggtacctaa attgctagat accttggata agaaaaaagt gaaaaccatc
    20581 aaggcgacag gcgatatgtt gctcttgcca ggtggcgagg aagaagaggg agaccatgac
    20641 catggagaag aaggtcatca ccatgagttt gatccccatg tttggttatc accagttcgt
    20701 gccattaaac tagtagagca cattcgtgac agcttgtcag cagattatcc tgataaaaaa
    20761 gagacctttg agaagaatgc agctgcctat atcgaaaaat tgcaagcctt ggataaggct
    20821 tacgcagaag gcttgtctca agcaaaacaa aagagctttg tgactcaaca cgcagccttt
    20881 aactatcttg ccttggacta tggactcaaa caagtcgcaa tctcaggact ttctccagat
    20941 gtagagccat cagctgctcg cttggcagaa ttgacagagt atgtcaagaa aaataaaatc
    21001 gcctatatct attttgaaga aaatgcctca caagcccttg ctaacacact ttcaaaagaa
    21061 gcaggtgtca aaactgatgt cctcaatcct ttagaaagtc tgacagaaga ggacaccaag
    21121 gctggagaaa attacatctc tatcatggag aaaaacctca aggctttgaa acaaacaaca
    21181 gaccaagaag gcccagcaat cgagcctgaa aaggcagagg ataccaagac agtccaaaat
    21241 ggttacttcg aggatgcagc tgtcaaggac cgcaccttga gtgactatgc aggtaactgg
    21301 caatcagttt atcctttcct tgaagacggc acgtttgacc aagtctttga ctacaaggct
    21361 aagttgactg gtaagatgac ccaggctgag tacaaggctt actatacaaa aggctatcag
    21421 acagatgtga ctaagattaa cattactgat aatactatgg aatttgttca aggtggacaa
    21481 agcaagaaat atacttacaa gtatgtcggt aagaaaattt tgacttacaa gaaaggcaat
    21541 cgtggcgtgc gtttcctctt tgaagccaca gatgctgacg ctggacaatt caagtatgtt
    21601 cagtttagtg accacaatgt tgccccagtt aaggcagaac atttccatat cttttttgga
    21661 ggtacaagcc aagaagctct atttgaagaa atggataatt ggccaaccta ctacccagat
    21721 aacctatctg gccaagaaat cgctcaagaa atgttggcgc attaatgaga aatcgactag
    21781 ttcataagag ttagtcggtt tttgggtact agatggctaa cttcattata gacttttcag
    21841 aactaaggaa ctttaaaaaa ataaataaaa ctcttgaaat atctggactt atatgctaga
    21901 atgtagttag tgtataaggt tcattagaac accaaagccc ctaactagtg ccatagttaa
    21961 gggctttttt gacgtatctt agcgatttaa cgggtgttta taggctagtc agtcggtcta
    22021 cttcttacca tctagccatt tgcagacgaa atacaagata attccagcta taacatctgc
    22081 tataatagta agagcaagct ctggtataag gctcattagt ttcacctcct ctcacgaacc
    22141 gtaaggaagg taatcggtaa ccgatgacaa aagtattata ccacactaaa tttagatcat
    22201 caacctcact taatgcttga actattgtag attatatgct aaaatgtagt taattgataa
    22261 ggttcattag aacaccaaag cccctaacta tggccgtagt taagggcttt tttgacgtat
    22321 cttagcgatt taacgggtgt ttataggcta gtcactcggt ctactcctta ctatctagcc
    22381 atctgcagac gaaatacaag ataattccag ctataacatc cgctataata gtaagagcga
    22441 gctctatgat aaggctcatt agtttcacct cctctcacga acccatagga acgtaatcgg
    22501 taaccgatga caaaaatagt ataccacaat acatttagat catcaaggtc acttaattct
    22561 tgaaatatag ggtctaggcg acaaagtctt taaaatcaga aaaacgcata atatcaggtg
    22621 tgcaaaaact tgatactatg cgttttattg tgggaaggtt tactccattt tctcctgaaa
    22681 ttgagttttt gtccagcctc tgtttttaga attgctaaga aaataatgtc atgtggtgaa
    22741 tatttgtaaa tcagtcagca gacagaacga atactcttcg aaaatctctt cacatcatgt
    22801 cagcttcgtc ttgccgtgtc catgggggac ttgacttcat cagttttcaa atctgtactt
    22861 tgagcaagct gagactagct tcctatttga ttttcattga atatcagaaa cccattctcc
    22921 atcaaataat tcgactgcgt ctaataattt ttgatttggc aaggtgtcag aaataaaggt
    22981 tgtgtatttg gagaggggat taattttaaa aaatccagtc ttgtaaaatt tagaactatc
    23041 aatcagtaag atggtttcat gggctttgtc aataatattc ttttttgaaa tagcttggct
    23101 gagagaagct tcataaacat attggtcatc aatacctctt gctgaacaaa atgctaaatc
    23161 gatattaaaa tgatctaata aagaattttc cttatcatag ttgaccacgg aacaggattg
    23221 atgtttgacc tcgccagatg tgataaagat tttggagcta tctttaacag tttcagatag
    23281 ggtttgtgca gtatgtaaac catttgtaaa aataatcaaa ttatcaagtt cagaaagata
    23341 gggacaaagt tcgtagacag tcgtactgga atccagataa atacacatgc ctgaccgaat
    23401 aaagtcttta gcgagactag cgattagtct tttttgccta gtactttctc cttcccgtat
    23461 ttgatgagaa agttcaattg tgttcataga ggacagggtc acgtatccgt gctttctttt
    23521 gataagacct tgattttcta agaaaattaa atcacgacgt aaggtacttg tgctggagaa
    23581 agtgatttct gccagctctt ttacggcaat tctttttttc tttttaataa tttcaatcaa
    23641 ttcaagtaca cgttcatctt ttatcataag ctcctcctaa tttatcattt caactatatt
    23701 atagcacaaa ttggaggaat gtgaattatt tttatgaata ttgggttaac atttgaacat
    23761 tattcaagta agcgttcaca tattgaaaaa ataaaacgtg gggattataa taaagttaat
    23821 caaggacgaa gagagaagaa aaatggaagc ggttttagca atagatttag gtgcgacttc
    23881 tggaagagca atcgttggtt acctttctga aaataaactg gtaatggaag aaataaatcg
    23941 cttttctaat ctacctatta gagtaaaagg gcatttatct tgggatattg actttctact
    24001 agctaaaatt cttgaaagta tccgcttggc taatactagt tacaagattt tatctatcgg
    24061 tattgacaca tggggagttg attttggact gattgataat gaaggtaagc tgttattaca
    24121 acctgttcat tatcgtgatg aaagaacaaa gggagtgtta aaggaaatat ctgaaatgac
    24181 tgaattagaa aaactgtatt cagagacagg aaatcagatt atggagataa ataccttgtt
    24241 tcaactcttt aaggcacgtc aagaatctcc tgactctttc tataagacca ataagattct
    24301 tttaatgcca gatttgttta attatctctt gacaggtaag ttttctacag aaaaaagcat
    24361 tgcttcaaca actcaattat ttgatcctag gagtcaaaat tggaatcaga atatcttaaa
    24421 actatttgaa ttggattcat ctttacttcc tgaaattgtt tcagagggaa atgttcttgg
    24481 aaggataaaa aaggagtatg gtttaggcga tattcctgtt gtgaatgttt gtagtcatga
    24541 tacagcaagt gcgattgtct cagtacctaa gacagaaggt agtttattta tttcatcagg
    24601 tacttggtct ttggttggag tggaacttac ttcaccgatt cttactaccg aatccttcag
    24661 ttatggattt acaaatgaag tcggtaaaga tggagagatt acatttctga agaattgtac
    24721 agggttgtgg atcatagagg aactaagacg ttcatttgaa cgaagaggga aagcctattc
    24781 ttttgatgat attaggacaa tggtggagaa agaaaaagaa aatcttcctc tgattgatac
    24841 tgaatcaact gaatttgcaa cagaatctga tatgcacaag actttgacag aatatctagc
    24901 ttatcatcat gaaactagag agtggacaga tggacaacta tttaagattg tttatgaaag
    24961 cctagctgaa acgtatagga aagcgataga gttactagaa gaactaactc ataaggttta
    25021 taagaggata tatgtgattg gaggaggtgc tagagccagt tactttaacc aaatgattgc
    25081 tgatagaact ggtaaagagg ttcttacagg ttcgactgag ggtacagctg tggggaatat
    25141 tgttgtgcag ctcatagcta tgggacaatt aaaagggatg gaagaagctc accatgttat
    25201 tgaggagttt ctacaattag agagttatta ctcccaaaag aattaaaaag attgagagtt
    25261 tgtaaatttg cctccctccc ccttcttaag ttttgtacaa gaagggggga taattggtga
    25321 attgaaaaat atttagtgtt ttgatatgag gaggacaaga atgtcagatg taaaacaaga
    25381 attaattaag tatggcaaga agctagtaga aacagatttg acgaaaggaa caggtgggaa
    25441 tctcagtgtt ttcgatcgtg aaaaacaatt gatggcaatt accccgtcgg gtattgattt
    25501 ctttgaaatc aaagaatccg atattgtagt gatggatatt aatggaaatg ttgtagaggg
    25561 agaacgcttg ccatctagcg aatggtatat gcatttgatt caatatcaaa ctcgtgatga
    25621 tatcgatgca attatccatg ctcatacaac ttatgcaaca gtattagctt gtctcagaga
    25681 accacttccg gctagtcatt atatgattgc agtggcaggg aaagatgttc gggtagctga
    25741 gtatgcaaca tatggtacga aagaattggc tgtgaatgca gctaaagcaa tggaaggtcg
    25801 tagagcagtt ttactagcga atcatggaat tttagcaggt gcacaaaatt tattgaatgc
    25861 atttaatatt gttgaagaag ttgaatattg tgcaaaaatt tattgtttag ctaagaattt
    25921 tggagagcca gtagttcttc ctgatgagga gatggattta atggcagaaa aatttaaaac
    25981 atacggtcag agaaaatagg gaggatatta atgttaaaac atataccgaa aaatatttct
    26041 ccagatttat tgaagacttt aatggaaatg ggacatggag atgaaatagt attagctgac
    26101 gcgaattatc cttctgcctc atgtgcaaat aagctaattc gttgtgatgg cgtaaatatt
    26161 ccagaattat tagattccat tctgtattta atgccattag atagttacgt cgatagttca
    26221 attcagttta tgaacgttgt ttcgggtgat gatattccta agatatgggg tacctataga
    26281 cagatgattg aaggtcatgg tacagatctt aaaacgatta cttatcttag aagagaagac
    26341 ttttatgaac gtagtaagaa agcttatgct attgttgcta caggagaaac ttcactttat
    26401 gctaatatta tccttaagaa aggagtagtt gttgaaagag aaaatgttta atagaggaat
    26461 tttagttgcc agtcatggta attttgctag cggagctctc atgaccgcag aaatgtttgt
    26521 tggtgagaca acaaatgata gagttaggac attaggtttg atgcctggag agaatattgt
    26581 agagtttgaa cattatttta aaaatcaagt aaatgaactg ttagactcaa atcaagaggt
    26641 tatcgttttg actgacttga ttggaggaag tcctaataat gtggctttgt cacggttttt
    26701 aaatttggat tcagttgata ttgtaacagg gtttaatatc cctctcctag tggaattaat
    26761 atcaagttat gattcaaaaa tcaatttaga agaaattgtt caaaatgctc aaaatagttt
    26821 gtttaatgtt aaacaacaac ttaacgtaga ggaggaagag gatttatgtc tatagagttt
    26881 gttcgtattg atgaccgtct ggtacatggt caagttgtca ctacgtggct aaaaaagtat
    26941 gatattgagc aagttatcat tgttaatgat cgcatctcag aagataaaac acgacaatct
    27001 attttaaaga tttctgcacc ggtaggttta aaaattgttt tctttagtgt aaaacggttt
    27061 gtggaagttt taaactctgt gccaataaaa aagagaacaa tgctgatata tacaaatcca
    27121 aaagatgtgt atgattctat tgaaggaaat ttaaaattgg agtacctcaa tgtaggacag
    27181 atgagtaaaa cggaggaaaa tgaaaaggta acgggaggtg tagctctagg tgaagaagac
    27241 aaatattatt ttaagaaaat agttgataag ggaacgagag ttgaaattca aatggttcct
    27301 aatgataaag ttacaatgtt ggaaaaattt ttataaaaat aatttaagga ggtacagtat
    27361 atgctattca cacaagcatt actggtgaca ttagttggga ttattgccac tattgactat
    27421 aatggaccgt tatttatgat tcaccgtccg ttagttacaa gtgcaatggt tggcttagtc
    27481 ttaggagatt tcacccaagg tgttcttatt ggttcagctc ttgaattaac ttggctcggt
    27541 gtaacaggta ttggaggtta cactccacca gatactattt caggtgcgat tattggtact
    27601 gcatttggta ttttgtctgg tcaaggagaa actgctggta tcgctatagc agttccaatt
    27661 gcagttgcta ctcaacagtt ggatgttctt gcaaaaactt tagatgttta ttttgtgaaa
    27721 aaagctgata atgatgctaa aaacggagat tattcaaaga tcggttttta tcattattca
    27781 agtttggttt taatcacttt atttaaaatt gtaccaattt tcctagctat tatgcttgga
    27841 ggggaatatg tggcagactt gtttgctaag gttccaccaa tcgttatgca gggacttaac
    27901 tctgcaggtg ctttactacc ttcaattggt tttggtatgc ttttaaatat gatgctcaag
    27961 aaaaatatgt gggtattctt gttgattgga ttcatttgtt ctgtgtatgg aggaatgtca
    28021 accattggga tctcactagt tggtattgcg gtagcatact tctacgatat gattggaagt
    28081 aaaccacaag aaacaacttc aagtagtgat gttgaggagg atcttgatct atgatgaata
    28141 ataaagtaac taaagttgaa cttaaaaaag ttttcaaacg aagttttatg tatggttctt
    28201 catggaacta tgagagaatg cagaacctag gttttctata tacaattctt ccagtattga
    28261 aaaaactata cccagacaaa gattcagctt ctcctgcaat gaaacgtcac cttgagtttt
    28321 tcaatactca tcaaacagcg gcaccattta ttcttggagt tacttccgct atggaagaac
    28381 aagaaggaaa tgaaggtgca gcttcaatta ctggtattaa agttggcttg atggggccac
    28441 tggctggtct aggagatagt ttgttctggc tgacactagt tcctatctgt tttagtattg
    28501 gtgcgtctta ttctaaagac ggcggtgctt taggtatctt tatcgcctta atattgttta
    28561 atattattaa tattcctgtt aaatatttcg gtttgaaata tgggtatact aagggttcta
    28621 gtcttatcca agaaaataat acaaaaggaa cattgaatcg cgttacgagt atggcgacag
    28681 cattagggct agtactagtg ggtggtttga ttccatcaat ggttggtatt aattttggat
    28741 tagaatttaa gcagggggaa cttgttattt ctgttcaaga aatgattaca aaattaattc
    28801 caggatttat ccctatggct ttgactttat taatgtgtaa attaattaga aaaggaaaga
    28861 atccggttgt actaatcttt agtgttatgg ctattggagt tatcctagtt gttttaggaa
    28921 ttttgaagta gtagaaagtg tggaggtggt atttgggata tcacctccat tttggaagag
    28981 aggtaaagag tgaaattatg gtataagaaa gctgccgcaa attggaatga agccttgccg
    29041 attgggaacg gtcatttagg tggtatgatt tatggttcag ctacaaaaga atgtattcaa
    29101 ctaaacgatg agactatttg gtatagagga aagtcagata gaaataatcc agactcacta
    29161 ttgcatctta aaaaaattcg ggaatatctt ttagatggag aaattcagaa agccgaagaa
    29221 ttgataaagt taacaatgtt tgctacccca agagatcaaa gccactatga attacttggg
    29281 gaactttgca ttgagcatat agatattcag tcttgtgctc tttcattgta tgaaagagag
    29341 ctagatttag atacagctat ttctaatgtt gtgtttgagc ctaatagttg taatttacaa
    29401 ataaaaagag aatattttac gagttttaat aagaatattt tatgttgccg tatagtgtca
    29461 tcagttcaaa acacattaaa tttaaacatt aatttgggta gaaataaacg gtttaatgac
    29521 gaagtatcta aactggattc aagtacaatt ttaatgtcgg cctctgctgg aggtagaaaa
    29581 ggtgttcagt ttaaagtagt atgtcattct aaggttacgg atggtgaagt aagtgtattg
    29641 ggagagacaa tagttattcg gaatgctaca gaggtatttc tttatctcaa atcaatgacg
    29701 gattattggg gaaatataga tatttcttct cttcagggag aatttagtag tattgattac
    29761 tttacagaaa aagatgaaca tgtaaaaaaa tatcaggagc aatttaatag agttgatttt
    29821 aaactagact atagtaaagg ttgtcttagc attccaacga atctacttct tgaaaacact
    29881 aaaaagtata gtaactactt gactaacttg ttatttcatt atggaagata tctgttaata
    29941 tcgtctagtc aaccgaatgg tttacctgcc aatcttcaag gaatatggtg tgatgaatta
    30001 aatccaattt ggggttctaa atatacgatt aatattaata ctcaaatgaa ttattggatg
    30061 gtaggtccat gtgatttacc agaagtagaa tatccattat ttgatatgct cgaaagaatg
    30121 agagaaccgg gaagactaac cgctaagaaa atgtatggag ctagaggttt tacagcacat
    30181 cataatacgg atggttttgg cgatacggct ccccaatctc atgccatggg ggctgcaatt
    30241 tgggtattaa ctattccatg gttatgtact catatttggg aacactattt atatttccaa
    30301 gatgagcgta ttcttacgga acattttgaa atgataaaag aagcatttct tttctttgaa
    30361 gattatttat ttgaggtgga tggctacttg atgacaggtc caagtgtctc accggaaaat
    30421 aaatatcgct taaaaaatgg tattgaagga aatgcttgtc tatcatctac aattgataat
    30481 caaattctaa gatatttttg tgattcatgc attggcattg caaaacaatt aggagacaat
    30541 tcggatttta ttagtcgtgt gaaggagtta aaaaagaaat tacctagaac aaaaataggt
    30601 agtaatgggc aaatacaaga atggctagag gattatgaag aagtagagcc tggtcataga
    30661 cacatttcac ctctatttgg gctttatcct tataatgaga ttgatattca taaaactccg
    30721 gaattagcag aagcagctaa aatcactatc aataggagat tatcaaacgc taacttttta
    30781 tcttcacagg atagggagca agcgattaat aattggttag taagtggttt gcatgctagt
    30841 acacaaacag gttggagtgc tgcatggctg attcattttt ttgcgagact atatcaaggt
    30901 gaacctgctt ataaccagat taatggtttg ttaaataatg cgactcttgg caatttattt
    30961 cttgaccatc caccatttca aattgatggt aatttaggtt tggtgagtgg aatttgtgaa
    31021 ttattagtac agagccatca taattggtta tcactaattc cagctttacc ttctgcttgg
    31081 tcagaaggag aagtgaaagg tttcagagta agaggaggat ataaggtatc gtttgcttgg
    31141 aaaaatgggg atataacatt cctaaaattg gaaggaggaa acaaagatca aaaagtaaga
    31201 gtaagaatat atggcaaaaa tactgatgta caaaatattg aattggtatt taattcagaa
    31261 aaaattattg agttaaattt ttaggtataa gtcatgaata aagaaaaaat aaaaagaaaa
    31321 ttaatcacaa tattgtttgt atgtattggg atgttatgtt ttggattgtt agcaggagtt
    31381 aaggctgata atcgtgttca aatgagaacg acgattaata atgaatcgcc attgttgctt
    31441 tctccgttgt atggcaatga taatggtaac ggattatggt gggggaacac attgaaggga
    31501 gcatgggaag ctattcctga agatgtaaag ccatatgcag cgattgaact tcatcctgca
    31561 aaagtctgta aaccaacaag ttgtattcca cgagatacga aagaattgag agaatggtat
    31621 gtcaagatgt tggaggaagc tcaaagtcta aacattccag ttttcttggt tattatgtcg
    31681 gctggagagc gtaatacagt tcctccagag tggttagatg aacaattcca aaagtatagt
    31741 gtgttaaaag gtgttttaaa tattgagaat tattggattt acaataacca gttagctccg
    31801 catagtgcta aatatttgga agtttgtgcc aaatatggag cgcattttat ctggcatgat
    31861 catgaaaaat ggttctggga aactattatg aatgatccga cattctttga agcgagtcaa
    31921 aaatatcata aaaatttggt gttggcaact aaaaatacgc caataagaga tgatgcgggt
    31981 acagattcta tcgttagtgg attttggttg agtggcttat gtgataactg gggctcatca
    32041 acagatacat ggaaatggtg ggaaaaacat tatacaaaca catttgaaac tggaagagct
    32101 agggatatga gatcctatgc atcggaacca gaatcaatga ttgctatgga aatgatgaat
    32161 gtatatactg ggggaggcac agtttataat tttgaatgtg ccgcgtatac atttatgaca
    32221 aatgatgtac caactccagc atttactaaa ggtattattc ctttctttag acatgctata
    32281 caaaatccag ctccaagtaa ggaagaagtt gtaaatagaa caaaagctgt attttggaat
    32341 ggagaaggta ggattagttc attaaacgga ttttatcaag gactttattc gaatgatgaa
    32401 acaatgcctt tatataataa tgggagatat catattcttc ctgtaataca tgagaaaatt
    32461 gataaggaaa agatttcatc tatattccct aatgcaaaaa ttttgactaa aaatagtgag
    32521 gaattgtcta gtaaagtcaa ctatttaaac tcgctttatc caaaacttta tgaaggagat
    32581 gggtatgctc agcgtgtagg taattcctgg tatatttata atagtaatgc taatatcaat
    32641 aaaaatcagc aagtaatgtt gcctatgtat actaataata caaagtcgtt atcgttagat
    32701 ttgacgccac atacttacgc tgttgttaaa gaaaatccaa ataatttaca tattttattg
    32761 aacaattaca ggacagataa gacagctatg tgggcattat caggaaattt tgatgcatca
    32821 aaaagttgga agaaagaaga attagagtta gcgaactgga taagcaaaaa ttattccatc
    32881 aatcctgtag ataatgactt taggacaaca acacttacat taaaagggca tactggtcat
    32941 aaacctcaga taaatataag tggcgataaa aatcattata cttatacaga aaattgggat
    33001 gagaataccc atgtttatac cattacggtt aatcataatg gaatggtaga gatgtctata
    33061 aatactgaag ggacaggccc agtccctttc ccaacaccag ataaatttaa tgatggtaat
    33121 ttgaatatag catatgcaaa accaacaaca caaagttctg tagattacaa tggagaccct
    33181 aatagagctg tggatggtaa cagaaatggt aattttaact ctggttcggt aacacacact
    33241 agggcagata atccctcttg gtgggaagtc gatttgaaaa aaatggataa agttgggctt
    33301 gttaaaattt ataatcgcac agatgctgag actcaacgtc tatctaattt tgatgtgatt
    33361 ctatatgaca ataatagaaa cgaagttgct aagaaacatg ttaataattt gtcgggtgaa
    33421 tctgttagtc tagatttcaa agaaaaagga gcaaggtata ttaaagttaa attactaacg
    33481 agtggagtgc ctttgagttt agcagaagta gaggttttta gagaatcaga tggtaagcaa
    33541 tctgaagagg atatagataa aataacagaa gataaagtag tctctacaaa taaggtagct
    33601 actcaaagtt caaccaatta tgagggtgta gctgctttag cagttgatgg taataaagat
    33661 ggagattacg gacatcattc ggtgactcat actaaggcag attctaacgc ttggtggcag
    33721 gtcgatctgg gagaagagtt tacggtttct aaagttgata tttataatag aacagatgcc
    33781 gaacctcagc gtttatctaa ttttgatgtt atttttctat cttcatcagg agaagaagtt
    33841 tttagaagac attttgataa agtagttgat ggtttgttat ctttaaaagt accttctgta
    33901 ggggctaagc tagtcaaaat agaattaaaa tcagcagcta ttccgttaag tttagcggaa
    33961 gttgaagtct atggttcaaa gagaactccg aagaaacttt ctaatattgc attaaccaaa
    34021 gaaactcgac agagttcaac ggattacaat ggtttttctc gtctagcagt tgatggaaat
    34081 aaaaacggag attatggtca tcattcagtg actcatacca aaggagattc tccttcatgg
    34141 tgggagatag atttagcaca aaccgaagaa ttagaaaagt taattattta taatagaaca
    34201 gatgctgaaa ttcagagatt atcaaatttt gatattatta tatatgattc aaataatcat
    34261 gaagttttta aacaacatat tgacagttta gaaagcaata atctatccat agacttaaaa
    34321 gggctgaagg gaaaaaaggt tagaatttct ttgagaaacg caggaattcc tttaagtttg
    34381 gcagaggtag aggtttatac ttataagtaa ttttaaaaat tatcatcggt agtatgaaag
    34441 aaacagaaaa ataagaggaa aatagtatga ttcaacatcc acgtattggt attcgtccga
    34501 ctattgatgg tcgtcgtcaa ggtgtacgcg aatcacttga agtacaaaca atgaacatgg
    34561 ctaaaagtgt ggcagatttg atttcaagca cattgaaata tccagatggg gaacctgtgg
    34621 aatgtgtgat ttctccatct accattggtc gtgttccaga ggctgcagct tcccatgagt
    34681 tgttcaaaaa atcaaatgtt tgcgcaacaa ttacagttac accatgctgg tgttatggta
    34741 gtgaaactat ggatatgtct ccagatattc ctcatgctat ttggggattt aatgggacag
    34801 aacgcccagg agctgtctat cttgcagctg tactagcttc acatgctcaa aaagggattc
    34861 cagcctttgg gatttatggt agagatgttc aggaagctaa tgatacagat attccagaag
    34921 atgtcaaaga aaaactttta cgttatgcgc gtgcagctct tgcaactggc ttgatgagag
    34981 acactgctta cctatcaatg ggtagtgttt cgatggggat tggcggttct attgtaaatc
    35041 cagatttctt ccaagaatac ttaggaatgc gaaatgaatc ggtagatatg acggagttca
    35101 cgcgccgtat ggaccgtggt atttatgacc cagaagagtt tgaacgtgcg ctcaaatggg
    35161 tgaaagaaaa cgtaaaagaa ggattcgacc ataaccgtga agaccttgtt ttaagccgtg
    35221 aagaaaaaga tagacaatgg gaatttgtta ttaagatgtt catgattgga cgtgacttaa
    35281 tggttggtaa cccaagactt gctgaacttg gttttgagga agaagcagtt ggtcaccatg
    35341 ctttagtagc tggtttccaa ggtcaacgtc agtggacaga ccattttcca aatggagatt
    35401 ttatggaaac tttcctcaat actcagtttg actggaatgg tattcgaaaa ccatttgtat
    35461 ttgcgacaga gaatgattca ctaaatggtg tgtctatgct ctttaattat ctattaacaa
    35521 atactccaca aatatttgct gatgtgcgta cttattggag tccagaggct gttaaacgtg
    35581 taacgggaca tactttagag ggtcgtgctg cagctggctt cttacatcta atcaactctg
    35641 gttcttgtac attggatggt acaggtcaag ctactcgaga tggcaaacct gttatgaaac
    35701 cattctggga gttggaagaa agtgaagtgc aggctatgct tgaaaataca gacttcccac
    35761 cagcaaaccg cgaatacttc cgtggaggag gattctcaac tcgtttcttg acgaaggggg
    35821 atatgccagt aacaatggta cgtctcaatc ttctaaaagg ggttggtcca gtgctacaaa
    35881 ttgcagaagg ttatacactt gaacttcctg aagatgttca ccatacttta gataatcgta
    35941 cagatccagg atggccaact acttggtttg ctccacgttt gacaggaaaa ggtgctttca
    36001 agtctgtcta tgacgtcatg aataattggg gagctaatca cggagccata acatatggac
    36061 acattggagc agacttgatt accttggctt ctatgttgag aattcctgtc aatatgcata
    36121 atgtacccga ggaagatatc tttagaccta aaaattggtc cttatttgga acagaagatc
    36181 tagaatcagc agactatcgt gcatgtcagt tgttgggacc gctacataaa taaaaattgt
    36241 gtatatagga ggtgaactta tgtccctcct atccttttaa aaagatttgt taaacaattc
    36301 acaaataatt gaaaacgaat acaaaaacta atataatgat gttaaataga tagcgcggag
    36361 gcgcaggagg aaaattatat ggctacattt tatgttccgg cagtcaacct tattggtaaa
    36421 ggtgttgtaa atgaagtggg tccttatatc aaggaacttg gctataaaaa ggcacttttg
    36481 gtgacagata agtacatcga aggcagtgac attttaccta aggttttaaa accactggat
    36541 acagaaggaa tcgaatatgt catctttagc gatgtagagc caaaccctac ttgtaaaaat
    36601 gtcacagatg gggtagctgc tttgcaagaa catggctgtg actttatcat cagtcttggc
    36661 gggggttctc cacaggatgc agctagttgt atttctatca tggctacaaa tggtggaaaa
    36721 ccacaggatt atgaagggct tcataagtct gctaaaaaag gcttgccagt tgtggctatc
    36781 aatacaacgg caggaacttc tgcagaaatc actattaact atgtgattac tgatgaagaa
    36841 cgcaaggtta agatggtaat ggttgacaag aatagccttg ctcttatctc tgttaatgac
    36901 ccggaactca tgctttccaa acctaaaggc ctgactgctg ctactggtat ggatgctctg
    36961 actcacgctg ttgaagcttt ggtaacacct ggtgcttatg atgtaaccaa gaaactgtct
    37021 attggtgcta ttgagcttat caaggaatat cttcctcgtg ctgtagaaaa tggacatgat
    37081 attgaagcgc gtgaaggtat ggtcaatgcc atcttccttg gtggtatgag ctttaataat
    37141 gctggtcttg gttatgttca ctcaatggct caccaactcg gtgcagtata taatttgcca
    37201 catggtgttt gctgtgccat gttgctacca gttatagaac gtgaaaatgc taaacgtgta
    37261 ccagaagctt tccgcaatgt tgccaaagcc ttgggacttc atgtagaagg taaatcagat
    37321 caagaatgtg ccgattatgc gattgttgag attgagaagc tttctgagac agtaggtatt
    37381 cctaagaaac tgactgaact tggtattgaa gaaaaagatt tcgactttga atacctttct
    37441 aagaatgcct tgattgatgc ctgcgcacca ggaaatccat ttatgccaac cttagaagaa
    37501 acgattgcct tttataaaga gttattttag caagagattg gacgacaata ggacaattca
    37561 cctctaatta ttgacatttc aggaaaaatc gcagtaaaaa cggcacagcc ttctttaaat
    37621 gtgctataat actagaaaaa tacttgtgga ggttccatta tggcaatatt tttcatgatt
    37681 tttctgattg tttgtgtgct cctattggtg atagtcacac tgagtacagt ttatgtggtt
    37741 cgtcagcagt cggtggcgat tattgaacgc tttgggaaat accaaaaggt tgctaatagc
    37801 ggtattcata ttcgcttgcc ttttgggatt gactcgattg cagcacggat tcagttgcgc
    37861 ttgttgcaaa gtgatattgt ggttgagact aagaccaagg acaatgtgtt cgttatgatg
    37921 aatgtagcga ctcagtaccg tgtcaacgag cagagcgtga cagatgctta ctataaactc
    37981 atacgtccag aatctcagat taaatcttat atcgaagatg ctcttcgctc ttctgttcca
    38041 aaattaacct tggatgaatt gtttgagaaa aaagatgaga ttgcccttga agttcaacac
    38101 caagtagcag aagaaatgac cacttacggc tacattatcg tgaaaacctt gattaccaaa
    38161 gtcgaaccgg atgcagaagt taagcaatcc atgaatgaaa tcaatgcggc gcaacgtaag
    38221 cgggtcgcag cacaagaatt ggcggaagct gacaagatta aaattgtcac tgcagctgaa
    38281 gcagaagcag aaaaagaccg ccttcatggt gtggggattg cccaacaacg taaggcgatt
    38341 gtggatggat tggcagagtc tatcaccgaa ctcaaggaag ccaatgttgg catgacagaa
    38401 gaacaaatca tgtctatcct cttgaccaac cagtatttgg ataccttgaa tacctttgcc
    38461 tctaaaggaa atcaaaccat ctttttacca aatactccaa atggtgtgga tgatatccga
    38521 acacaaatct tgtcagccct tcgcgctgag aagaaataat agactaatac tcttcgaaaa
    38581 tctcttcaaa ccacgtcagc gtcgccttgc cgtatagatg ttactgacga agtcagttct
    38641 atctgcaacc tcaaaacagt gttttgagct gacttcgtca gtcttatcta ctacctcaaa
    38701 acggtgtttt gagcaacctg tggctagttt cctagtttgc tatttgattt tcattgagta
    38761 caaagagctt ggcaacaggc tctttttgta ttgcgtttat taagaaaaac aaaaaacatc
    38821 gatatacatt taactaaaaa ttattgtatg ttatctccct taattaggaa tgataaggga
    38881 ataactagaa agatttgtga atacaaacta tttctgatat acaaaatata cagtaataat
    38941 gaatgatggg agatgggatg aaagaatttc aatttgagag aaagcagcgt ttttctttga
    39001 ggaaatatgc aataggagct tgttcggtct tgctaggaac gagtttattt tttgctggta
    39061 tgggtgctca gcctgtacag gctacagaaa cgagttcagc actaatttca agtcattatt
    39121 tggatgagca ggatttatct gaaaagctga aatctgagtt gcaatggctt gaattagaaa
    39181 acaagctttt gaacttatgg gagcattagg ttactatgaa ggatttgttc cttacgtttc
    39241 aaattaatac aaaaaccaag ctgaagaaga caaaccgcta tctgataaat atattttcga
    39301 aaaaatctta ggaaaaacat atgcagcctt caaaaaagat caaattaatg agcgtgtcga
    39361 gaaattaggt aagttaaaac ctattacaat aaattacaac ggaaaatcag aagtaattga
    39421 tagtaaagaa aaattacaag agcttatgaa taaagccgtt aaagacgaag tggctcaaat
    39481 atagtaaaat gaaataagaa caggacaaat cgttcaggac agtcaaatcg atttctaaaa
    39541 atgttttaga agtagaggtg tactattcta gtttcaatat actataaaat caggcaatac
    39601 cacggctaaa aagtttgagt ttatcgaaac accagtgcaa aaactgaaaa aagcgattta
    39661 taaagctcat ctcaaagatt cagatgactt tagaccagaa acatccacac aaatcttttt
    39721 gaaagctgtt tgaagctttg tccttgcttt cttagttcat agatctgaac tttatcttca
    39781 taactcaatt tcataataaa acaccccaaa agttagattt attctgtcta acttttgggg
    39841 tgcagttcat taatcggtcg attttttttg gtagaaacag ttgcctgatt aagttgcaag
    39901 tcgatagacg gcttcggcat aaatatccat agcacggtac aaatcttcta gaacggcaca
    39961 ttcatttgcc tgatgttctg tctgcttcgc tcctgggaat aaggcgccga aggctacaca
    40021 atttggcatg gtgcgagcaa aagtggcacc accggatgaa atagcaggac tgttatcgcc
    40081 agtcttttct tggtagattt gcatcagtgt gctaacgagt ttactgtctt ttgcgacgta
    40141 tagaggcgct agatagtcaa actcttcgta gcggagttgg tagttttgtg cacatcttgt
    40201 aagcaactct actagttctt ccttgtcagc taagacagga gtccgaatgt caatacggat
    40261 ttcagaacgt tcatgattga tcatgagacc tgcgacatta aaggataggt gaccagaagg
    40321 ttcatctgct atatcaccaa agatttgtct tcctgtgccg tcttgacctg cttgtgttgc
    40381 aagaaagctg agagcagggt gttcttggag aggagcaaga atggtagcta gtcggatgac
    40441 agcattgata ccttgactag catccttagc atgctttggc actccgagaa ccgttacggt
    40501 ttgttcagtg gattggtaat cataaccagc ttctttgaga tcgttacaaa cctgttcata
    40561 gaggggacct tggtagttgg ccttgcctgg tacaacgtta aaggcgcctc ctacttcaag
    40621 ctctagttga tccgatccag ggccatgaag tttgacctgt agaagccctt tttcagcata
    40681 ggtcagagga aaagatgagt caggtgcaaa gcccatactg gcctgttctt cgatggtatt
    40741 gtagcgtgcc atgcagcgcc agagggtttc ctcatcggta ccaaagataa agcgtacgcg
    40801 ctttttgaac tgaatacctt ggtccagcaa gctttttact gcatagagag ctgcgagcga
    40861 agggccttta tcatcttgga caccacgtcc gaatacccag ccgtctttga tagttgcttc
    40921 aaatggcggt gtctgccaat ctgcttcatc acctgatgga acaacatcca aatgacagag
    40981 aatggccaga agctctgctc cctgaccgat ttctgcatat ccgtaataac ctttagggtc
    41041 aagataggta gtgaaaccta tgtctcgaca aatctctaaa gttttttcta ggacatcttg
    41101 gattgcttgt ccaaaaggtg ttccattttc tccttcattg agtactgaag gataggaaat
    41161 caaggttttt aatgatataa gaaattcatc tttaacttga tttgttatat ctattttcat
    41221 ggaatcacct cactcactat tttataggaa tggaaggaag gttccaagga gaagaagggc
    41281 gatggtcact acaataatag cgactacgag tttgcccatg aatttccacc aagtaccaat
    41341 gttgatacgt ccaagtgcaa gagctcccat cacaatacca gatgttggtg caatcaagtt
    41401 caagacacct gaagcagatt ggtaagcagt gataatcaag ctaggacgga catttacaaa
    41461 ttctccaagt ggagccatga tacccatagt tgcgctggca agaccagatg aagatgggat
    41521 caagaatgac ataggtagat agaagatata agttacaacg ataaagactt gtgaagatag
    41581 accgctcaag ccttctttac cccagttgag gattgtatcg gtaatcatac cgtcgttcat
    41641 gataacttga ataccacgag caatcgctac gatcaaggca acactgagca agtcagcagc
    41701 accattcatg aaggaagaga taatcttatc ttctttaaga ccataaataa caccaatcag
    41761 gatacccata aaggcaaaga gcattgcgcc ttctgggaag taccaagtac ctagtgcaga
    41821 agtagatgaa ccgacaatat taccaataac tggaagacca gtcaaccaag cattaaagtc
    41881 atcaaaaatg gtaacgccaa ggtctgtcca tggaatgaag ctcaatacca tcaagatgaa
    41941 tgtcaacaca aataagaaga gaactgattt ttgtttgctg ctaagtgtag attctacaga
    42001 tgaagattct tctacgttaa agtgtttcaa atcttctttg cgagtactat aaaccagtga
    42061 cttagtcgga tctttttgaa tcttatccgc ataacggtaa acaaaccaag tactaagagc
    42121 agtcaaggta acccagaaga tcagacgaag tacgataccg tcccctgtac caactcccgc
    42181 agtcgctgaa gcaatacctg tcgcaaatgg attcagagta gatgccaaac agccgatttg
    42241 agaaccgagc aaaataattg caacaccagt caggctatca aaaccaacgg ccatcataac
    42301 tggcacaagg agtggataga aggccattgt ttcttcaccc ataccataag ttgtaccacc
    42361 gagggcaaac aaaggcatca gtaccaaaat taacattttt tcgcggccct tatacttctt
    42421 cacgatagag gcaatcccta cgtcaagagc accagttttg ttgacaatgc caaggaaacc
    42481 accaaccata aggatgaaga aggctacatc aatcgctgcg ctcgtttctt taatgagcga
    42541 accttcctct ggatgagtac ctagcatagc ccgaatcggt gccatgagga catcccaaat
    42601 cccttgtgga ttttgaggct gagtctcgta aataccttct ataaaggccc ccgcagggat
    42661 aaaccaagtt agcactgcca taatagcaat gattatcaat aatacggtgt aagatgaagg
    42721 catcttaaac ccttttttag ctttttcact catttgtttt tcctcctgat gtctaacaaa
    42781 gtgtaaaaaa ccaactactt tcttaaataa acattcaaga aaacgctttc tatttatatt
    42841 tgttttacca tattaaagtc taaaactagt atcttttgaa atattgtaca tgttttcaag
    42901 aataatttct aatttgatat aaagatacta gaaatagaat acatcttatt agtaaaacaa
    42961 cttatccttt ttcaataatt gttccgcttt cagattcaat caaggcgcct agattttcaa
    43021 gggaagtaat aactgctttt ccttctggac gaccattgac aaaagcgata gctgcttcta
    43081 cttttggaag catgctacct ggtgcaaact gatcttgttt gatatattct tccagctggg
    43141 caacattcac atgttccaat ttttcctggt ttggcttgtt gtagttaaca aatacataat
    43201 ctacacctgt caaaacgatg aagaggtctg catcaaccaa ttctgccaaa cgttgggaag
    43261 cgaagtcttt atcaataacc gcttcgacac cagtcaaatg tccattgttt tctttgacga
    43321 cgggaatacc gccaccacct gcagctacga cgacttgacc attatttaaa agagtacgga
    43381 tggtttcaat ttctttgatg tcaacaggtt ttggtgaggc aacgacctta cgccagccac
    43441 ggccagcatc ttccttgaaa gtcgctccgc tcttttcggc ttctgctttt gcttcttctt
    43501 ctgaatagaa aggaccgatt ggtttactca agttaacaaa agctggatca tttttatcta
    43561 cgacaacttg cgttacaaca gaggcaacat ttttttcgat gccttcatcc aagagagcat
    43621 tttgcaaagc atttttcaac cagaaaccga tgctaccttc tgtcatagcg acaagtgagt
    43681 cgagtgggaa ggcagggttc ttttcagagt ctgatgccaa atgttggagc aagagattcc
    43741 caacttgagg tccattaccg tgagtgataa tcaaatcatc tccattttta atcaatttta
    43801 caagatgctt agctgtttca actaaagctt cttgttgagc ctttgctgat gggtcagaag
    43861 aaagaatcgc atttcctccc aaagctacta caattttacg atttgccata aattctcctt
    43921 tatcacactc aatagaatgc gtttagattt caatttaatg atttttcaca tattttataa
    43981 gaaataatag attaccatta tataaaagag gaccggacta aagctattag tcgcagccct
    44041 catagctgtt ggtagacggt ttattatcta aaattatact ttaggaatat aaaggttacc
    44101 aagtgtagca gccataacag ctttgatagt gtgcatacgg ttttctgctt gatcgaagtg
    44161 gcgagcgtac ttgctgcgga agacttcgtc tgttacttcc atttcttcta caccaaattt
    44221 ttcagcaacg tctttaccat aaacagtgtg agtatcgtgg aatgctggca agcagtgtag
    44281 gaagatcaag ttttcattgc ctgctttttt aactaagtcc atattgactt ggtaaggttt
    44341 aagaagagct acacgttctg cgaatttgtc ttcttcaccc attgataccc aaacgtctgt
    44401 gtaaagaacg tctgcatctt taactgcttc atcagcatct tcagtgatga gaacatgtgc
    44461 gccactttct ttagcaaatc cttctgccaa ttcaacgatt tctttttctg ggaagagttc
    44521 ttttggtgag aagatgtgaa cattgacacc aaggatagca cctgttacga gcaagctgtt
    44581 ggcaacgttg ttacgtccat caccacagta taccaatgtc aagccttcca agcgaccgaa
    44641 gttttcttga acagtcaagt agtcagcgag catttgagtt gggtgccatt cgtcagttag
    44701 accgttccat actggaacgc ctgagaattc tgccaattct tcaaccatac gttggctgaa
    44761 tccgcggaat tcaatcccgt caaacatacg tcccaatact ttagcagtat cttcagtaga
    44821 ttctttttta cccaactgaa tatcatttgc tccgaggtat tctgggtgag caccaaggtc
    44881 gatagccgca gttgtaaagg ctgcacgagt acgagtagat gttttttcaa ataggagagc
    44941 gatattcttg ccagcaaggt agtggtgttg aatattgcgt tttttcaaat ctttcaagtg
    45001 agctgaaaga ccaataaggt attctaactc tgcacgggta aagtcttttt ctgctaagaa
    45061 gctgcgtcct tggaatactg aatttgtcat tttattattt cctctttcta ttttttacat
    45121 tttctattga cgaataccga acagcgatta cacttcttca cgttcaaatg gcatagacat
    45181 acaacgaggt ccaccacggc cccgaaccaa ttcacttccg cgaatcttaa tcaagcgaag
    45241 cccgtattct tccaaaatct tattggtcac ggtattgcgg tcataaacaa ctaccacacc
    45301 aggtgcgatg gtcaaagtgt tagaaccgtc gttccattgt tcacgcgcag ctgctacgat
    45361 attgccacca ccgcaacgaa tcaaatgaac tttttctaca ccaaggtttt gagcaagaag
    45421 ttcagctaag tcacctttct cttcaacgat tttaagtttt tcgttttcgt aagtaactga
    45481 gtaaacgtga aggtcgcctt cgatttctgg gtgaatagtg aacttgtcat agtctaccat
    45541 agtgaagaca gtatccaagt gcatgaattt acggttgtta gcaaattcaa aggccaaaac
    45601 tttcttgaag ccaacatttt tcttgaagat gttgaccaaa agtttttcga tagaagctgc
    45661 gtctgtacgt tgagagatac ctactgcaag gacgtcttta gaaagaacta gctcgtctcc
    45721 accttcgata cgcgtatctt cttcacggtt gtagaccaaa tccacttttc cgccatagat
    45781 tgggtggtat ttgaagatat acttaccgta gagtgtttca cggttacgag tgtctgcaaa
    45841 catgtggtta agcgatacgg cgtttccaat tgttgcaaat gggtcgcgag tgaaatagag
    45901 gtttggcatc gggtcaattg caaatggata atctgattca actaagtcag ttagatcttt
    45961 agattcgtca ggaatttctg gcaattcaac tttttgaatc ccagccattg ttttttcaac
    46021 caattcttgg ttgtccttga tgccgtgaag caattcacga atagcaacct tggtttgacg
    46081 atcacggatg ttggcttcgt ctaagtattc ctcgataaat tgatcgcgga tttctggaga
    46141 ggtcaatgat tcagcagcga gttgttctag gtagagaacc tcaattcctt catcgcgaag
    46201 agcttgggca aatgcatcat gttctttttg agcatcttcc aagaaaggaa tatcatcaaa
    46261 aagaagcctt tcaagatagt ccggcaacaa gttttctaac tccttgcctg gacggtgcaa
    46321 cataactttt ttcagtttcc caatttctga gaagacctga attggatgtg aagacatgta
    46381 ttttcctcct taaaaatttt gacttggcac cattttgggt accgcttaca tgtatgttat
    46441 aacgcaattc ctaggtaatg tcaatggcta taatttctcg gaaaataata taaattatga
    46501 tgtttagcac aattttatgt tattatacat aacataggat agaatacata gatacaaatg
    46561 cataaacttc tataaaaagg atataaaatt tttttaaaaa atttttcaaa aaatatacaa
    46621 atgaccaaaa agtgtccaat tatcatctgg ttatcacttg gatctgtata aagagaaatg
    46681 tttgtgtgaa aggataaaaa gatgcaacat tcttttgaag atagattttg gagaagcatt
    46741 tcttgttcaa ttgtacaaat tatagaatct tttctgagta tttaggaaga gataattatc
    46801 ttattgaccc tctttttatt ttttgttata gtcgacgagg tgatagttta gaataatcac
    46861 ttattcacaa tctgtggaaa agtctggaga gtgaatggtc ttcacacaat gacctgaaag
    46921 aagcctgaga ataattatgg agagtagcat tctgagaggt gttagcagaa ccatatgaca
    46981 gagctgtttg aagagggaat attgaggagc aaaatcctga gcctaccagt tggagttgga
    47041 aagagctggc tgttagatca tggtttatta tccacaacct gtggataacc ttgtgaacaa
    47101 gagaagttgc taaagaagga gatatataac gatgaagaaa atcaaaccgc atggaccgtt
    47161 accaagtcag actcagctag cttatctggg agatgaacta gcagctttta tccacttcgg
    47221 tcctaatact ttttatgacc aagaatgggg gactggacag gaggatcctg agcgctttaa
    47281 cccgagtcag ttggatgcgc gtgagtgggt tcgtgtgctc aaggaaacgg gcttcaaaaa
    47341 gttgattttg gtggtcaagc accacgatgg ctttgtcctt tatccgacag ctcacacaga
    47401 ttattcggtt aaggtcagtc cttggaggag aggaaagggc gacttgctcc ttgaagtatc
    47461 ccaagctgcc acagagtttg atatggatat gggggtctac ctgtcaccgt gggatgccca
    47521 tagtcccctc tatcatgtgg accgagaagc ggactacaat gcctattatc tggctcagtt
    47581 gaaggaaatc ttatcaaatc ctaactatgg gaatgctggt aagttcgctg aggtttggat
    47641 ggatggtgcc agaggagagg gcgcgcaaaa ggttaattat gaatttgaaa aatggtttga
    47701 aaccattcgt gacctgcagg gcgattgctt gattttttca acagaaggca ccagtatccg
    47761 ctggattggc aatgaacgag ggtatgcagg tgatccactg tggcaaaagg tgaatcctga
    47821 taaactagga acagaagcag agctgaacta tcttcagcac ggggatccct cgggcacgat
    47881 tttttcaatc ggagaggcag atgtttccat ccgtccaggc tggttctacc atgaggatca
    47941 ggatcctaag tctctcgagg agttggtcga aatctacttt cactcagtag ggcgaggaac
    48001 tccactcttg cttaatattc cgccgaatca agctgggctc tttgatgcaa aggatattga
    48061 acgactttat gaatttgcga cctatcgcaa tgagctctat aaagaagatt tggctctggg
    48121 agctgaggta tctggtccag ctctttccgc agactttgct tgtcgccatt tgacagacgg
    48181 ccttgagacc agctcttggg caaacgatgc agacttgccc atccagttag aactcgactt
    48241 aggttctcct aaaacttttg atgtaattga gttgagagaa gatttgaagt taggccaacg
    48301 tatttctgct ttccatgtgc aagtagaggt ggatggtgtc tggcaggaat ttggcagggg
    48361 cttcacagtt ggtcataagc gcctcttgcg aggttcgcta gtagaggcac aaaaggtgcg
    48421 cgtgatgatt accgaggcac aatctattcc tgtattgacc aagatttccc tttataaaac
    48481 tcctggatta tcaaaaaaag aagttgttca ggaactagca tttgcagaaa aaagcctagc
    48541 tgtggcaaag ggagaaaatg cctattttac agttaagcgc agagaatgta gtggtccttt
    48601 agaagctaag atttcgattc aaccggggac aggtgtccat ggtgtcgcct atcaggacga
    48661 gatttatgtc cttcaattcc aagcagggga aagcaaaaaa gatttgcatc taccaaccct
    48721 gtatttcgca ggagataaaa ccttggattt ctatctgaac ctaacggtgg atggtcagct
    48781 tgtggatcaa cttcaagtcc aagtttcata aaagaagaac ctttgcgcga tgcaaaggtt
    48841 cttttggtta ttagtgactt ggtagccagc tgagggtgaa agttagttgt tcagctttta
    48901 agaggtcttg gtgttgaata gttgatacga gtgttttgtt cagtcggcat tctttgacaa
    48961 agttaaaatg gttgtggttt tgtttagtat ggatatccag ccatttatct tctttagcta
    49021 ggtagactcg tagatggtca aagagaggga ttccgaggtc atagcttggt tttcctggac
    49081 aggttggata aaatccgaga gctgaccaga tgtaccaagc agagagacta ccattgtctt
    49141 catctccagg ataggcttcc caacttgggt gaaaagcttt ctgacggagc gtcttgataa
    49201 gaagggcagt gtagtcaggg taatcgctgt agcggaagag ataagggatg tggaaactag
    49261 gctggttgga aatggctatt tgtccaaaag gagcagtagc catctcgctc atttcgtgaa
    49321 tttcataacc atagcctgtt gtttcaaaga ggggagcatc ttgacaggct ttcaaaagat
    49381 agttgctaaa ggtttctttt ccacccatca gttggattaa gccagggatg tcgtggagaa
    49441 cgcctaaagt agcttgaatg gcagagcatt cagcgtagtc tcgcccccaa ctataaggag
    49501 agaagtcagg gtgaaagttt ccttgattgt ctcgtgctcg catgtaacct gtctcagcgt
    49561 caaatagctg gcggtaattt tgtgaagcag ccttgtaggt ttcagcgatt tctatgttct
    49621 ctagtttttt ggcacagctg gcgatacaaa agtcactata ggcatagtct agagtatggc
    49681 taacactttc atggtggtcg gtagagaggt aacctagttc ttggtattgg gctagtccgt
    49741 ggcggccatt gatgccgaga gggtcggctt tgctggctgt ttcgagcatg gcttggaaga
    49801 gttctccttc taggtcgggg gccatgtcct tacaggcgct atctgcgata atgccatcta
    49861 gcagtgtacc tggcatcata ccccgttcat ctggagccag ccattttgga aggaaaccag
    49921 tatcgcggta gctattgagg aaaccttcta aaaagcgttg atagtgctcc ggtatgataa
    49981 gggcaaagag ggggaaggtg gtgcggaagg tatcccagaa accattgttg ctaaagagga
    50041 caccaggatt gacagtacca gtagccagat ccatgtggat ggctttccct gactcatcaa
    50101 cctcataaaa agtctgtggg aagaggaaga gtctgtagag acagtggtca aagaaggttc
    50161 ggtcagcctc tcctgtctct ataatgtcaa aacgatggag gagattttcc caatccgctt
    50221 gggcacttgt tttacagcta tcaaaatctt cttgaggtag attgattaga gcttgagaag
    50281 gagagatgaa agaagtggct agttgcatct cggtttgact acttgctaag tcaattcgcc
    50341 agtctccagc ttcttggctg atagcaagaa tatccgtgtt catttgcagg gcagtgaaca
    50401 tcgttagcga atttttgtta gtttcagttt taccttcttg tcgcagggca agagtccgct
    50461 tatctacttg ctctactgtc agttcatctg ctgcgtgaag atagagggag agggctttgc
    50521 cttgcttttg attcaaacga atagaagcac catagcaagt cggtgtgagc tgggtttcaa
    50581 tctgataacg cagagaaaag agcttcaaat agtgaggctg gaagcaagct ttatctatat
    50641 cataagaaga ctggcggtga aagaggctgt ctccccccag ttgactggtg acaggtgtca
    50701 gaaggagcca agagtagtcc ccaatccaag gactgggctg gtgagttaat cgaatcccct
    50761 gaaagatagg cagatgcgga tcaaaaaacc aagatccatc ctggtcactg gtctggggca
    50821 caaagtaatt cataccaaaa ggcacgcctg tgtatggcag ggtatttccc cgagagaagg
    50881 catgcttgct agcagtacca aagcgggtat cgatggtttc aagtagtggt ttcatagtct
    50941 ttcctttagc tgtttttcta cattatatca gaaaaagagg gcctttagaa aaggagtttc
    51001 aaaaataact attttgtaga aaaatgacta tcatttgtgt atgtattttc tgtctcaaaa
    51061 gctatatact gaacatgaaa cttgtttgaa agagggaatt gcacgatgat ttattcgaaa
    51121 gaaattgtta gagaatggct agatgaagta gcagagcggg ccaaggacca tccagagtgg
    51181 gtggatgttt tcgagcgttg ctacaccgat accttggaca atacggttga aatcttagaa
    51241 gatggttcaa cttttgtctt gactggggat attcctgcta tgtggcttcg ggattcgaca
    51301 gcccaactca gaccctacct gcatgtggct aaaagagatt ccctcctgcg tcagaccatt
    51361 gcaggtttgg tcaaacgtca gatgaccttg gtactcaagg atccctatgc taactccttc
    51421 aacattgagg agaactggaa agggcaccac gagactgacc acacagacct taacggctgg
    51481 atctgggagc gcaagtatga ggtggattcg ctttgctatc ctttgcagtt ggcttatctc
    51541 ctctggaaag agactggcga gactagtcag tttgatgaga tttttgtcgc agcgactaag
    51601 gaaattctcc atctgtggac ggtagaacaa gatcataaga actctcctta tcgttttgtc
    51661 cgagatacgg accgcaagga agacaccttg gtaaatgatg gctttggacc tgattttgca
    51721 gtgacaggaa tgacttggtc agcctttcgt ccgagcgatg actgctgtca gtatagttac
    51781 ttgattccgt caaatatgtt tgctgtagta gtcttgggtt atgtgcaaga aatcttcgca
    51841 gcattaaacc tagctgatag ccagagtgtt attgctgatg ccaagcgtct tcaggatgaa
    51901 atccaagaag gaatcgaaaa ctacgcttac accaccaaca gcaagggcga aaagatttac
    51961 gcttttgaag tggatggcct aggaaatgcc agcatcatgg atgatccaaa tgtaccaagt
    52021 ctactagctg cgccctatct gggctactgt tcggtcgatg atgaagtgta tcaagctact
    52081 cgtcgtacca ttttgagctc tgaaaatcca tatttctacc aaggagaata cgctagcggt
    52141 ctcggaagtt ctcatacctt ctatcgctat atctggccaa tcgccctttc tatccaagga
    52201 ttgacaacaa gagataaggc agagaaaaaa ttcttgctgg atcagctggt tgcctgcgat
    52261 ggtggtacag gtgtcatgca cgaaagcttc cacgtagatg atccgaccct ctactctcgt
    52321 gaatggttct cctgggctaa catgatgttc tgtgagttgg tcttggatta tttagatatt
    52381 cgctaagggg ctcgctttag ctcaaccgat tcttatcaga atcacaagtt tacatttaaa
    52441 acgttaaaat ttaaatttag aatgaggttt tacttcatgg aaaatgttgt tgtacatatt
    52501 atctcacata gccactggga ccgtgagtgg tacttgcctt ttgaaagcca tcgtatgcag
    52561 ttggtggaat tgtttgacaa tctctttgaa ctctttgaaa atgaccctga gttcaagagt
    52621 ttccacttgg atggacaaac tattgtcctt gacgactact tacaaattcg ccctgaaaat
    52681 cgcgacaagg tccaacgcta cattgacgag ggcaaactta aaattggtcc cttttacatc
    52741 ttgcaggatg actacttgat ctccagtgaa gccaatgtcc gcaatacctt gattggtcaa
    52801 caagaagctg ccaaatgggg taaatcaacc cagattggct actttccaga tacctttgga
    52861 aatatgggac aagcgcctca aattcttcaa aaatcaggca ttcacgtggc ggcctttggt
    52921 cgtggtgtga agccgattgg atttgacaac caagtccttg aagatgagca gtttacatcc
    52981 cagttttcag aaatgtactg gcagggtgtg gatggtagtc gtgttttagg tattctcttt
    53041 gctaactggt acagtaatgg gaatgaaatc ccagttgaca aagatgaggc cttgaccttc
    53101 tggaaacaaa aattgtcaga tgtgcgtgcc tacgcttcga ccaaccaatg gttgatgatg
    53161 aacggctgtg accaccagcc tgtacagaaa aatctgagcg aagccattcg tgtggcaaat
    53221 gaactcttcc cagatgtaat ctttgttcat agttcttttg atgaatatgt tcaagctgta
    53281 gaaggtgcgc ttcctgaaca cttatcaact gttacaggcg agttgaccag tcaggaaaca
    53341 gatggctggt acacacttgc caacacttct tcatcccgca tttacctaaa acaagccttc
    53401 caagaaaata gcaacctcct agagcaagtg gtagaaccct tgactattat cactggtgga
    53461 cacaaccaca aggaccagtt gacctatgct tggaaaacac ttttgcagaa tgcgccacat
    53521 gatagtatct gtggctgtag cgtggacgaa gttcaccgcg agatggaaac gcgttttgcc
    53581 aaggtcaacc aagtaggaaa ctttgttaaa agtaatttgc tcaacgagtg gaagggtaaa
    53641 attgctacgg ataaggctca aagtgactat ctctttactg tcattaacac aggcttgcat
    53701 gataaggtcg atactgtcag cacagtgatt gatgtggcga cttgtgattt caaggaattg
    53761 cacccaacag aaggctacaa aaagatggcc gctcttatct tgccaagtta ccgtgtggag
    53821 gacttggatg gtcgtcctgt agaggctaca atcgaagacc tcggagctaa ttttgaatac
    53881 gatttaccaa aagacaagtt ccgccaagct cgtattgctc gacaagtgcg cgtgaccatt
    53941 ccagttcagc tagcgccgct ttcttggaca accttccaat tgctggaagg aaaacaagaa
    54001 caccgtgagg gtatttacca aaacggagtg attgatacac cattcgtaac ggtgagtgtg
    54061 gatgacaaca tcacagtcta tgacaagaca actcacgaag cctatgaaga ctttatccgc
    54121 tttgaagacc gtggggacat cggaaacgag tatatctatt tccaaccaaa aggaacagag
    54181 ccaatctttg cagagcttaa gggccacgag gtcttggaaa acacagcttg ctatgctaaa
    54241 atcttgctca aacatgaatt gaccgtgcct gtcagcgcgg atgaaaagct agaagaagag
    54301 caacaaggta tcatcgagtt tatgaagcgt gaggctggac ggtcagaaga attgacaaac
    54361 attcctctgg aaactgagtt gactgtcttc gttgacaatc cacaaatccg cttcaagact
    54421 cgctttacca acactgccaa ggatcaccgt atccgtctct tggtcaagac tcataacacg
    54481 cgtccaagta atgattctga aagtatctat gaggtggtga cacgaccaaa caaaccagct
    54541 gcttcatggg aaaatcctga aaatcctcaa caccaacaag cttttgtcag tctgtatgac
    54601 gatgaaaaag gggtgactgt atccaataag ggattgaatg aatacgaaat ccttggggat
    54661 aacaccattg ccgtgaccat tttgcgtgca tcaggtgagc taggtgactg gggctacttc
    54721 ccaacgccag aagcacaatg cttgcgggag tttgaagtcg agtttgcact tgaatgccac
    54781 caagcccaag aacgcttctc agcctatcgt cgtgccaaag ccttgcagac atcgtttacc
    54841 agccttcagc ttgctagaca ggaaggaagc gtggttgcga ttggtagcct cttgagccat
    54901 tctgttctca gcataccgca agtttgtcca acagccttta aagtggcgga aaatgaagaa
    54961 ggctatgtcc tccgttacta taatatgagt caagaaaatg tgcgcatatc agaacatcaa
    55021 caaaccattc ttgacttact tgagcgacca tatccagttc attcaggact attggctcca
    55081 caagagattc gtacagaatt catcaaaaaa gaagaaattt aatttcaaaa agtaaacatc
    55141 aaaagaaagg aggggcgaaa aggtaagaac taactgctga tttgcccctt tttatggtaa
    55201 aaaaatgacc attgcaacga ttgatatcgg agggactggg attaagtttg ctagtctgac
    55261 tcctgatggg aaaatactag ataagacaag tatcccaaca ccaaaaaact tggaggactt
    55321 actagcttgg ctagatcaac gtttgtcaga acaggattat agtgggattg ctatgagcgt
    55381 tccaggtgcg gtcaatcaag agacaggtgt gattgatggc ttcagtgcgg tgccctacat
    55441 ccattgcttt tcttggtatg aggcgcttag ctcttatcag ctacctgtcc atttagaaaa
    55501 tgatgccaac tgcgttggac tcagtgaact actagctcat ccagagcttg aaaatgcagc
    55561 ctgtgtcgtg attgggacag ggattggcgg agccatgatt atcaatggta gacttcatcg
    55621 aggtcgccac ggtctgggtg gagaatttgg ctacatgaca acccttgccc ctgctggaaa
    55681 acttaataac tggtcgcaac tagcatcaac tgggaatatg gtacgatacg taattgaaaa
    55741 atctggtcat actgattggg acggtcgcaa gatttaccaa gaggccgcag ctggtaatgc
    55801 tctttgtcaa gaagccattg agcgcatgaa ctgcaatctg gcgcaaggct tgctcaatat
    55861 ccagtatctg atcgatccag atgtcatcag tctgggtggc tctatcagtc aaaatccaga
    55921 ttttatccaa ggtgttaaga aggctgttga agactttgta gatgcctacg aagaatacac
    55981 ggtcgcacca gttatccagg cctgcaccta tcacgcagat gccaatctct acggtgctct
    56041 tgtcaactgg ctacaggagg aaaagcaatg gtaagattta caggacttag tcccaaacaa
    56101 acgcaagcta ttgaggtttt aaaaggtcac atttctctac cagatgtgga agtggctgtc
    56161 actcagtctg accaagcctc tatctctatc cagggtgagg aaggtcacta tcaattgacc
    56221 taccgcaaac ctcaccaact ttatcgtgcc ttatccttgt tggtaacagt tctagcagaa
    56281 gctgataaag tagagattga ggaacaagca gcttacgaag atttggctta catggttgac
    56341 tgttctcgaa atgcggtgct gaatgtggct tctgccaagc agatgattga ggtcttggct
    56401 ctcatgggct actcaacctt tgagctttac atggaagaca cttaccagat tgaaggacag
    56461 ccttactttg gctatttccg tggagcttat tcagcagagg agttgcagga aatagaagcc
    56521 tatgctcaac agtttgacat gacctttgta ccatgcatcc agaccttggc ccacttgtcg
    56581 gcctttgtca aatggggtgt caaggaagtg caggagctcc gtgatgtaga ggacattctt
    56641 ctcattggcg aagaaaaggt ttatgacctg attgatggca tgtttgccac gttgtctaaa
    56701 ctgaagactc gcaaggtcaa tatcgggatg gacgaagccc acttggttgg tttgggacgc
    56761 tacctgattt tgaacggtgt tgtggatcgt agtctcctca tgtgccaaca cttggagcgc
    56821 gtgctggata ttgctgacaa atatggtttc cactgccaga tgtggagtga tatgttcttc
    56881 aaactcatgt cagcggatgg ccagtacgac cgtgatgtgg aaattccaga ggaaactcgt
    56941 gtctacctag accgtctcaa agaccgtgtg actctggttt actgggatta ttatcaggat
    57001 agcgaggaaa aatacaaccg taatttccgc aatcatcaca agattagcca tgaccttgca
    57061 tttgcagggg gagcttggaa gtggattggc tttacacctc acaaccattt tagccgtcta
    57121 gtggctatcg aggctaataa agcctgccgt gccaatcaga ttaaagaagt catcgtaacg
    57181 ggttggggag acaatggtgg tgaaactgcc cagttctcta tcctaccaag cttgcaaatc
    57241 tgggcagaac tcagctatcg caatgaccta gatggtttgt ctgcgcactt caagaccaat
    57301 actggtctaa cggttgagga ttttatgcag attgaccttg ccaacctctt accagaccta
    57361 ccaggcaatc tcagcggtat caatcccaac cgctatgttt tttatcagga tattctttgt
    57421 ccgattcttg atcaacacat gacacctgaa caggacaaac cgcacttcgc tcaggctgct
    57481 gagacgcttg ctaacattaa agaaaaagct ggaaactatg cctatctctt tgaaactcag
    57541 gcccagttga atgctatttt aagtagcaaa gtagatgtgg gacgacgcat tcgtcaggcc
    57601 taccaagcgg atgataaaga aagtttacaa caaatcgcca gacaagaatt accagaactt
    57661 agaagccaaa ttgaagactt ccatgccctc tttagtcacc aatggctgaa agaaaacaag
    57721 gtctttggtt tggatacagt tgacatccgt atgggcggac tcttgcaacg catcaaacga
    57781 gcagaaagcc gtatcgaggt ttatctggct ggtcagcttg accgcatcga cgagctggaa
    57841 gttgaaatcc taccatttac tgacttctac gcagacaagg atttcgcagc aactacagcc
    57901 aaccagtggc ataccattgc gacagcgtcg acgatttata cgacttaata ctcttcgaaa
    57961 atctcttcaa accacgtcag cttccatcta caacctcaaa acagtgtttt gagcagcctg
    58021 cagctagctt cctagtttgc tctttgattt tcattgagta taaaaacaag aacaccttgc
    58081 ttggcgcagg gtgtttcgcg tgaaacagaa gaattatctg gtttcaaatg ctacagttag
    58141 acaaacttat gataaaatag cagaaagtga atgtttccta agagcaattc tatcgatgag
    58201 caattggagg tattatgcta cacttaaaat tagtaaaaca agaaatagaa gctgaaaagc
    58261 cagcatctgt agaagcttgg atcatttccg tcaaatttaa aaaaggttgc taccgacata
    58321 tatagattcc aaaaacaaaa acgttagcgg aactagcaga tgtgatttta tggagttttg
    58381 attttgcaaa tgatcatgct cacgcatttt tcatggataa tgttgagtgg agtcatgcag
    58441 attcttactt tcttagcttt gttagtgacg atgttgaaga acgttacaca gaaaatgtct
    58501 atctggatag cctaagtgtc aaacaaaaat ttaagtttat tttcgacttc ggtgatgaat
    58561 ggcgttttga atgccaagtg ctgagagaaa tcgagacaga ggacgaagaa gcttatctcg
    58621 tacgttcggt tggaacgtcg ccagaacaat atccagatta tgatggtttt gactatgaag
    58681 aatggtaaaa ttgaaatcta aaaaacaatt ggaatcagtc tgtgtaggct tagtat
//
LOCUS       DAVKGU010000004        53099 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKGU010000004 DAVKGU010000000
VERSION     DAVKGU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.004"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            5..92
                     /locus_tag="VW077_000205"
     tRNA            5..92
                     /locus_tag="VW077_000205"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Ser,seq:gct)
     gene            complement(218..1153)
                     /locus_tag="VW077_000206"
     CDS             complement(218..1153)
                     /locus_tag="VW077_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9454302.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1150..2010)
                     /locus_tag="VW077_000207"
     CDS             complement(1150..2010)
                     /locus_tag="VW077_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9454303.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2118..4280
                     /locus_tag="VW077_000208"
     CDS             2118..4280
                     /locus_tag="VW077_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9454304.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENQNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHTQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRRHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4372..5631
                     /locus_tag="VW077_000209"
     CDS             4372..5631
                     /locus_tag="VW077_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9454305.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5645..6511
                     /locus_tag="VW077_000210"
     CDS             5645..6511
                     /locus_tag="VW077_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9454306.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6525..7358
                     /locus_tag="VW077_000211"
     CDS             6525..7358
                     /locus_tag="VW077_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9454307.1"
                     /translation="MKQDERKALIGKYILLILGTVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7359..7439
                     /locus_tag="VW077_000212"
                     /pseudo
     CDS             7359..7439
                     /locus_tag="VW077_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7716..9158
                     /gene="gtfA"
                     /locus_tag="VW077_000213"
     CDS             7716..9158
                     /gene="gtfA"
                     /locus_tag="VW077_000213"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9454308.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFRDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            9296..10489
                     /locus_tag="VW077_000214"
     CDS             9296..10489
                     /locus_tag="VW077_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9454309.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            11176..13155
                     /locus_tag="VW077_000215"
     CDS             11176..13155
                     /locus_tag="VW077_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9454310.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNVDKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13222..14718
                     /locus_tag="VW077_000216"
     CDS             13222..14718
                     /locus_tag="VW077_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454311.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14718..15644
                     /locus_tag="VW077_000217"
     CDS             14718..15644
                     /locus_tag="VW077_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454312.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15653..16720
                     /locus_tag="VW077_000218"
     CDS             15653..16720
                     /locus_tag="VW077_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454313.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16731..17657
                     /locus_tag="VW077_000219"
     CDS             16731..17657
                     /locus_tag="VW077_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454314.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQTELARYQKGLN"
     gene            18276..19085
                     /locus_tag="VW077_000220"
                     /pseudo
     CDS             18276..19085
                     /locus_tag="VW077_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            19097..19974
                     /locus_tag="VW077_000221"
                     /pseudo
     CDS             19097..19974
                     /locus_tag="VW077_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            19987..20790
                     /locus_tag="VW077_000222"
     CDS             19987..20790
                     /locus_tag="VW077_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9454315.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGRN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(20823..21533)
                     /gene="treR"
                     /locus_tag="VW077_000223"
     CDS             complement(20823..21533)
                     /gene="treR"
                     /locus_tag="VW077_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9454316.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21718..23685
                     /gene="treP"
                     /locus_tag="VW077_000224"
     CDS             21718..23685
                     /gene="treP"
                     /locus_tag="VW077_000224"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9454317.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QCVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23863..25488
                     /gene="treC"
                     /locus_tag="VW077_000225"
     CDS             23863..25488
                     /gene="treC"
                     /locus_tag="VW077_000225"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9454318.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25630..25878
                     /locus_tag="VW077_000226"
     CDS             25630..25878
                     /locus_tag="VW077_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9454319.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26085..26879
                     /gene="racE"
                     /locus_tag="VW077_000227"
     CDS             26085..26879
                     /gene="racE"
                     /locus_tag="VW077_000227"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9454320.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            26876..27847
                     /locus_tag="VW077_000228"
     CDS             26876..27847
                     /locus_tag="VW077_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9454321.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27823..28344
                     /locus_tag="VW077_000229"
     CDS             27823..28344
                     /locus_tag="VW077_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9454322.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28341..28802
                     /locus_tag="VW077_000230"
     CDS             28341..28802
                     /locus_tag="VW077_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9454323.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28793..29533
                     /gene="xerD"
                     /locus_tag="VW077_000231"
     CDS             28793..29533
                     /gene="xerD"
                     /locus_tag="VW077_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9454324.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29533..30261
                     /locus_tag="VW077_000232"
     CDS             29533..30261
                     /locus_tag="VW077_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9454325.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30280..30849
                     /locus_tag="VW077_000233"
     CDS             30280..30849
                     /locus_tag="VW077_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9454326.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            30836..31558
                     /locus_tag="VW077_000234"
     CDS             30836..31558
                     /locus_tag="VW077_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9454327.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31558..31800
                     /gene="yidD"
                     /locus_tag="VW077_000235"
     CDS             31558..31800
                     /gene="yidD"
                     /locus_tag="VW077_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9454328.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(32025..32990)
                     /locus_tag="VW077_000236"
     CDS             complement(32025..32990)
                     /locus_tag="VW077_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9454329.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDKVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQKVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(33086..33838)
                     /locus_tag="VW077_000237"
     CDS             complement(33086..33838)
                     /locus_tag="VW077_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9454330.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(33835..34791)
                     /locus_tag="VW077_000238"
     CDS             complement(33835..34791)
                     /locus_tag="VW077_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9454331.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(34781..35740)
                     /locus_tag="VW077_000239"
     CDS             complement(34781..35740)
                     /locus_tag="VW077_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454332.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSILFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(36163..36864)
                     /gene="galE"
                     /locus_tag="VW077_000240"
     CDS             complement(36163..36864)
                     /gene="galE"
                     /locus_tag="VW077_000240"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9454333.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            37265..38329
                     /gene="pepA"
                     /locus_tag="VW077_000241"
     CDS             37265..38329
                     /gene="pepA"
                     /locus_tag="VW077_000241"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9454334.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSDLAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            38351..38647
                     /locus_tag="VW077_000242"
     CDS             38351..38647
                     /locus_tag="VW077_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9454335.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            39329..39772
                     /locus_tag="VW077_000243"
     CDS             39329..39772
                     /locus_tag="VW077_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9454336.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            39776..40540
                     /locus_tag="VW077_000244"
     CDS             39776..40540
                     /locus_tag="VW077_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9454337.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            40555..41283
                     /locus_tag="VW077_000245"
     CDS             40555..41283
                     /locus_tag="VW077_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454338.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            41276..42796
                     /locus_tag="VW077_000246"
     CDS             41276..42796
                     /locus_tag="VW077_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9454339.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            43141..43947
                     /locus_tag="VW077_000247"
     CDS             43141..43947
                     /locus_tag="VW077_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9454340.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(44113..44649)
                     /locus_tag="VW077_000248"
     CDS             complement(44113..44649)
                     /locus_tag="VW077_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9454341.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTGQEVVQFYLDLLIPKN"
     gene            44782..45672
                     /locus_tag="VW077_000249"
     CDS             44782..45672
                     /locus_tag="VW077_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9454342.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            45916..46269
                     /locus_tag="VW077_000250"
     CDS             45916..46269
                     /locus_tag="VW077_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9454343.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            46270..47307
                     /locus_tag="VW077_000251"
     CDS             46270..47307
                     /locus_tag="VW077_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9454344.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYSEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMALESGATAVV
                     AERGQEGIAKVREILGGGADAALECVGTEAAIDQALGVLHNGGRMGFVGVPHYENRAI
                     GSTFGQNTIIGGGPASVTTYDKQVLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(47353..48360)
                     /gene="galR"
                     /locus_tag="VW077_000252"
     CDS             complement(47353..48360)
                     /gene="galR"
                     /locus_tag="VW077_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9454345.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            48524..49702
                     /locus_tag="VW077_000253"
     CDS             48524..49702
                     /locus_tag="VW077_000253"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9454346.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            49721..51202
                     /locus_tag="VW077_000254"
     CDS             49721..51202
                     /locus_tag="VW077_000254"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9454347.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKAQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNYPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            51272..51676
                     /locus_tag="VW077_000255"
     CDS             51272..51676
                     /locus_tag="VW077_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9454348.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERQVRI"
     gene            complement(51722..52987)
                     /gene="dcm"
                     /locus_tag="VW077_000256"
     CDS             complement(51722..52987)
                     /gene="dcm"
                     /locus_tag="VW077_000256"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU9454349.1"
                     /translation="MKVLELFAGVGGFRIGLENADKQLFKTKWANQWEPSRKSQDAFE
                     VYDYHFPNSKNINISISDITDEQFSKMDADMIVGGFPCQDYSVARSKKNEKGIEGKKG
                     VLFWEIIRATEIIKPKYLILENVDRLLKAPSKQRGRDFAIMLTAFNNLGYSVEWRVIN
                     AAEYGRSQRRRRVFFFVYRNDTVFAQKIDNLYEKNEEIFEDNRYDDYIFNQGLFAKQF
                     PIKPIAVKNRHVFYELPNDIVEVSDTFTGTVWNTGIMRRGKYYSIDTEPNYNGNPITL
                     GEILQDESEVPEKYFLTDQSKLEKFQYLRGPKKIERTSSDGHQYIYSEGGMSPYDDLN
                     LPGRTMLTSEGTVNRSTHLLFVNNKYRLITPIEAERLQDFPDDWTAKKKLSDDSIVEV
                     SDKMRMFFMGNALVTEIVKEIAKFIKEID"
BASE COUNT        15360 a         9782 c        11953 g        16004 t
ORIGIN      
        1 aattggagaa ttactcaaga ggctgaagag gacggtttgc taaatcgtta ggtcgggtaa
       61 ctggcgcaag ggttcgaatc ccttattctc cgttttaatg agagtttgta actctctttt
      121 ttatttttag agccaataaa acttcagatc cgtaaaactt aattgaatta ctaatctgaa
      181 gttattttta tatcgtttat attataattg tgttatttta cttccaacta ttgagagaaa
      241 tttcgccact atttagccag atttcttttc cgttatcaca ttgaactaaa agttttccat
      301 tttctgagat gtctttagca agtcccttgt agtctttttg ctctagtgtg aaagtgactt
      361 cttttcctag aatgaatgac tgttttttgt ataggtataa tagctcttct gctggtgttt
      421 cgaagaaagc acgccagatt tctatgatca attcattcct tgttatagga gctgtagctt
      481 taaataagct ggcagctttt tcttttaatt cctgagggaa gtctttaata gtgaaattga
      541 tacctactcc aataatgata tctgtgacta agccagtttc tacagaggtc attgcttcag
      601 taaggattcc tccaatttta tgattgttta gatagatatc attgacccat tttatgtcga
      661 catctattaa agttaggttc ttaatggctt tgtagacagc tccagctaca agtagtgtgt
      721 aggatggtaa tttgtcatag gggagatttg gtttaagatg gagtgtcata taaataccac
      781 cttgtggtga gtagaaggaa cgttgaaaac ggcctcggcc tgctgtttga taggaagcta
      841 gatagagggt atttgcttca tggcctaaat caattgcttc ttttgcatct agttgtgttg
      901 attttgtttc gggtttaaag ctgactttaa ttggaagatt ttcttctaga atctctggaa
      961 gaataaggtc accattcatc agtttatatc ctctattttt gatactatca atttcaatgc
     1021 cttcttgttc tagtcgcttg atggctttcc aaattgctgt tcggcttagg gatagttttt
     1081 ctgcgatttt ttctccgctg atatagtcgg tttctttaga taggatttgg tagacagctt
     1141 ggtaggattt cataatgttg cctttcttac tagttggtat tgagagtatt cttttcttgt
     1201 atgacttgga gtctgattaa agtattgttt ataagctttc gaaaaatgga gtggatctga
     1261 aaaacctacc gagtatgcaa ttaccttgat ggactcttgg gtattttcga gaagttgtct
     1321 agctcggtgc attcgaacgt agagtaggta ttctttgggt gataaggtat taaattcttt
     1381 gaatacgctt gataagtagc ttctgtgaac ggatagttct tttgctaaat cttgaattgt
     1441 aagtgattga ggatagtggc tatcaattaa tcgtttgcat tcaagataga gttggtgggt
     1501 tgatgaaata ttcttttttt tctgattggg agcaatagtt cccagatgaa acatcagttc
     1561 atgaagttgt cccatgatat ggagttgagc taattcactt gattttgtaa tctgagcgaa
     1621 gcggacaatg tctgagatga gttttgcagt agtctgggta tgacaagttt cagattggat
     1681 gagataggat tgatcagaaa tttgagaaag agcaaaataa tcaggggatt tccctccagt
     1741 gattcctaac cagtagtagg cccaaggttc tttactatct gcttgataaa aggttagttc
     1801 ctctggtttt aatagaaaga aatctccttc ttttaaatca acaattttac ccttgtaata
     1861 aaattttcct tgtcctttag taatgtaatg tagaacgtat gtatcacgaa tggctggacc
     1921 aaaagagtaa ttaggtgtgc attcctcata tccataaaag cttagggcaa ggtcgattgt
     1981 tccagtctgg tattctgaaa aaactaacat gtgctacctc ccacctaaca ttttaccata
     2041 ttcttgagac atttttctat tttggaaagc gatttcaggt gataaaatat aatcaataaa
     2101 gtgatgaggt gaagtaaatg ggagttagga tagagaataa tctattttat gttgagagta
     2161 aaaatctaag tttgattatt gaaaatcaaa atggctactt acttttgaaa catttaggaa
     2221 agactattaa gaactataaa ggttccaata gtgtttatga acgagaccat gccttttcag
     2281 gaaatccaac ggctactaat cgaaccttta gtttagatac tcagcgacag atttttggac
     2341 aacatggctt aggagatttt aggaaaccaa ccatacaggt tcagcatagt gtaactgaag
     2401 taacagactt tcgatttgta gaagcaaaga ttttaaaagg tcagaatggt ccacagggct
     2461 taccttctcc acatagcatg gacgatacag agactcttgt cttaatgtta gaagattcta
     2521 aggctcaact tagtctgact ttgtattata ctacttttaa taatgatgcg actattgcta
     2581 gctacagtaa attagataat aatagtaatc aggaagttgt catccataaa gatttttctt
     2641 ttatggctga ttttccagct gcagattacg aaatagtaac tctgcagggt gcttatgctc
     2701 gtgaaaagac tgttagacgt caacaggtag aacaaggaat cttttcgatt agctcaaacc
     2761 gaggtgcttc tggtcatact caaacaccag ctcttctact atgcgaacaa ggagtcacag
     2821 aggatgctgg gaatgtgttt gctattcaac taatgtatag tggcaacttt gaagcttttg
     2881 ttcaaaaaaa tcaattgaat gaagttcggg tggctattgg cattaatcca gaaaactttt
     2941 cttggaagtt agcccctgag gaatactttg aaacaccggt agctttagtg actcattcag
     3001 atcagggatt aactggtatt agtcatgaaa gtcagaattt tgtactgaag cacattatgc
     3061 taagtgaatt ttctaaaaaa gaacgtccaa ttctaatcaa taactgggaa gctacttact
     3121 ttgactttca gagagaaaaa ctgttagagt tagcagatga agctaagaaa gttggcattg
     3181 aactttttgt attagatgat ggttggtttg gcaatcgttt tgatgataat cgtgctttag
     3241 gtgattgggt tgttaatgag gaaaaactgg gtggaagtct agaaagtctg atttcagcta
     3301 tccatgaaag aggtttgcag tttggacttt ggttagaacc cgaaatgatt tctgtagata
     3361 gtgatttgta tcgtcgacat cctgactggg ctattcaggt tcctgactat gagcatactt
     3421 attctcggaa tcaattagta cttaatcttg ccaatcctca ggtagtagaa tacttgaaaa
     3481 gtgtcttaga tcaactccta tcttatcatg atattgatta cattaaatgg gatatgaacc
     3541 gcaatatcac taagctaggg aatggattaa cttatctaga gacacagatg caatctcatc
     3601 agtacatgct ggggctttac gaactcgttt cttatctgac agagaagcac agccatattc
     3661 tctttgagtc ctgctctggt ggtggtggac gaaatgatct tggtatgatg cgctatttcc
     3721 cacaagtctg ggctagtgat aatactgatg ccattgcacg tttaccaatt caatacggtt
     3781 catcctatct ctatccaacc atttctatgg gggctcatgt gtcagcagta ccaaatcatc
     3841 agatgggacg aatgacacca ttagaaacac gtggccttgt agcaatgatg ggaaatttgg
     3901 gctatgaact tgatttgaca aatttatcag atgaagagaa agctacgatt gctaatcagg
     3961 tgaacttgta taaagaatta cgaccagtag ttcagttagg acagcagtat agactaatta
     4021 atcctgatac tgcatccaat gaagcagctg tacaatttaa ttacggaaat caaacgattg
     4081 taacctacgt tcgcgtttta tctgttgtag agaccatgga aacaacttta aaattaaaag
     4141 atttggatga agagggacta tataaattac aggaaaatgg cgaagtttac tcaggtgcag
     4201 aactaatgta tgcgggctta actgttatct tatcccaagg agattttttg agtagacagt
     4261 atatttttag aaaactatga tatgtactga cctcaaaagt tagatttatt ctatctaact
     4321 tttgaggtgc agtttgataa gggtgtataa atttataaag gaggctttct aatggaatgg
     4381 tataaaaaaa tcggacttct tgcaactaca ggtttagctt tgtttgggct cggtgcttgc
     4441 tccaactatg gtaaatctgc ggatgacaca gtgaccatcg agtatttcaa ccagaaaaaa
     4501 gaaatgacca aaaccttgga agaaatcact cgtgattttg agaaggaaaa ccctaagata
     4561 aaggtcaaag tcgtcaatgt accaaatgct ggtgaagtat tgaagacacg cgttctcgca
     4621 ggagatgtgc ctgatgtggt caatatttac ccacagtcca tcgaactgca agaatgggca
     4681 aaagcaggtg tctttgaaga tttgagtaac aaagactacc tgaaacgcgt gaaaaatggc
     4741 tacgctgaaa aatatgctgt aaacgaaaaa gtttacaacg ttccttttac agctaatgct
     4801 tatggaattt actacaacaa agataaattc gaagaactgg gcttgaaagt tcctgaaacc
     4861 tgggatgaat ttgaacagtt agtcaaagat atcgttgcta aaggacaaac accatttgga
     4921 attgcaggtg cagatgcttg gacactcaat ggttacaatc aattagcctt tgcgacagca
     4981 acaggtggag gaaaagaagc aaatcaatac cttcgttatt ctcaaccaaa tgccattaaa
     5041 ttgtcggatc cgattatgaa agatgacatc aaggtcatgg acatccttcg catcaatggc
     5101 tctaagcaaa agaactggga aggtgctggc tataccgatg ttatcggagc cttcgcacgt
     5161 ggggatgtcc tcatgacacc aaatgggtct tgggcgatca cagcgattaa tgaacaaaaa
     5221 ccgaacttta agattgggac cttcatgatt ccaggaaaag aaaaaggaca aagcttaacc
     5281 gttggtgcgg gagacttggc atggtctatc tcagccacca ccaaacatcc aaaagaagcc
     5341 aatgcctttg tggaatatat gacccgtcca gaagtcatgc aaaaatacta cgatgtggac
     5401 ggatctccaa cagcgatcga aggggtcaaa caagcaggag aagattcacc gcttgctggt
     5461 atgaccgaat atgcctttac ggatcgtcac ttggtctggt tgcaacaata ctggaccagt
     5521 gaagcagact tccatacctt gaccatgaac tatgtcttga ccggtgataa acaaggcatg
     5581 gtcaatgatt tgaatgcctt ctttaacccg atgaaagcgg atgtggatta ggagtagaga
     5641 gactatgaaa aaagtattac aaaaatattg ggcatgggct tttgtggtca tccccctctt
     5701 gttacaagca attttcttct atgtgccgat gtttcaagga gccttttaca gttttaccaa
     5761 ctggacagga ttgacttata actacaaatt tgtcggctta aacaacttta agctcctctt
     5821 catggatcca aaattcatga atgcgattgg ctttaccgca atcattacga ttgccatggt
     5881 ggttggtgag attgcactcg ggatcttcat tgcgcgtgtc ttgaattcta aaatcaaagg
     5941 ccaaaccttc ttccgtgctt ggttcttctt cccagctgtt ttatctggtt tgacagtggc
     6001 tttgatcttc aagcaagtct tcaactacgg tcttccagcg attgggaatg cccttcatat
     6061 tgaatttctc caaaccagtc ttttagggac taagtgggga gcaatctttg cggctgtctt
     6121 tgtccttctt tggcaagggg tggctatgcc catcatcatc ttcctagctg gtttgcaatc
     6181 tattccaact gagattacag aggcagcaag gattgatggt gcgactagca agcaagtttt
     6241 ctggaacatt gaattgcctt acttgctacc aagtgtctct atagtcttta tcctagccct
     6301 aaaaggtggg ctgactgcct ttgaccaagt ctttgccatg accggtggtg gtccaaacaa
     6361 tgccacaacc tcacttgggc tcttggttta taactatgcc tttaaaaaca accaattcgg
     6421 ttatgccaat gccattgccg taatcttgtt cttcttaatt gtagtgattt cgatcatcca
     6481 attgagagta tctaagaaat ttgaaattta agaggagaag catgatgaaa caagatgaaa
     6541 gaaaagccct gattggcaaa tacattctat tgattctagg aacggttctg attttagtgc
     6601 cgctccttgc taccctcttt agttccttca aacccactaa ggatattgta gataatttct
     6661 ttggttttcc aaccaacttc acatgggaca actttagccg tctcttagct gatgggattg
     6721 gaggctatta ttggaactct gtcgtcatca ctgtcttgtc tttacttgca gtaatgatct
     6781 ttatccctat ggcagcctac tccatcgctc gcaatatgag taaaagaaaa gcctttacca
     6841 tcatgtatac cctcttaatc ctcggaatct tcgtaccttt ccaagtcatc atgattccga
     6901 ttacggttat gatgagtaaa ctcggtttgg ctaatacctt tggtttgatc ttgctctact
     6961 tgacctatgc gattccacag accctctttc tctatgttgg ctatatcaaa atctcgattc
     7021 cagaaagtct ggatgaagca gcagagatcg atggggctaa tcaatttaca acctatttcc
     7081 gcatcatctt cccaatgatg aaaccgatgc atgcgacaac catgatcatc aatgcccttt
     7141 ggttctggaa tgacttcatg ttgccactcc ttgtcttgaa ccgggattcc aaaatgtgga
     7201 ctctgccttt gttccaatac aactacgcag gccaatattt caacgactac ggaccaagct
     7261 ttgcctctta cgtggtcggc attatcagta tcaccattgt ctatctcttc ttccaacgcc
     7321 atatcatttc aggaatgagc aacggggcag tgaagtaata ctcaatgaaa atcaaagagc
     7381 aaactaggaa gctagccgca ggttgctcaa aacactgttt tgaggttgtg gatgaaactg
     7441 acgaagtcag taaccatacc tacggtaagg caacgctgac gtggtttgaa gagattttcg
     7501 aatagtataa gaagagctca tggaatctat agtctttgac acagaggaag agttcagctt
     7561 gttgataatt ctatgtagaa aacaagaaag tagtactttg tttatcacag tctatattag
     7621 tacgagtttg atgataaata gagatttaaa atgaatttta tttatgaagc cagatcatat
     7681 gagacttggc ttcaattaga aaaaggagag taatgatgcc aattcaaaat aaaaccatgt
     7741 tgattaccta ttctgatagc cttggaaata atcttaaaga cttatatgat aatttggaag
     7801 agcattttcg agatgctatt ggaggagttc accttttacc atttttccca tcaacaggtg
     7861 atcgtggatt tgcgccagtt gactacgacg aagtggactc agcttttggt gattgggagg
     7921 atgttaagcg tttaggtgag agatattatc ttatgtttga ttttatgatt aatcatattt
     7981 ctcgtcaatc caagtattat aaggactatc aagaaaaaca tgaagccagt gaatttaaag
     8041 ctctcttttt aaactgggat aagttttggc cagaaaaccg tccgacacag tctgatgtag
     8101 atttaattta caagcgtaag gatcgtgcac caaagcaaga gattgtgttt gaagatggtt
     8161 cagtggaaca tttgtggaat acctttggtg aagagcagat tgatcttgat gtgaccaaag
     8221 aagtaactat ggaatttatc cgtaagacca ttcagcactt ggcaagtaat gggtgtgatt
     8281 tgattcgtct agatgccttt gcttatgcag tgaagaaatt ggatactaat gatttctttg
     8341 tggaaccaga tatttgggat ttattggaca aagttcgaga tatcgctgct gagtatggga
     8401 cagagctttt acctgagatt catgaacact attcgattca gtttaaaata gcagaccatg
     8461 attactatgt ttatgatttt gctcttccaa tggtgacact ttatactctt tacagttcca
     8521 gaacagagcg tttggctaag tggttaaaga tgagcccgat gaagcaattt acgacgctag
     8581 atacccatga tgggattgga gtagtagatg tcaaggatat cctgaccgat gaggagattg
     8641 actatgcttc aaatgaactc tataaggttg gagccaatgt caaacgtaag tactctagtg
     8701 ccgagtataa caacttagat atctaccaaa tcaattcaac ctactattca gcgcttggag
     8761 atgatgatgt caagtatttt ctcgctcgtc taattcaagc ttttgcccca ggtattcctc
     8821 aggtttacta tgtgggtcta ttagcaggca agaatgactt gaaattatta gaagaaacta
     8881 aagaaggtcg aaatattaat cgtcattact atagcaacga ggaaatagca aaagaagtgc
     8941 aacgacctgt tgtgaaggcc cttctcaatc tattttcttt ccgtaaccgt tcagaagcct
     9001 ttgatctaga agggactact gagatagaga caccaacagc tcacagcatt gtaatcaaac
     9061 gtcaaaataa agataagtcc gtaacagcag tagtagaaat tgatttgcaa aatcagactt
     9121 atcgggtaat tgagaatgga gttgaagtat cattttgaag ccttgatatg gtggataaaa
     9181 tagggttttt attttggaaa acgtttcctt tgttttcaaa ttgctaaaaa agtggtacaa
     9241 tataggatag cttactatta tctgaatcag ctgatttgga gagaaaggat tcattttgaa
     9301 atcaataggc tttattgaaa agctgaaggg gttgtctagt aaagagctga ttttattggg
     9361 aattatccta agtatctttt taccctttta tctttttgta gttgtactct gtttatatat
     9421 tatcagtttg atttttacag gagacatgaa aagtattctt cagaaaatgg gggagcatcc
     9481 gatgctgctt ctttttctta gctatagtac tgttatatcc attcttgcac aaaattggat
     9541 gggtcttgtg gcttcagtag gaatgtttct atttactatt ttctttttgc actatcagtc
     9601 gattttatcc cataaattct ttcgattgat tttgcagttc gtcttgtttg gtagtgtctt
     9661 gtcagctgct tttgccagtt tagaacattt ccaaattgtg aagaaattta actatgcttt
     9721 tctttcaccc aatatgcagg tgtggcatca gaaccgggca gaagtgacct tctttaatcc
     9781 taattattat ggaattattt gttgtttctg tattatgatt gctttctatc tgtttacaac
     9841 gaccaagttg aattggttga aagtattctg tgtgattgca ggctttgtta atctctttgg
     9901 tttgaacttt actcaaaatc gaactgcctt tcctgctatt atcgctggag caattatcta
     9961 tctctttacg actattaaaa actggaaggc cttttggctt agtattgggg tcttcgcgat
    10021 tggtttgagt ttcctctttt ctagtgattt gggagttcga atgggtactt tagactcttc
    10081 tatggaagaa cgcatttcta tctgggatgc tgggatggcc ttgtttaagc aaaatccttt
    10141 ttggggtgaa gggccattga cctatatgca ctcttatcct cggatacatg ctccttatca
    10201 tgaacatgcc cacagtcttt atattgatac gattctgagt tacggaattg tgggtaccat
    10261 tttattagtt ttgtcttctg ttgctcctgt tcgcttgatg atggatatga gtcaggagtc
    10321 ggggaaacgt ccgattatcg gcctttatct atctttcctt acagtggttg ctgtgcacgg
    10381 aatttttgac ttggctctct tctggattca gtcaggcttt attttcttgc tagttatgtg
    10441 cagtattcca ttggagcatc gaatgttggt atcggacatg acggattaag ttttataagt
    10501 ttgaaatctt ctaccttggg taggagattt ttttatacga tgggcatgtc gtgcatctga
    10561 ggtgtaagtc ctctgtaggg atccactacc ggtgaataca atagcgctcc ccaagcctga
    10621 ctatcgtgag gtagcggatt aaaatggtct ggggatagat cgttttaagt ctgacgcagg
    10681 aaataagaat tgtcagagaa agcaatagtg aaatcgtagg ctatgaatag aaacgtgata
    10741 gcgaggagca tatagtggat aaggtagcga gaaattttaa agtataaaag atagtcttaa
    10801 tctatatatg taaatcacaa aagtaattga attcggaata aggttggtgt gaaaagacat
    10861 agtaaactga atcttgaata ctagaatgat acttctacaa tattgttata atttaatttg
    10921 aattccaaaa tcacattgtt ccgtccgtat ttcattttac tgtatgtctt cctaaactcc
    10981 aaagtttctg cgtcaaattt cctattttca ctgtagtctt tgacacttcg aacgacaatt
    11041 tgaatcatat attcaaaaaa aaatgaaaaa tttgtgccaa caagcctaaa tattctgaaa
    11101 atttgtttga tttttaatgg ataatgtgat ataatggttg tcaatctata attcacataa
    11161 acagaggagt agaaaatgaa aaaaaatcgt gtatttgcta cagcaggtct tgttttatta
    11221 gcagcaggtg tacttgcagc atgcagttct tcaaaatcat ctgattcatc agcccctaaa
    11281 gcttatggct atgtttatac agcagaccca gaaaccttgg actacctgat ttcaagtaaa
    11341 aatagtacaa cagtagtgac ttcaaatggg attgatggtt tattcactaa cgataattac
    11401 ggtaatcttg ctcctgcagt tgcagaggat tgggaagtct ctaaggatgg tttgacctac
    11461 acttataaga ttcgtaaagg ggttaaatgg tttacctctg atggagaaga atatgcagag
    11521 gtgacggcta aagatttcgt gaacggttta aaacacgcag cagataaaaa atcagaagct
    11581 atgtatttag ctgaaaattc ggttaaaggc ttggcagatt atctatcagg aacttcaaca
    11641 gatttttcaa cagttggtgt caaggcggtt gatgattata cgttacaata cactttgaac
    11701 cagcctgaac cgttctggaa ctctaagttg acctattcta ttttctggcc tctgaatgaa
    11761 gaattcgaaa catcaaaagg aagcgatttt gctaaaccaa cagatccgac atccttgctt
    11821 tataatggtc cattcttgtt gaaagggttg actgcaaaat cttctgtaga gtttgtaaaa
    11881 aatgagcaat attgggataa agaaaatgtc cacctagata ctatcaatct agcttactat
    11941 gatggatcag atcaggagtc gctagagcgt aacttcacta gtggagctta tagttatgcc
    12001 cgtctttacc ctaccagctc caactattct aaggttgcag aagaatacaa ggacaatatc
    12061 tattacacac aatcaggctc tgggattgct ggtctgggtg tgaatattga tcgccaaagt
    12121 tacaactata cttctaaaac tacagattca gagaaagtag ctactaagaa ggcattgctt
    12181 aacaaagatt tccgtcaagc cttgaatttt gctcttgatc gctcagctta ctcagctcaa
    12241 atcaatggta aagatggagc agctttagca gttcgtaatt tatttgtaaa accagacttt
    12301 gtttcagctg gtgagaagac ctttggtgat ttagtcgctg ctcaacttcc tgcttatggt
    12361 gatgagtgga aaggtgtgaa tttagctgat gggcaggatg gtttattcaa tgttgacaag
    12421 gccaaggcag agtttgcgaa agctaagaaa gctttagaag cagacggcgt tcagtttcct
    12481 attcatctgg acgttccagt agaccaagca tcaaaaaact acatatctcg tattcagtcc
    12541 tttaaacaat ctgtagaaac agttcttggt gttgaaaatg tcgttgttga tattcaacaa
    12601 atgacaagtg atgaattcct taatattact tactatgctg ccaatgcttc atctgaggat
    12661 tgggatgtat caggaggagt ttcatggggt ccagactatc aagacccatc tacttacctg
    12721 gatattttaa aaacaactag cagtgaaact acaaaaacat atttaggatt tgataatcca
    12781 aatagccctt cagtagttca agttggtttg aaagaatacg ataaattagt tgatgaagct
    12841 gccagagaga caagcgactt gaatgtccgt tatgaaaaat atgcagcggc tcaagcatgg
    12901 ttgacagata gttcactctt tattcctgct atggcttctt ctggtgcagc accagtgctt
    12961 tcacgaattg ttccatttac tggagcttct gcgcaaacag gctctaaggg gtcagatgtt
    13021 tacttcaaat atttgaaatt acaagataaa gtggtgacta aggaagagta tgaaaaagct
    13081 cgtgaaaaat ggttgaaaga aaaagctgaa tcaaatgaga aagctcaaaa agaattggca
    13141 agtcatgtga agtaaataat ttctaataga actttctctc catgaggaga aggttctttt
    13201 gggattttta aaggaaataa tatgaaaaaa tatattttta tgcgtgtttt gcggtcattg
    13261 gtttcgattt tcttagtaac gactttgacc tacacgatta tctatacctt ggttcctcga
    13321 aaattgattt tcaagcagga tcctaactat aataaaattg cgacaacggc tgataaacgt
    13381 gataactatg aaaatactgt gtttgagcgt atgggctaca ttgagtatta cgatactaaa
    13441 gagttgcaag aaaaggcaag tagcatggat tcttctgtaa cagtagaagc aaatgcgacc
    13501 aataaagcta tttatgaaaa gtacatcaat caattaggtc atggttggac tttgggagaa
    13561 tttactgaaa gtggtcaatt ctatgctact cgtgaaattc caatttttga acgtgttttt
    13621 cacttctatg ctaacttgat tgacattgac catacaaata aaatccaaga ccctgaaaat
    13681 ccagacttga aacgctacct tcgttttgaa aatgatccag ctatcggatg gtcattggtc
    13741 ggttcaggaa ctaaacataa atatctcttg tactttaaca gtcagttccc atttgttcat
    13801 caaaactttg tgaacttgaa tttaggtgac tcttacccaa cctatgctaa tacaccagtt
    13861 cttcaggtta ttactcaagg tcaaggacaa accaaaactg cccaagttca gttcccaaca
    13921 ggtaagaaaa cgtcttctgt aaatatttac tcaagaacct acaagtcacc tagtcaggct
    13981 gactctcgtg aagtagctag ctatgggaaa gatgatcctt atacagcgac tgaaagtaat
    14041 taccaatatc catctatgat tgtcagctct gctattactg gtttgattgg tttggttctt
    14101 gcctatgctc ttgccgtgcc acttggttca gccatggctc gtttcaagaa cacttggatt
    14161 gatagcctct caacagggac tttgaccttc ttgcttgctc ttccaacgat tgccttggtt
    14221 tacatcgttc gattgattgg atcatctatt gcccttccag attcattccc tatcttggga
    14281 gctggagatt ggcgttctta cgttttacca gcagtcatcc ttggtttgtt gggtgctcct
    14341 gttacagcca tttggattcg tcgttacatg attgacttgc aatctcaaga ctttgttcgt
    14401 ttcgctcgtg caaaaggttt gtctgaaaaa gaaatttcaa acaaacacat ctttaaaaat
    14461 gccatggttc cgctggtttc aggaattcct ggtgccatta ttggggttat cggtggtgca
    14521 acccttactg aaacagtctt cgccttccca ggtatgggta aaatgttgat tgactctgta
    14581 aaagcatcta ataactctat ggtcgttggt cttgtcttca tctttacatg tatttctatc
    14641 ttctcacgtc ttttgggaga tatttggatg actattattg acccacgtat taaattgact
    14701 gagaaaggag gcaaataatg tctacaatcg ataaagaaaa atttcagttt gtaaaacgtg
    14761 acgattttgc ctctgaaact attgatgcgc cagcatattc ttactggaaa tcagtgttta
    14821 aacaatttat gaagaaaaaa tcaactgtag tcatgttggg aatcttggta gccatcattt
    14881 tgataagttt catctaccca atgttttcta agtttgattt caatgatgtc agcaaggtaa
    14941 acgactttag tgttcgttat atcaagccaa atgcggagca ttggttcggt actgacagta
    15001 acggtaaatc gctctttgac ggtgtctggt tcggagctcg taactccatc ctcatttctg
    15061 tgattgcgac agtgattaac ttggttatcg gtgtttttgt cggtggtatt tggggtattt
    15121 caaaatcagt tgaccgtgtc atgatggaag tttacaacgt catctcaaac atcccacctc
    15181 ttttgattgt tattgtcttg acttactcaa tcggagctgg attctggaat ctgatttttg
    15241 ccatgagcgt aacaacatgg attggtattg ccttcatgat ccgtgtgcaa atcttgcgct
    15301 atcgtgactt ggaatacaac ttggcgtcac gtactttggg aacaccaacc ttgaagattg
    15361 ttgccaaaaa tatcatgcct caattggtat ctgttattgt gacaaccatg actcaaatgc
    15421 ttccaagctt tatctcatac gaagccttct tgtctttctt cggtcttgga ttaccgatta
    15481 cagtgccaag tttgggtcgt ttgatttcgg attattcaca aaacgtaaca accaatgctt
    15541 acttgttctg gattccattg acaacccttg tcttggtatc cttgtccctt ttcgtagttg
    15601 gtcaaaactt agcggatgct agtgatccac gtacacatag ataggagtag aaatgacaaa
    15661 agaaaaaaat gtaattttga ctgctcgcga tattgtcgtg gaatttgacg ttcgtgacaa
    15721 agtattgaca gccattcgcg gcgtttccct tgaactagtt gaaggagaag tattagcctt
    15781 ggtaggtgag tcaggatcag gtaaatctgt tttgacaaag accttcacag gtatgctcga
    15841 agaaaatggt cgtattgccc aaggtagtat tgactaccgt ggtcaggact tgacagcttt
    15901 atcttctcac aaggattggg aacaaattcg tggtgctaag attgcgacta tcttccagga
    15961 cccaatgact agtttggacc ccattaaaac aattggtagt cagattacag aagttattgt
    16021 aaaacaccaa ggaaaaacag ctaaagaagc gaaagaattg gccattgact acatgaataa
    16081 ggttggcatt ccagacgcag atagacgttt taatgaatac ccattccaat attctggagg
    16141 aatgcgtcaa cgtatcgtta ttgcgattgc ccttgcctgc cgacctgatg tcttgatctg
    16201 tgatgagcca acaactgcct tggatgtaac tattcaagct cagattattg atttgctaaa
    16261 atctttacaa aacgagtatc atttcacaac aatctttatt acccacgacc ttggtgtggt
    16321 ggcaagtatt gcggataagg tagcggttat gtatgcagga gaaatcgttg agtatggaac
    16381 ggttgaggaa gtcttctatg accctcgcca tccatataca tggagtctct tgtctagctt
    16441 gcctcagctt gctgatgata aaggggatct ttactcaatc ccaggaacac ctccgtcact
    16501 ttatactgac ctgaaagggg atgcttttgc cttgcgttct gactacgcaa tgcagattga
    16561 cttcgaacaa aaagctcctc aattctcagt atcagagaca cattgggcta aaacttggct
    16621 tcttcatgag gatgctccaa aagtagaaaa accagctgtg attgcaaatc tccatgataa
    16681 gatccgtgaa aaaatgggat ttgcccatct ggctgactag gaggaaggaa atgtctgaaa
    16741 aattagtaga aatcaaagat ttagaaattt ccttcggtga aggaagtaag aagtttgtcg
    16801 cggttaaaaa tgctaacttc tttatcaaca agggagaaac tttctcgctt gtaggtgagt
    16861 ccggtagtgg gaaaacaact attggtcgtg ctatcatcgg tctaaatgat acaagtaatg
    16921 gagatatcat ttttgatggt caaaagatta atggtaagaa atcgcgtgaa caagctgcgg
    16981 aattgattcg tcgaatccag atgattttcc aagaccctgc cgcaagtttg aatgaacgtg
    17041 cgactgttga ttatattatt tctgaaggtc tttacaatca ccgtttattt aaggatgaag
    17101 aagaacgtaa agagaaagtt caaaatatta tccgtgaagt aggtcttctt gctgagcact
    17161 tgactcgtta ccctcatgaa ttctcaggcg gtcaacgtca acgtatcggt attgcccgtg
    17221 ccttggtcat gcaaccagac tttgttattg cagatgagcc aatttcagcc ttggacgttt
    17281 ctgtacgtgc ccaagtcttg aacttgctca aaaaattcca aaaagagctc ggcctgacct
    17341 atctcttcat cgcccatgac ttgtcggttg ttcgctttat ttcagatcgt atcgcagtta
    17401 tttacaaggg tgttattgta gaggttgcag aaacagaaga attgtttaac aatccaattc
    17461 acccatatac tcaagccttg ctttcagcgg taccaatccc agatccaatc ttggaacgta
    17521 agaaggtctt gaaggtttac gacccaagtc aacacgacta tgagactgat aagccgtcta
    17581 tggtagaaat ccgtccaggt cactatgttt gggcgaacca aaccgaattg gcacgttatc
    17641 aaaaaggact aaactagtaa tggttttata atttccatgt caatttttat gggaattata
    17701 aaactttttt tgttggactg atttgaaaaa ctttgaaagc ttcctgaaag aattttcgaa
    17761 aaattttaaa aaattctgaa atattattga caggtgatga aaaaggtggt aggataggaa
    17821 gcatcaaaaa agaaagcaga aaaagaaatg aataagagac aagccgtaac tatgaatcaa
    17881 aacttttact ttagctactt tagatagtgt ttgtagacat gtcaccatgg atgcaaacag
    17941 ttcaagcaat ttgtttgtat ctatgtggct aagatttttg attgtggtcc atatagatga
    18001 atatctaaat ggactagcta agttgtaact tgttaggtta tttcaagtat ccgtttagat
    18061 agtatctagg cggttttttg ttttatcttt tctgagaagg agtttcatta tgaaagttgt
    18121 tcgaaaatca ctagccccct cttggtagtg gggctcctct tgacttctct gatcagtttg
    18181 catcagttga aggcagataa gaaaaagatg tctttcgtat cggtatttcg cagtttatta
    18241 cccaccagtc cttagacgct actagagaag gatttgtgga tgagctggcc aaacaaggct
    18301 atgttgaggg ggaaaatatt gagattgatt tgcaaaatgc acagggagaa caaagaaatc
    18361 taaaaacgat ttcccagcaa ctagcagaat ccagcgatgt cgttctagcc atcgcaaggc
    18421 cttctgctca gagcttggcc aatacgacgc aaacgacacc agttatcttt tcagctgtaa
    18481 cagacccagt cagtgccaag ttggttgagt caagagaaca tcctgggggc aatgtaactg
    18541 ggacaagcga tcagtcatca gatgccattt caacccaaat caatttgata aagaaagtgt
    18601 tgccaaaggc taaaacgatt ggaatcctct atactcagag cgagcccaat tcggttgttt
    18661 aaaaggatga agctaagcga cttctgaaag aaaaaggctt taccgttgtt gaaaaaacaa
    18721 tcttggacag taataacgtc aaggcggcag cagagagttt gatggcagag gtggatatgg
    18781 tttttgtccc aactgataat atcatttcgt caaccatgga aacagttaag caggtttcta
    18841 ttaaacacaa ggttccagta ttcggtggtt caacagaaat gattgctgtt ggtggcttgt
    18901 ataactacgg aaccaattat gaagaattgg gacggcagac agctcgcatg ctgattcgtg
    18961 ttttaaaagg tgagaaacca gaaaatatag cagttgagtt gcctgaaaaa ctggaattgc
    19021 ataccaatca agaaatggca gatgcattgg gaattgatat tagtaagtta gaaggcaagg
    19081 aataaggagg attaagatga attttgtgtt atctagttta tcagaaggtt tgttgtggtc
    19141 gattatggca attggggtct acttgacttt ccgtattttg aatattgcgg atatgactgc
    19201 tgagggagcc tttcctctgg gggcagcagt cgttgtatca cagatacagg caggggcaaa
    19261 tccttggctt gcgactttac ttgctttggt ggcaggtatg gtagcaggtc ttgtatcagg
    19321 aatgctccac accaatatga aaatcccagc tctcttgaca gggattgtga ccttgacagg
    19381 gctctattca atcaatatta aaattatggg cagtgtaccc aatctttcct tgggagattc
    19441 tgcaaccgtc tttaaacagt tggcgagctt gggtctgaca aatgaaggag ctgttttctc
    19501 acttagttta gtctgtctct tacttgtttg gtcttgaccc ttttgatgaa aacagagatt
    19561 ggtttggtct tgcgttcgac tggggacaat atcccgatga gtgaggccaa tggagtcaat
    19621 gtaaacacca tgaagattgt tggttacatg atttcaaacg gtttgattgc cctatgtggt
    19681 tccttgtttg cccaaaatga tggattttca gatgtgactt ctgggacagg aaccattgtt
    19741 gttggtttga gctcggaagt cttgatacac gacttgacca ttggaggcca cttattatcc
    19801 atcggaatcg gtgctattgt ttaccgtttg attattttaa atatctatga aattccaaat
    19861 ctagatcaaa acttggtacg tctctttaat gcaatcttgc tcgctttggt tttatttgca
    19921 ccagaattgc aaaagagatt aaagattcgt ggtctgaaat tgagaaatga ataggaggag
    19981 aaagttatgg caagtttgtt aacacttgaa aatattcaca agacatttga agcagggacg
    20041 gtcaatgaga accatgtcct caaaggatta gatttagagg ttgaagaggg agattttatc
    20101 tctgtgattg gtagaaatgg agcaggaaaa tccactttga tgaatatctt ggctggtaat
    20161 ctatcggtgg acgaagggga ccttctactg gcagggaaat ccattaaaca tctgagtgtc
    20221 agaaagagag ccaaggatat tgcccgtgtt tttcaagatc ctaagatggg aacagcttct
    20281 cgtttgacga ttgaggagaa tatggctatt gccttgagac gtgggcaaaa gagagggctt
    20341 ggttggggcg tgaaggagaa ggatagaatc cagttccaag aggccttgaa ggagttgaat
    20401 attggtcttg aaaaccgctt aaaagtagat actcaatatc tctcaggagg acaaagacag
    20461 gctttgaccc tagtcatggc agctctggtg aaactcaaac ttttgctttt ggatgaacat
    20521 actgcggcac ttgacccgaa aaccagtcaa atggtgatgg acttgacgca aaagattgtg
    20581 gagtaccatc agtggacgac tttgatgatt acccatgata tgaatcatgc gattgagtac
    20641 ggcaatcgtc tcattatgct ctatcaaggc aagatagtgg tggatgtcaa gggagaagag
    20701 aaaaagcatc tgacagttga agaccttatg catctcttcc agaaaaatag tggccaaagt
    20761 ctggtcagtg atgaattagt tttgggataa agaaaaaggc tgagatctgg tgtctcagtc
    20821 ttttatttct tgcgttttgc aaaatctaca aatctaaatt tttctaactt atggcgactt
    20881 tcggtaaact gaaattggcg cccgtcttgg agatagactt ttgatttaat cgaaacgaca
    20941 taagggtctt tgccaatgtc catgagaatc ttgtctcggt cgcttgagtg gtcaatggtg
    21001 atttccttct gagcataatc aataaggagt ttgaggccat tttctatata agaatagata
    21061 gactgctcag caatttggcg agtgagattt gggatgagtt ccatatccag atagtctgta
    21121 tccagaacgg ataccagatc atccaccaca cgctggcgga ccaccttcca aaccatccga
    21181 aactctggga aaccggttat cagtgaggat tttttatcaa taataatctt gtccagactg
    21241 accacgttgg ttttagagcg cagtccaagt tctttaacta gttcttggta gctggttagg
    21301 ttggatacag ggaaattgac ggtttcttct ttgacgactt gagaaccttg ccctcttatc
    21361 tttttgatca atccttcctc ttggaggaga gacagggcct ttcggacggt atcacgactc
    21421 acttgatatt gctccataag gtcgtgctcg ctaggaagga aatctccgac agcgtaagtc
    21481 tcgttttgaa tggtttcttg gatttgctta aataattgtt ggtatttctt cattttgcct
    21541 gcttaaatag gttttcagtc tagtttaatg aacacctgta tctattttac catacaagtg
    21601 ctagaaaatt tacaaaaaaa cacccttttt ataccaaaca agttgcagac aagtttggta
    21661 tcgtttacaa tatacttatc aaatcaaact tgctttgaca agtataagga gaatcaaatg
    21721 ggaaaatttg aacaagaagc caaagatctg cttcaggcaa ttggaggcaa agaaaatgtg
    21781 actgccgtaa ctcactgtgc gacacggatg cgctttgttt taggagatga taagaaggct
    21841 aatgttaaag ctatcgagtc aattccagct gttaaaggaa cttttacaaa tgcaggtcaa
    21901 tttcaggtaa tcattggaaa tgacgtgccc atcttttata atgattttac agccgtttca
    21961 ggtattgagg gtgtttccaa agaagcagcc aagtctgcag ctaagagtaa tcaaaacgtg
    22021 gtccaatgtg ttatgaccac tctggcggag atttttactc cgattattcc agccttgata
    22081 gtcggaggat tgatcctcgg tttccgtaat gtcttggaag gtgtccattg gtcgatgttg
    22141 gatggcaaga ccatcacaga atcctctcag ttttgggcag gggttaatca cttcctttgg
    22201 ttgcctggtg aagctatctt ccagttctta ccagtaggga ttacttggtc tgtttctcgt
    22261 aagatgggaa ccagccaaat tttgggaatt gttctcggaa tctgtttggt atcgcctcag
    22321 ttgctcaatg cctatgcggt tgcttcaacg ccagcagctg atatcgcggc aaactgggtt
    22381 tggaattttg gctattttac tgttaatcgt atcggttacc aagcccaagt tatcccagcc
    22441 ttgcttgcag gtttgagtct gtcttatctt gaaatcttct ggcgcaagca tatcccagaa
    22501 gtcatttcta tgatttttgt acctttcttg tcattgattc cagccttgat tttggctcat
    22561 actgttttgg gaccaatcgg ttggacaatt ggacaaggac tttcatcagt tgtcttggca
    22621 ggtttaactg gtccagttaa atggctcttc ggtgcaattt ttggtgccct ctacgctcca
    22681 tttgtcatca caggtctgca ccatatgacc aatgccattg atacacaatt gattgcggat
    22741 gctggtggca ctgccctatg gccaatgatt gctctttcta atattgctca aggctcagcc
    22801 gtgtttgcct attatttcat gcatcgccat gatgagcgtg aggctcaggt ttcacttcct
    22861 gcaaccattt cagcctatct cggtgttaca gaaccagctc tttttggggt taacgtaaaa
    22921 tatatttatc catttgttgc tgggatgact ggttcagccc ttgcaggcat gttatccgtt
    22981 acttttaatg taactgcggc ttctattggt atcggtggtt tgccaggtat tctctctatt
    23041 caacctcaat acatgctgcc atttgcagga actatgctag ttgcgattgt tgttccaatg
    23101 ctcttgactt tcttcttccg caaggctggt ctctttacaa aaacagaggg cgatacgaac
    23161 ttgcaggcag aattcgttgc tcaagaagaa gcagaatttg tgaaccatga accagtagaa
    23221 cttacttcgg tagaaattat cagcccacta actggccaag tgaaagaatt gagtcaagcg
    23281 acggatccta tttttgcatc aggtgtcatg gggcaaggtc tggtcattga accaagccaa
    23341 ggtgagttga cctctccagt taatgggaca gtgacggttc ttttccctac caagcatgcc
    23401 atcggcattg tctctgacga gggagttgaa ttgctcatcc acatcggtat ggatacagta
    23461 ggtcttgatg gcaaaggttt tgaaagtctt gtagtccaag gagatcacgt tacagttggt
    23521 cagcaactga ttcgttttga tatggatgtc attaaggctg caggtctggt gacagaaact
    23581 cctgttatca tcaccaacca agatgcttat acagcgacta ttcccggaac ttatccgaca
    23641 acgatccaag ctggagcatc tctcatggtc gctacacgaa tctaatactc ttcgaaaatc
    23701 tcttcaaacc acgtcagctc tatctgcaac ctcaaaatag tgttttgagc aacctgtggc
    23761 tagcttccta gtttgttctt tgattttcat tgagtataat tgaaatagca caaataggga
    23821 cggaagactg cttcctccct tttatcagaa aaggagaaac agatgacact tgataaagga
    23881 aaagtagtct atcaaatcta tccaaaatct tacaaagaca ccactgaaaa cggtattggg
    23941 gatttccgtg ggattatcga aaaaattccc tatttagcca aattaggtgt ggatatggtc
    24001 tggctcaatc cattctatcc aagccctcaa cgggataatg gttacgatat ttcagattat
    24061 atggcagtgg atcctctttt tggtgatatg gctgattttg aggaaatggt gtgtgtcggt
    24121 aaagagcaca agattgactt tatgctggac atggtactta atcattgttc gacagagcat
    24181 gaatggtttc agaaagccct agctggtgat aagtattatc aagacttttt cttcatccaa
    24241 gaccaaccaa cagattggca gtctaagttt ggtggttctg catgggcgcc tttcggggat
    24301 actgggaaat actatcttca cctatttgat gagacccagg ctgaccttaa ctggcgcaat
    24361 tccaatgtcc gtaaggagct tttcaaggtt gttaatttct ggcgcgacaa gggtgtcaaa
    24421 ggttttcgat ttgatgtgat caatttgatc ggtaaggacg aggtttcagt ggactgtcct
    24481 gaaaatgaag ggaagccagc ttatacagac aaacccatcg ttcataacta tttgcgtatg
    24541 atgaaccaag ccacttttgg atctgacgat agctttatga cagttgggga aatgtcttct
    24601 accaccatgg aaaactgtgt cctctattcg tcgcctgatc gtcaggaatt atccatgacc
    24661 tttaattttc atcacctcaa ggtggactat aaggatggac aaaagtggac cttggctccc
    24721 tttgattttg aagagttgaa gagtctttac catagttggg gtaaggaaat gagtgataaa
    24781 gacggttgga gtgccctctt ttggaacaat cacgatcaac cacgtgcctt gaaccgtttt
    24841 gtcgatattc aaaacttccg caaggaagga gccactatgc tagcagccag cattcacctg
    24901 tcacgtggga caccttatat ctacatgggt gaggaaatcg ggatgattga cccagactat
    24961 gattctatgg ctgattacgt ggatgtcgaa tcgcttaatg cctatcagat gctcctagaa
    25021 gaaggaaaga gtcagcaaga agccttccag attattcaag ctaagtcgcg tgataattca
    25081 cgcattccca tgcagtggga tgcttcggaa aatgcaggat tttcaacagg cactccttgg
    25141 ttgaaagctg gtaaatccta taaagacatc aatgtagaaa atgaaatcca gggtccgatt
    25201 tttaccttct atcaagactt gattcgactg cgtaaggaaa tgccaatcat ttcagaagga
    25261 agttacaaac cagcctttga agatagcaaa caagtctacg cttttgaacg ccagtttgag
    25321 gatcaaaagt tactagtgct caataacttt tatgctaaag aagtggaaat cgacttgcca
    25381 gcagtctacc aaaatggaca gattttgatt tcaaactatg aagatgcaga agtgtctgag
    25441 aaaattctac taaaacccta tcaaacactg gctatttatg taaattaaac ttagcaagga
    25501 gacctctttt tcagaaaagc agggttcttt ttgctgtcta ttccatcctt gaaagtgtag
    25561 aacaaactgg tcagggtggc tagtttgtgg tataatgaaa gggattcaaa aagaaacagg
    25621 agaaaaatga tggatttact tttagcaatt gtattgattg tgctagcttt tctaggagga
    25681 gctcttggag gaatgtactt ggttcgtaag caaatcgaaa aagaatttgc tgacaaccca
    25741 cgtttgaatg ctgaagcagt tcgtactctt ttgagtgcaa atggtcaaaa accaagcgaa
    25801 gctaaggtac aacaagttta ccaccaaatc atccgccaac aaaaggcagc ccttgctaac
    25861 aataaaaaga aaaaataaat aaggaaaagt ctgggatgaa agttccagac ttctcactat
    25921 gttggttatg gtttagggat gagacttttt ccttgcattc tattgatatt tgattatgat
    25981 taagagagag gtagttgaca ttctatcaag ctgtcggtca gttcgttcag tacaaggaat
    26041 cataatgaac gatcaatcag aaaaaagact agaaagaaga ctgtatggat aatcgaccaa
    26101 ttggtttttt ggattcgggt gtcgggggct tgaccgttgt gcgcgagctc atgcgccagc
    26161 ttccccatga agaaatcgtc tatattggag attcggcgcg ggcgccctat ggcccccgtc
    26221 ctgctgagca aattcgtgaa tatacttggc agctggtcaa ctttctcttg accaaggatg
    26281 tcaaaatgat tgtcattgct tgtaacactg cgactgcggt cgtctgggaa gaaatcaagg
    26341 ctcaactaga tattcctgtc ttgggtgtaa ttttgccagg agcttcggca gccatcaagt
    26401 ccagtcaagg tgggaaaatc ggagtgattg gaacgcccat gacggtacaa tcagacatat
    26461 accgtcagaa aatccatgat ctggatcccg acttacaggt ggagagcttg gcctgtccca
    26521 agtttgctcc cttggttgag tcaggtgccc tgtcaaccag tgttaccaag aaggtggtct
    26581 atgaaaccct gcgtcccttg gttggaaagg tggatagcct gattttgggc tgtactcatt
    26641 atccactcct tcgccctatt atccaaaatg tgatggggcc aaaggttcag ctcatcgata
    26701 gtggggcaga gtgcgtacgg gatatctcag tcttactcaa ttattttgaa atcaatcgtg
    26761 gtcgcgatgc tggaccactc catcaccgtt tttacacaac agccagtagc caaagttttg
    26821 cacaaattgg tgaagaatgg ctggaaaaag agattcatgt ggagcatgta gaattatgac
    26881 aaataaaatt tatgaatata aggatgacca ggactggtat gttgggtctt atagtatttt
    26941 tggtggcgtt aacagtttga gcgactataa gacagatttt cctctgtttg aattctccaa
    27001 aatatttgga gatgaagagt atggtttccc gctttcagtt actgttttac gctatggttc
    27061 tacctaccgt ttgttctcct ttgtggtaga catgcttaat caagaaatgg gacgaaactt
    27121 ggaagttatt caacgtcatg gggccctgct cttggttgaa aatgggcaac tcttgtatgt
    27181 agaattgcct aaagaagggg tcaatgttca tgatttcttt gagacaagca aggtcagaga
    27241 aaccttgttg attgcgactc gtaacgaagg taaaaccaag gaattccgag ctatctttga
    27301 taagttaggc tacgatgtgg aaaatcttaa tgactaccct gacctgcctg aagtagcaga
    27361 aacaggtatg acctttgaag aaaatgcccg ccttaaggca gaaaccattt ctcaattaac
    27421 gggcaagatg gttttggcag atgattctgg tctcaaagtc gatgtccttg gtggcttacc
    27481 aggcgtctgg tcagctcgtt tcgcaggtgt gggagcaact gaccgtgaaa ataatgccaa
    27541 actcttgcac gaattggcca tggtctttga actcaaggac cgctcggctc agttccacac
    27601 aaccctagtc gtagccagcc caaataagga aagtttagtt gttgaagcag actggtcagg
    27661 ttatattaac tttgaaccta agggtgaaaa tggctttggc tatgatcccc tcttccttgt
    27721 aggagagaca ggtgagtcat cagctgaatt aaccctggaa gaaaaaaata gtcaatctca
    27781 ccgtgcctta gccgttaaga aacttttgga ggtatttcca tcatggcaaa gcaaaccatc
    27841 attgtaatga gcgattccca tggcgatagc ttgattgtgg aagaagtccg tgatcgctat
    27901 gtgggcaaag tcgatgccgt ttttcataac ggcgattctg aactacgtcc ggattctcca
    27961 ctttgggagg gcatccgcgt tgttaaaggg aacatggact tctacgccgg ctacccagaa
    28021 cgtctggtga ctgagcttgg ttcgaccaag attatccaaa ctcatggtca cttgtttgac
    28081 atcaatttca actttcaaaa gttggactac tgggctcagg aggaagaggc cgctatctgc
    28141 ctctatggtc acttgcatgt gccaagtgct tggttggaag gcaagatcct ctttctaaat
    28201 ccaggttcta tcagtcaacc acgaggtacc atcagagaat gtctctatgc tcgtgtggag
    28261 attgatgata gttacttcaa agtggacttt ttgacacgag atcacgaggt gtatccaggt
    28321 ttgtccaagg agtttagccg atgattgcca aggagtttga gactttcttg ttggggcagg
    28381 aggaaacttt tttgacccct gctaaaaatc tagctgtgtt gattgatacc cacaatgcgg
    28441 atcatgcgac cctcttgctc agtcagatga cctatacccg tgttcccgtt gtgacagatg
    28501 aaaaacagtt tgttgggacg attggactca gagatattat ggcttatcag atggagcatg
    28561 acttgagcca agaaatcatg gcggatacgg atatcgttca tatgacaaaa acggacgtag
    28621 cggttgtttc gcctgatttc accattacgg aggtcttgca caagctagta gatgagtcct
    28681 tcttaccggt tgtggatgca gagggtattt tccaagggat tattacgcgc aagtccatcc
    28741 tcaaggccgt taatgccctc ttgcatgact ttagtaagga atatgagatt cgatgccaat
    28801 gagagacagg atttcagcct ttttagagga aaagcagggc ttgtctgtca attccaagca
    28861 gtcctataag tatgatttgg agcaattttt agacatggta ggtgagcgga tttctgagac
    28921 cagtctcaag atttaccaag cccagctagc caatctaaaa atcagcgccc agaagcgaaa
    28981 gatttcggcc tgtaaccaat ttctatactt tctctatcaa aaaggagagg tggacagctt
    29041 ttaccgcttg gaattagcca aacaagctga aaagaagacg gaaaagccag agattctata
    29101 cctagactct ttttggcagg aaagcgacca tccagagggc cgcttgctag cgctcttaat
    29161 cctagaaatg gggcttttgc ccagtgagat tttagccatc aaggttgcgg acatcaatct
    29221 ggattttcag gtgttgcgaa tcagcaaggc ttcccaacag aggattgtca ctattcccac
    29281 ggccttgctt tcagaattgg aacccttgat ggggcagacc tatctttttg aaagaggaga
    29341 gaaaccctat tctcgtcagt gggcctttcg tcagttagaa tcttttgtca aggagaaagg
    29401 ttttccatcc ttatcagctc aagtcttacg tgaacagttt attctaagac aaatagaaaa
    29461 caaggtcgat ttgtacgaaa ttgcaaaaaa attaggatta aaaacagtcc tgaccttaga
    29521 aaaatataga taatggatat taaattaaaa gattttgaag gacccctgga cttgctcttg
    29581 catctggttt ctaagtacca gatggatatc tacgatgtgc ccattacgga agtcatcgaa
    29641 cagtatctag cctatgtctc aaccctgcag gccatgcgtc tggaagtgac gggtgagtac
    29701 atggtcatgg ctagtcagct catgctgatt aagagtcgta aactccttcc gaaggtagca
    29761 gaagtgacag acttggggga tgacctggag caggacctcc tctctcaaat cgaagaatat
    29821 cgcaagttca agctcttggg tgagcacttg gaagccaagc accaagaacg ggcccagtat
    29881 tattccaaag cgccgacaga gttgatttac gaagatgcgg agcttgtgca tgacaagacg
    29941 accattgacc tctttttggc tttttcaaat atcctagcca agaaaaaaga ggagtttgca
    30001 caaaatcata cgacgatctt gcgggatgag tataagattg aggacatgat gattatcgtg
    30061 aaagagtcct tgattggacg agatcaattg cgcttgcagg atttgttcaa ggaagcccag
    30121 aatgtccaag aggtcatcac cctctttttg gcaaccctag agttaatcaa aacccaggag
    30181 ctgatcctcg tgcaagagga gagttttgga gatatctatc tcatggaaaa gaaggaagaa
    30241 agtcaagtgc ctcaaagcta gacttgatag agaggaaaga tgagtacttt agcaaaaata
    30301 gaagcgctct tgtttgtagc gggtgaagat gggattcggg tccgccagtt agctgaactc
    30361 ctctctctgc caccgacagg catccagcaa agtttaggaa aattagccca gaagtatgaa
    30421 aaggacccag attccagttt ggctttgatt gagacaagtg gtgcttatag attggtgacc
    30481 aagcctcaat ttgcagagat tttgaaggaa tactctaagg cgcctatcaa ccagagcttg
    30541 tctcgggctg cccttgagac cttgtccatt attgcctaca aacagccgat tacgcggata
    30601 gaaattgatg ccatccgtgg ggttaactcg agtggagcct tggcaaagtt gcaggctttt
    30661 gacctgataa aggaagacgg gaaaaaggaa gtattggggc gccccaacct ctatgtgact
    30721 acggattatt tcctagatta catggggata aaccatttag aagaattacc agtgattgat
    30781 gagcttgaga ttcaagccca agaaagccaa ttatttggtg aaaggataga agaagatgag
    30841 aatcaataag tatattgccc acgcaggtgt ggccagtagg agaaaagcag aagagctgat
    30901 taagcaaggc ttggtgacgg ttaacggcca agtggtgcgt gaactagcaa ccactatcaa
    30961 gtcaggcgac aaggtcgaag ttgaaggtca acctatctac aacgaagaaa aggtctacta
    31021 tctgcttaac aaaccacgcg gtgtgatttc cagtgtgaca gatgataagg gtcgcaagac
    31081 ggttgtcgac ctcttgccca atgtcaaaga acgtatttac cctgtgggtc gtttggactg
    31141 ggatacatca ggtgtcttga ttttgaccaa tgatggggac tttacagacg agatgattca
    31201 ccctcgtaat gagattgaca aggtttatgt cgcgcgtgtt aaaggtgtgg ccaataagga
    31261 caatctccgc cccttgaccc gtggtcttga gattgatggt aagaaaacca agccagctgt
    31321 ttatgaaatt ctcaaagtgg acccagtcaa aaatcgctct gtggtgcagt tgaccatcca
    31381 tgaagggcgt aaccatcagg ttaaaaagat gtttgaagct gttggtctcc aagtagataa
    31441 gttgtctcgg actcgtttcg gacacctaga cttgacagga ctccgtccag gagaatcccg
    31501 tcgtcttaat aaaaaagaaa tcagccaact acacaccatg gctgtaacta agaaataatg
    31561 aaacgaattt taatagcgcc tgtgcgcttt taccaacgtt ttatctcacc agtctttcca
    31621 ccctcttgtc gctttgagct gacttgttcc aactacatga ttcaggctat tgaaaaacat
    31681 ggttttaagg gggtattgat gggcttggct cggattttac gttgtcatcc ctggtcgaaa
    31741 acaggtaagg accccgttcc agaccacttt tcccttaaac gaaatcaaga aggggaatga
    31801 ggtggggtaa atagatttca aaatgataaa aacgcatcct atcaggtttg agtgaacttg
    31861 ataggatgcg ttttagaatg tcaaaatttt atactcttcg aaaatccctt caaaccgcgt
    31921 cagctttcat ctgcaacctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
    31981 tgctctttga ttttcattga gtattaaatt gagtttgaag tggcttattt caaagctttt
    32041 tgtatgtctt caatcatgag ttttgttgat tcaagtccgc ctccgcttag ataccagagg
    32101 tctggtgtta gttggataat cttaccattt ttagcagcag gtgtttcagc gataagggca
    32161 ttttctagga caccgtcgtt gctagagttg tccccaccga tggcaagggt acggttgatg
    32221 acaaagagga tgtcagggtt gatttctttg acactttcaa agctgacttc ttgtccgtgg
    32281 cgtgagtctt caaatgttgt atcagttggt ttgaatttca aggtttggta caagaaagag
    32341 aaacgagatt tggcaccaaa ggctgccatt tttccttcat taaggaggat cgcaagggcc
    32401 tttttgtcag agctttcatt tttagtagcg actttttgga tgctcttgtc tagcttggcc
    32461 aattcttcct tggctttctg tgtaccagtt tcgccgaagg cacttgctaa ggattcgata
    32521 ttagccttgg tagaagtcca gtagtcgtcc ttgcttgctt ggaagagaac ggttggggcg
    32581 atttctttga atttgtctac gaatttttgt gtacgtggtg aagcgataat caaatcaggc
    32641 tcaagggcgg cgatagcttc taaatcaggt tctttcatag aaccaacatt tttgacagtt
    32701 cccactaggt cttttagata agtcggaaca gtttttgtag gcattccgac gatatttttt
    32761 tcaaatccta aagcgcgaat agtatccgca gcgccgaggt caaaggtcac aatcttttca
    32821 ggaactttgg aaagtttgac cttgtccagt gaacttttaa tggttacctc tgttggagca
    32881 gagctactgg tctctgtctg actagtgctt gagtttgtac tacatgcacc aagtaggagc
    32941 aagaagctgg ccactagggc agtgaaataa agtttaaggg atgttttcat aatttctcct
    33001 ttttaaaatg tgataacgat ttagggagtc tcttaatctt attgactaag agactgaagg
    33061 ttctctaact tgagctttta tgttactagc tatagataca gatctttttg tcattgatat
    33121 cagctagcgt gatgggaatc tcataaagtt gactgagcag gtcagcctgc atgatttgat
    33181 cggttcttcc cttgctaaag acctggccgt ccttgaaggc gacaatttca tctgcatact
    33241 gactggccat gttgatatcg tggaggacga tgataatggt cttgccgagt tcctccacca
    33301 gtcgtcgaag aatctgcatc atgctgacgc tttgcttgat atcgagattg ttgagtggtt
    33361 cgtccagcaa gataaagtcc gtatcctggg ccagtaccat agcgataaag acgcgctgga
    33421 gttgcccccc tgacaggcta ttgatgtagc ggtcttttaa gttggtcagt tccaaatagt
    33481 tcagagtttc tcggattttt tcccagtctt ctgatctaag tcgacctcgg ctgtagggaa
    33541 aacgtccaaa actgaccagt tcttcaacag tcaatttggc ttggtaattg attttctgtt
    33601 ttaggatggt tagttcttgg gccagttctt gcgaattcca gctctcgatt tcacgtcctt
    33661 tgatactgag aactccctga tctttcttgg ttagtctgct catgatggag aggagagtcg
    33721 attttccagc accatttgga ccaataaagg ctgtcagttt ttgaggactg acttcaagcg
    33781 aaatgccttg caaaatatcc tgtttttgaa tggatttgtc aatgttttcc agtttcactg
    33841 acgagccctc ctatatagta agataaagaa taagaagcca cccacactct caatgatcat
    33901 actgatacga atttccagtg caaagactcg ttcaatcaag gcttgcccca aggttaagct
    33961 aataaatcca accagaatgg ccactataaa gagtaacttg tgctgatagt ctttgacaat
    34021 caggtaggtg aggttggcca gcataaagcc gaagaaggcc ataggtccta ccaaggcagt
    34081 ggccgttgag gtcaaaagca cgattcccca gaggagctct ttctgttctt tttcaacatc
    34141 gagtcccaat atctgagccg tttctctttg caggtgcaag acatctagaa cgactgcttt
    34201 tcgaaagaaa aagattgtca aagcgaggat gatcagagaa ccgatggcta ggatggaagt
    34261 gttaagatgt tgaaaggagg caaaaagact attttgcagt ttatcgtatt cgtttggatc
    34321 cattaggact tgaaggaagg tgctgatatt tcgaaagaga cttctgagcg ctagacagat
    34381 cagcaggatg aagaccaggt cttgcttcat cagtgtcttc aagtaacctt gtaaggcgag
    34441 aaagaagagg gactggacaa gaagtaagac taggaattct aagatagggg atttgccaag
    34501 ttgaagaaac ttgctttcaa aaaccagtag tagggtttgt agtaggacgt agaaggattc
    34561 aattcccaaa atactaggcg tcaggaagcg attttccgtc agggtttgaa aactaatggt
    34621 cgaaatccca gtcgcgatgg ctaccaagag ataaacgatg atcttttggg aacgcaactt
    34681 ccaagcaaag gctgacaagt gagtgatggg ccaaaagtag agaagacaag ctccgatggc
    34741 aagaataatg agaatccaga agagcttggt atgtttgctt ttagtctgca tcttttcgtc
    34801 cccctctcca gagaagtagg ataaagacga gactaccgat gattcctagc aagagactga
    34861 cagacaactc atagggccta atcagaactc gggataggat atcgcaagcc agaactagat
    34921 tggcaccaac cagtgcgacc atgagtttgg tttgacttag attatctcca tagcgcttgc
    34981 gaacaagatt gggaacgata actccgagaa atggtaagcc acccacggta atcatggtga
    35041 cgcttgtcgt tagcgccacc agaaagaggg ccagtttttc aagtagggag taggaaatcc
    35101 ccaaactctc gctggtttct ttccctagat tcatgatggt gaaggtttgg gataatttcc
    35161 aaacggttat caggatgatg aggcctaaga agagccactc atactgatgg gtctgaatca
    35221 tggagaagga gccctgggtc caggcagtca tactctgaac cagattgaaa cgataggcga
    35281 taacttctgt gactgagccg ataatcccgc tatagatgat cccaatcaga ggcaacatcc
    35341 acctttcctt tacagtaaaa atggtcataa aggctaggaa gaagagggtg aataggatgg
    35401 atgaaacaaa agcgaagagc atcttttggg tcagactagc cgatggaaag acaaaaaggc
    35461 tcagcaccat tcccagtttg gcggcttcag tcgttccaac tgtactcggt gcagcaaact
    35521 gattttgggt aatagtctgc atgagaaggc ctgccatact catactagag gcagtcagga
    35581 gaatactgat agttcttggg agacgggact cttgaaagag gagccaggtc tgctggtcga
    35641 aatcaaatag ctttccccat gaaaaatcac tggtcccaat gctaatagag agaaagacta
    35701 ggagtagaag taagccaatt aaataatgag aaagtttcat accccgtcct ttcatgtaga
    35761 tttggtatcg aaagatacct gcggatataa atgtaacatt atttttctaa tctgtcaata
    35821 aaatttctga caatttaata aatacaacaa ggagagagca acaagacttt ctcctttgtt
    35881 atcctattct aaaatgtttt taccttaatc tgataaaata atatcttcga gggagtagct
    35941 agccgtccaa tcaagatatt gtttagcttt tgaagcatct gctaggacac tggctgggtc
    36001 actagcacgt cgagcaacaa tctcgtgtgg gattttttaa tttagtaatt cttcagcagt
    36061 tttaaagatt tctttgatag tatagccttt tttagttcct aagttaaaga tttgagaaga
    36121 actgtcttct tgaaataggt agttcattcc tttaacatga gcctatgcaa ggtccaagac
    36181 ataaatgtaa tctcgaatac atgaaccgtc acgtgtatcg tagtcatctc caaatatttt
    36241 taagctatca ttttgtccca atgcggtctt gttgatattt ggaatgatgt gagttggatt
    36301 tttcacacgc agaccgtttg aagcatccat ttcagcccca gcaacattaa agtaacggaa
    36361 aataacatat ttccagtcgt agcgattggc catccagtaa atcattcgtt cgcccatcag
    36421 ttttgtctct gcataagggt tgacagggtc gagcagggta tcttcagtca ccggcttgtc
    36481 aatacagtta tttccataga gagaagcagt cgaagagaac atgatttttt gaatgccaac
    36541 ttcagataag actttgagaa cttggttcat accagcaacg ttggcagtga agtatttact
    36601 tggattttca atactttcgc tcacaacaat ttcacctgca caatggagga cagcatcaat
    36661 ctgattttct tctaagtaag cctttaaggc gctagcatca taaacatcca gttctttaaa
    36721 gctagcacga ctatccacag ccgctcgatt tcctgtggag agattatcga ggacatgcac
    36781 cttatagcca gcatttaaaa gagctttaac ggtatgggag ccaatgtagc tagtcccacc
    36841 tgtaacaaga attgttttgg tcatgatttt ttccttttct agttttgtac tatagtaaat
    36901 tgaaataaga tatgaacaac tctattagga aagtcaaatt aatttctaga aatgtttcag
    36961 cagctacagc gtactattcc aaactcaacc aactatatat ttaaagaaag aagaaaggga
    37021 agtcaactat tttctgtaaa tttcatgaaa aatccaaaca actttgatgg tgtattgttt
    37081 ttttgtttgg aaataaatat tcgagttaat tttggaatgt ttactagatt ttgcactaca
    37141 tgcaagagaa tactatcctt ttctaagata gagcaggact aagatactaa cttagaaatc
    37201 caccttccca ctatccttct cctataaaaa gtggtaaaat ggatgaaaga aagaaggaac
    37261 tgaaatgaca acattatttt caaaaattaa agaagtaaca gaacttgctg cagtctcagg
    37321 tcatgaagcg cctgtccgtg cttatcttcg tgaaaagttg acaccgcatg tggatgaagt
    37381 ggtgacagat ggcttgggtg gtatttttgg tatcaaacat tcagaagctg tggatgcacc
    37441 gcgcgtcttg gtcgcttctc atatggacga agttggtttt atggtcagcg aaatcaagcc
    37501 agatggtacc ttccgtgtcg tagaaatcgg tggctggaac cccatggtgg ttagcagcca
    37561 acgtttcaaa ctcttgactc gtgatggtca tgaaattcct gtgatttcag gttctgttcc
    37621 tccgcatttg actcgtggaa aggggggacc aaccatgcca gccattgccg atatcgtttt
    37681 tgatggtggt tttgcggaca aggctgaggc agaaagtttt ggcatccgtc ctggtgatac
    37741 catcgtacca gatagttctg caattttgac agccaatgaa aaaaatatca tctcaaaagc
    37801 ttgggataac cgctacggtg tcctcatggt aagcgatcta gctgaagctt tatcaggtca
    37861 aaaactcggc aatgaactct atctgggttc taacgtccaa gaagaagttg gtctgcgtgg
    37921 cgctcatacc tctacaacca agtttgaccc agaagtcttc ctcgcagttg attgctcacc
    37981 agcaggtgat gtctacggtg gtcaaggcaa gattggagat ggaaccttga ttcgtttcta
    38041 tgatccaggt cacttgcttc tcccagggat gaaggatttc cttttgacaa cggctgaaga
    38101 agctggtatc aagtaccaat actactgtgg taaaggcgga acagatgcag gtgcagctca
    38161 tctgaaaaat ggtggtgtcc catcaacaac tatcggtgtc tgcgctcgtt atatccattc
    38221 tcaccaaacc ctctatgcaa tggatgactt cctagaagcg caagctttct tacaagcctt
    38281 ggtgaagaaa ttggatcgtt caacggttga tttgattaaa cattattaaa ccataaggga
    38341 ggtggtaggg atggtagaac caaacctaga aagccttata aaagatcttt acaatcatgc
    38401 tcgacatgat ttgagtgaag atttagttgc tgctctccta gagactacta aaaaactgcc
    38461 tactacaaat gagcaattgc aggcagttcg tctctcaagc ctggtcaatc gtgaattgct
    38521 cctaaatccc aaacatccag cacctgagtt gctcaacttg gctcgctttg tcaaaagaga
    38581 agaagccaag tacagaggaa ctgcgacttc tgcgcttatg tatgaggaac tttttaaaat
    38641 gctttgattc cattgtggat caaagcattt tttataagac ctatctcgtg agcgttcaaa
    38701 tagtagtaac aaggaatcat gagaagtcag ccgagaccat agtagtgaag aaacttctgt
    38761 aatggaagtg gagtgaagtg aggaatcatc aaacggtagg ggataggctg tttgaagggt
    38821 gctacgaaaa agatagggta aggtgaaatt atagcggaat gaaataagat atgaacaaat
    38881 tgattaggga aatcaaatga atttatagaa atcttttagc agtcatggcg tcctattcta
    38941 gtttcaaaat gctataaata cgaacaaatt gattgggaaa ttcaaatcgc tttctatcaa
    39001 tgttttagaa gatacagtgc cctagtccaa cttcaatata ctataaaaga acagatggaa
    39061 gcaattatga tttacttcta tctttaaaat aaatgatctg ataggaaaaa tgtgtcccct
    39121 cataaaaaat aggatagtac ggatggaagg cttttgtaga ctggaggacg aaggaattta
    39181 gaaactctcg ttaaaagtta gttgagccgg agtatggtgg tgtgaataat tatcaaaaat
    39241 tatttccaaa tcgaaatagt tgacagttag aacgatgagt gttacaatta ttttattcat
    39301 ttcgatatcg aaataaattg gaggtgttat gaaagatagt catttgctag cccatcatat
    39361 tcgtttgttg aatgggcgga tttttcaaaa gttactgagt caagatcctg aggctcttta
    39421 taggggtgaa cagggcaaga ttttagcggt tttatggaat agtgaaactg gctgcgcaac
    39481 tgcgacagat atcgcgcttg cgactgggct tgcgaataat acgctgacga ctatgataaa
    39541 aaagctagag gaacaaaagc ttgtaattgt tagtccgtgt ggaaaagaca agcgtaagaa
    39601 gtatttagtt ttaacggagt taggcaagtt ccagaaagaa gtggggcatc gtgtcagtca
    39661 gaaattggat actatctttt acaaaggatt ttcagaggaa gaaattcacc aatttgaagg
    39721 ttttcaagaa agaattttgg caaatctgaa agagaaggaa aatgaggttt agaatatgtc
    39781 acaacaagtg aaaaatgctc acaacctata catccatgcc attcaagacg gacgagttgc
    39841 tgaggctcaa gcccagtctg taggggatac ctatatccaa cattcgacag gtgttcctga
    39901 cgggaaagaa ggatttgcag ccttctttgc agatttcttt gagcgtcatc cagagcgtca
    39961 gattaagatt gtccgcacca ttgaggatgg caatctggtc tttgttcatg ttcatcaata
    40021 tctgaatggt ggagaagctc aatgggtgac gacggatact ttccgtgcgg atgagaatgg
    40081 tcgtattgtt gagcattggg atgtcattga ctactatcga acacctgaaa atgaccaact
    40141 agatcaaatt tttggagatt ttgaaatcaa ggatttggac aagaaagcag aaaataaaaa
    40201 gctggttcgc cgtttcttga cagaaacttt ccaaaatggg gagctagagc agtggagtga
    40261 ttatgtggca gacgatttga ttcagcataa tcatgagatt ggacaaggaa gtgctgctta
    40321 taaaaactat gtggctgaat atagtgtcac ttttgacttt gttttccaac tcttaggaca
    40381 aggaaactat gtggttagct atggtcagac tcagattgat ggcgttgctt atgcccagta
    40441 cgatatcttc cgtttagaga acgggaaaat tgtggagcat tgggataata aggaagtcat
    40501 gcctaaggta gaagacttga ctaatcgagg gaagttttaa attgaggaca aagaatgatt
    40561 gaatacaaaa atgtagcact gcgctacaca gaaaaggatg tcttgagaga tgtcaactta
    40621 cagattgagg atggggaatt tatggtttta gtagggcctt ctgggtcagg taagacgacc
    40681 atgctcaaga tgattaaccg tcttttggaa ccaactgatg gaaatattta tatggatggg
    40741 aagcgcatca aagactatga tgagcgtgaa cttcgtcttt ctactggtta tgttttacag
    40801 gctattgctc tttttccaaa tctaacagtt gcggaaaata ttgctctcat tcctgaaatg
    40861 aaggggtgga gcaaggaaga aattacgaag aaaacagaag agcttttggc taaggttggt
    40921 ttaccagtag ccgagtatgg gcatcgctta cctagtgaat tatctggtgg agaacagcaa
    40981 cgggtcggta ttgtccgagc tatgattggt cagcccaaga ttttcctcat ggatgaaccc
    41041 ttttcggcct tggatgctat ttcgagaaaa cagttgcagg ttctgacaaa agaattgcat
    41101 aaagagtttg ggatgacaac gatttttgta acccatgata cggatgaagc cttgaagttg
    41161 gcggaccgta ttgctgtctt gcaggatgga gaaattcgcc aggtagcgaa tcccgagaca
    41221 attttaaaag tgcctgcaac agactttgta gcagacttgt ttggaggtag tgttcatgac
    41281 taatttaatt gcaacttttc aggatcgttt tagtgattgg ttgacagctc tatctcaaca
    41341 tttgcagttg tcacttttga ccttgttact agctattttg cttgcgattc ccttggctgt
    41401 ttttcttcgc tatcatgaga agctggccga ctgggtcttg cagattgcag gtattttcca
    41461 gaccatcccg tctctggcct tgttggggct ctttatccct ttgatgggaa ttgggacctt
    41521 gccggctttg acagctctag tgatttatgc gatttttccg attttacaaa atactattac
    41581 tgggctgaag ggaattgatc cgagtcttca agaggctggg attgcctttg ggatgaccag
    41641 atgggagcgt ctcaagaagt ttgaaattcc acttgccatg cctgttatca tgtctggaat
    41701 tcggacggca gcggtcttga ttatcggtac ggctaccttg gcgaccttga ttggggcagg
    41761 gggactgggt tcctttatcc tgttgggaat tgaccgtaat aatgccagtt tgattttgat
    41821 tggggccctt tcttctgcag tgctagctat tgcctttaac ttcctactaa aagtaatgga
    41881 aaaagcgaaa ttgcggacga ttttctctgg ttttgccttg gtggctttat tactgggtct
    41941 gtcttatagt ccagctcttt tggctcaaaa agagaaggaa aacttgatta ttgctgggaa
    42001 aataggccca gaaccagaaa ttttggctaa tatgtataag ttgctgattg aagaaaatac
    42061 cagcatgact gcgactgtta aaccgaattt tgggacgaca agtttccttt atgaagctct
    42121 gaaaaaaggt gatattgaca tttatcctga atttaccggt acggtgactg aaagtttgct
    42181 tcaaccatca cccaaggtga gtcatgagcc agagcaggtt tatcaagtgg cgcgtgatgg
    42241 cattgccaag caggatcatc tagcctatct caaacctatg tcttatcaaa atacctatgc
    42301 tgtagctgtt ccgaaaaaga ttgctcaaga atatggcttg aagaccattt cggatttgaa
    42361 aaaagtggaa gggcagctga aggcaggctt tacgcttgag tttaacgacc gtgaagatgg
    42421 aaataagggc ttgcaatcaa tgtatggtct caatctcaat gtggcgacta tgcagccagc
    42481 tcttcgttat caggcaattc agtcagggga tattcaaatc acggatgcct attcgactga
    42541 tgcggaattg gagcgttatg atttacaggt cttggaagat gacaagcaac tcttcccacc
    42601 ttatcaaggg gctccactca tgaaagaagc tcttctcaag aaacacccag agttggaaag
    42661 agttcttaat acattggctg gtaagattac agaaagccag atgagccagc tcaactacca
    42721 agtcggtgtt gaaggcaagt cagcaaagca agtagccaag gagtttctcc aagaacaagg
    42781 tttgttgaag aaatgatggg acagagactt taaacatact gaactcaact tccttaaacg
    42841 actagataaa agaatgctca gacaatttcg tctgggcttt tttgatgtga gaatgactga
    42901 atgtcactta aaatggaaag aaagagaaaa ttttcatgat tttctaaaat tttactgtaa
    42961 agacagttga ctattcataa aataggtgta taatattttg tgaactactt aacaaataag
    43021 aggttttagc tcatgtcgac taaggttaga aatcttgtgt ataggcagtt cattatctaa
    43081 tagagcgttg cgtccgaaag tctatccaga cacggctctt taaaaacaaa aggagaaatg
    43141 atgcatactt atttgcaaaa gaaaattgaa aatatcaaaa caaccctagg tgaaatgtca
    43201 ggtggttacc gtcgtatggt tgcggctatg gctgatttag gattttcagg aactatgaag
    43261 gctatctggg atgacctctt tgcccatcgt agttttgccc agtggattta tttgctggtt
    43321 ttaggaagtt ttcctctctg gctggagttg gtttacgaac atcgtattgt tgactggatt
    43381 gggatgattt gtagcttgac agggattatc tgtgtaatct ttgtatcgga aggtcgagca
    43441 agtaattatc tttttggctt gattaactct gttatttacc ttattttggc cctacagaaa
    43501 ggcttttatg gtgaggtgct gacgacactt tacttcacag tcatgcagcc aattggactt
    43561 ctagtttgga tttatcaggc acagtttaag aaggaaaagc aggagtttgt cgcgcgtaaa
    43621 ctggacggca agggctggac aaagtatctt tccattagtg tgctttggtg gttggccttt
    43681 ggcttcattt atcagtctat tggtgccaat cgtccctatc gtgattcaat cacagatgca
    43741 accaatgggg tagggcaaat cctcatgaca gctgtttacc gtgaacagtg gatattctgg
    43801 gcggctacca atgttttttc aatctatctc tggtggggag aaagcctgca aattcaaggg
    43861 aaatatctaa tttatctcat taacagtcta gttggttggt atcaatggag caaggcagct
    43921 aagcagaata ctgatttact taactaggaa aagatgtttg aaagtgctgt tttgagattt
    43981 cgattaaaac agatatagtt gataatcaag gatttatagt atgaaaaaga ggatcggcgg
    44041 gtcctctttt gttgttgaaa agataaaaaa ctcagtaacc tagaaataag acaactgagc
    44101 tttactctat attcaatttt taggaatgag aaggtctaga taaaattgga caacttcctg
    44161 gcctgtgaaa tcttgacctt ttttgagcca ccaggtcaat gtctcgataa agttggacac
    44221 gaccaagtgt tggaggtaag aagtaggcat attagggtgg gcttctttta aattatcagc
    44281 tagcacggaa tagacatggt gttctagctc tttatggagt tgacggagga agtagtcatt
    44341 tttggaaaat agcagactgg tgatatggtc ttggttttta tgaaaatgga gaaagaggtg
    44401 ggcgaggtag tcctcggttg aaatggcttg ctctctttca aaaagatgat ggaagaggta
    44461 gcggcagagc tggtccagaa gaagctcctt actttcatag tgacagtaaa aggtggatct
    44521 tcccacatct gcgagatcaa tgatatcctg aacagtagtg gcctcgtagc ccttagcatt
    44581 caaaagttgt ataaaagctt gatagatggc ttttttggtt ttgctgatac ggcggtcaat
    44641 gttagtcata tggacactta aggcaaattg ttcagaactg aataaagctg acgttttgct
    44701 tctatccttt ctttgagttt tagtggataa tgataatgaa caaggtgttc ataaatctat
    44761 tataacaaag gaatgagaaa tatgaaggca aaatatgctg tttgggtggc ttttttctta
    44821 aatttgactt atgccattgt tgagtttatt gcaggtggag tatttggttc tagcgctgtt
    44881 cttgctgact ctgtgcatga cttgggagat gcgattgcaa ttggaatatc agcttttcta
    44941 gaaacaatct ccaatcgtga agaagacaat cagtacacct tgggctataa gcggtttagc
    45001 ctgctaggag ccttggtaac agctgtgatt ctcgtaacgg gctctgttct agtcattttg
    45061 gaaaatgtca cgaagatttt gcatccgcaa ccagtcaatg atgaggggat tctctggtta
    45121 ggaattattg cgattactat caatctgtta gcgagtctgg tggttggtaa gggaaagaca
    45181 aagaatgagt ctattctgag tttgcatttt ctggaagata cgctagggtg ggtagctgtt
    45241 atcctgatgg cgattgttct tcgatttacg gactggtata tcctagatcc tcttttgtcc
    45301 cttgtcattt ctttctttat tctttcaaaa gcccttccac gtttttggtc tacactcaag
    45361 attttcttgg atgctgtgcc agaaggtctt gatatcaagc aagtaaagag tggcctggag
    45421 cgattggaca atgtggccag ccttaatcag cttaatctct ggactatgga tgctttggaa
    45481 aaaaatgcca ttgtacatgt ttgtctaaaa gaaatggaac atatggaaac ttgtaaagag
    45541 tctattcgaa ttttcctaaa agattgtggt tttcaaaata ttaccattga aattgatgct
    45601 gacctagaaa ctcaccaaac ccataagcga aaggtgtgtg acttggaacg gagttatgag
    45661 catcaacatt agaaaaaagt gaaaaatact tgggtactat cttatttgga atagagtaat
    45721 ttctttatta tttaaatatt tcaaaaattg gtaagagaag agcattgtat aaactccaga
    45781 tatatgattg ttaatgataa aaatttttcg attagataca aaatgcttga cttggagtca
    45841 actcaaagtt atataataag ataagtgagt tagaatagcg tgaattcagt gaatgaaatg
    45901 agaggaggtt agcgtgtgaa tattaaatct gccagtgatt tgttgggaat ttcagcggat
    45961 acgattcggt attatgaacg ggttggtctt gtgccaccga ttactcgtac tgctactggg
    46021 attcgtgatt ttcaagatca ggatatcgaa gcgctggaat ttattaagtg ttttcgttcg
    46081 gcgggtgtct ctgtagatag tttagttgac tatatgtcgc tctaccaaaa gggagatgaa
    46141 acgagagagg agaggcttgg tattttagaa gaggaaaagc aaaaattaga ggagcgcttg
    46201 tctcagctac agacagcttt aaatcgttta aatctcaaaa ttaaacttta taaggaagga
    46261 aaattttaaa tgaaatcagc agtatataca aaggcaggtc aggttggact tgctagcatt
    46321 gaacgtccgc aaataataga agcggatgat gtgattattc gtgtggttcg tgcgtgcgtt
    46381 tgtggttcag atttatggag gtaccgtaat ccagaaacga aagctggaca taaaaatagt
    46441 ggacacgaag cgattgggat tgttgaagaa gctggggaag ccattacgac ggtgaaacca
    46501 ggtgattttg tgattgtccc ttttacacat ggatgtggtg agtgtgatgc ctgtcttgct
    46561 ggatttgacg gttcttgcga caatcatatt ggtaataatt tggggggtga ttttcaggca
    46621 gaatatatcc gtttccacta tgccaactgg gcgctggtta aaattccagg acaaccttca
    46681 gattatagcg aaggtatgct aaagtccctc ttgactcttg ctgatgtgat gccaacaggc
    46741 tatcatgcgg cgcgcgttgc aaatgttcaa aaaggtgata aggttgtagt cattggcgat
    46801 ggggctgttg gtcaatgtgc tgttatcgcg gctaagatgc gtggtgcttc acaaattatt
    46861 cttatgagcc gtcatgaaga tcgtcaaaag atggctctgg agtcaggtgc gacagccgtt
    46921 gtagctgaac gtggtcaaga aggaattgcc aaggtgcgtg aaatcctagg tggaggagca
    46981 gacgcagcac ttgaatgtgt tggaacagaa gcagcgattg atcaagcact tggtgttctc
    47041 cataatggag ggcgcatggg ctttgttggt gtaccgcact atgaaaatcg agccattggc
    47101 tcaacctttg gtcaaaatac aataatcggc ggtggtccag cttctgtcac aacttatgac
    47161 aaacaagtat tactaaaagc cgtccttgat ggtgatatca atccaggtcg cgtctttact
    47221 tcaagttata aactggaaga tatcgaccaa gcctataaag atatggatga acgtaagaca
    47281 attaagtcta tgattgtaat cgaataaaaa acgaatagga gttttagaac tctattcgtt
    47341 ttttatgtta tcctattctt gatttagggt actttctctt aatgtcagtc tggttcccag
    47401 catggtcagg ctaggaattt tgcgaccgtg gaggacttcc ttgttaagaa tatccatacc
    47461 tgctcgcccc atttcttcag tataaactgt aatactagag aggggaggat agacctgttt
    47521 ggtcagactg gtgtcgttga atgaaatgag gctgacgcga tctggcaggc tgattccagc
    47581 ttcttggagg gcacggaggg caccgatagc taaactatcg ctggctgcga aaaatgctgg
    47641 cggaagttgg tctcccaagc tctgaatggc ctccttcatt aagtcatagc cagactgggc
    47701 agtaaatctt ccttgaaaga ccagttcatc atgatagatt cccctcgctt gactgtagtt
    47761 tttgaagttt tctagacgct tgtcctgaat gatttcttct tggtctgttg tttcctcaag
    47821 ccctgttaga atcccgatgc ggtccatccc ttgactgagg aaataatcga caacctgttt
    47881 catagcagtg taaaaatccg tgataataca ggtatgtccc agggaaagtg tatcgctgtc
    47941 tagaaataca agaggctttt ggtattcttc aaaggcagaa atctgagctc ggctaaattt
    48001 tccgatgcag agaatcccaa tcacttcctc gcttagggta aaaggatggt cattaaaata
    48061 gcgcaagata tcgtagtcca actcttgggc tcttttttct attcctaggc gaatctggta
    48121 gtagtagagg tcgtctagct ccccttgttc gctgacccat tgaataatgg caatcttttg
    48181 cttgggttta tgggactcgc ctgtcttgag gtgcttggtg tagcccagct cttcagcaac
    48241 ggttaaaata cggtgtctgg tttcttctgt aacagatagg ctctggtcgc ggttgaggac
    48301 acgggatacg gtcgcgatag agacagaggc tagctgtgca atgtctttta aggtagccat
    48361 aaatcctcct tgattaggtt agtatatcat atttttcttc tttttactga tgttttacta
    48421 aaattttagt aaaaaggatt gaccttggga aattccttgg atacaataga agaaaacgat
    48481 tacacgttaa gatggcttaa cggacagtca aaggagaatt catatggcac aacatcttac
    48541 tactgaagtc cttcgcaaag actttcttgc tgtttttggt caagaagcag accaaacctt
    48601 cttttcacca ggtcgtatta atttgattgg tgaacacaca gactacaacg gtgggcacgt
    48661 ttttcctgct gctatttcct tgggaactta cggtgcagct cgtaagcgtg acgaccaagt
    48721 cttgcgtttc tactcagcta actttgagga caagggcatt atcgaagtgc ctctcgctga
    48781 cctcaagttt gaaaaagagc acaactggac caattatcca aaaggggttc ttcatttctt
    48841 gcaagaagct gggcacgtga ttgacaaagg ttttgatttt tatgtttatg ggaatatccc
    48901 aaatggtgct ggcttgtctt cttcagcatc cttggagctc ttgacaggag tcgtggcaga
    48961 gcatctcttt gatttaaaat tagagcgtct cgatttggtt aaaatcggca aacaaacaga
    49021 aaacaacttt atcggagtca actctggtat catggaccag tttgctattg gtatgggggc
    49081 tgatcaacgt gctatttacc ttgataccaa caccttagag tatgatttgg tgccacttga
    49141 tttgaaggac aatgtcgttg ttatcatgaa caccaacaaa cgccgtgaat tggcggactc
    49201 taaatacaat gaacgtcgtg ctgagtgtga aaaagcagtg gaagaattgc aagttgcctt
    49261 ggatattcag accctgggtg aattggacga gtgggccgtt gaccaatata gctatctaat
    49321 taaagatgaa aatcgtttga aacgtgctcg ccatgctgtg cttgaaaacc aacgtaccct
    49381 taaagctcaa gcagcccttc aagcaggtga tttggaaaca tttggtcgtt tgatgaatgc
    49441 gtcacacgtt tctctggagc atgattatga agtaactggt ttggaattag atacccttgt
    49501 tcacacagct tgggcacaag aaggagttct cggtgctcgt atgacagggg ctggttttgg
    49561 tggctgtgcc attgccttgg ttcaaaaaga tactgttgag gcctttaagg aagctgtagg
    49621 caaacactac gaggaagtag ttggatacgc tccaagcttc tatatcgctg aagttgcagg
    49681 tggcactcgc gtccttgact agtcaaaagg aggctctata gtgaccttag tagataaatt
    49741 tgtaacacat gtcatttctg aaagttcatt tgaggaaatg gatcgaatct acctgaccaa
    49801 tcgtgttttg gcacgagtgg gagaaggtgt tttggaagtt gagaccaatc tggataaatt
    49861 gattgacctc aaggcccagc tagttgagga agccgttcga ttagagacga ttgaggatag
    49921 tcagactgcg cgtgaaatcc ttggtactga actgatggac ttggtgactc cttgtccgag
    49981 tcaggtcaat cgtgacttct gggaagccta tgcctactct cctgagcaag cgatagagga
    50041 tttttatcaa ctcagtcaga aaaatgacta catcaaactc aaggccattg ctaaaaatat
    50101 cgcttatcgt gttccatctg attatggaga acttgaaatt accatcaatc tctctaagcc
    50161 tgaaaaggat ccgaaagaga ttgcggtagc caagttggtg caagctagta attatcctca
    50221 gtgtcaactt tgtttagaga atgagggtta ccatggtcgg gtcaactacc cagctcgcag
    50281 caaccaccgg attatccgtt ttgaaatggt tggtcaggaa tgggggttcc agtattcgcc
    50341 ctatgcttac tttaatgagc attgtatctt tttagatggc cagcatcgtc ccatggccat
    50401 tagtcgtcag agttttgaac gtctgttggc tatcgtagag cagtttccag gatattttgc
    50461 tggatctaat gccgacctgc cgattgtggg gggctctatt ctaactcacg atcattatca
    50521 gggaggccgt cacgtatttc ctatggaatt ggctcccttg caaaagacct tccgatttgc
    50581 tggttttgag caggtcaagg ctggaattat caagtggcca atgtctgtcc tacgcttgac
    50641 ttcggattcc aaagaggatt tgattaactt agctgataag attttccagg aatggcgtca
    50701 gtattcagat tctagtgtgc agattttagc agagacagac gggacaccgc atcacaccat
    50761 cacaccaata gcgcgtaagc gtgatggaca gtttgagttg gacttggtct tgcgagacaa
    50821 tcagacttca gcagagcatc ctgatggcat ctatcatccc cacaaggatg tccaacatat
    50881 caagaaggaa aatatcggct tgattgaggt catgggcttg gcaatcttgc caccacgtct
    50941 gaaagaagaa gtggagcaag tcgcgagcta tcttgtagga gaagctgtta cagttgccga
    51001 ttatcatcag gagtgggcag accaactcaa atcccaacat ccagacctaa cggataaaga
    51061 aaaagccctt gcaatcgtca aggactctgt gggtgctatc tttgcgcgtg tacttgagga
    51121 tgcaggagtc tacaagcaga cagaacaagg gcagacagcc tttatgcgct ttgtggaaca
    51181 tgtcggaatt ttactagact aggagctttc tccttgcaca gtcctataaa aagtagtatc
    51241 atagtgtcgt ttgaaatatc aggaggaaac gatgaaagac tttcattttg acgctatatc
    51301 tgcctttgaa aattacgaaa ttgaaaaaat gagagatggt cacgttgtgg tgacgaccaa
    51361 agtagtgaac tcgtcgctca actactatgg caatgcccat ggtggctatc tattcacgct
    51421 atgtgaccaa atcagtggtt tggtggttat ctcgctggga cttgatgggg tgacactcca
    51481 atcctctatc aactacctta aggcaggaaa actcgacgat gtattgacca ttaaaggaga
    51541 atgtgtccat caaggccgta caacctgtgt gatggatgtg gatatcacca atcaagaggg
    51601 cagaaatgtc tgtaaagcaa cctttaccat gtttgtcaca ggccagcggt cagaagaaag
    51661 acaggtaagg atatagataa acaaaaaaga ggcttacacc tcttttttag tttcagataa
    51721 atcaatcaat ttctttaata aacttagcaa tttcctttac aatctcagta accaaagcat
    51781 ttcccataaa aaacattctc attttatcag atacctctac aatagaatca tcagataatt
    51841 tctttttagc tgtccaatca tctgggaaat cctgaagtcg ttcagcttca atgggggtaa
    51901 ttaatctata tttattattt acaaataata aatgtgtaga acgattaacc gttccttcgg
    51961 atgtaagcat tgttcgtcca ggaaggttta aatcatcata aggtgacatt cccccttctg
    52021 aatagatgta ttgatgtccg tcagaggatg ttcgttcaat ttttttaggt cctctaagat
    52081 attgaaattt ttccaatttt gactgatcag ttaaaaaata tttttcaggt acttcagact
    52141 catcttgaag aatttctcct agagtgatag gattaccatt gtaattaggt tctgtatcaa
    52201 tagaatagta ctttccacgc ctcataattc ctgtattcca aacagtacca gtaaaagtat
    52261 ctgaaacttc aactatatca tttggtaact cataaaacac atgacgattt ttaactgcta
    52321 taggtttaat aggaaattgt tttgcaaata gaccttgatt gaaaatatag tcatcatacc
    52381 tattatcttc aaagatttct tcattttttt cgtaaagatt gtcaattttt tgggcaaaga
    52441 ctgtatcatt tcgatatacg aaaaagaata cacgacgtct tcgttggctt cttccatact
    52501 cagcagcatt aataactctc cactcaacag aatatccgag attattaaaa gctgtgagca
    52561 tgattgcaaa atcacgtcct ctttgttttg agggcgcttt taaaagccta tctacatttt
    52621 ctagaattaa gtatttgggt ttaatgattt ccgttgctct aatgatttcc caaaataaaa
    52681 ctcctttttt tccttcaata cctttctcat tttttttact acgagcaaca gagtaatctt
    52741 ggcaaggaaa accaccgaca atcatatcgg catccatttt ggaaaattgt tcgtctgtta
    52801 tatcagagat actaatattg atgtttttgc tattggggaa gtgataatca tagacctcaa
    52861 atgcgtcttg agatttacga gatggttccc attggtttgc ccattttgtt ttaaagagtt
    52921 gcttatcggc attttctaat cctatgcgaa acccgccaac tccagcaaat agttcgagaa
    52981 ctttcatctt ttctccttca tttttttaca gtttaatcca ttatatacta ttttcaatga
    53041 ttgatcaagt attaatgttt gctttagttt aatttgatat aatataaaag ataggagaa
//
LOCUS       DAVKGU010000005        43826 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKGU010000005 DAVKGU010000000
VERSION     DAVKGU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.005"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            140..1549
                     /locus_tag="VW077_000257"
     CDS             140..1549
                     /locus_tag="VW077_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9454350.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKKEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKRS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1567..2862
                     /locus_tag="VW077_000258"
     CDS             1567..2862
                     /locus_tag="VW077_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9454351.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2867..3175
                     /locus_tag="VW077_000259"
     CDS             2867..3175
                     /locus_tag="VW077_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9454352.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALEWIGEIR
                     "
     gene            3172..3480
                     /locus_tag="VW077_000260"
     CDS             3172..3480
                     /locus_tag="VW077_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9454353.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(4347..6998)
                     /gene="adhE"
                     /locus_tag="VW077_000261"
                     /gene_synonym="adhC"
     CDS             complement(4347..6998)
                     /gene="adhE"
                     /locus_tag="VW077_000261"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9454354.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(7298..7708)
                     /locus_tag="VW077_000262"
     CDS             complement(7298..7708)
                     /locus_tag="VW077_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454355.1"
                     /translation="MENLTNFYEKYRVYLTRPRLEILAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            complement(7710..8138)
                     /locus_tag="VW077_000263"
     CDS             complement(7710..8138)
                     /locus_tag="VW077_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9454356.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQQYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            complement(8185..8484)
                     /gene="yajC"
                     /locus_tag="VW077_000264"
     CDS             complement(8185..8484)
                     /gene="yajC"
                     /locus_tag="VW077_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9454357.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            complement(8601..10577)
                     /gene="tkt"
                     /locus_tag="VW077_000265"
     CDS             complement(8601..10577)
                     /gene="tkt"
                     /locus_tag="VW077_000265"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9454358.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            complement(10691..11782)
                     /gene="ulaG"
                     /locus_tag="VW077_000266"
     CDS             complement(10691..11782)
                     /gene="ulaG"
                     /locus_tag="VW077_000266"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9454359.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            complement(11957..13567)
                     /locus_tag="VW077_000267"
     CDS             complement(11957..13567)
                     /locus_tag="VW077_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9454360.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKSLLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKF"
     gene            complement(13746..14450)
                     /locus_tag="VW077_000268"
     CDS             complement(13746..14450)
                     /locus_tag="VW077_000268"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9454361.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            complement(14452..15315)
                     /locus_tag="VW077_000269"
     CDS             complement(14452..15315)
                     /locus_tag="VW077_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9454362.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            complement(15319..15984)
                     /locus_tag="VW077_000270"
     CDS             complement(15319..15984)
                     /locus_tag="VW077_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9454363.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRRITEAADPAGAARAFKDEIKR
                     IWG"
     gene            complement(16001..16486)
                     /locus_tag="VW077_000271"
     CDS             complement(16001..16486)
                     /locus_tag="VW077_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9454364.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            complement(16566..16844)
                     /locus_tag="VW077_000272"
     CDS             complement(16566..16844)
                     /locus_tag="VW077_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9454365.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQAL"
     gene            complement(16867..18324)
                     /locus_tag="VW077_000273"
     CDS             complement(16867..18324)
                     /locus_tag="VW077_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9454366.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            complement(18455..19078)
                     /locus_tag="VW077_000274"
     CDS             complement(18455..19078)
                     /locus_tag="VW077_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9454367.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKILDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            complement(19129..20115)
                     /locus_tag="VW077_000275"
     CDS             complement(19129..20115)
                     /locus_tag="VW077_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9454368.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            complement(20134..20958)
                     /locus_tag="VW077_000276"
     CDS             complement(20134..20958)
                     /locus_tag="VW077_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9454369.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            complement(20933..21304)
                     /gene="rnpA"
                     /locus_tag="VW077_000277"
     CDS             complement(20933..21304)
                     /gene="rnpA"
                     /locus_tag="VW077_000277"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9454370.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            complement(21453..22643)
                     /locus_tag="VW077_000278"
     CDS             complement(21453..22643)
                     /locus_tag="VW077_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9454371.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            complement(22694..23647)
                     /locus_tag="VW077_000279"
     CDS             complement(22694..23647)
                     /locus_tag="VW077_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9454372.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            complement(23708..24300)
                     /locus_tag="VW077_000280"
                     /pseudo
     CDS             complement(23708..24300)
                     /locus_tag="VW077_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(24403..24849)
                     /locus_tag="VW077_000281"
     CDS             complement(24403..24849)
                     /locus_tag="VW077_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9454373.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQVFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFSSVK
                     IKEEKTDKKEEVVTDSSEKAEKKNQKRSLKRKRIHSQFNYNALNPE"
     gene            complement(24827..25288)
                     /locus_tag="VW077_000282"
     CDS             complement(24827..25288)
                     /locus_tag="VW077_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9454374.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(25251..25511)
                     /locus_tag="VW077_000283"
     CDS             complement(25251..25511)
                     /locus_tag="VW077_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454375.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(25516..25995)
                     /locus_tag="VW077_000284"
     CDS             complement(25516..25995)
                     /locus_tag="VW077_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9454376.1"
                     /translation="MDASRKNRLKPIKNTMINKILVKSLKIKAFTMLESLLVLGLVSI
                     LALGLSGSVQSTFAAVEEQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQK
                     LPVPKGIQAPSGQSITFDRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(25913..26236)
                     /gene="comGC"
                     /locus_tag="VW077_000285"
     CDS             complement(25913..26236)
                     /gene="comGC"
                     /locus_tag="VW077_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU9454377.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQDDGRITEEQAKAYKEYHDKQNTS
                     QIVKD"
     gene            complement(26238..27254)
                     /locus_tag="VW077_000286"
     CDS             complement(26238..27254)
                     /locus_tag="VW077_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9454378.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMKSLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(27202..28143)
                     /locus_tag="VW077_000287"
     CDS             complement(27202..28143)
                     /locus_tag="VW077_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9454379.1"
                     /translation="MVQEIAQEIIRSAREKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYGNLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(28219..28584)
                     /locus_tag="VW077_000288"
     CDS             complement(28219..28584)
                     /locus_tag="VW077_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9454380.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(28735..29793)
                     /locus_tag="VW077_000289"
     CDS             complement(28735..29793)
                     /locus_tag="VW077_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9454381.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(29956..31107)
                     /gene="nagA"
                     /locus_tag="VW077_000290"
     CDS             complement(29956..31107)
                     /gene="nagA"
                     /locus_tag="VW077_000290"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9454382.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(31260..33077)
                     /locus_tag="VW077_000291"
     CDS             complement(31260..33077)
                     /locus_tag="VW077_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9454383.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(33178..34320)
                     /gene="tgt"
                     /locus_tag="VW077_000292"
     CDS             complement(33178..34320)
                     /gene="tgt"
                     /locus_tag="VW077_000292"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9454384.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLSSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            34450..35307
                     /locus_tag="VW077_000293"
     CDS             34450..35307
                     /locus_tag="VW077_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9454385.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(35334..35951)
                     /locus_tag="VW077_000294"
                     /pseudo
     CDS             complement(35334..35951)
                     /locus_tag="VW077_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(36058..36426)
                     /locus_tag="VW077_000295"
     CDS             complement(36058..36426)
                     /locus_tag="VW077_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9454386.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(36437..36775)
                     /locus_tag="VW077_000296"
     CDS             complement(36437..36775)
                     /locus_tag="VW077_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9454387.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVETAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            complement(37184..38326)
                     /locus_tag="VW077_000297"
     CDS             complement(37184..38326)
                     /locus_tag="VW077_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9454388.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDVQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAKKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVAERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(38492..39112)
                     /locus_tag="VW077_000298"
     CDS             complement(38492..39112)
                     /locus_tag="VW077_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9454389.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(39116..40396)
                     /locus_tag="VW077_000299"
     CDS             complement(39116..40396)
                     /locus_tag="VW077_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9454390.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(40457..41941)
                     /locus_tag="VW077_000300"
     CDS             complement(40457..41941)
                     /locus_tag="VW077_000300"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9454391.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(42017..43294)
                     /locus_tag="VW077_000301"
     CDS             complement(42017..43294)
                     /locus_tag="VW077_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9454392.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VEERFSIYMEAIQKKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(43401..43484)
                     /locus_tag="VW077_000302"
     tRNA            complement(43401..43484)
                     /locus_tag="VW077_000302"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43448..43450),aa:Leu,seq:caa)
     gene            complement(43494..43565)
                     /locus_tag="VW077_000303"
     tRNA            complement(43494..43565)
                     /locus_tag="VW077_000303"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43531..43533),aa:Gln,seq:ttg)
     gene            complement(43576..43648)
                     /locus_tag="VW077_000304"
     tRNA            complement(43576..43648)
                     /locus_tag="VW077_000304"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43613..43615),aa:His,seq:gtg)
     gene            complement(43663..43733)
                     /locus_tag="VW077_000305"
     tRNA            complement(43663..43733)
                     /locus_tag="VW077_000305"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43699..43701),aa:Trp,seq:cca)
     gene            complement(43738..43818)
                     /locus_tag="VW077_000306"
     tRNA            complement(43738..43818)
                     /locus_tag="VW077_000306"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43782..43784),aa:Tyr,seq:gta)
BASE COUNT        13007 a         9603 c         7868 g        13348 t
ORIGIN      
        1 taatattcta atagtagtat aacaaagttc aagaaatgag gcaaacacaa gatatgtatc
       61 aacaattttt aaaaatgtct agacaaattt taaaattatg ctatacttta tgtatagaat
      121 aatagaaagg aggttgagca tgaaaaaaaa gttagtattt cctaatctgt tttggtgggg
      181 agctgcttct agcggacctc agacagaagg tcaatatgga aaagtacatg aaaatgtgat
      241 ggactactgg ttcaaaacgc atccagaaga ttttttcgat aatgtcggac ctcttgtagc
      301 cagtaacttt tttcatactt acaccgaaga tttccacttg atgaaggaaa ttggagttaa
      361 ttctttccgc acttccatcc aatggagtcg actcatcaag aatttagaga caggtgagcc
      421 tgatccaaaa ggtattgctt tctacaatgc catcattgaa gaagctaaaa agaaccagat
      481 ggatcttgtg atgaatttac atcattttga tttaccagtg gaacttcttc aaaaatacgg
      541 tggttgggaa agcaaacatg tagtggagtt attcgtgaag tttgccaaga ctgctttcac
      601 atgctttgga gataaggttc attactggac aactttcaat gagccaatgg tcattccaga
      661 agcaggatac ttatatgctt tccattatcc aaatctaaaa ggaaagggaa aagaggccgt
      721 acaagtcatc tataatctaa accttgctag tgcaaaagtg attcaactat atcgctcatt
      781 aggacttgat ggaaagattg ggattatttt aaacttgaca cctgcttatc caagaagtaa
      841 ttctccagaa gacttagaag caagtcgatt tacagatgac ttctttaaca aagtcttctt
      901 gaatccagct gttaaaggaa ctttcccaga aagattggta aaacagctag agagagatgg
      961 cgtgttatgg agtcataccg aaaaagagct tcaactgatg aaatcaaata cggttgattt
     1021 tcttggagta aactactacc atccaaaacg tgttcaagca caagcaaatc ctgaggaata
     1081 tcagacgccc tggatgccag accaatactt caaagagtat gaatggccgg agcgtcgcat
     1141 gaatccatat cgtggttggg aaatttttcc gaaagccatt tatgatattg ctatgattgt
     1201 gaagaaagaa tatggtaata ttccatggtt tatcagtgaa aacggaatgg gtgttgaaaa
     1261 cgaagcacgg tttatcggtg aaaatggagt tatcgatgac gtgtatcgta ttgaatttta
     1321 tgaagaacat ttaagatggc tacataaagc cattgaagag ggaagtcact gttttggata
     1381 ccacgcttgg accgcatttg attgctggtc ttggaataat gcatataaga atcgttacgg
     1441 atttatctcc gttgatttag aaacgcaaaa gagaaccatc aagagatcag gaagatggta
     1501 tcgcaaagta agtgacaata acggttttga agtagaaatt gaggagtaaa gagaggagat
     1561 attcagatga aaaactcaaa atttatagat caatttgcca cctttgctgg taaactaggg
     1621 aaccaaattc atttaaaaac cctaagagat gcattcgtaa cagtaatgcc attatatatt
     1681 ttggcaggtt taatcgttct tttgaacaac acggtattta agtggatttt ccaaggggat
     1741 acattaacaa gattccaata ttggggaata acaattgcaa acggtacttt aagtatttca
     1801 ggtatgatta ttgctgtaat ggttggttat ttcttagcta aaaacagaga tttcgaaaac
     1861 ccgttagcag catcaatgct atcattagtt tctttaattg tgatgatgcc aaatacagtt
     1921 tctgtagttc ctgacggagc aaaagatgcg gtaaacattt caggtgttct ttcattcaac
     1981 aacacaggta caggcgcaat gttcgccggg gttatcgtag cgattattgc aacagaatta
     2041 ttcattgaat tatcaaacgt taaagcttta caaatgaacc ttggtgaaaa tattccacca
     2101 gctgttagta gatcatttag cgtattactt ccagtcatga ccgtcatctc cttatttggg
     2161 gttgtttcag cattattatt caatataact ggaatgaact taatctcaat cattacaatc
     2221 tttattcaag aaccaattcg tcatattggt acaagcttaa tcggggtcat tattatttac
     2281 tctttaggaa atatgttatg gctatttggt attcaccaag cagttattta cagtgccatc
     2341 ctagaaccat tactattaat taacattact gaaaacatca ctgcagcaaa taatggacaa
     2401 gccattccac acatcatcaa cttatcacaa atacaaacat tcgctttaat gggtggtagt
     2461 ggatctacat tatgtttatt aatagcaaca ttcttagtaa gtcgcaatgc tgtttctaaa
     2521 aacgtggcta aattatcttt tggacctggt atcttcaata tcaatgaacc agtattattc
     2581 ggttacccaa tcgtttataa catttcatta gctattccat ttatcacagt tccagtactt
     2641 ggtattttaa tcagctactt agcaacagtt acaggattca tgagtcctgc atttatacaa
     2701 gttccttgga ctacaccagt attcttaaat gcatggttag caacagcagg ggacgtgaga
     2761 gcagttctag ttcaattcat catctttgca cttggagttc ttctatacat tccatttatc
     2821 aaagttaatg acaaagttgt tgaacaagaa atggaaggtt aatattatga aacaaatttt
     2881 attagtatgt aatgcgggaa tgtcgacaag tatgcttgtt aaaaaaatgc aacaaagtgc
     2941 gactgaacgt gggattgaaa tttctatcca agcaaaatca atgacagaag ctaaaaaaaa
     3001 tatccatgaa gcagatgtca ttctaattgg gccacaaatt cgttatgagt tacttgctgt
     3061 gaaagaaatt gcaggaaata tccctgtaga taccattgat atgcgggatt acggaatgat
     3121 gaatggagca aaagttctgg aacaagcact tgaatggata ggagaaataa gatgacagaa
     3181 gaaatggaat atatctgttt tcagctcatt gccaatagtg gagcagccaa atcatccttt
     3241 attgaagcca tccaactagc taaggcagga aatttaaaag aagcgaaaat taaagttgaa
     3301 gaagcggaag actccttagt agaagcgcac aagattcact ccaatctcat acaaaaagaa
     3361 gctactggag aaaaaatagg attctccttg ctatttatgc atgcagaaga ccaaatggct
     3421 tcaacagaga ttattcagtt gctatctaaa gaattcatcg atctctatca aaataaatag
     3481 gaacactgtc gttcccatct gtctttatta atgacgacta cattgtagga acctccaaaa
     3541 gttggacaaa atgattgaaa ggattggatc ctgcattaca caagatccaa tccttttctt
     3601 ttactctagt ttttataata tgttgaatct agaatagtcc actgcagttt ctaaaaaatt
     3661 gctggaaata gatgttaagc tccaatttat ttgtttatat cttatttcag tttactatac
     3721 tagcttatta ttctagtgaa aaattgaatg agccactatt tatttaatat gtatgatgag
     3781 ggaatgactc aaaaacttct tgtcgatata agaatatctg agatagtatt ttaaaacgaa
     3841 aggactataa aagctcattt ccttcgcact tattttatag agatctatca ttatactctt
     3901 tagaaatcaa aaagcaacac accttgtcat cgttttatcg taaatctgtt aatgaggggg
     3961 ctagtcttat ctgcaatctc aaatcagtgt tttgagctga atggcactcg ttttctagtt
     4021 tgctctttga ttttaattgg gccttagtaa atactactct taaattaaac tatagtgaat
     4081 tgaaataaga tgtaaacaac tctatcagga aagtaaaatt aatttctata aatgttttag
     4141 aagcagatgt gtactattct agtttcaatc tgctatatat tttgtttcta ttctagtttt
     4201 tcaaaaaggg caaaaaggcc tcaacatcat aaaaaggcat cctatcaggt attcaaaaac
     4261 cttgatagga tgccttttat catagaaaaa ttactggatc tttccctcaa attgagtttt
     4321 tgtcttgctt ctagactgat aaacaattat ttacggcgtc ctggtctttc tttgtagcca
     4381 tagtatgcat cttcgatgat ttcttgcata tggtctacca ttggaagacg tgggttagct
     4441 ggtgaacatt ggtcttcata agccaggaag gctaattcac gagaatgttc tttccattct
     4501 ttttcgtcaa ttccttggtc tctaaaattc atttggatcc caatacgttc accgagttcg
     4561 tagacagctt ttgcgtaaga ttcaacccct tcttctggag tagaagctgg aagtccaagc
     4621 atgcgtgcga tatcttggta tttttcatct gcacggtagt agttgtactt aggccatgtt
     4681 gctgtcttag ctggacgtgt accgttgtaa cggataacgt acggaagcaa gatagcattt
     4741 gtacgaccgt ggattgtgtg gaattgcgca ccaatcttat gggccattga gtgagaaata
     4801 cctaagaagg cattggcaaa ggccatacca gcgattgttg aagcgttatg cattttctca
     4861 cgtgagtgga agtctgcatt cttaactgag ctttcgagat tttcaaagac caatttaatg
     4921 gcttgaagtg ctaaaccatc agtgtagtca ctagccattt gtgatacata tgcttctgtt
     4981 gcgtgagtca atacgtccat accagtatca gcagcaacaa atcctggaac tgtcaatacc
     5041 aaagcaggat ctacgattgc cacagttggt gtcaatgagt agtcagcgat tgggtattta
     5101 cggttgtttg ctttatcaga gataacggca aatggtgtta cttcagatcc tgtaccagat
     5161 gtagttggaa tcgcgatgaa tttagtcttc ttaccaagca atgggaactt gaaggcacgt
     5221 ttacggatat ccatgaattt ttggacaagg tcacggaagt ccacttctgg ttgctcgtag
     5281 aagagccaca ttactttggc agcatccatt ggagacccac caccgagtgc gatgatggta
     5341 tctggtttga aggcacgcat aatctcagta ccacggttta cagttgtgat atctggatcc
     5401 ggttctacat ccgcaaagat ttggtaaaca accttattgc gacgaaggtc cagttgttcg
     5461 atgatacgat caaggaaacc aagctctacc atggcatggt cagtaacgat catgacacgt
     5521 tcaacgtcac gacatttttg aaggtattga attgaatcac gttcaaagta tgtttttgaa
     5581 ggaagtttca tccattgcat gttatttctc cgtcttccga cttttttgat attcaagagg
     5641 ttaatggcac taacgttatc cccaactgag ttgcgtccgt aagaaccaca tccaagtgtc
     5701 aatgatggca agaaggcatt gtaaacgtcc ccgataccac caaaagtaga aggtgagtta
     5761 cagataacac gaatagcttt aacagcttta ccaaattctt tagtcaattc ttcgtcagct
     5821 gtgtggatag ctgctgagtg tccaagaccg ttaaattcaa ccatttgacg agccttagta
     5881 ataccatctt cacggctttc agatttcaaa actgcaataa ctggtgacaa tttttcacga
     5941 gtcaatggct cattttcgcc aacttcttta cattctgcag caagaatgtt tgttccttct
     6001 ggaactgtaa atcctgcttg ttctgcaatc caagttgctg gtttaccaac gatgtcagcg
     6061 ttcaattttg caccagcaca gtttttgctg tttgctttga cgccgaagca gaactcttca
     6121 agaagagctt tttctttttt gtttacaaag taagtgtggt aagatttgaa ctctgctaca
     6181 aattcatcgt aaatttcttt atcaatgata actgcttgtt cagatgcaca gaccataccg
     6241 ttatcaaatg atttagacat gacgatatcg tgtgctgctt gacgaatgtt tgctgatttt
     6301 tcaacataag ctggaacgtt tccggcacct accccaagag ctggtttacc acatgaataa
     6361 gccgccttaa ccatggcatt accacctgtt gcaaggattg tcgcaacacc ttcgtggttc
     6421 ataagggcac ttgttgcttc catagatggt tgagtaatcc attgcacaca gttttcagga
     6481 gcaccagctg cgatagctgc atcgcggacg atacgagctg catgagcaga tgattcttgt
     6541 gctgatggat ggaaggcaaa gacgattggg ttacgtgtct tcaatgaaat caatgatttg
     6601 aagattgctg ttgatgttgg gtttgttgtt ggagtaatac cacaaacaac accaactggt
     6661 tcagcaataa gagtcaatcc tgttacatcg tcttcttcga taacgccaac tgtcttagtg
     6721 tgacgcatgt tgtttactac gtgttcacag gcaaacaagt tcttagttgc tttatcttca
     6781 aatacaccac gtcctgtttc ttcaaaggca tgtaaagcca attctccgtg ggcatccaaa
     6841 gctgctactg atgctttggc aacgatgtag tcaacttgct cttgatccaa tttacgcatt
     6901 tcttcaaggg caactagagc tttttgaacc aactcatcta cgtgtttttc agcaacgagt
     6961 ttcttttcct ctggtgtcac agttttttta tcagccatat tttcctccat agctttcaag
     7021 gatcgatatc ctttgttaat tttttcacaa gtttattata acgcattatt tcaactttgt
     7081 aaacagtttc ataaacattt cacaaagaat ttttaaactc ttgtgaaact tttctcattt
     7141 tcttttctta tcaggtttta tctttgaagt ttgtgaaatt tatttcaaat attttttatt
     7201 tcacaaactt gcttggaaga gggatttgga aagaaaggga tttacaggta agcgcttacc
     7261 ttgtaattct aataatacct cctttgataa ggaggcttta tttttgttga aaaacgcctt
     7321 gtttaaaagt tccttcataa acgacttctt gttctgttgt tagtttccct tttccttcag
     7381 cctgaccatt tacaaaatca ccttcgtagg tccagccttc tttagattga aaggtacctt
     7441 ttccgttgaa ggctccattg ttgaagccac ctgtatattg gtctccattt tggaaggtaa
     7501 tggtaccttg gccattcatt ttaccgcgga caagactgcc atcataaaca atcgttccat
     7561 tatcgagttt taagacacct tttcctggaa tatttagaaa aaagacgagt acagcacaga
     7621 aaacaatggt aactactgcc aaaatctcta aacgtggacg agtcagataa acacgatact
     7681 tttcgtaaaa attcgtaaga ttttccacct taactctcct tttctaaacg ttctaaccaa
     7741 gcttgacagc cctcttgaac acgtcgatag gtttcttcaa aatctcctgt ataccaaggg
     7801 tctggaacac tttcagatga aaatgagtaa atcttatctt gacagtctac tggacacatc
     7861 tgacgtaagt cagaaatatt tgaagcgtcc attccgataa tataatcaaa ggcttcaaaa
     7921 tcttccttac taatctgaag cgatgtcttg ttcttgtcat aaggaatctc atactgttga
     7981 aaaattccct gagtcccctt atgaatcgga ttgccatgtt cccaagagga agttgctcga
     8041 ctttggattt cgtagttatc tgtcattgat ttcataacaa actcggccat agggctacgg
     8101 caaatatttc ccagacagac aaagactaat tttttcatcc catttccttt cttttataga
     8161 aaaacgggag ttcgtgatcc cgtcttattt ttcaattgcg ccttctagtg aagctgtttc
     8221 tttcagcggt aatactgtct tgatagcagc tagttcaaaa gttaagtaaa ctccatctac
     8281 gtcaagaact attgttccct tctccgtatc tacttcatcg actgttccat aaagtccacc
     8341 gattgtaatc acttcatagc ctttttgtag tttatttaag ctttccatac gcttttgagc
     8401 ttgtttcttt tgagaacgtt gcataaagaa catcaaggcc atcataccta caagcatgat
     8461 taaaaaagta atatttggat tcattgtttt ctcctttgtc ttttacatag gactactata
     8521 tcaaatttta gctactttta caagattgta ccttactttt ctggtaagaa aaaccagagc
     8581 ttacgccctg atttttagga ttatttcaag tttcgaacaa tttttacaag attttctaca
     8641 gtaaagccat attctgccaa tacttttggt gctggggcag aggctccgaa ggtatcaata
     8701 cctagaacgg caccatcgag accaacatat ttgtaccagt tttgacttgc acccatttcg
     8761 actgcaacac gacggcggac tgcatttgga agaatctctt ccttgtaagc tgcatcttgt
     8821 ttatcaaaga catctgtaga tggcatgctg actacgcgga ttttttcgcc ttgactagcc
     8881 aattctttag cagctgagac agcaagatta acctctgaac ctgtcgcaat caagatggta
     8941 tcaaagtcgg ctgcattttc atatacaaca taagcacctt tagcaacctt gtcgaagtct
     9001 gttccatctt caacagtcaa gttttgacgt gtcaagacaa gggcagttgg tgttttctca
     9061 cttgtcactg caaggtacca agctgcttgc gtttcacgcg catctgctgg acggaaaaca
     9121 tttagatttg gcatagcacg aagacctgct aaatgctcaa ccggttcgtg agtcggacca
     9181 tcttccccaa ctgcgattga atcatgggta aagacataag tcacaggaag tccttgtaag
     9241 gctgacaagc ggacagctgc cttcacatag tcagagaaga cgaagaaagt tccaccgtat
     9301 acacgaagtc caccatgaag ggccatcccg ttcaagattg ttcccattgc aaattcacga
     9361 acaccaaact gaatgttacg attcaagcga ttggtatcgt cttgaagtcc atccgtcttg
     9421 atataagtca tgtttgagtg agcaaggtca gctgatccac ctaggaaggt tggtaattta
     9481 gctgccacaa cattcaaggc atcttgactt gaattacgag ttgcttgaga aaaaccattt
     9541 tctaaagctg ggaagtctgc tggagtcact tcgactggat cacgtccgtc gatgatggct
     9601 tctacttctg cagccagttc tggatgagct tctttataat ctgcaactaa tttagtccaa
     9661 gcttgataag ctgatgcgcc acggtctgca acatgttctt tgaaatcagc atatacttgt
     9721 tctggaattt caaatggttc gtagtcccaa ccgagggctt gacgagttga tgcagtttca
     9781 tctgctccaa gaggggcgcc gtgtacagca ttagttcctt gtttgtttgg agaaccgtat
     9841 ccaataaccg tcttcacttc aatcaaagat ggcttgcctg aagcttttgc tgtttcgata
     9901 gcagcatgga tggcttccaa gtctgttcca ttttcaacca aggcagtatg ccaaccgtag
     9961 gcattgtaac ggtcacgaac actttctgta aaggaatcct ttgtctcacc atccaagttg
    10021 atatcatttg aatcataaag aacaaccaac ttatcaagtt tttgcaaacc tgcgtatgaa
    10081 gctgcctcgc ttgagacacc ttccatcaag tctccgtctc cacagataac gtaagtatag
    10141 tggtcaaaga tattgtagcc ttcacggtta tatttggctg ccaagaaacg ttctgcttgg
    10201 gcaaaaccag tagcagttga aatcccttgc cctagaggac ctgtcgtagc atcaatccct
    10261 gccgtatgac caaattctgg gtgacctggt gtttttgaac cccattgacg gaaactctta
    10321 atctcatcca tgctgacatc ttcaaaacca gaaaggtgaa gaagagcata aaggagcatt
    10381 gaaccatgac ctgctgaaag aataaagcgg tcgcggttaa tccagtttgg ttgagctgga
    10441 ttgatatgaa gttgttttgt aaagaggctg taagccatcg gagccgctcc cataaccaca
    10501 cctggatgac ctgagttggc tttattaatg gcgtcaatac ctagaaaacg aattgcatta
    10561 acagatagat ttgacataga atttcctttc tatttgaggt gattattgaa tcacacattc
    10621 tattttacta ttatatgaaa attatccgta tcatgcaaca tacggataac ctccaaagaa
    10681 tatttttata ttatagcaaa gctttaaatt gaatgttaga gtcttgttca aaacaatcat
    10741 caaaaccacg tggatgatgg tattctacta agtgttgatc ttgaggataa gtgtacttac
    10801 cgccaacttc ccagataaat ggatggaaat cgtattgcaa gcgatctttt cgcattttcc
    10861 aaagttctag aatctcatta gtagaagcca tgaagttaga ccagatatca tagtgaactg
    10921 ggataatgac tttggtacgc agattttctg ccatacgaag aaggtcgata gatgtcattt
    10981 tgtcttggat acctaccgga ttttcaccat agttattcaa agcaacatca attttaaagt
    11041 ctttaccatg ttttgcaaaa tagtttgaga agtgagaatc tgcaccatga tagatggttc
    11101 cacctggtgt ttcaaagata tagttaacag ccttttgagc catttcttca tctgtaacag
    11161 ccaagccagc aagttcaccg cctgtctcat cagcaccgtt cactgggaga gttaccaagc
    11221 aagtacggtc aaatgattct actgcatgaa tcttcatatc ttttaattcg atagtgtcac
    11281 ctggtttaac aacgatgata cgttcttttg gaacccccca tccttcccag attcgtccac
    11341 agtggtaagg accgataaac ttaacatgct ctaacttagg attgttgaga attgctgcag
    11401 ctgtgtatgg gtcgatatga tcactgtgga agtgtgaaac taagtaatag tctagttcgt
    11461 tgatagcaaa tggatcgata accattggct gaacacgcaa gtttggttgc aatttacgaa
    11521 cacctgccat atttgccatt tggtgcccac gaaccatatc tttcactttt ttggttgatt
    11581 ttccacggtt tgaccaaagg tccatgacaa cgttagcacc agctggtgtc ttaatccaag
    11641 taccacagtt gcctagccac cacatggcaa agttgccttc aggtacgact tcttcttcga
    11701 tttcttcgtt caaccatgtt ccccactctg ggaaagtggc taaaatccat gactctcttg
    11761 taatttcttt gacgtttggc atatgttgcc tcctttggat ataattgtta tctttgagat
    11821 tagtatacca cccttcaaaa cactaaaaaa gaccaagaaa aacacaggtt tgtgttcatc
    11881 attggactgt ccttcaaata tcttccaaaa aagcttgaac tactatttct tgatttataa
    11941 gtgtttgaac ctcttcttag aacttactag ccaacttgct gtctacaata taagacgaaa
    12001 taagactaga aagattttca ctgaaacttt tgctcttatt acgaaatata ttgtgtttaa
    12061 gatagtctag cattttcaaa atatcttctg cttcaaggat aggattaacc cttaaaatcg
    12121 gaaatctgct atccaaatca tcattagtag taatcactac atcaacttgt gcaatatctt
    12181 ccacactctt aaattgttct gttgtaaata cagtgtcaat ttgctcattt ggaaaataaa
    12241 gtttgcattg tttcagcaaa agtctcgaaa ccgcaacacc ttcatcacaa acgagataaa
    12301 cttttttcat atttttttga gacgatggtg tatactttaa aaatcctcca atatgaatcg
    12361 tcaaataggc aatatcatcg tctgttagcc gaataaacca tgctccttct aaaatttccg
    12421 cagattttct agtgactaaa aataattctc catacttgga tcgaatttgt tttgttagag
    12481 gatttttaga aaaaataccg taagtctttc taaataacaa ggatttacag tggatcatca
    12541 aatttcgtaa caaatcatcc ttgttctcaa tctccattcg gatttgtgat tcaaaatacc
    12601 agataaattc ttctaaagca agttttaatt gccgaaaatc atcactttct gcatgaatat
    12661 ccaaatcttt acgataggag aggaggagaa cagctacaag agaaacttca agtcctgaca
    12721 aagaaatttc aaacttttga aacaaccgtt ctcctaattt cttagacacc tgatactcta
    12781 ttctttttct tatcaaagaa aattctttct ctatatcctg atgtctttct tgatactgtt
    12841 caacattatg acagcttaac agcaaataag gtagaacctg caacataaaa gttatttcat
    12901 gatggtttat tttcttccct aaatcttgtt caactaaagg aacctgttcc ttaaaaaatt
    12961 ggttcatttc aacagaaagc aaacactcat catctaacct ctctttcatc ttatcttcta
    13021 aaatagatac aaaagtggca ttttcttcca taaaaatatg atatagaagc gattgaagat
    13081 actgaatttt attaagaggg tgggcatgaa ggtggtatcc ctgtgacttg ctcacttgca
    13141 agatcacctg atattgttcc aaagttagtt gataacgaat actattcaaa tcattgagaa
    13201 cagtattcct agatacctct gttaactcta tcaatttttc aatcgtaata cgttctttag
    13261 aaatacctat ccatagtaac attatcatca tacgttcatg cgcactcatg atataatcgt
    13321 acgaatctac ttccgataat agtttacaac aagcatctct ctgctcttcc gttaagtgaa
    13381 taccaattcg tggaatacta atgatgtgaa gcgcctcgtc acccagagca gcattgattt
    13441 tgtcaatgtg ataataaatt ttccttcttg actctttcaa atctttcgaa atcgccataa
    13501 tcgttttgga cgtttcttga tccatcaaat attgaagcaa atcacaactt gccttatcca
    13561 gtaccatatt aaaaaatccc ccaaatctat tgatatcaca gcaattcttt ttattttacc
    13621 atatatatat caagcgctat ttctaaaaaa tcttttaaaa ttacttcaat tccaacttta
    13681 ctctcaataa aaatcaaatg taaaaagagg agctaaattt atctttttct cctccttcat
    13741 cgttcttact tttgaccata ataagcattt ggtccatgtt tacgttggta gtgtttttct
    13801 agtatgtact ggggagcagg tccaactctt ggattgattt gttctgtaaa gcgattcatc
    13861 tttgatactt cctctagtac gacagagtga taaacagcat tctctggatt tttgccccag
    13921 gtgaatggac cgtgattgcg tacaacaatt cctggtactt caaccgggtt aagtccgcga
    13981 tgttcaaact cttctacgat aaccaggcca gtatcttttt cataggccac ttctacttcg
    14041 tccttggtca aactacgggc gcaagggatt gaaccgtaga aataatctgc atgggttgtt
    14101 ccgtagaaag gaatatcacg acctgcctga gcccaagcaa cagcttctgt cgaatgggtg
    14161 tgaaccacac taccaatttc tgaccaagcc ttatataatt gcacatgagt tgggaggtcg
    14221 gaagatggtc ttaaatcccc ttctaggatc ttaccatcta gatcagtcac taccatgttt
    14281 tcaggtgtca attcgtcata atccacgcct gatggtttga taacaatgac accgagttcg
    14341 cgattaactt cagatacatt cccccaggta aatttgacaa gtccatgttt tggcaatgat
    14401 tgattggcat cacagactcg tttacgcata gcattgatta cttgattcat cttacatcaa
    14461 acctgctttc ttaatgagtg gatagagaaa agcttgcgcc tcttgaacgg ctgcgcgtgt
    14521 ttcttctact gtttcacaat tttcagacca catttcgatt aggaaaggtc cattataatt
    14581 ggtttccttt aaaatatcga aagcttcttc ccatttgaca caaccttgcc cgaaaggtac
    14641 atctcggaac tggccctttg aactttctgt cactgcataa gtatccttga gatggagagc
    14701 tgcgatggca tgatgaccaa gataaaactc actatagata tcattatgcc atgcagacac
    14761 attaccaata tctggatata caaagaggaa gggagagtca atctcttttt ctatagccaa
    14821 atatttttcg atgctattga tgaaaggatc atccataatt tcaatagcaa gtaccacctg
    14881 agcttcttca gcccagtcac aggcttttct caaatttttg ataaaacgtt ggcgtgtctg
    14941 gggtgacttt tcctcatagt aaacatcgta accagctaat tgaatcgtac gaactcccaa
    15001 gtcttgagct aattcgatac attttttcat gagttctaga gatttttcct ctagaactgg
    15061 atcttttgaa cccaatgggt agcgacgatg gcctgaaaaa cagatagaag gaatacgaac
    15121 accagtttca tagattgctt tgacaacttc caagcgttct tccttactcc agtcaagtct
    15181 tgctaaacgc tcgtcacgtt cgtcaataga catctcgaca aaatcaaagc ctaactcctt
    15241 ggcaaaattt aaacgttcta gccaagtaaa gtgtgttggg gttgcctttt cataaattcc
    15301 aattggacgt accatgattt acccccaaat tcgtttgatt tcatccttga aggcacgcgc
    15361 tgctcctgct ggatccgcag cctctgtaat tctacgacct gcgataaagg taaagacatc
    15421 aacaccttca aagagtttga gagtatctac atctagacca cctgttacag atacacggaa
    15481 gcccatgtca atgagttttt taaccttatt aaggtctttt tcaccccaag tttcaccagc
    15541 aagaagagca tcacgagatt ggtgataaat agcttgtgag atacctgcat ctagccaaag
    15601 ctgagcttgt tcaaaagtcc aatcgccata aagctcgatc tggatttcgc ctcgttctcc
    15661 tcgttcagtc ttgatagcct ttagagctgc ttccatagta gggatggttg cacaacagat
    15721 acaagtcatc cagtctgctc cacgaaccgc attattttta gcaactgttc caccagcatc
    15781 agcacatttt gtgtctgcca caataatctt atctgggaaa aggctacgca agacttcagc
    15841 cagttcactt ccaacttgaa gcaagcaaac agttccagct tcgataatat ctacttcctg
    15901 accaacagaa acagctgctt taatcgctcc ttgcaagtct gaatggtcta atgcaacttg
    15961 taaattaggt attctttttg tcatttcttt attcctcttt ctaactttcc aaatccaatc
    16021 cttcgagata agggctatcc ttagattctt caatcatagc caagacatct tctttagtct
    16081 ggcaagcctg taaacgtgca atagaatctt ctaattcaaa cagggcaata atttgtggaa
    16141 tggccacact tgtgtgaatt tttgaacttg ttgctgctag tgccaacaaa acagatacct
    16201 ctttcccatc tgaaaataca acaggatttt gtaaggtaat caatgaaaag gcatcacttt
    16261 gcacaccagc ttcaggtcta gcgtggggca tagccatacc tggcatcaag atatagtaag
    16321 gcccatactc ttcagtcgat tcaatgatag catcgtaata ctctggcaaa attgccccac
    16381 tttcaatcaa gggatctact gctaccttga ctgcttcttt ccaatcgtta gcctctaaac
    16441 ctagtcggat cgagtcgttg tcaattaaag cttgttttaa attcatatct gcctccttta
    16501 tttaaagagg gagaaggaca gaaacgataa ctctcagttt ctgtccagtc ccctccaacc
    16561 ttttactata gtgcttgact gagtttttcg gtgatttctt tatcatccat caagttatca
    16621 agaccaatta acttcccatt agttcgccct tccaattctt gaatcaaatg aagagaagcg
    16681 attacgatgt catatcctac tgctaaacct ttagcttcac cgacactgca tgaattgact
    16741 gtaaaatctg tttgattaag cttacggaga gcattttcaa ccttcatctt gataaccatt
    16801 gatgaaccca ttccatttcc gcacgctgct aatactttaa ccattttatt tctccttttc
    16861 tagatactaa gcttcttctt gaacttcacc gttgtaatat ttctctttat cttttgcttt
    16921 ggcaaattga agttgaggaa taacaagcaa gaagagacac acaagtacat aaccaacaat
    16981 accaaggtat ttgaagatat atccaaatcc aagccatggg aattcaaagt cgatatttcc
    17041 atggtagcca ccataagatg ccaaatcgag aagggccaca caaagagctc ctagagcaac
    17101 ttgaaggaca cctgatataa aggaaaggat aacagccgct ttccatccgc cgcgtttatc
    17161 agcgtagacc gcaatggctg cattgtcaaa gaacactggt acaaatcctg taataataag
    17221 aatcggattt ttaaagacga tgagcaagac aattgtaatc aattgaccaa tcaaaccaaa
    17281 ggtaaatcct gacaagacag catttggaga accaaatcca taagaagctg caacgtcaac
    17341 cgctgggaat gaacctggca acaatttgtt tgaaatacct tggaaagcgt ttgttaactc
    17401 agatacgaac attcggacac cttgcatcaa aacgaacaag taaactgaga aggtaaaggc
    17461 tgtttggata atgtacataa agaaatcttg tttagcagga ttgaatagag ttcctgaagt
    17521 gatgacttct ttattagaca taatgtctgg acccaagatt aaaagaatgg ccccgaagaa
    17581 tacgagcatc aaggtagcag atgcaacaac tgtatcgtgg aagattgaga ggaacttagg
    17641 taatttaaga ttgtctaaac tttcttcttt cttaccaaag cgtcctgcta ctttatctac
    17701 aaaccagatt gcaaattgct gttggtgacc aatcgcaaat ccgccaccac cagtcaagcg
    17761 ttgagttgcc tcaacagtca tatttgaact aactgcccag taaagtccac agatgatacc
    17821 aatcgctgct gtaccgtaag cattgcgcaa ttgtggtact aagaatagaa ccataagaga
    17881 tactgttgca gcttgttgta ccatgatgtg accagtaata aagagggttc ttaccttcgt
    17941 aatctttcga agagctacga gcaagatatt tattccgaaa ccaatcaata gagctgtagt
    18001 tgcagttcca acaaaatctg gaaactctgc tgcaattttg ttgtttgctg cagcaagtcc
    18061 aaagtaaggg tcgataaccg ctgcaccaat ttggaatttg tagttaagag ctgctaagat
    18121 tggacgaaag gttgtaacca aaccaccagc acccacgtta agcaacatat accctactgt
    18181 tgctttaaca aaccctgaaa aaacgtcatg ggcaggtttt ttcaaaagtg catatcctat
    18241 caacaccaat aaacctacga aaaatgcggg attctgcaaa atattgctag aaaaccaatt
    18301 tagaacactt gaaacgactt ccataattgt tctccttttg aattgttttt gtaagtttat
    18361 tatataatgc gaaagcgcaa acaaaaagac caagaattac acagcggact gttcactctt
    18421 ggactgtatg atttatgatg ttttacaaat tatcttattt tctcgtagct agataatagg
    18481 gaagaatttt agaataagac ttcaataagc cttctacgat ttcttcttca gacgaatttt
    18541 ctgtcatagg aacatctacc ttcactaaaa cttttcgaac ttcttgactt ctcaccttct
    18601 cgcgtaaagt acgacgcttt tcttcattcg cctcccaccg ttgactttga ccattagagt
    18661 aggttagata ataaatccct ttaacggttg gaatgtctaa aatcttggcc tgcttgccca
    18721 gtgtttgctc atccttctta cgttcgatga aactgacttc caaagaaatt ccaaagtcag
    18781 tagatgtccc atacaaacgt aaggccatca taggttctgt cacttgtccg tctctctgta
    18841 gataaaccca aaaatgtggt ctcaaacgct gggcctgatt catccactga ctagtctgtt
    18901 ggagttgcca ttctggatgg cttgcttgaa aggctttggc cagttctgta aaagcctttc
    18961 gggcctcttg tcctttgtgg cggaacgcca gcatcttctc tcgctctgct cccgctttat
    19021 caggactacg gtactgcaaa ccagaaaagt ctagatagtc tcttatctta ttcaacatca
    19081 gtttaacctc ctctagctag caaaaaatca ggtcaaccct gattttactt attctgtatc
    19141 tacaacgaca tagcgatttg gctcatcacc ttcagagtaa ctggtcacgc catccatacg
    19201 tgaaataata cggtggataa tcttgcgttc actattcgac attggatctg tttgatgact
    19261 acgtccttct tctaaaacac gaatcgccaa tttttgcgca taggtctgca agacttctgc
    19321 acggtgttca acataatcat tgacattaat agtgatatag aaggttcttg aatagcagtt
    19381 gtaaagataa ttttgagcca acagttgcaa ggccttcaag actttaccat ggtagccgat
    19441 aatacgacct ggttcgttag tatcaatttg caaattgatg ctacgacggt tataatcatt
    19501 tgaaagtgta gcctcaacat ccatgtcatc aataatcgtt tgaacataag cagtcacatc
    19561 cgtagccacc tgggcaatat cataactttg ctcgaccttc aagcccatct ctttcaagtc
    19621 ttgattttca gtttgatctt gctcagtttc aaggtcatca gcgcctgctt cttccctcat
    19681 cgcttcctcg atttgaagtt catttaaaat ctcaatgtga ccagtttctt ctaagatagt
    19741 gctggcatgt ctttcatgtt ttaagatttc agccttgact tcatcagaaa taccttgacc
    19801 ttcttcctct atttttttaa tagcatcaac cacatgacca aggtcaacgg tttcttcact
    19861 aacagtcttc acaggctcgt tcaaatcatt gatttttttc ggaacgcctt ttactacctg
    19921 ttgatttgct ttgacaacag tcgtttcact aatcgcttca atatccactt gggctggttt
    19981 tttaccaaat agaccaagaa agcctttttt ctccctagaa atgactttga tatgagcctt
    20041 cattcttgga atatctaatt ctttcaatcc tttctggatt gcttcttcaa cagttgaacc
    20101 tgtaaatact accattacca gattcctcct tatttatttc gttttctgag cctttttctt
    20161 ggcttttctt tttctatttt ccaaatcttt ttgtgcctgt actacggcct cgcgctctgc
    20221 gataatcttg aatggattat tcaagaaata ggtttgcaag acttgataag cattagacac
    20281 tgtccagtat agggcgactc cacctggcgc ataaactgca aagataaaaa tcaagactgg
    20341 aatcccatac atcatcgcag tcgtagcgcc atttcgctca gacaaagctt tgttggacaa
    20401 ccaagtactt aaaaaggtga atactgctgc taaaatcgga agaacaaggg ttgtatccac
    20461 actaccaagg ttaatccata agaaatgacc tgtctttaaa aagtcaactc ttgatagggc
    20521 ttggaacagg gccaaaataa ccggcatctg aatcaaaatc ggccaaagag agtctgactg
    20581 tctgacaccc atttctttaa atactttacg catttcctgc tctagtttgg ttctgctttc
    20641 catatctcga cctggatatt gttctcgaag cgccttaatg cgtggctgag cttcctgcat
    20701 tttcctagaa gccaccattt gcacctgaaa gactggcaag aggactgtac gaatcaagac
    20761 cgtaaagaga ataatcccca ctccgatact aatatcaaac gataaaaagc gaatgatttc
    20821 cgcaaagaag taaaccaatt tactccaaaa atcagccgat tcggctgtaa tatcgctagt
    20881 taccccatta gtcgcacagg ctgtcatgat taacagagac agtcctagca aactagtcaa
    20941 ctttagtttc tttttcactc ccatttcctt cccggtaaat ctttgataat tttaatacat
    21001 ggagtagatt tttctccatc tctgcgtatc ccaaggtttc gactcctttt cgagcaatga
    21061 caacaaagtc gacatcttct accagactcc cttttgcatt ctggataata tgccgaatcc
    21121 gtcgcttaat ttgatttcta gtgacggcat tccccagttt tttgctaact gatagaccta
    21181 ctcgaaaatg gtttttctgg ttttctaatt ggtagaccac aaatttgcga ttagcaaaac
    21241 ttgtcccctc cttgaaaatc gccttaaaat ctttctctct ttttacacga aagtttttct
    21301 tcaaaactca actccatcta ttaaattact actattatac catatttttc aaaaaagcca
    21361 atcatagcaa ggttttggac aatttcatca gaaaaagaag ctggaaaaat aactttccca
    21421 actccttgta ttgaatattt ttttagtttc acttattttt tcaagcgttc aacgtcacgg
    21481 gcaatgacta attcttcatc tgttggaata accaagacac ggattttagc tgcctctgtt
    21541 gagatgtctc ctgtaacgcc aaagacattc ttttcatcat caacatcaca accaaaccac
    21601 gaaatccctg agattacatc acgacggaaa ttctctgcat tttcaccgac acctgctgtg
    21661 aaaacaatgg catctgctcc atttagcact gcaaggtact gaccgatatg tttttggata
    21721 cggtcaacat acatttcata agccaagcta gcctcgtgat tcccttctgc tacagctgct
    21781 tctatatcgc gcatatcgct agaattagca gaaactccca aaagacctga ttcacggtta
    21841 agaacacgac tgatatcttc tggtgtgtta aaatcctctg tatattgcat taaataagga
    21901 atgatagctg gatcaatatc ccctgtacgc gttcccatca taataccacc aagaggagtg
    21961 aaccccatag aagtgtctac agatttgccg gctttcacag ctgtaattga gcctccgtta
    22021 ccaatatgac aggtaattaa cttcaagtct tctaatggac gtcccaagag ttttgcagct
    22081 tctcctgcta caaactggtg acttgtacca tgagcaccgt atttacgaac cttgttttct
    22141 gtgtaatatt ttgttggtag agggtagcga taagctttct ctggcatact tgtgtggaag
    22201 gaagtatcaa aaacaactac actggtaatg tctggcaaca attccttgaa ggcacgaaca
    22261 cctgctgcat tggccgggtt gtgtagagga gccaacaaac tcaactcttc aactttttct
    22321 aaaacatctc cctcaacaac tgttgattct ttgaaatatt ctccaccagc aacaacacga
    22381 tgtccaacac ctgtaatctc gtcataagcc ttgataatat cgaaacgaat caagtcatcc
    22441 aataaaattt taacggcttg tgtatgattt tcaatatcca aaatttgttg ttcagaacgg
    22501 ccgtcaaatt ttacagttga aattgaatct ttcaaaccga tacgttcaat caaacctttc
    22561 gccaatactt tttcttctgg cattaagtat aattgccatt tcaaacttga acttcctgca
    22621 ttgattgcaa ttgtttttgt cataacatga tacccctttt taagcgtttt caactattat
    22681 aacaaaatct attttatatt tcagtacctt gagtccattt ttgaaaattt tctttaaatt
    22741 tcattaaaac acttgcatct tgcaagctag caagtggata aacaaaaggc tctactgcta
    22801 tttcattttt cttctgtagg ataaaaatag tcttagattg tttagcatta gcaaagagat
    22861 tttcaggcag actaatcata gcaaccagac tcgcctcttc tttcagccat tcttttaaca
    22921 aatcactttg aggactggtc aacaaatcac tcggagctag aaaaatagcg tatccgtctg
    22981 acttgaggta cttaagccct tgttccatga gcaagtgatg ggcgtaagta tgttcttggc
    23041 tagaagcaac ttgatggcgc gacgcaacgg catcatcagg ataatagcca acaggcaagt
    23101 cactgatgac cacatcgctt tctttgagca tttgtggacg aacggcatct ccttggacaa
    23161 agccagcctg caaaccaatt acatctgcca tgctagctgc cagatcaatc agtaaatcat
    23221 ccacttccat tcccaagtaa tccacctttt tatcaagcga ggtcaagaaa gtagcgccta
    23281 gaattcccat cccagaaccc atttcgagga tagtaatttc ctcctcttta aacaactctt
    23341 ccacaataaa caccaaaagc aaagcaatag catccggtgt aaactggtga ttggcctgca
    23401 agggttctgt ttgcccagcc ttcatcaaga gaaactggta ggtcttgagc cattcttctt
    23461 tgcgtagtgc taaacgctta agggcctgat tgttgtcttt gacctggttt agctcagttt
    23521 caccatccag atagatgctg ttttgctcca ccaaggcgtc ataaaagttg gtcgccaaat
    23581 cactttggat gacttggaca ttctctagta aataggtata agcttgttca attttttcaa
    23641 aatccatatt cctatcttat caaatttccc ttcttttttc catccttata gaaaaatcac
    23701 tccctgacta ggcgagtgat ttttgggcta ctattaaaaa gaattctgag tgatttcctt
    23761 gaaacaggtc ttcagcgcta tagagaatct gactatgcac agatgaaaaa ccatgctcaa
    23821 ttagcttgtt ttccagttca gctaaatcaa atccatgatg attagcttct gtcttggtaa
    23881 aatcagcaat gatgagtttc ccatcttcct tcaaatgttg atgaaacagt gagagagccg
    23941 catccaaatc aggcatatga tgaagaaccc gactaacagc aaggcaatca aactctttct
    24001 ccaagggatt tttcggtaaa tcttgctcca aaaactggat attcttgatt gcttgctgct
    24061 ccactttcaa acgagcttgc tccaacattt tctcagaaat gtctacaaga gtgactgact
    24121 tagcctgctt ggctaggggt taggggcaag gctaatagac ccgccaccac cgaaatctaa
    24181 aatttctttg tctgatagaa gatcaatctg tttctcggct gcttgacata ccaagtttgc
    24241 gaggaagata tttttagggg aatcgaaagt ttctgctttg tggttaaaat catgtttcat
    24301 atctttagtt tagcacaaag tatagtaaac tgaaataaga tataaacaaa tgaattggag
    24361 attaacatcc atttccagca attttctaga aactacagtg gactattctg gattcaacgc
    24421 attatagttg aattgactat gaattctctt tcttttcagg cttctcttct gatttttttt
    24481 ctccgctttt tcgcttgaat cagtcactac ctcttccttt ttatccgttt tctcttcttt
    24541 gattttgact gaagaaaaca gaaactcata ttgactctta tccgtacgaa ccctcgtcac
    24601 taagccagtt ttcttgtttt gatagcttac ctgacctaga ttaaaaacct gttccccact
    24661 ttcttgctca accttatctt tggttcgttt agccatagca aaagcaacca atttttcttt
    24721 attcaaagca tagtcttgat agtgggcgac ttgtcggttc aagtaaaatt gcaacaaaag
    24781 actaaagata gctgccatgg tgactgcata gaggagaaca cctgccttaa ctttcttctt
    24841 tttccacacg atagatgaac tccctttcta agcctttttg aaactggaaa cgaaaatgaa
    24901 ccagttgatt gtcctctgta atctgagctg atttgaggcc ataaaccata ggctgatacc
    24961 cccgtccact agcatcggtt ttacgaaaat cgtctgactt tgacttaccg atggcgatgt
    25021 ccttgccatc ttgcttcatg tataggcgat tgccttctac tttttcgaac tgcgaacggt
    25081 ctaattctac ctcaagttgg tccacaaaca agagccactc cttttgctcg ctttgttgct
    25141 ggtagcgaac ttctgaaatg aggagctgac tcatagcttg aaaaaggagt aatcccccac
    25201 tgatgacaat gagggcaagc agggattcta acaaggtaaa agccttgacc ttatggctct
    25261 ttgattgcca acaactgttc tgaaccatgg tagacctcca atcctttttc actagaaaat
    25321 acctgaatct caactccgtt gatgcttacc tgattttgcc ccgtctgcaa ggccatctta
    25381 gctaccctca agacttcttc cttttgcaag atttttgctt cctcttgcct atttttttga
    25441 atttgtccca aaaggagggt cgcaatgctg gcaaagatag ctagagcgac tactgcttcc
    25501 agtaaaatca ctgccctaat tttttgtttc cttaatgcgt ttaatttttc catttcctag
    25561 atataattga tagcgaatcg ctcctttact ggtctgaaat tcaaccttag ccagggacga
    25621 attgccccca gcacggtcaa atgtaatact ttggcctgat ggggcctgaa ttcctttagg
    25681 gactggcaac ttttgactgc cattgctaat catctgccca tccaagttca gactagtctt
    25741 ttgttgacta gctacactgc gtttttgggt ttcccgatag agttcttcaa actccataaa
    25801 gaaaatctgt tcctctaccg ctgcaaaagt ggactggaca gagccggaca agcccaaggc
    25861 aaggatactc acaagtccca aaaccaagag actttccagc atggtaaagg ccttaatctt
    25921 taacgatttg actagtattt tgtttatcat ggtattcttt ataggcttta gcctgttctt
    25981 ccgtgatgcg tccatcgtct tgtaactttc ttaggctagc atcttcattc ttttccaagc
    26041 tataaagttc tgcctggctt tccaccacct taacaacagc tgcttttcct ttgtcattga
    26101 ctgcttcttt ttgcttggtc agattaggta caaagagcaa gaaaagcacg ctgataatca
    26161 gcaagaccac caacatttcc accaatgtaa aagctttaac cttagctttt ttcaagaatg
    26221 tcatcatttt tttcatttta aaaatttacc tccatatttt gatacatggg catgagcatt
    26281 gccgcataaa gtaaaacgat aatcagtgcc acaaaaataa aaaccagtgg ctgcaccaaa
    26341 ttcatggtgc ggttgactcg ggtaaaaaag gcttcccaag ttttttcagc atagatttcc
    26401 aactcactac ccagcttgga cttaacttcc ccatactcta tgatgagact caattccttc
    26461 ctaaagaaag gataggttcc tatcgtctga gaaaattcac ggccattttt cagggtttga
    26521 gccagatctt gaccgatttc tttaaagagc tgggaacctt gttcctgcat catttgaaaa
    26581 atctgcgtca gctccattcc ctgtgaaatc atattccccc attcacgtgc ataataggct
    26641 gtcaagtagg tctgcacaaa gattccaata aagggaaggc gtgctaagat agaaaagaca
    26701 ctcatcttag aacttctttt ataaaaagtt agtgctaaaa gggcaagcac ggaaacaagc
    26761 cctaccatgc ctagaaaaat ttgtggcaga ttaccgataa ttcgggtggc aatattgcta
    26821 ctatccagtt gtgggagcag gtaattccgt agccccagca taattaagag aagaaaaaac
    26881 agcaaaatca aaggataggt cgctacttca attaattttt tcttgacctt ggccagattg
    26941 tctagatact cttctatctt tcccaaactc aggtggagat taccatggac ttcagctagg
    27001 gataactgag tgacaatagc acttgaacat cccaaacttt tcatcatttc tgagaatgat
    27061 ttcccctgag acaagcccac acgcatctgg gtcacacact gcttgtccaa caaagcactc
    27121 ctatctaaaa aggagatagt ctccaccaga tgaaaaccgc tagaaaagag attgttaaat
    27181 agggtgatga tatttttttg cttagctgta gctaattttt tccgtctcag cctgaagact
    27241 tgtgatatgt ccatctttaa gaagctggtc aatttgctca ttccacttgg ctgcttggtg
    27301 ttcttgataa tctctgcttg caaagtcaac gattcctcct cccccgatta atctctggta
    27361 gcagactcct tgcagaacaa ctgccaattc ttcttcactc acacccaact ccagcagacg
    27421 ctcataaaca cctcggatac tcttggcgtg aatggttgaa aagactgtcg cacctgtcaa
    27481 actagctctg accactgcac gcgccgtctc gctgtcacga atttctccga taatcaagag
    27541 atctggtcga tgacgcaagg aaagtttgat tagatttcca taggttaggc cgattgcttc
    27601 gttcaactgc aactgaagca tgtcgtcctg cttgatttcg acaggatctt cgatggacat
    27661 aacttgctgt cctttaaaga gtgacttgga caattcatgc atcaaggtcg tcttaccact
    27721 cccaaccgga ccagcaaaaa gatagagtcc ccgttgcctg tactgcttgc ctaattcttc
    27781 aatatcctga aaccaaaaat gcaagtcctg ttcctcatcg tgcaacaaac ggataaccaa
    27841 actctcatgc ccccgataat cgcctacagt agataaacgt agagacgcca tcttctggtc
    27901 ataggcataa tcacaggaac ccagttgact acgtcttttt tctcccacat tcatacccgc
    27961 cacaaactta aagtgactga taacggctgc aaacttttca aaatcatagc taccaatttt
    28021 acagcgctcg tctcctaccc tcatatgaag ctcataggcg tctaacttag ggacaaaata
    28081 aatatcctgc gtcccttttt cccgagctga acgaatgatt tcttgtgcaa tttcttgaac
    28141 catacttacc tcctcaccta tactattcgc aaagagttag aaaaataaaa aaagcaactc
    28201 caaaaaagtt acttttctct attttaattt catacggcaa gaacgattcc tttcctgact
    28261 ggtttgtttt tcatagcctg agcgtagttt ttcgtctggg ataacctgct catcctgcaa
    28321 aagagccaaa gaatggtatt gttgtaaata ctcatcacat tcatcacacc agcgttggtc
    28381 ttccgggtcc caaaaaatgg tcatcaaatc gcttctactt ttataaagag gggattcttg
    28441 ttccaatcta aaccagcaag ttttgtagta tttgagagca tcataatact gatcaaattt
    28501 tctacttgct acaatatctt cttcccaacc ttctaagaac caccacggtt caaaatctcc
    28561 gtacatttct ataacacgat acatattcat tcattccttt gtaccgtata ttataaccaa
    28621 ttccgccaga caaggaaaga aatatgctca tttctgaatt ttttgataaa aaatccagcc
    28681 atctgatgga tgactgaatt tctatttact tatgtacaaa aatattttta ctacttaggc
    28741 ttctgagata tcgttttcga taatgacctt aatggcatgg tggtctgctg ccttactgaa
    28801 gacttcgtag gctttttcaa tttcactgag tttgaaatag tgagttacca atttttctgg
    28861 ttcaatctta tgactttcaa gtgctttcaa caattgtgga gtcgtatttg tagataccaa
    28921 accagttgtt acattgatgt tgcgaatcca aagtttatct aaatcgaatt caactggttt
    28981 accatgcaca ccacagttgg caaccgttcc gtctacaccg ataatctttt gacagaaatc
    29041 aaatgttgca ggaataccaa cagcttcgat agcgacatcc acaccacgac catctgtcaa
    29101 atcataaatt tctttaatgg ctttttcagg gtctgaagaa ttaaccttat gagtcgcacc
    29161 gaatgatagg gcagtttcca agcggttatc gtctaggtct accataatca atttagctgg
    29221 tgaatagaat tgggctgtta aaagagcagc caatccaact ggacctgaac caataatggc
    29281 tacgctgcaa ccaggttcta ctttcccttt taagacacca atttcatatc cagtaggcag
    29341 aatgtctgac agcataacca aagcttcatc tgacaagtct tctggagtat ggtaaagagt
    29401 attatctgca tgagggacac gtagatattc agcctgcata ccatcaatca agtgaccgaa
    29461 aatccagccc ccttcgtctt cacagtgggc ataaattcct tttttacagt agtagcattt
    29521 accacaggca cagacgcaag aaatcaagac cttgtcacct tttttgaagt tggaaactcc
    29581 ttccccaact tcttcaacaa tcccaatccc ttcgtggcca agaatggtac cactttggca
    29641 agtaggaaca tcccctttga taatatggag gtctgttcca caaatagtgg tttttacaat
    29701 acgcacaata gcgtctgttg gcttgcgaat aactggtttg tctacatcaa caaaagaagc
    29761 aagtcctggt ttaacataag tataagcttt cataaagcac tcctccgttt ttttatttgt
    29821 actatttata gttcaattgt aagccttttt ctttgtttct tcaagctttt tttgtgattt
    29881 ttttaacttt ttatttacca gtaaagtacc ctgcataaaa aagcagaagt tagtctctaa
    29941 cttctgcttt ctctcttatg cttgataacg tttcacgcca tctaggtagg ttgctaccaa
    30001 ttccaaatct ttatctaata cgatgaagtc agcgtcgtag ccttcacgga tttggccaca
    30061 gacgtcatcg atgtgaacgg atttagctgg gttgaagctg gccatcatga ctgcttcatg
    30121 cggattcgca attccccatt cgaccacatt cttcaaacca tctttgagtt tgaggataga
    30181 acctgccaag ttacctgtcg atttgaggcg tgcagttcca tttgcaacga caactgggaa
    30241 ttctcccaac atataatctc cgtcttccaa tcccccagct gtcatacagt ctgtgataag
    30301 agcgatgttt tctgttcctt tttgtttgat aagaatttcg caagcctttg gatctacgtg
    30361 gtgaccatca cagatcaact ctgcataggt atgtggcaat tggtacatgg ctccaaccat
    30421 acccaattca cggtgagtca acccacgcat tccattgtag gcatgcaccc aaacactcgc
    30481 tccagcatcg actgcttttt tggcttcatc aaaagtcgcg tttgaatgtc caagagcaac
    30541 cgtcacacct tcgcccgtaa ctgtacgaac aaagtcttcc accccatcac gttctggtgc
    30601 aagggcaatt ttattaagca agccatttgc cgctttttgc caagaatgaa actcctcaac
    30661 acccgggtct ctcatataag ttggattttg tgccccctta aaagtttctg tgaaatatgg
    30721 accttcataa taaatcccac gaatcttagc acctgttgct tctttataat ggtttccaag
    30781 attttcagtg actgcaagca attgctcata agtggctgtt aaagttgtgg gtaagaaact
    30841 ggtaacaccg gtactaagaa gtccttcact catagtatgc aatgtacctt caatgttgtt
    30901 gtccatcaca tctacacctg catatccatg aatatgagta tccacaagac ctggggcaat
    30961 gctataacct gtatagtcaa tcacctcagc cccttcagga atctgctcta catgttttcc
    31021 aaacttgccg tccacaagtt ccaagtaacc acctcgacga actccgtgtg ggtagaaaaa
    31081 ctgatccgct ttaatatagt taggcataat gttaacctcc ttaaaagatt gattctacaa
    31141 tttgttatat caattacatt ataaaccttt cttttttatt tgtaaatggt atatacctat
    31201 tttttggaga tattttaaaa gagctggatt tctccaactc ttctcttttt taatacaagt
    31261 tattttgatt taaccggctt gtcttgagct gtctgcaaag ctgtggcaat cgtatctgca
    31321 tacaattttg ctcctgcttc gatagtgcta ctctcactcc cgaaatgaac ctgatctgtt
    31381 ccagcccaaa tttctggatg ctctttcgca acttgattcc aatctgctat cgtaatgtaa
    31441 ggtgtcttct ctgccaattc tctcatatag gcagcagcct tctcaacgat ggcataggtc
    31501 tcttttgtct tatctccctc ataaggagtc accaaaatca tatggtgtcc cttaggaaga
    31561 tttttcacga tactgtccca gtcatccttg tagttctcag gattatttac cccagtcgca
    31621 atgaccaccg tcttaggtaa aaatttattc tggctattat ttagcatgat ttcatttgcg
    31681 gtcttggttg ttacgctgac ctgcgcgtta atctgtgctc caggaagagc tgtctgtagt
    31741 gctgtatttg cccttaaagc cactgagtca ccaattaaca tagtgccatc agcaattccc
    31801 aaactgtttg catctgcccg ttctgccatc accttggtct ggccaatatt tgttgcagct
    31861 tgcttcaagc cattgacagt caagtctgtc tcaaacgctc ccacttgtgg tgccaacaag
    31921 gtcaccatgc agacaatgaa ggtcaagatt cctgtacctg ctgcaagaat tgcgtgaata
    31981 taaggcaggg gacgaagggt ttggacaata ggtgtgttct tgcctgcaat ccaaggttcc
    32041 aatacataaa atgacagact ggcaaagcca taagaacaaa tcagagtcag taatacagca
    32101 agaagatttg atgtcaactg tgagaaaatg atatagaaag gccaatggaa aagataaacc
    32161 gcatagctag tatccgctaa aaagctgata atcttcggct cctgtatatg atgtgtcttt
    32221 tcatgtaaga cacgcgccgc cagaatcatg gcaagagctg caagactggc aagcaagaag
    32281 ccgataagat aggcaaaaag ataagtgaat ttgacaaaga aagtcaaaag aactaggaaa
    32341 ccaaagcctc ctccaaaaac taccaaagtc tttcgtaaat cccagatttt atccaactgc
    32401 ttgacgaggg aagtcgtctg acgaacgcct acaatagttg ctaacatact tcccaaaaag
    32461 aatggataga catgagttaa actggagaaa taaacagagg aataagaggt cactagaaaa
    32521 ctaccaataa acatggagaa gaaactgatc aagaaggcag tagcagataa gagaaagatc
    32581 atccccttca actgaccatt tgatttagct tgtttggata agaaccaaac tgccaatccc
    32641 caaagaatat agtagtgaac ctcaactgcc aaactccaat tatgaacaaa caaatgagga
    32701 atgaactgag attcataact cccacctgtt aagagttcat agaagttggt cataaagcct
    32761 aagacgcccg caatctggcc accaattcca gcaacatagt cttggcgaac caagaaagta
    32821 aaaggcatgg tcaccaagac catcaaaacc acaggtggca caatccgata aaagcgtctt
    32881 ctaaaaaatc ctatcaaatc aatctcattg ttcttagaaa attcttcaat gagtagagct
    32941 gtaatcagga aacctgaaaa tgtgaaaaag acatctaccc cgaaaaatcc tccaggaaag
    33001 atcgtctgaa agaagtgata caagagtacc aaaagtaaac ctataatcct aatcaaggaa
    33061 aaccatttaa tgcgcatacg agtttattct tcctttcatt gtacacttta ttctatcaaa
    33121 aaaagaagaa aaatcctaag agaaaactta ggatttttag cttatatcaa ttccatttta
    33181 gaaattacgt cctgacttat tatagccata tttttccaca aaatacttac ggaattccaa
    33241 gagattgtca tccatgatgg cttgtcgcac ttgcttcatc aggttaagca agaagtaaag
    33301 attgtggtag ctagtcaagc ggataccaaa ggtttcatca gccttgagca ggtgacgaag
    33361 gtaagcgcgt gtatagttat tacatgtgta gcaatcacac tcaggatcca gtggcgtaaa
    33421 gtcctcagca aactgggcat ttttcacaac caaacgtcct tgactggtca tacaagtccc
    33481 gttacgagca attcgagtcg gtaagacaca gtcaaacata tccaccccac gaatgacccc
    33541 atcgatcaag ctatctggcg ctcccacacc catcagataa cgaggtttat tttcaggcag
    33601 cagttgagtt gtaaagtcca agaccgcatt catctcttca tgggtttctc ccactgccaa
    33661 accaccgata gagtagcctg agaaatccat gctgacaaga tcgtgagctg attggcggcg
    33721 aaggtcttca aatcctgcac cttgcacaat tccaaacaaa ccttggtcat gtggacgacg
    33781 gtgagccttc aaaccacgct cagcccaacg gctggtacgc tcgatcgatt tcttaacgta
    33841 gtcataaggt tgataaaact gaggacattc atcaaaggac atcatgatgt ctgaactcag
    33901 attattctga atagagatgg ctttttctgg ggataggagc atcttagaac catttagatg
    33961 atttttaaag gttactcctt cttctgtgat attacggcta tctgctaaag aataaacctg
    34021 aaaaccacca ctatctgtca agataggctg gtcccaattc atgaacttgt ggagaccacc
    34081 agcgcgtgca atgagttcat ctccagggcg aagccagaga tgataggtgt ttgataggat
    34141 aattcccgaa cccatctcct tcaattcttc aggtgactga gttttgacag tggcttgtgt
    34201 cccaactggc ataaacatag gtgtcggaaa ggtaccgtgg ggagtgatga tttctcctag
    34261 acgagctcct gtgtgttttt ctttcttaat caaacgatat ttgattggtg aatctgacat
    34321 tttttacctc cgaagctggg aaagacagtc ccagttcata cagtgtgccc aaggacacac
    34381 tgtatgattt tatcaaaaaa agtaggaact gtcacgaact atgtataaga actatggtat
    34441 aataagagaa tgaactatcc aaaaattgat ttaaaaacaa ttcgtcagga gtcaaaacat
    34501 tttcaggctg acaccccccg tctctttctc ctctatatcc ttcccagtat gctggtcatc
    34561 ttatctggtt ttcttaatcc cttatctcgg atccacggga ctgttttaga acagccattt
    34621 ttcagcatac ttggccaaat acttcaaacc tacctctttc ctctactagt ctcctttatc
    34681 gggactattc ttttgaccag ttccgtctat gcaacactga cactcatgaa agattctaaa
    34741 acggagccat ctgtaaaaaa cagcctagct ctctttgatg aggagcgctt ttctcaaacc
    34801 tttttgaccc tacttctcaa acgtttctat ctctttttat ggagtatccc taatttactt
    34861 gggatttacc tcctctttta tagtagcttt ttagccaaaa aattcgtcac cctacatcct
    34921 gagtttccaa atctagatct ctcatcagtt gaaaccgagc gtttcctcat ggtctttgga
    34981 ctctactttc ttgcaagtct catcttgatc attgtaggaa atatcctcta tattccacag
    35041 tattatgcct attcacaggt ggaatttctt ctctgttata gtttagacct aggacaggtc
    35101 ccaccaagac gaatcctaaa aactagccgt tcctttatga agggctacaa gttccagcac
    35161 tttgtcctag atttacagct tcttccttgg tacttcctca attggattac ctttggcatt
    35221 gctagttttt cactcctacc ttatatccaa tgcactaaaa ttatgtttta ccgagcagta
    35281 ttggctcgaa aacgtccaaa agcttgaagg ttttcccctc aagctttttt ctatcaatga
    35341 atttctccgc ctaccaactt gagttcctga tctccatgtt ctatcatagc gccgattgct
    35401 gcttctatcc ctcgccgaat atccactaaa ctcatagttg gagtagtcgg tctgttcacc
    35461 acctgttcca tcatataagg aatatgcata aaacctgcct taacatatgg aaatttcttt
    35521 tctaccaaat agagagcctg atacatcaaa tggctgcaga caaaagtccc tgccgtattg
    35581 gaaacagagg ccggtaatcc ttatttttta tagcttgaac catcgcttta atcggcaaac
    35641 tactaaagta ggccgaagca ccatctgggc gaatgggacg gtcaatcggt tgattatctt
    35701 cgttatcaga aatgcgtgca tcgtcttgat taatggccac tcgttcaggt gtcaaactag
    35761 ttcttccacc agcttgccca atacaaagga caaagtcagg ttgataacga ttcatctctt
    35821 cttccaatac ttgagcagat ttgtgaaaaa ctgtcggcac ctctagccaa cggacctcag
    35881 caccatggat ttcagctggt aaacctttaa tggcctcaaa agggctcaaa acctgtcact
    35941 aatactttca tttctaactc ctcaaaacca attcaatgaa acttttttct taaaaacaag
    36001 tataacatat ttcccttatt ctggattaaa aaggattagc gagattagaa tcgaccttca
    36061 agtgtattta aagagtaaaa tactaatcaa agccaaaata gctgatccac cttgtttcaa
    36121 aataattttt ttatccgctg ttaaagagcc gtaagtcgca gcaccaatca caaataagac
    36181 aaaaatagtc acaatttcta aattctgtga gaaataaatg acatacaaga gaaagactcc
    36241 tagcagagcc ttataaatcc cttgattttt gaacaatgaa cttactgacg gatgagccaa
    36301 ttcttccttt tccatattaa atacacgact agtcgcatct gattgcgtgg caatactttc
    36361 caaataaaaa atgtaaaaat gctccaaagc aacgatcgtt gttaaaataa ttgtaataat
    36421 tgacatctat ttctccttaa atttctatat tttcaatacc caaaaccaat ttatttaata
    36481 aacggctgag ttctgttctc tcaaactctg ttaagatact ttccatctct tccttaacct
    36541 tgatatgttg cagaggggga ttcaccacta actgctcctt ggcatacttc gcagtctcta
    36601 ccaacacttc ccgctgattt tcaggattac gatgacgctc caccaaacct tccgtttcca
    36661 aaatcttgaa atgccgtgtc aaagcagcct gatcaatttt caaacgctcc tgaaccgcca
    36721 tttggttaca aggagaatgc tccagcaaaa acagtaaaat ctgataccgt gtcaaactaa
    36781 tccaagcctt ttttcaaaca attgcgtact cgcttgctca gacaagcgga gttgatacag
    36841 taaatcttga atctctagca tttcttcccc tctcttttta ttgatttatc aatatttgac
    36901 taatcacgta taatgcaaag taaaatagat tgcaagaatg ttacttaact cattacaata
    36961 tggtaacttc ccattttcca ttcaaattga tactcaatga aaatcaaaga gcaaactagg
    37021 aagctagtcg caggttgctc aaagcacagc tttgaggttt catataaaac tgacgtggtt
    37081 tgaagagatt ttcgaagagt atgacaaaga aaaaacgaac gagaatcgct ttccatcacg
    37141 aaagctagat ctcattcgtc aaaatgatgt tatcaagtcg ggtttatcca ttcattgaaa
    37201 cgtgaacgtg atcatagtga ttttctgtca cactaccacg gtctggcatt gggttccaag
    37261 tgttagctgg cccatatttg ctatcgaatg gagcatagaa acgttgtttc cagatgatgt
    37321 aactaatgcc acggctggcc atgttttgaa tagcatattc cgcaatctta tcccctaatt
    37381 ctgaacgttc tgctaccata aagtcgatag ccaaaccttt tccgtgatct ccactgtctc
    37441 ctggacgata accactaaag gatgtaatgc caaacaagtt agcaatttct tctttaaagg
    37501 cagctgtttg tggttgaaga cctgcatttt cagattttgc tactgcaagt ccagcataat
    37561 cgggcgcagc tggtgcctcg taagttgttg aaattacttt cgtttcttct ggttgataag
    37621 tagaaactgc tgctgtagct ccatttgatg atgctacttc ttttgcttct gaacttgttg
    37681 tagttgcttc cactgctgat gcagcttgag ggcttgcttg tgtttcctgc ttcttagctg
    37741 gagtcgtttc ctgaggagtt gcttcttcaa ctgggcgagt tgtttcggcc gtttgctcct
    37801 ctgggacaga agtgcccgta gatggtgcca cttcttcaga agcaatcact gtctttgtaa
    37861 cttctgaact tgatgctact tcttttggag cttctgcaat cggttgagaa aggtctgcaa
    37921 cctgaacagt ttgaacatca acggtcactt gattagtggt caaatctgct gtcgcagttg
    37981 tcacttcttc actagagtct gcttgaggtg tttggatttc aacttctgtt acttcttctg
    38041 cttcattgac agtcgttgtc aaaacagttt ctgggaaaat caagtccata ttagtgattt
    38101 tgttcagatt cgcaagcact gtgacatcta cacccaaggc ttctgcaatg gtgctcaaag
    38161 tatcaccata ctgtacggta taacttgttt tgttgtccgt tttagtcaaa tcgttttgga
    38221 tttgctcaac actacgtgca gtccaaagaa cttcttctgc ttgagttgcc aatactgggg
    38281 caaatgacaa ggctactgtt gacgctaata acattctttt cttcattctt tcaaattcct
    38341 ttcaaatgag tacctgtcta tcataacaca aaaaatgtag aaaaaaagcc ctctcgggcc
    38401 tatttaacag aactgtctat tccttgtaac aaactaggtt acttgaaata gtttgttagg
    38461 tctctgtcac aaaactgaca caatcttttt atcacttagt ctcaaaaata cgggaaatat
    38521 ctgctaacgc ttgaatcctg tgattccctt cataagtaga ctctaaaaag ttgatacttt
    38581 gaatcccact attctgggca aattccacat ccagagtccg atcccctata taataagtat
    38641 tatcagaatt caactgatac ttatctagca gataggtagc cgcttctgga cttggcttcc
    38701 gcacaaagcc actctgactg gttaaaatct ctgtaaaata ggattccacc cccaagtcct
    38761 tgagaatggt aaaagcgttg ttccccttat gagtatatat aaactgctga attcctgatt
    38821 cgtctgccca agctagcacc tcacgcgcac ctggcatcaa aactacctga gcattcttct
    38881 cagccagact ctgggcacgc acctgattta gcacctcaac atccagattt ctatcttctg
    38941 ccacccgcac aagcaaatct tgcaccgaat acttgaagat aaactctctc accttctcct
    39001 tatcataagg aatagaaaac tgagcaaaag tctcctcaat ccctgataaa atcgcttcgt
    39061 aagagtccaa taaagtcccg tctaaatccc aaataaaagc tgttttttgc atttcctatc
    39121 ctttcaagct catatagcgc tggtaacgat agcgtagaaa taaccagcga aaaccattat
    39181 ccaagagaga ccctgcccaa ataccaggca agccccaacc aagcacaatc cccatcagat
    39241 atcctgtccc aatgcggata caccacattc ctatacttgt cgcataaaaa gggaggcgtg
    39301 catttcctaa tccctgccag actgccgtat agatgactgt tcctgtcgtc ataggggtcc
    39361 caagtagtga aaacagtgtc actagaacac tagcctccac cgctagagaa tcagtcgtat
    39421 agagatgagt taatggtaca cccaagacat atatactaaa ggacaggggc aacatgagga
    39481 acagagaaag ccaaaaggtt tgtttactca aactagcaac tcttttccaa tcatcctctc
    39541 caactgctcg ggccaacagc atgaccgttg ccgtagcgac gccaaaggca ggcatatagt
    39601 taaactgggt caagacttct ccgattgcat tcccagcaac tgcctccgtc ccaaaagaaa
    39661 cgaccaaggc aatgatcact acatctccag ccctcatcat aagtcgctct ccagctgctg
    39721 gtaaagccaa ggtcaacagt tccttatcta aaccaaaagt tggcttccca taaggcagtt
    39781 ttaattgtga ccacaaaatc acaagaccaa ccaaacgaga cacaattgtc ccccaagcaa
    39841 caccagctat ccccatatcc agaacaaaaa tagctagact tgaaaaaaga atattcaagg
    39901 cattggataa aaaactaaca tagagaggca gacgtggatt atgcgttgca cgaatcaagg
    39961 ctcctagact agtcattaaa cctaagagaa caatcgatcc gcctaccaaa gatagataca
    40021 gtccaccact ctcagctaca tccctctccg tccccaaaag tcctatcatc tctttcccag
    40081 cgaagatgga caaaaatcct aaaaggaaac ttaatagtaa ggtaatcttc aacgcctcag
    40141 tcacatgata ggccaacttc gactggtctt tctgccctat gctttttgaa ataacactgg
    40201 aaatagcagc tcccagagcg atgaaaatcg cctgataaat ggtgataata ttaccagcta
    40261 ctgaaacccc tgaaatagct atcaatccta aatgagcaac caaataactg tccaccattc
    40321 ccattagcat ctgcaaaaag ttttcaccca tagctggcaa tgcaatatta agaatgtctt
    40381 tatttttctt aaacaatctc tcctcctgat gaaaagaaac tcagttggtt tcccaaccga
    40441 gtttgctccc tctgtcttaa agtcctaaat aagcctcaac cgctgcttgc atgtcagcag
    40501 ctgccactgt tgtcttgtga cgaattggag ctatttcaag cccatcaact gctggtggca
    40561 ctgcaacgcc tgagatttca tgtaattgag ccaaggcttc aaagtctgtt aaacctgctt
    40621 ttccagttac agcttctact gcaactactg ggaacttgta tggactagct gttgaagcaa
    40681 tcactgtctt agttacatct ccagtggccg attggtattt tttataaact gctgaagcaa
    40741 cagctgtatg agggtcctcg atataagaat ctaactcaca aacacgcttg atctctgctg
    40801 ccgtttcttc ctcagtcgca tattcagctg caaagaggtc caaaatctct gcatcaaagt
    40861 ctgtcaactt atattgtcct tgcgtgttca aggcattcat aagttcagtt gtcttttcag
    40921 cattatttcc caaaagatgg aaaatcaagc gctccaagtt tgaagatacc aagatatcca
    40981 tagatgggct ggttgttacc ttaaactcac gttttttgtc atagacacgt gttttaaaga
    41041 agtctgtcaa aacattgttg tcatttgaag cacagattaa tttaccaact gggagaccga
    41101 tttgtttggc ataaaaggca gccaagatat ttccaaagtt tcctgttggt actgtgaagt
    41161 taatcttatc accagccacg atctcaccag tcttgaccaa ctgagcatag gcataaacat
    41221 aataaacaat ctgtggcacc aaacgaccga tattcataga gttagcagat gaaaattgca
    41281 acttgttggc cgctaatctt tcacgaagag ccacgtcgtt aaacatgtgc ttcacgttgg
    41341 tttgtgcatc gtcaaagtta ccatctatag cgataacatg agtattgtca ccattctgag
    41401 tggtcatttg caactcttgt accttgctga caccaccctt tggataaaag acgataatct
    41461 cagtaccagg cacatccgca aaccccgcca tagcagcttt ccccgtgtca ccagatgtcg
    41521 ctgtcaagat aacaatcttg ttctccaaac catgtttctt agcagcagtc gtcataaagt
    41581 atggcaaaat agacaaggcc atatccttaa aggcaatcgt tgaaccatgg aaaagttcca
    41641 aattgtattg cccatctaat ttcactaatg gtgcaatagc tggagtatca aatttgctat
    41701 cgtaggcatt gttgatacag tagtccaact cctcagctgt aaagtcatct aaaaatgctg
    41761 acaaaactag cttagcaact tcctggtaag aagcatcttt caatttgtca aagttcaaat
    41821 ctacctttgg ataagtaacc ggtgtaaaca aaccgccgtc cgtcgccaaa ccttgcaaaa
    41881 ttgcttggct ggcagttact gtattgttgg catcacgcgt tgattgataa actaatgtca
    41941 ttactctcta tcctttatct atagtctcgt ccattatatc atgatttttc agaaaattct
    42001 ccctttataa gagaaattat aggctcaatt cttctctcaa aggctgtaaa taggtcattt
    42061 cttccttatt tctcaccact agtccctcct cagctagtag gagtaagtct ttggaaaatt
    42121 caataatcgt agtttcttcc tcatctgtaa gatttttctt agaaaattgt ctccttaaag
    42181 acttgtaatc ataaccaaat actttaaaga aaggtgctgt ttctaagtaa gcttctaact
    42241 tgtctaaatt aaccaataat cccaagtgaa aagctgcaga agcaaaagtc ctatcaagtg
    42301 gctgtgtaca cacactacga aactcaactg ttcctcgagt cgttaagtct tggtactggt
    42361 aactacgatg agtttcaaaa tccttctctt ggggggaaat aataacctca tccccattca
    42421 gagcaaatgc ttggatttca ggcgtagcca aatagtcccc agcctgaata ggataaaaat
    42481 aataggtctg cccatcacgt tccgcagtaa aaatcgcaga atgatttaga tagtcaaaaa
    42541 aatcagcttc atcattaagg agtctagcat tgaccccaac attctctgga tagataccat
    42601 gcatagattc ttcccagaaa atatcccttg aaattttcgt atcccaatcc gcacccgaaa
    42661 actcagagtt tgcaaataaa taagccttag ccgcttcaat ttgagtaaaa gcattaatca
    42721 cccgtaagta gttggttttt gaaatatcca gctgaacctg gctcccacag ataaaagtac
    42781 catattcagg gaaatgatgt aaatctgatt taataatatt tctactcaaa ttcaaataat
    42841 ccatcaacat ctgatagcgt ggataagcca ctggacaatt ctcattttta tcccagttgg
    42901 gatggatacc acagccaaca atagcatgat ttgattcagc taacttcttc tggattgctt
    42961 ccatataaat actaaaacgt tcttcaactt cttgtatcgt ttcagcctta ccaaatgcaa
    43021 actcaatcgt tgtataggaa acttcaaata aaatagcatc ctgacttatc ggatctacta
    43081 actggatcaa attgccaaaa tcatctacct ttgctacggt gagatccaaa gtagaaacta
    43141 aataatgaaa tagatccttt ataacttcaa catctgtagc atccccttct aaacttgcaa
    43201 caggatactc caactcaatt ccgacaaaca attcaggatt ttctttaata tttttcaaat
    43261 accgatgttt taataaatcg atagaacaag acatactacc ctacactttc ttaatctaaa
    43321 taaaatttga ataactataa ttataccaaa aataatataa aaaaggaacg aagaataaca
    43381 acgttcctaa cactatacta taccggcggc cggggtcgaa ccggcacgtc cttgcggaca
    43441 ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaaatag taactggggt
    43501 agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata
    43561 ccccaatata aaataggcga gtgatgggga tcgaacccac gcatgccaga gccacaatct
    43621 ggtgtgttaa ccacttcacc acacccgcca taattctatt aacacgggca gtaggaattg
    43681 aacccacact gaaggttttg gagaccttag ttctaccttt aaactatgcc cgtaaaatgg
    43741 aaggggaggg attcgaaccc ccgaacccga aggagcggat ttacagtccg ccgcgtttag
    43801 cctcttcgct acccttccaa aatata
//
LOCUS       DAVKGU010000006        43817 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKGU010000006 DAVKGU010000000
VERSION     DAVKGU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43817
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.006"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            85..237
                     /locus_tag="VW077_000307"
     CDS             85..237
                     /locus_tag="VW077_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454393.1"
                     /translation="MSYTVTLFFDNMVDETHFFKKESDAAKCKAQLESKYRGNRMYKV
                     KMEEME"
     gene            237..575
                     /locus_tag="VW077_000308"
     CDS             237..575
                     /locus_tag="VW077_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454394.1"
                     /translation="MSYDLEILAKIESGDYICIAEPRYSSPTYNLGKMFRVAMDWDFD
                     QGTIYNVADIFENIKRGITELERQPEKYVQYEPANKWGTINDALYVLRSLRDCILEQD
                     IDTKYLYVRW"
     gene            726..1040
                     /locus_tag="VW077_000309"
     CDS             726..1040
                     /locus_tag="VW077_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454395.1"
                     /translation="MEELKRKVNTVYNWTVEDGKPQPPKQDLPQVVKDRADYFWEMAE
                     DGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWVGYSPSMAQVVIAVYLIYR
                     GN"
     gene            1042..1569
                     /locus_tag="VW077_000310"
     CDS             1042..1569
                     /locus_tag="VW077_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9454396.1"
                     /translation="MNKQELIKKLEERRTITGNFQGYVVWWKDVKEIFEQLGEPQPVK
                     VPQFVADWIRKCKMFKHFAVSLSFALQPIVWEENGLSHECIEWLMDANNQELFALAWL
                     NGYEVEKEKRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEDANF
                     GWVFDCEGIDIEEVE"
     gene            1631..1948
                     /locus_tag="VW077_000311"
     CDS             1631..1948
                     /locus_tag="VW077_000311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9454397.1"
                     /translation="MRYFKILCIVLFASLLVACHQISSGTVVDKYIDEPHTTFIPVIN
                     GKSSVLVPTRTKRKYILVVSGYAGNKQVEERFEVTANEYKHYEIGNTFIQDAVLENKE
                     EDK"
     gene            2066..2488
                     /locus_tag="VW077_000312"
     CDS             2066..2488
                     /locus_tag="VW077_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU9454398.1"
                     /translation="MNKAKELLKELQNLDMDIQSRIDEINELEAGLLSSPKWIDVKVQ
                     GGQARKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRLINKLKNPKSRSILRVTYITK
                     MYVDDICDKMEISRTTFYTWRNMAISELNEVLERMELN"
     gene            2574..2656
                     /locus_tag="VW077_000313"
     tRNA            2574..2656
                     /locus_tag="VW077_000313"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2605..2607,aa:Tyr,seq:gta)
     gene            3758..4270
                     /locus_tag="VW077_000314"
     CDS             3758..4270
                     /locus_tag="VW077_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEU9454399.1"
                     /translation="MAFFDCLKGGDGKLNERQRRFADEYIISGNAYQSALRAGYSEKY
                     AKARSSELLDNVGISDYIKNRMEELQDEKILTQKQILVMLSEIASGQAKETIVVTTKV
                     AELMTDPVTGKSVKVYNEIPQLVEYPTKNSDRNKALELLGKRHKMWTDKVEADVSGTV
                     VFANESDISD"
     gene            4248..5546
                     /locus_tag="VW077_000315"
     CDS             4248..5546
                     /locus_tag="VW077_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEU9454400.1"
                     /translation="MSQTYQIKQNDIVVDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETSRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFDKDELLRDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIVADSAELRL
                     IEELRSEHGITRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            5558..7027
                     /locus_tag="VW077_000316"
     CDS             5558..7027
                     /locus_tag="VW077_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9454401.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHNTESSRLKNLKSYYM
                     GDHAILNHKRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADSDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKYQAEVFTENLHYHMVLRSTDSGTTQNEQVTPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            6936..8573
                     /locus_tag="VW077_000317"
     CDS             6936..8573
                     /locus_tag="VW077_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEU9454402.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEV
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPESEIKHLQSFSWVGDGSTYSTDIWKNTGKLTSSIKDELLMSLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFVAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVAYDEDADYSKLKRRARNPVTGKTELVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKIIADSLVENISLSKAVSKINDALRYTTIFDSDTFTEEYLKMK
                     QKLVAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNGSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            8575..8784
                     /locus_tag="VW077_000318"
     CDS             8575..8784
                     /locus_tag="VW077_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454403.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSIDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            8781..9194
                     /locus_tag="VW077_000319"
     CDS             8781..9194
                     /locus_tag="VW077_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9454404.1"
                     /translation="MIDIALAIAKKAHAGQVDKADVDYIQHPLYVASQVKTEQEKAVA
                     LLHDVIEDSDITAADLLASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIYYLST"
     gene            9236..9409
                     /locus_tag="VW077_000320"
     CDS             9236..9409
                     /locus_tag="VW077_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454405.1"
                     /translation="MDKAKIGITNVEFSGTGGIESATVKLELNIYGADTFSAIELLPK
                     ILTDIHSLSYEVD"
     gene            9676..10200
                     /locus_tag="VW077_000321"
     CDS             9676..10200
                     /locus_tag="VW077_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEU9454406.1"
                     /translation="MSEEINATVSIESTETVDTQGNVDSVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTNAETVSESITSIQKAWEESVQKGVSERMKGSAPIKNAQT
                     VQQEVTEKWRKDFL"
     gene            10223..11248
                     /locus_tag="VW077_000322"
     CDS             10223..11248
                     /locus_tag="VW077_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEU9454407.1"
                     /translation="MAFEELNTAESRKKHLGIIEDVLAVNSYSTPLVTSSDAVTLQGR
                     SFTVATGNTTELKDYKRNKDNEFDHVEVEEKVYTLDEEKYWGRFVDQLDERDSNGQVN
                     INYVIARQAAEVVAPYLDELRFSAALGNVSDNVTMGKEAGANNAYNAILDVSEKLDEL
                     GITKERLLFVTPKFYKAIKSEIVRLPHGDADKKVLGKGYVGELDDYTVYKVPSKFLKG
                     VNALATAPGVVTSPVQVDNTKYNDNIPGRFGELVEQLLYTGAFVLEHFKKYIITIADS
                     KPAAKKSAQGKTVNRAKAWKTGTAYKEGDTVTHEDKVYVAIKDITSSTNAPGSDSANW
                     KEKTGKK"
     gene            11258..11446
                     /locus_tag="VW077_000323"
     CDS             11258..11446
                     /locus_tag="VW077_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454408.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVA
                     ISDVLEKILPEPEFLEED"
     gene            11448..11759
                     /locus_tag="VW077_000324"
     CDS             11448..11759
                     /locus_tag="VW077_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU9454409.1"
                     /translation="MSIELLKKLTGEEDTQLLMLFQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGERSRTEGGIAVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            11740..12078
                     /locus_tag="VW077_000325"
     CDS             11740..12078
                     /locus_tag="VW077_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454410.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            12053..12568
                     /locus_tag="VW077_000326"
     CDS             12053..12568
                     /locus_tag="VW077_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU9454411.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELVISIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQANHSGISPEV
                     SVTYKSNPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            12565..12933
                     /locus_tag="VW077_000327"
     CDS             12565..12933
                     /locus_tag="VW077_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454412.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            12936..13349
                     /locus_tag="VW077_000328"
     CDS             12936..13349
                     /locus_tag="VW077_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9454413.1"
                     /translation="MLANGIKLAFSETKGNYQNLVGLKEVPEFGIEPEKVENTTLADT
                     VKKYEFGIGDAGELEYKFAYNNSSATAPYRVLRKAADGKKKLYFEQTYPDGTKVTFEG
                     QVSVKLGGGGVNAVIEFTLKIALQSELTFVDGIGG"
     gene            13354..13734
                     /locus_tag="VW077_000329"
     CDS             13354..13734
                     /locus_tag="VW077_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454414.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVVE"
     gene            13773..14123
                     /locus_tag="VW077_000330"
     CDS             13773..14123
                     /locus_tag="VW077_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454415.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYI
                     LSRMITNHVSLLLSNDAKIVELWEYAPELFVEEQQAVEQERQRQALLLHKERMRDFAE
                     RHNRKRKEEVNGNS"
     gene            14110..17703
                     /locus_tag="VW077_000331"
     CDS             14110..17703
                     /locus_tag="VW077_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454416.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIKSTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGNTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSIATIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNVGNLSEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVVPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLAYAIKLVADGFTVFSDWCKENKEPIVALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKVSLIVGGIKNLGLAIKALTFDKLVSFAETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICQAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIGNIFGNLGSWFGGRWNDSKNALAEANTWLGDKFKS
                     GRDKVNSAFEKVGSWFGDRWNDIKDGVKEADTWFGEKFESAKKKTQNPFQKIGSWFGD
                     RWKDMQDALKEIPNWFKNLFNDAMDNAKNIVKSGIDKLKSFFNFDWSLPKIKLPHFNI
                     SGSFSLMPPRIPSFSVDWYARGGVFNSPSIIGVGEAGQEAVMPLERNTGWISILAQKL
                     AERMPANNVPTGYSLPAGDIVIQIAGHEFGRVAIQEINKEHERAGQTLLKI"
     gene            17712..18062
                     /locus_tag="VW077_000332"
     CDS             17712..18062
                     /locus_tag="VW077_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454417.1"
                     /translation="MAQLTINGVAVKPPKSFQVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSEFFEVSYPDPMDGQVTKTFYVGARTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            18067..19935
                     /locus_tag="VW077_000333"
                     /pseudo
     CDS             18067..19935
                     /locus_tag="VW077_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339666.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
     gene            20431..24510
                     /locus_tag="VW077_000334"
                     /pseudo
     CDS             20431..24510
                     /locus_tag="VW077_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            24604..24807
                     /locus_tag="VW077_000335"
     CDS             24604..24807
                     /locus_tag="VW077_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454418.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            24810..25160
                     /locus_tag="VW077_000336"
     CDS             24810..25160
                     /locus_tag="VW077_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454419.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            25170..25469
                     /locus_tag="VW077_000337"
     CDS             25170..25469
                     /locus_tag="VW077_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454420.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHAVGDIQKEMVRMNDRMEEIEKGE"
     gene            25473..25808
                     /locus_tag="VW077_000338"
     CDS             25473..25808
                     /locus_tag="VW077_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU9454421.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKIRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGQE"
     gene            25808..26764
                     /locus_tag="VW077_000339"
     CDS             25808..26764
                     /locus_tag="VW077_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9454422.1"
                     /translation="MDIDTSRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGFYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYWFDNSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGSMADKPEFTVEPDGLITVK"
     gene            complement(27017..27985)
                     /locus_tag="VW077_000340"
     CDS             complement(27017..27985)
                     /locus_tag="VW077_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9454423.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            28121..28978
                     /locus_tag="VW077_000341"
     CDS             28121..28978
                     /locus_tag="VW077_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9454424.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            28965..29744
                     /locus_tag="VW077_000342"
     CDS             28965..29744
                     /locus_tag="VW077_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9454425.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            29768..31120
                     /locus_tag="VW077_000343"
     CDS             29768..31120
                     /locus_tag="VW077_000343"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9454426.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSVIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            31194..31568
                     /locus_tag="VW077_000344"
     CDS             31194..31568
                     /locus_tag="VW077_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9454427.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            31570..32418
                     /locus_tag="VW077_000345"
     CDS             31570..32418
                     /locus_tag="VW077_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9454428.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            32684..33451
                     /locus_tag="VW077_000346"
     CDS             32684..33451
                     /locus_tag="VW077_000346"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9454429.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWIDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            33463..34647
                     /locus_tag="VW077_000347"
     CDS             33463..34647
                     /locus_tag="VW077_000347"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9454430.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNLQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            34644..36515
                     /locus_tag="VW077_000348"
     CDS             34644..36515
                     /locus_tag="VW077_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9454431.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLESTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(36658..37842)
                     /gene="mntE"
                     /locus_tag="VW077_000349"
     CDS             complement(36658..37842)
                     /gene="mntE"
                     /locus_tag="VW077_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9454432.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            38146..40842
                     /locus_tag="VW077_000350"
     CDS             38146..40842
                     /locus_tag="VW077_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9454433.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEDHVLTGAELNELSDEDFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            40855..41646
                     /gene="yghU"
                     /locus_tag="VW077_000351"
     CDS             40855..41646
                     /gene="yghU"
                     /locus_tag="VW077_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9454434.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTVGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            41762..42172
                     /locus_tag="VW077_000352"
     CDS             41762..42172
                     /locus_tag="VW077_000352"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9454435.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANHDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            42184..43779
                     /locus_tag="VW077_000353"
     CDS             42184..43779
                     /locus_tag="VW077_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454436.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        13637 a         7958 c        10317 g        11905 t
ORIGIN      
        1 atagaatagg gattcttgaa aaaaactttt tactttttag aatttatcgg tagcacggta
       61 gcagtttaga aaaaagaggt aaaaatgtca tacacagtaa cattattttt tgacaacatg
      121 gtagacgaaa ctcacttttt taagaaagag agtgatgctg ccaaatgcaa ggctcagtta
      181 gaaagcaagt atcgagggaa tcgaatgtat aaagtaaaga tggaggagat ggagtaatga
      241 gttatgattt ggaaatctta gcgaaaatag agagtggaga ttatatttgt attgctgaac
      301 ctagatatag ttctccgacc tacaatcttg gaaaaatgtt tagggtggct atggattggg
      361 atttcgacca aggcacaatt tacaatgttg ctgatatttt tgaaaatatt aaacgtggca
      421 taactgaatt ggaaaggcaa cctgaaaaat atgtacaata tgaacctgca aataaatggg
      481 gaacgatcaa tgatgcgtta tatgttttga gatcgttaag ggactgtatt ttagaacaag
      541 atattgatac gaaatattta tatgtgaggt ggtaaattga aacgaccaaa cagatacccg
      601 tacactaaaa atcaatgggt tgaagaaacc gttgatcact atacttataa aaacgatatt
      661 tgctatacaa gtcacatttt agaaaataga cttactggag aaattaagga caaggagttg
      721 aagtgatgga agagttaaag cgaaaagtta atacagtata caactggacg gtagaagacg
      781 ggaagccgca acctcccaag caagatttac cacaagtggt gaaagaccgg gcggactatt
      841 tttgggaaat ggcagaagat ggtatgacgt ttatgggagc gatggaatgc atcttcgctg
      901 atgaaaagcc tacagactat gatttgggag ctactaagga ttggttgcca aaatctaagg
      961 agtttgatga ttgggttggt tattcgccaa gcatggctca ggtagttatt gcagtttatt
     1021 tgatttatag aggaaactaa gatgaataag caggaattga ttaaaaaatt agaggaacga
     1081 agaacaataa ctgggaattt tcaaggttat gtagtttggt ggaaggatgt aaaagaaatc
     1141 tttgaacaac taggcgaacc gcaaccagtc aaagtaccgc agtttgtggc ggattggatt
     1201 agaaaatgta aaatgtttaa gcattttgct gtaagtctat cttttgcatt acaacccatt
     1261 gtatgggaag aaaatgggtt atctcacgag tgcattgaat ggttgatgga tgcaaacaat
     1321 caagaactct tcgcactagc ttggcttaac ggctacgagg tcgaaaaaga gaagcggtat
     1381 tttgttaaga ttaaagggaa tattaaagaa aatatgttgg tttatggaga acttttgaaa
     1441 aggtatttct ttacaaaaag ctttagttta gacgatgtta tatattccca cacccgtaaa
     1501 gaactagaag acgcaaactt cggctgggtg tttgattgtg aaggaattga tatagaggag
     1561 gtggagtgat ggaagatgag gaaatcatag gcaacatcta cgcaaaccca gagcttttgg
     1621 aggtcaacga gtgagatatt ttaaaatcct atgtattgtt ttattcgcat ccttactcgt
     1681 agcatgtcac cagatttcga gtgggacagt ggtagataag tacattgatg aacctcacac
     1741 aacgttcata cctgttataa atggtaaaag ttcggtactt gtgccaacca gaaccaaaag
     1801 aaaatacatt ctggtcgttt caggatatgc aggtaataag caagttgaag aaaggtttga
     1861 agtgacagca aatgaataca agcactatga aattggcaat acttttatac aggatgccgt
     1921 tttagaaaat aaggaggagg ataaataatg agacctaaaa aatacccaca agatactcaa
     1981 tagctactaa aacaaaaaaa gccaagacac tctctgtctc agcaataatc tcaataatat
     2041 tattatatca caaaggagac agagagtgaa caaggctaaa gaactattga aagagttgca
     2101 gaatctggac atggacattc aaagccgtat agatgaaatt aacgagcttg aggcaggttt
     2161 gctttcaagc cccaagtgga ttgacgtcaa agtccaaggt ggtcaagcta gaaaagttga
     2221 tgatgtctat actcagcttg tcgtgatgaa agaggctata gaacaggata ctaaagaggt
     2281 tatcaacaga aagcttgagc ttggtaggtt gattaacaag ctgaaaaatc caaagagcag
     2341 gtctattctc agggtgactt acattactaa gatgtatgtt gatgatattt gtgacaaaat
     2401 ggaaatcagc agaacaactt tctacacttg gcggaatatg gctatctctg aactgaatga
     2461 ggttttggag agaatggaac taaattgaac tttacaaaac cgtacgggaa aaaatgatac
     2521 ttgttagcac agttttgtaa ttctgataaa atggtagtat caagaattga aaagagaggt
     2581 ctcagaattg gtagatggtt acctgtaatg tcagggggct gtaatggcct tggaggttca
     2641 agtcctcccc tctcctttga gtgtttgtgt cccagaatga gttaaatctt ctgggtgggg
     2701 attcacatat cactcattaa cttctatcac tcattaactt aaaaatggtt gcagtagcga
     2761 ctgaaccttg catgattgcg tagctaatta tattccggat aagttataag ctagagggtt
     2821 tgattccctc agaggttgta aagactacaa aaaataaaaa tgaagtcaaa atttaatacg
     2881 cacgcaaggt agtagtcgcc ttgcactttt agggcttagc ctagataatc tgtggtaact
     2941 aaggaaaagg atgtttttaa atctatcaaa catcctgcca gcaatggtca atctaagcaa
     3001 tgtaatctta actatttcag ttttggaata ggtaggcgaa gttaaagcag gaagattcca
     3061 acggcaaggt gctgaggaaa tgcaaatgtg gcagtttggc tgtgagacga gtctataaga
     3121 ggaatgaggt attcggtttg aggtgcaaca agagcttaat accatatctt acaaaaattg
     3181 ggcgcctccc aaaagtatgt aaggtgagtc gattgtccgc aaaacaatcg ataacaagca
     3241 ggcgctgtgc attttgttct tcaaaagaga atgaaacaca tggcgatgcg tgtctgtgat
     3301 agatgaaaga tgatttttat attttaaggc tattcaagat agaaaaaact caaaaaagca
     3361 aaagtcatcg cccgtcgtaa acgaaagtgc acttcggcaa ttagattgcc tgctcaagtc
     3421 tcgcaaggat gagagtaaag tcaaagagta aagcagctta gacttttagc ggagtcttcg
     3481 ttaattgaaa aatggcctag tagtttgcga tgtgaggagt gattggtcta accaatcgtg
     3541 catgagtgat acaagtagga atatttgtgg acaagataat aaactataag ttatcaaaag
     3601 tcactcgttt aaagcagtag tctcatgcta gttaatggat acatggtaga cggattaagt
     3661 cctgtttagg gaattgaaac gtaggcaggt tcgaatcctg tcgttccaat tgcgatttta
     3721 attcgcagag agaggtcttg aaaaggtcgc acatcgtgtg gctttttttg attgtttgaa
     3781 aggtggtgat ggaaaattga atgaaagaca aaggcgtttt gcagatgagt acatcatctc
     3841 aggtaatgct tatcaatcag ctttaagagc aggatatagt gagaaatatg ccaaagcaag
     3901 atcttctgaa ttgttggata atgtcggaat ttctgattac atcaaaaatc gaatggagga
     3961 gttgcaagat gaaaaaatct taactcaaaa acaaatactt gtgatgctat cagaaattgc
     4021 gtcgggacaa gcgaaagaaa caatagtagt cacaacaaaa gtagctgagt tgatgactga
     4081 tcccgtgact ggtaagtctg taaaagtcta caatgaaatc cctcaacttg tcgaataccc
     4141 aacaaagaac agcgatagga ataaagctct tgaattgtta ggtaaacgac ataagatgtg
     4201 gacagacaaa gtagaggcag acgtttctgg aacggtggtg tttgcaaatg agtcagacat
     4261 atcagattaa acagaacgat attgttgttg acctacctaa gacagtagga gctggatacg
     4321 gacagttctg gcgctcaaga agtctttatc gtgtagtcaa agggtcccgt ggttcgaaga
     4381 agtccaagac aaccgctttg aattatgtta tccgtctttt gaagtatccc tgggccaact
     4441 tgcttgttat tcgtagatac tcgaatacca acaagcaatc aacttatacg gattttaaat
     4501 gggcgtgtaa tgtgttgggt gtgactcatt tgtttaaatt taacgagtct ttgcctgaaa
     4561 taaccataaa agcgactggt caaaaaatcc tattccgtgg tttggatgat gaactcaaaa
     4621 tcacatctat cacggtcgat gtcggcagtc tttgttgggc atggtttgag gaagcatatc
     4681 aaattgagac tgaagacaag ttcagtacag tagttgagtc aatccgtggt agcttagatg
     4741 tacctgattt ctttaaacaa atcacagtca catttaaccc gtggaatgag aggcactggc
     4801 tcaaacgtgt gttctttgat gaagagacta gccgagctga tacattcgct actacaacca
     4861 cttacaaatg caatgagtgg cttgatgaag tcgatattaa gcgctatgag gacttgtatc
     4921 acacaaatcc aaggcgtgcg agaatcgttt gtgatggtga atggggagtt gctgaaggtt
     4981 taatctatga gaacgtgacc gtcaaggatt tcgataagga tgaattgcta cgagattcag
     5041 ctaataagtt atgtatcggt cttgactttg gttttactca cgatccaacc gctttgtgtt
     5101 gttcgttgat aaatgacacg acgaaagaga tttatgtctt tgatgaggcg tataaagttg
     5161 ggttgataac aaaagaagtt gcgaagatga taaaagacaa aggttatcat cgttcacaaa
     5221 tcgttgccga tagtgctgaa ttgcgactga ttgaggaact aaggtcagag catggtataa
     5281 ctcgaattaa agagagtcgt aaaggtaagg atagtattat ggcaggcgta tccaaattgc
     5341 aaggatacgc tatttatgtg catccagatt gtaaaaacat catggatgaa ttttatagtt
     5401 actgctacca gcgagataaa gaaggcaact ggttgaataa accagaggat aaaaacaacc
     5461 acttgatgga cgctttgcgt tacagccttc aatgtatcga aggtggaaaa gcaaccgtcc
     5521 gcagacgttc tgattatggt ctatagagag gaaagacatg taccaatatt taacctatcc
     5581 acgggatgga tatgatgagg gttctttgaa gaaagacctg atttacaaat tgataacgat
     5641 acataacact gaaagctcac gcttgaagaa tttaaaaagc tactacatgg gtgatcatgc
     5701 tattttaaat cacaagagac gcaacgtgaa cgcaccgaat tacaagacgg tagctaatca
     5761 tgccaaggat atcgcagaca cggctacagg ctattttatg ggcaatccta ttaagtataa
     5821 caatactgct gacagtgata tcgatgaact acttacagcc tttgatggtg ctgagattga
     5881 ccaagtagat gctcagaatg ctttgaacat ggctatctat ggtcgtgctt acgagtacat
     5941 ctatgctaaa gagggtatga ctgagttgga ttcaactagt attgatccag aaaatacctt
     6001 catggtctac gatgatagta ttgagcggaa gcctttgttt gcggtctatt actatgaagt
     6061 aaaagacgat acgaaagaca ctaccaagta ccaggctgag gtctttaccg aaaatctgca
     6121 ctatcacatg gtgctgagaa gtacagattc aggaacaact cagaatgagc aggtaacccc
     6181 tcacaacctt gggcaaatcc caattatcga atatcgcaac aatcactttg cgatcggtga
     6241 ctatgagcaa caaattagct tgatagacgc ttataattcc ttgatgggga atcgtgtcaa
     6301 tgataaggaa caggctgtag agtctatcct tgtcttgtat ggcacgcagt tagcagacac
     6361 tccagaagac gctaaggtag cgatgaagat tctttctgaa gaaggtcttt tggaattacc
     6421 gggcgatagt gcaagagctg agttcttgaa gaacacgttg gacgaaagtg ctactgaaat
     6481 cttgcgtaca gctcttaaag aggacatcta cacatttagc catgtgccta atttgactga
     6541 tgagaatttc gcagggaata catcaggcgt agccatggaa tttaagctga tgggccttga
     6601 gatgattact aagaccaagg aagcgaacta taagcgagga ttgcgtcagc gtattgcgat
     6661 ttttgctcat tacttgggta tgaaacagat tgcactagag tctcattcaa tcgttccaca
     6721 attcagtcgt ggtttgccta agaacttgtt agaaatctct cagatcgtga acaaccttga
     6781 aggtaaagtg acgaatagac agcttatttc tctcttgccg tttgtggaag accctgacgc
     6841 tgaactggaa gccttggaag aagaaaaaaa gaagaacatg gaagacatgc cgatgttcaa
     6901 caaagacaac acgaaacccg aagacgaggt agaggatgaa gaatcaggag tattgggcga
     6961 agaggaaagc caatctgatt taccagcaga tggacaaggc cgaaaagcag gcagaccagt
     7021 tcgataaggt ctatcaggaa gccaagactt acttggataa ggaagtcaat aagatttttg
     7081 ataagttcca acgtgattat ggtctaagtc aggtagaagc tagacaagtc ttgaagaaca
     7141 tgaaagacaa gaaaaatctg aatgaacttc gtaaagtgct tgaagcaaga ccgaatgatc
     7201 caaatattca aagactactg gctgacttag atagtccggc ttattctttc cgtatgaagc
     7261 gtctagaacg tttgagtgat gatttagacc gtatgcgtga atctatctat cattcagaaa
     7321 agacaggctc agacgccttt tatagcgacc tgatgaagga tagttactac aaggctacct
     7381 ttgacctgca gcagcagaca ggactagcat acggcttttc tgggcttcct gagagcgaga
     7441 ttaaacatct acagtctttc agttgggtag gtgacggaag tacctactct acagacatct
     7501 ggaagaatac ggggaagctt acttctagca taaaagatga actacttatg agcctcatga
     7561 caggccgaga tacacgagaa actgcacaag caattgctga gaggttcaat gtaggtcaga
     7621 atgatgcaag gcgtttggtt cggacggaat cagccttttt tcataaccaa atggaactac
     7681 tcagctatga agaagcagac atagaaaagt atatctttgt ggccgtctta gacaagcgca
     7741 catcacgcat ttgtcaagaa catgacaatc aggtctatga tagggataag gctgtccctg
     7801 gtgtcaattg tccacctatg cacccttggt gtaggtctac tactgtcgca tacgacgagg
     7861 acgcagacta cagcaagttg aagcgcagag caaggaatcc agtgacaggt aagaccgagc
     7921 tggtgcctgc tgatatgact tataaagagt ggtatagcaa gtatgttgcg aaagacgggg
     7981 aaaaggtgta taatcaagat acaagagaag ccaaggcgaa attttatagc gaacaactat
     8041 tgtccaaaat ttcaggagtt gagccaaaaa ttacaagtga tatgcaacgt atcgcaggag
     8101 aaaacaaatt ggcaggtctt gaatttagga agaaaacagt tgagtcgtta tcacgtaaaa
     8161 ttattgcaga tagcctagtt gaaaatataa gtttgtcaaa agccgtgagt aagattaatg
     8221 acgccttaag gtacacaact attttcgatt ccgatacttt tacagaagag tatttgaaga
     8281 tgaaacagaa gcttgtcgca gaaggttata aaattgtaaa agtaaaaaac acttggttag
     8341 tagatggacc atacaaaggt gtgaatacag tcgttgaaaa agatggtatc aactttgaaa
     8401 tgcagtatca tactcaggaa agtttcgact taaaaaatgg ttcattacat gaactctatg
     8461 agaagtatcg tgatacgaat acatctgatc tagaacgcat gaaattattt aaggaaatgc
     8521 ttgatttaag caatgggctt gagattccta aaaatataga gagggtgaag tgatgtgaaa
     8581 gatattaaat actaccgcac aacgacgaac aatgctcaag tacttcgttt gattgatggt
     8641 gtcatgcaag tttttgacat tgaaaaaaag tgggttaata gcatagattg gtttaataaa
     8701 atctttttta atgactttac ggattttgaa gaaatttcag aaaatgatgc atttacttat
     8761 attgacagga tggtagcggc atgattgata ttgctttggc tatcgctaaa aaagcacatg
     8821 cagggcaggt agataaagcg gatgttgatt acatacagca tcctctctat gtggccagtc
     8881 aagtcaaaac tgaacaagaa aaagctgtcg ctcttttaca tgatgtgatt gaggatagcg
     8941 atataactgc tgccgattta ttggcgtctg gtttgtcaaa tgaagttgtt acagcggtac
     9001 aaattttgac aaagaaaaaa ggtcaaagtt atcaagaata tcttgggaaa gtaaaatcaa
     9061 ataatttagc aagagttgta aaacttgcag atttgaaaca caactcagat ttatcacgtt
     9121 tgaaatctgt taccaataca gactacgagc gtgttaaaaa atataaaaat gcaatttatt
     9181 acttaagcac ctagagaaat ctaagtgctt ttttcgtacc cagaaaggat tgaaaatgga
     9241 taaagcaaaa attgggataa ctaacgtaga attttcagga acaggcggaa ttgaatcagc
     9301 gacagtgaaa ttagagttaa atatttatgg ggcggatacg ttcagcgcga ttgagttact
     9361 acctaaaata ttaaccgaca ttcattcatt atcgtatgaa gttgattgat tgtgacatta
     9421 aaaggagtaa gatatgttta tatgggattg ggtatcaatc gctttcgggt ggttggtatt
     9481 tttgtttttg atgctgatta tcatagcatt tgtaaaaaaa gtgattgaag aaaatacaaa
     9541 ataatctaac cgtatggaat cccgtacggt ttttatattg tccaaactgt gccgatgaca
     9601 ttaaaagctg cactgttccg tcgccggacg taaagcgaga ttatcgagtg gcgacgtaat
     9661 cgctggagga caattatgtc agaagaaatc aatgcaactg tatctattga atcaactgag
     9721 actgtcgaca ctcaaggaaa tgttgattca gtgcaggaag aaaagcacga acgaactttc
     9781 actcgtgctg aaatcggtaa gatgctatct gccgagcgct ctaaatggga agctgagcaa
     9841 gaagccaagg aaaacgaagc taagaagctt gctaagatga acgctgatga aaaacagaaa
     9901 tatcagttgg atcagcgtga gcaagaacta gctgaccgtg aaaaggctat tgctcgtaag
     9961 gaattgaccg cagaggctaa agcaatgcta agtgaacgtg acttacctgt tgagttagta
    10021 aatgtagtcg atttgacaaa cgcagagacg gtatctgaat ctatcacctc tatccaaaaa
    10081 gcatgggaag agtcagttca gaagggagtc tctgaacgta tgaaaggtag tgcacctatc
    10141 aaaaatgcac aaacagtcca gcaagaagtc acggaaaaat ggcgtaaaga cttcttgtaa
    10201 taaaagaaaa gaggaaaaat aaatggcatt tgaagaatta aacacagcag aatcacgcaa
    10261 gaaacatctt gggattattg aggatgtact tgcagtaaat tcatattcaa caccacttgt
    10321 gacatcaagc gatgcagtaa ccttgcaagg tcgctctttt acagtagcaa ctggtaacac
    10381 aacagagttg aaagactaca aacgtaacaa agacaacgaa tttgatcacg ttgaagttga
    10441 agaaaaggtt tatacccttg atgaagaaaa atactggggt cgtttcgtag atcaattgga
    10501 cgaacgtgac tctaatggtc aagtgaatat caattatgtt attgcccgtc aagcggcaga
    10561 agtagtagct ccatatcttg atgaactacg ttttagtgca gcgcttggta atgttagcga
    10621 caacgttact atgggcaaag aagcaggagc gaacaacgca tacaacgcaa ttcttgatgt
    10681 ttctgagaaa cttgatgagc ttggaattac aaaagagcgc ttgctttttg taacacctaa
    10741 attctacaaa gcgatcaagt ctgaaatcgt tcgtctacca catggtgacg cagataagaa
    10801 agtccttgga aaaggatatg ttggtgaatt ggatgattac acagtctata aagttccttc
    10861 taaattcttg aaaggtgtta atgcccttgc tactgctcca ggtgttgtta catctccagt
    10921 acaagtagac aatactaagt acaacgataa cattccaggt cgatttggtg aattggtaga
    10981 gcaattactt tacactggtg catttgtgct tgaacacttc aaaaaataca tcatcacaat
    11041 tgcagattct aagcctgctg ctaaaaaatc agcccaaggc aagacagtga accgtgctaa
    11101 agcgtggaag actggaacag cctataaaga aggtgataca gtaacgcatg aagataaagt
    11161 ctatgttgct atcaaagaca tcactagctc aaccaatgca ccaggctctg actcagctaa
    11221 ctggaaagaa aaaactggta agaaataggt cttagttatg aaatttaaaa tcaaacaaga
    11281 tttctatgat tgggaatcaa atgtgaaacg actggcagga gaggaacttg agattactga
    11341 ggagcgctat gctgagctgg ctgacaatat tgccagcaac ggtgtcgcta tctcagatgt
    11401 tcttgagaaa atcctccctg aacctgagtt cttagaagag gattgatatg tctatagagt
    11461 tgctgaagaa attaacaggc gaagaagata ctcagcttct catgttgttc caaacaaggg
    11521 ctacaaatct tatcttgtca gagactaatc gcacatcttt gacacctgct ttaagtcttt
    11581 taatacctga ggttgctatc gagctccaca accgctcagg agcggaagga gagcgttcta
    11641 gaaccgaagg tggtatagca gtagtctacg gagaaaatgg cctgtctacg ggtcttctac
    11701 agcgaatacg catgcacagg ctagcaaggg tggcaggtca tgtttttgaa gcagagtaga
    11761 ctgaaacctt atccaatgcg acggtttgaa aagactgtca ctgaggaagg tgtcgcgaaa
    11821 gaagggtatg ccaaggaagc tgagacagtc cgccttgaat tgtggccagc tagtagcaag
    11881 ttacaatctg aattgtatgg cgagcgtgtc aatgatattt tgaacgcaaa tgccaacaag
    11941 tcagctacta tcaaagtgaa agatggtgtg tgtatcgata gccagacaga agtgactcac
    12001 agggttattt ctaaaaaggt ctacacacat catcaagttt tggagttaga gcgtgtcagg
    12061 gctactaggg gcagataggc ttatagctaa atgtagacga ttggctagta aaaaaactgg
    12121 cgaggatatc gtcttacgtg cggtacacaa tgctactata aaggttgtcc aagcagatgc
    12181 aagaagactc gcaccagcga gagatggaga gcttgtaatt agtatcaaaa ctagagcaaa
    12241 aatggacgga gataaggcta taggcgaagt ttacaccaac cttaaatacg ctccttacgt
    12301 tgagtttgga acaggaccaa taggacaagc taaccattcg ggtatctctc cagaggtcag
    12361 cgtgacttac aagtctaatc cttggtatgt gcatgaagac caaataaatg taggaccgta
    12421 ccactttcaa aagattgggg agttctacaa gatgtatggt caacctgccc agccttatct
    12481 ttatccagct ttgagagaca atcaagagcg tgtgtctaag aatatttcga attatgtccg
    12541 tagaaagata agagaacaaa taaaatgatt aatatcaagc ctgttattta taaagaattg
    12601 caaaaggttg cagataatgt gaccgatact tatcctagcg attgggagac tttcccagtc
    12661 gttatttttt tagaagaaca aaacaagccg ggtgattggt ttgatgacca ggaacaaaaa
    12721 tcatctatcc gctataaggt ggatatcttt gatgatacca gcactagtga gttagctgtt
    12781 aaaatcaatc agatttttga gtctttaggt ttgcgaagaa ctgactgcca agacgtgcca
    12841 gacccgtctc atttgagaca taaggtcatg cgttttgaag gtgtcgttga tttagactca
    12901 gagcttgttt ttcaatttag aatggagaat taaacatgtt agcaaatgga attaaattgg
    12961 cctttagtga aacgaaaggc aattatcaga atcttgtagg gcttaaggaa gtacctgaat
    13021 ttggtatcga gcccgaaaaa gtagaaaata ctactcttgc agatacggtg aagaagtacg
    13081 agtttggtat cggggacgca ggagaacttg agtacaagtt cgcttataat aattcaagcg
    13141 caacagctcc ttaccgtgta ttacgtaagg cagcagatgg caagaaaaaa ctctactttg
    13201 aacaaaccta cccagacggt actaaggtca cttttgaagg tcaagtatcc gttaaactgg
    13261 gcggtggcgg cgttaatgcc gttatcgagt tcacacttaa gattgcattg cagtctgaat
    13321 tgacattcgt tgatggaatt ggaggttaat taaatggcgt taaaatacac aacttggaaa
    13381 gttactgacg aaaaagagtt gaagctacgt ttgacatctc atcaagctgc aactgtggaa
    13441 gaaaaaatcg gcatgaactt gttaaagatt ttcatgcctg aagctggcga agagttcact
    13501 ttaccgcctt tgaaagttat gctgttgtta gttcatggag ccttgcagca gtatgaacat
    13561 gggtattcct ttgaagatgt ctacgaccta tacgatgaat acgtggataa cggcggagac
    13621 caaacgacat tcatgacaga ggtgttgatg ccgctatttg aagtatcggg ttttactcca
    13681 cgaggaagca aggacaagaa aacttccaag aagaaaatga cagtagtcga gtaatcttaa
    13741 cggtaacgca gattattgag aggctttatc ctatgttttt agacatcggg ggcaagcctc
    13801 ttgatttttg ggatttaacg gtgcttgaaa tcagggaaat gatagaaagc tacaaccgtg
    13861 tcaaaatcca agagcgtaaa gaaaagatta ttgactcgta catactttcg cgaatgataa
    13921 ctaatcatgt ttccttatta ctgtccaatg acgctaagat tgttgagctt tgggaatatg
    13981 cgcctgagtt gtttgtagaa gaacagcaag cagtagaaca ggaacgacag agacaagcgc
    14041 ttttgttgca taaggaacgg atgcgtgatt ttgcagagag acataatcga aaaaggaagg
    14101 aggaagtaaa tggcaactct tgatgaattg aaggtcatga ttgacgctga gatagcgcct
    14161 ttcaggaaaa agatgaaaga agtcgagaat caggtcaaag gaacatctga ccaagtgaaa
    14221 aatgccactg ccaaagttcg tgaacagtcg aactcaatcg gtagtgcgtt tggtaagctg
    14281 gctaagtttg ctggttttgc aatccttggt aagaaattac ttgatgttgg aatgtattca
    14341 acgcagacag ctcttgaagt gtcagcgtct atgaaccaaa tcaagcgaca gatgggcgag
    14401 agttcgcaat ctttcttgaa atgggttaac gataacgcca acgctatgaa tatgggagtg
    14461 ggtgaggcga ccaactacgg tgcagtctat tcaaacttat tttctggatt tatcaaagat
    14521 accaacaagc taagcgccta tactgctaag atgttgcaga catcggcagt ggttgctgaa
    14581 ggttcagggc gcacgattac agacgttatg gagcggattc gctcaggtct actagggaac
    14641 accgaagcaa ttgaggacct aggaatcaac gtcaatgtgg ctatgattaa gtccactgaa
    14701 gcttttaaga agttcgcaaa cggacaaagc tggcaacaat tagactacca aacccagcaa
    14761 caaatccgcc ttatggctat tctggaacag gctacagcca agtatgggaa taccttgtct
    14821 aattctgtaa atggtcgtat cagcctattt aagtcgctga tgaaggacgc agcattgaac
    14881 cttggtaact ctatgttacc gattatcaat gccattatgc ctgtcttgaa ctcttttgct
    14941 atggttttga agaacgttac tgctaaactc gctgagttta ttgctttgat gttcaacaag
    15001 aaagcaacag tgaaagatgg tgttggtgga gcagttggag acatgggtaa cgccatgaag
    15061 gatgctgcag gcggagcagg agaccttgct gacgcagtag acgacgctgg agattcagca
    15121 ggaggacttg ctgacaatct tggagactca gccaaaaacg ctaagaaagc tgctaaagag
    15181 ttgctaggtc ttttgggatt tgatgagatt aacatcttgc aaaaaccaaa agatgacgac
    15241 gcaggcggtt ctggaggcgg tggcaaaggt ggtaaaggaa agggaggcgg tggcggacct
    15301 ttcaaagaca tcttgccaga agtcgagttg actgacatgg acaacaaatt caagagcatt
    15361 tttgatggtc ttggagataa gctcaaaggg ttgtttgacc tcttcaagaa aggttttgat
    15421 gcagcattta gaccagaagg tataaaacgc attaagactg ccttagacca aatagctaag
    15481 acaatgggag aaatcgccac tgacccaagg gttgtgaatg cctttaaccg aatggctgag
    15541 aaaattgctt atgctttagg gcaagtgaca ggctcaatag ctactatcgg gctaggtatc
    15601 ggtgttttcc ttgccgaaag tattgcaaat ggccttggaa ggcaaaaaga acgcattacc
    15661 agggcgctag tcgctttgtt tgataatgtt ggtaaccttt ccgaggcagt aggaaacata
    15721 gctcaggact tttctagtgc tttctacgac gtcattacct caactggtgc ggttcgtatc
    15781 ggtagcgcta ttgtgtcaac tctgttgagt ttgacatcta ccattgttga agttggtagt
    15841 aaattagcag gaagtttgtt taaaggtttt gaaaaagtcg ttgtgacaag cgctcctaaa
    15901 atttcatcag tcttccaaag tttattagat actgttgcgc ctgtatttga gagcattgaa
    15961 aggtctgtta acaaatttgg cgatggctta agtcgtgttt atgatgaaca tgtagtccct
    16021 gctattaact ctattgctaa tgcttttaat gggctaattg acattattca gattctctgg
    16081 gagaattcct ggcaaccttt tgctgagttt ttatcaggag tattcggtgt tagtattgaa
    16141 ggaatttcag atttattagg aggtggcctt ttagccactt tgggactatt ggcgtatgct
    16201 attaagttag tggcagatgg tttcaccgtt ttttctgact ggtgtaaaga aaacaaagaa
    16261 cctatcgtag ctttgataac aacttggcaa acgattaatt tcttatcatg ggcagaacaa
    16321 gctggaggac ttgcaggagc attcagcttg ttaggtagta aggtctcttt gattgttgga
    16381 ggaattaaga atctaggtct tgctattaaa gcattgacat ttgataagtt ggtcagtttt
    16441 gctgaaacaa tctatttgaa caccttatat gcaaaagatt ttgtggtcaa ttcaggtaaa
    16501 acaattgcac agctaggaaa aactgcttta gaacttggta aatcagctct agcatggact
    16561 gctcatgcag cgaaaatggg attagcaacc gcggcggaat ttgcacattc tgttgcagca
    16621 ggagtcgcta cagctgcaac atgggctttt aatgcagcgt tagcagtttt gacaagtcca
    16681 ataacatgga ttattgcagc aatcgcagcc ttaattgcta tcggtgtttt gctctaccaa
    16741 aattgggaca ctgttgttga gtttgctaaa actgcatggc aaggactatg tgattttatc
    16801 agtggtattt gtcaagcgat tggcgaattt ttcagcggtc tatggacgaa actacaagaa
    16861 atctttgagc cgataggtca atggtttggc gagaaattcc agcaagcatg ggacgctatt
    16921 ggaaatatat ttggcaactt aggctcttgg tttggtggcc gttggaatga ttctaaaaac
    16981 gcacttgccg aagcaaatac ttggcttggt gataagttca agtcaggtcg ggataaagtg
    17041 aactcagctt ttgaaaaagt tggctcttgg ttcggtgacc gttggaatga tatcaaagat
    17101 ggagtgaaag aagctgatac atggtttgga gagaaatttg agagtgcaaa aaagaaaact
    17161 cagaatcctt tccaaaaaat cggttcttgg tttggtgata gatggaaaga tatgcaagat
    17221 gccttgaaag aaatccccaa ctggttcaag aatctgttta atgatgcaat ggataatgct
    17281 aaaaacatcg ttaaaagtgg tatcgataaa ctaaaaagct tctttaactt tgattggagc
    17341 ttaccaaaaa tcaaactccc tcactttaat atatctggta gcttcagctt gatgcctcct
    17401 agaattccat cattctctgt agattggtat gcacgaggcg gtgtattcaa ctctcctagc
    17461 atcattgggg tcggagaagc tggtcaagaa gcggtaatgc ctcttgaacg gaatacagga
    17521 tggatttcta tcttggctca aaaactggct gaaagaatgc ctgctaataa tgtacctaca
    17581 ggttattcat taccggctgg cgacatcgtt atccaaatcg caggccatga gttcggacgg
    17641 gtagctatcc aagaaatcaa caaggaacat gaacgagcag gtcaaacctt gctcaagatt
    17701 taggaggtta aatggcacaa ttgacaatca atggggtggc tgtgaagcct cccaaatctt
    17761 ttcaagtcgg tattcaagat atcgatggag agacagggcg taatgccaat ggcgacatgg
    17821 tgcgtgaccg tatcacgacc aaacgcaaac tagactgtga atggggtatg atgactcagg
    17881 gagaaataag tcagctttta catgctgtat catctgaatt ttttgaggta tcttatccag
    17941 accccatgga tggccaagtc acaaagactt tctatgtcgg tgctaggaca gctcctagct
    18001 atacctttac tgagaagttt aaaccttggt ctggcgctaa atttaatctg gtagagaggt
    18061 aagaaaatgg acgctttaac tagacgacaa tttgacagat ctatgtttgc caaggaaagg
    18121 acgctggcta ttcgtgttgg tgaatatgct tcacgggata tcaaagaggc tagttttgag
    18181 tatggctaca tcaagggcga tacttataag cctggtggaa cctgcgctgg tagcggtaaa
    18241 attaccttta ccagtatcat taccacgttc aataagctgg ataccctgca ccctgagatt
    18301 ggtctactgg ttggggatac ctaccagtgg gtcaagatgg gggaatactt catcaacgat
    18361 attgagattg accgaaaccg aaacactacc acgcttgaac ttatggacgg tatgtttaag
    18421 ctcaatcgtg agtacgtgac ggacttgcat ttcccagctg aagtacgaga ggttattcag
    18481 gaaatctgcc tgaaaacagg cattgagtta gcgaatgact atttcggaat cagcgcgatg
    18541 cgttatcata ttgagcaagt tcctgagggc aagaaacttt cctttaggga tatgctgagc
    18601 gctatgactc agatgattgg gatgtcttgt ttcttcaaca gagaaggcaa gatggaaatc
    18661 cgtgatttga ctgagtccaa tatcacgatc aacgctgaca gttacttctt gcatggcttg
    18721 accaagagtg agattgagta tcagatagct ggtatcactt gtaagacgga caagaagtct
    18781 ctgacggtcg gtatgaagac gggccggtct ttggaactgg acaatgtctt catgacccag
    18841 agcgctttaa atgacctgta ttacaaactg aaaaacctaa cttactatcc gtataatctc
    18901 aactaccaag ggcatttact gcttgaggtc gggcagtggg taaccattca gaccaataag
    18961 aaagagacct ttaaagttcc tgtgttaagt cagagcttta cttttaaagg tggtctgaga
    19021 gggcgtatca gcgcagatag taaggctgga aacgatactc agtattctta cgagggtacg
    19081 attaccaagc atattaagca acaaggtggc attgaagcga aaatccaagc gcagattgaa
    19141 gcaacagata aagattttga ccaaaaggtc gacaaaatca aaaaagactt taacgatcaa
    19201 gtagaacttg ccaaggccaa ggcggaagaa gtcaagagag aactgtctga cactatcaat
    19261 cagcgattta atagctttga caacgggcca ttgaaagaaa ctaagcgcaa ggctgaggaa
    19321 gctttgcgaa atgctggcgc aagtaccctg cttgcacagg aagctaagcg gattggactg
    19381 gattctgtcg ctagacttga agcgtttaag tcgcagacta cgagcgcaca aacggctctg
    19441 tcgggtgact tggacgctct gaaacggact atcgcgaatg atattcgacc gaagcaagca
    19501 caggctgaag ctgagattgc caagcaagtt gaagcactta gccggactaa aaatgaactg
    19561 gctggcgcaa gtagcttact cgctcaggaa gctaagcgga ttgagctgga ttctgtcgct
    19621 agacttgaag cgtttaagtc gcagactacg agcgctcaga cggctttgtc aggtgacttg
    19681 gatgttctaa aacgaactat cgcaaacgat attcgaccga agcaagcaca ggctgaagct
    19741 gagattgcca agcaagttga agcacttagc cggactaaaa atgaactggc tggcgtgaag
    19801 tcagcgcaag cgacgtatga ggagacgacg actcgtagac tttcagaact gaccaacttg
    19861 gccaatggta aagccagcaa gtcagaactc acgcaaacag ctgaggagct ggctagtcgg
    19921 atagcgagtg tgcaggtcgg gggtaggaat tatatccggg gtacaaagcg catgatgcta
    19981 gccagaggat tgtgggcatc aggtactttt agaccgtcag gcgctgggac ggcaaagacg
    20041 attgatgtat cagacagtcc agcaactggc tttgataaag cgatacgatt gacctcaagc
    20101 aatgctagag accaaatagg tattgctcaa gatggatttt atatctcgca aggcacatac
    20161 acgatgtctt gttgggtcaa aggcagaaga ggtcaaaagg tcaagctaca aacttattgg
    20221 caagtcaatg ataattcggg tatttcgccc atctttacat taaaggatga aaattggaca
    20281 aagctatcgt ttactagcgc tagaaatagg gctggagtcg catcaattgg ctatgtgtat
    20341 ctcgtaaatg ctgaggtcgg agaatattta gatgttcttg cgccccagct ggaagacgga
    20401 agtttggcaa caagctcaaa agaagctcct gaagatatag aaggtcagat ttcaacagtt
    20461 gaatcgacct tcaaacagcg tgccaactcg ctcgaagctg gtgtaagccg tctgactgaa
    20521 gggcttagaa ctaaagcgga tatcagctca ctcaatgtga ctgctgagaa tatccggcaa
    20581 tctgtgaaga gtcttgagac agacacgcag aacaagctaa atcagaagtt gagtcaggct
    20641 gaatttgagg tgcgagctgg ctctatccat caggaaatcc tgaacgcaac caaggataaa
    20701 gccagcaagt cagaactcac gcagacagct gaggagctgg ctagtcggat tgcgagtgtg
    20761 caggcatccg gtcgaaatct attcttgaac tcactattca agcaggatat ttcaaaaaca
    20821 ggaatttgga caacgagtac atatacggct gctatcgata gcgaaagtaa gtatcttgga
    20881 cataaggctc ttaaaattat aggtctgaat ccatctggcc gtgatggagg gaatcccaag
    20941 gttacttacc cagctctggg tcaattcggg aaagtaattc ccggaagtac gactaatcaa
    21001 gatgtaacca ttagttttta tgctaaggca aataaaaatg gaataatgct aagatctcga
    21061 ttagggaata tcggatataa aactggaaat gtgacattgt cgacagaaat taaacgatat
    21121 gttgtccata ttccaaaagg ttggacaaac gaatccaagc agaccacaaa tgaatggttg
    21181 ttcaatttca accaggaagg aaccgtttgg atttggatgc cgaagtttga aataagcgat
    21241 gtagatactt cttattcaga agctcctgaa gatatagaag gtcagatttc aacagttgaa
    21301 tcgaccttca aacaacgagc caactcactc gacgctggtg taagccgtct gactgaaggc
    21361 cttagaacca aagccgatat cagctcactc aatgtgactg ctgaaaatat ccggcaatct
    21421 gtgaagagtc ttgagacaga cacgcagaac aaactaaatc agaagttgag tcaggctgaa
    21481 tttgaggtgc gagctggctc tatccgtcag gaaatcctga acgcaaccaa ggataaagca
    21541 gataagactt tagttgtatc tgaagctggg aaattgcgtg aagaattttc aaaaatgaag
    21601 gtgggaggcc ggaatctatg gataaaatcc aagacggttg gagctgtaat tgaaaaatta
    21661 cctgaaaacc acgtcacagg tcaaaaagaa tgctataggc tagagaacaa ctctacttta
    21721 acgttcaacc ttgaaccaga tttcagctca aggttgtacc aaaaagttac ttttagcgct
    21781 tggatcaagt acgaaaatgt agtccaaggt cgaaattttt ggaatgtatt taattgcttc
    21841 aaacattatc tttttagaaa aaatagtgag accggagtac agagtggtcc agattatgct
    21901 acgcttggta tgtataaagg ttcggcagat tggaaatata ttacattcac ttatgactac
    21961 tctgaaaaaa caaattttga tcaattgaag acatcattgc gattcaatct tgaaggtgct
    22021 acaagcggta cagcttgggt aacaggaatc aaggttgaaa tcggtagtgt ggcgacggac
    22081 tggagtcctg cgcctgagga cgctgatggt ctcatcactg aagctaaggc tacctttgag
    22141 cggacagctc agggcttgcg aaccgattta tcagctattc aggaatatgt aaataaaaac
    22201 ggtcagcgac aggaagccct acagcgctat actcgtgagg agagcacgag acaagcgaca
    22261 gcagtccgtg agctggtcaa tcgtgatttc gttggtaagg ttacttatca agaagatgtt
    22321 aagggtatca atcagaggat tgaagctgtt aaaactagtg cgaataaaga catcgctagt
    22381 caaatcgcta gctatcgtca atctgtagat ggtaagttca cggatatttc aagtcagata
    22441 actacttata agcaagatgt gggcggtcaa atcagtggtc tatcaaatag acttacaagc
    22501 agtgagcaag gagccactac tcagatttca aatatttcaa atcggataaa cagtaataaa
    22561 caaggcacag ataatcagat ttcaaattta aagactcagg tcgctacaaa caaggataat
    22621 gctgaacgac aaatgggtag aatatctgat caggtttctg caaacaaagc gaatgctgat
    22681 agtcaatttg cgaatgtgac caatcaacta gcgcgaaaag tagagactac tgacttccag
    22741 cgtgttaagg aaaccagtaa actttacgag cggattttgg gcaatactga aaatggaatt
    22801 gcggataagg ttgctcgcat ggctctgacc aatcaactgt ttcaggttga ggttgggaaa
    22861 tatagtgtaa gcggccctaa cctcattaag aatagtgatt ttaaaaatgc tacgaatgaa
    22921 tggggctcaa ctcaaaattt aggaagattg gttaagcata gcttttatca caacgggcag
    22981 aaagacctta tgcgtttaag taatgcaact aaaaacgaaa actttttgta tagtcaccgt
    23041 tttaatcttg aacgaaatac tgactatgta ctgaatttta gaggatttaa caacagtgct
    23101 ctcgcaagct atgatgttta tattttggga cgaagagcag gcgagagcga tggattcaca
    23161 atcgttaaga aagttgttag cagcaagaaa ctatctacct ctagatgcga agatgtctca
    23221 gtaactttta attccggaga aatggataat gcttacattc gttttgataa caatggctca
    23281 tcatcaggaa cagctgattt gtatattaca gaagttgact tgtacaaagg ttataaacct
    23341 agaacatggc aaccacatcc agaagatgtg gtcgcagatg cgaataagaa gcttgaagcc
    23401 acgcaaacaa aaatgactct actaactggc tcatgggcag ttcaaaacat caactcggct
    23461 ggagatatca tctctggaat caatcttggc gccaatggac ataaccgctt tgttgggaaa
    23521 ttgacccaca tcactggaga gaccctgatt gacagagcag tcatcaagtc tgccatggtt
    23581 gataagttga aaacgggcaa ttttgaagct ggttcggtca cgactacgat attagaagct
    23641 gaagcggtaa ctgctgagaa gttgaaagtt gacaatgcgc ttattaaaaa attaactgca
    23701 aatgatgctt ttattgacca actgacatct aaacgtatct tctctactaa ggttgagtcc
    23761 gtcatttcta gttcaacctt cctagaagcc tatcaaggtc gaattggtgg attcacactt
    23821 ggtcaatttg accagggtgg cggtcgctgg atttcaggtg tcaatcagtt ctctgttggt
    23881 atggggaatg gtgccgggca tggagtccgg acagccttct gggcgaactg gggaaataat
    23941 tggaactatg ccggacctaa agcatggaac gtcaatactg atgggaaaat gtactgtagg
    24001 aatgaagtcg gtttttatga tcaagtggat ttttcgaatt catcgagagc aaacttctat
    24061 gggaatacta ctttttctcg ttctcctgtg ttttcaaatg gtatcgaact tggaagtaaa
    24121 gatgtgcttg gtgatggttg gaatcccaaa ggcggaagga atgcggttgt ttggtggaat
    24181 caggtcggta gcggtagcgt gaagtattgg atggaacaaa aatcagacag acgcttaaaa
    24241 gagaacatca cagatacagc tgtgaaagcc ttggataaaa tcaacagatt aagaatggtt
    24301 gcatttgatt tcatcgaaag taagaaacat gaggagattg gtctaatagc tcaagaggct
    24361 gaaaccatcg ttccaagaat tgtctcacga gatcctgaga atccagatgg ctatctacat
    24421 atcgactata ccgctttagt tccttactta atcaaggcta ttcaagaatt aaatcaaaaa
    24481 atagaaaaaa tggagaaaac aatagcatga ataacaacat gttgaccaat atcgcactta
    24541 aagcaattta agagcttgct cttgaaaata gaaaacgaac acacagattg gagaacttag
    24601 aaaatgaaca cagaacagct taaccaagcc ttacaaatga caattagtga aatgtcaaca
    24661 acttcaacaa attcgatgat tacaagtaat atcttgagta ttcagttgaa tgagcaaagg
    24721 gaagagaatc aaagacttca agcacgagtg gatgagctgg aagctctgct tgatgaacaa
    24781 actaaaccag cagacaaagg agaatagaca tggcagaaac aattcaaaac acagataact
    24841 tactagacct tacaaaaatc acagaaccat ttgatcttgc gagcgctttg cgctacatga
    24901 aagaaaatgg agagttcatt cgttgcaaga atgtaagcga tgacttctat atgtatcgtg
    24961 atgttcaaaa acgtcctgtg atcgtaaatg gccgtcgcca attaaaggat gttgaaaccg
    25021 tttgggcgtt caaccagtgg ggtggtacaa tcgcaacaat caacgtagcc gttctgttga
    25081 atcatgaatt ctatatcatg aaatttgatg cagagggcaa tcctgactgg acggatccaa
    25141 cggtaaaacc taaagaatag gaggtgtgta tgccaattga agaagctgaa aaaatcgctc
    25201 aaagtcaggt agcttgggcg attttgttta tcttgctttt ctttattatc attcgatatc
    25261 ttatcaagac ttcggacaag cgagagaaga agattatgga tttgcacgag caatcaaagg
    25321 ccgactctaa tagacgagaa gagcgtttga tgactcacct agaaaagacc actacagaat
    25381 taaccacaat cactcacgcg gtcggagaca ttcaaaaaga aatggttcgc atgaacgacc
    25441 gcatggaaga aatcgaaaaa ggagaataac aaatgcaaca aattactgaa atcattacta
    25501 atggagcaat cagcatccta gtcgttttgg caggggttgt agttagggca gtcaaggact
    25561 acctggttca aaaaggtgga gaaaagacca tcaagattgt tgaaatcttg gccaaaaatg
    25621 cagtaaatgc cgtggagcag gtagctgctg aaactggcta caagggagat gaaaaactgg
    25681 cacaggctcg cgctaaaatt cgtgctgagc ttacaaaata caatattagc atgactgaca
    25741 aagacttaga caccttcgta gagtcagcag tgaaacagat gaacgacgct tggaaaggac
    25801 aagagtaatg gatatcgata caagcagact acgaacaggc ttgccccagg ttggggtgca
    25861 gccttatcga caagtacatg cccactcaac aggcaaccgt aactcaaccg ctcaaaatga
    25921 agcggattat cactggcgga aagacccaga attaggtttt ttctcgcacg ttgttgggaa
    25981 cggtcgcatc atgcaagtag gtcctgtgaa caacggaagt tgggatgttg ggggcggttg
    26041 gaatgctgag agttatgcag cggttgaact gattgaaagc cattcaacta aggaagagtt
    26101 tatggctgac tatcgcctct atatcgaatt gctacgcaat ctagcggacg aagcaggctt
    26161 gccgaagact cttgatacag acgacttggc aggtatcaag acgcatgaat actgtaccaa
    26221 taaccaacca aacaaccact cagaccatgt ggatccatat ccatatcttg caagttgggg
    26281 cattagccgt gaacagttta agcaagacat cgaaaacggc ttgagcgctg caacaggctg
    26341 gcagaaaaat ggcactggct actggtacgt acactcagac ggcttttatc caaaagataa
    26401 gtttgagaaa atcaacggta cctggtatta tttcgatggc tcaggctata tgctttcaga
    26461 ccgctggaag aagcacacag acggtaattg gtactggttc gacaactcag gcgaaatggc
    26521 tacaggctgg aagaaaatcg ctgagaagtg gtactatttt gatgtagaag gtgccatgaa
    26581 gacaggctgg gtcaagtaca aggacacctg gtactactta gacgctaaag aaggcgccat
    26641 ggtatcaaat gcctttatcc agtcagcgga cggaacaggc tggtactacc tcaaaccaga
    26701 cggtagcatg gcagacaagc cagagttcac agtagagcca gatggcttga ttacagttaa
    26761 ataaatagaa aggaaacttt ctaaattgtt ctttcaccgc aggctcaggc ttgcggtttt
    26821 tttgtttgct caaaaggggc aaaaaagggg caaaagtgtc gtaaatctct gtaaaatgat
    26881 gtaaaaacat ttatttaaaa gctcaaaata tagctgtttt aaaaggtatt gtaagatata
    26941 gtaaaacgat gtaaaggtat ttttaaatgc ggatgaatta taaaaccacg aattctatgt
    27001 gactcgtggt tcttttttat aaactagtag agtgttttgg ttgtactttt tgttcagggt
    27061 caatgtagtt gatagcgttg ttgacagcag ttggagcttc tccgaggcct gtcgcaatca
    27121 gatcaatttt tccgtcatag tagcagcagt caccgatagc atagatacct gcttggctgg
    27181 attcctgttt gctgttgacg ataatcttgt gacggttgag gtcgagcccc cagtttttaa
    27241 ggttaccgac agaagatttg aaaccatagt tgacaaagag gtggtctagg tcaatggttt
    27301 cagtttcatc agatttgact tttgtgattt caagtttatc aagtgttttt ccatttccaa
    27361 ggagttggct aggggcgaat ggtgtcttga tggttacaga tgattcttgc aaggcttgaa
    27421 cactgtgttc caaggcacgg aaattatctc tgcggtgaac aagggtagtt ggtgcgattt
    27481 tttcaaaagc caaagcccaa tccacagccg agtctccccc accaagaatc gtcactttct
    27541 taccagcgta ttgctgaatg ttagaaacgt ggtagtggat attttcatag ccctcaaccc
    27601 cttcaagttc cagcggacgt ggtttgaagg caccgccacc catagcgatg ataactgttt
    27661 tagtcaggtg acttccttta gaagttgtga tggcaaattc ttcttcttgt ttgtcaatct
    27721 caagaaccgt ttcattgaga tgaatagggg tatcaaatcc atttagctgt tcaatcaagc
    27781 ggttagtcaa ctcttctcca gtcaggtttg ggaagcctgg tacgtctagg atttcctttt
    27841 cagggtagag aatagcaggt tgtccaccta gctggggaag agagtcgatg atttgaacct
    27901 tggcttggcg taggtgggca taaaaggctg caaaaagccc gacaggacca ccacccacaa
    27961 tggtaatatc atagagttga gacatagttt ctcctttgtt ttttctagtc agtttatttt
    28021 atcatatttt tgttcaatgt aaaatggact aggataggga aaaaaatgcc agaaaatggt
    28081 ataataggac gaaagtgttt tgatagggag gatatcaggg atgaattttc aacaattatc
    28141 caatctgcaa tattggacca gtttgtttgc aagtccatgg acgatagcta tcaatctgat
    28201 tgatattttg attgttgctt atattttata ccattttaca aaagctattg caggaaccaa
    28261 gattatgatt ttggtacgtg gagttttggt gtttatttta gctcaaatcc ttgcaaatat
    28321 gattggtttg actacgattt cttggttaat caatcaaatt attacttatg gggttattgc
    28381 ggcggttgtt atcttctctc cagagattcg gactggtttg gaacgtttgg gaagagcgac
    28441 agattttttt tccaatgccc ctattagtgc tgaggaacag atgattcgtg cctttgttaa
    28501 gtctgttgaa tacatgagtc ctcgtaaaat cggggccttg gttgctattc agcgtgtacg
    28561 taccttgcag gagtatattt cgacaggaat tcccttggat gctaagattt ctgcagaact
    28621 tctcattaac atttttattc ccaacactcc cctacatgat ggtgcggtga ttatcaaaga
    28681 agaacgtatc gctgtgacgt ctgcctatct gcccttgaca aaaaacacag ggatttccaa
    28741 ggaatttggg accagacacc gggcggctat cggtttatca gaagtctcag atgccttgac
    28801 ttttgtcgta tcagaggaaa cgggaggaat ttcgataacc tataatggaa ggtttaagca
    28861 caacctaaca cttgatgaat ttgaaacaga attacgtgaa atcttacttc caaaagagga
    28921 agtgggtctt agttttaaag aacgattgct aggaggatgg aaacatgaaa aaaaatagtt
    28981 tatatatcat atcctcactc ttttttgctt gtgtcttatt tgtctatgct acggcgacga
    29041 attttcaaaa cagtaccagt gctaggcagg taaaaacgga aacctatact aatacagtaa
    29101 caaatgtccc tattgacata cgctataata gtgataagta ttttattagc ggttttgctt
    29161 cagaagtatc agtggtcttg actggggcaa atcgcctatc gctagctagt gaaatgcaag
    29221 aaagtacacg taaattcaag gttactgctg acctaacaga tgccggtgtt ggaacgattg
    29281 aagttccttt gagcattgaa gatttaccca atgggctgac cgctgtggcg actccgcaaa
    29341 aaattacagt caagattggt aagaaggctc agaaggataa ggtaaagatt gtaccagaga
    29401 ttgaccctag tcaaattgat agtcgggtac aaattgaaaa tgtcatggtg tcagataaag
    29461 aagtgtctat tacgagtgac caagagacat tggatagaat tgataagatt atcgctgtct
    29521 tgccaactag cgaacgcata acaggtaatt acagtggttc agtacctttg caggcaatcg
    29581 accgcaatgg tgttgtctta ccggcagtta tcactccgtt tgatacaata atgaaggtga
    29641 ctacaaaacc agtagcacca agttcaagca catcaaattc aagtacaagc agttcatcgg
    29701 agacatcttc gtcaacgaaa gcaactagtt caaaaacgaa ttaaaaatag aaaaggattt
    29761 tataacaatg ggtaaatatt ttgggactga tggagtccgt ggagaagcta acctagaact
    29821 aacaccagaa ttagccttta aactaggacg ttttggaggc tatgttctta gtcaacatga
    29881 aacggaagcg ccgaaagtct ttgtaggacg tgacacacgt atttcagggg aaatgttgga
    29941 atcggccttg gtggcaggtc tcctttcagt agggattcac gtatacaaac ttggtgtcct
    30001 tgcaacacca gcagtagctt acttggttga aactgaagga gcaagtgccg gtgtcatgat
    30061 ttctgctagc cacaacccag cccttgataa cggaatcaag ttctttggcg gtgatggctt
    30121 caaactagat gatgaaaaag aagcagaaat tgaagccttg ctagatgctg aggaagacac
    30181 tcttcctcgt ccaagtgcag aaggcttagg aattttggta gattatccag aaggcttgcg
    30241 taagtatgaa ggataccttg tttcaactgg aactcctctt gatggaatga aggttgcctt
    30301 ggatacagct aatggagcag cttctaccag tgcccgtcaa atctttgcag accttggtgc
    30361 ccaattgacg gttatcgggg aaacaccaga cggtcttaac atcaacctta atgttggttc
    30421 aacacatcca gaagcccttc aagaagtggt caaagaaagt gggtcagtta ttggtttggc
    30481 ctttgatgga gacagtgacc gcttgattgc tgttgatgag aatggtgaca tcgtcgatgg
    30541 tgacaagatt atgtacatca tcggaaaata cctttctgaa aaaggacaat tggctcaaaa
    30601 tacaattgtg acaactgtta tgtctaacct tggtttccac aaggccttga atcgcgaagg
    30661 tattaacaag gcagttactg cagttggtga ccgctacgtt gttgaagaaa tgagaaaatc
    30721 aggttacaac cttggtggtg aacagtctgg tcacgttatc ttgatggatt acaataccac
    30781 aggtgatggt caattatcag cagttcaatt gactaaaatc atgaaggaaa ctggtaagag
    30841 cttatcagag ttggcggcag aagtaacgat ttatccacaa aaattagtta atatccgagt
    30901 ggaaaacgtc atgaaggaaa aggccatgga agtgccagct atcaaggcca tcatcgagaa
    30961 gatggaagaa gaaatggcgg ggaacggccg tatccttgtt cgtccaagtg gaacagagcc
    31021 cctcttgcgt gttatggcag aagcgcctac aacagaagaa gtggactact atgttgatac
    31081 tatcacagat gtagttcgtg ctgaaattgg gattgactaa atcctagaaa actagatgaa
    31141 aataaaaaat tatggtacaa tagcttataa tattgtagcc gatggagaaa gacatgaatc
    31201 ttaaacgtga acaagaattt gttagtcagt atcattttga tgcacgtaat tttgaatggg
    31261 aaaatgaaaa tggagctcct gaaaccaagg tggatgtgaa tttccaattg ctccaacatg
    31321 accaagaaaa tcaagtgact tccttgattg tcatcttgag ctttatgatt gtatttgata
    31381 agtttgtcat tagtggaaca atctcacaag taaaccatat cgatggccgt attgtcaatg
    31441 aaccaagtga attgaaccaa gaagaggtgg aaaccttggc tcgtccatgt ttgaacatgc
    31501 tcaatcgttt gacttacgaa gtgactgaaa ttgcattaga cttaccagga attaatttgg
    31561 agttctaata tgaaattagc tgttttcaca gattcctctg cttatctcag tgcagagatc
    31621 ttgcaaagag aagacttgtt tgtcttggat attcctgtca atattgatgg tgaggaatat
    31681 gtcgaaggca tcaatctgtc tgctgaggaa ttttaccaaa aaatggctca ggcttctgaa
    31741 ttgcctaaga ccagtcaacc aagtattgcc aagttagatg aaatcttaac ttcgctcaaa
    31801 gaacaaggtt atacacatgc cttggggctt ttcctatctt ctggaatttc aggtttttac
    31861 caaaatatcc agtatatggt cgatgactat gagggcttaa ccattgcttt cccagacact
    31921 ttgattacaa gtgctcccct aggcatcatg gttgaaagcg tctttaattg gcgtgatcag
    31981 ggcgatgatt ttgccagcat tcaggataag ctagccattc aaatcagccg tacgtcagcc
    32041 tttatcatgg tagatgactt ggatcatttg gtaaaaggtg gacgcctttc aaatggggct
    32101 gccattttgg gcaatttgct tagcattaag ccaatccttt attttaacga tcaaggtgtg
    32161 attgaagttt acgaaaaagt tcgtactgaa aagaaggcca ccaaacgctt aattgaaatt
    32221 atcaaggaaa caacggcttc aggtcaatac cgggtcattg tcattcatgg gaatgctcct
    32281 gaaaaggctg aagaattgcg tcagcatttg cttgattttg gcttgggttc ggatgtttca
    32341 cttgctacat ttggtagtgt cattggaacg cacctaggag caggaagtat tgctctaggt
    32401 tatattccag tgatttagtt ggcacttcta ggctagttaa tcttagaaat tgaacacgga
    32461 ctacctgcct cttgaaaaaa gatgcctgtc ttgcctttcg tggaaagtca gtgccattcc
    32521 ctatttttca ggggcagcta aggtcctttg tatcttgata attgaacacg cctggaaccc
    32581 tgtgtgaaaa agatagttct tccaagaagt agacacttct tgatcagaat tcctattttc
    32641 actttgtgtt cttacaggct ttgtatcttg gacaggagta gagatgagta ttcgagtaat
    32701 tattgccggt tttaagggaa agatgggcca ggctgcttgt cagatggtat tgactgatcc
    32761 agacttggac ttggtagcag ttttggatcc ttttgagtct gagtcagaat ggcagggtat
    32821 tcctgttttc aaggataagg ctgatttagc tggttttgaa gcggatgtct ggatagattt
    32881 tactactcca gctgttgcct acgaaaatac acgttttgct cttgaaaatg gctttgctcc
    32941 agtagttgga acgactggtt tcacgagtga agaaattgca gagctaaaag aattttctcg
    33001 tgcccaagac ttgggtggcc tgattgcccc taactttgcc ttgggtgctg tcttactcat
    33061 gcaatttgcg acgcaggctg ccaaatattt cccaaatgtg gagattattg agctccatca
    33121 tgacaagaaa aaggatgctc cgagtggaac agccattaaa acagctgagt tgatggcaga
    33181 ggttcgagag tcaattcagc aaggtgcagc agatgaggaa gagctgattg ctggtgctcg
    33241 tggtgctgac tttgatggta tgcgcatcca ctcagttcgt ttgccaggct tggtagctca
    33301 tcaggaagtc atctttggca atcagggaga agggttgacc ctccgtcatg actcctatga
    33361 tcgcatctcc ttcatgacag gagtcaattt gggaattaaa gaagttgtca agcgtcatga
    33421 gcttgtctat ggattagaac acttattatg agattaacgc aaatgccttc tgaatttcag
    33481 aaggctttac cagtattaga aaaaattaaa gaagcaggct ttgaggctta ttttgttggg
    33541 ggctctgttc gagatgccct tctccatagc cctatccacg atgtggatat tgcgacgtct
    33601 tcttatccag aagagaccaa gcagattttt ccgcgaacag ccgatatcgg aatcgagcat
    33661 ggaaccgtct tggtcttaga tggggatgag gagtatgagg taacaacctt tcggacagag
    33721 gatgtctatg tggactatcg cagacccagt gcggtttcct ttgtgcgctc gctagaagaa
    33781 gacctcaaac gccgtgattt cacagtcaac gcctttgcct tggatgagac aggagaaatc
    33841 gttgacttgt tccatggttt agaagatttg gaaaagcaag tcttgcgagc agttggagtg
    33901 gctagtgagc gtttcaacga agatgcttta cggattatgc gtggtttccg ttttcaggct
    33961 agtcttggtt ttgcacttga gccagaaaca tttaaagcta tgaagacctt gacgccgctt
    34021 ttggagaaaa tttctgtaga gcgtaccttc gtcgagtttg ataaactctt gctggctcca
    34081 ttttggagaa ggggcttggc ttccatgatt gagagtcaag cttatgacta tctccctgat
    34141 atggcatcta gccaggacaa gctcaacaga ctgtttgatt tggagactga ttttactttt
    34201 gaatcctctg aacaagcctg ggcggcttta ctatgggctt tggagattga aaatgcgcag
    34261 tcatttttga aatcttggaa gacctcacgc cagtttgcca agcaagttca ggatttgctg
    34321 attattttgg ctctgcgtga aaatggagaa ttgagcaagc gagattgtta tcgctttgat
    34381 atagatttgc ttttacaggc tgaaaatctt cgtcaagctc aaggaaaaga agtcaaccta
    34441 caagccatca cagaaaaata ccaaagcttg actatccatg acaagaaaga gattcagatt
    34501 aatggcgata ttttgatcaa ggaatatggc tatcagccag gcccagactt gggagagatt
    34561 ttaacagaga ttgagtttgc cattgtcgat ggagaattgg agaataatcg tgaagccatc
    34621 catgcttacc tgagggagaa aaaatgagtg attttatcgt tgaaaaacta agtaaatccg
    34681 ttggtgacaa gaccgttttt agggatattt cctttattat ccatgactta gacagaattg
    34741 gtttaatcgg tgtcaatggg actggcaaga ccaccctttt ggacgtcctt tctggtgttt
    34801 ctggatttga tggggatgtc agtccttttt cagctaaaaa tgattaccag attggttact
    34861 tgactcagga tcctgatttt gatgatagaa agacagtttt ggatacggtt ctatctagtg
    34921 aactcaagga aatccagctc attcgtgagt atgaattgat tatgctcgac tatagtgagg
    34981 acaagcaggc gcgtttggaa cgtgtcatgg cagagatgga ctctctccaa gcttgggaaa
    35041 tcgagagtca ggtcaagacc gttcttagca aattgggcat tcaagactta tctactcctg
    35101 ttggggaatt gtcaggtggt ctgagaagac gggtacagtt ggcacaagtc ttacttggca
    35161 accacgacct tttgcttttg gatgagccga ccaaccatct ggatattgcg attattgagt
    35221 ggctgaccct ctttttgaaa aattctaaga agaccgtcct ttttatcact cacgatcgtt
    35281 atttcttaga cgctttgtca acacggattt tcgagttgga gcgagcaggc ttgacagaat
    35341 atcagggcaa ttaccaggac tatgttcgcc ttaaggcaga acaggacgag cgcgatgcag
    35401 ctctacttca taaaaaagaa caactctaca aacaagaact ggcctggatg cgcagacaac
    35461 cgcaggcacg tgcgaccaag caacaagctc gtatcaatcg tttccatgat ctgaaaaagg
    35521 aagtttcagg cagtagtgct gagacagact tgactatgaa ctttgaaacc agtcggattg
    35581 ggaagaaagt catcgagttt caggatgttt cctttgccta tgaaaacaag cccattttgc
    35641 aagattttaa tctcttggta caggtcaaag atcgtatcgg aatcgttggg gacaacggtg
    35701 ttggaaaatc aaccttactc aacttaattg caggaagtct tgagtcaact aaaggacaag
    35761 ttgtgattgg ggaaactgtt cgcatcgcct atttctctca gcaaattgag ggcttggatg
    35821 aaagcaagag agttatcaat tacctgcagg aagtggcaga ggaggtcaag accagtggtg
    35881 gttctacgac ttcgattgca gagttgctgg agcagttcct tttcccacgt tcgacgcatg
    35941 gaaccttgat tgagaaattg tcagggggtg agaaaaaacg tctttatctc ctcaaactgc
    36001 ttttggaaaa accaaatgtt cttcttttag acgagccaac caatgaccta gatattgcaa
    36061 ctttgacagt cttggagaat ttcttgcaag gttttgcagg tcccgtttta acagtcagtc
    36121 acgaccgcta tttcttggat aaggtagcga ccaagattct cgcttttgag gatggcaaga
    36181 ttcgtccttt ctttggtcat tacaccgact atcttgatga aaaagctttt gaaacagata
    36241 tggccaatca agtgcaaaag gccgaaaagg aaaaagtggt caaggttcga gaagacaaga
    36301 aacgcatgac ctaccaagaa aagcaggagt gggcaagtat tgaaggtgat attgaaacct
    36361 tggaaaaacg tatcgctgct attgaagagg aaatgcaggc taacggctct gactttggta
    36421 agctggctac tctccaaaaa gaattggatg agaaaaatga agcactcctt gaaaaatacg
    36481 aacgctatga gtatctcagt gaatttgata gttaagacta ttcgaaaatc aaagtgcaaa
    36541 ctatgtcggc ttcacattgc cgactcaagt acagcctgtg accggcttcc taatttgcac
    36601 tttgattttt attgagtata aaagaataga aaaatcccgt ctcatgaaag gatttctcta
    36661 ttcttttttg gtttcttgat gaaagatatt ttgccaggtt tcgtggcgac gccagatact
    36721 ggtatggatg ataccatcta actcatagca gatgagtttg gttttttgac tgatggaagt
    36781 aatttgaatg tccttgatag cagaatttaa ctcttttttg gtcttataag cctctttatc
    36841 catctgctct ccatcttggc gaatatagac aaaatcatca gtcaagagtt cttctagttg
    36901 gtttccttgg tcaatcaatt gttcacgcat aagcaatttt ttatagacat tgtctaaaat
    36961 ttcatcctca gggataggtg ctggttcgat atggacatcg gtatcaaaga cgccaaaacg
    37021 ctcctccagc atagactcga cctgatccgc gatttcatgg ctttcaaaaa cagacaagtc
    37081 aggattcatc tctagtgtaa tatccaggta gatgttgcta ccgtaggtgc gacctctttg
    37141 cgatttgacc ttgctgattt tgggaatttc catgatagcc ttttggtagt cctcgagcag
    37201 gcggtcgtca aagccatctg aaagactaaa ggaagactcg atgaagatat cataggcagt
    37261 cttcaagata aagaaagtga tgatgatagc aaccagttta tccacaatcg gataattgaa
    37321 actactagct aggatggcaa tggcggttcc aagtgaggta acagcgtcag aaagattgtc
    37381 cttagcagct gccttcagcg ccttggagtt ggatttctta ctgaggcgag tattgtagag
    37441 atagaccaca aacataatcg ctgcagaaat gattcctaga gttgcaccaa gaggatcaat
    37501 gaccgtttct tcccgactga gaatcttttg aatggtatct cttagaacat cgaaaccgac
    37561 atagaacatg atgatagaag tgatcaagct tgccaaatct tcaatcttcc aatgaccaaa
    37621 acggtggtca cggtctgcag gctggcgcgc catccgaatc ccgattaaga gggccacatt
    37681 tccaatgatg tccgatacgt tattaaaacc atcggccacc aaactggatg aatgaaggag
    37741 atgaccagct gctaatttgg ctgcagacaa gatcaaatag gtcgaaatac tgataatggc
    37801 tccacgctca gctaacttga gatttgagat agattgcttc attcaacttc ctttctagtc
    37861 acatagcatt ctaccattat actggttttc aaggaaaaat tcaaataagg cagtttttac
    37921 tctttgaaaa ctccccaaaa tttggtataa tggtactact caagggagta gctggcagaa
    37981 acctgtgata gtgtcgtcat tccgaatttt atgctgaaaa gtatgctttc cggccctatc
    38041 ttaaacagcg agacttgtta tgattaacag gtctcttttt gtttgtcgtg aaaagatatg
    38101 acagaaagag agtctgtttt gcacacaatg tcaaggagga gacacatgtc aaaagaacaa
    38161 aaacgccaag cgttttatac tcagagccct gaagaggtct tgcaggctgt ggatgcgacc
    38221 gagcaaggtt tgtcatcaag tgaggcggaa aagcgccttg ccgaatttgg gcacaatgaa
    38281 ctcgaagaag gcgagaaacg atcaatcttg gtcaaattca tcgagcaatt taaggatttg
    38341 atgattatca tcctagttgc ggcagcaatc ttgtcagtcg tgacttctgg tggggaagat
    38401 atcgcagatg ccattatcat cctagctgtg gttatcatca acgctgcctt tggtgtttac
    38461 caagaaggaa aagctgaaga agctattgaa gccctcaaat ccatgtctag tccagttgcc
    38521 cgcgttcttc gtgatggaca catggcagag attgactcta aagaattggt accaggcgat
    38581 atcgttgccc ttgaagcagg tgacgtggta ccagcggacc tacgtttgct agaagccaac
    38641 tctcttaaaa ttgaagaagc agccttgacg ggtgaatctg taccagtcga aaaagacttg
    38701 tcagtcgagc ttgcgacaga tgctggtatt ggtgaccgtg tcaacatggc cttccaaaac
    38761 tcaaacgtaa cctatggtcg tgggatgggt gttgttgtca atacaggtat gtacactgaa
    38821 gttggtcata ttgctggtat gcttcaagat gcggatgaga ctgatacacc actcaaacaa
    38881 aacttgaaca acctttctaa ggtcttgacc tatgctatct tggtcattgc ccttgttact
    38941 tttgtagtgg gtgtcttcat tcaagggaaa aatccacttg gtgagttgtt gacttctgtt
    39001 gcccttgctg ttgcagccat tccagaagga cttcctgcta tcgttaccat cgttctttct
    39061 cttggtactc aagttttggc caaacgacat tccatcgttc gtaagttgcc agcagttgaa
    39121 acacttggtt caactgaaat catcgcttct gataagactg gtacgctgac tatgaacaag
    39181 atgacagtcg aaaaagtctt ctacgatgcg gttctacatg actcagctga tgatattgaa
    39241 ctaggtcttg aaatgccact acttcgttca gttgtcttgg ccaatgatac gaaaatcgat
    39301 gtggaaggta acttgattgg tgacccaacc gaaacagcct ttatccaata tgccttggac
    39361 aagggctatg atgtcaaagg tttcttagag aaatatcctc gtgtagctga attgccattt
    39421 gactctgacc gtaagctcat gtcaacagtt cacccattgc cagatggtcg tttccttgta
    39481 gcagtcaagg gtgcgccaga ccaactctta aaacgttgtc ttcttcgtga taaggctggg
    39541 gatattgctc cgattgatga gaaggttaca aatctcattc atacaaacaa ttctgaaatg
    39601 gctcatcaag ccttgcgtgt ccttgcaggt gcttataaga ttatcgatag tattccagaa
    39661 aatctcactt ctgaagagct tgaaaatgat ttaattttta ctggtttgat tgggatgatt
    39721 gatcctgaac gtcctgaagc tgctgaggct gttcgtgtgg ctaaggaagc tggaattcgt
    39781 ccaattatga ttacaggtga ccaccaagac accgcagaag ctattgccaa acgtttggga
    39841 atcattgacg caaacgatac agaagaccac gttttaactg gtgctgagct caacgaatta
    39901 tctgatgaag actttgaaaa agttgttggt caatactctg tttatgcccg tgtgtctcca
    39961 gagcacaagg ttcgtatcgt caaggcttgg caaaaacaag gtaaggtcgt tgccatgaca
    40021 ggtgacggtg tcaatgatgc cccagctctg aaaacagcag acatcggtat cggtatggga
    40081 atcactggta cagaggtttc taagggggct tctgatatga ttcttgcaga tgataacttt
    40141 gcgactatta tcgtcgcagt ggaagaagga cgtaaggtct tctcaaatat tcaaaagact
    40201 attcagtacc tactttctgc caatactgct gaagtattaa ccatcttcct atcaaccttg
    40261 tttggttggg acgtcttgca gccagttcat cttttgtgga ttaacttggt aacggatacc
    40321 ttcccagcaa ttgcactagg tgttgaacct gcggaacctg gtgtcatgaa tcataaacca
    40381 cgtggacgca aggcaagctt cttctcaggt ggtgttttga gttctatcat ttatcaaggt
    40441 gtactccaag cagctcttgt tatgagtgtt tatggccttg cgattgctta cccagttcat
    40501 gtgggtgaca atcatgctat tcatgcagat gccctaacga tggcctttgc aacccttggt
    40561 ttgattcagc tcttccatgc ctacaatgtc aagtctgtct accaatccat cttgacagtt
    40621 ggcccattca agtctaagac ctttaactgg tccatcttgg tatcctttat ccttctcatg
    40681 gcaacaatcg ttgtagaacc gcttgaagga atcttccacg taaccaaact agacttgtca
    40741 caatggggaa ttgttatggc tggaagcttc tcaatgatta tcatcgtcga aatcgttaag
    40801 tttattcaac gcaaactcgg ttttgacaag aatgcgattt aagggaggtt tcggatgtca
    40861 gcatatcaat taccgaccgt atggcaggat gaagctagta atcaaggagc ttttacgggg
    40921 ctaaacagac caacagtagg tgcccgtttc gaacaaaact tgccaaaagg agaacaagct
    40981 tttcagcttt attcactggg aacaccaaat ggtgtgaagg ttactatctt attggaagaa
    41041 ttactagaag ctggttttaa ggaagcggct tacgacttgt ataagattgc tatcatggat
    41101 ggggatcaat tcggatcaga ctttgtgaag ctcaatccaa attccaagat tccagcctta
    41161 ttggaccagt caggtactga agatgtaaga gtctttgagt ctgctcatat tcttctttac
    41221 cttgctgaga aatttggagc ctttttacca agtaatcctg tggaaaaggt agaagttttg
    41281 aattggctat tctggcaagc aggtgcagca ccttttctag gtgggggatt tggacatttc
    41341 ttcaattatg ctcctgaaaa attggaatat cctattaacc gttttacaat ggaagtgaaa
    41401 cgccagttgg atttattgga taaggaattg gctcagaaac cttatattgc aggcaatgac
    41461 tatacgattg cagatattgc tatctggtct tggtatggac agttagttca aggaaatctt
    41521 taccaaggtt ctgcaaaatt cttggatgcc tcaagttatc aaaatctagt aaaatgggca
    41581 gaaaaaattg ccaatcgtcc agctgttaag cgtggcttgg aagtaactta tacagaaatt
    41641 aaatagcaaa aaagtcatct tcggatggct ttttgctaca gttgagggaa agggtttgca
    41701 gttatcggct tttgtgctat aattgctata atatagtaaa aagcaagagg agtgtgagga
    41761 agtggaaaag aaaattgtga aggatatctt atttttatct caagtgtctc agccggcaag
    41821 tcaggaggac ctttatcttg ccagagattt gcaggataca ctcttagcaa atcatgatac
    41881 ctgtgttggt ctagctgcca atatgattgg ggtgcagaag cgcgtgatta tctttaatct
    41941 tggcttggtt cccgtggtca tgtttaaccc agtgcttctg tcctttgaag gatcctatga
    42001 ggcagaagaa ggctgtttgt ccttggtagg cgtgagatca actaagcgtt atgaaaccat
    42061 aagggttgcc tatcgtgaca gcaagtggca ggaacagacc attaccttga caggcttccc
    42121 agctcagatt tgccagcatg agctggatca cttggaagga cgaatcattt aggaggaaag
    42181 caaatgaaac gaatagtctt tgaacttatt tttatcgcaa cgacctggta tatcttttta
    42241 ccgcccctta acctgaccag ctgggaattt ctcttcttcc tctgtgggca tttgttagtt
    42301 gtggcaatct tatttggctt tggcaagggg ataaaccttg tcaaaacggt tcatgtgcgc
    42361 cacggtaagg cggaagctgc cttaaatctt gagggtttca aaatcaatcg gttagggaaa
    42421 attctgttag cttcgattgg aggaattctt ctcttggcag ctttggtttc cttggtaact
    42481 tccagcatgt ttcaggctaa aaattatgcc aatgtagtca cggttacgga aaaagacttt
    42541 actgaatttc ctaggagtga caccagtaag gttcctatcc tagatagaag tactgctgaa
    42601 aaaattggag accgctactt gggttcccta accgataagg tgtcgcaata cgtagcggca
    42661 gatacctata cccaattgac aattgatggg aaaccttatc gggtcacacc actagaatat
    42721 gcagacccta tcaaatggtt taacaatcaa gccaagggaa tcggtgagta tatcaaggtg
    42781 gacatggtaa ctggaaatgc ggatttggtg gacttgaaga caccaatcaa gtattcagac
    42841 tcggagtatt ttaaccgtga tgtcaaacgt cacctgcgct tgaagtaccc gaccaaaatc
    42901 tttaaaactc catcttttga ggtggacgat gagggcaatc ctttctatgt agcaacggtt
    42961 taccaaaagc aatttggact tgctgttcct cgtcctgctt cagtcattat cttggatgct
    43021 acaaatggag aaaccaagga atacagctta tcagatgttc cagaatgggt ggacaggatc
    43081 tatccagcag aggaaaccat tgagcaaatc aactacaacg gcaagtacaa ggacggtttc
    43141 ttgaatgcca tgatttccaa gaaaaacgtg acccagacta ccaagggcta taattacttg
    43201 tctatcggta atgacatcta tctctacaca ggtgtgacgt cggctaatgc ggatgagagt
    43261 aatcttggtt tcatccttga aaatatgcga acaggagaaa tcactaagta tagcttggct
    43321 tctgcgacag aagaatcagc ccgtgaatca gcagaaggtg ctgttcagga gaaatcctac
    43381 aaagcaacct tcccaatcct catcaacctc aatgacaagc ctctctacat catgggcttg
    43441 aaggacaatg ctggcttggt caaagagtac gccctggtag acgcagtcga gtaccaaaat
    43501 gttatcgttg ctactacagt ggaagagatg ctcagcaagt atgccaataa aaacgacctt
    43561 gaaattgaca atgcaacgac agaaagcatc aatggagtag tagcagacct caaatcagct
    43621 gttatcaagg gagacactgt ctacttcttt aaagttgatg gcaagatata caaggtcaag
    43681 gcttcagtat ccgatgacct tccttacctt gaaaatggta aaaccttcga aggtcaagta
    43741 ggaaaagaca attatctcaa gacctttaaa gtacaataat actcttcgaa aatctctagc
    43801 tctatctgca acctcaa
//
LOCUS       DAVKGU010000007        40220 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKGU010000007 DAVKGU010000000
VERSION     DAVKGU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40220
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.007"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            60..224
                     /locus_tag="VW077_000354"
     CDS             60..224
                     /locus_tag="VW077_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9454437.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LAAVWLGWPK"
     gene            303..1031
                     /gene="yaaA"
                     /locus_tag="VW077_000355"
     CDS             303..1031
                     /gene="yaaA"
                     /locus_tag="VW077_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9454438.1"
                     /translation="MKILIPTAKEMNTDLPSIEATPLKPESQAVLDALALYSASQLES
                     FYKVSAEKATEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1309..1857)
                     /locus_tag="VW077_000356"
     CDS             complement(1309..1857)
                     /locus_tag="VW077_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9454439.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            2178..2666
                     /locus_tag="VW077_000357"
     CDS             2178..2666
                     /locus_tag="VW077_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9454440.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            2663..3850
                     /locus_tag="VW077_000358"
     CDS             2663..3850
                     /locus_tag="VW077_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9454441.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAAVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSTKEDNHFKVTVDQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            3843..4208
                     /locus_tag="VW077_000359"
     CDS             3843..4208
                     /locus_tag="VW077_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9454442.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            4224..5570
                     /gene="asnS"
                     /locus_tag="VW077_000360"
     CDS             4224..5570
                     /gene="asnS"
                     /locus_tag="VW077_000360"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9454443.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            5723..6013
                     /locus_tag="VW077_000361"
     CDS             5723..6013
                     /locus_tag="VW077_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9454444.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            6025..6495
                     /gene="ssbA"
                     /locus_tag="VW077_000362"
     CDS             6025..6495
                     /gene="ssbA"
                     /locus_tag="VW077_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9454445.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LERRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            6527..6766
                     /locus_tag="VW077_000363"
     CDS             6527..6766
                     /locus_tag="VW077_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9454446.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            7061..8461
                     /locus_tag="VW077_000364"
     CDS             7061..8461
                     /locus_tag="VW077_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9454447.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            8463..8822
                     /locus_tag="VW077_000365"
     CDS             8463..8822
                     /locus_tag="VW077_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9454448.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            8876..9625
                     /locus_tag="VW077_000366"
     CDS             8876..9625
                     /locus_tag="VW077_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9454449.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            9615..9914
                     /locus_tag="VW077_000367"
     CDS             9615..9914
                     /locus_tag="VW077_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9454450.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            9985..10920
                     /locus_tag="VW077_000368"
     CDS             9985..10920
                     /locus_tag="VW077_000368"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9454451.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            11006..11518
                     /locus_tag="VW077_000369"
     CDS             11006..11518
                     /locus_tag="VW077_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9454452.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLRDSDF"
     gene            11555..12073
                     /locus_tag="VW077_000370"
     CDS             11555..12073
                     /locus_tag="VW077_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9454453.1"
                     /translation="MIRYLDQYEDVILCENKRHYLNFPTLESLDSLELDQEIFVREAS
                     PVYQALLEQSFETELRNQINAAILVEKTDFARIKMTLSNYFYKVKQQYPLTEKQQELY
                     DILGDVNPEYALKYMTAFLLKFLKKDQLMQKCRDIFVDSLVVLGYIVQNEDRKYELAI
                     DFDKERLTFYLA"
     gene            12174..12371
                     /locus_tag="VW077_000371"
     CDS             12174..12371
                     /locus_tag="VW077_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9454454.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(12414..13859)
                     /locus_tag="VW077_000372"
     CDS             complement(12414..13859)
                     /locus_tag="VW077_000372"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9454455.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMVIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            13965..15587
                     /locus_tag="VW077_000373"
     CDS             13965..15587
                     /locus_tag="VW077_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9454456.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            15790..17748
                     /locus_tag="VW077_000374"
     CDS             15790..17748
                     /locus_tag="VW077_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9454457.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            17920..19020
                     /locus_tag="VW077_000375"
     CDS             17920..19020
                     /locus_tag="VW077_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454458.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALDNLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENR
                     PVLKDITCSIFKGQKLLLLDHLDQENQRLCVC"
     gene            19056..19601
                     /locus_tag="VW077_000376"
     CDS             19056..19601
                     /locus_tag="VW077_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9454459.1"
                     /translation="MEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLV
                     TDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARTYLKDAEILI
                     FDESTANLDAYSEYAIISILYSALKEKTVVIIAHRLSTVKDVDCIFFLEERKITGSGT
                     HKELLENHERYARFVQEQMIE"
     gene            19759..20853
                     /locus_tag="VW077_000377"
     CDS             19759..20853
                     /locus_tag="VW077_000377"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9454460.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVFAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            20861..22027
                     /locus_tag="VW077_000378"
     CDS             20861..22027
                     /locus_tag="VW077_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9454461.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNESKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            22120..25218
                     /locus_tag="VW077_000379"
     CDS             22120..25218
                     /locus_tag="VW077_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9454462.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINRQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADTNLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKVLPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILADSHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            25266..25883
                     /locus_tag="VW077_000380"
     CDS             25266..25883
                     /locus_tag="VW077_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9454463.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            25894..27228
                     /locus_tag="VW077_000381"
     CDS             25894..27228
                     /locus_tag="VW077_000381"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9454464.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYPEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEVEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            27238..27702
                     /locus_tag="VW077_000382"
     CDS             27238..27702
                     /locus_tag="VW077_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9454465.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            27699..28193
                     /locus_tag="VW077_000383"
     CDS             27699..28193
                     /locus_tag="VW077_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9454466.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            28274..29929
                     /gene="mltG"
                     /locus_tag="VW077_000384"
     CDS             28274..29929
                     /gene="mltG"
                     /locus_tag="VW077_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9454467.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVSTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     ISDTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            30000..30482
                     /gene="greA"
                     /locus_tag="VW077_000385"
     CDS             30000..30482
                     /gene="greA"
                     /locus_tag="VW077_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9454468.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(30695..30772)
                     /locus_tag="VW077_000386"
                     /pseudo
     CDS             complement(30695..30772)
                     /locus_tag="VW077_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            30900..31298
                     /locus_tag="VW077_000387"
     CDS             30900..31298
                     /locus_tag="VW077_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9454469.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            31766..32719
                     /locus_tag="VW077_000388"
                     /pseudo
     CDS             31766..32719
                     /locus_tag="VW077_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155340.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            32794..32994
                     /locus_tag="VW077_000389"
     CDS             32794..32994
                     /locus_tag="VW077_000389"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9454470.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            33029..33745
                     /gene="atpB"
                     /locus_tag="VW077_000390"
     CDS             33029..33745
                     /gene="atpB"
                     /locus_tag="VW077_000390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9454471.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            33759..34253
                     /gene="atpF"
                     /locus_tag="VW077_000391"
     CDS             33759..34253
                     /gene="atpF"
                     /locus_tag="VW077_000391"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9454472.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            34253..34789
                     /locus_tag="VW077_000392"
     CDS             34253..34789
                     /locus_tag="VW077_000392"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9454473.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDMLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            34804..36309
                     /locus_tag="VW077_000393"
     CDS             34804..36309
                     /locus_tag="VW077_000393"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9454474.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            36325..37203
                     /locus_tag="VW077_000394"
     CDS             36325..37203
                     /locus_tag="VW077_000394"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9454475.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            37289..38695
                     /gene="atpD"
                     /locus_tag="VW077_000395"
     CDS             37289..38695
                     /gene="atpD"
                     /locus_tag="VW077_000395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9454476.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            38706..39125
                     /locus_tag="VW077_000396"
     CDS             38706..39125
                     /locus_tag="VW077_000396"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9454477.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(39170..40183)
                     /locus_tag="VW077_000397"
     CDS             complement(39170..40183)
                     /locus_tag="VW077_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9454478.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT        12115 a         7488 c         8962 g        11655 t
ORIGIN      
        1 gtttatttaa taaatatatg ttataataag gtaaattaag cctaatggag gacaaagaaa
       61 tgggttttta tttgatggtt gcttcaatgc tactcggact gctcgctttg aagataggtt
      121 tttctcagtt caaggagaaa aaagataaat tcttatctat cttaacaagt ttagctggca
      181 tagcccttgt tctcgcggct gtctggttag gatggcccaa ataaatagaa aaacctcagt
      241 caaaccgagg tttttcaaat gaatttcttg gttgtggcta aaaaatatgc tacactatca
      301 atatgaaaat tttaatccca acagcaaaag aaatgaacac agacttgcca agtatcgagg
      361 caactccttt aaaaccagaa agtcaggccg tgcttgatgc cttggctctc tattctgcca
      421 gtcaattgga gagtttctac aaggtatcag ctgagaaagc gacggaagaa tttcaaaata
      481 tccaagcttt gaaaaggcaa actgctcaac actatccagc cttgaaactt tttgatgggc
      541 ttatgtaccg caacattaag agagataagc tgaccgaggc ggaacaagat tatcttgaaa
      601 atcatgtctt cattacctcg gctttgtacg gtgttgttcc agccttgtca cccatggctc
      661 ctcaccgttt ggattttttg atgaaattaa aagtcgctgg taagactttg aagagccatt
      721 ggaaggcagt ctatgatgaa gctctgaaga aggaagaagt gattttctct ctcttgtcat
      781 cagagtttga gactgtattt tctaaggaaa tcagagcaaa gatggtgacc ttcaaattca
      841 tggaggatag aggcggtcag ctgaagattc actcaactat ctccaagaaa gcgcgcgggg
      901 cctttctaac agctttaata gaaaatcaag tacaaactgt gggggaagca cgtcgcttga
      961 actttgctgg atttgtttac cgagaagatt tgtcacaacc acagggattg gtttttgtaa
     1021 aggaagtata gaaatcagat attttgtatt tggcaatatg tatcatcagc tgttttagtt
     1081 tataaatgag aaaaaaagcg aacaagctag tcttctgagt gtcagaaaag tagtcttgtt
     1141 cgcttttatt atgttatact cttcgaaaat ctcttcaagc cacgtcagcg tcgccttacc
     1201 gtatatatgt cactgacttc gtcagttcta tctacaacct caaagcagtg ttttgagcag
     1261 cttgcggcta gcttcctagt ttgctttttg atttttattg agtgttagtt acttgatagc
     1321 ttcgaccaag tcttgagctt gtttttcaag tgagtttagg actgtttctt caagaaccaa
     1381 ttttccgtct gcccaggcag agtcattaac acgtgcagca gtgaaatcac caacgccttg
     1441 tgtacggata aatggcaaga ggtctttgta gatagcgaaa agttgatcgt gccctgcatt
     1501 ggctacagat gatactgtga caaacttgtc ttgaagggca gaaacgccac gtgtatcaga
     1561 caagtcaagg gcacgagata gccagtcaag caagtttttc actgtaccag ggatagagaa
     1621 gttgtagact ggagagaaaa tccagatagc atccgcaacg agaactgctt cacgagcagc
     1681 agctacagct ggatgtgttg gaacttccaa atcttggctg aagagaggaa gggctgaata
     1741 atcaaggtag ctaacttccg ctttaccagc aagtgctttc tcagcttcga gcgccatttg
     1801 gtggttgaaa gaaccttgac gtagtgatcc gacgataaat aatacttttt tagacatgat
     1861 gtgtctcctt tttcttaaaa tttcgagaac tctctcgttg ttataaaata tattacaaca
     1921 aaacaaaaga acttgcaaga aatttgctca gagtttattt gaaaattgta aaatttcgga
     1981 aactctagtc atttgtgact gaaaaagtaa gaactgtttc actttcaaaa tcggttttgt
     2041 ttgaagggag aaatagaatt ggttgctcaa agcacagctt tgaggttgca gataaagctg
     2101 acgtggtttg aagagatttt cgaagagtat tcttatattc acttgtaata aagcttaata
     2161 gatggtaaaa tagacgagtg aaaaaaagac aaaacaaagc aaaaaataat ctactgtggc
     2221 agtatggtct agggatgacg attttgtttg tggttatcag tgcttccttt ctgtatatgg
     2281 tttctcttag catgaaaccc tatcaaacag ctaaaagtga aggagaaaaa ttagctcagc
     2341 agtatgcagg attagagcag gccgatcagg ttgatttata caatggcttg gaatcttatt
     2401 acagcgttct tggtcgtaat aaacagcaag aagcacttgc tgttctgatt ggaaaagatg
     2461 atcataagat ttacgtttat cagctaaatc agggtgtttc acaagaaaaa gcagaaacgg
     2521 tttctaagga aaagggagct ggcgaaattg acaagattat ctttggtcgt tatcaagata
     2581 agccaatctg ggaagtcaag tcaggatctg atttttatct agtagatttt gaaacaggag
     2641 cattggtcaa caaggagggc ctatgaaatt atccaaccgt gttttagaaa tggaagaaag
     2701 tgtgactttg gctgctggag cccgtgccaa agcactgaag gctgagggca gagatatttt
     2761 gtctctaacc ttgggtgagc cagatttcac tactcccaaa aatatccaag atgccgccat
     2821 tgcatcgatt cgagatgggc gtgcttcttt ttatacagta acctcaggtc tgccagagct
     2881 taaggcggcg gtcaatagct actttgagcg cttttacggc tattctgtag cgtcaaatca
     2941 agtgacagtc gctgcgggag ccaaatattc tctctatacc ttctttatgg ctgcggtcaa
     3001 tccaggtgat gaagtcatca tcccaacccc atactgggtt agctatggag atcaggtcaa
     3061 gatggcagaa ggcgttcccg tctttgtttc tactaaggaa gacaatcact ttaaggtgac
     3121 cgtagatcag ttagaagcag ctcgcactga caagaccaag gttttggtgc tgaattcgcc
     3181 atctaatccc acaggtatga tttacacccg tgaggaactc ttggcaattg gaaactgggc
     3241 tgtagaaaat gatattctca tcctagcaga tgatatctat ggccgcttgg tttataatgg
     3301 tcatgagttc acaccgattt ctagcctatc ggaagcgatt cgcaagcaaa cagtggtcat
     3361 caatggtgtt tctaaaacct atgccatgac tggttggcgg attggttatg ccgttggaga
     3421 agcagacatt atcgctgcca tgtccaagat tgcaggtcaa acaacttcga atccgtcagc
     3481 agtagcccaa tatgcagcag ttgaggctct atcaggtgag caagatactg tagaaagcat
     3541 gcgtcaggcc tttgaggaac gtcttaatac catctatccc ctccttgcag aggtgccagg
     3601 atttgaagtg gtcaaaccgc aaggggcctt ctatctcttc ccaaatgtca aaaaggccat
     3661 ggagatgaaa ggctacacgg atgtgacaga ctttacaact gctatcttag aagaagccga
     3721 agtggccttg gtaacaggag ctggatttgg agcaccagaa aatgtccgcc tcagctatgc
     3781 gacagaccta gacacgctta aagaagcagt cgaacgcttg aaagcattta tgggtagtga
     3841 gaatgattga tcattttgag attaaggtaa aggatttaca aatttcagaa ggattttata
     3901 ggagttttct cgctcctttg gactataaat tggcttttaa gactagttct ctaattagtt
     3961 ttctatcccc gaacagccct catcctggtg gtgatttttg gctgactcag ggaacacaag
     4021 atcctgttca ttttgctttt ttagcagaaa ataaggaaga agttcaggct tgttatgagg
     4081 ctggcttaga ggcaggtggg cgagacaatg gggctcctgg ttatcgaagt gagcatccga
     4141 tttactatgc tgcttttatg attgacctgg atgggaacaa tatagaggtg gtttgccata
     4201 aagaataaaa aagaaaagga aaaataatga caaaacgtgt aacgattatt gacgtaaaag
     4261 actatgttgg tcaggaagtg acgattggcg cttgggttgc caacaaatca ggaaaaggaa
     4321 aaatcgcttt cttacaattg cgtgatggaa cagccttctt tcaaggtgtg gcttttaaac
     4381 caaactttgt cgaaaaattt ggtgaagaag tgggacttga gaagtttgat gttatcaaac
     4441 gcttgagcca agaaacgtct gtttatgtga caggtattgt caaagaggac gaacgttcta
     4501 aatttggcta tgagttggac atcacagaca tcgaagtgat cggtgaatct caagactacc
     4561 caatcacacc aaaagaacac ggaacagact ttttgatgga taaccgtcac ttgtggctac
     4621 gctctcgtaa gcaagtagct gtgttgcaaa tccgtaacgc tattatctat gcaacttatg
     4681 agttctttga caagaacggt tttatgaagt ttgacagccc aattctttca ggaaatgcgg
     4741 cagaagattc tacagaactc tttgaaactg actacttcgg aacgccagcc tacttgagcc
     4801 aatcaggtca gctttaccta gaagcagggg ctatggctct tggtcgtgtc tttgactttg
     4861 gtccagtttt ccgtgctgaa aaatcaaaaa cacgccgtca cttgactgaa ttctggatga
     4921 tggatgctga gtactcatac ttgacacatg atgagtcgct tgacttgcaa gaagcttatg
     4981 tgaaagctct tctacaaggt gttcttgacc gcgcgcctca agccttggaa accttggaac
     5041 gtgatacaga actcttgaaa cgctacattg cagagccatt caaacgtatc acttacgatc
     5101 aagccattga cctcttgcaa gagcatgaaa atgatgaaga tgctgactac gagcatcttg
     5161 agcatggtga tgactttggg tcaccacacg aaacttggat ttcaaaccac tttggtgtgc
     5221 caacatttgt catgaactat ccagcagcca tcaaggcctt ctacatgaaa ccagttcctg
     5281 gaaatccaga gcgcgtgctt tgtgcagact tgcttgctcc agaaggctat ggagaaatta
     5341 tcggtgggtc tatgcgtgag gaagattacg atgcccttgt cgctaagatg gatgaacttg
     5401 gcatggatcg tacagaatat gaattctacc ttgaccttcg taaatacggt acagttccac
     5461 acggaggatt tggtatcggt atcgaacgta tggtaacctt cgcagcagga acaaaacata
     5521 tccgtgaagc tattccattc ccacgtatgt tgcaccgtat caaaccataa gaacaggaaa
     5581 acgcccatgt ggcgtttttt cgaattctag tgtttttact tgcgtaaaaa aaatttttct
     5641 agtataatag tttattgtga gcgaacctca cttacccctt gcaaagtctt ggggtcatta
     5701 gaccaaaagg aggaacatat caatggctaa atacgaaatt ctttatatca ttcgtccaaa
     5761 cattgaagaa gaagctaaaa acgctttggt agcacgtttt gactctattt tgactgacaa
     5821 cggtgcaact gttgttgaat caaaaacttg ggaaaaacgt cgtcttgcat acgaaatcca
     5881 agatttccgt gaaggacttt accacatcgt taacgttgaa gcaaatgacg atgcagctct
     5941 taaagagttt gaccgtcttt caaaaatcaa cgctgacatt cttcgtcaca tgatcgtcaa
     6001 aattgacgcg taagaaggta aactatgatt aacaatgttg tacttgtagg gcgtatgaca
     6061 cgtgacgctg agttgcgtta taccccatca aatgtagcag ttgcgacttt tactcttgca
     6121 gtaaaccgta catttaagag tcaaaatggt gaacgtgagg ctgattttat caatgtcgtt
     6181 atgtggcgcc aacaggctga aaatcttgct aactgggcta aaaaaggctc acttatcggg
     6241 gtgacaggtc gtatccagac tcgtagttac gataaccagc aaggacaacg tgtctacgtg
     6301 acagaggtcg tggctgagaa tttccaaatg ttggaaagac gtagtgtgcg tgagggtcac
     6361 acaggtggag cttactctgc accaactgca aactattcag cacctacaaa ttcagtacca
     6421 gacttttcac gtaatgaaaa tccatttgga gcaacaaacc cattggatat ttcagatgat
     6481 gatttaccat tctaatggac aactaaactt ataaaggaga aaaaacatgg ctcaacaacg
     6541 tcgtggcgga ttcaaacgcc gtaaaaaagt tgattacatc gcagcaaaca aaattgaata
     6601 tgttgattac aaagatactg agcttcttag ccgtttcgtt tcagaacgtg ggaaaatcct
     6661 tcctcgtcgt gtaacaggaa cttcagctaa aaaccaacgt aaagtaacaa cagctatcaa
     6721 acgcgctcgc gtaatggctt tgatgccttt cgtaaacgaa gattaaagaa aagacccagt
     6781 tgggtctctt tttcatgagc ttgaaaacaa gaaagcgaat taagacccca acgggtcttt
     6841 tttcacaagc tttgaaatga gagagcgagt taggctcagt ggctcccttt ttacgagttt
     6901 gaaaacaaga gaacacatta aaagcacgat ggagcttttt cataaatatg tctcttttct
     6961 catccaagaa attaaaataa aaatgataaa ctttttgaaa ataggggatt tgcctacttt
     7021 atgatataat ggaaagagtt agattgaaag aggtagtgag atggacgcga aattaagata
     7081 caaggcaaag aagatcaaga ttgtcttttt tgatattgat gatacattgc ggaattcaaa
     7141 gacagggttt attccaacta caattcccac tgtttttaaa cagttgcgtg aaaaaggaat
     7201 tttaacagga atcgcctctg gacgtggcat ttttggtgtt gttccagaga ttcgtgatct
     7261 caagcctgac ttttttgtaa ctttgaatgg ggcttatatc gaagataaaa aaggtcaggt
     7321 catttatcag catcagattg aaaagtcaga tgttgaggag tatatctctt gggctaagca
     7381 agaaggaatt gagtatggct tggttgggag tcatgatgcc aagttgtcga ctcgcaccga
     7441 tatgatgagt gaagctatca atccaattta tcccgactta gatgtagatc ccgatttcca
     7501 tgaaaaagaa gatatctatc agatgtggac ttttgaagat aagggagatg acttgcactt
     7561 gcctgacagt ctctcagaca aacttcgcat ggttcgttgg catcaacatt cgtctgatat
     7621 tgtgccgatt tcaggctcca aagcgacggg ggtggaaaag gttgtggaac accttggctt
     7681 gaaaccagag aaggtcatgg tttttggaga tggtctcaat gacttggaac tctttgatta
     7741 tgctggaatc agcgttgcca tgggaatttc tcatgataaa atcaaagaaa aagcagatta
     7801 tattacaaaa acattagaag aagatggcat ttttgatgcc ttagaagtat ttggtatggt
     7861 agaaaaagaa ttgcattttc cacaagtaga cattgaaaca gtagaaggtc ctcttgcgac
     7921 tattaagacc aatcacggag acttacgtat caagcttttc cctgaacatg ctcctaaaac
     7981 agtggctaac tttgtatctc tttcaaaaga tggctactat gatggtgtca ttttccaccg
     8041 tattatcaag gactttatga tccaaggtgg agacccaact ggaactggta tgggtggcga
     8101 gtcaatctac ggcgaatctt ttgaggatga attctcagaa gagctttaca atatccgtgg
     8161 tgctctttcc atggcaaatg ctggtccaaa taccaacggc agccagttct ttatcgtgca
     8221 aaaccaacac ctaccttatt ctaagaaaga aattactcgt ggtggttggc cagaaccgat
     8281 tgcagaaatc tatgccaatc aaggtgggac acctcaccta gaccgccgtc acacggtttt
     8341 tggtcagtta gctgatgaag catcttacgc tgtcttggat gtcattgctg ctgttgagac
     8401 aggagctatg gacaagccag ttgaagatgt tgtaattgaa actattgaaa tcgaggacta
     8461 agatgaaaat cggtgataag ctaaaggggc gtattacagg gattcagccc tacggtgcct
     8521 ttgttgagtt agagacgggt gatacggggc tgattcacat ttcagagatt cggacaggat
     8581 ttattgaaaa tattcatgaa accttgaaag tcgatgaaga ggttcaagtt caggtagtgg
     8641 atttagatga atttacaggg aaagccagtc tttctatccg cactttggag gaagaaaagt
     8701 accagtttcc tagacggaga cgcttttcaa gcgaccgctt taactacggt tttgctccct
     8761 ttcgacgaat gctaccaatc tggactggag aagccctgca ccatttaaaa aagaagaagt
     8821 agtttagagt tttatatttt ttgtaatgtt aatataaatt tgctataata ggaccatgga
     8881 agaagaacaa ttattaaaat caggagagcg cattaaccag ctcttttcga cagatatcaa
     8941 aatcattcaa aatagagagg tttttagcta ttcggtggat agtgttctct tatcacgatt
     9001 tccacgtttt cctaagaagg ggttgattgt ggatttctgt gctgggaatg gagcagtggg
     9061 gctttttgct agcactcgta ctcaagcaca gatattgtct gttgagattc aggagcgttt
     9121 ggcggatatg gctgaacgct ctgtccgttt gaatggatta gaggagcaaa tgcaggtcat
     9181 ctgcgatgat ttgaaaaata tgcctgctca cattcagggg agtaaggtgg atatgatttt
     9241 gtgtaatcca ccctatttca aggtggatcc ttattctaat ctgaacgaga gtgaacatta
     9301 tctcttggct cgacacgaaa tcacgactaa tttggaagaa atctgtcata gtgcccagag
     9361 tattctcaag tctaatgggc gtttggccat ggttcatcgt cctgatcgac ttctggatat
     9421 tttggatacg ttaaaacgac ataatctagc ccctaagcgc ctgcagtttg tttatccaaa
     9481 aagagaaaag gaagccaata tgcttttgat tgaggcgatc aaggatggct caacaagtgg
     9541 ctttaaggtc ttacctcctt tgattgtcca caatgatgat ggctcttata cgcccgaaat
     9601 cgaagagatt tattatggat cataaggctt atatgtacgt tctggagtgt cgtgatggat
     9661 cctactatat aggctatacg actgatatga gaagacgcct cgctatccac aatagtggga
     9721 agggagccaa atatacacga gcacgcttgc cagtcaaact tatctatgct caaggttttg
     9781 ccagtaagga agaagccatg tcggctgaag ctcttctcaa gcgtaagaag aggccacaga
     9841 aggaagaatt tttatctgaa aatcaagata gaaatttact cagttatttt gaagaatcgt
     9901 ggggagtcct ttgactcctc ttacctttgt caaaaagcga aaaaaatgct acaataaaga
     9961 aaataaacaa aatgaggtat tatcatgtcc aagattctag tatttggtca ccaaaatcca
    10021 gactcagatg ccatcggatc atctgtagct tttgcctacc ttgcaaaaga agcttacggt
    10081 ttggatacgg aagctgttgc ccttggaact ccaaatgaag aaacagcctt tgtcttgaac
    10141 tattttggtg tggaagcacc acgtgttatc acttctgcca aagcagaggg ggcagagcaa
    10201 gttatcttga ctgaccacaa tgaattccaa caatctgtat cagatatcgc tgaagtagaa
    10261 gtttacggtg ttgtagacca ccaccgtgtg gctaactttg aaactgcaag cccactttac
    10321 atgcgtttgg agccagttgg atcagcgtct tcaatcgttt accgtatgtt caaagaacat
    10381 ggtgtagctg tgcctaaaga gattgcaggt ttgatgcttt caggtttgat ttcagatacc
    10441 cttcttttga aatcaccaac aacacaccca acagataaaa tcattgctcc tgaattggct
    10501 gaattggctg gtgtgaactt ggaagaatat ggtttggcaa tgttgaaagc tggtaccaac
    10561 ttggctagca aatctgctga agaattgatt gacatcgatg ctaagacttt tgaactcaac
    10621 ggaaataatg tccgtgttgc ccaagtgaac acagttgaca tcgctgaagt tttggaacgc
    10681 caagcagaaa ttgaagctgc aatgcaagct gccaacgaat caaacggcta ctctgacttt
    10741 gtcttgatga ttacagatat cgtcaactca aactcagaaa tcttggctct tggtgccaat
    10801 atggacaagg tcgaagcggc tttcaacttc aaacttgaaa acaatcatgc cttccttgct
    10861 ggtgccgttt cacgtaagaa acaagtggta cctcaattga ctgaaagctt taatgcgtaa
    10921 gattttgggt gtcagctcaa aatcggaaag tctagtttgc cttatatcga aaggagtttc
    10981 ggctcctttt ttctaggagt gaagtatgtt agaaaatggc gatttgattt ttgtgagaga
    11041 tgggtcagac atgggacagg ccatccagac ttccacaggt aactatagcc atgttgccat
    11101 ttatttggat gggatgattt atcatgctag tggacaggct ggtgttgtct gtcaagaacc
    11161 ggcagacttc tttgagtcca atcatttata cgacctctat gtttacccag aaatggatat
    11221 ccagtcggtg aaggaaagag cttgcaaaca tcttggagca ccctacaatg cttctttcta
    11281 tccagatgca gctggtttct actgctccca gtatatagca gaaatcctac ctatttttga
    11341 aactattcct atgaaatttg gagatgggga acaggagatt agtgattttt ggagggagta
    11401 ttacatagaa ctaggtctgc ctgttcctct gaaccaagct gggaccaatc ctagtcagtt
    11461 ggcagcatcg cctctgttac aatgtaaaga aaggaatctt cgtgattcag atttttaatc
    11521 catctcgttt gacgagacag ccatttttgg agaattgatc cgctatctgg accagtatga
    11581 ggatgtgatt ctatgtgaaa ataagcgcca ttaccttaat tttcctacgc ttgaatcact
    11641 tgatagtctt gaactggatc aagagatttt tgtcagagaa gctagtccgg tctatcaagc
    11701 cttgttggag cagagttttg agacggaatt gcgcaatcaa atcaatgcag ctattttagt
    11761 tgaaaagacg gactttgcgc gcattaaaat gaccctgtcc aattattttt acaaggtcaa
    11821 acagcagtat cctttgacag aaaaacagca ggagctctat gacattttag gagatgttaa
    11881 tcctgagtat gccctcaagt atatgacggc ttttttgttg aaatttctca aaaaagacca
    11941 gcttatgcag aaatgccgtg atatctttgt ggacagtttg gttgtcttag gctatattgt
    12001 gcaaaatgaa gatagaaagt atgagttggc tatcgatttt gataaggaga gattaacttt
    12061 ctacttagcg tgatttcttg tttctgagaa cattgtttga ctttccttag tattcggtat
    12121 aaactatatg taaccggtaa cacatatcgg aataaactaa aggagacaat catatgtcac
    12181 ttgaaaacaa attggaacaa gcaacaggtg ctgtcaaaga aggttttggt aaagttactg
    12241 gagacagcaa gacagaactt gaaggagctg ttgaaaaaac agttgctaag gcaaaagacg
    12301 ttgtagaaga cgcaaaaggt gctgtagaag gtgccgttga aggtttgaaa aacgttttta
    12361 ctaaagaata ggaaaaaatc aagggtttca ttttcccttg attttttcta ttcttataaa
    12421 taattttctg cgacggctgt atctcctggg taggattctt tcttgccctg gatgatttgg
    12481 taacaatcgg ctcccttacc cgcaataata actgcatcta attcgtgatt tgtgatagcc
    12541 attgccgcct tgatggcttc ttggcgatcc gcaatctttt caacaggatg attgatgtag
    12601 ctactaattt catctgcaat gaccattggg tcttcatagt tagggtcatc agcagtcaga
    12661 aagacttgaa tctcagggtg ttgattgagg aggaggccaa agtccttacg acgactttct
    12721 cccttgtttc ctgttgatcc cagaaccaga gcaatctttc cggtttgatg agtttcaacc
    12781 acattgatga gttttttcag actatcccca ttgtgggcat agtcgatgaa gaccttggct
    12841 ccatttttct gagtgaggac ttccatacga ccaggaacgc gggttgcagc gatgcctttt
    12901 ttgatgtcct caagacttgc tccgagacgg agacaagcaa gtccagcagc aactgcattt
    12961 tcttggttga agttgccaat gagttgaata tcataatctc cagcgagttt acccgtagct
    13021 gaaaagctaa aggctttgga attctcgatt tggttatcaa attggctacc atagaaatca
    13081 tggtcttgat cttcaacctg ttctttcaag actgagaagt ggtccatgtc actgttaatg
    13141 atgactgctc ggctattttc catcaagaga cgcttgtggt agaaatagtc ttcaaagcta
    13201 gggtgttcaa tcgggccgat atggtctggg ctgatattta ggaaaactcc cacatcaaag
    13261 gttagaccat agacacgttt gaccagatag gcttgactgg agacttccat gatgaggtgg
    13321 gtacggtcat tttgcacagc ctgattcatc atgtcaaaga ggtcaatact ctcaggggtt
    13381 gtcaacgctg acttaaagaa agtctcgcca tcaagagttg tgttcatggt cgacaacata
    13441 gcaggtctat gcccttgaga taagatgtta taggcgaaat aggctgctgt tgtcttaccc
    13501 ttagtaccag taaaggcaag gagtttgagt ttttcctgtg gattaccata gaactccatg
    13561 gcaatcaaac tcatggcttt ctttatatcg ttcacaatga tgacagggat accgacttcg
    13621 tagtcctttt cagctacata ccaagctaat ccttgtgtta tagcagaaag aaggtattct
    13681 tttttaaagg cagcgccttt tgcgaaaaaa agagtgtctt ctgttacttt tcggctgtcg
    13741 tagctgatgc tatcaaaaat aactttgctg tagttgtagt ggtaatgacc ttggtcaata
    13801 atttcgcgaa aaaggccatc tttctttaaa atatctaata cggtttcaat cttaatcata
    13861 ctttctattg taaaccgaaa gtcgtaaatt tacaagtaac aaggaaaagt ttataatgga
    13921 agataaggag tttttcctag ctatcaaaat tgaatgagga atctatgtcg cacgaaaaca
    13981 atcaccagca ggcccagatg ttacggggga ctgcttggct aacggctagt aactttatca
    14041 gtcgcctact cggggctgtt tacattatcc cttggtacat ctggatgggg gcttatgcag
    14101 ctaaggcaaa tggtctcttt accatgggtt acaatatcta tgcttggttc ttgttggttt
    14161 caacagcggg gattccagtt gcggtggcca agcaagttgc caagtataat accatgcgag
    14221 aagaagagca tagctttgcc ctgattcgga gcttcttagg ctttatgaca ggactaggcc
    14281 tggtttttgc tttagtcttg tatgtctttg ctccttggct agcagacttg tctggcgtgg
    14341 gcaaagactt gatcccaatc atgcaaagct tggcttgggg agtcttgatt ttcccgtcta
    14401 tgagtgttat ccgaggattt ttccaaggga tgaataacct caaaccctat gccatgagcc
    14461 aaattgctga gcaggtcatt cgtgttatct ggatgctcct agcaaccttt atcattatga
    14521 agctcggttc aggagattat ctagcagccg ttacccaatc aacctttgct gcctttgtcg
    14581 gtatggtagc cagttttgca gtcttgattt atttccttgc ccaagaaggt tcactcaaaa
    14641 gagtctttga aacaggagat aagattaaca gtaagcgtct cttggttgat accattaagg
    14701 aagccattcc ttttatcctg acagggtctg ccatccagct cttccagatt ttggatcagc
    14761 tgacctttat caatagtatg agctggttta ccaactacag caatgaggac ttggttgtca
    14821 tgttttctta tttctcagcc aatcctaata aaatcacgat gattttgatt tctgtagggg
    14881 tttcgattgg gagtgttggt ttgccacttt tgacggaaaa ctatgtcaag ggggacttga
    14941 aagcagcttc tcgtctggtt caggacagtc tcaccctact ctttatgttc ttgctaccag
    15001 caacggttgg agtggttatg gtaggagaac ctctatatac agtcttctat ggtaagccag
    15061 atagtttggc tctgggctta tttgtctttg cagttttgca gtctattatt ttaggcttgt
    15121 acatggtctt gtctccaatg cttcaggcca tgttccgcaa ccgaaaggcc gttctctatt
    15181 ttatctatgg ttctattgcc aagctagtct tgcaactacc taccatcgcc ctcttccaca
    15241 gttatggtcc tttgatttca acaaccattg ctctcatcat tcctaacgtc ttgatgtatc
    15301 gggatatttg taaagtaact ggtgtcaagc gcaaggtgat tttgaagcga accattttaa
    15361 tcagtttgct gaccctagtc atgtttctgt taataggaac catccagtgg ctgttaggat
    15421 ttttcttcca accaagtgga cgtttgtgga gcttctttta tgtagctctt gtcggtgcca
    15481 tggggggtgg actttatatg gttatgagtc tgcgtaccta tttattagat aaggtaatag
    15541 gaaaagccca agcagatcgc ctgcgagcaa aatttaagct ttcgtaaggt agcatttata
    15601 aataaaagga ataagttgaa caatctttta ggagaacttg aagcttattc cttttttagc
    15661 gtcaaaatag ctaaaattat ctttcaaaaa tagaaaatga ggtttttttg attttttgga
    15721 aaaaacaatt gactaaacaa accaagagtt gtataataaa acaaatattt aaagcaggag
    15781 gttctggaaa tgaaaaagtc taagagcaaa tatctaacct tggcaggtct tgtcctgggt
    15841 acaggagttt tattgagcgc gtgtggaaat tctagcactg catcaaaaac ctataactat
    15901 gtttattcaa gtgatccatc tagtttgaac tatctagcag aaaaccgcgc agcaacatcc
    15961 gatattgttg caaatttggt agacgggtta ttagaaaatg accaatatgg gaatattatt
    16021 ccatcattag cagaggattg gactgtttct caggacggtt tgacctatac ctacaaactt
    16081 cgtaaggatg ccaagtggtt tacttctgag ggagaagaat atgcgcctgt aactgcccag
    16141 gattttgtga caggtttgca atatgcagct gataaaaaat cagaagcctt gtatctagtg
    16201 caggactctg ttgctggttt ggatgactat atcactggta aaacaagcga cttttcaact
    16261 gtcggtgtca aggcacttga tgaccaaacg gttcaatata ctttggttaa accagaactt
    16321 tactggaatt caaaaacact tgcaacgata ctttttcctg ttaatgcaga tttcctgaaa
    16381 tcaaaagggg atgattttgg gaaggcggat ccatctagta ttttgtacaa tggacctttc
    16441 ttgatgaaag cacttgtctc aaaatctgct attgaatata agaaaaaccc taattactgg
    16501 gatgctaaga atgtctttgt agacgatgtg aaattgacct actatgatgg tagcgaccaa
    16561 gaatcactgg aacgtaattt tacagctggt gcttatacta cggctcgtct ttttcctaac
    16621 agctccagct atgaagggat taaagaaaaa tacaaaaaca atatcatcta tagtatgcaa
    16681 aattcaactt catatttctt taattttaac ctagatagga agtcttacaa ttatacttct
    16741 aaaacaagtg acattgaaaa gaaatcgact caggaagcag ttctcaataa aaacttccgt
    16801 caggctatca attttgcttt tgacagaaca tcttatgggg ctcagtctga agggaaagaa
    16861 ggtgcaacaa agattttgcg taacctagtg gttcctccaa actttgtcag tatcaaggga
    16921 aaagactttg gtgaagttgt agcctctaag atggtcaact atggtaagga atggcaaggt
    16981 atcaactttg cggatggtca agacccttac tacaatcctg agaaagccaa ggctaagttt
    17041 gcggaagcta agaaagaact cgaagcaaag ggtgttcaat tcccaatcca cttggataag
    17101 actgtggaag taacagataa agtaggcata caaggagtta gttctatcaa acaatcaatt
    17161 gaatctgttt taggttctga taatgtagtg attgacattc agcaattaac atcagatgag
    17221 tttgacagtt caggctactt tgctcaaaca gctgctcaga aagactatga tttatatcat
    17281 ggcggttggg gacctgatta tcaagacccg tcaacctatc tcgatatttt taatactaat
    17341 agtggaggag ttctacaaaa tcttggacta gagcctggag aagccaatga caaggctaag
    17401 gaagttggac tggatgtcta tactcaaatg ttggaagaag ctaataaaga gcaagatcca
    17461 gccaaacgtt atgagaaata tgctgatatt caagcttggt tgattgatag ttctttagtt
    17521 cttccaagtg tttcgcgtgg gggaacacca tcattgagaa gaaccgtacc atttgctgct
    17581 gcctatggtt taaccggtac aaaaggggtt gaatcatata aatacctcaa agtacaagat
    17641 aagattgtca caacagacga atatgcaaaa gccagagaaa aatggttgaa agaaaaagaa
    17701 gaatccaata aaaaagccca agaagaattg gcaaaacatg tcaaataaaa tctaattgta
    17761 gataagtttg tgtaagaagt ctttgtgctt aaaaacgcat aatatcaggt atataaaatc
    17821 ctagatgtta tgcgttttgt tataggaaga tttattagat tttatcctca aattgagtta
    17881 ttcccgactt tatccgttga aatattataa ggagtattta tgaaattaaa attattaaga
    17941 gtagatacta aggtgattat ggggagtttc ttccttgttc tgtctagtct acttgctttg
    18001 ttgcttcccc ttatcttaaa gggtttaata gatgggagtt ctattgaaaa tataggttcc
    18061 aaagtatttc aatcgttttt gatttttatt ggtcaagcct tgttttcttc tattggttac
    18121 tatctgttta gtcaatcggg tgaaaaaaag atagcaaaaa tcaggaaaaa agtgatagag
    18181 gggttgattt atgcagagaa atccttcttt gataagagcc aaagtgggga gttgacttct
    18241 gccattgtca atgacacgag tgtcattcgt gagtttttaa ttacgacttt cccaaatatt
    18301 attctgagtt tagttatggt acttggttcc attgtagtct tatttagtct tgattggaat
    18361 ctttctctac ttttattcat cactcttcct tgtatgatgt ttattatctt gcccctttcc
    18421 aatatcagtg aaaagtatag tcgtcgttta caggaggaaa tcggattttt aacagggcaa
    18481 ttgactgaaa agattcaaga acatgaatta attaagacta accaagcaga gaaaagtgta
    18541 caggatgttt tggataattg tattgaaagg gtacaaaaca attcactgaa atcagatagg
    18601 gttacttctt ttgagacccc gtttgctctc ttatttatct ttgcgactat agttgtgatg
    18661 ctaacctatg ggggttatcg ggtcagcgca ggatatatat ctgtgggaac cttggtttcg
    18721 tttttgattt acctctttca attacttaat cctattagta atatagctaa ttttgtaact
    18781 gtttattcta ggagcaaggg atcttcagtt gcactggata acttgcttgc agttcctaaa
    18841 gaaaaatttg agggaggaaa atcggtatca ggacaagggt tgaattttaa ccatgtctat
    18901 tttggttatg atgaaaatcg acctgtctta aaggatatta cttgttcaat tttcaagggg
    18961 caaaaattgc ttttgttgga ccatctggat caggaaaatc aacgattgtg cgtttgttag
    19021 agcggtttta taaaccgctt tcaggagata ttctaatgga gcaatcaagt atatatgatt
    19081 ttaacttaaa agaatggaga agtaaaatcg cttgggtttc acaaaataat gcagtcttat
    19141 ctggcagtat tcgtgacaat ctttgtctcg gtttgaatcg cttagtaact gatgatgaat
    19201 tgatgaaagt gctagactta gtatcactag gtgatgagat tcgctccatg aaagagggac
    19261 tagatactga agttggtgaa cgcggacgat tcttgtcagg ggggcaaagc caaagacttc
    19321 aaatagctag aacctactta aaagatgctg aaattcttat atttgatgaa tccactgcta
    19381 atcttgatgc gtattctgag tatgcgatta tcagtatcct ctattctgca ttaaaggaga
    19441 agacggttgt gattatagcg catcgtttgt caacggtaaa agatgtggat tgtattttct
    19501 tcttagagga gaggaaaatc actggctcag gaactcataa ggaactactg gaaaatcatg
    19561 agcgttatgc tcgttttgtg caggagcaaa tgatagagtg aaggtgtctt ttgagattca
    19621 ccattttata gtctattaaa gggagcagga aaaactccct ttttatatag tttgaaacta
    19681 taactagctc ttgaaaagaa gaaaatgagt tgatgaaaat aagtggtaca atagttacta
    19741 tagatttgga ggtattgtat gagcaaggaa ttacacatta acacaatttt ggcccaggcg
    19801 ggtattaagt cagatgaagc gacaggtgca ttggtgacac cgcttcattt ttcaacgacc
    19861 tatcagcatc cagagtttgg tcgatctact gggtttgact atacgcgcac taaaaatcca
    19921 actcgtagta aggctgagga agtctttgcg gctattgagt cagcagacta tgccctagcg
    19981 actagctcag ggatgtcagc tattgtactg gcctttagcg tctttccagt aggaagtaag
    20041 gtcttggcag tgcgtgatct ttacggtggt tcttttcgct ggtttaacca agtggagcag
    20101 gaaggtcgtt tccattttac ctatgccaat acagaagaag agttgattgc cgagttagaa
    20161 aaggatgtgg atgttctcta tatcgaaacc ccaaccaatc ccttgatgtt ggaatttgat
    20221 atcgaaaaac tagcaaaatt ggctcatgct aagggtgcca aagtggtggt ggacaatacc
    20281 ttctatagcc ctatctacca acgtccgatt gaagatggag cagatatcgt tctccattca
    20341 gcaaccaagt atctagcagg ccacaatgat gtcttggctg gagtggttgt gaccaatagt
    20401 ttagaactat acgagaagct tttttacaat cttaatacaa caggggcagt cttgtctcca
    20461 tttgacagct accagttgct tcgtggtctc aagaccttgt ctcttcgtat ggagcgctca
    20521 acagctaacg cccaagaagt ggttgccttt ttgaaggatt ctccagcagt taaggaagtt
    20581 ctctacactg gtcgtggagg catgatttcc tttaaagtag ccgatgaaac acgcattcct
    20641 catattttga acagtctcaa ggtcttctct tttgcggaaa gtttgggcgg agtggaaagt
    20701 cttattactt atccaacgac tcaaactcat gctgatattc cagcagaagt acgccattct
    20761 tatggtttga cagatgacct cttgcgtttg tctattggga ttgaggatgc tagagatttg
    20821 attgcagatt tgcgccaagc cttagaagga taagacaaag atgggaaaat atgattttac
    20881 aagcctgccc aaccgtttag ggcaccatac ctataaatgg aaagaaacag aaacggatag
    20941 tgaagttcta ccagcttgga tagcggatat ggactttgtg gtcttgcctg aaatccgcca
    21001 agccgtgcaa acttacgcag accaactggt ttatggttat acctatgcca gtgaagactt
    21061 aattaaggaa gttcaaaagt gggaagctac acaatacggt tacaactttg acaaagaggc
    21121 tcttgtcttt atcgagggtg tggtaccagc catctcaaca gctattcaaa cctttacaaa
    21181 agaaggcgag gcggttttaa ttaacacgcc tgtctaccca ccctttgctc gcagtgtcaa
    21241 gttgaataat cgtagattga ttactaattc cttagtggaa aaggatggtc tgtttgagat
    21301 tgactttgac caacttgaaa aggatttggt ggaagaggag gttaaactct atattctttg
    21361 caatcctcac aatcctggtg gacgtgtttg ggaaaaagaa gtgttggaga agattggcca
    21421 actctgccaa aaacacggtg ttttgttagt ttcggatgag attcaccaag atttgaccct
    21481 ctttggtcac aaacaccagt ctttcaatac catcaatcct gccttcaaaa attttgctat
    21541 cgtcttgagc agtgccacta aaacatttaa tattgctgga acaaaaaatt cctatgcagt
    21601 cattgaaaat cctaagttga gactggcttt ccagaaacgc ctgttggcca ataatcagca
    21661 tgaaatttca ggcttgggtt atttggcgac agaagctgcc tatcgatacg gtaaagattg
    21721 gctagaggaa ctcaagcaag tctttgaaga ccacatcaat tatgtggtag atctatttgg
    21781 aaaagagact aaaatcaagg tcatgaaacc gcaaggtacc tacttgattt ggcttgactt
    21841 ttcagcctat gacctgactg atgaaacatt gcaagagttg ttgagaaatg aatccaaggt
    21901 tatcctcaac cgtggtttgg attttggaga ggaaggaagt ctccatgccc gcatcaatat
    21961 agctatgccc aaatctctgt tgcaggaagt ctgtcagcgg attgtggcta cttttgccaa
    22021 acgttaaaaa tccagtcttc taggagaaaa gtcttcctag aaggctattt tcataggcga
    22081 aaatatggta taataaaaag ataaggtaaa ggtgaaaata tggctaaatt gattccgggg
    22141 aaagttcgta tcgaaggtgt tgccctttat gaaactggta aggttgatat catcaaggaa
    22201 aagaacaatc ggctctacgc tcgcgttgca gaagaagaac tgcgctatag tttagaggat
    22261 gatttggttt tttgtgcctg tgattctttt caaaagaggg gctactgtgt gcatttggca
    22321 gcgctagagc attttctgaa aaatgatgag cgtggtcagg aaatcttgtg gagtctggaa
    22381 gaaggtcatg aagaaaaaga ggccgttgaa accaaggtga ccttgggtgg caagtttttg
    22441 aatcgaattt tatctccgaa atcagaatgc gcctatgagt tatcagctgt gggtcaggtg
    22501 gaagcaggaa ccaatcatat cttgtggact ctgagaatcg gccaaatcaa tagacaaaaa
    22561 tactatgtca ttcgggatat tccacttttt ttaaggattg tcgagcagag aaagtcgtat
    22621 atgattggca agatttatga ggagtctctt tcttgggaag cctttgatga tgccagccaa
    22681 gaacttttga ctttcttgca cggactaacg gaggaagggc tggttccaga cctctttttc
    22741 caaaaccaag gtcggcacct ctttttccct ctgacctttt ttgagcaggg tgtgaattta
    22801 ctgatggcct tgccccattt tcaatttgac catcaagtgg atagctacca aaccctgctt
    22861 tttcaggata tgcatgctga taccaatcta tttgccttta cagtaacaga atactcggac
    22921 tattttgaaa tggaaatcag tgagagtcct agggtcaatg tcttttatca gggagcagta
    22981 cttttccata aaggtcaggt ttatttttta acagaccagc agatgcgtct tctcaaggaa
    23041 attaaagtgt tacctgtggg tcaacaagga aagaaatacc tgcaatttga tagcagtgat
    23101 cgagacaaat tggcctcctg tctgactctt tttatccaga tgggcaccgt ttcagctcca
    23161 gaacgtctac aaatcaaaac ttttgcgccc tctttttact ttgacaggga agaagataat
    23221 cggattcgtt tagaaattca gtttgattat ggaaatagac aggtatctag ccgacaagag
    23281 ctagaagaat taccattttc aagtgacgcg gacttagaag aaagaatttt ccaagtctgt
    23341 ttggctgcgg gctttgaagc cgattttcaa tcttggcgtc aggccttaaa agcggagtct
    23401 gtctatcatt tttttcatga tatcattcca gtctttgaaa aactcgggca tgttgaccta
    23461 tcagataaac tagaagagct ttatagccta gcgagtcctc aggtgcagat tgcctccaag
    23521 ggaggtctct tggaaatcca gtttgatttt caaaatattg cccaggagga aatcgaccaa
    23581 gccatgcagg ccttggttgc caatcaggat ttttatattg attcgtctaa tcaagtttac
    23641 tttttcgatg aagaaaccaa gaaaattcgc caaaatctac aggaactggg gcaatttgaa
    23701 ttaaaagatg ggaccttgca ggctcggaaa tccttggcct acagtttagc tcatctcttt
    23761 gaagggcgag accgtgtttc tttttcacaa gaattccaga atctggccca ggatttgacg
    23821 catccagagg actttccttt gcaagcaact caggtcaagg ctgacttgcg agattatcag
    23881 gaaaagggaa ttggctggtt gcagatgctc catcattatg gttttggtgg gattttggct
    23941 gatgatatgg gacttgggaa aacccttcag accattgctt ttttgactag tcaagtgaca
    24001 aaagaaagtc gagtcttgat tctcgctccg tcgggtttga tttacaactg ggcggatgag
    24061 tttcagaaat ttgccccaca gttggatgtg gctgttgttc atggtttgaa agcaagtcgt
    24121 gaggagattc ttgccgatag ccatcaaatc tatgtgacta gctatgccac cttccgtcag
    24181 gacagtgagc tttatcaggg gatggccttt gacttccttt tcttagatga agctcaggtc
    24241 atgaaaaatg cccagaccaa gattgcccag accttgagac aatttgtggt gccgtcggtc
    24301 tttgccttgt caggaactcc gattgaaaac catctgggtg agttgtggtc tattttccaa
    24361 atcgtcatgc ccggactttt gccaagcaag aaagaattta tgaaattgcc agcagagcga
    24421 gtggctcagt ttatcaagcc tttcgtgatg cgacgcaaga aagaagaagt tctgactgaa
    24481 ttgccagact tgattgaggt ggtttataaa aatgaactgg aagaccagca aaaggctatt
    24541 taccttgccc agttacaaca gatgcgagac catctggctc aagtgtcaga acaggaattt
    24601 cagcgaagtc gtgtggaaat tttatctggt ttgatgcgct tgcgtcaaat atgtgacact
    24661 cctgccctgt ttatggaaga ttatcaggga gccagcggca aactagatag tctccgagac
    24721 ctactggtac aggtggcaga cggcggacac cgtgtcttga ttttctctca gttcaaggga
    24781 atgttggaaa aaattgaaca agaactgcca gacttgggcc tgacttcctt taaaattacg
    24841 ggttcaaccc cagccaagga aagacaagac atgaccaagg cctttaacca aggagaaaga
    24901 gatgcctttc tgatttccct caaggctggt ggggtcggtc tgaacctgac aggtgcagat
    24961 acagtgatct tggttgacct ttggtggaat cctgcggtgg aagcgcaagc cattggccgt
    25021 gcccatcgga tgggtcagga agaaacggtt gaggtctatc gcttggtgac caagggaacc
    25081 attgaagaaa aaattcagga actccaagaa caaaagaaac atctggtgtc acaagtattg
    25141 gacggcacag agtcacgtgg tagtctgacc ctagcagaaa ttagagaaat tttgggaatt
    25201 tctgaagcca gcacttgaaa aatcaagaca atacgctata atgaagtgtt gaaaggaaat
    25261 agaaaatgaa acaagaacga tttccattgg tgtcagatga cgaggtcatg ttgactgaaa
    25321 tgccagtcat gaatctctat gatgagtctg atctgatcag taatatcaag ggtgagtatc
    25381 gagataaaaa ttatttagaa tgggctccta ttgctgaaga aaaaccagta aaaccgattg
    25441 aaaagcaagt cgaaaaacct aaaaaggctc ctttaggggt taaaaaagaa ggaaagagct
    25501 atgcggaggt ggcgcgtgaa gaagcgcgtg cggacttgaa aaagaaacgc tctgctaact
    25561 acctaactca ggatttcagc cttgcgagac gtcattctca gcccagtcta gttagacagg
    25621 gcaatcaacc gacaactcct ttccaaaagg aaaatcctgg tgaatttgtc aaatatagcc
    25681 aaaaattgac ccagtctcat tatatcttgg cggaagaagt tcattctatc cctaccaaga
    25741 atgaagaagt gtcagcacct gctccaaaga aaaacaatta tgattttcta aagaagagcc
    25801 aaatctacaa taaaaaaagt aaacaaacag aacaagaacg tcgggttgcc caagagttga
    25861 atctgaccag aatgacagaa taggggagaa aacatgtcaa agacatatca ttttatcgga
    25921 attaagggat cagggatgag tgccttggcc ttgatgttgc accagatggg gcacaaggtt
    25981 cagggatcag atgttgaaaa gtactacttt acccaacgcg gtcttgagca ggcaggaatt
    26041 accattcttc cttttgatga aaagaatcta gacggtgata tggaaattat cgctggaaat
    26101 gcctttcgtc cagataacaa cgtcgaaatt gcctatgcgg accaaaatgg tatcagctac
    26161 aaacgttacc ctgagtttct aggtagcttt atgcgtgact ttgttagcat gggagtagca
    26221 ggagcacatg gaaaaacttc aacgacaggt atgttgtctc atgtcttgtc tcacattaca
    26281 gataccagct tcttgattgg agatgggaca ggtcgtggtt cggccaatgc caaatatttt
    26341 gtctttgaat ctgacgaata tgagcgtcac ttcatgcctt accacccaga atactctatt
    26401 atcaccaaca ttgactttga ccatccagat tatttcacaa gtctcgagga tgtttttaat
    26461 gcctttaacg actatgccaa acaaatcacc aagggtcttt ttgtctatgg tgaagatgct
    26521 gaattgcgta agattacgtc tgatgcacca atttattatt atggttttga agttgaaggc
    26581 aatgactttg tagctagtga tcttcttcgt tcaataactg gttcaacctt caccgttcat
    26641 ttccgtggac aaaacttggg gcaattccac attccaacct ttggtcgtca caatatcatg
    26701 aatgcgacag ccgttattgg tcttctttac acagcaggat ttgatttgaa cttggtgcgt
    26761 gagcacttga aaacatttgc cggtgttaaa cgtcgtttca ctgagaaaat tgtcaatgat
    26821 acagtgatta tcgatgactt tgcccaccat ccaacagaaa ttattgcgac cttggatgcg
    26881 gctcgtcaga aatacccaag caaggaaatt gtagcagtct ttcaaccgca tacctttaca
    26941 agaaccattg ccttgttgga cgactttgcc catgctttaa accaagcaga tgctgtttat
    27001 ctagcgcaaa tttatggctc ggctcgtgaa gtagatcatg gtgacgttaa ggtagaagac
    27061 ctagccaaca aaatcaacaa aaaacaccaa gtgattactg ttgaaaatgt ttctccgctc
    27121 ctagaccatg acaatgctgt ttacgtcttt atgggagcag gagacatcca aacctatgaa
    27181 tactcatttg agcgtctctt gtctaacttg acaagcaatg ttcaatagga tgttcccatg
    27241 gaaattccaa ttaagatcat tcaggcaagc aagtctgatt tgcctgagat aggggcactt
    27301 cagacctcgt cttttccagc tgaaaagcag caactttccc atattttaga agaaagtatc
    27361 cgtaagtgtg cggatacctt tcttctagct agggatgaaa atcaactttt aggctatatt
    27421 ttatcaagtc cccagtcaga caatccgcaa tatctaaaag tacattcttt agtcatcgag
    27481 tctgaccatc agagacaggg cttgggaaca cttcttcttg cagccttgaa agaggtggca
    27541 gttgagctgg attacaaagg gattcgtttg gagagtcctg atgagctgct ttcctatttt
    27601 gaaatgaacg gttttgttga tgaagaagca actttgctct atgcaactag ccagggctat
    27661 agtatgattt ggtttaatcc cttttatctg gaggaacaat gaaaatcaga caagcaagat
    27721 tagaagattt ggatcggatt gttgaacttg aatttgaaaa tttttcggtc gaagaagcca
    27781 ttcctccttc tgtctttgaa gcacatttga gagaaattca gacctctttt ctggttgctg
    27841 aaaaagaagg aagaatcatg ggttatatcg aaggaccagt tggcctgcac cgccatctgc
    27901 aagaccagtc ttttacagaa gaaataaaag actatagtca tgagcctggt ggttatatct
    27961 ctgtgacctg tctgtctatt gccaaggagg cacagggact cggactgggt cagaaattgc
    28021 tgacagcctt gaaagaagtt gctcttgaag atgagagaga tggcattaat ctaacctgtc
    28081 atgactatct catcgcctac tatgaaaaac atggatttgt caacgaaggc cagtcccagt
    28141 caacctttgc aggggaaaca tggtatgata tggtctggga aatgaaaaaa taagttagga
    28201 aaagtatcat agattgcttt ttttggactt ttaagatata atattatgga ttatcaacaa
    28261 ggaggaaaaa cttttgagtg aaaagtcaag agaagaagag aaattaagct ttaaagagca
    28321 gattctgaga gatttagaaa aagtaaaagg ctatgatgaa gttctgaaag aagatgaggc
    28381 agtagttcgc actcctgcaa atgaaccttc agctgaagaa ctcatggctg attccttgtc
    28441 aacggtagag gagattatga gaaaagctcc taccgtgtct actcacccaa gtcaaggtgt
    28501 accagcttct ccagcagatg agattcaaag agaaactcct ggtgttccaa gtcatccaag
    28561 tcaagatgta ccttcttctc cagcggaaga aagtggatca agaccaggtc caggtcctgt
    28621 tagacctaag aaacttgaaa gagaatacaa tgaaacccca acaagggtag ctgtttccta
    28681 tacgacggca gagaaaaaag cagaacaagc aggtccagaa acacctacgc ctgctacaga
    28741 aacagtggat atcatcagcg atacatcacg tcgtagccgt agagaaggag caaaacccgt
    28801 taatcctaag aaagagaaga agtcacatgt gaaagctttt gtgatttcat tccttgtatt
    28861 ccttgccttg ctctcagcag gtggttactt tggttaccag tacgtgctag attctttgtt
    28921 acctatcgat gctaattcta agaaatatgt gacggttgga attccagaag gttcaaacgt
    28981 tcaagaaatc ggtacgacgc ttgaaaaagc tggtttggta aagcatggtc tgatttttag
    29041 tttttatgcc aagtataaaa attataccga cttgaaagca ggttactaca atttgcaaaa
    29101 gagtatgagt acagaagact tactcaaaga gttgcaaaaa ggtggaacag atgaaccgca
    29161 agaacctgta cttgcgactt tgacaattcc agaaggttat accttggatc agattgctca
    29221 agctgtgggt caattgcaag gtgacttcaa agagtctttg acagcggagg ctttcttggc
    29281 taaagttcaa gatgagacgt ttatcagtca agcagtagcg aaatatccta ctttactgga
    29341 aagtttgcct gtaaaagaca gcggtgcgcg ttatcgtttg gaaggatacc ttttcccagc
    29401 tacatactct atcaaggaaa gcacaactat tgagagcttg attgatgaga tgttagctgc
    29461 tatggataag aacctatctc cttactatag tactatcaaa tctaaaaatt tgactgtcaa
    29521 tgagttgttg accattgctt ccttggtcga aaaagaaggt gccaagacag aagatcgtaa
    29581 gctcattgca ggtgtattct acaatcgttt aaatcgtgat atgccacttc aaagtaatat
    29641 tgcaatcttg tatgcccaag gaaaactggg gcaaaatatc agtctagctg aggatgttgc
    29701 gattgatacc aacattgatt caccttataa tgtttataaa aatgtaggtc tcatgcctgg
    29761 tccagtcgat agtccaagtc tggatgcgat tgagtcaagc atcaatcaaa ctaagagcga
    29821 taacctctac tttgtagcag atgtcacaga aggcaaggtc tactatgcta acaatcaaga
    29881 agaccacgac cgcaatgtcg ctgaacatgt caacagcaaa ttaaactaaa caaactaaaa
    29941 ttatgtgata cgtcacataa ttttctttag aaaatattat cagaagaaag ttgagaaaaa
    30001 tggcagaaaa aacatatcct atgacccttg aggaaaagga gaaacttgaa aaagaattag
    30061 aagaattgaa attggttcgt cgaccagaag tggtagaacg cattaagatt gcccgttcat
    30121 acggtgatct ttcagaaaac agtgagtacg aagcagctaa ggatgaacaa gcctttgtcg
    30181 aaggacaaat ctctagctta gaaacaaaaa tccgctatgc tgaaatcgtc aatagcgacg
    30241 cagttgccca ggacgaagta gcgattggta aaacagtcac catccaagaa attggcgagg
    30301 acgaagaaga agtttatatt atcgtaggtt cagctggtgc ggatgccttt gcaggtaagg
    30361 tttcaaatga aagcccaatt gggcaggcct tgattggcaa gaaaacaggt gacacagcaa
    30421 caattgaaac gcctgttggt agctatgatg taaaaatctt gaaggttgaa aaaacagcct
    30481 aaaaacagaa aaaggagtgg ggaggcgatg tgcttcactc actccttttt ccattttgct
    30541 actcttcgaa aatctcttca aaccacgtca gcgtcgcctt gccgtatata tggttactga
    30601 cttcgtcagt ttcatctaca acctcaaaac catgttttga gctgacttcg tcagttctat
    30661 ctacaacctc aaaaccatgt tttgagctga cttcgtcagt tctatctaca acctcaaaac
    30721 agtgttttga gcaacctgcg gctagtttcc tagtttgctc tttgattttc attgagtatt
    30781 agatgtatag tgcattgaag ttaaaatagt aaactttgat ttctaagaca ttattagaaa
    30841 ttagattaaa ttccttaatc gatttgcgca tgttttattt catttcatta tactttagat
    30901 tggaaaataa ggaatcacat aaatcgcaag aaatagggaa tctgattcgt gtttataacc
    30961 gttcaaaaag ggaagaggct gaaagtgagc cacttaatct ttatgtcgaa gatgaaaagg
    31021 gcaatctcct tgcgggtttg atagcagaga cttttggaaa ttggctagaa atcgagtatt
    31081 tgtttgtaaa agaggaactg cgagggcaag gaatcggttc aaaactattg caacaggcag
    31141 aaagtgaagc taagaatcga aattgttgtt ttgcctttgt caatacttat cagttccagg
    31201 caccggactt ttatcagaaa catggctaca aggaagtttt ttctttgcaa gactatctct
    31261 acattaggca aagatattat taccaaaaga atctgtaagg tgagtatgga ggtataatac
    31321 aaagagaggc tcgcacataa gtatggaggt ggactgatgt atatgagtaa ctatcgataa
    31381 ataccagtta taacctagaa attgtgtctt tctagacgat attgaggaca atataatcgt
    31441 agctccaaaa ttaggaatca aggcttatca ggttaagaaa agaagtgatg ttgttgatat
    31501 tttaaaacaa tttatttaaa ctaaaaactc tctatctttt taaattgaca ttaatccgag
    31561 attcccctct cggattttgt gttgctttca ttaaatttac aggtaagact tgctagcttc
    31621 agtaaatata cacaagtttt tcagaataga gtgaaatccg aaagaaaatg gaaataatga
    31681 aagctcttgt aggcataaga aaacacctct gtgttatact tgttgttcaa ccacaaacac
    31741 aagaaaggca cagagatgca aaaccattat actacaaaag gcgaacaaag ggaaatcaaa
    31801 tagagaaatt gcctctctgc ttggaaaagc tcctcaaact atccacacta aaatcaagcg
    31861 taggacagtc cgaaaatgtc ttggaaaagg gcgcttcaaa gaggtttatt ctgccgacta
    31921 tgctcaacag tcttatgaaa ataatcgcaa gcactcggtc aagagatcaa gcgtgaccaa
    31981 ggaactaaag gaaaagattc tccactatca taaccaaaaa tttttgcctg aaatgatggt
    32041 tatggctaaa ggggttaacg tgggaatttc aaccatttac tattggattc atcatggaaa
    32101 attggggtaa agcaagcagg atctgcttta ccctagaaaa ggaaaagcgc ttaagaaaca
    32161 ggccagcacc aactttaaac ctgctggtca atccatcgaa cagcggcctg aagctatcaa
    32221 tcttcgtttg gagaatgggc attatgagat tgatacggtt ctacttacga gagcgaaaaa
    32281 ctactgcttg cttgtcttga cggatcgaaa gagtagacat cagatcatcc gattgattcc
    32341 aaataaaagt gctgaggtgg tcaatcaggc tctaaaactc atcttaaaac aacacaagat
    32401 tctttccatc acggcagata atggaacgga attcaatcgc ttgtctaatg tattttctga
    32461 ggagcacatc tattatgcgc acccctatgc ctcttgggaa aggggaacta atgagaatca
    32521 caacaggctc attcgtagat ggttacctaa gggaaccaag aaaatgactc ccaaagaagt
    32581 cgcattcatc gaaaagtgga ttaacaacta tcctaaaaaa tgcttggact acaagtcacc
    32641 cagagaagac ttctggatgg ctaacttgaa cttgaaattt agcaaaatgg aaataatttt
    32701 tattaaacgc ttccaatagc ggttaaaagt tgacaaattt aaattttagg actacactaa
    32761 gtaagtaaat tttattttaa aggagaattt gttatgaatt taacattttt aggcttatgt
    32821 attgcctgta tgggcgtatc tgtcggtgaa ggtttattga tgaatggact gtttaaatca
    32881 gtagcacgcc aaccagatat gctttctgag tttcgtagtt tgatgttttt aggtgttgcc
    32941 tttattgaag gaactttctt tgtaactctt gtcttctcat ttattatcaa ataaatacat
    33001 ggaacgagaa gaaaagggag gattttagat ggaagaaagt attaatccaa tcatctctat
    33061 tggtcctgtt atcttcaatc tgactatgtt agccatgact ttgttgattg tgggagttat
    33121 ttttgtcttt atttattggg caagccgcaa tatgaccttg aaacccaaag gaaagcaaaa
    33181 tgtacttgag tatgtctatg actttgttat tggatttaca gaacctaaca ttggttcgcg
    33241 ctacatgaaa gattactcac tctttttcct ttgtttattc cttttcatgg tgattgccaa
    33301 taaccttggc ttaatgacaa agattcaaac gatcgatggg actaactggt ggagttcgcc
    33361 aaccgctaat ttacagtatg acttaacctt atcttttctt gtcattttgt tgacacatat
    33421 agaaagcgtt cgtcgtcgtg gatttaaaaa aagtataaaa tcttttatga gtcctgtttt
    33481 tgtcataccg atgaatatct tggaagaatt tacaaacttc ttatctttgg ctttgcggat
    33541 ttttgggaat atctttgcag gagaggtcat gacgagtttg ttacttcttc tttcccacca
    33601 agctatttat tggtatccag tagcctttgg agctaatttg gcttggactg cattttctgt
    33661 ctttatttcc tgcatccaag cttatgtttt tactcttttg acatctgtgt atttagggaa
    33721 taagattaat attgaagagg aatagaaagg agtaactgat gcacgtaaca gtaggtgaat
    33781 taattggtaa ttttatttta atcactggct cttttattct tttgctagtc ttgattaaaa
    33841 aatttgcatg gtctaatatt acaggcattt tcgaagaaag agctgaaaaa attgcttcag
    33901 atattgacag agctgaagaa gcccgtcaaa aagcagaagt attggctcaa aaacgcgaag
    33961 atgaattggc tggtagccgt aaagaagcta agacaatcat tgaaaatgca aaggaaacag
    34021 ctgagcaaag taaggctaat atcttagcag atgctaaact agaagcagga cacttaaaag
    34081 aaaaagccaa tcaagaaatt gctcaaaata aagtagaagc tttacagagt gttaagggtg
    34141 aggtcgcaga tttgaccatc agcttagctg gtaaaatcat ctcacaaaac cttgacagtc
    34201 atgcccataa agcactcatt gatcagtata tcgatcagct aggagaagct taatggacaa
    34261 gaaaacagta aaggtaattg aaaaatacag catgcctttt gtccaattgg tacttgaaaa
    34321 aggagaagaa gaccgtatct tttcagactt gactcaaatc aagcaagttg ttgaaaaaac
    34381 aggtctgcct tcttttttaa aacaagtggc agtagacgag tcggataagg aaaaaacaat
    34441 tgcttttttc caagattctg tgtcgccttt attacaaaac tttatccagg ttctggccta
    34501 caatcacaga gcaaatcttt tttatgatat gcttgtagat tgcttgaacc gacttgaaaa
    34561 agaaacaaat cgatttgaag tgacgattac gtctgctcat cctctaactg atgaacagaa
    34621 gactcgtttg ctccctttga ttgagaaaaa aatgtctctg aaagtaagga gtgtaaaaga
    34681 acaaatcgat gaaagtctca ttggtggttt tgtcattttt gccaatcaca agacaattga
    34741 tgtgagtatt aaacaacaac ttaaagttgt taaagaaaat ttgaaataga aagtggtgtt
    34801 cttttggcaa ttaacgcaca agaaatcagc gctttaatta agcaacaaat tgaaaatttc
    34861 aaacccaatt ttgatgtgac tgaaacaggt gttgtaacct atatcgggga cggtatcgcg
    34921 cgtgctcatg gccttgaaaa tgtcatgagt ggagagttgt tgaattttga aaacggctct
    34981 tatggtatgg ctcaaaactt ggagtcaaca gacgttggta ttatcatcct aggtgacttt
    35041 acagatatcc gtgaaggcga tacaatccgc cgtacaggga aaatcatgga agtccctgta
    35101 ggtgaaagtc tgattggtcg tgttgtggat ccgcttggtc gtccagttga cggtcttgga
    35161 gaaatccaca ctgataaaac tcgtccagta gaagcaccag ctcctggtgt tatgcaacgt
    35221 aagtctgttt cagaaccatt gcaaactggt ttgaaagcta ttgacgccct tgtaccgatt
    35281 ggtcgtggtc aacgtgagtt gattatcggt gaccgtcaga cagggaaaac aaccattgcg
    35341 attgatacaa tcttgaacca aaaagatcaa gatatgatct gtatctacgt cgcgattgga
    35401 caaaaagaat caacagttcg tacgcaagta gaaacacttc gtcagtacgg tgccttggac
    35461 tacacaatcg ttgtgacagc ctctgcttca caaccatctc cattgctctt cctagctcct
    35521 tatactgggg ttgctatggc ggaagaattt atgtatcaag gtaagcatgt tttgattgta
    35581 tacgatgatc tatcaaaaca agcggtagct tatcgtgaac tatcgctctt gcttcgtcgt
    35641 cctccaggtc gtgaagcctt cccaggggat gttttctatc tccacagccg tttgcttgag
    35701 cgctcagcta aagtttctga tgaacttggt ggtggatcaa ttacagccct accatttatc
    35761 gagacacaag caggagatat ctcagcctat atcgcaacca acgtgatttc tatcactgat
    35821 ggacaaatct tccttggcga tggcctcttc aatgcaggta ttcgtccagc catcgatgcg
    35881 ggttcatctg tatctcgtgt aggtggttct gcacaaatca aagccatgaa gaaggttgct
    35941 ggtacacttc gtatcgacct tgcttcatac cgtgagttag aagcctttac taagtttggt
    36001 tctgacttgg acgcagcaac acaggctaag ttgaaccgtg gacgtcgtac cgttgaggtc
    36061 ttgaaacaac ctgttcacaa accattacct gttgagaaac aagtaaccat tctttatgct
    36121 ttgacacatg gtttcttgga tactgttcca gtagatgata ttgttcgttt cgaggaagag
    36181 ttccatgcct tctttgatgc tcaacatcca gagattttgg aaaccattcg tgatacaaaa
    36241 gacttgccag aagaagcagt cttggatgct gcgattacag agtttctcaa tcaatctagc
    36301 ttccaataag aatagaggtg tcagatggca gtgtctctaa atgatattaa aacaaaaatc
    36361 gcctcaacaa aaaatacgag tcaaatcact aatgccatgc aaatggtatc ggctgctaag
    36421 ctaggtcgct ctgaagaagc tgctcgcaac ttccaagttt acgctcagaa agtgcgtaaa
    36481 cttttgacag atatccttca tggtaatgga gctggtgctt caactaatcc gatgttgatt
    36541 agtcgttctg tgaagaagac aggctatatc gttatcactt cagaccgcgg tttggttgga
    36601 ggttataatt cctctatttt gaaagctgtt atggagttga aagaagaata ccacccagac
    36661 ggtaaaggtt ttgaaatgat ctgtatcggt gggatgggag ctgatttctt taaggctcgc
    36721 ggtattcaac cactttatga attacgtggc ttggcagacc aacctagctt tgatcaagtt
    36781 cgtaagatta tttcaaaaac tgttgaaatg taccaaaatg aactctttga tgagctttat
    36841 gtttgctaca accaccatgt caatacgcta accagtcaaa tgcgtgtgga acaaatgctt
    36901 ccgattgttg acttggatcc aaatgaagcg gatgaagagt acagcttgac ttttgaattg
    36961 gaaaccagcc gagaagaaat tctggagcag ttgttgcctc agtttgcaga aagtatgatt
    37021 tacggtgcca ttatcgatgc caagacagct gagaatgctg cgggtatgac agccatgcaa
    37081 acagcgacag ataatgctaa gaaagtcatc aatgatttga caattcagta taaccgtgcc
    37141 agacaggcgg cgattacaca agaaattaca gaaatcgtag caggtgctag tgccttagaa
    37201 taggctctag tccagctcgt atgaaaatga acttaggacc tagttgagct aggaaccgac
    37261 agtatcttat atagaatagg agaaggagat gagttcaggt aaaattgctc aggttatcgg
    37321 tcccgttgta gacgttttgt ttgcagcagg ggaaaaactt cctgagatta acaatgcact
    37381 tgtcgtctac aaaaatgacg aaagaaaaac aaaaatcgtc cttgaagtag ccttggagtt
    37441 aggagatggt atggttcgta ctatcgccat ggaatcaaca gatgggttga ctcgtggaat
    37501 ggaagtattg gacacaggtc gtccaatctc tgtaccagta ggtaaagaaa ctttgggacg
    37561 tgtcttcaac gttttgggag ataccattga tttggaagct ccttttacag aagacgcaga
    37621 gcgtcagcca attcataaaa aagctccaac ttttgatgag ttgtctacct cttctgaaat
    37681 ccttgaaaca gggatcaagg ttattgacct tcttgcccct taccttaaag gtggtaaagt
    37741 tggacttttc ggtggtgccg gagttggtaa aaccgtctta atccaagaat tgattcacaa
    37801 cattgcccaa gagcacggtg gtatttcagt atttactggt gttggggaac gtactcgtga
    37861 ggggaatgac ctttactggg aaatgaaaga atcaggcgtt atcgagaaaa cagccatggt
    37921 ctttggtcag atgaatgagc caccaggagc acgtatgcgt gttgccctta ctggtttgac
    37981 aatcgctgaa tacttccgtg atgtggaagg ccaagacgtg cttctcttta tcgataatat
    38041 cttccgtttc actcaggctg gttcagaagt atctgccctt ttgggtcgta tgccatcagc
    38101 cgttggttac caaccaacac ttgctacgga aatgggtcaa ttgcaagaac gtatcacatc
    38161 aaccaagaag ggttctgtaa cctctatcca ggctatctat gtgccagcgg atgactatac
    38221 tgacccagcg ccagcaacag ccttcgctca cttggattca acaacaaact tggaacgtaa
    38281 attggtacaa ttgggtatct acccagccgt tgacccactt gcttcaagct cacgtgcctt
    38341 ggcacctgaa atcgttggag aagagcacta tgcagttgct gctgaagtaa aacgtgtcct
    38401 tcaacgttac catgaattgc aagatatcat tgctatcctt ggtatggatg agctttctga
    38461 tgaagaaaag accttggttg ctcgcgcccg tcgtatccag ttcttcttgt cacaaaactt
    38521 caacgttgcg gaacaattta ctggtcagcc aggttcttat gttccagttg ctgaaactgt
    38581 acgtggcttt aaggaaatcc ttgatggtaa atacgaccac ttgccagaag atgccttccg
    38641 tggtgtaggt tctatcgaag atgtgattgc aaaagctgaa aaaatgggat tttaagaggt
    38701 gacctatggc tcagttaact gtccagatcg tgacaccaga tggtctcgtc tatgatcacc
    38761 atgccagcta tgtatcggtt cgaactctgg atggtgagat ggggatcttg ccacgacatg
    38821 aaaatatgat tgcggtttta gcagttgatg aagtaaaggt aaaacgtatc gatgataaag
    38881 atcacgtgaa ctggattgca gtaaacggtg gcgttattga aattgccaat gatatgatca
    38941 caatcgtcgc tgactctgca gaacgtgctc gtgatatcga tattagccgt gcagaacgtg
    39001 ccaaacttcg tgcagaacgt gcaattgaag aagcacaaga caaacatttg attgaccaag
    39061 aacgtcgtgc taagattgct ttgcaacgtg ccattaaccg tattaatgtc ggaaatagac
    39121 tataagaaaa aatgaacttg aaaataccaa gttcattttt tatggtgttt taaggagcaa
    39181 aacggatgca gactgcttcg ggaacatgga agtcgttgga gagttctgct agacgaccat
    39241 tgtcacaatt acgtttaaag acagttgcat tgtcagagtc ttgatggaca acaatgagaa
    39301 atttttggtc gggtgtcaaa tcaaaatcac gtggagtctg accatgcgtt ggaacgattt
    39361 ctaataactc taagctaccg tccgcaagga tggtatatac tgcgatagaa tcatggccac
    39421 ggttagaagc gtagaggtat ttaccgtctt tagagagatg aatagcagcg gttccattaa
    39481 agccttcgta agcttctggt aaagttgaaa taacctgcat acgttcaaat tcgccaacgc
    39541 catcgtagat taaaacttcg atagtactat tgagttcaca aatgagataa gcgattttat
    39601 agtggttatg gaaaatgata tggcgtgagc ctgctcctgg cttgctgtga taggtataga
    39661 gcttagataa ttttccttct tgatcgaggt cataggtgat gacttggtca gttcccaagt
    39721 cgcaggtcac tagatagtgg tcaggtgtta aatctgtata gtgaacatgg ggggaagctt
    39781 gattttcatg tggaccttgg ccactgtgtt gatccatatc actaagtaga agactaccat
    39841 cttcctggcg tttataaaca aggacttgtc ccttgtgata gttggctgcg taaaccaaat
    39901 cacgcttttc atcgacagca acataacagt ggggagctcc ttcttcaaca acatgattta
    39961 acacagtccc gtcagtttga taggctgcaa ttcccccctt atcgtcttgg ctaccaacag
    40021 tgtataaatg ttggtgctgg tcaaaggcaa ggtaggttgg actcggctca gctgcaaaaa
    40081 gttctagatt tgaaagctga ccagtttctg tatcaaagtc tgccttgtaa atcccttgag
    40141 aagtacgacg tgtataagtt ccaaaataaa cagtttcttt cattactata cctctgtgta
    40201 aagataagac tattatatca
//
LOCUS       DAVKGU010000008        38125 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKGU010000008 DAVKGU010000000
VERSION     DAVKGU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38125
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.008"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            86..1111
                     /gene="trpS"
                     /locus_tag="VW077_000398"
     CDS             86..1111
                     /gene="trpS"
                     /locus_tag="VW077_000398"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9454479.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFTKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1263..2741
                     /gene="guaB"
                     /locus_tag="VW077_000399"
     CDS             1263..2741
                     /gene="guaB"
                     /locus_tag="VW077_000399"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9454480.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2792..3889)
                     /gene="recF"
                     /locus_tag="VW077_000400"
     CDS             complement(2792..3889)
                     /gene="recF"
                     /locus_tag="VW077_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9454481.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNRN"
     gene            complement(3892..4260)
                     /locus_tag="VW077_000401"
     CDS             complement(3892..4260)
                     /locus_tag="VW077_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9454482.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SCRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4419..5669
                     /locus_tag="VW077_000402"
     CDS             4419..5669
                     /locus_tag="VW077_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9454483.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSEFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGISSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            5666..6949
                     /locus_tag="VW077_000403"
     CDS             5666..6949
                     /locus_tag="VW077_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9454484.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            6991..7812
                     /locus_tag="VW077_000404"
     CDS             6991..7812
                     /locus_tag="VW077_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9454485.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7823..8368
                     /gene="pgsA"
                     /locus_tag="VW077_000405"
     CDS             7823..8368
                     /gene="pgsA"
                     /locus_tag="VW077_000405"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9454486.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8365..9192
                     /locus_tag="VW077_000406"
     CDS             8365..9192
                     /locus_tag="VW077_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9454487.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPGELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9177..10016
                     /locus_tag="VW077_000407"
     CDS             9177..10016
                     /locus_tag="VW077_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9454488.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10009..10803
                     /locus_tag="VW077_000408"
     CDS             10009..10803
                     /locus_tag="VW077_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9454489.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10862..11680
                     /gene="mreC"
                     /locus_tag="VW077_000409"
     CDS             10862..11680
                     /gene="mreC"
                     /locus_tag="VW077_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9454490.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11680..12174
                     /gene="mreD"
                     /locus_tag="VW077_000410"
     CDS             11680..12174
                     /gene="mreD"
                     /locus_tag="VW077_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9454491.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12268..13446
                     /locus_tag="VW077_000411"
     CDS             12268..13446
                     /locus_tag="VW077_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9454492.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13670..14449
                     /gene="rpsB"
                     /locus_tag="VW077_000412"
     CDS             13670..14449
                     /gene="rpsB"
                     /locus_tag="VW077_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9454493.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14528..15568
                     /locus_tag="VW077_000413"
     CDS             14528..15568
                     /locus_tag="VW077_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9454494.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEDGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15815..16138
                     /gene="tnpB"
                     /locus_tag="VW077_000414"
                     /pseudo
     CDS             15815..16138
                     /gene="tnpB"
                     /locus_tag="VW077_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(16279..16587)
                     /locus_tag="VW077_000415"
                     /pseudo
     CDS             complement(16279..16587)
                     /locus_tag="VW077_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16578..16976
                     /locus_tag="VW077_000416"
     CDS             16578..16976
                     /locus_tag="VW077_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9454495.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(17173..18093)
                     /gene="cysK"
                     /locus_tag="VW077_000417"
     CDS             complement(17173..18093)
                     /gene="cysK"
                     /locus_tag="VW077_000417"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9454496.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(18378..19013)
                     /locus_tag="VW077_000418"
     CDS             complement(18378..19013)
                     /locus_tag="VW077_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9454497.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQIDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            19069..20367
                     /locus_tag="VW077_000419"
     CDS             19069..20367
                     /locus_tag="VW077_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9454498.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     SIILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKATKEIQMMNKEAGL"
     gene            20364..21026
                     /locus_tag="VW077_000420"
     CDS             20364..21026
                     /locus_tag="VW077_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9454499.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            21106..21654
                     /gene="raiA"
                     /locus_tag="VW077_000421"
     CDS             21106..21654
                     /gene="raiA"
                     /locus_tag="VW077_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9454500.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            21790..23763
                     /locus_tag="VW077_000422"
     CDS             21790..23763
                     /locus_tag="VW077_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9454501.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDETSESDISQINSFVANFISEFSEKHMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            23760..24212
                     /gene="rplI"
                     /locus_tag="VW077_000423"
     CDS             23760..24212
                     /gene="rplI"
                     /locus_tag="VW077_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9454502.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            24256..25608
                     /gene="dnaB"
                     /locus_tag="VW077_000424"
     CDS             24256..25608
                     /gene="dnaB"
                     /locus_tag="VW077_000424"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9454503.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            25610..25876
                     /locus_tag="VW077_000425"
     CDS             25610..25876
                     /locus_tag="VW077_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454504.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            25965..27311
                     /gene="cbpD"
                     /locus_tag="VW077_000426"
     CDS             25965..27311
                     /gene="cbpD"
                     /locus_tag="VW077_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9454505.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGEMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      27394..27496
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27567..27857
                     /locus_tag="VW077_000427"
     CDS             27567..27857
                     /locus_tag="VW077_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9454506.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVPMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            27820..28578
                     /locus_tag="VW077_000428"
     CDS             27820..28578
                     /locus_tag="VW077_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454507.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            28617..29624
                     /locus_tag="VW077_000429"
     CDS             28617..29624
                     /locus_tag="VW077_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9454508.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            29624..30352
                     /locus_tag="VW077_000430"
     CDS             29624..30352
                     /locus_tag="VW077_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454509.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            30471..30929
                     /locus_tag="VW077_000431"
     CDS             30471..30929
                     /locus_tag="VW077_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9454510.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            30931..33363
                     /locus_tag="VW077_000432"
     CDS             30931..33363
                     /locus_tag="VW077_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9454511.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSILKI
                     GVKAGQLKFDIA"
     gene            33684..34337
                     /gene="rr06"
                     /locus_tag="VW077_000433"
     CDS             33684..34337
                     /gene="rr06"
                     /locus_tag="VW077_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9454512.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            34334..35665
                     /gene="hk06"
                     /locus_tag="VW077_000434"
     CDS             34334..35665
                     /gene="hk06"
                     /locus_tag="VW077_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9454513.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            35760..36287
                     /locus_tag="VW077_000435"
     CDS             35760..36287
                     /locus_tag="VW077_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9454514.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            36366..>38125
                     /gene="pspC"
                     /locus_tag="VW077_000436"
     CDS             36366..>38125
                     /gene="pspC"
                     /locus_tag="VW077_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU9454515.1"
                     /translation="MFASKNERKVHYSIRKFSIGVASVAVASLFMGSVVHATEKEVTT
                     QVATSSNKANKSQTEHMKAAKQVDEYIKKKLQLDRRKHTQNVGLLTKLGVIKTEYLHG
                     LSVSKKKSEAELPSEIKAKLDAAFEQFKKDTLPTEPGKKVAEAEKKVEEAKKKAEDQK
                     EKDLRNYPTNTYKTLELDIAESDVEVKKAELELVKEEAKESRDEKKINQAKAKVENKK
                     AEATRLKNIKTDREKAEEAKRRADAKLQEANVATSEQDKSKRRAKREVLGELATPDKK
                     ENDAKSSDSSVGEETLTSPSLKPEKKVAEAEKKVEEAKKKAEDQKEEDRRNYPTNTYK
                     TLELEIAESDVEVKKAELELVKEEAKESRNEEKIKQVKAKVESKKAEATRLENIKTDR
                     KKAEEEEAKRRAAEEDKVKEKPAEQPQPAPAPQPEKPTEEPENPAPAPAPKPENPAEK
                     PKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKAEKPAQPSTPKTGWKQENGMWYFYN
                     TDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYY
                     LNANGSMATGWLQYNGSWYYL"
BASE COUNT        11994 a         6223 c         8413 g        11495 t
ORIGIN      
        1 agcaaggctg atttattttg ataaattacg gttatttcat taaaaaaatg ctataattga
       61 aaggactata tcgaaggaga acaaaatgac taaacccatt attttaacag gagaccgtcc
      121 aacaggaaaa ttgcatattg gacattatgt tggaagtctc aaaaatcgag tattattaca
      181 ggaagaggat aagtatgata tgtttgtgtt cttggctgac caacaagcct tgacagatca
      241 tgccaaagat cctcaaacca ttgtagagtc tatcggaaat gtggctttgg attatcttgc
      301 agttggattg gatccaaata agtcaactat ttttattcaa agccagattc cagagttggc
      361 tgagttgtct atgtattata tgaatctagt ttcgttagca cgtttggagc gaaatccaac
      421 agtcaagaca gagatttctc agaaaggatt tggagaaagc attccgacag gattcttggt
      481 ctatccaatc gctcaagcag ctgatatcac agctttcaag gctaattatg ttcctgttgg
      541 gacagatcag aaaccaatga ttgagcaaac tcgtgaaatt gttcgttctt ttaacaatgc
      601 atataactgt gatgtcttgg tagagccgga aggtatttat ccagaaaatg agagagcagg
      661 gcgtttgcct ggtttagatg gaaatgctaa aatgtctaaa tcactaaata atggtattta
      721 tttagctgat gatgcggata ctttgcgtaa aaaagtaatg agtatgtata cagatccaga
      781 tcatatccgc gttgaggatc caggtaagat tgagggaaat atggttttcc attatctaga
      841 tgtttttggt cgtccagaag atgctcaaga aattgctgat atgaaagaac gttatcaacg
      901 aggtggtctt ggtgatgtga agaccaagcg ttatctactt gaaatattag aacgtgaact
      961 gggtcctatt cgtgagcgcc gtattgaatt tactaaggat atgggagaag tttataatat
     1021 gattcaaaaa ggtagtgaaa gagcgcgtga agttgcgggt caaaccctat ctgaggtaaa
     1081 aggggcaatg ggacttcatt actttaacta agtttatttt acaagaaact atcttttcta
     1141 tctcgaagaa aagatagttt tttatttacc cctgtaacat ttgacaaatt tttatagaat
     1201 ggtatgatag atacaatata aaaagagtca agctcaaaaa gaaagaaaag aggaaacttc
     1261 gaatgtctaa ttgggacact aaatttttga aaaaaggttt tacctttgat gatgtattgc
     1321 ttattccagc tgaaagtcat gtgttgccta acgatgcaga tttaacaact aaattggcag
     1381 ataatttgac tttaaatatc ccaattatta ccgctgccat ggacacagtt acagagagtc
     1441 aaatggccat tgctattgct cgtgcaggcg gtctcggagt tatccataaa aacatgtcaa
     1501 ttgctcaaca agcagacgag gttcgtaagg taaaacgttc tgaaaatgga gttattattg
     1561 atccgttctt cttgacgcct gaacatacaa ttgctgaagc agatgagctt atgggtcgtt
     1621 accgcatcag tggtgttcca gttgttgaaa cacttgaaaa tcgtaaattg gttggtattt
     1681 tgacaaaccg agatcttcgt tttatttcag attataatca accaatttca aaccatatga
     1741 ctagtgaaaa tcttgttact gctcctgtgg gtacggatct tgcaacggct gagagtattc
     1801 ttcaagagca tcgtattgaa aaacttccgt tggtcgatga agaaggcagt ctttctggtt
     1861 tgatcactat caaagatatt gaaaaagtta ttgagtttcc aaatgcggct aaagatgagt
     1921 ttggtcgtct tctagttgca ggtgcagtag gtgttacttc agatacattt gaacgtgcag
     1981 aggctctttt tgaggcagga gcggatgcga ttgttattga tactgcacat ggtcattctg
     2041 caggtgtctt gcgtaaaatt gccgagattc gtgctcattt cccagatcgg actttgattg
     2101 ctggaaatat tgctactgct gaaggtgcac gtgcccttta tgaagcgggt gtagacgttg
     2161 ttaaggttgg tattggacca ggttctatct gtactactcg tgtgattgct ggtgttggtg
     2221 ttccgcaagt aacagctatc tacgatgctg cagctgttgc gcgcgaatat ggtaaaacga
     2281 ttattgctga cggtgggatc aagtattctg gagatattgt aaaagcactt gctgcaggtg
     2341 gaaatgctgt tatgcttgga tctatgtttg ctggaactga tgaagctcca ggcgaaactg
     2401 aaatcttcca aggacgtaaa ttcaagactt accgtggtat gggatcaatt gctgctatga
     2461 agaaaggttc aagcgaccgt tatttccaag gttctgtcaa tgaagcaaac aagcttgttc
     2521 cagaaggaat tgaaggtcgt gttgcttata aaggagcggc agctgatatt gttttccaaa
     2581 tgattggtgg tattcgctct ggtatgggtt actgtggtgc agctaacctt aaagaactac
     2641 acgataatgc tcaatttatt gaaatgtctg gtgctggttt gaaagaaagc catcctcatg
     2701 atgtgcaaat tactaatgag gcaccaaatt attctatgta aaaaacaatg aaaagaactc
     2761 cagtgaaaac aggagttctt ttacaatgtt gtcaatttct atttacagca gctttaccat
     2821 cctgaatagt gaagatactt agattttctg gcagattttg aagatggtct aagcttgttg
     2881 ttgtgataaa ggtttggatt gattgagaaa tcgtttctaa taattttaac tgtctagtgt
     2941 tgtcaagttc actcatcaca tcgtcaagca gtaatatcgg agattctgtg gtaatgcttt
     3001 ccattaattc gatttctgct aattttatcg agaggacgag actacgatgt tgaccttggc
     3061 ttccgaaact agcatccatc ccatttatat aaaaagaaat gtcatctcga tgaggaccga
     3121 caccagtatt ctttttaaat aaatctctgg atctactttt ttctaaagca attttgaaag
     3181 attcggataa gttttgtttg tcagttatat tgacagaaga ttgataggat attgacaact
     3241 cttcgatctg attagagagt tcaaaatgtt tcttacgccc aaatgattct agttttttta
     3301 tgaaatctaa gcggtgattc attacacgac atccataatc aactagctga tcatctaaca
     3361 cagaaaggaa tgtttcatct attttttgag ctgattttag gtaagtgttt ctttgcttta
     3421 ggatgtggtt ataattggtt aagtcagata aatagattgg cttaatttgc ccaagttcca
     3481 tatcaatgaa ttttcgtcga atcgaaggtg ctcctttaat tagttgtaaa tcttcaggag
     3541 caaataagac aacattcatg tgtcctacat aatctgaaag gcgtgcctgt tttaagtgat
     3601 taacttttgt tacacggcct ttttgtgtta gttcgatttc taggggaatg gatccagttt
     3661 ttttctgaac gagacctgaa agatgaagtt gttcctcatc aaaatgaatg agatttttat
     3721 ctgttcgagt tcgatgacta cgcgttaagg ctaaaaaata gatagcctct aacatgtttg
     3781 tttttccttg tgcattacgt cccaaaaaga cattcaattt aggattaaag tctattttcg
     3841 tttctttgta attacgaaaa gtcttgagag atagatgttg tagccacatg attatctacc
     3901 agggaatcgt ggagcttgtt ggcttttagg tgatgaagca ggtttcgatt tgtctttttt
     3961 aactccctta ttcatctttt tgacaagttt agcgatccgt tctttttcaa ctttatcagc
     4021 ttggtattca tcttgttctt cagaagtagg ttgtgtcaac aagatgtcaa tgttcatgtc
     4081 agggatgtca actttatcgc caatacgaag tttttttcca cgacaactct ctaattccct
     4141 attaaagtaa acagaatgtt cagagagaaa tgatttaata gctcctccgc tatgtgtaat
     4201 tccaagttct ttgagtagtg cttggagggt aataaattct tcaaataatt tgtattccat
     4261 aattcacctc aatctttggt attataccat attttcctaa aaatagtgag agagacagcg
     4321 agaaatgtaa gcgattttta tttcaaaagt gttacaggga acaagaaaat ttcaggtttt
     4381 cgtgatataa tagaagtctg tatataagga ggtaaatcat ggagttagtg catggaattt
     4441 caacacattt tatccaatca aaaaagttta aaacaaacaa aattaccgtg cgttttaccg
     4501 ctccattatc ccttgatacg attgcaggtc acatgttgag tgcaagtatg ctagagactg
     4561 ctaatcagat gtaccccact tctcaagatt tgaggagaca cttggccagt ctatacggta
     4621 cagatatgtc aaccaattgt ttcagaagag ggcaaagcca cattatagaa ttgacattta
     4681 cctatgttcg tgatgagttt ttaagtagga aaaacgtgct aacctctcag attttggaac
     4741 ttgtaaaaga aactcttttt tcacccgtag tagttgataa tgggtttgat ccggccttat
     4801 ttgaaattga gaaaaaacaa ttgctagcaa gtttagcagc tgatatggat gattcttttt
     4861 attttgcaca taaagaattg gataaattgt tttttcatga tgaacgtctt caattggaat
     4921 atagtgattt acgaaatcgt attttagctg aaactccaca aagttcttat tcttgtttcc
     4981 aagaattttt agccaatgat cgaatagatt tctttttcct aggtgatttt aatgaggttg
     5041 aaattcaaaa tgtattagaa tcatttggct ttaaaggtcg aaaaggagat gtgaaggttc
     5101 agtattgtca accttattct aatatccttc aggaaggtat ggttcggaaa aatgtgggac
     5161 aatccatttt ggaattaggt tatcattacc gttctaaata tggtgatgag caacatttac
     5221 ccatgattgt aatgaatggt ttacttggtg gatttgctca ctctaagctc tttacaaatg
     5281 tccgtgaaaa tgctggatta gcttatacca tttcaagtga gcttgattta tttagtgaat
     5341 tcttgaggat gtatgctggt atcaatcgag aaaatcgtaa ccaggctcgt aaaatgatga
     5401 ataatcaact gcttgattta aaaaaaggtt attttacaga gtttgagtta aatcagacca
     5461 aggaaatgat tcgttggtcg ttgttacttt ctcaagataa tcaatcttca ttgattgaac
     5521 gtgcttatca aaatgcctta tttggaatat cttcagcaga ctttaaaggt tggattgcaa
     5581 aactcgagca agttgacaaa gatgctattt gtagagcagc taataatgtg aaactacaag
     5641 cgatttactt tatggaagga atagaatgac aaaggttgtt tttgaagaaa aatactatcc
     5701 agctgtaaaa gaaaaggttt atcgaactcg tttggccaat ggattgacag ttgctctttt
     5761 gcctaaaaag gaatttaaag aggtttacgg gagtgtcact gtacagtttg gttcggtaga
     5821 tacgtttgtc acagaagttg acggagatgt aaaacaatat cctggaggaa ttgctcattt
     5881 tcttgaacat aaattatttg agagagaaga ttctagtgat ttgatgtcgg cttttacgag
     5941 tctaggtgca gatagtaatg cctttacaag ctttacaaaa acaaactatc ttttttcagc
     6001 aacggattat tttttagaaa atttagattt acttgatgaa ttggtaacat cagcacactt
     6061 tactgaagct tccattctga cagagcagga tattattcag caagaacgag aaatgtacca
     6121 agatgatcca gattcgtgtt tattcttttc aactttagcg aatttgtatc ctggtacacc
     6181 tttagcaact gatatagttg gaagtgagga gtccatttcc caaatcaatc taactaattt
     6241 gcaagaaaat tttacaaagt tttacaaacc tgtaaacatg tctctgtttt tagttggtaa
     6301 ttttgatgtg gagcgagtac aggactattt tgaaagcaaa gaactgaaag attcagattt
     6361 tcaggaagta gcaagagaaa agttgttttt acagcctgta aagccaacag atagtatgag
     6421 aatggaagta tcttctccca aactagcgat tggagttaga ggtaagcgag aagtttctga
     6481 agcggattgc tatcgacatc atattttatt aaaattattg tttgcaatga tgtttggttg
     6541 gacttcggat cgttttcaaa aatgttatga atcaggtaaa attgatgcat ccttatctct
     6601 ggaagttgaa gtaacaagtc gctttcattt tgtcatgttg acaatggata cgaaagagcc
     6661 agttgctttg tctcatcaat ttaggaaggc tattcgtaat tttacaaagg atttagatat
     6721 tacagaggaa catttagata ttatcaaaag agagatgttt ggcgaatttt tcagtagcat
     6781 gaactctctt gaatttattg caacgcaata tgatgctttt gaaaatggtg agacaatttt
     6841 tgatttgccg aaaattttac aggaaattac tttagaggat gtccttgatg ctggacatca
     6901 tttaatagat gatggtgaca tagttgattt tacaatattc ccatcgtagt aacctattat
     6961 aatagacact agaaagaagg gatgacaagt atgagaaaaa aaacaattgg agaggtttta
     7021 cgattagcta gaatcaatca gggattgagt ttagatgaat tgcagaaaaa gacagaaatc
     7081 cagttagata tgttggaagc aatggaagca gacgatttcg atcaacttcc aagtcctttt
     7141 tacacgcgtt ctttcttgaa aaaatatgca tgggctgttg agttagatga ccaaattgtt
     7201 ttggatgctt atgattctgg gagtatgatt acttatgagg aagtagatgt tgatgaagat
     7261 gagttgacag gtcgtagacg ttcaagtaag aaaaagaaga aaaaaacatc atttttacct
     7321 ttattttatt ttatcctttt tgctttatcg attttaattt ttgtgactta ttatgtttgg
     7381 aactatattc aaactcaacc agaggagcct tctctttcta attacagtgt ggttcaatca
     7441 acaagttcaa ctagctctgt tccccactcc tcaagtagta gttcttctag tatagaatca
     7501 gctataagtg tatcaggcga aggaaatcat gtagaaatcg cttataagac aagtaaggaa
     7561 acagttaaat tgcaattggc agtttcagat gttacaagtt gggtcagtgt ttcagaaagc
     7621 gaacttgagg gcggtgtaac cttatcgcca aagaagaaaa gtgcagaagc aacagttgca
     7681 actaaaagtc ctgtaacaat tacgttaggt gttgtaaaag gtgttgattt gacagtagat
     7741 aatcagactg ttgatttatc gaaattaaca gctcagactg gacaaatcac tgtaaccttt
     7801 actaaaaatt aaggaaaaac gaatgaaaaa agaacaaatt cccaatctct taacaatagg
     7861 tcgaattctc tttataccta tttttatctt tattttaacg ataggaaatt cgatagagag
     7921 tcatatagtt gcagctatta tctttgctgt tgccagtatt accgactatt tagatggata
     7981 tttagctcgt aaatggaatg tggtcagtaa ttttggtaaa tttgcagatc ctatggcgga
     8041 taagttacta gttatgtcgg cttttattat gttgattgag ttaggtatgg ctccggcttg
     8101 gattgttgca gtgattatct gtcgtgagtt agctgtgaca ggtttaaggc ttttattggt
     8161 tgaaactggt ggaacaattt tagcagcagc aatgcctgga aaaattaaaa cttttagtca
     8221 gatgtttgct attattttct tgctattaca ttggactttg cttggtcaag ttctacttta
     8281 tgtagcctta tttttcacta tctactctgg ctatgactat ttcaagggta gtgcctatgt
     8341 atttaaaggg acatttggtt cgaaatgaaa tcaataattg atgtaaaaaa tctttctttt
     8401 cgctataaag aaaatcagaa ctactacgat gtgaaggata ttacgtttca cgtgaaacgt
     8461 ggagaatggc tttcgattgt agggcataat ggtagtggta aatcaacgac ggttcgatta
     8521 attgatggct tactggaagc agaatccgga gagattgtaa ttgatggcca acgtttgact
     8581 gaggaaaatg tttggaatat acgtcgtcaa atcggtatgg tttttcaaaa tccagacaat
     8641 caatttgttg gagcgactgt tgaagatgat gttgcttttg gtttggaaaa tcagggactt
     8701 tctcgtcaag aaatgaaaaa gagagtggaa gaagctctgg ctttagttgg catgttggac
     8761 tttaaaaaga gagagccagc gcgtctatca ggtggccaaa agcaacgtgt ggccattgca
     8821 ggtgttgtag ccctaagacc agctatttta atcttagatg aagcaacgag tatgttggat
     8881 cctgaggggc gtagagaact tattgggaca gtaaaaggaa ttcgaaaaga ctatgatatg
     8941 acggtcattt ctattaccca tgatttggaa gaagtcgcca tgagtgatcg cgtattggtc
     9001 atgaaaaaag gggaaattga atcaactagt agtccagggg agcttttctc tcgaaatgat
     9061 ttagatcaaa ttggattaga cgatcctttt gctaatcaat taaaaaaatc tttgagccag
     9121 aatggctatg atttacctga aaattatttg acagaaagtg agctagagga taagctatgg
     9181 gaattgctct agaaaatgtg aattttacat atcaagaagg tactccctta gcttcagcag
     9241 ctttgtcgga tgtttctttg acgattgaag atggctctta tacagcttta attgggcaca
     9301 caggtagtgg taaatcaact attttacaac tcttaaatgg tttattggtg ccaagtcaag
     9361 ggagtgtgag ggtttttgat accttaatca cctcgacttc taaaaataaa gatattcgtc
     9421 aaattagaaa acaggttggc ttggtatttc agtttgctga aaatcagatt tttgaagaaa
     9481 cggttttgaa ggacgttgct tttggaccgc aaaattttgg agtttctgaa gaagatgctg
     9541 tgaagactgc gcgtgagaaa ctggctctgg ttggaattga tgaatcactt tttgatcgta
     9601 gtccgtttga gctgtcaggg ggacaaatga gacgtgttgc cattgcaggc atacttgcca
     9661 tggagccagc tatattagtc ttagatgagc caacagctgg tctagatcct ctagggagaa
     9721 aagagttgat gaccctgttc aaaaaactcc accagtcagg gatgaccatc gtcttggtaa
     9781 cgcatttgat ggatgatgtt gctgaatatg caaatcaagt ctatgtaatg gaaaagggac
     9841 gtttagtaaa ggggggcaaa ccaagtgatg tctttcaaga cgttgttttt atggaagaag
     9901 ttcagttggg agtacctaaa attacggcct tttgtaaacg attggctgat agaggcgtgt
     9961 catttaaacg attaccgatt aagatagagg agtttaagga gtcgctaaat ggatagtatg
    10021 attttggggc gttatatccc aggggattcg attgttcacc gattggatcc acgtagcaaa
    10081 ttgcttgcta tgatgctact gattttgatt gtgttttggg ctaataaccc cttgacgaat
    10141 ctgattcttt ttatagcgac agggatattt attgccttgt caggagtatc tctttcattt
    10201 tttattcagg gcttgaagtc tatgtttttc ttgattgcct tcacaactat ttttcaacta
    10261 tttttcattt ctaatgggaa tgttttattt gagttttcgt ttgtgagaat cacggattat
    10321 gctttgcaac aagctgggat tattttttgt cgctttgtat tgattatttt cttttcaact
    10381 ttgttaacct taacaaccat gcctttaagt ttggcatcag ctgtcgaagc tttattagca
    10441 cctttaaagc gtgtgaaagt tccagttcat gaaattggat tgatgctgtc tatgagtttg
    10501 cgttttgtcc caaccttgat ggatgatacg acgcggatta tgaatgcaca gaaagctcgt
    10561 ggagtggatt ttggagaagg aagtatcgtt caaaaagtaa aggcgatgat tcccattttg
    10621 attcctcttt ttgcgacaag tttaaaacgt gcagattcct tggctatcgc tatggaagcg
    10681 cgtggctatc agggtggaaa aggcagaagt caataccgac aattgaaatg gactctaaag
    10741 gatacgctga ccattcttgt tattctcgta cttggttgtt gtttattttt cttaaaatct
    10801 tagtagcttt caggaattga taaaaagtta ctgtaacagt ttttgatata aaggtaggga
    10861 tatgaaccgt tttaaaaaat caaaatatgt cattattgtt tttgtcactg ttctgcttgt
    10921 gtcagctctc ttagcgacga cttattcaag tacaattgtg acaaaattag gagatggaat
    10981 ctcattggtt gatagagttg tacaaaaacc ttttcagtgg tttgattctg tcaaatcaga
    11041 tttggctcat ttgacacgaa catataatga aaatgaaagt ttgaagaaac agctttacca
    11101 attagaagtt aaatcaaatg aggtggaaag tttaaagaca gaaaatgaac aactgcgcca
    11161 attgcttgat atgaagtcta aattgcaagc cacaaagact ttagcagcag atgttattat
    11221 gcgttctccg gtatcttgga agcaggagtt gaccttagat gcaggtagat caaaaggtgc
    11281 ttctgagaac atgttagcta ttgcaaatgg tggcttgatt gggagtgttt caaaagtaga
    11341 ggagaactct actatagtca accttctgac aaatacggaa aatgctgata agatttctgt
    11401 taaaattcaa catggctcta ctacaattta tggaattatt attggctatg acaaggaaaa
    11461 tgacgttctt aaaattagcc aattaaatag taatagtgat attagtgcgg gagataaggt
    11521 gactactggt ggattaggaa actttaacgt tgctgatatt cctgttggtg aagtggttgc
    11581 cacaacgcat agtacagact atttgacacg agaagtaact gttaaattga gtgcagatac
    11641 tcataatgta gatgtgatag aattagtggg gaattcataa tgagacagtt gaagcgagtt
    11701 ggagtatttt tattgcttcc tttctttgtt ctaattgacg cccatattag ccagcttctg
    11761 ggctcatttt tcccccatgt acatttggct agtcattttc tttttctatt tctcttattt
    11821 gagacgatag aagtatcaga gtatctctac ctagtctatt gttttgttat aggcttggtt
    11881 tatgatgttt actttttcca tctaataggg attacaactc tcttatttat cttattggga
    11941 gccttccttc ataaattgaa tagtgttatt ttgttgaatc gttggacaag aatgctagct
    12001 atgattgtgc tgacattcct gtttgaaatg ggtagttatc ttttggcttt tatggtaggg
    12061 ttgacagtag atagcatgtc gatttttata gtctatagct tggtaccgac gatgatttta
    12121 aattttttat ggattactgt ttttcaattt atttttgaaa aatattatct ataagaacga
    12181 catataaatg taacaaaggc gtaatattta ttaggccttt ttttggtata ctagtattgt
    12241 ctttaaaaga aggagtatct acgtaatatg aagaaaaaaa tcttagcgtc acttttatta
    12301 agtacagtaa tggtttctca agtagctgtt ttaacaactg cgcatgcaga aacgactgat
    12361 gacaaaattg ctgctcaaga taataaaatt agtaacttaa cagcacaaca acaagaagcc
    12421 caaaaacaag ttgaccaaat tcaggagcaa gtatcagcta ttcaagctga gcagtctaac
    12481 ttgcaagctg aaaatgatag attacaagca gaatctaaga aactcgaggg tgagattaca
    12541 gaactttcta aaaacattgt ttctcgtaac caatcgttgg aaaaacaagc tcgtagtgct
    12601 caaacaaatg gagccgtaac tagctatatc aataccattg taaactcaaa atcaattaca
    12661 gaagctattt cacgtgttgc tgcaatgagt gaaatcgtat ctgcaaacaa caaaatgtta
    12721 gaacaacaaa aggcagataa aaaagctatt tctgaaaaac aagtagcaaa taatgatgct
    12781 atcaatactg taattgctaa tcaacaaaaa ttggctgatg atgctcaagc attgactacg
    12841 aaacaggcag aactaaaagc tgctgaatta agtcttgctg ctgagaaagc gacagctgaa
    12901 ggggaaaaag caagtctatt agagcaaaaa gcagcagctg aggcagaggc tcgtgcagct
    12961 gcggtagcag aagcagctta taaagaaaaa cgagctagcc aacaacaatc agtacttgct
    13021 tcagcaaaca ctaacttaac agctcaagtg caagcagtat ctgaatctgc agcagcacct
    13081 gtccgtgcaa aagttcgtcc aacatacagt acaaatgctt caagttatcc aattggagaa
    13141 tgtacatggg gagtaaaaac attggcacct tgggctggag actactgggg taatggagca
    13201 cagtgggcta caagtgcagc agcagcaggt ttccgtacag gttcaacacc tcaagttgga
    13261 gcaattgcat gttggaatga tggtggatat ggtcacgtag cggttgttac agctgttgaa
    13321 tcaacaacac gtatccaagt atcagaatca aattatgcag gtaatcgtac aattggaaat
    13381 caccgtggat ggttcaatcc aacaacaact tctgaaggtt ttgttacata tatttatgca
    13441 gattaattta cagagggact cgaatagagc cctcttttca ggttttaccg tgacaatctc
    13501 tattaaaaat tatatcaaaa tagcttgaaa atattggaaa agtatggtag aatgaaaatt
    13561 gtcgtgtgaa cgataatact cattcttgat gaattgtgaa gcagttgccc ttgggtcgtt
    13621 ttgcgagttg aagtcaagaa gaggaaaaaa acaaaaagga gaaatactca tggcagtaat
    13681 ttcaatgaaa caacttcttg aggctggtgt acactttggt caccaaactc gtcgctggaa
    13741 tcctaagatg gctaagtaca tctttactga acgtaacgga atccacgtta tcgacttgca
    13801 acaaactgta aaatacgctg accaagcata cgacttcatg cgtgatgcag cagctaacga
    13861 tgcagttgta ttgttcgttg gtactaagaa acaagcagct gatgcagttg ctgaagaagc
    13921 agtacgttca ggtcaatact tcatcaacca ccgttggttg ggtggaactc ttacaaactg
    13981 gggaacaatc caaaaacgta tcgctcgttt gaaagaaatt aaacgtatgg aagaagatgg
    14041 aactttcgaa gttcttccta agaaagaagt tgcacttctt aataaacaac gtgcgcgtct
    14101 tgaaaaattc ttgggcggta tcgaagatat gcctcgtatc ccagatgtga tgtacgtagt
    14161 tgacccacat aaagagcaaa tcgctgttaa agaagctaaa aaattgggaa tcccagttgt
    14221 agcgatggtt gacaccaata ctgatccaga tgatatcgat gtaatcatcc cagctaacga
    14281 tgacgctatc cgtgctgtta aattgatcac agctaaattg gctgacgcta ttatcgaagg
    14341 acgtcaaggt gaggatgcag tagcagttga ggcagaattt gcagcttcag aaactcaagc
    14401 agattcaatt gaagaaatcg ttgaagttgt agaaggtgac aacgcttaat ttatacaaat
    14461 agtaattacc taggagggcg gggcttagcc cggctctcct attttcaaaa aatataggag
    14521 aattaaaatg gcagaaatta cagctaaact tgtaaaagag ttgcgtgaaa aatctggtgc
    14581 cggtgttatg gacgctaaaa aagcgcttgt agaaacagac ggtgacatcg aaaaagcgat
    14641 tgaattgctt cgtgaaaaag gtatggctaa ggcagctaag aaagctgacc gtgttgctgc
    14701 agaaggtttg actggtgttt atgttaacgg taatgttgca gcagttattg aagtaaacgc
    14761 tgaaactgac ttcgttgcaa aaaacgctca attcgttgaa ttggtaaata ctacagctaa
    14821 agtcattgct gaaggaaaac ctgctaacaa cgaagaagct cttgctttga taatgccttc
    14881 aggtgaaact cttgaagctg catacgtatc tgcaacagca actataggag agaaaatctc
    14941 attccgtcgc tttgcattga ttgaaaaaac agacgcacaa cactttggag cataccaaca
    15001 taacggtgga cgtatcggtg ttatttcagt tgttgaagat ggagacgaag cacttgctaa
    15061 acaattgtca atgcacatcg cagcgatgaa accaacagtt ctttcttaca aagaattgga
    15121 tgagcaattc gttaaagatg agttggcaca attgaatcac gttatcgacc aagacaacga
    15181 aagccgtgca atggttaata aaccagctct tccacacttg aagtatggat caaaagctca
    15241 attaactgat gatgttattg ctcaagctga agctgacatc aaagctgaat tggctgcaga
    15301 aggcaaacca gaaaaaatct gggacaaaat tattccaggt aaaatggatc gcttcatgct
    15361 tgataacact aaagttgacc aagcttacac acttcttgca caagtttaca tcatggatga
    15421 cagcaagaca gttgaagcat accttgaatc agttaacgct tcggtagttg agtttgctcg
    15481 ctttgaagtt ggtgaaggta tcgagaaagc tgcaaacgac ttcgaagctg aagttgcagc
    15541 tacaatggca gcagccttga ataactaata atagaaagga agcaaattgc ttcctttttt
    15601 gtatagggct tagatttttc ctgaaattgt tttttcccca gtctcactcg attagaataa
    15661 ttaaaacaaa aagccctata ataagggcta agtgcattta ggagactata cttaaaattc
    15721 atagtaagcg cgttcataac aaggtatcta tcctaattcc ccaacttcca gtatactaga
    15781 taaaaaataa ttatcggagt aaaggtattg tactatgata attcaactaa gtgatttagg
    15841 tcaagttcac cttgtttgtg gcaagacaga tatgaggtag ggaatagact cattagccta
    15901 tgtagttaaa acccactttg aattggattc tttctccggt caagtctttc tcttttgtgg
    15961 tgaacgtaaa gaccgcttta aagcccttta ctgggatggt caaggatttt ggctactata
    16021 taaacgcttt gagaacggaa aactgacttg gctaagtaca gaaaaggatg tcaaagctct
    16081 cgcacctgaa caagtagatt ggcttatgaa gggcttttct atcactcccc aaatatagtg
    16141 gatttaaact agaatagtac acctctgctt ctaaaacatt gttagaaatc gatttgacta
    16201 tcctgttctt atttcatttt actataaatt catcagaaag tcgtgatttc tattgaaatg
    16261 aggactttct ttttatactc atctgttttc aaaaagcatt ctagtccatc tccgattaac
    16321 gatggacttt atcacctcct tctccagtcc ttgtatgaca tcttggagtt gattcatgac
    16381 atcttccaaa gttcgaaagg ccttattctt aaatccacgt ttacgaatct ctttccacac
    16441 ttgttcaatg gggttcatct ctggtgtgta tggaagaata aatgcaaagc caatattagt
    16501 cggaatcttt aaggtacttg atttatgcca tatagcattg tccataacga gtaaaagata
    16561 atcatctgga taagcttgtg aaatctccta ttcctaaagc ccctttagcg cataactttg
    16621 gctcagcttc tattatcgct cacaccatcc atcagaagtt taatctgaag gtacccaatt
    16681 atcgccaaga agaagattgg gctaggatgg gtttaccaat cacacgtaag gaaatctcta
    16741 attggcatat caagacgagt caatactatt tggagcccct ttataacctc ttgcgagaga
    16801 gactattgac tcagccctta cttcatgcgg atgaaacttc ttatagggtg ctagagagtg
    16861 atagtcagct gacttactat tggacttttt tgtctgggaa agctgagaaa caagggatta
    16921 cgctttacca tcatgtccta atcgatttgt tcatatctta tttcaatcca ttataaatag
    16981 tgagaaatat ctatcctatc ttctagaatg tcttccaaac gtggaaactc tcgtaaacaa
    17041 agaggtttta gaggcctatt taccatggat taaagttgta caagaaaagt gcaaataaga
    17101 aatctccaga ttaggaacta tccgtgagtt ctctagtctg gagatttttc aatatacttc
    17161 gttattggac ggttacaatt cataaagtgc tgtagagaga taacgttcac cgttatctgg
    17221 tgctagggca aggacttttt tacctgtacc taattttttg gcaacctcga tggctccgta
    17281 gatagctgca gctgaggaaa tccctacaag gaagccttct tttccaccaa tttcacgtcc
    17341 gagtgcaaga gcgtcatctg atgttacacg aacgatacca tcataggctt tagtatcaag
    17401 tgtatcagga ataaatccag ctgagatacc ttgaattttg tgaggaccgg gtttttcacc
    17461 agatagaata gcagattcat ctgcttctac tgcaaaaact tgaatgttag aattttctga
    17521 tttgagtgca tgagaaacac cagaaatcgt tccaccggta cctactccag caacaaaggc
    17581 atctaatcca tctttaccga aagcagctag tatctcagct cctgttgttc tttcgtgtac
    17641 ttctggatta gctggattgt caaattgaag aggaaggaaa ccatcacgtt cagcagcgat
    17701 ttcttgagcc ttagcaatag cacctttcat tccctcgcta ccaggagtta ggacgagttc
    17761 agcaccataa gcttggataa ttttacgtcg ttctacactc atagtttcag gcataacgat
    17821 gacgacttta taccctttag cagcacctac ccatgaaagt ccaataccag tgtttccact
    17881 tgttgcttca acaatagtag aaccaggttt cagaatacca tcttgttcag ctttttcaat
    17941 catgctaagg gcaatacggt cttttacaga tgaaccagga ttaaatgctt caagctttat
    18001 atagacgtct gcagcacctt ctggcacgat gttgttaagt ttaacaattg gtgtttgacc
    18061 gattaattca gtaatgttgt tataaataga catatgaata cctctttgta taagatatct
    18121 ttagtataac gctatctata ccatttgtaa aatatagaaa tcctatcgaa gcgataggat
    18181 ttctatatat catagatcaa agtcattaat tttcaagtga ttgcgttcag ctaattctag
    18241 taaataggtg tgaaggaaaa cgatatcagg tttcgtttga attgcttcat tttctagtat
    18301 attttggtac gcatcttttt attttgctcg ctaaagtagt tgtgagaatg tcaacgaaac
    18361 gttgtattat tcattgttta cactaagttt acaggaactt caacctctcg taaaccttgg
    18421 tcagttaaag tgacttttcc attaaaaaac tccacaagtg cagctttaat agtttctttt
    18481 tcttctttat caacataaat catcgtatcg atttgatctg taaagtttgt atccagctcc
    18541 atgagatcat gttctttaag gaagttactg tactcttggt actgagcata agacatttga
    18601 atagcaatgc cagcctgttc ttttatttca ataataccaa tttctttgac agctaaggcg
    18661 acactgccgg cgtaagcacg aattagtcct ccagcgccta gtttaatacc accaaagtag
    18721 cgtgtcacga ccacacagac attggtgaga ttgtgatttt ctagtacccc aagcatggga
    18781 acaccagcag taccactagg ttcaccatca tcacttgtac gtttaatttc actacgttct
    18841 ccaataataa aggcagagca gttatgtgtc gctttgtagt gttctttttt gatggcagta
    18901 atgaagtcac gagcctcttc ttcgctataa acacgcttgg catggcagat aaagcgagat
    18961 tttttgattt cttcttggac ttgaccgtcc tctttaattg ttctaaattc catgatttaa
    19021 ttgtactaaa aaatcatcta aagtgctagt ttttacgaat aaagaagtat gaaagtaaat
    19081 ttagattatc tcggtcgttt atttactgag aatgaattaa cagaagaaga acgtcagttg
    19141 gcggagaaac ttccagcaat gagaaaggag aaggggaaac ttttctgtca acgctgtaat
    19201 agtattattc tagaagaatg gtatttgccc atcggtgctt actattgtcg agagtgcttg
    19261 ctgatgaagc gagtcagaag tgatcaaact ttatactatt ttccgcagga ggattttccg
    19321 aagcaagatg ttctcaaatg gcgcggccaa ttaactcctt ttcaagagaa ggtgtcagag
    19381 ggactgcttc aagcagtaga caagcaaaag ccaaccttag ttcatgcggt aacaggagct
    19441 ggaaagacag aaatgattta tcaagtagtg gctaaagtga tcaatgcggg tggtgcagtg
    19501 tgtttggcta gtcctcgcat agatgtttgt ttggagctgt acaagcgcct gcaacaggat
    19561 ttttcttgcg gaatagcttt gctacatgga gaatcggaac cttattttcg aacaccacta
    19621 gttgttgcaa caacccatca gttattgaag ttttatcaag cttttgattt gctgatagtg
    19681 gatgaagtag atgcttttcc ttatgttgat aatcccatgc tttaccacgc tgtcaagaat
    19741 agtgtaaagg agaatggatt gagaatcttt ttaacagcga cttcgaccaa tgagttagat
    19801 aaaaaggtcc gtttaggaga actaaaaaga ctgaatttac cgagacggtt tcatggaaat
    19861 ccgttgatta ttccaaaacc aatttggtta tcggatttta atcgctactt agacaagaat
    19921 cgtttgtcac caaagttaaa gtcctatatt gagaagcaga gaaagacagc ttatccgtta
    19981 ctcatttttg cttcagaaat taagaaaggg gagcagttag cagaaatctt acaggagcaa
    20041 tttccaaatg agaaaattgg ctttgtatct tctgtaacag aggatcgatt agagcaagta
    20101 caagcttttc gagatggaga actgacaata cttatcagta cgacaatctt ggagcgtgga
    20161 gttaccttcc cttgtgtgga tgttttcgta gtagaggcca atcatcgttt gtttaccaag
    20221 tctagtttga ttcagattgg tggacgagtt ggacgaagca tggatagacc gacaggagat
    20281 ttgcttttct tccatgatgg gttaaatgct tcaatcaaga aggcgactaa ggaaattcag
    20341 atgatgaata aggaggctgg tctatgaagt gcttgttatg tgggcagact atgaagactg
    20401 ttttaacttt tagtagtctc ttacttctga ggaatgatga ctcttgtctt tgttcagact
    20461 gtgattctac ttttgaaaga attggggaag agaactgtcc aaattgtatg aaaacagagt
    20521 tgtcaacaaa gtgtcaagat tgtcaacttt ggtgtaaaga gggagttgaa gtcagtcata
    20581 gagcgatttt tacttacaat caagctatga aggatttttt cagtcggtat aagtttgatg
    20641 gagacttcct gttaagaaaa gttttcgctt catttttaag tgaggagttg aaaaagtaca
    20701 aagagtatca atttgttgta attcccctaa gtcctgatag atatgctaat agaggattta
    20761 atcaggttga gggcttggta gaggcagcag gctttgagta tctggattta ttagagaaaa
    20821 gagaagagag agccagttct tctaaaaatc gttcagagcg cttggggaca gaacttcctt
    20881 tctttattaa aagtggagtc actattccta aaaaaatcct acttatagat gatatctata
    20941 ctacaggagc aactataaat cgtgttaaga aactgttgga agaagctggt gctaaggatg
    21001 taaaaacatt ttcccttgta agatgaggaa aaaatttgca aaaatagaat gaaagcgtta
    21061 taataaaatc ataaaaacaa aaatgtttag aaagaaggta ctcatatgat taaatatagt
    21121 atccgtggtg aaaacctaga agtaacagaa gcaattcgtg attatgtagt ttctaaactc
    21181 gaaaagatcg aaaagtactt ccaaccagaa caagagttgg atgcccgaat taacttaaaa
    21241 gtttatcgtg aaaaaacggc taaagtggaa gtaacgattc cgcttggatc tattactctc
    21301 cgcgcagaag atgtatctca agatatgtat ggttcaattg accttgtaac tgataaaatt
    21361 gaacgtcaga ttcgtaaaaa taaaacaaaa atcgagcgta aaaataaaaa taaggtagca
    21421 actggtcaat tatttacaga tgctttggtg gaagattcaa atgttgtcca gtctaaagtt
    21481 gttcgttcaa aacaaattga tttaaaacca atggatttgg aagaagcaat tctacaaatg
    21541 gatttattgg ggcatgattt ctttatctat gtggatgttg aagatcagac aaccaatgtg
    21601 atttatcgtc gtgaggatgg cgaaattggt ttgttagagg ttaaagaatc ttaatttcaa
    21661 tatgtgtaaa ggtaggttta ctgaattgta aactaccttt ttcttataat tgatagaatt
    21721 actgattaca cttttaaaaa gtcgcttttt gaggggtatt atggtataat gggtgccaag
    21781 aggttttgaa tgaaaaaatt ttatgtaagt ccaatttttc ctattctagt aggattgatt
    21841 gcgtttggag tcttatccac tttcattatt tttgttaata ataatctgtt gacggtttta
    21901 attttgtttc tttttgtagg aggctatgtt tttttattta agaaactgag agtgcattat
    21961 acaaggagtg atgtagaaca gatacagtat gtaaaccacc aagcggaaga aagtttgaca
    22021 gctctattgg aacagatgcc tgtaggtgtt atgaaattga atttatcttc tggagaggtt
    22081 gagtggttta atccctatgc tgaattgatt ttgaccaagg aagatggtga ttttgattta
    22141 gaagctgttc aaacgattat caaggcttca gtaggaaatc cgtctactta tgccaagctt
    22201 ggtgagaagc gttatgctgt tcatatggat gcttcttccg gtgttttgta ttttgtagat
    22261 gtatccaggg aacaagccat aacagatgaa ttggtaacaa gtagaccagt gattgggatt
    22321 gtctctgtgg ataattatga tgatttggag gatgaaactt ctgagtcaga tattagtcaa
    22381 atcaatagtt ttgtagctaa ttttatatca gagttttcag aaaaacacat gatgttttct
    22441 cgtcgggtaa gtatggatcg attttatcta tttactgact acacggtgct tgagggcttg
    22501 atgaatgata aattttctgt tattgatgct ttcagagaag agtcgaaaca gagacagttg
    22561 cccttgacct taagtatggg attttcttat ggcgatggaa atcatgatga gatagggaaa
    22621 gttgctttgc tcaatttgaa cttggctgaa gtacgtggtg gcgaccaggt ggttgttaag
    22681 gaaaacgacg aaacgaaaaa tccagtttat tttggtggtg ggtctgctgc ttcaatcaag
    22741 cgtacacgga ctcgtacgcg cgctatgatg acagctattt cagataagat tcggagtgta
    22801 gatcaggttt ttgtagtcgg tcacaaaaat ttagacatgg atgctttggg ctctgctgta
    22861 ggtatgcagt tgttcgccag caatgtgatt gaaaatagct atgctcttta tgatgaagaa
    22921 caaatgtctc cagatattga acgagctgtt tcattcatag aaaaagaagg agttacgaag
    22981 ttgttgtctg ttaaggatgc aatggggatg gtgaccaatc gttctttgtt gattcttgta
    23041 gaccattcaa agacagcctt aacattatca aaagaatttt atgatttatt tacccaaacc
    23101 attgttattg accaccatag aagggatcag gattttccag ataatgcggt tattacttat
    23161 atcgaaagtg gtgcaagtag tgccagtgag ttggtaacgg aattgattca gttccagaat
    23221 tctaagaaaa atcgtttgag tcgtatgcaa gcaagtgttt tgatggctgg tatgatgttg
    23281 gatactaaaa atttcacctc gcgagtaact agtcggacat ttgatgttgc tagctatctc
    23341 agaacgcgcg gaagtgatag tattgctatc caggaaatcg ctgcgacaga ttttgaagaa
    23401 tatcgtgagg tcaatgaact tattttacag gggcgtaaat taggttcaga tgtactaata
    23461 gcagaggcta aggacacgaa atgctatgat acagttgtta ttagtaaggc agcagatgcc
    23521 atgttagcca tgtcaggtat tgaagcgagt tttgttcttg cgaagaatac acaaggattt
    23581 atctctatct cagctcgaag tcgtagtaaa ctgaatgtac aacggattat ggaagagtta
    23641 ggcggtggag gccactttaa tttggcagca gctcaaatta aagatgtaac cttgtcagaa
    23701 gcaggtgaaa aactgacaga aattgtatta aatgaaataa aggaaaagga gaaagaagaa
    23761 tgaaagtaat ctttttagca gatgttaaag gaaaaggtaa aaaaggcgaa attaaggaag
    23821 taccaacagg gtatgcgcaa aactttctta tcaaaaagaa tctagccaaa gaagcgactg
    23881 ctcaagctgt aggtgaactt cgaggtaaac aaaaatcgga agaaaaagct cacgctgaga
    23941 tgattgcaga aggaaaagca attaaagcac aacttgaagc agaagaaact gttgtagaat
    24001 ttgttgaaaa agttggtcca gatggtcgta cctttggttc tattaccaat aagaagattg
    24061 cagaagaatt gcaaaagcaa tttggaatta agattgataa acgtcatatt caagtacaag
    24121 ctccgattcg agcggttggt ttgattgatg tgccagtgaa aatctatcaa gatatcacaa
    24181 gtgtaatcaa tcttcgtgtg aaagaaggat aagtttacac tttcttgaca agattgtaaa
    24241 aggaagggaa gtctgatggc agaagtagaa gagttacgag tacaacctca agatatctta
    24301 gctgagcaat ccgttttagg ggctatcttt attgatgaga gtaaacttgt ttttgtgcga
    24361 gaatacattg agtctcggga cttttttaag tatgcccatc gtttgatttt ccaagccatg
    24421 gtcgatttat ccgatcgtgg tgatgccata gatgcaacaa cggttcgtac tatccttgat
    24481 aatcaaggtg atttacagaa tattggtggc ttgtcttact tggttgagat tgttaattct
    24541 gtgccaactt ctgctaatgc ggagtattat gctaagattg ttgcagaaaa agcaatgcta
    24601 cgtcgtttaa ttgccaagtt gacagagtct gtcaaccaag cttacgaagc gtcacaacca
    24661 gctgatgaaa ttattgctca ggcagaaaaa gggctgattg atgtcagtga aaatgcaaat
    24721 cgaagcgggt ttaagaacat tcgagatgtg ttgaatctca actttggaaa tctggaagct
    24781 cgctcgcaac aaacgaccga tattacaggt attgcgacag gttatcgtga tttggatcat
    24841 atgacaacag gacttcatga ggaggagttg attatcttag cagctcgtcc agcagttggt
    24901 aagacagcat ttgccttgaa tatcgctcag aacattggga ctaagttgga caaaacggtt
    24961 gctatttttt cactcgaaat gggtgcggaa agcttggtag atcgtatgtt agctgcagaa
    25021 ggcttagtgg agtcacattc tatccgtaca gggcaattga cagatgagga gtggcaaaaa
    25081 tatactattg ctcagggtaa tctagctaac gccagtatct atatcgatga tacgccaggt
    25141 attcggatta cagagattcg ttctcgttct cgtaaattgg ctcaagaaac tggaaatctt
    25201 ggtttgattt tgatagacta tttgcaactt atcacgggaa ctggtcgaga aaatcgtcaa
    25261 caagaagttt ctgaaatttc tcgtcagttg aaaatactag ccaaggaatt gaaggttcca
    25321 gtaatcgctc tgagtcagct ttctcgtggt gtagaacaac gtcaggacaa gagaccggtc
    25381 ttgtctgata ttcgtgaatc tgggtctatt gagcaggacg ctgatatcgt agcttttctc
    25441 tatcgcgatg actactatga acgtggtggt gaagaagagg agggtatccc aaataataag
    25501 gtggaagtta ttatcgagaa aaaccgtagt ggagctcgtg gaacagtgga attgattttc
    25561 caaaaagaat acaataaatt ttcaagtatc tcaaagaggg aggcataaga tgtcagatgc
    25621 atttacagat gtagccaaga tgaaaaaaat caaagaagaa atcaaggcac atgagggaca
    25681 agtcgtagaa atgactttgg agaatggtcg taagcgccaa aaaaatagat tgggtaagct
    25741 aattgaagtt tatccatctc tatttattgt ggagtttggg gatgtggaag gagataaaca
    25801 agttaatgtt tacgttgaat cctttactta ctcagatatt cttacagaaa agaatttgat
    25861 tcattatctt gactaaagtg agaaattttc tcactttttc ttttttctcc gaataattta
    25921 ggtgaaggca atcatcgctt tatattattt ttcaaggagg aagaatgaaa attttaccgt
    25981 ttatagcaag aggaacaagt tattacttga agatgtcagt taaaaagctt gttccttttt
    26041 tagtagtagg attgatgcta gcagctggtg atagtgtcta tgcctattcc agaggaaatg
    26101 gatcgattgc gcgtggggat gattatcctg cttattataa aaatgggagc caggagattg
    26161 atcagtggcg catgtattct cgtcagtgta cttcttttgt agcctttcgt ttgagtaatg
    26221 tcaatggttt tgaaattccg gcagcttatg gaaatgcgaa tgaatgggga catcgtgctc
    26281 gtcgggaagg ttatcgtgta gataatacac cgacgattgg ttccattact tggtctactg
    26341 caggaactta tggtcatgtt gcctgggtgt caaatgtaat gggagatcag attgagattg
    26401 aggaatataa ctatggttat acagaatcct ataataaacg agttataaaa gcaaacacga
    26461 tgacaggatt tattcatttt aaagatttgg atggcggcag tgttgggaat agtcaatcct
    26521 cagcttcaac aggcggaact cattatttta agaccaagtc tgctattaaa actgaacccc
    26581 tagctagcgg aactgtgatt gattactatt atcctgggga gaaggttcat tatgatcaga
    26641 tacttgaaaa agacggctat aagtggttga gttatactgc ctataatgga agctatcgtt
    26701 atgttcaatt ggaggctgtg aataaaaatc ctctaggtaa ttctgttctt tcttcaacag
    26761 gtggaactca ttattttaag accaagtctg ctatcaaaac tgaaccccta gttagcggaa
    26821 ctgtgattga ttactattat cctggggaga aggttcatta tgatcagata cttgaaaaag
    26881 acggctacaa gtggttgagt tatactgcct ataatggaag tcgtcgctat atacagctag
    26941 agggagtgac ttcttcacaa aattatcaga atcaatcagg aaatatctct agctatggat
    27001 ccaataatag ttcaactgtc ggttggaaga aaataaatgg tagttggtat catttcaaat
    27061 caaatggttc taaatcaaca ggatggctga aagacggttc tagctggtat tatttgaaat
    27121 tatctggtga aatgcagaca ggatggttaa aggaaaatgg ctcgtggtat tatctgggta
    27181 gttcagggga aatgaaaaca ggctggtacc aggtctctgg taagtggtat tattcttact
    27241 cttcaggcgc cttagctgtt aatacgacgg tggatggcta cagagtaaac agtgatggag
    27301 aacgagtata gaaaattgga gtaggagaaa tttcctgctc ttttctgtaa gcagtttcct
    27361 tgacattttt cttattttgc gctatcatat atacatataa tatatgggag tctgtgtaca
    27421 gtctgagagg aagtgttaaa cttcgaccgc acctgatctg ggtaatgcca gcggagggaa
    27481 cgatacttag tctaattttg caccttttcc atgtatggta aaggtttttc tttttttaaa
    27541 aaggaaaacg agaagaggag gttcttatga aagcaagcat tgccttgcaa gttttacccc
    27601 tagtacaggg gattgatcgg atagctgtta ttgatcaggt cattgcttat ctgcaaactc
    27661 aagaagtgcc gatggtagtg acaccatttg aaacggtctt ggaaggggag tttgatgagc
    27721 ttatgcgcat tctaaaagaa gcgctggaag tggcagggca ggaggcagac aatgtctttg
    27781 ccaatgtcaa aataaatgta ggagagattt taagtattga tgagaaactt gagaagtata
    27841 ctgagacgac acattagtct attgggcttt ctcggagtct tgtcaatctg gcagttagca
    27901 ggttttctta aactgctccc caagtttatc ctgccgacac ctcttgaaat tctccagccc
    27961 tttgttcgtg acagagaatt tctctggcac catagctggg cgaccttgag agtggcttta
    28021 ctggggctga ttttgggagt tttgattgcc tgtcttatgg ctgtactcat ggatagtttg
    28081 acttggctca atgacctgat ttaccctatg atggtggtca ttcagaccat tccgaccatt
    28141 gccatagctc ctatcctggt cttgtggcta ggttatggga ttttgcccaa gattgtcttg
    28201 attatcttaa cgacaacctt tcccatcatc gtcagcattt tggacggttt taggcattgc
    28261 gacaaggata tgctgacttt gtttagtctg atgcgggcca agccttggca aatcctgtgg
    28321 cattttaaaa ttccagtcag cctgccctac ttttatgctg gtctgagggt cagtgtctcc
    28381 tacgctttta tcacaacagt ggtatctgag tggttgggag gctttgaggg gcttggtgtt
    28441 tatatgattc agtccaagaa attgtttcag tatgatacca tgttcgctat tattattctg
    28501 gtatcgatta tcagcctttt gggtatgaag ttggtcgata ttagtgaaaa atatgttatt
    28561 aaatggaaac gttcgtagaa ttagaatgtt tctgaaaaag aaaagaggaa atcaaaatga
    28621 agaaaacatg gaaagtgttt ttaacgcttg taacagctct tgtagctgtt gtgcttgtgg
    28681 cctgtggtca aggaactgct tctaaagaca acaaagaggc agaacttaag aaggttgact
    28741 ttatcctaga ctggacacca aataccaacc acacagggct ttatgttgcc aaggaaaaag
    28801 gttatttcaa agaagctgga gtggatgttg atttgaaatt gccaccagaa gaaagttctt
    28861 ctgacttggt tatcaacgga aaggcaccat ttgcagtgta tttccaagac tacatggcta
    28921 agaaattgga aaaaggagca ggaatcactg ccgttgcagc tattgttgaa cacaatacat
    28981 caggaatcat ctctcgtaaa tctgataatg taagcagtcc aaaagacttg gttggtaaga
    29041 aatatgggac atggaatgac ccaactgaac ttgctatgtt gaaaaccttg gtagaatctc
    29101 aaggtggaga ctttgagaag gttgaaaaag taccaaataa cgactcaaac tcaatcacac
    29161 cgattgccaa tggcgtcttt gatactgctt ggatttacta cggttgggat ggtatccttg
    29221 ctaaatctca aggtgtagat gctaacttca tgtacttgaa agactatgtc aaggagtttg
    29281 actactattc accagttatc atcgcaaaca acgactatct gaaagataac aaagaagaag
    29341 ctcgcaaagt catccaagcc atcaaaaaag gctaccaata tgccatggaa catccagaag
    29401 aagctgcaga tattctcatc aagaatgcac ctgaactcaa ggaaaaacgt gactttgtca
    29461 tcgaatctca aaaatacttg tcaaaagaat acgcaagcga caaggaaaaa tggggtcaat
    29521 ttgacgcagc tcgctggaat gctttctata aatgggataa agaaaatggt atccttaaag
    29581 aagacttgac agacaaaggc ttcaccaacg aatttgtgaa ataatgacag aaattagact
    29641 agagcacgtc agttatgcct atggtcagga gaggatttta gaggatatca acctacaggt
    29701 gacttcaggc gaagtggttt ccatcctagg cccaagtggt gttggaaaga ccaccctctt
    29761 taatctaatc gctgggattt tagaagttca gtcagggaga attgtccttg atggtgaaga
    29821 aaatcccaag gggcgcgtga gttatatgtt gcaaaaggat ctgctcttgg agcacaagac
    29881 ggtgcttgga aatatcattc tgcccctctt gattcaaaag gtggataagg cagaagctat
    29941 ttcccgagcg gataaaattc ttgcgacctt ccagctgaca gctgtaagag acaagtatcc
    30001 tcatgaactt agcggtggga tgcgccagcg tgtagcctta ctccggacct acctttttgg
    30061 gcacaagctc tttctcttag atgaggcctt tagcgccttg gatgagatga caaagatgga
    30121 actccacgct tggtatcttg agattcacaa gcagttgcag ctaacaaccc tgatcatcac
    30181 gcatagtatt gaggaggccc tcaatctcag cgaccgcatc tatatcttga aaaatcgccc
    30241 tgggcagatt gtttcagaaa ttaaactaga ttggtctgaa gatgaggaca aggaagtcca
    30301 aaagattgcc tacaaacgtc aaatcttggc agaattaggc ttagataagt agaaaaatag
    30361 ggagttggtg aagattatcc tttaccagcg ccctttttct tttaaaaatg agaaaatttc
    30421 ggtataatag tcaaacaagg tcaaggttta aagagagagg tgggtttgtt atgagattta
    30481 aaaatacatc ggatcatatt gaggcctaca tcaaggcgat tttagatcaa tctggtatcg
    30541 tggagttgca acggagtcag ttggcagata cctttcaggt tgttcctagt cagattaact
    30601 acgtgatcaa gacacgcttt acggaaagta gaggctactt ggttgaaagt aagcgtggtg
    30661 gcggaggcta cattcgtata ggacggattg agttttctag tcatcatgaa atgctccggg
    30721 agctgcttta ctcgattggt gagcgagtca gtcaagaaat ttatgaggat attctccagc
    30781 ttttggttga gcaggaattg atgaccaagc aggagatgaa tttgctagaa tcagtagctt
    30841 tggatcgcgt tttaggagaa gaagctccag ttgttcgagc aaacatgcta cgtcagatca
    30901 tacaagaggt agatagaaaa gggaagtaag atgaactatt caaaagcatt gaatgaatgt
    30961 atcgaaagtg cctacatggt tgctggacat tttggagctc gttatctaga gtcttggcac
    31021 ttgttgattg ccatgtctaa tcacagttat agtgtagcag gggcaacttt aaatgattat
    31081 ccgtatgaga tggaccgttt agaagaggtg gctttggaac tgactgaaac ggactatagc
    31141 caggatgaaa cctttacgga attgccgttc tcccgtcgtt tgcaggttct ttttgacgaa
    31201 gcagagtatg tagcgtcagt ggtccatgct aaggtgctag ggacagagca cgtcttctat
    31261 gcgattttgc atgatggcaa tgccttggca actcgtatct tggagagggc tggtttttct
    31321 tatgaagaca agaaagatca ggtcaagatt gctgctcttc gtcgaaattt agaagaacgg
    31381 gcaggctgga ctcgtgaaga tctcaaggct ttacgccaac gccatcgtac agtagctgac
    31441 aagcaaaatt ctatggccaa tatgatgggc atgccgcaga ctcctagtgg tggtctcgag
    31501 gattatacgc atgatttgac agagcaagcg cgttctggca agttagaacc agtcatcggt
    31561 cgggacaagg aaatctcacg tatgattcaa atcttgagcc ggaagactaa gaacaaccct
    31621 gtcttggttg gggatgctgg tgtcgggaaa acagctctgg cgcttggtct tgcccagcgt
    31681 attgctagtg gtgacgtgcc tgcggaaatg gctaagatgc gcgtgttaga acttgatttg
    31741 atgaatgtcg ttgcagggac acgcttccgt ggtgactttg aagaacgcat gaataatatc
    31801 atcaaggata ttgaagaaga tggccaagtc atcctcttta tcgatgaact ccacaccatc
    31861 atgggttctg gtagcggaat tgattcgact ctggatgcgg ccaatatctt gaaaccagcc
    31921 ttggcgcgtg gaactttgag aacggttggt gccaccactc aggaagaata tcaaaaacat
    31981 atcgaaaaag atgcggctct ttctcgtcgt ttcgctaaag tgacgattga agaaccaagt
    32041 gtggcagata gtatgactat tttacaaggt ttgaaggcga cttatgagaa acatcaccgt
    32101 gtacaaatca cagatgaagc ggttgaaaca gcggttaaga tggctcatcg ttatttaacc
    32161 agtcgtcact tgccagactc tgctatcgac ctcttggacg aagcagcagc aacagtgcaa
    32221 aataaggcaa agcatgtaaa agcagacgat tcagatttga gtccagctga caaggccctg
    32281 atggatggca agtggaaaca ggcagcccag ctaatcgcaa aagaagagga agtacctgtc
    32341 tacaaagact tggtgacaga gtctgatatt ttgaccacct tgagtcgctt gtcaggaatc
    32401 ccagttcaaa aactgactca aacggatgct aagaagtatt taaatcttga agcagaactc
    32461 cataaacgtg tcatcggtca agatcaagct gtttcaagta ttagtcgtgc cattcgccgt
    32521 aatcagtcag gaattcgcag tcacaagcgt ccgatcggtt cctttatgtt cctaggaccc
    32581 acaggtgtcg gtaagaccga gttggccaag gctttggcag aagttctctt tgatgacgaa
    32641 tcagccctta tccgctttga tatgagtgag tatatggaga aattcgcagc cagccgtcta
    32701 aatggagctc ctccaggcta tgtgggttac gaagaaggtg gggagctgac agagaaggtt
    32761 cgcaataaac cctattccgt tctcctcttt gatgaggtag agaaggccca cccagatatc
    32821 tttaatgttc tcttgcaggt tctggatgac ggtgtcttga cagatagcaa gggacgcaag
    32881 gtcgattttt caaataccat tatcattatg acatcgaatc taggtgcgac tgcccttcgt
    32941 gatgataaga ctgttggttt tggggctaag gatattcgtt ttgaccagga aaatatggaa
    33001 aaacgcatgt ttgaagaact gaaaaaagct tatagaccgg aattcatcaa ccgtattgat
    33061 gagaaggtgg tcttccatag cctatctagt gaccatatgc aggaagtggt gaagattatg
    33121 gtcaagcctt tagtggcaag tttggctgaa aaaggcattg acttgaaatt acaagcttca
    33181 gctctgaaat tgttagcaaa tcaaggatat gacccagaga tgggagctcg cccacttcgc
    33241 agaaccctgc aaacagaagt ggaggacaag ttggcagaac ttcttcttaa gggagattta
    33301 gtggcaggca gcatacttaa gattggtgtc aaagcaggcc agttaaaatt tgatattgca
    33361 taaaagaata aaagtatcag catctgacca taagtcacag tggagtgaaa ctcaatgaaa
    33421 atcaaagagc aaactaggca gctagccgca ggttgctcaa aacactgttt tgaggttgca
    33481 gatagagctg acgtggtttg aagagatttt cgaagagtat aagctgcaag atgaatgatt
    33541 ttcttgtatt gacgttgttg ttgacaaaaa gtagcggata aatgaaatcc attccattat
    33601 catagatgat agactggtag gaaattttca aatagcatac aggaaataga tgtatggagt
    33661 tctggtagta gaaagggaga gagatgaaca ttttagttgc agatgacgag gaaatgatta
    33721 gagaaggaat tgcagcattt ctgacagaag agggttatca tgtcattatg gctaaggatg
    33781 gacaagaggt cttggaaaaa tttcaagatc tccctatcca tctcatggta ctggatttaa
    33841 tgatgcctag gaagagtggt tttgaagtgt taaaagaaat caatcaaaag cacgatattc
    33901 ctgtcatcgt cttgagtgct ctgggagatg aaactactca gtcacaggta tttgatctct
    33961 atgctgatga tcatgtgaca aaaccttttt ctttggtact gcttgtcaag cgtattaagg
    34021 cgcttatcag acgttactac gtcatagagg atatttggcg atatcaggat gtaacagtgg
    34081 attttacctc ttacaaagca cattataaaa atgaagaaat tgatctcaaa ccaaaggaat
    34141 tactggtact aaagtgtttg attcagcata aaaatcaagt tttaagtaga gagcagatat
    34201 tggaagaaat ttcaaaagat gtagctgatt taccttgtga tagggtcgtt gatgtctata
    34261 ttcgtactct tcgcaaaaaa ttagctttag attgtatcgt gactgtgaaa aatgttgggt
    34321 ataagattag tttatgataa aaaatcctaa attattaacc aagtcctttt taagaagttt
    34381 tgcaattcta ggtggtgttg gtctagtcat tcatatagct atttatttga cctttccttt
    34441 ttattatatt caactggagg gggaaaagtt taatgagagc gcaagagtgt ttacggagta
    34501 tttaaagact aagacatctg atgaaattcc aagcttactc cagtcttatt caaagtcctt
    34561 gaccatatct gctcacctta aaagagatat tgtagataag cgactccctc ttgtgcatga
    34621 cttggatatt aaagatggaa agctatcaaa ttatatcgtg atgttagata tgtctgttag
    34681 tacagcagat ggtaaacagg taaccgtgca atttgttcac ggggtggatg tctacaaaga
    34741 agcaaagaat attttgcttt tgtatctccc atatacattt ttggttacaa ttgctttttc
    34801 ctttgttttt tcttattttt atactaaacg cttgctcaat cctctttttt acatttcaga
    34861 agtgactagt aaaatgcaag atttggatga caatattcgt tttgatgaaa gtaggaaaga
    34921 tgaagttggt gaagttggaa aacagattaa tggtatgtat gagcacttgt tgaaggttat
    34981 tcatgagttg gaaagtcgta atgagcaaat tgtaaaattg caaaatcaaa aggtttcctt
    35041 tgtccgtgga gcatcacatg agttgaaaac ccctttagcc agtcttagaa ttatcctaga
    35101 gaatatgcag cataatattg gagattacaa agatcatcca aaatatattg caaagagtat
    35161 aaataagatt gaccagatga gccacttatt ggaagaagta ctggagtctt ctaaattcca
    35221 agagtggaca gagtgtcgtg agaccttgac tgttaagcca gttttagtag atattttatc
    35281 acgttatcaa gaattagctc attcaatagg tgttacaatt gaaaatcaat tgacagatgc
    35341 taccagggtc gtcatgagtc ttagggcatt ggataaggtt ttgacaaacc tgattagtaa
    35401 tgcaattaaa tattcagata aaaatgggcg tgtaatcata tctgagcaag atggctatct
    35461 ctctatcaaa aatacatgtg cgcctctaag tgaccaagaa ctagaacatt tatttgatat
    35521 attctatcat tctcaaatcg tgacagataa ggatgaaagt tccggtttgg gtctttacat
    35581 tgtgagtaat attttagaaa gctatcaaat ggattatagt tttctccctt atgaacacgg
    35641 tatggaattt aagattagct tgtagacaga ttagtttttt attaaagttc acatagggtt
    35701 aacataagtg tattattctt tatatagata aaaatataga taaaagaaag gatactaata
    35761 tggtattagc gattatttta gtaacattcc ttattcgatt gattttttta aagcgttcga
    35821 tagagaatga gaaacgaatc cttagcaatg gcgggaaaga atttggagtt gagaatacaa
    35881 aacgattaac gttggctcat attgtttttt atctctcttg cctcgttgaa gcaatggtgc
    35941 ataagacaat gtttgatggc atgggcatgg ttggtttggt cttgcttatt ttttctatgc
    36001 tgatgttgat gttggtgatt cacttgttgg gagatatttg gacagtgaag cttatgcttg
    36061 tcaataatca caaatatgta gatcatatct tgtttaggac agtaaaacac cctaattact
    36121 ttttaaatat tttacctgag ttgattggct tgaccttgtt gagtcatgcc tatatgactt
    36181 ttgttttagt ttttccagtt tatgcagtta ttttgtatcg acgaatagct gaagaggaaa
    36241 agctattaca tgaagttata atcccaaatg gaagcataaa gagataaata caaaattcga
    36301 tttatataca gttcatattg aagtgatata gtaaggttaa agaaaaaata tagaaggaaa
    36361 taaacatgtt tgcatcaaaa aacgaaagaa aagtacatta ttcaattcgt aaatttagta
    36421 ttggagtagc tagtgtagct gttgccagtc tttttatggg aagtgtggtt catgcgacag
    36481 agaaggaggt aactacccaa gtagccactt cttctaataa ggcaaataaa agtcagacag
    36541 aacatatgaa agctgctaaa caagtcgatg aatatataaa aaaaaagctc caattagata
    36601 gaagaaaaca tacccaaaat gtcggcttac tcacaaagtt gggcgtaatt aaaacggagt
    36661 atttgcatgg attaagtgtt tcaaaaaaga agtcggaagc tgagttgccg tcagaaataa
    36721 aagcaaagtt agacgcagct tttgagcagt ttaaaaaaga tacattacca acagaaccag
    36781 gaaaaaaggt agcagaagct gagaagaagg ttgaagaagc taagaaaaaa gccgaggatc
    36841 aaaaagaaaa agatctccgt aactacccaa ccaatactta caaaacgctt gaacttgaca
    36901 ttgctgagtc cgatgtggaa gttaaaaaag cggagcttga actagtaaaa gaggaagcta
    36961 aggaatctcg agacgagaaa aaaattaatc aagcaaaagc gaaagttgag aataaaaaag
    37021 ctgaggctac aaggttaaaa aacatcaaga cagatcgtga aaaagcagaa gaagctaaac
    37081 gaagagcaga tgctaagttg caggaagcta atgtagcgac ttcagagcaa gataaatcaa
    37141 agaggcgggc aaaacgagaa gttcttggag agctagcaac acctgataaa aaagaaaatg
    37201 atgcgaagtc ttcagattct agcgtaggtg aagaaactct tacaagccca tccctgaaac
    37261 cagaaaaaaa ggtagcagaa gctgagaaga aggttgaaga agctaagaaa aaagccgagg
    37321 atcaaaaaga agaagatcgt cgtaactacc caaccaatac ttacaaaacg cttgaacttg
    37381 aaattgctga gtccgatgtg gaagttaaaa aagcggagct tgaactagta aaagaggaag
    37441 ctaaggaatc tcgaaacgag gaaaaaatta agcaagtaaa agcgaaagtt gagagtaaaa
    37501 aagctgaggc tacaaggcta gaaaacatca agacagatcg taaaaaagca gaagaagaag
    37561 aagctaaacg aagagcagca gaagaagata aagttaaaga aaaaccagct gaacaaccac
    37621 aaccagcgcc ggctcctcaa ccagaaaaac caactgaaga gcctgagaat ccagctccag
    37681 ctccagctcc aaaaccagag aatccagctg aaaaaccaaa agcagaaaag ccagctgatc
    37741 aacaagctga agaagactat gctcgtagat cagaagaaga atataaccgc ttgactcaac
    37801 agcaaccgcc aaaagcagaa aaaccagcac aaccatctac tccaaaaaca ggctggaaac
    37861 aagaaaatgg tatgtggtac ttctacaata ctgatggttc aatggcgaca ggatggctcc
    37921 aaaacaatgg ctcatggtac tacctcaaca gcaatggcgc tatggcgaca ggatggctcc
    37981 aaaacaatgg ttcatggtac tatctaaacg ctaatggttc aatggcaaca ggatggctcc
    38041 aaaacaatgg ttcatggtac tacctcaacg ctaatggttc aatggcgaca ggatggctcc
    38101 aatacaatgg ttcatggtac tacct
//
LOCUS       DAVKGU010000009        37466 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKGU010000009 DAVKGU010000000
VERSION     DAVKGU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.009"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            239..808
                     /locus_tag="VW077_000437"
     CDS             239..808
                     /locus_tag="VW077_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9454516.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGKFIGTIDLHKIDPVLKKVAIGYI
                     INQTYWNQGLTTETNRAVIELAFEKIGMNKLVALYDKANPASGKVMEKSGMRFSHAEP
                     YACMYQHEKGRIVTRVHYVLTKEDYFANK"
     gene            876..1526
                     /locus_tag="VW077_000438"
     CDS             876..1526
                     /locus_tag="VW077_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU9454517.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVHK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEESVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            1510..3750
                     /locus_tag="VW077_000439"
     CDS             1510..3750
                     /locus_tag="VW077_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9454518.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GYLNTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            3929..4117
                     /locus_tag="VW077_000440"
     CDS             3929..4117
                     /locus_tag="VW077_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454519.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHGHQYAWIPSWSRYDSYSEYKVG
                     GGWNYGRYEVINYYSGGY"
     gene            4150..4737
                     /locus_tag="VW077_000441"
     CDS             4150..4737
                     /locus_tag="VW077_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454520.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            4741..5925
                     /locus_tag="VW077_000442"
     CDS             4741..5925
                     /locus_tag="VW077_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454521.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVNLNWSMGMVLLPCLIIFLLVGILFIESWGSSRKKEVFN
                     RS"
     misc_feature    6086..6217
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            6233..6763
                     /gene="infC"
                     /locus_tag="VW077_000443"
     CDS             6233..6763
                     /gene="infC"
                     /locus_tag="VW077_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9454522.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            6796..6996
                     /gene="rpmI"
                     /locus_tag="VW077_000444"
     CDS             6796..6996
                     /gene="rpmI"
                     /locus_tag="VW077_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9454523.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            7048..7407
                     /gene="rplT"
                     /locus_tag="VW077_000445"
     CDS             7048..7407
                     /gene="rplT"
                     /locus_tag="VW077_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9454524.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            7465..7845
                     /locus_tag="VW077_000446"
     CDS             7465..7845
                     /locus_tag="VW077_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9454525.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            8095..8895
                     /locus_tag="VW077_000447"
     CDS             8095..8895
                     /locus_tag="VW077_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9454526.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            8858..9844
                     /locus_tag="VW077_000448"
     CDS             8858..9844
                     /locus_tag="VW077_000448"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9454527.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            9886..12051
                     /gene="lytB"
                     /locus_tag="VW077_000449"
     CDS             9886..12051
                     /gene="lytB"
                     /locus_tag="VW077_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEU9454528.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFY
                     LKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAA
                     YYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQ
                     ALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLE
                     NPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHS
                     ALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGR
                     TFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(12112..13767)
                     /gene="pavA"
                     /locus_tag="VW077_000450"
     CDS             complement(12112..13767)
                     /gene="pavA"
                     /locus_tag="VW077_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9454529.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            13872..14369
                     /gene="ybeY"
                     /locus_tag="VW077_000451"
     CDS             13872..14369
                     /gene="ybeY"
                     /locus_tag="VW077_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9454530.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRNTDRPTDVISLEYKPELEIAFDEEDLLENSELAEMMSEFDAYIGEL
                     FISSDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            14350..14745
                     /locus_tag="VW077_000452"
     CDS             14350..14745
                     /locus_tag="VW077_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9454531.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            14762..15661
                     /gene="era"
                     /locus_tag="VW077_000453"
     CDS             14762..15661
                     /gene="era"
                     /locus_tag="VW077_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9454532.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            15710..16534
                     /gene="mutM"
                     /locus_tag="VW077_000454"
     CDS             15710..16534
                     /gene="mutM"
                     /locus_tag="VW077_000454"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9454533.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLNQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            16534..17139
                     /locus_tag="VW077_000455"
     CDS             16534..17139
                     /locus_tag="VW077_000455"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9454534.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDTQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            17126..18325
                     /locus_tag="VW077_000456"
     CDS             17126..18325
                     /locus_tag="VW077_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU9454535.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            18325..18474
                     /gene="rpmG"
                     /locus_tag="VW077_000457"
     CDS             18325..18474
                     /gene="rpmG"
                     /locus_tag="VW077_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9454536.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            18514..18747
                     /gene="secG"
                     /locus_tag="VW077_000458"
     CDS             18514..18747
                     /gene="secG"
                     /locus_tag="VW077_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9454537.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            18849..21203
                     /gene="rnr"
                     /locus_tag="VW077_000459"
     CDS             18849..21203
                     /gene="rnr"
                     /locus_tag="VW077_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9454538.1"
                     /translation="MKDRIKEYLQDKGKMTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVLSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKL
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            21166..21633
                     /gene="smpB"
                     /locus_tag="VW077_000460"
     CDS             21166..21633
                     /gene="smpB"
                     /locus_tag="VW077_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9454539.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            21649..22509
                     /gene="tehB"
                     /locus_tag="VW077_000461"
     CDS             21649..22509
                     /gene="tehB"
                     /locus_tag="VW077_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9454540.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            22582..23535
                     /gene="coiA"
                     /locus_tag="VW077_000462"
     CDS             22582..23535
                     /gene="coiA"
                     /locus_tag="VW077_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9454541.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHIANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            23554..25356
                     /gene="pepF"
                     /locus_tag="VW077_000463"
     CDS             23554..25356
                     /gene="pepF"
                     /locus_tag="VW077_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9454542.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENRGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTIFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            25358..26071
                     /locus_tag="VW077_000464"
     CDS             25358..26071
                     /locus_tag="VW077_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9454543.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFIMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            26138..27079
                     /gene="prsA"
                     /locus_tag="VW077_000465"
     CDS             26138..27079
                     /gene="prsA"
                     /locus_tag="VW077_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9454544.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(27198..27539)
                     /locus_tag="VW077_000466"
                     /pseudo
     CDS             complement(27198..27539)
                     /locus_tag="VW077_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            27951..28046
                     /locus_tag="VW077_000467"
                     /pseudo
     CDS             27951..28046
                     /locus_tag="VW077_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(28299..28919)
                     /locus_tag="VW077_000468"
     CDS             complement(28299..28919)
                     /locus_tag="VW077_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9454545.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(28919..29401)
                     /locus_tag="VW077_000469"
     CDS             complement(28919..29401)
                     /locus_tag="VW077_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9454546.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(29401..30243)
                     /locus_tag="VW077_000470"
     CDS             complement(29401..30243)
                     /locus_tag="VW077_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9454547.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(30338..31126)
                     /locus_tag="VW077_000471"
     CDS             complement(30338..31126)
                     /locus_tag="VW077_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9454548.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQALK
                     HLDKPIIDLSGWQHPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKAYKS
                     HITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMN
                     EGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKT
                     DLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            31280..32659
                     /gene="glmU"
                     /locus_tag="VW077_000472"
     CDS             31280..32659
                     /gene="glmU"
                     /locus_tag="VW077_000472"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9454549.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            32669..33214
                     /locus_tag="VW077_000473"
     CDS             32669..33214
                     /locus_tag="VW077_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9454550.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDENETLEVLEVSLEEAKELIQ
                     SGRICDAKTIMAVQYWELQKK"
     gene            33226..33540
                     /locus_tag="VW077_000474"
     CDS             33226..33540
                     /locus_tag="VW077_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9454551.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            33557..34249
                     /locus_tag="VW077_000475"
     CDS             33557..34249
                     /locus_tag="VW077_000475"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9454552.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            34326..34817
                     /gene="rocS"
                     /locus_tag="VW077_000476"
     CDS             34326..34817
                     /gene="rocS"
                     /locus_tag="VW077_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9454553.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            34915..35505
                     /locus_tag="VW077_000477"
     CDS             34915..35505
                     /locus_tag="VW077_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9454554.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQASAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            35514..37277
                     /locus_tag="VW077_000478"
     CDS             35514..37277
                     /locus_tag="VW077_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9454555.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT        11443 a         6753 c         8251 g        11019 t
ORIGIN      
        1 ggatttaccc actacaaata ttatagagcc tttttaattt cttttgggat tccgattaac
       61 attgagataa cctacctttc aattgacggt cttgttttgg ttgtcacatt ccagttcata
      121 aatcaaaaat gtgacggttt cattgtatat gaaaccgctt caaaaatcaa gaaaaacttg
      181 tcatcccaat ttttttatgc tagactagtg aaaatcaagt tctaatggag ggaaaagtat
      241 ggaatcaatt tttgtaaaac ttgcccagta tccgtctata gaaacggagc gtttattgct
      301 tagacctgta actttggatg atgcggaagc aatgtttgac tatgcctcgg acaagggtaa
      361 tacacgttac acttttccaa ccaatcaaag cttggaagaa accaagaata acattgctca
      421 gttctacttg gctaatccct tgggacgttg gggaatagaa ctaaaaagca atggcaaatt
      481 tatcggaaca attgatttgc acaagattga tcctgttctt aagaaggtag ctattggtta
      541 tattatcaac caaacgtatt ggaatcaagg attaacgaca gaaaccaatc gtgctgtgat
      601 tgagctagct tttgagaaga taggaatgaa taagttagtt gctttatacg ataaggctaa
      661 tcccgcatca ggaaaggtca tggagaaatc aggaatgcgt ttttcccatg cagaaccata
      721 tgcttgtatg taccagcatg aaaaaggccg aatcgtgaca agagttcatt atgtcttgac
      781 caaggaagac tattttgcaa ataaataagc agttgaaaag aaatttttcg actgtttttt
      841 cttcctctta cgaataatct aagagaggag aaaatatgga agcaattatc gagaaaatca
      901 aagagtataa aatcatcgtc atctgtactg gtctgggctt gcttgtagga ggatttttcc
      961 tgctaaaacc agctccacaa acacctgtca aagagacgaa tttgcaggct gaagtcgcag
     1021 ctgtttccaa ggatttggta tccgaaaagg aagtgaacaa ggaagaaaag gaagaacccc
     1081 ttgaacaaga tctaatcaca gtagatgtca aaggtgctgt caaatcgcca gggatttatg
     1141 acttgcctgt aggtagtcga gtcaatgatg ctgttcataa ggctggtggc ttgacagagc
     1201 aagcagacag caagtcgctc aatctagctc agaaagttag tgatgaggct ctggtttacg
     1261 ttcctactaa gggagaagaa tcagttagcc aacagactgg tttggggaca gcttcttcaa
     1321 taagcaagga aaagaaggtc aatctcaaca aggccagtct ggaagaactc aagcaggtca
     1381 agggactggg aggaaaacga gctcaggaca ttatcgacca tcgtgaggca aatggcaagt
     1441 tcaagtcagt agacgagctc aagaaggtct ctggcattgg tggcaaaaca atagaaaagc
     1501 ttaaagacta tgttacagtg gattaagaat ttctctattc ccctaattta cctgagtttt
     1561 ctattacttt ggctttatta cgctattttc tcagcatctt atcttgcttt gttgggcttt
     1621 gtttttctgc tagtctgtct ctttatccaa tttccgtgga aatctgctgg taaagttcta
     1681 ataatttgcg gaatctttgg attttggttt gtttttcaaa attggcaaca gagtcaagtg
     1741 agtcaaaatt tggtggattc tgttgaaagg gtacggattt taccagacac tattaaggtt
     1801 aacggtgata gtctatcctt tcgtggcaag tctaacggtc gtgctttcca agtctattat
     1861 aaactccagt ccgaggagga gaaagaagcc tttcaagctt taaccgacct gcatgagata
     1921 ggactagaag ggaagctttc ggagccagaa gggcagagaa attttggtgg ctttaattac
     1981 caagcctatc tgaagactca gggaatttac cagactctca atatcaaaaa aatccagtca
     2041 cttcaaaaga ttggcagttg ggatatagga gaaaacttgt ccagtttacg tcgaaaggct
     2101 gtggtttgga ttaagacgca ctttccagac cctatgcgca attacatgac aggactcttg
     2161 ctgggatatc tgaacaccga ctttgaggag atgaatgagc tttattccag tctaggaatt
     2221 atccacctct ttgccctatc tggcatgcag gtaggttttt tcatgaatgg atttaagaaa
     2281 cttctcttgc gattgggctt gacccaagaa aagttgaaat ggctgactta tcccttttcc
     2341 cttatctatg cgggactaac tggattttca gcatcggtta ttcgcagtct cttgcaaaag
     2401 ctactggctc aacatggggt taagggcttg gataattttg ccttgacggt gcttgtcctc
     2461 tttattgtca tgccaaactt tttcttgaca gcaggaggag tcttgtcctg cgcttatgct
     2521 tttatcctga ccatgaccag caaagaaggg gaggggctca aggctgttgc tagtgaaagt
     2581 ctagtcatct ccttgggcat attgcccatt ctatccttct attttgcgga atttcaacct
     2641 tggtctatcc ttttgacctt tgtcttttcc tttctatttg acttgacctt cttaccgctc
     2701 ttgtctattt tatttgtcct ttcctttctc tatccagtca ttcagctgaa ctttatcttt
     2761 gaatggttag agggcattat tcgcttggtc tcgcaggtgg caaggagacc gcttgtcttt
     2821 ggtcaaccca acgcatggct tttaatctta ttgttaattt ccttggcttt ggtctatgat
     2881 ttgaggaaaa acattaaagg attaacagta ttgagtttat tgattacagg tctctttttc
     2941 cttaccaagt atccactgga aaatgaaatc accatgctgg atgtggggca aggagaaagt
     3001 attttcctac gggatgtaac tgggaaaacc attctcatag atgtaggtgg taaggcagaa
     3061 tcttataaga aaatcgaaaa atggcaagaa aagatgacga ccagcaatgc acagagaagc
     3121 ttgataccct atcttaaaag tcgaggagta gctaagattg accagctgat tttgacaaat
     3181 acggacaagg aacatgtcgg agatttgtta gaggtgacca aggctttcca tgtaggggag
     3241 attttagtat caaaaggcag tttgaagcag aagcaatttg tggtagaact acaggcgact
     3301 caaacaaagg tgcgtagtat gacagtaggg gagaacttgc ccatttttgg aagtcagtta
     3361 gaagttctat ccccaaggaa aatgggagat ggaggacacg atgataccct agttctgtat
     3421 gggaaattct tggataagca atttctcttc acgggaaatt tggaggagaa aggagagaag
     3481 gacttgctga agcactatcc agacttgaaa gtaaatgttt tgaaagctag ccaacatggc
     3541 aataaaaaat catcaagtcc agcctttcta gaaaaactca aaccagagct tactcttatc
     3601 tcagttggaa agagcaatcg aatgaaactc ccccatcagg aaacattgac acgactggaa
     3661 ggtatcaata gcaaagttta ccgaactgac cagcaaggag ctatacgctt taaggggttg
     3721 gatagttgga aaatcgaaag tgttcgatag gaaagataaa tgttgtagat tagtgaaata
     3781 aactaaaaat ttgttgcata ataatgataa aaatggtata atgaaaacgt attcaatatt
     3841 gaggatataa aatcattaaa aattagcaaa aatcgtttaa ttagttagtt tataatctat
     3901 tggtcttctt cagtccagtg tatctgctgt gacagtcact aaaagttaca agtatgattg
     3961 gaatacggtt tgggaatata gtaccaacta tcacggccat cagtatgctt ggattccgtc
     4021 atggtctcgt tatgacagct attctgagta taaagttggc ggaggctgga actacggtcg
     4081 ttatgaggtc ataaactatt acagcggagg ctattaattc ttaaagagtg agaaaaagga
     4141 gggctagata tgttgcagct tactcatgtg accttaaaaa cgcgacaagt catcttgcaa
     4201 gatgcggatt ttacctttaa aaagggtagg atttatggcc ttcttgctat caatggctcg
     4261 ggaaagacga cactattccg agctatgagc aagttgcttc cccttagtag tggacacatc
     4321 gcagttcctc cttctttgtt ttattatgag agcgttgaat ggctggatgg aaacttaagt
     4381 gggatggact accttcgtct cataaaaaac atctggaagt cagacctaaa cttgagagat
     4441 gaaatcgcct actgggaaat ggctgactat atcagtcttc ccatccgcaa gtattcctta
     4501 ggcatgaagc aacgcttggt gattgctatg tattttctca gtcaggccaa atgctggctc
     4561 atggatgaga ttacaaatgg cttagacgag tattatcgac agaagttttt tgataggcta
     4621 gcacaaatcg atagacaaga acagctggtt cttttaagtt cccactataa ggaagagttg
     4681 gttgatatct gcgatagagt agtaaccatt catcaggggc agatagaaga ggtttagttt
     4741 atgaaagatg ttagtctatt tttattgaaa aaagttttca aaagtcgttt aaactggatt
     4801 atcttacttt tatttgcatc tgtactcggt gttacctttt atttaaatag tcagactgca
     4861 aactcacaca gcttggagag caggttggaa agtcgcattg cagccaacga gagggctatc
     4921 aatgaaaatg aagagaaact ctcccaaatg tctgatacca gctcggagga ataccagttt
     4981 gctaaaaata atttagacgt gcaaaaaaat cttttgacgc gaaagacaga aattctaact
     5041 ttactaaaag aagggcgctg gaaagaagcc tactatctgc agtggcaaga tgaagagaag
     5101 aattatgaat ttgtatcaaa tgacccgact gctagctctg gcttaaaaat gggggttgac
     5161 cgcgaacgga agatttacca agccctgtat cccttgaaca taaaagcaca tactttggag
     5221 tttccgaccc acgggattga tcagattgtc tggattttag aggttatcat cccaagcttg
     5281 tttgtggttg cgattatttt tatgctaaca caactatttg cagaaagata tcaaaatcat
     5341 ctggacacag ctcacttata tcctgtttca aaagtgacat ttgcaatatc ctctcttgga
     5401 gttggagtgg gatatgtaac tgtgctgttt atcggaatct gtggcttttc ttttctagtg
     5461 ggaagtctga taagtggttt tggacagtta gattatccct acccaattta tagcttagtg
     5521 aatcaagaag taactattgg gaaaatacaa gatgtattat ttcctggctt gctcttagct
     5581 ttcttagcct ttatcgtcat tgtggaagtt gtgtacttga ttgcttactt tttcaagcaa
     5641 aaaatgcctg tcctctttct ttcactcatt gggattgttg gcttattgtt tggcatccaa
     5701 accattcagc ctcttcaaag gattgcacat ctgattccct ttacttactt gcgttcagtg
     5761 gagattttat ctggaagatt acctaagcaa attgataatg tcaatctaaa ttggagcatg
     5821 ggaatggtct tacttccttg cctgattatc tttttgctag tggggattct atttattgaa
     5881 agctggggaa gttcacgtaa aaaggaagtt tttaataggt cttagcattc ttataaggaa
     5941 gttaaggaaa aactaacata gagagggaat caacttgatt ctctcttttt gattcgaaaa
     6001 ccaaaccaaa atacaaacac aaacttttca aaaaaataac tttttatctt gacaagagct
     6061 agaaaacttg gtatcattta aaagttgaga aaagcagaag tgagagcttc tcgccttgtg
     6121 acattaagtt gcctggccct acggatgaaa agtttcgaag aaacgctatc ataacgtgcg
     6181 ggcttgtata tttacgagtc cgctattgtt tttctctaat aaaacaaaag aggtgaaaac
     6241 catagcaaag caagacttat tcatcaatga tgagattcgt gtacgtgaag ttcgcttgat
     6301 tggtcttgag ggagaacagc taggcatcaa gccactcagt gaagcgcaag ctttggctga
     6361 taacgctaat gttgacctag tattgattca accccaagcc aaaccgcctg ttgcaaaaat
     6421 tatggactac ggtaagttca aatttgagta ccagaagaag caaaaagaac aacgtaaaaa
     6481 acaaagcgtt gttactgtga aagaagttcg tctaagtcca acaattgaca agggtgactt
     6541 tgatacaaaa cttcgtaatg cacgtaagtt ccttgaaaaa ggaaataaag ttaaggtatc
     6601 tattcgcttt aagggtcgta tgattaccca taaagagatt ggtgcaaaag ttttagccga
     6661 gtttgctgaa gcaactcaag atattgccat catcgaacaa cgtgctaaaa tggatggacg
     6721 tcaaatgttc atgcaattgg cgccagcgac tgacaaaaaa taattgtcag aaagttaaat
     6781 aaaggagaaa aaatcatgcc aaaacaaaaa acacaccgcg catcagctaa acgtttcaaa
     6841 cgtacaggtt ctggtggact taaacgtttc cgtgcttaca cttctcaccg tttccacgga
     6901 aaaactaaga aacaacgtcg tcatcttcgt aaagcatcta tggtgcattc aggagattac
     6961 aaacgtatca aagcaatgct tactcgcttg aaataatagc ttgactgtaa gtaaacaatt
     7021 ctaggaaata tttggaggaa ataaaacatg gcacgtgtta aaggtggcgt tgtatcacgc
     7081 aaacgtcgta aacgtattct taaattagca aaaggttact atggagctaa acacatcttg
     7141 ttccgtactg caaaagaaca agtaatgaac tcttactact atgcataccg tgaccgtcgt
     7201 cagaaaaaac gtgacttccg caaattgtgg attactcgta tcaatgcggc agctcgtatg
     7261 aacggacttt catactcaca attgatgcat ggtttgaaat tggctgagat cgaagttaac
     7321 cgtaaaatgc ttgctgactt ggctgttaac gatgcagtag ctttcacagc tcttgcagat
     7381 gcagctaaag caaaacttgg taaataacaa ctgatgagac tggaacagga ttgtccagtc
     7441 tttttaaaaa taaaggagaa agatatggct tcaaaaatgc tacacacttg cttacgagta
     7501 gaaaatcttg aaaaatcaat cgcattttat caagatgctt ttggttttaa agaattgcgt
     7561 cgcagagatt ttccagacca tgccttcacg attgtctatc taggtcttga gggtgatgat
     7621 tatgagttgg agttgactta taactacgat cacggtccgt atgtggttgg tgatgggttt
     7681 gcccatatcg ccctcagtac acctgatctt gaggcacttc atcaagaaca cagtgcaaaa
     7741 ggctatgaag tgactgagcc aaatggtcta ccaggaacta caccaaacta ttactttgtc
     7801 aaagaccctg atggatataa ggtcgaagtc attcgtgaaa aataatttcg tgcaagttaa
     7861 gtagaatatt cgttttctac ttgacttttt ttagctgaaa gagtatacta ataaaaattc
     7921 aacctttaag gggagtccag agagactcac aaggtgtcag ataaaagaat agtgcaattt
     7981 tctagaggag actttttgag tgtgctctct tgtgttgtac gattttaact gaggccttgc
     8041 actagcaagg tcttttcttt atctggtccc cttaaaattt aaggaggaaa agttatgaat
     8101 cccacatgta agaagcgttt gggtgtcatt cggttggaaa ccatgaaggt ggttgcacaa
     8161 gaggaaatcg cgccagcaat ctttgaatta gtcctagaag gagaaatggt tgaagccatg
     8221 cgagcaggcc aatttcttca tctgcgtgta ccagacgatg cccatctttt acgtcgtcct
     8281 atttcaattt cgtctattga caaggcaaac aagcagtgtc acctcattta tcggattgag
     8341 ggagctggga ctgcaatttt ttcaacctta agtcagggag acactcttga tgtgatgggg
     8401 cctcagggaa atggttttga cttgtctgac cttgatgagc agaatcaggt tctccttgtt
     8461 ggtggtggga ttggtgttcc acccttgctt gaggtggcca aggaattgca tgaacgtgga
     8521 gtgaaagtag tgacagtcct cggttttgct aataaggatg ctgttatttt gaaaacggaa
     8581 ttggctcagt atggtcaggt ctttgtaacg acagatgatg gttcttatgg catcaaggga
     8641 aatgtttccg ttgttatcaa tgatttagat agtcagtttg atgctgttta ctcgtgtggg
     8701 gctccaggaa tgatgaagta tatcaatcaa acctttgatg atcacccaag agcctattta
     8761 tctctggaat ctcgtatggc ttgtgggatg ggagcttgct atgcctgtgt tctaaaagta
     8821 ccagaaagcg agacggtcag ccaacgcgtc tgtgaagatg gtcctgtttt ccgcacagga
     8881 acagttgtat tataaggaga aaattatgac tacaaatcga ttacaagtgt ctctacctgg
     8941 tttggatttg aaaaatccga ttattccagc atcaggttgt tttggttttg gacaagagta
     9001 tgccaagtac tatgatttaa accttttagg ttctattatg atcaaggcga caacccttga
     9061 accacgtttt ggaaatccaa ctccaagagt ggcagagacg cctgctggta tgctcaatgc
     9121 aattggcttg caaaatcctg gtttagaggt tgttttggct gaaaagctac cttggctgga
     9181 aagagaatat ccaaatcttc ctattattgc caatgtagct ggtttttcaa aacaagagta
     9241 tgcagctgtt tctcatggga tttccaaggc aactaatgta aaagctatcg agctcaatat
     9301 ttcttgtccc aatgttgacc actgtaatca tggacttttg attggtcaag atccagattt
     9361 ggcttatgat gtggtgaaag cagctgtgga agcctcagaa gtgccagttt atgtcaaatt
     9421 aaccccgagt gtgaccgata tcgttactgt cgcaaaagct gcagaagatg cgggagcaag
     9481 tggcttgacc atgatcaata ctctggttgg aatgcgcttt gacctcaaaa ccagaaaacc
     9541 aatcttggcc aatggaacag gtggaatgtc aggtccagca gtctttccag tagccctcaa
     9601 actcatccgc caagtagccc aaacaacaga cctgcctatc attggaatgg ggggagtgga
     9661 ttcggctgaa gctgccctag aaatgtatct ggctggggca tctgctatcg gagttggaac
     9721 agctaacttt accaatcctt atgcctgccc tgacatcatc gaaaatttac caaaagtcat
     9781 ggataaatac ggtattagca gtctggaaga actccgtcag gaagtaaaag agtctctgag
     9841 gtaaactaca atcaatctgt tcttgatttt ttattagttt gtaatatgaa tttaggagaa
     9901 ttttggtaca ataaaataaa taagaacaga ggaagaaggt taatgaagaa agtaagattt
     9961 atttttttag ctctgctatt tttcttagct agtccagagg gtgcaatggc tagtgatggt
    10021 acttggcaag gaaaacagta tctgaaagaa gatggcagtc aagcagcgaa tgagtgggtt
    10081 tttgatactc attatcaatc ttggttctat ataaaagcag atgctaacta tgctgaaaat
    10141 gaatggctaa agcaaggtga cgactatttt tacctcaaat ctggtggcta tatggccaaa
    10201 tcagaatggg tagaagacaa gggagccttt tattatcttg accaagatgg aaagatgaaa
    10261 agaaatgctt gggtaggaac ttcctatgtt ggtgcaacag gtgccaaagt aatagaagac
    10321 tgggtctatg attctcaata cgatgcttgg ttttatatca aagcagatgg acagcacgca
    10381 gagaaagaat ggctccaaat taaagggaag gactattatt tcaaatccgg tggttatcta
    10441 ctgacaagtc agtggattaa tcaagcttat gtgaatgcta gtggtgccaa agtacagcaa
    10501 ggttggcttt ttgacaaaca ataccaatct tggttttaca tcaaagaaaa tggaaactat
    10561 gctgataaag aatggatttt cgagaatggt cactattatt atctaaaatc cggtggctac
    10621 atggcagcca atgaatggat ttgggataag gaatcttggt tttatctcaa atttgatggg
    10681 aaaatagctg aaaaagaatg ggtctacgat tctcatagtc aagcttggta ctacttcaaa
    10741 tccggtggct acatggcagc caatgaatgg atttgggata aggaatcttg gttttacctc
    10801 aagtttgatg gcaaaatggc tgaaaaagaa tgggtctacg attctcatag tcaagcctgg
    10861 tactacttca aatccggtgg ttacatgaca gccaatgaat ggatttggga taaggaatct
    10921 tggttttatc tcaaatctga tgggaaaata gctgaaaaag aatgggtcta cgattctcat
    10981 agtcaagctt ggtactactt caaatccggt ggttacatga cagccaatga atggatttgg
    11041 gataaggaat cttggtttta cctcaagtct gatggcaaaa tggctgaaaa agaatgggtc
    11101 tacgattctc atagtcaagc ctggtactac ttcaaatctg gtggctacat ggcgaaaaat
    11161 gagacagtag atggttatca gcttggaagc gatggtaaat ggcttggagg aaaagctaca
    11221 aatgaaaatg ctgcttacta tcaagtagtg cctgttacag ccaatgttta tgattcagat
    11281 ggtgaaaagc tttcctatat atcgcaaggc agtgtcgtat ggctagataa ggatagaaaa
    11341 agtgatgaca agcgcttggc tattactatt tctggtttgt caggctatat gaaaacagaa
    11401 gatttacaag cgctagatgc tagtaaggac tttatccctt attatgagag tgatggccac
    11461 cgtttttatc actatgtggc tcagaatgct agtatcccag tagcttctca tctttctgat
    11521 atggaagtag gcaagaaata ttattcggca gatggcctgc attttgatgg ttttaagctt
    11581 gagaatccct tccttttcaa agatttaaca gaggctacaa actacagtgc tgaagaattg
    11641 gataaggtat ttagtttgct aaacattaac aatagccttt tggagaacaa gggcgctact
    11701 tttaaggaag ccgaagaaca ttaccatatc aatgctcttt atctccttgc ccatagtgcc
    11761 ctagaaagta actggggaag aagtaaaatt gccaaagata agaataattt ctttggcatt
    11821 acagcctatg atacgacccc ttacctttct gctaagacat ttgatgatgt ggataaggga
    11881 attttaggtg caaccaagtg gattaaggaa aattatatcg ataggggaag aactttcctt
    11941 ggaaacaagg cttctggtat gaatgtggaa tatgcttcag acccttattg gggcgaaaaa
    12001 attgctagtg tgatgatgaa aatcaatgag aagctaggtg gcaaagatta gtactataag
    12061 tgaatatgat ttgagtgaat agtaagttaa aaatcctgat ttcaagtaaa atcaggattt
    12121 tttcatggat gcaatttttt tggagtctgg tgtgacgcgg agggtctttt gtcctgtgta
    12181 agtgacaaag ccgggttttc caccagttgg tttattgagt tttttgactt caatcatatc
    12241 tacctgcacc agattcgaca ggcgcccttg agagaagtag gcagctaact ctgctgcgtc
    12301 tgtcttgact gcatcagatg ggtcaagatt tcctgagatg acaacatggc ttccaggaat
    12361 gtccttagca tggaaccaaa gttcctcctt gcgggccatt ttaaaggtca attcctcatt
    12421 ttgaagattg tttcgtccga catagatgat ggttttgcca tcgcttgcta gatattgttc
    12481 tagttttttg cgtttctgga ttttctcccg ttgtcttctg cggataaaac ctgtttgaat
    12541 caattcttca cggatttcag cgatttcttc cagtccagct tggttgagga cggtttctac
    12601 actttccaga tagagaatag tggctttggt ttcttcaatc aaatcagtca agtatttgac
    12661 agcttctttg agtttctgat accgtttaaa atagcgttgg gcattctggt tgggagtcag
    12721 agccttatca agcgcaatca tgataggttg gttggtatag tagttgtcta ggataacctg
    12781 gtcttggtcg ttaggcactt ggtgtaggaa ggttgtcagc aattctcctt tttgacgaaa
    12841 ttcttcagcg ttgtctgtcg ccagtaactc tttttcctgt tttttgagtt tgtgtcggtt
    12901 tttctgaagt tcattttcaa cacgacgaat cagttcactg gcctgctgtt tgacgcggtc
    12961 gcgctcagcc ttatccttat agtaggtgtc caacaaatca gaaagatttg caaaaggctc
    13021 tcccacctga tttgcaaaag gaactggact gaaggaagtc tcagtcaagc atggcttggt
    13081 ttcttgattg aaaaaatttc ggaaagcgga aagtttttca ctaaccagta tcctttccaa
    13141 ttcatttgcc gtatcgcgtc ccagaccttg aaagaggctt tgaagatttt ttgctgttag
    13201 ttcttgggtt tgcaggattt caaagagctt ttcatccttg atagtaaaag gattgagaga
    13261 ttctgtactt ggcggagcga tataggtcga tcctggaagt aaggtgcggt agctattttg
    13321 tgaaaaaccg acgtgtttga taacttcgag gattttatga ctgcttttat cgaccagtag
    13381 aatattactg tgtttcccca taatttcgat aatcaaggta gcctggatat ggtctccaat
    13441 ctcgttttta ttggaaactg taatttccac aatacggtca ttttccactt gctcaatcga
    13501 ctcaatcagg gccccctgca aatactttct caaaaccatg ataaaggtag aaggttgagc
    13561 tggattttca aaagtcgttt gggtcagctg aatgcgtcca aaaactggat gggcagaaag
    13621 gagcaggcga tggctttggc gattgctgcg gatttgcaag accaactctt gttcaaaagg
    13681 ctgattgatt ttctggatgc gaccattcac taattcgctt cgcaattcct caactatgtg
    13741 gtgtaaaaaa aatccgtcaa atgacatcgt tctctccttg tgattgtatt ccatagtatt
    13801 atatcaaaaa ggtagaataa aatcatggaa atgtggtata ataaagccaa gtaaagagaa
    13861 acgagaagca catgtatatt gaaatggtag atgaaactgg tcaagtttca aaagaaatgt
    13921 tgcaacaaac ccaagaaatt ttggaatttg cagcccaaaa attaggaaaa gaagacaagg
    13981 agatggcagt cacttttgtg accaatgagc gtagtcatga acttaatctg gagtaccgta
    14041 acaccgaccg tccgacagat gtcatcagcc ttgagtataa accagaattg gaaattgcct
    14101 ttgacgaaga ggatttgctt gaaaattcag aattggcaga gatgatgtct gagtttgatg
    14161 cctatattgg ggaattgttc atctctagcg ataaggctca tgagcaggcc gaagaatatg
    14221 gtcacagctt tgagcgtgag atgggcttct tggcagtaca cggcttttta catattaacg
    14281 gctatgatca ctacactccg gaagaagaag cggagatgtt cggtttacaa gaagaaattt
    14341 tgacagccta tggactcaca agacaataaa cgaaaatgga aaaatcgtga cctgatatcc
    14401 agtttagaat ttgccatcac agggattttt actgctatca aggaagaacg aaatatgcga
    14461 aaacatgcag tgacggctct agtggtcatc cttgcaggtt ttgtttttca ggtgtcacga
    14521 atcgaatggc tctttctcct attgagcatt ttcttggtag tagcctttga gattatcaac
    14581 tctgctattg aaaatatggt ggatttggcc agtcactatc acttttccat gctggctaaa
    14641 aatgccaagg atatggcggc tggcgcggta ttagtggttt ctcttttcgc agccttaaca
    14701 ggtgcattga tttttctccc acgaatctgg gatttattat tttaaacagt aagaggaaat
    14761 tatgactttt aaatcaggct ttgtagccat tttaggacgt cccaatgttg ggaagtcaac
    14821 ctttttaaat cacgttatgg ggcaaaagat tgccatcatg agtgacaagg cgcagacaac
    14881 gcgcaataaa atcatgggaa tttacacgac tgataaggag caaattgtct ttatcgacac
    14941 accagggatt cacaaaccta aaacagctct cggagatttc atggttgagt ctgcctacag
    15001 tacccttcgc gaagtggaca ccgttctttt catggtgcct gctgatgaag cgcgtggtaa
    15061 gggggacgat atgattatcg agcgtctcaa ggctgccaag gttcctgtga ttttggtggt
    15121 gaataaaatc gataaggtcc atccagacca gctcttgtct cagattgatg acttccgtaa
    15181 tcaaatggac tttaaggaaa ttgttccaat ctcagccctt cagggaaata acgtgtctcg
    15241 tctagtggat attttgagtg aaaatctgga tgaaggtttc caatatttcc cgtctgatca
    15301 aatcacagat catccagaac gtttcttagt ttcagaaatg gttcgcgaga aagtcttgca
    15361 cctaactcgt gaagagattc cgcattctgt agcagtagtt gttgactcta tgaaacgaga
    15421 cgaagagaca gacaaggttc acatccgtgc aaccatcatg gtcgagcgcg atagccaaaa
    15481 agggattatc atcggtaaag gtggcgctat gcttaagaaa atcggtagta tggcccgtcg
    15541 tgatatcgaa ctcatgctag gagacaaggt cttcctagaa acctgggtca aggtcaagaa
    15601 aaactggcgc gataaaaagc tagatttggc tgactttggc tataatgaaa gagaatacta
    15661 agtagaggta ggctcatgcc tgcttcttgt ttttacagaa ggaggactta tgcctgaatt
    15721 acctgaggtt gaaaccgttt gtcgtggctt agaaaaattg attataggaa agaagatttc
    15781 gagtatagaa attcgctacc ccaagatgat taagacggat ttggaagagt ttcaaaggga
    15841 attgcctagt cagattatcg agtcaatggg acgtcgtgga aaatatttgc ttttttatct
    15901 gacagacaag gtcttgattt cccatttgcg gatggagggc aagtattttt actatccaga
    15961 ccaaggacct gaacgcaagc atgcccatgt tttctttcat tttgaagatg gtggcacgct
    16021 tgtttatgag gatgttcgca agtttggaac catggaactc ttggtgcctg accttttaga
    16081 cgcctacttt atttctaaaa aattaggtcc tgaaccaagc gaacaagact ttgatttaca
    16141 ggtctttcaa tctgcccttg ccaagtccaa aaagcctatc aaatcccatc tcctagacca
    16201 gaccttggta gctggacttg gcaatatcta tgtggatgag gttctctggc gagctcaggt
    16261 tcatccagct agatcttccc agactttgac agcagaagaa gcgacagcta ttcatgacca
    16321 aaccattgct gttttgaacc aggctgttga aaaaggcggt tccaccattc ggacctatac
    16381 caatgccttt ggggaagatg gaagcatgca ggattttcat caggtctatg ataagactgg
    16441 tcaagaatgt gtacgctgtg gtaccatcat tgagaaaatt caactaggcg gacgaggcac
    16501 ccacttttgt ccaaactgtc aaaggaggga ctgatgagaa aaatcatcgg aatcactggg
    16561 ggaattgcct ctggtaagtc aactgtgaca aattttctaa gacagcaagg ctttcaagca
    16621 gtggatgccg acgcagtcgt ccaccaacta cagaaacctg gtggtcgtct gtttgaggct
    16681 ctagtacagc actttgggca agaaatcatt cttgaaaacg gagaactcaa tcgccctctc
    16741 ctagctagtc tcatcttttc aaatcctgaa gagcaaaaat ggtctaagca aattcaaggg
    16801 gagattatcc gtgaggaact ggctactttg agagaacagt tggctcagac agaagagatt
    16861 ttcttcatgg atattcccct actttttgag caggactaca gcgattggtt tgctgagact
    16921 tggttggtct atgtggaccg agatacccaa gtagaacgct taatgaaaag ggaccagttg
    16981 tccaaggatg aagctgagtc tcgtctggca gcccagtggc ctttagaaaa aaagaaagat
    17041 ttggccagcc aggttcttga taataatggc aatcagaacc agcttcttaa tcaagtgcat
    17101 atccttcttg agggaggtag gcaagatgac agagattaac tggaaggata atctgcgcat
    17161 tgcctggttt ggtaattttc tgacaggagc cagtatttct ttggttgtac cttttatgcc
    17221 catcttcgtg gaaaatctag gtgtagggag tgagcaagtc gctttttatg caggcttagc
    17281 aatttctgtc tctgctattt ccgcggcgct cttttctcct atttggggta ttcttgctga
    17341 caaatacggc cgaaaaccca tgatgattcg ggcaggtctt gctatgacta tcactatggg
    17401 aggcttggcc tttgtcccaa atatctattg gttaatcttt cttcgtttac taaacggtgt
    17461 atttgcaggt tttgttccta atgcaacggc actgatagcc agtcaggttc caaaggagaa
    17521 atcaggctct gccttaggta ctttgtctac aggcgtagtt gcaggtactc taactggtcc
    17581 ctttattggt ggctttatcg cagaattatt tggcattcgt acagttttct tactggttgg
    17641 tagttttcta tttttagctg ctattttgac tatttgcttt atcaaggaag attttcaacc
    17701 agtagccaag gaaaaggcta ttccaacaaa ggaattattt acctcggtta aatatcccta
    17761 tcttttgctc aatctctttt taaccagttt tgtcatccaa ttttcagctc aatcgattgg
    17821 ccctattttg gctctttatg tacgcgactt agggcagaca gagaatcttc tttttgtctc
    17881 tggtttgatt gtgtccagta tgggcttttc cagcatgatg agtgcaggag tcatgggcaa
    17941 gctaggtgac aaggtgggca atcatcgtct cttggttgtc gcccagtttt attcagtcat
    18001 catctatctc ctctgtgcca atgcctctag cccccttcaa ctaggactct atcgtttcct
    18061 ctttggattg ggaaccggtg ccttgattcc cggggttaat gccctactca gcaaaatgac
    18121 tcccaaagcc ggcatttcga gggtctttgc cttcaatcag gtattctttt atctgggagg
    18181 tgttgttggt cccatggcag gttctgcagt agcaggtcaa tttggctacc atgctgtctt
    18241 ttatgcgacg agcctttgtg ttgcctttag ttgtctcttt aacctgattc aatttcgaac
    18301 attattaaaa gtaaaggaaa tctagtgcga gtaaaaatta atctcaaatg ctcctcttgt
    18361 gacagtatca attacctaac cagtaaaaat tcaaaaaccc atccagacaa gattgaggtt
    18421 ttaaagtatt gtcccaagga aagaaaagta accctacatc ttgaatctaa gtagatttta
    18481 tggtaaaata atagggattt taaggagttt gatatgtata acctattatt aaccatttta
    18541 ttagtattat ctgttgtgat tgtgattgca attttcatgc aaccaaccaa aaaccaatcc
    18601 agcaatgtat ttgatgccag ttcaggtgat ttgtttgaac gcagtaaagc tcgcggtttt
    18661 gaagctgtaa tgcagcgttt gacagggatt ttagtctttt tctggctagc cattgcctta
    18721 gcattgacgg tattatcaag tagataagaa aataatgggc aggactaggt ctttgcctct
    18781 ttttattttt aaaggatgtt tgagaaggtt ttacagtaaa agaaaattaa aaaatctaga
    18841 aagaaaatat gaaagataga ataaaagaat atttacaaga caagggaaag atgactgtta
    18901 atgatttggc tcaggctttg ggaaaagaca gttccaagga ttttcgtgag ttgattaaaa
    18961 ccttgtcctt aatggaaaga aagcaccaaa ttcgttttga agaagatggt agtctgacat
    19021 tagaaattaa gaaaaaacat gagattaccc tcaaggggat ttttcatgcc cataaaaatg
    19081 gctttggctt tgttagtctg gaaggcgagg aggacgacct ttttgtaggg aaaaatgatg
    19141 tcaactatgc tattgatggt gataccgtcg aggtagtgat taagaaagtc gctgaccgca
    19201 ataagggaac agcagcagaa gccaaaatta ttgatatcct agaacacagt ttgacaacag
    19261 ttgtcgggca aatcgttctg gatcaggaaa agcctaagta tgctggctat attcgttcaa
    19321 aaaatcagaa aatcagtcaa ccgatttatg ttaagaaacc agccctaaag ttagaaggaa
    19381 cagaagttct caaggtcttt atcgataaat acccaagcaa gaaacatgat ttctttgtcg
    19441 cgagtgttct cgatgtagtg ggacactcaa cggatgtcgg aattgatgtt cttgaggtct
    19501 tggaatcaat ggacattgta tccgagtttc cagaagctgt tgttaaggaa gcagaaagtg
    19561 tgcctgatgc tccgtctcaa aaggatatgg aaggtcgtct ggatctaaga gatgaaatta
    19621 cctttaccat tgacggtgcg gatgccaagg acttggacga tgcagtgcat atcaaggctc
    19681 tgaaaaatgg caatctggag cttggggttc acatcgcaga tgtttcttat tatgtgaccg
    19741 aggggtctgc ccttgacaag gaagccctta accgtgcgac ttctgtttac gtgacagacc
    19801 gagtggtgcc aatgcttcca gaacgactat caaatggcat ctgctctctc aatccccaag
    19861 ttgaccgcct gacccagtct gctattatgg agattgataa acatggtcgt gtggtcaact
    19921 ataccattac acaaacagtt atcaagacca gttttcgtat gacctatagc gatgtcaatg
    19981 atatcctagc tggcgatgaa gaaaagagaa aagaatatca taaaattgta ttaagtatcg
    20041 aactcatggc caagcttcat gaaactttag aaaacatgcg tgtgaaacgt ggagctctca
    20101 attttgatac caatgaagcg aagattttag tggataaaca aggtaagcct gttgatatcg
    20161 ttcttcggca gcgtggtatt gccgagcgga tgattgagtc ttttatgttg atggctaatg
    20221 aaacagttgc cgaacatttc agcaagttgg atttgccttt tatctatcga attcacgagg
    20281 agcctaaggc tgaaaaggtt cagaagttta ttgattatgc ttcgagtttt ggcttgcgca
    20341 tttatggaac tgccagtgag attagtcagg aggcacttca agacatcatg cgtgctgttg
    20401 agggagaacc ttatgcagat gtattgtcca tgatgcttct tcgctctatg cagcaggctc
    20461 gttattcgga gcacaatcac ggccactatg gactagctgc tgactattat actcacttta
    20521 ccagtccaat tcgtcgttat ccagaccttc ttgttcaccg tatgattcga gattacggct
    20581 gttctaagga aatagcagag cattttgaac aagtgattcc agagattgcg acccagtctt
    20641 ccaaccgtga acgtcgtgcc atagaagctg agcgtgaagt cgaagccatg aaaaaggctg
    20701 agtatatgga agaatacgtg ggtgaagagt atgatgcagt tgtatcaagt attgtcaaat
    20761 tcggtctctt tgtcgaattg ccaaacacag ttgaaggctt gattcacatc actaatctac
    20821 ctgaatttta tcatttcaat gagcgtgatt tgactcttcg tggagaaaaa tcaggtatca
    20881 ctttccgagt gggtcagcag atccgtatcc gtgttgaaag agcggataaa atgactggag
    20941 agattgattt ttcattcgta cctagtgagt ttgatgtgat tgaaaaaggc ttgaaacagt
    21001 ctagtcgtag tggcagaggt cgtggttcaa atcgtcgttc ggataagaag gaagacaaga
    21061 gaaaattagg acgctcaaat gataagcgta agcattcaca aaaagacaag aagaaaaaag
    21121 gaaagaaacc tttttacaag gaagtagcta agaaaggagc caagcatggc aaagggcgag
    21181 ggaaaggtcg tcgcacaaaa taaaaaggca cgccacgact atacaatcgt agatacgcta
    21241 gaggcaggga tggtcctgac tggaactgaa atcaagagtg tacgagctgc tcgaattaat
    21301 ctcaaggatg gctttgctca agtgaaaaat ggagaagttt ggctgagcaa tgttcatatc
    21361 gcgccttacg aagagggcaa tatctggaac caggaaccag aacgtcgtcg taaactcctg
    21421 cttcataaaa agcaaattca aaaattggaa caagagacca aagggacagg aatgacctta
    21481 gttcccctta aggtctatat aaaagatggc tacgctaagc ttcttttagg acttgccaaa
    21541 gggaagcatg actatgacaa acgggagtct atcaaacgtc gtgagcaaaa tcgagatatc
    21601 gcgcgtgtga tgaaagctgt taatcagcga taaaaagagg aattgaaaat ggaaaaatta
    21661 gttgcctata aacgcatgcc tttgtggaat aaacaaacaa tgcctgaagc tgttcagcaa
    21721 aagcacaata caaaagttgg gacttggggg aaaattactg tcttgaaggg agctctcaag
    21781 tttattgaat tgacagaaga aggggaagtt ctagctgaac acctctttga agcaggggca
    21841 gacaatccaa tggcccaacc tcaagcctgg caccgagtgg aagctgccac agatgatgtg
    21901 gaatggtact tggaatttta ttgtaaacct gaggattatt ttgctaaaaa atacaatacc
    21961 aatcctgttc attcagaggt cctagaggcc atgcagacag tgaaacaagg gaaagctttg
    22021 gatttgggtt gtggtcaggg gcgtaattct ctttttctag cccagcaaga ttttgatgtg
    22081 acggctgtag atcaaaatgg actagctctt gaaatcttgc aaagcattgt ggagcaggaa
    22141 gatttggaca tgcctgttgg cctttacgat atcaattcag ctagcattga acaagaatat
    22201 gattttatcg tttcaacagt tgttctcatg tttctacaag cggaccgcat tccagctatt
    22261 attcaaaata tgcaggagaa aaccagtgtt ggtggttaca accttatcgt ttgtgccatg
    22321 gacacggagg attatccttg ctcggttaac ttcccattca cctttaaaga aggagaactg
    22381 gcagactatt acaaggattg ggaattggtt aagtacaatg aaaatccagg ccatttgcac
    22441 cgtcgcgatg agaatggcaa tcgtattcaa ctacgctttg cgaccttact agctaagaaa
    22501 atcaagtaaa cacacatgaa gattaggaat tttcctgatc ttttttcttt tttacgaatg
    22561 atatagaaaa ggagggaatt catgtttgtt gcgagagatg ctaggggaga attggtaaat
    22621 gtgttagagg ataaacttga gaagcaagca tacacctgcc cagcttgtgg aggccagatc
    22681 catttgcgtc aaggaccaag tgtacggacg cattttgccc ataaatcctt aaaagactgt
    22741 gattttttct ttgaaaatga aagtccagaa cacatagcca ataaggaatc cctctatcac
    22801 tggttgaaaa aagagacaaa ggttcaatta gagtacccgc tttcagaact taaacagatt
    22861 gcggatgtat ttgtaaatgg caatctagct ctagaagttc agtgtagtcc cttgcctcag
    22921 aaagtcctta aagagcgaag tgagggctat cgtagtcagg gttaccaagt actgtggttg
    22981 ctgggtcaaa aactgtggct caaggagcgt ttgactcgtc tacagcaagg ttttctttat
    23041 ttcagtcaaa acatgggctt ttatgtttgg gaattagaca aggaaaaaca agttttaaga
    23101 ctcaaatacc tgatttacca ggatctccgc ggtaaactcc attatcaaat caaggaattt
    23161 tcctatggtc aaggtagttt attggaaata ttgcgtcttc cctataagag acaaaaaata
    23221 tctcatttta cagtttctga ggacaaggac atctgtcgct atatccggca acaactttat
    23281 tatcaaaatc tcttttggat gaaagaacaa gcagaagcct atcaaaaggg agaaaatatc
    23341 ctgacttatg gactgaaaga atggtatcca caaattcgac caatagtggg caaatttttc
    23401 cagattgaac aagacttgac tagctattat cagcactttt atacctatta ccaaaaaaat
    23461 cctcaaaatg attggcaaaa gctttatcca ccagcctttt atcagcaata tttcttgaaa
    23521 aatatggtag aatagaaagg atggaggaat ctaatggtat tacaaagaaa tgaaataaat
    23581 gaaaaagata catgggatct atcaacgatc tacccaactg accaggcttg ggaagaagcc
    23641 ttaaaagatt taacagaaca attggagaca gtagcccagt atgaaggcca tctcttggat
    23701 agtgcggata acctactaga aatcactgaa ttttctcttg aaatggaacg ccagatagag
    23761 aagctttacg tttatgctca tatgaagaat gaccaggata cacgtgaagc taagtatcaa
    23821 gagtactatg ccaaggccat gacactctac agccagttag accaagcctt ttcattctat
    23881 gagcctgaat ttatggagat tagcgaaaag cagtatgctg actttttaga agctcaacca
    23941 aagctgcagg tttatcaaca ctattttgac aagctcttgc aaggcaagga tcacgttctt
    24001 tcacaacgtg aagaagaatt attggctgga gctggagaaa tctttggttc agcaagtgaa
    24061 accttcgcta tcttggacaa tgcggatatt gtgttccctt atgtcctaga cgatgatggt
    24121 aaagaagttc agctatctca tgggacttac acacgtttga tggagtctaa aaaacgtgag
    24181 gttcgccgtg gtgcctatca agctctttat gcgacttacg aacaattcca acacacctat
    24241 gccaaaacct tgcaaaccaa tgttaaggtg caaaattacc gtgctaaagt tcgtaactac
    24301 aagagtgctc gtcatgcagc cctcgcagcg aattttgttc cagaaagtgt ttatgacaat
    24361 ttggtagcag cagttcgcaa gcatttgcca ctcttacatc gctatcttga gcttcgttca
    24421 aaaatcttgg ggatttcaga tctcaagatg tacgatgtct acacaccgct ttcatctgtt
    24481 gaatacagtt ttacctacca agaagccttg aaaaaagcag aagatgcttt ggcagtcttg
    24541 ggtgaggatt acttgagccg tgttaaacgt gccttcagcg agcgttggat tgatgtttac
    24601 gaaaatcgag gcaagcgttc aggtgcctac tctggtggtt cttacgatac caatgccttt
    24661 atgcttctca actggcagga caatctggac aatctcttta ctcttgttca tgaaacaggt
    24721 cacagtatgc attcaagcta tactcgtgaa actcagcctt atgtttacgg agattactct
    24781 atctttttgg ctgagattgc ctcaactacc aatgaaaata tcttgacgga gaaattattg
    24841 gaagaagtgg aagacgacgc aacacgcttt gctattctca ataacttcct agatggtttc
    24901 cgtggaacaa ttttccgcca aactcaattt gctgagtttg aacacgccat tcaccaagca
    24961 gatcaaaatg gggaggtctt gacaagcgat ttcctaaata aactctacgc agacttgaac
    25021 caagagtatt atggtttgag taaggaagac aatcctgaaa tccaatacga gtgggctcgc
    25081 attccacact tctactataa ctactatgta tatcaatatt caactggctt tgcggccgcc
    25141 tcagccttgg ctgaaaaaat tgtccatggt agtcaagaag accgtgaccg ctatatcgac
    25201 tacctcaagg caggtaagtc ggactatcca cttaatgtca tgagaaaagc tggtgttgat
    25261 atggagaagg aagactacct caacgatgcc tttgcagtct ttgaacgccg tttaaatgag
    25321 tttgaagccc ttgttgaaaa attaggattg gcataaaatg gttgaatcgt atagtaagaa
    25381 tgctaaccat aacatgcgtc gtcctgtcgt caaagaagaa attgtagact tgatgcgtca
    25441 gcgtcaaaag caggtcacag gtttcttgaa agaattggaa gactttgccc gcaaggaaaa
    25501 tattcctatt attccccatg aaacggttgc ttatttccgt tttattatgg aaaccatgca
    25561 gcctaaaaat attctggaaa ttgggacggc tatcggtttt tcagctctct tgatggctga
    25621 acatgcgcca aatgctaaga ttacaactat tgatcgtaat ccagaaatga ttggttttgc
    25681 caaggaaaat tttgcccagt ttgacagtcg caagcaaatc actctcctag agggagatgc
    25741 ggtggatgtc ttatctacac tgacagagtc ttatgatttc gtatttatgg attctgccaa
    25801 gtctaaatac atcgtctttc tgccagaaat cctcaaacat ttggaagttg gtggtgtggt
    25861 tgtcttggat gatatttttc aaggtggtga tgttgccaag gatattatgg aagtccgtcg
    25921 tggtcagcga accatttatc gaggccttca aaaattattt gatgcaacct tagacaatcc
    25981 agaactcacc gcaacattag tgcctttagg agatggtatt ctcatgcttc gtaaaaatgt
    26041 agcagatgtt caactgtctg aaagcgaatg attttcagaa aaatttaaga aaaaatagta
    26101 aaatagatag agtaacactt atctcaaagg agtagacatg aagaaaaaat tattggcagg
    26161 tgccatcaca ctattatcag tagcaacttt agcagcttgt tcgaaagggt cagaaggagc
    26221 agaccttatc agcatgaaag gggatgtcat cacagaacat caattttatg agcaagtgaa
    26281 aaacaaccct tcagcccaac aagtcttgtt aaatatgacc atccaaaaag tttttgaaaa
    26341 acaatatggc tcagagcttg atgataaaga ggttgatgat actattgccg aagaaaaaaa
    26401 acaatatggc gaaaactacc aacgtgtctt gtcacaagca ggtatgactc ttgaaacacg
    26461 taaagctcaa attcgtacaa gtaaattagt tgagttggca gttaagaagg tagcagaagc
    26521 tgaattgaca gatgaagcct ataagaaagc ctttgatgag tacactccag atgtaacggc
    26581 tcaaatcatc cgtcttaata atgaagataa ggccaaagaa gttctcgaaa aagccaaggc
    26641 agaaggtgct gattttgctc aattagccaa agataattca actgatgaaa aaacaaaaga
    26701 aaatggtgga gaaattacct ttgattctgc ttcaacagaa gtacctgagc aagtcaaaaa
    26761 agccgctttc gctttagatg tggatggtgt ttctgatgtg attacagcaa ctggcacaca
    26821 agcctacagt agccaatatt acattgtaaa actcactaag aaaacagaaa aatcatctaa
    26881 tattgatgac tacaaagaaa aattaaaaac tgttatcttg actcaaaaac aaaatgattc
    26941 aacatttgtt caaagcatta tcggaaaaga attgcaagca gccaatatca aggttaagga
    27001 ccaagccttc caaaatatct ttacccaata tatcggtggt ggagattcaa gctcaagcag
    27061 tagtacatca aacgaatagt ccaaatcaat gagtcaggga aaaaactcga cttcaggaaa
    27121 aaatgaagca aacattccca caataaaacg catagtacaa ggtttgtact gccccccaaa
    27181 aaagttagac aattaattta tccgaaggat ttagttctgt attgcacaga gctaagtcct
    27241 tttagtttta tcttaattct cttattgttg taataatcaa tatagtctat aatggcttgt
    27301 tccaattgat taagtgattt aaatgtttgc tcatagccat aaaacatttc ggattttaaa
    27361 atgccaaaga aagattccat cctaccgttg tcttggctgt ttcctttgcg tgacatagat
    27421 gcttgaattc ccttactctc taggaagcga tgataagaat cgtgttggta ttgccagcct
    27481 tggtcactat ggagaatcgt attctcgtag tgcttctctt tgaatgcctg ttccaacatt
    27541 aacgatcaat caatttaatc atatacctaa gattagaatt gtttatccca aatttatttg
    27601 aaagcttctc taagctatat ccttgttttc taagttcata gatctgaact ttatcatcat
    27661 aattcaattt cataataaaa acaccccaaa agttagattt tttctgtcta acttttgggg
    27721 tgtagttcat gtacacctga tatgatgcgt tttataattt taaagacttt ttgaccagcc
    27781 tcatttttta acttgatact cagtgaaaag caaagattaa actaggaagc tagctgtagg
    27841 ctgctcaaag aacagctttg aggttgcaga taaaacttgt gaggtaacca acatatataa
    27901 tgtgaagctg acgtggtttg aatagatttt aggagggtat gagtctggaa gttttaatgg
    27961 ataatgcaag attccataga atgggtaagc tagagttctt atgtgaagag tttgggcata
    28021 aacttttacc ttttcctccc tactcatctt agtatagaaa agtgaatctg aaatagtaca
    28081 taactgcttc taaaacattc ttataaattg atttaaattc tcaaatcata ttattcagtt
    28141 cttatttcat tttgctctac aatcctgttg agaagacacg tgttcatatc aaaaaggtat
    28201 tggcaagttg caataccttt ttacaaggtt cttttgtctt atttttgttt caactgacta
    28261 tatctcctat ggttctagtt cagaaggcta ggctataatt atgattgata agaagtatca
    28321 ttccaagtat tgagagtgaa tgtttcaaaa tcatgggttt ctataatggt caggctggca
    28381 tttgctagac cgccatcttt acgaagaagt ggttctttat agcctaggag agtacgaaga
    28441 ctggcagtaa gattggcgcc gtgtccgaca attagaatac gctcagctgg actatctttt
    28501 aatgatttga taaattggat ggtccgctga gttgtactat agagggattc ggctccgaac
    28561 attcgagtgt caaattgagc aagatttgaa cgaaaagcct ggatttgttg cgggtaaata
    28621 gcttccaggg ttgcaatttt caaaccttct aacttcccaa gttgccattc acggagatta
    28681 ggaacgattt ctaaagaaca gggggtatgg agttgacttt ggataatctc agcagatttg
    28741 accgctcgag gtaaatcact tgaataaatc tgatcaaaag gaatttcctt gagatactgg
    28801 ccaagtcgtt ttagggtttc aatggattca ggaagaagag gagaatcacc actagcacct
    28861 tgaaaacgac cttcttggtt ccagagggta cgaccgtggc ggacaaagta gagtttcatt
    28921 acttgatgtc ctccaaaata tctacaaagt ctgcctttac aaagctagcc aagtcttgtg
    28981 gcgcgacgat aatgctgtgt ccgacttcgc ctgcagagac aatcatttga tccaaatcta
    29041 gagcaatttt atcgataaaa atgggataat tgtgtttctg acgaattccg acaggattat
    29101 tggctccatg aatgtaacca gttgtttttt ctaagtcctt ttgtggaatc atgctcactt
    29161 ttttattgcc agaaatttta gctagtttct tttcagacaa gtgctgagtg atagggacaa
    29221 ttccgataat cggtccggtc ttgtctccca aaagcgccaa ggttttgaaa atctgatctc
    29281 gttcataacc ttgaggaagc tctccttcta gggcattgat ttgaatcccc tgatgaggga
    29341 tagctgcttt agataggatt tgttccacca atgttttttt gattttaact ttttttgcca
    29401 ttatttatat ttatcctcca attgactcat ccaaatacca agccagattc ccagcgcaaa
    29461 gaagaaggcg atgatgacat aaccgacaag tgaaagtcct gtgtattgga tactttcagc
    29521 gtttcctgca tttggaatta agatcaaaag ggtacttgat aggacgatac cgatgatgaa
    29581 atgatagacg cgtgagtggt agttgtttaa ggcataatcc atcaattttg aaaaaacgat
    29641 gagagttgca cctgcaccaa ttccaatcgg aaagaaggtt cccaagagat caaaagtttt
    29701 aaaaccagtc aacataggag catagagtcc caaaatcaaa agtaaatttg atggactgag
    29761 gccaggaacc aagacgccaa gggccaatag tgcgcctgct aggacgaagt taagaaagct
    29821 ggcgcttaag gttccaacga caaaatttaa ggcatagagt cctaatccag aaatgataaa
    29881 ggttgtccat aaccaagcta aatcaatctt gtctcggtca gattctcgag ttgattcttt
    29941 gaggaggcta ggaactgtac cgataatagc tcccgcaaag ctccataata caaaaacctg
    30001 ataattttca agcaggtatt caatcgggta ggaaaataag ccgattccca gaagcatacc
    30061 gatggcaact ggaataaagt acaaaacatt ttctttaaag tctttaaagg gatgggccag
    30121 aaagccaatc attcgttcat agattcctaa gattgctgct agaacccctc cggaaattcc
    30181 cggtaggata aatccaagag caattacaat ccctttaata acgcgtgcta accatgagag
    30241 catatatttc ctccaactat ttctatttca aaaaatcctt actatattgt atcacaatca
    30301 gacacaaaaa gaaaaagcaa atgataaaca aatgctttta aggttttaaa aaaagttttc
    30361 gaaaggtttc ttctttattt tttaagggag agataacgtt gatatctaaa tcgtggtcaa
    30421 agccggcaat ttttccttta gatgtgtatt ggtgaatatc ataatctaaa tcagttttag
    30481 gactgctctc caaaaatcct gagtctgagc cgtaggacgg aatccaaaca gaggtaaact
    30541 tgcctgtatc aatactgtgt tcttccatga agtagacacc aacgtagatg ccgatgtttt
    30601 tagcacctag tgatgctagt tttgctcgaa agttttcgac accttcgttc atattagaca
    30661 tggttttgtc ttccacgtca agccaatagt aactagggct gtaaggagag gcagcattgt
    30721 agaaaacttc ggcagccttt tccatttctt ggacactttt tccagctaca taagcgtaga
    30781 cagcaactgg gacattccgc ttttgaagtt cagtgatatg actcttatag gccttgtcta
    30841 ttccattgat aaatgaagca tcattttctt ttgttgtttg agcaccactg tgaacacgaa
    30901 caatagcacc tgaaatattt tgtgagaggg catcgtagtt gatttcctca ggatgctgcc
    30961 agccagagag gtcaataatc ggtttgtcta agtgtttcaa agcctgtgct atattggatt
    31021 ttgttttaaa cgattggctg tcattaagtg ggcgattgat gattaaaatg aacatcataa
    31081 tcccaaaaaa actaaataaa ataagtggat gaatttgttt tctcatatct tataagtcta
    31141 ccctaaaaat caaaaaaaat caaaaaaaat caaaaaaatg ggttaaggaa gagactttag
    31201 agcatttttt cattcaagag tgcggaatga tttgaaatat ggtataataa aagggaattt
    31261 ctacagaaaa gagaagatta tgtcaaattt tgccattatt ttagcagcgg gtaaagggac
    31321 tcgcatgaaa tctgatttgc caaaagtttt gcacaaggtt gcgggtattt ctatgttgga
    31381 acatgttttc cgtagtgtgg gagctatcca acctgaaaag acagtaacag ttgtaggaca
    31441 caaggcagaa ttggttgagg aggtcttggc tggacagaca gaatttgtga ctcaatctga
    31501 acagttggga actggtcatg cagttatgat gacagaacct atcttagaag gtttgtcagg
    31561 acacaccttg gtcattgcag gagatactcc tttaatcact ggtgaaagct tgaaaaactt
    31621 gattgatttc catatcaatc ataaaaatgt ggccactatc ttgactgctg aaacggataa
    31681 tccttttggc tatggacgaa ttgttcgtaa tgacaatgct gaggttcttc gtattgttga
    31741 gcagaaggat gctacagatt ttgaaaagca aatcaaggaa atcaacactg gaacatacgt
    31801 ctttgacaac gagcgtttgt ttgaggcttt gaaaaatatc aataccaata acgctcaagg
    31861 cgaatactat attacagacg tcattggtat tttccgtgaa actggtgaaa aagttggcgc
    31921 ttatactttg aaagattttg atgaaagtct tggggtaaat gaccgtgtgg cgcttgcgac
    31981 agctgagtca gttatgcgtc gtcgcatcaa tcataaacac atggtcaacg gtgttagctt
    32041 tgtcaatcca gaagcaactt atatcgatat tgatgttgag attgctccgg aagttcaaat
    32101 cgaagccaat gttaccttga aagggcaaac gaaaattggt gctgagactg ttttgacaaa
    32161 tggtacttat gtagtggaca gcactatcgg agcaggagcg gtcattacca attctatgat
    32221 tgaggaaagt agtgttgcag acggtgtgac agtcggtcct tatgctcaca ttcgtccaaa
    32281 ttcaagtctg ggtgcccaag ttcatattgg taactttgtt gaggtgaaag gatcttcaat
    32341 cggtgagaat accaaggctg gtcatttgac ttatatcgga aactgtgaag tgggaagcaa
    32401 cgttaatttc ggtgctggaa ctattacagt caactatgac ggcaaaaaca aatacaagac
    32461 agtcattgga gacaatgtct ttgttggttc aaattcaacc attattgcac cagtagaact
    32521 tggtgacaat tccctcgttg gtgctggttc aactattact aaagacgtgc cagcagatgc
    32581 tattgctatt ggtcgcggtc gtcagatcaa taaagacgaa tatgcaacac gtcttcctca
    32641 tcatcctaag aaccagtagg agcctatcat ggagtttgaa gaaaaaacgc ttagccgaaa
    32701 agaaatctat caaggaccaa tatttaaact ggtccaagat caggttgaat taccagaagg
    32761 caagggaact gcccaacggg atttgatttt ccacaatggg gctgtctgtg ttttagcagt
    32821 aacggatgaa caaaaactta tcttggtcaa gcagtaccgc aaagctatcg aggctgtctc
    32881 ttacgaaatt ccagccggaa aattggaagt aggagaaaac acagcccctg tggcagctgc
    32941 ccttcgtgaa ttagaggaag aaacagccta tacagggaaa ttagaactct tgtacgattt
    33001 ttattcagct attggctttt gtaatgagaa gttaaaacta tatttagcaa gcgatttaac
    33061 aaaagtggaa aatccgcgtc cgcaggatga gaatgaaacc ttggaagtcc ttgaagtgag
    33121 cttagaagaa gcgaaagaat taatccaatc aggtcgtatt tgtgatgcca agacaattat
    33181 ggctgttcag tattgggagt tgcagaaaaa atagaggagg tcagtatggg taaatcttta
    33241 ttaacggatg aaatgattga aagagctaat agaggcgaaa aaatttcagg tcctcctttg
    33301 ctagatgata atgaggaaac taagatttta ccaacctctt cttcccgttt tggttatgcc
    33361 aatcctaagg atcatggttt tagccaggaa accttgaaga ttcaggtcga accatctatt
    33421 cataaaagcc gtcgtattga aaataccaag agaaatgtct tcaattctaa gttgaataaa
    33481 atcttatttg cggtcatctt tctcttgatt ttgcttgttt tagcaatgaa acttttgtaa
    33541 tagaaaagga attgaaatga aaataggaat tattgctgct atgccggaag aactggctta
    33601 tctggtccag catttagata atgcccagga gcaagttgtt ttggggaata cctatcatac
    33661 aggaaccatt gcttctcatg aagtcgttct tgtagaaagt ggaattggta aggtcatgtc
    33721 tgctatgagt gtggcgattt tggctgatca tttccaggtg gatgccctta ttaatacggg
    33781 ttcagctggg gcagtagcag aaggtatcgc tgttggggat gtcgtgattg ctgacaaatt
    33841 agcctatcat gacgtggatg tcacagcttt tggctatgct tatggacaaa tggcgcaaca
    33901 accgctttat ttcgaatcag acaaaacctt tgttgctcaa atccaagaga gtttatctca
    33961 attggaccaa aactggcatc ttggtttgat tgctacagga gatagttttg ttgcaggaaa
    34021 tgacaagata gaagcgatta agtcccattt cccagaagtt ttagccgtgg agatggaggg
    34081 ggcagctatt gctcaagcag cgcatgccct caatctccca gtcttagtca tccgagctat
    34141 gagtgacaat gccaaccatg aagcaaacat cttttttgat gagtttatta tcgaagctgg
    34201 acgtcgctct gcccaagtct tgttggcctt tttgaaggct ttagattaag cggaaatttg
    34261 acagtttttc tagcttatga taagatttaa gtaaagaaaa gctagaaaac gtttcagagg
    34321 atattatgag tattgaaatg accgtcagtg agattgcaga ggtcttagga ttatctcgcc
    34381 aagcaatcaa taaccgtgtc aaagaattac cagaagaaga cacagataaa aatgacaaag
    34441 gggtaacagt tgttaccaga agtggcttga ttaagctaga agaaatctat aaaaaaacga
    34501 tttttgaaga tgagcctgtc agtgaagatg tcaaacaacg tgaactgatg gagattctag
    34561 tggatgagaa gaatgcagaa attcttcgtc tttatgaaca attaaaagcc aaggatcgtc
    34621 agttatcaga aaaagacgag cagatgcgta tcaaagaccg tcagattgct gagaaagaca
    34681 aacaattaga tcagcaacaa caattgaccc ttcaagccat gaaggatcaa gaaaatctta
    34741 agctagagct ggaccaagca aaagaagaag tccaatccac taaaaaaggc ttttttgctc
    34801 gtttatttgg aggataatca aggagctgtt taggttaaat gctaacctga tggcttcttt
    34861 tgtttgtgaa tagtatagtt gattagaaca gagacagtag aggagaggta cagaatggaa
    34921 cgattagaag attacattgc ttttgattta gaatttaatc agcacgaagg acaaacacac
    34981 ttgattcaag catcggcagt gcgttttaga gatggtcggg aaatcgatgc ctatgattcc
    35041 tatgttcata ccagcgtccc tctaaaaagc tttatcaatg gtttgaccgg gattacagct
    35101 gagaccttaa aagatgctcc tccagttgag aaagtcttag aagaattcca agagtttgtg
    35161 ggttctttgc tgatgatagg ctacaatgct gctaaaagtg atttacctat tttagcagag
    35221 catggtttag actatagcca acagtacaag gtggatttgt atgatgaagc ttttgaacgt
    35281 cggagttcag atttacatgg tattgccaat ctcaaattgc aaactgttgc ctcatttctt
    35341 ggatttcaag gtcagtctca taatagttta gaggatgctc ggatgacggc gcgtgtttac
    35401 gaatccttct tagagtcgga tcaagctagg gaattgttag agacagaaag tagcttatca
    35461 aacaatcctt ttggtggctt ggacttgtct caattatttg actaggagtg cctatgaaac
    35521 ctgaaaaacc taaaaaattg ggtcttaaac agatttacct caatcttgat aaaatcctct
    35581 ttctattttt cttgattttt atgatagtgg agtatttgtg ggtaccattc aattcatggt
    35641 tggctagctt cttattagat cagacaggtt atctttttat ttcctataac aatgtcttag
    35701 ccatccttac tcattctcca ttgattagtc tagctttttt agccttgatt gtaattaacc
    35761 ttctggttgc ttattttcaa ataggactcc tctttattgg tgctcgtcat ctcctctata
    35821 atgaaaagcg aaggctaatt gagtatatcc gcaaggtttt ccatgaaagt tttggattta
    35881 tgaaaaagct aacttgtgct aaagccttgt ttatcttttt ctatatagct atgctttttc
    35941 cttttatacg gaaaatgctg aagatttatt atcttaataa aatcgtcatt ccagagttta
    36001 tccaagctta tttagaagat aaatattgga tgtggtggct gagcatccta cttctatctc
    36061 ttatctttct ctatgtctct gtcagattga tgtttgccct tccgaagatt gtatatgatc
    36121 agctgactgt tcgagaagca gtgatgttta gcttggagaa aaccaagaag agagttactt
    36181 tttatgcttg gaatttattt tacatcttgc tcaaagcaca tttattattt tatcttcctt
    36241 tgattccttt attattggct caaactttgg tagatgatat cactcagaga gagtctctga
    36301 ttcttggtat tttgaatttt gtcgtgatta aaaatctcta ttatatggct ctgacttatt
    36361 ttttggttaa atttgtttca tttttgacag gaaaggagct ggatatgctt cctaggagag
    36421 aaaaagatca catcgtgcga tggggtgtca tgacttgtgc cagtcttttc tttgccttag
    36481 aaggttatat ttatctggag gctcccatgg ttcatctacc tcaacttatt tctcaccgag
    36541 gggtttccaa tgcaaatggg attcaaaata cagtagagtc cttggaaact acagcacaac
    36601 tcaaaccaga cttggtggag acggacgtgc aggaaacaaa agatgggcag tttgtcatga
    36661 tgcatgatgc taacttgaaa aatctagcag gtatcaataa aagtcctcaa gacttaaact
    36721 tggaggagct taaagggatt gatatttttg aaaatggcta ccagactaaa atttcaagct
    36781 ttgaagatta tctcagtcga gccaacgaac ttggtcaaaa attactaatt gaaattaaaa
    36841 ccagtaaaaa agatagtcca gacatgatga accgcttttt agcccgttat gctgcaaagc
    36901 tcaagattta tggacatcaa atccagtctt tagactacca tgttgtcgaa aaagtaagac
    36961 agtatgatgc cgaactgcca gtttatttca tcatgcccta caattctgta tttcctaaaa
    37021 caagagcgac aggatatact atggagtact caaccttgga tgaatatttt gtaagcaaac
    37081 tatggacaac ggatcagaaa ctttatgtct ggactgtcaa tgattcagaa gctatcagca
    37141 aatctcttca cttaggagta gatggagtga ttacagatga tttggaaaaa gttcagcggg
    37201 aaatagaagt agcccaagaa gatccagaat atacggattt gctcttgaaa aaagcattgg
    37261 aattctttga attttagtaa taaaaaagag gaactgttgt tcctcccatg ataggtaagt
    37321 attcttgtga atgcttgctt ttttgtggtt gcaatccgtt tacgagaatt gtttgtattg
    37381 ttcttgtttc aatttacttt atagtgcttt gaagttggaa tgctacacta taacttctaa
    37441 aatatagtaa aatgaaccaa aaatag
//
LOCUS       DAVKGU010000010        37013 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKGU010000010 DAVKGU010000000
VERSION     DAVKGU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.010"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(745..1593)
                     /locus_tag="VW077_000479"
     CDS             complement(745..1593)
                     /locus_tag="VW077_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9454556.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNHHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAS
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1745..2023
                     /locus_tag="VW077_000480"
     CDS             1745..2023
                     /locus_tag="VW077_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454557.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            2025..4088
                     /locus_tag="VW077_000481"
     CDS             2025..4088
                     /locus_tag="VW077_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9454558.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(4166..5422)
                     /locus_tag="VW077_000482"
     CDS             complement(4166..5422)
                     /locus_tag="VW077_000482"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9454559.1"
                     /translation="MHIFDELKDRGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADTVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5564..8026
                     /locus_tag="VW077_000483"
     CDS             5564..8026
                     /locus_tag="VW077_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9454560.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSRARR"
     gene            8091..9002
                     /locus_tag="VW077_000484"
     CDS             8091..9002
                     /locus_tag="VW077_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9454561.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            9232..9930
                     /gene="dapD"
                     /locus_tag="VW077_000485"
     CDS             9232..9930
                     /gene="dapD"
                     /locus_tag="VW077_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9454562.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9998..11128
                     /locus_tag="VW077_000486"
     CDS             9998..11128
                     /locus_tag="VW077_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9454563.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            11140..11679
                     /locus_tag="VW077_000487"
     CDS             11140..11679
                     /locus_tag="VW077_000487"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9454564.1"
                     /translation="MKSELRKQVLQGMKAISQEQKQFIDQTLTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDLQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11663..12340
                     /locus_tag="VW077_000488"
     CDS             11663..12340
                     /locus_tag="VW077_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9454565.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(12407..13306)
                     /gene="galU"
                     /locus_tag="VW077_000489"
     CDS             complement(12407..13306)
                     /gene="galU"
                     /locus_tag="VW077_000489"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9454566.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKTVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(13328..14344)
                     /locus_tag="VW077_000490"
     CDS             complement(13328..14344)
                     /locus_tag="VW077_000490"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9454567.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            14556..14931
                     /locus_tag="VW077_000491"
                     /pseudo
     CDS             14556..14931
                     /locus_tag="VW077_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(15111..15536)
                     /locus_tag="VW077_000492"
     CDS             complement(15111..15536)
                     /locus_tag="VW077_000492"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9454568.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(15604..16254)
                     /gene="phoU"
                     /locus_tag="VW077_000493"
     CDS             complement(15604..16254)
                     /gene="phoU"
                     /locus_tag="VW077_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9454569.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKTLLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLSDLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(16269..17021)
                     /gene="pstB"
                     /locus_tag="VW077_000494"
     CDS             complement(16269..17021)
                     /gene="pstB"
                     /locus_tag="VW077_000494"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9454570.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWDEVKDNLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(17023..17838)
                     /gene="pstA"
                     /locus_tag="VW077_000495"
     CDS             complement(17023..17838)
                     /gene="pstA"
                     /locus_tag="VW077_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9454571.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17831..18694)
                     /gene="pstC"
                     /locus_tag="VW077_000496"
     CDS             complement(17831..18694)
                     /gene="pstC"
                     /locus_tag="VW077_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9454572.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18812..19687)
                     /locus_tag="VW077_000497"
     CDS             complement(18812..19687)
                     /locus_tag="VW077_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU9454573.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTTKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19854..21185)
                     /locus_tag="VW077_000498"
     CDS             complement(19854..21185)
                     /locus_tag="VW077_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9454574.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(21186..21893)
                     /locus_tag="VW077_000499"
     CDS             complement(21186..21893)
                     /locus_tag="VW077_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9454575.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21977..22279)
                     /locus_tag="VW077_000500"
     CDS             complement(21977..22279)
                     /locus_tag="VW077_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9454576.1"
                     /translation="MTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(22266..22487)
                     /locus_tag="VW077_000501"
     CDS             complement(22266..22487)
                     /locus_tag="VW077_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9454577.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            22449..23006
                     /locus_tag="VW077_000502"
                     /pseudo
     CDS             22449..23006
                     /locus_tag="VW077_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23321..25012)
                     /locus_tag="VW077_000503"
     CDS             complement(23321..25012)
                     /locus_tag="VW077_000503"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9454578.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            25148..25594
                     /gene="argR"
                     /locus_tag="VW077_000504"
     CDS             25148..25594
                     /gene="argR"
                     /locus_tag="VW077_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9454579.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            25645..28179
                     /gene="mutS"
                     /locus_tag="VW077_000505"
     CDS             25645..28179
                     /gene="mutS"
                     /locus_tag="VW077_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9454580.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMENPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            28326..30020
                     /gene="patA"
                     /locus_tag="VW077_000506"
     CDS             28326..30020
                     /gene="patA"
                     /locus_tag="VW077_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9454581.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(30350..30613)
                     /locus_tag="VW077_000507"
                     /pseudo
     CDS             complement(30350..30613)
                     /locus_tag="VW077_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            30811..32577
                     /gene="patB"
                     /locus_tag="VW077_000508"
     CDS             30811..32577
                     /gene="patB"
                     /locus_tag="VW077_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9454582.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            32716..33405
                     /locus_tag="VW077_000509"
     CDS             32716..33405
                     /locus_tag="VW077_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9454583.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            33409..33588
                     /locus_tag="VW077_000510"
     CDS             33409..33588
                     /locus_tag="VW077_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454584.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            33575..34924
                     /locus_tag="VW077_000511"
     CDS             33575..34924
                     /locus_tag="VW077_000511"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9454585.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            35048..36508
                     /locus_tag="VW077_000512"
     CDS             35048..36508
                     /locus_tag="VW077_000512"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9454586.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            36522..36953
                     /locus_tag="VW077_000513"
     CDS             36522..36953
                     /locus_tag="VW077_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9454587.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
BASE COUNT        10802 a         7059 c         8133 g        11019 t
ORIGIN      
        1 atataaatgg acggatttat tttggatttt atttgttatt ttgacctgcc tctatattgg
       61 taaccatgat ttgtttactc tcaatcatca agaattctct tttcgtggta gcgtttgggg
      121 tctggtactg gccttatatc acttactatt cattgataag tttgttatat cgaatcgaaa
      181 ataaagatta gagctatgct tgactggcta cttttaggat tgattttgga ggaagatttt
      241 gtctctatta tttattattt taaatttatt tattttgtat aagatctatt ctttgaggcg
      301 ggaaaaatcg aaatacttga tttatacggc ctatatcata tttggggtaa atgtagtata
      361 tggtattcaa tggttattaa aagaactgat ttcaactatt tccccttaat gtgtatggaa
      421 actataacaa aacgcataag attaaggttt gtgtacctgt tcttatacgt ttttgtcata
      481 ttgaagattt tcggtaagtt ttctactttt gcgaagtcta gtttatactc aatgaaaatc
      541 aaagagtaaa ctagccgtag gttgctcaaa gtacagcttt gaggttgcag ataaaactga
      601 cgaagtcagc tcaaaacatg gttttgaggt tgtagatgaa actgacgaag tcagctcaaa
      661 acatggtttt gaggttgtag atgaaactga cgaagtcagt aaccatacgt acggcaaggc
      721 gacgttgacg tggtttgaag agtattagaa tgctttccca accaaaatct ctgcttcgat
      781 ggtaatctct gtcagttggc tccagtcaat cttgctctgg tcaacgtgaa agaggagagg
      841 agtcatactg agtagggctt ggagttgttc tgctgtgata gtcttagtca gagaggcagt
      901 ttgactagat aggatggtaa agtgttcctg gaaatgctcc ttgatatctt ggttagaata
      961 ctccttgttt gtcagctggt cctgtactct ttgacggatt tctttgaggt gattttcagt
     1021 tgggataacc tttatcaaga taccgtcttt ggataaaacg cgacgaaatt ctccatagtt
     1081 ggcaggtgag aagatatcaa gcaggatatc catgctagcg tcttttatag gaaggcgtgc
     1141 caagtcgcca acgaaccaat tgactgccca gttgggttca cttttagcag cgatttggac
     1201 tgaatctttg gagatgtcaa aggcatagaa agttttttca gagtgacttt cttgtagttt
     1261 gcgagaatag aatccttcac cacaaccgat atccaaaatt gttgtggtag tttttgagct
     1321 tgcaagcaag tcagatacag catctaagat agcttggtaa aagccggctt ctaggatttg
     1381 ttgacggttt tgaaaatttt ccttgtcata gttagcagat tgcttgattt gaggtgctag
     1441 attgacatag ccaaatttcg ccaagtcaaa agaatgatgg ttgcagcact tgaaattagt
     1501 ctctaacaga gtcagatttt cttgacagat aggacaggca aaggcagtcg cagaagcaaa
     1561 acgctgaagt ttgggcttga gatttgtatt catagtttaa gtgtatcaaa agaggcaaat
     1621 gaaagcaact ttaggaataa gtagatggga gattcagtag aaataaaact aattctccaa
     1681 tttatagaat gaaattgctt ttatatctaa attgctatat aataatgata aggaggaaat
     1741 aagtatgtta aaagaagtat taaccgttgc aaaagttgcg aaaaaatctt cattattttt
     1801 gggtggtgtc gcatttggta cccttggttt gaaaatctta gcaagtaagg aagctaaaaa
     1861 aggttattct aaagctttgg ctaaggctta caagttgaaa gacgagctag atgcatctgt
     1921 ttctgtcgtg aagcaacatg gagacgatgt cttgcaagat gccaaatatt tgtacgagca
     1981 agagaaaaaa gaagagcaat tagatagcct tataggtgaa taatatgtct tttaaagtgc
     2041 tacatagagg ataccaacat atccgactat catcttcttt ttcacttacc ttggatattc
     2101 aagactatct tcgttccttg gcgagagatg aaaaggggat tgagtctatc cagttttaca
     2161 tggatcaaca gcactttact ctacgcataa aagaaggctt ttctgtatta gataatgcag
     2221 aagccttttt aaaaagaatt gataaaggga aagtttctga gttgatgact cttcccattc
     2281 gtagagaaga gagtgcttat tctattgttt caggtgcagc ggttaagcgt gtactttttc
     2341 gtagttttgt gccgtatcct attcgctata tatggacttg ttatcaggct ttgggttata
     2401 ttagagaagc ctatcaaaca ctagcgcgta aggaactaac gatggaagtc ttggactgtt
     2461 cggctatttt attgtctttg tttatgaacc aatccaagac agctagcaat atcatgttta
     2521 tgcttgattt ggggaatcat ttagatcagt ggtccttgaa aaaaactgca acagatttag
     2581 aacagagtct tcttgcaaaa gagagcgatg tattcctagt acagggcgat acggttgtta
     2641 gtatcaagag ttccgatgtt caaataggag atgtcttgat cttatctcaa ggaaatgaaa
     2701 ttctgtttga tggacaagta gtttcaggtt taggtatggt caacgaaagt tccttgacag
     2761 gagagagttt tccagttgaa aaaagagagt ctgatttggt ttgtgcaaat acagtattag
     2821 aaactggaga gttacgcatt cgtgtaacag ataatcagat gaacagccgt attttacagc
     2881 tgattgagtt gatgaagaaa tctgaagaaa acaagaaaac gaaacaacgc tatttcatca
     2941 agatggcgga taaagtcgtc aaatataatt tcttgggggc tgggctgact tacctattga
     3001 caggttcttt ttctaaggct atttctttcc tattggtcga tttctcctgc gctttgaaaa
     3061 tctctactcc tgtagcttat ttgacagtta tcaaggaagg gttgaaccgt gaaatggtga
     3121 ttaaggatgg agatgttctg gagaaatatc tggaagttga tactttcttg tttgataaga
     3181 caggaacaat cacaactagt tatcctatag ttgaaaaggt gttacctttt ggagactata
     3241 gtgaggaaga tattctcaga atcagtgcct gtcttgagga acacatttat catcctattg
     3301 ctaatgccat cgtcaagcaa gctgagatag agggaattga acatgaggaa atgcatggga
     3361 aactccaata tatcgcaagc aaggggatca aatctcatat agatggccaa ccagttctta
     3421 ttgggaatta tgtcttgatg caggatgagc agattcatat cagttcagaa caaaatgctt
     3481 taattgaaga gtacaagagt cactacaatc tcttattctt ggcttatcaa aatgaattga
     3541 ttggaatgtt ctgcattcat actcctttga gaaaagaagc aaaaacagcc ttggataaac
     3601 ttaaggcaca ggggaaaaaa ttgattctgg caacaggaga caccttgatt aggacagagg
     3661 aattagtcaa agatttgccc tttgatcagg tctatacaga cttgaaacct gatgggaaat
     3721 ttgagttagt agagaaactg cagaaagcag gtcacactat tttgatggtt ggagatggct
     3781 tgaatgactc agcggcttta actctatcag atatcggtgt ggtgatgaat gagagtgcag
     3841 atatttctaa gcagatgagt gatatcttat tgttagataa tcgtttggat ttcttccaag
     3901 agttggattc gttatcatca tctttgcaaa cactcatcaa gaaaaatatt caggataccg
     3961 ttgtcgtaaa tagtagtttg attggctttg gcttatttaa ttggttcagt ccttcaaatc
     4021 tctctatctt acataatcta acaaccttgc gcattgtcct gcgtagcctg tctattaaaa
     4081 atagatagat gagagttatt aacagcaaaa aggagttacc tttctcgagg ttaactcctt
     4141 tgtttataga tagatgttga atagtttagt aagtcaatac aaagtatttt ttcttcccac
     4201 gacggataac agtcagttca ttttctaact tatcagcgtc actcaagaca tagtcaaggt
     4261 cttggatgcg gtcgccgttt acgtagatgg ctccgttttg gacgtcttca cgggcttggc
     4321 gttttgagtt aactatacca gatgagacga gcagttccac gatattgttg ttttcgtctg
     4381 cctgtacttg gtagtttggc acaccacgaa gtccttgttt gagctctttg acagaaaggt
     4441 ttttgatgtt tcctgcaaag agttgctcag tgatgttaag tgcttctttg taggcttctt
     4501 ctccgtgaac aagtgtaaca acttcacgag ccaagacttt ttgagccaag cgttcgtgtg
     4561 gcgctgcttc aaattgttta cgaatatctt caatctcatc aagtgacaag aaagtaaaga
     4621 ttttcaagaa gcgaacagtg tcagcgtcca tcacgttcat ccagaattgg tacatttcgt
     4681 atggagaagt cttttcggga ttaagccaga cggcatttcc ttctgattta ccaaatttct
     4741 taccagttgc atctgtgatt agtggaacag tgataacgtg accagtcttg tccgccttac
     4801 gacgaagcaa ttcggtacca gctgtcatat ttccccactg gtcagaacca ccgatttgaa
     4861 gagtgacatt atggtcttgg ttaaggacga agaagtcgta cccttgcatg atttggtaag
     4921 cgaactcagt gtaagaaatt cctgtttcga tccgtttttt aacagattcc ttactcatca
     4981 tgtagttgac cgtgaagtat tttccaatat cacggaggaa gtcaatgaag ctgatgctgc
     5041 caaaccagtc gtagttgttg accatgacag ccttgttttc gccattttca aagtcaagaa
     5101 aacgagaaag ttgtccttgg atagacttga cccagccatc tactgtgtct tttgtttgga
     5161 gactacgttc agcatctttg aaggacggat ctccgatgag acctgtagca ccgccaacga
     5221 gcgcataagg tttgtgacct gctagttgca agcgacgact tgtcaagatt gcgacaaggt
     5281 ggcctaggtg aaggctgtca gcagttggat cgtagccagt ataataagaa acttgacctt
     5341 cttctagggc tttacgcaaa gcttcttcat cagtcgtttg aaatatcaaa ccacgatctt
     5401 ttagctcatc aaaaatgtgc atgtgtcttt tctccttttt aaaattattg tttctaccta
     5461 ttgtatcaca aacttgggca atagcctagc agaataggga agaaagtggc tattttattg
     5521 aaaatttccc ttttgtggta taatagatag agtgaggaaa tccatgcaaa atcaattaaa
     5581 tgaattaaaa cgaaaaatgc tggaattttt ccagcaaaaa caaaaaaata aaaaatcagc
     5641 tagacctggc aagaaaggtt caagtaccaa aaaatctaaa acattagata agtcagccat
     5701 tttcccagct attttactga gtataaaagc cttatttaac ttactctttg tactcggttt
     5761 tctaggagga atgttgggag ctgggattgc tttgggatac ggagtggcct tatttgacaa
     5821 ggttcgggtg cctcagacag aagaattggt gaatcaggtc aaggacatct cttctatttc
     5881 agagattacc tattcggacg ggacggtgat tgcttccata gagagtgatt tgttgcgcac
     5941 ttctatctca tctgagcaaa tttcggaaaa tctgaagaag gctatcattg cgacagaaga
     6001 tgaacacttt aaagaacata agggtgtagt acccaaggcg gtgattcgtg cgaccttggg
     6061 gaaatttgta ggtttgggtt cctctagtgg gggttcaacc ttgacccagc aactaattaa
     6121 acagcaggtg gttggggatg cgccgacctt ggctcgtaag gcggcagaga ttgtggatgc
     6181 tcttgccttg gaacgcgcca tgaataaaga tgagatttta acgacctatc tcaatgtggc
     6241 tccctttggc cgaaataata agggacagaa tattgcaggg gctcggcaag cagctgaggg
     6301 aattttcggt gtagatgcca gtcagttgac tgttcctcaa gcagcatttt tagcaggact
     6361 tccacagagt cccattactt actctcctta tgaaaatact ggggagttga agagtgatga
     6421 agacctagaa attggcttaa gacgggctaa ggcagttctt tacagtatgt atcgtacagg
     6481 tgcattaagc aaagacgagt attctcagta caaggattat gaccttaaac aggacttttt
     6541 accatcgggc acggttacag gaatttcacg agactattta tactttacaa ctttggcaga
     6601 agctcaagaa cgtatgtatg actatctagc tcagagagac aatgtctccg ctaaggagtt
     6661 gaaaaatgag gcaactcaga agttttatcg agatttggca gccaaggaaa ttgaaaatgg
     6721 tggttataag attactacta ccatagatca gaaaattcat tctgccatgc aaagtgcggt
     6781 tgctgattat ggctatcttt tagacgatgg aacaggtcgt gtagaagtag ggaatgtctt
     6841 gatggacaac caaacaggtg ctattctagg ctttgtaggt ggtcgtaatt atcaagaaaa
     6901 tcaaaataat catgcctttg ataccaaacg ttcgccagct tctactacca agcccttgct
     6961 ggcctacggt attgctattg accagggctt gatgggaagt gaaacgattc tatctaacta
     7021 tccaacaaac tttgctaatg gcaatccgat tatgtatgct aatagcaagg gaacaggaat
     7081 gatgaccttg ggagaagctc tgaactattc atggaatatc cctgcttact ggacctatcg
     7141 tatgctccgt gaaaagggtg ttgatgtcaa gggttatatg gaaaagatgg gttacgagat
     7201 tcctgagtac ggtattgaga gcttgccaat gggtggtggt attgaagtca cagttgccca
     7261 gcataccaat ggctatcaga ccttagctaa taatggagtt tatcatcaga agcatgtgat
     7321 ttcaaagatt gaagcagcag atggtagagt ggtgtatgag tatcaggata aaccggttca
     7381 agtctattca aaagctactg cgacgattat gcagggattg ctacgagaag ttctatcctc
     7441 tcgtgtgaca acaaccttca agtctaacct gacttcttta aatcctactc tggctaatgc
     7501 agattggatt gggaagactg gtacaaccaa ccaagacgaa aatatgtggc tcatgctttc
     7561 gacacctaga ttaaccctag gtggctggat tgggcatgat gataatcatt cattgtcacg
     7621 tagagcaggt tattctaata actctaatta catggctcat ctagtaaatg cgattcagca
     7681 agcttcccca agcatttggg ggaacgagcg ctttgcttta gatcctagtg tagtgaaatc
     7741 ggaagtcttg aaatcaacag gtcaaaaacc agggaaggtt tctgttgaag gaaaagaagt
     7801 agaggtcaca ggttcgactg ttaccagcta ttgggctaat aagtcaggag cgccagcgac
     7861 aagttatcgc tttgctattg gtggaagtga tgcggattat cagaatgctt ggtctagtat
     7921 tgtggggagt ctaccaactc catccagctc cagcagttca agtagtagtt ctagcgatag
     7981 cagtaactca agtactacac gaccttcttc aagggcgaga cgataattct ctaaatgaag
     8041 tggccaatca atggattgcc acttttttcc tttcgtgtta caataagggt atgaatcagt
     8101 atcagaaaaa gattgttaac ggaaaaattt attcgctcct atccggctta atatggggaa
     8161 tctgtggaat tttaggagag tacttcttta ctcattatca ggtgtcttcg ggctggatta
     8221 cctctatgcg tttgacactg gcagggagtc ttgtactcat ttggtctgca atacaattaa
     8281 aatcgcaagt gctagatatt tggcgagaca agaaaaatta cctgcccttt ttagcctatg
     8341 ctattttggg gattttttca gttcagtatt ttttctatct ctgtgtagaa tactcaaatg
     8401 ctacgacagc aactatttta cagtttatta gccctgtctt tatcctcttt tataatcgct
     8461 tggtttatca aaaacgagcg tcaaaaagcg ctgttttcta tgttttggtt gccatgctgg
     8521 gtgtttgctt gatggcgaca aagggagatc tctctcagtt atccatgacg ccgctagctc
     8581 ttataacagg tttgctgagt gccatgggtg ttatgtttaa tgttatcttg ccccaacctt
     8641 ttgctaagcg ttatggtttt gttcctacgg ttgggtgggg gatgattttg gcaggtttgt
     8701 ttagcaatgt cctctcgccg gtttatcagc tttcctttac tcttgatatt tggagtatct
     8761 tgatttgcct cattatcgct ttctttggaa cggcttttgc ttttttcatt tccatgaagg
     8821 ctgtgtcctt ggtttctcct ttggtggttg ccgttatcag tgccagtgaa cctctctctt
     8881 ctgctctctt gagtgttttg ttcttaggat tagtagtgga ttggtccctc cttctagcta
     8941 tagccttgat tattttaccc atgatatttt tgtctataga agaagcgaaa gaaagtagat
     9001 aaaaagtctt ttcttaattc taaaagtgtg ctatactaga atagtcaata aaacacgggg
     9061 agatagttgt gaagagtgtt tttaggttgt atcggtaggg gcttgctttt accgacagca
     9121 cgttttatct cacatcccta aaaaatcata cctaaaaaca gacaaaaagg tttcaaattt
     9181 gaggcctttt ttggtagaat aggtatcatt ataacgaacc aggaggcatc tatgactgct
     9241 acaaaaatga acgctcaaga aattatccaa tttatcgcca atgctgaaaa gaaaaccagt
     9301 gtcaaagtaa cctttgaggg gcaactcgca actgctgtgc ctagctctgt tgtcaaacta
     9361 ggaaatgtcc tattcggaga ctggaaggac gtggctccgc ttcttgaagg tttggtagaa
     9421 aatcaagatt atgttgtcga gcaagatgct cgtaattctg cagttccttt gctagataag
     9481 cgtgctatca acgctcgtat cgagccaggt gctattatcc gtgaccaggt ggaaattggt
     9541 gacaatgctg ttatcatgat gggagctgtt atcaatatcg gtgctgaaat cggtgctgga
     9601 accatgattg acatgggtgc catccttggt ggccgtgcta tcgttggaaa aaatagccac
     9661 gttggtgcag gtgcagtttt ggcaggtgtg attgagccag ctagtgctga accagtccgt
     9721 gtcggagaca atgttcttat cggtgctaat gcagtggtta tcgaaggagt ccaaatcggt
     9781 agtggttcag ttgtcgctgc aggagctatc gttacccaag atgtcccaga aaacgtggta
     9841 gtagcaggtg ttccagctcg tatcatcaaa gaaattgatg cccaaaccca acaaaaaaca
     9901 gcgctagagg atgcgcttcg taccttgtaa ttgtaaaagt aaaaaagagg cggaaccctt
     9961 tttccagcct ctttctgcta tatcggagga cagatagatg ttagatttga ttcagactag
    10021 acgagcttta caccagattc cagagattgg cttggaggag ttcaagactc aggcttattt
    10081 gctggatgtg attgagaaat tgactacggg caaggatttt gttcaaattc gtacttggcg
    10141 gacagggatt ttggtctacc tgcagggaag tcagccggag cgaaccattg gttggcggac
    10201 agatattgat ggcctgccta tcgtcgaaca aacaggtcta ccttttgctt ctcaacacca
    10261 aggtcgcatg catgcctgtg gccatgattt tcatatgacc attgccttag gctgtcttga
    10321 gcgcgccctt gaggagcaac ccaagaataa tctgctcttt ctatttcagc ctgctgaaga
    10381 aaatgaagct ggtggtatgc tcatgtatga ggatggtgct tttggagatt ggttgccaaa
    10441 ccaattctat ggtctccatg ttcgtccaga tttgaaagtc ggccagattg cgactaatac
    10501 tcatacactc tttgcaggaa cttgcgaggt gaagatccgt ttcaaaggca aaggtggcca
    10561 cgcagctttt cctcatgaag ccaatgacgc cttggtggcg gctagttact ttgtgaccca
    10621 agtgcagtca gttgttagcc gcaatgtcaa cccaatcgag ggagcagtgg tgaccttcgg
    10681 cgtttttcaa gctggaacca ccaacaatgt catcacagac acagcttttt tacatggaac
    10741 tattcgtgcc ttgacacagg acatgagtct cttggtgcaa aagagggtaa aaacagttgc
    10801 agaaggggtt gcagctgcct ttgatatgga agtcgaagtg gaactcaaac aaggtggata
    10861 cctacctgtt gagaacaatc cagccttggc gcgtgaactg atggacttct ttgacgaaaa
    10921 agacggaatc gagttgattg atatcgaacc tgctatgaca ggcgaggact ttggttatct
    10981 cctttcaaag gttgatggcg tgatgttttg gctaggtatc gatagtccct acgctcttca
    11041 tcaccctcaa atgagtccta aggaagaagt cttagctatt ggggtggctg cggtgtctag
    11101 tttcctgaaa aagaaggcag cagaatagag ggcttgtcta tgaaatcgga attacgcaag
    11161 caagtcttgc aaggaatgaa ggctatatct caagaacaaa aacagtttat agatcaaact
    11221 ttaaccgagc ggcttttaca ccatcccttc tatcaagaag ccaaggtcat cgcaacctac
    11281 ctctcttttt ctcatgagtt tcaaacgcgg gaactgattg agcaggcgct gaaggacggc
    11341 aagaaggttt taatacccaa aacttatccc aaggggcgca tggactttgt agtctatgat
    11401 ctgcagcagt tggtaaaaac tgcctttgcc ttactggagc cacagggaga tttggaagtg
    11461 gtggatgtat ctcagattga tttgattcat gttcctggcc tggcttttac gacggatgga
    11521 tatcggattg gatatggtgg aggttattat gaccgctatc tggaacattt ttctggtcat
    11581 actttgagta cggttcatcc ttgtcaaatt caggacttta tacctgaaaa ccatgatatt
    11641 cctgttcagg aggtattaat tgatgaagga aatctttgat agacgttacc ctgtgacgag
    11701 tttcttcctc ttagtgacgg ccttggtatt tttactaatg ttggtcactg caggcgggaa
    11761 ctttgacagg gcagatacat tatttcgatt tggagccatg tatgggccag ctattcgcct
    11821 ctttcccgag caggtttggc gtctcttgtc tgccattttt gttcatattg ggtgggaaca
    11881 tttcattgtt aatatgcttt cactttatta tcttggaagg caggtagagg agattttcgg
    11941 ttctaagcag tttttctttc tctatctttt atcaggaatg atgggcaatc tctttgtttt
    12001 tgtatttagt cctaaatcct tagcagcagg cgcctctacc tctctttatg ggctatttgc
    12061 cgcgattatt gttcttcgct atgcaactcg caatccttat attcaacagc tagggcaatc
    12121 ctatctgaca ctttttgtgg ttaacattat tggaagtgtt ctgattccag gaatcagcct
    12181 agcaggccat atcggtggtg cagttggtgg cgcatttcta gcagttatct ttccagttag
    12241 aggagaaaaa cggatgtata ataccagcca gagattagga gcggtagtct tgttcgtagg
    12301 actcgccatt ttgcttttct acaagggaat gggaatgtga cgaagttgaa agaaaatttg
    12361 tgtaatgaaa aaagataagg cattttttga accttatctt tttattttat tccttctcag
    12421 tcaattcttt tccaagttgg atgaggtaat tcttcaaatc atctttgact tgtggatgtt
    12481 tgagggcgta gtcaatagat gttttcataa agccaaactt atccccaacg tcgtaacgag
    12541 ctcctgtaaa ttcacgggca aaaacacgct gtgttttatt aagagtatcg attgcatcag
    12601 taagttggat ttcatttcca gcaccaggag cttgcttttc gagaatctca aaaatttcag
    12661 gcgtgaggag gtagcgtccg ataatagcaa ggtcgctagg agcgtcctct ggagctggtt
    12721 tttcaacaaa ggtttcaaca ctgtaaagac catcttttcc ttcgccttgc ggagcaataa
    12781 ccccgtaagc agatacttcg tcatgaggga ctggcatgac agcgatagta gacgcgtggg
    12841 tacgctcgta gtcatccatg agttgtttgg taagtggaac agtcttttcg tctgtgatat
    12901 ccatcaagtc atcacccagc ataacaacaa aaggttcatt tccaacgaaa gccttggctt
    12961 gcaaaacagc atcgccgaga ccacgtggat gcgtttggcg gataaaatgc aggcgcatgc
    13021 cagttgtttc atcaactagc ttcagaagat ctgttttccc tttttctttg agattatatt
    13081 ccaattcaaa gtttgaatca aagtggtcct caatagaacg ttttgattta ccagtgacaa
    13141 ctagaatatc ttcaatacct gatttgagag cttcttccac gataaactgg atagttggtt
    13201 tgtctacgat tggcaacatt tctttggcaa gggccttggt tgctggtaaa aatcgagttc
    13261 ctagtccagc agcagggatg actgcctttc taacttttga tgtcataaaa atcctttcta
    13321 tagagggtta agaccactca ttttctgctt taaattcatt gttcatgatg tcataaatgg
    13381 catctttgat attggttccg tgataaataa cttggtaaat agcctgtgta atgggcatat
    13441 agactccaag ttcttgggct agttcatagg ctgctcgagt cgttgaaatt ccttcgatta
    13501 ccatgcccat attagcttct atatcagcta gggattctcc tcgtccgaga gcatctccag
    13561 ctctccagtt acgagagtgg atggaagttc ccgttacgat caaatctccc acaccagata
    13621 agccgctata ggtcaatgga ctggccccga gtgctacccc taggcgggtg atttctgcta
    13681 aacctcgagc gatgatggct gccttagcat tatcaccaaa tccaagacca tgtaaagctc
    13741 cagcaccgac agcaataata tttttaagag caccagcagt ttcaaccccg ataacatccg
    13801 tattggtata aagtcggaag tagtgattac taaatagctc ctgaacgtat tgagctgttt
    13861 gtaaatcttt agaagcagca gttattaaag ttaggtcacg cacaatggtc tcttctgcat
    13921 gactaggccc tgaaacaacg acgatatcac tacggagatg ttcaggaatt tcttcttcaa
    13981 gaatggttga taatcgttta tggctatcag gttctaatcc ctttgatgcg tgcatgatga
    14041 taaccttatg gtccaaggtt tgtgcaactt gctgggcaac aagtcgtgtc acttttgttg
    14101 ggacaacaaa caaaatcgca tccacatttt tcaatgtttc tgctaagtcg gtgtaggcaa
    14161 tgatattttc gtctagaacg acatctttaa agtagtgctt attagtatgg tgtgtattaa
    14221 tttcattgat ttgctcggga agatttcccc aaatacgtac ctcgtgtcca ttgtcattta
    14281 agacttgtga aagggcggtt ccccaagaac caggccccaa gacggcgacg gtttgttttt
    14341 ccatgatttc ctccttgata gatttctttc tcttattcta tcatattcta ggggattttg
    14401 cacttgcatt actggggtat ggtggaaaaa tacacaaaga ccgagatgaa gggataaact
    14461 cttagtcttt agttaaagtg agaagaaatc tgatagatag tttgattcag tatatgctta
    14521 taatagaggt aaacaactca ggaggttctt gtaggttgcg agagtttggc gaaaaaatta
    14581 aaagattacg tttggctaaa aaaatcagtc gttcagaatt ttgtggtgat gagtctgaat
    14641 taagtatccg tcaattaatt agaattgaaa atggagaatc cagaccaata ctaacaaagt
    14701 taaaatatat tgctgaacgt ttggaggttg aagattacaa gttgatgcca agttatatag
    14761 agttggataa ggaataccta gaattgaagt atttcttgat gaggactcct acatacgaag
    14821 atgaaactat cgcccaaaag aaagagagtg tttttgctaa gatttttgaa gagtattatg
    14881 ataggctacc tgaggaagaa agatttatca tcccaaatta ttcatatcta gcactagcga
    14941 actacacagt tcaaaaatta ccagaaaagc tagttgaaat actgtccttc tggtgatttt
    15001 gcttaatttc tgatgttaga gacataattg aggagatttg tcttgatttt ttcctaaaaa
    15061 agggcagact tctcccttgg tttgatgctc gatttttaaa ataaggtgac ttattctgtt
    15121 aggcatgttt tcccctgccc tctagctcta tatggaacag ggagtatcag atttatctgg
    15181 cagattgtat caaataaggc ttgaaactgt tttgaatagg cgtatagact tgagaatttg
    15241 agaatatgtc gtctagcagt agagacatat tttccggcaa tatgaaggaa gagacgtcga
    15301 aaacgcttga tagtcaaggc ctttacttca tcaccagcta gctgctttaa aaagaggtag
    15361 agattataag ccagacagcc catcatcata cgtacttcat tcttaatcat ggtcgaacta
    15421 tctgtcttat ccccaaagaa tcctgctttc ctttctttga taaagttttc agcattccct
    15481 cgttcacgat agaattgaaa cagctcctca ctatctacac tgcttaaatt ggtcaaatga
    15541 acttttttac aaccataaaa aagagcactt aatcgtactc ttctctttta aggattagtt
    15601 gaattaattc aaatccacta gttctcctgt ttctaggtag actaggcgtt cacaaatgtt
    15661 agcaatgtaa tcagcgaagc gttccagatg ccctatgata taaaggtatt gtgttccatt
    15721 gggaattgag gtttcttggt ctttcataag tccaatgatt tcctttgata aggcataata
    15781 atattggtca atctgttcat ctttttgagc aatactaata gcttttgagg cttggtgcaa
    15841 aggaaaggca accaataaat cggatagcat gctgagggat aatttaccca tttggtgtaa
    15901 ctgttcttcg tcaggggcta gttgattttc ttttagttgc aaaacagctt tagcaatgcc
    15961 tgccatatgg tctcccatac gttcaaggtc tgaacaagaa gacatgatgc taatcacaaa
    16021 tcgaaggtca gacacttgtg gctgctgcaa ggccaacaaa cgggcacagg tcaattcgat
    16081 agcgctttga ccttggttga tagcatgatc cttattgata attagctctg ccatctcctt
    16141 gtctttggag gctaaggcca gtaaggtttt tgaagctgtt tcaaggacaa gttgccctag
    16201 tcctaaaaag gattgttcta attcatgcaa ttctaagtca aattgatttc tcataggttt
    16261 ttccttcctt atccgaaccg tcctgaaata tagtcttctg tgcgctgatc ttttggattg
    16321 gtaaacacgt caacggtgtc tccaaattcg caaatttctc ctgttaagaa aaaagcagtt
    16381 ttatctgaaa tacgtgaagc ttgttgcatg ttatgggtaa caatgataat cgtataatcc
    16441 ttttttagtt gctgaatgag gtcttcaatt tttaaagtgg agatagggtc taaggctgaa
    16501 gtcggctcat ccattaacag aacatcaggt tctactgcta aagctcgcgc aatgcaaagg
    16561 cgttgctgct gaccgcctga taaggacatg gcactctttt taagattatc tttgacttcg
    16621 tcccaaatgg ctgccccttt taaagatttt tccactaagg catctaattg ttttttgtct
    16681 cgaataccat gtgtccttgg gccataagcc acgttatcat agatagacat ggcaaaggga
    16741 ttaggctgtt gaaaaaccat ccctacacgc ttgcgtagct gattaaggtt gaatttgcta
    16801 ctataaatat cttgctcatc taagaggact tggccttcaa tatggcaaga aggaaccaaa
    16861 tcgttcatcc gattaagggt ttttagaaaa gttgatttac cacaaccaga tgggcctatc
    16921 aaggcagtaa tctgtctttc tggtaattga atcgaaatat tttttaaggc ttgaaaatcc
    16981 ccgtaaaata agtctaggtg tctgactgaa aatgttccca tactaggaag ctcctttcac
    17041 aagttttcga gataataagc ttgatagagt atttatcatt aaaacagtaa taatcaaaat
    17101 cacaccggta gcataggctt cattgacatg tagcccctca cttgacagca tatacatatg
    17161 cagggctaga gaacgacctg aagacatgag actacttggc gtattggtag aggtacctaa
    17221 tgtatacatg agggcagctg tctcaccaac gatacggcca atagctagta tcactccagc
    17281 taaaatacct ggcatggcaa ctggtagaac aattctaaaa acagtccgta acttacctgc
    17341 cccaagtcca taacttgctt gacgcatgct atcactaaca gataaaaggg cttcttctgt
    17401 tgagcgaata atgactggca acaccatgat gactgaggtt aagattcctg ataacagaga
    17461 gtattgaaaa cctaagaaga ctacaaagaa gagcatgcca aacagaccaa aaacaatgga
    17521 aggaatccca gacaaggtat ctgaggccaa tcgcataatt ttaacacaaa gggaatcttt
    17581 ttttgtatat tccacaagat aaaaaccagc aaaaatccct atgggcaagg ctaaaagaag
    17641 agcaccaaaa accagaataa cggtggaaat aatcgctggc ataagggaaa tgttctcaga
    17701 agtataagtc caagaaaaga gggatagact tagatgaggt aagcctttga tgaggataaa
    17761 accaatgatt aaaaagagag agccaaaggt taaagctgaa aaacaataaa cgagaagttt
    17821 tagcaggtat ttactcataa gatgattttc ctttcaagta ggcaaagtag gcattaatca
    17881 agagaataag gaaaaagaga actgctgagg ttgcaataag ggcttcccta tgctgacctg
    17941 atgcgtaagc catttccaga acaatattgg ttgttaaggt tctggttcct gaaaagagtc
    18001 cacttggaat aatcggctgg ttgcctgcca ccaaaataac tgccatggtt tcacctactg
    18061 cgcgaccgat tcctaaaata actgctgata aaataccaga tctcgcagct ggcaagatga
    18121 cactaaaaat actccgttca tgactagctc ctagagccaa gctaccagaa taatacgttt
    18181 tgggaactgt tcggatagca gattctgaca aactgataat ggttggcaaa atcattattc
    18241 ctaatagtaa cgaagcggtt aggacactca tgccatttcc taaaaaggtt ctaatccaag
    18301 gcaccaataa ttgtaggccg aaaaaaccat aaacaataga tggaatggcc gccatcaagt
    18361 tgatagctga ttttaagaag ccatagacgg gctttggaca ataataaacc ataaacaccg
    18421 atgtcaagat gcctgttggc accccaatca caatcgctcc taaggtaatt aataaggaac
    18481 caacgatcat tggtaaaata ccatagcttg ccggaatgtt cgttggcgac caatcactgc
    18541 ctaataaaaa acgggcaaag ccgtagttag ccatgaaagg taagccatta ctaaaaataa
    18601 agaaacagat tagcaaaata gctacaacag ctactgttgc actcatgaaa aaaattgccc
    18661 taaaaactgc ttctttgaag gcttgtttta tcacatcttg tcctttctag tgaagaaagt
    18721 aagggagata cgacacctcc ctacttgcct tctttatctt attgtacgat gaaacgtctg
    18781 catctcttta gagatttatg gagcaaacat tttatttaat cttgtcccag gtggttaatt
    18841 tgccactaaa aacgtctgca agttcagcca tactgacttg gcttgcctta ttgtcattat
    18901 tgaccacaac agcaataccg tctaaagcaa tagcatcatg ggtgagactc ttaccttctt
    18961 caggagttaa ttccctagaa accataccaa tatcagcggt tttctcctta acagcggtaa
    19021 tacctgctga agatccatta gaggtaatat caatcgtaac ttctggattt tcttttttat
    19081 aagcttctgc taatttttcc attaaagaag atactgaagt ggaacctaca acagacaact
    19141 tgcctgataa gtgttggctt gtatattctg tggtttcggt tttagcttca ataaatttat
    19201 tatctgtgac cacttgttga ccttgtttgg agtggataaa gctgataaaa tcttgaccta
    19261 gcttggaaag attagaagac caaacaatgt tgaagggacg ttgaagaggg tattcaccat
    19321 ctaaaactgt gtctcgacta gccttgacac catcaatctc taaagccttg acagatttcg
    19381 ttaaagatcc caaggagatg tagccgatag cattagcatt cccttgaact gctgagagaa
    19441 caccttctgt actattttga atcacagctg ttttggtagt gttgtcaatt tttttatcac
    19501 cgtctttttt gagaatccct gtgatttctg tgaaggcacc ccgtgttcca gagccatttt
    19561 ctcgtgaaat cacctcaatc gttcctgaag ctgactgttt ggaagcagct gactgattgc
    19621 cacaggcaac aagcccaaat cctgataagc caatggctgc aagagtaagc atttttttga
    19681 atttcataat aatcaccttt atctctatgt atttttcttg tgtaggctta ctacatttat
    19741 agtctaacaa gtctttgtaa aggtttatcc ctgattcatg taaagattgt gtaaagaatc
    19801 aaaaaaagcc acttttgaaa aatggctgct cctaaaaata gctttaaaaa ttattagtcc
    19861 tgtgcgaaag attggttagg aagaaaaatc gtgaagcaac tgcctctgcc aagctgactc
    19921 gtcaccgtga cttggccacc taataattga ctgagttctt tgacaatggc aaggccaaga
    19981 ccagtgccac cagtttgtct gcttcgacct ttattaactc ggtaaaaacg ttcaaaaata
    20041 cgatcctgct ctaattgact aataccaatc cctgtatctg atacagaaat cttaatgcct
    20101 tcgttcacct tttgggtctt gacctcaatt tttccccctt gttcagtgta acggatggca
    20161 ttggataaaa gattgagtaa gatttgggaa agtaattgac tatctgatac gagggtgaca
    20221 tcatctggca cctgcacctt tagctgtaaa tccttcttct tgagctgagg ttgcaagctt
    20281 tgagtcaaat cctgtacaaa ttctgccaaa gaaagggtcg tccattgtat aggcatttgt
    20341 tgagccttag ataaggtaag aagatgctca acaatatgct caagacgcaa actttctttg
    20401 taaataatgt ctagaaagtc atccttgagc gcttcttctt cagctgacat ccccttaatg
    20461 gtttcagcaa agcccttaat cgaagtaact ggtgtcctca attcatggga ggcatttgag
    20521 acaaaggcta aatttaactt ttcataagtt ctaatcgttg ttaaatcata tagcaagacg
    20581 agcacagctt ccacagattg ggtggggcta aaaacgggaa ctgctgtcac ttctaaaatc
    20641 aagtcaccct catgaaaccc acttacttct tgttttaacc ttgttttttg atcaaaggct
    20701 tggtgaacta aattccgaat atccatccgt ttgaggtcat caagtgaact tatgtcgccg
    20761 tccacatcgg gaaaataatg aggcagagag cgactggata ataacatctg accttgagcg
    20821 gaaactaaaa acgtccccat ggttaggtgc gacagaagaa cctccattgt ttcggctaga
    20881 tccttgtatt gctgatcctg ttgggagact ttggttttta ggccagacac atactgagcc
    20941 aaagacttta agtcttcttg ccctttttct aaaaagtatt cactactggt caagagaggt
    21001 tggtgcaagg tctcaaaagc aacttcccat ttccaaaggc aaaagagcca gtagccacct
    21061 agtcccaaag aaagggctag aagaaagaga ccgatgcctt tactgatcca agttaatgcc
    21121 atccctgcaa tcagaatgag gctaacactt agattgacta gccaaaattg aaggtagcgt
    21181 ttcatctata actccttgaa cttataacca tacccccgaa tggttcgaat aaattgaggg
    21241 gctttaggat tgtcttcaat tttttccctt aacttaccaa tatgaacgtc caccaaacgt
    21301 gtttcctgcc caaagtcata cccccagata cgttccaaaa gacgctctct agtcagtgtc
    21361 atgttgggat gtttcataag atagagcaag agttcaaatt cttttggggt caaactcagt
    21421 aacttattcg ccttgtagac ttcatgacgc tcagggtata ctttcaaggt cccaaatagc
    21481 caagaatcgt cagcgatatt atctgaatca tctccttctt gttctccttt agttcgcctg
    21541 aggacagcct tgacacgcgc cagcaattct ctagggctaa aaggcttggt caggtagtca
    21601 tcagccccta attccaaggc caaaacctta tcaaattcat cacttttcgc agaaaccatc
    21661 ataattggag ttttgacgcc tttggctctc agccgcttac aaacttccat gccatctaat
    21721 tgtggtaaca tgatatcaag caagataaaa tcaaagggtt ctgtttctgc caaagctaag
    21781 gccttccgtc catttgttac caattgagta gaaaagcctt ccttacttaa atggtagtca
    21841 agcaatttca gaatgtgttc ttcatcatcc actaataaga cttgttttgt catctattat
    21901 ctcctattgg taacattata acacaattat cagaaatcct aacattgcta aatcagatta
    21961 aatttgctta tcaagactag tatctggtca aacgctcaat catctccttg tgctctggat
    22021 aggtcgccag tagatctacc ctttcaaata attcaaaatc ctcaaattca aaaccaggag
    22081 caacaagaca agaaaccaga gcatcatcct tatcaactgt tgatccccaa atagtgccct
    22141 taggaacaca gtagtgaagt tgttgccctt tggatatgtc caggcctaaa gtgactgctt
    22201 cgtagtgacc atctgctgta atcatgtgaa cagtaagtgg ggattctgca tgaaaatacc
    22261 agatttcatc tgctgtcaat cggtgaaaat gtgaaggatt cgtttcttct taataagaaa
    22321 taaatactgg tataaagcgc ccttccctta ccagcaaggt ttatagtgtc tgaagctttt
    22381 tttgtttgtc taaaatagcc accttcaaca tggggagcta actctagagt tcttatcaag
    22441 tcttctttat ccgtcggagc caatgggttg aagtaactct tgttcaaagt ggtgttacga
    22501 tttcaagaac tcctctcagt tctgaggaca cggtaatgat tgatgcgacg gaagtacaaa
    22561 tcaatcgccc taaaagaaga attagcgaat gattctggta aaaaaaatgc cacgctatga
    22621 aggctcaagc gattgtcaca agtcaaggga gaattgtttc tttggatatc gctgtgaact
    22681 attgtcatga tatgaagttg ttcaaaatga gtcgcagaaa tatcggacaa gctggtaaaa
    22741 tcttggctga cagtggttat caagggctca tgaagatata tcctcaagca caaactccac
    22801 gtaaatccag caaactcaag ccactaacag ttgaagataa agcctataac catgcgctat
    22861 ccaaggagag aagcaaggtt gagaacatct ttgccaaagt aaaaacgttt aaaatgtttt
    22921 caacaaccta tcgaaatcat cgtaaacgct tcggattacg aatgaatttg attgctggta
    22981 ttatcaatca tgaactagga ttctagtttt gcaggaagtc tattatttgg ttaggtgaat
    23041 tagtgaagtg tttaggcaag tgtctctggt tacgacgtca tggcctctaa atcgattata
    23101 tttaggggtc atgactagtg aagcagttag ctagttcgca tataagcggc tagcgtctaa
    23161 caattaggaa ctttagttcc aataacttta agattacgac gttttaggac ataaatcgat
    23221 catatttatg tcctaaaact agtgaagcgc ctagccaaag tccgaatagg atttggcgtt
    23281 agttacttag attgctttgc aatcaagtaa ctttggcgat ttacatcttc tctggcgctt
    23341 ctactccaag caagcgaagg gcttctttga gaacgactgc ggttgcgtag ctgagggcta
    23401 gacggctgtc gcgttctggg ctttcatcca agatacgtgt atgtgcatag tatttgttaa
    23461 aggattgagc caggctaatt gcaaatttag caatgataga aggttcaaag ttatctgccg
    23521 cacggttgat aatacgtggg aagtcttgaa tgagtttaat gatttcccag ctttcagtat
    23581 cattcaagct atagttgcca gctgtttctg gtttgaaatc ggctttgcgt aagatagatt
    23641 ggatacgagc gtaggcatat tgaacgtaag gtccagtttc accctcgaag gataccatag
    23701 cttctaggtc gaagtcgtat ccatttgtac ggtcggtttt gaggtcatag aatttaatgg
    23761 ctccaacccc aacagcatgt gctacttggt ctttgttttc tagttcagga tttttagcct
    23821 cgatttggac cttggcacgg ctaacagcct ctgcaacagt aggctctagc aagatgacat
    23881 tccctttacg agtagagagt ttcttccctt cttttgtaac caaaccaaaa ggaacgtgag
    23941 taatgtcgtc actccagtcg tagcccatct cttgcaagac agctttgagc tgtttaaagt
    24001 gggcagattg ttcttgacca acgacataga tagatttagc aaattggtat tcgtttttac
    24061 ggtaaagggc tgcagccaag tcacgtgtga tatagagagt tgcaccatca gatttcttga
    24121 tgagggctgg atgttcaatt ccgtatttct caagattgac aacttgggca ccttctgatt
    24181 caagaagtag tcctttttca gaaagaatgt ctacaactgc atccatctta tcattgtaga
    24241 aggcttctcc gttatagctg tcaaattcaa ccttcaattc attgtaaagg cggttaaatt
    24301 ccactaaact ttcatcgcgg aaccattgcc aaagagcgag agcttcctca tctccatttt
    24361 caagtttacg gaaccattcg cgcgcttctt catccaagct agggtcattt tcagcttcag
    24421 cgttgatgcg gacatagagt ttaaggagtt catcgattgg atgagctttt acagcttctt
    24481 cgtcgcccca ttttttgtag gcaacaatca acatcccaaa ttgtttaccc cagtctccca
    24541 aatggttgac cttgaccgtt tgataaccga ttttttggaa aatatgtgac aagctatctc
    24601 cgataacagt tgaacgcagg tggccaatag aaaatggttt agcgatattc ggactagaca
    24661 tgtcgataac aacattttct tgtttgccaa tattttggtc agcatagtgt tctttttcag
    24721 tggtaacagc ttgcaatact tgagcagaaa tggcagattt atcaaggaaa aagttaacgt
    24781 aaggtcctgt tgcgacaact ttttcaaagg cttggctgtt cattttttca gccagttcag
    24841 ctgcaatcat ttgtggtgct ttacgttcga cttttgcaag agaaaaagca gggaaagcaa
    24901 tgtctcccat ttctgagttt ttaggggttt ccagtaactt taaaatagcc tcttggtcca
    24961 ggctatcaat gatgctagat aattcgctag caatcaattc ttttgtattc attaagagct
    25021 cctttttgga cttttctact attttatcac aattttaaag aaagaagaaa aaatttttga
    25081 aatctcctgt ttttttggta taatatggtt ataaatatag ttataaatat gcacgcaaga
    25141 ggattttatg agaaaaagag atcgtcatca gttaataaaa aaaatgatta ctgaggagaa
    25201 attaagtaca caaaaagaaa ttcaagatcg gttggaggcg cacaatgttt gtgtgacgca
    25261 gacaaccttg tctcgtgatt tgcgcgaaat cggcttgacc aaggtcaaga aaaatgatat
    25321 ggtgtattat gtactagtaa atgagacaga aaagattgat ttggtggaat ttttgtctca
    25381 tcatttagaa ggtgttgcaa gagcagagtt taccttggtg cttcatacca aattgggaga
    25441 agcctctgtt ttggcaaata ttgtagatgt aaacaaggat gaatggattt taggaacagt
    25501 tgctggtgcc aataccttat tggttatttg tcgagatcag cacgttgcca aactcatgga
    25561 agatcgtttg ctagatttga tgaaagataa gtaaggtctt gggagttgct ctcaagactt
    25621 atttttgaaa aggagagaca gaaaatggcg atagaaaagc tatcacccgg catgcaacag
    25681 tatgtggata ttaaaaagca atatccagat gcttttttgc tctttcggat gggtgatttt
    25741 tatgaattat tttatgagga tgcggtcaat gctgcgcaga ttctggaaat ttccttaacg
    25801 agtcgcaaca agaatgccga caatccgatc cctatggcgg gtgttcccta tcattctgcc
    25861 caacagtata tcgatgtctt gattgagcag ggttataagg tggctatcgc agagcagatg
    25921 gaaaatccta aacaagcagt tggggttgtt aaacgagagg ttgttcaggt cattacgcca
    25981 gggacagtgg tcgatagcag taagccggac agtcagaata attttttggt tgccatagac
    26041 cgcgaaggca atcaatttgg cctagcttat atggatttgg tgacgggtga cttttatgtg
    26101 acaggtcttt tggatttcac gctggtttgt ggggaaatcc gtaacctcaa ggctcgagaa
    26161 gtggtgttgg gttatgactt gtctgaggaa gaagaacaaa tcctcagccg ccagatgaat
    26221 ctggtactct cttatgaaaa agaaagcttt gaagaccttc atttattgga tttgcgattg
    26281 gcaacggtgg agcaaacggc atctagtaag ctgctccagt atgttcatcg gactcagatg
    26341 agggaattga accacctcaa acctgttatc cgatacgaaa ttaaggattt cttgcagatg
    26401 gattatgcga ccaaggctag tctggatttg gttgagaatg ctcgctcagg taagaaacaa
    26461 ggcagtcttt tctggctttt ggatgaaacc aaaacggcta tggggatgcg tctcttgcgt
    26521 tcttggattc atcgcccctt gattgataag gaacgaatcg tccaacgtca agaagtagtg
    26581 caggtctttc tcgaccattt ctttgagcgt agtgacttga cagacagtct caagggtgtt
    26641 tatgacattg agcgcttggc tagtcgtgtt tcttttggca aaaccaatcc aaaggatctc
    26701 ttgcagttgg cgactacctt gtctagtgtg ccacggattc gtgcgatttt agaagggatg
    26761 gagcaaccta ctctagccta tctcatcgca caactggatg caatccctga gttggagagt
    26821 ttgattagcg cagcgattgc tcctgaagct cctcatgtga ttacagatgg gggaattatc
    26881 cggactggat ttgatgagac tttagacaag tatcgttgcg ttctcagaga agggactagc
    26941 tggattgctg agattgaggc taaggagcga gaaaactctg gtatcagcac gctcaagatt
    27001 gactacaata aaaaggatgg ctactatttt catgtgacca attcgcaact aggaaatgtg
    27061 ccagcccact ttttccgcaa ggcgacgctg aaaaactcag aacgctttgg aaccgaagaa
    27121 ttagcccgta tcgagggaga tatgcttgag gcgcgtgaga agtcagccaa cctcgaatac
    27181 gaaatattta tgcgcattcg tgaagaggtc ggcaagtaca tccagcgttt acaagctcta
    27241 gcccaaggaa ttgcgacggt tgatgtctta cagagtctgg cggttgtggc tgaaacccag
    27301 catttgattc gacctgagtt tggtgacgat tcacaaattg atatccggaa agggcgccat
    27361 gctgtcgttg aaaaggttat gggggctcag acctatattc caaatacgat tcagatggca
    27421 gaagatacca gtattcaact gattacaggg ccaaacatga gtgggaagtc tacctatatg
    27481 cgtcagttag ccatgacggc ggttatggcc cagctgggtt cctatgttcc tgctgaaagc
    27541 gcccatttac cgatttttga tgcgattttt acccgtatcg gagcagcaga tgacttggtt
    27601 tcgggtcagt cgacctttat ggtggagatg atggaggcca ataatgccat ttcgcatgcg
    27661 accaagaact ctctcattct ctttgatgaa ttgggacgtg gaactgcaac ttatgacggg
    27721 atggctcttg ctcagtccat catcgaatat atccatgagc acatcggagc taagaccctc
    27781 tttgcgaccc actaccatga gttgactagt ctggagtcta gtttacaaca cttggtcaat
    27841 gtccacgtgg caactctgga gcaggatggg caggttacct tccttcacaa gattgaaccg
    27901 ggaccagctg ataaatccta cggtatccat gttgccaaga ttgctggctt gccagcagac
    27961 cttttagcaa gggcggataa gattttgact cagctagaga atcaaggaac agagagtcct
    28021 cctcccatga gacaaactag tgctgtcact gaacagattt cactctttga tagggcagaa
    28081 gagcatccta tcctagcaga attagctaaa ctggatgtgt ataatatgac acctatgcag
    28141 gttatgaatg tcttagtaga gttaaaacag aaactataaa accaagactc actagttaat
    28201 ctagctgtat caaggagact tctttgacaa ttctccactt ttttgctaga ataacatcac
    28261 acaaacagaa tgaaaaggag ctgacgcatt gtcgctccct tttgtctatt ttttaaggag
    28321 aaagtatgct gattcagaaa ataaaaacct acaagtggca ggccctggct tcgctcctga
    28381 tgacaggctt gatggttgct agttcacttc tgcaaccgcg ttatctgcag gaagtcttag
    28441 gcgccctcct tactgggaaa tatgaagcta tttatagtat cggggcttgg ttgattggtg
    28501 tggccgtagt cggtctagtt gctggtggac tcaatgttgt cctcgcagcc tatattgccc
    28561 aaggagtttc atccgacctt cgggaggatg ccttccgtaa aattcaaacc ttttcttatg
    28621 ctgatattga acaatttaat gcgggaaatc tagtcgttcg aatgacaaat gatatcaacc
    28681 agattcagaa cgttgtcatg atgaccttcc aaattctttt cagacttccc ctcttgttca
    28741 tcggttcgtt tatcctagcg gttcaaacct taccttctct gtggtgggtg attgttctca
    28801 tggtagtctt gatttttggt ttgactgctg tcatgatggg aatgatgggg cctcgttttg
    28861 ccaagtttca aacccttctt gagcgcatca atgccattgc caaggaaaat ttacgtggcg
    28921 ttcgtgtggt caagtccttt gtccaagaaa aagagcaatt tgctaagttt acagaggtct
    28981 cagacgagct tcttggtcaa aacctttaca ttggttatgc cttttcagta gtggaaccct
    29041 ttatgatgtt ggttggttac ggggcggtct tcctctctat ttggctggtc gcgggaatgg
    29101 ttcagtcgga tccgtctgtt gttggttcca tcgcttcttt tgttaattac ctaagccaga
    29161 ttatctttac cattgttatg gttggatttt tgggaaattc tgtcagccgt gccatgattt
    29221 ccatgcgtcg tattcgagaa attcttgacg cagagccagc tatgaccttc aaggatatcc
    29281 cagatgaaga gttggttgga agccttagct ttgaaaatgt gacctttacc tatccaatgg
    29341 acaaggaacc gatgctgaaa gatgtgagct ttactattga acctggtcaa atggttggtg
    29401 tagttggagc gactggtgca ggaaagtcaa ccttggctca attgattcca cgtctctttg
    29461 atccacagga aggggctatc aaaatcggag gtaaggatat tcgagaagtg agtgaaggga
    29521 ccctgcgtaa aacagtttcc atcgttctcc aacgtgccat tctttttagt ggaacgattg
    29581 cagataactt gagacagggg aagggaaatg ctactctatt tgaaatggag cgcgcagcca
    29641 atattgccca ggctagtgaa ttcattcatc gtatggagaa aacctttgaa agtccagttg
    29701 aagaacgggg aaccaatttc tctggtggac aaaaacaaag gatgtcgatt gcgcgtggga
    29761 ttgtcagcaa tccacgtatt ctgatttttg atgattcgac ctcagccttg gatgccaaat
    29821 cagagcgctt ggtgcaagaa gctttgaata aggacttgaa ggggacgaca actattatta
    29881 ttgctcaaaa aattagctcg gttgtccatg cagacaagat cttggttcta aatcaaggac
    29941 gattgattgg tcaaggcacg catgcagact tggttgccaa caatgccgtt taccgtgaaa
    30001 tctatgaaac acagaaatga aagacaaact ataagaaaag tcaatagttt tatctaaact
    30061 atttcttatt tcaatttgat gatttggcga tgattttaga gcacggcaaa aagcccttga
    30121 aaaagtccat tttttcaaag gtaatcctgt gttaatttca gaaattacat cactttttgt
    30181 tcgtcaaatg gcagctcttt tttaggatat aaaacagggt tcggataagt ttttttgcaa
    30241 ggtggatgat ggctacattg taatgttttc cttgttctaa cttagtctta agataggtct
    30301 taaaagcagg tgaaaagcga gggcatgctt tggcagcttg tatgagtacc taccgcagat
    30361 gaggggaacc ccgtttgacc attcttccag ctaaatcaat ctgacctgac tgataaatag
    30421 aagaatccag tccagcgaaa gcttgtaatt gagcaggatt atcaaaggca tgaatatttc
    30481 gaatctcggc taaaatgacc gcccctaaac gattctcaat cccagtaacc gtcgtgatga
    30541 ccgagttgaa ctcagccatc aagtcattga cacatgtttc cgccttgtca atgagcctct
    30601 tgtaatgttt gatgttttca ttacacgaga taaaacgtct atgcgttatc aaactcatta
    30661 ccaattaaaa caaaaagctg tggttagatc ctttcggaaa ttgtcaagcg attggaggaa
    30721 atgaactaat ccacagcggc ttattccaag tataccactt gggctttggc agtagctaac
    30781 tgcgctaaat ataatataag gaggagtaaa atgaagacag ttcaattttt ttggcattat
    30841 tttaaggtct acaagttctc atttgtagtt gtcatcctga tgattgttct ggcgactttt
    30901 gcccaagccc tctttccagt cttttctgga caagcggtga cgcagctagc caatttagtt
    30961 caagcttatc aaaatggcaa tccagaactt gtatggcaaa gcctatcagg aatcatggtc
    31021 aatcttggcc tgctggtttt ggttctattt atctctagtg taatatacat gtgtctcatg
    31081 acgcgcgtga ttgcagaatc gaccaacgag atgcgcaaag gcctctttgg taagcttgct
    31141 cagttgacgg tttctttctt tgaccgtcga caagatggcg atatcctgtc tcattttacc
    31201 agtgatttgg ataatatcct ccaagccttt aacgaaagct tgattcaggt catgagcaat
    31261 attgttttat acattggtct gattcttgtc atgttttcga gaaatgtgac gctggctctc
    31321 atcaccattg ccagcacccc attggctttc cttatgctga ttttcatcgt gaaaatggca
    31381 cgcaaataca ccaacctcca gcagaaagag gtagggaagc tcaacgccta tatggatgag
    31441 agcatctcag gccaaaaagc cgtgattgtg caaggaattc aagaggatat gatggcagga
    31501 tttcttgaac aaaatgagcg cgtgcgcaag gcaaccttta aaggaagaat gttctcagga
    31561 attcttttcc ctgtcatgaa tgggatgagc ctgattaata cagccatcgt catctttgct
    31621 ggttcggctg tacttttgaa tgataagtct attgaaacaa gtacagccct aggtttgatt
    31681 gttatgtttg cacaattttc acagcagtac taccagccta ttatccaagt tgcagcgagt
    31741 tggggaagcc ttcagttggc ctttactgga gctgaacgaa ttcaggaaat gtttgatgca
    31801 gaggaggaaa tccgacctga aaaggctcca accttcacta agttgcaaga aagtgttgaa
    31861 atcagtcata tcgatttttc atacttgcct gataaaccta ttttgaaaga tgtcagcatt
    31921 tctgccccta aaggccagat gacagcagtt gttgggccga caggttcagg aaaaacgact
    31981 attatgaacc tcatcaatcg cttttatgat gttgatgctg gtggtattta ttttgatggt
    32041 aaagacattc gtggctatga cttagatagt cttagaagca aggtgggaat tgtattgcaa
    32101 gattcggtct tgtttagcgg aacgattaga gacaatatcc gatttggtgt gccagatgct
    32161 agtcaggaaa tggttgaggt agcagcaaaa gcaacccaca ttcacgacta tatcgaaagt
    32221 ttgcctgata agtacgatac tcttattgat gatgaccaga gcatcttttc aacagggcag
    32281 aagcaattga tttcaatcgc tcgaaccctg atgacagatc cagaagttct cattctcgat
    32341 gaagcaactt caaacgtaga tacggtgaca gaaagcaaga ttcagcatgc catggaggtg
    32401 gttgtagcag gtagaactag tttcgtcatt gcccaccgct tgaaaaccat tctcaatgca
    32461 gatcagatta ttgtccttaa agatggagaa gtcattgaac gtggtaacca ccatgaactt
    32521 ttgaagctag gtggctttta ttcagaactc tatcacaatc aatttgtttt cgaataagaa
    32581 agaagttgtc ctatgtgggc agctttttct tgtccataaa aaatgtttat cacagcctta
    32641 aaaaaaacat attagacgaa agtcattttg agtgatatga taggactatc gttagtattc
    32701 gaaaggagag gcatcatggc tagaacggtt gtaggagttg ctgcaaatct atgtcccgta
    32761 gacgcagaag gcaaaatcat tcattcatct gtatcttgta gattcgcaga gatcattcgt
    32821 caagtcggtg gtctcccttt agtcattcct gttggtgatg agtcagttgt acgtgattat
    32881 gtggaaatga ttgacaaact cattttgaca ggaggccaaa atgttcatcc tcagttttat
    32941 ggagagaaaa agaccgtcga aagcgatgat tacaatctgg tccgtgacga atttgaattg
    33001 gcactcttga aggaagcgct tcgtcagaat aaaccaatta tggcaatctg tcgcggtgtc
    33061 caacttgtca atgttgcctt tggtggaacc ctcaatcaag aaatcgaagg tcactggcaa
    33121 ggactacctt tcgggacatc tcactctatt gaaacagtag aaggaagcgt ggtggctaag
    33181 ctatttggga aagaaagtca ggtcaactca gtccatcgtc aaagcattaa agatttggca
    33241 cctaatttcc gtgtaactgc tattgattca agagaccaaa ctattgaagc gattgagtct
    33301 atcgacgagc accgcattat cggtttacag tggcatccag agtttctggt taatgaagaa
    33361 gatggcaatt tagaattatt tgagtattta ttgaatgaac tgtaacgatt ggaacatctg
    33421 gtcgttcttt cttttatttt ttcaaaattt ttagaatgtg gcattttacg caaacgtttg
    33481 aattctgata aaaattggag aaattcgaca aaaaaacttg aaaaaaacga aggtaagcgt
    33541 tatgatagaa aagaagaaat attggaggaa gaacatgtca catattaaat ttgattattc
    33601 aaaagtttta gacaaatttg ttgcaccaca tgaagtggaa tacatgcaat cacaagtaac
    33661 agcagcagat gaattgatcc gtaaaggaac tggtgctggt agcgacttct tgggatggtt
    33721 ggaccttcct gaaaaatacg accgcgaaga atttgaccgc atcttgaaag ctgctgagca
    33781 aatcaagtca gacagcgatg ttttggttgt gatcggtatt ggtggatctt accttggtgc
    33841 aaaagcagca atcgacttct tgaatcacca ttttgctaac ttgcaaacaa aagaagaacg
    33901 caaagctcca caaatccttt acgcaggaaa ctcaatctca tctacttacc ttgctgactt
    33961 ggtagagtat gttgcagaca aagacttctc agtaaacgta atttctaaat caggtacaac
    34021 aactgaacca gcgattgctt tccgtgtctt taaagaactc ttggttaaga aatacggtca
    34081 agaagaagct aacaaacgta tctatgcaac aactgaccgc caaaagggtg ctgttaaggt
    34141 tgaagcagac gctaacggtt ggggaacatt tgttgttcca gatgatatcg gtggacgctt
    34201 ctcagtattg acagccgttg gtttgcttcc aatcgcagca tcaggagctg acatcaaagc
    34261 tcttatggaa ggtgcgaatg cagctcgcaa agactacact tcagacaaaa tctctgaaaa
    34321 cgaagcttac caatacgcag tcgttcgtaa catcctttat cgtaaaggct atgcaactga
    34381 gatcttggta aactatgagc catcacttca atacttctca gaatggtgga aacaattggc
    34441 tggtgaatca gaaggaaaag accaaaaagg tatctaccca acttcagcca acttctcaac
    34501 tgacttgcac tcacttggtc aatttatcca agaaggaact cgtatcatgt ttgaaacagt
    34561 tgtccgtgtt gacaaacctc gtaaaaacgt gcttattcct actttggaag aagaccttga
    34621 cggacttggt taccttcaag gaaaagacgt tgactttgta aacaaaaaag caactgacgg
    34681 tgttcttctt gcccacacag atggtgatgt accaaacatg tatgtgactc ttccagagca
    34741 agacgctttc actcttggtt acactatcta cttcttcgaa ttggcaattg ccctttcagg
    34801 ttacttgaat gctatcaacc catttgacca accaggtgtt gaagcttata aacgtaacat
    34861 gtttgccctt cttggaaaac caggatttga agaattgagc aaagaactta acgcacgtct
    34921 ataatagaag aaaagagtgg tttgcccact ctttttactc tctttatcca tagaaattgg
    34981 actcagccaa gacttgtgat ataatataga aagcaaaaag gcagacgcct agataatagg
    35041 agaaactatg tcaaaagata tccgcgtacg ttacgcacca agtccaacag gactactaca
    35101 catcggaaat gcccgtacag cattgtttaa ctacttgtat gcgcgccatc atggtggaac
    35161 atttctcatc cgtatcgaag atactgaccg taaacgccat gtcgaggatg gtgaacgttc
    35221 acaacttgaa aaccttcgct ggttaggcat ggattgggat gaaagtccag aatcacatga
    35281 gaattatcgc cagtctgagc gtttggactt gtatcaaaaa tacattgacc aactattagc
    35341 tgaaggaaaa gcctataaat cttacgttac agaagaagag ttggcagctg aacgtgaacg
    35401 tcaagaagta gctggcgaaa caccacgcta catcaatgaa taccttggta tgagtgaaga
    35461 agaaaaagcg gcttatatcg cagaacgtga agcagctggt atcataccaa cagttcgttt
    35521 ggctgtcaat gagtcaggta tctacaagtg gcatgatatg gtcaaaggtg atatcgaatt
    35581 tgaaggtggc aatatcggcg gtgactgggt tatccaaaag aaagacggtt acccaactta
    35641 caactttgcc gttgttatcg atgaccacga tatgcaaatc tctcatgtta tccgtggaga
    35701 tgaccatatt gctaatacac caaaacagct tatggtctat gaagctcttg gttgggaagc
    35761 tccagagttt ggtcacatga ccttgattat caactctgag actggtaaga agttatctaa
    35821 acgtgatact aatacacttc aatttatcga agattaccga aagaaaggtt accttccaga
    35881 agcagtcttt aactttattg ctcttcttgg ttggaaccca ggtggtgaag atgagatttt
    35941 ctctcgtgaa gaactcatta aactttttga tgaaaaccgc ctcagcaagt caccagcagc
    36001 ctttgatcag aaaaaattag actggatgag caatgattat atcaagaatg cagaccttga
    36061 aaccatcttt gaaatggcaa aaccattctt agaggaagca ggccgtttga ctgacaaggc
    36121 tgaaaaatta gttgagctct ataaaccaca aatgaaatca gtagatgaga ttatcccatt
    36181 gacagatctt ttcttctcag atttcccaga attgacagaa gcagagcgcg aagtcatgac
    36241 gggtgaaaca gttccaacag ttcttgaagc attcaaagca aaacttgaag cgatgacaga
    36301 tgatgaattt gtgacagaaa atatcttccc acaaattaaa gcagtccaaa aagaaacagg
    36361 tattaaaggg aaaaatcttt tcatgcctat tcgtatcgca gtttcaggcg aaatgcatgg
    36421 gccagaatta ccagatacaa ttttcttgct tggacgtgaa aaatcaattc agcatatcga
    36481 aaacattcta aaagaaatct ctaaataaag aggatacaat aatggacatc tgggaaaaga
    36541 tgtacgaaga agcacagaaa ctatacaatc cacatgaagt atcagacttt gtttatgcca
    36601 atcatgttgt agctgcagta gaagcaggag atggacaaat atttacagga ttctgtatgg
    36661 agggaacctg tggtgttttc catctctgcg cagaacgggc gaccctcttc aatatgtacc
    36721 aattttcagg acaaactaag gttaagaaag tattagcctt tcgagacaaa ccaccttatg
    36781 gtggaagttc agccatgcct tgcggtgctt gtagagaatt ccttttagag cttaatgctg
    36841 aaaataaaga tgcagaattc atgatggact ataatataag aaaaacagtt aaagtcgcag
    36901 aactaatccc ttattggtgg ggagaagaac gtgcttctaa gtttaatgag tgatagaaga
    36961 gtcagtataa ttctggctct ttttactata agttgaaaaa ataaaaaaaa tta
//
LOCUS       DAVKGU010000011        35623 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKGU010000011 DAVKGU010000000
VERSION     DAVKGU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.011"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..1344)
                     /locus_tag="VW077_000514"
     CDS             complement(<1..1344)
                     /locus_tag="VW077_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HEU9454588.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKSEAAKKHYEEVKKKAEDAQKKYDEGQKKTVEKAKREKEASEKIAEATKEV
                     QQAYLAYQQASNESQRKEADKKIKEATQRKDEAEAAFATIRTTIVVPEPSELAETKKK
                     AEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQYEISTLEQEVATAQHQVD
                     NLKKLLAGADPDDGTEVIEAKLNKGEAELNAKQAELAKKQTELEKLLDSLDPEGKTQD
                     ELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAALQNKLATKKAE
                     LEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPTPAPKPEQPAPAPKP
                     EQPTPAPKTGWKQENGMWYFYNTGGSMATGWLQYNGSWYYLNANGAMATGWAKVNG"
     gene            complement(1630..1908)
                     /locus_tag="VW077_000515"
     CDS             complement(1630..1908)
                     /locus_tag="VW077_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454589.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            complement(1893..2132)
                     /locus_tag="VW077_000516"
     CDS             complement(1893..2132)
                     /locus_tag="VW077_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454590.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            complement(3265..3993)
                     /locus_tag="VW077_000517"
     CDS             complement(3265..3993)
                     /locus_tag="VW077_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454591.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKAKEYELFKNSK"
     gene            complement(3990..4343)
                     /locus_tag="VW077_000518"
     CDS             complement(3990..4343)
                     /locus_tag="VW077_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9454592.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            complement(4677..4982)
                     /locus_tag="VW077_000519"
     CDS             complement(4677..4982)
                     /locus_tag="VW077_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPAR17_0115 family bacteriocin-like peptide"
                     /protein_id="HEU9454593.1"
                     /translation="MELILPNNYVDLEQEEMMYLDGGEPMSISAIVALAITIGGASYS
                     TGYVLGERVYYSGYKNSDYQKNKWGIRGAMLAAFGAIGAVVMLGFENRYYALVTGRG"
     gene            complement(5130..6482)
                     /locus_tag="VW077_000520"
     CDS             complement(5130..6482)
                     /locus_tag="VW077_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU9454594.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLEKLRAKEAIFQKFEQSLNEKYNHLSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDADLQALQNELLQLQQQGDSPSLSDTTSADDKAK
                     LETKISEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNNPTSQAYNIYAQLISEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLVPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKADVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(6479..6946)
                     /locus_tag="VW077_000521"
     CDS             complement(6479..6946)
                     /locus_tag="VW077_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9454595.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(6939..9158)
                     /locus_tag="VW077_000522"
     CDS             complement(6939..9158)
                     /locus_tag="VW077_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU9454596.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDIILSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIIDKTTIVVAHRLSTILR
                     CDKICFLENGTIVEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDIEEVTYG"
     gene            9624..10487
                     /locus_tag="VW077_000523"
     CDS             9624..10487
                     /locus_tag="VW077_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9454597.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILSHLKK
                     EVEIYYQAKER"
     gene            complement(10776..11564)
                     /gene="argH"
                     /locus_tag="VW077_000524"
                     /pseudo
     CDS             complement(10776..11564)
                     /gene="argH"
                     /locus_tag="VW077_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            complement(11624..12820)
                     /locus_tag="VW077_000525"
     CDS             complement(11624..12820)
                     /locus_tag="VW077_000525"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU9454598.1"
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDF
                     IHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKYPLVSALSRPLISKKLVE
                     IAHQTGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKEN
                     GVPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEI
                     EFSEGVPVSLNGEVLKLADLIQKLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAV
                     TLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQALIAYIKETQKVVN
                     GTAKVKLYKGSAQVVARKSPNSLYDENLATYTSADTVDQDAAVGFIKLWGLPTKVHSE
                     VQKSAK"
     gene            complement(12837..13643)
                     /locus_tag="VW077_000526"
     CDS             complement(12837..13643)
                     /locus_tag="VW077_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9454599.1"
                     /translation="MNKMKKVLMTMFGLVMLPQLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(13850..14491)
                     /locus_tag="VW077_000527"
     CDS             complement(13850..14491)
                     /locus_tag="VW077_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454600.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(14488..16596)
                     /locus_tag="VW077_000528"
     CDS             complement(14488..16596)
                     /locus_tag="VW077_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9454601.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(16648..16935)
                     /locus_tag="VW077_000529"
     CDS             complement(16648..16935)
                     /locus_tag="VW077_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9454602.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            17366..17686
                     /locus_tag="VW077_000530"
     CDS             17366..17686
                     /locus_tag="VW077_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9454603.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            complement(17739..18326)
                     /locus_tag="VW077_000531"
     CDS             complement(17739..18326)
                     /locus_tag="VW077_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9454604.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            18568..19239
                     /gene="sdaAB"
                     /locus_tag="VW077_000532"
     CDS             18568..19239
                     /gene="sdaAB"
                     /locus_tag="VW077_000532"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9454605.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            19248..20120
                     /gene="sdaAA"
                     /locus_tag="VW077_000533"
     CDS             19248..20120
                     /gene="sdaAA"
                     /locus_tag="VW077_000533"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9454606.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            20142..20774
                     /locus_tag="VW077_000534"
     CDS             20142..20774
                     /locus_tag="VW077_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9454607.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            20800..20898
                     /locus_tag="VW077_000535"
                     /pseudo
     CDS             20800..20898
                     /locus_tag="VW077_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(21080..22930)
                     /locus_tag="VW077_000536"
     CDS             complement(21080..22930)
                     /locus_tag="VW077_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9454608.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQEIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(22972..24129)
                     /locus_tag="VW077_000537"
     CDS             complement(22972..24129)
                     /locus_tag="VW077_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9454609.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIIEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            24187..25353
                     /locus_tag="VW077_000538"
     CDS             24187..25353
                     /locus_tag="VW077_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9454610.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            25492..25818
                     /locus_tag="VW077_000539"
     CDS             25492..25818
                     /locus_tag="VW077_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9454611.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            25805..26398
                     /locus_tag="VW077_000540"
     CDS             25805..26398
                     /locus_tag="VW077_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9454612.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            26391..27302
                     /locus_tag="VW077_000541"
     CDS             26391..27302
                     /locus_tag="VW077_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9454613.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDFITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTDNHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            27364..28428
                     /locus_tag="VW077_000542"
     CDS             27364..28428
                     /locus_tag="VW077_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454614.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVSDKAEETIADFDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEVGEARTEIEEKKASNSTEEESDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(28606..29466)
                     /locus_tag="VW077_000543"
     CDS             complement(28606..29466)
                     /locus_tag="VW077_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9454615.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPLSFYHQLDANQIEVQPILD
                     KDFKALAQEE"
     gene            complement(29588..30574)
                     /locus_tag="VW077_000544"
     CDS             complement(29588..30574)
                     /locus_tag="VW077_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9454616.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(30846..32321)
                     /locus_tag="VW077_000545"
     CDS             complement(30846..32321)
                     /locus_tag="VW077_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9454617.1"
                     /translation="MKNWKKYAFTSASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(32579..33502)
                     /locus_tag="VW077_000546"
     CDS             complement(32579..33502)
                     /locus_tag="VW077_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9454618.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(33516..34445)
                     /locus_tag="VW077_000547"
     CDS             complement(33516..34445)
                     /locus_tag="VW077_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9454619.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(34764..34985)
                     /locus_tag="VW077_000548"
     CDS             complement(34764..34985)
                     /locus_tag="VW077_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454620.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(34939..35199)
                     /locus_tag="VW077_000549"
     CDS             complement(34939..35199)
                     /locus_tag="VW077_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454621.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT        10876 a         7534 c         6018 g        11195 t
ORIGIN      
        1 accgttgact ttagcccaac ctgttgccat agcgccgtta gcgtttaggt aataccatga
       61 accattgtat tggagccaac ctgtagccat tgaaccacca gtattgtaga agtaccacat
      121 accgttttct tgtttccagc ctgtttttgg tgcaggagtt ggttgctctg gttttggtgc
      181 aggagctggt tgctctggtt ttggtgcagg agttggttgc tctggttttg gtgcaggagc
      241 tggttgctct ggttgaggag ccggcgctgg agtttcttct tcatctccat cagggcctaa
      301 ctcattaaga gctgcatcta attctttttg agttttttcc aattcagctt ttttagtagc
      361 taatttattt tgaagagcag cagtatcatc ttcagaatca gcccctccaa gtaatatttc
      421 aaggttacta atttcttttt ctaaatcagc aactttattt tgaagttcat cagctttttt
      481 atccaactca gcttcttctg cttctttatc taattcatcc tgagtcttac cttcaggatc
      541 aaggctgtca agaagttttt caagttctgt ttgttttttt gctaactcag cttgtttagc
      601 gtttagctca gcttctcctt tgtttaattt agcttctata acttctgtgc catcatcagg
      661 atccgcacca gcaagaagtt ttttcaaatt atctacttga tgttgagcag tagcaacttc
      721 ttgttccaaa gtagaaattt catattgaag tttttcaatt tcaagttcct tagcctctac
      781 ttctttcttc gctagtgcta cctttagagt tgcataatca tattttctct tagctacttt
      841 ttcttctgct ttagcttctt ctgctttttt cttagtctca gctaactcac ttggttcagg
      901 aactacaatt gttgttcgaa tagtagcaaa tgcagcttcc gcctcatctt tgcgttgcgt
      961 agcttctttt atcttcttat ctgcctcttt tctctgactt tcgttgctag cttgttgata
     1021 agctaggtac gcttgttgaa cttcttttgt tgcctcagct atcttttcag aagcttcttt
     1081 ttctcgcttt gctttttcta ccgttttctt ctgaccctcg tcatatttct tctgagcgtc
     1141 ttctgctttc tttttaactt cttcataatg tttcttagca gcttcagatt ttttcactgc
     1201 tgcatcatag tctttctcag ctttagactg actagctacg ggagcttctt ctgctcttac
     1261 aaaagtaggc tgagacgtaa caaaaccagc ccctaagata gcgacgctgg ctagacttgt
     1321 taaaatcatt tttttcttat tcatctaaat ttacctcttt tctgatagtc atttttagac
     1381 tatacttata ttaagtatac tccccccgta aatatttgtc aaatgtttta tattacattt
     1441 ttattacaaa tttctatatc ataagcttct actccaaaaa gataaagaca ctacttttgc
     1501 ccttttcgat tctcttttta aacatagcat catcttcctt cattcctata aaaacatttc
     1561 caatgagaga gcttattttt taatttcgtt aaacaacaaa aaacgcaagc ttaagcctgc
     1621 ggacaatctt tatagccatt tcatttcttt cacatctgtt ttacgaactt tagcatattt
     1681 cttagcgctt gtaaaatgta gagtaactga tttaaatact ccgcttttgt ctcttcgtaa
     1741 acctaaaact gcggatcttt tatttttatc ctcaaaaaat atgacgtcca atttcatagt
     1801 atttttagat aaatccctag ctacgatgca acaacttatt attttagaat agaaaatatc
     1861 accgataaac tcatataaag atatcctttc gattacatca ttgtacatag gatatttcta
     1921 aaatcttcta aaataaaaac accagcttga atagcaggtt ttgtttgact agcataatct
     1981 cgtgtgattt tgtataagtc aaataaatga tgtaaatcag tcaagcaaaa attcactttg
     2041 aaaatctcta aatacttata attcgttgat acttgacatg tttttccatt gaatctttct
     2101 tgatagtcca aaatacaatc aagtaatttc aacgacaaac tccatatttt ctcaaaataa
     2161 aaaagcacgg ggcagttctc actactgccc aaagtggttg attagagtcg gcttctctaa
     2221 ctccctatgt tgtgcttatt aggcttatcg caagggaaca gtttttagct ctgccaagcc
     2281 acatagtgtg cttactacta tgtgtccatg tcaccatgaa ttgccgaacg gtttaacgag
     2341 aaagtatagt agattgaaac aagatgtgaa caaatcgatt aggaaagtca aattaatttc
     2401 tagaaatatt ttaacagtca taacggacta ttccagattc aatctactat attactttta
     2461 ggtttatccc aactcaatta tgacattttt ttcaaaagtc aatatatctc actcttttca
     2521 acgacaagaa agaggctgat aatcgaccaa cctctgtttt tagatacagt tcaagtccaa
     2581 actattcttt atagaaaagt ccgataaatt cgagcaatag ttaacatact tacaaatata
     2641 actcccaaaa tagataacac tattttcaca aaactcagtt ccgaacctat gacatatata
     2701 tgaaaaagag gggctagtcc tacacatata tacaataagt agccaacaag ttttatctca
     2761 ccaattcctg caaaaaaatg tttcatgtga cgattctcca tttactccat taaatcagtt
     2821 ttattttaga ctatcataaa acaatcacct cttcaataaa aaccatttgc gaaatactat
     2881 tctgaagcat tgatagctca gatttctcag aatagagctg tcctaactat cgagcagctc
     2941 aaatacatat atatgagcaa ttctaagcct agaattgcta taaaataaat gtttccaggc
     3001 tattctaagt ctttgactgc ttaaaatttt acattctgaa ggattctaaa gctagaacag
     3061 ctcaaaatca cgagaactaa ggtattcgaa acttcagagt aatacaatcc ccataggaat
     3121 tctccattct tattaagcat gattaaaatg taatcctaac aagtgatcct cgactgtgtt
     3181 tgtagcactt agttacctag ataagactat ttccctttat catatctgat tttaagccgt
     3241 tctaagattt aacctatgtt tctattactt gctattttta aataattcgt attcctttgc
     3301 tttgataaat ggaactgaaa aattagaaaa aagaagtaaa accgaaaaca aatagcctgc
     3361 tttttgaatc actaaaagat atcctaaaaa taagtaagct ataacatcca atgcgaaaag
     3421 aaaaaatatt ctcctcttca ttaaagtact tgtgtcaaat aatctgatga ataaaaattt
     3481 tatcgtagta tctacacaaa tttttatcaa aaagataaga gccaatacac gattatcaac
     3541 tgtaccgtaa agaatagatg gaaatatagt taatataaaa ctagaaatct ctaaatattt
     3601 tatccaagga gattgagaca ccttgcttct aaactgacta attttcttat attcgttagt
     3661 caatttagta gcaaaatctt ccccaattaa atgaaagatt gctaactcta ggataaaaac
     3721 acttattaat acggtataag tcaaaaaact ttctgggaaa aacttattga ttaaaatggg
     3781 gaaactcatt aacagaatcg aatttatggc tagacagata gatactattt tatttttcac
     3841 agaatataga ggtagtgata ctattgagta gataaaagct aaaaaaataa gcaaaaaata
     3901 ctccgtgtca tttataatcg ccctgaatga taaaaatata tacatccaac atacgataat
     3961 gtgtattttt cgaatcttat ctacattact catcttactg tcgcagatat gaatccatcc
     4021 caaggataag ggttcccgct aatatcacaa cctctattaa gagcaccata tccaatacag
     4081 taagctattc ttcctgccgc cccagccaat acacctgctg ctgcaccaat aagccaacct
     4141 ccaaggcctc caactttaat atagtttatt agttttttgg ttaaaactgc agctccagct
     4201 aatgctataa aagttcctgg actcatagct aaagctgcgc aaattccttg acaagcacgc
     4261 ttgcttaaat aagctccccc atcaagatac atcatctctt cttgctcaag agcaacataa
     4321 ttatttggta atacgagttc catttatttt ctcctttttt attaagcatg gttaggatag
     4381 tttcctaaca agtgatcgca ggctttgttt agttacctaa attagacttc cttcctttat
     4441 cttattttaa atatttaaaa ccagttactg cttgaagctt aacagtttac ttaattcaaa
     4501 taacagctaa tcactgattc cctaatttta tcaaatatac taataacaac aattgtgata
     4561 cgatcatcat tataagaaca acatttaacg aatcaaagtt tttccgagat aataataaag
     4621 caacagcaag cagtattatt actaaagatt gaataactac tattactctt aacattttat
     4681 cctctaccag taaccaaagc atagtatcta ttttcaaaac cgagcataac tactgcacct
     4741 attgctccaa atgcagccag cattgctccc ctaatacccc acttattttt ttgataatca
     4801 gagtttttat atcctgaata gtagactctt tctcctaaca catatcctgt gctatacgat
     4861 gcgccaccaa ttgtgatagc caaggcaaca atggccgaaa ttgacattgg ttctccgcca
     4921 tcaagataca tcatctcttc ttgttcaaga tcaacataat tatttggcaa tatgagttcc
     4981 atttattttc tcctttttta ttaagcatgg ttaggatagt ttcctaacaa gtaatcgtcg
     5041 actgtgtttc tatcacttag ctacctagat aagatatcct ttctctacct tatttatttt
     5101 tataggttac ctaaggggtt taaaaccaat tattgcttga aacttaacat ttctaaaatc
     5161 caatcaagat aggtttcttt atcatagaca atccgcactt caactggcat atccttttgg
     5221 aggacgactg tctcacttcc atgttttaga gttaaggttt ctaattctat catgacttta
     5281 tagaggctaa tattcccctc tttcgtttct tgggaaattg ttcctgaatc aatctgtctg
     5341 acttgtccct ttagtgttcc atatttttga ctatttacac cagtaatagc aacatcagct
     5401 tttgctcctt tcgaaacacg agaaatatca ctcgcaagta caaaagcttc tacatagtaa
     5461 cctttttctt tccctgaaac ttctgctatc gtttgcccct gctgaataga cattccttgt
     5521 ttcaaaggta ccagataatg cagagtacct tcatttggcg ctaatatact atgtgcttta
     5581 tcttgacctg ttgccactcc aagattagcc tcaagctctg taatactttt attgttgttt
     5641 gaacgagcag tacctaactc actaattaat tgagcataaa tattataagc ctgagaagtt
     5701 ggattattat aggtacggtt catatcctta atattgcttt gttgactatc aatctcgcta
     5761 ttcttagaag taatatttgt ttttaatgct tcgatttttg ttgttatttc tgatatctta
     5821 gtttctaact tagctttatc atcagctgat gtcgtatctg ataaagaagg tgagtctcct
     5881 tgctgttgaa gttgaagcaa ttcattttgt agagcttgca agtcggcatc taactgattt
     5941 ctttcagctg ttattttatt caacttcgta tattcatcct gaatctttga atactgggta
     6001 ccattattat aattttccga gtttaactga gacagatagt attcaacttt cccataatat
     6061 tcctgttctt caccagaatt agataaatga ttgtatttct catttaatga ttgttcaaat
     6121 ttttgaaaga tagcttcttt tgcacgtaac ttctccaatt gtttatttaa agagctcgct
     6181 tgtaaacctt cttgtccact tgataacgaa ataatgacat ctccagcatt taccatatct
     6241 ccttctgact tatgtaaagt aactaccttc cctgaaccaa ttgctgatag gaactctgta
     6301 cctgttataa ctgaatttcc attcgctttt acaatatagt ttttgggtat ataagctgcg
     6361 ccaaccaatg caccgcttaa gataatagca gttgaaataa tgagaataaa cgcaaaagct
     6421 ggtggtctct tatcaaagaa aatacgagaa taacgtaatt ctgatttatt atataatttc
     6481 ataggcttac aattggtcta aaaatatcta ctaccatttt ttcaggagaa gaattaacat
     6541 aaactgtata gacaatccca tccgtttgaa tatcattttc atagacatat agatccaatt
     6601 tagaatacgc atactgtaga tactctggac tgtcttcaaa acgaacatat aaacaatatg
     6661 gaacagagat agaatcctgt acatcataaa tgttactgta ctgttgagca ttatgagctt
     6721 gaatataaaa ctcaaaatca gtcgttatta atccatcatc atgaatagta gtaccacaac
     6781 tttttacaat taatggacca aaaatttgtg cttttaacaa ctgcaaatgt tgatgaaatt
     6841 tattaatttc ctgatctaca tcttctactt ttgtatcatg taacttttta cagataactg
     6901 actttagtac cagtttttta ttatctttta cctctaactt agccataagt aacctcctct
     6961 atatctaaca cagcctgtga ctgaatttgt tgattcactt gaacgctctg caaaccaacc
     7021 attctagcat actttccatt tttcgccatt agttcttcat gagtcccata ttccacaata
     7081 gtcccatttt ctaaaaagca tatcttatca catcgaagaa ttgtagacag cctgtgggct
     7141 actacaattg ttgtcttatc tattatttta ttaaagatta aatcctgaat aatctgttca
     7201 ctaaatgaat ctaagttaga ggttgcctca tcaaatatat acaaatcagc tttactcagt
     7261 agtgctcttg caatagccaa tcgttgtttc tctccacccg agaaattttg accgccttct
     7321 tctacaaagg agccataacg attttgaaga cgttgaatcg tatcatgaat accaacaata
     7381 cgacaaactc tcaccatatc ctcatatgta acagaaggat taccaatttt tagattatca
     7441 ataattgtcc ctgtaaagag ttctatggtc tgaggaatgt aggaaatttt cttacgtagt
     7501 ttaccatagt caagatctga taagtcattt tctcctatac gaattttccc ctcactcgga
     7561 tttataaatc ttaataacaa acgaactagc gtactttttc ctgctccgct ttctccaaca
     7621 aaagctattt tttctccttg tttaattcgc aaattaaagt ccttgataac aggagggcgt
     7681 gaaccatagg caaatgttac atctttaaat tcaatgtcat cacataaggt gaagtctcta
     7741 atactataat ccaaggcaat gtcttcacga tctacttcca tcaattcttg aagccgacta
     7801 accgcaacct gtacctcttg aaaggttaac tgcaagccaa ctaaattctg aataggttct
     7861 gtaaaatatt gacttaatgt ctgaaaaacc aagaggtccc caatagtcat ttttccatca
     7921 atgataaata aggcgcccac tcccataaat aataacccac ccatggttga ggtcattgaa
     7981 gatattgtag attgaatatt ctgcaaaacg ccctctttat aaccaatttc taaagtatga
     8041 acaaattttt cttcaatttt gtttaaacgt tgttcctcat catgctgaga cttaatggta
     8101 tcaatgttac gaatagattc aattaactgt gaattaagaa gtccatttgc ttccatttgt
     8161 tcatgattaa tttttttata aggcttttta aaacagtata taagaatgat gttaacaata
     8221 accataaaaa ctaatatcag aaataaagat tgattaatag tccaaaggac aaccgcacta
     8281 ataactgaca agatgatatc catgactaat gaaatagaga ctgacgtaaa cacattcttt
     8341 atcgtcatcg catcttgaaa tcttgttaaa acatcaccaa ctctacgact tccaaaaaaa
     8401 ctataaggaa gatgaataat atggtcataa tatcccatta aaaccggtat atcaattttt
     8461 cgagatagaa acaaaagaac gtgctgacga aatgcagata gaagagtctg taagaacgac
     8521 actattccaa atactatcaa aaacatatac agactatttt ttaaagcata tggaatcacc
     8581 tcgtccatga taacttttga aaagagactc gataaaatac caatgatact taaaacaaag
     8641 gaagctaata tgactgtact aatcaatcct ttttgattaa aaattaacct tccaaataaa
     8701 tcaagaagtc cttttccctt tagtttccct ctttcaaaat ccgaatttgg aaccataaaa
     8761 agagtaatac cttgataaat ttctcccagt tcatcacttg acatcttgcg tatgccacta
     8821 gcagggtctg ctacgtaaaa tttactattc cttttgatac tatgtaatac cacaaaatga
     8881 ttttgaccta aatcattctt aacttgaaga atcgcaggaa atgttaattt tggagtcaaa
     8941 tcttccagtg ctactcgaac agcttttact gtaaaattca acttattcag acctgaaaca
     9001 ataccactga cagtcgttcc atacatgtct gtaccaataa tttccctaat cttcatgatt
     9061 gatagttcct ttttgtaaga taaaagaact gttgaaacaa ctgccgcagc acaatctgac
     9121 tggtcatgtt gcatcgtaca aggatatttt tttaacatag aaatactcaa ttctttagaa
     9181 ttcatctaac ttttctttga tgtcacccaa acctcaatca ctgcccagta gttcttgaat
     9241 taatttattt tcaaccgtat cattctttgt tcttgatatc tttccaagca ttgtagtaca
     9301 gcagattttt gcccaaggac tgagcctcct ttaacaactc aaactccctt acattatatc
     9361 ggccagcccc atggaaagat tatagccaga ctaatcaatg atataataca tactagaata
     9421 caaaatattt ttttattcat actttttctc ctatctttat tttctaccta catagtacct
     9481 cttattaagc tcattttaaa attacgttaa cttttttcac aaatttattt cgactttctt
     9541 ttttactttt tacattgcta tgatacaata tttttaacaa aattttattg tttgttaact
     9601 tttttcacga aaaggagaaa atcatgcgtt atgatttcgg aaaggtctat aaagaaatac
     9661 gtgagtcaaa ggggctgacc caagaagagg tctgtggggg tgttctctca agaaccagct
     9721 tatcaaaaat cgaaagtggc aagacaaccc caaaatatga aaatatggaa tttcttctcc
     9781 gtcaaatcaa tatgagtttt gaagagtttg aatatatctg tcaactttat caaccaagtc
     9841 aacgcacaga gattatgcaa acctatctca atatgcgttc cattatcggg accagcgatc
     9901 ttgtcaattt atttcaaaaa tgccaagact acctcaagac ccaccacgat ctccctatag
     9961 aagaaatcag agatatgctt gaagttgtta tttatctccg tcaacacggg gcaggggaac
    10021 tatcaaaaca cgcagaacag gttgtcaaaa aactttggaa aaaaattgaa aaacaagata
    10081 catggtatga gagtgatcta aaaatcctca ataccattct tttcagtttt cccattgaat
    10141 acctccatct catcactgga aaaatattgc aacgcctaga agtctataaa aactatcaac
    10201 atttatatga cttgcgaatg acaattctat taaacctttc cacactctat ctatacaatc
    10261 aagataaaaa catgtgtaag caaatctgct acactttact ggaggacgcc aagaataaga
    10321 aaagctacga taggctggct atctgctatg tccgtattgg gatttgtagg gatgatgcga
    10381 aacttatcca aaaagggttc tcccttctgg agctaaccga ggaaacttct atactgtctc
    10441 atctcaaaaa agaagtagag atctattacc aagcgaagga aagataaaaa agccgaggga
    10501 tttcctcgac tctttacata ttatttgttc gttagttctt aataccagcc attgttaagc
    10561 cagaagtttt tagctctgcc aagccacata gtgtgtttac tactatgtgt ccatgtcacc
    10621 atgaatcgcc gaacgattta acgagaaagt atgactttta cgtttatccc aactcaatta
    10681 tgacattttt ttcaaaagtc aatatatctc actttttcaa cgacaagaaa gaggctgcca
    10741 atcgaccaac ctcttattct gaacccatca ctccatcact ttttagcttc attcgctttc
    10801 ttagcgactg caatctggta ttcgacttgg tcaaaccctg tgcctcccaa ggaatttcgt
    10861 ctttgaacag cagttttagg ttgcaagtag acatagatat cctcggtaat gagagaatga
    10921 taggtttgta attcctccaa agtccaatct tgaagatttt tagcagactt gatagagtct
    10981 agcactaatc ttcccacaac ctcatgagct tctctaaatg gcaagccttt ccctgccaaa
    11041 taatctgcca actcagttgc atttgaaaag tccttctctg ttgattcttg cattttttcc
    11101 ttgtttacct gcaaactaga taacatacct gccaacacat cgagggaatt taaaatcgtt
    11161 tcgaccgtat caaacattcc ttccttatct tcttgcaaat ccttattata agctaaaggc
    11221 aaggacttca tgactgtcaa cagtccaaac aaatgcccat aaactctgcc agtctttcct
    11281 cgaatcaact cagccatatc tggatttttc ttttggggca taatagagga acctgttgta
    11341 aacgtatctg acaaggtaat gaattggtac tcaaaactac accaattgat catttcttcg
    11401 caaaaacgac tcatatgcat catgagtatg ctggcatttg atagaaattc taagataaaa
    11461 tcgcggtcac taacagcatc caaagaattt gtataaggtt gcttaaactc cagcaaatcg
    11521 ctagacaatt ggcgatcaat ggggaaagtc gttcctgcca aagcgaccac cccataattt
    11581 tgtattctta gccatatcac atccacctct ctatccaatc gtcctatttg gcgctttttt
    11641 gaacctcaga atgaaccttg gttggaagtc cccataactt aataaagcca acagccgcat
    11701 cttggtcaac ggtatctgca ctagtatacg tcgccaaatt ttcatcgtaa agagaatttg
    11761 gtgatttccg agccacgacc tgagcgctcc ccttatacag tttgactttt gcagtcccat
    11821 tgacaacttt ttgagtctct ttaatataag caatcaaggc ttgtgtagcc gaactaaacc
    11881 acaaagcatt ataaatgaga tttgataatt cattttctat gattggttta aaatgagcca
    11941 cttctctcac aagcgtcaag tcctcaattt ccttatgagc tgtcagcagg gtcactgcac
    12001 ctggacactc atagatttct cttgatttaa tcccaaccaa gcgattttcc acgtggtcaa
    12061 tccgtccaac cccatgtttt ccagctattt cattgagttt ttgaatcaaa tctgccaatt
    12121 ttaacacttc tccattgagg gaaactggaa caccttcact aaactcaatc tcaatgtatt
    12181 ctggcatatc tggagcttgc tctggagaag ttgtgatgcc aaaggcttct tctggagcct
    12241 gattccatgg attttccaaa ataccacact cattggcacg tccccaaaga ttttggtcga
    12301 cagagtaggg attgtcaagg tcagcaggaa caggcacccc attttccttg gcataataaa
    12361 tttcttcctc ccgagaccat ttccattcac gaactggagc aatcactttt aaattgagat
    12421 ccaaggctgc aatcgatact tcaaaacgta cttggtcatt ccccttacct gtacaaccat
    12481 gagcaattgt agtcgctcct gtctgatgcg ctatttcaac caatttttta gaaataagag
    12541 ggcggctcaa ggcagatacc aaggggtact tttgttcata gtaggcatgt gactggagag
    12601 ctactagcac ataatctgta gcaaattcgt ccttaacatc aatgacataa gattcgactg
    12661 ccccaacctt aagagcctta tcatggatga aatctaagtc tttcccttca cccacatcca
    12721 tacaaactgc aacaacatca tagtctttct tcagccaggt aatagcaact gatgtgtcta
    12781 aaccgccaga ataggccaaa attacttttt ctttactcat gttcttttcc ttctttctat
    12841 ttttcaatgg atgctttaaa ggcatcctca atgagtttgt ctaattcccc agactccttc
    12901 aacttttgaa tggttttatc gactgcctct ttcaattcct tgctatcttt tttcatggct
    12961 accgcatagg aatcatcttg ctctttttca aaattgaggt ctgcgattgc taaatcagga
    13021 ttattttcca caaatccctt ggcaacgggt tcttcaaaaa taacggcatc cacttgccct
    13081 gattttaaat ctgtgattaa attcccattt ttaggcagag atacgaggga agaattttgt
    13141 agcaaatctt tcgccatcgt ctcttgaatc gaacctttct gcgctccgac ctttttctgc
    13201 gccaaatcgt tgacagactg ataagtggct aaatcagatt ttttgacaat gagtttattt
    13261 tttgcagtat agtagggagt tgaaaaatca aacaccttgc tccgttcatc tgtcttagaa
    13321 acacctgata tggcaaggtc agcttttcct gattgaacgc tagccagtac attgtcaaaa
    13381 ctcataggag atagttccaa ttcgacacct agttctgttg cgatagcctt ggctaattcg
    13441 atatctgaac ccacaatctg atttttccca tcaaccactt tttgatattc aaatggagca
    13501 aaatctggat tgagggctac aaccaatttt cctttggact tgatggcttc aattccagcc
    13561 gattgattgt tactacaagc aaatagttgg gggagcatca ctaaaccaaa catcgtcatc
    13621 aacaccttct tcatcttatt cataacaaag cctcctttat gtttattctt ttttattgta
    13681 taaataatac tcctatactc atcctttgtc aagcctaaat tgaaaaaatt ttcaatttga
    13741 aacaaaaaaa acctagaaat actacataaa atgtcattcc taggcgtatt tatgctattt
    13801 ttctctggaa aacatgagta ttcagccagt caaaaggagc tgaatgaact cattttccct
    13861 cgcccaattc aatgacacga tgacattgtt gggctacata agcatcgtgg gtcacaataa
    13921 tgactgtttt cccttctcga ttcatctcta agagaaactt caagaccaaa tctctatttt
    13981 caggatccag cgaacctgta ggttcatcgg ctaaaatcag ctggctgggt tttaagatgg
    14041 ctctagcaac tgcaattcgt tgttgttcgc ccccagacaa ctcggagacc ctttgatgca
    14101 aagtagctga caaacctact ctctctaaaa tctcttccac ctttttgagc ttgtctttct
    14161 taggcaattt cacatatttc agcgccagca tgagattgta ctcgaccgtt tcatcatcaa
    14221 tcagggcaaa attttgaaac agataagaga tatgttcacg gattattgtt tgcgacttag
    14281 cagaattaac cgctagattt gtctgaccaa aaatctcata ccgtccgcta taatcaccat
    14341 ctatcaaacc caataaattt aacaaggtcg acttcccact accactctta ccaacaatag
    14401 ctaccaaatc cccctgatca atcctgagag ataagttatc caaaatcact tttcccccaa
    14461 tggttttggt aatatttttc aactcaatca taagatgccc cctttcaata actctactag
    14521 acttcttttc tccatcctag aagctaagcc tagcacaaat agtatatcca gacatgtaaa
    14581 acctgcaaac agtagaagtg gtaagaacgc atgggcaaag aaaatcaaga ctagaagagg
    14641 gaaactatag cccagcaaga gcagaacgag gagaggacgg tagcgatcga ccagtttcca
    14701 ccccataaac ttcttggtaa tgatatccct gcgcttcaat aagaaagttg ttactagtaa
    14761 gaagtaggaa atcatcatgc taaggagacc aaacaaagca aagagtaggt taaaattccg
    14821 aacagcatct cgataagaat ccactttctc ttgttgaatg gcttgaatag atgaaaattt
    14881 taaataattt ccatctgaca atttctcaac tagctctgta atctcttttt gatgttgaac
    14941 cgtattttca attttaatcg gattatttaa gccagttgtt gacagggagg ctttctcatc
    15001 ccacatcata tcagaatcat tgaccaagct aataattgga ttggagagat tttcctttcg
    15061 cttatcacta tatgggaaaa atgaccaatc tccttcataa taggcaatct cgacatccat
    15121 ctcctctatc gttcgttttt gctgctcttc atacttcatc gaatgaaagg caattaactt
    15181 ccccaagagc tgatttttat cttcttcacc tttcgtactt gctggcatca aaataacttt
    15241 tttaataccg gtatctggta gcttgaatcc cttgctcttt agaaaattgc gattggcata
    15301 gtaaacatcc accgtatctg ttaactgata ttgctgaatc tgttctgatt ggacaaaatt
    15361 ttttacagga agactgctac tctgcacata gcccgcctgc gttttttcta ccaaatcctg
    15421 ataaaatcga tagaaataat ctgtagattt ccctgaccct gctagctctt cttgccacag
    15481 attatcattg agtttgaagg tttctaaggt caggtaatta ccttgactta cccactgttg
    15541 ctgataagca agttctttgt tttcttgttc taaacttctg cccaccccaa tcagtaaggc
    15601 cgtcagtaaa atagttgtcc ctattttcat cacataattg aagataagac caaatttgaa
    15661 agatgaaaaa cctttcagca gagagctgat tgtcattttt tggattaaga ggtaagtcaa
    15721 ccaactgata aagagataaa gctgcaacag caaaaaatga gacaaccaca gcataggaaa
    15781 caaatctttt ggcttataat caagcaagaa aaacacgcct agattgatca caagagcccc
    15841 acctaggagg aggtaaaggt tgccttttac aacatcagct aaaacagccc tatcttgaaa
    15901 accaagtaat ttttgtaccc caactctttt catctccatc atcggttgat acactgtcac
    15961 taacacaaga agcaaaatag ccaagacaaa aacaatggca gataaaagca aatctcgatt
    16021 tatgacttcc actgcacttt tgtaggtcgg ctctagcaag gtagcctggt ctatcttgaa
    16081 aaaatcgctc catttctgta caatcctatc cttgtccatc tcttgtgtag aagttatcgt
    16141 atagcgacca tttaaactac gagatgtatc cttgatatag gtttgaaaag tcataagctg
    16201 aataggtttg gcttttagaa aggtcggaat cgtaccaagt ttattggaaa tttctttatt
    16261 actatagact ccttcaccat ctgtggtaaa atcaagagaa gaaatcccaa actcttggta
    16321 ggggaaggta tctttatcaa aaacaccaga cttgaccacc tcatcaccac tgtctgtttt
    16381 gatgatggag actttatact cctttgatac atcctcaaaa aatcgaagaa cagacgctgc
    16441 aggttcgtta atatctttca aatacaaatc caaagaatct acagtctttc ccatttcttg
    16501 aatccgaacc acttctcgtg tatagtttac attaaaaaca aaaaaagtgg tacacagaag
    16561 caggagtaac atacagaaat tactgatttt tttcataaaa gtgcctccac tacagttttc
    16621 cctctccaaa taggagaggg aagaatttta ctcaaatcca tagaaatatt ccattcctgt
    16681 tggcggaatc ttggtgaggg aagctctagc ccatgcctca ggttttgcat aatccttaga
    16741 ggttctaccc ccatgtctaa cagttgctgt atgatatcga gtagaatgta agtaatcaga
    16801 atatccaaaa gttcctgtcc aacctactcc atagttccat tgaccaccct caacccatac
    16861 tgctaaagca tttgtagtag caagtatacc acataccatt aaagatgcaa caccaatttt
    16921 aactaatttt ctcatgattt atttctccta ttatcgagag ctgttccgta aaaacagcta
    16981 tagtaattta tcaacaagta tcgtagtcca ttggactcac cttccttcta tctcatttca
    17041 ttttttaaca acgaacttac tcttaaccgg gagaatggtg cgcttctgta tcaggtcctt
    17101 gaacaaccag agtagtatta agcaaactct aactattact caagaatcag tccaataaac
    17161 aatatggata gcgctttcaa atttttaaac tcctatcccg tccttttatc tatataataa
    17221 gcataagcga aaatataata actgtcaagt aactgaagtg aattttataa aaaaattaca
    17281 agccaaattt gtaaagttta cactaagccg ctagggcaat cgtctatcag aatgtccgtt
    17341 tatttgtcaa taatccgaga aaatcttgca acgcttagaa gtctataaaa actatcaaca
    17401 tttatatgac ttgcgaatga caattctatt aaacctttcc acactctatc tatacaatca
    17461 agataaaaac atgtgtaagc aaatctgcta cactttactg gaggacgcca agaataagaa
    17521 aagctacgat aggctggcta tctgctatgt ccgtattggg atttgtaggg atgatgcgaa
    17581 acttatccaa aaagggttct cactcctaga gctgaccgag gaaacttcta tgctgtctca
    17641 tctcaaaaaa gaagtagaga cccattatca accaaagaaa ttataaaaaa agtcgaggga
    17701 tttcctcgac tctttacata ttatttgttc gttagttctt aataccagcc attgttaagc
    17761 cagaagtttt tagcagcagt ccatgaaccg taacgtcctg caacgtaggc atctgctaca
    17821 cgttcttggt tttcagctga gtagtcaccg ttcaggtatg aatctgttaa ttggtaacgt
    17881 ccgatataac gtccatttgt agctgtatag ctaccacctg attctttttg agcgatccat
    17941 tctttggctt ctgcttcaga tccgcttaca gttgaaacaa ctgtttcact tactactgga
    18001 gtttctgcta ctggagctga aacagtttca tcttgagcgg ctggtgccgc ataagtagct
    18061 ggcgctggtg ttgcaacagg cgctacaggg ccatcgataa ccaactcttg atcaacataa
    18121 atcaaatgaa tgttatcaat gtggttgttt tctgccaatt tttcaactgt tgtgttgtga
    18181 gtttcagcga tttctgaaag tgtatcacct tctttaacag tgtaagttga tgattcttga
    18241 gcagatacaa atgatggagc aaatactgca aacaaggcag ctactcctgc aagagttgct
    18301 ttaatctttt tagttattga tttcatattc gaaaattctc cttctttcta tagttctatc
    18361 atacccttta aatattaccg tttcttaaca cttttatgta gaaatattac aaaattattt
    18421 tttatttccc taaataaggt gttttttgtc ataaaagata ctatttttat gctatttgag
    18481 gtattagaaa ggttttcatc cacaattaaa gccaagcccc tcattctttg atataataga
    18541 gataagaatt tttataaggg gaaactgatg aaatcacttc gttttcaatc tgtctttgat
    18601 atcatcggac cagttatgat tggcccatct agtagccata ccgctggtgc tgttcgtatt
    18661 gggaagattg tctcttccat ttttgatgat actccgacag aagttgaatt ccaacttttt
    18721 aactcatttg ccaagaccta tcgtggtcac ggaacagacc tagcccttgt tgcaggtatt
    18781 ttaggaatgg atacagatga tcctgaaatt ccaaatagtc tggaaattgc ccacaagcgt
    18841 ggtatcaaga ttgtctggac cattcagaaa gacagcaatg cccctcaccc taacaccact
    18901 aaaattactg ttaaaaatgc ccacaagacc atcagcgtga ctggtatttc tatcggtgga
    18961 ggaaatattc aggtaaccga actcaatggt tttgccgtct ctctcaatat gaatacaccg
    19021 actatcatca tcgttcatca agatattcca ggtatgattg cccttgttac agaggcgctt
    19081 tcccgctatg gtatcaatat cgcccagatg aatgtcactc gtgaaaaagc tggtgaaaaa
    19141 gccattatga ttatcgaagt tgacagtcgc aactgtgatg aggctatcga agaaattcga
    19201 aaaatccctc atctccacaa tgtcaatttc tttaaatagg aggaagcatg ttttattcta
    19261 tcaaagaatt ggtcgagcaa gcagatctgg actttcaagg aaatgtcgca gaactcatga
    19321 ttacaacaga gtttgaattg accggtcgcg aacgtgaaga agtcttcctt ctcatggaac
    19381 gcaatctgga agttatgaaa gcctctgtcc aacttggcct caatgaaaat aaatctcgta
    19441 gtggcctgac aggtggagat gctgccaaat tggatcacta catcaaaaac gaaaaaactt
    19501 tatcagatta cacgattctc tctgctgccc gaaatgctat cgcagtcaat gaacacaatg
    19561 ctaaaatggg cttggtctgc gctactccta ccgcaggaag tgccggttgt ctcccttccg
    19621 ttctcactgc tgctattgaa aaattagacc tcagccacga gcaacaattg gatttcctct
    19681 ttgctgctgg tgcctttgga ctagtcatcg caaacaatgc ctccatctca ggtgctgagg
    19741 gtggatgtca agctgaagtt ggttcagcct ctgctatgag tgctgccgcc ttgactctgg
    19801 ctgcaggtgg aacaccttat caggccagtc aagctattgc ctttgtcatt aaaaatatgc
    19861 taggcctcat ctgtgaccct gttgcaggtt tggtcgaagt tccctgtgtc aaacgtaatg
    19921 ccatgggagc tagctttgct ttcatcgcag cagacatggc cttggcaggt atcgaatcta
    19981 aaatccctgt ggatgaagtg atcgatgcca tgtaccaagt aggagcaagc atgccaactg
    20041 cctttcgtga aacagctgaa ggtggactcg ctaccacccc tactggtcgt cgcctccaaa
    20101 aagagatttt cggagaataa gcttatcaaa taggagaaac catgacctct atcacagcga
    20161 tttttttcga tttggatgga accctcgttg atagttctat cgggattcac aatgccttta
    20221 cctatacctt taaagagctg ggggtgccta gccctgatgc caaaactatt cgtggtttta
    20281 tgggaccacc tctcgaaagt agttttgcga cctgcctgtc caaagaccaa atttctgaag
    20341 ccatgcagat atatcgttct tactataagg caaaaggcat ctatgaagct caactctttc
    20401 ctcagattat agacttgctt gaggagttat cgagcagtta tccactctac atcaccacga
    20461 caaagaatac atctactgct caagacatgg ccaaaaactt ggaaatccat catttctttg
    20521 atggtattta tggttctagc cctgaagcac cccataaggc agatgtcatt caccaagcct
    20581 tacagacaca tcaactagca ccagaacaag ccatcatcat cggagatacc aagtttgata
    20641 tgctgggagc tcgagaaaca ggtattcaga aattggccgt cacttgggga tttggagagc
    20701 aagcagatct actaaactat caacctgatt atatcgctca caaaccctta gaagttttgg
    20761 cgtattttca atagcataga ctgggcgaca actctatttc agaagaaaat gaagtaatac
    20821 tcaatgaaaa tcaaagagca aactaggaaa ctagctgcgg gctgctcaaa acactgtttt
    20881 aaggttgtag atggaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga
    20941 actgacgaag tcagtaacat atacatacgg caaggcgacg ttgacgtggt ttgaagagat
    21001 tttcgaagag tataagtctc tacataataa aacgcatatt accaaggttg tgtaacttga
    21061 tactatgcgt ttttcttttt tattcaatgt gggttgtttg attagcaaag gcgataatag
    21121 cttgcttcaa ctcatctcca ctgagagtgc ggaactctcc aatcttttga ttgatggatt
    21181 ctaaaggcat gacattaacc ttaccaacga aaatcttatc cataacttga ttatcaacga
    21241 gttcggttga taccaagagt tcttcgtaga gtttttcacc tgggcggatt ccaacttcaa
    21301 cgattggaat ttcactttca gtgtggccac ttagaagcac catcttcttg gccaagtcat
    21361 aaatcttgac tggtttgccc atatcaagga taaagacttc cccatctttg gcataagcac
    21421 cagcatggat aaccagacgg ctagcttctg gaatggtcat aaagtaacgg gtcatgcgga
    21481 agtctgtcac cgttacaggc ccaccttcag caatctgacg ttcaaagact ggaatgacac
    21541 taccacggct accaagaaca ttcccaaaac gaactgcaca gtaggttgat tggctacgtt
    21601 ggttaaagcc agtaacaatc aactccgcca cgcgcttggt tgctcccata acatttggtg
    21661 gattgactgc cttatctgtc gaaatcataa ccatcttaga cactttagct tcatcaacag
    21721 ccttagcaac attgtaagtt ccacggatat tgtttttgaa ggcttctttt ggattgcgct
    21781 ccatcatagg aacatgcttg tgggctgccg catgataaac aatagcaggt ttgtactgct
    21841 caaagacttg caacaaacga tcatagtctt gaatgtccgc aatcacaggt acataatcaa
    21901 tctcttggaa cttacgaatc aattcatgat aaacaaggta gattgagttt tccccatgac
    21961 cgagcaagac aatgcgttca ggattgaagc gactaacttg acgacagatt tcagaaccga
    22021 ttgaacctcc agctcctgtg actaagatgg tcttacctgt cagttctgca cccagacgcg
    22081 attcgtcaag acggatttcc tgacgaccca aaaggtccgt aatatcaatt ttttggaagc
    22141 cagtacctgc ttggtgaagg ccctgaacaa cagtttcaac cttaggcatc ttgtaacatt
    22201 tgacacccag cttattacac atctgcaaga tacgctcata ttctgacgga tccagcgacg
    22261 gaatcgcaac gatgacacgc tcgatttgat ggcgtttggc taattcaggc agattgtcat
    22321 aagagcccaa aacaggaata ccaccaagtt tttgaccctt tttcttagaa tccttatcca
    22381 aaataccgac cagttctaat tcactggttg gatgttggta actatccata aaaagagccc
    22441 caccatcacc ggcaccaatc aagaaggtcc gacggtgttc tccatcacca ctaccttttt
    22501 tgcgtctgga gtagattaac tgccaagtaa tccgtggcaa taaaatcaag aaggtactca
    22561 acaagataaa gagaatgatg aaacggatgg agaagagtgg caagaaggca taacagatac
    22621 tatatgacaa gacactgcta gcagtcacac caaaaaagat tttcatgaaa tccgtaatct
    22681 tgctgtaacg actaatgctc gcgttcaacc cccaaaaacc aatcatcaat tgatagaaca
    22741 ggaaggccaa actcgtatag ataatgtagt caacaggtgc tggattaatc agcccataaa
    22801 ataaaatata agatacaatg atggaaacca ccatactgaa aatatcaaat attccccaga
    22861 aaacctgctt ttgttgttta tttaaaattt ccaccagatc aatcacataa tctgttagtt
    22921 ttttattcat agaaatctac tcctccttaa aagagtttta ctatattgtt attatagcat
    22981 atttttattt atttggctta ttttatccaa ataaattatt gttctcttgt actccttctt
    23041 ccatttaaat cctctaatta aacctacaat cgtcccaaag ccataagcaa agtgaatgga
    23101 aaataaaaga aagggcatca caattagaaa tccattttta tgttttaata aagtcagcaa
    23161 agtgagtagt gacaaaagta gaaaataggc acctaatagt aaagttatga atacgaatgt
    23221 gatcggtaac aatgctagac taaacacaag actcaaaaca aataaacaag gaacatagtg
    23281 aaataatgat aaacacttaa actgaacatg acttgtcaag ccaatccaca aaccatttaa
    23341 atacttttga tgcagcattt tcttgaatgt tggtcgaata tactgataag atagaatact
    23401 tgggctatag cggattttat aaccatattc tcgaattcta taatgaatat cattatcttc
    23461 agttcggcca agttgctcat ttactaaacc aaccttctgg aaaacctctc gtttatacat
    23521 tccatgaaaa atagaagaaa catatctatc ctcagaacta tttcgataat tggcaatgct
    23581 actgccaaac atattttcct caacaagatg caaggtctct gcccattttc cttttccttc
    23641 gataatcgtc ggtctaggcc ccccacagac aaattcacct tgttgaataa tagccacatt
    23701 gttcattaca aaactctcag taacttttga atgagcatca atttttaaaa taaggtcccc
    23761 tacagaatgt ttaactccca ggttaaaacc actagcttga tttttcttag gattgttata
    23821 caatctaatt gagttaaact ctgtatcttc ctttataaat tgctgaatga tagctgtggt
    23881 cccatctgtg gacatagcat ttataaatag aatttcaata ttctctttag gataagtttg
    23941 attttttaag tcttcaatta gaccaggcag atatttttct tcattgtatg cactgattac
    24001 aatggatacg agaagacttt caacattttg agtcactact actctcctga atttttcctt
    24061 ccaccattct atcataattt tcaagacttt cccattttta tcttgaaagc cagaaacctt
    24121 acttgacatt atagtgaaaa agccttaaaa taagctgtta ttaaaatcag ctagaagagg
    24181 tattatatga aaaagcaatc actctttttt gttccaggta ttatcctgat tggtgtttcc
    24241 ttgcgaactc cttttactgt tttacccatt attttgggaa atatttcgca aggtctggag
    24301 gtagaagtta gttcgcttgg tgtcttgacc agcctgcctc tccttatgtt taccctcttt
    24361 tcaccatttt ctacccgact ggctcagaaa atcggcttgg agcatctctt cacctacagc
    24421 ctcttcttct tgaccatcgg ctcactcatt cgactaatca atctgcccct gctctatcta
    24481 ggaaccttga tggttggggc aagtgtcgca gtcatcaatg tcctgcttcc tagtcttatc
    24541 caagccaatc aaccaaagaa aattggtttt ctgaccacct tatatgtaac gtctatgggg
    24601 attgcaacgg ctctggcttc ctatctagct gtgcccatta cacaagccag ttcttggaaa
    24661 ggacttatcc tcctcctcac gttactctgt ctagcaactt ttttggtctg gctcccaaat
    24721 caccgctata atcatagact agctccacaa accaaacaaa aaagtcaaat aaaggtcatg
    24781 cgtaataaac aggtttgggc aattattatc ttttcaggtt ttcaatcctt gatcttttac
    24841 accgtcatga cctggttacc taccatgtct atccatgcag gtctatccag tcacgaagct
    24901 ggcttgctga cttctatctt atctctgatt agcattcctt tttcaatgac catcccaagc
    24961 ctgacaacca gtttatctac tcgcaaccgt cagctcatgc tcactctggt ttcactagct
    25021 ggtgtggtcg gcatttccat gctctttttc ccaatcaata atttcattta ctggcttacc
    25081 atccatctcc tcatcggaac cgcaaccagc gccctcttcc cttatctcat ggtcaacttt
    25141 tcactcaaga caagcgcccc tgaaaagaca gcccaattgt ccggcctatc tcaaacagga
    25201 ggctatatcc tagcagcctt tgggccaacc ctctttggtt acagttttga cctgttccac
    25261 tcttgggtac cagctgtagc tgccctcttg ctcatctata tcctgatgac tgtggccctc
    25321 tttacagtgg acagagcgga taagatcctt taaacttcta gttttagcta gaagtttttt
    25381 atatataaaa attttacaca tttctcttga cagggctttc ttttagatgt acaatgtatg
    25441 tgtagaaaaa ttatatataa aaatcttaca cattagaaaa ggaggtttcc catgtacttt
    25501 ccaacatcct ctgccttgat tgaatttctc atcttggctg tactggagca gggtgattct
    25561 tatggttatg agattagcca aaccattaag ctgatcgcta atatcaaaga atccacactc
    25621 tatcccattc tcaaaaaatt ggaaggcaat agctttctga caacctattc tagagagttc
    25681 caaggtcgca tgcgcaaata ctactccttg acaaacggtg gtatagagca gctcttgacc
    25741 ctaaaagatg aatggacact ctatacagac accatcaatg gcatcataga agggagtatc
    25801 cgccatgaca agaactgaat acctgactca gctagaactc tatctcaaga aactacctga
    25861 agctgaccgt atcgaagcca tggactattt cagagagctc tttgacgatg ctggagtcga
    25921 aggagaagaa gaactcatcg ctagtttggg aactcccaaa gaagcggccc acgaagttct
    25981 atccaatctt ctcgataaaa aaatcaatga agcacccgct caaaaaaata accgacaaat
    26041 tttacatatc gccttgttag ccctccttgc agcacctatc ggcattcctc tgggaatcgc
    26101 catcctcgtg accctgttcg caatccttgt agccgctttg actgtcattc tggctttctt
    26161 tgcagtttcc atactgggta tcatcggcgg attcctattt ttagttgaaa gtttcactat
    26221 cctcgcccaa gccaaatcag cctttatctt gatttttggt tctggtttac tggctatcgg
    26281 tgcttcttcg ctagttttac ttggcatttc ctatgtagct cgcttcttcg gtctactcat
    26341 tgttcgtctg gtacaatttg ttcttaaaaa aggaaagaga ggtaatcagc atgcgtaaat
    26401 ggacaaaagg atttctcatc tttggtgtgg tgactaccgt tatcggcttt atcctgcttt
    26461 ttgtaggtat ccaatctgac gggattaaga gcctactttc catgtccaaa gaacctgtct
    26521 atgatagccg tacggaaaag ctaacctttg gcaaggaagt cgaaaaccta gaaattactc
    26581 tccaccaaca cacgctcacc atcacagact ctttcgatga tcaaatccac atttcttacc
    26641 atccatctct ttctgctcac catgatttta tcaccaatca gaacgataga actctgagtc
    26701 tcactgataa gaaactgtct gaaactccgt ttctctcttc tggaattggt gggattcttc
    26761 atatcgcaag tagctactct agtcgttttg aagaagttat tctccgacta ccaaaaggga
    26821 gaactctaaa agggatcaac atctcagcca atcgcggaca aaccaccatc ataaatgcta
    26881 gccttgaaaa tgcgaccctc aatacaaaca gctatatcct ccgaattgaa ggaagtcgta
    26941 tcaaaaacag taaactcaca acgcccaata tcgttaatat ctttgataca gttcttacag
    27001 atagtcagct agagtcaaca gataatcact tccacgctga aaatatccaa gtccatggta
    27061 aggttgaact gactgccaaa gattatctca gaatcatcct agaccagaaa gaaagccaac
    27121 gaattaactg ggacatctca agtaactacg gttctatctt ccaattcaca agagaaaagc
    27181 ctgaatcaag aggtacggaa ttaagcaacc cttacaaaac tgaaaaaacc gatgtcaagg
    27241 atcaactcat tgcgagatct gatgataata ttgatctaat atccacacca agcagacgtt
    27301 gacaaaaacg cttacatctg ctactatgga agcatattta tctaaccaaa aggaggttct
    27361 accatgaaac aagaatggtt tgaaagtaat gattttgtaa aaacaacaag caagaacaag
    27421 cctgaagagc aagctcaaga ggtttcagac aaggctgaag aaacgatagc cgatttcgat
    27481 acaccaattg gaaaaaatac tcaagtagag gaggaagtct ctcaagctga agtcgaactg
    27541 gaaagccagc aagaagagaa aattgaaact cctgaagtcg gtgaagcgag aacagaaata
    27601 gaagaaaaga aggcatctaa ttctactgaa gaagagtcag acctttctaa agaaacagaa
    27661 aaagtcacta tagctgaaga gagccaagaa gctcttcctc agcaaaaagc aaccacgaaa
    27721 gagccacttc ttatcagtaa atctttagaa agtccttata tccccgacca agctccaaaa
    27781 tctagggata aatggaaaga gcaagtgctt gatttttggt cttggctagt ggaagcgatc
    27841 aaatctccta caagtaagtt ggaaacaagt atcacacaca gttacacagc ctttctcttg
    27901 ctcattctgt tttctgcatc ttcctttttc tttagtatct atcacatcaa acatgcttac
    27961 tatggacata tagcaagcat taacagtcgc ttccctgagc agctagctcc tttaactctt
    28021 ttttctatcg tctctatcct agtagcgaca acactcttct tcttttcatt cctcttgggt
    28081 agtttcgttg tgagacgatt tatccaccag gaaaaggact ggacgctaga caaggttctc
    28141 caacaatata gtcaactctt ggcaattcca atcttcctca ctgctattgc tagtttcttt
    28201 gctttctttg atagcctacg atttacagct ctcttgtgtg tgattagcat tggaatcatt
    28261 ctgcttgcca gtctccatat cattacaaga ccaagtcaag caagtgaaac cgactccttc
    28321 tatcaattat tcttgtctgt ccttgtgaac ggagttatta tcctcctctt ctttgtagct
    28381 gaagtggcac tgattggaga ttatcttcgt atcttggcct ttctttaaac ttcatactct
    28441 tcgaaaatca aattcaaacc acgtcagctt cgccttaccg tactcaagta cagcctgcgg
    28501 ctagcttcct agtttgctct ttgattttca ttgagtagca atcctgtcat ccttggcagg
    28561 attttttcta tgtcaaaaaa gcaagtcgat tgacctgctt ctgctttact cttcttgtgc
    28621 taatgcttta aaatctttgt ctaatatggg ttgcacttct atttgattgg cgtctagttg
    28681 gtggtaaaaa gataggggta atacctctaa gaattttgca tagtccaagc gggcaatggc
    28741 ttcatagtct tctggtttag agccatctcc tgtgatttga accaagtcaa tctcccactg
    28801 cacttcctta tccaccaatt gctcaatata ggctgcagga acaaacggtt ctctcggtaa
    28861 aactgtcttg actccttgag ctagatggta aacgccatgc acttttcctt ctccatcctg
    28921 atagaaggaa actctcgcaa agtaggcatc tgtctcttga accgcacgca cacgcgcttc
    28981 aaactgagcc aagccatctg cctctaaaaa gcttttcaag ttctcacgac taaagaaaat
    29041 aggattaaaa attccttggc aaatcgtaaa acgagctaca gggtctgcta gataggcttg
    29101 aatacttgct tggaaataag cttcaaaatc aaaaccatct agcccttcaa tatcagtccc
    29161 tgtataagca agcaaagcat ctaaaaatga cttgataatc tgccagtctg tctttgttag
    29221 taggtcagga agatcgacac ggtaagcctt ttgaccatca gcataactga ccttaaagag
    29281 aaattgtgat tgccccacta tcgcacactc gatatattcg agacgattaa gaggctgacg
    29341 gagatagact acatcatagc tatgtgactc caaaccgtct agcaaggcca agatcgactt
    29401 ggcattcaaa atctcttgtt ctcctaaaat gctctgttta tttggaataa aaaatgtttt
    29461 tgccataact ggactccttt gaaatcgttt acatacagta taccttattt ttctgaaaga
    29521 tacagaaaca aactttagat ttttgagtac aaagcataga aaaacagccc ctgaggactg
    29581 tttgatctta tacagtagct gcttgatcca agctttcacc gatagcggct aggcgctcaa
    29641 tcacttcagc ttgtgtcaat tcattttttg aaacatagcg gttacgtggg tgaacacggc
    29701 actcgtgtga gcatccacga aggtacttgt cttcattttc ttctgatgtc aagatacgac
    29761 ggttacagaa gggatttcca cagttgacat aacgttcaca tggtgttcca tcaaaccagt
    29821 ctttccctac gatggttggg ttgacatggt tgacatcaac ggcgatacgc tcgtcaaata
    29881 cgtacatttt tccatcccaa agctcacctt gaacttctgg gtctttaccg taagttgcga
    29941 ttcctccgtg caattggccg acatctttgt agccttcacg gaccatccag cctgagaatt
    30001 tctcacagcg aacgccacct gtacagtaaa ccacgacacg cttgtccatg aatttttcct
    30061 tgttatcacg gacccattgt ggtaactcac ggaagttgcg aatatctggg cgaatagccc
    30121 cacggaaatg tcctaggtca tactcataat cattacgtgt gtcaaggaca acggtatctt
    30181 tatcaagaag cgcttctttg aactcttttg gagacaagta agcacctgtt gtttcaagtg
    30241 ggttgatgtc attgtcaaag tcgttgtctt ccaaaccaag gtggacaatt tctttcttgt
    30301 agcgaacaaa catcttcttg aaggcttgtt cactttcttc gtcaatcttg aaccagagtt
    30361 cttccattcc tggaaggctg tgaacgtagt ccatgtattt ttgagttgtt tcatagtcac
    30421 ctgaaactgt tccgttaatt ccctcgtcag cgactaggat acggccttta aggccgattg
    30481 atttacagaa agccaagtgg tctgcagcaa attgctctgc attttcaatt ggagtataaa
    30541 ggtagtaaag taagacacga atatcttttg ccataagatt tgttctcttt tctattctta
    30601 aattttctaa attttttatt tatccatact agtatacctg cttgagaaaa cgaatgcaat
    30661 ttatttgact ggaaattgta gagggttctc gaaatttcct gaatggttaa aataaggaca
    30721 agagaaaaca tggatatcta tatccttgtg ccaaaaaaac cactgccctc cccagaccaa
    30781 cctgaggaaa gcagtgattc ttattttagg agttaggaat gaatacacga aatcaattta
    30841 gctgattatt ttttgttttt caagaattca tcgtattgtt tttgcatttc gttcaatact
    30901 ttttcgtagg caccttcaga tttcaatttt tccatcaatt ctggaatagc tttatctggg
    30961 tctacagtac cagtgttgat agctgtatca aattgttgca ttgtgttagc aatagctgag
    31021 atttcagatt tcacattgtc agtattgaag ataaatccaa gcgctggaga ttctttagct
    31081 tctgccaatt ctttcttaga attttcgatt tgttggtctg taacgttttc gttgatgtaa
    31141 aggatccagt tgttaccagt gttccatcca cccatgtgag tgtttccttt gtagccatca
    31201 agaacgcgaa cacggttttc tttaccttca attttttccc agttcttgcc ttctggaccg
    31261 taaacaagac cgttcaagag ttctggattc gtattcaaga ggttcaagat ttccattgat
    31321 ttttctttgt tcttagagtt gtttgagatg acaaagttag caacttgtgt tgtttggttt
    31381 ttcttgatga agttagtaat tggtttgatt tggatatctt tgttggcaac acgtgaaagc
    31441 aagctgttac cgtagtcagc tggtcctact gtttcttcac gaacgaacca agtatcttgt
    31501 tgaaggtcaa aggaagtatc gcttgttgcg acgtcttttg gaatgtagcc agcttcatag
    31561 aatttgtgaa gagtcttcaa gtgttctttg aaacgaggca cttcgtaacg gtttacaact
    31621 ttagtagtat cgccttcaag gtcgataacg aatggaagac cgtttgctac tgggtagtca
    31681 aagttatcag atgggatgaa aactttacca atagcaaatg gtactacgtc tggagctttt
    31741 tctttaattt gtttcaagac tggctcaaga gtttcgtaag aagtaacacc tgaaatatcg
    31801 ataccatatt tagcaaggag agttccgttg aaggcaaagt tttgagatga tgcaacgttg
    31861 gctgcaactg gaacagcgta aatcttacca tttacagtat tacccttgat gtaagctggg
    31921 tcaagtgctt tgtaaaggtc tttaccttct tttttgtaca attctgtcaa gtcagcgtaa
    31981 gcaccttttt gagcatttac aatatagtta tctgcaaagg caatatcata gttttcacca
    32041 gatgatgtga taactgacat tttcttacca tagtcacccc agccaaggta ttggatatcc
    32101 aatttggcac caactttttc ttcaatgatt ttgttggcat ttgctaacaa ttcatccaag
    32161 ttgtctggtt tgtcaccgat ttggtacatt ttgataacag gtttgtcacc tgaatcagca
    32221 gcttttttgc tgttacctgt caaatttcca caagcagcaa gacctgcagc cagagcgact
    32281 acactagcag atgtaaaagc atattttttc cagtttttca tgataaaaac tccttttttt
    32341 atttttaaac ttataaacaa tgtaatgatc ttatactcaa tgaaaatcaa agagcaaact
    32401 agaaaactag ccgcaggctg ctcaaagcac tgctttgagg ttgtagataa gactgacgaa
    32461 gtcagttaca tatatctacg gcaaggcgac gttgacacgg tttgaatttg attttcgaag
    32521 agtattaact tcacacaagg gaagttggga actgagaaat gttatttctc aataagcact
    32581 attctttcac accaccgata gtcaaacctt ttacaaagta gcgttggaaa aatggataca
    32641 aaatcgcgat tggaagggtt gcaaccacaa ccatggccat acgacctgtt tctttcggta
    32701 gagcaactcc cagttgacca gtcaagccga ccgctttggc aatgtagtcc atattttgtt
    32761 ggatttgcat gagcaaatat tgcaatggat acaagttgtc actcttgatg taaagaaggg
    32821 cgttgaacca gtcattccag aaaccaagag ctgttaagag cgtgatggtt gcgatacctg
    32881 gtagtgacaa tggcaaacag atttggaaga aaatccgggc ttcactggca ccatcgatac
    32941 gagccgattc tagaatggct tctggaatgg tcttcttgaa gaaggaacgc atcaagatga
    33001 tgttaaatgg tgagagaagc attggaacaa tcaaggccca aacagtatcc ccaagctgaa
    33061 gcacacgagt caccatgata taacctggta ccaaaccagc gttgaacaac atactgagca
    33121 gaacgaagat ggtaaagaat ctgcgatact taaaggttgt ccgtgaaata gcgtaggcat
    33181 aggttgttgt gataaagaca tttgtcaatg tcccaactac ggttacaaag acagagatga
    33241 agagggcttg taggatttta tccttaaact gtgccaaaaa ctcaaaaccg tctaagccaa
    33301 attgggatgg gaagaagcta tagccgtatt ggaggaggct tttctcgtct gtcactgaaa
    33361 taatgataac gaatacaaaa ggtaggatac aagagagggc aatcaaaccc gaaatgatac
    33421 tgaagaagat atctgctttc ttactgaagg agtgaatgcc gacattatca attttttctt
    33481 ttttaatttt cttttttgcc atattctcct cctttctaga acaaagctga gtttggatcg
    33541 actcgtcttg caagcaagtt tgataggata accagaatca aaccaacaac ggattggtaa
    33601 agaccggctg ctgcagccat accgatatct gctgtctgag tcaaaccatt aaaaacatat
    33661 acgtccaaaa cgttggttac attgtaaagc tgaccagcat tgtgtgggat ttgatagaag
    33721 agaccgaagt ctgcgcggaa gatatttccg actgcaagga tggtcaatac agttacaagc
    33781 ggagtcaact gaggaatggt tacgttgcga atacgttgcc acttgctagc tccgtccact
    33841 gtcgctgctt cgtagtaggt tggatcaatt cccatgatcg tcgcatagta catgacactg
    33901 ctatatccaa aacctttcca aatacctagg aaaagtagga gatagggcca gatgcccagg
    33961 tcagcgtaga aattgacttc tttgagacca agactttcca atagatgatt gaacacccct
    34021 ttatcaatat ttaggaaggc atctgtaaag aaactgatga taacccaaga caagaagtaa
    34081 gggaacaaca tagaagtttg gaaaatcttc accattctct tagaacggag ctcgctgagg
    34141 ataatggcaa tccctacaga tacaactaaa cctagaaaga taaagccaag attgtagagg
    34201 acggtatttc gtgtgataat aaaggcgtct cttgaactaa ataagaatct gaaattatcg
    34261 agtccgaccc atttactatt tatgatacta tctatgaaac cattactggt catgtggtag
    34321 tctttgaagg caaccacgtt cccaaatact ggaatgtaaa agaatagaat caaccagagt
    34381 gcccctggca aaaccatcaa gagaaagatc cagttgtctc tcaatgtttt tgaaaacttt
    34441 ttcataattt cctccctttt tattttgata tccatctaaa aattcttttt tagacttttg
    34501 ataacgatta cattattagt atactcctat ttgcaggtta ggttaaactc ctaattatag
    34561 aaaaaactcc acaaattatg tagcagattt aaaactttat caccactatc aaacaaatgt
    34621 cctaaatcaa ttgtttattt tatctctatt agcccagtga tggcgtcact ctgttataag
    34681 catccaacaa cggggtatac tgaaaaatct ccagactagg gaactcagcg atagttccta
    34741 atctggagat ttttaatatg ttattaggcg tttgctttca acttagcaat aacctcttta
    34801 agattatcaa tcacctctgc ttcagtatgc tcagagcctt tttcatctgc caagaacaaa
    34861 actgcttttt gaagttcttt ttgagagttt tcaaggacat ccttatctac tgtttcaagg
    34921 tttgagtctt taagaagttt acttaattcc ttggctaatt tcttgagttt gatttgcaga
    34981 ctcatcttct cctgctgttt ctttgcccgc tgtttgtcct ccatccttag ttgctgactg
    35041 gctttcctta atggactcta gggaagcaat ggcatctttg actgtttgca agatatcacg
    35101 taaaccttgc tctgtcaaac tatcatctgc aaaagctttc ttagcctctg ccaaaaccag
    35161 acgtgctgaa tctgtggtag gattcgatac acctgtcaat gatctcaaaa gattttctaa
    35221 ggtttgagtc tgcttactaa tactagacta aaatcaaaaa gcattatata acagtgatat
    35281 gaaatcaact aaagaagaaa tccaaaccat caaaacactt ttaaaagact ctcgtacagc
    35341 taaatatcat aaacgccttc aaatcgttct atagtaaaat gaaacaagaa caggacaaat
    35401 cgatcaggaa agtcaaatcg atttctaaca atgttttaga agtagaggtg tactattcta
    35461 gtttcaatcc attatatttc gtctgatggg caaatcttat aaagagatta tagagctttt
    35521 atagtgattt gaaataagat gtgaacaact ctatcaggaa agtcaaatta atttatagaa
    35581 atattttagc agccaaggtg tactgttata gattcaatac act
//
LOCUS       DAVKGU010000012        35508 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKGU010000012 DAVKGU010000000
VERSION     DAVKGU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.012"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            709..2724
                     /locus_tag="VW077_000550"
     CDS             709..2724
                     /locus_tag="VW077_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9454622.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFPRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEVWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLTTSTEWHDY
                     SPNGNVLIVTPHYLERQNIPVDTTIKQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            2726..3358
                     /locus_tag="VW077_000551"
     CDS             2726..3358
                     /locus_tag="VW077_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454623.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(4286..4699)
                     /gene="ndk"
                     /locus_tag="VW077_000552"
     CDS             complement(4286..4699)
                     /gene="ndk"
                     /locus_tag="VW077_000552"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9454624.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEESLPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(4810..8487)
                     /gene="rpoC"
                     /locus_tag="VW077_000553"
     CDS             complement(4810..8487)
                     /gene="rpoC"
                     /locus_tag="VW077_000553"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9454625.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYIHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLLYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(8520..12131)
                     /gene="rpoB"
                     /locus_tag="VW077_000554"
     CDS             complement(8520..12131)
                     /gene="rpoB"
                     /locus_tag="VW077_000554"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9454626.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            12547..12617
                     /locus_tag="VW077_000555"
     tRNA            12547..12617
                     /locus_tag="VW077_000555"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12579..12581,aa:Cys,seq:gca)
     gene            complement(12815..14146)
                     /locus_tag="VW077_000556"
     CDS             complement(12815..14146)
                     /locus_tag="VW077_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9454627.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(14437..15261)
                     /gene="endA"
                     /locus_tag="VW077_000557"
     CDS             complement(14437..15261)
                     /gene="endA"
                     /locus_tag="VW077_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9454628.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(15300..15488)
                     /locus_tag="VW077_000558"
     CDS             complement(15300..15488)
                     /locus_tag="VW077_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9454629.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(15481..16764)
                     /gene="murA"
                     /locus_tag="VW077_000559"
     CDS             complement(15481..16764)
                     /gene="murA"
                     /locus_tag="VW077_000559"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9454630.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKVSQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(16788..17018)
                     /locus_tag="VW077_000560"
     CDS             complement(16788..17018)
                     /locus_tag="VW077_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9454631.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(17076..18113)
                     /locus_tag="VW077_000561"
     CDS             complement(17076..18113)
                     /locus_tag="VW077_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9454632.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKEIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIDLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(18097..18585)
                     /gene="coaD"
                     /locus_tag="VW077_000562"
     CDS             complement(18097..18585)
                     /gene="coaD"
                     /locus_tag="VW077_000562"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9454633.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDKLVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(18575..19114)
                     /gene="rsmD"
                     /locus_tag="VW077_000563"
     CDS             complement(18575..19114)
                     /gene="rsmD"
                     /locus_tag="VW077_000563"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9454634.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(19179..20171)
                     /locus_tag="VW077_000564"
     CDS             complement(19179..20171)
                     /locus_tag="VW077_000564"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9454635.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(20283..20966)
                     /locus_tag="VW077_000565"
     CDS             complement(20283..20966)
                     /locus_tag="VW077_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9454636.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(21022..21762)
                     /locus_tag="VW077_000566"
     CDS             complement(21022..21762)
                     /locus_tag="VW077_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9454637.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFYLS"
     gene            21796..22074
                     /locus_tag="VW077_000567"
     CDS             21796..22074
                     /locus_tag="VW077_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9454638.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYYDFKIAN"
     gene            22152..23078
                     /gene="yidC"
                     /locus_tag="VW077_000568"
     CDS             22152..23078
                     /gene="yidC"
                     /locus_tag="VW077_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9454639.1"
                     /translation="MKSIKRFALSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(23260..24054)
                     /gene="pflA"
                     /locus_tag="VW077_000569"
     CDS             complement(23260..24054)
                     /gene="pflA"
                     /locus_tag="VW077_000569"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9454640.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(24180..25430)
                     /locus_tag="VW077_000570"
     CDS             complement(24180..25430)
                     /locus_tag="VW077_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9454641.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTPAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(25497..26324)
                     /gene="purR"
                     /locus_tag="VW077_000571"
     CDS             complement(25497..26324)
                     /gene="purR"
                     /locus_tag="VW077_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9454642.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(26591..27532)
                     /locus_tag="VW077_000572"
     CDS             complement(26591..27532)
                     /locus_tag="VW077_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9454643.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(27522..28778)
                     /locus_tag="VW077_000573"
     CDS             complement(27522..28778)
                     /locus_tag="VW077_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9454644.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(28780..29442)
                     /locus_tag="VW077_000574"
     CDS             complement(28780..29442)
                     /locus_tag="VW077_000574"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9454645.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAEV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(29405..30061)
                     /locus_tag="VW077_000575"
     CDS             complement(29405..30061)
                     /locus_tag="VW077_000575"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9454646.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(30072..30950)
                     /gene="rsgA"
                     /locus_tag="VW077_000576"
     CDS             complement(30072..30950)
                     /gene="rsgA"
                     /locus_tag="VW077_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9454647.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYRQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKLVPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(30952..31824)
                     /gene="rsmA"
                     /locus_tag="VW077_000577"
     CDS             complement(30952..31824)
                     /gene="rsmA"
                     /locus_tag="VW077_000577"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9454648.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(31955..32263)
                     /locus_tag="VW077_000578"
     CDS             complement(31955..32263)
                     /locus_tag="VW077_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454649.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(32256..32897)
                     /locus_tag="VW077_000579"
     CDS             complement(32256..32897)
                     /locus_tag="VW077_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454650.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(32899..34929)
                     /locus_tag="VW077_000580"
     CDS             complement(32899..34929)
                     /locus_tag="VW077_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9454651.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFIDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(35006..35302)
                     /locus_tag="VW077_000581"
     CDS             complement(35006..35302)
                     /locus_tag="VW077_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9454652.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
BASE COUNT        10139 a         8067 c         6957 g        10345 t
ORIGIN      
        1 aaaaaagtca aaaattacat aaaaatgctt gaccgaataa tatatatata ttaaaataaa
       61 aacaaggaaa aagaaaaggg tttcacgaat gaagaaaaat cgtggaattc aaaaattagc
      121 tatattagta ttactaggtg tttttatgtt tagtaacaca attccttacc aacagtttat
      181 tcagaagaat agacaattgg agattcgagt gcaatcacaa aagaagtcca atggtcttga
      241 tgttgtgaag gctgattgat ttcgtattac tttaaaattc aactatttag agaaaggtat
      301 tctgatgaga gcttgtaaca ataccagata gatataagaa tggagatgtt tgataaaatg
      361 ctttcatagc taaccaactg ttgaaaatta tgagttacaa aatctaaagt atttaagatt
      421 ggtttataaa attaggtctc tgcctgtaga aaggaacgtg cagttagaaa ggaggaaaaa
      481 ataggatact ataattcaga ctgagatttc taattaaaat atgtagttgc atttcgctca
      541 ttttcataat atttcattca gaggagtaat aatatgttaa aatttagatt tgatgcggga
      601 tggtaaagaa taattgaaga agattctttt atcaatagtt tagtaagctt gttagtttat
      661 agagggcagg ggtatttctg ccctctgttt tagaataagg aatgcactat gaaaaaatta
      721 tttatactca tatccaacct gcttgctagt ttgttctttg tttgggtatt taccatttgg
      781 actgatacat atgtctccta ttattacccc aacgttgttg tacatgattc ttcccctgaa
      841 acaacttttc agcatgttgc tacacgcttg gaaaaactag cagaagaaac agatagcttt
      901 attgcgattc aacaccaaga tcctaactca gaaggtacta cagttttttc ttatacgacc
      961 tttggggacg gaaagttgcc tgatgggctt caggaaaaaa atctagaaga tgctcaaagt
     1021 agtagtgttg aaacaaacta ttttgtattc gatggacacc tagatattca cttgctaagg
     1081 gaagagctaa gtcaacttgg cttgactaac atgcacctga caattccatc taaactatct
     1141 acgttaatgg ctatctttag taacggattt cagttaatca gtctattgat tttcatctta
     1201 acctttgtag cactgacttt aattagtcaa attagccaat tacggtcatc gggcattcgc
     1261 ctcatttcag gagagaaacg gtggtccatt ttccctagac cagttggtga ggatttgaaa
     1321 gggatagctg ttggtttcag tttagctggc gtgctcgcca ttcttatgca aaaaattctg
     1381 tcgcttccaa cgcaatccct aatgaccata ggagcaggct tgctcagtta taatctcatc
     1441 ttgttgtcga tctccctgtt tttcgctcaa ctcttcgcag ttgggataaa aaagatacac
     1501 ctgatgcaga ttataaaagg gcaagtgcct gtcagaggaa tcattagttt gattttaatc
     1561 ggtcaactgt tagcgattat tattgtaacg ctgggaatag ggagtagctt aaagtattcc
     1621 caagcctggc aacagcatcg gattggacaa gaggtttgga gtcaggaaag acaactgatt
     1681 accctatcaa tcagccgtga aggaacgagt cctggttttg atgaacaagc tcaaaggaaa
     1741 ctcagaactt ggtatcaatt gatggatctg gctgtttcag aacaaaaggc tttcttatct
     1801 agacaccagt taattgaccg tactttgcaa aatggcatgg cttcctccaa aaacttgaca
     1861 acctctacag aatggcacga ttacagcccg aatggcaatg tccttatcgt cacaccgcat
     1921 tacttggagc gtcaaaacat tcctgtagat acaactatta agcaaaagat gaatcacctt
     1981 aatgtagggg agtttgtctt attgctgcct gaacacctcc gttcagagga agaacattat
     2041 aaatctgttt ttgaagacga cttaaccagt cgcatgtcta gtcaagatga acgacagcaa
     2101 atgactgcta cggtaggtta tttagaatca ggtcaggatc gttttgtgta taatacgacc
     2161 cctatttctt accagcagtt tttgaaagat ccaatcatca ttgttataac accccaatca
     2221 actggtccac agtccatttt gttttggata gacgcagtac agaactacgt tctctttaat
     2281 caattgtctg atgcccagga gcttatccag agacaaggca ttgaaaattg ggtctcagaa
     2341 atgcaaacag gttaccacaa ctacatcaca ttattggata atatccagag ggaacgttgg
     2401 gtaatgctag caggagctgt gcttgggatt gcaacttcaa tcttgttgtt taacactatg
     2461 aataggctct actttgaaga atttagacgt gccattttta tcaaacgcat tgcaggtctc
     2521 aggttcttag aaatccatcg cacttatctc tttgctcaac tgggtgtgtt tttactggga
     2581 tttgttgcga gtgtatttct tcaggtagag ataggagttg ctttcttagt cttgttactc
     2641 tttactggtc tatctctttt acagttacat gtccaaatgc agaaagaaaa caagatgtcc
     2701 attcttgttt tgaagggagg ttaatatgat tgaacttaaa caggtgagta aatcttttgg
     2761 agaacgagag ttattttcga atctttcaat gacatttgag gctggaaaag tctatgcctt
     2821 aattggttca agtggtagcg gaaaaacaac cttgatgaac atgattggga aattagaacc
     2881 ttatgatggg acgatttttt accgaggtaa agacttggcc aattataaat cgagtgattt
     2941 tttccgtcac gaattgggct acctcttcca gaactttggc ttaattgaaa accaaagtat
     3001 tgaagaaaac cttaagctag gtctcattgg tcaaaagttg agtcggtcgg aacagcggtt
     3061 gaggcagaag caggctttag aacaggtcgg cctggcttat cttgacctag ataagcgcat
     3121 ctttgagtta tcgggcggag aatcgcaacg ggttgccttg gcaaaaatta tcttaaagaa
     3181 tccacccttt attctggcag atgagccaac agcttcaata gacccagcaa cctctcagtt
     3241 gattatggag attttgctat ctcttcgaga tgataatagg ctaatcatta tcgcaacaca
     3301 taatccggca atttgggaga tggctgatga agtgttcacg atggatcatc tgaaataaaa
     3361 atccttgttt ttaattgcac gatgagttac tgaaatatta tcatgaatca ataattggag
     3421 ttaatttaga attgtacttt attaatattg aggtaacttt ttcttgataa aggaagaaat
     3481 aatggagagg aagttagaat gaaaaaattc gacaattata ttattgagaa gccttgcgat
     3541 tctaattcag ataaactgca aaaaatctta ataattgaaa gtttggtaga tgatattttg
     3601 caattttctc tcagaatcaa taatagtgta ggagagattt tcctcctaca accgttttaa
     3661 aagaaaacta tctttattcc atgttatttt gaggaagata ttgtgaaagt caaagatgat
     3721 gataaagttg agtggaattt gttagaattt caaaaattta gagcattttt ggcttagtaa
     3781 tctgtgttga aggctcaaaa cctatggtaa aaaagtcgct ttgaaaacgt attgcctcca
     3841 aagatttagt taaataatga tttaacacaa aaagaaatta ttgaagttct ggaaagatgt
     3901 tgtttcagta ttgagaaaag gtgggaaaaa cttgcgattt tcacagagaa aggaagaaaa
     3961 agtatagaaa tatagtcaat tgaaacaaga acaggacaaa agaacctttt gtgccatatt
     4021 tttctccttt cgctttacaa ttggcttgaa cacctttatt gtatcgcgtt tggagttttt
     4081 ttggtataac cttcgacgcg cacccgcata gcgggtgttt ttttgtctcg cacctaacgg
     4141 agcgagacga actaatagtc acttaatcaa aaaacgcacc atatcaaaaa ctaaaaagtt
     4201 tgatatcatg cgtcatgtct taaactaatt gactatactt tctattcaaa tgagctttta
     4261 accaattgat tgagccaatc cactcttaaa accaaagagc aatttctcgc ttagctgact
     4321 cttctgaatc tgaaccatgt acaacatttt ggataacctc attttctcca gcagcttttg
     4381 caaagtcacc tcgaatagtg cctggtaaag attcttctgg acgagttgca cccatcatgg
     4441 tccgccaagt ttcgattact ttgggaccag aaatgacacc cacaagaact ggacctgaag
     4501 tcatgaattc acgaatcggt aggtaaaaac tctgaccaac caagtcctga tagtgctggt
     4561 caatcaactc ttctgaaacc tgtgaacgaa actccaattt ttcgattgta aatccacgtt
     4621 gttcgatgcg ctttaacact tcacccacta gccctctttt tacaccatct ggtttgatga
     4681 taaagaatgt ttgttccata cccgtctcct ttgtcagctt ctttctttta ttttaccaca
     4741 tttcgtggaa aaatggagaa agttttcaga agagagaatg agagaacccg agggttctct
     4801 cattctctct tattctactg tttcttccac agtttcaacg gcagtatcca caactacttc
     4861 tgttgtttct tcatttcctt cttcctctac tggaggatta aggtattctt cttcgttgac
     4921 agcatgtggt tcaaggttac ggtaacgggc cataccagta ccagctggga tgatcttacc
     4981 gatgataaca ttttctttaa gtccaaggag atggtctttc ttaccacgga tagctgcgtc
     5041 agtaaggaca cgagttgttt cttggaagga agctgctgac aagaaactgt ttgtttcaag
     5101 tgaggctttg gtaattccca taaggactgg gcgacctgtc gctggaactc cacctgcgat
     5161 aaggacatct ttgttggcat ctgtaaagtc attgatatcc atgagggtac ccatgagaag
     5221 atctgtatca cctggatcca tgacacggac tttacggatc atttgacgaa ccattacctc
     5281 gatgtgtttg tcaccgattt ctaccccttg gctacggtaa actttttgta cttcaccgag
     5341 aaggtacgtt tcaactgaca agacatcacg aactgcaagg agacgttttg gttggataga
     5401 accttctgtc agagcagcac cacgcgctac ttggccccca acttcgacac gcatacgagc
     5461 tgtaaatgga acgacatatt caccttcgcc agtttcaccc ttaacaaaga ctttcttggt
     5521 acgagttgat gcatcttctt cgatagcagt aacttgtcct ttaacctctg tgataaccgc
     5581 ttccccttta ggattacggg cttcaaagat ttcttggaca cgaggaagac cctgagtgat
     5641 atcggtattt gaggcaaccc cacctgtgtg gaaggtacgc attgtaagct gtgtaccagg
     5701 ttccccgata gattgggcag caattgtacc aactgcttca ccaacttcaa ccgcatcacc
     5761 agtcgccaag ttgataccgt aacagtgacg gcagacaccg tgacgagtgt tacatgtaaa
     5821 tacagaacgg atagtcactt cttccacacc agcattgaca atttcacgcg ccttgtcttc
     5881 tgtaatcaat tcatttggac caataatcac tgcaccagtt tctggatgtt taacagtttt
     5941 cttagtgtaa cgaccgttga gacgctcttc gagagactcg atcatctctt ttccttctgc
     6001 gatagaacgg atcaagagac cacggtcagt tccacagtcg tcctcacgga tgataacgtc
     6061 ttgggcaacg tcgaccaaac gacgagtcaa gtaacctgag tcggctgtct taagggccgt
     6121 atcggtcata cctttacgag caccgtgagt tgagaagaac atttccaata ccgacaaacc
     6181 ttcgcggaag tttgaaagga ttggcaattc catgatacgt ccattcggag cagccatcag
     6241 accacgcata ccggcaagct gtgagaagtt tgagatgtta ccacgggctc cagagtccat
     6301 catcataacg attgggttct taggatcttg gttagcaatc aagcgtttct caagtttttc
     6361 acgggcagca cgccattcag ctgtaacagc attgtaacgc tcgtcgtctg tgatcatacc
     6421 acgacggaat tgtttggtga tttgttcgac acgtttgtgt gattcttcaa tgatttcagt
     6481 cttgtcatca acgactggga tatcggcaat acccactgtc aatcctgcaa gagttgagtg
     6541 gtggtaaccg aggttcttca tgcggtcaag tagggcagaa gtttctgtcg tacggaaacg
     6601 tttgaagatt tcagcgatga tatttccaag gtttttcttc ttgaatggag ggttgagctc
     6661 aagattgctg atagcttcct tgatatctcc accaagtggc aagaagtatt tagctggaac
     6721 accttctgtc aagttggcat tgtttggttc ttgcaagtat agtagcccct ctggcatgat
     6781 atcgttgaag agaattttac caactgttgt aagcaagacc ttatgtcttt gctcttctgt
     6841 ccaaggcttg ttgaggctgt ctgttgcgat accaacacgt gagtggaggt gaatataacc
     6901 attgcggtaa gccataaccg cttcgtcacg gtctttgaag accattcctt caccttcgcg
     6961 accagcttct tccatggtca agtagtagtt acccaaaacc atgtcctgag atggagtaac
     7021 taccggtttc ccatctttcg ggttcaagat gtgctcagca gctagcatga ggatacgagc
     7081 ttctgcttgt gcttcttctg aaagtggtac gtggatggcc atttggtccc cgtcaaagtc
     7141 agcattgtag gcttcacaga caagtgggtg caagcgaaga gccttaccat caatcaagac
     7201 tggctcgaag gcttggatac ccaaacggtg aagggtcggt gcgcggttca aaagcactgg
     7261 gtgttcttta atcacttctt caaggatatc ccagatacgc tcatctccgc gttccaccaa
     7321 gcgtttagct gctttgacgt tttgcacgat atcacgggca acgatttcac gcatgacaaa
     7381 tggtttaaag agttcaatcg ccatttcacg cggcacacca cattggtaca tcttaagagt
     7441 tggaccaacg gcgataacgg aacgtcctga gaagtcaaca cgtttaccga gcaagttttg
     7501 acggaagcgt ccctgtttac ctttaagcat gtggctcaat gatttcaatg gacggctacc
     7561 tggtcctgtg attggacgac cacgacgacc attgtcaatc aaagcgtcaa ctgcttcttg
     7621 aagcatacgc ttctcatttt gaacgatgat acctggggca tttaactcaa gcaaacgagc
     7681 caaacggttg ttacggttga taacacggcg gtaaaggtca ttcaagtcag atgaggcaaa
     7741 acggccacca tccaactgca acattggacg aagatctggt gggataaccg gaaggatgtt
     7801 aagaatcatc cattcaggtt tgtttccaga cttgtaaaag gcatccaaaa catccaaacg
     7861 acggatggct ttgacacgtt tttgtccagt agctgttttc aattcttctt tgagttcagc
     7921 aatttctttt tcaagatcta cttgcttcaa aaggtcttgg atggcttccg cacccatctt
     7981 ggcaacaaat gaaccataac catattcacg caagcgctct cggtattcgc gctctgtcat
     8041 gatagacttg tgctcaagtg gtgtatcctt aggatcaatc accacataag ccgcaaagta
     8101 gataacttcc tcgagggcac gagggctcat atcaagggtc aagcccatac ggcttggaat
     8161 ccccttgaag taccagatgt gagatacagg agctttcaac tcgatatgtc ccatacgctc
     8221 acgacgaact ttcgtacgcg ttacttcaac cccacagcgg tcacaaacaa ttcctctgta
     8281 acgaatgcgt ttgtacttac cacaagcaca ttcccagtct tttgtaggac caaagatcac
     8341 ttcatcaaag agtccttcac gttctggttt caaggtacgg taattgattg tttcaggttt
     8401 tttgacttct ccataagacc atgaacggac tttacttgga gaagctaggg tgatttgcat
     8461 acttttaaaa cgatttacat caaccactat ttcttccctt tctattctaa gtgaactgct
     8521 tattcctgtt cagcagcttc ttctgttgct tccgcttttg ttgctttctc agcttcttca
     8581 gcttcaaagg ctgctttagc ctcttgggct gctttttcgc gggctttttc aaggtcatct
     8641 acgtggatga catcttcgtc cattccttca tccaagtcgc gaagttccac ttcttggtca
     8701 tcttcgtcta gaacacgcat gtcaagacca agagattgca attctttgac aagaactcgg
     8761 aaggattctg gaacacctgg ttttggaatt ggtttgcctt ttgtaatagc ttcataggct
     8821 ttcaaacgtc cgttgatatc gtccgacttg taagtcaaga tttcttgaag gacatttgac
     8881 gcaccgtagg cttcaagagc ccaaacctcc atctcaccga aacgttgtcc accaaactga
     8941 gctttacctc cgagtggttg ttgggtaaca gttgagtaag gtccgactga acgcgcgtgc
     9001 aatttatcat caaccatgtg gtggagtttg atcatgtaca tgactccaac agaaacacgg
     9061 ttatcaaatg gttcacctgt acgtccatcg taaaggattg tcttggcatc gctatccata
     9121 cctgcttctt taacagttga ccaaagatct tcagaacttg ctccatcaaa gactggtgtc
     9181 gcaatgtgaa taccaagagt acgagctgcc ataccaaggt gaagctccat aacctgaccg
     9241 atattcatac gtgatggcac cccaagtggg ttcaacatga tgtcgactgg agttccgtct
     9301 ggaaggtaag gcatgtcttc tacaggaacg atacgagaga caaccccttt gtttccgtga
     9361 cgtccggcca ttttatctcc gaccttaatc ttacgttttt gagcgatgta aacacgaacc
     9421 aacatgttaa cacctgattg caactcatct ccatttacac gtgtaaagat cttaacatca
     9481 cgaacgacac catcggcacc gtgtggtaca cgaagagaag tatcacgcac ttcacgagac
     9541 ttgtctccaa agatagcgtg caagagacgt tcttcagctg aaagatcttt ctcaccctta
     9601 ggtgttactt tacctacaag aatatcacct tctttaacct cagcaccaat acggataatc
     9661 cccatttcgt caaggtcttt gagggcatct tcaccaacgt ttggaatttc gcgagtgatt
     9721 tcttcaggcc caagctttgt atcgcgcgtt tctgattcgt attcttcaag gtgaacagat
     9781 gtgtagacat cgtccttcac caagcgttcg ctcatgataa cggcatcctc gaagttgtaa
     9841 ccttcccaag tcatgtaggc aacgattggg ttttgtccaa gcgccatttc tccattttcc
     9901 atagaaggtc cgtcagcgat gaaatcgcct ttttcaacga catcaccaac ttttacgaga
     9961 gtgcgttggt tgtaagcagt acctgagttt gaacgacgga atttttggat gtggtaaaca
    10021 tccaatgaac catcttcacg acgaacttct accttgtcag catctgcgta agtaacttta
    10081 ccatcatact gagcaatcac agccgcacca gaatcgtggg ctgcttggta ttccatacca
    10141 gtaccaacgt aaggtgcctg aggattaatc aatggcacag cctgacgttg catattggct
    10201 cccatgaggg cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg
    10261 gcaactacct gttttggtga aacgtccatg tagtcaacaa tattagctgg atactcttgg
    10321 ttgacccctt ggtgacgtcc catgacaatc ttctcagcaa aggttccatc ttcattcaga
    10381 cgagagttag cctgagctac agtatattca tcttcttcat cagctgtcaa ccaaacaatt
    10441 tcgttcgtga caacacctgt ttcacggtca accttacggt atggtgtttg aacaaaacca
    10501 tatttgttca agtgtccgta agatgacaag ttattgatca aaccgatgtt aggtccttca
    10561 ggtgtctcga ttggacacat acgaccatag tgagtgtagt gcacgtcacg cacttcatat
    10621 ccggcacggt cacgagtcaa accaccaggt cctaaggctg acaaacggcg tttgtgagac
    10681 aactcagaaa gcgggttgtg ttggtccatg aactgtgaca actgtgatga accaaagaat
    10741 tctttaactg cagctgttac aggacgaata ttgataattt gttgtggtgt caagacttca
    10801 ttgtcctgaa cagacatacg ttcacggaca ttacgttcca tacgagaaag tcccaaacgt
    10861 acttggttgg caagcaattc accaaccgca cggatacgac gatttccaag gtggtcgata
    10921 tcatctacac ggccaagtcc ttcagccaag ttgaggaagt agctcatctc agcaaggata
    10981 tctgcaggag tcaccgtacg aaccttgtca tctgggttag cattaccaat gatcgttacg
    11041 acgcgatctg gatcagttgg agcaataacc ttgaattttt gaagaacaac aggctcagtc
    11101 acaacggctg catcgtttgg gatgtagaca atcttgttca agtcgccatc caaatggctt
    11161 tcaatgcttt caatcacgct acgagtcata atcgtaccag cttctaccaa gatttctcca
    11221 gtttcagggt ctaccaatgg ctctgcaatg gtttggttga gcaaacgtgt tttaacattg
    11281 agttttttat tgattttgta acgaccaact gctgccaagt cataacgacg tgggtcaaag
    11341 aagcgagcta caagcaagct acgtgagctt tcagccgtct taggctcacc tggacgaagg
    11401 cgttcgtaaa tttctttcaa ggcttcgtct gtacgagagt ccattggatt cttgtggata
    11461 tctttttcaa cagtgttgcg aaccaattcg ctgtctccaa agatatcaaa gatttcatca
    11521 tcacctgaga aaccaagagc acgaaccaag gttgtaaatg gaatcttacg agtacggtcg
    11581 atacgagtgt aggtgatatc ttttgagtcg ctttcaagtt ccaaccaagc tccacggtta
    11641 gggataacag ttgaaccata gcccacctta ccatttttgt ctactttgtc gttaaagtaa
    11701 acacctggtg agcggaccaa ctgagaaacg ataatacgtt caccaccatt gatgatgaaa
    11761 gtacccattt ctgtcatgat tgggaaatca ccaaagaaaa cttcttgggt cttgatttcg
    11821 cctgtttctt tattgatcaa acggaaggtt acaaaaattg gtgctgagta gctagcatcg
    11881 tggatacgag cttcttctag cgtgtatttt ggttccttga tttcatatcc aacaaattcc
    11941 aactccattg tgtctgtgaa gtttgaaatt ggcaatacat cttcaaacac ttccttaaga
    12001 ccgtggtcta ggaaagcttt gaatgagtca gtttgaattt caatcaaatt tggtaagtca
    12061 agaacttctt tgattcttga aaaactacga cgggtacgat gtttcccgta ttgaacgtca
    12121 tgtcctgcca agatgattct cctttgtaaa taagttccaa gccttgtcaa tcaggctttt
    12181 ctaatcgtca tatggttgta aaccccttat caccgtgtcc tcttgacgaa ttttcagaat
    12241 ctttaagcct ctgttacaaa tgctcataat cttgaaaaaa agcacaaaaa gagcagctaa
    12301 atctgacttt ttcagaagat ttaactgctg tgagccttgt ctggacaata tttcagacaa
    12361 aacctacgac aaatgattac ccatattata ccctatttag ctagattttt caaggggttt
    12421 cagtaggttt ttggtaaatt ttttcccata gaaaacttgg catcacattc gaatcacgct
    12481 atggtacaaa aaactgaaaa aactattgac tgaaaatcat tttcaaggta taataataaa
    12541 cgttaaggcg gtatagccaa gtggtaaggc acggctctgc aaaagcttga tcgtcggttc
    12601 aaatccgtct accgccttct ataacttgat ttatcaggtt tcaaatgaac agaaagccca
    12661 atttgaaggg ctttttttat tttccctcga ataaatacgt ataactttaa aaacttttgg
    12721 agcgagtttg tggcagagtt ctttccatgg cataattccc ttttgaaatc aattgaaaaa
    12781 agcttcgtca cgcctggtga caaaagcctc tagtttattc tgtttcttct tctatttcga
    12841 cttctgtgat ttggactttt accttggtaa cacgtccatt cttcacttta tcattggtta
    12901 agataaactg tttgttttgg ctgaccaatt catagctgag tttctcagtc gttggaatgg
    12961 ttcccactcc tgtcaaataa taaccagcga tggtatcaac gtcatcactt tccagctcaa
    13021 catcaaagta attattgaaa tcattgaggg tcatagttcc ttgaacaatg taggtatcct
    13081 cgccaatctg atgaacatct attgctgctt tgtccgtttc atcatcaatt tcaccaacaa
    13141 tttcctctaa gaggtcttcc aaagtcacta aaccagccat accaccgtat tcatccagca
    13201 agattgccat ttgtctttgg gtatttcgca gttcttttag caagtcatcc acaaaaatag
    13261 tttcaggtac aaaaagtgga tcttgtaaaa ttctcttcca aacaatattg tcaaaaccgt
    13321 ccacaaagcc tgccttaagg agactcttgg tgtgaatgat tccaattaca ttgtccttat
    13381 ccccatcata aaccgggata cgagaataat tttgttttaa aatactttgg ataatggctt
    13441 gactatcatc ctgaatatcc accataaagg catctgttcg aggaaccata acctctcgtg
    13501 ccatcagttc atcgagcgaa aagacacctt gtagcatctc aatctcatca gcatccaatg
    13561 tttcttcact atttgtcagc atataggcaa tttcatcacg ggtcattttt tcatccgcat
    13621 catcgaatga cataggagtc aaatggctca agaaattggt cgaagcagct aaaagccaaa
    13681 caaaaggact gactagtttt ccgatcccaa tgataaccgg cgctgtacga attgccaagg
    13741 catcctttag attaagagcg attctcttag gatataattc cccaaaaacg atggaaatat
    13801 aggtcaaaaa tgccaaggat agaaaagttg ccacggcttg tgctgtttcg ccattcccaa
    13861 gccaagaggc aatcacacgt cctagagtat cagttaaact cgcccctgat aagattgtaa
    13921 tcagggtgat tcctacctga atggttgata aaaagtggtt aggattttct agtaccttca
    13981 gcaggcggat gtagcgtctg tctccttctt ccgccttttg ttcaactcgg gcacgattaa
    14041 gagaaaccat ggccatttct gtggctgaga aaaaggcatt taataccgtc aagataaaca
    14101 ataaaacaaa ttgtagcaac aaattctgac tacttgggtc ttccatagtt cttctcctaa
    14161 aatagtatct tatcccttat tatatcacaa aatttaatcc aatacatatt atttttttct
    14221 tagcgtaact tccatctctc tcccaaaact ggaagttagg ctaagacgga tttccactga
    14281 tttcacgcgt ttttctcata ctaaaagcta ggttgtggga aactggaagt tagttttaga
    14341 agttactatt ttttcttagt acctgttcta accctgctta aactagtaaa aagaactcct
    14401 acatccataa gtgacatagg agtttaggct tatcttttac tgagttacag ttacttctcc
    14461 agttcggtaa tccagttgaa gtcccttttg aacattggga actagaacat tgaattccaa
    14521 ttctccatcc gaagacttgg cttcaatctg tgaagctgag ggaactaaat cctcgtttga
    14581 agcgtagtaa agggttacac ggtaacggac acgcttgttt tggtccaagg ctttacgcac
    14641 cttgctttca tagtagtttt gaccagtcga atactcggct tgtgcctgat ttgcccaggc
    14701 tgtctgaaca gcaatgtttt taggattgct tgttgaggca tcaaaaccat ccaaaccacc
    14761 gattaaggca tagcctaaca aatgacctct atcgactgca tgggtataag agccctttag
    14821 attcttgacc tgatgccaac ctggaggagt ccaagaagtt gaaccattcc cagtttcttt
    14881 acgattcttg tactgacgag tggccttaga caagagggca ttagctacgg ttggaacagt
    14941 ttccttgccc actgtctttg ttttattgtc agcgtagggc ttacttgaaa ccttggcatc
    15001 tagatttgtt ttattaccat tgacgataaa agcacctgag ccattccact ccagactccc
    15061 ctttatttga ctcttgactg cgtctgttaa gacactctct gccaatgctt gactaggagc
    15121 ttcagacgct tgttttttct gactaagctt ggttttagga ctattagctg tcgactgcat
    15181 ctgcttgata taatagctcc ctgtagacaa aagcaataac actagcagtc cgattagtgt
    15241 ctgtcttgtt tttttgttca tatttctcct tatctttaga aaaaggctgg ttctccagcc
    15301 taatttcctg taaatttatg aatcaattcc tgccattttg ctggagacag gatagcccat
    15361 ggatcttgac ccttgcccaa gattccataa cctaccatta aaccgattcc tagagccaga
    15421 gtacctaaaa tcagtactat gatgactaaa agtaaacgct tgactacgta gcttgatttc
    15481 ttattcatct tcatcacttg cctcaatccg ctgaatctta gcacctagct gcgccaactt
    15541 ctcatggaaa ccgtagtaac ctctatccaa gtgaaccaat ttaccgacca cagtttctcc
    15601 ctgtgctacc aaacctgtca aaatcaaggc cgcactggca cgaaggtcag ttgaaagaac
    15661 ttctgctccc tgcaaaggct gtccaccaac aatacgagct gtatcacgga taatctcaga
    15721 atgcaagccc atgcggcgca tctcttctag gtgttggaaa cgattttcaa aaactgtctc
    15781 caccatggtt gattcgcctt ttgcaactgt catcaaggct gtaaattgag cctgcatatc
    15841 tgttggaaat cctgggtggg gcaaggtttt cacatgaaca gcttttagat tttccagttg
    15901 agaacgaaca cgaattcctt cgtcttcttc aattacttca acacccattt caagtaactt
    15961 ggcaatcaag ggacggttgt gctcccagac agcgtctcga atcaagacat caccaccagt
    16021 catggcagca gctaccataa aggttcctgc ttcgatacgg tcctggacta cattgtgagt
    16081 cgtaccatga agtttctcaa caccagtaat ggttatagtc tctgtaccag cacctttgac
    16141 cttggctccc atttcattaa ggagaatggc taagtcaaca atctcaggct cacgcgcagc
    16201 attctcaatc actgtcaccc catcagccag agtcgctgcc atcatcaagt tctgcgttgc
    16261 accaacactt ggaaagtcca tatagatatg agcgccatgc aagcgttctg ccttggcttc
    16321 gatgtaacca gctgtctgac taaccttaac ccccatagct tccagacctt tcaaatgaag
    16381 atcaatagga cggctaccaa tcgtacaacc acctggcatg gataccttgg catgacccac
    16441 acgggcaagg attggcccta atacaacgat ggaggcgcgc atcttgctga catacttgta
    16501 aggggcttcc tcagtgatgt cgccagtagc atccaccttg acaagatgag cttcctcatc
    16561 aaagtcaacc ttggcattca aaccaccaac tacctgattc ataataaaga catccgacaa
    16621 aatcggaaca ttctgcaaga cggtctttcc ttcacttgct agaatagtcg ctgccaacaa
    16681 gggtaagact gcattttttg ctccctcgat cgtcacgctt cctaccagac gattatcgcc
    16741 accttgaacc acaattttat ccatactcgt ttcctttact cttgatttta taataatcct
    16801 ctaagcgctt cttgccacaa atgatacagg ctaataaaga aagaactcag gatgtagcct
    16861 atgacaatgc taaaaaaggc tactaataaa cgaacttttc ttgtattctc agctgtcact
    16921 tttaaaacct tttcccatct aacaagatcc tttaaaaggt aaaaactcac ataaataaag
    16981 agcatatgac tacttagagt gaataataat tgaaccattt gactattata ccatcttctc
    17041 tgtttgtgcg ctagtttgga aacttcactc aaaaattagg gattgttttt caagtaatca
    17101 atcgcatctt gtagggtttt aacgggcacg attttcatat ccgtcttgat tgttttagcc
    17161 gcttctaggg ctgtttggta gttgtttttc gcgtccggat gcgccttttg ttcttcttcg
    17221 ctaacagggt tatcaggagc aaagaaaata gcagcacctg ccctagccga agctacaacc
    17281 ttcttatcaa tacctccaat gtctcccaca ttaccatcgc ggtcaatggt acctgtaccg
    17341 gcaacaatac gaccattacg aagatctggg tgagctattt gagtatagat agctagacta
    17401 aacatgagac cagcacttgg accgccaata ccagctgttg aaaagctaat tgggacattg
    17461 ctgattacct ctgtacggtc aatcaagtcg attccaattc catttttgcc attttccaag
    17521 gtgataatct ttccttctgc agacttggtt tgcccatcct cttcataggt gaccttgacg
    17581 gagtccccta atttttgaga actgacgtaa tcaatcaagt ctttggaact atcaaaggtc
    17641 tgatcattga ctgctgtgac tgtatcagag atattgagaa tccctttaaa ggttgaatta
    17701 tccgtcacat tcaaaacata aactccaaag tacttgagtt cgatttcctt accagctgtt
    17761 tttagtcctt gatacttggc catattttgc gatgtttgca tgtagaattg attgattcgc
    17821 ataaattcaa catcggaaga accacctgta gtctcctgag cactacgaat atctgtaaaa
    17881 ggtgtcaacc aagcataaat catatgagct aaagtagcat gttgaacacc aaccgtaacg
    17941 aattgatagg caccagcttc cttatcttct gtgtcattta ctttaaggac ttggcgaata
    18001 tcttccgaac cacctggaac ctctatatag tagggcaaag gcaccacaaa tgccaagaag
    18061 gtcacaatca aggccgcaat gacatacaaa ggccatctaa tctttttttt catttcttat
    18121 ttcctccaaa atactctcgg gaacatggca ggcaatatcc tgaccaaact tcaaaagctc
    18181 tcgaacacct gatgaactga tatagagatg ttcaggccgg ctatgtaaat aaatagtctc
    18241 tatatcagaa gacagctgat gattgtagta atcaaaacta gcttcatatt gcaaatccgc
    18301 cgcattcctc aacccccgta ctagaaaagt agcccccagt ctttttgcaa catcgaccac
    18361 caatttatca tgagaagcca cgacttcaac attttccaaa tgtcccaaag ccttttctag
    18421 cccccgttta cgattttcaa gagggagaaa tccttgtttg tggggattaa aaaaaatacc
    18481 cacataaagc ttatcaaaaa gtctgctcgc ccgttcaatg atatccagat gcccatttgt
    18541 catcggatca aatgagcctg tgaataagcc aatcttatct gacatagact gtcaccttac
    18601 taattccata aatcttttcc ttccagatac ccagacaggc aatttcttct ggaagttcaa
    18661 cggctttatc cgtctcgcac acaaccataa catcttcaga aaaaagctct ctctcagcca
    18721 ttttttcaat atctgctacg atttgttcct tggcataggg agggtctaag aaaacgaggt
    18781 caaattcccc agatacctgt tccaatgccc tttctgcatc catctttagg agttgaaatt
    18841 ttccaacttc cttggtcatc tggatatttt cagccacgat ggtctgagcc ttacggtctc
    18901 gctccaccaa aacagcactg gacatgccac gcgatactgc ttcgatagat aaaccaccac
    18961 tacctgcata aaggtccaag actcgtccca cttcaaagta gggaccaatc atgttaaaaa
    19021 tggctcccct aaccttatcc gaagtaggtc ttgtcgtctt gccttctagt gtcttgaggg
    19081 gacgtccccc atagattcct gatacgattt tcatactgtt tattatacca aattatagac
    19141 aaaaagagaa agaaaaccga accttgcggt tcgattctct acaaaatatt ttcgtaagta
    19201 tcgcggactt cttgaggcca aacacttgtt tgcacttctc cgatgtgtct cttgcgaagt
    19261 aggaacatgg ccatacgaga ttgtccaatt cctccaccga ttgtcaatgg gaatagacca
    19321 ttcaacaaag acttgtgcca ttccaattcc aagcggtctt catcacctgt aatttccacc
    19381 tgacgtctaa gagtttcttc atctacacga attcccatag aagacaactc aaaggctcca
    19441 cctaaagact cattccagac aagaatatca ccatttagac ccttgtagcc attctcagac
    19501 tcgcttgtcc agtcatcata gtctggtgca cgtccatcgt gcggtttacc atctggcaac
    19561 tcgccaccga taccaatcaa aaagacggct ccaaattctt tacaaatcgc attttcacgt
    19621 tctttcggtg tcaagtctgg gtagcgttct accaattctt ctgtatggat aaaggtgatt
    19681 tgttttggca agatagactc gatgtcatag cgggcttcaa cagctagctc agtcaggcga
    19741 atagccttat aaatcttctc aactgtttct tttagataaa cgatgttacg cttaccattt
    19801 gggataacct tctcccagtc ccactggtca acataaacag agtgggttgc atccaaggaa
    19861 tcctcatctg gacgaagggc tttcatgtgg acaaagagac cctctccttc accaaagcca
    19921 aaacgagcca aggtgtggcg tttccattta gcaagtgagt gcaccacttc ataagtagca
    19981 tcagggattt ggagaacctt gaccgatact ggattttcca caccagacag gttgtcctgc
    20041 attccgtcac cgaccttact caagatagga ccttgaactt cgacaacttc tagcttatct
    20101 ttcaaatact gggtaaaagt gtttttgaca aaggaaattt cttcttgttg atggataaaa
    20161 cttttcttca tagacaactc ctcaaaaatt taataaacgt attatacacc tatttccaag
    20221 aaaagaaaag agaaaattta aaaaaaatca gtgtcactca aaacactgat ttcatagacc
    20281 ttttagtcat tgcgaccaaa gatacgtaga atacttagga aaagattgat aaaatcaaga
    20341 tagatgctga gagccattga caccacccag cctgtcgcta cacgaccttg tgattgttca
    20401 taggcgaggc gaatcttttg gttatcccaa gcaatcaacc ctgaaaaaac caaaaccatg
    20461 gctacgctaa tcatataatc aaagaagcca ctagccaaga aaatattgac taccatagca
    20521 atcagtaaac caatgagagc tgccatcatt gctcgaccaa tcccactcaa gtctttcttg
    20581 gtaaagatac caactgccgc catgacaaag aagagaaggg cgctcgatac aaaggcagat
    20641 aaaactgtac ccggagtata gaaagccacc acaaaactaa gggtaaagcc gtttaaaacg
    20701 gagtaaagta aaaatactgg aagagcagct ggactatttc ttgaagccat gctactggca
    20761 acgaaaacca aagccaactc cgcaaaagta gcaatggtca accagagacg cccctgcatc
    20821 aaaaagtaaa ccaactgaga ctgaaaaacc gtcaacataa ggcctgatac caaagcagat
    20881 agtccgattc ccagaccaac aaaggcataa accttagcgt aaaattgatt gagacctgca
    20941 cggtcatgta taatagtgtg attcatccat ttctcctttt ctgtgaacat ctttccttga
    21001 ttataacaaa aaaagttaaa attagcttaa atagaagatt aaaataccgg ctgcaaccgc
    21061 aacattcaaa ctctcggcct gccccttcat gctaatatgg accaactggt ctgcactttc
    21121 agccataagg ggactaattc cttgaccctc atttcccata actagtacaa aattttctat
    21181 agaaggcagt tctctgtaat caacagaatc tttagatagg gttgttgcta gcactgggat
    21241 agctaccttt ttagtttcgt caagaagcgc ttgactagtc atccggtaaa tgggcagatg
    21301 gaaatgacta ccttgcatgg aacgtagggt tttgagactg tagatgtctg ccgacttatc
    21361 tgaaacaatc actcctgtaa aacctgctgc atccgcagtt cgaatgatag ttcctacatt
    21421 accaggatct tgcacatctt ccaaaaacaa gaacttgccc tgactcaaat cagcttgtcc
    21481 tacttcttct ttttgaacca cggcaacaat tccctgtgga gtctgagaat ccgccaaatc
    21541 tagcaaaata tcctctgaaa tccagacagt ttgcggaaaa gcagctaact gatttcgata
    21601 attttctagg gcaaagattt tctcaatcgt cactccagct tggacagctt cttcaaacaa
    21661 gtgccagcct tcaatcaaat aggcagactt gcggtatttt ttttggtgta atttcttggc
    21721 attttttacc acagaattgg cttttgaggt tataatagtc atagaaatat tataacacaa
    21781 tcaaaggggt ttggtatgca aaaggttaga atgattgccc aaggtagggt acagggagtc
    21841 ggctttcgtt ggggtgttta cagcttggca cttgaaattg gtggcatcac aggtcgagta
    21901 tggaataacg acgatggcac agtggaaatc ttagcccaag cagactcatc tgctatcatg
    21961 gcaaaattta tccaagaaat ccgaaaagga ccgacacctt tttcaaaagt aagctactta
    22021 gatgtcaaac taagcaactt tcctccctac tatgacttta aaatcgcaaa ttaggtctcc
    22081 agaactattg tatattttgt aaaaaaacag tagaatagaa aggtataatt tttaaagaag
    22141 gaataaaaac agtgaaatct attaaacgtt ttgcactctc ggctatggga gtggctatgt
    22201 tgcttgtctt gactggctgt gtcaatgtcg ataaaaccac aggtcagcca acaggattta
    22261 tttggaatac gatcggagcg cctatggctg aagctatcaa gtacttcgct actgataaag
    22321 gtctaggctt tggtgtcgct atcatcatcg taaccattat cgtgcgcttg attatcttgc
    22381 cacttggtat ctaccaatca tggaaggcaa cgcttcactc tgaaaagatg aacgctctca
    22441 agcatgtcct tgagccacac caaacacgtc tcaaagaagc aactactcaa gaagaaaaac
    22501 tcgaagccca acaagccctc tttgccgctc aaaaagagca cggcatcagc atgtttggcg
    22561 gtgtaggatg tttccctatc ctccttcaaa tgcctttctt ctctgctatc tactttgctg
    22621 cccaacatac tgaaggggtt gctcaagcaa gctacctagg cattcctcta ggttctccaa
    22681 gtatgatttt ggttgcctgt gctggtgtcc tttactatct tcaatcgctc ctttcacttc
    22741 acggagtaga agatgaaatg caaagagaac aaatcaagaa aatgatttac atgagcccac
    22801 tcatgatcgt cgtcttctcc ctcttctcac cagctagtgt cacactttac tgggttgtcg
    22861 gtggtttcat gatgattctc caacagttta tcgtcaacta tatcgttcgt ccaaaacttc
    22921 gcaaaaaagt ccgtgaagaa ctagccaaga acccaccaaa agcaagtgct ttctctaaac
    22981 caagtggacg aaaagacgtt acccctgaac aaccaactgc tatcacaagc aagaaaaaac
    23041 acaaaaatcg caacgctgga aaacaacgtt cgagataaga agaaaaaggc ttagctagtt
    23101 tgctaagtct tttttgcaag tcatactctt cgaaaatcaa attcaaacca cgtcagcttc
    23161 gccttgccgt actcaagtac agcctgcggc tagcttccta gtttgctctt tgattttcat
    23221 tgagtatcaa aagcccgatg tttccatcag gcttcttttc tatccatgta cacgtttcat
    23281 ataatcttga taactttcgg tatccatgag ttttttagcg ttcttgacgc gatctgctgt
    23341 tggtggtttg actccttcga gggaatatgg aattccaagt tcacgccact tgaactcacc
    23401 catggtgtga taaggtagaa tttcaaactt atcaacattt ttgagggtct tgacgaactt
    23461 accaagttca atcaagtcat catctctgtc tgtcaatcct ggaactagca cgtggcgaat
    23521 ccagacaggt tttccaatat ctgatagata ctgggcacaa gccaagatat ttttattagt
    23581 ttggctagtg acaatcttgt gctgttcttc gttgatttcc ttgatatcca aaagaaccaa
    23641 gtcagtgaca gccatgagtt tgtcaaactt ctcaaggtaa cgtggtttat tacggaaagg
    23701 aagagcacag gtatccaagg tacagtggat tccttgttcc ttagccttgg tgaagagagc
    23761 aatcaggaaa tcaatctgca agagagcttc tcctccactg actgtaatcc cacccttatt
    23821 tccccagaaa ccacggtagc gcaaggcctc tgtcaagaca tcatctaccg tccgttcacg
    23881 tgacttattg gactccatag cccaagtgtc tgggttgtgg caatactggc aacgcatgtg
    23941 acagccctgc aaaaagacaa taaagcgaat accaggccca tctactgacc caaagctttc
    24001 tgtcgaatgc accattcctg tcacttgtcc ataatcaatt gtttcttcag acatatcgtt
    24061 accttccttg aaaacgtttt atagttttat tatatcacga cttgaaggaa aaacaagatt
    24121 tttgcctatt ttttagaaag caatctgttt ttcaatttca ttttcaatct attatcagat
    24181 tattcttcaa aatcgaagcc atataaggtt gcaaaatagt cctcagggcg ctctgcacgg
    24241 cggatttgac gggctttacc ttcttcggta tagaggattt ccgcagaacg taatttggca
    24301 ttatactggt agcccattga aaatccgtgg gcacctgtat catgaattac cagcaaatca
    24361 ccgatttctg tatgaggcag ttcgcgattg actgcaaatt tatcattgtt ttcacagagt
    24421 gaaccaacca catctaccgc ttcagctggt ccatctggat gggtcacgtt gctaatatga
    24481 tggtaagctc cgtacatagc tggacgcatg aggttgactg ctgaggcatc cacacctaga
    24541 taggtacggt aggtttcctt cttatgagtc actcttgtga ctagagcacc gtgaggtgcc
    24601 agcataaaac gacccaattc ggtgaaaatc ttgacctgac caagacctgc tggcgtaagg
    24661 acctcttcat acaccttacg aactccctca ccaatcaagg cgatatcgtt cggctcctgg
    24721 tctggacgat aattaacacc aataccgcca gaaagattga taaagtctag ccaaatgccc
    24781 aacttttcct tgatttcaac agccagttca aagagctgac gagccaactc tggataatag
    24841 agatgggtca cggtattgga cgctaggaag gagtgaagcc caaaagtctt ggctcctttt
    24901 tccttcaaga tagcaaaggc ttcaaagagc tggtccttgg tcatgccaaa cttagcctcc
    24961 ccaggattat ccataatgtc tgtccccagt tcaaaaacgc ctccaggatt ataacgacaa
    25021 gagatgattt ctggaatgcc tgctactctc tccagatgtt caatatcttc aaaggcatcc
    25081 aagttaatgg tcgcacccaa ttcacgtgca taggcgtatt ccttgtctgg cgtgttgttg
    25141 gaagagaaca taatctcaga acccagaaag tccagtttat ggctcatcaa aagctctaca
    25201 taactagagc agtccacacc acaaccttct tcttggagaa ttttcaaaat agctggagtt
    25261 ggagtagcct taactgcaaa atattcctta aagcccttgt tccacgaaaa agcttggttg
    25321 acggctcttg ccttctcacg aatccccttc tcatcataca agtgaaaggg agtcgggaac
    25381 tcggcaacaa tcgcttctaa ctcttctcta ttgataaatg gtgttttcat aagcttcctt
    25441 ctattctgtt tgcaaagaaa ggctgggaca atcgttccaa cctttagttc tatctcttat
    25501 ttatcttcgt caaagatatt gccaacctca acatcgatgg cttggttctt gacatcaata
    25561 ttggttacct tcaagagtga cttgtagtca aactgctttt cacgttcttc ttgggcattg
    25621 tccgcaaaga ccgctacacc tgccagttct gagtcgaact cgcgcaagag actaatcata
    25681 ccattgaccg ttccgccacc tttcaagaag tcatccacaa tcaagacacg gctgcctgcc
    25741 ttaagactac gttttgaaag gaacattttc tcgatacggt caccacttga acctgaaaca
    25801 tagttgacgc taacagttga accttcggta attttcaggt cacggcgcac aatgacaaaa
    25861 gagacattga ggacattggc aactgcattt gcaagtggca cacccttagt tgctacggtc
    25921 ataaccgcgt caattttttg gtccataaag cttttggcaa taatacgacc aatatttttc
    25981 aagatggctg gtgtgctaag caaatcagac agatagatat aaccacctgg caagatacgg
    26041 tcactttctg acaacttggt acgcaagtct tcaaccattt ccttagcatc ctgactcgaa
    26101 atagacggtg tgaagatgac acctccgcca gccccagtca ctgtctggat atgaccgatt
    26161 tcaatttcct caaaggcgcg tttgataatg acgatatctt ctgagatgga tgatttagca
    26221 gactcatact tttcagcaaa agtattgaga ctagttagtt tataaggatt attaatcaaa
    26281 tagttggaaa tgacaaccat ccgatcactt cttcttaatt tcatttctat ttcctattcc
    26341 atttttattc aaaaaatcaa aaagcaaact agaaagctgg tcgctggtgg ttcaaaacac
    26401 tgttttgaga ttgtcaatag aactgacaaa ccctgtaata tacctgcata tatacatacg
    26461 acaaggcgat actaccctag tttgaagaga ttttcgaaga gtattcattt ttgtctttta
    26521 cttattatac catattcaca taaaaaaacg aacattctta tcctaaaaaa tgctcatttt
    26581 tcttaaatta ttaatctaaa tctggtttat agaaggaacg attatccata gcgaagattt
    26641 tattggtcat ctctccttta tccaccaaag caagagctgt tgacatcatc atcatgcttg
    26701 catccagatt gtcaatcata tggataatct ctgcttccat aatgcgtgga cggactgggc
    26761 ttccatactc aagcaagccg tggtgactga ggatgacatg acgaagcaaa acgacttctt
    26821 ccttggtatc atcgatgccg agttccataa ctgtcttggt aatttcgcta tcaatgagag
    26881 cgatatgtcc aagaagatta cctcgcactg tgtactctgt ctggtctggc cccgtcaact
    26941 cgataacctt agctaagtca tgcagcataa tccccgcata gagtaagctc ttattgagct
    27001 gaggataaac ttcgctaata gcgtctgcca aacgtaccat ggtcgccgta tgataagcca
    27061 agcccgtttc aaaggcatgg tggttggtct tggcagctgg ataggagtag aattccttat
    27121 catacttggt gtagagattt cggacaatcc gttgccagac aggattttca attttgaaaa
    27181 tcatttgcga catgtagtca cgaatttcct tgacatcaac tggtgacttg accttgaaat
    27241 cggctgggtc attgggttca ccagcttgag gcaggcggag agtaatttga ttgacttgag
    27301 gggtattgtt ataaacttct cggcgtcctt tcatgtggac aaccttacct gcggtaaagg
    27361 cctcaatgtt atgaggttgg gcatcccaga gcttcccatc aatctcgcca ctatcatctt
    27421 ggaaggtaaa ggctaggtag tttttcccag ctcgagtttg cctcaggtca gctgatttga
    27481 ttaggtaaaa gccttcaaat agctcatctt ttttcatgtg actaatcttc atattcttcc
    27541 tcattttctt gaaaatggag tagatcaagc gcaggctcgc cttctgacaa ctcaatgtga
    27601 cggagcgtcc gctcgatagc tatggtacga cggtttaata attcatcaat attgccagag
    27661 gcatgttgga gatgtttttg tgccttgacc agaataccac caaacttgcc aaactcggtc
    27721 ttgacactgg caagagtctt gctgatatgg tcggcactct tttggatatt aagggtcttg
    27781 aaaccaactg atagggagtt aagaagggct gatagggtac ttggtcctgc aacaataatc
    27841 tgttcttccc gtctcaaatc atcaaagaag accggattgc gcacaatctc tgaataaaga
    27901 ccttctgtcg gaacaaacaa gattccaaag ttggttgtcc gaggcggtgc tatgtacttg
    27961 ctcttgatat ccttggcaaa gcgcttgacg cttgctagga gtgacttacg acagcgttca
    28021 atctcatcct tgtcacctgt ctcataggct tcttccaagc ggtaataatc tgccagtgga
    28081 aacttagagt caattggcag atagacgtat tcttggtcgc cttgtccggg taacttgatg
    28141 gcatactcca ctcgttcact agagttttca accgttgcgt attctcgttc gtactgggca
    28201 ggtgtcatga tgtcttcaat aatttgcccc agttgcaatt ctcccagaat ccctcgcgtc
    28261 ttggttccag agagaacctt gttaagagct ccgacatcac gggcaactgt ctgcatttct
    28321 ccaaggccac gattgacaga ctccagttgt ttagaaactg tctcaaagga agcctgtaag
    28381 cgtgtctgca aggtcttttc tagtttttcc tcgaccgtct ggcgcatttg ttccaaacgt
    28441 tgctcatttg attcctgcaa ggcttggaga cgttggtctg tcttgtctct agtttggagg
    28501 agattatctg tcatttcttg acggacttgg gtcagacctt ggtgcaattc aatccgcact
    28561 tcttgcaaac ggtcgctgac aaccacttcc aaatcttttt ggtctaactg gctggcttgt
    28621 ctggcttggt caaagcggta atccaactgg tctgacaaat gatctgcctg atcctccaag
    28681 ctcttactta agtgtttctc ctgcctatcc tgcctttgcc aaatcagaaa aagaccagct
    28741 aggttggcaa ttaataatag aataagtaaa ctttccatcc tacctcctgt ccttgctatg
    28801 cagtacgacc acataaccat ctgggcaagt taccgacact tccctatcta tatattcgtt
    28861 agaagcgtac acttttttaa agaaaaaatt ctcctctgtc aactcatact tggctccaag
    28921 aatagtcagc tggctatccc gaactggcat aaaggctaga tagtcgtagt ctgaacgagg
    28981 ttctagctga ctggtccctt ctgaacaata ggcaatcaaa ttttgcccat cctcaatttc
    29041 tatttgacgc atatagggtg ccaacttagg attgctaggt agaaagacat tggccaacat
    29101 atggtcaata cggccaccca aggcaccgaa aatagtgacc tcagcctgag gattttgttc
    29161 aaagatggtt aagagagcca attccagatc tgtatcatcc ttttctggtc gtgcttggac
    29221 aaaatactgg gcacgttttt gaatcacctg tcgctcttct tccgtcacag aatcaaaatc
    29281 tccgactgct agagcaagag gtaagtcttc ttccaagacc cagagcgagc ctcgatccac
    29341 cccaacaaaa gcatcaaaat ctgtccgata atgaccgcgg tttccgcctg caaaaactgc
    29401 aaccctagtc cagttgtttt ctgagagttt gtactcgctc attgacttct cccttaaaga
    29461 cataggaacc tgctacaaaa acagtcgcac cagcttcttt ggcttgggca atagtttggt
    29521 catcaatccc accatccact tcgatttcaa agttcaaacc tttttcctca cgaagagcaa
    29581 ccaactcacg aaccttatcc atggtttctg gcagaaaggc ttgcccacca aaacctggat
    29641 taaccgtcat gactaaaact tggtcaacta gatgaaggac gtgcttgatg gcttcaactg
    29701 gtgtgccagg attgataacg actgaaggct taactccgag tgaacgaatt ttttggaggg
    29761 cgccatgaat atgaggtgtt gcttctacat ggatactgat gatgtctgca cctgcacgcg
    29821 caaaatcttc cagatgatgc tcagggtttg acaccatcaa gtggcaatcg aaaaccatct
    29881 tactatgagg acgaaggctc tcgaccacac ctgcaccaaa actgatttgc ggtacaaaat
    29941 gactgtccat gatatcgata tgggcatatt ctgccccagt tgcttctaga cgtttgattt
    30001 cacgttcaaa gttggcataa tctgctgcca gaattgacgg agcaatcttg tattgagaca
    30061 taggtttctc cttattttgg aatttttttg ctgacttttt tataggtttc tctacgattt
    30121 tcaatttcgc tgaggaattg caggtagttg tcaaaacgga aggtggcaat ggtcccctct
    30181 tctacagctg gcttgacggc acaagacggc tcatgggtat gggtacaagt acgaaactta
    30241 caatctcggc tgacagtagc aatctctggg aaagcctgat tgaggtcttc agcccttgat
    30301 acttcatagt ccagagatga aaagcctggt gtgtctgcga ttttaccccc gttgagattg
    30361 taaaaactaa cagctcgagt ggtatggcga ccgcgaccta gactgtctga aatttctccc
    30421 gtttcaagat tgaggtctgg tacgagttta ttgagaagag ttgacttccc aacacctgtc
    30481 tgccccataa agaccgtaac cttgcctgtt aacaaagaca ggagttcctc tttactggtc
    30541 acaaagtcat agccgatgtc accataggtc tgccggtaaa aatccagttc tcccctatct
    30601 tccaacaaat ccattttgga aatatagaca atgggatgga tgcccttgtg ctccaaaaga
    30661 accaagaaac gatccagcaa attgctgtta aaatcaggtt ccttgacgga catgattact
    30721 acagcttgat cgatattgac aataggcgga cgaaccagac tgtttttccg ttcgtgaatt
    30781 ttgaggatat agccttctga attttcctcg gcagagaaat ctacccagtc cccaacataa
    30841 ggggtatggc cttttttacg gaaattccca cgcgcgcgtg tttgataaac ctggccatca
    30901 ctctccacat agtagaaacc tgccaaggct ttaatgattt gtccctgcat cttagagtcc
    30961 ttgcccttta agtgcgtctg ctagaccggc aaattctgcc aagctgagag cttccccacg
    31021 cacacttggt gacaagcctg cctggtccaa agccttggtc agcttgtcct tgacctcttc
    31081 agtcttacca aagtaacctg tcaagttatt ccacaaggtc ttgcggcgat gggtaaaact
    31141 agccttggaa accttaaaga aaaagttctc atcttctact gctacggctg gctctggacg
    31201 acgcaccatt ttcaagatgg ctgaatccac atttggcgct ggcacaaaga ccgtacgagg
    31261 cacgataaag gcaaccttgg ctgtcatgta atactgcacg gcgatagaca agctaccgta
    31321 agccttggtg ttaggctggg ctgaaatgcg gtccgctact tctttctgca tcatgaccac
    31381 aaactcacaa aaaggaatgc cactctcaat caagtgcatg agaataggcg tcgtgatgta
    31441 gtaaggcaaa ttagccacta ccttgattgg caggtcagga tttttaaaat tctggatatg
    31501 ttgcgccaaa tcaaccttga gaatatcttc gttaactacg gtcacattat caaaatcacg
    31561 cagggtatct gccaaaattg gcaccaaacg gtggtcaatc tcaaaagcca tgacttgggc
    31621 tgcacgctca gccaaaaatt ctgtcaaggc accaatacct ggcccgattt cgatgacatt
    31681 gacctgatca tcaatttcag ccgtatccac aattttttga aggatattgg tatccgtcaa
    31741 aaaattttgc ccaaaggact ttttaaaggt aaaaccgtga cgctccagca ctgccttggt
    31801 cacgctataa tctgcaattc tcatctattc tcttttctat tatctgttac tactattgta
    31861 acacattccc cttgtatttt gggaatgaaa atcataccaa aaaactatct ttcctcatga
    31921 agaaagatag tcttggaaat ctttaaaagt ggccttagca gttctctcga aacatgcgtt
    31981 caaaaaaatc agcttcttga taacttcgat tttcaggtag ggtcaaatcc acatcctcct
    32041 ccacatcatc cactgtcagt tcaaccgtct gaggtaaatt tttcaaacga taatgctttt
    32101 gcctttgccc ttcaatacta gcaaaatcaa gcaaatagcc atgcactcgg atttgaacca
    32161 aaaggagatg attggtcata ttttccatga tgaggatatc ttctaaacca cctctggcac
    32221 taggataatc agataccctg cgaatacgga ttttttcaca catgagcaag tttcctcata
    32281 tcaatgattt catcagcctt attccagact aggggattat gggtcgcaat gatgataatt
    32341 cgattttcat ctttcaaatc caccaagaga ttcataacct cctctgaatt ttcaggatca
    32401 agagctgctg ttggttcatc tgccaaaatc aagggtggat ttttcaaaat agtcttagca
    32461 agggcaactc gttgggcctc tcccccagat aaagtataga ttttttgttc taaatccaaa
    32521 taccctagat taactttttc taaagccccc acttgccttt ccaaacgttc tacttttgag
    32581 attttctgac caacaaaacc caaatccaaa ttttctttga ttgattggtt ttctaagagg
    32641 ccaaaatttt gaaagagata gcccatctga tctcgaaaat actcacgagt gggaatggtt
    32701 tttaaatctt tcccctgata gagaaccctt cctccatcta tcttttctag ctttcccaag
    32761 atattcagca gcgtcgtctt tccgcttcca ctctttccga ttaaggcata aaccttgccc
    32821 ttctccagct tgagattcaa accagagaaa atcgcgcggt cattaaattt cttttccaat
    32881 ccttgaatat caatcatctt attttccttt caatactgcc atgctaccag cttcttcttt
    32941 tttgtcctgc cttactaaaa tcaagagggc gttaagcgta aacaaagcta caactagagc
    33001 gctaatcaaa ccatctcttg tcaaaatact agataggact aggccaagca agagaactcc
    33061 tccttgcgcc agtaaatact tgccatgaag ctcatagatt gtcataccag caagacgttt
    33121 aatcataagc tcccgtctga actgctcaaa atagagaaga ttcatggaat caaataacaa
    33181 gatagccgta gacagggtca aaatcgtccc aataaggaga gagtaaaact ccacccgttt
    33241 gtcatttagt accttggcaa aaaatagctg gctttttacc aagtaagaaa ccttgtgata
    33301 cagaccttgc tcttttagag ctgtgatgct gtctccatat cgatctagtt tcaaggcact
    33361 attagccgta gttccccaca acatccctgc aacaggtctt gttccagtcg cttgcggcgt
    33421 tagaactaca ataatcggat cttttaggta ctggcgtgtt gtcttgtacc catcattata
    33481 gaggaaacgt tcctgtcctg tttctgtaaa agctagcctt acctgtggga agtagtgttt
    33541 ctgactcgtc acttctacac tttcagatga aaagttttgc aggtaatctg taaacaatcc
    33601 ttggtagtag gcagactgct cccgcaagct ctcaggcaag atcagcccaa actctccctc
    33661 agacaagttt tgcatcttgt caataaactc tgaagaaacg gtaatctttt cttctatcag
    33721 atagcgcggc gagacataga taacattccc tgacggtgtg tagtcactga gacgattgcc
    33781 atctaggtcc acttctgctc catctgagaa attgtcaaca ttgctcataa tataaaaaga
    33841 gtctgtattg gctaaccgtt cttcagtaaa tgtctgccac tcacgattat ccttacgcgt
    33901 tccttcttca tcggcaaatg cactagacca accaaaggat agacggtaac ggtctgagga
    33961 ctggctccat ttattgctag ctctctccat ttcctgcatt tcacggtagt ggggtaggag
    34021 agctgtcgca ctcgatccga ccaccaatac agctaagagt tgccccacca tcatcaatgt
    34081 catcatccgt ttgagaggga gtttcccttt caatagatcc accagactat tttcctgtaa
    34141 accaagtaga tagacgacac ttagtaaggt agaaatccct gccagggtca atccataaag
    34201 tagaagagca atgatgacca gttgcaccgt tgccataaac aaggcacctt gataccagag
    34261 aatccccaat cccaaaagac tggataccag cactgagcag ataagctgtc tcacatcttc
    34321 taacactggt ctgagagcaa ctccaaacaa gctctcacca gctattaagc gaatccctgc
    34381 ctgacgaagg gatttgatcc gataaatcag ggtcagactc ataaaggtca gcagaaaaat
    34441 agctaaactc agtagcacca taggggtggc cgtcaatagt aagactatcg aaaatggatc
    34501 ttctccattc gaaacaaagc cttgataacc aagctctttc aaggtggtct gtaagctcac
    34561 tccatccaaa cttcctgaca caatcaagta agaccctaat aaatcactcg tttctgcact
    34621 ctccttggag gaaatggtca gaccttctgg aagctttccc tcaccataag tggcataggt
    34681 aaagcgtgtt tctccagctt catttggctc aacaatccga cgggcgatga gactattatg
    34741 ctctaccgcc aagtgactca aagcctctgt cacctcttcg cggctagcta ctggttgatt
    34801 tatcatcatt cctgtcacat caacagaagg atagttacca aataaaatct cttctcgatg
    34861 gtcaacggaa gttatcacca tataaagcga taccaatacc attgaaatca aaataaataa
    34921 acgtttcatc atttcctcca aaaagttact tggtcactta gtgagaaggt gaccaagtaa
    34981 agctaaacaa tagggctata ccattctaaa cattatagta gaatgcaact ttctctcccc
    35041 aagaagtacg aatccatgca cgagaagtcc ctccagcacc agcatatccc ttgtcagatg
    35101 aaccagtttt tctgctaaca actgttgaac tatggtattt tgaagggtga tagtagtttg
    35161 agtaagcacc accgttacct gaaccataag tccaaacacc accttctggg tattgaacgg
    35221 ctgcagaggc aactgataca cctccaccta atgttgctgc aatgctcgct acaagagcta
    35281 actttttaac tgtttgtttc atattgatac tcctctattt ttgattacaa gattgttgag
    35341 ttacttgctc ctctttccat tatcaacgtt gatactatta tattaccaaa tgatattcta
    35401 aaattatttg tttttctcta aattgtcaaa atagcaccct gaaatgaact tttatagtca
    35461 ttatactcca tctttatcac actatcaagg acatcaatgt ccttaaaa
//
LOCUS       DAVKGU010000013        35004 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKGU010000013 DAVKGU010000000
VERSION     DAVKGU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.013"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(29..838)
                     /locus_tag="VW077_000582"
     CDS             complement(29..838)
                     /locus_tag="VW077_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9454653.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(840..1643)
                     /locus_tag="VW077_000583"
     CDS             complement(840..1643)
                     /locus_tag="VW077_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9454654.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1653..3140)
                     /gene="tacF"
                     /locus_tag="VW077_000584"
     CDS             complement(1653..3140)
                     /gene="tacF"
                     /locus_tag="VW077_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9454655.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFIDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVVPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSSISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3476..4183
                     /locus_tag="VW077_000585"
     CDS             3476..4183
                     /locus_tag="VW077_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9454656.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIEKIIEAIDAYRPL
                     TSEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4188..5210
                     /locus_tag="VW077_000586"
     CDS             4188..5210
                     /locus_tag="VW077_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9454657.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5227..6096
                     /gene="licA"
                     /locus_tag="VW077_000587"
     CDS             5227..6096
                     /gene="licA"
                     /locus_tag="VW077_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9454658.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            6086..6964
                     /locus_tag="VW077_000588"
     CDS             6086..6964
                     /locus_tag="VW077_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9454659.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            6976..7665
                     /locus_tag="VW077_000589"
     CDS             6976..7665
                     /locus_tag="VW077_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9454660.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVCLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7761..8609
                     /gene="dprA"
                     /locus_tag="VW077_000590"
     CDS             7761..8609
                     /gene="dprA"
                     /locus_tag="VW077_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9454661.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9494..10537
                     /locus_tag="VW077_000591"
     CDS             9494..10537
                     /locus_tag="VW077_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9454662.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10705..12792
                     /gene="topA"
                     /locus_tag="VW077_000592"
     CDS             10705..12792
                     /gene="topA"
                     /locus_tag="VW077_000592"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9454663.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMAVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            12898..13257
                     /locus_tag="VW077_000593"
     CDS             12898..13257
                     /locus_tag="VW077_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9454664.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13350..13982
                     /locus_tag="VW077_000594"
     CDS             13350..13982
                     /locus_tag="VW077_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9454665.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            14026..14682
                     /locus_tag="VW077_000595"
     CDS             14026..14682
                     /locus_tag="VW077_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9454666.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            14701..15795
                     /locus_tag="VW077_000596"
     CDS             14701..15795
                     /locus_tag="VW077_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9454667.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGIAVANSLAAVKNGAGRVEETINGIRERAGNAALEEIAVALNIRQDYYQ
                     VETSIVLTETINTSEMVSRFPGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYENI
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAITDGIDAQDRVLVTVENRDTETIFNATGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            15807..16843
                     /gene="leuB"
                     /locus_tag="VW077_000597"
                     /pseudo
     CDS             15807..16843
                     /gene="leuB"
                     /locus_tag="VW077_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            16840..17109
                     /locus_tag="VW077_000598"
     CDS             16840..17109
                     /locus_tag="VW077_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9454668.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17113..17472
                     /locus_tag="VW077_000599"
     CDS             17113..17472
                     /locus_tag="VW077_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9454669.1"
                     /translation="MAGSSREHAAWALANYGFKVVIAGTFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDHVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17634..18204
                     /locus_tag="VW077_000600"
                     /pseudo
     CDS             17634..18204
                     /locus_tag="VW077_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007122522.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
     gene            18298..18363
                     /locus_tag="VW077_000601"
                     /pseudo
     CDS             18298..18363
                     /locus_tag="VW077_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            18526..20457
                     /locus_tag="VW077_000602"
     CDS             18526..20457
                     /locus_tag="VW077_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9454670.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKV
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIGPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            20444..21760
                     /locus_tag="VW077_000603"
     CDS             20444..21760
                     /locus_tag="VW077_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9454671.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKEWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            21935..22921
                     /gene="guaC"
                     /locus_tag="VW077_000604"
     CDS             21935..22921
                     /gene="guaC"
                     /locus_tag="VW077_000604"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9454672.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPTKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            22985..23056
                     /locus_tag="VW077_000605"
     tRNA            22985..23056
                     /locus_tag="VW077_000605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23018..23020,aa:Arg,seq:ccg)
     gene            23239..23937
                     /locus_tag="VW077_000606"
     CDS             23239..23937
                     /locus_tag="VW077_000606"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9454673.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            23928..27467
                     /gene="smc"
                     /locus_tag="VW077_000607"
     CDS             23928..27467
                     /gene="smc"
                     /locus_tag="VW077_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9454674.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            27464..28258
                     /locus_tag="VW077_000608"
     CDS             27464..28258
                     /locus_tag="VW077_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9454675.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYRGQDLYEATMSREFYLTTFEKLKTSPYV
                     DINKLLLTGKKGAYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGIIMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            28258..29076
                     /locus_tag="VW077_000609"
     CDS             28258..29076
                     /locus_tag="VW077_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9454676.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAASDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            29080..30300
                     /gene="ftsY"
                     /locus_tag="VW077_000610"
     CDS             29080..30300
                     /gene="ftsY"
                     /locus_tag="VW077_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9454677.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAVLEESQDSEPAVGVSQEEIEELPEEEKLEHEGTIEENNSEVLEAERPQAEETVQ
                     EKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEELLIMSDVGVQVASNLTEELR
                     YEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQDNLTVMLFVGVNGVGKTTS
                     IGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWGRRVDVPVVTGPEKADPASVVFDG
                     MERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKRVVPEAPHETFLALDASTGQ
                     NALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREELNIPVKLIGFGEKIDDIGEF
                     NSENFMKGLLEGLI"
     gene            complement(30344..31831)
                     /locus_tag="VW077_000611"
     CDS             complement(30344..31831)
                     /locus_tag="VW077_000611"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9454678.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(31980..32720)
                     /locus_tag="VW077_000612"
     CDS             complement(31980..32720)
                     /locus_tag="VW077_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9454679.1"
                     /translation="MAKLKIDVNDLHKYYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(32720..34885)
                     /locus_tag="VW077_000613"
     CDS             complement(32720..34885)
                     /locus_tag="VW077_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9454680.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQSTKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT        10978 a         6107 c         7549 g        10370 t
ORIGIN      
        1 tcttagtagt ttgtataatc cccctcaatc agtttttgcg ataagcttta atgctatgac
       61 tataccactc ttgcatttct tttgatggtg gtgtcatata atcgccatac atctgggtta
      121 aaaattggtc atattttttg ggaacaggca acatacggcc ctcaaactca gttaaaatca
      181 gttctttaaa ggtatcaact gggaagattt ctttcatccc ttccttaccg atcccaactc
      241 ctccttcata ttgaggagtg ttggttacag catttttgac tagttgatca attttcttgt
      301 aaaagtagcg aggattgaca aatcggagag cgtaccagct acataatcta agaaaatctt
      361 ttagtttgct atcaccgtga actgctcgtg attttttgat ataagctagt tgacgaaggg
      421 ccacatactt atagctcttg tcgacaatgc tcaagtctgt aaatcgatca attgggaaga
      481 catcgatgaa aaggctggta tcatgacgct tgtacttaac atggtcttct ataacagtag
      541 aagtgtccaa aatcgatgcg aaattatgga agtaccaaga agatgtatcg taggaaagaa
      601 ccttgtagcg agggtgattt tcttcttcaa taatcttcag taaacgctca taatcctcac
      661 gataaaggga aatatcaata tcatcatccc aaggaatcat acctttgtgg cggatggctc
      721 caagcatggt tccataactg agaaaataag gaatatcatg tttcttacaa gtctcatcaa
      781 tatagtccag cagggctagt tgaatttctt taatttcttt tttttctaaa tattgcatcc
      841 taatcctcca atttataagc gtgaaattca tgactgtaga agcgtttttc ttctggtggt
      901 agggtcatat aatctccata aaattgtgtc aaaatagtat caaatttttc aggtgcagga
      961 aggcttaaat tctcaaaggg taaatcgatt gttttatcaa aggtaccact tgggaagact
     1021 tccttttcct taaattttga agggataaaa gccatatact gcccattttc acgactatat
     1081 ttttgaattt ctttctcgat tttatttgca aaataacgag gagaaactgg tcgaaggagc
     1141 aaccaaaagg ctgttcgtat ccaatctttt aaaaggctat ccttatagac aatattttta
     1201 tgtttactga aagacagcag tttgaagctt tccagtttat aacaagtatc aatgacctta
     1261 ggatcatcaa agcgatccat agggaaaata tcgataaaga taccagactc ataggttttt
     1321 gtatttcgag tatcaataat tttagtcgta ctgtcggtta tcttgataaa gttgttaaag
     1381 tagttcttat cagtttctaa ggataggagt tcatacttgc ttttttcctt ttgaaaaatg
     1441 ttaataaatc gttggtagtc ttctctaggc atggataaat caatatcgtc gtcccaaggg
     1501 ataaagccct catgtcgaac cgccccaatc agagtaccgt agttaataat atagttgata
     1561 ttgtgctttt tacagagagt atcaatataa tccaaaattt ctaattcaat ttgtttggca
     1621 tcttcaacgg ttagttgttt cattttaaac tcctatgatt ttttaaattt attttttaag
     1681 gctaggacat ggtttaaaaa ttcatagaaa atgctatctt ttgtgaagac aagtagacta
     1741 atataagaga tagatgatag caagacaatc aaaccagtat taatcaaaaa tggcaaatta
     1801 atgaccatat ccacaggata cacgaaatta atcaggaaat aaattgctac aaaggaaagt
     1861 gaaaagagag agtatcgaac agtatagcta aagatatgtc ccaagtggat gagttgtttt
     1921 ctatggatga aaatgatata gaaaacaagt agagaagcct ctgataacat agttgtcagt
     1981 aagtagtatt caggaaccac gatatggttg aaaaagagga gactattcaa gcccaaattg
     2041 agtagcccag caaagactgt atagactgtg atacgttttt catagccatt ggtaaagaga
     2101 atttgggaac caagaatggt atctaaggcc aggataatcg tacgaaaagc gaagagagag
     2161 gtcaagatgc cgcctccgat atatttttca ctaccgtaaa gtaggatggc atttggtcct
     2221 aaaaccatga gtccaaaact cagtggaatg ataaagaagt taaagattcg actacctcta
     2281 ttaaccagag aaacataggc ttctttgtct cctttcccca gatagtaact gagacgaggc
     2341 acactcactc caattgcacc tgttacaacc ccagctataa cggtcacaat tcgctgagct
     2401 atggtatagt aactaacgtt gacatcaatc cctgttttaa cgaggaagag gcgatctaaa
     2461 aaagtgaaga gcatattggc attggcaaag actaacatgg ctgtcagagg gagaaagagt
     2521 ggtttaaaat cacttaggtg aatcttaaca agtttgatgt ctcttttaat ccaaaaataa
     2581 ctaatcaggt agttaatcag cgtcgataaa ctcatcacaa gtgtatagac aacaatatcg
     2641 tgttcatttt taacaaataa gaaaatagag accagcatca ggatacggat gaaggcagtt
     2701 ttgtaaaaga gaaaactgta attttccaga gcttcattga cccattcaat tgaaaaaatc
     2761 tgggcaatga gttgaatccc cataacaagg tagacctttt tgacgattgg attatcaata
     2821 aagaagagag gataggctag gatatagaca gcagtggtca aaatcgtaca agcgatgcac
     2881 aaataaaaaa gactagaaaa ggttctgtta agatcttttt tgttatcctt gacattactg
     2941 atagccctta aaccgtagtt atagacacca taagttgcaa agggcaagaa aaatgacaaa
     3001 atagtgtcga ctgagttgaa gtaaccatag tcagttcggt ccaagacacg cgcgacatag
     3061 gttccagtta ggatgggaaa aataatattc aagacacgaa ttcccatgta agatagagca
     3121 tttaatttta tacttttcat tcaatttacc tcgtttttca ttatatcata aagttagcta
     3181 ataagaaatg aagggcagta agtcaagtaa tcactttgaa gtttcaaatc ttaaatttta
     3241 agttttcttt aaggaaagta tattattctg aaggactcta aaatttcgca gccatttatt
     3301 agtaattgct acagaattcc tagtcattac tagaaatgaa ctagtttctt tgaataatag
     3361 aactgcataa ttctcctatt ctagaagggg aggaccagta tttcttttat gataggacta
     3421 gattgtggta taatagagag aataagtttt tttagtaaga caaaggagaa aatagatgat
     3481 ttatgcagga attcttgccg gtggaactgg cacacgcatg ggaatcagta acttgccaaa
     3541 acaattttta gagctaggtg atcgacctat tttgattcat acaattgaaa aatttgtctt
     3601 ggaaccaagt attgaaaaaa ttgtagttgg tgttcatgga gactgggttt ctcatgcaga
     3661 agatcttgta gataaatatc ttcctcttta taaggaacgt atcatcatta caaagggtgg
     3721 tgctgaccgc aatacaagta ttgagaaaat cattgaagcc attgatgctt atcgtccgct
     3781 tacttcagag gatatcgttg ttacccacga ttctgttcgt ccatttatta cacttcgcat
     3841 gattcaggac aatatccaac ttgcccaaaa tcatgacgca gtggatacag tagtagaagc
     3901 ggttgatact atcgttgaaa gtaccaatgg tcaatttatt acagatattc caaatcgtgc
     3961 tcacctttat caaggacaaa cacctcaaac attccgttgc aaggacttca tggaccttta
     4021 tggatctctt tctgatgaag agaaggaaat cttgacagat gcatgtaaaa tctttgtgat
     4081 caaaggaaaa gatgtggcct tggccaaagg tgaatactca aatctgaaga ttacaaccgt
     4141 aacagatttg aagattgcaa aaagtatgat tgagaaagac tagtaaaatg attaatcaaa
     4201 tttatcaact aactaagcct aagtttatca atgtcaaata tcaggaagag gctattgacc
     4261 aagagaatca tatccttatc cgtcccaact acatggctgt ctgtcatgcg gatcagcgtt
     4321 actatcaggg aaaacgtgat cccaagattt tgaataaaaa gcttccaatg gcaatgattc
     4381 acgagtcatg tggaaccgtc atttctgacc cgaccggaac ctacgaggtt ggtcaaaaag
     4441 ttgtcatgat tcccaatcag tctcctatgc agagtgatga agaattctat gaaaactaca
     4501 tgacagggac ccatttcttg tctagtggat ttgatggctt tatgagagag tttgtttctc
     4561 tccctaaaga tcgtgtggtg gcttatgatg ctattgaaga tacggttgca gccattacag
     4621 agtttgtcag tgtgggcatg cacgctatga atcgtctatt gactcttgct catagcaagc
     4681 gggagcggat cgccgttatt ggagatggaa gtttagcttt tgtggttgcc aatattatca
     4741 actatacttt gccagaatca gagattgtgg ttattggtcg tcattgggaa aagttggaac
     4801 tcttctcatt tgccaaagaa tgctatatta cggataatat tcctgaagat ttggcctttg
     4861 accatgcttt tgaatgttgt ggtggtgatg gtactggacc agctattaat gacttgattc
     4921 gctacattcg tcctcaggga acgattctca tgatgggagt tagcgaatat aaagtcaatc
     4981 tcaatactcg cgatgcctta gaaaagggct tgattttggt tgggtcatct cgttctggtc
     5041 gcattgattt taaaaatgct atccaaatga tggaagtcaa gaaatttgcc aatcgtctta
     5101 aaaatatcct ttatctagaa gaacctgtaa gagaaattaa agatattcat cgtgtctttg
     5161 caaccgattt aaacacagcc tttaaaacag tgtttaagtg ggaagtataa gtactggagg
     5221 ttaattgtgg agaaaatcat taaagaaaaa atttcttcct tacttagtca agaagaggaa
     5281 gtcctcagtg ttgaacaact gggtggaatg accaatcaaa actatttggc caaaacaaca
     5341 aataagcaat acattgttaa attctttggt aaagggacag aaaagcttat caatcgacaa
     5401 gatgaaaagt acaatcttga actactaaag gatttaggct tagatgtaaa aaattatctt
     5461 tttgatattg aagctggtat caaagtaaat gagtatatcg aatctgcgat tacgcttgat
     5521 tcaacgtcaa tcaagaccaa gttcgacaaa attgctccaa tattacaaac tattcatacg
     5581 tctgctaagg aattaagagg agaatttgct ccttttgaag aaatcaaaaa atacgaatcc
     5641 ttgattgaag aacaaattcc ttatgccaac tatgaatctg ttagaaatgc agtcttctcc
     5701 ttagagaaaa gactggctga cttaggtgtt gacagaaaat cttgtcatat cgatttggtg
     5761 cctgaaaact ttatcgaatc acctcaagga cgactttatt tgattgactg ggaatattca
     5821 tcaatgaatg atccaatgtg ggatttggct gccctctttt tagagtctga attcacttcc
     5881 caagaggaag aaactttctt atctcactat gagagtgacc aaacaccggt ttctcatgaa
     5941 aagattgcta tttataaaat tttacaagat actatttgga gtctatggac tgtctataag
     6001 gaagagcaag gtgaagattt tggtgactat ggtgtgaatc gttaccaaag agctattaaa
     6061 ggtttggctt cttatggagg ttcagatgaa aagtaaaaac ggagttcctt ttggccttct
     6121 ctcaggtatt ttctggggct tgggtctaac ggttagtgct tatatctttt cgatttttac
     6181 agatttgtca ccctttgtgg tggctgcaac tcatgatttt ttgagcatct ttatcttact
     6241 agcttttctc ttggtaaaag aagggaaagt tcgcctctca attttcttaa atattcgcaa
     6301 tgtcagtgtt atcataggag ccttgctagc aggccctatc ggtatgcagg ccaatcttta
     6361 tgcagttaag tatatcggaa gttctttagc ttcatctgta tcggctattt accctgcgat
     6421 ttcagttcta ttggctttct tctttttgaa gcacaagatt tcgaaaaata ctgtatttgg
     6481 gattgtcttg attattggag ggattattgc ccagacctat aaggttgaac aggttaattc
     6541 tttctacatt gggattcttt gtgctttggt ttgtgctatt gcatggggaa gtgagagtgt
     6601 tcttagctct tttgccatgg aaagtgaatt gagtgaaatc gaagccctct taatccgtca
     6661 agtaacttcg ttcttgtcct atcttgtgat tgtgctcttc tctcatcagt catttgttgc
     6721 agtagccaat ggacaattgc taggtctcat gattgtcttt gtagcctttg atatgatttc
     6781 ctatttggct tattatatcg ctatcaatcg cttgcaacca gccaaggcta caggcttgaa
     6841 cgtgagctat gtagtatgga ctgtcttgtt cgcagctctt ttcttgggaa catcattaga
     6901 tatgctgacc attatgacgt cacttgtcgt cattgctgga gtttatatta ttattaaaga
     6961 ataaaggaga ttcgtgtgaa agccattatc ttagcagcgg gattgggaac tcgcttgcgt
     7021 cctatgactg aaaatacccc taaagccttg gttcaggtta atcaaaaacc tttgattgaa
     7081 taccaaattg agtttctcaa agaaaaagga atcaatgaca tcatcatcat cgttggttat
     7141 cttaaagaac aattcgatta tttaaaagaa aaatacggtg tttgcctcgt tttcaatgat
     7201 aaatacgctg actacaataa cttttactct ctctatcttg taaaagaaga attggccaac
     7261 agctatgtta ttgatgcaga taactatctc tttaaaaata tgttccgcaa tgatttgact
     7321 cgttcgactt attttagtgt ttatcgtgaa gattgtacca acgaatggtt cttggtctat
     7381 ggagatgact acaaggttca agacattatt gttgatagca aggcaggtcg catccttagt
     7441 ggtgtatcct tctgggatgc tccaactgca gaaaagattg tcagctttat cgacaaggct
     7501 tatgcaagtg gtgaatttgt tgatctctat tgggacaata tggttaagga taatatcaaa
     7561 gagctagatg tctatgttga agaattagaa ggcaatagca tttatgagat cgatagtgtc
     7621 caagactatc gtaaattaga agaaattctt aaaaacgaaa attaaagatt ccaacatctg
     7681 acaaaatagt cggatgtttt ttgatttttt acgaactttt acgaatagat agatgagtag
     7741 aaaaagaaat ggagttattt atgaaaatca caaactatga aatctataag ttaaaaaaat
     7801 caggtttgac caatcaacag attttgaaag tgctagaata cggtgaaaat gttgatcagg
     7861 agcttttgtt gggtgatatt gcagatatat caggttgccg taatccagcc gtttttatgg
     7921 aacgttattt tcagatagac gatgcgcatt tgtcgaaaga gtttcaaaaa tttccatctt
     7981 tctctatttt agatgactgt tatccttggg atttgagtga aatatatgat gcgcctgtac
     8041 ttttatttta caagggaaat cttgacctcc tgaaattccc gaaggtagcg gtcgtgggca
     8101 gtcgtgcttg tagcaaacag ggagctaagt cagttgaaaa agtcattcaa ggcttggaaa
     8161 atgaactggt tattgtcagt ggtctggcca agggcattga cacagcagct catatggcag
     8221 ctcttcagaa tggcggaaaa accattgcag tgattggaac aggactggat gtgttttatc
     8281 ctaaagccaa taaacgcttg caagactaca tcggcaatga ccatctggtt ctaagtgaat
     8341 atggacctgg cgaacaacct ctgaaatttc attttcctgc ccgtaatcgc atcattgctg
     8401 gactttgtcg tggtgtgatt gtagcagagg ctaagatgcg ttcaggtagt ctcattacgt
     8461 gtgagcgagc aatggaagaa ggacgcgatg tctttgctat tcctggtagc attttagatg
     8521 gactatcaga cggttgccat catttgattc aagaaggagc aaaattggtc accagtgggc
     8581 aagatgttct tgcggaattt gaattttaaa aatgacctaa gctagaattc taagaaaaaa
     8641 tcaattttaa gagaaaatga acccaacatt tccataataa aacgcatatt agcaagtttt
     8701 taacacttga taatatgcgt tttttctaag tggattagta gagtagagga tttttctcat
     8761 ataatactct tcgaaaatct cttcaaacca cgtcagcttc catctgcaac ctcaaaacag
     8821 tattttgagc tgacttcgtc agtcttatct acaacctcaa agcagtgctt tgagcaacct
     8881 gtggctagct tcctagtttg cgctttgctt ttcattgagt ataagggaaa gtatagtgaa
     8941 ttgaaataag atgtgaacaa ctctatcagg aaagtcaaat taatttatag aaatatttta
     9001 gcagccaagg tgtactgtta tagattcaat tacactataa tttagtgtaa ttgagaaagg
     9061 agaaatgatt gtgattgatg ttggctaggt tatgttcaat gattcctacc gtctcaaatc
     9121 ttgtcagtaa ggaaaaataa attcttcaaa agtagagatt acaaggcttg tttaagaaag
     9181 aattcaaaga ccttgacaaa taaaaataaa atggttatta taagaaatgg tctaaaatag
     9241 atgatgatac ttttcgaaaa tctttttacg tcagctcagc tttgccttgc tgtgttttga
     9301 gcaagctacg gttagcttcc gagtttgatt ttcatttact agaaatgaaa ctgatgagag
     9361 atatcagtag acatttgagt caggatatta tggaaaatga taaaaagagc tcgtgagatt
     9421 ggcatatcag actactaaag tattgagttt gttaggattt tagcgactag ttagctggga
     9481 aaggaagata tttgtgacaa ataataaact gtattcgttg atagaattta gaaataaaat
     9541 atatgaagaa ttagaacttt ccagaagtga tttagcgatt ttactatgtg ccatgcttat
     9601 cgcctctatc ggattaaata tggattcgac tcccgtgatt attggagcca tgttaatctc
     9661 tcctttgatg acacctattc tgggagtggg gctctctcta gctatatttg attttaaatt
     9721 gttaagaaaa tcttttaaaa tattagctat tcaaattctt gccagtctaa tagcttcaac
     9781 actttatttt tatctttctc ccatttcgta tgctagttcg gagattgttg ctagaacctc
     9841 tccgactatt tgggatgttc tcattgcttt tgtaggaggg atagcaggta ttattggtgc
     9901 taggaaaaaa gagaccaata atattgttcc tggtgttgct attgcaaccg ccttgatgcc
     9961 tcctctttgt acagtaggtt atgctattgc ttctgctaat ctaaaattta tcataggctc
    10021 ctcttaccta ttcctcatca attgtagctt tattgtcatt gcgacttata taggtgttag
    10081 gttgatgatg gttaagaaac attattttaa agataatgaa gaagactcta aaatgcgtag
    10141 gattttgctt ctagttgctg ttttgctgat gattccgagt ttcatctctg caacgacttt
    10201 agtgagagaa acgttgaaaa aagagtccct taagaaattt atatcagagc agtttcaggg
    10261 gtataatatt ttgaaaaaaa cctattctaa aaagactcat accctaaagc taaccatttc
    10321 aggaaattat ttgacagaag aagaactcga tatgatttca agtaagagag gtgactatgg
    10381 tttaagtgat gtttctgttc aagtttcaca attgtctgat tcagaacaac ttagtaagga
    10441 agagctggtg gagtatttct tccagtatat caaggataag gaagcaaaag aaaaggagaa
    10501 agccaataaa tttgatacag agtctgagga gcaataattt cttgagaata gctggtttct
    10561 ctcgtgagtc ttctatgtat atcaaaggaa gactgaggtt ttaagtataa aacttttctt
    10621 ctgtttatag tagctaagtc ttgacagggt tcaaaaaatg gtttacactt tataaagttt
    10681 attactttga aaaggtgtga tactgtggct acggcaacaa aaaagaaaaa atcaacagtt
    10741 aaaaaaaatc tagtcatcgt ggagtcgcct gctaaggcca agacgattga aaaatatcta
    10801 ggcagaaact acaaggtatt agccagtgtc gggcatatcc gtgatttgaa gaaatccagt
    10861 atgtccgtcg atattgaaaa taattatgaa ccgcaatata ttaatatccg aggaaaaggc
    10921 cctcttatca atgacttgaa aaaagaagct aaaaaagcta ataaagtttt tctcgcgagt
    10981 gacccggacc gtgaaggaga agcgatttct tggcatttgg cccatattct caacttggat
    11041 gaaaatgatg ccaaccgtgt ggtcttcaat gaaatcacca aggatgcagt caaaaatgct
    11101 tttaaagaac ctcgtaagat cgatatggac ttggtcgatg cccaacaagc tcgtcggatc
    11161 ttggatcgct tggtagggta ttcgatttcg cctattttgt ggaagaaggt caagaagggc
    11221 ttgtcagcag gtcgcgttca gtccattgcc cttaaactca tcattgaccg tgaaaatgaa
    11281 atcaatgcct tccagccaga agaatactgg acagttgatg ctgtctttaa aaagggaacc
    11341 aaacaatttc atgcttcctt ctatggagta gatggtaaaa agatgaaact gaccagcaat
    11401 aacgaagtca aggaagtctt gtctcgtctg acgagtaaag acttttcagt agatcaggtg
    11461 gataagaaag agcgtaagcg caatgctcct ttaccctata ccacttcatc tatgcagatg
    11521 gatgctgcca ataaaatcaa tttccgtact cgaaaaacca tgatggttgc ccaacagctc
    11581 tatgaaggaa ttaatatcgg ttctggtgtt caaggtttga ttacctatat gcgtaccgat
    11641 tcgactcgta tcagtcctgt agcgcaaaat gaggcggcaa gcttcattac ggatcgtttt
    11701 ggtagcaagt attctaagca cggtagcaag gtcaaaaacg catcaggtgc tcaggatgcc
    11761 catgaggcta ttcgtccgtc gagtgtcttt aatacaccag aaagcatcgc taagtatctg
    11821 gacaaggatc agctcaagct atataccctt atctggaatc gttttgtggc tagccagatg
    11881 acagcggccg tttttgatac catggctgtt aaattgtctc aaaatggagt tcaatttgct
    11941 gccaatggta gtcaggttaa gtttgatggt tatcttgcca tttataatga ttctgacaag
    12001 aataaaatgc taccagatat ggctgttgga gatgtggtca aacaggttaa tagcaaacca
    12061 gagcaacatt tcacccaacc gcctgcccgt tattctgaag caacactgat taaaacctta
    12121 gaggaaaatg gggttggacg tccatcaacc tatgcgccaa ccattgaaac cattcagaaa
    12181 cgttattatg ttcgcctggc agccaaacgt tttgaaccga cagagttggg agaaattgtc
    12241 aataagctca tcgttgaata tttcccagat atcgtaaacg tgaccttcac agctgaaatg
    12301 gaaggtaaac tggatgatgt cgaagttgga aaagagcagt ggcgacgggt cattgatgcc
    12361 ttttacaaac cattctctaa agaagttgcc aaggctgaag aagaaatgga aaaaatccag
    12421 attaaggatg aaccagctgg atttgactgt gaagtgtgtg gcagtccaat ggtcattaaa
    12481 cttggtcgtt ttggtaaatt ctacgcttgt agcaatttcc cagattgccg tcatacccaa
    12541 gcaatcgtga aagagattgg tgttgagtgt ccaagctgtc atcagggaca aattattgag
    12601 cgaaaaacca agcgtaatcg cctcttctat ggttgcaatc gctatccaga atgtgaattt
    12661 acctcttggg acaagcctgt tggtcgtgac tgtccaaaat gtggcaactt cctcatggag
    12721 aaaaaagtcc gtggtggtgg caagcaggtt gtttgtagca aaggcgacta cgaggaagaa
    12781 aagattaaat aagaggaggg gcccgaaagt aaatttcagg ctccttttgg ttagaacttg
    12841 acaaaataca tcattttatg cgaaactaga agaagattat tttaactagg agaagttatg
    12901 cgtcttatct atctgataat tggtttttta tcactggcct tggctattgt tggggttgtt
    12961 ttacccttgt tgccgacaac gcctttcctt ttgttgtcta ttgcttgttt ctccagaagt
    13021 tccaagcgtt tcgaagactg gctttatcat actaagctct atcaaacata tgtagctgat
    13081 tttcgcgaga ctaagtctat tacgcgtgaa cgaaagaaaa aaatcatcgt ctctatctac
    13141 gtcttgatgg gaatttctat ttattttgca cctcttttac cagtcaaaat cggtctgggt
    13201 gctttgacca tctttatcac ttattatctt ttcaaggtca ttccagacaa agaatagtta
    13261 aaacagtagt tatttgcctt gataaaattg aaagcatatt cataataata tgatataata
    13321 aatttgaagt aatattcaag gagaatcaaa tgatttacga attttgtgct gaaaatgtga
    13381 ctttacttga aaaagcgatg caggctggag ctcgtcggat tgaactctgt gataatctag
    13441 cagttggtgg gacaacaccc agctatggag tgactaaggc agcggttgaa ctggcagcta
    13501 actacgatac aaccatcatg accatgattc ggccacgtgg tggtgacttt gtctataatg
    13561 acctagaaat tgctatcatg ctagaagaca ttcgtttgac tgctcaggct ggaagtcaag
    13621 gggttgtatt tggaacttta actgctgata aaaagttgga taagcctaat ctggaaaagt
    13681 taattgctgc atcaaaagga atggaaattg tctttcacat ggcctttgat gaactaagtg
    13741 atgaagatca accggaagct attgactggc tcagtcaagc cggtgtcact cgtatcctaa
    13801 ctcgtgctgg tgtgtctggc gactccttag aaaaacgttt tgttcactat cacagaattt
    13861 tggagtacgc taaaggtaaa attgaaattc taccaggtgg ggggattgac cttgaaaacc
    13921 gtcaaacctt tatcgaccag gtgggggtaa cacaattgca tggtactaag gttgtttttt
    13981 aaaaaataga aaggaactgc tagctttggg tagcagtttt cacttatgtt tgaaattttt
    14041 aaatcctatc aatttaatca agaaaaggct catgattatg gttttataga aaatagcgaa
    14101 gtctggacat atagttgcca gattttgcaa ggtgactttg tcatgactgt gtccatcact
    14161 gctgataatg tgaactttca agtctttgac caagagactg gtgacctcta tcctcacgtt
    14221 catatggaaa gcatgagggg aagttttgtc ggaaatgtcc gtgaggcttg tctggagatt
    14281 ctttaccaga ttcggaaggc ttgttttgat gtgcaagatt ttatctgtca tcagactaag
    14341 cgtatcatga ctcaagttca ggaaaagtat ggaaaccagt tggagtatct gtgggaaaaa
    14401 tcgcctgata cagctgtatt gcgccatgaa ggcaatcaaa agtggtatgc cgtcttgatg
    14461 aaaatctctt ggaataagct ggaaaagggc agagaaggac aagtggaagc agtcaacctc
    14521 aagcatgacc aagtagctaa tttgctttca caaaagggga tttatccagc cttccatatg
    14581 agcaagcgct actggattag tgtgtccctt gatgatactt tatcagatga agaagtactg
    14641 gaattgatag aaaaaagttg gaacttaacc tctaaaaaat gaaatatttt aataattttc
    14701 atgaactttc aattagctaa atattcttta ctgaagaaat tttcagaaaa tataggattt
    14761 accacaccag aggaatatgg tgccatcttc aaatacctga ttgagaatgt caagacggat
    14821 cgtcagatca tctattcgcc tcactgtcat gatgacctcg gaatagcagt ggcaaatagc
    14881 cttgctgctg tcaagaatgg tgcaggacgt gttgaagaga ctatcaatgg tattagggag
    14941 cgagctggaa atgctgcttt ggaagaaatt gcagtggctc tcaatattcg ccaagattac
    15001 taccaagtag aaaccagtat tgtcctaact gagaccatca atacgtcaga aatggtttct
    15061 cgcttccctg gtattccagt tcctaaaaac aaagccgtcg ttggtggcaa taccttctcc
    15121 cacgaatctg gtattcacca agatggagtc cttaaaaatc ctctcactta tgagaacatc
    15181 acacctgaat tggttggtgt taagattccg cttggaaaat tatctggtcg ccatgctttt
    15241 gttgagaaac tgagagaatt ggccctagat tttacagaag aggatatcaa accactcttt
    15301 gctaagttca aggcactggc cgataagaag caagaaatca cagatgcaga tattcgagct
    15361 ttggtagctg gaaccatggt tgaaaatcca gaaggcttcc actttgatga tttacaactt
    15421 caaactcatg cagataatga cattgaagcg ctcgttagcc tagccaatat ggatggtgag
    15481 aaagtcgaat ttaatgcgac agggcaaggt tccgttgaag caatctttaa cgctatcgat
    15541 aagttcttta atcaatctgt tcgtttggtg tcctacacta tcgatgcgat aacagatgga
    15601 atcgatgccc aggatcgggt tttggtcact gttgaaaaca gagatacaga aaccatcttt
    15661 aatgcaacag ggcttgattt tgatgtgttg aaggcttctg ctattgtcta tataaacgct
    15721 aatacctttg ttcaaaaaga gaatgcaggt gagatgggac gcagtgtttc ttaccacgat
    15781 atgcctagtg tgtaaaggag aaggctatgg caaagaaaat agtagctcta gtaggagacg
    15841 gaattggccc agaaatcatg gaggctggtt tagaagttct ggaggctcta gctgaaaaac
    15901 aggttttgac tatgagattg acagacgacc gttcggaggt gcagatattg atgcagcagg
    15961 acctccctta cctgatgaaa cccttaaggc aagtagggaa gcagatgcta tcctactagc
    16021 agctatcggt agtcctcagt atgatggagc agcggttcgc cctgaacaag gcctgatggc
    16081 tctccgtaag gaactcaatc tttacgctaa tattcgtcct gtaaaaatct ttgacagtct
    16141 caagtatttg tcaccactta aaccggaacg aatttctggt gtagactttg tcgtggtgcg
    16201 tgaattgaca ggcgagattt actttggaga tcatattctt gaagagcgca aagcgcgtga
    16261 tatcaacgac tatagctatg aggaagtgga gcggattatt cgcaaagcct ttgaaattgc
    16321 aagaaatcgc agaaaaatcg ttactagtat cgataagcaa aatgttctag cgacctcaaa
    16381 actctggcgg aaagtagctg aggaagtcgc acaggatttc ccagatgtaa ccttggaaca
    16441 ccagctggta gactcagctg ctatgcttat gattaccaat cctgctaagt ttgatgttat
    16501 tgtaacggag aatctttttg gagatatttt atctgatgaa tcaagcgtct tatctggtac
    16561 acttggggtt atgccatcag ccagtcattc tgaaaatgga ccaagtctct atgaacctat
    16621 tcacggttca gcacctgata ttgcaggtca aggaattgcc aatcctattt ccatgatttt
    16681 atcagtttcc atgatgttga gagatagttt cggacgttat gaggatgcag agcgtatcaa
    16741 acgtgctgtt gagacaagtc tggcggcagg aattttaacg agagatatag gaggtcaggc
    16801 ttcaacaaag gaaatgacgg aagctattat tgcaaggtta tgaagttaga cgaaaaaatt
    16861 actctagtcc ttttgatttg gaatgtcatc attttcttga tttatggcat tgacaaattc
    16921 aaggcaagaa gaagagcttg gcgcatccaa gagaaaatct tactcatttt agcccttact
    16981 tgtggtggtt ttggggcctg gttagctgga atcacttttc accacaagac tcgaaaatgg
    17041 tactttaaaa cagtttggtt tcttgggatg gtgaccacac tagtagcctt atattttatt
    17101 tggaggtaat ggatggcagg gtcttcgagg gaacacgctg cttgggctct agcgaactat
    17161 ggttttaagg tcgtgattgc aggaactttc ggtgacattc attacaataa tgaactcaat
    17221 aatggcctgt tgccaatcgt tcagcctaga gaggttagag agaaattagc ccagctaaaa
    17281 ccaaccgacc atgtaactgt ggacttggaa caacaaaaaa tcatctcacc agttgaagaa
    17341 ttcaccttcg agatagatag cgagtggaaa cataaactcc taaatagttt ggatgatatc
    17401 ggtattacct tgcagtatga agagttgatt gctgcttatg aaaaacaacg accagcctac
    17461 tggcaggatt agaaaaaata gaaaaggaaa tatagtaaac tgaaataaga tgtaaacaaa
    17521 tgaattggag cttaacatcc atttccagca attttttaga aactacagtg gactattctg
    17581 gattcaacac attataaaat tatgacaaaa cacattcaca agaaggctac gacattttaa
    17641 aaggtgaggg cggatgtatc gcttgcccta ctaaagttgg ttacattatc atgaccagca
    17701 acaaggcaga acttgagcgt aagttcgcag ccaaagaacg taagcgtaac aaaccaggtg
    17761 ttgttctctg cggtagcatg gatgaacttc gcgctttagc gcaactcaac ccagaaattg
    17821 aagcattcta ctaaaaacat tgggatgaag atattcttct tggttgtatc cttccttgga
    17881 aaccagaagc ctctgaaaaa ctcaaagcat acggggatgg ccgtgaagaa cttatgactg
    17941 atgtacgtgg tactagctgt tttgttatca agtttggaaa agcaggtgaa caattggctg
    18001 ccaagctttg ggaagaaggt aaaatggtct acgcctcatc tgcttcaatg acaaaacgat
    18061 tgaaactcgc tatgagagca aggtgtaatg gtgtctatgg tcgataagga tggcaaactc
    18121 atcccagaac aaggaggagc acgttcaacc tcaccagcac cagttgtgat ccgtaaaggg
    18181 cttgacattg atatagtaaa atgaagtaag agcaggacaa attaatcagg acagtcaaat
    18241 cgatttctaa caatgtttta gaagtagagg tgtactattc tagtttcaat ccactataaa
    18301 atcatgatgc acctgtcaga tacttttaac tcatgggact accgtcaggt tgagtattat
    18361 taggatagag aagaagtcta gtgttaagga cattacagct cataatacta ggctttttta
    18421 tagaaattct tttctttttc tataggatat ggtattctat gtagaaaata tatgttaata
    18481 agtaatgcca atatttaaac atcattagta aaaggagtta gattgatgaa taaaagaaaa
    18541 gttagtttag aagattttta taaatggtat agtctaaata aagaagagtt attaaataag
    18601 gcaactgttg gtgaaaagtt taatgataaa ttaaaagaag agtttctcca ggaatggcct
    18661 ttggatagga ttttaacaat gtcaatcgat gaatatgtaa taggaaaggg acagcaaaat
    18721 aagtctttat gctacgctct tgagaaggga aaatacaaaa atctatttct tggaatttct
    18781 ggtggctcag cttcaaaatt tggtatttat tggaataaaa aaacaaacaa atataaagat
    18841 caagctaata atgagatttc agagttggat cagcgatttt caaaattaaa atcagatttg
    18901 tatgaaatta tcaaagaagg tattcgtttt aactttgaaa atcctatttt tgatatgaaa
    18961 agatcaacaa atgaatttat tggtcgttct gctatggtga caaaattact ttgtatctat
    19021 tctgagggag atcctttctt tggtgtaaat attaatagtc agaaagaatt ttggaaccac
    19081 tttgtttctc agacaaatca aggtggacct tatctgcaaa atcataaaat aattgaactg
    19141 gtgtccaaaa cttatcctga gttggagcca tcgaaagtag gaactatgct ttttgagtat
    19201 tctaagcttt ttatggaaaa taaggaagac aatagtacaa tggattcatc aaacaatttt
    19261 cgtcatcaat taactcaatc tctattaaag tctccaaacc ttatccttcg tggtgctcct
    19321 ggcacaggaa agacttatct tgctaaagaa attgccaaag aattaacgga tggcaacgaa
    19381 gaccaaatcg gatttgtaca atttcaccca tcatatgatt atacggattt tgtagaaggt
    19441 ttaagaccag tatcaaatgg ggatggagct attgagttta ggctacagga cggtattttt
    19501 aaagattttt gtcagaaagc aaaagaaacc caattgattg gaggacaaga taattttgat
    19561 gaggcttggg attcttactt agaatatata aatgttgctg aagaaaaaga atatataaca
    19621 aaaacatctt acttatctgt taatagtaga caaaatttgt cagtaaatta tgatagtggt
    19681 gttccaggat ggtcactacc tagcaaatat gtttacgagt tgtataaaga taaaaattat
    19741 aataagcaag aatactacaa aagtggtgga aaaactgtcc tagaaacatt gagaaagaga
    19801 tttggtttga aagactatgt ttccccaaca gaaattgata ctgataagaa attcgtcttc
    19861 atcatcgatg aaatcaatcg tggggagatt tctaagattt ttggcgaact ctttttctct
    19921 atcggccctg gctatcgtgg tgaaaaagga agtgtttcta cccaatatgc aaatctacac
    19981 gaaactgatg aaaagttcta tatccccgaa aatgtttaca tcatcggaac tatgaatgat
    20041 attgatcgtt cagtggatac ctttgatttt gctatgcgtc gtcgttttcg ttttgttgaa
    20101 gttactgccg agggtcaagt tggcatgttg gataaagagc tgaatatcca tgcagaagaa
    20161 gcaaaaattc gtctaagaaa cttgaacgct gctatcgaaa atgttcagga attaaacagt
    20221 cattatcata ttggaccaag ttattttctt aagttgaagg atgtagattt tgactatgaa
    20281 ttactctggt ctgattatat taagcctctc ctagaagact acttgcgagg ttcttatgat
    20341 gaggttgaaa ctttggaaac tttgaaaaaa gcatttgaac tgacaaataa tgagcaaaaa
    20401 gatcaggcag tagctgatga caatgaaggt gatgaaaacg atgatgcgga ttactgataa
    20461 tcaacacaag attattaaag aaaaatttgt tgaagaatat cctaaactaa gcaatcttct
    20521 tttagacaga accttggaaa gtttatccca agatgaacgt attttcattt ttccaaatga
    20581 tttgactcat actcctgatt tggataagga ccaaaagatt tttgaaacag tcaatcagaa
    20641 aatcaagaca gggaacgtga ttggttttct tggatatggt caggaaagat taacgatttc
    20701 ctcacgattt tctgatgaga gtaatgacca ttttttgcat tatctcttaa acaaggttct
    20761 tcatatcaat ctcactagtt tagatgttgc tttgtctcgt gaagagaggc tttatcaact
    20821 tttggtgtat ctctttccca agtatctaca agctgctatt cgaaaaggtc tttataagga
    20881 atatcatcga ttttctcata acgacagtaa tgttaaggga gtgattgatg taagaagcca
    20941 tctcaagaaa aatcttcctt tcacgggaaa tattgcctac gcaacgagag agttcaccta
    21001 tgataatccc ctcatgcagt tggtccgtca cactattgaa tacattaaga atcagaaaag
    21061 cattggtcaa ggggtactag ataatctctc aactagtcgt gaaaacgtat ctgaaatcgt
    21121 gcgtgtaacg ccctcttata aactagctga tcgtgctaag attattcggg gaaatcaatc
    21181 taaacctata cgtcatgcat actttcacga gtacagaaac ttacaagaac tttgtctgat
    21241 gatcctaaac caagaaaagc acggtttagg gtatcaagat caaaaaatct atggtattct
    21301 ctttgatgtt gcctggcttt gggaagagta tgtttacacc ttgttgccaa aaggttttgt
    21361 acatcccaga aataaggata agacggatgg aatttcagta ttttctgttg ggaaacgaaa
    21421 agtatatcca gatttttatg acagagaacg aaagattgtt ctagatgcaa aatataaaaa
    21481 actggaattg actgaaaaag gaatcaaccg tgaggactta ttccagctga tttcctattc
    21541 ttatatttta aaagctgaga aggctggact gatttttcct agtatggagc agtcagtaaa
    21601 tagtgaaata ggaaaagtag ctggctatgg agctcaattg aaggagtggt ctattcgaat
    21661 ccctcagaat gcctcattct atagtacatt ttgtaaaatg atggaaaatt cagaagaaaa
    21721 ttttaaagcg attattgatg aagaagtggg gagaaaataa tttcccctct ttcttttatt
    21781 tttaacacaa gtaaaacccg aacgatatat tttaattcat aaaataattt gacaaaaact
    21841 gtactttggt ttataataaa ttaaggaaac gtcggaaaag gcgtagtgat gcaagagcat
    21901 aggtaggtca ttacaaaaga aacgagacat cgatatgtta aatgaatttc caatttttga
    21961 ttacgaagat attcaattga ttccaaataa atgtgtcatt aaaagccgtg cagaagcgga
    22021 tacaagtgtc actttaggaa atcacacctt taaactacct gttgtgccag cgaatatgca
    22081 gacgattttg gatgaaaatg tagcagagca actggctaaa ggtggttact tctacattat
    22141 gcaccgtttt gatgaggcag gacgcattcc ttttattaaa cgaatgcaca atcaagggct
    22201 cattgcttcc atatctgtcg gtgttaagga ctatgagtat gatttcgtta gacagctcaa
    22261 gactgatgct cctgagtata tcactattga tattgctcat ggtcatgcgg atagcgtgat
    22321 ttctatgatt caacacatca agaaagaatt gccagatacc tttgtcattg ctggaaatgt
    22381 gggaacacca gaagctgtgc gtgaattgga aaatgctggt gcggatgcta ctaaggtcgg
    22441 aatcggtcct ggtaaggttt gtatcaccaa ggttaagact ggttttggta caggtggttg
    22501 gcagttggct gctctacgct ggtgtgccaa ggctgcgcgt aaaccgatta tcgctgatgg
    22561 aggaattcgt actcacggtg atattgccaa gtctatccgc ttcggtgcta gcatgatcat
    22621 gattggttcc ctctttgcag gacatattga aagtccaggg aaaacgattg aagtcgatgg
    22681 tgaacagttc aaagaatatt atggttcagc ctcacaatat caaaaaggtg cttacaaaaa
    22741 tgtggaaggc aaacgtatct tgcttcctac taaaggtcat ctgcaagaca ctttaactga
    22801 gatggaacaa gaccttcaaa gtgctatttc ttatgcaggt ggacgtcagg ttgctgacct
    22861 taaacacgtt gattatgtga tcgtgaaaaa ctccatctgg aatggggatg cttcccacta
    22921 agacggggtc tttgactaaa aaacttgtta ttagagcaaa tttctgttat aataaaacaa
    22981 gtttccaccc ttagtgtaat ggatatcacg taagattccg gttcttgaga tgggggttcg
    23041 attccctcag ggtggatgta aacattctaa aaagccttta atagggcttt tttctttaca
    23101 ctactctaaa tttgccacta aaactgagaa tctctgtcta aagaattccc aaatgctttc
    23161 cagtcccgtt ttagagtgac tcagggggct ttttttgata taataaaaag gactgttatc
    23221 agttagaaag aggttggtat gaaagaatta caaactgtac taaagaacca ttttgcaatc
    23281 gaatttacag acaaaaagtt actggaaact gcctttactc atacgagtta tgccaatgag
    23341 caccgcctct taaaaatttc acataatgaa cgcttggaat ttttaggaga cgctgttcta
    23401 cagttattga tttcagaata tctgtataaa aaatatccta aaaagcctga aggtgaccta
    23461 tcaaaactcc gtgctatgat tgtccgtgag gagagtttag ctggttttgc gcgtgattgc
    23521 cagtttgacc agtttatcaa gttgggtaaa ggggaagaaa aatctggtgg tcgcaatcgt
    23581 gacaccattc ttggtgatgc ctttgaagcc tttcttggtg cccttctttt ggataaggat
    23641 gtggccaagg tcaaggaatt tatctatcaa gtcatgattc ctaaggttga agcaggcgaa
    23701 tttgagatga ttacagacta taaaacccat ctccaagagt tacttcaggt caatggtgat
    23761 gtggctattc gttatcaggt gatttctgaa acagggcctg ctcacgataa ggtctttgat
    23821 gtagaagttc ttgttgaagg taagagcatc ggtcaaggcc aaggtcgttc taagaaatta
    23881 gcagagcagg aagctgccaa aaatgccgtt gagaaggggc tggattcatg tatttaaagg
    23941 aaatcgaaat tcaggggttc aagtcttttg ctgataagac caaggtcgtt tttgaccaag
    24001 gtgtgacggc agttgttgga cccaatggat ctggaaagtc caatattaca gaaagtctgc
    24061 gttgggcttt gggggagtct agtgtcaaga gtctccgtgg gggcaagatg ccggatgtca
    24121 tctttgctgg aacagaaagt cgcaaaccgc tcaattatgc ttctgtagtt gtgactctgg
    24181 ataatcatga cggatttatc aaggatgcag gtcaagaaat cagggtggaa cgccatatct
    24241 atcgtagtgg agatagcgaa tacaagattg acggcaagaa agtccgtctg cgtgatattc
    24301 atgacctttt cttggataca ggtttgggac gagattcctt ctctattatt tcccaaggga
    24361 aggttgagga gatttttaat tccaagtctg aggaacgacg agctattttt gaagaagctg
    24421 ctggagtttt aaaatacaag actcgcagaa aagaaaccga gagtaaactg cagcaaactc
    24481 aggataatct ggaccgctta gaggacatta tctacgagtt ggataatcaa atcaagcctc
    24541 ttgagaagca agctgagaat gcccgtaaat ttctagactt agaaggtcaa cgcaaggcta
    24601 tttacttgga tgtattggtt gctcaaatca aggaaaataa ggcagaacta gagtcgacag
    24661 aagaagagtt ggctcaggtt caagaactct tgatgagtta ttaccaaaag cgtgaaaaat
    24721 tagaagaaga aaatcaaact cttaaaaagc aacgccaaga tttacaggct gaaatggcca
    24781 aagaccaagg cagtttgatg gacttgacta gtctgattag tgatttagaa agaaaattag
    24841 ccctatcgaa actggagtct gagcaagtgg ccttgaatca acaggaggca caagctcgtt
    24901 tggctgcttt ggaggataag agaaattcac tcagcaaaga aaagtctgat aaagaaagct
    24961 ctttagctct gttagaggga aatctagtcc aaaataatca aaaactcaat cgtttagaag
    25021 ctgaattgct ggctttctca gacgatcctg atcagatgat tgagctctta cgtgaacgct
    25081 ttgtagctct tttacaagaa gaagcggatg tctcaaacca gttgacccgt attgagaatg
    25141 agttggaaaa tagtcgtcag ctttctcaaa aacaagcaga tcaactagaa aagctgaaag
    25201 agcaattagc tatagctaaa gagaaggcta gtcagcaaaa ggaagagctt gaaactgcca
    25261 aggagcaggt tcagaaatta ttggctgact accaagctat tgccaaggag caagaggagc
    25321 agaaaacttc ctatcaagct caacaaagcc aactctttga ccgtctggat aatctcaaaa
    25381 acaagcaggc cagagctcaa agtttggaaa atatcctgag aaatcatagt aacttttatg
    25441 caggtgttaa gagtgttctc caagaaaaag atcgcctagg tgggattatt ggtgcagtca
    25501 gtgagcatct gacctttgat atgcattatc aaactgccct agaaattgcg cttggagcga
    25561 gtagccaaca tatcatcgta gaagatgaag agtcggcaac caaggctatt gacttcctta
    25621 aacggaacag agccggtcgt gcaacctttc ttcctttgac cactatcaag gcgcgtacga
    25681 tttctagtca gaaccaagat gctatcactg taagcccagg tttccttggg atggcagatg
    25741 agttggtgac ttttgatact agactggaag ccattttcaa gaacttgcta gctacgacgg
    25801 ctatctttga tgccgtagaa catgcgcgtg cagcagctcg ccaagttcgt tatcaggttc
    25861 gtatggtgac attggatggg acagaattac gcacgggtgg ttcctatgcg ggtggtgcca
    25921 atcgccaaaa taacagtatt ttcatcaagc cagaactgga gcaattacaa aaagaaattg
    25981 ctgcagatga agcaagcttg cgttcagaag aagcggcttt gaagacctta caagaccaga
    26041 tggctacatt gacagaaaga ttagaagtca tcaaatctca aggagaacag gcacgtattc
    26101 aggagcaagg cttgtccctc gcttatcagc aaactagtca gcaagttgaa gaactggaaa
    26161 ctctttggaa actccaagaa gaggaaatag atcgtctttc cgagggagat tggcaagcgg
    26221 ataaggaaaa atgccaagat cgtcttgctg caatcgccag tgacaagcaa aatctggaag
    26281 ctgagattga agagattaag tctaacaaaa atgccatcca agaacgctat caaaacttgc
    26341 aggaagagct agcgcaagct cgtttgctta agacagaact gcaagggcaa aaacgttatg
    26401 aagttgctga tattgaacgc ttaggcaagg aattggacaa tcttgatttt gaacaagagg
    26461 aaatccagcg ccttcttcaa gaaaaggttg ataatcttga gaaggttgat acagaattgc
    26521 tcagtcaaca ggcggaagaa gccaaaactc agaaaacaaa tctccaacaa ggtttgattc
    26581 gcaagcagtt tgagttggat gatattgaag gtcaactgga tgatattgct agtcatttgg
    26641 atcaggctcg ccaacagaat gaagagtgga ttcgcaagca aacacgtgct gaagccaaga
    26701 aagaaaaggt tagcgagcgc ttgcgctatt tacaaagtca attaacagac caatatcaga
    26761 ttagctatac tgaagctcta gaaaaggcgc atgaattgga aaacctcaat ctggcagagc
    26821 aagaggtgca ggatttagag aaagctattc gctcactggg gcctgtcaat atagaagcta
    26881 ttgaccagta cgaagaagtt cacaatcgtc tggatttcct aaatagccaa cgagatgata
    26941 ttttgtcagc gaaaaatctg ctccttgaga ccattacaga gatgaatgat gaggtcaaag
    27001 atcgctttaa atcaaccttt gaggccattc gtgaatcctt taaagtgacc ttcaaacaga
    27061 tgtttggagg aggtcaggca gacttaatct tgactgaggg agacttgttg acagctgggg
    27121 ttgagatttc tgttcaacct ccaggtaaga aaatccagtc gcttaacctc atgagtggtg
    27181 gtgaaaaagc cttatcagct cttgctttgc ttttttctat cattcgagtt aagactatcc
    27241 catttgtcat cttggatgag gtagaggccg cgctggacga agccaatgtt aaacgttttg
    27301 gggattacct caatcgattt gacaaggaca gccagtttat cgtcgtaacc caccgtaagg
    27361 gaaccatggc agcggctgat tctatctatg gagtgaccat gcaagaatcg ggggtctcaa
    27421 aaattgtttc ggttaagtta aaagatttag aaagtattga aggatgacaa ttaaactagt
    27481 agcaacggat atggacggaa ccttcctaga tgggaatgga cgctttgata tggaccgcct
    27541 caagtctctc ttagcttcct acaaggaaaa agggatttac tttgcggtgg cttcggggcg
    27601 cggatttctg tctctagaaa aattatttgc tgatgttcgt gatgacatta tttttatcgc
    27661 agaaaatggc agtttggtag agtatcgagg tcaagacttg tatgaagcga ccatgtctcg
    27721 tgagttttat ttgacgactt ttgaaaagct gaaaacttca ccttatgtag atatcaataa
    27781 attgctcttg acggggaaaa agggagctta tgttctagat acggttgatg agacctattt
    27841 gaaagtgagt cagcactata atgaaaatat ccaaaaagta gcgagtctgg aagatattac
    27901 agatgacatt ttcaaattta cgaccaactt cacagaagaa acgcttgaag ctggggaggc
    27961 ttgggtcaat gaaaatgtcc ctggtgttaa ggccatgaca actggctttg aatccattga
    28021 tattgttctg gactatgtcg ataagggtgt ggccattgtt gaattggcta aaaaacttgg
    28081 catcataatg gatcaggtta tggcttttgg agacaatcta aatgatttgc atatgatgca
    28141 ggttgtggga catcctgtag ctcctgaaaa tgcacgacct gaaattttag aattagcaaa
    28201 gactgtgatt ggtcaccata aggaccagtc ggttatagct tatatggagg gcttataatg
    28261 gcagatataa aattaatcgc attggacttg gacggtacct tgctgactac tgataaaagg
    28321 ctgacggatc gcaccaaggc aactttgaaa gctgcgagtg atcgtggtat caaggtcgta
    28381 ttgacaactg gtcgtccctt aaaagccatg gacttctttc tccatgagtt agggactgac
    28441 ggccatgaag atgagtatac cattactttt aatggtggat tagttcagaa aaatacagga
    28501 gaaatccttg ataaaacagt cttttcatat gatgatgtgg cacgtttgta tgaggaaaca
    28561 gagaaattat cactgcctct tgatgccatc tcagaaggaa cagtttatca aatccaatcg
    28621 gatcaagaaa gtctttatgc caaattcaat ccagctttga cttttgttcc agttgacttt
    28681 gaagacctgt ctagtcaaat gacctacaac aaatgcgtga ctgcctttgc tcaagaaccc
    28741 ttggatgcag ccattcagaa gatttctcca gaattgtttg accaatatga aatctttaaa
    28801 tcacgtgaaa tgttgctaga atggtcacca aagaatgttc ataaagcaac aggtttggca
    28861 aaactaatta gccatcttgg aattgatcaa agtcaagtga tggcctgtgg tgacgaggcc
    28921 aatgacctct ctatgattga atgggcaggg ctcggtgttg ccatgcaaaa cgctgttcct
    28981 gaagtaaagg cagccgcaaa tgtagtgacg ccgatgacca acgatgagga agctgtcgcc
    29041 tgggctatcg aagaatatgt gctaaaggag aactaagata tgggattatt tgaccgtcta
    29101 ttcggaaaaa aagaagaacc taaaatcgaa gaagttgtaa aagaagctct ggaaaatctt
    29161 gatttgtctg aagatgttga tcctaccttc acagaagttg aagaagtttc tcaagaagaa
    29221 gctgtgcttg aagaaagtca ggattcagag ccagctgtag gggtttctca agaagaaata
    29281 gaagaattac cagaggaaga gaagcttgag cacgaaggaa ctatagaaga aaataattct
    29341 gaagttcttg aagcagaaag gcctcaagca gaagaaaccg ttcaggaaaa atatgaccgc
    29401 agtcttaaga aaactcgtac aggtttcggt gcccgcttga atgccttctt tgctaacttc
    29461 cgctctgttg acgaagaatt ttttgaggaa ctggaagaac tgctgattat gagtgatgtt
    29521 ggtgtccaag tcgcttctaa cttaacggag gaactacgtt acgaagccaa gcttgaaaat
    29581 gccaagaaac ctgatgcact tcgtcgtgtc atcattgaga aattggttga gctttatgaa
    29641 aaggatggca gctacgatga aagcatccac ttccaagata acttgacagt tatgctcttt
    29701 gttggtgtga atggtgttgg gaaaacaact tctatcggaa aactagccca ccgctacaaa
    29761 cgaactggta agaaggtcat gctggttgca gcagatacct tccgtgcggg tgcagtagct
    29821 cagctagctg aatggggccg acgagtagat gttccagtag taactggacc tgaaaaagct
    29881 gatccagcca gcgtggtctt tgatggtatg gaacgtgccg tggctgaagg tatcgatatt
    29941 ctcatgattg atactgctgg tcgtctgcaa aataaggata accttatggc tgagttggaa
    30001 aagattggtc gtattatcaa acgtgttgtg ccagaagcac cacatgaaac cttcttggca
    30061 cttgatgcat caacaggtca aaatgcccta gtacaggcca aagaattttc gaaaatcaca
    30121 cctttaacgg gaattgtttt gactaagatt gatggaactg ctcgaggagg tgtggttcta
    30181 gccattcgtg aagaactcaa tattcctgta aaattgattg gttttggtga aaaaatcgat
    30241 gatattggag agtttaactc agaaaacttt atgaaaggtc tcttggaagg cttaatctaa
    30301 tcagaagcaa aaatcctgca aggtacaaac ttgcaggatt tttttattcg aaacgaccat
    30361 cttgacgata ggcgatatct ggttgccaag tccatttggc atcgaatttt tcaagtaggt
    30421 caaagctggc ttgaggtccc atgcttccag ctttatagtc atgaagtggg gcaccatttt
    30481 cggcccagag ctcttcaata cggtcaatca acttccatga cgcaccaacc tcatcccagt
    30541 ggctaaagtt agttgagttg ttatttagga catcataaat caatttctcg tatggttctg
    30601 gagaagcacc agttgcagtc gcgtctgtac gataatcaag tgagttagga gccaagttaa
    30661 attcttctcc tacttgcttc ccatttaggc taagagagaa gccttctgtt ggttgaatat
    30721 agatggtcaa aatatttgga gcaagtggtt ctccaaagat agaatccatt tgtttaaaga
    30781 cgatgttgac atgagttcct ttttcagtca gtcgtttacc tgtacggaaa aagaaaggaa
    30841 caccacgaaa tcgatcgctg tctacaaaga aggcaccaga tgtaaaggtt tcagttgttg
    30901 attctggatt cacatttggt tcgctacgat aagagatgta tttcatgcca tcaatcttac
    30961 cagagcggta ttgtccacgg ataaagtgtt ctttgagttc ttcatcagtt ggatgataga
    31021 ggtttttaaa gaccttaatc ttttcagcac gaatctcgtc ttttgtgaag cttgctggtt
    31081 tgtccatggc gaggagcgaa agaagttgta gagtgtggtt ttggaccatg tcacggaggg
    31141 caccggattg gtcatagtag ccaccacgtt cttctacacc caagcgctcc gcaaaggtaa
    31201 tttgaacatt gtcgataaaa tccttgttcc aaacgttttc aaaaatcaag tttgcaaagc
    31261 gaactgcaaa gatgctttgg atcatttcct taccaagata atggtcaata cggaaaattt
    31321 gttcttcgtc aaatgttgct aggagttcgt cattcaactt gcttgcagtt gcgtaatctg
    31381 taccaaatgg tttttcaacg atcaagcgct caaaaccttt gccatcgaca atgttttcag
    31441 atttgaggtg tttggcaatg gttccaaaga actgaggtgc catagacaag aagaagagct
    31501 tattgtgttc agcttggtat ttttcattaa gctcagcttg taattgacgc aaagcaatat
    31561 aatgttccga atcattgaca tcatggcttt gatagtagaa gtggctagca aattcttggg
    31621 cttgctcggt actatctgcc aaatcaagga tggactcgac aactacagat tcaaaatatt
    31681 ccttactcca aggtctacgg gcagttccaa taacggcaaa gtgcttggaa agattgccgg
    31741 attgatatag tctaaaaagg gaagggtaga gcttgcgttt agccaggtct ccactcgcac
    31801 cgaaaattgt aacaataacc ttagatgaca tctagctacc taatttctat ttttattcct
    31861 agtcttgcta ggtatgagga aacctcattt cataaactta attctaacat aattttccga
    31921 aaaatcaaaa attccaatac gaggtagaaa aactgcaagg aaatccttac agtttgagtt
    31981 tagacgttta agaccttatc taagaactct ttcagacgag ggtgttgtgg gttatcaaag
    32041 atttggtcag gtgttccgtc ttcaaggaac tcgccatctg cagtaaagat aacgcggttg
    32101 gcaacctgac gagcaaatcc catctcatgg gttacgataa tcatggtcat gccttgctca
    32161 gccaattcct tcataacgtt aagtacgtct ccaaccatct cagggtcaag ggcagaagtt
    32221 ggttcatcga agagcatgat gtctggattc attgctaggc cacgagcgat ggccacacgt
    32281 tgtttttgac cacctgatag gctatctgga ttggcattag ctttatctgc tagtccaacc
    32341 ttttcaagca actccattcc caattcctca gcttcttcct tagtcatcaa cttgtgctca
    32401 ataggagcaa aggtgatgtt gtccaataca gacatatgag ggaagaggtt gaagtgttgg
    32461 aataccatgc cgatattttc acggacgtgg tcaacattgg ttgttttttc agttaaatca
    32521 tagccgttca cagtgatgtg accgctagtg acttcttcta aaagattgag gctacggagg
    32581 aaagttgact taccagaacc tgaaggaccg atgatacaaa caacatctcc ttcatagaac
    32641 ttagtcgtaa ttccttttag gacttcattt tttccatagt acttgtgtaa atcatttaca
    32701 tcaattttta attttgccat taacgaatcc tcttttctaa gcgtttcgct agtctagtca
    32761 aaagtgtgat aattacaaga tagaagatag caaggattgc atacatcttg aaactttggt
    32821 agttacgagc aatgataatc ttaccagttt ggaagagttc aaccaaaccg atagcagata
    32881 cgatagttgt atctttaaga gcgataacga attggttgac aaagtttggc aacatcaatt
    32941 tagttgattg tggcaagata atcttacgca tggtttttcc ataagagata cccaagcttc
    33001 ggctggcttc catttggcca actggaacgg cctgaatacc accacgaacg atttcagcga
    33061 tataagcagc cgcattgagt gagagggcaa ttgttccagc tacaaagtcg ttaattgggc
    33121 tttgttggcc tgtgatagac tcgatgaagt ttggaattcc ccagaagatg aaggctgcaa
    33181 gaatcatcaa tggaatacca cgaataacgt caacgaaaat ctcagagatg acgcgaagag
    33241 atttgtatgg gctaacgcta aacataccga agataattcc gatgacaatg gcaatagcaa
    33301 atgagataag agctagagca agagtgatac caagaccgct aaggagttgt ttgtagttgt
    33361 tttgaagcaa gccccagagc gttgtttcgt caacagtact tgttgaagca gttgaagatt
    33421 cgctagctag gtatttgtca agaatctttt ggaattcacc gtttgcttta aggtttgcaa
    33481 gtccgttgtt gaacatttca atcagttctg gatttgctcc ttttttaacg gcaaaggctg
    33541 tttcaccgat tggagttcca gagattggag ttttcaattt ttgaccttgg ctgatagaat
    33601 atttgagaac aggttcatca tccataacgg catcaatggc accagtgttt aaactgtcat
    33661 acattgaaga accatcagca aaggttttga ttttgtaacc gtatttgctt tgattttctg
    33721 ttaggaaggt ttgagaagca gttccgtttt taacaccgac tgtctttcct tttagatctt
    33781 cataagaagc aatattgctt gattctttga caccaagaat ggtattagca gtgtagtatg
    33841 attctgagaa gtcaaaagtt gccttacgag catctgtgac agacatacca gcaatgatac
    33901 catcggcttg accagcttgg acagcactga tagcagcatc aaaaccaggg ttggtgattt
    33961 caatttcaaa accttggtct ttagcgattg ccttaatcaa ttccatatca ataccagtgt
    34021 attggttgct tgaattttgg aaaacaaaag gtgcaaaaga agaatcgctg gcaatgatat
    34081 atttagcctt aacaggaata gcttttaatc ctgcgagagt agttgtagtt ggcactgctg
    34141 aagatgatga agcagtccat ttcttgataa ttttatcaag actaccatct tttttcattt
    34201 cagccaaggc ttggttaaat tcagtaacca ggtgctcgta tttacttcct tttttcacac
    34261 cgaaagcaaa acttcctaca gcttcaccat ccatttcaat atggaggtct tgaccttggt
    34321 taatggcata ttcgataaca ggtttgtcat ccatcatggc atcgatggca ccagcactca
    34381 agctgttgtt cattaaatca ccagtgtcaa atgttttaat agtaaagccg tatttatctt
    34441 tgattgtttc aaggaaacgt tgagcggcag ttccgttttt aacaccaacg gttttgccag
    34501 ttaattggtc gtacttgcta attttgtgtg actttgtagt agcaatgaca acttttgtat
    34561 catagtaagt atcagacatg gtgaagactt tttcacgttc tttagtcttt gtcatccctg
    34621 ccatgatagc gtcggcttgc ccagcttgaa ccgcattgac tgctgcgtca aatccaggat
    34681 aggacatctg aatgttccag cctttaatct cagcgacttt gttaataatg tcaacatcaa
    34741 ttcctttata agtttgatct gaatctttaa actcaaaagg tgcataggcg gtatcagaaa
    34801 caatcttaat cgtttctgct ttggcaatac ctaatgagaa aattgggaat aaaattagca
    34861 aaaatgctag aaattttttc ttcattttta gtctcctttt ccgaatattc tctcattata
    34921 tcagaaaaag taaaaaaagg caaattttta tatttttcgt gtcagaaaat ataacattga
    34981 tttttccttt tctttcttga attg
//
LOCUS       DAVKGU010000014        34969 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKGU010000014 DAVKGU010000000
VERSION     DAVKGU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34969
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.014"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(37..195)
                     /locus_tag="VW077_000614"
     CDS             complement(37..195)
                     /locus_tag="VW077_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU9454681.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLSWT
                     KVVQEKCK"
     gene            complement(267..716)
                     /locus_tag="VW077_000615"
     CDS             complement(267..716)
                     /locus_tag="VW077_000615"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9454682.1"
                     /translation="MVNCEQLEAYRQLEEAALVGCWAHVRRKFFEVPPKQADKSSLGA
                     KGLAYCDQLFSLERDWEALPADERLQKRQEHLQPLLEDFFAWCRRQSVLSGSKLGRAI
                     EYSLKYEETFKTILKDGHLVLSNNLAERAIKSLVMGRSKRVQWTLLA"
     gene            complement(789..1379)
                     /locus_tag="VW077_000616"
                     /pseudo
     CDS             complement(789..1379)
                     /locus_tag="VW077_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1303..1497)
                     /locus_tag="VW077_000617"
     CDS             complement(1303..1497)
                     /locus_tag="VW077_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9454683.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEEDGDVPS"
     gene            complement(1694..2017)
                     /gene="tnpB"
                     /locus_tag="VW077_000618"
     CDS             complement(1694..2017)
                     /gene="tnpB"
                     /locus_tag="VW077_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9454684.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
     gene            complement(1998..2183)
                     /locus_tag="VW077_000619"
     CDS             complement(1998..2183)
                     /locus_tag="VW077_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454685.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2487..4049)
                     /gene="guaA"
                     /locus_tag="VW077_000620"
     CDS             complement(2487..4049)
                     /gene="guaA"
                     /locus_tag="VW077_000620"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9454686.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4191..4889
                     /locus_tag="VW077_000621"
     CDS             4191..4889
                     /locus_tag="VW077_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9454687.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQISYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4932..5828)
                     /locus_tag="VW077_000622"
     CDS             complement(4932..5828)
                     /locus_tag="VW077_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9454688.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5837..6772)
                     /locus_tag="VW077_000623"
     CDS             complement(5837..6772)
                     /locus_tag="VW077_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9454689.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6769..7494)
                     /locus_tag="VW077_000624"
     CDS             complement(6769..7494)
                     /locus_tag="VW077_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9454690.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7578..8213)
                     /locus_tag="VW077_000625"
     CDS             complement(7578..8213)
                     /locus_tag="VW077_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9454691.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8215..9042)
                     /locus_tag="VW077_000626"
     CDS             complement(8215..9042)
                     /locus_tag="VW077_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9454692.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(9064..9990)
                     /locus_tag="VW077_000627"
                     /pseudo
     CDS             complement(9064..9990)
                     /locus_tag="VW077_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            10641..11252
                     /locus_tag="VW077_000628"
     CDS             10641..11252
                     /locus_tag="VW077_000628"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9454693.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11297..12025
                     /gene="rlmB"
                     /locus_tag="VW077_000629"
     CDS             11297..12025
                     /gene="rlmB"
                     /locus_tag="VW077_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9454694.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(12064..12975)
                     /gene="trxB"
                     /locus_tag="VW077_000630"
     CDS             complement(12064..12975)
                     /gene="trxB"
                     /locus_tag="VW077_000630"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9454695.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLIVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(13041..13265)
                     /locus_tag="VW077_000631"
     CDS             complement(13041..13265)
                     /locus_tag="VW077_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9454696.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13343..14086)
                     /locus_tag="VW077_000632"
     CDS             complement(13343..14086)
                     /locus_tag="VW077_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9454697.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(14086..14886)
                     /locus_tag="VW077_000633"
     CDS             complement(14086..14886)
                     /locus_tag="VW077_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9454698.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(15044..15400)
                     /locus_tag="VW077_000634"
     CDS             complement(15044..15400)
                     /locus_tag="VW077_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9454699.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15413..15925)
                     /locus_tag="VW077_000635"
     CDS             complement(15413..15925)
                     /locus_tag="VW077_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9454700.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15925..16419)
                     /locus_tag="VW077_000636"
     CDS             complement(15925..16419)
                     /locus_tag="VW077_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9454701.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16554..17000
                     /locus_tag="VW077_000637"
     CDS             16554..17000
                     /locus_tag="VW077_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9454702.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16997..17644
                     /locus_tag="VW077_000638"
     CDS             16997..17644
                     /locus_tag="VW077_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9454703.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLVIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(17665..18246)
                     /gene="pdxT"
                     /locus_tag="VW077_000639"
     CDS             complement(17665..18246)
                     /gene="pdxT"
                     /locus_tag="VW077_000639"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9454704.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18247..19122)
                     /gene="pdxS"
                     /locus_tag="VW077_000640"
     CDS             complement(18247..19122)
                     /gene="pdxS"
                     /locus_tag="VW077_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9454705.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19274..20653)
                     /locus_tag="VW077_000641"
     CDS             complement(19274..20653)
                     /locus_tag="VW077_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9454706.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20947..21870
                     /locus_tag="VW077_000642"
     CDS             20947..21870
                     /locus_tag="VW077_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9454707.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21930..22535
                     /locus_tag="VW077_000643"
     CDS             21930..22535
                     /locus_tag="VW077_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9454708.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22553..23797
                     /locus_tag="VW077_000644"
     CDS             22553..23797
                     /locus_tag="VW077_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9454709.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNIANEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(23893..24150)
                     /locus_tag="VW077_000645"
     CDS             complement(23893..24150)
                     /locus_tag="VW077_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9454710.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24192..26228)
                     /locus_tag="VW077_000646"
     CDS             complement(24192..26228)
                     /locus_tag="VW077_000646"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9454711.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26440..27357)
                     /gene="glyQ"
                     /locus_tag="VW077_000647"
     CDS             complement(26440..27357)
                     /gene="glyQ"
                     /locus_tag="VW077_000647"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9454712.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(27725..28486)
                     /locus_tag="VW077_000648"
     CDS             complement(27725..28486)
                     /locus_tag="VW077_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9454713.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLS
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(28759..29601)
                     /locus_tag="VW077_000649"
     CDS             complement(28759..29601)
                     /locus_tag="VW077_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9454714.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(29715..31106)
                     /locus_tag="VW077_000650"
     CDS             complement(29715..31106)
                     /locus_tag="VW077_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9454715.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDVSKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(31341..31718)
                     /locus_tag="VW077_000651"
     CDS             complement(31341..31718)
                     /locus_tag="VW077_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9454716.1"
                     /translation="MLCQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGTQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLASHSFIVK"
     gene            31969..32946
                     /locus_tag="VW077_000652"
     CDS             31969..32946
                     /locus_tag="VW077_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9454717.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFADHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            32943..34025
                     /locus_tag="VW077_000653"
     CDS             32943..34025
                     /locus_tag="VW077_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9454718.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFELLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(34089..34969)
                     /locus_tag="VW077_000654"
                     /pseudo
     CDS             complement(34089..34969)
                     /locus_tag="VW077_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT        10305 a         7833 c         6331 g        10500 t
ORIGIN      
        1 agagaactca cggatagttc ctaatctgga gatttcttat ttgcactttt cttgtacaac
       61 tttagtccac gataaatagg cctctaaaac ctctttgttt acgagagttt cctcgtttgg
      121 aagacattct agaagatagg atagatattt ctcactattt agttgatgac gtttagctgt
      181 ttccaataag ctcataataa tagctcttgc tttagctcct tcaaaaactt tgagaaaaca
      241 accaccctcg cttttttaaa ctgagcttag gctaaaagag tccactggac tcttttactc
      301 cgtcccataa ccaatgattt aatggcgcgt tcagctagat tattggaaag gaccagatgt
      361 ccgtctttta aaatggtctt aaaggtttct tcatacttga ggctgtattc aattgccctt
      421 cctagttttg aacccgataa aactgactga cgacggcacc aagcaaagaa gtcttccagt
      481 aggggttgga gatgttcttg acgtttctgt agtcgttcat cagctggcaa agcctcccag
      541 tctctttcca aggaaaataa ctgatcacaa taagctaaac ctttagctcc taaggatgat
      601 ttatctgctt gcttgggggg cacttcaaaa aacttccttc tcacatgcgc ccaacatcca
      661 acaagtgcag cttcttccag ttgacgataa gcttctagct gttcgcagtt taccaagctt
      721 agcgtcgcct tactcctaaa ctttggactg ctatcgcata ggcagaacta gaggactaaa
      781 gtcctaagtt actgccgcaa catgtcacaa tgaacatagc cagaataatc tcctaggaat
      841 tcttgtacta ctaaaccact ccgacgctga tcatggtggt acagcgtgat tgcttgattc
      901 tcagctttcc cagacaaaaa agtccaatag taagtcaact gactatcact ctctagaacc
      961 cgataagagg tttcatccgc atgaagtaag ggcttgagtc aatagtctcg ttataaaggg
     1021 gctttaggaa taggagcttt cacgatttta tcactcggat ttttatcact gcatgcttgg
     1081 catttgtaag cgtgttggat atgatctatg cgttttaatt gcgcaggaat aaagaccaat
     1141 tcttgtcctt gaagactccc tccaatctct tttagatctc cctgacaatc agggcaaatc
     1201 ctctcttcta cttgatgatg aacttcttct gaatcaaatt gggcaagaag agcttggcgt
     1261 ttccctttag atttcttacg tttgtaggta atttcctctg tttcaactgg gaacatctcc
     1321 atcttcctca gatggagact cttcctcaaa aaggttgagt tgtccagagg ggcaaacact
     1381 tttctcagag gattttccat agagcttttg agtcagataa gccacttgtt cacgaagaag
     1441 ggcaagttca ttggtgagac tatcaattgt agcactctgt tgttgaatga ttttcaatct
     1501 tcttccatga gcttccctct ctgttttcta actttattat actaaaaaga aagtcctcat
     1561 ttcaatagaa atcacgactt tctgatggat ttatagtaaa atgaaataag aacaggacaa
     1621 atcgatcagg acagtaaaat cgatttctaa caatgtttta gaagcagagg tgtactattc
     1681 tagtttcaat ctactatatt tttggagtga tagaaaagcc tttcatcagc caatctactt
     1741 gttcaggtgc gagagctttg acatcctttt ctgtactggg ccaagtcagt ctgccgttct
     1801 caaagcgttt atatagtagc caaaatcctt gaccatccca gtaaagggct ttaaagcggt
     1861 ctttacgtcc accacaaaag agaaaaactt gaccggagaa aggatccaat tcaaagtggg
     1921 ttttaaccag ataagccagt gaatcgattc cttgtctcat atcagtcttc ccacacacga
     1981 gatagacctg ccctaggcta gatagatgaa ttgtcataga gcaacacctt atccaaaagc
     2041 tgttctacca tttcgagatt gagagattga aaaaaactta cttcaagctt gccaatacga
     2101 attttaagca gaatatcatt tctcttttta gaatcaaaag gacgagattg tggaatctct
     2161 acaggaacaa taggttgtgg cattaaaata tcctccaata gttttacttt actaatagta
     2221 tacaggagga actccatgaa tgatagatac cttgttatga cgcgcttact atattagaaa
     2281 gttggctctc taattaaatt aattctcatt atatatatca aaagaccgca gtttgaaaac
     2341 tacggtctaa ttttttgtcc aaatgtaaat cacttggaac tattattcaa tttattcttt
     2401 ttagtaaatg ttcccgtttt aatcccagtt acagttttta atcctctgta accgttgatt
     2461 ttacaaagct tttaattttt ttgcgattac tcccactcaa ctgttgcagg tggtttactt
     2521 gtaatatcgt agacgatacg gttaacatga tccacttcat ttacgatacg cactgagatt
     2581 ttttgaagta cttcccatgg aattttggca aaatcagcag tcataccatc gatagaagtg
     2641 atagcacgga ttgcaatcgt gtagtcatac gtacgaccgt cacccataac accgactgaa
     2701 cgaacgcctg tgttaacagt gaagtattgc caaatatcgc ggtcaagtcc agctttagcg
     2761 atttcttcac gaagaatagc gtctgattca cgaacggttt caagtttctc ttcagtgatt
     2821 tcacccatga cacggatagc aagtcctggt cctgggaatg gttggcgcca tacgatatgg
     2881 tctggcatac caagctctgt accaagagca cgaacttcat ccttgtaaag agtattgagt
     2941 ggttcaatca attcaaactg catatcttct ggaagaccac ccacgttgtg gtgtgacttg
     3001 atagtttgag ctgtatccgt accagactcg ataacatctg tatataaagt accttgagca
     3061 aggaatttca catctttgag cttgcttgct tcgtcatcga atacatagac aaactcgtta
     3121 ccaatgattt tacgtttttg ttcaggatca gaaacgccag caagtttgtc aaggaaacgt
     3181 ttagcagcgt ctgctttgac gatattcaaa ccaaacttac caccgagcat gtccataact
     3241 tgatcagctt cgcctttacg aagaagaccg tggtctacga agatacagat caattgatcg
     3301 ccaatcgctt tttggagaag aaccccaacg acagatgagt caacaccacc tgatagacca
     3361 agaaggacac gtttatcacc gacggtttca cgaatttttt tgatctgcat gtcaatgaaa
     3421 ttatccattg accagtcacc tttagcctta caaatgttaa gggcaaagtt acgaaggata
     3481 tcatttccgt atacagaatg acgaacttct gggtggaatt ggataccgta aatgtgttta
     3541 tctgggtttt cgatggctgc gtatgggcag tcagctgatg tacctgtacg aacaaagtca
     3601 gcaggaatct cagtaaccgc atcaccatgg ctcatcaaaa cagtctgttc atcaggtgtt
     3661 gattcaaaaa gcgctgatgg tgtgtgagtt agggttgatt gaccgtattc acgatttcca
     3721 gcatcacctg caggaacaac ttttcctcca agtttatggg tcaataactg cataccataa
     3781 cagattccca aaattggaat tccgagttcg aagatttctg ggtcaatatc aaatgaacca
     3841 tcttcatata cagaatttgg accacctgat agaataattc ctacaggatt gacttcacga
     3901 acttcagcag ctgaaatttt atggcttttt agttctgaaa aaacaccaat ctcacggata
     3961 cggcgtgaaa tcagctggtt gtactggcta ccatagtcca atacgatgat tttttctaca
     4021 tcttgcaaat cagttgaaat gttgctcatc tttttccttt ctcaaaagaa atatcttttc
     4081 tcaatattct atcacaaata agggcgaatt accaactaaa caaggcgaag tttgactttt
     4141 tctagtttat tggggtacaa tagagaaaag atagaaatga agtgaaaaat atgctacctg
     4201 cttatatgaa aatccatgat cagataaaaa aggatattga cgagcaccgt tgggctattg
     4261 gtgagaggct tcccagtgaa agagatttag ctgagcagtt tgcggtcagt cgcatgaccc
     4321 tccgccaagc cgtatctcta ttagtcgaag aaggcgtctt agagcgccgt gtaggaagcg
     4381 gcacctttgt ttccagtact cgagtacaag aaaagatgcg agggacaacc agttttactg
     4441 aaattgtcaa atcccaaggt aaagttccct ctagccagct catttcctac aaaaaaacca
     4501 ttcccaatga gcaagaagtt gccaagctag gaatttttcc aacggacaat attatccgaa
     4561 tggagcgggt ccgctatgcc gaccaagttc ccctagttta tgaagttgct tctattcctg
     4621 aaaaattcat taaggacttt aaaaaagaag aaatcaccag tcatttcttc caaaccttgc
     4681 aaaaacatgg ctatcgtatc ggtaaatccc aacagaccat ctatgctcgc cttgctaaag
     4741 aaaagattgc ccactatttg gaagttgaaa aaggacatgc tattcttgga ttgacccaga
     4801 tttcctacct agaagatggt actgcttttg aatacgtaaa aagtcaatat gtaggcgaac
     4861 gctttgaatt ttatcttgaa aataattaga ttaccacgca aaaactctct gtcacggaca
     4921 aagagtcttt tttatttttc taagaataaa tctttcaaag aaatcaaagt tacagcaaac
     4981 aatatcattg ccataccaag aaaatcaatg ggataaaatt gttcattcat tattaagaag
     5041 gcaaagaaaa tcgccgatat tggctcaatt gaagccaaca agcttgactt gaccggtcct
     5101 atcagactgg ctcctttaag gaaagctgta taggcaaaga cagtcccgat aaggataatg
     5161 cctgcaaacg caaggagaaa atcaagacta gtcgggatat cggcctgtag aacccctgta
     5221 aaaggaaggg cgaccaaacc tgctatgacc attcccacac caatgaccaa gctgctcccc
     5281 cactttttaa tcaaggctat gggtaaaatg atatacagag cataagtcaa ggcagaaaag
     5341 agaccccaga acagaccagc aggtgtcatg gataactggt ccaactgccc atgtgttgcg
     5401 atcaggaagg ttcctccgat ggcgaatatg atggaaacta tctctcccag tgtcggtgcc
     5461 accctatcct tgatacagct ataaattaaa attccgacag gacaaacata ctgaagcacc
     5521 gtcgctgttc ccgcattggt ctcctgaata gcagacagat aggcgaattg gttgagaaaa
     5581 agaccaatca gagcaaaaat aagaagagac agcaaactct ttctatcctt taaaaagacc
     5641 agtattttat cctttgcagt agcataagcc aagagcatga gaattccacc agcgattaaa
     5701 agacgcaagt tagtcaagac cagagccgaa attccgtgtg ccattaggta ttggccactc
     5761 gttcctgaca acccccaagc aataccagcc acaactgtta gtaaagtccc ttttaaacta
     5821 tttgacatgt ctctccttac tcttctttgc gaatcaagtt catcacttga ttgcggtcta
     5881 atatccattg agctctctcg tccttttcat aggtacgatt cgataggaaa atagtggctt
     5941 cttgcttctg acgattccac atgataaagg tacctgtata gcccgtatgg tctagccaat
     6001 ctccttccaa attccatgct aaagaacgtt ccttgtcatc caaaggagaa aaattttgat
     6061 ttaagtctct tgcaaaatca tctgctaaat agtgttctaa aaagatttgt aaatccttta
     6121 tagtcgaaaa taaaccagca ctcccagcat gtctacccag gagacgagcc ttgggatcat
     6181 gcactatgcc tgcctctaca cctctaactg ttggaacagc aagctcaact ggcccaaact
     6241 tggtttccgt cattccccaa ggtttccaga cttgatcctt taaaatcaca tccaaatctt
     6301 gattaaaaat tctttccaaa ataaagccca acagcaaaaa atggacatcc gaataaagaa
     6361 aggctggctg acttcgtctg ttgagatgaa acatcgcttc ctttaattca ggggctgtta
     6421 aaagatcacg attaggaata aaaggatcaa ggtctgttgc atgagtcaag agctggcgaa
     6481 tagtgatgtc tggataatca ctctcaggta aaaaatctat taccagtcta tcaatatcta
     6541 attgacctat ttcccacaag aaggtacaaa ctgtgccaac cccaacaacc ttgctgacac
     6601 tagctaggtc ataaactagt cctgcctcag tctgcaagcc atgctctggg tcactctggc
     6661 ctaaatagaa ctctgtccat tgattgtcct taaaatacgc aaaagaggct ccgggataaa
     6721 tccctgcctc gatttgttct tctatttttt taataatctt ggtccacttc atacttcttc
     6781 aaaccacaat tcaacattat ttctatcttt accaaggaag aatttttcag acttaggaat
     6841 aaaatattca gtagactcaa acttctggcg aagacttgct atgtctaatt cattgaccaa
     6901 gaacttgagc atagataagt cccaggtaac cgtattgtcc acagtcaaat cctgcccctg
     6961 agctggaata aaatcaaggg atgccccaat ttcagatgat tccaagaaac tttcgatatc
     7021 agttgggaga tgtaattcca tagaaatctc aaatttactt aaagaaattg atgccaaatc
     7081 tgtttgaaat tcaggctttt ctcctacttc tactagactt gctatgtcat cttccgcatg
     7141 aatcaaaatc aaatcatctt ctggtgagaa aatttcaaaa gcgtagccat tttgaccttt
     7201 atataatcga tgaatcgaat ctgttttaga taagattcct tcaatttcta agggattttc
     7261 caccttgaca atcaatctag ctagtttttt tcttccctct accttacgag tacgcatact
     7321 gggagcttct tctaaaacca gcttttcaag acccgtttgg tcacctagtg acagaaaggc
     7381 cgattcttct aacaaggcct tcattccaag ggtttcaata taaaatgttt catttaattt
     7441 tctattatta gcttttaaag taggaataat ccgtacaatc tgatttacat tcataaattc
     7501 ctccgtcact ttttatttta aaggatttta gaatttttta caagatatta tgctcaaatt
     7561 taacattatt tagctattta gtaaagatag tctttcaagg attttgacaa agcctgataa
     7621 ccagcaatac tgaggtgcag tccatcagtt gtatattctt ttttgagttg gcctgcttgg
     7681 tctgtcaaac aatcaaatac tggcacaaat tccacctgca tataggcaga tgcaagctct
     7741 tgataggctt gattccagtt ctgaattttt tcattcgagc ggatatagac tgcctgctgg
     7801 tactcctctc tctcattgac aggcaaaatg gaaagcaatt taatctctgt caatggataa
     7861 tcgcgagcaa cggattgaat gatagcttcg agattattga gagcctcatt cacaggaaca
     7921 tcctttccga tatcatttgt cccaatcaga agaaaaattt tatctactgc tccaccatat
     7981 agatgagcat caaggttctc tagtaacagt cctgtctgat agccacgaat tcctcgattg
     8041 acaatcgtct ttgaagtccc aaatagctcc tgtagaggat aatactcgac aatggaatcc
     8101 ccaataaaaa gaatgtttgg ttctacaaca gaaatgtgat ttaggtgacg atacttagtt
     8161 tgaatttttt cttgttcctt taggagccaa ttttctaata actgtactgc cacttcattc
     8221 tcctttttct aaccagtttt ccaagacttg ataaactcct ccttggctgt tggctggtgc
     8281 tagagcagtc gccacagctt tggctttctc atcagcattt tccatcgcat aggcaattcc
     8341 agccatttca agcatttcaa catcattttc actatcacca aaagccatga tttcttggga
     8401 tttcaagtcc cagcgcttga gtaattcctc caagccccat gctttatgaa tcccagcttg
     8461 gaggatatca atgcaaccat agccactgga tacagctcgg acacggccat caaagagagc
     8521 attgatttct tccaaaaccg aactcaaacg ttcctcacca acaaccatgc tcatcttgag
     8581 cacaccacca aagaggtcag atgttaattc atccacaaat tgcatccgtt ggtagaattt
     8641 ttcaatcatt tctggagtca taaaactttc aagatctgta aaaatcgtac cttccttgac
     8701 aaaatcaccc ttcatccccg ttacaacaaa ctggtcctga cacgctcgac ccttgaaatg
     8761 agtcaaagcc ttgttgacaa tggcgtcatc ccatgtctga gcctgaatca attcattgtt
     8821 ttcaaaaata cgagcgccat tagcaacaac cagaaccact cgatccacca agggactcag
     8881 tagttgtctc atgcggtgaa tttcattgcc cgtcgcaatg acaaaacgaa tgcccctttg
     8941 atccaactga tctaaaatct tttccaatcg tgggagatca agctgacctc tagcatccag
     9001 caaggtccca tccatatctg tcgcaattac cttaatcatc atttactttc ctctggattc
     9061 aagctaatac cacttcaatc ccacatgttc attcatttct ttataggctg tatcaattcg
     9121 ttccttagtc gcttcatcta actggtcacg atgactgcca tcctctataa aatcttccaa
     9181 aatataagtt tggtaatgat acaagggata tccttctggt gaaaaggttc cctttggtgt
     9241 tcgattgacc caagtaggat gagctttagc tgttccgata gttgtttttc catccttctt
     9301 cttgatggtg acatccatga gaacaccacg ttcagtccac ttagcattct cttcatctcc
     9361 cataaattca attcgttgat tggaaatgaa gttccccatt taatagataa tgagtttctt
     9421 atctccatct ttttcaaccg tttcagatgg ttcaacaacg tgaggatgcc ctccaaagat
     9481 aatatccgct cccaaatcga tcatcttgtg ataaagagct ttttgttctt cagttggttc
     9541 caatcgatac tcaacaccca tctgaagcat gataatggtg atatctgctt ccttctctgc
     9601 ccgttcaatt tcaaccttta tcttatcttc gtttaaatat gaaagataac gattatagtc
     9661 ttcctgagaa atatactgct caattccatt gaaaccatag gaataggcta acaatgcaac
     9721 cttgatacca ttcacttcct taatgaccag cggagcctga tcacgtggtt cgtgcgtata
     9781 aactccgatt ggagtgattc cagctttctc aataatatcg gccgttgaaa taactccctc
     9841 aatttgcgaa tccaaaatat gattatgagc taaatccagc acatgataac ctgcctcctt
     9901 aatagcatcc ataacttcaa caggagcatt aaagagaaga taacccgcta aataatgatc
     9961 cttattaatg gttccttcaa aatcaccaat agctaaatct gctgcttagt ccccttgctt
    10021 gagccaagga gtcacatact caaaattttc atgaaagtca taggtaccgt cttctttttt
    10081 agctgaaaag aaaagtccat cgtggtagag caaatccccg tgggccataa ttctggcagt
    10141 tttttcctct tcctactcct gagacttttg cttagtcccc tcttgagaaa tagtatctcg
    10201 tttttgacta gtcaagggat tccttggaag ctttcaaaca acaagaccaa gcccattgat
    10261 aaaccaactg ctagcaagag tatcgccaca aatcccttat tgctccactt gcgataactc
    10321 ctaaaaagtt taccaagccc ttcataaaac gaaaagctaa accaccctga tttcgatttt
    10381 gtcttctttg tatcttcgtt ctccctactt tcttatgcaa gccttttctt tttattatat
    10441 cacagataag tatttctttc acaattgaat tgaacttccc atctattttc tataaatcct
    10501 aaatgccata atgctttcaa ttcttgtcat tttgtgatat catgtagagg aaatgaacta
    10561 atccacagtg gcttattcca agtataccac ttgggctttg gcagtagcta actgcactaa
    10621 acataatata aggagagaaa atgtctgcaa tagaacgtat tacaaaagct gctcacttaa
    10681 ttgatatgaa cgatattatc cgtgaaggga atcctactct acgcacggtt gctgaggaag
    10741 tcactttccc cctatctgac caggaaatca tcctaggcga aaagatgatg caattcctta
    10801 aacattccca agatcctgtc atggctgaaa aaatgggact ccgcggtggt gttggactgg
    10861 ctgctcccca gttagatatc tcaaaacgca ttatcgctgt tttggtacct aatattgttg
    10921 aagaaggcga aactccacag gaagcctacg atttggaagc cattatgtac aatccaaaaa
    10981 tcgtctctca ctctgttcaa gatgctgctc ttggcgaagg agaaggttgc ctgtctgttg
    11041 accgtaacgt gcctggctat gttgttcgcc atgcccgcgt tactgttgac tactttgaca
    11101 aagatggaga aaaacaccgt atcaaactca aaggctacaa ctccattgtt gttcagcatg
    11161 aaattgacca cattaacggt atcatgtttt acgatcgcat caatgaaaaa gacccatttg
    11221 cagttaaaga tggtttactg attcttgaat aaagaaaatc ccgttgcaag acggggtttt
    11281 gtgttataat agaggcatga aaacaaatga tattgtctat ggtgtccacg ccgttaccga
    11341 agctctcctt gcaaatacag gaaacaaact ctacctccaa gaagatctcc gaggtaagaa
    11401 tgttgagaaa gtcaaggaac tagctacaga aaagaaggtg tccatttctt ggacatcaaa
    11461 aaaatctctc tctgagatga ctgaaggtgc tgttcatcaa ggttttgttc tacgagtgtc
    11521 tgaatttgcc tatagcgagc tagattacat ccttgcaaaa acacgccaag aagaaaatcc
    11581 acttctattg attctagatg gtctaaccga tccccataat ctgggttcta tcttgcgaac
    11641 agccgatgcg accaatgttt caggtgtcat cattcccaag caccgtgctg tcggagtaac
    11701 tcctgtcgtt gccaaaacag ccacaggtgc tattgaacac gttccaattg cccgagtgac
    11761 aaacctcagt caaaccttag ataaactcaa ggatgaaggc ttctggacct ttggaacgga
    11821 tatgaacggt actccttgcc acaagtggaa tacaaaaggg aaaatcgccc tcatcattgg
    11881 aaatgaagga aaaggtatct ctagcaacat caaaaaacaa gtcgatgaga tgattaccat
    11941 tccgatgaat ggacatgttc aaagccttaa tgccagtgtt gctgcggcca ttctcatgta
    12001 cgaagttttc cgaaatagac tataaaaaag tttccagtca tctgattgga aactttttta
    12061 tgattaacta tgttctgtga taaacttgta ggcttcttga ccagcgatag ctccatctcc
    12121 aactgctgtt gttacttggc gaaggtcttt caagcgaaca tctccaactg caaagatacc
    12181 gtcgactgca gtcttcatgt ggctatctgt cacaatccag cctgcctgat cttggatatt
    12241 caattcttta acaaaatcgc taagagggtc caaaccaaca tagataaaga caccaccgaa
    12301 ggcttgttct gtcacttgac ctgttttcac attttcaaat acgactgatt ctactcggtt
    12361 ttcacccttg atttccctta ctacagaatc ccagataaag ctgatttttt cattcgcaaa
    12421 ggcgcgatct tgtaaaacct tttgggcacg aagttggtca cgacggtgaa caatggtaac
    12481 agtcttagca aaacgagtca agaagagggc ttcttcaaca gctgaatctc caccaccaac
    12541 tatcaataaa tcttggtcac ggaagaaagc accatcacac acagcacagt aagaaacacc
    12601 acgactgttc agttcttctt ctccaggcac tcccaaagga cggtgtttag aaccagttgc
    12661 tacgataact gtacgtgttt catatgtttg gtcatcagtc atcactttct taaaatcacc
    12721 atggtcttcg acattttcaa cataaccata aatgtgctca acaccaagat tttcaagtgg
    12781 ttcaaacatc ttttcagcca attcaggtcc actaatatta gcgtatcctg ggtaattttc
    12841 gatatcagat gtattattca tctgaccacc tggcagacca ccttcaatca aagctacttt
    12901 tagattgctt cgagcagcat acaaggccgc agtcatccct gcaggtccag caccgataat
    12961 aatagtatcg tacatataga ttccttcttt cttgttgtaa ctatctttat tctaactctt
    13021 tcttgtcaat cacgcaagga ttatgccttg aaaacaagaa ttaaactcat aaccgcaaag
    13081 gataatactg ggacaaccaa gactccaatc ataatcatat catagagatg ggcttggcga
    13141 gccttggctg tcttatcaac tcccgacatg gctctcagac caatccaaat ccctaaaaaa
    13201 atcagaacaa ggatggtggt caatatcaaa ctctcgaaat ataaagaaaa tagttgcagt
    13261 agcatgattt ctctcatttc tatctttttt aaagagtaaa ctcagctagt ccaactaact
    13321 gagttttcct ttatctatta tatcaaatat aagtccgttt gtaactagcg aagaattctt
    13381 ttgtccgctc ttctttaggg gtgtggataa tctcatccgg agttccagac tcgatgattt
    13441 tccccttatc taagaagaga atcttatccg caacttgggc tacaaaggac atgtcatgac
    13501 tgaccaaaat catggtctga cctgacttag cagcatctgc aatagacttt tctacttcac
    13561 cgaccaattc tgggtcaagg gctgaagttg gttcgtctaa gagcaaaaca tctggtttca
    13621 tagcaagcgc acgcgctagg gcaacccgtt gcttctgtcc acctgataaa tggcgaggat
    13681 aatggttttc acggtccgaa agcccaacct tagccaactc ttccttggca atcttagtcg
    13741 cttcttggtc agataatttc ttgacaacaa ccaagccttc tttcacatta tcaagtgctg
    13801 ttcggcgttc aaacaaatta aactgttgga aaaccataga caacttacgg cgtagggcaa
    13861 ggatttcttc ttgagtgatt ttagaaaaat caactgaaaa accatcaatc tgaatagagc
    13921 cactgtcagg tgtttcaaga taattgagac tgcgaagaaa ggttgatttt ccagctcctg
    13981 aagaaccaat caaggctaca acttcccctt tttgaatatc caagtccaga tgatccaaga
    14041 cagtctgtcc tgaaaaggat ttgcttaaat tcgaaatctt aatcattaac gaaggtctcc
    14101 tttcacatct gtttgcactg tatcaggtgc agaaatagcc atttttctct cgatgaaacg
    14161 accgaggctt tcaattccga tattgactac ccaataaaca agggcaacgg agatgaagcg
    14221 ttcaaaatag cgataatcag ctccacctag aatctgagct tgggcaaaga cttccacaac
    14281 acccgcacta aaagctagag atgttccctt ggtcaaaccg atgagggaat taatcaaggt
    14341 tggagtagct accaccgctg cattaggaat aatcactcgt cgataaactt gcgctcgggt
    14401 catacccaga ctgcgtgccg cctcaatctc accaggatta actgagagaa tggctgcacg
    14461 aatggtttca ctagcataag ctgcctcatt aaaggcaaaa gcgacaatcg caaaagctgc
    14521 agctggaatc gcattgatat tgagaccagt tccccattgc tgattgaggg ctttcaaagc
    14581 caaagggatt ccgtagtagg tcaacatgag ttgcaccaaa atcggtgtcc cttttaagaa
    14641 actaacaaag aaggcctgca agggatataa aatcttgaca cgattgatct tcacaatggc
    14701 aaaaagaagc gccaaaacca agccaaaaag ggcaccacca attgtcaaca taattgttgt
    14761 tggaagttgt tggacaattc tagggattcc atcaaagacc gaacgtaggc taaacagctt
    14821 gccatccgga atcaattgca tcaagttttg gtaccaatct gatgctaaaa tcgttgtaac
    14881 attcataaaa acatcctctt ctgataatga ttcattctac catgcttctg ccgtcaatga
    14941 aattatttgc tacggtcaga tacagacttt gtctacctac tagtttatca tactttgaaa
    15001 cagggaagtt atatatttta tctatcaaag agatagataa aaactatttc agtcccagtt
    15061 cttcataaga ttttcgataa ccgatttgct taacagttcc attttctact aaaatgggcc
    15121 gttttaacaa cataccgtca cttgctagca actcagccgc ttcttggttt gacaaacttc
    15181 ctaccttatc ttttagccct aattcacggt atttgatacc actggtgttg aaaaattgct
    15241 tcaattcaaa tcctgaggtt tctagccaat tcaaaatgac ttcttggcta ggtgtttctt
    15301 ccacgatatg gacggcttta tagtccacac ctaattgatt taattcttgt tttgcttttt
    15361 tacaagttga acatttgggg tattcgataa attctaacat gtgcttctcc ttctatttgt
    15421 ttatatcttt aaaatctacg ccttcatgct ccaagagcca agctttcttt tccactcctg
    15481 cagcatagcc tgtcagacgc ttgcctgctc ccaacacacg atgacaaggt actaggatag
    15541 accaaggatt gcgtcccact gctccaccaa ttgcttgagc agaagccact tgcaggtctt
    15601 gagcaatttg tccataggtc actgtctgac cataaggaat gccctgtaaa tagtcccaaa
    15661 ctctcttttc aaaattcgtt ccgattggcg ccaagagcaa gttggataaa tcctgaggct
    15721 tgcctttaaa gtaatcatct aagcaagcaa taactgggtc taaaatagga tgactaacaa
    15781 cttcttctat cgtttcatct cctagtcccc tctcaaaatg cttctgctcc tgaacccaaa
    15841 ttccatacaa ataatggtca tcagctacaa gtgatagaat accaattggt gaggagtaga
    15901 gtatttttac atacttcttt tgcattattt ctttaatttt tgataggcca agcgttcgcc
    15961 atctactggc accttaccaa cttgtttaaa accaagtttt tcaaaaatat gttgcataac
    16021 cttgttttca gcatgcgtat ctgagcgaaa atcaagataa tcaaaacctt caatcaagcc
    16081 ctctaagaag gtttgagcaa ctccttttcc ctgcacatct gctgccacag caatacggtg
    16141 aaagactaga tactctgact ctccagcttg ccagtttccc tcataaatag cttcataggc
    16201 ctcctctgga ctcttggtca cagcagcata ggctagcagt tctccctctt ccaaggctac
    16261 gtaggcttgc cctgagatga tatcatcgat aataacatct gcatttggat agccattttg
    16321 ccactggtca ctaccagcat ctgctaaaca ttttttggca tcctccatca cctgcatgat
    16381 ggcatctact tcatttggaa aagctaaacg aatttccatc ttttttcctc atttctgact
    16441 agtttctccc atcatagcat aatttaagta tttctacaag cagttaaaac ttgactttgt
    16501 gtttttgtta ttttataatc tagttatcaa aactatataa aaaggagttg aaaatgaaaa
    16561 ctaccttttc ctacccaaaa tgggcagaaa ttccaaacat tgacctctat ctggaccagg
    16621 ttttgctcta tgtcaatcag gtctgcgccc ctatctctcc taataaagac aagggcctaa
    16681 cagcatctat ggtcaacaat tatgtgaaaa atggttacct gacaaagcct gacaaaaaaa
    16741 aataccaacg ccaacagatt gcccgtttga ttgctatcac aaccctcaag tctgtatttt
    16801 ctattcaaga aatagcccag acacttaaca ctctacaaac tcaagcaagt tcagaccaac
    16861 tctacgatgc ttttgtggac tatatgaacc aaggaattga cccagctaac cctattatcc
    16921 aaaccagctg ccaaaccgtt aaactctatc atcaaactct agacttaatc gatcatactc
    16981 aagaggaggt aatccaatga acactagtct taaactcagc aaacaactca gttttggaga
    17041 ggagattgct aatagcgtga cccatgctgt gggtgcagtc atcatgctta tcttgctgcc
    17101 tatttcatcc atctatagtt atgaagcaca cggattttta tcctctatcg gcgtttccat
    17161 tttcgtcatc agtctctttc tcatgttcct atcatccacc atttatcact ctatggccta
    17221 tggttcgacc cacaaatatg ttttgcgaat cattgaccat tctatgattt acgttgccat
    17281 tgccggctca tacacgcccg ttgtcttgac cttgatgaat aactggtttg gctatctgat
    17341 tattgtcatc caatggggaa cgaccatctt tggtattctc tataaaatct ttgctaaaaa
    17401 ggtcaatgag aaatttagcc ttgctcttta cctgattatg ggctggttgg ttctggttat
    17461 cattcctgcc attatcagtc aaacgacacc cgttttctgg agtctcatgg taactggcgg
    17521 actctgttat acagttggag ctggatttta tgccaagaaa aaaccttatt tccacatgat
    17581 ttggcatctc tttatcctag ctgcgtccgc acttcaatac atcgctattg tttattacat
    17641 gtaaaaaagt tgagaaattc aatctcaact tttttcttta cacatattga taaagtactg
    17701 gtgcaagcgc acatcatcag tcaattctgg atgaaaagaa cttaccaaca tatttttttc
    17761 ttgggctgca acaatttgat tgttcactgt tgctaaaatt tctacaccct caccaacact
    17821 actgataatc ggaccacgga taaaggtcat tggaatcttg ccaactccct tacattctgc
    17881 ttccgtgtag aaacttccta attggcgccc ataagcatta cgctcgacca ccatatccat
    17941 agttcctaga tgactctctt tctgagaagt gatttcctta gccagcaaaa ttaagcccgc
    18001 acaggtccca aacactggta agccagatag aatggcttct cggatgggaa gtagcatgtt
    18061 ctggtcacgt aagagcttgc ccatggttgt agactcacca ccaggcaaaa tcaaacccga
    18121 caagtcactc tgatcttgct gaaaatcatc tagatttctg agttctacac tctcgacacc
    18181 taattgatct agcacttttg catgttctgc aaaggcccct tgcaaggcca atattccgat
    18241 tttcatctat tttcctcgtt cagccatgag aatttggatt tcattttcat taataccaac
    18301 catggcttct cctaaatctt cagagatttg agctaggatt tgaggattac ggaagttagt
    18361 cacagcctta acaatggcac tcgctcgttt aacaggatct cctgacttga aaatacctga
    18421 accgacaaag accccctctg cccctaattg catcattaac gcagcatctg ctggcgttgc
    18481 aacacctcca gcagcgaaat ttacaactgg caattttcca tgttcatgaa catattggac
    18541 caattctaca gggacttgca aatccttggc agcaacataa agctcgtcct cacgtaagtt
    18601 ttgaatgcgg cgaatttcct gattcatcat acgcatatga cgaacagctt ggacgatatc
    18661 ccctgtccct ggttctcctt tggtacgaat catggaagca ccttcagcga tacgacgcaa
    18721 ggcttcaccc aaatccttag caccacagac aaaaggaact tggaattctt tcttgtccac
    18781 atggaaacgg tcatcagctg gagatagaac ttcactctcg tcgatataat caatttcaat
    18841 agcctctaaa atctgagctt caacaaaatg cccgattctg accttagcca ttactggaat
    18901 actaaccgct tcttggattt ccttaatcat ctttgggtcg ctcatgcggg aaactcctcc
    18961 agctgcacga atatcagccg gaattcgttc caaggccatc acagctgccg caccagcagc
    19021 ttctgcgata cgagcctgtt caggattctg cacatccata ataacaccac ccttgagcat
    19081 ctgtgccaag tttttattta gttcataacg attttcagtc atttgtttta tcctcatcat
    19141 ttgtattggt agctaccttt tcattttaag tcagaacaga atgaagcaca agataaaaaa
    19201 actataattg tatggggaca gattggctaa aaattatctg accttaactt aaggccagat
    19261 agctcattca tttctatttt tcagccgtaa gggcagccat tgtgatgtag ttgtatggtt
    19321 tgttgaagtg tggcaagaag aagaggtctg tcaatgccaa tttatcaatt gtcacatgct
    19381 cttggatagc aagtgagaac atgtggattc ccatgctaat tgcaatatca tgtgaaacca
    19441 tttgggcacc aagaatttca cggctatctt tgtcaaagac aatcttaatt gctacttcat
    19501 ggttgtcatg tttcatgaat tctggttttt gaagatcgtt aaagcctgtt tcagttgcgt
    19561 tgtaaccagc agctttcgct ttttcaagag tcaaaccagt tgaaaccatg tgaagaccgt
    19621 agattgagat accatttgaa ccttgaacac cgattccttc caattcatgt ccacaagcat
    19681 tgtaggcacc aacgatacca gtgcgaacag catttgaagc aagagcgata tagcttgtat
    19741 ctttacgagc attgtcataa acagtcgcac agtcaccaac agcgtaaacg cctgggatag
    19801 atgtttcttg tttcttgtct acaaggaagg caccgttgcg gaagagttcg atcttaccat
    19861 ctgcaagggc tgtgtttgga cggaaaccaa ctgcaaggat aaccatatcc acgtcaaagc
    19921 tttctttgtc agtaatcaag cgttcaactt taccgtcacc ttcgattgct ttaacagttt
    19981 gacctagagc caagcggatg ttgtgatctt ccaagttctt cgccatcatt tgtgtgaagt
    20041 ctttgtcata gtaaccgttc aagacagtat caacgatatc aacaaggaca acttcttttc
    20101 caagacgttc aaaggcttca gcaagttcaa caccgatgta accaccacca acaacggcga
    20161 tacggtcgag gtgttggctc ttgtcagaaa gtttattgat aacttcttca gcattttggt
    20221 acaatttcac gaattgtacg ttttcaagag ttgctttaaa ttcgcggttt cctttaacaa
    20281 tttcaacacc ttcgattggt ggcaagattg gtgtagaacc tgtagcgaaa atcaattttt
    20341 cgtatgattc tttgtgctct tttccttcaa cttccgctgt aactactttg ttatcatagt
    20401 cgattgaaag aacaggtgag ttcatgtaaa ctttagcacc tttagcttcc aatttttctt
    20461 tatcagaata gaacaagcct tcagcaccgt caatttgttc accaatccaa agagccattc
    20521 cacatcctag gaaagagatg ttagagtttt ggtcaaatac aacaatttcg ttctcatttc
    20581 caaaattatc caacatggta ttgatacatg ctgtaccagc gtggttagca ccgactacaa
    20641 cgattttact cataaaaatt tcctgccttc attctaaatt taccttacta gtataccatt
    20701 taccgttagc gtttacaagg ggggtactat tatttcagca ttttttatca aaaataattc
    20761 cccatttatc ctgttttcaa gcctgatgaa ttttatgagc atatttcttg actttttcca
    20821 aattttgttt atgaaaattc taactttatg gtattataat aacatgaaaa tgaaatgtaa
    20881 gggcttcaat ttagatgatt atttgatatt acaacttatt ttctataaga gaaaggagtt
    20941 ggtagcttga ctcttcgttc acattctgaa cggctaatgg ggactactat cactatttca
    21001 ttagtggatg agcaagccga tatctttctc caaaaatcct ttgacttgct caaagaactt
    21061 gaataccgct tcaacgccaa tagtcaagaa tctgagttga tggaaatcaa ttatcaagca
    21121 ggaatagccc ccgtcacggt tcatccagac ctgtttgagc tgatttcact tgggttagag
    21181 catagcctag cgctctctag ccacctcaat atcagcattg gtcccttaat tcaaacctgg
    21241 cgtatcggtt tttcagatgc caaggtcgcg cagcctcaag aaattgaatc ggtgctgcct
    21301 ctaatcaatc ctcatggtat cgagttagat tcttctactt ctactgtgtt tttaaaacag
    21361 aaaggaatga agatcgatct aggttgttta gccaaaggat acagtgcgga taaggttgcc
    21421 caatttctta ggaaagaggg ggtgacttct gccttgatca atctgggagg gaatatcctg
    21481 accattggaa aaaatcaggc aagaggggat aacccatggc aaatcgggat tcaagaccca
    21541 gccaatccta ggggaaatca tttaatgacc atccctgttg tcaataaatc tgtcgtgact
    21601 tcaggcattt atgaacgtca cctgaccgtc gatggacaag attaccatca catttttgac
    21661 agtcaaacag gatatcctgt tgaaacggaa ctagcgagtc taacgatcat ctctgataaa
    21721 tcagtcgatg gcgaaatctg gacgactcga ctctttggag aaagacctgc ttctatcctc
    21781 tggcaagtcg aaagtttgga gggcatcgaa gtcatcctca tcgataaaga aggccaccta
    21841 agctgttctt caggaattcc tactctatag agacaactgt ttcaatggaa ttttaaaatt
    21901 gtattatgta aaaagagaaa ggaaatctca tgttaaaact tattgctatt gttggaacaa
    21961 attcaaaacg ttctacaaac cgtcaattgc ttcaatacat gcaaaaacac tttgctgaca
    22021 aagctgaaat tgaacttgtt gaaatcaaga ccattcctgt cttcaacaaa ccagctgaca
    22081 agcaagtacc tgctgaaata ttggaaattg ctgctaaaat cgaagaggca gatggcgtta
    22141 ttatcggtac tcctgagtat gatcactcta ttccagctgt tttgatgagc gctcttgctt
    22201 ggttgtctta tggtatttac ccacttttga acaaaccaat catgattaca ggtgcttctt
    22261 acggtacgct tggttcatct cgtgcccaat tgcaacttcg tcaaatcttg aatgctcctg
    22321 aaatcaaggc aaatgttctt ccagatgaat tcttgctctc acactctctt caagcattta
    22381 acccaagtgg cgacttggtt gaccttgatg ttatcaagaa attggatgcc atctttgatg
    22441 acttccgtat cttcgtgaag attactgaga aattgcgcaa tgcgcaagaa ttgcttcgca
    22501 aagatgctga agactttgac tgggaaaatt tgtaagatag gagaccgaaa gaatgaaatt
    22561 tgttggactt gttggatcaa actacgatca atcatataac cgcaaactct tggaatttat
    22621 ccgtcgcaat ttcaaattca aatttgaatt agaagtactt gaaatcgacg aagttccaat
    22681 gtttaaccaa gatgaaaaat gggacgaaag cttccaattg cgtttcttgt ataacaagat
    22741 tacacgtgct gatggtgtca ttatcgccac tcctgagcac aaccacacta tctcagcttc
    22801 actcaaatct gtacttgaat ggctttcata cgaagttcat ccatttgaaa acaagcctgt
    22861 tatgattgtg ggtgcatcat actatgacca aggaacatca cgtgctcaag ttcaccttcg
    22921 taaaatcctt gatgctccag gtgttaatgc ctacacgctt ccaggtaacg aattccttct
    22981 tggtaaagct aaggaagctt ttgataacaa cggaaatatc gccaacgaag gcactgttaa
    23041 attccttgaa acttgcttag ataactttgt taaatacgta ggagtcgttt cgaaattgaa
    23101 aaaaccaaaa ccaatcgaat cagaagactt ggattgtgga aaaccaatcg ctacaaccat
    23161 cacagaagtt gaccctgacg atccagaatg ggtagaaaaa gttgcagcaa tcactggtgc
    23221 tgtttctggt gatacctatg tcaaattgga ccacggtatc ctaacagtta accaaattga
    23281 tatgttcttg aaagctatgc catttgaatt gacatacgct gacgacaaca accaattcct
    23341 ctactacaac aacgctcacc aagatccaga cactatgttc gctaaacgtg taccacctca
    23401 atcaggtagc cgtatgtcga ctgttcatgg ttctcttcca ccagcacgta tgaagaacgt
    23461 agagtgggtt atcggaacac ttcgcaacgg aaaccaagaa tacgtccgta cgatcgttcc
    23521 aggttctcct gcaggtgtta tcaacactca caactaccaa gcaatgtact atcctgatgg
    23581 atcatacgct ggtatcaatg aaatcgtctt taacttccaa ccatggcttg actggtacct
    23641 aaaagagact ggtcaacgtt tggtgggtgg tagcggacca tttgctcctg ctgccggagg
    23701 tcatggagac gccgatgcta cttctggcgc ttctgattca ggggatgctg gaggccacgg
    23761 tggtgacgca gacgctacag ccggtgcaag ttactaatga tagtttttag gaaccattat
    23821 ggttcctttt ttagcgaaaa aagactagca aacacaaact gctagtctct ttgttaaaag
    23881 aaagagtata tattatgaat ttgggtcatc aagactacgg ccatgtaatc ctttttcacg
    23941 ttgtacttgg cgtagttttt ctggtgtaac atcgtttcct tcttcgtcca caattttgat
    24001 tccttcaatg tggtgacgaa cagcgcggcg ataaccttcg atgtactcct cacgtagttt
    24061 ggcttgttcc actttttctt ctggtgttaa gccttctgtt tttttctttt tagcaagctc
    24121 attgatacga gcaatttttt tcggatccat ttcttctcct ttgtaataag ataaagtccg
    24181 tttatcaaat tttatttagt gttaatttgg ttaaaacaag caaacttagc tgctttcttg
    24241 gttagttgtg acaagattgc caaacgattt tgacggacag cctgatcttc agccattacc
    24301 atagtatttt caaagaaagc atcaatgact gggctaagcg caaagagttg tttcaattgc
    24361 tgacttgcag gtcctgataa aacgagtgtt tctactgctt ctgccaaagc tttttcttgg
    24421 tcattctcaa atagttctga atcaactgta gcaacccctt ctgccttctc agccaggtta
    24481 aaggcacgag aaagtgattc aacagatggt ttaaaatctt cttccttgct tacttctacg
    24541 agagcacttg ctgcttccaa catatctgcc acaacaaagt ttgaacctgc aagaactgct
    24601 tccttgatat cttttggagt agagcccatc atcttatcaa cacgagcctt gataaagtcc
    24661 ataacctctg ctttattttc ataagtcaaa ctgtcaaatt tcaatgcata aaggctatca
    24721 atcagctcat ccatagcaat gtgccaacca aaggcatcca agatacgaac cacaccttga
    24781 gttgcacgac gaagggcata agggtcatta gaacctgatg gaatcaatcc tactgagaag
    24841 aaactcaaaa tcgtatccaa tttgtctgca atggctagaa ctgcgccgac cttgctctct
    24901 ggaagttctc cttcagctga tgtaggcatg tagtgttcac gaatagcagc tgccaccgct
    24961 ggagtttcac cagcaagaag ggtgtatttt tcacccataa ttccttggag ttcgtcaaat
    25021 tcaccaacca tacctgtcaa caagtcaaac ttgtaaatgg ctgctgcacg agcaaggtca
    25081 actgtttcat ccactgacaa accagctttt tctgccaaaa gtacagtgat ttgacccgta
    25141 cgaatcatgt gttcacgaag ggaaccaatc ttctcatgga aggtgacatt gtttaatttt
    25201 tcaacaagat ctgaaatcac caatttttgg tcttcacgcc agaagaattc tccgtcttcc
    25261 aagcgggcta ccaagacttt ttcatttcct ttgatgacat ttttcaaacg ctctgcgttt
    25321 ccgttacgaa cagaaatgaa gtttggcaag agttttccat cttgatcacg aacaacaaag
    25381 taacgctggt gttccttcat agaagtcacc aagacttctt ctggaacttc aaggtattta
    25441 gcatcaaaac ttcccatgaa ggcagttggg tattcaacca aattcaagac ttcattcagc
    25501 aaatccgcat caatttcgat acgtacacca tgttttgcct caatttcctt gatttggtca
    25561 acaatcattt gctcacgttc acatggatct gcgattacaa actgcttacg aaggtcttct
    25621 tcatagctca atgctgactg aattttggtt tctttcccca agaaacgatg gccacggctc
    25681 acacgacttc ccttgatatc aaggaaatcc aagtcaaact cttgctcatc caagagaaca
    25741 gttaaagtgt ggacaggacg aatgtattca aagctatttc cagcccagtg catgctgaca
    25801 gggaaagtca atgacttcaa gacatctaca acaccaggaa caatggcttc aactgattgg
    25861 ccaacttctt ccttagtgac atagacatat tcttcaccct taatttcacg gaattcgata
    25921 tcttcaacag tcaaaccttt cccacggaca aatccttgag ctgctttggt gaagtttcca
    25981 tcactatcca aggcaatttt ctttgctggt cccttgaaat cttctgtcaa atcagactgt
    26041 ttgtctgata aaccagtcac acgaacagcc aaacgacgtg gtgttgagaa ggtttgaatg
    26101 gcttcaaaag acaggcggtt ttccttgagg aaggctgcca ttttttcgcc tagttgtttt
    26161 tcacttggtg tgacaacata ggctggtaat tcttcaagac cgagttctac taataagttt
    26221 tttgtcatgt tttttctccg aaaagtatct ttttattttc cagtttgcct tatgtgattg
    26281 gcacgcaagc aaccaacttc gttgtttcat tgctaaaatt ggagccaaaa gtctccaatt
    26341 ttgcctagta tccttagttt cactaaggat accttcatca ctgcggcaac tggctcaggg
    26401 ctcactctgt tcgcccattt tcgtaatttg tcactctctt tattctgcgt cttctgctag
    26461 gagtttagct cttgtttctt catccaaaag tgggtagcct aggcgtttgc gttctgcgac
    26521 aaaggttttg gctacgacac gggccaagtt acggatacga gcgatatagc ctgcacgctc
    26581 tgttacagat acggcaccac gcgcgtcaag cagattaaag gtatgtgaac atttgagaac
    26641 atagtcatag gcagggtgta ccaagccttc ttctaatgca cgaccagctt ctttttcaaa
    26701 cttatcaaag ttttcaagca acatttcttg gtccgaaatt tcaaatgaat attttgagtg
    26761 ctcatactca ggctggataa agatttctcc gtattttaca ccatcagccc actcgatatc
    26821 atagacagag tctacttctt gaatgtaaga agccaagcgc tccaaaccat aggtaacttc
    26881 cgcagtcaca gggccagttg ccaatccacc gacttgttgg aaataagtga actgagtgat
    26941 ttccattccg tcaagccaaa cttcccaacc aagaccagct gaaccagttg atgggttttc
    27001 ccagttgtcc tcaacaaaac gaatatcgtg ctccaaagga ttgattccca atttttccaa
    27061 agactcaagg taaagttctt ggatatttga tggagaaggc ttcatgacca cctggaattg
    27121 gtggtgttgg tagagacggt tagggttttc cccataacga ccgtcagcag gacgacgtga
    27181 tggctctaca taagctgcat tccatggctc aggtccgata gcacgaagaa aagtgtaagg
    27241 actcattgtt cccgcacctt tttcattatc ataagcctgc ataagcatac aaccttggtc
    27301 attccaaaat tgttgcaaag taaggatgat ttcctggaaa gtcaatttct tagacattgt
    27361 ttactccttt ttatataaat tacttgcgac atgaggctgc ctcgtcgatc atacgaggca
    27421 agacgagtta gtactgcgtc tagctcaggc actctagtgc tgagcgtggg gcagtccgac
    27481 tgtaaggagg tttctgccac tgatgcagtg aaatgttaat ttctcagaca ttgaatactc
    27541 cttaataaaa aataatttct tgcgacacga gtctgcctcg tcgattatac gaggcaagac
    27601 gagttagtac tgcgtctagc tcagcaccct agtgctgagc gtggagcagt ccgactgtaa
    27661 ggaggtttct gccactgacg cagtgaaatg tcaatttctt agacattgtt tactccttta
    27721 gtttttatat ttctttttca attaatttct gctgaacaaa ccaatctaac aagggaaaag
    27781 ttttttctgt atttgcccat aaatgataag aatctaagac cgcttgtaca ctacctaaat
    27841 tttctaccaa cttatctact gtcaaaaagc tgcgccagta ttcataaaaa atacttgcat
    27901 agttcttatc ataagtcgaa gaaccaaaat cattcaatga atgccaacca tacttcttct
    27961 gaaaaagttc tacgagagat tgattacaaa ttttttccgc ttgaaattcc tcttctgtca
    28021 agaaatactt gcgactaata tattcaacca tcccctcttc gaaccagata taagagtcat
    28081 aaccatcaaa atcatctaaa aaatgctccg accaatgagc taactcatgt cctacaatct
    28141 gtaagaggaa attttcagat aaagaatgat agtgactttc tattgcttga gtttgctgag
    28201 aacactcgta gtccatcaat tgatacaaat acaactcttt ccaaacagct aaatcaggcg
    28261 ccataaccat tcgtctatta tttgtatagg ctggaacagc gctttcccta ataatctgtg
    28321 tagcagcatt aaaatctgac caaataacgg cttgcgataa atcacagact gcaagttcat
    28381 tctttaaaaa agattgataa tctttcaact tttcagtatt tcttactaca aaatcacgaa
    28441 atgtagctag ttgactatcg tcttttacaa gataaaggtt gtccacggct ctcctttctt
    28501 tcacacaata gactgaataa gcaaaagaac cgcgtccgac aatcctaggt tgatttaacc
    28561 taggggcgtc agaaacgcgg ttccacccta atttattact tcattgtttt tgaaaatact
    28621 acagaaagcg ccagctcatt attttgccct acttggctcc cactatccca agctcgcttg
    28681 aagaacgcca taagactttc tttcctgctg actattatat caaaaattag aataatgtac
    28741 aattcttttt atgattttct agaaatccac atcatcaact cgtggagcac ccgattgaac
    28801 agcaatcgtt tttaaggttt ctctctcctc atgactcagt tcaattccaa agcaatcaag
    28861 attagcttga atacgagagg ttgtgacaga ttttggaagt ggtaaaaatc cttctgccaa
    28921 gctccaggcc aaggctatct gagcaatcga ttttccgtga tttgctgcga tttcttgcac
    28981 ttgcttgcta tcaaatagtt ctccctgacc aaaaggcccc caagcttcca ataaaattcc
    29041 cttttcacga cagtaagcta cgacttgatc ttgatacaca cctggcgcca agcgaacttg
    29101 attgaccgca ggaacgatag ttgcagtttc aagcaaggca tccaaatgat ggggaagaaa
    29161 attgctaacg ccgatagcac ggattttccc ttcttgatag aggtcttcca tcgctctcca
    29221 aacttccgca ttgcgagttt tccatgcgtc attttctctg agcggttttg ggttcggcca
    29281 atgaatcaaa tacaaatcca aataatccaa gcccagtttt tctatagatt tttccaaagc
    29341 ttgacgagtt tgctcatagg tttgctgact attccaaagc ttggtagtta cgaacatttc
    29401 ttcacgtgga actccgctat ctttgattgc ttgaccaaca ctttcttcat tctgataaat
    29461 cgccgccgta tcaatatgac gataaccagc cttcaaggct tctaacactg cacgataggc
    29521 ttcttctcca tccttagcct taaaagttcc aaatcccaat actggaattt ctactccatt
    29581 atttaattga taggtattca tatgcttctc cttctatttt attcatcaat tatagcataa
    29641 aacatttttt aagtagtact atcttctgta aaaaataagt ctgtcaaata tctaacagac
    29701 ctattttttc ttgcttattc atcacgacta tagtacagct catgagcttt taggcgagta
    29761 ttgagcatct ctggtatggt cacaaaggta taaccttgat ttttcaaata ctcaatgacc
    29821 cttggcaagg cattgactgt cggactgtga atatcatgca tcaaaacgat agagccgtta
    29881 gctacttgat gctgaatttc tgtcaaaata gatgcttcat ttttactctt ccagtccaga
    29941 ctatccacat cccacatgat aaagctcaaa tccaagctat tgcgaatatc atctgtaata
    30001 gcaccataag gtggacgcat gagtttagaa ctagaaccca gcactttagt tagcacatcc
    30061 tcagtatcag taatctgctt tttagcttca tcaagagaga gttgcgagag aatcggatgg
    30121 ctccaactat ggtttccaac aacatgacct tcagatttta tcctcttcac caagtcctca
    30181 ttcccagaaa catttttccc aagcacaaag aaagtcgctt taatatcata tttagctagg
    30241 gtctctaata cctgcggggt cgttgctgga tttggaccat catcaaaggt tagagcgaca
    30301 actttttgat atttcttatc aaagtaagat tggtacaagg ccgcatcttt ttcgagtaag
    30361 tacgaagatt ggataacatc aaagaaagca gatactggca aggctatctc ttctaaattt
    30421 tcaaccacag gacttggata aaggataatc tgactatcct tgtaatcaaa attccatgca
    30481 gacaagtctt ggtcagagaa gttttttaca atctgctcac tctggtcttg ctctattttt
    30541 ttatcctcta tgaaggaggt caactctttt atcagctgtt ccttagcctt actaacatct
    30601 gaaaacagtt ggtcaagtgt aaaaggttgc ccatcttctg ttaagtgaac ctttcctaga
    30661 ctggtctttt cagtctcttc aaccttaaaa gcaactaaat catagatttg tttggtcaca
    30721 ttacgattaa cgactccctt taaacctgac tcttcttgct ctgtatagta gaaaacaaga
    30781 ttgtccttac tttctagctt gtccttaaca tcttgattta tcaactccct aacagaggaa
    30841 atcactttct ccccttggag aggataatag gcaatcactt cggcttgccc ctgacgaaaa
    30901 tgctccttct gatttccctc actcaattga tcatctttct cttttttgag cgattcaatc
    30961 ttttgctcaa aacttttttg ctggtagatc ttaaaagcaa taaatccgcc taataggcaa
    31021 atagaaattg ctaaaatacc aatcaaagcc aataatacat gtctcgtttt cccgtgtctg
    31081 ccacgtccta gtctactttt attcatattt atatcataac aaatttgagc aataatttca
    31141 atgatacaat ttggcaatcc acaattcctc tagcaagcca aagcatctta catcaactcc
    31201 aacatccgta tctgtagaga ttatgtagtt ttctatctat ggaatttcta gtagattgaa
    31261 gctagaatag tagacctata cttctaaaac attgttagaa atcgatttga ttgtcctgat
    31321 cgatttgtcc tgttcttatt tcattttact ataaaactat gagaagctag gtcatctgag
    31381 aagtaattac tatcgacaat tgcgatagcg tatactggtg caatttgttt gtagctctgg
    31441 tgggtatcac cttcacgttg gcgaattttt tctaggtttt gattaacctg actgcacaga
    31501 taagcccata ggcgattgat gaaaaaattc tgatgatgaa cctgaatttc gatgataact
    31561 tgggttccat tatctaactc cgccaagaca tctatactag tatagaaatc ctgcgctgag
    31621 tagggcatgg aaggcaagac gtgaatgtta cttccctcca aaatcgtcac attttttgct
    31681 ggcaagtcca gcatatcgcg gataaattga caaagcatta gttgggctga tacccggatg
    31741 tctgagaatc ctccttttcc tcctttttct ccttttcctc cttctattat agcacatttt
    31801 taaatctagg tttgctagat tctatgcttc tatctattta ttcggaaaag aaagcttttc
    31861 ttcagaagaa agctaatagc ttatttgaag ttttctcctc tctgttggat agtcccgtct
    31921 atctatgtta taatgaaaga aggatttaaa atcggaaaag gagtatttat gctaaaatta
    31981 ggtgtcatcg gaactggcgc tatcagccat catttcatag aagcagccca taccagtgga
    32041 gaataccaac tggtcgcaat ctattctaga aaactagaaa ctgcagcaac ctttgcttct
    32101 cgctatcaga atatccaact attcgatcaa ttagaagtct tcttcaaatc ttcttttgat
    32161 ttagtctata tcgccagtcc aaactcctta cattttgctc aggcaaaagc tgccttgtct
    32221 gctggtaaac atgttattct tgaaaagcca gctgtcactc aaccacaaga atggtttgat
    32281 ttgattcaaa cagctgaaaa aaataactgt tttatctttg aagcagctcg taattaccac
    32341 gagaaagcct ttactactat caaaaacttt ttagcagata agcaagtgtt aggagcagat
    32401 ttcaactatg ccaaatactc ttccaagatg cctgacttgt tgactggaca aacaccaaat
    32461 gtcttttcag accgttttgc tggtggagcc ctcatggatt tggggattta tcccctctat
    32521 gctgctgttc gtctttttga aaaagctaat gacgctactt accatgctca acagcttgac
    32581 aatagcattg acctaaatgg agatggtatc ctcttctacc cagactatca agttcacatc
    32641 aaggctggaa aaaacatcac ttccaatctt ccttgcgaga tttatacaac agatggaacc
    32701 ttgactctca acacgattga gcatattcgc tcagctattt ttgccgacca ccaaggaaat
    32761 caagtccagc ttcctatcca acaggctcct catacgatga ctgaggaagt cgctgcattt
    32821 gcacacatga tccagcaacc agatctgaat ctctaccaaa cttggctgta tgatgcaggc
    32881 tctgttcatg agctactata taccatgcgc cagactgctg gcattagatt tgaggcagaa
    32941 aaatgaaaac caaactaccg actgaatggc aagaactgag tgaccaactc ggtttccaag
    33001 aattcacccc cattcaaact caactatttg agctccttct tgctggagaa aacctcctag
    33061 gagtgagcca aaccggaact ggtaagaccc tagcgtatct cctaccaagt cttctcagac
    33121 tacaaaagaa aaaagcccaa caactcttga ttctagcacc aaacacggaa cttgctggac
    33181 agatttttga tgtatgtaaa acgtgggcag aagctatcgg attgactgct caactcttcc
    33241 tttcaggttc aagtcagaaa cgacaaattg aacgtcttaa aaaggggcca gaaattctga
    33301 ttggaactcc tggccgtatc tttgaactca ttaaacttaa aaaaatcaag atgatgaatg
    33361 tggaaaccat catcctggat gaatttgacc aattactaga tgattctcag attcactttg
    33421 tagagaaaat cactcactac gcacctcgtg accaccaact ggtctacatg agtgccacga
    33481 ccaagtttga ccaagaaaag attgtgccga atacacgcac tattgatctc tctgatcaaa
    33541 aattggacaa tatccagcat ttctacatgc aggtagacca acgtcatcga gtggatatgc
    33601 tacgaaaact ggctcatgta gaggatttcc gtggtctagt cttcttcaat agcctgtcag
    33661 accttgggaa tgccgaggaa aaactacagt atcgtgatat attggctgtt tccctcgcta
    33721 gtgatgttaa tgttaaattt agaaaaatca tcttagaaaa gtttaaagat aaccaactaa
    33781 ccctgcttct tgcaactgac cttttggctc gtgggattga tatcgatagc ctagaatgtg
    33841 tcgtaaactt tgatattcct agagatagcg aaacctacac acaccgtgct ggccgtactg
    33901 gtcgtatggg caaggaaggt tatgttatca ctctcgtcac tcatccagaa gaaatcaaaa
    33961 aacttaaaaa gtttgcaagt atccgagaaa ttgtcctaaa aaaccaagaa ctctatatca
    34021 aataaagccc cattgctcta tacacatcca gtgtatggag cttttttaca agcaattcaa
    34081 ttttatactc atctgttttc aaaaagcatt ctagtccatc tccgattaac gatgggcttt
    34141 atcacctcct tctccagtcc ttgtatgata tcttgaagtt gattcatgac atcttccaaa
    34201 gtttgaaagg ctttattctt aaatccacgt ttacgaatct ctttcaacac ttgttcaatg
    34261 gggttcatct ctggtgtgta tggaggaata aaggtaaaac caatattagt cggaatcttt
    34321 aaggtacttg atttatgcca tatagcattg tccataacga gtaaaaggat aagcttgtga
    34381 aagctcttct aaaaaggcgt tcatccactc agtattacat ctaccagcta ttaagaaaaa
    34441 tgattcgcct gtatgggcat caacagctcc ataacaatag cgaaattctc gtatatagtg
    34501 actatggaca tgtggaccta ctcctattgg agaccaacaa gatcccagtt tactgattct
    34561 accgaaacca gcctcatctt ggtacatcaa gcgaacctta cgaaaacgtc tactatattt
    34621 aaatcgcttt cttgccttct tgaattgaga ttttattttt agacgcgaca atggtttgag
    34681 cgtctgtttt cctaggatgt tctggacgtg gcataatatt tcgccagcca tggcgcttca
    34741 acagttgata gaaggcatca cgtgtgtagg aacgacctaa ctccttttta taagcctgaa
    34801 ataaggcatc aattgtaaca aattctcctg cctctgtagc cttcaaatga cgggcaagaa
    34861 aggctttctc ttcctcaact gtcatatatg catggttacg accaccacgt gtttcttgaa
    34921 ggagagagtc gagtccgaac tcctcatatt ttttacattt tgctaaatc
//
LOCUS       DAVKGU010000015        34503 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKGU010000015 DAVKGU010000000
VERSION     DAVKGU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34503
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.015"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            527..979
                     /locus_tag="VW077_000655"
     CDS             527..979
                     /locus_tag="VW077_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9454719.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGDFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            998..2335
                     /locus_tag="VW077_000656"
     CDS             998..2335
                     /locus_tag="VW077_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9454720.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYFVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2414..3298
                     /locus_tag="VW077_000657"
     CDS             2414..3298
                     /locus_tag="VW077_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9454721.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3298..4131
                     /locus_tag="VW077_000658"
     CDS             3298..4131
                     /locus_tag="VW077_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9454722.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4149..6242
                     /gene="nanB"
                     /locus_tag="VW077_000659"
     CDS             4149..6242
                     /gene="nanB"
                     /locus_tag="VW077_000659"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9454723.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFENATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6254..7357
                     /locus_tag="VW077_000660"
     CDS             6254..7357
                     /locus_tag="VW077_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9454724.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7531..8229
                     /locus_tag="VW077_000661"
     CDS             7531..8229
                     /locus_tag="VW077_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9454725.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8248..9765
                     /locus_tag="VW077_000662"
     CDS             8248..9765
                     /locus_tag="VW077_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9454726.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9890..11218
                     /locus_tag="VW077_000663"
     CDS             9890..11218
                     /locus_tag="VW077_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9454727.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11470..12336
                     /locus_tag="VW077_000664"
     CDS             11470..12336
                     /locus_tag="VW077_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9454728.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAIMIAIVSF
                     VQFKVLGNDVEY"
     gene            12352..13191
                     /locus_tag="VW077_000665"
     CDS             12352..13191
                     /locus_tag="VW077_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9454729.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13463..13915
                     /locus_tag="VW077_000666"
     CDS             13463..13915
                     /locus_tag="VW077_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9454730.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNRFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14012..14356
                     /locus_tag="VW077_000667"
     CDS             14012..14356
                     /locus_tag="VW077_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454731.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVTALIGRYIKLGTY
                     AVKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            14643..15287
                     /locus_tag="VW077_000668"
     CDS             14643..15287
                     /locus_tag="VW077_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9454732.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15456..16373
                     /locus_tag="VW077_000669"
     CDS             15456..16373
                     /locus_tag="VW077_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9454733.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16391..17275
                     /locus_tag="VW077_000670"
     CDS             16391..17275
                     /locus_tag="VW077_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9454734.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKFCMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAKH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17291..18142)
                     /locus_tag="VW077_000671"
     CDS             complement(17291..18142)
                     /locus_tag="VW077_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9454735.1"
                     /translation="MDKPDIATVIDSHFEEMTDLEQEIARYFLQAETIQDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHEAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEVQLERIAQLIEDAERVYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFSSVPNKDSQAYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18336..20393
                     /gene="pbp2b"
                     /locus_tag="VW077_000672"
     CDS             18336..20393
                     /gene="pbp2b"
                     /locus_tag="VW077_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9454736.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKVTTSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKEIA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVPTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIVTDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAESYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQPIVFQGSAPIYSWYKLAYGSFPITAVEALEYSSN
                     AYMVQTALGIMGQTYQPNMFVGTSNLETAMGKLRATFGEYGLGAATGIDLPDESTGFV
                     PKEYSFANYITNSFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20404..21000
                     /gene="recR"
                     /locus_tag="VW077_000673"
     CDS             20404..21000
                     /gene="recR"
                     /locus_tag="VW077_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9454737.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21176..22219
                     /locus_tag="VW077_000674"
     CDS             21176..22219
                     /locus_tag="VW077_000674"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9454738.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22303..23676
                     /locus_tag="VW077_000675"
     CDS             22303..23676
                     /locus_tag="VW077_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9454739.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            23663..24274
                     /locus_tag="VW077_000676"
     CDS             23663..24274
                     /locus_tag="VW077_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9454740.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24365..25069
                     /locus_tag="VW077_000677"
     CDS             24365..25069
                     /locus_tag="VW077_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9454741.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLMTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25288..26661
                     /gene="ftsA"
                     /locus_tag="VW077_000678"
     CDS             25288..26661
                     /gene="ftsA"
                     /locus_tag="VW077_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9454742.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26678..27937
                     /gene="ftsZ"
                     /locus_tag="VW077_000679"
     CDS             26678..27937
                     /gene="ftsZ"
                     /locus_tag="VW077_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9454743.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27942..28613
                     /locus_tag="VW077_000680"
     CDS             27942..28613
                     /locus_tag="VW077_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9454744.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28623..29162
                     /locus_tag="VW077_000681"
     CDS             28623..29162
                     /locus_tag="VW077_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9454745.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29162..29425
                     /locus_tag="VW077_000682"
     CDS             29162..29425
                     /locus_tag="VW077_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9454746.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29422..30207
                     /locus_tag="VW077_000683"
     CDS             29422..30207
                     /locus_tag="VW077_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9454747.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30216..31004
                     /gene="divIVA"
                     /locus_tag="VW077_000684"
     CDS             30216..31004
                     /gene="divIVA"
                     /locus_tag="VW077_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9454748.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31352..34144
                     /locus_tag="VW077_000685"
     CDS             31352..34144
                     /locus_tag="VW077_000685"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9454749.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
BASE COUNT        10616 a         6090 c         7514 g        10283 t
ORIGIN      
        1 atttctctct taaaagtttt tggtgaaacg agatgtagaa aggagattta gccaaagagt
       61 ctattagtgc tagaataata gattagaatt attttagaaa aacgaagtga gcagcttata
      121 aattcaagtc cccaaataga ttcatactag tatcttttgc aaaaaataaa gggcgacttc
      181 cttcatgaat atcaatttca tctataagga aggtagctaa ttgaactaac ttatttattc
      241 tgtttgtcgc tagaaaaatc agacctcctt gtgaagattg aggagatact taatgaaaat
      301 caaagaagaa actagcaagc tagtagcaga ttgcccaaaa caccgctttg aggttgtaga
      361 taagactgac gtatataatc caaggtgaag cgactgtggt ttgaagagat tttcaaagag
      421 tatagactag agagtagtgt ttttatgtcc ttctagtaga aaatgctaga cagaagaatg
      481 gggaacttgg ataggaaaaa tagattgaga aaggaggtta gaagagatga ttattacaaa
      541 aattagccgt ttaggaactt atgtgggagt aaatccacat tttgcaacat taatagattt
      601 tctagaaaaa acaggactag aaaatttaac agaaggttcg attgctatcg atggtaatcg
      661 attgtttggg aattgcttta cttatctagc agatggtcaa gcaggggatt tctttgaaac
      721 acaccaaaaa tatttggata ttcatttagt tttggaaaac gaagaagcca tggctgttac
      781 atcgccggaa aatgtaagcg ttacccaaga atatgatgaa gagaaagata ttgaattata
      841 cacagggaaa gtggaacagt tggttcattt gagagctggt gaatgcctca tcacttttcc
      901 agaagattta catcaaccca aggttcgtat aaatgatgaa cctgtgaaaa aagttgtctt
      961 taaagttgcg atttcttaat gtagaaagag aagaacgatg aaaaaaatga gaaagttttt
     1021 atgtctagct ggaattgcgc tagcggctgt tgccttggta gcttgttcag gaaaaaaaga
     1081 agctacaact agtactgaac caccaacaga attatctggt gagattacaa tgtggcactc
     1141 ctttactcaa ggaccccgtt tagaaagtat tcaaaaatca gcagatgctt tcatgcaaaa
     1201 gcatccaaaa acgaaaatca agattgaaac attttcttgg aatgacttct atactaaatg
     1261 gactacaggt ttagcaaatg gaaatgtgcc agatatcagt acagctcttc ctaaccaagt
     1321 aatggaaatg gtcaactcag acgctttggt tccgctaaat gattctatca agcgtattgg
     1381 acaagataaa tttaacgaaa ctgccttaaa tgaagcaaaa atcggagatg attactactt
     1441 tgttcctctt tattcacatg cacaagtcat gtgggttaga acagatttgt taaaagaaca
     1501 taatattgag gttcctaaaa cttgggatca actctatgaa gcttctaaaa aattgaaaga
     1561 agctggagtt tatggcttgt ctgttccgtt tggaacaaat gacttaatgg caacacgttt
     1621 cttgaacttc tacgtacgta gtggtggagg aagcctctta acaaaagatc ttaaagcaga
     1681 cttgacaagc caacttgctc aagatggtat taaatactgg gttaaattgt ataaagaaat
     1741 ctcacctcaa gattctttga actttaatgt ccttcaacaa gctaccttgt tctatcaagg
     1801 aaaaacagca tttgacttta actctggctt ccatatcgga ggaattaatg ccaacagtcc
     1861 tcaattgatt gattcgattg atgcttatcc tattccaaaa atcaaagagt ctgataaaga
     1921 tcaaggaatt gaaacctcaa acattccaat ggttgtttgg aaaaattcaa aacatccaga
     1981 agttgctaaa gcattcttag aagcacttta taatgaagaa gactacgtta aattccttga
     2041 ttcaactcca gtaggtatgt tgccaactat taaggggatt agcgattctg cagcctataa
     2101 agaaaatgaa actcgtaaga aatttaaaca tgctgaagaa gtaattactg aagctgttaa
     2161 aaaaggtact gctattggtt atgaaaatgg accaagtgta caagctggta tgttaactaa
     2221 ccaacacatt attgaacaaa tgttccaaga tatcattaca aatggaacag atcctatgaa
     2281 agcagcaaaa gaagcagaaa aacaattaaa tgatttattt gaggctgttc agtagatgta
     2341 aaagactaga aaataggtgg gctagtgagc tgaaaagctc tagcccaatc ttgtaaaaga
     2401 agggagaagg agaatggtta aagaacgtaa tttaactcgc tggatatttg ttttgccagc
     2461 tatgattatc gtaggattac tctttgttta tccgtttttc tcgagtattt tttatagctt
     2521 taccaataag catttgatta tgcctaatta taaatttgtt ggtttggcta actataaagc
     2581 tgtgctatca gatcccaact tctttaatgc gttctttaat tcaattaagt ggaccatttt
     2641 ctcattagtt ggtcaagttt tagtagggtt tgtattggct ttagctcttc acagagtacg
     2701 ccacttcaag aaattatata ggaccttatt gattgttcct tgggcatttc ctaccatcgt
     2761 cattgccttt tcttggcagt ggattctaaa cggggtttat ggctacttac ctaatctaat
     2821 cgtaaaatta ggtttaatgg aacatacacc tgcatttttg acagatagta catgggcatt
     2881 cctatgtttg gtgtttatca acatttggtt tggagcacca atgattatgg ttaatgtgct
     2941 ttcagctttg caaacagtac cagaagaaca atttgaggct gctaagatag atggtgcttc
     3001 aagttggcag gtgttcaagt ttatcgtctt tccacatatt aaagtggttg taggacttct
     3061 agttgttttg agaactgtat ggatctttaa taactttgac attatctacc tcattactgg
     3121 tggtggacca gccaatgcta caacgacgct tccaattttt gcttacaacc tgggctgggg
     3181 aactaaattg ttgggtcgtg cttcagcagt tacagtattg ctctttatct tcttggtggc
     3241 gatttgcttt atctactttg ctatcatcag taagtgggaa aaggagggta gaaaataatg
     3301 aagaagaaat ccagtattta tttagatatt ctctcacatg tacttttagt tggtgcgacc
     3361 atcgttgcaa ttttcccatt ggtatggatt atcatatctt ctgtcaaagg gaaaggggaa
     3421 ttaactcagt atccaacacg attttggcct gaacagttta cattagatta tttcactcat
     3481 gttatcaacg atttgcactt cattgataac attcgaaaca gtttaatcat tgccttggct
     3541 acaaccctca ttgcgattat tatttctgct atggcagcct atggtattgt tcgattcttt
     3601 cctaaattgg gagcaatcat gtcgagacta ctcgtcatta cctacatttt cccaccaatt
     3661 ttgttagcaa ttccctattc aattgccatt gctaaagttg ggttaacaaa tagtttattt
     3721 ggcttgatga tggtttatct atcttttagt gttccatatg cagtttggct cttagttgga
     3781 tttttccaaa cagttccaat tggaattgaa gaagcggcta gaattgatgg tgcaaataaa
     3841 tttgttacgt tttataaagt tgtgctaccg attgtagcac caggtattgt agcaacagct
     3901 atttatacat ttatcaatgc ttggaatgaa ttcctgtatg ccttgatttt gattaacaat
     3961 acaggaaaga tgacagtagc agtagccctt cgttcactta atggttcaga aatactagac
     4021 tggggagata tgatggcagc gtctgttatt gtagttcttc catcaattat tttcttctct
     4081 atcatccaaa ataagattgc aagtggatta tcagaaggat ctgtgaagta gacgaaagaa
     4141 ggaaaaaaat gaataaaaga ggtctttatt caaaactagg aatttccgtt gtaggcatta
     4201 gtcttttaat gggagtcccc actttgattc atgcgaatga attaaactat ggtcaactgt
     4261 ccatatctcc tatttttcaa ggaggctcat atcaactgaa caataagagt atagatatca
     4321 gccctttgtt attagataaa ttgtctggag agagtcagac agtagtaatg aaatttaaag
     4381 cagataaacc aaactctctt caagctttgt ttggcctatc taatagtaaa gcaggcttta
     4441 aaaataatta cttttcaatt ttcatgagag attctggtga gataggtgta gaaataagag
     4501 acgcccaaaa gggaataaat tatttatttt ctagaccagc ttcattatgg ggaaagcata
     4561 aaggacaggc agttgaaaat acactagtat ttgtatctga ttctaaagat aaaacataca
     4621 caatgtatgt taatggaata gaagtgttct ctgaaacagt tgatacattt ttgccaattt
     4681 caaatataaa tggtatagat aaggcaacac taggagctgt taatcgtgaa ggcaaggaac
     4741 attacctcgc aaaaggaagt attgatgaaa tcagtctatt taacaaagca attagtgatc
     4801 aggaagtttc aaatattccc ttgtcaaatc catttcagtt aattttccaa tcaggagatt
     4861 ctactcaagc taactatttt agaataccga cactatatac attaagtagt ggaagagttc
     4921 tatcaagtat tgatgcacgt tatggtggga ctcatgattc taaaagtaag attaatattg
     4981 ccacttctta tagtgatgat aatgggaaaa cgtggagtga gccaattttt gctatgaagt
     5041 ttaatgacta tgaggagcag ttagtttact ggccacgaga taataaatta aagaatagtc
     5101 aaattagtgg aagtgcttca ttcatagatt catccattgt tgaagataaa aaatctggga
     5161 aaacgatatt actagctgat gttatgcctg cgggtattgg aaataataat gcaaataaag
     5221 ccgactcagg ttttaaagaa ataaatggcc attattattt aaaactaaag aagaatggag
     5281 ataacgattt ccgttataca gttagagaaa atggtgtcgt ttatgatgaa acaactaata
     5341 aacctacaaa ttatactata aatgataagt atgaagtttt ggagggagga aagtctttaa
     5401 cagtcgaaca atattcggtt gattttgata gtggctcttt aagagaaagg cataatggaa
     5461 aacaggttcc tatgaatgtt ttctacaaag attcgttatt taaagtgact cctactaatt
     5521 atatagcaat gacaactagt cagaatagag gagagagttg ggaacaattt aagttgttgc
     5581 ctccgttctt aggagaaaaa cataatggaa cttacttgtg tcccggacaa ggtttagcat
     5641 taaaatcaag taacagattg atttttgcaa catatactag tggagaacta acctatctca
     5701 tttctgatga tagtggtcaa acatggaaga aatcctcagc ttcaattccg tttgaaaatg
     5761 caacagcaga agcacaaatg gttgaactga gagatggtgt gattagaaca ttctttagaa
     5821 ccactacagg taagatagct tatatgacta gtagagattc tggagaaaca tggtcggaag
     5881 tttcgtatat tgatggaatc caacaaactt catatggcac acaagtatct gcaattaaat
     5941 actctcaatt aattgatgga aaagaagcag tcattttgag tacaccaaat tctagaagtg
     6001 gccgtaaggg aggtcaatta gttgtcggtt tagtcaataa agaagatgat agtattgatt
     6061 ggaaatacca ctatgatatt gatttgcctt cgtatggtta tgcctattct gcgattacag
     6121 aattgccaaa tcatcacata ggtgtactgt ttgaaaaata tgattcgtgg tcgagaaatg
     6181 aattgcattt aagcaatgta gttcagtata tagatttgga aattaatgat ttaacaaaat
     6241 aaaggagaaa aacatggtta aatacggtgt tgttggagca gggtattttg gagctgaatt
     6301 ggctcgctac atgcaaaaaa atgatggagc agagattact cttctctatg atccagataa
     6361 tgcagaggcg attgcagaag aattgggagc aaaagtagca agttccttag atgagttggt
     6421 ttctagcgat gaagtagact gtgttatcgt cgcaactcca aataatcttc ataaggaacc
     6481 ggttattaag gctgcacagc atggtaaaaa tgttttctgt gaaaaaccaa ttgcgctttc
     6541 ttatcaagat tgtcgcgaga tggtagatgc gtgtaaagaa aacaatgtaa cctttatggc
     6601 aggacatatt atgaatttct ttaatggtgt tcatcatgca aaagaactca ttaatcaagg
     6661 agttatcgga gacgttctat attgtcatac agctcgtaat ggttgggaag aacaacaacc
     6721 gtcagtatca tggaaaaaaa ttcgtgaaaa atcaggtggt cacttgtatc accacatcca
     6781 tgaattggat tgcgttcaat tccttatggg gggcatgcct gaaactgtaa ccatgacagg
     6841 tggaaatgtg gcccatgaag gtgaacattt cggtgatgaa gatgatatga tttttgtcaa
     6901 tatggaattt tctaataagc gttttgcctt gttagaatgg ggttcagctt atcgttgggg
     6961 tgaacattat gtcttaatcc aaggaagcaa aggtgccatc cgcttagact tattcaactg
     7021 taaaggaact cttaagctag atgggcaaga aagctatttc ttgattcacg aatcgcaaga
     7081 agaagatgat gatcggactc gtatctatca tagtacagag atggatggag caattgctta
     7141 tggtaaacca ggtaaacgta ctccattatg gctatcatct gtcattgata aagaaatgcg
     7201 ctatctgcat gagattatgg aaggagcccc agtatcagaa gaatttgcaa aacttttgac
     7261 aggtgaagct gccctagaag caattgctac tgcagatgct tgtacccagt ctatgtttga
     7321 agatcgcaaa gtaaaattgt cagaaattgt aaaataaatt ttggtattct cctatttata
     7381 ggtcgacttg ctcctctgaa agtactttta gaggagctgt ttgactttgc tagtttttga
     7441 aactgaaatc tattatacta caaactattg aaagcgtttt aattttaagg tataataatc
     7501 tcatagaaat aaagaaaagg aggaaagagg atgccacaga ttagcaaaga agccttgatt
     7561 gagcaaatca aagatggaat catcgtttct tgtcaggctc ttcctcatga accgctttat
     7621 acagaagcgg gaggggtgat tcccttgctg gtcaaagcgg ctgagcaagg tggagcagtc
     7681 ggtatccgag caaacagtgt tcgcgatatc aaggaaatta aggaagtcac taaacttcca
     7741 atcattggga ttatcaaacg tgattatcca cctcaggaac ccttcatcac ggctactatg
     7801 aaagaagttg atgaattggc agaactggac atcgaggtga ttgctctgga ttgtaccaag
     7861 cgtgaacgct acgatggttt ggaaattcaa gagttcattc gtcaggttaa ggagaaatat
     7921 cctaatcagc ttttgatggc tgatactagt atcttcgaag aagggctagc agctgtagaa
     7981 gcaggaattg actttgtcgg aacaacctta tcaggctaca catcctacag tccaaaagta
     8041 gacggtccag attttgaatt gattaagaaa ctctgtgatg ccggtgtaga tgtcattgca
     8101 gaaggaaaaa ttcatacacc agaacaagcc aaacaaatcc ttgaatatgg agtgcgaggc
     8161 atcgttgttg gtggcgccat tactagacca aaagagatta cagaacgctt cgttgctagt
     8221 cttaaataag atgtgagagg gagttttatg tttaaagttt tacaaaaagt tggaaaagct
     8281 tttatgttac ctatagctat acttcctgca gcaggtctac ttttggggat tggtggtgca
     8341 ctttcaaacc caaccacgat agcaacttat ccaatactag acaatagtat ttttcaatca
     8401 atattccaag taatgagctc tgcaggagag gttgtattca gtaatttgtc actacttctc
     8461 tgtgtgggat tatgtattgg cttagcgaaa cgagataaag gaaccgctgc gttagcagga
     8521 gtaactggtt acttagttat gactgcaacg atcaaagctt tggtaaaact ttttatggca
     8581 gaaggatctg caattgatac tggagttatt ggagcattag ttgtcggaat agttgccgta
     8641 tatttgcaca accgatataa caatattcaa ttaccttccg ctttaggatt ctttggaggt
     8701 tcacgcttcg ttcctattgt tacatcgttc tcttctatct tgattggctt tgtcttcttt
     8761 gttatttggc cacctttcca acaacttctt gtttctacag gtggatatat ttctcaggcg
     8821 ggtccaattg gaacttttct atatggattt ttaatgagac tttctggagc agtaggctta
     8881 catcatataa tttaccctat gttttggtat actgaacttg gtggtgttga aactgttgca
     8941 ggacaaacag tggttggagc tcaaaaaata ttttttgctc aattagccga tccggcccat
     9001 tctggattat ttacagaagg aacaaggttt tttgcaggtc gtttctcaac aatgatgttc
     9061 ggtttaccgg ctgcctgttt agcgatgtac catagtgttc ctaaaaatcg tcgtaaaaaa
     9121 tacgcgggtt tgttttttgg agttgcttta acatctttta ttaccggtat tacagaacca
     9181 attgaattta tgtttctatt cgtcagtccg gttctatatg ttgttcacgc attccttgat
     9241 ggtgttagct tctttattgc agacgtctta aatatttcaa taggaaacac attttcagga
     9301 ggtgtaatcg atttcacttt atttggaatt ttgcagggga acgctaagac gaattgggtt
     9361 cttcagattc caattggact tatttggagt gttttgtatt atattatttt tagatggttc
     9421 attactcaat tcaacgttct aacgccagga cgaggagaag aagtagattc taaagaaatt
     9481 tctgaatccg cagattcaac ttcaaatact gcagattatt taaaacagga tagcctacaa
     9541 attatcagag ccttgggtgg atcaaataat atagaagatg tagatgcttg tgtgacacgt
     9601 ttacgtgtag ctgtaaaaga agttaatcaa gttgataaag cacttttaaa acaaattggt
     9661 gcagttgatg tcttagaagt gaagggtggc attcaagcaa tatatggagc aaaagcaatc
     9721 ttatataaaa atagtattaa tgaaatttta ggtgtagatg attaagtact tactgactta
     9781 ataaaaaaca gaggagagtg atggatgagt aggatgaaat gaaatcgcat acaagaaata
     9841 aagaactcat tatccaagtt ggatacgctt attacatagg agaatacaaa tgaaatttag
     9901 aaaattagct tgtacagtac ttgcgggtgc tgcggttctt ggtcttgctg cttgtggcaa
     9961 ttctggcgga agtaaagatg ctgccaaatc aggtggtgac ggtgccaaaa cagaaatcac
    10021 ttggtgggca ttcccagtat ttacccaaga aaaaactggt gacggtgttg gaacttatga
    10081 aaaatcaatc atcgaagcgt ttgaaaaagc aaacccagat ataaaagtga aattggaaac
    10141 catcgacttc aagtcaggtc ctgaaaaaat cacaacagcc atcgaagcag gaacagctcc
    10201 agacgtactc tttgatgcac caggacgtat catccaatac ggtaaaaacg gtaaattggc
    10261 tgagttgaac gacctcttca cagatgaatt tgttaaagat gtcaacaatg aaaacatcgt
    10321 acaagcaagt aaagctggag acaaggctta tatgtatccg attagttctg ccccattcta
    10381 catggcaatg aacaagaaaa tgttagaaga tgctggagta gcaaaccttg taaaagaagg
    10441 ttggacaact gatgattttg aaaaagtatt gaaagcactt aaagacaagg gttacacacc
    10501 aggttcattg ttcagttctg gtcaaggggg agaccaagga acacgtgcct ttatctctaa
    10561 cctttatagc ggttctgtaa cagatgaaaa agttagcaaa tatacaactg atgatcctaa
    10621 attcgtcaaa ggtcttgaaa aagcaactag ctggattaaa gacaatttga tcaataatgg
    10681 ttcacaattt gacggtgggg cagatatcca aaactttgcc aacggtcaaa catcttacac
    10741 aatcctttgg gcaccagctc aaaatggtat ccaagctaaa cttttagaag caagtaaggt
    10801 agaagtggta gaagtaccat tcccatcaga cgaaggtaag ccagctcttg aataccttgt
    10861 aaacggattt gcagtattca acaataaaga cgacaagaaa gtcgctgcat ctaagaaatt
    10921 catccagttt atcgcagatg acaaagagtg gggacctaaa gacgtagttc gtacaggtgc
    10981 tttcccagtc cgtacttcat ttggaaaact ttatgaagac aaacgcatgg aaacaatcag
    11041 cggctggact caatactact caccatacta caacactatt gatggttttg ctgaaatgag
    11101 aacactttgg ttcccaatgt tgcaatctgt atcaaatggt gacgaaaaac cagcggatgc
    11161 tttgaaagcc ttcactgaaa aagcaaacga aacaatcaaa aaagctatga aacaatagtc
    11221 cttagttatt ctataaaaag tagtttttta aagaacctaa gagtgtatac ccccttttcc
    11281 ctctacacag atagtgtaag aaaagggggc ttttgtttaa aatgtaagaa actgtcacga
    11341 aattaaaatg aagttcttac ataagcgaat cataaaaaat ttcattttga ttttaaaaca
    11401 gttcaagaaa gtcaaaaaat tattctattt gaaagagagg tgccgactgt gaaagtcaat
    11461 aaaatccgta tgcgggaaac agtgatttcc tacgctttcc tagcaccagt attattcttc
    11521 tttgtcatct ttgtgttggc tccgatggtg atgggcttca ttacaagttt ctttaactac
    11581 tcaatgacta aatttgagtt tgtaggcttg gataactata tccgtatgtt taaagatcct
    11641 gtctttacaa aatctctgat taacacagtt attttggtta ttggatctgt accagttgtt
    11701 gttctattct cactctttgt agcatctcag acctatcatc aaaatgtcat tgccagatcc
    11761 ttctaccgtt tcgtcttctt ccttcctgtt gtaacgggta gtgttgccgt gacagttgtt
    11821 tggaaatgga tttatgaccc gctatcaggg attctaaact ttgtccttaa gtcaagccac
    11881 atcatcagcc aaaacatttc ttggttggga gataaaaact gggcattgat ggcgattatg
    11941 attattctct tgaccacttc agttggtcag cccatcatcc tttatatcgc tgccatgggg
    12001 aatattgaca attcactggt tgaagcggcg cgtgttgatg gtgcaactga gtttcaagtt
    12061 ttttggaaga ttaaatggcc aagccttctt ccaacaactc tttatattgc aatcatcaca
    12121 acaattaact cattccagtg ttttgccttg attcagcttt tgacatctgg tggtccaaac
    12181 tactcaacaa gtaccttgat gtactacctt tacgaaaaag ccttccaatt gacagaatac
    12241 ggctatgcca acacaattgg tgtcttcttg gcaatcatga ttgctatcgt aagctttgtt
    12301 caatttaaag tacttggaaa cgacgtagaa tactaaagaa aggagacagc tatgcaatct
    12361 acagaaaaaa aaccattaac agcctttact gttatttcaa caatcatttt gctcttgttg
    12421 actgtgctgt tcatctttcc attctactgg attttgacag gggcattcaa atcacaacct
    12481 gatacaattg ttattcctcc tcagtggttc cctaaaatgc caaccatgga aaacttccaa
    12541 caactcatgg tgcagaaccc tgccttgcaa tggatgtgga actcagtatt tatctcattg
    12601 gtaaccatgt tcttagtctg tgcaacctca tctctagcag gttatgtatt ggctaaaaaa
    12661 cgtttctatg gtcaacgcat tctatttgct atctttatcg ctgctatggc gcttccaaaa
    12721 caagttgtcc ttgtaccatt ggtacgtatc gtcaacttca tgggaatcca cgatactctc
    12781 tgggcagtta tcttgccttt gattggatgg ccattcggtg tcttcctcat gaaacagttc
    12841 agtgaaaata tccctacaga gttgcttgaa tcagctaaaa tcgacggttg tggtgagatt
    12901 cgtaccttct ggagtgtagc cttcccgatt gtgaaaccag ggtttgcagc ccttgcaatc
    12961 tttaccttca tcaatacttg gaatgactac ttcatgcaat tggtaatgtt gacttcacgt
    13021 aacaatttga ccatctcact tggggttgcg accatgcagg ctgaaatggc aaccaactat
    13081 ggtttgatta tggcaggagc tgcccttgct gctgttccaa tcgtcacagt cttcctagtc
    13141 ttccaaaaat ccttcacaca gggtattact atgggagcgg tcaaaggata atactctgag
    13201 aaaatctctt caaactacgt cagcgtcacc ttgccatact taagtattgc ctgcggttag
    13261 cttcctagtt tgttcttcaa ttttcattga gtataggaaa atcaatctat caagatacag
    13321 aagtatattt tatagattta gagaatatag aggttataag tgtctacaaa atggagggta
    13381 tgcagttact ttatgaagtt ttgtcagaca cttataaact taagaatggt tttagttaac
    13441 tatcagaaac gaaggaaaga gtatgatttt tgacgatttg aaaaacatca ccttttacaa
    13501 agggattcat cccaatttag acaaggctat cgactatctc taccaacatc gtaaagattc
    13561 attcgaatta ggaaagtatg agattgatgg agataaagtc tttctagttg ttcaggaaaa
    13621 tgtcctcaat caagttgaga ataatcgatt tgaacaccat aagaactatg cagatttgca
    13681 tttgctgata gaagggcatg aatattcgag ctacggttca cgtatcaaag acgaggcagt
    13741 agcattcgac gaagcgagtg acattggctt tgttcattgt catgaacact acccactctt
    13801 gttgggttat cacaattttg cgattttctt cccaggtgag ccacatcagc caaatggtta
    13861 tgcaggcatg gaagaaaagg ttcgaaaata tctctttaaa attttgattg attaaaaata
    13921 ggatgaattg tttttttgta aagctttgat aatactctac catgaaattg atctttgtga
    13981 ggtagagaaa tgagaataaa atatttaaaa attggtatct tctaagtatg ctgcaagagc
    14041 tagtttctta gatggacagg ggattacagt tgatgagatg gcttggataa ttaggggcat
    14101 tgtgactgca ttgattggta gatacataaa attaggtact tatgcagtta agtatggtat
    14161 tagtatggcg cgctcgatct taagtagggc agctgcaact gcggcagcaa gagtaggatt
    14221 attgactaag atttttggat ggattttacg aatagctgtg ctcgcagctg atgtatatgg
    14281 taattttgcc aacaatattg ctgtagcttg ggatgcacat gataaaatcc ctaacaatgg
    14341 tcgtataaac ttttaaaatg cgagaatgaa agcactttgt atttttttat tgtatatgtt
    14401 agcttggaca gtgcttgcaa tgataatccg tggagggcta gatggatttg atgaacacac
    14461 ttggcgtact attttaattg cttcgctgtc cgggatatat atagattata tgtccataga
    14521 taaaagtaga aaaaagtcca aaagaaaaaa gaaaaaatag atttgttcat ggtagggact
    14581 tatgaaagct ttactgacaa aaaagaaaac agtttacaaa gaaaaatgat ggaggagcaa
    14641 acatggcaca aaaaggagta agccttatca aggcagcatt tgatacagat aactttctca
    14701 tgcgttttag tgagaaagtc ttggacatcg tgacagccaa tcttcttttt gtcgtctctt
    14761 gtttgcccat cgtgacgatt ggagtggcta aaatcagcct ctacgagacc atgttcgaag
    14821 ttaagaagag cagacgggtg cctgttttta aaatctatct aagatctttc aagcaaaatc
    14881 tgaaactagg tcttcagctg ggtttaatgg agttaggaat tgtgtttctt accctttcag
    14941 atctctatct tttctggggt caaacagctc tgcccttcca attgctgaaa gccatttgtc
    15001 taggcattct gattttcctt acgattgtga tgttggctag ctatccaatc gcggcacgtt
    15061 atgatttaac ttggaaagaa gtgttacaaa aagggcttat cttggctagt tttaactttc
    15121 cttggttctt cctcatgtta gccattcttg tcctcattgt gatggttctt tatctgtccg
    15181 ccttcagtct actcttaggt ggctcagtct tcctactttt tgggtttgga ctattggtct
    15241 ttatccagac tggattgatg gagaaaattt tcgcaaaata ccaataggag ccttatttct
    15301 gaaactactt tcaaaggctc caaacgctat tctataagcg agaaactaaa atctaagtgt
    15361 ctataagata ttgaaagcct tttcggtaaa gtgtatacta aagaagtaaa gaaaaatctg
    15421 ctttagcaga aaataaaaat aataggagaa aatctatgtc agatttgaaa aaatacgaag
    15481 gtgtcattcc agccttctac gcatgttatg atgagcaagg agaagtaagt ccagagcgta
    15541 cgcgtgcctt ggttcaatac ttcattgata aaggtgttca aggtctttat gtcaatggtt
    15601 cttctggtga atgtatctac caaagcgttg aagatcgcaa gttgattttg gaagaagtca
    15661 tggcggtagc caaaggtaaa ttgaccatta ttgcccatgt tgcttgcaat aatactaaag
    15721 atagtatgga acttgctcgc catgctgaaa gcttgggagt agatgctatt gcaacgattc
    15781 caccaattta tttccgcttg ccagaatact cagttgccaa atactggaac gatatcagtt
    15841 ctgcagctcc aaacacagac tacgtgattt acaacattcc tcaattggca ggggttgctt
    15901 tgactccaag cctttacaca gaaatgttga aaaatcctcg tgttatcggt gtgaagaact
    15961 cttctatgcc agttcaagat atccaaacct ttgtcagcct tggtggagaa gaccatatcg
    16021 tctttaatgg tcctgatgag cagttcctag gaggacgcct catgggggct agggctggta
    16081 tcggtggtac ttatggtgct atgccagaac tcttcttgaa actcaatcag ttgattgcgg
    16141 ataaggacct agaaacagcg cgtgaattgc agtatgctat caacgcaatc attggtaaac
    16201 tcacttctgc tcatggaaat atgtacggtg tcatcaaaga agtcttgaaa atcaatgaag
    16261 gcttgaatat tggatctgtt cgttcaccat tgacaccagt gactgaagaa gatcgtccag
    16321 ttgtagaagc ggctgctgcc ttgattcgtg aaaccaagga gcgcttcctc taatctaaaa
    16381 ggaggtattt atgacatatt acgttgcaat tgatatcggt ggaaccaaca tcaagtatgg
    16441 tttggttgat caagaggggc aacttcttga atcgcatgaa atgccaactg aggcgcataa
    16501 gggtggacct catatcttac aaaagaccaa agatatcgta gctagttatt tagaaaaagg
    16561 cccagtagca ggtgttgcca tatcttctgc tgggatggtg gatccggata agggtgagat
    16621 tttctatgct gggccgcaaa tccctaacta cgcaggcacc cagttcaaaa aggaaatcga
    16681 agaaagcttt actattcctt gtgagattga aaatgatgtc aactgtgcag gtcttgctga
    16741 ggcagtatct ggttcaggca agggagcaag tgtgacactt tgcttgacca ttggaaccgg
    16801 tatcggtggt tgcttgatta tggataggaa agtcttccat ggttttagca attcagcctg
    16861 tgaagtcggg tatatgcata tgcaggatgg agcttttcaa gacttggctt ctacaacagc
    16921 tttagtgaaa tatgtagctg aagcccatgg agaagatgtt gatcagtgga atggccgtag
    16981 aattttcaaa gaagctacgg aaggcaataa attctgcatg gaaggtattg accgcatggt
    17041 agactatcta ggaaaaggtc tggcaaatat ttgctacgtt gccaatccag aagtggtcat
    17101 tctcggtggc ggtatcatgg gacaagaggc gattctcaaa ccgaaaatcc gcacagcctt
    17161 gaaagaggct ttggtaccaa gtttggctga gaaaacaaga ttagaattcg ccaagcacca
    17221 aaataccgca ggcatgttgg gtgcttatta tcattttaaa acaaaacaat cctagtttgg
    17281 cttagccaaa ctaggatttt ctgacacgtt tttgtctacg atagccgttg agtttcttat
    17341 tttcccaata gctattaaag attttctcct tgctttcacg attgatttcc aaaaagtagg
    17401 cataaatcaa atcgataaag aagagcatag gaagttgagc ggatatacgt tggatgtagg
    17461 aaggctggct gtgggtagct acaagaacgg tctcagtata ggcctggcta tctttattgg
    17521 gaacacttga aaagagtaca gtctttgctc ccatctcctt ggcatccaat agactatcta
    17581 aaatagaagg agttgagcct gaaagtgaga aaccaagtac gagacaattt tcgtccataa
    17641 tgctggttgt ccaggcaaag ccgtcttgat cggtcaaagc ttcgcagacc acacccagac
    17701 gcatgaagcg taatttcatt tcacgagcaa caaggccaga acttcctgtt ccgaagaagt
    17761 agacacgctc agcatcttcg attaattggg caattcgttc tagttggact tcgtcaatca
    17821 agtcttgtgt ttgttccctc atattactat agcttctgag gactcgtttg gtcagtggac
    17881 tgtgcttgga gacttggttg gcttgatttt ctgcctcatg ttggtattgg aaaataaatt
    17941 ctcggtagcc agtaaagcca cactttttag caaagcgggt cagtgcagct tgagagatat
    18001 gtaatttttg agtgacctgt tgggaggaaa ggtcatcttg aatggtttct gcttgtaaaa
    18061 aatagcgagc gatttcttgc tctaggtctg tcatttcttc aaaatgtgaa tcaatgacag
    18121 ttgcgatatc tggtttgtcc atagggaagg ctcctttaaa tgagtcgtgt tggaaaaaga
    18181 ctagattatt gaacttttac attcattata acatataata tagggatgga taaataaaaa
    18241 cgtatcttac tgtaaaaacg aaaaaaagct tctttctttt ccataatttt ctgctcaaat
    18301 tgtggtacaa ttaagagtaa gattttaagt tagaaatgag actgatttgt atgagaaaat
    18361 ttaacagcca ttcgattccg attcggctta atttattgtt ttcaatcgtc attttactct
    18421 ttatgaccat tattggtcgt ttgttgtata tgcaggtttt gaacaaggat ttttatgaaa
    18481 aaaagctagc ctcagctagt cagaccaagg tcacaaccag ttctgctcgt ggggaaatct
    18541 atgatgctag tggaaaacct ttggtagaaa atacgttaaa gcaggttgtt tcctttacgc
    18601 gtagcaataa aatgacggct acagacttaa aagaaatagc taaaaagtta ctgacttatg
    18661 tgagcatcag ttcgccaaat ttgacagaac gccagctggc tgattactat ttggctgatc
    18721 ctgaaatcta taaaaaaaca gtggaagctc tcccaagtga gaaacgcttg gattcagatg
    18781 gcaatcgcct atccgaatca gaactgtata acaatgctgt cgatagtgtc ccaacaagtc
    18841 aactaaacta tacagaggat gaaaagaaag aaatctatct ttttagtcag ttaaatgctg
    18901 ttggaaactt tgcgacagga accattgtga cagatcctct aaatgattct caggtggctg
    18961 ttattgcctc tatttcaaag gagatgcctg gcattagtat ttctacttct tgggatcgaa
    19021 aggttttgga aacttccctt tcttctatag tagggagtgt atccagtgaa aaagctggtc
    19081 tcccagcgga agaagcagaa tcctatctta aaaaaggcta ttctctaaat gaccgtgttg
    19141 gaacctccta tttggaaaag caatatgaag agaccttaca aggaaaacgc tcggtaaaag
    19201 aaatccatct ggataaatat ggcaatatgg aaagcgtgga cacaattgag gaaggtagta
    19261 agggaaacaa tatcaaactg accattgatt tggccttcca agatagcgtg gatgctttgc
    19321 tgaaaagtta tttcaattcc gagctaggaa atggtggagc taaatattct gaaggtgtct
    19381 atgcagtcgc ccttaaccca aaaacaggtg ctgttttatc catgtcaggg atcaaacatg
    19441 acctgaaaac gggagagttg actcctgatt ccttgggaac ggtaaccaat gtctttgtcc
    19501 caggttcggt tgtcaaggct gcgaccatca gctcaggttg ggaaaatggt gttttatcag
    19561 gaaaccaaac cttaacagat cagcctattg ttttccaagg ttcagctcca atttattctt
    19621 ggtataaatt ggcatatgga tcttttccta ttacagctgt ggaagccttg gagtattcat
    19681 ccaatgctta catggttcaa accgctcttg gaatcatggg ccagacctat caaccaaata
    19741 tgtttgttgg aaccagcaat ttggaaacag ctatgggaaa acttcgtgcg acctttggcg
    19801 aatatggctt gggggctgcg accggaattg acctaccaga tgaatctact ggatttgttc
    19861 ccaaagagta tagctttgct aattacatca ccaattcctt tgggcagttt gataactata
    19921 cgcccatgca gttggctcag tatgtagcaa ctattgcaaa taatggtgtt cgtgtggctc
    19981 ctcgtattgt tgaaggcatt tatggtaata atgataaggg aggactgggt gacttgattc
    20041 agcaactgca accgacagag atgaataagg tcaatatatc cgactccgat atgagcatct
    20101 tgcaccaagg tttttatcag gttgcccatg gtactagtgg attgacaact ggacgtgcct
    20161 tttcaaatgg tgccttggta tccattagcg gaaaaacagg tacagccgaa agctatgtgg
    20221 cagatggtca gcaagcaacc aataccaatg cggtggccta tgccccatct gataatcccc
    20281 aaatcgctgt cgcagtggtc tttcctcata ataccaatct aacaaatggt gtaggacctt
    20341 ccattgcgcg tgacattatc aatctgtatc aaaaatacca tccaatgaac tagaaaggaa
    20401 attatgcttt atccaacacc tattgccaag ttgattgaca gttattctaa gttaccaggt
    20461 atcgggatta agacggctac gcgtctggcc ttttatacga ttgggatgtc tgctgatgat
    20521 gtcaatgaat ttgcaaaaaa tctcctttct gctaagagag aattgacata ttgttctatt
    20581 tgtggacgtt tgacagacga cgatccttgt tctatctgta ctgattcgac tcgtgaccag
    20641 acaacaattt tagttcttga ggatagtaga gatgtggcag ccatggaaaa tatccaagaa
    20701 taccatggac tctatcatgt cctgcatggc ctcatttctc ctatgaatgg tatcagtccg
    20761 gacgatatca atcttaagag ccttatgact cgtcttatgg atagtgaggt ttcagaagtg
    20821 attgtggcga ctaatgctac agcggatggt gaagcgacct ccatgtatct ttcacgtttg
    20881 ctcaagccgg ctggtatcaa ggttacgcgt ctagcacgag gtctcgctgt gggagcggac
    20941 attgagtatg cggacgaagt gacactctta cgagcgattg aaaatcggac agagttgtaa
    21001 gtgtaggcaa atttacgaac tccattcatt tataaaaaat caaagaggct gaaaatcgtt
    21061 cctatcggcc tctttttgta tagtgtgatg agtagaatca ggttcaagtt ttaaaaaacc
    21121 aagcaaatat gatatactaa agagcgagta ttctagtaga attaggacaa ataatatgaa
    21181 acaaacgatt attcttttat atggtggacg gagtgcggaa cgcgaagtct ctgtcctttc
    21241 agctgagagt gtcatgcgtg cggtcaatta cgaccgtttc acagtcaaga ctttctttat
    21301 cagtcagtca ggtgactcta tcaaaacgca ggaatttagt catgctccgg ggcaagaaga
    21361 ccgtctcatg accaatgaaa ccattgattg ggataagaaa gttgcaccaa gtgctatcta
    21421 cgaagaaggt gcagtggtct ttccagtcct tcacgggcca atgggagaag atggctctgt
    21481 tcaaggattc ttggaagttt tgaaaatgcc ttacgttggt tgcaacattt tgtcatcaag
    21541 tcttgccatg gataaaatca cgactaagcg tgttctggaa tctgctggta ttgcccaagt
    21601 tccttatgtg gctatcgttg aaggcgatga tgtgactgct aaaatagctg aagtggaaga
    21661 aaaattggct tatccagtct tcactaagcc gtcaaacatg gggtctagtg tcggtatttc
    21721 taagtctgaa aaccaagaag aactccgtca agccttaaaa cttgccttcc gatatgacag
    21781 ccgtgtcttg gttgagcaag gagtgaatgc ccgtgaaatt gaggttggcc tcttgggtaa
    21841 ctacgatgtc aagagcacgc tacctggaga agttgtcaag gacgttgcct tttatgacta
    21901 cgatgccaag tatattgata acaaggttac tatggatatt cctgccaaaa tcagtgatga
    21961 tgtggtggct gtcatgcgtc aaaatgcaga aacagccttc cgtgccattg gtggccttgg
    22021 tctatctcgt tgcgatttct tctatacaga taagggagag atttttctca acgagctcaa
    22081 taccatgcca ggtttcaccc agtggtctat gtacccacta ctttgggaaa atatggggat
    22141 cagctaccca gaactaatcg agcgtttggt tgaccttgcc aaggaaagtt ttgacaagcg
    22201 cgaagcgcat ttgatataaa atgaaagaga gggtagaagc cagaaccatc actgcaaggt
    22261 gactagagtt ctcggacttc agcccttttt aaaggagtag aaatgaaatt aacaatccat
    22321 gaaattgccc aagttgttgg agccaaaaat gatatcagta tctttgagga cactcagtta
    22381 gaaaaagctg agtttgatag tcgtttgatt ggaactggag atttatttgt gccacttaaa
    22441 ggtgcgcgtg atggccatga ctttattgaa acagcctttg aaaatggtgc agcagtaacc
    22501 ttgtctgaga aagaggtctc aaatcatcct tacattttag tagatgatgt tttgacagcc
    22561 tttcaatccc tagcatccta ctatcttgaa aaaacgactg ttgatgtctt tgctgttaca
    22621 ggttcaaatg gcaagacaac gactaaggat atgttggcgc atttactgtc aacaagatac
    22681 aagacctaca aaacacaagg caattacaat aatgagattg gccttcctta cacagttctt
    22741 catatgcctg aaggaacaga aaagttggtt ttggagatgg gacaggatca cttgggagat
    22801 attcatctct tgtctgaatt ggctcgtcca aaaacagcca tcgtgacctt ggttggagaa
    22861 gctcatttgg cctttttcaa agatcgttct gagattgcta aaggaaaaat gcaaattgca
    22921 gacggaatgg cttcaggttc cttgctttta gcgccggctg accctatcgt agaggactat
    22981 ttgccaattg ataaaaaggt ggttcgtttt ggtcaaggag cagagctgga aattactgac
    23041 ttggttgagc gtaaggacag tctgaccttt aaggctaatt tcttggaaca agcccttgat
    23101 ttgccagtaa ctggcaagta caatgcgaca aatgctatga ttgcatccta tgttgccttg
    23161 caagaaggag tttcagagga gcaaattcgt ttggcattcc aacatcttga attgacgcgt
    23221 aaccgtaccg agtggaagaa agcagccaat ggagcagata tcctgtcaga tgtttacaat
    23281 gccaatccaa ctgctatgaa actgatttta gagactttct ctgccattcc agccaatgaa
    23341 ggtggcaaga aaattgcagt gttggcggat atgaaggagc ttggtgacca gtctgttcaa
    23401 cttcataatc agatgatttt gagcctctcc ccagatgtgc ttgatatcgt tattttctat
    23461 ggagaagata ttgctcagtt ggctcaattg gccagtcaaa tgtttccaat cggccacgtt
    23521 tactacttca agaaaacaga agaccaggat caatttgaag acctagtcaa gcaggttaag
    23581 gaaagccttg gagcctatga ccaaatcttg ctaaaaggtt ctaactctat gaatctagcc
    23641 aaattggtag aaagtttaga aaatgaatgc aagtgatttt gctaagtatc ttcaaagaat
    23701 gattgctctt acagatactg gactaacctt tacaaaggat ccgtttgacc gtgagcgcta
    23761 cgaagacttg cgaagtctgt tatctgaaat gttgaatcag gtatcagacc ttgatgcaga
    23821 cgaagtggca gaactcttga aaccgacctc cgcttatgcg actcctttaa tggacgtccg
    23881 tgcttggata gttgaggatg aaaaaatttg tctggttagg ggacaaggag agaatgattg
    23941 ggctttgcca ggtggctttg gcgaggtcgg ttattcacca actgaaaata ttctcaagga
    24001 aattgaagaa gaaaccggtt ttaaagccaa agttgaacga ctactagctg tttttgatac
    24061 caatcgtttc caactacaga gcaaacaata tgcaaagttt gtttttgaat gtaagcttct
    24121 cgatggacaa tttcaagaaa atcaagaaat cgctgacctt caattttttg caattgacca
    24181 actgccgaat ttatctgaaa aacgcattac caaggagcaa atagagcttc tttggcaggt
    24241 ttatcaaggt catagggggc aatatcttga ctaagaagat gattatcgta tttctaaatc
    24301 catttttaac aactagcatg gtataataat atgcaggaaa attttgaatt atgaggaaga
    24361 ctagatgaat ttatgggata ttttctttac gactcaggca accgagccgc ccaaatttga
    24421 ccttttttgg tatgttagcc tatttacgct cttagcctta accttttata cagcccatcg
    24481 ctatcgtgaa aataaggttt accaacgatt tttccaaatc ttgcagactg ttcagttaat
    24541 ccttctttat ggttggtact gggtcaatca tatgccactg tcagaaagcc taccctttta
    24601 ccattgccgt atggctatgt ttgtggtact cttgcttcct ggtcaatcca aatataaaca
    24661 atactttgca ttattgggaa catttgggac attagcagcc tttgtttatc cagtgccaga
    24721 tgcttaccct tttccacata tcaccattct atcctttatc tttggtcatt tagcactctt
    24781 ggggaactct ctagtttatc tattgagaca gtataatgcg cgattgctgg atgtgaaggg
    24841 aatttttctc atgacctttg ccctaaatgc cttgattttt gtggtcaatt tgatgacagg
    24901 tggcgattac ggatttttga caaaaccgcc attggttggg gatcacggtc tagtagctaa
    24961 ttatttactt gtttcaattg tgctggtagc tactatcagt ttgactaaga aaatcttaga
    25021 attcttttta gctcaagaag cagaaaaaat gattgcaaag gaagcttaac acagagcttt
    25081 cttttttgct cttagagagt ttttacaagc agcttataaa ataagaattt ctgaatagac
    25141 aaactcaaaa aatggctggg aaatttagga aaaaagcaag cacgattaaa ttttttgtgt
    25201 tataatattt tgtgaatagc tatgcctatg tttagctatg gaataatacg aagtgcgaaa
    25261 cttggaagat agagaggaag cgatgtaatg gctagagaag gcttttttac aggtctagat
    25321 attggaacaa gctctgtcaa ggtgcttgtg gccgagcaga gaaatggtga attaaatgta
    25381 attggcgtga gtaatgccaa aagtaaaggt gtaaaggatg gaattattgt tgatattgat
    25441 gcagcagcaa ctgctatcaa gtcagccatt tcccaagcgg aagaaaaggc aggcatttcg
    25501 attaaatcag tgaatgtcgg cttgcctggt aatcttttgc aggtagaacc aactcagggg
    25561 atgattccag taacatctga tactaaggaa attacggatc aagatgttga aaatgttgtc
    25621 aaatcagctt tgacaaagag tatgacacct gaccgtgaag tcattacctt tattcctgaa
    25681 gaatttattg tggatggttt ccaagggatt cgtgacccac gtggcatgat gggggttcgc
    25741 cttgaaatgc gtggtttgct ttatacagga cctcgtacta tcttgcacaa tttgcgtaag
    25801 acggttgagc gtgcaggtgt tcaggttgaa aatgttatca tttcaccact agcaatggtt
    25861 cagtctgttt tgaacgaagg ggaacgtgaa tttggtgcta cagtgattga tatgggggca
    25921 ggtcaaacga ctgtcgctac aatccgtaat caagaactcc agttcacaca tattctccaa
    25981 gaaggtggag attatgtaac taaagatatc tccaaggttt tgaaaacctc tcgcaaatta
    26041 gcggaaggct taaaactgaa ttacggggaa gcctatccgc ctcttgcaag caaagaaacc
    26101 ttccaagtag aggttattgg agaagtagaa gcagtcgaag tgacggaagc ctacttgtca
    26161 gaaattattt ctgcacgaat caagcacatc cttgaacaaa tcaagcaaga attagataga
    26221 aggcgtctat tggacctccc tggtggtatt gtcttaatcg gtgggaatgc cattttacca
    26281 ggtatggttg agcttgctca ggaagtcttt ggcgtccgtg tcaagcttta tgttccaaat
    26341 caagttggta tccgtaatcc agcctttgcg catgtgatta gtttatcaga atttgcgggt
    26401 caattaacag aagttaatct tttggctcag ggagcgataa aaggtgagaa tgacttaagt
    26461 catcagccaa ttagttttgg tgggatgctg caaaaaacag ctcagtttgt acaatcaacg
    26521 cctgttcaac cagctcctgc tccagaagta gagccggtgg cgcctacaga accaatggcg
    26581 gatttccaac aagcttcaca aaataaaccg aaattagcag atcgtttccg tggcttgatc
    26641 ggaagcatgt ttgacgaata aagaggaaaa ataaattatg acattttcat ttgatacagc
    26701 tgctgctcaa ggggcagtga ttaaagtaat tggtgtcggt ggaggtggtg gcaatgccat
    26761 caaccgtatg gtcgacgaag gtgttacagg cgtagaattt atcgcagcaa acacagatgt
    26821 acaagcattg agtagtacaa aagctgagac tgttattcag ttgggaccta aattgactcg
    26881 tggtttgggt gcaggaggtc aacctgaggt tggtcgtaaa gccgctgaag aaagcgaaga
    26941 aacactgacg gaagctatta gtggtgccga tatggtcttc atcactgctg gtatgggagg
    27001 aggctctgga actggagctg ctcctgttat tgctcgtatc gccaaagatt taggtgcgct
    27061 tacagttggt gttgtaacac gtccctttgg ttttgaagga agtaagcgtg gacaatttgc
    27121 tgtagaagga atcaatcaac ttcgtgagca tgtagacact ctattgatta tctcaaacaa
    27181 caatttgctt gaaattgttg ataagaaaac accgcttttg gaggctctta gcgaagcgga
    27241 taacgttctt cgtcaaggtg ttcaagggat taccgatttg attaccaatc caggattgat
    27301 taaccttgac tttgccgatg tgaaaacggt aatggcaaac aaagggaatg ctcttatggg
    27361 tattggtatc ggtagtggag aagaacgtgt ggtagaagcg gcacgtaagg caatctattc
    27421 accacttctt gaaacaacta ttgacggtgc tgaggatgtt atcgtcaacg ttactggtgg
    27481 tcttgactta accttgattg aggcagaaga ggcttcacaa attgtgaacc aggcagcagg
    27541 tcaaggagtg aacatctggc tcggtacttc aattgatgaa agtatgcgtg atgaaattcg
    27601 tgtaacagtt gtcgcaacgg gtgttcgtca agaccgcgta gaaaaggttg tggctccaca
    27661 agctagatct gctactaact accgtgagac agtgaaacca gctcattcac atggctttga
    27721 tcgtcatttt gatatggcag aaacagttga attgccaaaa caaaatccac gtcgtttgga
    27781 accaactcag gcatctgctt ttggtgattg ggatcttcgc cgtgaatcga ttgttcgtac
    27841 aacagattca gtcgtttctc cagtcgagcg ctttgaagcc ccaatttcac aagatgaaga
    27901 tgaattggat acacctccat ttttcaaaaa tcgttaagta aatgaatgta aaagaaaata
    27961 cagaacttgt ttttcgagaa gttgcagagg ctagtctgag tgctcatcga gagagtggtt
    28021 cggtctctgt cattgcagtt accaagtatg tagatgtacc gacagcggaa gccttgcttc
    28081 cgctaggtgt tcatcatatc ggtgaaaatc gtgtagataa gtttctggaa aaatatgaag
    28141 ctttaaaaga tcgagatgtg acttggcatt tgattggtac cttgcaaaga cgtaaggtga
    28201 aagatgtcat tcaatacgtt gattatttcc atgcattgga ctcagtaaag ctagcagggg
    28261 aaattcaaaa aagaagtgac cgagtcatca agtgtttcct tcaagtaaat atttctaaag
    28321 aagaaagcaa acacggtttt tcgagagagg aactgctgga aatcttgcca gagttagcca
    28381 gactagataa gattgaatat gttggtttaa tgacgatggc accttttgag gctagcagtg
    28441 agcagttgaa agagattttc aaggcggccc aagatttaca aagagaaatt caagagaaac
    28501 aaattccaaa tatgcctatg accgagttaa gtatgggaat gagtcgtgat tataaagaag
    28561 cgattcaatt cggttccact tttgttcgta taggtacatc attttttaag taggagagaa
    28621 ccatgtcttt aaaagataga ttcgatagat ttatagatta ttttacggag gatgaggatt
    28681 caagtctccc ttatgaaaaa agagatgagc ctgtgtttac tccagtaaat tcttcacagg
    28741 aaccggctct cccaatgaat caaccttcac agtcggctgg tacaaaagag aacaatatca
    28801 ccagacttca tgcaagacaa caggaattgg caaatcagag tcagcgtgca acggataagg
    28861 tcattataga tgttcgttat cctagaaaat atgaggatgc aacagaaatt gttgatttat
    28921 tggcaggaaa cgaaagtatc ttgattgatt ttcagtatat gacagaggtg caggctcgtc
    28981 gttgtttgga ctatttggat ggagcttgtc atgttttagc tggaaatttg aaaaaggtag
    29041 cttctaccat gtatttgttg acaccagtta atgttattgt aaatgttgaa gatatccgtt
    29101 taccagatga agatcaacag ggtgagttcg gttttgatat gaagcgaaat agagtacgat
    29161 aatgattttt ttaattcgta tgatttataa tgcagtggat atttactccc tgattttggt
    29221 agccttcgct gtcatgtctt ggtttccagg tgcctacgaa tccagtttag gtcgttggat
    29281 tgtagcgttg gtgaaaccag tacttgctcc cttgcaacgc ctgcctttac agatagcggg
    29341 tcttgattta tctgtttggg tcgcgattgt tttggttcga tttttaggag aaaacctagt
    29401 gcgttttctg gcgatgatag gatgaataaa gggatttatc agcatttctc catagaagat
    29461 cgtccatttc ttgacaaggg aatggaatgg ataaagaagg tagaagatag ctatgctcct
    29521 tttttaactc cttttatcaa tcctcatcag gagaagctat taaagatttt ggccaaaacc
    29581 tatggtcttg cttgtagcag tagtggggaa ttcgtctcga gtgagtatgt tcgagtttta
    29641 ttatacccag attatttcca accagagttt tcagattttg aaatatctct ccaagaaatt
    29701 gtgtattcca ataaatttga acatttaacg catgctaaga ttttagggac agtcatcaat
    29761 caattaggga ttgaacggaa actttttgga gatatcctag tagatgaaga acgggcgcag
    29821 attatgatta atcagcagtt tcttcttctc tttcaagatg gactaaagaa aattggtcgt
    29881 atacctgttt cgctggagga acgtcctttc accgagaaaa tagataagct agaacagtat
    29941 cgagaactgg atttatctgt gtctagtttt cgattagatg ttcttttatc aaatgttttg
    30001 aaactatcta ggaatcaagc aaaccagttg attgaaaaga aacttgtcca agtaaattat
    30061 catgtggtag acaaatcaga ttacactgtt caagttggag acttgattag tgtgagaaaa
    30121 tttggtcgct tgagattact tcaagataag ggacaaacga aaaaagagaa gaaaaaaata
    30181 accgtccagt tattattaag taagtgagga atagaatgcc aattacatca ttagaaataa
    30241 aggacaagac ttttggaact cgattcagag gttttgatcc agaagaagtc gatgaatttt
    30301 tagatattgt ggttcgtgat tacgaagatc ttgtgcgtgc gaatcatgat aaaaatttgc
    30361 gtattaagag tttagaagag cgtttgtctt actttgatga aataaaagat tcattgagcc
    30421 agtctgtatt gattgctcag gatacagctg agagagtgaa acaggcggcg catgaacgtt
    30481 caaacaatat cattcatcaa gcagagcaag atgcgcaacg cttgttggaa gaagctaaat
    30541 ataaggcaaa cgagattctt cgtcaagcaa ctgataatgc taagaaagtc gctgttgaaa
    30601 cagaagaatt gaagaacaag agccgtgtct tccaccaacg tctcaaatct acaattgaga
    30661 gtcagttggc tattgttgaa tcttcagatt gggaagatat tctccgtcca acagctactt
    30721 atcttcaaac tagtgatgaa gcctttaaag aagtggttag cgaagtactt ggagaaccga
    30781 ttccagctcc aattgaagaa gaaccaattg atatgacacg tcagttctct caagcagaaa
    30841 tggaagaatt acaagctcgt attgaggtag ccgataaaga attgtctgaa tttgaagctc
    30901 agattaaaca ggaagtggaa actccaactc ctgtagtgag tcctcaagtt gaagaagagc
    30961 ctctgctcat ccagttggcc caatgtatga agaaccagaa gtagctccag tgcatccgac
    31021 aggtccaaca ccagctacag aaactgttga ttcagcacca ggatttgaag caccgcaaga
    31081 atccgttaca attttataag aaacgatgtg agaacaatat cttatcctta tatttccagc
    31141 gagcaggaga tggtgtgagt cctgcattcc ctaacgataa gattatcctc tcaaaaactc
    31201 aagtctgaag ctagtaagat ttgacgtttc ccacgttacg ggataagagg gagaaagact
    31261 aaatcttttt ccgaataaag gtggtaccac gattttcgtc ctttttggaa gtcgtggttt
    31321 ttaatttgtt attatttata aaggagatac catgaaactc aaagataccc ttaatcttgg
    31381 gaaaacagct ttcccaatgc gtgcaggcct tccgaccaaa gagccagttt ggcaaaagga
    31441 atgggaagat gcaaaacttt accaacgtcg tcaagaacta aatgaaggga aaccgcattt
    31501 tgtcttgcat gatggccctc catacgcaaa cggaaatatc cacgttggac atgccatgaa
    31561 tcatatctca aaagatatca tcattcgttc taagtctatg tcaggtttta acgcacctta
    31621 tatcccaggt tgggatactc atggtctgcc aatcgagcaa gttttggcaa agcaaggtgt
    31681 gaagcgcaaa gaaatggact tggttgagta cttgaaactc tgtcgtgagt acgctctttc
    31741 tcaagtttat aaacaacgtg atgatttcaa acgcttgggg atgtcaggtg actgggaaaa
    31801 tttatatgtg actttaactc cagactatga agcagcccaa atccgtgtct ttggtgagat
    31861 ggctaataag ggttatatct accgtggtgc caagccagtt tactggtcat ggtcatctga
    31921 gtcagccctt gccgaagcgg aaattgaata ccatgatttg gtttcgactt cactttatta
    31981 tgccaataag gtaaaagatg gcaaaggtat cctagataca gatacttata tcgtagtctg
    32041 gacaacaact ccatttacca tcacagcttc tcgtggtttg actgttggtg cagatattga
    32101 ttacgttttg gttcaacctg ctagtgaaac tcgtaagttt gtcgttgctg cagaattgtt
    32161 gactagcttg tctgagaaat ttggctgggc tgatgttcaa gtgttggcaa cttaccgtgg
    32221 tcaagaactc aaccacatcg taacagaaca cccatgggat acagctgtag atgagttggt
    32281 aatccttggt gatcacgtta ccactgactc tggtacaggt atcgtccata cagcccctgg
    32341 ttttggtgag gacgactaca atgttggtat tgctaatggt cttgaagtcg cagtgactgt
    32401 tgacgaacgc ggtatcatga tggcgaatgc tggtcctgag tttgaaggtc agttctacga
    32461 caaggttgta ccaactgtta ttgaaaaact tggtaacctc cttctagccc aagaagaaat
    32521 ctctcactca tacccatttg actggcgtac taagaaacca atcatctggc gtgcagttcc
    32581 acaatggttt gcctcagttt caaaattccg ccaagaaatc ttggatgcta ttgataaagt
    32641 taaattccat acagaatggg gtaaagtacg tctatataat atgattcgtg accgtggtga
    32701 ctgggttatc tctcgtcaac gtgcttgggg tgttccactt ccaatcttct atgcagaaga
    32761 cggtacagct atcatgacag ctgagactat tgaacatgtg gcgcaactct ttgaagtaca
    32821 tggttcaagc atttggtggg aacgtgatgc caaagacctc ttaccagaag gatttacaca
    32881 tccaggttct ccaaatgggg agttcaagaa agaaacagat attatggatg tatggtttga
    32941 ctcaggttca tcatggaatg gtgttttggt aaatcgtcca aatctgactt atccagctga
    33001 cctttacctt gaaggttctg accaataccg tggttggttc aactcatcac tcattacatc
    33061 tgttgccaac catggcgtag caccttacaa acaaatcttg tcacaaggtt ttacccttga
    33121 tggtaaaggt gagaagatgt ctaaatcact tggaaatacc attgctccaa gtgatgttga
    33181 aaaacaattt ggtgcagaaa tcttgcgtct ctgggtaaca agtgttgact caagcaatga
    33241 cgtgcgtatc tctatggata ttttgagcca agtttctgaa acttaccgta agattcgtaa
    33301 cactcttcgt ttcttgattg ccaatacatc tgactttaac ccagctcaag atgtagtcgc
    33361 ttacgatgaa cttcgttcag ttgataagta catgactatt cgctttaacc agcttgtcaa
    33421 gaccattcgt gatgcctatg cggactttga attcttgaca atctacaagg ccttggtgaa
    33481 ctttatcaac gttgacttgt cagccttcta ccttgatttt gccaaagatg ttgtttacat
    33541 tgaaggtgcc aaatcattgg aacgccgtca aatgcagact gttttctatg acattcttgt
    33601 caaaatcacc aaactcttga caccaatcct tcctcacact gcggaagaaa tctggtcata
    33661 tcttgagttt gaagctgaag acttcgttca attgtcagaa ttgccagaag ctcaaacttt
    33721 tgctaatcaa gaagaagtct tggatacatg ggcagccttc atggacttcc gtggacaagc
    33781 tcaaaaagcc ttggaagaag ctcgtaatgc aaaagttatc gggaaatcgc ttgaagcaca
    33841 cttgacagtt tatccaaatg aagttgtgaa aactctactc gaagcagtaa acagcaatgt
    33901 ggctcaactt ttgatcgtgt cagacttgac catcgcagaa gggccagctc cagaagctgc
    33961 ccttagcttc gaagatgtag ccttcacagt tgaacgcgct gcaggtgaag tatgtgaccg
    34021 ttgccgtcgt attgacccaa caacagcaga acgtagctac caggcagtta tctgtgacca
    34081 ctgtgcaagt atcgtagaag aaaactttgc agatgcagtc gcagaaggat ttgaagagaa
    34141 ataagattga aaagtctagg caaaattcaa tttgagaaga aaagacaact aattttatag
    34201 tctattaaac gcattgtatc acgtttttga acacctgata tgatgcgttt tttattttta
    34261 aaatttgcaa ggtgtgactt tttatactca tactcaatga aaatcaaaaa gcaaactagg
    34321 aagctagccg caggctgctc aaaacactgt tttgaggttg tagataagac tgacgaagtc
    34381 agtaacatat atatggtaag gcgacgctga cgtggtttga agagattttc gaagagtatc
    34441 aacaagaatc aaagagaaac ttagcaagct aacagtagta agataaaata ggaatttgat
    34501 att
//
LOCUS       DAVKGU010000016        32381 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKGU010000016 DAVKGU010000000
VERSION     DAVKGU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.016"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(198..335)
                     /locus_tag="VW077_000686"
                     /pseudo
     CDS             complement(198..335)
                     /locus_tag="VW077_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(713..1402)
                     /gene="rplA"
                     /locus_tag="VW077_000687"
     CDS             complement(713..1402)
                     /gene="rplA"
                     /locus_tag="VW077_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9454750.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1610..2035)
                     /gene="rplK"
                     /locus_tag="VW077_000688"
     CDS             complement(1610..2035)
                     /gene="rplK"
                     /locus_tag="VW077_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9454751.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2167..2304
                     /locus_tag="VW077_000689"
     CDS             2167..2304
                     /locus_tag="VW077_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454752.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(2344..3060)
                     /locus_tag="VW077_000690"
     CDS             complement(2344..3060)
                     /locus_tag="VW077_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9454753.1"
                     /translation="MKKRYLVVTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3063..3533)
                     /locus_tag="VW077_000691"
     CDS             complement(3063..3533)
                     /locus_tag="VW077_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9454754.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLEKETRLRFLEEMSLIQEAVAKAFTAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            complement(3535..4572)
                     /locus_tag="VW077_000692"
     CDS             complement(3535..4572)
                     /locus_tag="VW077_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9454755.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDGQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KEQNIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYIKSALVD"
     gene            complement(4670..5995)
                     /gene="brnQ"
                     /locus_tag="VW077_000693"
     CDS             complement(4670..5995)
                     /gene="brnQ"
                     /locus_tag="VW077_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9454756.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6166..6258)
                     /locus_tag="VW077_000694"
     CDS             complement(6166..6258)
                     /locus_tag="VW077_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9454757.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6301..6453)
                     /locus_tag="VW077_000695"
     CDS             complement(6301..6453)
                     /locus_tag="VW077_000695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9454758.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(6613..7191)
                     /locus_tag="VW077_000696"
     CDS             complement(6613..7191)
                     /locus_tag="VW077_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9454759.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7245..8645)
                     /gene="pepV"
                     /locus_tag="VW077_000697"
     CDS             complement(7245..8645)
                     /gene="pepV"
                     /locus_tag="VW077_000697"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9454760.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8658..9263)
                     /locus_tag="VW077_000698"
     CDS             complement(8658..9263)
                     /locus_tag="VW077_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9454761.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            9434..10234
                     /locus_tag="VW077_000699"
     CDS             9434..10234
                     /locus_tag="VW077_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9454762.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(10416..11249)
                     /locus_tag="VW077_000700"
     CDS             complement(10416..11249)
                     /locus_tag="VW077_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9454763.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDCVLEVLDGQYGLENPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11239..13083)
                     /gene="uvrC"
                     /locus_tag="VW077_000701"
     CDS             complement(11239..13083)
                     /gene="uvrC"
                     /locus_tag="VW077_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9454764.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPSI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(13123..13824)
                     /locus_tag="VW077_000702"
     CDS             complement(13123..13824)
                     /locus_tag="VW077_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9454765.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13903..15135)
                     /gene="murN"
                     /locus_tag="VW077_000703"
     CDS             complement(13903..15135)
                     /gene="murN"
                     /locus_tag="VW077_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9454766.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTIGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEEKLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15140..16360)
                     /gene="murM"
                     /locus_tag="VW077_000704"
     CDS             complement(15140..16360)
                     /gene="murM"
                     /locus_tag="VW077_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9454767.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLKLLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16472..17251)
                     /locus_tag="VW077_000705"
     CDS             complement(16472..17251)
                     /locus_tag="VW077_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9454768.1"
                     /translation="MAVMKIEYYSQVLDMEWGMNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17320..18981)
                     /locus_tag="VW077_000706"
     CDS             complement(17320..18981)
                     /locus_tag="VW077_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9454769.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMSYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19156..21378)
                     /gene="recJ"
                     /locus_tag="VW077_000707"
     CDS             complement(19156..21378)
                     /gene="recJ"
                     /locus_tag="VW077_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9454770.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21654..22412
                     /locus_tag="VW077_000708"
     CDS             21654..22412
                     /locus_tag="VW077_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9454771.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LSPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEARAQQFLSKIINHESDKVK"
     gene            22452..23216
                     /locus_tag="VW077_000709"
     CDS             22452..23216
                     /locus_tag="VW077_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9454772.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDTLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23229..23618
                     /locus_tag="VW077_000710"
     CDS             23229..23618
                     /locus_tag="VW077_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131131.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU9454773.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGVFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSLHPDCPHLLQL"
     gene            23823..25013
                     /locus_tag="VW077_000711"
     CDS             23823..25013
                     /locus_tag="VW077_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9454774.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKRVHFYYSFRGEENAVYLDLLRDYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(25334..26215)
                     /locus_tag="VW077_000712"
     CDS             complement(25334..26215)
                     /locus_tag="VW077_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9454775.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(26356..27684)
                     /gene="vncS"
                     /locus_tag="VW077_000713"
     CDS             complement(26356..27684)
                     /gene="vncS"
                     /locus_tag="VW077_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9454776.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVAQD"
     gene            complement(27681..28337)
                     /gene="vncR"
                     /locus_tag="VW077_000714"
     CDS             complement(27681..28337)
                     /gene="vncR"
                     /locus_tag="VW077_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9454777.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(28386..28469)
                     /gene="pep27"
                     /locus_tag="VW077_000715"
     CDS             complement(28386..28469)
                     /gene="pep27"
                     /locus_tag="VW077_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9454778.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(28649..30028)
                     /gene="vex3"
                     /locus_tag="VW077_000716"
     CDS             complement(28649..30028)
                     /gene="vex3"
                     /locus_tag="VW077_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9454779.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPEIKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30080..30727)
                     /gene="vex2"
                     /locus_tag="VW077_000717"
     CDS             complement(30080..30727)
                     /gene="vex2"
                     /locus_tag="VW077_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9454780.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(30740..32017)
                     /locus_tag="VW077_000718"
     CDS             complement(30740..32017)
                     /locus_tag="VW077_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454781.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9192 a         7380 c         6062 g         9747 t
ORIGIN      
        1 agtcagtaac catacgcacg gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga
       61 gtattagaaa gctcgatgga ctattccaga ttcattccac tagatttcga aatcgagtag
      121 gggataatct ctagcccctc tcacaccacc gtacgtgccg ttcggcatac ggcggttcaa
      181 ctaactgtta acgcatgtca ttcaaggtaa taatccaaac acgaaaccag tccacgtttt
      241 tcaaggactg gttttgatat agcacgttta agtaccgact tctgagctac taattgatag
      301 tggtcgcccc agccagatac cttatccgct atataggcct tattatcacg ttcctgttcg
      361 tagagccacg atttcgctat cccttcctct gacgattctt atttccagcg tcagacttaa
      421 aacggtctat ccccagaccg ttttaatccg ctacctcacg atagtcagac ttgggaatcg
      481 ctattgggtt caccggtagc aggtgcccac cgaggactta cacctcaaat atataataaa
      541 atgaaataag aataggacga atcgatcagg acagtcaaat caatttctaa caatgtttta
      601 gaagtagatg tgtactattc tagtttcaat ctactataca acatgcccgt cgtacatcaa
      661 aaaaagttga gacatagcgt cttcaacttt tttacattac tttaactact gattaaagtg
      721 agtttacgtc aactttgata ccaacacctt gagtagttgt gatagtcaag tttgttacgt
      781 aagttccttt agctgtagct ggttttgctt tttggattgt ttcgttgaaa gctttaaagt
      841 tttcaaccaa tttttcagct tcaaatgata ctttaccgat gattgcttga acgttacctg
      901 cacggtcagc acggtaagtg attttaccac ctttagactc ttcaactgct ttagcaacat
      961 ccattgttac agtaccagtt ttagggtttg gcatcaagtt acgtggtcca aggacacgtc
     1021 caagacgtcc aacaagagcc atcatatcag gtgtagcgat aactacgtcg aagtccaacc
     1081 aaccgtcgtt gattttagca acaaggtcat cttcaccaac aaagtctgca ccagcagctt
     1141 ttgcttcttc agcttttgca ccacgtgcga aaacaagaac acgtgaagtt ttaccagtac
     1201 cgtttggcaa taccattgct ccacggattt gttggtcagc ttttttaacg tcgatgttca
     1261 agttgtaagc aacttctaca gttgcatcaa attttgcaaa gttagtttct tttgcaagtg
     1321 ctacagcttc ttctacactg tatgcttttg tgctgtcgat tttctcaaga gcagcacgaa
     1381 gttgtttgct ttttttagcc attttctatt ctccttgtaa gtggttcaat cgattttcat
     1441 ctcccacgtc acttctcgaa atgatgaagt cttgcgggtt ttcagtctac tatttttctc
     1501 ttcctacggc cttaagctcc cttgctgtac ccaagtacaa gcctcagtca cttgcctagt
     1561 atgaaaagca actaatagac tgctgtgaga ttttctactg tgttattgat tagtcaacaa
     1621 cagtgaatcc catagaacga gcagtacctt cgatcatacg cattgcagac tctacgtttg
     1681 ctgcgttcaa atctggcatc ttagtttctg caatttcttg tacttgtgca cgagtaactg
     1741 tagcaacttt agttttatta ggtgtacctg atcctttttc aacacctgca gcttttttca
     1801 aaagaacagc agctggtggt gttttcgtaa cgaaagtaaa tgatttatct tcgtaaactg
     1861 agataacaac tggaatgatc ataccagctt ggtcagctgt acgagcgttg aactcttttg
     1921 tgaatcccat gatgttgata ccagcttgac caagagcagg tccaaccggt ggagctggtg
     1981 tagctttacc agcagggatt tgcaatttta caagtttttc gactttttta gccattttta
     2041 aatcctcctt tgtggttttg gcggtaatta gagattttta cctcccacaa gtatgttttt
     2101 cacataccca tccattatac actatttatc tggaattgca agagaaaagt ttatttttta
     2161 tactcaatga aaatcaaaga gcaaactagg aagctagccg caggctgctc aaaatacagc
     2221 tttgaggttg cagataaaac tgacgaagtc agctcaaaac actgttttga ggttgtgaat
     2281 agaaccgacg cagtcagtag gtaaggcgac gctgacgtgg tctgaagaga ttttcgaaga
     2341 gtattaatcg acgtagtctc cgccttcaaa gccatagtat tcttccaaac tgagaccgct
     2401 tgcagcaatt tctttagctt cttttcctac ataacgcagg tgccattcct cagccatata
     2461 gcctgtttcc ttttccttgc ctttgagata acggacaaca aagccataat cagctgcatg
     2521 gtccaagagc cattgggctg ctttttcttc tgtcaccaaa tcaccatcag tcccaatcac
     2581 atcaaaggcc aagcctgtct ggtgttcgct atagccagga cgggcagagt aacggtcagc
     2641 tgctgccttt ccatcttggt tgacataatc ttgatagagc ttggtctgag tttcataact
     2701 tctaaaacca ctgtaatgat cactaatagg gaaacctgcc tcttgcatcg ctttgatgag
     2761 tttgaccaac tcagccttgg ctgttggatt ttccccggga ttatagtctt tcgacaaagg
     2821 ataatgttta ttggctacga tgatttcgtc gtattttcct tggatgctgt agtaatcacc
     2881 tttattgacc acttctgctt ttttctgggc agccccttga gacttactgc cgactgtccc
     2941 atcagctttg gctgtctgtt ctgtcttagc tgtgccatct tcattttttg ctttttcttg
     3001 tgaacaagct gctagactca aggctagcaa ggctgtcacg acaaggtatc ttttcttcat
     3061 ttctactcct ttatttctaa tagtttatct acttctgatg ataaacgttt gactaatctt
     3121 ttgatctcat ccggttttgc ttggcaggtt tctgctgtca tttcttcaaa aggaacccac
     3181 caaactggac cacgtccctt gagaccgtga ccattcatat cacctgtccg tcgtggaaac
     3241 agatgccaat gaagatgggc atcgccattt cctaaaagtt caatattcat tttctcagca
     3301 gtaaaggcct tagcaactgc ctcttggatt agactcattt cttcgagaaa acgtagtctt
     3361 gtttcctttt ctaaatggtg caattccgtg acgtgttcct tggctagaaa gagactatag
     3421 cctgcaaaat actggtgatc tccaatcaca agatagcctg tttccaactc tttgacaaaa
     3481 tagggatttt ctcccttctt gatgagctca attctctggc atataagaca catattagtc
     3541 taccaacgcg ctcttaatgt aagcatcaac catacgctct gttgcgacca ccgagtcaat
     3601 atgagtacgc tcataagaat gactagactc gatacccgcg ccaaggaggg cgtgtttgac
     3661 ctctgcccct gcggacatgg ccgctgaagc atccgaacca taaaatggat agatgtccag
     3721 cttaaaagga atattttgct ctttcgccaa ggtcaccaag tgttgacgga agtcatagtg
     3781 ataagggcct gaggcatcct tgacacagat agacactgtg tactcatctg tttgctggcc
     3841 atcgcccata gctcccatat caacagccag atattcgact acctgagcag gaatgttaga
     3901 attagcacca tgtcctactt cttcaaaaac tgaaaaagca aaatgagttg ttactggcaa
     3961 ttcaatcttc tctttcttat aaatacgaag caggttgagc aaaatcgctg cgctgacctt
     4021 atcatccaaa tgacgagact tgataaaacc tgtatcagtc acaacagttc gtggatcgaa
     4081 gctgataaaa tcaccgacct caatcccaag agcacgagtt tctttttcat tgcttacttt
     4141 tacatccaaa cgcacttcca tattgtcctg cgtgcgttct gcagttcctg catccttgta
     4201 gacatggcaa gaagtttggt ggatgaggat ggttcctgat accttttgac ctgtgctagc
     4261 cacatgaacg gtacagtttt caccttcaat catgttccaa ggaaaaccac cgatacggtc
     4321 caatttaaga cgaccatctg gtttgacagc acgaacaata gcacccagcg tgtccacatg
     4381 ggcagtcaca tagcgatgtt gctcatcatt ttgacctttg atggtcacat tgacaccacc
     4441 cttggctgtg cgaactggct ggtaaccaaa accttctaga gtcttgacta aataattcga
     4501 aatttcacga gtgaagcccg ttggcgatgc gatggctgtc agttctttga tatattctac
     4561 tgtttgattc atttcttcac ctctttcgct acctattata acacatttat catcggataa
     4621 aaaaagaaaa tcactcctgt agacttggct acaaaagtga tctaagtgat tattccattt
     4681 caaaaacatc gctttcttgc ttgtttggta gaaccaatga gagcaagatt ccaacaatgg
     4741 ctggcaccaa ccatgggaga gatgccttgg caaaaggaag aacgctaaca agatttgcaa
     4801 gaaactcaac cttaaacgag cttcctagta cgcttgcaat ggcaataact gtaaccacag
     4861 caattgtcaa ctgcatacct ggttttgaaa gggcaacaaa tttgttgaca atgacaatca
     4921 taacgatagc aatcgtgatt gggtacaaga taaccagtac tggaattgag tacttgataa
     4981 tcgcatcaag acccaaattg gcaatagcaa atccaatcaa ggtaaaggct gtcgcataaa
     5041 ccttgtagct gatttgtggg aagcgctcat taaagaactc agctgttgac acaatcaaac
     5101 caacagtcgt tgtgaagcag gttacggtaa ccatagctgc aaggaagagt tgagctgttg
     5161 agccaaagat ttcttgagtg gcttgtgaca agatgtaaac acctggtgtt ccacccttca
     5221 tcgcttcagc tggtactggg aaatgatttc caagaaaacc taaaccgatg taaagagcgc
     5281 tgaaggcaag ggcaacaacg ataccaacaa cccaaatagt tgaaatgtat tctttcttac
     5341 ttgaaaatcc aagttgtttc aaggtttgaa ctgcgattac gctaaaggcc actgaggcaa
     5401 gggcgtccaa ggtattgtaa ccttctagga aacctgtacc aaaggcagaa gcttgataag
     5461 cagctgaagc agcttgagga cttgttccac catatttgat agctcccaga acgaccaaga
     5521 taacaatcaa aattgcaaag actggcgtta aaatacgtcc aatgcggtct aagatttttg
     5581 atggattaag cgaaatcaaa taggctgccg caaaatacag aaccgtaaat acaatcaagc
     5641 caagtccttt atttgcatcc gacaaaaggg ggctaatccc tacttcgtaa gctgttgtag
     5701 cagtacgtgg ggtagcaaag aatggaccga ttgacaagta aagaactgag aggtaaagag
     5761 tcgcaaacca aggcgctatc ttcgttgaaa tctcgtagat atatccttta ggatttagcg
     5821 ttccaataat aagggtcaag acggcgatac caacgcctga aaagacaaaa cctgcgatgg
     5881 caggaagaaa atgttctcca gatagagcac ctagagaagg cggaaaaatc aagttccccg
     5941 caccaaaaaa tattccaaac aggagcaaac ctgttagggt acctttttta gccataaaaa
     6001 tctccttcat atttataaaa tactgaccta gtataccacg attaggagtc tgaaaaaagc
     6061 atcacgaggc aaatattaaa tgttttcaat tttcttaaaa ataagaatta tctaaaaata
     6121 aaactctcct acccagtcca caccaagtag gagaaatgca aaattttaaa gttcttcaaa
     6181 ggctaacatg ccaccctgga cattgataac attataacct tgttctaata ggaattggca
     6241 agcacgcgcc gatctcattc cagatttgca aataatataa tgcaagcgat ctttgtccaa
     6301 ttaatcataa ctatcagcca attgactaag cggtaggttg tgggcacctt ctaaatgaag
     6361 agctgcaaac tcatccactt ctctcacatc cactaaagaa agctggtcgt tttggtaaag
     6421 ctggtaaaat gcgtcaaagg ctatttcttt cattcttttt cttctaatac tcttcgaaaa
     6481 tctcttcaaa ccacgtcagc gtcgccttac cgtgcgtatg ttaccgactt cgtcagtttt
     6541 atctgcaact tcaaagctgt actttgagca gcctacgact agcttcctag tttgctcttt
     6601 gattttcatt gactataaaa tggttttaat tctttttttc aaatctggca ctacttctgc
     6661 ctcaaaccaa ggatttttgg ccatccagat ttgatttcgt ggtgatgggt gaactagcgg
     6721 aaaataggct ggcagatagt ctttatagtg tttcaccctc tccgttacct tcccactgat
     6781 tttctcctgt aaatagtagg cttgggcata ttgcccaatc aagagggtta actgaatatc
     6841 aggcaattcc tgtaagagct gcggatgcca tttttctgca aaacctgtac gaggcggaag
     6901 atcacccgac ttgccatgtc ctggaaagta gaaatccata ggcaaaacag caaaataacc
     6961 tgaattgtaa aaggtatctt catccacacc tagccagtcc cgcaagcggt caccactttt
     7021 atctttccag taaaggcctg cttcttgagt tttaagtccc ggagcctgac cgatgatatt
     7081 gatgcgagca gtttttggcg ctgcaaagag aggctcaatg ccacgctctg tatagctggc
     7141 attctgcgaa tccgccatga tagcctgttt gattctttcg atttgagaca tctactttcc
     7201 ctccaaaaag aagtccaagc ataagatctc agacttctat cgttttattt gatcaattcg
     7261 taaatagctt cggcataaat tgctgctgct cggaagagat cgtccaaggc aataaattca
     7321 ttggcttggt gcatggtatc aattgagtct gggaacatag caccataggc aactccgcgc
     7381 tctaacaagc gaccaaaggt tccaccaccg atgacttgtt catgaccttg taggcctgtt
     7441 tgtttttcat agacattcaa caaggtttgc acaagtggat cttccattgg cacatagtga
     7501 ggggtatgac catgttctga tagactgaca gaagcaactg gcaagttttc taggattgat
     7561 ttgatttgtt ctggacttgt tccttttgga tagcggatgt taagggcaat ggtattatca
     7621 gcacttgttt catcgaagcg gaagactccg gcattcatag aaagagcacc catcttttca
     7681 tccacatgag caatcttgag attttcaccc tcatgatcgt tcaagagaat tttacctgcg
     7741 atgtcaaggt agtctttggc tggaccagca aagccaaact ggctgaggaa gagggcaagg
     7801 taagttgcgc cattgacacc tgaagcaggc atagcaccgt gggctgattt accaatgatc
     7861 gtcaccttgt atttgccagc ttcttcttgg agttctcctc taagtttgtg ttctgcaaca
     7921 aaggcatcta gtttagcttg caagtcagcc aaatcacctg aaacgactgc tgttgctgat
     7981 tctggtacca tattttcacg taaaccacct gtaaagctgt gaagacgggc agcacctgta
     8041 ttttctcctg caaagtggag gtattccgtg atatttcctt tttcaccatt gatgattgga
     8101 aattcagcat ctggtgagaa accgaaatct ggtttggcaa gtcctacgtg ctcaaagtag
     8161 tagtccatgt ctgcccagcc tgattcttcg tctgttccaa cgatgaagcg aactttctta
     8221 gaagttggaa gacccaattc tttgatgatt ttcaaaccat agtaacaagc tgttgtagga
     8281 cccttatcgt ccgaagcccc gcgcgcataa aggcgaccat ctttgatagt tggtgtgtaa
     8341 ggatctgtat cccaaccgct accagctgga accacgtcca tatgggcaaa gattccgaga
     8401 acttcttctc catcaccaaa ctcaaaatgt cctgcgtagt tatcgacatt cttagttgga
     8461 taaccatcac ggtctgcgat ttcaaggaat ttttccaagg cttttactgg accaggtcca
     8521 aatggatgtt gggcatcagc cttgctatca tcacgttctg agttgatttc caaaaggcta
     8581 aacaagtcag ccaagaggtc ttctttgcgt ttttctactt ctgctgtaaa atcaattgct
     8641 atcatttttt cttcttccta tcttttttcg atgatttcat ctactggcaa gcggtagctt
     8701 ggttccaatt tgtcgtctgt gtaacccact gtgatcaaga gttctgggcg gaaacggtct
     8761 tcaatttcca aaacctcatt gacttttgat ttgtcaaaac caaggataat attggaacca
     8821 attccttggt ctgtaagagc gagaaccaag ttcatagcaa ccaaacctgc attgagtgct
     8881 aggtagtcgc tgacttgttg ttcattgtag cgtgcaaatt cagctggcaa atttttcatg
     8941 aagtattgaa gttgttcttc tgagaaattg ttagcaccac caactcgggc aatcttacga
     9001 gcgcgtttgg caaggtctgt atctgtaaat agggcaatgg ttacaggtgc tgatgatacc
     9061 tgctcaaagt ttgaaccata agccaacttt gccagttcag catttttctc acgaacaact
     9121 acaaatttcc aaggctggct gttgtgggcg cttggcgcca aggttgcaat ttcgatagcc
     9181 gtacgcacat cttttggatc aacaggctta tcagtgaaat gcttggtcgc atgacgtttt
     9241 ttatttaatt caagaaattt cataatcgat ttccttttct aattctactg cttccattct
     9301 accataattt gaaagagctt gcagggattt ttcatgatta agtttttcta tcacagacat
     9361 aaaagatata tattggttta tcaggaaagt ttctgataaa ctagcaagta ctttacattt
     9421 gaatggagac agtatgaaaa aatttagcct attactagct atcctaccat ttttggttgc
     9481 ctgtgggaat caagctacac ccaaagagac tagcgctcaa aagacaatcg tccttgctac
     9541 agctggcgac gtgccaccat ttgactacga agataaaggc aatctgacag gcttcgatat
     9601 cgaagttcta aaggcagtag atgaaaaact cagcgactac gagattcaat ttcaaagaac
     9661 cgcctgggaa agtatcttcc caggacttga ttctggtcac tatcaggctg cggccaataa
     9721 tttgagttac acaaaagagc gtgctgaaaa atacctttac tcgcttccga tttccaacaa
     9781 tcccctcgtc cttgtcagca acaagaaaaa tcctttgact tctcttgacc agatcgctgg
     9841 taaaacaaca caagaggata ccggaacttc taacgctcaa ttcatcaata actggaatca
     9901 gaaacacact gataatcccg ctacaattaa tttttctggt gaggatattg gtaaacgaat
     9961 cctagacctt gctaacggag agtttgattt cctagttttt gacaaggtat ccgttcaaaa
    10021 gattatcaag gaccgtggtt tagacctctc agtcgttgat ttaccttctg cagatagccc
    10081 cagcaattat atcattttct caagcgacca aaaagagttt aaagagcaat ttgataaagc
    10141 gctcaaagaa ctctatcaag acggaaccct tgaaaaactc agcaatacct atctaggtgg
    10201 ttcttacctc ccagatcaat ctcagttaca ataacactct tcgaaaatct cttcaaacca
    10261 cgtcagcttt atctgcaacc tcaaagctgt gctttgagca acctacggct agcttcctag
    10321 tttgctcttt gattttcatt gagtataaga tatactaaaa gcgattggac cagccaatcg
    10381 cttttttagt ttgcattggc tattttagga aactcttaca aaaaatcaaa aaaattcttc
    10441 acatcctcta catacccatg cttttctatt aagctggcta aaagttccag attgtgcccc
    10501 tgatagttat cgtcacgacg cagttcttca tacatttcaa taaagggaag ccccttggac
    10561 ttggccaatt ctaactcagc ctcgtaatca taagcaactt tacgaaattg gatattgacc
    10621 aattccccat cttcaacttc tatcacggca tactgggcac ggtgattttt taacgcctcc
    10681 caattaaaat agggcatgcc aatcgaccct ggattgatga tttgttgccc ttgacttcca
    10741 taacgaagca actgcttgtg aacatgacca taaactgcca cgtccgtttc cgcatctagc
    10801 agttggtcaa atttctctgt atcattctca actagcaagt caccaccata gttcttattt
    10861 ggcaaattat gagagataga aaagcgcagt ccagcaattt ctttcttttc cagcaaaggc
    10921 aagcttcgta gccagacagt cgttgcagga tccattcgct ccatcaaaaa ctgagtcatt
    10981 cgcatgagct ggatttcttg cggattttcc aaaccgtatt gcccatccaa gacctccagg
    11041 acacaatcat cccaattgcc tcgaacactg gctgtgatag gaaggtcctt tagcagggcg
    11101 actaagtcat ttgcgcctgg accaggaaga aaaatatctc ccagaagcca atattcactg
    11161 gccccttgat ttttagcatc tgcaatcact gcttctagcg ccgtcgcatt gccatgaaca
    11221 tctgataaaa ttgcgatttt atggttcatt gtggtttcct tccgtttggt aatctactct
    11281 ttcttctgct acttgaggca aggcttctcc ctgcgggttc aactttcttt gcacagcctc
    11341 tgctactgct cttggtactc caacttcgac aatctcatcc acactggctt ccttgatttt
    11401 ggtcagagac ttgaagtgcc tcatgagatt ttgcttgcgt ttaggtccca gaccatcaat
    11461 cccatccaat tgagatgaga aagaattttt ggagcgcagt tggcggtgga aggtaatggc
    11521 aaagcggtgc acctcatctt ggatgcgttg gaggaggaaa aattcctgag aattgcgaga
    11581 caagtccacc acctcaagcg gatctccaaa gagcagttca tgggtttggt gcttatcatt
    11641 cttttgcagc ccagcaattg gaatatccaa gcccagttcc tcttggataa cctgcttagc
    11701 gatattgact tgaccttgcc ccccatcaat cacaatcaaa tctggaggag tcaaagcctc
    11761 acgctgtact cgaccatagc gtctgcgaat gacctctctc atgctggcat agtcgtctgg
    11821 tccaacaacc gtttttatct tgtacttgcg gtaatccttc ttactcggtt taccgttgac
    11881 aaagaccacc atagccgaaa cagggctagt tcccatgata ttagagttat cgaaggactc
    11941 gatacgtact ggggtcggga tttggagcaa acgtcctaga ttttcaatag ctccttgggt
    12001 cttctcgaca gatttctcta gcaggttaaa tttctgctcc agactaacac gtgcattttt
    12061 tatggctaga ttgaccagct gttttttctc tccacgttgg ggtttgagaa tcttggtatc
    12121 caccaaagcc ttgacagctt cttcgtcaat atcctgcgga atcagtacct cattgggaac
    12181 taggtgagat ttttcttgat agaattgtcc tacataggtc aaaaaatcct catctggatc
    12241 attgaagtag gggaagagat tgacatcacg ctcgatgagc ttaccctgac ggacaaagaa
    12301 aacctgcaca cacatccagc ccttatccac atagtagcca aagacatcgc gattttggag
    12361 atccttagcc atgacccgtt gcttggttcg aagcgttcca atagcctgaa tcaggtcacg
    12421 gtattccgcc gcacgttcaa actccatagt ctgggctgcc gctgccattt tgccctttag
    12481 ctcatcaatg attttgttat cctgcccttt cagaaaatca gaaacctcct gggccataga
    12541 cttgaaataa gtctcatcct tcttacagat ggtgtgggcc atgcattgac cgatatggta
    12601 ataaaaacag accttagagg gcgggttggt acacttacga aaagggaata tccgatccag
    12661 caaccgcttg atttcattgg ctgcccccac atcgggatag ggtccaaaat aaagacctcc
    12721 gtcctttttg acctgacgag tgataatcaa gcgtggatag cgctcattgg tgattttgat
    12781 ggaaggatag gacttgtcat ccttgagcat gatattgtac ttgggcttgt tttccttgat
    12841 caggttgatt tctaggagaa gtgcctcaat attagactcc gtaacaataa attcaaaatc
    12901 cacaatttca gacaccagag cctctgtctt ggtatcatga cttccacgaa aataggaccg
    12961 tactcgatta cgcagatttt tagcctttcc tacatagata atggtgccat ttttatcctt
    13021 atgaatgtag caaccagggc tggtcggcaa gagctctagt tttgatttaa tcaagttgtt
    13081 catagttcca ttatagcaaa aaaagtaggg aaagatagta ccctactcac tagtctcata
    13141 tatttttcgg aggtttgcaa catcctcttc ctggatacca taaaaggaac ctgctggttg
    13201 catgacagac ttctcatctg tatggtccaa gccaatcgca tgacctaact catgttctgc
    13261 cgtatggaca aggcgttcat aggagtagcc atagtatgga ttggacaaat aataatgatt
    13321 caaccgcacc gttacggaca agaattgccc tgttaagaga ttagtttgac tttccgcctc
    13381 tcctgccaca ggagtgcctc cgtcgttcat ctccgtagcc gtaatatccg ccttactaga
    13441 ctcagtcacg agttcaaagt taaaagcacc agtttgattc cagttctgga ttgcttctaa
    13501 ataagcctct tgaaagcgtg aatccatctg cggatccagg taaatacgag cagaggcctg
    13561 tgcccacctt ccttcagaat gctggtgact atctgtctgg cttaacttag gcagttgccc
    13621 tgttttctcc cattggtcaa tactttgttg accaattttt actgaccgtt ctgcttgctt
    13681 tagcgctcct tgaaaatccc cgttcagata ccagagaagt ccgaaagcaa gcacacataa
    13741 gagcacaact atccaaacca gacgccaaaa caaacgccac acaaaagaaa agaaagcccc
    13801 tatcaaacga aaaagccagc gcatgtctct ccacctttct agcaaataag aattatttta
    13861 ctaaaagaag ctaaaagaaa tctaaatact gactttttca tcttaacgtc ctactatttt
    13921 tttgagtaac tggatagctt tgtattttaa aggcgatgga tggtaacgga aagtacctgc
    13981 tttgcgtaca atatagccat taaaattctg tttaaaacgc aaaacaccat cacttccatc
    14041 aaaaatccct tgaatgccta ggaagttgta tttaggtatt ccacgtttta tgctttccaa
    14101 cataacatat ttttgaagca gtgcaggggc atagaactta ttaaactcag tgtaggaacc
    14161 actaaagaga taagtcgttt cctgaggcat ataaacaaat aaactcccag ctaaaacaat
    14221 atcttcttct ccatattttt caatcaagtc tcgcgcttct gcttttcgaa cttcaaacgt
    14281 ttcaaattga ctagaatatt ctctcagttg attttgtttt ttctcagaat gaggattttt
    14341 actcaaatca agtcgcaact tgtccaattt ttcttctagt ttactttgtt caccttgcaa
    14401 tttgctcata tactccgaaa aatttaagct tgctatgaga aactccgctt gttctccaaa
    14461 agtatcataa aaatgctcat aatattctaa acttttatca ctatattctc tacgttcaga
    14521 ggtttctttt gttatattct taaaaatcga tagttcttca cgttttaact ttttcaaccg
    14581 aatgccaaag gtttcagcct ttttcaccaa gggtttaccc tttttgctaa aacttttaag
    14641 caaactcttt tcagttaatt cagttaaatc tttatagtat aaccaatctg gttctccacc
    14701 tgggtaacct attgttaagc catcaaattg ataacctaaa tcagtcaaac cttgaataat
    14761 actttttttc tcagcatcta ttggattacc ttggctatca aaagtttgat aagtttcata
    14821 aggttttaca agcaactcta atacaccatt ttgcttggca tattctttta actctgcata
    14881 aaaaactgga agagcatctt gttgggtata aatcggcccc gaattgagtt ccatatgcag
    14941 accacccagc atgggcaagc tataaaccag agctgcaact tgaatttctc cttcttgttt
    15001 caaagcaaga taaacaattc gagcccctct tttttctagc aaatccccca tctggacaga
    15061 ttgcataaag gaacgagaag aaacctgatc agaataagtc tgaaactctt ctttcgtgag
    15121 tgttgttagt gccatatact tactttctat gtttttttct taatgtttta cggaaatcaa
    15181 gagcaagtct taacagagga tagagaggat gagtgggcat tgtaaattca cccaagtatt
    15241 cttcaatcgt tggattaaat ttttccttaa aatgataaag tccaccattg agagagtttt
    15301 caacaccacc taaattttgc cagaccatac ctcgctcaaa ggcatagcga gccgtttcat
    15361 accatgttaa aattggtgca ttgtaacgtt taaaatcatc atccatacca gcatatatat
    15421 tgacagaggt agtaccaaat tccaaactca aagtagccgc taaaggaact ctcgcttgac
    15481 ctacatctat atattcctgc aagaaggtca attcctctaa caaacgttct ttttccttct
    15541 tctgcgcttc tacttttgaa gttcgagtcg actcagtaaa cgtctcttcc aaggctctat
    15601 ttttcgctaa ctgttcttct aactcttgcg aacgtttaga aacatccaag gtggccaagg
    15661 tgatataggc cttgtcctta aaattatcta acaatttttt ataataggct tcattcctca
    15721 aatgaatctc ttttcgcttc tcagtttttt tcatcaactc cgaaaatgaa tctaatagtt
    15781 tcagtccacc atattgaatc tcaagccctt tgtttcgtgc tgttcgaata gcctgttttg
    15841 ttgacttgga aagtttatct tcttcaaaat tttccttgta tattttcgcc tgaatacgag
    15901 gttgaatggt gtctcccatt tcctccgttt ttcctgacca ccttactccc atttgttgca
    15961 aactatcaat aatagccaga ttttcaggaa attctgtctt ttcctgattg attaaacttt
    16021 gagataggca aatacttggg tcaaaagtca caaaaaccgc tctcttactg cgagcatagg
    16081 acttaataga ctgaatggca aaattcaaga gttctttatc cccataatcc aatataggtc
    16141 ctcttgggat gtaaaacatt ttatagccta gcggaagagt tctaatcaaa atactagctg
    16201 tcgccagtaa tttttcttcc ctgtaaacac caaacttctc atgttgccaa ttagacttaa
    16261 cttcctccca agcactactt tgtaatacat tggctaattc atgttctttg acaaactgat
    16321 catattctaa tgtgggaatg ccaatttgat aacggtacat ttcctactct ctttcctcca
    16381 gtattttatt actattctac tactttcgtt tgaaaagtct agtgaaaaca tattttattt
    16441 tagttctact ccccctatgc tgaagttcaa actaagtcag tctctcttct aatttgaaat
    16501 caattggtag agttgttaaa aaaacttcca attgtttttc ccagtagtac cactcgtgag
    16561 ttccagcgct atggctatag gtcacatcaa aacctagttt tttgagattt ttcactgcga
    16621 gattattggc ttcgtacaag aaatcctgtt cgccacacca cgcccaaagt ttggtctttt
    16681 tatccgattt tttagccaga ctttcaagag aatagggact agttgtccag tctctaatct
    16741 ctccaaaaac acctctccag taggctggac ttcccagatt ttgactttca ggagaaaagt
    16801 tttgaaagct gagggcacct gaaaaactag ctgcatgaga aaaacgattt gtcgcaagag
    16861 ccagtttgaa acagccgtag cctcccatag aaagaccagc tataaaggtc ttttcacgct
    16921 tgctagtcat attagggaag aagcgtttca gaacctgtgg caattcctct gctagagccg
    16981 tgtagtagtc aaaaccatac tgggtatcgg tgtaccaacc attgctggta ttgggcataa
    17041 caacgatgag attagttcct cgaagcaagc gttctacatt ggtccgctta agccaactat
    17101 tatgatttcc agacatcccg tgcaaaaggt acaagacggg aatatcttca cactctggtt
    17161 cttccactcg attggcatca gggtagagga cattcatccc ccactccata tccaatactt
    17221 gtgagtaata ctcgattttc attactgcca tgattttctc cttctatttt tctataagaa
    17281 aaagccgaaa cttgactttc tctctgttta tttcaacgat tattttgctt ccatgactac
    17341 tggtaaaata gctggacgac gcttggtttg gtcaaagaga tacttggtca aattatctcg
    17401 aaccttccct ttaagatctg cccagtcaaa gtcatctcct tgaagataat cttctaccgt
    17461 ttggttaatc aattctgaac tttcacggag aatatcgcga ctcttcttga gataaacaaa
    17521 tccacgcgtg tgaacacgag ctctagccac aattttcttc tcacgacggt tgactgtaat
    17581 agccacgatg aaaattccat cctctgacaa gaccttacgg tcacgaagaa caacatttcc
    17641 aacatcacca atggcattcc catcaatcaa gatatctcct gctgaaaccg atccagctgg
    17701 aacgaagtct ccattctcgt aagacatggt cgtccccttt ttaggaatga agatgcgttc
    17761 tggcaacatc ccaactgcca tggcagcctt agcgtgagca tccaactcac gatactcccc
    17821 ttggacaggg aagaggtact taggttgcaa aagattgatc atcagctgca aatcacgcac
    17881 atttccgtgc cctgatacat gtaaactttg ggtaatcaat ttgacaaccc cacctgcctg
    17941 ataaatcata ttttccacac gcgcaacaaa ggcttcttta gcaatagacg gagccgtagc
    18001 aatatagact aggtccccat ccttgatttc tacataacga tggcgaccaa tcgacatctt
    18061 acgaagtcca ttgataggct cacccatacg acctgtctca agaataatca actcatggtc
    18121 ttcaaagcga gacatatctt taggcttaat caaaagaatt tcgttggcta aagacaactt
    18181 cttaagacga atcgctgtgc ggacgatatt ttcaatatca aatcctgtca agacgatacg
    18241 tcgacctgtt ttatccgcag cgtcaaaaat ctgctgaata cgagaaagat tactggaaac
    18301 agctgcaacg atgatacgac cttcccagtc agcaatagtt tgggtaattt catccctaac
    18361 ttcactttca ctagccacct gaatattgct gtctgcattg gccgaatcac tgaggagagc
    18421 caggacgccg tcacgaccaa tctctgccaa acgagcaaaa tcagttgcat aagattcact
    18481 agccgtttgg tcaaatttga agtcacctgt ataaacgatg cttccttccg atgtcttcaa
    18541 gacaattccc agactctctg gaacggagta agtcgtaggg aagaaggaaa ccactgtccc
    18601 accaaaatca atctccgtat tctcatcaat gacatggaaa tcattaaatt tcttaacggc
    18661 atcatttcct ttgacaaaga gctttgccaa ctcaatggtc aactcagacc caaatacagg
    18721 aactttagcc tctgccaaga gatacggtag agcaccaatg gcatccgcat gcccgtgggt
    18781 caagaaaacc ccagcaatac ggtcgctatt ttcaaaaagg taatccatgt ttggaatcac
    18841 cacatcgacc cctaattgtt cattttcagg atattttaac cctacattca aaacaaaaat
    18901 ggactctcca atttcagcaa tgtacatatt ttttccattc tcacgcacac caccaagtgt
    18961 tgttaaactg atattactca tttttcctcc aaaatcttag tttatcttga ttatagggca
    19021 attctaccct cttattctaa aagttctgat actttcagcc gaatcttttc ctaccattat
    19081 accatttttt tgatgatttt caaaatgaag gtctgttttc aaaaaagagt tgatttccca
    19141 actctttcct aacttctact ctttttccat caaaaaatca taaatttctt gcaccgtacc
    19201 cagcgccatc atttcttggt ctttaacggt ttgtttgaga ttttggtaaa tttgactttc
    19261 tcctatctcc cgctttggcg cctctttatt gactgtcatc acaccatctt ttatcgtcac
    19321 aaagcctagt tcttcaaata cttgaatcat cttgaccagc aagatttgtt gaatattaag
    19381 atatgcagcc aaatctttca gcttgtagcg aatatcaaac tctgggaact ggtaaatagt
    19441 cttgtacaat ttggcaaact gatctctagt cccataacct gtcagataat aagccttgtc
    19501 aatatcattt ttgaaataga cagcagagaa atgctgttcc tgaaaaatgg tcttcagctg
    19561 agtaatatcc tctggaatgt tttttacgac aacagcttca ctagccgcaa gatttggcag
    19621 ttctccagga aaatccaaga ctggaacacc ttctggcaag actgcatttt ttccacgaat
    19681 gttaaaaagt tgaacacctt ccactcgcgc atccaccatc atcaactgga gggcagtttg
    19741 gccattccat tggttgacag acaatttaac cgctaactct agattcttgg tttgagaaaa
    19801 ctctgtcgcc catctgcctt gaccaaaggc taccacttca aaactcgcct cacccttgga
    19861 aattttcagc tttagatggg cattacctgc ccccatagta cgagcacttt cgacctgaaa
    19921 attcttgata taaaaaatag gtttctgatt atccattcca aaaggagcta aacgttcaaa
    19981 acttttgacc gtttccaagc taagtgcctc caaatccaac tcttcatcta ggtttaactt
    20041 attcttgcca ccagcatctg cacctttttc acgaacataa tcttccaaaa cctgagataa
    20101 atctgagagt tgctcaactt ccagcgtcat acccgctgca cctgcatgac ctccaaaggc
    20161 gatgaagagg tctcgatagg gatccagagc ttcaaaaata tcgaccgctt ccacactacg
    20221 agcactgccc ttggcacgac cgtcttctat attaagaaca atgactgtct gtcccaattc
    20281 ttccaataaa cgaccagcca cgattcctag aaccccagga ttccagcctt ccttggccaa
    20341 gacctgaacc ttcttctcag gatccaccat ggtcttggct tcttcataga tagactgaac
    20401 gatttccttg cgctcttcgt ttttctggtg aatcataagg gcaatctcat gcgcttcctc
    20461 atcatcaaat ccagtcaaca aatcaatggc aggattggga tcatccaagc ggcccaaggc
    20521 attcaaacga ggagcaatct ggaaaccaac cgtttcttct gttacttcgt tagcagcaat
    20581 cccagccatg tccagcattt cttgcagacc aatgcgctgg gtatgaccca acatttccag
    20641 accatattga actaagatac gattttcatc cgtcagactc accatatctg caatagttcc
    20701 aatagcgacc aaatcaagca attccacttg cacttcttct aacagggcac aagccaactt
    20761 gaaagcaact ccacaaccag ccaaatattt aaaaggataa tccgcatctg gatgttcagg
    20821 atggacaata gcataagcat ctggcagggt ttcaggcatg gaatgatggt ctgtcacaat
    20881 gacatctact cccatagact gagccaatgc aatagcctca tgaccagcaa ccccattgtc
    20941 caccgtcaca atcaaggaaa ttccttcttg ctcgataaag tatttataaa cactagcatt
    21001 agggccatag ccatcggtaa aacgatttgg caggtaaact cggcactccg caccaagttg
    21061 ttccaaactt tccttcacaa tagaagccga agtcatgcca tccgcatcat agtctccata
    21121 aacgagaata ttttcccctt cttcaatagc ctgacgaatc cgctccactg ccttgtccat
    21181 atcatggagc agataagcat catgtaagtc ctccaaggaa ggttctaaaa acttcttcag
    21241 actttcttgg tcctgaatcc ctctctcaaa caataaccga gccacctcag gacccagtcc
    21301 agccttcttg gctatctttg taaaatccgc atcttctacc tgcagggcaa actgccattc
    21361 ataagtaggt gttatcaaaa agacatccac tctttctaag aattctattg cttcattata
    21421 acatgattta gacgttttct ctatccagat tgctttttta taacatttta tagtatattg
    21481 aaactagaat agtacacctc tacttctaaa atattgttag aaatcgattt gactgtcccg
    21541 atcgattttt cctgttctta tttcactata gtgaattttt cagatatggt ttgacaagaa
    21601 cattctttta ttgtagaatc gtgttataaa acctaacata aaggagatct ctcatggctt
    21661 tagtagaatt taaaaacgtc gaaaaatatt acggagacta ccacgcactc cgcaacatca
    21721 atctccgttt tgaaaaagga caagttgttg tcctgcttgg accttctggc tctgggaagt
    21781 ccactcttat ccgtacgatc aatggtttag aggctgttga caaaggaagt ctcctagtca
    21841 atgggcacca agttgctggt gccagccaga aagatttggt acctcttcgc aaggaagtcg
    21901 gcatggtttt tcaacatttt aacctttatc cacacaaaac ggtgttagaa aatgtgacac
    21961 tttcgcccat taaagttcta ggaattgata aaaaagaagc tgaaaaaacc gcccaaaaat
    22021 atctggaatt tgtaaatatg tgggacaaga aagattccta tcccgccatg ctatctggtg
    22081 gacaaaaaca gcggatcgcc atcgctcgtg gtcttgctat gcatccggaa ctcctcctct
    22141 ttgatgaacc aacatctgct cttgatcctg agactatcgg agatgttcta gcagttatgc
    22201 agaaactggc gcatgatggg atgaacatga tcatcgttac ccacgaaatg ggctttgctc
    22261 gagaggttgc ggaccgcatt atctttatgg ccgacggaga agttttagta gatacgacag
    22321 atgtcgataa cttttttgac aatccaagcg aagctcgtgc ccaacaattc ctcagcaaaa
    22381 ttatcaacca cgaaagtgac aaagtcaaat aaggaggcgc ctatgaaaaa aaattctttt
    22441 tatcagcatt attgattagc cttttcggcc ttgctgctgc caaaccagtc caggctgata
    22501 ctagtatcgc agacattcaa aaaagaggcg aactggttgt cggtgtcaaa caagacgttc
    22561 ccaattttgg ttacaaagat cccaagaccg gtacttattc tggtatcgaa accgacttgg
    22621 ccaagatggt agctgatgaa ctcaaggtca agattcgcta tgtgccggtt acagcacaaa
    22681 cccgcggccc ccttctagac aatgaacagg tcgatatgga tatcgcgacc tttaccatca
    22741 cggacgaacg caaaaaactc tacaacttta ccagtcccta ctacacagac gcttctggat
    22801 ttttggtcaa taaatctgcc aaaatcaaaa agattgagga cctaaacggc aaaaccatcg
    22861 gagtcgccca aggttctatc acccaacgcc tgattactga actgggtaaa aagaaaggtc
    22921 tgaagtttaa attcgtcgaa cttggttcct acccagaatt gattacttcc ctgcacgctc
    22981 atcgtatcga taccttttcc gttgaccgct ctattctatc tggctacact agtaaacgga
    23041 cagcactact agatgatagt ttcaagccat ctgactacgg tattgttacc aagaaatcaa
    23101 atacagagct caacgactat cttgatacct tggtcactaa atggagcaag gatggtagtt
    23161 tgcagaaact ttatgaccgt tacaagctca aaccatctag tcataccgca gattaaggag
    23221 gataccctat gacagattta tcatcttgga cagcctattt tcaggatttt ggacaatttt
    23281 tcaatggttt cctctttact cttgccctag cgattggatc ctttatcctc gccatggtct
    23341 taggagtctt ctttggtgcc atgtcaacca gtaaacgtcc aattttacgc attttagctc
    23401 gcatctttgt ggaattttac caaaacactc ccctcttggt gcagtttgtt attgtttttt
    23461 atggcctacc tcttatcagt gaccatacta tcatgattcc gatttattgg acagctgttc
    23521 tctgcgtggg actctatcac ggcgcttata tcgctgaggt tattcgttca gggattcagt
    23581 ctttacatcc tgattgccct catctacttc agctttaacc tagcaatttc taacctgtct
    23641 catatgctag caaaacgttg gcaacaagct gcagaataac aaattagctc tctagttcat
    23701 tggagagttt tttgatgata gtagatattt atagacctca atttgacacc atacattttc
    23761 tatgataaaa tgtaacaaat tcatttacaa caaatcgaaa tctaaatgaa atggaatttt
    23821 ctatgaaatc agtcaaagga ctactcttta tcatagctag ttttatcttg actcttttga
    23881 cttggatgaa cacttctccc caattcatga ttccaggact agctttaaca agcctatctc
    23941 tgacttttat cctagccact cgtctcccac tactagaaag ctggtttcac ggtttggaga
    24001 aggtctacac cgtccacaaa ttcacagcct ttctctccat catcctacta atctttcata
    24061 actttagtat gggcggtttg tggggctctc gcttagctgc tcagtttggt aatcttgcca
    24121 tctatatctt tgccagcatc atccttgtcg cctatttagg caaatacatc caatacgaag
    24181 cttggcgatg gattcaccgc ctggtttacc tagcctatat tttaggactc tttcacatct
    24241 acatgataat gggcaatcgt ctccttacat ttaatcttct aagttttctt gttggtagct
    24301 atgccctttt aggcttacta gctggttttt atatcatttt tctatatcaa aagatttcct
    24361 tcccctatct agggaaaatt acccatctca aacgcttaaa tcacgatact agagaaattc
    24421 aaatccatct tagcagacct ttcaactatc aatcaggaca atttgccttt ctaaagattt
    24481 tccaagaagg ctttgaaagt gctccacatc ccttttctat ctcaggaggt catggtcaaa
    24541 ctctttactt tactgttaaa aattcaggcg accataccaa gaatatctat gataatcttc
    24601 aagccggcag caaagtaacc ctagacagag cttacggaca catgatcata gaagaaggac
    24661 gagaaaatca ggtttggatt gctggaggta ttgggatcac ccccttcatc tcttacatcc
    24721 gtgaacatcc tattttagat aaacgggttc acttctacta tagcttccgt ggagaagaaa
    24781 atgcagtcta ccttgattta ctccgtgact atgctcagaa aaatcctaat tttgaactcc
    24841 atctaatcga cagtacaaaa gacggctatc ttaattttga acaaaaagaa gtgcccgaac
    24901 atgcaaccgt ctatatgtgt ggtcctattt ctatgatgaa ggcacttgcc aaacagatta
    24961 agaaacaaaa tccaaaaacg gagcttattt acgaaggatt caagttcaaa taagtaatca
    25021 tactctcctg gaatttccca ggagagtttt ttatagccaa atgaaataag accggaacga
    25081 tggaattttg gcattcaaat caattgataa gaatatttta tagtggagtg aaataggata
    25141 cgaacaaatt gattaggaaa atcaaatgaa tttatagaaa tcttttagca gttatgttat
    25201 cctattctag tttcaaaacg ctatagaagc agcattgtgc tagtctagat tcagtttact
    25261 atactaaaac gagtagcttg aaatcaaaaa acccaccctc acaggcaggt tttatctgta
    25321 ttattcagct agattatgct ttaccttctg aaccgaatac gtcgatacgt tcttcaaccg
    25381 atgcttggat agcttttaca ccgtcagcca agaatttacg tgggtcgaag agttttttct
    25441 tgtcgtattc tgcttcgttt gcttcgtagt cacgagcaaa tttacgagtt gcgttagcga
    25501 atgcgatttg gcattctgtg ttaacgttaa ctttggcaac accaagtttg atagctgctt
    25561 ggatttgctc atcaggaata cctgatccac cgtgcaatac gattgggaat cctggaagag
    25621 cttctgtcaa tttttgcaag tggtcaaggt caagaccttc ccagtttact gggtaaggac
    25681 cgtggatgtt accgatacca gctgccaaga agtcgatacc agtttcaacc attgctttag
    25741 cgtcttcgat tggagccaat tcacctttac cgatgattcc gtcttcttca ccaccgatag
    25801 taccaacttc agcttctact gagatacctt tagcgtgtgc tttttcaaca acttctttag
    25861 ccaatttaag attttcttca actggaaggt gtgaaccgtc aaacatgatt gaagtataac
    25921 caacttcgat acactcaagt gcatcttcgt agtgaccgtg gtcaaggtgg atagctactg
    25981 gtacagtgat acccattgat tcaacaaggt tagcgatcaa gttgcgagca actttgtaac
    26041 cacccatgta tttagcagca cccattgaag tttggatcaa aactggagct tttttagctt
    26101 ctgctgcgcg caagatagct tgagtccact caaggttgtt tgtgttaaat ccaccaactg
    26161 cataaccgtt gtcacgggct gcttggacaa atttttctgc tgaaacgatt gccattttat
    26221 caggcctcct gtatattttt atgggtcatc ccatttacat tgttcatttt atcacttttt
    26281 gccaaaaaaa tctagttttt cccgcagttt cgattgattt tcttctaact ccatctatgt
    26341 aaaccctttc tctccctagt cttgggcgac ttttggaaaa tctataaaga aggttaaacg
    26401 attctcctcc atctcgaaac gataagctaa tttttcatgt tctaatagac tcttaaccac
    26461 aaagagcccc ataccagacc ccttgacctt gcgactagca ttgtcagaaa aagactgggc
    26521 tagtttttct tgttcctctg agctacagct attttcgata aaaagttctc cttctctttc
    26581 tccaattcga actaagccac ctggaacaga gtgcttaatg gcattgctga tgagattaga
    26641 aagaatcaac ttcataactg atgggtttag ataagcctgc tgatgggtca aactattgtc
    26701 tatctggagc tctctttcct tggctagcaa ggcataatct ttgaccagat tttgcgtcat
    26761 ctggaggagg tcaattgttt ccctatcatc tcgcaattcc tgcacagaag agagggaaag
    26821 tatctgcaga acatggtgat tgagttcatc cacaatcccc aaggcaactc ccagatactg
    26881 gtctctatcc ttataacgac cgatattctc tctcatattt tcgattagga ttttcaaact
    26941 agccagcggt gttttcaatt catgagaagc tcctcgtagg aattcgacct tcatcttctc
    27001 cagctggaga atggcttcat tcttttcatg caagtccgca ataacagtca agagatgctg
    27061 gtagaggcta ttgatttgtt ccttgagatt acctatctca tccttagaat ccacgcgcaa
    27121 tcgcacttgg gaatccaggt ccatcatccg acgggtcacc cgcttgattt ccaaaatcgg
    27181 tgcaacaata gtccgagcgt agatgtaggc caccaagagg gaaatcagaa aggaggccag
    27241 caaggtatag ggaagaaact ggagactgat ttgctccgct tccttttgta aatccatgga
    27301 agctagaaac tggagaatca tagtaccacc gtcttgcgtt ttcacctcgc gctcctcaat
    27361 aaagagagag gttgtctggc ggtctgtgtc cagaggaaga ctgtccttga cttctaactt
    27421 gtcctcggtc atctcacctt tgacggtccc cttgatatca ctagtctggg aatacaaatc
    27481 taacacttgc tcgatactct gcctatcttt cccttctagg gactgggcaa tggctgttgc
    27541 cttttgacca atggtttcct gacgatgact cagataagtc gaaggaaaaa gaaaataaat
    27601 agctaaatga aggcagataa ccagaacact aaatatcgag aaggtataga taaatatctt
    27661 tgcaaataaa cctgttcgtt tcattttcgc tccaatttat aaccaacatt gcgcacagtg
    27721 aggatacaat ccaagtctag ctttttccgc aattccttga tataaacatc aataacacgg
    27781 tcaaagggaa cctcatctgt cgctttccag acggcatcga taatctgaga ccgagtcaag
    27841 gcccggcctt catttttcac tagatagtcc agaatttcca actctttggc attgataggc
    27901 acttcttgac ctgcgaggct tgcactgtag ctttcaaagt ccaccttggt atccttgtaa
    27961 gaaaagattc gtcctgtatc gtagtagcgc ttgaaaatcg cgtccaccct cacttttaag
    28021 agggagaggg agaaaggttt ttccagatag ccatctgcca aagaggcaaa ggcactcatc
    28081 ttgtattcct catcctgaaa agctgtcaac atcaagacag gaacctgact ggttttacga
    28141 atctcagcta gaacttctaa gccgttaagc ttgggcatct ggatatccag taaaaccagg
    28201 gccacctcat agctagaaaa ttgctccaga gcttcctgac cgtccgctgc ctcaatagtt
    28261 tcatagccac aatccgtcaa ataatcactg accccctcac ggatcatctc ttcatcttct
    28321 acaattaaaa ttttcatact ttaactgctc tctatttttt atttttctta gaataaatac
    28381 ctactctatt ttctattata gtctcttgct ggccttttgt ctgtaagtaa ctgatcacta
    28441 gataaaacgt tgtgaaattc ctttctcata aattccatag ctttagtata ttatatttaa
    28501 gcaataaagt acaaagaaaa caactgaaag caatgatttt caccactgct ttcggattta
    28561 ttttgaattg ttaaatagcc attcctatcc actattcttg aatagaaaca caagatgcaa
    28621 tctttattcc agactcattt tttcaaattt attcaccatc cagcaagagc tcttttggtt
    28681 gttttctaag gagattgctt gaagcaagcg ccataacgag aaccactaga accaaggcaa
    28741 ggacaaaaat gatgataaag tctgatgtct gaatagaaat gtctaggctc gacaaggtct
    28801 tgctaaagcc atctacttct gcaccaccac caaggttaga ggcttgagcc gccttactag
    28861 cctgtttggc aacacctgaa gtcacattgg caaggacagt gtttccaatt gcacgggcag
    28921 tgtaattagc taggaagtaa gcagaaacta gagcagggat agcaatcaag atagattcgg
    28981 tgatgaattg acccaagata cttgcctgct tgaggccgat agagaggaga attcccactt
    29041 ccttgcgacg ggcgttgatc caaaggctga gcaagagggc aaggaggaga actgagaagc
    29101 tcaagctacc ccagaagagg aggttggcca tcttgtacat accagatata gattgctcaa
    29161 gagctgggta gttagaggag ctcttgacga gtgtgtagct cttccagttg ataccactga
    29221 tgccattcaa ctctttcata acatcatcca agttcttgtc tgctgttaca aagaaggttg
    29281 cgtccccata aatggctgtg tcttctgtgt atccataaag ttttgcagca gtgtgaatgt
    29341 ctgtaatagc tgtgttttcg taaagttctt gtgagtaggt tactgctgac ttattatgac
    29401 catcaaagag tcccttgatt gtcacttcaa ctgtttcctt ggctcctttt tcattatctg
    29461 catcgtagat attagagtcc agtttaacct tgtcccctac tttccagccg tgtttggctg
    29521 ccaagtcctt gtgcaagagg attttatcct tgtcgtcgtt ggttaagtgc tctccttcga
    29581 ctagtttata agaaccagag acaaacttgt cttctttaga ggagtcattg acacctgtaa
    29641 tcatcaagct acttccaaaa cgtttggcac gatcagcagt gagattcttc ttgatttctg
    29701 gcgtttcaat caggtcatat ccagtcaaat ctccgatagc gttgatacgt ttgacataag
    29761 actcaatggc cttgttttcg gtgatttttt tgatgtcttc acccttgata ttcccagcac
    29821 cgcgaggcgt tccttggttg acgcgacgat tgatttgcat ggagaagcta ttggtgatat
    29881 ttttaaaggt ctcctgagaa gccttggcag tagctccctt gattgacaag ccgaccaaac
    29941 tcaagctcgc catgaggaga ataatcagga agatgacaat cgatttgaaa aacttccttg
    30001 taacataggc aaatgcgttg tgtaacatag attccctttc tagattttgt tttaatcatt
    30061 ctatcaaaat aagctcaaat tatttactag tattgcgcgt ttcagtcagt ttcttatcct
    30121 ttaattcaag tgtaatatct gacgcttgtg ccacttcttt actgtgagtt acgacaatca
    30181 cacatttacc tgttttctgg gcaagtgatt tgagtagttc gacaatatct ccagcagttt
    30241 taggatccag atttcctgtt ggctcatcag ctagaataac tggagcttct gagaccaaac
    30301 tgcgagcaat ggcaacacgt tgctgttgac cacctgataa ctggagaaca ttccgcttga
    30361 tctggctttc atccaaacca agctcaagaa gtgtattctt gcttgccttt ttgttgacca
    30421 atcggatatt ttccagcgga gaaagataat ctatcaagtt ataattttga aagaccaggg
    30481 aaatatggtg catgcgatgg taagaatagc ccttcttacg aatatcctct ccttgaaaaa
    30541 ggatagaacc ttcaacagga ctatctagac cagcaagtag ggacaagagt gtggattttc
    30601 ctgctcctga ctccccaata atactgtaaa attttccggg ttcaaaatta taattgatct
    30661 gatataggac tgcttcagca gtattcttat aacggtaggt aacatcttgt aattgtaata
    30721 aagtcatgat ttctccttct taactaatag atgataaaat ttctttcggt gattttctaa
    30781 ataagaatag gaaacaaagg gctacagata agcaactaag cagaactaga aaaacatagg
    30841 attctgcaaa agataagata ctagttgata aactgcttgc tttggctagt gtatcttgta
    30901 agcttgcctg atctccactt gctagtagag tttggagtag gtaagttgtg attccgtttc
    30961 ctgcaacaaa tgctggaagc aaagctccaa gagataccaa aactacctct aaacagaatt
    31021 gtaggaagat cgagctcttg ccttttccaa gtgcaagtaa aatccccact tcatagaccc
    31081 gttctctcaa ccagagagac aaaaccagaa ttaaggctcc agctcctgct atcaacatcc
    31141 cataaaggaa gatggtcagg aaggtttgga aagttgcaac tgagtctttg atttgttcaa
    31201 aagccttgtt ttccttttcg acttggtagc cttgattttc caaggccaag ttttctacct
    31261 gcttcatgag tccgtccatt tccttaggat tttctacata gaagcgtgct gcactgactt
    31321 gagcttcact attgcccaaa agggtttggc tactttcata gtctgtaaag acttgatttt
    31381 cactgaagtc agaagacaag cctgtgaatt tctcttgttt tttaccagaa aagatgccaa
    31441 taatctcaaa ctctactgtt tgtccttttc cagattcaga ctgaccagca tccaagccaa
    31501 tcttgtcatg aagtgaaaga ccgttcttct tagccaattc ttcgtggata aggattttct
    31561 tggaatcccc tttttgaagg tgtcgccctt cttttagatt gaaagccgaa ctggtaaagg
    31621 ttacatcctt ggatgaatcc tcaagagccg ttaagctaac caagttattg tctgcagctg
    31681 ataaatcatc acgctccacg ctctgctcgc cagtcactgc ttccttgtct tttagttttg
    31741 cgaccgtctc aagttcagga gagacatttt ccagcccctt aatcttgctt acagatgcta
    31801 ggtctgacaa cttgaatgtc tgaccattct ctatcttctt aatagaaaaa gatgtattga
    31861 gtgatttata aagattgctt tctactgttt tgttggactt catcagagtc aaacaggctg
    31921 aaattccggc caataagacc aataaaatca gaaataaaat aaaacttctc agtcgctttc
    31981 tgctgacata agcccaagat ctttggattg gattcatttg tcacctccat atttgtaaga
    32041 ctattataaa acccaaatat gaaatgttta tgaaatacga aaaaaaatat cgagtagggg
    32101 ataatctctc gcccctctca caccaccgta cgtgccgttc ggcatacggc ggttcaacta
    32161 acttttaacg catgtcgttc aaggtaataa tccaaacacg aaacctgttc acgtttttca
    32221 aggactggtt ttgatatagc acgtttaagt accgacttct gagctactat agtagattga
    32281 aactagaata gtacatctct tcttctaaaa cattgttaga aatcgatttg acttccctga
    32341 tcgattcgtc ctattcttat ttcattttaa tataattgat a
//
LOCUS       DAVKGU010000017        31014 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKGU010000017 DAVKGU010000000
VERSION     DAVKGU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.017"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..401
                     /locus_tag="VW077_000719"
     CDS             <1..401
                     /locus_tag="VW077_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9454782.1"
                     /translation="KNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSGFTDADYQKQ
                     GAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPELADAMLAAKT
                     TGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            402..815
                     /locus_tag="VW077_000720"
     CDS             402..815
                     /locus_tag="VW077_000720"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9454783.1"
                     /translation="MGAHFLTKQAGGSGVLLGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLMSNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            761..1087
                     /locus_tag="VW077_000721"
     CDS             761..1087
                     /locus_tag="VW077_000721"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454784.1"
                     /translation="MVDIAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(1454..1714)
                     /locus_tag="VW077_000722"
                     /pseudo
     CDS             complement(1454..1714)
                     /locus_tag="VW077_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(1717..2217)
                     /locus_tag="VW077_000723"
     CDS             complement(1717..2217)
                     /locus_tag="VW077_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9454785.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            2253..2432
                     /locus_tag="VW077_000724"
     CDS             2253..2432
                     /locus_tag="VW077_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454786.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            complement(2410..3378)
                     /locus_tag="VW077_000725"
     CDS             complement(2410..3378)
                     /locus_tag="VW077_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9454787.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(3464..3679)
                     /locus_tag="VW077_000726"
     CDS             complement(3464..3679)
                     /locus_tag="VW077_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9454788.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDAIWQEYLEH"
     gene            complement(3688..4542)
                     /gene="cvfB"
                     /locus_tag="VW077_000727"
     CDS             complement(3688..4542)
                     /gene="cvfB"
                     /locus_tag="VW077_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9454789.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(4602..5159)
                     /gene="frr"
                     /locus_tag="VW077_000728"
     CDS             complement(4602..5159)
                     /gene="frr"
                     /locus_tag="VW077_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9454790.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKREKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(5168..5905)
                     /locus_tag="VW077_000729"
     CDS             complement(5168..5905)
                     /locus_tag="VW077_000729"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9454791.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQPGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(5989..7323)
                     /gene="trmFO"
                     /locus_tag="VW077_000730"
     CDS             complement(5989..7323)
                     /gene="trmFO"
                     /locus_tag="VW077_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9454792.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGAGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(7568..7808)
                     /locus_tag="VW077_000731"
                     /pseudo
     CDS             complement(7568..7808)
                     /locus_tag="VW077_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
     gene            7818..8622
                     /locus_tag="VW077_000732"
     CDS             join(7818..8186,8188..8622)
                     /locus_tag="VW077_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9454793.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLHYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGFTISR
                     TPLSSEDTVMIDATKVKINRPKKELANDSGKKKCHAMKSQAIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLIAEDKAYNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(8611..9258)
                     /locus_tag="VW077_000733"
     CDS             complement(8611..9258)
                     /locus_tag="VW077_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9454794.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(9367..9885)
                     /locus_tag="VW077_000734"
     CDS             complement(9367..9885)
                     /locus_tag="VW077_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9454795.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(10112..10981)
                     /gene="rsmI"
                     /locus_tag="VW077_000735"
     CDS             complement(10112..10981)
                     /gene="rsmI"
                     /locus_tag="VW077_000735"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9454796.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASKGVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(10984..11301)
                     /gene="yabA"
                     /locus_tag="VW077_000736"
     CDS             complement(10984..11301)
                     /gene="yabA"
                     /locus_tag="VW077_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9454797.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(11341..12231)
                     /locus_tag="VW077_000737"
     CDS             complement(11341..12231)
                     /locus_tag="VW077_000737"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9454798.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLTKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(12228..12866)
                     /locus_tag="VW077_000738"
     CDS             complement(12228..12866)
                     /locus_tag="VW077_000738"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9454799.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(13088..13885)
                     /gene="proC"
                     /locus_tag="VW077_000739"
     CDS             complement(13088..13885)
                     /gene="proC"
                     /locus_tag="VW077_000739"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9454800.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            complement(13889..15151)
                     /locus_tag="VW077_000740"
     CDS             complement(13889..15151)
                     /locus_tag="VW077_000740"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9454801.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(15161..16291)
                     /locus_tag="VW077_000741"
     CDS             complement(15161..16291)
                     /locus_tag="VW077_000741"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9454802.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(16371..18254)
                     /gene="cbpE"
                     /locus_tag="VW077_000742"
                     /gene_synonym="pce"
     CDS             complement(16371..18254)
                     /gene="cbpE"
                     /locus_tag="VW077_000742"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9454803.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWRQGINTTYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(18257..19144)
                     /locus_tag="VW077_000743"
     CDS             complement(18257..19144)
                     /locus_tag="VW077_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9454804.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(19134..19595)
                     /locus_tag="VW077_000744"
     CDS             complement(19134..19595)
                     /locus_tag="VW077_000744"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9454805.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(19592..20500)
                     /locus_tag="VW077_000745"
     CDS             complement(19592..20500)
                     /locus_tag="VW077_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9454806.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKDELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            20493..20690
                     /locus_tag="VW077_000746"
                     /pseudo
     CDS             20493..20690
                     /locus_tag="VW077_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(20742..21413)
                     /locus_tag="VW077_000747"
     CDS             complement(20742..21413)
                     /locus_tag="VW077_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9454807.1"
                     /translation="MKKRAIQILLVLSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISPPS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVIMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(21467..21688)
                     /locus_tag="VW077_000748"
     CDS             complement(21467..21688)
                     /locus_tag="VW077_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9454808.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLL
                     VHGFCPRGTDDLGYIGGGNHLCKGSAARF"
     gene            21960..22769
                     /locus_tag="VW077_000749"
     CDS             21960..22769
                     /locus_tag="VW077_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9454809.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(22950..23825)
                     /gene="aguB"
                     /locus_tag="VW077_000750"
     CDS             complement(22950..23825)
                     /gene="aguB"
                     /locus_tag="VW077_000750"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9454810.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELFFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(23835..24920)
                     /gene="aguA"
                     /locus_tag="VW077_000751"
     CDS             complement(23835..24920)
                     /gene="aguA"
                     /locus_tag="VW077_000751"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9454811.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(24917..26044)
                     /gene="nspC"
                     /locus_tag="VW077_000752"
     CDS             complement(24917..26044)
                     /gene="nspC"
                     /locus_tag="VW077_000752"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9454812.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKEVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(26044..27303)
                     /locus_tag="VW077_000753"
     CDS             complement(26044..27303)
                     /locus_tag="VW077_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9454813.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(27300..28160)
                     /gene="speE"
                     /locus_tag="VW077_000754"
     CDS             complement(27300..28160)
                     /gene="speE"
                     /locus_tag="VW077_000754"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9454814.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            28541..29130
                     /locus_tag="VW077_000755"
                     /pseudo
     CDS             28541..29130
                     /locus_tag="VW077_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(29096..30571)
                     /locus_tag="VW077_000756"
     CDS             complement(29096..30571)
                     /locus_tag="VW077_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9454815.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         8970 a         7082 c         5687 g         9275 t
ORIGIN      
        1 ttaaaaataa cgaaaaccgt gtcgccctca cacctgcagg tgttcatagc ttagttagtc
       61 gtggtcatcg tgtccttatc gaaacaaatg ctggtctcgg ttctggcttt actgatgctg
      121 actatcaaaa gcaaggagct gagattgtcg ctactgctgg tgaagcttgg gcagcagagt
      181 tggttgtgaa agtaaaagaa cctttaagtt ctgaatacgg ttacttgcgc gacgatcttc
      241 ttctcttcac ctacttgcac atggccgctg ctccagaatt agcagatgct atgttagcag
      301 caaaaacaac aggaattgcc tatgaaactg ttcgtgacaa tcaaggacaa ctaccgctcc
      361 tcgttcctat gagtgaggtt gcaggtcgta tggctgttta aatcggagct cacttcctta
      421 ctaagcaagc tggtggctct ggtgttctac ttggtggtgt accaggtgtt ccaaaaggaa
      481 aagtaactat catcggtgat ggtgtcgtcg gtacacatgc tgcccgcatc gcccttggtc
      541 ttggtgctca agtgactatt ttagatatta gtgccaagcg tctctcagtt ctagaagaag
      601 tctttggaag tcaaattcaa actcttatgt ctaattcatt caacattgaa gcaagtgtga
      661 gagatgctga tgtggtgatt ggagccattc tcatccctgg tgcaaaagca ccggaattgg
      721 tgacagatga gatggtcaaa caaatgcgtc caggctctgt atcgttgaca ttgctgttga
      781 ccaaggtggc cttatcgaaa cagctgaccg tgtgacaacg cacgatgaac ccgtctatga
      841 aaaacacggt gttctccact atgccgttgc caatatccct ggtgcggttg cccgcacttc
      901 aaccatcgcc ctaaccaatg tcactcttcc ttatatcgaa gctttggctg gcaaaggatt
      961 cgcacaagca atctctgaag atgaaggctt gcgtcaaggt gtgaccactt atcaaggtta
     1021 cttgactagc ctaccagttg ctcaaggact taatcgtgac tacactgata tcaatgattt
     1081 agtataaatt ttgaaaagaa aagggtgact tcgcaagtcc tacccactgt gctttgtgtc
     1141 atcagctatt acatcgttgg tttattgtac tgtatacttt gcaactggag actttcgttc
     1201 cagactcaat tgtgcataag aaactctaaa ttgaataaag tcaacagagt ttcgcccaac
     1261 cacttcgtct tgtaatgaca attatataaa ccaaaggaag ctgagctctt tagtcccagc
     1321 ttctttttat atataaaatt ttacccgtgc aaagacaggg ccttagcaga cttctttttt
     1381 acttcgttca cccttgcttt ttctttttag gtttgggcgt tggcagttgg ttatacatag
     1441 ctaaaatcag gtcttataga aacatcttat tatcaagttc ttccactcaa atcatttctt
     1501 tggcaccttt gtatggaaac tcaaaagaag attggtcaat cttatctaag actgcttgca
     1561 cgggtttaac taaaaagcga tcgtcataaa tgccgccaat aatcttgccg cggaagtaaa
     1621 gaatatactc ccccatcatg gaacggtaag tcacatcatc taatcctgat aattgttcca
     1681 aaacaaattc caaatagttc ttacttgatg ccatttctaa tcttctaggc tctgttcagc
     1741 gataacaacc gtatagagtt cttgcttaac ctcgcatcca attgatttaa agccctgctt
     1801 ttcccaaaaa tgctgagatt gcggatttcc cttaacataa gccaaacgtg cctttcgaaa
     1861 gttcttagca aaataagcta gtgcttctgt cacaatatga ctaccaatcc cttttctctg
     1921 ataggcttga tcaaccataa acaaaccaat aaaaacagtc tcctcatcag gatatgcata
     1981 gacaaaatcc ataacagcca caaggtcaga tccattccaa aatccaacaa aaaacttatc
     2041 agccttagct ttaccttcag gtagacaaag catgtcctct tttacagttg caaaatttgg
     2101 ctctggtgga caatgctgaa aatacagagg attactttca tataaagata aaatacttgg
     2161 aatatccttt tcagttagta tcctacaact gtaatactta gatagttggt caatcatctt
     2221 ttcaaattcc tactttcttg tgccctgtga ttatgacaca ggaagatgca ctgatcgtca
     2281 tcagccacat aaaagttctt tccatcgtgc ctaatcgttg tctcagacct ttggataaaa
     2341 cctttagcct atacaactgg attttcctct ctcaaaagta tattcttttg caggcgaact
     2401 tcctcaaaat cagtcgtgtg caacttcagt agaatattca taggctcgga taatctgagc
     2461 gacaactgga tggcgaacca catccttggc tgaaaaatga acaaagtcaa tctgatggat
     2521 gttcttgagt ttctcttgag catcaatcaa accggacttg acattacgtg gcaggtcaat
     2581 ctgactgata tctccattga caatcatctt agaatgaaag cctaaacgcg tcaagaacat
     2641 cttcatctgc atgatggtcg tgttttgcgc ctcatcgaga atgacaaagg catcatccaa
     2701 ggtccggcca cgcatatagg caaggggcgc aatttcgata atttcacgct ccatgagacg
     2761 agtcgtttgg tctttcccca gaatctgata caaggcatca taaacaggtc taaggtaagg
     2821 atctaccttc tccttaagat cacccggaag aaaacctaga ctctctcctg cttccaccgc
     2881 tggacgtgtc aagataatcc gcttgacttg cccacgttta agggcagtca ctgccaaagt
     2941 aactgcaaga aaggtcttcc ccgtccctgc aggcccaatt ccaaaggtca catcatgctg
     3001 tttgacactg tccacataaa gtttttgacc taacgtttta acgcggatcg gtttcccagt
     3061 attatccttg ataatttctt cttcgtaaag ggcgacaaac ttatcaattt catcgttttt
     3121 gaccatgcta attgcagtca ccacatctgg cgtaccaacg gtcatcccac gatttaccaa
     3181 gaccatcaaa gcctgaataa cttgacgggc ttcctcacag gcagactctt ctcccaaaac
     3241 ctggacaatc tccgtacgag catgaatcac aacatcaagc tcttcttcca tcaaacgaag
     3301 atggcgttca ttggaaccaa aaagatgaaa caggtcatct ggatgactca gttgaatgtc
     3361 tattgaatgt tccttcaaat aaagaacctc tctaatcctt ttatttcttt ttattatagc
     3421 agagggatgg agaaattact agcccaaacc tagtgaataa atgctagtgt tctaagtatt
     3481 cttgccagat agcgtcaaag tctcctaggt taaaagagaa actggcatgc tcctccaaaa
     3541 agcgactgac ctcatcaaaa tcatctgtgt gttttggaaa agctgcctct tcaaaagcga
     3601 ggtctgccaa aattgctttg ggactgttac ttttaggatt gcgctcggtc atgagccaag
     3661 tgtaaaatga ttttctcata agcctcccta aatcaactct gtcccaaact ggtcctgctt
     3721 gattttacca gccttcataa gaccacctaa agctttcttg aactgacctt tagaaatgcc
     3781 aaaggttgcc ttgatgtcgt ctggagatga cttatcattt aaggtcatga aaccgccatt
     3841 actttccaaa taagtcaaaa tcatctgagc atcgttttcc aacatttcaa aggagcgtgg
     3901 tttgagggag aggttcagag tgcggtccac ttcacggaaa ccaataacgc gcgcatctaa
     3961 tacttgcccc aaacgtggct ctgcgtaacg ctcgctagga tgaataaaac caagcatatt
     4021 attttctggt aggtaaacaa aagttcctga cagcttgaga cggtaaacaa tggctggcca
     4081 gttttggttc tgcatgttgt tgtaggcagg acgagcaaga cgttggaagt cttcttgata
     4141 agccaagagg ccccagatac ggtctttctt atccacttca agacggatgt agagttggtc
     4201 gcccttctta ggccagagtt ccttgagctc agggagaata tcgagtgaca caacgatttc
     4261 cttgtcagga aggcctgtat ccacaaagac acccaagtcc ttacgaacct ctgtgacacg
     4321 tccccaacca aattggtcct gagtggcagt cacttctaag gttgtcaggc ggagtttttg
     4381 cttcatatcc gtgtatgcaa aacctttgac cgtatcccct actgtatgtt ggccttcttc
     4441 cttagcaaga gcataggttt gaccatcctt ttgcacaaag taaaaacggt cgttttcatc
     4501 gatgatcagt ccaacgataa aacttgcaag atttgtattc atatttcctt ctttcgaata
     4561 aaactcagcc agcaatgcca actgagtttt tctgtttatt tttagacttc caaaagttct
     4621 ttctctttgt tagcagtcat gtcgtcgatg tgtttaacag catcgtctgt tactttttga
     4681 atgtcttttt caagagtctt caattcgtct tcagtgattt cttttgcttt ttctcgtttc
     4741 ttagcttcgt ccatagcatc gcgacggata ttgcggacag ccactttagc attttcgccg
     4801 accttcttca cttctttagc aaggtcacga cgagtttctt ctgtaagagc tgggataacc
     4861 aagcgaatca cagaaccgtc attagccggt gtgataccaa gatcagaagc gttcaaggca
     4921 cgttcgatgt ctttcaatga agacttgtca aatggtgtta ccaacaaaac acgcgcttct
     4981 ggaatcgtaa ttgaagcgat ttggttaaga ggagtttcga ctccatagta ttctacatgt
     5041 acacggtcaa gcaagcttgc attggcacga ccagcacgga taccaccaaa ttcacgagca
     5101 agtgattggt gagactgggt cattctctct ttagcttttt caataattgc gttagccata
     5161 ttctttctta ttccttttct tcgatattat ttgaaactgt tgttccgata ttttcaccaa
     5221 atacgacacg tttgatgttt cctggttggt tcatgttgaa gacaaccaag tcaatgtcgt
     5281 tgtccattga gagggttgag gctgttgagt ccatgatacg aagacctttg ttgataacat
     5341 cacggtgggt caattcttca aacttaacgg ctgtcttgtc cttcttagga tcggcattgt
     5401 acacaccatc gacgccattt ttagccatga ggatggcatc tgcttcgatt tcagctgcgc
     5461 gaagagccgc tgttgtatct gtcgagaagt atggtgaacc aattccagca ccaaagataa
     5521 cgatacggcc tttttcaagg tgacgaaggg cacgtccacg gacataaggc tctgccactt
     5581 gttgcatggc aatagctgtt tgtacacgcg tatcaacccc aacttgttgc aatgaatctg
     5641 ccatcacaag agcattcata acagtcccaa gcattccagt gtaatctgcc tgaacacggt
     5701 ccatacctgc ttctgctgca ggttctccac gccagagatt tcctccacca ataacaaggg
     5761 caatttcgat acctaagcta tgaacttctt gaatctcttt tgcgattgtt tgaactgttt
     5821 ggatatcaat ccctacgcca cgttcaccgg caagggcttc acctgataac ttgattaaaa
     5881 tacgtttata cttgggattc gccattttca ctctccttct ttcatcctac ctattttatc
     5941 acaatttcta agatttttat agtatcatga gcaattcttt caaaaaaatt agacagtcaa
     6001 aaattcctct aagtcggcaa gggcacgctc tgcaattttt tcataacgag ccttcttatc
     6061 acggatacgc tcgccttcca actccttgat gatcccaaaa ttgacattca ttggttggaa
     6121 atgtttgctg tcggcatggg taatgtaatg agctaagctt ccaatcgctg tcgtctcggg
     6181 gaaaataacc tcgctttctt ccttgaagag acgagctgcg ttaattcccg caactaagcc
     6241 cgaagccgcc gactcaacat agccttccac ccccgtcatc tgaccagcaa agaagagatt
     6301 tggttgtttc ttagaacggt aagtctgctc aagaagattt ggtgaatcca tgtaagaatt
     6361 gcgatgcatc acaccataac ggacaaactc cgcattttca agacccggaa tcatttggaa
     6421 gacacgcttt tgttctcccc atttgaggtg ggtctggaaa ccaacaatat tgtagaggct
     6481 accagctgca ttatcctgac gaagttgcac aaccgcataa ggtgttttaa attctccatc
     6541 acgaggtcct gtatagtcgt ctgggtactc aagaccgact ggcttcatag ggccataaag
     6601 catagtttta atgccacgtt tggccatgac ttcgataggc atacatcctt caaagtactt
     6661 ttctttttca aaagaattaa gcggtgcttc ttctgcattg accaaagctt catggaaatc
     6721 cataaattct tgcttggtca taggggcatt gaggtaggcc gcttctccct tatcataacg
     6781 tgatttgagg tagaccttgc tcatatcgat agtgttgaca tcgataatag gcgctgccgc
     6841 atcgtagaaa taaaaaccag caccgtcatt aagagcatga atcttttcag ctaaggcatc
     6901 acttgtcaaa ggaccagtag cgataaccgt aataacatct gtcggcaatt ctgtaatttc
     6961 atcacgaacc acttcaatca aggggtggtt ggcaactttt tcggtcacca tttgagagaa
     7021 accatcacgg tccactgcaa gggcaccacc tgcaggaaca cgtgtagcct cagcagattc
     7081 caagataaca gaacccaagc gacgcatttc ttccttgaga agaccaactg catttgtcaa
     7141 agcatcccca cgcaaagaat tggaacaaac caactcagca aaattgtctg ttttatgctg
     7201 gggtgtagac ttgacaccac gcatttcata tagtttaact ggaataccac gctctgcgat
     7261 ttggtaagct gcttcagaac ctgccaaacc agcaccgata acattgatat aagattgaga
     7321 cacgacacta atacctcttt gggagtgtga agttaagatt cacattgaaa aagccaatca
     7381 gacttacgag ctttcgagtt tcttggctca ggctgaaaaa gtccacagga ctttttcact
     7441 cccacaaatc tttctatttt ttcttctact agtataacaa aaaaagggaa gaaggcaaac
     7501 ttccctgttt agtcattttc ttgatgtaaa gagatagtga gtattccagt taagaatcaa
     7561 ttatatgcta ctctataaaa tcttttccac ataacggatc gatagggact gttattctat
     7621 ctcttgctat aaccgtatta tctaaaacag cataacattc aacatagtga tctcctttaa
     7681 actgtgaatc ttcgtgatat tttattttta cctgaaaaaa tagcaccacg ctcacaattt
     7741 cttctaatag cctcagcttc aatatttctt actttccaat agacttcctg cgaaacaaaa
     7801 tatggtatag tagttctatg aatgatgaag caagtaaaca actaactgat gcacgattta
     7861 agcgtcttgt tggtgttcag cgtaccactt ttgaagagat gttagctgta ttaaaaacag
     7921 cttatcaact taaacacgca aaaggtggac gaaaacctaa attaagccta gaagaccttc
     7981 ttatggccac tcttcactat gtgcgagaat atcgaactta tgaagaaatt gcggctgatt
     8041 ttggtatcca cgaaagcaac ttactccgtc ggagccaatg ggttgaagta actcttgttc
     8101 aaagtggttt tacgatttca agaactcctc tcagttctga ggacacggta atgattgatg
     8161 cgacgaaagt aaaaatcaat cgccctaaaa aaagaattag cgaatgattc tggtaaaaag
     8221 aaatgccacg ctatgaagtc tcaggcgatt gtcacaagtc aagggagaat tgtttctttg
     8281 gatatcactg tgaactattg tcatgatatg aagttgttca aaatgagtcg cagaaatatc
     8341 ggacaagctg gtaaaatctt ggctgacagt ggttatcaag ggctcatgaa gatatatcct
     8401 caagcacaaa ctccacgtaa atccagcaaa ctcaagccgc taatagctga agataaagcc
     8461 tataaccatg cgctatctaa ggagagaagc aaggttgaga acatctttgc caaagtaaaa
     8521 acgtttaaaa tgttttcaac aacctatcga aatcatcgta aacgcttcgg attacgaatg
     8581 aatttgagtg ctggtattat caatcatgaa ctaggattct agttttgcag gaagtctaat
     8641 gtaattctca agctgtgagt tcattatgat ttgtaaatcc gatatatctt cagcagctaa
     8701 aaacacattt tgaaatgttc caagtccgtt ttcaccaaaa ctatattctc tcttactata
     8761 cttactctta ttattctcta tatagttaga gtcaaggttc ttaacctctt gaagttcatt
     8821 ttctttactt ctgaggttaa aatcttttac ttctgaagtt aagttttttg acttctgtaa
     8881 gtcattttct tttacctgaa atatactcat aaagtcttta acataaatga tattcggctt
     8941 accaagtcca agccttactc tttcgatcag tcctattcct ttttttacat caagctcatc
     9001 taatgttttt atggcagttg gctttgagat atttcttctt ttcataattt cttcgacagt
     9061 aaaatagata aatactctgc cttccttgtc tatccagtta ttcttaaatg acattcctgt
     9121 ctgtttaaga agtatggaat aaaggataat tgcttctgct gataagcccc gaaattcttc
     9181 tctatcaact aatatctccg gtacttttaa gaagttaaat ctttccgctt ctctgttata
     9241 aaaatattca aagtccatgc aagatcacct cccttcttaa aaatttgcaa agaaaaagac
     9301 gatagttttt cttctatcgc ctttggtaac catttttatg aaatttttta aagttaaaaa
     9361 tataatttat aatactatct tcttccattt tctgatttta gttctgaaag ttccaaaagg
     9421 tgcaaccgta tttacatgaa taaacttata cacttcccaa gtcgctgttt tagtcgcttc
     9481 atcagcccac tttctcatat gcggttcaaa taattcttcc tcactcaacg aatcaatcat
     9541 tgcagagata gaattaatat tttcatttaa ttttgctttc aactcttgca gagataaatg
     9601 agcgtaggta tctgtgaacc actgatataa ctcaccaagt tgattccatt taaatttatc
     9661 cgatggtgtt tttacttgaa gcccctttct ttcatcttct tcccatttaa gaaccaagtt
     9721 ggtccaacca acctgataag caaggttttc tgctggagtt ctgtcaactt catcagctct
     9781 cttatctttt aaattttctg gaatattatc aaattctaaa atatatttct caaatgtttt
     9841 ctctatctca gcttttagtt cttctttatt ttcgtatgtt cgcaaaaaat caccttcttt
     9901 ctgatttatc aatcctttga tttaaaatga ccttattttt ctatttatac cttctaagaa
     9961 cgctataact gctgctccaa ccatcggtat tccgtatggg cttaataaaa aacttaatac
    10021 caaagcttct ataccaagtg atacatcttt atattatcat cactaattaa gttaaacaga
    10081 aacagacaga attattacat cctgtctccc tttattgttt ttcttcccag tcgtggtagg
    10141 cagcgtagag ttgactctta ttccactggt aaatcttagc tacttcctta atagcttgat
    10201 ttttcttcat accttgctgg atacgagctt ggatttctaa gaacaagtct tcctcgtctt
    10261 tttcttccac acctttgctg gcaccttcaa caatcagaag acattcaccc ttgagagacg
    10321 tttcagagat gctttccagc aattcagaaa ttgtacctct ttggtattct tcatagattt
    10381 tggtcaattc cctgaccaag acaacggagc ggtcaccgta gacttctaac atattttcca
    10441 acgtgtctgc tacacgatga ggtgattcat aaaaaatctg tgtttcagga taatcttttt
    10501 tcgagtcaaa aaattgcttc tgctggcctg attttctcgg taaaaagccg taaaagatat
    10561 gtggctgcgg cgctaaacca ctggcaatca aggcagaaat tcctgcagag gcacctggaa
    10621 ctgtaacaac tgcaatttct tcctcaatag ctgccttaac taaatcatga ccagggtctg
    10681 aaatgctagg caaaccggca tcagagacct gagcaatact ttgccctgct ttcaagaaac
    10741 caatcaaatc aggaattttt tccttggcat tgtgctcatg aaaactgatc tgcttggtgg
    10801 aaatgtcaaa atgcttgagc aaaagccctg tattgcgcgt atcctcagca gcaatccaat
    10861 ctacttcttt caaggtctgg atagcacgaa aagtcatatc atctagattg ccaatcggcg
    10921 ttgccactag atacagcttg ccatagggag actgcccctt aaaacttttt tgaatttgca
    10981 tgcctactcc ctgtataaca actcatcaca gaacatacat tcttcgtcct gctctcgacg
    11041 ttgtccataa aaatcattac atacgtgaaa tccatcacgg taaatacgac ggacactttc
    11101 gcgaacatgc ttggccttga cgggagcgtc tgcttccacc tcacccaagc gttctcgcaa
    11161 cttactattt tccaagcgaa gagctgtatt ttcttctacc aagctcttga gatttttctt
    11221 gatggcttcc acatcggcta aggttaccaa taattgttgg gaaaaatcat ccagcgcgtc
    11281 aaataattct tttttgtcca taaaacagcc ctttcctttc tttatcattc atttttgagt
    11341 ttatatttct ttcaagacca gatattccat ggcattttga aagctgacat tagcttgcca
    11401 catttttcta gcttctagta aatcttgtag aatcactctt actcttacct gcaagaggtc
    11461 ctgcccacag agaacttcaa gaatccgtaa aacctgatcc tgtttttcct tatcatctgc
    11521 caagttggct aatttggcaa cctgtagata actttctttt ttcttagcta ctaaccaagt
    11581 cagcaggcgt tcactttcat cgaccaaggt ccaaaaactt gcctgattag ccaacttttc
    11641 tgcttcagct cgcgattgac taaacttagt taaaagagtc gcttttttct taacaagtcc
    11701 catttgttct aagagtaaga tgagtttttc ttcttgcttt ttaaagtgga agatctgagt
    11761 ccgacttcgg attgtcggta acatcttttc ctcatcgcta gtcaagaaga aaatataaac
    11821 ttcactctgg ggttcttcga tgaccttgag cagagaattg gctgcgttgg gatgcatttt
    11881 atccgcttgc tcgatgataa agacctgttg ctggctttca atccctgctt gagaaaactg
    11941 tcccaccaat tcccgaatgc gttctgtctt gatgacctga ttgactggct taatcaaggt
    12001 gacatctgga aactcttcct gttcaatcag cttgcaactt cggcatttct cacatggtaa
    12061 gacgccaact ttatccgtac aaaagaggct cttagctaaa aattgcgcca tttccaagct
    12121 tccaaagaaa cctgaaaaga gataggcgtg attgagctgg tcttgttcta agatacggac
    12181 aaaacggtca aactgagctg gttgccaagc ctttagttga tcttgtttca tttggccaag
    12241 cccattccgt caaacaagac agccttggta gtttccacaa cttgctccaa agggagacta
    12301 gcatcaatct tgacaatgcg atttccttct ttttccagga gagaaaggta gccttgacga
    12361 acttttttat gcaagtccaa cccttccaaa tccaaacgat taacctcgcg gtcactatta
    12421 gcagcaatac gagccagccc ttcttccacc tcgatgtcaa aatagagtgt caaatcgggt
    12481 ttgaggccat ctgtcgcaaa ctgattgagc cagtcaatgg cttcaatatc taagccacga
    12541 ccaaatccct gataggcaac agaactatcg ataaaacgat ccatgatgac caacttgcca
    12601 gcttcaaggg ctggaagaac tttttccacc aaatgctgtc tgcgactggc aatatagaga
    12661 agtagctctg ttttagcatc catctgagta tgacttggat ccaaaatcac ttcccgaatc
    12721 ttctccccaa tcaagactcc gccaggttca cgggtcgtca gcacctttac tcctttttcc
    12781 tctaaaattg gtagcagagc ctctaaaaca ctggtcttgc ctgctccctc tggtccctca
    12841 agagagacta aaaatccttt tgacatgtct aactcatttc ttttttacta cttctattct
    12901 atcaaaaaaa taggggtttg tgacaatctt tttgtgtctt ctcatttcat actcaatgaa
    12961 aatcaaagag caaactagga agctagccgc aggctgtact tgagtacggc aaggtgaagc
    13021 tgacgcagtt tgaatttgat tttcgaagag tatcaaccac cataaaagag gcagataaaa
    13081 ctacctctta tttacctagt tcttgtgttc gtttgtaggc ttgatgaact gcatccatga
    13141 ctgttcctcg gaaagcatgc gcttctaggc ttgctacacc agcgatagtc gaaccgcctg
    13201 ggctacagac ttggtctttc aatactccag gatgttgctg gctttctagg accaattgcc
    13261 cagctcctac cacagtttgg gctgccattt tcaaggctgt ttctcgtggt aatcccgtct
    13321 gaacacctgc atctgccaag gcctcaataa agagatagac aaaggctggt ccacaacctg
    13381 caagacctgt cgctgcattg attaagcttt ctcctaattc aaccaagaga ccagccttgg
    13441 ctaaaagctg acaaaagagc tcactgtcct cagccctgca attaggagac aaggcataac
    13501 taatcactcc ttgcccgata gaagcagggg tattaggcat catacgaata attcggtgtt
    13561 ggcttgggat aagacttgct agtttttcta aggtcaatcc agctgccatc gaaatcaaaa
    13621 gaagactttc tcttttttca aggatggtct ggtattgaga aagcagttca gaaaactgag
    13681 caggcttaac acctagaaaa atcacatctg cttcttcgaa tatttcttca ttgttagaag
    13741 tctgaccacc gaagttagcg atgaaagcat ccaccttgac ttgactacga ttggcaagga
    13801 gaatctcatc tgacggtcta gcctgcaaga cagacttggc taaactagca cccatattcc
    13861 ccaaaccgat aaatccaatc ttcatccctt actcccttat ctgtccatca ccagtgacca
    13921 catacttgta gctagtcaat tccttcaagc ccatgggacc acgcgcgtgc aatttctgag
    13981 tagaaatccc catttcacaa ccaagaccaa attgtcctcc atctgtgaaa cgagttgagg
    14041 cattaacata caccgctgca gagtccactt gatctgtaaa gtatgctgca gcttcagcat
    14101 tttccgtcac aatagcatcc gaatgatggg tgctgtggga ttcaatgtgc gcaaccgctt
    14161 cttctaaact gctcacaacc ttaacagcaa ggacatagtc taaaaactcg gtgtcaaagt
    14221 cttgggtctc agctgcttga cctgaaacaa actggcttgc tttgctatcc aagcggaatt
    14281 gaattggttc cagtccagct tccttacgct ctgcaaccag catttgatcc aagcgaggaa
    14341 ggatgcttgc tgccttgttt tcatgaacca gcagaacctc catggcatta caaacagaag
    14401 gacgactggt tttagcattg ttgatgatag acagcgcctt gtcttcgtct gcatccttat
    14461 ccacatagac atggacaatc ccagtccctg tctcgataac aggtacaatc gcattctcaa
    14521 ccactgcatt gatcaagcca gctcctccac gaggaatgag aaggtctaga tagcccttgg
    14581 ccttcatcat agcataacta ctttcacggc tagtatcctc caccagttga atcacatttg
    14641 gatgaatagt agtcgtctcc aagcccttct tcaaggctgt gacaatggca tgggttgttt
    14701 gataggcatc cttaccacta cgaagaacaa ccgcatttcc actcttaaga gtcaaagcag
    14761 ccgcatcaga cgtcacattt ggacggcttt catagataat accgatgaca cccatagcta
    14821 cacgtttttt tgtgataacc aaaccatttt caagctgact tgtttctaaa acttcaccga
    14881 ttggatctgg taaggcaacc acttcacgaa ttcctcttgc catcgcttct atacgatctg
    14941 catccaaata aagacgatcc aacatcacat ctgagatttt ccccttagcc gctgccatat
    15001 cgagggcatt agccgctaaa atttcctcag tagcagccac taagtgatca gccatggcta
    15061 gcaaggcttg gtttttcact tcttcactag ctgtgttaat cgatttttta acagcctgta
    15121 cctgttcaaa ttgttcttgt ctactcacca tagtttacct ctaaaattct gtaaaaagta
    15181 gttggatttc aggagtaatg gaaatccagt cgtcacggta aatcaagaca cccttggctt
    15241 tttgagaacg caacatatcc tccaaagcag atgctccaaa ttgcacgcgt ccttttccaa
    15301 gtgattttcc actttcctta tcaaatactg tcacgatatc accgtaagaa aagactcctt
    15361 ctgcttcaac gataccagat aagagaagac tctttccatg ttgagagaga gcttccgcag
    15421 cccctttatc aacccaaata gaaccttgac tctgagcata gaaggcaagc cattgtttct
    15481 gggtacgaag ccccttctct tgagcaacaa agtaagaacc atcctcggtc tcctctgccg
    15541 cctcaatcat ggaatctgat ttcaaggatg agcagatata aacaggaact cctgattccg
    15601 tcgcgatagt tgcagccttg attttggtta acataccccc agttccgttt gacgaaccag
    15661 ctccaccagc catatcaata atctcacgat tgatggtctc gattctctcc aagcgtttgg
    15721 ctcttggatc tgaattagga tttccagtat agagaccgtc cacatctgtc aagaaaacta
    15781 aaaggtctgc ttggaccatc gccgctactt gagcacttag agtgtcattg tccccaacct
    15841 tgagctcatc aataacgaca ctatcattct cattgatgat aggaattgcc ccacggttga
    15901 gcaaaaccga caaagcctga tgggcatttt tataacgacg cttatccaca aagtcatctt
    15961 gggtcagcaa gatttgtgca gaaacgattt gacgcaagag aagattggtt gtatattctt
    16021 ccaacaaaag cccctgccct accgctgctg aagcctgttt atcagcaatt ttagtcggac
    16081 gctttttaaa tcctaaggct ccaaaaccag ccgcaatggc acctgaagac accaaaatca
    16141 actcatgacc agcctcgtgc agcatagcca actgctgggt aatatccttt accttactac
    16201 gtgataaact tccatcctca tttgtcagag aagaagtacc caccttaaag acaatccgtt
    16261 tgtatttcat agtctcactc cgattattca tatttatcca ttataacatg aataacagga
    16321 aatagaaaag agttgatagg aaaaagaagg ccctaaaacc ttcttttcta ctactgttct
    16381 gattccgatt tgtttacact cgtttcagag ctttgtgaag ttgaggatgt caaatcttga
    16441 ctagttgaag aatttgtaac actttcatgt tgactcgttt ctttattttc aagatccttt
    16501 ttcactactt ctttggattc ttttatttct gaatgacttg tagttttaat tgtagcagat
    16561 ttctcgatcg gaacatccac taaccaggca ccacttgaat ccacagtata gccttctggt
    16621 gtcttaccat tcacaagtaa ttgaccattt tctttcaaga agtaccattt atccttgtct
    16681 ttaatccaac ctatagctcg tgaaccatct gtcttgtaga aataccaatc atttgccttt
    16741 ttaagccagc cttgcttcat agctcctgat tcttctagat agtaatgatg tccagcaact
    16801 tctatctcac ctgtcttcat gataccagta gaatccatat agtaccaggc ttctttatct
    16861 tttacccagc ccgttttcat ttcccctgat ggagcaaagt aataccattg ccccttatat
    16921 tgaagccaac ctgtcttcat agagccatca acatccaaat aataccactg ctctttatct
    16981 tgaatccaac caatttccat ctggttttct ttgttaaaat aataccagat tccagcgatt
    17041 ttcttccaat ttttagcagc aacaccagag tctgtcaaat agaaccaacg attgttccat
    17101 tttttccatt gattctgtaa taagataccc gtttggttaa agtaatacca ttcaccctta
    17161 atttcattcc aaccaacagc atattctcct gtagaatcag gcgcttgata ccaccagttt
    17221 ccatatgaac tcttatgcca accagcttga aaacttggaa tcggcttgta ggaagttgaa
    17281 atgtttacta aaccgtcttg tcgaatatca aaaactgttg catcatagtc tttgctggct
    17341 gcgttgattc tctcaattcc tcgttctttg agccaattaa catactcact atcaacacca
    17401 tttttccaag gtagactatc cgaagtttga acaatcaaac tcggactcaa atttttaatg
    17461 aaatccttgg tatttgattt gttggtatca tggtgatgat taaacttcat caaatcaact
    17521 tttccaatga gaggaccata cttgtcttct gctccatgaa cattatctaa atcgccccca
    17581 aggtaaattt tcttgccatt gactttcacc acgctaatca aggaattgga attgtcatcc
    17641 caaattttct ttaattcacc cgatgaatca gtttcatttt cataattata gagctgaata
    17701 tccatgtccc caaactgaaa atgagcatcc ccttgtgtga tattttgaat aactgaaaca
    17761 cctttttctg cagcagtctg taaaacctta tcatagccat acagattatc ccatagacgt
    17821 tcagaattag taatacgact atcactatat ttcttaagat agactcggtc aactggatag
    17881 gtagacagta attcatcaac atttccaata tgatcactgt gggtatgggt caccaaaata
    17941 aaatcaagtt tttggacacc caattccttc aaacgacgaa agacacggtc tgtcagaaca
    18001 tgcttataag tggtattaat accctgtctc catggatagc gagaatcact tccatctggg
    18061 aaatcataat cttctcctgt atccaccatg gcaaaatgtc cattgctttc aagaataatc
    18121 gcatcactgc caccttcttg aacattgata aagtggattt tatttcctga actttcttga
    18181 gcctgaacat tcccatacca tgacaaacct aaaaaagcgc ctacaagtgc taaactagtt
    18241 aatttctttt tcattcttac tttctcaatc tctccaaggt ttccttaaaa atctctggga
    18301 tatctgcttt aaattccaag gtcttacctg ttctcggatg agtaaaacct aaagtcttgg
    18361 catgaagaaa ttgtccatgt cctttcaaag tcttgcgagg accatagacc tcatcaccag
    18421 cgactggatg gccgatataa gccatgtgga cacggatttg atgagtacgc cctgtctcca
    18481 gttgcaactc tactaagcta taatcgccaa agcgttccaa gacgtgaaaa cgcgtcactg
    18541 caggcttccc tttagcagtt acagcctgtt tcttacggtc tttttcactc cggccaatcg
    18601 gcgcttcaat tacaccacga tcattaggca gatttccatg aacaatcgcc caatatttgc
    18661 ggagagactt tttatccttg agttcttggg caagtgctag atgcgcatca tcgtttttag
    18721 caatcatgag aagacctgac gtatccttat caatacggtg aacaatccct ggacgcagaa
    18781 ccccattgat acccgacaag tccttaatat gatacatgag ggcatttact agggttccac
    18841 tggtatgacc agcactcggg tgcacaacca ttccctgagg tttgttaacg acagccacat
    18901 cctcatcttg gtagactatt tctagcggaa gatcctcagc cacatactct aatacctctg
    18961 gttctggcac atggtaagtg acgacatcac cctcttggac tgtgtattta gctttcttga
    19021 cttgaccatt gaccaagacc tggcctgatt taatttgttc attcgcgaga ctacgtgata
    19081 attctgacaa atctgacaaa gccttatcca aacgcagacc accagtttca attttaattt
    19141 ccatttattt cctcttttag cattgcaatc aataaaataa tcactccaac cgtcagatag
    19201 ctatctgcca cattgaaaat tgcaaagttg ataaagtcaa ggtggaacat atccacaaca
    19261 aagccctgac tgaccctgtc aataaagttt ccaagaccac ccgcgattat tagagtcaaa
    19321 cccaagacca tccagaatga gtcctccatg tgtttatgta aataccaaat ggcacctatc
    19381 acgacaacca gagtaatgac agcgaataac aactgctgat cttgtaagat agaaaaggct
    19441 gcacctcgat tttgcaggta ggtcaagcta acgaaattgg ggatccagga gcgcacttca
    19501 cccagtggaa tctgctggac gatataggat ttgaccaact gatccagccc aattaaaagc
    19561 agtacaatga ctgccactat tgctcttttt ttcatgattt cctcttttga tcaaaatatt
    19621 cttgcatgac ttctacgaag agagtcccag cttgactaag ctccacttct tcacgtttaa
    19681 catagaccat gcggttatct aggttatcct tgagacgaat aactgtaatg ccattaacac
    19741 tgtcactatc taaaaatcca gaacctgtcg cataggcgtc cgtccgctcc aaaataccat
    19801 tcaaggtggc acggtctgtc acattaaaca tctgtgagct agcgctggta tcgacaaagt
    19861 tctctgaata ataaaggtac tcgtctttct cttgagtgaa acgaaccgtt ggtaaatccg
    19921 ctaaatcctc catgactaat tcatctttct gggctaaagg atgaccctca cggagataaa
    19981 tatgggtatg gaaaggaatc aattcgatga cctccagacc taatttttca acccgttgca
    20041 taatcccctt tttattttga ttgttgaggt agataatccc aatctcacta tgcccttgcg
    20101 ccacttcatc taatatttga acagtagttg attcaaaaat acggaagttc ttatagtcag
    20161 gatagcgctc tgaaaaggcc gtaatagttg gtggtaagaa gtcatagtgc tggctagcaa
    20221 cggaaaattc atctttttct tcttcaggat tggcatactg attttgaaaa atatcaaatc
    20281 ctttaaccaa ttcttgcgct ttttcataaa attccatccc acgacgggtc aagaaagtcc
    20341 ctgagctggt ccgacggaaa atcttaaaac ccaactcttt ttccaaatcc cgaacagaaa
    20401 tagacagact cggctgacta acatacatct tttcagcagc ttcacgaaaa gtaccactat
    20461 tggcaatagc cacaacatag cgtaattgtt gaatgttcat cttctacccc taacttcttt
    20521 atcttttcat tataccatat tttagaagtt ttccaaaaag gaaaaaagaa catcctattc
    20581 ttcttaacta tcttcactat ctgcctttct caagccaacc ttattttcaa aatcaattca
    20641 aaaaaataac tggctacaca ccactacaat atcgaaaaga aaaacgttga ctatttgtga
    20701 aaaaagaaaa tgccggaaag ttccgacatt attttagtaa gctaacttcc tgaaaatagg
    20761 agcaaccaca catcccagaa ggcagtgata ttaatgagaa tatgaactag tattggatag
    20821 tagagatttt tagtcatgcg atacaataga gctaaaataa gacctccact agcataaatg
    20881 aaaaaagcaa gaggagttaa agcaaaattg atatgaatat aaccgaaaat aatagcagaa
    20941 agcaaaacat ctccgtacca aggcgagttt ttgaaaaagg ttgtcataat cacaccgcga
    21001 taaatcaatt cctcagcaat aggggcgatg aagcaaacga tgagcaagaa ataagggaac
    21061 tcctgtcgtc ccatcatttc tatcgtttca ttcaaagaaa tttgatttga aggcggggat
    21121 atgaaatacg aaaagaggaa gtcagacata tatgaaatga tgtagcccag taaaaggtag
    21181 atgaagtacc tcagctgcca cttgaaatga aaaattttct ttcctgcaaa aaccagtaga
    21241 tagatgaccg ctaagaaaag aactccactc tccatcaaaa gaagaatctg aaaaaattca
    21301 cgacttgctg gtagataggg ctttgcgaga taattgaaaa gcctccaaaa ccaagttgat
    21361 ttgtaaaaaa ttaaggacaa tactagtaaa atttgaatag ctcgtttttt catattaaat
    21421 cctctgtttt ctccccttgt ttcttcatat tcctaataaa ttttacttaa aatctcgcag
    21481 cacttccttt gcaaagatga ttacctcctc caatatatcc taaatcatcg gttcctctgg
    21541 gacaaaatcc atgaaccagc agagttaata ctggttctat ttcccaatcg tacctaccat
    21601 taggattttt accatatgga tctgctccac cttgtatcag ctctaattct tcatttgtta
    21661 gatttatatt ttttgtcatt aaaatcatga tatatttcct ccattattct tcagattagt
    21721 tgagtaatct gctactcata cctacttaca aaaaaactat tatattaagt tggtttttta
    21781 atttgtctag ttgcaacatt gacaaacata gtatagcata tctttgcgaa atatcctctt
    21841 caaatcatga attgtcatca aaacatctta aactataaaa tcaattagtc tcaagctttc
    21901 tatcaatttc ttctccaaaa tatgctataa taatagcaaa agataaagaa ggaagaccta
    21961 tgattaaact actagccttg gatatggacg gcaccctcct caatgaagcc aaggaaatcc
    22021 cacaagctca cattactgct attcacaaag ccattgaaaa aggtgtcaaa ctggttctct
    22081 gtacgggtcg cccccttttc ggtgtcctcc cctactacaa aaaactggag cttgacctcc
    22141 agaacgaata tattattgtt aacaacggtt gttcaactca ccagactagc gactggggct
    22201 tggttgactg gcaagaactg agtccagctg acatcgaata cctctatgac cttgctgaaa
    22261 agagtgatgt tcagttgaca ctttttgacg agtcacatta ttttgttctc ggtggcaaac
    22321 ccaatcaagt tattgaaaat gatgctaaac tagtattttc agacctgact gaaatttctc
    22381 ttgaagaagc gactagtgga aagttccgga tgttccaagg tatgttttta ggaacaaaag
    22441 aacaaacaga cgattttgag cagcgttttg ctgaagagct ttgccaacga ttcagtggag
    22501 ttcgttcgca gcctgtcatt tatgaagcaa tgccgcttgg aacgacaaag gctactgctc
    22561 tttcacgact agctgagatt ttgaagattg attcctcaga gattatggcc atgggcgatg
    22621 ctaataacga tatcgaaatg ctccagtttg cagggcttgg gattgcaatg ggaaatgcca
    22681 gcgattatgt caaatctctt gcggatgccg ttacatcaag caacgaagaa gacggcgttg
    22741 cgcgtgctat tgagaaatat attctataaa aaagaaaagc aaatagacag aagttactgc
    22801 tatttgcttt tttgctaata ttttaaaata cggactaatc aatagacaag aatagcgaat
    22861 cataaaaatc gatttactag atgccatagt atcttatagt tgctaataag aagttagtct
    22921 agcagaatga atctctcatt tctcccacac taatctgtaa tttgtctata catttctggt
    22981 cttctatctc gaaacaagcc ccagtttagg cgttcacttg ctcccttgtc taagtcataa
    23041 gtagctaaca gaacagcttc ttcttgtctt tcagctcgtt ctagaatagc tcctgtttca
    23101 tccgtcataa aggaggaacc gtagaagtca agactggaac tctgtccgcc attttcctca
    23161 ctaggagtaa cctcctctaa accataacga ttggctgcga tgactggaac aatattcgct
    23221 gctgcgtgcc cttgcatagt acgttgccag tgaccacaac tatctgtatc caaaattggc
    23281 tctgaaccga tagctgtagg ataaaagaac aattcagcac cattcaatgc aagacagcgc
    23341 gctgtttcag ggaaccattg atcccaacag ataccgatac caatcttagc atagcgagta
    23401 ttccagacct tgaaaccagt gttaccaggc gtgaaataga atttttcttg ataataatgg
    23461 tcatctggta tatgggtctt tcgataaacg cccagcactt ccccatctgc atcaatgacg
    23521 gcaatagagt tatacaagac attaccatct ttttcataga aactgattgg taaaacaact
    23581 tgtagttcct tagcaatcac cttaaaatgc tgaatggcag tattttccgc tacagattgg
    23641 gcatactggt agtagtcata ctgacgttcc tgacagaaat agggatgttc aaacaactcg
    23701 ggcaagagaa taatttgggc tccttgctca gcagcctgac gtactaaacg ctctgcggtt
    23761 tggatatttg ttgccacatc cttagcgcat tgcatctgaa tggttgcaac tcttacattt
    23821 ctcatctttt tctcctattc tggaatttgt tgggtgatac agtggatatt gccaccacct
    23881 aagagaatat ctctggctgg tattccgaca actttacggt ctgggaaaca cttgctgagg
    23941 atatctaagg ccacttggtc gtttacatcc tcaaactgtg gaaccaagac agccttgttg
    24001 gcgatataaa agtttacgta ggaagctgct agtcgttcac ctgcgtatcg cttttcttct
    24061 ccttcttcat agatgtagcc tggcaaatct tcttctgtca caacttgtcg aactgcaggg
    24121 ataggcaatt tatgaatggt gaagtgacaa ccttttgcat ctgtttcctg ttctaagagt
    24181 tcgagatctg cttttgacat ggcatactgg ggatcatttt cgtcatctgt ccaagccaaa
    24241 acaagctcag caggaccaac aaaggcagca acattatcga cgtgttcatt ggtttcatcc
    24301 tgataaatac cataaggaag ccaaataact ttttcagcac caagactttc taataatgtg
    24361 ttttcaatct cctctttagt caggttagga ttgcgaccag gactaagcaa gcaactttca
    24421 gttacgagaa tagttccttg accatcgcta tggattgcgc ctccttccag tacaaaaggt
    24481 ttagcatcat agacaggcct ttccaaggcc tcagcaaaac gactggctac ttggtcatcc
    24541 tcttcataat cttgataaag accatcatag gtgcctcccc aagcattgaa ggcccaatcc
    24601 acggctaatt tcttaccttt atcattgacg agaatggttg ggccagtatc acgcgcccag
    24661 gcatcattgg tgggaatgtc taaataaaca accttgtctc caagatagga ttgggcttca
    24721 gatagatagg cctgctccac caaaagatag actctttccc cttctgctat ggtctcgatg
    24781 atctgagtaa atgctctttt agcagccttt ccttgaaaag gccatgatcc tggtcgagtc
    24841 ggccatatca tgagggtacc atgatggggt tcgtactctg ctggcatgtg atagcctaat
    24901 ttttttggac tgtccatcat gataatctcc ctttaaagtc ttgatagcca aaagctttga
    24961 gtaagctaca gtctccctgt tcgtccatga gatagagact tggcaatcca ataccattaa
    25021 aggtattatt tttgacaaaa gaataaatgg ccatgtcttg aaaataaagt ctgtctccga
    25081 tttggactgg attttcaaaa ctataatcac caatcacatc gcccgtcaga caggtattag
    25141 aagaaagtct gtaggtatgg gctttttcct gtgcctcaaa gccatttctc aaaggtggac
    25201 gatagggcat ctcaagtaca tcaggcatat ggcaggtcgc agaggcgtct aaaaccaaga
    25261 tttccatacc gttttctaca atatctaata cctcagttgc taaataaccc gcattaagcg
    25321 caatggcttc accaggctcg atatagattt caagattgta agtttttcgg atacgcttga
    25381 tttctgaaat cagcaaatcc acatcgtaac cttctcttgt aatatgatga ccaccaccca
    25441 tattgagcca ttttacctca tgtaagtagg gaccaaactg ttcttctact tctttcaaag
    25501 ttgtttgtaa atcatctgct ccctgctcgc aaagggtatg aaaatgaagt ccgtcaacca
    25561 aatctagcaa atcactcgga atcttgtcta tagtaactcc aaagcgagaa cctggtgcac
    25621 aagggtcata gagcgcgtga tctccttgag ttgaacactg agggttgagg cgcaaaccaa
    25681 cactgacacc agcctctcga caacgcgaac catgcttacg caactgtctc tctgagttaa
    25741 agacgatatg gtctgttatc ccaagcaatt cctccatgtc ctcatccttg aaagctggtg
    25801 caaaaacatg gacttcacca ggaaattctt cccttgccaa tttggcctca tagagtccac
    25861 tagctgtcgt acctgataga tactggctaa tcaagggata agttttgtag agggaatatg
    25921 ccttctgggc aagcaagacc ttgcaaccgg cctcttcttg tacatattgt agaatgcggc
    25981 aattagcttc taacttggcc aagtcaataa cataggctgg tgttggtact tgttctaact
    26041 tcattagtcc accatttgtg gattttcaac cacaacccat ggcaaaccat actcattcaa
    26101 agcttccatg aatggatctg gatctaactc ctcaaggtta tacactccag cttgtttcca
    26161 agttccgttc atgactaatt ttgtcccaat catggctgga actcctgtcg tataagaaat
    26221 agcttgcgaa ccaacctctg catagcactc ttgatggtcg cagacattgt agatatagat
    26281 agtctttttg acaccgtctt tgacacctgt aaagatacat ccaatattgg ttttacctac
    26341 agtacgtggg ccaaggctag caggatctgg aagcaaggct ttcaaaaatt gaattggaac
    26401 aatttcttgg ccgttaaagt taatggtatc cgtacgaagg agtccaacat tttcaagaca
    26461 tttcatgtgc gtcaagtaag attgaccaaa agtcataaag aaacgaatgc gtttgacacc
    26521 tggaatgttc ttggccaatg attcgatttc ttcatggtga aggagataca tatctttttg
    26581 tccaacttga gggaaatcat actcacgctt gatagacata gcttcgactt cgacccattt
    26641 cccatcttcc cagtaagaac ctggcgcaga aacctcacgg agattaattt ctggattaaa
    26701 gttggttgca aatggataac cgtggtcacc gccattacag tctaaaatgt cgatataatg
    26761 gatttcatca aaatagtgtt tgagggcata agctgaaaag acactagtta cacctgggtc
    26821 aaaaccagaa ccaagaagag cagtcaagcc tgcttctttg aatttctctt gataagccca
    26881 ctgccatgag tagtcaaagt aggctgtaaa accaagttcc ttacaacgtt tttcgtagat
    26941 agcacgccac tcagggtctt ctgtgtcttc tgcttcgtag ttggctgtat cgatatagtg
    27001 aacacctgtt gccaaacaag catccataat ggttaaatct tgataaggca gagctacatt
    27061 caaaacagct tctggtttgt agctttcaat caaggtaatg acctcttcta ccttgtcagc
    27121 atcaagtgct gcagtttcaa ttttagtact tgttttgcct tctagcttcg ctttcaagtc
    27181 atcgcatttt gacttggtac ggctagcaat cataatctct gtaaatgttt cgctatcttg
    27241 acaaattttt gaaatagcaa cttgggcaac gcccccacaa ccaataacta gtaaacgact
    27301 catttttttc cttcctcttc ttctaaaatg tcctcaacat acttgggcaa cataaaggct
    27361 cccacgtgta agtttgcagt gtagtattct gtgaaaagct ggcgtttttt ccaaccttcc
    27421 ttgtcaaaat ctttgacagg gtggtatttt ttcgatgcaa atccaaacaa ccaatagcca
    27481 gctgggctag ttggaatatg ggcctgataa acccgactga ttggaaaggc ttgattgacc
    27541 ttgcggtgca tgcttcggca ggccgactca tcctcgtcaa agaagggact cccatgctgg
    27601 taaatcatga tgccgtcttc cttcagagct cgataactat tgccgtagaa ttccttggta
    27661 aagagtcctt ccgtatggcc aaatggatct gtcgcatcgt tgatgataat atcgtaatca
    27721 tcttcgcagt ttcgcaaaaa gcgtagccca ttttggtagt aaatggtaac acgaggatca
    27781 tctagccctg cagcaaagtc tgggaaatac tcacgacaga cctcgaccaa catctcatcc
    27841 ggttccacga tatcgatttg ttccagttca ggatagagtg ttaatacttg ggcaacaccg
    27901 ccgtcaccac ccccaataac caatactttc tttggatttg ggtggacagc catgggaacg
    27961 tgaacggtca tttcattgta gacgaaatca tccgcatctg agaacaagac atggccattt
    28021 aaaatcagta ttttcccaaa agctggcgta tccaagactt cgatatcctg ccattcactt
    28081 tttccagcgt aaagttgctt ggctgttctc agagacaatt tgacatctgg agtatgaact
    28141 tcagaaaacc ataaatccat ctatttctcc ttatattata tttagcgcag ttagctactg
    28201 ccaaagccca agtggtatac ttggaataag ccactgtgga ttagttcatt tcctccaatc
    28261 gcttgacgat ttccgaaagg gtctaaccac agctttttgt ttcaattggt aatgagtttg
    28321 ataacgcata gacgttttat ctcgtctaag gtaacaaggc aaaaagttcg tagataacta
    28381 agtagacttg aaatactata tctggaggta ctcccatgct ttatgtgggc attgatgtcg
    28441 ctaaaaataa acacgatgtt acagccttga atgtttcagg aaaactgttc ttaaaccact
    28501 cactttttca aataataaag ctggttttga actcttagat ctgtctcttc gacagctcaa
    28561 ccaagactgt ctcatcgctc ttgaagatac tggacattat gcctttaatc tactgaactt
    28621 cctgcatgaa caaggatata aagtttacac ctacaatcct ttgctcatca aggaatttgc
    28681 caaatcatta tcacttcgca agactaaaac ggataagaaa gacgctcatg gcatcgctct
    28741 taaacttctt tctgacccca atcgtgaaca atttcaacac gataatcggc aagtagacct
    28801 aaaaatactg gctagacata ttcatcgtct caagaaaaac agtctgattg gaaagtacaa
    28861 tacactcgtt gtcttgatat catctttcct gagttggata aaatcgttgg aaagcattca
    28921 gaatatacct accaactctt gacacgctac cctaatcctc agaaaaggat tgaggcagga
    28981 tttgataagc tgatagaaat taagcgattg accgcttcta attcaagggc tttttgccgt
    29041 gctctaaaat catcgccaaa tcatcaaatt gaaataagaa atagtttaga taaaactatt
    29101 gacttttctt atagtttgtc tttctcttaa tgacgttgat atgattgacc tctggatctt
    29161 ccgtcccttg gagggagcaa ccacgttcct tggcgaattg gatatagtcg acaatttctc
    29221 gtgtaatgcg ttcaccagga gccaagatag gaatacctgg agggtaacac ataacaaatt
    29281 ctccacagac ctgtccaaca gaatcatcca aagttaaact ttttctttct gaatagaagg
    29341 cttcttgcgg agacagcact aactcgggct gaatatattc tcctgctatc aaatcttttc
    29401 catctcttga atagagtctc ttaatatcag ccagagcacc aaccaagcgc tcgatgtctt
    29461 ggatgcggtc gccgatggaa atataggcca agatattgcc gatatcacca aactcgatct
    29521 gaatgtcgta ttcgtctcgc aagaggtcat aaacctcgat acctgttaag ccaataccct
    29581 gagtgtaaac tgacaactta gttacgtcaa aatcgcaaac cgaaacaccg tctattaact
    29641 cttttgagta ggcatagtag ccaccgatag cattgatttc acggcgggca tactcagata
    29701 gctcaatgac tttctcaaac gactctttac cacgaagggc caagttgcga cgtgaaatat
    29761 ccaaactagc catcaacaag taagaggcag atgtagactg ggtcaggtta attatctgac
    29821 gaacgtattc agaattcatc tgctccccga taagtaaaat ggagctttgg gtcaaactcc
    29881 caccagactt atgcatggaa actgctgcca tatcagcccc tgcatccata gcagaaattg
    29941 gaagtttatc agtgaaatgc aaatgcgctc cgtgggcttc atctactaaa accatcatgc
    30001 cagcttcatg agccatttct gtcaacccct ttaggtctga acagatgccg tagtaagtag
    30061 gattgttgat taggatagcc ttagcatctg gatggtcctt tatggcctgt gctactcggt
    30121 cattttcaag acctaaagcg ataccaatct taggatctac actcatctcg atatagatgg
    30181 gaatagcacc acatagaacc aacgcattga tagcagattt atggacattt cgtggcagaa
    30241 taatcttatc tcctgccttg caggttgcca gaatcatagt ctgcaccgat gaagttgttc
    30301 caccaatcat tagaaaggca tggctagctc caaaagcatc tgcagccagc tcctctgcat
    30361 cacgaataat cgaaatagga tggcctaaat tatccaaggg tttcatcgaa ttgacatcaa
    30421 tgcctacaca tttttctcct aagagttcga caagttctgg atttccccgt ccacgcttgt
    30481 gacctggaac atcaaaggga acaatccttt tcttgcgtaa cttcaccaag gcctcataaa
    30541 ttggggcttg gttttgatct aactctttca aaacatactc ctttcaatat ttttaggacc
    30601 ttatgaaaac taaggaacta aaggttgact catgaaaaaa gagcaggttt tcacctgctc
    30661 tgcaatcttc tgacgtgttt attttggttt ctgttgagta tgtctgttcc cgatgtttcc
    30721 taactctcta gtagcaaata ttacgtactt tattgatcgt ttcacaagat gacgtgtgct
    30781 ttcaccacac taaaaattta tgaattagga caagtgtcct aacatcaact tataaaagta
    30841 ggcttttaac cctatcaata atgtggcttt tcaaccacgc ttcggcattc aaccctgcct
    30901 gtccgtaggc attttttact cgggtttata tttgctagaa aacatcatct cattttctaa
    30961 gctttattac tatatcatgt ttttaagaac ttgtaaagta ttttttgata aaaa
//
LOCUS       DAVKGU010000018        27180 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKGU010000018 DAVKGU010000000
VERSION     DAVKGU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.018"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(93..347)
                     /locus_tag="VW077_000757"
     CDS             complement(93..347)
                     /locus_tag="VW077_000757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454816.1"
                     /translation="MKIKEQTKKLTAGCSKHCFELTSSVLSATSKRCFELTSSVLSAT
                     SKRCFELTLSVLSTTSKQCFEHHAASFLVCSLIFIEYKNR"
     gene            complement(375..2390)
                     /gene="recG"
                     /locus_tag="VW077_000758"
     CDS             complement(375..2390)
                     /gene="recG"
                     /locus_tag="VW077_000758"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9454817.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLQTIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKSIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFACKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(2409..3512)
                     /locus_tag="VW077_000759"
     CDS             complement(2409..3512)
                     /locus_tag="VW077_000759"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9454818.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(3502..3870)
                     /locus_tag="VW077_000760"
     CDS             complement(3502..3870)
                     /locus_tag="VW077_000760"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9454819.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(3909..4940)
                     /locus_tag="VW077_000761"
     CDS             complement(3909..4940)
                     /locus_tag="VW077_000761"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9454820.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(4942..5973)
                     /locus_tag="VW077_000762"
     CDS             complement(4942..5973)
                     /locus_tag="VW077_000762"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9454821.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(6054..8567)
                     /gene="secA"
                     /locus_tag="VW077_000763"
     CDS             complement(6054..8567)
                     /gene="secA"
                     /locus_tag="VW077_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9454822.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDIITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(8713..9000)
                     /locus_tag="VW077_000764"
     CDS             complement(8713..9000)
                     /locus_tag="VW077_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454823.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(9028..9660)
                     /locus_tag="VW077_000765"
     CDS             complement(9028..9660)
                     /locus_tag="VW077_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9454824.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKFDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(9664..10305)
                     /locus_tag="VW077_000766"
     CDS             complement(9664..10305)
                     /locus_tag="VW077_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454825.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMILYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKV"
     gene            complement(10298..10924)
                     /locus_tag="VW077_000767"
                     /pseudo
     CDS             complement(10298..10924)
                     /locus_tag="VW077_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10963..11777
                     /locus_tag="VW077_000768"
                     /pseudo
     CDS             10963..11777
                     /locus_tag="VW077_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
     gene            complement(11774..11962)
                     /locus_tag="VW077_000769"
     CDS             complement(11774..11962)
                     /locus_tag="VW077_000769"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454826.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLDLFRI"
     gene            complement(12147..12482)
                     /locus_tag="VW077_000770"
     CDS             complement(12147..12482)
                     /locus_tag="VW077_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454827.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYIPLY
                     ALFSLSWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(12622..12957)
                     /locus_tag="VW077_000771"
     CDS             complement(12622..12957)
                     /locus_tag="VW077_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454828.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWIYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13048..14358)
                     /gene="der"
                     /locus_tag="VW077_000772"
     CDS             complement(13048..14358)
                     /gene="der"
                     /locus_tag="VW077_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9454829.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPGMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14372..15085)
                     /locus_tag="VW077_000773"
     CDS             complement(14372..15085)
                     /locus_tag="VW077_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9454830.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLKQKKLL"
     gene            complement(15082..15978)
                     /gene="dnaI"
                     /locus_tag="VW077_000774"
     CDS             complement(15082..15978)
                     /gene="dnaI"
                     /locus_tag="VW077_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9454831.1"
                     /translation="MESVGDVLKRQPSRFHYQDLVQKIMKDPDVAAFVQQESLNQDEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVMNHGYADVSYEETPELIAAEKEAA
                     IKKRLNLINFPSSLKNVSFLDVYRDDVQRLTVLKRMIEFVNDYPNNLKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKGKNGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(15979..17148)
                     /locus_tag="VW077_000775"
     CDS             complement(15979..17148)
                     /locus_tag="VW077_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9454832.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPEYKNTSSAEELEEMERQTLELLAKLDNGGD"
     gene            complement(17149..17622)
                     /gene="nrdR"
                     /locus_tag="VW077_000776"
     CDS             complement(17149..17622)
                     /gene="nrdR"
                     /locus_tag="VW077_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9454833.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            17762..18127
                     /locus_tag="VW077_000777"
     CDS             17762..18127
                     /locus_tag="VW077_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9454834.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18132..18827
                     /locus_tag="VW077_000778"
     CDS             18132..18827
                     /locus_tag="VW077_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454835.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            18844..19611
                     /locus_tag="VW077_000779"
     CDS             18844..19611
                     /locus_tag="VW077_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454836.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(19964..21162)
                     /locus_tag="VW077_000780"
                     /pseudo
     CDS             complement(19964..21162)
                     /locus_tag="VW077_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(21159..22052)
                     /locus_tag="VW077_000781"
     CDS             complement(21159..22052)
                     /locus_tag="VW077_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454837.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22168..22248
                     /locus_tag="VW077_000782"
                     /pseudo
     CDS             22168..22248
                     /locus_tag="VW077_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            22733..22946
                     /locus_tag="VW077_000783"
                     /pseudo
     CDS             22733..22946
                     /locus_tag="VW077_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            23361..23978
                     /locus_tag="VW077_000784"
     CDS             23361..23978
                     /locus_tag="VW077_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9454838.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(24021..24908)
                     /locus_tag="VW077_000785"
     CDS             complement(24021..24908)
                     /locus_tag="VW077_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9454839.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(25057..26940)
                     /locus_tag="VW077_000786"
     CDS             complement(25057..26940)
                     /locus_tag="VW077_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9454840.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
BASE COUNT         8049 a         6030 c         5079 g         8022 t
ORIGIN      
        1 agattttagt ttcaaaaaat tcttttatat aataatgtcc ttgaaagtag tttcagctga
       61 acatttatag tccataaaaa gaaaacagaa actcatctgt ttttatactc aatgaaaatc
      121 aaagagcaaa ctaagaagct agccgcatga tgctcaaaac actgttttga ggttgtagat
      181 aagactgaca aagtcagctc aaaacaccgt tttgaggtgg cagatagaac tgacgaagtc
      241 agctcaaaac accgttttga ggtggcagat agaactgacg aagtcagctc aaaacagtgt
      301 tttgagcaac ctgcggttag cttcttagtt tgctctttga ttttcattga gtataagttt
      361 tccttagaga aagcttaatc cagatgttct ttcttttcca gatgaagggc aatcatgcgc
      421 cactctggat cttcttgcca agcttctata gaactaatgt agctagcaac ctttcttgct
      481 tcttctaaaa tcggaaaatc ttcgataata tcagccactt ggaactctgg aagtcctgac
      541 tgtctggttc caaaaatctc accagaacca cgcattttca aatcttcctc cgcaaggaca
      601 aatccattgg tcgtttctgt catgatgcgc atgcggtctt tcccagaatc cgtcttggga
      661 ttggcaacga gaacagcgta ggactgtttg tccccccgac cgacacgacc tctaagctgg
      721 tgaagttgac tgagaccgaa gcgatcggca tccatgataa tcatgacagt cgcattggga
      781 acgttgaccc caacctcaat aaccgtcgtc gaaaccagaa tatccgtctt tctctccttg
      841 aaatcctgca tgatctggtc tttttcgtca ctcttcatcc taccatgtag aagagccacc
      901 tctgccttgc atgcaaaatg agtcgtcaac tcctctgata aggcaatggc atttttcaaa
      961 tctagagctt ctgattcttc aatcaaagga gagatgacat aggcttggga acctttttga
     1021 atttccccct ctaaccaagt caagacctga ggtagttgct catgtttgat ccagcgcgtc
     1081 acaatagact tccgacctgc tggcatctgg tctataatgg aaacatccat atctccaaag
     1141 gctgtgatgg caagcgtccg tggaatggga gtcgccgtca tcatgaggac atctggattg
     1201 tcaccttttt cccgtaaaat acgcctttgc cctacaccaa aacggtgctg ctcatcgata
     1261 ataatcaaac caagacgagc atactccacc ccatcttgta tcagagcgtg agttcctata
     1321 atcaaatcag cctcaccctt ggcaatggtc tgcaagactt ctctcttttc tgcagctttc
     1381 aaggaacctg tcaagagagc caatttcaaa ttgggaaaaa ggttctgtaa actctcaaag
     1441 tgttgctctg cgaggatttc tgttggtacc attagggcag cctgataacc tgctgtcact
     1501 gccgcaaaca tggccaagcc agcgactacc gtttttccac tccccacatc cccttgtagg
     1561 agacgattca tgtggtggtc ggacttcata tcagttaaaa tttcctgcaa actcttttcc
     1621 tgagcttggg tcagggcaaa aggaagactt actttaactg ctgtcacttt ttcctgagac
     1681 caattcagaa ccagaccact tccctgaact ctattttcag acttgagcgt ctgcaactgc
     1741 atttggaaat aaaagagttc ctcaaacttg atacggcgaa gagcctgctt gtattctgcc
     1801 aaatcctttg gaaaatgcat agctcggact gcctgacaac gggacatgag tttgtatttg
     1861 tctagtaaag actggggcag attttcttct atcaagaggt ccagtccctg atcaaaagcc
     1921 gtcttgatga ccttaaccag actggcctga ctgattccct gagccagacg atagacaggc
     1981 tggaggtcat cttctacctg agccagaacc ttcatcccag tcagactagc cttagcgcgg
     2041 tcccattttc caaagacagc aagggttgct cccaactcta ttttatcagc cagatagggc
     2101 tggttaaaga aattcaccgc aaaaacgacc tctccctgct tgagactaaa acgcaggcga
     2161 ttgcgcttga aaccataata ctggacacta gcaggagtca ctacctgacc agaaagaact
     2221 gccttctcac cgtcttccag ctccagcacc tgcttggttt tgaagtcttc ataacggaaa
     2281 ggaaagtaga gcaagagatc ttgcaagttt tcaattccta gtttggcgta tttttctgct
     2341 gactttggtc ccacaccagg caagacatgc aagggttgat gtagattcat gctccactcc
     2401 tttcttttct aataatattc tctcggaata cggtcgctga ggaggcaaac cacctcatag
     2461 ttaatggtta cgcggtaggt cgctacctga gttgcagtga tttccttatc cccattggag
     2521 ccaatcaagg ttaccttggt tccgagtggg taaggcttag gcaatcgaat agtgatttgg
     2581 tccatcgaaa ccctgccgac aattgggcaa gcttggccat ctaccaagac agagaaattt
     2641 tgcatgtctc ttgtccatcc atctgcatac ccgattggca cggtcgcgat gacttgctcg
     2701 ctatccgctt gataagttgc tccatagccc atgcaagctc cagctggaac tgtcttgaca
     2761 tgaaccagag cagactccaa ggtcaaggcc ggtatcaaat cataaggcaa atccaagacc
     2821 gctccacttg gattgaggcc atacatggca tctcccatac gaaccgcatt gaaaatagtc
     2881 tctacatgcc aaagagtcgt tgcagaattg ctagcatgaa ccagctctgg aacttccttc
     2941 atactagcta aaatagtttt aaaccgttct aactgggcat taaaatagtc atctgattcc
     3001 tcatcagcag tagcaaagtg ggtaaagatt ccttcaacac gaacaccgtg ttgttggagc
     3061 aaatcttgag cctgctcaac ctcactggcc tctctaaaac caatccgtcc catccctgaa
     3121 tcaatcttga ggtggactgt caatccagtt aggtccactt ccttatctaa gagtgcttga
     3181 atccactcca gtccagccac tgtcaaggtg aagtcatatt ctttagctag agcaacagct
     3241 tcgatttcag aaactcctaa aatgaggatt ggcttgctga gtccagcttg tctgagttca
     3301 atggcttcat cgatattgga aacgcaaaag ccatcaacat catcttgaat tgccttggca
     3361 acggcaacag ctccatgacc ataagcattg gccttaacca cagccaactt gagcgttcct
     3421 tgagggatat gagcccccat ttgctgaata ttttgtcgaa tagctcccag atgaatcaga
     3481 gccttggttg gtctatgtgg actagctttc atgattttcc tccaaaatga cactggctgt
     3541 cacaaactga tcggtgtggc tgatagacag ccaaatcttt cctgaaaatg gtgcctgact
     3601 aaaataaggc gccccacgtt cattgttcaa gacttccaaa tcctgaaaac cgagcttgct
     3661 aatgcccgtt cccatagcct tggaaaaggc ctccttagcc gaccagcgac cagctaaata
     3721 ttctatttgc ctgcgtcctt tgagactggt gaagcgctcc atttcctgag cggtcagtac
     3781 acgcttagca aatccttcat gtcgtgtaac tgcgctttct atcgaagcca attcttcgat
     3841 gtcaattccg tgtccaacta tcattctcat caaaaggagt tgagattccc caactccatc
     3901 cttttcactt attttgtcaa ggtaacataa atctcttcta ccaaggcctc tgtattttcc
     3961 caacctaggc aagggtcagt aatagagcaa ccaaagatct ctggttggtt ttgacgacca
     4021 tctgctaggt aagattcaat cataaatcct cgaaccgtct ttttaatttt ctcattccaa
     4081 tcacgattct gcaaggtctg gcgaacaatt cgaatctgct ccatatattg cttgcctgag
     4141 ttatcatggt tggtgtcaat gaggataaaa ggattttcaa gtcccatggt ttcatagcgt
     4201 tcaatggctt ggagtaggtt ttcatagtag taattcggca tataattgcc atactcgttg
     4261 actgctccac ggaggataac atgggccaaa ggattacctg atgtctcaac ttcctgccca
     4321 tgataaagga aggtttgctt gttttgagca gcatagatgg cgttaaacat aacacccaaa
     4381 tttcctgagg ttggattttt catccctact ggtgcatcaa tcccagaagc cacaaagcgg
     4441 tgctcttggt cttccacaga acgagctcca acggcatggt agctgaccaa gtcatccacc
     4501 aagatcagat ttgacggata aagcatctca tctgccgttg tcaaaccagt ctctgtaatc
     4561 acgcggtagt gcaactggcg cacagcctgc aagccattaa tcaggcttgg agccttagaa
     4621 gtatctggct ggtgaactaa tcctttatag ccgtctccat tggtacgagg cttagcagta
     4681 tacacgcgca tgaccatgaa aatcttatcc gctaccttct tttgcaaggc ggataaacgg
     4741 cgagcatatt ccaagaccgc ctcttcatta tcagaagagc aaggaccaat caccaagaga
     4801 atacggtcat cttcccctga aataatatct gccaattccc tgtcacggct ttccttgagt
     4861 ctcaaggctt ccgcagacaa ttgggtttca gccttgatga cttccatatc gatttcttga
     4921 cctttttcaa taaatgccat cttattctcc taacgttttg taaatttctc tgacaagagc
     4981 ttctgtgtta tcccaaccca ggcaagggtc tgtgatagac ttaccaaata cttctggctc
     5041 attttgtcga ccatcttcca gataagactc aatcataaaa ccacgaacga actgcttaat
     5101 tttttcattc caagcacggt taatcaaggt ctggcggaca attcggatct gttcaatata
     5161 ctgcttacca gaattgtcat gattggtatc aatgatgata aaaggatttt ccaagcccat
     5221 tttctcatac tgggcaatgg tatcaattaa attgtcataa tagtagttgg gaatattttt
     5281 tccatactca ttaagagcac cacgaagaat agcgtgtgaa agcgggttcc cagttgtttc
     5341 tacttctttt cctaagaaaa ggaaactttg tttgttttga gcagcataaa tcccattaaa
     5401 catgacattg agatttccag aggttggatt tttaaaacca gtagaaaatc ctgccccact
     5461 tgccacaaag cggtgttgct ggtcttcaac tgaacgggca ccaactgcca tgtaagaaat
     5521 caaatcatct acaagcggaa ggttttcagg ataaagcatt tcatcagcag ttgtcatccc
     5581 tgtttctgtg atgacacgat agtgaagatg gcgaacggct ttgattccat tgataagact
     5641 aggcgcttct gtcgcgttag gctggtgaat caagccctta tagccatctc cgttggtacg
     5701 gggtttggca gtataaacac gcataaccat aaagatacga tctgccactt cttcttgtag
     5761 gactgccaaa cgcttagcgt attcaaggac agcttcttcg ttgtcagatg agcatggccc
     5821 gattaccaag agaattcgct ggtcttctcc acgtataatg gcttctagct cttgatctcg
     5881 ctgtgatttc ctctccaaag cctgaccttc taattttgac aaggcacgaa cttcttctat
     5941 atttatttta gagctttttg ctgtaaatac cataactcat ctccttataa agcaaacgga
     6001 tacccagtaa aaatttactt ttcacaagat atccgcctct aaactatctc attttattgt
     6061 cttttaccgt gacagttttt aaatttctta ccagaaccac atgggcaaag ttcattgcgt
     6121 ccaatctggc tcaaatccaa atcttctggc atacttgctt ggtgagcagc gatattgcga
     6181 gtcgctgttg tactgatatg acgttctgcc tgtggtcttt cttgttcatg aatttgtgct
     6241 ttcatcatca agcgtgtcac atcaaactca atcgaaccaa tcatatcatt aaacatacgg
     6301 aaaccttctg cctgatactc aacaacaggg ttgttctgag catagccacg aagtccaacc
     6361 gcgttacgca attgatcaag ggcatcgata tgatctgtcc acttgttatc caccactcgt
     6421 agaatcaaaa ctttttggaa ttctttaact gcttcttcat cgcgtagttt tgaaacctga
     6481 ctatcgtaaa ccttcaaggc acgttggaaa agctcttcct tgatggcctt atcagacaag
     6541 cctgacaagt cttccatcgt aatagaatct tcaggaagca agttgtactt agcaaagttc
     6601 aaaattgcct ctagtttttc atcttgtttg gcacgcgcat gaccatcaac gacacgttca
     6661 atcgtgcgct taatcataga ctgaatttca ggtgccaagt cacgatctgc agtgatgata
     6721 tcgtaacgtt gagcatagat aatctcacgt tgttcacgca tgacatcatc gtattgaagg
     6781 acttgtttac gggtatcgta gttatttcct tcgacacgtt tctgagctgc ttcaacctga
     6841 cgcgtcaaca tgcgagactc aatcgcctct tcagacatgt tcaagcgttc aaagattccc
     6901 ttcaagcgtt cagaaccaaa acgtttcatc aaatcatctt caagagatag gtagaattgt
     6961 gactcacctg gatctccttg acgacctgaa cgtccacgaa gctggttatc gatacgacga
     7021 ctttcatgac gttctgtacc aataacacaa agtcctccaa gttcacgaac accttcacca
     7081 agcttgatgt cggtaccacg acccgccatg ttggttgcga tggtaacggc accacgttga
     7141 ccagcattca tgatgatttg ggcttctcta tagtggtttt tggcattcaa gacttcgtga
     7201 ggaacaccag ctgcgaccaa tttcttagaa atgtagtcac tagtttcaac cgctactgta
     7261 ccaaccaaga caggttgacc cttttggtaa cgagccttaa cgtcttcgac aaccgcttta
     7321 aacttagatt cgatacttgc ataaagaagg tctgagtggt caatacgttg aacaggacgg
     7381 tttgttggga ttggaataac acgaatgttg tagatttcac gaaattcttc ttcctcagtc
     7441 ttacctgtac ccgtcatacc agacaatttt ttgtacatac ggaaaaggtt ttggtacgtg
     7501 attgaggcag atgtcttggt ttcatcctgg attggcacac cttctttggc ttcaatagct
     7561 tggtgcaatc catcagaata acgacgacct tccatggtac gacctgtaaa ttggtcgaca
     7621 atcaagattt cttgctcttc gctcaccaca tagtcaatat cgagaagcat gatgtagttg
     7681 gcacgaaggg cgttatcgat aaagtgggtc aaagccacgt tttcgatgtc atagaggttt
     7741 tcaagtttga agtagctttc agccctgtca atccctgaat cagacaaacc aatagtctta
     7801 gactgcacat cgatgatgta gtcatctttg ttcaaagatt ttacatagtg gtctgccatg
     7861 tgatacaact gactggtttc aaccgcattg gcacctgata caatcaaagg tgtacgagcc
     7921 tcgtcaatca agatagagtc aacctcatcg accaaggcat agttaagcgg acgttgtacc
     7981 atgttttcag cgcgaacgac catgttgtca cgaaggtagt caaatccgat ttctgagtta
     8041 gttgagtaag taatatcaca ctcataggct tcttttttct ccattggaga tttggtagcc
     8101 aagttaatcc ctactgacaa accaagccaa gagtacaatt cacccatctc agtcgcgtca
     8161 cgttctgaca ggtattcatt aaccgtaact acgtgaaccc ctttacctga aagggcattg
     8221 aggtataccg gcatggtcgc agtcaaggtt ttcccttccc ctgtacgcat ctctggcacg
     8281 tcaccatggt gaagaacaat cccccccatg acctgaacct tataagggaa gagacctagg
     8341 acacgtttgg caccttcacg gacaaccgca aatgcttcgt aaagcaatga atccagtgat
     8401 tctccatttt gataacgttc cttaaattca actgtttttg cttttagttg gtcatcagtc
     8461 aaagcagcca tttggtcttc gtatttgaaa accttgtcag ccatcttttc cagacgacgg
     8521 atttctcctt tatcattttc gataattgtt tttaaaatat tagccatgtt tttccttact
     8581 ttaaattccg aatattttag aatgttcctt taattttagc acaattactg attattttca
     8641 aggaagaatc cccattttga aaaggaaaaa gaggttagtt ttcaagctaa cctctccgac
     8701 ttttatgact gtttaattgc caaaaattgg caaaatggca aatgtgataa ataagttaat
     8761 ccaaagagaa agacagcaaa tcagtccaaa aacaaagaga ctgactttct tggacagcaa
     8821 ggccatcaaa atggacagaa ttccaaaaac tagcaagact ttctgtatga tgaagatgtc
     8881 aatccttacc cccagaatcg ctctatccca agggataaag aagaaagaca gccagatcag
     8941 cagaactaaa ccaatataaa ccttggaata gcgtgtaata aatgatttta gatgtgccat
     9001 aagccatctc ctaggattgt atatccttta catttgtata tgtccatttt cgacaactaa
     9061 tacatctgta caaaggtctg aaatatcttc cgatatgtgc gacgtcagga taaccagacc
     9121 attttctttt ttcaggtaag ataaaatgac ctgtttggtt aacctcacac tcttctcatc
     9181 caaagcattc ataggttcat cgagaaagag tatagaagga gaggaaataa aggcttgaat
     9241 caaggccatt ttttgctttg ttcccaagga taaatgacgg tattcaacgt cttcaaattc
     9301 ctgtaaatca tagtattgaa tccaataggc aattcttttt tccgtaactt tagatgaaaa
     9361 ataccttaac agttccaaat tttctctcag ggataaatga gataaaaact cgactttttc
     9421 aattaaaatt cctgcatcct gaatataatg attttttatc ccgtaaactt tcccgtcttg
     9481 aagaactttt cctttgtcaa acttaatata accagcaagt atctttaaaa gaaccgtctt
     9541 gccagatcca ttatcacctt taagtccata aatttttcct gattgaaaca ccaaattcaa
     9601 ctgatttaaa ataggtcttt tcccataaat tttctgtcca tttataattt caagtctcat
     9661 cgtttatact ttcttaaatt tctcttccaa gcgactaaca atcaacataa ctaataagct
     9721 taagagtagt aaatacactg ttacaatatg atccatcatc cattcttgtc ccacaaatgc
     9781 taaaaaatag agaaaaaagg aaaaaccagc gcccgcacta atcagtgcta tcagataaga
     9841 cagtagtaag tacaaaatca ttaactgagc aagtaaaaag aaaagtgcga ctgttttgaa
     9901 atgaattcct agcggaagta aaccaagaaa ccagacaaac aaatcttgag taaaaaattt
     9961 agaaatgaca accaatcgtt tccttgtata atcgaaaaag gagcttgatt gatagcgaat
    10021 caagtgatgg taatggtctg ccaaatcact ttcaagtaaa atgacagaga gccatcctac
    10081 catgaaaagc gaaaggttta acaagagttt ggggatttct aaattcgctt cagaatagtc
    10141 attataggaa aaaattgaaa tcaattctct cttgaaaaag ataacagcat cctgcttttg
    10201 aaccgctgtt gacaatagat ggtttatcaa aatcatcatc agaactatac gaagtagtaa
    10261 aacaatcttt ctctttagcc aaatattgtt aaaattctta aacattatct tcatattttt
    10321 caagcaccat ataagggaca acataaagga tataaggagt aaagagtttc atcaaactta
    10381 catccccata gctagcttgc atcaaactcg tcataggaac catatagtaa ggcaaggcac
    10441 tatagagcag catagaacca agccaaagaa acatcaaata ggtgattgcc gaacgagtca
    10501 cattccccac ataatcaact atttgtaaaa aatagattgc attgatgaaa ataccgatta
    10561 gtaggacaca agtaaagaac aaatagcttt ttatctctcc atctaggagt ctttgtaaac
    10621 cacttccatc agaaaataga gaattccatc caagaggaga aaaagtccca aagaaatagg
    10681 ttacaattgc aattatcagc acagtcacta aatatatcaa acaagggata cttgcaactt
    10741 gcaaagtcaa ctttcttttt aacccttgat aactcacttc tcttccaata ctcaatcgta
    10801 aaactttatt tttcagctca atatattgaa aacctagcaa aacaatgatg attggaataa
    10861 taaatgattg ataaaaacct agattgaact ggagaatacg aaaatctaat gctgatttta
    10921 atagacttgt tcggtaactg taaaaagtgt tatactattt ttatggaaac agtatacgac
    10981 aaagcacaaa aacttaactc aaaaaacttc aaactattga ttggtgtcaa aaaggaaacc
    11041 tttcaactca tgctagaaca cctgaattca gcctatcaga ttcagcaccg aaaaggtgga
    11101 cgtccacgta gtctgcccat ggaagaccag ctcattatga ccctccgtta cttgcgatat
    11161 tatcccactc agcgtctgct ggcctttgat tttggcgtcg gtgtagctac ggtaaatgcc
    11221 atcatcactt gggtggagga tacacttcgt gcgtcaggta gctttgattt ggaccattta
    11281 gaagccccga gtgctgctgt ggctattgac gtgaccgaaa gtccgattca gcgtccaaaa
    11341 aaaaccaaag caaaaattat tctggtaaaa agaaacgaca caccttaaaa actcaaatta
    11401 tgctggattt gacgacacat aaagtctgtc aaatggcctt ttctgacgga catacgcatg
    11461 attttactct cttcaaagaa agtattggac aaagtttgcc tgaaacgacg cttgcctttg
    11521 ttgacctagg ttatttaggc atcttgaaat ttcatgagaa tactttcatt cctgctaaaa
    11581 attccaaaaa tcgccgcctg agtgaggatg ataagcagtt aaataaagag atgtcagcga
    11641 tacgaattga aattgaacat tttaacgcta aattcaagac cttccaaatc atgtcagtcc
    11701 cttatcgtaa ccgcagaaaa cgtttcgagt tacgggcgga attaatttgt gccatcatca
    11761 attatgaagt gaactagatt ctgaacaagt ctaataagtc atttttaatt gcttgtccat
    11821 cgaatttagc tagatcgggc gcatgaatat caaaaaagaa ataagtcatc aaaacatcac
    11881 gataaaccaa agcaagaaaa agcaaagtaa gcaagacata aacaaattta tgccttgcca
    11941 aagttaagag aggaatcttc ataagccacc tcctacaaat agaaagtaat ataatactca
    12001 atgaaaatca aagagcaaac taggaaacta gccgcaagtt gctcaaagca ctgctttgag
    12061 gttgtagata agactgacga agtcagttac atatataatc caaggcgacg ttgacgtggt
    12121 ttgaagagat tttcgaagag tataaatcaa tgccttccgc ctttagactt ccctagttcc
    12181 cgtctcaagt caagcatttt tttgaaacag atatagtcta accagtcact agcaaaagcg
    12241 ataagtagta ataatagcca aataccccat aacttgatat ctgtcaaatt tctcaagacg
    12301 atattgaaaa acagaactga aaaaactgtc caagaaaggc taaaaagagc atagaggggg
    12361 atgtaaatcc agtaaaaata gtaaaaaatt gggaaaaatt tactttctct attatccttg
    12421 tcaatccagc tgaaaaagta aaaacagggt aataagatta tcattttaaa caagtgttcc
    12481 atcacaagcc tcctttttta aattgttttc tttagtttcg ttctattaaa tgttattacc
    12541 aatataaacc ttggaatagc gtgtaataaa tgattttaga ggtaccataa gccacctcct
    12601 acaaatagaa accgatataa atcaatgcct tccaccctta gacttcccta gttcctgtct
    12661 caagcgaaac atttctttga aacaggaata agttaaccaa ttcataccaa tagctagcag
    12721 aataaaaaga aaccaaatgc cccataactt gatatctgtc acatttctca agacggtatt
    12781 gaaaaacaga actgaaacaa ctgtccaagc aaggctaaaa agagaataga aggggatgta
    12841 aatccagtaa aaataataaa aaattggaaa aaacttacta tttctgttgg ccttttcaat
    12901 ccagttatca aaataaaagt acggtgctaa aagtaagaat ttaaacaaat gttccatcac
    12961 cgacatcccc ctccttcttt tgatagcgtt ttctattatt ttattatatc aaaagaatct
    13021 ggaaatgtca ttccagattc tactttttta tttacgcttt cttgcgatga gatgaatcgg
    13081 tgttccctca aagacaaagg ccttgcggat ttgattttcc aagaaacgta ggtaagaaaa
    13141 gtgcatgagt tcttcttcgt tgacaaagat aacaaaggtt ggtggtttgg ttgccacttg
    13201 ggtcgcatag aaaatcttga gacgttttcc tttgtctgtc ggtgttgggt tgatggcaat
    13261 ggcatccatg atgacatcgt tcaagacagc tgatggaata cgtgtatttt gactttcgct
    13321 gatttgctta atcatcccag gaagtttgtg gagacgttgc ttggttaaag ctgatacaaa
    13381 gataatcggt gcgtaaggca ggtattggaa ctgctcacgg atatcttctt cccagttttt
    13441 catagtgtgg ttatcttttt caagcgtatc ccacttgttg accacgataa tcatcccttt
    13501 accagcttca tgagcaaatc cagcgatacg cttgtcgtat tcacgaatcc cctcttccgc
    13561 attgatgacc attaagacca catctgaacg gtcaatagca cgcatggcac gcatgacaga
    13621 gtacttctca gtattttcat aaaccttacc agacttacgc ataccagccg tatcaatcat
    13681 ggtaaactct tgaccatctg tatctgtaaa gtgggtatca atggcatcac gagttgttcc
    13741 agcaacagga ctggcaatga cacggtcttc tcccaagata gcattaatca agcttgattt
    13801 tccaacgtta ggacgaccaa tcaagctaaa cttaatgaca tctggatttt cttcttcata
    13861 ttcatttgga aggttttcca caatcgcatc tagtacatcc cctgtaccga ttccatggac
    13921 agatgagata ggcagtggtt cacccaaacc gagagcatag aaatcataga tatcatttct
    13981 catctcaggg ttgtccacct tgttgactgc gaggataact ggtttgtggg tcttataaag
    14041 cttacgagct acgtattcgt ctgcatcagt aattccttcc ttaccagaca cgacaaaaac
    14101 gataacatct gcttcttcca tggcaatttc tgcctggtgc ttgatttgtt ccatgaaagg
    14161 agcatcgaca tcatcaattc ctcctgtatc aatcatacta aaagagcgat tgagccactc
    14221 acccgttgca tagatacggt cacgtgtcac tccttcgaca tcttctacaa tggagattcg
    14281 ctcaccagcg atccgattaa atagggttga tttcccaaca ttgggacgtc ctacaatggc
    14341 aatagttggt agggccataa tttctcactt tctacaataa cttcttctgt ttaagatttt
    14401 ttctagttga gcttggttct gcttggccaa actgttctgc tagacgctga ctccagcttg
    14461 tggtcgcacg cgctccagca tagtcagcct gaacacggtc ataagcttgg attgcctcag
    14521 ttgactgttc ttggtattct tcctcaaaga caacattctc tagtggcagt ctcggtttca
    14581 tatcatgatg ttgatttggc acacccagtg ccatcccaaa gacagaatag gtgtagtcag
    14641 gtaggttaaa gagctctgcc acttcttcag acttgtatcg aaccaaaccg ataatcacac
    14701 caccatagcc caagctttca gctgccaaca aggcgttttg tccagcaaga gctgcatcga
    14761 ccgaactaat caagagacct tccacacctt ggggttggaa ggtgtcggta tgaagtcggg
    14821 ctcccttttc tgctcggttc aaatctccga caaagagaag gaaaacagca gactggcgaa
    14881 tggcttcttg aggtaccaat tcatacaagg catctttctt ctcttgactt cgtaccacaa
    14941 tcacagagta ggattggaaa ttcttccaag atgatgccat ctgggctgct gtcaaaatct
    15001 catttaagtc tacttgggga atttcttgct ctttaaacct gcgcactgaa gtatgagcct
    15061 tcatcaattt aatggtttct gtcatcgacg gtttactcct tctaaacgag tctcctcagc
    15121 caaataacgg atgcgttcca tgacacgtct ggcttcccag gtttcgtcat ttccattttt
    15181 ccctttagcg aaatgcttct ccaaatcttc aaagttgaag ttagaggtga aaaaggtcgg
    15241 taaattttcc tgcatccgat attggagaat gacctgcagg atttcatcac gcacccaagc
    15301 ggtggattgc tcggcaccaa tatcatctaa aatcaggact tcagaaagct taatctcatc
    15361 cactaaggtc ttaacattgc catcaccgat agcatttttg acatcaatga caaagctagg
    15421 atagtggagg agagtggatg atacaccacg tttttctgat aaatcatgag ctagggcagc
    15481 caccatgaaa cttttaccca caccaaagtc tccatataag taaagacctt tcaaattatt
    15541 ggggtaatcg ttaacaaatt ctatcattct ttttagaaca gttaagcgct gaacatcatc
    15601 acgataaaca tctaaaaaac tgacattttt caggctagat ggaaaattaa ttaagttgag
    15661 acgtttcttg atagccgctt ctttttcagc tgcgattagt tcaggagttt cttcataaga
    15721 aacatccgca tagccatggt tcatgactaa tataggcttg tagcctttgg caatataatc
    15781 tgtatctcct cggaggaatt tatctctctc agtgatgtat tggttaaact tggaaatact
    15841 acggttcaac tcgtcctgat tgagggattc ttgctggaca aaggccgcaa catcaggatc
    15901 cttcatgatt ttctgaacta aatcttgata gtgaaaacga cttggttgac gtttgagtac
    15961 atctccgaca ctttccatct aatcacctcc gttatctaat tttgctaata attctagggt
    16021 ttggcgttcc atctcttcta attcctcagc ggatgacgta tttttatatt caggattact
    16081 ccatttagga atattggttt ttgctggggc agtctgattc tgtttttgtg tttttgtttt
    16141 ctgccctcgc tcacggatgc gcaagactgc cttttctgcc gaatgaatct tttgataggc
    16201 atagtcattg gctaccttca tggcatattt ctcattgata tttgccgaat cgaccttatt
    16261 aaaggtcaat aaaagaatga tattgatgac ttcgtccagc aagcccaagc cagccatctg
    16321 ttgcaagagt tctctttctg tttgggtaat ggttcccttg cgtgtttgct tgatttctgc
    16381 caagaactgc agggcagttt tacttttggc ttctttgata atggttgttt ccttaagact
    16441 aaagtcagag gaaactggtt tttgagcaat tttttcacgc atgcgtttgg ttgaaataac
    16501 ctgagaaaca gctgttgact tggccaattg ataggtttca aaccaagtcc atttcttctc
    16561 ctcggcaata gcaaagaggt ttaagacatc ggactgctca tccgcaaaac gaagtccatc
    16621 tcgagccatc agctggcgaa aatgttccaa gtcaaaatca ttggccactt tcttcttgag
    16681 accaaggtct tcttgactgc ctagttctgc caattctgga aagacttgat tgagtgagac
    16741 aggtatttct tcaccatcag cactttcaac tttcaaatcc tccacagcag agtcgccaat
    16801 ctttttctct aagagtctgc gataaacagg atgctccaag aagtcttgac tagatagagg
    16861 agcatggagg gctagctgat aaacatcccc cttttgatag agggtcaaga gattaaaagc
    16921 agataagatt ttcaatgatt ttatcagtct atccatccca aagttgagat gattgaggat
    16981 acttgaaaaa agatattcct ttctaccatt atcccaaaaa ctgattgtat aaagataaag
    17041 gctcagtgcc tcctgaccga taatcgggag gtagcactgt accagagatg aggtatcttg
    17101 cgacacccga ttattcttta gataagaaaa acggtcaatt ggcttcattt atctttcctt
    17161 tttcttttta gaggactggg tgatttgttg gagcaagctc tctaactcac tgacatcctt
    17221 aaaactacga tagacactag caaaacgtac ataggtaatc tcgtccaatt cagccaactc
    17281 ctccatgaca agtgaaccaa tgtcctcact ttgaatttca ttttcatttc gaccacggag
    17341 tttctgttcg atacgattga ctaccatgtt gatttcatca cttgacacag gacgtttctg
    17401 ggctgagcgg ataatcccat taaagatttt atctctggag aattgttccc gtgtgccatc
    17461 ttttttaaca accactaagg ttctttcttc tactcgttcg taggttgtaa aacggtgttg
    17521 gcactcgtcg cactctcgtc ttctacgaat ggtgttccct tcttctgctt ggcgactatc
    17581 gataacactt gacttggtag ccccacattt tggacaacgc atcctttccc tccttatcgt
    17641 tttcttttca ttataccatt ttttaagcga ttcccaaaac aattcttctt tttgcttgac
    17701 aagttttttg ttttgttgta ttatttaatt aagacaaaag gataagagaa aggagactaa
    17761 gatgtcctgg acatttgaca acaaaaaacc catctattta cagattatgg agaaaatcaa
    17821 gcttcagatt gtttcccata cactggaacc caatcaacaa cttccaaccg tgagggagct
    17881 agctagcgag gctggtgtca atcccaatac catccaaaga gccttatcag accttgaacg
    17941 agaaggattt gtctacagca agcgaacaac tggacgattt gtgactaagg ataaggagct
    18001 aatcgctcag tcacgcaaac aattatcaga agaagaattg gaacacttcg tttcctccat
    18061 gacccatttt ggctatgaaa aagaagaact accaggcgta gtcagtgatt atattaaagg
    18121 agtttaagcc tatgtcatta ctagcatttg aaaatgtatc caaatcatat ggagcaacac
    18181 cagcccttga aaatgtttct cttgacattc cagctggaaa aattgtcggc cttcttgggc
    18241 caaacggctc aggaaaaaca accctgatta aactaattaa tggcctctta caaccagatc
    18301 aaggacgtgt cctcatcaac gacatggacc caagcccagc aaccaaggcc gttgtagctt
    18361 atttgcctga tacgacctat ctcaatgagc aaatgaaggt caaagaagcc ctaacctact
    18421 tcaagacctt ctataaagat ttcaatcttg aacgcgctca tcatctactt gcagacctgg
    18481 gcattgatga aaatagtcgt ctcaagaaac tatcaaaagg aaacaaagaa aaggttcaac
    18541 tgattttggt tatgagccgt gatgctcgtc tctatgtttt ggatgaaccc attggtgggg
    18601 tggatccagc agcccgtgct tatatcctca ataccattat caacaactac tcaccaactt
    18661 ctaccgtttt gatttctacc cacttgattt ctgatatcga gccaatcttg gatgaaattg
    18721 tcttcctaaa agacggaaaa gtcgtccgtc aaggaaatgt agatgatatt cgctacgagt
    18781 caggtgaatc cattgaccaa ctcttccgtc aggaatttaa ggcctaagca aaggagatta
    18841 tttatgtttt ggaatttagt tcactacgaa tttaaaaatg ttaacaagtg gtatttagcc
    18901 ctctacgcag ccgtgctagt cctttctgcc ctcatcggaa tacagacaca aggctttaaa
    18961 aatctacctt accaagaaag tcaggctact atgctacttt ttctagctac agtctttggt
    19021 ggcttgatgc ttacacttgc gatttcaacc attttcttga ttattaaacg cttcaaaggt
    19081 agtgtctacg accgacaagg ctatctgact ttgaccttgc cagtttctga acaccatatc
    19141 atcacagcca aactaatcgg tgcctttatc tggtcattga ttagcaccgc tgtattggct
    19201 ctaagtgctg ttattattct ggctttaaca gctccagaat ggattcctct ttcttatgtg
    19261 attacatttg tagaaacaca tctccctcag atctttctta caggtatatc cttcctacta
    19321 aataccattt caggaatcct ctgcatctac ctggctattt ccattggaca gcttttcaat
    19381 gaataccgta cagcactcgc tgttgcagcc tacattggta tccaaatcgt cattggattt
    19441 attgaacttt tcttcaatct tagttccaat ttctatgtca attcactggt aggactcaat
    19501 gaccatttct atatgggagc aggtatagcc attgttaaag aactcatatt catagctatc
    19561 ttttatctcg gaacctacta catcttgaga aataaggtta atttgcttta aataattttt
    19621 acctagatat gtaacatact catagaacaa aagagaccag gcaaaaagtc tttaaaatta
    19681 gaaaacgcat agtatcaagt gttaaaaatc cttgatatta tgcgtttttt gtgagctgac
    19741 ttatttcctt tcgctatatc gcaaaatgaa ataagaacgg aacgatggga ttttggaatt
    19801 caaatcaatt tataagaatg ttttagaagt aatattatcc tattccagat tcagttcact
    19861 atacaattga gttttcaagc aacctgttta cataatgtgt acataattag gttcgtgatt
    19921 ccaccctttt cacctttaaa aacctcgctt tcgcaaggct cttctattta taagataagg
    19981 cacgtttaaa ggttttccaa atccctaaat catccgtttg aagaacgaga ctagcataca
    20041 tgcgtccgat aaatcctgtt gctaccaccg caaaaatcac tgtaatagca agtgaaatcc
    20101 atgcttccgc tccctccgca tagccattaa tcgttcgaaa cggcataaag aaggtcgaaa
    20161 taaagggaat ataagaacca atcttcaaga ggagattgtc accagctgca cctagagctg
    20221 tcactccaaa aaaaccaccc ataatcaaaa tcatcaaagg cgacaaggct ttccctgagt
    20281 cctcaggacg agaaaccata gatcctagga aggctgccaa gactacgtac atgaaaagac
    20341 tgatcaaaat aaagagcagg gtattcagtg agatagcatc tcccaagtga tccaaaatac
    20401 cagactgagc caagaatggc aaatctttaa agagcaaaac ggcagccgac cacctacaac
    20461 atagatccca atatgcgtta aaatcactag aaacagagcc atcatccgcg catagaaata
    20521 gtgacttgcc cttatgctag aaaagacgac ttccataatt ttggtgcctt tttcactggc
    20581 aacttcctga gctgttacac ccgcataggt aatcagaatc atataaagaa agaatcctaa
    20641 ggcacctgct gcaattgttt gaataaactt tttattttcc ttggcttcat caatcttttc
    20701 tgtgaattga attgtctgcg ctaagcgttt ttcctgctct tgagacaagg aagcagttga
    20761 acgattaagc tgattttgca gttcattgag tgtacctgta acctcaaatt taattccatt
    20821 ttcaagcgat gtttcgccat gataaactgc ctttagaaca ctatcttctt gatcaatggt
    20881 caaataacct tttaattttt cttctttaat tgcttctttg gcacttgctt catctttata
    20941 gtcgaagtta acaccattta cattcttcag tccttctgct acagatggca ctgttgtcac
    21001 tactgccact ttattatttt tagccataga agaaccttgg agatgcccaa ttcctacaga
    21061 gattcctaaa aagaggaacg gcgaaatcac cataaagaag aaactccatg actcgacatg
    21121 tcgaagatag gtttccttga ttacaaccca catatttctc atacttccac tcctgattct
    21181 agtttaaaga tttcatcgat agttggcgct tgttggtcaa atgttgcgat atattgacct
    21241 tgagtcaaga ttgggaagag ttcccttcca gcgctctcat cctccaaaat caatttccaa
    21301 ctgccttgtt tggtcaagct cacctgtttg acatgaggaa gattttccaa ttcttccttg
    21361 cttcgttcac ttgaaacaaa gagacgcgtt ttcccgtatt gattgcggac atcctgaact
    21421 ggtccgtgca agaccacacg gccatctcgg atcatcagaa tatcgtcaca aagttcctca
    21481 acattggtca tgacatggtc agaaaagata atggttgctc cgcgctcttt ttcctgaaaa
    21541 atgacttgtt tgagcaattc tgtattaact gggtccaatc cactaaaagg ctcatccaag
    21601 ataatcaggt ctggttcatg aatcagagta ataatgagct gaatcttctg ctgatttcct
    21661 tttgacagac tcttgatttt atctgtcagc tttcctttca cttccaacct cttcatccat
    21721 tgagggagtt tttctttgac ttctttggca tccatgcctt ttagagtcgc caagtagcga
    21781 acttgttcaa gaactgtcaa tttaggcatg agactgcgtt cttcaggcag ataaccaatc
    21841 cgagcatagg tctcctgacg aatatcctga ccatccagac cgatttctcc ctgatattct
    21901 aggaatttca aaatactatg gaaaatcgtt gtttttccag caccattttt tccgactagt
    21961 cccaaaatac gacctggtcg cgcttgaaag tcaataccaa acaaaacttg cttggagcca
    22021 aaacttttct ctagacttct tacttctagc atctttcacc tccgaaattt cttgcactca
    22081 ttatactcct ttttgatagc ctttacaatg tttttgtcca tttttagaag actattgctg
    22141 tgtaaaatat ggcctggagc acttttatac tcaatgaaaa gcaaagagca aactaggaag
    22201 ctagccgtag gctgctcaaa gtacagcttt gaggttgcag ataaaactga cgaagtcagc
    22261 tcaaaacact gttttgaggt tgtggataga actgacgaag tcagtaacta tatctacggc
    22321 aaggcgaagc tgacgtggtt tgaagagatt ttcgaagagt attagtgata aatccattat
    22381 acagtagcaa acttaattta taccttccgc tcctcaactg tctattttta atcctgaatt
    22441 gttatttgag taactccttt ttcctcataa agttttcttc ctctaaaact tctggaaaaa
    22501 agcgaatagt ttcagacaac atttttataa gaaacaagtt catctgtcat ttcaagaagg
    22561 agtaatcctt tatctactaa tggacggaac ggaattcaac cgcttgtccg atatgttttc
    22621 taaggattat atagcaaaat gaaataaaaa caggacaaat agatcgggac agtcaaatcg
    22681 atttctaaca atgttttaga agtagatgcg tactattcta gtttcaatct attatatcta
    22741 ttatgcacac cactatagga tctaataaaa atcacaacaa gttcattcat agatggttac
    22801 ctcagggaac taagaaaacg actacaaagg aagtcgcatt catcgaaaag tggattaaca
    22861 actatcctaa aaaatgcttg aactacaagt cccccagaga agacttctgg atgactaact
    22921 tgaacttgaa atttagcaat aattaattca ccatctaact atatttagta attatttcag
    22981 aactgattaa tattaaaatt aactaacaat tcaaaggatt catactagcc ataaattacg
    23041 tccatcagag agagactctt actactttta gattttagtc tttctagctt cagaatacat
    23101 ctaaacttta gggaaaatga ctattcgaaa gcgcgaatgc ctcaaaatta tctcagataa
    23161 gctattcgaa acttagaatg cttttaaatt catggaattg cgattattcg aaacctagaa
    23221 tgcatataac ctttagttga cagacctatt ctaagtctcg aagggctatt tactttctat
    23281 tccttatcaa aaaagactca ttcccccttt ctcctccaaa atatggtata gtagaaatat
    23341 actatctatg aggagtttac atgtcacagg ataaacaaat gaaagctgtt tctccccttc
    23401 tgcagcgagt tatcaatatc tcatcgattg tcggtggggt tgggagtttg attttctgta
    23461 tttgggctta tcaggctggg attttacaat ccaaggaaac cctctctgcc tttatccagc
    23521 aggcaggcat ctggggtcca cctctcttta tctttttaca gattttacag actgtcgtcc
    23581 ctatcattcc aggggccttg acctcggtgg ctggggtctt tatctacggg cacatcatcg
    23641 ggactatcta caactatatc ggcatcgtga ttggctgtgc cattatcttt tatctagtgc
    23701 gcctatacgg agctgccttt gtccagtctg tcgtcagcaa gcgcacctac gacaagtaca
    23761 tcggctggct agataagggc aatcgttttg accgcttctt tatttttatg atgatttggc
    23821 ccattagccc agctgacttt ctctgtatgc tggctgccct gaccaagatg agtttcaagc
    23881 gctacatgac catcatcatt ctgaccaaac cctttaccct cgtggtttat acctacggtt
    23941 tgacctatat tattgacttt ttctggcaaa tgctttgaca cgtaaaaaat ccgtttggtt
    24001 tcccaagtgg attttgtgct ttattttgaa acttcttttg caagaacaaa gttcccaagt
    24061 gtggcagaac catttcctgc gactgctggc gtcacgatat agtcacgcac atctggtact
    24121 ggtaggtaac cattaagaag agatgtaaat ttctcacgga cacggtccag catatgttgt
    24181 tgagccatga cccctccacc aaagacaatc acgtctgggc ggaaagtcac tgtcgcatta
    24241 accgcagctt gagcgatata gtaggcttga acatcccaaa cagggttgtt gagttcaata
    24301 gtttccccac gtacacctgt acgagcttcc aaacttggac cagctgcata accttctaga
    24361 catcccttat ggaaaggaca aacaccctta aactcttttt caatatccat tgggtgtcta
    24421 gcaacataat aatgacccat ttcagggtga cccacaccac cgataaactc accacgttgg
    24481 atgacgcctg caccgatacc tgtaccgatt gtgtagtaaa ccaagttttc gatacgacca
    24541 ccagcattgt tacgggcaac catttcaccg taagcagagc tgtttacgtc tgttgtgaag
    24601 tacattggca cgtttagggc gcgacgaagg gcaccaagca agtctacatt tgcccagttt
    24661 ggttttggag tcgtcgtgat aaagccataa gtttttgagt ttttgtcaat atcaatcggc
    24721 ccaaatgaac caactgcaag accagcaagg ttatcgaatt ttgagaagaa ctcaatggtt
    24781 ttatcgattg tttcgattgg agttgttgtt ggaaattgtg ttttttctac aacgttaaag
    24841 ttttcatcac cgacagcaca gacaaacttt gtaccgcccg cttccaagct tccatataat
    24901 tttgtcatga taaacctctt gtttttattt tctttattat agcatacttc gaaagtctaa
    24961 atgtctctat tttttagatt ttcctctgta aatcttacta tctaataaaa acgaacaaac
    25021 atgtcatttg ttcgttttca cattagagag gattgattag attttcactt cgatcacagc
    25081 atcccccttc gaaactgaac ctgttgcgac tggagctact gaagcgtagt cagctgtatt
    25141 tgtaacgata accattgttg tatcatcaag tccagctgca gcgattttgt ttgagtcaaa
    25201 tgttccaaga acatcgccag ctttcacctt attaccttga gcaacttttg cttcaaaacc
    25261 gtcaccgttc atagatacag tgtcaatacc aacgtgaatc aaaacttcag caccatttct
    25321 tgttttcaaa ccaaaagcgt gccctgttgg aaaggcaatt gaaacttcag catcagctag
    25381 tgcatagacc acgccttgac ttggtttcac agcgatacct tgtcccatag ctccacttga
    25441 gaagactggg tcattgacat cagcaagagc gacaacatca ccgacgatag gagttacaag
    25501 tgtttcattt tgaagagctg ctggcgcaac ttcttctttt tcttcagcca cttcagcttg
    25561 ttttgcagct gcagttgcgt ctacttcatc ttcgtaacca aacatgtaag taagagcaaa
    25621 accaagggca aatgatacag ctaccataag aaggtattgt ggaagttgtc cgttaccaac
    25681 ataaagcatt gtaccaggga tgatggtgat accattacca gtaccagcaa gtccaaggat
    25741 agaagccaat ccaccaccga ttgcaccagc aatcaatgaa aggaagaatg gtttacggaa
    25801 gcgcaagttc accccgaaga tagcaggctc tgtaatacct aggaaggcag aaagagcagc
    25861 cgggaaagca agtgttttca gttttggatt ttttgtttta acaccaaccg caacagtagc
    25921 agcaccttga gctgtcatag cagctgtgat gatagcgttg aatgggttag catggtcagc
    25981 agcaagtaat tgcacttcaa gcaagttgaa gatgtggtgc acacctgaca cgacgatcaa
    26041 ttggtgaacc ccaccaatca agaaaccacc aagaccaaat ggcatgctaa gaatcgcttt
    26101 tgtagcaata aggatgtagt tttcaacaac gtggaaaact ggtccaataa caaagagtcc
    26161 aaggatagac atgaccaaaa gtgtcacgaa tggtgttacc aagaggtcaa tgacatctgg
    26221 aacaaccttg cggacagctt tttcaaattt agctccgaca accccgatga tgaaggctgg
    26281 aagaacggaa ccttgcaaac caacaacagg gatgaaacca aagaagttca tcgctgttac
    26341 ttcaccacct tgagcaactg cccaagcgtt tggaagtgag ccagagacaa gcatcatacc
    26401 aagaacgata ccaacggcag gatttccacc aaatacacgg aaggttgacc acacaaccaa
    26461 acctggcaag atgatgaagg ctgtatctgt caagatttgt gtgtaagttg caaagtcacc
    26521 tggaagtggc atttcaagag cgttgaaaag accacgcaca cccatgaaga gacctgtcgc
    26581 tacgataact gggatgattg gaacgaaaac atcaccgaaa gtacggatag cacgttggaa
    26641 ccagttccct tgtttagcaa cttctgcttt catgtcatcc ttagatgatg ttggtaagcc
    26701 aagtacaaca acttcatcgt acattttgtt aactgtacct gtaccaaaga taatttggta
    26761 ttgccctgag ttaaagaaag caccttgaac tttttccaag ttctcaatca cttctttatt
    26821 gattttctct tcatctttga ccatgacacg tagacgagtc gcacagtggg caacactatt
    26881 gacattttca cgtccgccca aggcatcgat gacttttttt gcaatttcct gattgttcat
    26941 ttgcaaaaat ctccttatat aacattttgt tcttgtttga aagcgatttt attcgccctt
    27001 acgactatta ttttatcatg ttttaaaaat atgtcaagcg ttttgcagaa aaaatattct
    27061 atccacttag tgggcaagtt ttagattgat tgtttttgac tgtttcatca ggaattcctt
    27121 gaaaaataag tttgaaacct tggttaaaaa gtattttatt tcattatctt tcacattttc
//
LOCUS       DAVKGU010000019        26653 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKGU010000019 DAVKGU010000000
VERSION     DAVKGU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.019"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            115..663
                     /locus_tag="VW077_000787"
     CDS             115..663
                     /locus_tag="VW077_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9454841.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            773..1675
                     /locus_tag="VW077_000788"
     CDS             773..1675
                     /locus_tag="VW077_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9454842.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKRSS"
     gene            complement(1696..2970)
                     /gene="serS"
                     /locus_tag="VW077_000789"
     CDS             complement(1696..2970)
                     /gene="serS"
                     /locus_tag="VW077_000789"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9454843.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3184..3555)
                     /locus_tag="VW077_000790"
     CDS             complement(3184..3555)
                     /locus_tag="VW077_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9454844.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIARKKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3558..4922)
                     /locus_tag="VW077_000791"
     CDS             complement(3558..4922)
                     /locus_tag="VW077_000791"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9454845.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5247..6032
                     /locus_tag="VW077_000792"
     CDS             5247..6032
                     /locus_tag="VW077_000792"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9454846.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6126..6560
                     /locus_tag="VW077_000793"
     CDS             6126..6560
                     /locus_tag="VW077_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9454847.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6560..7534
                     /locus_tag="VW077_000794"
     CDS             6560..7534
                     /locus_tag="VW077_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9454848.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7594..7818
                     /locus_tag="VW077_000795"
     CDS             7594..7818
                     /locus_tag="VW077_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9454849.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            7937..8911
                     /gene="fabK"
                     /locus_tag="VW077_000796"
     CDS             7937..8911
                     /gene="fabK"
                     /locus_tag="VW077_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9454850.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            8904..9824
                     /gene="fabD"
                     /locus_tag="VW077_000797"
     CDS             8904..9824
                     /gene="fabD"
                     /locus_tag="VW077_000797"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9454851.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9858..10589
                     /gene="fabG"
                     /locus_tag="VW077_000798"
     CDS             9858..10589
                     /gene="fabG"
                     /locus_tag="VW077_000798"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9454852.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            10611..11846
                     /gene="fabF"
                     /locus_tag="VW077_000799"
     CDS             10611..11846
                     /gene="fabF"
                     /locus_tag="VW077_000799"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9454853.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11849..12334
                     /locus_tag="VW077_000800"
     CDS             11849..12334
                     /locus_tag="VW077_000800"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9454854.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12331..12753
                     /gene="fabZ"
                     /locus_tag="VW077_000801"
     CDS             12331..12753
                     /gene="fabZ"
                     /locus_tag="VW077_000801"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9454855.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            12765..14132
                     /gene="accC"
                     /locus_tag="VW077_000802"
     CDS             12765..14132
                     /gene="accC"
                     /locus_tag="VW077_000802"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9454856.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14169..15035
                     /locus_tag="VW077_000803"
     CDS             14169..15035
                     /locus_tag="VW077_000803"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9454857.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15032..15799
                     /locus_tag="VW077_000804"
     CDS             15032..15799
                     /locus_tag="VW077_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9454858.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            15958..16143
                     /gene="briC"
                     /locus_tag="VW077_000805"
     CDS             15958..16143
                     /gene="briC"
                     /locus_tag="VW077_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9454859.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            16506..16883
                     /locus_tag="VW077_000806"
     CDS             16506..16883
                     /locus_tag="VW077_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9454860.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYLMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(16932..17354)
                     /gene="nusB"
                     /locus_tag="VW077_000807"
     CDS             complement(16932..17354)
                     /gene="nusB"
                     /locus_tag="VW077_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9454861.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(17347..17736)
                     /locus_tag="VW077_000808"
     CDS             complement(17347..17736)
                     /locus_tag="VW077_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9454862.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(17758..18318)
                     /gene="efp"
                     /locus_tag="VW077_000809"
     CDS             complement(17758..18318)
                     /gene="efp"
                     /locus_tag="VW077_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9454863.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(18447..19889)
                     /gene="gatB"
                     /locus_tag="VW077_000810"
     CDS             complement(18447..19889)
                     /gene="gatB"
                     /locus_tag="VW077_000810"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9454864.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(19889..21355)
                     /gene="gatA"
                     /locus_tag="VW077_000811"
     CDS             complement(19889..21355)
                     /gene="gatA"
                     /locus_tag="VW077_000811"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9454865.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDTKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(21355..21657)
                     /gene="gatC"
                     /locus_tag="VW077_000812"
     CDS             complement(21355..21657)
                     /gene="gatC"
                     /locus_tag="VW077_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9454866.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            21870..23414
                     /locus_tag="VW077_000813"
     CDS             21870..23414
                     /locus_tag="VW077_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9454867.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVSGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            23669..23867
                     /locus_tag="VW077_000814"
                     /pseudo
     CDS             23669..23867
                     /locus_tag="VW077_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
     gene            23978..24154
                     /locus_tag="VW077_000815"
     CDS             23978..24154
                     /locus_tag="VW077_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9454868.1"
                     /translation="MDLDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            24270..24458
                     /locus_tag="VW077_000816"
     CDS             24270..24458
                     /locus_tag="VW077_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9454869.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            24615..24980
                     /locus_tag="VW077_000817"
     CDS             24615..24980
                     /locus_tag="VW077_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9454870.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            24983..26650
                     /locus_tag="VW077_000818"
     CDS             24983..26650
                     /locus_tag="VW077_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9454871.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALRD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         7649 a         5062 c         6170 g         7772 t
ORIGIN      
        1 taacaacgaa aatcttatga gagaataaag ctttatctta gataaactta ggaatcagga
       61 ttttataaga ttttccaata atattagtgt caataataag aaaagaggta tcttatgaca
      121 acatttacta tccatacagt agagtcagca ccagcagaag tgaaagaaat tcttgaaaca
      181 gtagaaaaag acaacaatgg ctatattccc aacctaatcg gtctcttggc caatgccccg
      241 actgttttag aagcctacca aattgtctca tctatccacc gtcgcaacag cctgacaccc
      301 gttgagcgtg aagtggtgca aatcacggca gccgtgacca atggttgtgc cttctgtgtc
      361 gcaggtcaca cagccttttc cataaaacaa atccagatga atgatgactt gattcaagct
      421 cttcgcaatc gtactccaat tgaaacagat cctaaattgg ataccctagc taagtttacc
      481 ttggcagtta tcaataccaa gggtcgtgta ggagatgaag ccttgtctga gtttttagaa
      541 gctggctaca ctcaacaaaa tgccttggat gtggtttttg gtgtcagcct agcaatcctc
      601 tgtaactatg ccaacaactt agctaataca ccaattaatc cagaattgca accttatgcc
      661 taattcatat ctgagtaaaa tgaagtctga aataagcgga cttcgttttt ttaaatcctc
      721 atttaagcgc ttttatgtta tactgaaact aacatgatta ttggaggtta ggatgaaaaa
      781 actcccctta gtattttcag gttgtttgct aggtttggca ggagctggaa atcttatttt
      841 agatacgttg ccggttctat cccatctttt tagtctgact ggtttggttt tatggattta
      901 ctttctaatt ctgcatctct ttaattggaa agaaaccaag caagaattga ccaagccccc
      961 tcttttgtca ggaatggcaa cctttcctat ggctgggatg attttatcga cctatgtctt
     1021 tcgcgtattc tcttatcttc ctttggtagc acaagggatt tggtggtttt catttctctt
     1081 ggatttgacc ttgattgctg gttttaccat caagtttgct tgtccagggc ggagggttca
     1141 tgccactcca agttggacgg ttctctatgt ggggatagca gtggctgcct tgacctatcc
     1201 tctggtaggt attatcgaaa ttgcctatgc gaccttgagt tttggttttc tcttgacctt
     1261 ctatctctat ccccttattt atagcgattt aaagaaacat ccactcccac tagccttgct
     1321 tggacaagaa ggaatctact gtgctccttt ctctctactc ttggcttctc tagttcgagt
     1381 aggaggaacc agcctgccga cttgggtctt gattgtcatg attttggctt ctcaatcctt
     1441 ctttttcttt gttttaactc gtctgcccaa cattttaaaa caaggttttc aaccagcctt
     1501 ctcagccctc accttcccaa ccattatcac agcgacctcg ctcaagatgg ctcagggaat
     1561 tttgaaactt ccatttctgg attacctggt attggctgaa accattatat gcctaactat
     1621 tttattcttt gtactaggtg cttatctgat ttggttacga aaaaggtcta gctagaaata
     1681 gctaaacctt attttttatg gtttgatgac ttcagctcca cccatgtatg gacgaagtgc
     1741 ttctgggatg gtcacagaac catcttcatt ttggtaattt tcaagaattg cagccactgt
     1801 acgtccaact gcaagtccag aaccgttcaa ggtatgaagg agtttcacct tgccatctgc
     1861 ttcatcacgg taacggattt gggcacgacg ggcttggaaa tcttctgtgt ttgaacagct
     1921 tgagatttca cggtaattgt tttgtgctgg aatccacact tccaagtcgt aagtcttcgt
     1981 agctgagaag cccatatctc cagtagagag agcaacgaca cggtatggaa ggttgagttt
     2041 ttgaagaatg ttttcagcgt tggctgtcat tttttccaat tcttcgtaag attcttctgg
     2101 tttggcaaat ttgaccattt caaccttgtg gaattggtgc aaacggatca agccacgcgt
     2161 atcacgaccg gcagaaccag cctcagaacg gaatgacgga ctcatagcag tgaagtagat
     2221 tggaagatct ttgccgtcta agatttcatc acggtagtag tttgtcagag gaacttcagc
     2281 agttggaatc aagacaaagt tggtatcgct gagttcaaaa gtatcttcct taaattttgg
     2341 atactgacca gtaccaaaca tagaatcatg gttgactatg taaggtgtga tgacttcagt
     2401 atagccttct tttccatgtt catccaacat aaagttgtag atagcacgtt ccaaacgagc
     2461 accgaggcct ttatagaaga ggaagcgagc gcctgttacc ttaccaccgc gttcccagtc
     2521 aaggatacca aggtcttcac cgagatccca gtgagcttta ggttcgaagt caaactcgcg
     2581 tggagtaccc caacggcgaa cttccacatt gtcgtcttcg tcagtcccaa caggaacgct
     2641 gtcagctggg atatttggaa gagtcgttgt aaattctgtc aatttagcat cgatttctgc
     2701 caattcagca tccaaggctt taacctcagc agatagattt tgcatggcag caatcttgtc
     2761 atctgtattt tccttgttgc gcttagcttg ggcaatctca gcagaaactg tgttacgttc
     2821 tgctttgaga gtttcaacct tgaccaagat gttacgacgt ttagcatcga tttctttcat
     2881 ttcattcaag acagcagcat ctacaccacg tgtagctaat ttttctgcga cagcttcaaa
     2941 atctgtacga atacgtttga tatctaacat aagaactcct ttatgaaaaa agcacgcctg
     3001 acaaaacgtt ggagtggcag ggccacggtt ccatccaact tcacaggtgt gcacttgatt
     3061 gtgtatgtaa ttgttattaa cggtagaatt tcacctatcc ctcctatctg ctcgcagtac
     3121 ccgcagactt tctgaaagaa gaagataacc tacttatccg ttgctatgat tatactaaag
     3181 tttctacttt tttgcaaata gatttttaaa tttttggcta attgtctgaa tcagggtcgg
     3241 aagtttgacg accttgtcat tgcctagttt tttgcgtgca attttgagaa tggcacctga
     3301 gtcttttgaa gcaaagagga attttccttt gtctgtaaag acttcgaagt ggcggctgat
     3361 tttgcgtcca gtgacattgg ctccaatctg attgatatgg ctccaaggaa tctggataaa
     3421 ttgttcgaca ttgacatctg ggtaaaattc caaagcctga tctccgacaa ggaatttccc
     3481 aactttccca gcgatagaga ggtaggaagt gcctgtcgta ctgaggagta ctgttttgtt
     3541 aagtgattgg gccatgctta gtcttcctta ctttctccaa aaaaggcatt gtagagggct
     3601 ttaattgctg ctttctcttg gtctttattg acaacaaaca taatagaaac ttcactagaa
     3661 ccttgagaca tcatctggat gttgattttg ttttcagata gagcgcgtgt cgcagtagca
     3721 gtcactccga tgtggctctt cattttttca ccaacaatca taatgataga aaggtcgtgt
     3781 tcgatttctg catgatctac tttagccttt tgaaccaact gacgcaggat ttcttcttcc
     3841 ttgatgggag ttagttggcg agaacggaga atgatagaaa gatcgtcgat acctgttggc
     3901 atatgttccc aaccgatgtt aagttcttcc aggatttgca gaaccttgcg gccaaatcca
     3961 acctcacggt tcatgaggta tttcgacatg ttaatgctga caaagcctga gtcaccagca
     4021 attcccacaa ctggaaattc atcattactg tgttttagaa cgatacgagt acctggatgg
     4081 tcagggttgt tggtattctt gataaccaga ggaatttttc cacggtaggc aggaagaaga
     4141 gcctcgtcat gaaggactga gaagcctgca taggccaact cgcgcatttc acggtaggtc
     4201 aactcaggaa tcgagtgtgg ttggtggata ataccagggt gggctgcaaa gataccatca
     4261 acgtccgtaa agttttcata gaggtcagct ttgacaccag cagcaatgat agaacctgta
     4321 atatcagaac ctccacgtga gaaagtacag atttgatttt ccttagtgac accaaagaaa
     4381 ccaggaatga caaggacttc attggtgttt gtcaattctt caatcttgtc ataacttgat
     4441 ggaatgatgc gagcgtgacc aggttcactt gtgaccacaa tcccagcttc tctagggtgc
     4501 atatagcgtg catcgatacc attttggtta aagtaggcag caatcaattt ggcattgtta
     4561 ttttcaccgg ctgctaggaa agtatcgtag agaaattcat tttcttcaat aggaagagtg
     4621 gccaaggcgt gaatactttt agaaattttt tctagcacag ctggttttag tcccaattca
     4681 ctaaccatag cagcatagcg gtcgataatc cagctttggc tcttgctaat atcgttaccc
     4741 gcaacatagt cgcggtagta tttaatcagg gcatccgtaa ccttagtatc ttcagcattg
     4801 cgtttaccag gcgcagaaac gactacaaaa cgacgctctg aatcgctttt gacgatgttt
     4861 aaaacttttt ctaattgact agcagaggca agagaactac ctccaaattt aacaactttc
     4921 ataagaactc ctaaagtaag tattttatac gattatagca gaaagaaagg cttttttcaa
     4981 tgaagaaaat aagatgcttt ctagcctaaa gcgagccttt cttatcggct gagacacttt
     5041 cagtcgattt tttcttgctt tctgctccta aaaaagatat actttgacag tgaaataatt
     5101 taacactcaa tgaaaatcaa agagcaaact aggaagctag ccgaagtcag ctcaaagcac
     5161 tgctttgagg ttgcagatgg aagctgacgc ggtttgaaga gattttcgaa gagtataaac
     5221 tgcttataaa ataaaaagga gccctgatgg aacacattat ttatcagctt gaagaggact
     5281 tggcaatcct taccttgaac cgtcctgagg tcgcaaatgg ttttcatatt cccatgtgtg
     5341 aggagatttt agaagctctg actttggcag aagaaaatcc agctgtgcat tttatcttaa
     5401 tcaatgccaa tggaaaggtc ttctcagttg ggggagattt ggtagagatg aagcgggcag
     5461 tggatgagga tgatattcca tcattgacaa aaatcgcaga gttggtcaat acgatttctt
     5521 ataaaatcaa gcaaatagcc aaacctgttt taatggaagt tgacggtgct gttgcaggtg
     5581 ccgcagcgaa tatggctgtt gcggcagatt tctgtttggc gacggataag gctaagttta
     5641 tccaagcctt tgttggtgtt ggtttggctc cagatgcagg gggaattcat ctcttgagcc
     5701 gcagtatcgg tgtgacgcgt gctgctcaat tagctatgac aggagaggct ttaacggcag
     5761 aaaaagccct agaatggggg ttggtttatc gcgtttctga agctgaaaaa cttgaaaaga
     5821 cgagagaaca gcttcttaaa aaattaagac gtgcttcaag taattcctat gctgccatta
     5881 agaagttggt ttgggagagc caatttaaag actggcaggg ttacgctact ttagaactga
     5941 acctacagaa atccttagct caaacagagg atttcaaaga aggagtgcga gctcattcgg
     6001 agagaagaag acctaaattt acaggaaaat aaaaaatact tgcatcattc tttgaatttt
     6061 gatataattc tttctgtcaa atgttttgat tgtaaaagtt ttttgaagaa ggagggatat
     6121 gaaaaatgga ctaccaacga atcaatgaat atttaacatc tatatttaac aatgtccttg
     6181 taattgagga agtgaacttg agaggtagtc gttttaagga tatctccatc aaagaaatgc
     6241 atacgattga tgtcatcggt aaggctccag acgtgactcc aagtcaagtg tcaaaagagt
     6301 tgatggtaac tcttggaact gttacgacaa gtttgaacaa tttagagcgt aagggctaca
     6361 ttgagcgagt tcggtcagaa caggatcgtc gtgtggtgca tctgcatttg acaaagaagg
     6421 gtcgcttgat tcataggctg cataaacgct tccacaaggc catggtagaa aaaattattg
     6481 atggcatgag cgaggaagaa attgctgtca tgggtaaagg tttgactaat ctttaccaat
     6541 ttttggagga tttgaaataa tggcttttgc aaaaataagt caggttgctc attatgtgcc
     6601 agagcaagtg gttacaaatc acgacttggc tcagattatg gataccaatg atgagtggat
     6661 ttcaagtcga acgggaatac gacaaaggca tatttcaaga acagaatcta ccagtgattt
     6721 ggctacagag gttgctaaga aactgatggc aaaagctgga ataacaggaa aagaactgga
     6781 ttttatcatc ctagctacca ttactccaga ttcgatgatg ccctctacag ctgctcgtgt
     6841 tcaagctaat attggcgcta ataaagcctt tgcttttgac ttaaccgcgg cttgcagtgg
     6901 atttgtattt gctctttcaa ctgctgaaaa gtttatcgct tctggtcgct ttcaaaaagg
     6961 cttggtgatt ggtagtgaaa ccctctctaa ggcagtcgat tggtcggatc gatcaacagc
     7021 tgtgttgttt ggagatggtg ctggtggtgt cttgttagaa gctagcgagc aagagcattt
     7081 cttagctgag agtctcaata gcgatggaag tcgcagcgag tgtttaactt atgggcattc
     7141 aggtttgcat tctccatttt cagatcaaga aagtgcagat tcgtttttga agatggatgg
     7201 acgcacagtc tttgattttg ccattcgaga tgtagccaag tctatcaagc agactattga
     7261 tgaatctcct atagaggtga cagacttgga ttatctgcta cttcatcaag ccaatgaccg
     7321 tattttggat aagatggcta gaaaaattgg tgttgaccga gccaaacttc cagccaatat
     7381 gatggaatat ggcaatacca gtgcagccag tatcccgatt ttactttcag agtgtgtaga
     7441 acaaggtctc atccctttag atggtagcca gactgttctt ctatcaggct tcggtggagg
     7501 cttgacctgg ggcacgctca ttcttacaat ttaggtaatc atgtggtgaa cacattgtta
     7561 taaaaaaact atttatttta aaggagtcct atcatggcag tatttgaaaa agtacaagaa
     7621 attatcgttg aagaacttgg aaaagacgca tcagaagtaa cacttgaatc aacttttgat
     7681 gatttggacg cagattcatt ggacttgttc caagtaatct cagaaatcga agatgctttt
     7741 gatatccaaa tcgaagcaga aaatgacttg aaaacagttg gtgacttggt tgcttacgtt
     7801 gaagagcaag caaaataagc agaatattag tagaaggagt agggagaaac ccactccctc
     7861 ttgtttaggt aatagtttga ctgtcaaatt atggtgaaat cgaactatta cgtaagcaag
     7921 gaaggttgga ggcactatga aaacgcgtat tacagaatta ttgaagattg actatcctat
     7981 tttccaagga gggatggcct gggttgctga tggtgatttg gcaggggctg tttccaaggc
     8041 tggaggatta ggaattatcg gtgggggaaa tgccccgaaa gaagttgtca aggccaatat
     8101 tgataaaatc aaatcattga ctgataaacc ctttggggtc aacatcatgc tcttatctcc
     8161 ctttgtggaa gatatcgtgg atctcgttat tgaagaaggt gttaaagttg tcacaacagg
     8221 agcaggaaat ccaagcaagt atatggaacg tttccatgaa gctgggataa tcgttattcc
     8281 tgttgttcct agtgtcgctt tagctaaacg catggaaaaa atcggtgcag acgctgttat
     8341 tgcagaagga atggaagctg gggggcatat cggtaaatta acaaccatga ccttggtgcg
     8401 acaggtagcc acagctatat ctattcctgt tattgctgca ggaggaattg cggatggtga
     8461 aggtgctgcg gctggcttta tgctaggtgc agaggctgta caggtgggga cacggtttgt
     8521 agttgcaaaa gagtcgaatg cccatccaaa ctacaaggag aaaattttaa aagcaaggga
     8581 tattgatact acgatttcag ctcagcactt tggtcatgct gttcgtgcta ttaaaaatca
     8641 gttgactaga gattttgaac tggctgaaaa agatgccttt aagcaggaag atcctgattt
     8701 agaaatcttt gaacaaatgg gagcaggtgc cctagccaaa gcagttgttc acggtgatgt
     8761 ggatggtggc tctgtcatgg caggtcaaat cgcagggctt gtttctaaag aagaaacagc
     8821 tgaagaaatc ctaaaagatt tgtattacgg agccgctaag aaaattcaag aagaagcctc
     8881 tcgctgggca ggagttgtaa gaaatgacta aaacagcctt tttatttgct ggtcaaggtg
     8941 cccagtatct agggatggga cgggatttct atgatcagta tccgattgtt aaagaaacga
     9001 ttgatcgagc gagtcaggtg ctaggttatg atttgcgtta tctcatcgat acggaagaag
     9061 acaaactcaa tcagacccgc tatacgcaac cagccattct agcgacttcg gttgctatct
     9121 accgtttatt gcaagaaaag ggctatcagc ctgatatggt tgctggtttg tctcttggag
     9181 aatactctgc cttggtggca agcggcgcct tggattttga agatgcggtt gccttggtag
     9241 ctaagcgtgg agcctatatg gaagaagcgg ctcctgctga ctctggcaag atggtagcag
     9301 ttctcaatac gccagtagag gtcattgaag aagcctgtca aaaagcttct gaacttggag
     9361 tggttactcc agccaactat aacacacctg cacaaatcgt cattgctgga gaagtggttg
     9421 cagttgatcg agcggttgaa cttttgcaag aagcaggtgc caaacgcttg attcctctta
     9481 aggtgtcagg tccctttcac accgctctcc ttgagccagc tagccagaaa ctagctgaaa
     9541 ctctagctca ggtaagtttt tcagatttta cttgtcccct agtcggcaat acagaagctg
     9601 ctgtgatgca aaaagaggac attgctcagc tcttgacgcg tcaggtcaag gaacccgttc
     9661 gtttctatga aagtattggg gtcatgcaag aagcaggcat aagcaacttt atcgagattg
     9721 gaccggggaa agtcttgtca ggttttgtta aaaaaattga tcaaactgct cacttagctc
     9781 atgtggaaga tcaagcgagt ttagtagcac ttttagaaaa atagactaaa ataagtagaa
     9841 gttttgaaag gaaaaaaatg aaactagaac ataaaaatat ctttattaca ggttcgagtc
     9901 gtggaattgg tcttgccatc gcccacaagt ttgctcaagc aggagccaac attgtcttaa
     9961 acagtcgtgg ggcaatctca gaagaattgc tcgctgagtt ttcaaactat ggtatcaagg
    10021 tggttcccat ttcaggagat gtatcagatt ttgcagacgc taagcgtatg attgatcaag
    10081 ctattgcaga actgggttca gtagatgttt tggtcaacaa tgcagggatt acccaagata
    10141 ctcttatgct caagatgaca gaagcagatt ttgaaaaagt gctcaaggtc aatctgactg
    10201 gtgcctttaa tatgacacaa tcagtcttga aaccgatgat gaaagccaga gaaggtgcta
    10261 tcattaatat gtctagtgtt gttggtttga tggggaatat tggtcaagct aactatgctg
    10321 cttctaaggc tggcttgatt ggctttacca agtctgtggc acgcgaggtc gctagtcgga
    10381 atatacgagt caatgtgatt gctccaggaa tgattgagtc tgatatgaca gctatcttat
    10441 cagataagat taaggaagct acactagctc agattccgat gaaagaattt gggcaggcag
    10501 agcaggttgc agatttgaca gtatttttag caggccaaga ttatctaact ggtcaagtga
    10561 ttgccattga tggtggctta agtatgtagc gaaagctaga ggtgaaaaga atgaaactaa
    10621 atcgagtagt ggtaacaggt tatggagtaa catctccaat cggaaataca ccagaagaat
    10681 tttggaatag tttagcaact gggaaaatcg gcattggtgg cattacaaaa tttgatcata
    10741 gtgactttga tgtgcataat gcggcagaaa tccaagattt tccgttcgat aaatactttg
    10801 taaaaaaaga taccaaccgt tttgataact attctttata tgccttgtat gcagcccaag
    10861 aggctgtaaa tcatgccaat cttgatgtag aggctcttaa tagggatcgt tttggtgtta
    10921 tcgttgcatc tggtattggt ggaatcaagg aaattgaaga tcaggtactt cgccttcatg
    10981 aaaaaggacc caaacgtgtc aaaccaatga ctcttccaaa agctttacca aatatggctt
    11041 ctgggaatgt agccatgcgt tttggtgcaa acggtgtttg taaatctatc aatactgcct
    11101 gctcttcatc aaatgatgcg attggggatg ccttccgctc cattaagttt ggtttccaag
    11161 atgtgatgtt ggtgggagga acagaagctt ctatcacacc ttttgccatc gctggtttcc
    11221 aagccttaac agctctctct actacagagg atccaactcg tgcttcgatc ccatttgata
    11281 aggatcgcaa tgggtttgtt atgggtgaag gttcagggat gttggttcta gaaagccttg
    11341 aacacgctga aaaacgtgga gctactatcc tggctgaagt ggttggttac ggaaatactt
    11401 gtgatgccta ccacatgact tctccacatc cagaaggtca gggagctatc aaggccatca
    11461 aactagcctt ggaagaagct gagatttctc cagagcaagt agcctatgtc aatgctcacg
    11521 gaacgtcaac tcctgccaat gaaaaaggag aaagtggagc tatcgtagct gttcttggta
    11581 aggaagtacc tgtatcatca accaagtctt ttacaggaca tttactgggg gctgcgggtg
    11641 cagtagaagc tatcgtcacc atcgaagcta tgcgtcataa ctttgtacca atgacagctg
    11701 ggacaagtga agtatcagat tatatcgaag ctaatgtcgt ttatggacaa ggcttggaga
    11761 aagaaattcc atacgctatt tcaaatactt ttggttttgg aggccacaat gcagttcttg
    11821 ctttcaaacg ttgggagaat agataagtat gaatttaaac gatattaaag acttgatgac
    11881 tcaatttgac cagtcaagtt tgagagaatt ttcttataaa aatgggacgg atgagttgca
    11941 gtttagcaag aatgaagcaa gacctgtgcc tgaagttgca actcaagtcg ctccagcacc
    12001 cgttctagca acaccgagtc cagtagctcc tacatctgct ccagcagaga ctgtagcaga
    12061 agaagttcca gctccagctg aagcaagtgt ggctagtgag ggaaatcttg tagagagtcc
    12121 acttgttgga gtggtttact tggctgctgg accagataaa cctgccttcg ttacagttgg
    12181 tgatagtgtc aaaaaaggtc aaacattggt aattatcgaa gccatgaaag tcatgaatga
    12241 aatcccagct cctaaggatg gtgtggtaac ggaaattctc gtctctaacg aagaaatggt
    12301 tgagtttggt aaaggattgg tacgtatcaa atgatcgata ttcaaggaat caaagaagct
    12361 cttccccacc gttatcctat gcttctagtg gaccgtgtct tggaagtgag cgaggatacc
    12421 attgttgcta tcaaaaatgt gaccattaac gaacctttct ttaacggcca ctttcctcaa
    12481 tacccagtta tgccaggtgt tctgattatg gaagccttgg cgcaaactgc tggtgtgttg
    12541 gagttatcaa aacctgaaaa taaaggaaaa ctggtctttt acgctggtat ggacaaggtt
    12601 aagttcaaga agcaagttgt accaggcgac caattggtta tgacagcgac ttttgtaaaa
    12661 cgtcgtggca ccatagctgt ggttgaagca aaggctgaag tggatggcaa gcttgcagcc
    12721 agtggtatcc ttacttttgc aattgggaac taaggaggtt ctccatgttt cgaaaaattt
    12781 taattgccaa tcgtggtgaa attgcggttc gtattatccg tgcggcacgt gaattgggga
    12841 ttgcgacggt agcggtttat tcaactgctg ataaggaagc tcttcatacg cttttggcag
    12901 atgaagcagt ttgtattggt cctggcaagg caacagagtc ttatctcaat attaatgcag
    12961 ttctatcagc tgcagtcttg actgaggcag aagctattca ccctggtttt ggatttctca
    13021 gtgaaaattc caaatttgcg accatgtgtg aagaagtagg tatcaagttt atcggtccat
    13081 ctggtcatgt tatggatatg atgggggata agatcaatgc gcgtgctcag atgattaaag
    13141 caggtgtgcc tgttatacca ggttcagatg gagaagtgca taactctgaa gaagctttga
    13201 ttgttgctga aaaaattggc tatcctgtta tgctcaaggc ttcagcaggt ggaggtggta
    13261 aagggattcg taaggttgaa aaaccagatg acctcgtttc tgcctttgaa actgcctcta
    13321 gtgaggccaa ggccaattat ggcaatggtg ccatgtacat agaacgggtt atctatccag
    13381 ctcggcacat tgaggttcaa atcctaggtg atgagcatgg acatgtgatt cacttgggtg
    13441 aacgggattg ttctcttcaa aggaataacc aaaaggtttt ggaagaaagt ccctcgattg
    13501 caatcggaaa aacgctgcgt catgaaatag gtgctgctgc tgttcgagcg gcagagtttg
    13561 ttggctatga gaatgcagga accattgaat ttcttcttga tgaagcaagt agcaatttct
    13621 atttcatgga gatgaatact cgtgttcagg tagaacatcc agtaacagag tttgtttcag
    13681 gtgttgatat cgttaaggaa cagatttgca ttgcggcagg tcagcctttg tctgttaagc
    13741 aagaagatat tgtcctacgt ggtcatgcca tcgagtgtcg tatcaatgca gaaaacccag
    13801 cctttaactt tgctccaagt ccaggtaaga ttactaatct ctatctgcca agtggtggag
    13861 ttggcttgcg cgtggattca gcagtttatc caggttatac cattccgcct tattatgata
    13921 gtatgattgc caaaatcata gtacacggcg aaaatcgttt tgacgccttg atgaaaatgc
    13981 aacgtgccct ctatgaatta gagattgaag gagtgcagac caatgcagat ttccagcttg
    14041 acctcatttc agatcgcaat gtcattgctg gggattatga tacttccttc ttgatggaaa
    14101 ccttcttacc taaatatcaa gaaaaagaat aaaatgactt taagagttta aaccgaaaag
    14161 gggaatcaat ggctctattt agtaaaaaag ataagtatat tcgaatcaat cccaatcgtt
    14221 cggttaggga aaaacctcaa gctaagccag aggttccaga tgaattattt tcccagtgtc
    14281 caggctgtaa gcataccatc tatcagaagg atctgggaag tgaacgtatc tgtccgcact
    14341 gtagctatac ctttcgtatt tctgcccaag aacgcttggc tttgacgatt gatatgggaa
    14401 ccttcaaaga attgtttaca gggattgaaa gcaaggatcc cttgcatttc cctggttacc
    14461 aaaagaaact ggcatctatg cgtgaaaaaa caggtctgca tgaagccgtt gtgacaggaa
    14521 ctgctcttat taaaggtcag actgtggctc ttgggattat ggattctaac tttatcatgg
    14581 cttctatggg tacggttgta ggtgaaaaaa tcactcgttt gtttgagtat gcgactgtcg
    14641 aaaaattgcc agttgtccta ttcacagcct ctggtggagc ccgtatgcag gaaggaatca
    14701 tgagtctcat gcagatggct aagatctctg cggcggttaa acgccattca aatgctggtc
    14761 tcttttacct gaccattttg acagatccaa cgactggtgg tgtgacagct tctttcgcta
    14821 tggaaggcga tatcattctg gctgaaccac agagcttggt tggttttgct gggcgtcgtg
    14881 tgattgaaaa tacggttcgt gaaagcttgc ctgaggattt ccaaaaggca gaattcctat
    14941 tagaacatgg ctttgtggat gctattgtca aaagaagaga cttaccagat acgattgcta
    15001 gcctagtcag attgcatgga gggagtccta gatgaatatt gcaaaaatag tcagagaagc
    15061 gcgtgagcag agtcgcttga caaccttgga ctttgcgaca ggcatttttg atgaatttat
    15121 ccaattacat ggtgaccgtt cttttcgtga tgatggtgca gttgttggtg gtattggttg
    15181 gcttggagac caagctgtaa cagttgttgg tatccaaaaa ggcaagagtt tgcaagacaa
    15241 cctcaaacgg aattttggcc aaccacatcc agaaggctac cgaaaggcac tgcggttgat
    15301 gaaacaggct gagaaatttg gccgtccagt tgtgaccttt attaatacag caggtgctta
    15361 tcctggtgtc ggagcagaag aacgtggtca aggggaagct atcgctcgca atctcatgga
    15421 aatgagtgac ctgaaagttc ctattatcgc cattattatc ggtgaaggtg gttcaggcgg
    15481 ggctctggct ctagctgtcg cggaccgtgt ctggatgctg gaaaattcta tctatgccat
    15541 tctcagtcca gaaggctttg cttccatttt atggaaggac ggtactcgcg ccatggaagc
    15601 agcagaactg atgaaaatca cttcgcatga actgttagaa atggacgtgg tggataaggt
    15661 gatttctgaa gtaggacttt ctagtaaaga actgattaag agtgtcaaaa aagaactcca
    15721 aacggagctg gctagacttt cacaaaaacc gctagaagag ttgctggaag aacgctatca
    15781 acgatttaga aaatactaag aatgactaaa tgagactaga aaggtctcat tttttatgga
    15841 aaaatgcaat tcaggttgaa aaattgacag ttgaggagat agactataaa atagacaaaa
    15901 aaagaaatat actataaatg tgagcctaca acaatctatt ttaagaaggt acttgcaatg
    15961 acaggtacaa atacatttac agttctttca actgaggact tggagcaaac ttcaggtggt
    16021 cttgctgttt gggaagatgg atatagtaga tggttatatt atagagaatt tgctccctat
    16081 atgaggcaag gggcacttaa ttcttatata gatgcttgga agtacggctt ccgagcaggg
    16141 taatctttca aaaaagtacg atgagaaaca caaaaaaact aagacaattt ggaatatttt
    16201 tgcttattat tttactatct acctatttac cacaaacaat tggattgtat gtgacgatca
    16261 ttttagggtt aggagctgat gtctattctc ttattttaac aatggtatta gtgggaagtt
    16321 tccttctatt gatttggagg ttaaaaaaga aaaagatgct ctttatcttt gaaaaaaaga
    16381 gctggaactg gtcatttgtc ttctacttat ttgcaacgta tgtggtttat cagattctcg
    16441 gtaattttta ggcacgctat gctcatttga ttaaccatag gaatattcat gatgagtatt
    16501 ttaccgtggt tctttcaaat ggacaaccaa cctttttatc gactatctta tcttttgtcc
    16561 tgcttgtaat catcggtcct gtttttgagg agactcttga tagagggtat ttgatgaata
    16621 ccttctttcc taagtcaaag tactatttag atgtcatctt atcaggcctt atctttggga
    16681 ttagccattt gatactatct cacagagatc caattagttt attatattat agtctgattg
    16741 gtttcttttt tgcccttgtt tatcgttcta ccgacaatct gagattaacg attctctgcc
    16801 atagtttttt taactttctc aatcatgcaa aacctatctg gatttttgtt tacaattata
    16861 tctattatca tttttttaga tagtgggagg caaagaaaaa gtgtttgata tctatcaaac
    16921 actttttctt tttattgttc ttctgttaca aactggctga gcagtccatt gataaaacgg
    16981 gcagattttt gatcggagaa gtcctttgca agctcgatag cttcattaac agcaaccagc
    17041 tgaggagtgt caaatgaagt gatttcaaaa actcccaagc gaaggaggtt tctctccacg
    17101 agcgttaagc gttcaatggt ccaacctact tttaaatgct gagtgatttg cttatctagt
    17161 tcttcctttt tagcttgaac accagaaacg aggtctatca aaaaggctgg aagttgtaca
    17221 tccgtatctt cacgatcatg agtataggcg aaacgacaag cagtttcgac attcgtaccg
    17281 aactcaaggc tcatgagagc ttgaaaagcg catttacgga gttggcgtct agattctaat
    17341 agtggactag tcattgagga agtcctcgtc aaatagatct ttcaattctg gttttggtgt
    17401 tttatctggg acgatacctg caacgtgaat attgatagca gcgagttcta catcagccat
    17461 attacggacg gcatctttga cagctttctg gatagcaacc gctaccttag gaacttttac
    17521 tccgtactca aggtagagat agatatctgc tgtgagttct tcgtccacgt ttttaagata
    17581 aacgccacgg ccgagtgaaa gttttgaaag ggtatcagac actgatctgt ttgaaaaaga
    17641 gtgaacaccc tctacctttg cagtagcgat agcaatgatt ttttcaagta cacgtggggc
    17701 gataacgatt tcgccaagtt gttcttcaat tcccatagaa tgacctcttt ctagagatta
    17761 ggcacgagaa acgtaagttc cttctgcagt gttgataacg agtttttgtc ctgcttcgat
    17821 gaagtctgga acgtttacga caagtccagt ttccatcgtt gctggtttgc cagaacctgt
    17881 aacagtagca cctttgatag atggttgagt ttcagcaact gtcaactcaa cagtagtagg
    17941 aacggtgaca ccgatcactt cagttccgta gaattggatt ttcacatcag agttttcaag
    18001 gatataaagc aattcgtttt caacattgac tacagggatt tcgtactggt cgtaagtttc
    18061 tgtattcatg aagtatgctg tgtcatccat tttgtacaag tattgagctg ggacagtctc
    18121 gataatagct tgttcaaatt tttcctctgg acggtagctt gtgtcaaatg tagaaccagt
    18181 acggacatca cgcaatttca tacgcatgat cgtgtttcct ttacctggct tgtggtgact
    18241 agcttccaaa acgcgaatca atttgccgtc agctgtttca aaggtcatac cagcttttaa
    18301 tttacttgct tcaatcatgt ttttacctct ttaataaata ttattactct tcattttacc
    18361 ataaaatact ctggaaataa agccaaaata gttattggga gttggtagag aagaaaaaac
    18421 caaccttagg gctggttttg ttctgtctag ttttctttca acttcgccaa ttcctgtgca
    18481 agtagtttaa gggcaacttg tgggttggct tggcctttgg ttgccttcat aaggaatcct
    18541 gtaaaggcct tgtcggcgtt acgtttgcct gacttgaagt cggcaacagc agcttcgtta
    18601 tcggcaaaga cttggtggat gattgggatc aagatagctg gatctgaaat ttgaaccata
    18661 cctgcttttt ccacgtattc acgcgcgcca ccgccatttt tagctagatg gacaaagact
    18721 ttcttggcaa tcttagatga aatagtaccg tcttcgatga tggtaatcat ttcaaccaag
    18781 ttttctggtg tcaattcgat ttgttccagt gttttacctt cagcattcaa gaactgagcg
    18841 acttcccctt ggagccagtt agagacttgt ttggcatcac cacctagggc aacagctttt
    18901 tcaaagaagt cagaagtgac tttattagca gtcaactgac tagcatcgta gtctgataaa
    18961 ccaaggtcag atacataacg cgcacgacgt tcttttggaa actctggcaa ctcagtccgc
    19021 atttcctcaa tccactcgtc agaaatttca aagaggggta ggtctggttc tgggaagtag
    19081 cggtagtcag cagccccttc cttgacacgc atgaggatgg ttgttttgtt agcttcatcg
    19141 taacggcgtg tttcttggcg gatttgacca cctgagcgaa gaatttcagc ctggcgttgg
    19201 acttcgtatt caagaccttt acgaacgttt gagaaggagt tgaggttctt caattcagtc
    19261 ttggtaccga atttttcttg accataaggg cgaagggaga tgttggcatc cacacgcata
    19321 gaaccttctt ccatcttaac gtcagaaatg ccagcgtact ggataacttc cttgagggct
    19381 gtcagataag cataggcctc ctctggcgaa cgcatatcgg cttcagatac aatctcaatc
    19441 aaaggtaccc cttggcggtt aaggtcaacg taagagtagc cgtctgtacc gtgggtgttt
    19501 ttaccagcgt cttcctctaa gtgggcgcgt tcgataccaa ttttcttggt cgtaccgtct
    19561 tctagctcga cttcaatcca gccgttataa ccgattggct catcaaactg agaaatttgg
    19621 taggctttgg gattatcagg gtagaagtag ttcttgcggt caaagtgcat ttttttgtgg
    19681 aggtccatgt tgagggcaag agcagccttg ataccggcat caacaacccc tttattgaga
    19741 actggtagaa ctcctgggaa agaccagtca atcacgttag tgttggcatt ttggtcattt
    19801 ccaaagtggg cagaagtagg tgagaagatt tttgaattgg tgttgagctc tacgtggact
    19861 tcaagtccga tgactgtttc aaagttcatt agttgtcacc tccaaaaatc acgggttgtt
    19921 gtttgtggta gtctgttgtt gcttcaaaag cagcagcagc ttggtaaatg gtttcctcag
    19981 agtacttggg accaatcaat tggagtccga caggtagacc ttgagagaat ccagcaggaa
    20041 tcgaaattcc aggcagtcct gccaagttta caggtatggt caagaggtcg gctaagtaca
    20101 tggcaactgg gtcatggttg agagaatcca agtcataggc aacacttgga gcagttggac
    20161 ccaaaatcaa atcgtaatcc gcgaagactt tttcgaaatc ttgaatgata agtgtacgga
    20221 cctgtccagc cttcttgtag taggcatcgt agtaacctga tgaaagactg aaagtaccca
    20281 gcatgatacg gcgcttcact tcttcaccaa aaccttggct acggctgttt acatagattt
    20341 catcaagatt agtcgcatct tctgcgcggt agccgtaacg gataccgtca aaacgttgca
    20401 agtttgatga agcttctgat gaagcgatga tgtaataaac ggcaacaccg tatttagagt
    20461 gaggaaggct gacttcttcg acgatagcac ccaatttttc aaagtgtttg gccgcgttta
    20521 agattgtttc cttaacctct ggatcaattc cttcgcctag gtattcctta ggcaaagcaa
    20581 ttttcatgcc cttaatgtct tggccgattt ttgaagtaaa gtcggcgatg cggacaggag
    20641 cagaagtaga gtctttagta tcttcgctgg caatagcgtt gagcaagagg gcattttcct
    20701 taacagtagg agcaaaaggt ccaatctggt ctaatgagct accaaaggca atgagaccga
    20761 aacgtgaaac tgttccgtag gttggtttga gaccaacgat tccgttgaag gcagcaggtt
    20821 ggcggatgga accaccagta tcagaaccaa gtgacaagcg aacttgtcct gaggctacag
    20881 ctgcggcaga accacttgat gacccaccag gaaccttgct gtggtcccaa gcgtttttag
    20941 ttgctccgta gtgtgaagtt tcaccggaac cacccatagc aaattcgtcc atgttggtct
    21001 ttccaacgac aatcatgcct ttggtttttg cattggcaac agctgtcgca tcaaagattg
    21061 gctcatagtt gtagagcatt tttgaggcag cagttgtgag aataccgtct gtagagatgt
    21121 tatccttaac agcaagtgga attcctgaaa ggacattgtt agcatcaatt ccagcttcat
    21181 caatggcttt agcttgaaca agagcttgct cctcagcgat ggtgacaaat gaattgaggg
    21241 cttcctcacg agacttgata ttttcaagtg ttgcttgggt caattctgtt gcagaaattt
    21301 ccttagagac aaggagattg tgcaactctt caatagtttt attgttaaaa gtcattaggc
    21361 atctcctcca tcgtctagga tagctggcac cttgatatag tagttgtctt gttcaggtac
    21421 gtttttaaac aagcgatcac ggtctgttcc ttcttcggcc acatcagggc ggagtacagt
    21481 cttgcggtca gccatagtcg tagtaggtgc gacaccagtt gtgtcaactt cgcccagcaa
    21541 ttcaaccatg tcaacaatct tagacaaggt ggtcgcaaag gcagcagttt cttcttcaga
    21601 gaatcttaat tttgaaagat tggcaacgtg tgttacctct tcttgcgtaa ttttcatctt
    21661 gcatcctttc gtgaaatgat gatttttagt ctgttctatt ttaccatatt ttcctataaa
    21721 taagggaggg ggaagtgaaa agaaatagaa attgaaaaaa atgctaaaat ccgatataat
    21781 ggaatgttga aaggaatggt aaaatccaat gtaaaaatca ttctcagcta ttgaaacaag
    21841 aaaaatagag aatcaaagaa agagaactta tgaatattca agaagaaatt aagaaacgtc
    21901 gtacctttgc cattatctcc cacccggacg cggggaaaac aaccatcact gagcagttac
    21961 tctactttgg gggtgagatt cgtgaggctg gtacggtaaa agggaagaaa acagggactt
    22021 ttgctaaatc tgactggatg gatatcgaga agcaacgtgg gatttctgtt acttcatctg
    22081 ttatgcaatt tgactacgac ggcaagcgcg tgaatatctt agacacgcca gggcacgagg
    22141 acttctcaga agatacctat cgtaccttga tggcggtgga tgctgcggtc atggtcgtgg
    22201 actctgccaa ggggatcgag gctcaaacca agaaattgtt tgaggttgtg aaacatcgtg
    22261 gcattccagt ttttaccttt atgaacaagc tggaccgtga cggtcgtgag cctttggatc
    22321 tcttgcaaga attggaagaa atcttgggca ttgctagcta ccctatgaac tggcctatcg
    22381 ggatggggaa agcctttgag ggcttgtatg acctctataa ccaacgttta gagctttaca
    22441 aaggggatga gcgttttgcc agtctagaag acggggacaa actctttggt agcaatcctt
    22501 tctacgagca agtcaaggat gacattgagc ttttaaatga agctgggaat gagttttcag
    22561 aggaagctat tctggctgga gaattgacgc ctgtcttttt cggttcagcc ctgacaaact
    22621 ttggtgtgca gaccttcctt gaaatcttcc tcaagtttgc tccagaacca catggtcaca
    22681 agaaaacaga cggtgaaatt gtggatcctt atgacaagga tttctcaggc tttgtcttta
    22741 aaatccaagc caacatggat cctcgtcacc gtgaccgtat tgcctttgtc cgtatcgtat
    22801 caggcgaatt tgagcgtggc atgagtgtca atctccctcg tactggtaag ggtgccaaac
    22861 tatctaatgt tacccagttt atggcggaga gtcgtgagaa tgtgaccaat gccgtatcag
    22921 gtgatattat cggggtttac gataccggta cttatcaggt tggggatacc ttgacggttg
    22981 gaaaaaacaa gtttgaattt gaaccactgc caacctttac tcctgaaatt ttcatgaaag
    23041 tttctgctaa gaatgttatg aagcaaaaat ccttccacaa ggggattgag caattggtgc
    23101 aagaaggagc cgttcagctt tataagaatt accaaacagg tgagtacatg ctgggagctg
    23161 ttggtcaact ccagtttgaa gtctttaaac accgtatgga aggcgaatac aatgcggaag
    23221 tggtcatgag cccaatgggt aaaaagaccg ttcgttggat caagcctgag gacttggatg
    23281 aacggatgtc gtcaagtcgc aatatcttag ccaaagaccg ttttgaccaa ccagtctttc
    23341 tctttgaaaa tgactttgcc ctccgctggt ttgcggacaa gtatccagac gtagagttag
    23401 aggagaagat gtgactcagt accaacaatt ggaactaaag ttccaaattg ttggactcta
    23461 gtcgctattt ggcgaactag ctaactgcct tactaactac gtttggcaaa tgaaatcgat
    23521 ttgtaaaact ccgtgtcgta atgtaaaaat tgtaaaccgt aatgggactt catctaaaat
    23581 ctccactggt atggttttac gaacgaatgg gcgaaaataa tggttacgta gaaacatatg
    23641 atgggaataa aaagaaatga gataaagagt gaaagaagag aaaaaagcga ttgtcttagg
    23701 tgcagataat gcttatatgg ataaagttga aacaacaata aaatctcttt gtgttcatct
    23761 attataatct taagttttat gtttttaatg atgaccttcc gcgagaatgg ttccaattga
    23821 tggaaaaaag actagagact ttgaatagtg agattgtgaa tgtttagatt gacagtagta
    23881 ttctgaaagg ttatcgtctc ccctttgagg gactttcata tgcggctttt ttaggtattt
    23941 tattccaaag tatgtaagcg agtctcgtgt actgtatttg gatttagata ttgttgtaag
    24001 aaaatcgata gatgagctgt gggatttgga cttgacggct atacccttag ctgcagttag
    24061 agatgatttt tatacacata attttaattc tggtgttctg ttaatcaaca atggcatgtg
    24121 gagagcagaa aatgtcacgc aagacctgat ttaagacaca aataaaaaat cttccaattc
    24181 ccttgcaaaa aacaagaaag tgtgataaca tagtacggta tgtggtgcta gcacatccgc
    24241 tatattagat ctaataggag gaaaacacaa tggctaaagt atgttacttt acaggtcgta
    24301 agactgtatc aggaaacaac cgttcacacg cgatgaacca aacaaaacgt gccgtaaaac
    24361 caaaccttca aaaagttact gttcttatcg atggtaaacc taaaaaagtt tgggcttcag
    24421 ctcgtgcttt gaaatcaggt aaagttgaac gcgtttaata ataaaagcaa aggagacctt
    24481 agggtctttt ttcttttatt gtgggtataa catcgtttga aaaatagagt aaatatccgc
    24541 tgatacatca ttctgctttt acacttgggc tgaaatatga taaaatagag tatcaactag
    24601 ttgaggtaaa aaggatgact gtaaaaatta atacaaaaga tggtcaaatc gaactaacag
    24661 atgaagtgat tgcaaccgtc gtaggtggag cagcaactga gatttttggt gtggtcggta
    24721 tggctagtaa aaatgccctc aaagataatt tccaagccct gctaggtaag gaaaattatt
    24781 ctaaaggtgt cgtcgtaaag gcagccgaag atggcagtat tgcagttgat gtatataccg
    24841 tgttaagcta cggaacaaag attagtgaag tgtcaaaaaa cattcaagag cgtgttgcct
    24901 ttagcttgga aaaccagctt ggaattactg ctcagactgt aaatgtctac attcaaaata
    24961 tcaaagttgt aggagaataa ccgtgtcaaa aattactact agcttatttc aagaaatggt
    25021 gcaggctgca tcaactcgct tgaataagca agctgaatat gtcaattcat taaacgtctt
    25081 tccagttcca gatggagata ctgggacaaa tatgggaatg accattgaaa atggtgctaa
    25141 agaagttgca gacaagccag cttctacagt tggagaagta gcgagcattc ttgctaaagg
    25201 gcttttgatg ggtgcgcgtg ggaactcagg agtgattacg tctcagcttt tccgtggatt
    25261 ttcccaagct atcaaggata aagacgagtt aacaggtcaa gacttggctc tggccttcca
    25321 atcaggtgtg gaagttgcct ataaggcagt gatgaaacct gttgaaggaa cgattttgac
    25381 agtttctcgt ggagctgcta tcggtgctaa gaaaaaagct gagcaaacag atgatactgt
    25441 tgaagtcatg cgcgcagcct tggaaggtgc taaaacagct ctagctaaaa caccagacat
    25501 gcttccagta ttgaaagaag ttggcgttgt ggactcaggt ggtcaaggat tggtcttcat
    25561 ctacgaaggt ttcctttcag cccttactgg cgaatatatt gcatctgagg actttgtagc
    25621 gactcctgcc aacatgagtg agatgatcaa tgtagagcat cataagtctg tagctggtca
    25681 cgtagcgact gaggacatca cgtttggtta ctgtactgaa atcatggtag ctcttaagca
    25741 aggtccaacc tatgctaaag attttgacta cgatgaattc cgtaactact tggatgagct
    25801 tggggattct ctcctcgttg ttaacgatga tgaaattgtc aaagtccatg ttcatacaga
    25861 agatccagga cttgttatgc aagaaggtct caaatatggt agcttggtca aggtaaaagt
    25921 tgacaatatg cgtaaccaac acgaagcaca ggttgagaaa gaagctactc aagttatcaa
    25981 gtcggctgaa gaaaaagagt atgctttgat tgctgtggtg gctggtaaag gtctagcaga
    26041 tatcttctgt tctcaaggcg tggattatgt tatcgaaggc ggtcaaacca tgaacccttc
    26101 aacagaagac tttatcaagg ctgttgaaca ggtcaatgcc cgtaacatca tcttcttgcc
    26161 aaacaacaag aacatcttca tggcagctca atctgcggca gaagttttgg agcaaccagc
    26221 agtagtggta gaggctcgca ctcttcctca aggtatgaca agtcttcttg cctttgatcc
    26281 aagcaagtcc attgaagaaa accaagagcg tatgacagct gctcttagag atgtcgttag
    26341 cggaagcgtc acaacagccg tgcgtgatac aacgatcgat ggcttagaaa tccatgaaaa
    26401 cgataatcta ggtatggtgg atggaaaaat tcttgtgtca aaccctgata tgcaccaaac
    26461 attgactgaa accttgaaac atatgttgga tgaagacagt gaaatcgtaa ccttctatgt
    26521 cggtgaagac ggaagcgaag aacttgccaa tgaaatcgct caagaaatcg tagaagaatt
    26581 cgaagacgtt gaagtcgaga ttcaccaagg tcaacaacct gtttacccat acctatttag
    26641 tgtggaataa gat
//
LOCUS       DAVKGU010000020        24115 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKGU010000020 DAVKGU010000000
VERSION     DAVKGU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24115
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.020"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(197..355)
                     /locus_tag="VW077_000819"
     CDS             complement(197..355)
                     /locus_tag="VW077_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454872.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(365..1675)
                     /gene="obgE"
                     /locus_tag="VW077_000820"
     CDS             complement(365..1675)
                     /gene="obgE"
                     /locus_tag="VW077_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9454873.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVLVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLVTLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            complement(1739..1873)
                     /locus_tag="VW077_000821"
     CDS             complement(1739..1873)
                     /locus_tag="VW077_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9454874.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            complement(1932..2108)
                     /locus_tag="VW077_000822"
     CDS             complement(1932..2108)
                     /locus_tag="VW077_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9454875.1"
                     /translation="MRSGRDKKIAGVCAGVAYYLDMDPTIVRVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            complement(2139..3464)
                     /locus_tag="VW077_000823"
     CDS             complement(2139..3464)
                     /locus_tag="VW077_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9454876.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            complement(3480..4523)
                     /gene="cpoA"
                     /locus_tag="VW077_000824"
     CDS             complement(3480..4523)
                     /gene="cpoA"
                     /locus_tag="VW077_000824"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9454877.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEENLLQIW
                     LDFYEKQAALGRK"
     gene            complement(4638..4967)
                     /locus_tag="VW077_000825"
     CDS             complement(4638..4967)
                     /locus_tag="VW077_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9454878.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            complement(4981..6090)
                     /gene="rpoD"
                     /locus_tag="VW077_000826"
     CDS             complement(4981..6090)
                     /gene="rpoD"
                     /locus_tag="VW077_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9454879.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            complement(6093..7853)
                     /gene="dnaG"
                     /locus_tag="VW077_000827"
     CDS             complement(6093..7853)
                     /gene="dnaG"
                     /locus_tag="VW077_000827"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9454880.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQDLYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            complement(7943..8701)
                     /locus_tag="VW077_000828"
     CDS             complement(7943..8701)
                     /locus_tag="VW077_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454881.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            complement(8698..9564)
                     /locus_tag="VW077_000829"
     CDS             complement(8698..9564)
                     /locus_tag="VW077_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454882.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            complement(9633..10637)
                     /locus_tag="VW077_000830"
     CDS             complement(9633..10637)
                     /locus_tag="VW077_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9454883.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEVQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     misc_binding    complement(10750..10967)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11050..13746)
                     /gene="ppc"
                     /locus_tag="VW077_000831"
     CDS             complement(11050..13746)
                     /gene="ppc"
                     /locus_tag="VW077_000831"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9454884.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(13766..14995)
                     /locus_tag="VW077_000832"
     CDS             complement(13766..14995)
                     /locus_tag="VW077_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9454885.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGLSVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(15123..15452)
                     /locus_tag="VW077_000833"
                     /pseudo
     CDS             complement(15123..15452)
                     /locus_tag="VW077_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(15557..15805)
                     /locus_tag="VW077_000834"
                     /pseudo
     CDS             complement(15557..15805)
                     /locus_tag="VW077_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            complement(16086..16673)
                     /locus_tag="VW077_000835"
     CDS             complement(16086..16673)
                     /locus_tag="VW077_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEU9454886.1"
                     /translation="MGGNPPIKNYTIVNKIVSSRKIERIVIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYVNQEFKSYIDHM
                     LNKEAKIIGFSAGALLLGEKVYVSPNVNSDYQIKIKNGLGLFSQFLISVHYDSWNDKA
                     NKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            complement(16778..18571)
                     /locus_tag="VW077_000836"
     CDS             complement(16778..18571)
                     /locus_tag="VW077_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9454887.1"
                     /translation="MKKNNVEIIKADSLVRRRGDNVERHLKRVAAYCRVSSDSEDQKN
                     SYDSQVRHYKEYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCANGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELVGFQGCLGYDYDVETKQISINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLEHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSN
                     FGEEDKYYIKDNHEPIISKEDFEKAQEIRLRRAGNKKTAANVNGKRERYSKMYAFSSM
                     LECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEEIAIEGAFLEAY
                     RQVYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRILLKKEENLVNLRLEGKVS
                     DSIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDRA
                     VFESIVEKIIVGGVNSNGEIDPAMLTIIFKTGEIQNKDGKQFKSKRKNAKLETDKLCP
                     QNSDEDKKLYSQGTDNTRGVCSVAGSILASQ"
     gene            complement(18738..18890)
                     /locus_tag="VW077_000837"
     CDS             complement(18738..18890)
                     /locus_tag="VW077_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454888.1"
                     /translation="MTTNKDNDTSERSGCQRKRTAGEMPMNDLTSHTHGGNYTCYKEE
                     KVWNNQ"
     gene            complement(19021..19224)
                     /locus_tag="VW077_000838"
     CDS             complement(19021..19224)
                     /locus_tag="VW077_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9454889.1"
                     /translation="MYIRLKNLREDKDLSQCQIAQYLHCSQSAYSRIESGKQDIPTSF
                     LKQLAKLYQVTTDYILEMDKFSC"
     gene            19726..20921
                     /locus_tag="VW077_000839"
                     /pseudo
     CDS             19726..20921
                     /locus_tag="VW077_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(21300..22085)
                     /locus_tag="VW077_000840"
                     /pseudo
     CDS             complement(21300..22085)
                     /locus_tag="VW077_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(22087..22906)
                     /locus_tag="VW077_000841"
                     /pseudo
     CDS             complement(22087..22906)
                     /locus_tag="VW077_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
     gene            complement(22908..23900)
                     /locus_tag="VW077_000842"
     CDS             complement(22908..23900)
                     /locus_tag="VW077_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9454890.1"
                     /translation="MKMITVEHLQKNFVKTIKEPGLKGAFRSFFHPKRQVFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQENRQGYVKDIGVV
                     FGQRTQLWWDLALQETYSVLKEIYDVPDAVFQKRMDFLNEVLDLKEFIQDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLG
                     DIEQLCDRIFMIDKGREIFDGTVNQLKKTFGKMKTLSFELHPGQDYIVSHFEGLSDIY
                     VTRQELSLDIQYDSSQYQTADIIQQTLSDFTIRDLKMTDANIEDIIRRFYRKEL"
BASE COUNT         7416 a         5189 c         3981 g         7529 t
ORIGIN      
        1 gactcgccga gaactttgac atcagatata ctataacaaa ttgaaataag aatcaaacaa
       61 tttaattttg aaattcaaat caatttataa caatatttta gaagatatgc tgtgctattc
      121 cagattcagt ttgctataca tgcctgtcgt accacaaaat cccgtgtctg atgaggcacg
      181 ggatttttta gcttctttac tgagatggat tttccttttt agttcgtgcc aatctcaagg
      241 cacgatttgg attgagacga ttaaatagtt cttccaattt cttttcattc caaacgtctg
      301 cggcggaaac aaaattacca tccgcatctc ggaaagatat cggtttatct cccataccag
      361 tctcctagtc tacaaattca aactcaaatt taccaatgcg gaccaaatcc ccatctttag
      421 ctccacgcgc acgaagggct tcatcaaccc ccataccacg aagctgacgg gcaaatttca
      481 tgacagattc atcacgatca aagttggtca tattaaagag tttcatgagt ttttcaccag
      541 aaagtaccca tgtcgcatcg tcatcacgac taatttcaaa ggctttttct tcttcgtcaa
      601 atccatagta agcttcttct tccatatcgg actcgtcgta gagcaaaaat tctggtgtct
      661 tgtctaacaa ttcagctgta gcatctaaaa gtgttaccag accttgcttg gtcaatccag
      721 aaattgggaa gatagctggt aactcttcaa attcatcata attttcagcc aattttttct
      781 taaattcttc aagattttcc tgactctcag gcatatccat cttattagct acaataatct
      841 gtggacgctc catgaggcga agattgtaag actccagctc tttattgata gctaggtaat
      901 cctcatatgg atcacggcct tcgctagctg acatatcaat gatgtgaagg ataacacgtg
      961 tacgctcgat atgacggagg aactgagttc ccaaaccaac accttgacta gccccttcaa
     1021 tcaaacctgg caagtcggct actgcaaagg attcacctga ttgggtgcga accataccta
     1081 aatttggtac aatagtggta aagtggtagg caccaatttt aggcttagct gaggtaataa
     1141 cacttaaaag tgttgacttc cctacagatg ggaatcctac taaaccgaca tctgccaaga
     1201 tttttagttc caattgtaac tcacgttcct gacctggttc tccattttca gagatttccg
     1261 gtgcaggatt ttttggtgtc gcgaaacgaa tatttccacg tccaccacga ccaccgtggg
     1321 caacgataaa ttcttgccca tgttcaatca aatctgttaa aaccttgcca gtctccgcat
     1381 cacgaacagt cgtaccttgt ggtactagaa ctctaaggtc ctcagcacca cgaccatgca
     1441 tccctttggt catccctttt tcaccagaat cagccttgaa atgacgattg tagcggaaat
     1501 ccatcaaggt acgtagtcct tcgtctacaa cgaagaccac attgcctcca cgaccaccat
     1561 caccacccca agggcctcca ttagggacat atttttcacg acgaaaggca accataccat
     1621 cgccaccatt accagccttg accttaatct tagctgtatc taaaaacata ctcattattt
     1681 cttctcactt taaaaaaggg ctgggaaatc ccagtcacta aattttcttg aatctatttt
     1741 atagattact gagggcacca attgcagttg caaaaattcc caataaactt gctactagca
     1801 tgataatcac gataaacaag gttattttct caaacatagt ttttttacga tttccattat
     1861 ctccaaatgc catttttctc tccttcgtta cattctattt tctattatct tagcatgaat
     1921 tgagctagtt ttcaatagtc agttgctact ggtgcgataa tccataaaat aatgtaagct
     1981 acaattccag ctccgtaaca gcaagtaaga acaccccaaa tgactcgaac gatagtcgga
     2041 tccatatcca gataataggc caccccagca caaacacccg caatcttttt atcacgaccg
     2101 cttctcatta attgtttttt tcatagctgt tcctctttct attcttcatg gtctttccaa
     2161 tagtctctca tactgatcaa ctgtgttttt gaagccttca acatgcgtct agaacctttt
     2221 acaggtacag caaccacctg ttgcggaaga tacaaaactg tcttaaagat acgctgactg
     2281 accgtatcat ctttagccaa atctttctgg aagttatttg aaataatagc atccagataa
     2341 aactcatgca ctcgtttccc aaagttctca gctgaaatct catacaattt ctctgataag
     2401 gtatgctcgt tcatgtctgg tgttgcaatc agggcttcca aaatagcacc agccaaatca
     2461 tgttctccat agtacaaggt tccaaacatt ttatcactga tgaggttgtt caaataagga
     2521 tttccgtgag caatgacagg tgttccactg gctaagcttt ccaagtaggt caaaccttgc
     2581 gtttcgcttg tcgatgccga aatgaagaaa tccgccgctt tatagtaaag agccgtctca
     2641 ctaggagcaa tcatccctgt aaagatgact gagtcttgaa tctctaggtt ctgagcttgc
     2701 tctttgaggt cattcagata agggccatcc ccagctacta ccagtttaac cttgtcttcc
     2761 tctttcagaa catcagcaaa ggctgctaaa actgcttgaa tatttttttc ataagagatt
     2821 ctcgaaagac taagcaacgt cttttcacca tcttgaatcc ctagtttact acgcagttct
     2881 ttcaaatttt cctgcttgat ttccggacgc tcaaacttgg ctaattcaat cccagtagga
     2941 atgacccgtt tttcaacctt gaccttataa tcagatagca agtcacggac aatctcacta
     3001 ggacaaataa ccccatccac atcatgcagg aaacctctaa ccagatactt gaccatactc
     3061 ggccggatca acatcccctt agcaatataa tggacatagt cttcatactg ggtgtgatag
     3121 gtatggatga ctggaatttt caattcacgc gcaatccaaa tccccaacag gccaagagaa
     3181 aattctgtct gagtatggat aatatctagc tgatactgtt tagcaatttc aagtgccttg
     3241 ctaaaacctc ggtaggcaaa gcgacgatcc ttaaaagcaa agaaaggaac acttggaatg
     3301 cggataattt gccaatcttc gtagcgattg acatccttat ctgtcgtcgt aaagataaaa
     3361 acagcatgtc cctgcttttc aagttctgtt ttcaaggttc gaatactggt cgcaacacca
     3421 gaaacctgag gaaaataggt atctgtaaat aaaccaattc gcatagatta cctcactttt
     3481 tactttctcc ctaaagcggc ttgtttctca taaaagtcca accagatttg taacagattc
     3541 tcttcagaat actctctgga aatattctta gccttttctt tgagatcttt taaggcagca
     3601 ggatttgctt gatattccaa aatagcctct ttcatctctt ctctacccgc tgtcgcccga
     3661 taatttccct ccaaaatcac cttatagaga tctaaatcac gcaacataat aggagcctca
     3721 caactcgcag cttctaaaat agtcatagga aagagctcat tgtaactagg caacaagaaa
     3781 agatccgcta gagcatacaa ttcgcgcatc cgctctggcg atacaatgcc tggaaaaatc
     3841 aaatttttag ggggattttc cataattttc ttatagtgtt cataaccatc tgtcatacca
     3901 ccaaaagaga agccaccagc ccagataaag gtaatctgag gcaattcctc agccagacgg
     3961 ataaagtcat caatcccttt acgtttctga acttgcccag cacctactac gataaactga
     4021 ttgtcactaa gaccaagatc tgtgcgcagt ctgactacct cttcttgtgg tagaggatgc
     4081 catttttcct tgttgacaaa gttaggaata taggtcactt tttcacgtgg aataccagct
     4141 gctaccaaat cctcaataaa cataggattg accacaacca agtgctccat ccggttgtaa
     4201 aaagaaaata catagcgttt cacaattccc tttaagaaaa atggaatttt caaacttccc
     4261 tcaagtgtag ctggcaagaa atgcacatag ccaatctttc tccctgagcg tttcttttgg
     4321 aaggttgata aataataggg aaaatcaatc gtatgaaagt gagtcacatc tgcctcgatt
     4381 ggaagatttt ctgtaacaat caattggtcc ttggcagcac ggtgaagaag acgaactaat
     4441 tcacggtaag cacctgaaac tccctgtcct gctactttct cacttgaact caacatattg
     4501 atgcgtaatt tctttttctc cataactact attatatcat tttctttgaa taaaatcagc
     4561 aaaagaaagg agagactaga ggaaaagagg ggaaatttcc tcgcttttcc aacagtctct
     4621 cacatgcttt tatatgttta cttaatgcct agggcaatgc gggcatagcg actcattttt
     4681 tcaactgtcc aggctggata ccagactaat ttaacctcag tatccgttac ttctggaacc
     4741 tctatcatag catcataaat ctggtctgtc aaaaggtctg ctaagggaca acccatagtt
     4801 gtcaaagtca tgtcaatctc tgtttgccct gtgtcaccgt caaaacgaat ctcatagatc
     4861 aaaccaagat tgacaatatc gattcccaac tcagggtcga tgacttcttc caaggctgtt
     4921 aaaatccgtg ttttgatgtt ttcaatttgc tcttctgtat aagccatatt ttcctcactc
     4981 ttagtcttca ataaaatcac gaagcggttt gctacgactt ggttggcgta gttttctcaa
     5041 agcctttgct tcaatctgac ggatacgctc acgagttacg ttaaagactt tccccacatc
     5101 ttcaagtgtg cgcatttttc catcatctag tccaaaacgt agacgcagaa cattttcttc
     5161 acggtctgta agagtatcta agatttcatc caattgctca cgcaagacga tacgagtcgt
     5221 ataatccact ggattttcaa tcacttcatc ttcgataaag tctccaaggt ggctatcgtc
     5281 ctcttcaccg ataggagttt caagagatac tggttcttgg gcaatcttca agatttcacg
     5341 aaccttatca ggtgtcatat ccattcgttc agcaatctgt tctggtgtcg gatcttgccc
     5401 caattcttga aggagattcc gctgttcacg aaccaattta ttgatagttt caaccatgtg
     5461 aactgggata cggatggtac gagcttggtc cgcaatagca cgagtgatag cctgacgaat
     5521 ccaccaagtt gcataagttg aaaacttgaa ccctttagaa tagtcaaact tgtcaaccgc
     5581 cttcatcaag cccatatttc cttcttgaat caagtcaagg aactgcatac cacgaccgac
     5641 atagcgtttg gcaatggaaa caaccaaacg aagattggct tccgcaagac gttgtttggc
     5701 ttcgatatca ccagcttcaa cagccagtgc caactctttc tcctcttcat tggtcaagag
     5761 aggaacgacc cctatttctt tcaagtacat acggacaggg tcattgacct tagcagaagt
     5821 tgacccaatc aagtcctcat cgctgagttc tggttcttct tcattgctga gaacacgcgc
     5881 acttggattt ccttcgttat ctgtgataga aatgcctgca tcctgaatcc gttgcaagag
     5941 atcttcaatc ccatcagcgt ccaaggtaaa aggaataacc agacttgcat tgatttcatc
     6001 atctgttgct gtcccttttt gcttatgatt acggataaat tctgctacct gtacgtcaaa
     6061 tgttgttact tctttttgtt ttgttgccat tattactcca ttcttctctt ttgggaaatt
     6121 aaacgttcca attcttctag ggctgtatct gtatctccta catggctagc ttcctgcacc
     6181 ttctttttga ttctcatatt gtcctgattc aagagagcct tgtttcgagt catctctact
     6241 tcactaagtt cctgcggcga tatctcagca ggcaaatcct gagctaaaac ttggtaccaa
     6301 gctctttcaa cttcctctgt ctgctctgct aaaacttctg gaggaagatt tccatactgg
     6361 ccaagcaagt catataagac ctgaaattca ggtgtagcaa atgcaaagtc ttctcgcaaa
     6421 cggtaatcgt tcaaaacaag aggggattcc atcatccgat agagtagatg ggcttctgcc
     6481 ctcataatag ccgataactg cttggtgaca ggcatggtga ttggcgtcgg tctggaaatt
     6541 ctttccatgc gattctgcct ttgcacctga cgactctcat taacaatctg ctcaatctgg
     6601 gtataatcaa aggacgccag actgtcagct aaaatatgaa tatagctgtt ttgagcagcg
     6661 atggactttt cttgaacaat caagggagct attttttcaa taaactcaat ctgagcctgc
     6721 agattttcac tgttttcagg tttgtactga tgaatgtaga actcaatcgg actaatacga
     6781 gtttttgtta atagataggc caagtcttct ggaccatttt tttgtagata ctcatcagga
     6841 tccaagttat caggcatgct aacgatttgc acaggcatat caccaatttc atccaatgct
     6901 ttcaatgtcg cggcttgccc agccttatct ccatcgtaaa caagaaccaa tttcttggtt
     6961 aaccttttca gatgctcaac atgctctcga ctcaaggccg ttcccatcga cgccacagca
     7021 ttttcgattc cagcccgata ggctgcaata acatccatga atccttccat caggtaaatc
     7081 tcactagctt ttccagaaga tctttttgcc ctatccatat gatataattc gtaacttttg
     7141 ttaaaaattg cagtcgatcg gctgttttta tacttagaag tttgtgaatc cgttttttgc
     7201 cagatacgac ctgagaaggc aatgaccttt ccttggtcat ttgtcagggg aaacataatg
     7261 cgattgtgaa aggtgtctac aaattgattg gcatccgaga gataaaacag gcctgaatcc
     7321 agtaaatcct cttcacgata ctgatcagac aaacgttgat agagatagtt tcgttctgga
     7381 ggtgctaaac caatccaaaa atgtttaagc acttcatctg tcaaaccccg ctgataaagg
     7441 taatttctgg cctcttcgcc catagtcgtt gtcatgagaa tagcatggta aaatttggct
     7501 gcatcttcgt gcatatcata aagatcttgg tgaggtgagg ctgacttttg ctcactataa
     7561 agcggttttt caacctcaat tccaacacgc tgacctaaga tttggactgc ctctataaag
     7621 ggaacccctt ggtactcctc tatgaactta aagacatcac ctgagcgacc acaaccgaaa
     7681 cagtgataaa actgcttatc ctctacaacg ttgaaagaag gtgttttttc accatgaaaa
     7741 ggacagagcc ctagatagtt ccgtcctgcc ttttgtaaag aaatcacatc tcctatgact
     7801 tccacaatgt tggcattgtt tttgatttct tcaatgactt gtttgtcaac catacacaat
     7861 acctccatgt tatcatagtt tactttatat agtatacttt atttcagaaa aaaagtaaac
     7921 catttcactc attttcctta ctttattcaa agagttgata ataatcagag attttcattt
     7981 ttgctttttc ttcttggttt aaatcttgga taatccgtcc ttctttcatg acaatcaagc
     8041 gattgccata tttgagagca tcttccatat gatgggtaat cataagggct gtcaattgat
     8101 ctttcttaac aaattcatct gtcaattcca tcaaagcaac actagtcttt ggatccaggg
     8161 cagcagtatg ctcgtctaac aagagtaatt caggtcgctt caaggttgcc atcaagagac
     8221 tcaaagcctg tctttgtcca ccagataaga actcaatcgg tgtattcaag tgtttctcaa
     8281 gaccgtttcc cactttttca atggtcgcct gaaacttatc cttatagcta gccagacgtc
     8341 tcggaaatag tccacgcttt tcaccacgaa acttggcaat caggagattc tctgccactg
     8401 tcatacgagg agctgtcccc atctttggat cttggaagac tcgagacaga tacttagcgc
     8461 gcttctctgg tgaaaacttg gtaacatcct cacctaaaat acggatagtt ccactggtta
     8521 gtgatatggt tcctgcaata gtgttaaaga gggttgattt accagcacca tttccaccca
     8581 aaatcgtgat aaagtcctgt tcaaaaattt ctaaggaaac atcatttaaa ataatctttt
     8641 cttcatcaaa gccattttta acgattttgg ttgcattttt taattctaca attgctgtca
     8701 tttgcttaac ttggctcctt tcaagattgt ttgcttaaat gttggaatca tgaggcagac
     8761 tgctaaaatc aaggcactgt ataaacgaag gtaacttgta ttaaagccaa gtgcgataac
     8821 tgcccacact aaaaattgat aagcgataga acctacaacg atagtaacca aacgctctgc
     8881 caagctcaaa ctcttgaaaa taacttctcc aataatcaaa cttgcaagcc ccacaacgat
     8941 aaccccgatc cctcgagaca catcggcata accttcttgc tgagcaatga gggcacctgc
     9001 aagggcaatc acaccatttg ataagaccaa gcccatgagc tccatgcgtc cagtatgaat
     9061 cccgaaactt ctagccatat caggattatc ccctgtagca atataggctt gtccgagttt
     9121 agtgtccaag aaaaagagca tgagagcaat aacaatactc acaaagatga gacctgtcaa
     9181 gagttgattc aaatccgaat caaaaggcaa aacatcctga atttgcttgg ttccaagcag
     9241 gcctaaattc gcacgtccca taatcaagag catgattgag tgacaagaag tcatcaccaa
     9301 aatccctgag agcaaggttg ggatcttccc ttttgtataa agaaggcctg ctgccattcc
     9361 agccaaacaa cctgctccta cagcaacaag tgtcgctaaa aatgggttca cgcctttggt
     9421 tatcaaagtg acagcaacag ctcccccaag agggaaggaa ccttctgtcg tcatatctgg
     9481 aaagtttaaa atcctaaatg tcataaagat tcccagacct agaatagccc agacaaatcc
     9541 ttgagaaata atggaaacaa tcatatttta tttaatcctt tctatattca tctttttaaa
     9601 aaatgggaag agtctcctcc tccctacctt atttattcga tgacttgtcc tgcttctttg
     9661 agaacagact caggaatagt aatacctagt tcttgtgcta tttttttatt gatgactgac
     9721 ttaccagttg aaaagacatt gactggggta tcggctggtt ttgcaccttt caagacttgc
     9781 acaatcattt tacctgttgc cacaccaagg tcatgttggt caattacaac tgatgccaaa
     9841 ccacctactt ctaccatagc tgtcgcactg ggataaattg gtttcttaga actttgattg
     9901 ctagagacaa ccgttggaaa tcctgatgca atggtgttat caattggaac ccaaatagca
     9961 tctaccttgc tagtcataac agtgacagtt gaggcaattt catttgttga aggaactgca
    10021 aatgtttcca ctgtcagacc tgctttttca gcataagcct taaattcttc gacctgtgtt
    10081 tttgaattgt cttcgctact tgagtaaaga gctccgattg ttttcacatt cggtgtcaga
    10141 gccttgatga gttcaacttg ttgttgagct ggattgtggt cagatacccc tgtaacgttg
    10201 ccacctggtt ttttcaaatc tttaaccaag ttagcaccaa ttgggtctgt aatagcggcc
    10261 atgataaccg gtaggtcttt tgttgcacta gccaaccctt gggctgctgg tgttgcgata
    10321 ccaaccacaa ggtcattccc atttgcaacc aattgtttac tcattgtcgc aaccttactt
    10381 tggtcacctt ctgagttcat aaaatcaatt ttaacttgat catctttata tccttcttct
    10441 gcaagtccat cttggatccc tttataaatc aaatcaaggg atggatggct cacaaattga
    10501 agcacaccaa tcttggttat tttcttctca tccttattat tctgaacttc tgatttattc
    10561 attgaagaat aaatcaagct tcctgctact aagactgcta atgcagcaat aattccaatt
    10621 aaacgtttat ttttcattct gtttctcctt tttatttcct ttaacatatt tcacttatct
    10681 aaacaagcga cgccatcgtt ttctttccat actaacctcc atcaaaaaaa tcctcacaca
    10741 aaaaacttgt gtgaggacgt cgatgcgcgg taccacctca attataggga atttccctat
    10801 cgctcagtct cgcaataacg agatgcactg taaggtgtgc ttaccgaatt tttataattt
    10861 caaattctaa atagcattca gcccaatttt catcattgcc acttatctgt tttcagctac
    10921 cacagactct ctaaaaagtt tgtatgatta cttttctgaa tggttaaatt atatcatttt
    10981 taaactactt gtcaatagtc tattagaatt tttttgatat tcacttttag cattcactct
    11041 tgaaaattat caacctgaat tacgcaatcc tgtcgcaatt ccgttgatgg tgatatgaat
    11101 caatcgttct tgatcactgg acaattctcc acgacgttgg cgtttaatca actctaactg
    11161 aatatagttg agaatattaa agtaaggcat acggtaatcc agactagctt ttagatatgg
    11221 attgtcagct aagagttcgt catgtccttc aatagccaag ataacgttct tagtaacttg
    11281 ccattcattt aaaatagtct catagatggc cttaacttgc tcgtcttcac aaagtttagc
    11341 atattcaaaa gcaatattca tatttgattt tgacaaaacc atatcaacat ttgaaagaag
    11401 cgattggaag aaaggccaat tttggtacat atctcgtaag atagcaatat tctctggatt
    11461 tttattgata aattccttga agcttgaacc aaccccgtac catccaggga acataacacg
    11521 actctgtgac catgagaata cccaagggat ggcacgcaaa ccaccgattt cagtaatagt
    11581 cttacgagcg gctggacgag aaccaatatt aaaacttgaa atagccttga ttggacttga
    11641 ctcgaagaaa taatcataga aatgctcatt accaaagacc aaatcacggt agatatcgta
    11701 actacggtcc actacttgat ccataatggc ttcataacga tttggggtat tggtatcgct
    11761 cttcttctga gtaatcatac ggttaatagc tgccgatact agcatttcaa ggttatagta
    11821 agcggcgtct ttgttaccgt atttattccc aattacttca ccctgctccg tcaagcggat
    11881 acgatccttg atagacttga gcggttgaga tgtaatggct tcataggttg gcccaccacc
    11941 acgaccgaca gtaccaccac gaccatggaa gaaggtaacc ttaacgccaa attcatctcc
    12001 aatagcagtc aattgttgtt gagccttgta gagggtccaa catgatgaca agtaaccgcc
    12061 atctttatta ctgtcagagt agccaagcat gatttcttgg tagttatttc gtgagtcaat
    12121 ccattttttg gcaaggctaa gagaaagata ttttctcatt gtttcctctg aatgatccaa
    12181 gtcttcaatt gtttcaaaaa ggggaacaat ctgaacacgc gccctttccg tatccaccag
    12241 tcctacttct tttaacagaa tagctaattc tagcatatca gaaaggctgg ttgcatgtga
    12301 aatgatggtc tgacggatga catcatctcc caacttatct ttcaaaacac gagccgtctt
    12361 aaaaatagct aattcttttg ctaataattc tgatttttct gcgtgagtcg cagaaagaat
    12421 tcggggatct tcttctaatt ctttcaagag aaggtcacac ttttcttctt cgctcaactc
    12481 gctataacga gaatgaattc ctgctgattt caagagttct gccacacagg cttcatagac
    12541 gctagagtct tgtcgcatat caattgatgc taagtaaaaa ccaaatatct ctactgcctg
    12601 caataattcc acaaaatcac ctgaaatcaa ggactcgccc ttattttcta gtagagaatc
    12661 tcgaatggca atcaaatcct tgtagaaatc attggcggtt tcataacgag tcccaacttc
    12721 ttcatcctca atcagatagg ttttagttgc ctgaattttt gattgaatat caaacaaggc
    12781 acgacggtaa agctcttttt cgcggtaaat cgagttatcc ttggattgac gagccatttc
    12841 tctgacttgc ttgctgacgt tgacaatgct agttgaaaga gaaaattcac gataaagttg
    12901 gtaaatcttt ttatcatagt agttcatgat gacttcacac tgagtgagtg cagactgctt
    12961 caaggtcttt gctgtaacaa atggatttcc atcacggtca ccacctatcc acatacccat
    13021 ggtgattggt ttagcctgtt ttaaattcag accatgcgct tgcgctaagc gcttatactc
    13081 cgtcgtcaaa tgaggtacag ctttcaaaaa ggagctgtta taatattcca tagcattcgt
    13141 gatttcgtta gtcactttta attttttctc acgaatcatg tctgtctgca tgataatttc
    13201 gatgtaacga cgcaaatcat tgtaccattt atctttattg atcaacccca acttaacatc
    13261 acggtattta cgcaaaagac tatgaatatg atttgttaaa tccaacatac ttttgcgttg
    13321 cacttgtgtt ggatgggctg tcaaaacagg gacaacattc aagtgttcta ggatctcaac
    13381 ggcattttcc ttttctgcta ccaatttaat cgttgtagat aatttaccta aatagtcctg
    13441 atcaatatta ttttgatgat tgatttcata agctaaatcc acatcctctg aaatattaat
    13501 caaaagaggc aagatagaga aatagcgtga aatatagacc atttcatcat ttgataagct
    13561 agtcactaga cggtttagac cttgataatc ttcctgcgtt gataattctt tcaactgtat
    13621 tattttttca aaggtctctg gggcaagcat atttttagta atatcttcca gtaattctgt
    13681 tagaatcaag acttcttctt gcacaacact tttattacta taattttcta atttttgaag
    13741 agacataaac tatcctttct ttatcctact tcaacagaag gttcattggt tgattttcca
    13801 attctcggta caatttagcg cgtttttcac tggcatcaat atttaagaca aaggctactg
    13861 ccactgataa gactagaaga ctatttccac cctgggataa gaaggggaaa gtcactcctg
    13921 tagatggaat caagcccgaa atccctccga tattgacaaa tacctgaacc aacatcatcc
    13981 ctccgacacc gagtgcaacc atggcattga aaggattctc cgctcggata ccgaccaaga
    14041 taatccgcaa aatcatgaaa aacaagagag ctaaaataag actggcacca acaaagccaa
    14101 attcttcaat cacgatagaa aagacaaagt ctgtatgagc ttctggcaaa taacctcgtt
    14161 tttcaatcga gtttccaaga cctagaccaa accaaccgcc attgaccatg gcaaaataag
    14221 aattagctaa ctggtgacct gcatcagcac gatcggcaaa aggattaaaa aaggcactaa
    14281 agcgcttggc tacatagccg aatactggaa tttttgaaaa ggtctcaaca ccgattaggc
    14341 tgatagtggt caagacaaag acagaagcgg cagatacgag cgccagaatg gttgaaaacc
    14401 agcgataagc gattccacta actgtataca taatcaagga aaccaagact aaaatagtcg
    14461 catttcctaa atcagggaaa attcccaaac ttccaatcag aactaggaga acgaatcgcc
    14521 aatcattaaa agcacgggga agccattgat tttgagtcaa aacttgaaaa tcataagtag
    14581 ctatttcttc ttgctgtttg gagaatcggt gagctaaata ccaaataata atgattttta
    14641 agtactcagc tggctgaata gttactcctg caaccgaaat ccaaccgtat gccccgttta
    14701 cggaaagacc aataaaacga gccaagaaca atagaagcat ttctattaat ataactaaaa
    14761 tgattagtcg ctcatttctc aaaaaatcta gtctcaattt ataaattaag gcaatcagta
    14821 tcaaactaac aatccaaaag attccttggt ttcgaaccaa ctgcaaggca ctcttgcctt
    14881 cttcaattaa aatagcactg gtggtcgaat agaccacaat caagcccaaa atagataaaa
    14941 gcaagtaggg aatcaagatg gaataattta ataagtgcct cttactaatc ttcatagcat
    15001 caccactcta ctaggataca gacaaatctg ttccaaattc cgttttattt tctagttttg
    15061 attgctatta taccattttt tcagaaagaa aaaaactctt accttataat ccttcgaatt
    15121 ttactgtttt tcataattag agcagaattt gaaatcgtaa aaccattttg aacaagagtt
    15181 acttcaaccc attgactccg acggattaag ttgctttcgt gaatgccaaa atcagccgaa
    15241 atttgttcat aagtgcgcta ttctcgcatg tattgaatag ttaccataag gagatcgtct
    15301 aggcttaatt tggtttttcg tccacctttt gcgcgtttac gttgataagc tgtttttaac
    15361 acagctaaca tctcttcaaa agtcgtgcgc tgaacaccta caaggatctt gaaacggcta
    15421 tcgctcaatt gtttacttgc ttcatcatcc atagttttat tgtattgtat ttttgtttcg
    15481 caggaagtct attattcctt tcgttccaag gtttttacgc ttttagcaac aagcttacac
    15541 actgagcgtt tcggacttac catatcattt cgttggagat tttatcatca atttttagat
    15601 taaaaaagat ttcccaatct tcacaaactt ctgtagcagg aattgtatga atctttctaa
    15661 gtatcttttc tgcttctaca ccatatgtgt attgttggtt ttatgaataa gttaaaatgc
    15721 tatctattgc atctcttccg tctatccatt cgtgtaaaga atgtacttcg tcatcgcaga
    15781 gttcaatgct aattggttta cacatcggaa ctccaagaga agctactttc tccatcgtat
    15841 caaattcaat ttttttataa tctaacttcg acttatcaga aacacgcaag aaatatgttt
    15901 actgattttg aactgtcaca caatattttt tatcatctga ccagccttta tttatggcta
    15961 tcttactact aaaatccatc aagcacactc cgattcactt tacaacactt tttgcagttt
    16021 tcactagtaa cctatccagt tcgtctacac ttttatttcc tagatccatt tttacattat
    16081 gaaagttagt caattttctc tatgatgttt ccaagtttat ccaatacaag acaagaatga
    16141 tcatttagtg gaattatggg aacattaacg agttcttcag ctctatcctt atttgcttta
    16201 tcattccagg aatcataatg gacactaatt aaaaactgac taaaaagtcc taatccattt
    16261 tttatcttta tctgataatc tgaattaaca ttaggtgaga catagacttt ttctcctaat
    16321 agtagggctc ctgcagaaaa tcctataatt tttgcctctt tattaagcat atgatcgata
    16381 taacttttga actcctgatt aacataagta gcaatatatt tttccgtatt tccaccaccg
    16441 atgataatta tgtcagcatc tagatagcta tcaagatcaa tctgctcagt gtccaggagc
    16501 aagtaatcaa tgtttagatt aggaaattga ctttgaaaaa cttccgtgta ctttttcatg
    16561 tacggttccc aattttctcg aaaaactgta aaaatgacaa ttctttcgat cttccttgat
    16621 gacacaattt tattaacaat cgtatagttc tttatcggcg gattcccacc catcaaaaaa
    16681 atttgttctt tcaagttatc acactttcta aagatacata aagtttaaac aagtttattc
    16741 acgtttcgaa attataattt ttttaaaaca tatcatttta ttgactggct aagatcgaac
    16801 cagcaacact acacactcca cgtgtgttgt ctgttccttg agaatacaat tttttatcct
    16861 cgtcactgtt ttgaggacac aatttatctg tttctagttt agcattttta cgtttacttt
    16921 taaattgttt accatccttg ttttgaattt cacctgtttt gaatattatg gttaacattg
    16981 caggatcaat ttctccgttg ctattaactc cacctacaat aattttctca acaatacttt
    17041 caaaaactgc acgatcaaac tccgtaagca ttttttgcga agatagcaag tgcttaaact
    17101 cagttagtct tttctttaca tcgatttctg attttaatgt tgtttcaata tttaccttct
    17161 cttctgctag aaactctttt tctgaggaaa tttcattgta cttttcattg tatatggaat
    17221 cgctaacttt tccttcgagc cttagattta caagattctc ctcttttttt agtaatattc
    17281 gaagtttgtt tgtaatcctt tttagttcct tgttaaggct attgtcattc aattcagatt
    17341 ctatcgtttc aagtaagtct gtcattaaat tttcatttga atgatagact tgtctgtaag
    17401 cttccaggaa agccccctca atggcgatct cttctaatcc tttactatgc ttacaaaatt
    17461 tctttccttt ctttatcgat gtaacacagt gccacacaac tttgcgataa tctgaacggc
    17521 agtgccaact tcttctagat aatattgaac cacaaaaacc acattccaac atgctactaa
    17581 aagcatacat ttttgaatag cgttcacgct tcccatttac attcgcagct gtcttcttat
    17641 ttcctgctcg acgcaatcta atctcctgag ctttttcaaa atcttcttta gatattattg
    17701 gttcatgatt atccttgata tagtacttat cttcttctcc aaaattactc aaccgtctct
    17761 tgcttattgg gtcaactgta aaagtttttc ccattagaat atcaccttta tatttttcat
    17821 tctttattat tcctagaact gttgtatcat tccagtgttc taaacctctt ggcgatttgt
    17881 aaccaagttc atcaagttcc cttgcaatta cttttcctcc aataccttct agataccttt
    17941 caaaaatata acgaacaatt ttggcttctt ttttattaat tgaaatctgc ttggtttcta
    18001 catcataatc ataccctaag cacccttgaa aaccaaccaa ttcaccacgt tgcatcttca
    18061 tttttaatcc ttttttcaca tgggcagaag tattctctac ttcttgctgt gcaacggagc
    18121 ttagaatagt tagaagcaat tctccatcca ttgttagggt atctatgttt tcttcctcaa
    18181 aatatactcc aatttgcata tcctttaaca ttctgacata cttcaaagta tctagggtat
    18241 tccttgcaaa tctagcaatt gcttttgtga caatataatc tatctctcca tttgcacaat
    18301 cattgattaa tcgttgaaaa tcttgtctct ttcctacttg tgttcctgaa ataccttcat
    18361 ctgcataaat atctgccagc tcccaatctg aacgctgaga aatatattct ttgtagtgtc
    18421 ttacttgaga atcataactg tttttctgat cctcactatc ggaactaact ctgcaataag
    18481 cagcaaccct ctttaagtgt ctttctacat tgtcgcctct tcgacgaact aaagaatcag
    18541 cttttattat ttcaacatta ttttttttca tactgaaatt cctccttttg attctatatt
    18601 accgcattca aatcaaaaag tcagctattt tgtgtactta atacgcagtt tgtttctaat
    18661 cttgtcatac tcatttttag ataacatatt tcttgagtat aaaaagttaa gcattgatag
    18721 ttgcatagta tactttatta ctgattgttc caaacttttt cctccttgta acatgtataa
    18781 tttcctccgt gggtatgact ggttaaatca ttcatgggca tttcacccgc tgtccttttt
    18841 cgctggcagc ctgaacgttc agaagtatca ttatccttat ttgtagtcat ggcagataga
    18901 gaaccactct attttatagc taataattat cgctcctatc acggcgtatc aagctaaagt
    18961 gctcgacaaa taagaaagtc ccacctcggt ttattcagtt ttcaaagggc ttaggattta
    19021 ttaacaacta aacttatcca tctctaaaat ataatctgta gttacctgat ataatttagc
    19081 taattgtttc aaaaatgatg taggaatatc ttgcttgcca ctttctattc tagaataagc
    19141 agactgtgaa caatgaagat actgtgcaat ttgacactgg gacaaatctt tgtcttctct
    19201 taaattcttt aatctgatat acattaataa tcctcttact aatattgtaa tttgaagatt
    19261 atcaaatttg gaattttatg caaaatatgc ataaaataat ttataagatt ttagaaatag
    19321 ttggtataac agtaagtaag ctataagtta ctaaaaaaag attttggaat aaactttttc
    19381 atagcgataa aagcaccgta caagctagga aaataataag taatcgccca atctgacctt
    19441 ctccagaact tgattattcg tcaatcacta cagttgacaa acatactaag attagtagtg
    19501 aggaaatctc caacgggaga gagtactcat tactttttct ttgtgttaaa atagtggtgt
    19561 cttgttaagt catagagctc tctatggtgc tagacgtcgc ttgacaaact ggcaagaaac
    19621 ctgttttagg aagaatttta tcaaagtatg tgggatacta gacgtcgagt attggaaata
    19681 aaatcactaa aacaaggagt cattcaagac aaaggggtaa acatgatgcg gacagttttc
    19741 agaatggatg tgagtaaggc aagctcagaa gtcgctattc ttgtgaatgg tgaaaaggtt
    19801 catggctata ccatgcccaa tgatgccatc ggatttagtc gtcttctaga ggatttgaaa
    19861 taggtaaata cacctgagat tatcttcgag gcacaggggt ctattctcgt cttcaggcct
    19921 ttctggagga caatagctat gcttatatac ggctaaatcc tttaaaacta agaagcaact
    19981 agacagtttg cgagtccgta aaaccaatca gattgacgct gaaaagctag cttcgtcaca
    20041 attcatcctt aaccgcaaac caacttatgt tcaagaagaa gtctatcaaa atttgcgtga
    20101 cctcagtcga ttctatcaga atctgacaga agacatcgtt aggactaaga atagcctgca
    20161 caaggtgctt caaattgcct ttccagaaat aaagaacctt ctctctactc ctacagggga
    20221 acaatattgg aacctcgtga ccatctttcc aaccaagcat tggaggctga aattatcagt
    20281 agttgagcta aaagaagcca tcgtaattca acttctaaac ggatttctga gaacagagtt
    20341 ggacgattgg ctgaaaagtt gagcgagctg gccgaacagt cctatcttgc catttcaaaa
    20401 acctctccca tgacagaaga tgttaagtat catgcacagg aactgttgag actgacaaac
    20461 cgcaaggatc aggacttagc acaaatgatt gaattggctg aaccattacc tgaatacgag
    20521 attctgctct ctattcctgg tatcgcagaa acaacagcta caagcattat tggcgaattg
    20581 gagacattcg tcgctttcag tctgccaacc aaatcaatgc ctttatcagt attgacctca
    20641 gacactatga atctggcaat ttcctagctc aagaacatat caccaagcgt ggaaatcctt
    20701 acgcgagaaa aattctctat aagtgcgtcc acaatattgc ttcagctagt cataccaatc
    20761 cttgtcatat cgcggacttt tatgagaaac gaaaaagaca atcgcagacg acttcaacca
    20821 agccatatac gattgtctct atacatcgcc tcatccggac aatgtattac ctcataacgc
    20881 ataacaaact ttacgactac actttaaccc aaagtcatta aagtcatcta tgtcctatca
    20941 ttgtaacacc ttatttttta ataccagata agtgtctttt aagtgtgccc aaatttcaaa
    21001 aaacagctcg caattatctt ctaaacattc ctttgatact cattcagaaa accttttgag
    21061 ctagaatatc gtatgtccct taacaaatgg tatgtataaa agagctaaaa aaccactcat
    21121 gatatgaacg agtggttatg actgaaatat tacatacctt tgtatcattt ttgcacaatg
    21181 gtcaaatttc ttgtttgaaa aactacagta agcctaatca aagctctcaa tgtatcatta
    21241 aaacttcctt ccaaagaata ggaagttcta tcaaaattct taattagatt ttacttcttc
    21301 taagaccctg cgctctcata tgcatccaaa cctttgttcc ataattttaa tgataaggta
    21361 aaaaatatta atgagataag gatcaatcca ccaatattga acaatccatc cttgtctttc
    21421 aagaaatagc tagctgggta ataggctgta aaagcaaaag gaatgataaa actaatcaac
    21481 caacgtaaaa acgaatggta aatcgcaatc gggtattttg caaaatcatt aaacatgtaa
    21541 aaaatataaa ttattgcccc agattgttta gtccaaaaag caatactagc tgttacaatt
    21601 tttaaagagg tataaattaa ggttgcaaag ggaatactga ttaaaaagag aaaaaccttt
    21661 gcccaagtcc aagtaagact agttattgtc attaacagca ataagacgcc tactaacaat
    21721 tctccaagag catcaatttg aaacgtttct actaaaatat gaaacaaagg gctaatgggt
    21781 ctagtcaaat acttatcaaa ttctccttta cgaattaaat gttgtcctaa agctcacaga
    21841 ttatcaaaaa agagatggtc aattccctta ggaatgagag aaaaaccata aataaaagca
    21901 acctgatgaa aactccagcc atcaagtaag ggaatatgtt gaaacaaaat atttagaaac
    21961 agcatgttta acccttgtgt caaaaagaca cccacaacac caactaaaaa atcagcctta
    22021 tactccataa tctgtttaag atattgtcgg ataaaaatca ggtgcatgcg ttgatatttc
    22081 ttcattctaa cctccttgaa tagttatata attctgtacg cacttccaaa tcagttggga
    22141 taaggcaatc ataataaaca gccagaataa ttgtagcgag agcgcctgaa tcatttgact
    22201 catgctatac ttttctataa taaccatcac cggggtatag attaaagaag aaaatggcaa
    22261 aaaagataat agttcggcaa taatttttgg aaaaaatgtc agaggaacca aacttccaga
    22321 taaaaaggca actaaagaat ttttcaaaag atttgatccc cataagtttt taaacacaaa
    22381 agctgaaaaa ccaaagcaaa tattgaaaaa aaaaattaat caaaaaagct aagattaagc
    22441 taagaaggta aactgtggtt attaaaacaa tttgtaagaa ggactcacct gataagagct
    22501 ttagacctgc aatcagaatt acaaatggta gaccaacact aacaaaaact agccatctag
    22561 acccaatttc tgtgaaaaga taagacattg caaaatgaac tggacgaagc agtcgcataa
    22621 taatagagcc atcctttact tcccatccaa tcataaaaga actatctgat tttgtcagta
    22681 gattcgtcac aaaactcatg atgatatata aggtcatatc agataaagta aaaccttgaa
    22741 tgagagactg gtgagatgaa tcaaacacag ctttccacaa gtaaaatgct acaaaggccc
    22801 ccatcacatc cccaatgcga taaagaaaaa aattgacacg atacgtgatt aattcttgga
    22861 ttccagcgct aacaaaaggc ttatagcgtt tccataattt agtcatccta aagctccttt
    22921 cgatagaagc gacgaataat atcttcaata ttcgcatccg tcatctttaa atcacggatc
    22981 gtaaaatcag acaaagtttg ttgaataatg tcagccgttt gatactgcga gctgtcgtac
    23041 tgaatatcaa gtgacaattc ctgtctcgtc acatagatat cagaaagtcc ttcaaaatga
    23101 gagacaatat aatcctgccc aggatgtagt tcaaaagaga gagtcttcat cttcccgaaa
    23161 gtctttttca gttgattcac agttccatca aagatttctc ggcccttatc aatcataaaa
    23221 atacgatcac ataactgttc aatatcacct aaatcgtggg tcgttaaaag tatggtcgtc
    23281 tcttcttctt gattaatctg tgtaatggca cgtcgaatat tatccttgac cgaaacatct
    23341 aaaccaatag taggctcatc taggaaaaga actttcggat tatgcaataa ggaggccgca
    23401 atatccgccc tcatacgttg acctagagat agagttctga caggatcttg aataaattcc
    23461 ttcaaatcta aaacttcatt tagaaaatcc atgcgttttt gaaagaccgc atcaggaaca
    23521 tcataaattt cctttaaaac cgagtaagtc tcctgcaaag ccaaatccca ccaaagttga
    23581 gttcgctgcc caaaaactac accaatatct ttaacatagc cttgacggtt ctcttgagga
    23641 atctttccat tgatacgaca aaaacctgat gttggtttca aaatccccgt caacatcttg
    23701 atagttgttg atttccccgc tccatttgcg ccaataaagc ctaaaatttg tcctttgggc
    23761 acctcaaatg ataaatcttt aacagcttca aagacttgtc tcttgggatg aaaaaaagag
    23821 cgaaaagctc cctttaagcc aggttccttt attgttttca caaaattttt ctgaagatgc
    23881 tctactgtta tcatttttat aaatcactcc tttcgtttca ctcaagttat aatatcaaca
    23941 aaaattgtag catactacag taaagttaag aaaatttacc atcattacaa accatatgaa
    24001 aaaagctact aaaactatgc catcttgtat tttatctgta agtaagctaa attactcaag
    24061 tacaaagaat tatgacgaag aacatataaa tttataattg gataaactat agatt
//
LOCUS       DAVKGU010000021        23044 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKGU010000021 DAVKGU010000000
VERSION     DAVKGU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.021"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            337..2490
                     /locus_tag="VW077_000843"
     CDS             337..2490
                     /locus_tag="VW077_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9454891.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2503..3852
                     /locus_tag="VW077_000844"
     CDS             2503..3852
                     /locus_tag="VW077_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9454892.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATIEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4022..4729
                     /locus_tag="VW077_000845"
     CDS             4022..4729
                     /locus_tag="VW077_000845"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9454893.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            4931..8656
                     /locus_tag="VW077_000846"
     CDS             4931..8656
                     /locus_tag="VW077_000846"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9454894.1"
                     /translation="MDKRIFVEKKADFQVKSESLVREFQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPTVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8749..10191
                     /locus_tag="VW077_000847"
     CDS             8749..10191
                     /locus_tag="VW077_000847"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9454895.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10228..11250
                     /locus_tag="VW077_000848"
     CDS             10228..11250
                     /locus_tag="VW077_000848"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9454896.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11247..11792
                     /gene="purN"
                     /locus_tag="VW077_000849"
     CDS             11247..11792
                     /gene="purN"
                     /locus_tag="VW077_000849"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9454897.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11876..12385
                     /locus_tag="VW077_000850"
     CDS             11876..12385
                     /locus_tag="VW077_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9454898.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12410..13957
                     /gene="purH"
                     /locus_tag="VW077_000851"
     CDS             12410..13957
                     /gene="purH"
                     /locus_tag="VW077_000851"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9454899.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFSDNVEEIAKAGIKAIIQPGGSVRDQESIEAANKYGLTMVFT
                     GVRHFRH"
     gene            14079..15341
                     /gene="purD"
                     /locus_tag="VW077_000852"
     CDS             14079..15341
                     /gene="purD"
                     /locus_tag="VW077_000852"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9454900.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVIYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTVATVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15744..16232
                     /gene="purE"
                     /locus_tag="VW077_000853"
     CDS             15744..16232
                     /gene="purE"
                     /locus_tag="VW077_000853"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9454901.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPIIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAAIFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16219..17310
                     /gene="purK"
                     /locus_tag="VW077_000854"
     CDS             16219..17310
                     /gene="purK"
                     /locus_tag="VW077_000854"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9454902.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLISNEPDSVDELGEGIDF"
     gene            17320..17547
                     /locus_tag="VW077_000855"
     CDS             17320..17547
                     /locus_tag="VW077_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9454903.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            17610..18908
                     /gene="purB"
                     /locus_tag="VW077_000856"
     CDS             17610..18908
                     /gene="purB"
                     /locus_tag="VW077_000856"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9454904.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(18963..22901)
                     /gene="strH"
                     /locus_tag="VW077_000857"
     CDS             complement(18963..22901)
                     /gene="strH"
                     /locus_tag="VW077_000857"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9454905.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWTDTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASDLGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVLGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
BASE COUNT         6240 a         4628 c         5526 g         6650 t
ORIGIN      
        1 atttcggttt tgttaacttg taattcttat attttctagg tagaacgttt gttctgccta
       61 tttttttatc caaaaagtgc agttgggagg gagataggct catttgggaa ggaagtccag
      121 tttttgttta gtgattgggg taagatagtt gttatcagat gagtttatac tcttcgaaaa
      181 tcaaattcaa accacgtcaa cgtcgccttg ccgtatatat gtgactgact tcgtcagtcc
      241 tatctacaac ctcaaaacag tgttttgagc agcctgcggc tagtttccta gtttgctctt
      301 tgattttcat tgagtattag ggaaaaggag atgaatatga aatttgggaa acgtcactat
      361 cgtccgcaag tggatcagat ggactgcggt gtagcttcat tagccatggt ttttggctac
      421 tatggtagtt attatttttt ggctcacttg cgagaattgg ctaagacgac catggatggg
      481 acgacggctt tgggcttggt caaggtggca gaggagattg gttttgagac gcgagccatt
      541 aaggcggata tgacgctttt tgacttgccg gatttgactt ttccttttgt tgcccatgtg
      601 cttaaggaag ggaaattgct ccactactat gtggtgactg ggcaggataa ggatagcatt
      661 catattgccg atccagatcc cggggtgaag ttgactaaac tgccacgtga gcgttttgag
      721 gaagaatgga caggagtgac tctttttatg gcacctagtc cagactataa gccttataag
      781 gaacaaaaaa atggtctgct ctcttttatc cctatattag tgaagcagcg tggcttgatt
      841 gccaatatcg ttttggcaac actcttggta accgggatta acattgtggg ttcttattat
      901 ctgcagtcta tcattgatac ctatgtgcca gatcagatgc gttcgacact agggattatt
      961 tctattgggc tagtcatcgt ctacatcctc cagcaaatct tgtcttacgc tcaggagtat
     1021 ctcttgcttg ttttggggca acgcttgtcg attgacgtga ttttgtccta tatcaagcat
     1081 gtttttcacc tccctatgtc cttctttgcg acacgcagga caggggagat cgtgtctcgt
     1141 tttacagatg ctaacagtat catcgatgcg ctggcttcga ccatcctttc gattttccta
     1201 gatgtgtcaa cggttgtcat tatttccctt gttctatttt cacaaaatac caatctcttt
     1261 ttcatgactt tattggcgct tcctatctac acagtgatta tctttgcctt tatgaagccg
     1321 tttgaaaaga tgaatcggga taccatggaa gccaatgcgg ttctgtcttc ttctatcatt
     1381 gaggacatca acggtattga gactatcaag tccttgacca gtgaaagtca gcgttaccaa
     1441 aaaattgaca aggaatttgt ggattatctg aagaaatcct ttacctatag tcgagcagag
     1501 agtcagcaaa aggctctgaa aaaggttgcc catctcttgc ttaatgtcgg cattctctgg
     1561 atgggggctg ttctggtcat ggatggcaag atgagtttgg ggcagttgat tacctataat
     1621 accttgctgg tttactttac caatcctttg gaaaatatca tcaatctgca aaccaagctt
     1681 cagacagcgc aggttgccaa taaccgtcta aatgaagtgt atctagtagc ttctgagttt
     1741 gaggagaaga aaacagttga ggatttgagc ttgatgaagg gagatatgac cttcaagcag
     1801 gttcattaca agtatggcta tggtcgagac gtcttgtcgg atatcaattt aaccgttccc
     1861 caagggtcta aggtggcttt tgtggggatt tcagggtcag gtaagacgac tttggccaag
     1921 atgatggtta atttttacga cccaagtcaa ggggagatta gtctgggtgg tgtcaatctc
     1981 aatcagattg ataaaaaagc cctgcgccag tacatcaact atctgcctca acagccctat
     2041 gtctttaacg gaacgatttt ggagaatctt cttttgggag ccaaggaggg gacgacacag
     2101 gaagatatct tacgggcggt cgaattggca gagattcgag aggatatcga gcgcatgcca
     2161 ctgaattatc agacagaatt gacttcggat ggggcaggga tttcaggtgg tcaacgtcag
     2221 agaatcgctt tggcgcgtgc tctcttgaca gatgcgccgg tcttgatttt ggatgaggcg
     2281 actagcagtt tggatatttt gacagagaag cggattgtcg ataatctcat ggctttggac
     2341 aagaccttga ttttcattgc tcaccgcttg actattgctg agcggacaga gaaggtggtt
     2401 gtcttggatc agggcaagat tgtcgaagaa ggaaagcatg ctgatttgct tgcacaggga
     2461 ggcttttacg cccatttggt caatagctag aaagaggaga ggatgaaacc agaattttta
     2521 gaaagtgcgg agttttataa tcgtcgttac cataattttt ccagtagtgt gattgtaccc
     2581 atggcccttc tgctcgtgtt tttacttggc tttgcaactg ttgcagagaa ggagatgagt
     2641 ttgtccacta gagctactat cgaacccagt cgtatccttg caaatatcca gtcaactagc
     2701 aacaatcgta ttcttgtcaa tcatttggaa gaaaataagc tggttaagaa gggggatctt
     2761 ttggttcaat accaagaagg ggcagagggt gtccaagcgg agtcctatgc cagtcagttg
     2821 gacatgctaa aggatcaaaa aaagcaatta gagtatctgc aaaagagcct gcaagaaggg
     2881 gagaaccact ttccagagga ggataagttt ggctaccaag ccacctttcg cgactacatc
     2941 agtcaagcag gcagtcttag ggctagtaca tcgcaacaaa atgagaccat cgcgtcccag
     3001 aatgcagcag ctagccaaac ccaagccgaa atcggcaacc tcatcagtca aacagaggct
     3061 aaaattcgcg attaccagac agctaagtca gctatggaaa caggtgcttc cttggccagt
     3121 cagaatctag cctactctct ttaccagtcc tacaagtctc agggcgagga aaatccccaa
     3181 actaaggttc aggcagttgc acaggttgaa gcacagattt ctcagttaga atctagtctt
     3241 gctacttacc gtgtccagta tgcaggttca ggtacccagc aagcctatgc gtcagggtta
     3301 agcagtcaat tggaatccct taaatcccaa catttggcaa aggttggtca ggaattgacc
     3361 cttctagccc agaaaatctt ggaggcagag tcaggtaaga aggtacaggg aaatctttta
     3421 gacaagggga aaattacggc gagtgaggat ggggtgcttc atcttaatcc tgagaccagt
     3481 gattctagca tggttgcaga aggtgcccta ctagcccaac tttatccatc tttggaaaga
     3541 gaagggaaag ccaaactcac agcctatcta agttcaaaag atgtagcaag aatcaaggtc
     3601 ggtgattctg ttcgctatac tacgactcat gatgccggga atcaactttt cctagattca
     3661 actattacaa gtattgatgc gacagctact aagactgaga aagggaattt ctttaaaata
     3721 gaggcggaga ctaatctaac ttcggagcag gctgaaaaac ttaggtacgg ggtggaaggc
     3781 cgcttgcaga tgattacggg caagaaaagt tatctacgtt attatttgga tcaatttttg
     3841 aacaaagagt aatgttcgtg tttttagagt taaataattt ttaaactgtg agaaagattc
     3901 ttcttgcagt tttttcttta caatttttga aaaacatcta ctatttattc ggttaaattc
     3961 ttgtgttttt tggttttttg tggtaaaatg tgctcaagta atacgaaagg cgaactttaa
     4021 aatgtcaaaa caattgatct attcgggaaa agctaaagat atctatacaa ctgaggatga
     4081 aaatcttatt atttcaactt acaaggacca ggcgactgct ttcaacggtg tcaagaagga
     4141 gcagattgca ggtaagggag tcttgaataa tcagatctca tcttttattt ttgagaaatt
     4201 aaatgcggct ggtgtggcga ctcactttgt ggagaaactt tcagacacgg aacaactcaa
     4261 taaaaaggtt aagattattc ctttggaagt cgtgctccgc aactatactg ctggttcttt
     4321 ttcaaaacgt tttggtgtgg atgagggaat cgccttggag actccgattg tcgaatttta
     4381 ctacaaaaat gatgatttgg atgatccatt tatcaatgat gagcatgtga aattcctaca
     4441 gattgcgggt gaccagcaga ttgcctactt gaaggaagaa acgcgtcgta tcaatgaact
     4501 attgaaagtc tggtttgctg agattgggct taaattgatt gactttaagc tagagttcgg
     4561 ttttgacaag gatggcaaga ttatcttggc agacgaattt tcaccagata actgccgctt
     4621 gtgggacgct gatggcaacc acatggataa ggatgttttc cgtagaggat tgggagaact
     4681 aaccgacgtt tatgagattg tttgggaaaa gttgcaggaa ttgaaataat ctgtttgcaa
     4741 cggaaaacct tcgtctctca actaaaagga ctcaggctga aaaggtcccc cagacctttt
     4801 cactctgtag agaactaggt gaactaacag atgtttacga aattgtctgg gaaaagttgc
     4861 agggtttaaa ataacaacct caaggctgtt tgggaatatt gcaagagctg aaataaagga
     4921 ataagaattg atggataaac gtatttttgt tgaaaaaaag gctgattttc aggtcaagtc
     4981 agagagtttg gttagagagt tccagcacaa cttgggactg tcaagcttga aaagtattcg
     5041 tattgtgcaa gtatatgatg tatttgactt ggctgaggac ttgtttgcac ctgcagagaa
     5101 gcacattttc tctgagcagg gaaccgacca tgttttagat gaagtatctg tgcaggcgga
     5161 tcttgctaac tatgctttct ttgccattga aagtctgcca gggcagtttg accagcgtgc
     5221 agcttcgtca caggaagcct tgcttttgtt gggaagttcg agtgacgtga cagtcaacac
     5281 agcccaactt tacttggtga ataaagatat tgatgcgact gagttggaag ctgtcaaaaa
     5341 ctacctgctc aatccagttg attctcgttt caaggatatc acgacaggga ttgccaagca
     5401 ggagttttca gagtcagaca agaccattcc aaaattgact ttctttgaaa gctatacagc
     5461 agaagacttt gctcgctaca aggccgagca agggatggcc atggaagtgg atgatttgct
     5521 ctttatccaa gactacttta agtcaatcgg gcgcgtgcca actgagactg aactcaaggt
     5581 tttggacact tactggtctg accactgccg tcatacgact tttgagacag agttgaaaca
     5641 catcgacttt tcagcttcta aatttcaaaa gcaattgcag tcaacctatg acaagtatat
     5701 tgccatgcgc gaggagttag gtcggtctga aaaaccacaa accttgatgg atatggcgac
     5761 tattttcggt cgttatgagc gtgctaacgg acgattggat gatatggaag tctctgacga
     5821 aatcaatgcc tgctcagttg aaattgaagt ggatgttgat ggtgtcaagg aaccttggct
     5881 cctcatgttt aaaaatgaaa cccacaacca tccaacagaa attgagccat ttggtggagc
     5941 ggctacctgt atcggtggag ctattcgtga tccgttgtca ggccgttcct atgtttacca
     6001 agccatgcgt atttcaggtg ctggtgatat tacagcaccg atttcggaaa ctcgtgctgg
     6061 gaaattgcca caacaagtca tttctaaaac agcagctcat ggttattctt catatggtaa
     6121 ccagattggg cttgccacaa cctacgttcg tgaatacttc cacccaggct ttgtagctaa
     6181 acgtatggaa cttggtgccg ttgttggtgc ggctcccaag ggcaatgttg tccgtgaaaa
     6241 acctgaagca ggtgatgtga tcatccttct cggaggcaaa acaggtcgtg atggtgtcgg
     6301 tggtgcgacg ggctcttcta aggttcaaac agttgagtct gtagagactg ctggtgctga
     6361 ggttcaaaaa ggaaatgcca tcgaagaacg caagattcag cgcctcttcc gtaatggcaa
     6421 tgtcactcgt ctgatcaaga agtccaatga ctttggggca ggcggcgtct gtgtggctat
     6481 cggtgaattg gcagacggtc ttgaaatcga cctcaacaag gtgcctctta aataccaggg
     6541 cttgaatggt acagaaattg ccatctctga atcacaagaa cggatggcgg tcgtggttcg
     6601 tcctgaagat gtggatacct tcgttgccga atgtaacaaa gaaaatattg atgctgttgt
     6661 ggtggcgaca gtaactgaaa aaccaaatct tgtcatgcac tggaatggtg agacaatcgt
     6721 tgacttggag cgtcgtttcc ttgacaccaa tggtgtgcgc gtggttgtcg atgccaaagt
     6781 tgtggacaag gatgtcaaac tcccagaaga gcgtcaaaca tctgctgaaa cactggaatc
     6841 agataccctt acggttctat ctgacctcaa ccatgcaagt caaaaaggat tacagactat
     6901 ctttgactgc tctgttggac gctcaacggt taatcaccca cttggtggtc gttaccaact
     6961 cacaccaact gaggcatctg tgcagaaatt gccagttcaa cacggtgtga ctcatactgc
     7021 gtcggtcatt gctcaaggtt tcaacccata tgtagctgaa tggtctccat accacggtgc
     7081 tgcttatgcg gttatcgaag caactgctcg tttggtggct gctggtgcca actggtctaa
     7141 ggctcgtttc tcttaccaag aatatttcga gcggatggac aagcaagcag agcgtttcgg
     7201 tcagccagta gctgctcttc taggttctat tgaagcacaa attcagcttg gcttgccatc
     7261 tatcggtggt aaggactcca tgtctggtac ctttgaagaa ttgaccgttc cgccaacctt
     7321 ggttgccttt ggggtgacga cggcagatag ccgtaaggtg ctctctccag aatttaaagc
     7381 tgttggggaa aatatctact acatcccagg tcaagccctc tctgcagaga ttgattttga
     7441 cttgattaag aaaaattttg ctcagtttga agcaagccaa gctgaccata aagtgacgtc
     7501 tgcatcagct gtcaaatacg gtggtgtagt tgaaagtttg gctcttgcta cctttggaaa
     7561 ctatattggt gcagaggtga ccttgcctga acttaaaaca gctttgacag ctcaattagg
     7621 cggctttgtc ttcacatctc ctgaagaaat tgctggagta gagaagattg gacaaacgaa
     7681 agcagacttt acactgactg tcaacggtgt gaagctagat ggacacaagc ttgacagtgc
     7741 atttcaaggg acattggaag aagtttaccc aacagaattt acccaagcga aagaactaga
     7801 agaagtacca actgtggcat cagatgttgt gattaaagcc aaagaaaagg ttgaaaaacc
     7861 tgtggtttac atcccagtct ttccaggaac caactcagaa tatgattcag ctaaggcctt
     7921 cgaaaaagaa ggtgcagagg tcaatttggt gccattcgtg accttgaatg aagaagctat
     7981 tgtcaagtca gttgaaacta tggttgacaa tatcgacaag actaatattc tcttctttgc
     8041 cggtggattc tcggctgcgg atgaaccaga tggttcagct aagtttatcg tcaatatcct
     8101 gcttaatgaa aaagtgcgtg tggctattga tagctttatc gcccgtggtg gcttgattat
     8161 cggtatttgt aatggattcc aagccttagt caaatcgggt ctcctaccat acggaaactt
     8221 tgaagatgct aacagtacta gtccaaccct cttctacaat gatgccaacc aacacgtggc
     8281 caagatggtg gaaactcgca ttgccaatac caactcacca tggttggctg gtgtgcaagt
     8341 gggcgatatc cacgctattc ctgtttcgca cggtgaaggg aagtttgtcg tgacggctga
     8401 ggaatttgca gagctccgtg acaatggaca aattttcagc caatacgttg actttaacgg
     8461 taaaccaagt atggattcta agtacaatcc gaatggttct gtccatgcca tcgaaggaat
     8521 taccagcaag aatggtcaaa tcatcggtaa gatgggccac tcagaacgtt atgaggatgg
     8581 tcttttccaa aatatcccag gcaataaaga ccaacacctg ttcgcatcag cggttaaaca
     8641 tttcactgga aaataagact tacagatttt ctaatagata gtatcagtaa tgtaaaagtc
     8701 atgtaaatct agctcttgat gattacaaat gaaaattagg tataaaaaat gacatacgaa
     8761 gtaaaatctc ttaatgaaga atgtggtgtt ttcggtattt ggggacatcc agatgctgct
     8821 aagttgacct attttggact ccacagtctt caacaccgtg gtcaggaggg ggcaggaatc
     8881 ctctccaatg atcaaggaca actgaagcgc catcgtgaca tggggctttt atcagaagtt
     8941 ttcagaaatc cagccaattt ggataaattg acaggagctg gtgcgattgg gcatgtgcgt
     9001 tatgcgactg ctggcgaagc ttctgttgat aacatccagc ccttcctctt ccgttttcac
     9061 gatatgcagt ttggcttggc tcataatgga aatctgacca atgcagcctc tctcaagaaa
     9121 gaactggaac aaagaggagc aattttcagc gcgacttcgg actctgaaat cttggctcac
     9181 ctcattcgtc gtagtcataa tcctagcctg atgggcaaaa tcaaggaagc gctcagcctt
     9241 gtcaaaggtg gttttgccta tatcttgctg tttgaggaca agttgattgc ggctcttgac
     9301 cctaatggct ttcgtccgct ttctatcggg aaaatggcca acggagcggt ggttgtttcc
     9361 tctgaaacct gtgcttttga ggtcattggt gccgagtgga ttcgtgattt gaagccaggt
     9421 gagattgtga tcattgatga cgagggcatt cagtatgaca gctatacaga tgatactcag
     9481 ttggcgattt gttctatgga gtatatctac tttgctcgcc ctgattctaa tatccacggt
     9541 gtcaatgtcc atacggcacg taagagaatg ggagcgcaat tggcgcgaga atttaagcat
     9601 gaggcagata ttgtagttgg tgtgcccaat tcttccctaa gcgcggctat gggatttgcg
     9661 gaagaatcag gcttaccaaa tgaaatgggt ctgatcaaaa accaatacac ccagcgaact
     9721 tttatccaac cgactcaaga attgcgggag caaggagtgc ggatgaaact gtctgctgtt
     9781 tcgggtgttg tcaaaggcaa acgtgtggtc atggtggatg attccattgt acgtggaaca
     9841 acctctcgtc gtatcgttca gctcttgaaa gaagcgggtg cgactgaggt tcacgttgcc
     9901 attggaagtc ctgcactagc gtatccatgt ttctacggga ttgatatcca gacccgtcag
     9961 gagctgattg cagccaatca tacggtcgaa gaaactcgcc aaatcattgg tgcggacagt
    10021 ctgacttatc tttcaattga tggcttgatt gagtcgattg gtatcgaaac agatgcgccg
    10081 aacggtggtc tctgtgtcgc ttactttgac ggtgactacc caacgcctct ctatgactac
    10141 gaagaagact atcgtagaag tttggaagaa aagaccagtt tttacaagta ggcgacagat
    10201 tctccattaa agaaaaggaa aaaacaaatg acaaataaaa atgcatatgc ccagtcgggt
    10261 gtggatgttg aagcgggtta tgaagttgtt gaacggatta aaaagcacgt ggcccgtacg
    10321 gagcgtgcag gtgtcatggg agctcttggt ggctttggtg gtatgtttga cctttccaag
    10381 actggggtta aagaacccgt cttgatttca gggactgacg gtgtcggaac caagctcatg
    10441 ttggctatca agtacgacaa gcacgatacc atcgggcagg actgtgtggc catgtgtgtc
    10501 aacgacatca ttgctgcagg tgcggaaccc ctctattttc tcgactacgt agcgacaggg
    10561 aagaatgaac cagctaagct agaacaagtg gttgctggtg tggcagaagg ttgtgtgcag
    10621 gctggtgctg ccctcatcgg tggggaaacg gctgaaatgc cgggcatgta cggcgaagac
    10681 gactatgact tggctggttt tgcggtcggt gtggctgaaa aatctcaaat cattgacggt
    10741 tcaaaggtgg tagagggaga tgttcttctc ggacttgctt caagtgggat tcactcaaat
    10801 ggttactctt tggttcgtcg tgtctttgcg gattacacag gtgaggaagt cctaccagaa
    10861 ttggaaggca agaaacttaa ggaagttcta cttgagccga ctcgtatcta tgtcaaggct
    10921 gtcttgccgc tcatcaaaga agagttggtc aacggcattg cccacatcac aggtggtggc
    10981 tttatcgaaa atgtccctcg tatgtttgca gatgacctag ctgctgaaat tgatgaaagc
    11041 aaagttccag tgcttccaat tttcaaagcc cttgaaaaat atggtcagat taaacacgaa
    11101 gaaatgtttg aaatcttcaa tatgggtgtg ggacttatgt tggcggtcag ccctgaaaat
    11161 gtagagcgtg taaaagaatt gttggatgaa gcagtctatg aaattggtcg catcgtcaag
    11221 aaagaaaacg aaagtgtcat tatcaaatga aaaaaatagc ggtttttgcc tctggtaatg
    11281 gctcaaattt tcaggtgatt gccgaagaat ttccagtgga gtttgtcttt tcagaccatc
    11341 gtgatgccta tgtgcttgag cgtgcaaagc agctcggcgt tctgtcctat gcttttgaac
    11401 tcaaggagtt tgagagcaag acagactacg aagcagccct tgtcgaactc ttggaagaac
    11461 accagattga cttggtttgc ctagcaggct acatgaaaat cgttggacca accttattgt
    11521 cggcttatga aggtcggatt gtcaacattc atccagccta cttgccagaa tttccaggag
    11581 ctcatgggat tgaggatgct tggaatgctg gcgtgggtca gtctggtgtg accattcact
    11641 gggtggattc gggtgtggat acaggccagg tcatcaaaca ggttcgtgtg ccacgactag
    11701 ctgatgatac cattgacaga tttgaagctc gcatccatga agcagagtac aggttgtatc
    11761 cggaagtagt gaaggctcta tttacagatt gactttttga tgattcatat gatatctttg
    11821 attttaaatt ggagtcagtg tttgttgaag acggcttcaa acggaggtat ttgtaatgtt
    11881 agaatctaaa aaaacaactc gatatgtatt ttatgtctat ctgatgttat taacttgggg
    11941 aatcttattt aagtttgaaa caaatcctga atttatagca tttttcttag ctccaaggta
    12001 tatcaattgg attccatttt cagaaccact aatagtcgat ggaaaaattg tttttgctga
    12061 aatgttattt aatctgattt cctttattcc attaggtgtt tgtttccctt tgataaaaac
    12121 taatttatct agtttaagaa tagtcgggac aggtttcttg attagtttat tgtttgagtg
    12181 cttacagtat attttagcaa taggtataac agatataacg gatttgactt taaatacgct
    12241 aggtgtctgt gtaggcttac tgatttatca aatttttata agagtgttca aatcacagac
    12301 tagaaaatgg atcaatatct taggtatgct tagccttggt tttgcttatc ttgttttact
    12361 gttactgcat ttaattggtg tttaactaat gattaaaaag gagaatataa tgactaaacg
    12421 cgtcttaatc agcgtctcag acaaagcggg cattgttgaa tttgcccaag aactcaaaaa
    12481 acttggttgg gagattatct caacaggtgg aactaaggtt gcccttgata atgctggggt
    12541 ggataccatt gctatcgatg atgtgactgg tttcccagaa atgatggacg gtcgtgtgaa
    12601 gaccctccac ccaaatatcc acggagggct tctcgctcgt cgtgacttgg atagccactt
    12661 ggaagcggct aaggacaaca agattgagct cattgacctt gtggtggtca acctttaccc
    12721 atttaaggaa actatcctta aaccagatgt gacttatgct gatgcagttg aaaatatcga
    12781 tattggtggg ccatctatgc ttcgttcagc agcgaaaaat catgccagtg ttacagttgt
    12841 ggtagatcct gctgactacg ctgtggtttt ggacgaattg gcagcaaacg gcgaaacctc
    12901 ttatgaaact cgccaacgtt tagcagccaa agtatttcgt cacacagcgg cttatgacgc
    12961 cttgattgca gaatacttca cagctcaagt gggtgaaagc aagcctgaaa aactcacttt
    13021 gacttatgac ctcaagcaac caatgcgtta cggtgagaat cctcaacaag acgcggactt
    13081 ttaccagaaa gctttgccta cagattactc tattgcttca gccaaacagc tcaacgggaa
    13141 agaattgtca tttaataata tccgtgatgc agatgctgct atccgtatca tccgtgactt
    13201 caaagatagt ccaaccgttg tggctctcaa acacatgaat ccatgtggaa ttggtcaagc
    13261 tgatgacatc gagactgctt gggactacgc ttatgagtct gacccagtat ctatctttgg
    13321 tgggattgtc gtcctcaacc gtgaggtgga tgctgcgaca gctgagaaga tgcacggcgt
    13381 tttcctcgaa atcatcattg ccccaagcta tacggatgaa gcgctagcta ttttgatcaa
    13441 taaaaagaaa aacttgcgta tccttgcctt gccatttaat gctcaagagg ctagcgaagt
    13501 ggaagcagaa tacacaggtg tagtcggtgg acttctcgtg caaaatcaag acgtggtcaa
    13561 ggaaagccca gctgactggc aagtggtgac caaacgtcag ccaactgaga cagaagcgac
    13621 tgctcttgag ttcgcttgga aggctatcaa gtacgtcaaa tcaaatggta ttatcgtgac
    13681 caacgaccac atgacacttg gtgttggtcc aggtcaaacc aaccgtgtgg cttctgttcg
    13741 ccttgccatt gaccaagcca aagatcgtct gaacggggcg gtccttgctt cagatgcctt
    13801 cttcccattt tcggataacg tggaagaaat cgccaaagca ggaattaagg ccatcatcca
    13861 gcccggtggc tctgtccgtg accaagaatc catcgaagca gcgaataaat acggcttgac
    13921 tatggtcttt acaggtgtga gacattttag acattaagaa gataaaaggg aagaaaacag
    13981 tttctttcct tttttggctt aaaatgctaa ctgaaacaag attaaaacga acttttttga
    14041 tataatgttg gtaaataatt cgcaaaagag gttgaggaat gaaactgctt gttgtcggtt
    14101 ctggtggtcg tgagcatgcg attgctaaaa agttacttga atcaaaagac gtggaaaaag
    14161 tctttgtagc tcctgggaat gatgggatga ctctggatgg tttggaattg gtaaatatct
    14221 ctatttccga acattctaaa ttgattgact tcgcaaagac caatgatgtt gcttggacct
    14281 ttatcggtcc agatgatgcc cttgctgctg gtatcgtgga tgattttaac caagctgggc
    14341 ttaaggcctt tggtccgact agggctgcag cggagctgga gtggtccaag gattttgcca
    14401 aggaaatcat ggtcaaatat ggtgtttcga cagcagccta tggcacattt tcagatttcg
    14461 aggaagccaa ggcctatatc gaaaagcatg gtgcgcctat cgtagtcaag gcggatggct
    14521 tggcgcttgg gaagggtgtc gtcgttgctg agacggttga gcaagcggtc gaagccgctc
    14581 atgagatgct tttggacaat aaatttggtg actcaggtgc gcgcgtggtt attgaggaat
    14641 tccttgaagg agaggaattt tcactctttg cctttgtcaa tggtgataag ttctacatca
    14701 tgccaacggc tcaggaccac aaacgtgcct acgatggcga caaagggcct aacacgggtg
    14761 gtatgggtgc ctatgcgcca gttccacact taccacagag tgtagttgat acagcggttg
    14821 acaccattgt caagccagtt ctagaaggga tgattaaaga aggtcgccct tatctaggtg
    14881 tcatttacgc agggcttatc ttgacagcag acgggccgaa ggtcatcgag ttcaactctc
    14941 gatttggaga tcctgaaact cagattatat tgcctcgttt gacctctgac tttgctcaaa
    15001 atatcacaga tatcctggat agcaaggagc caaatatcat gtggacggac aagggtgtga
    15061 ctctgggtgt ggttgtcgca tccaagggct acccgctaga ctatgaaagg ggcgttgagt
    15121 tgccagccaa gacagaaggc gatgtcatca cctactatgc aggggctaag tttgcggaaa
    15181 atagcagagc actgctctca aacggcggac gagtttatat gctggttacc acagtagcca
    15241 ccgtcaaaga agcccaagcc agcatctacc aagaactatc ccaacaaaaa atagaaggac
    15301 tcttctaccg aacagatatc ggaagcaagg caattaagta aagatataag aataacgcga
    15361 cgcagtcgcc aaacacgata atggtcgtcg tggtgaaaag accagaacag tgaatgttct
    15421 ggtcaggggg aaactttgag accttaggct caaagtttag gaatgaaacc gaaggtttgc
    15481 ttccgcctcc atcacctaag accattatca aaaagaaaaa taaaaattca caaaatacgt
    15541 taatggtcgt atggtttgcg agcgttagcg agcaaatata gaataatcac cgccgttgtg
    15601 aaagaacgat tggatgataa tccaatcgtt cagggaaatt ggaagacctt gggtttccaa
    15661 tttaggcatg agacaccttt ggtggctgct gccgtccctc acaagctaag gtgattgttg
    15721 aaaaagagga aaaaggagaa gaaatgaaac cagtaatttc catcatcatg ggttcaaaat
    15781 ccgactgggc aaccatgcaa aaaacagcag aagtcctaga ccgcttcggt gtagcctacg
    15841 aaaagaaagt tgtttccgca caccgtacac cagacctcat gttcaaacat gcagaagaag
    15901 cccgtagtcg tggcatcaag atcatcatcg caggtgctgg tggcgcagcg catttgccag
    15961 gcatggtagc tgccaaaaca acccttccaa tcattggtgt gccagtcaag tctcgtgctc
    16021 ttagtggagt ggattcactc tattctatcg ttcagatgcc gggtggggtg cctgttgcga
    16081 ccatggctat cggtgaagct ggagcgacta acgcagctat ctttgccctc cgtctcctct
    16141 ctgtagaaga taagtccatt gcggatgcac ttgccaactt tgctgaagaa caaggaaaaa
    16201 tcgcagagga gtcgtcaaat gagctcatct aaaacaatcg gaattatcgg tggcggtcaa
    16261 ctgggtcaga tgatggccat ttctgctatc tacatgggcc acaaggttat cgcgctggat
    16321 cctgcggcgg attgcccggc ctctcgcgtg gcggaaatca ttgtggcacc ttataacgat
    16381 gtggacgccc tccgtcagtt ggcagaccgt tgcgatgtcc tcacttatga gtttgaaaat
    16441 gtcgacgctg acggtttgga tgccgttatc aaggatggac aactccctca aggaacagat
    16501 ctgctccgca tttcgcaaaa tcgtattttt gaaaaggact ttttgtcaaa caaggctcaa
    16561 gtcactgtgg caccctacaa ggtcgtgact tctagcctag acttggcaga tatcgacttg
    16621 tcgaaaaact atgtcctcaa gactgcgact ggtggctacg atggtcatgg acaaaaggtt
    16681 attcgttcag aagcagactt ggaagcagcc tatgcgctag cagactcagc agactgcgtc
    16741 ttggaagaat ttgtcaactt tgaccttgag atttctgtca tcgtgtcagg aaatggcaag
    16801 gaggtgacgt ttttcccagt tcaggaaaat atccaccgca acaatatcct gtctaagacc
    16861 atcgtaccag cccgcatttc tgaaagtcta gtagacaagg ctaaagctat ggcagtgcga
    16921 atcgcagaac aactcaactt gtctggaact ctctgtgtgg aaatgtttgc gactgatgat
    16981 gacgttattg tcaatgaaat cgccccacga ccacataact ctgggcacta ttctattgaa
    17041 gcctgtgatt tctctcagtt tgacacccat attctcggtg ttctcggagc accattacca
    17101 gtcatcaaac tccatgcgcc tgccgtcatg cttaacgttc tcggccagca tgttgaggcc
    17161 gctgaaaaat atgtcacaga aaattcaagc gcccacctcc acatgtatgg taaaatagaa
    17221 gcgaagcaca accgcaagat gggacatgtg actttgatta gtaatgaacc agatagtgtg
    17281 gatgagcttg gggaagggat tgatttttag gacaagtcta tgatacaaat tatcgttaat
    17341 acatttattg aaaaggataa gactggagca gttgttgaag tgttgtatgc cagtgctgac
    17401 caagataagg tacaagctaa atatgaagaa ctagctgcac aataccccga aaattattta
    17461 gctatctatg atgtaccgct ggatacggat ttgaatacac tagatcatta cccgtctgtg
    17521 tttattggaa aagaggagtt tgagtagaaa tcttggttta cctagatagc ttattcccaa
    17581 cagcttaaga agaaaggaaa aattaacaca tgatcaaccg ttactctcgc cctgagatgg
    17641 cgaatatttg gagtgaagaa aataaatacc gtgcttggct tgaggtggaa atcctctctg
    17701 acgaggcatg ggctgagttg ggggaaatcc ctaaggaaga tgtggctttg attcgcaaga
    17761 aggcggactt tgacatcgac cgtattttgg aaattgagca ggagacgcgc cacgatgtgg
    17821 tggctttcac gcgtgcggtt tctgagactc ttggtgaaga gcgcaagtgg gttcactatg
    17881 ggttaacttc tactgacgtg gtggatactg cttatggtta cctctacaag caggccaacg
    17941 acatcatccg tcgtgacctt gaaaacttca ctaatatcat cgctgacaag gccaaggagc
    18001 acaagttcac catcatgatg gggcgtactc atggtgtgca cgctgagccg acaacctttg
    18061 gtcttaaatt agcaacttgg tacagcgaaa tgaaacgcaa tatcgagcgc ttcgagcatg
    18121 cggctgctgg tgtagaagct ggtaagattt ctggtgcggt tgggaacttt gccaatatcc
    18181 caccatttgt agaggagtat gtctgcgata aacttggcat ccgtgcccaa gaaatctcta
    18241 cacaagtcct tcctcgtgac cttcacgctg agtactttgc ggttcttgcc agcattgcga
    18301 cttcaatcga acgtatggcg actgagattc gtggtctaca aaaatctgag caacgcgaag
    18361 tagaagagtt ctttgctaaa gggcaaaaag ggtcttcagc aatgcctcac aaacgcaacc
    18421 caatcggttc tgaaaatatg actggtctgg cgcgtgtcat tcgtggtcac atgattacgg
    18481 cttatgaaaa cgtcgctctc tggcacgaac gcgatatttc tcactcatca gctgagcgta
    18541 tcatcacacc agatacgacc attttgattg actacatgct caaccgtttt ggaaatatcg
    18601 tcaagaactt gacagtcttc ccagaaaata tgatccgaaa catgaactcg acttttggtc
    18661 ttatctttag ccaacgggct atgttgacat tgattgaaaa aggcatgacc cgtgagcaag
    18721 cctatgactt ggtgcaacca aaaacagcct actcttggga caaccaagta gactttaaac
    18781 cacttcttga ggcagattca gaagtaacat cacgtctcac acaagaagaa atcgatgaaa
    18841 tcttcaaccc agtttattac accaaacgag tggatgatat ctttgaacgt cttggactag
    18901 gtgattaatt aaaaaataaa cagcgagctt caatctcgct gtttattttt tatcgaaaag
    18961 acttagtctt cttttctttt agtgagtcca taggctgcta gtgtggacat gagtcctgcg
    19021 actactagtc ctgcagaatc gtgagttcct gtttcaggaa gttttttctc tgttaccaca
    19081 ggagctggat cttgaggaag agctttgctt tcctcagcag gagcagttga tggagctggt
    19141 tggcttggga tttctagttt tggtttttct tcagcaatag cggcttgtcc gttttcatcg
    19201 cctacatgtg ttaccatagt tccgacttcg actatttgag taacggcttc ctgtgctacg
    19261 acactattta caagtgtttt cacttcctta ccatcggcag aagtgctcac agagtagaag
    19321 ttgctacgat gtccattgac gcccttagta atgacttgtg tttttccttt gagtaagagt
    19381 ggattttcac gagtcactgt ggtaaatgga atttcttctt tttggatatc cagtctaggt
    19441 tttacctcag tagttggtgc aagaccactt tcatcaccct tgtgagttac aggagcgcca
    19501 acttcaacca cttggtttat aacttctttg gttacctggc tatcaaggac tgtttctgtt
    19561 gtttttccat tttcagtgag tacagagatg taatgagttc gttcaccttt gactcctgct
    19621 gtgataatat tttcctgacc agctgggagg ttaggatttt ctttcttgat aacttcaaat
    19681 ggaatttctt cagttcttgt gatgagttct ggtctggttt caacattggc agccacttca
    19741 ttttcatcta ggcttcctga atgagttgca gctggtttga ggccttgaag agcggctttt
    19801 aggttggcta caagtgtgtc aagctcagct tgtttattac ggttgaggtt gtaatttaga
    19861 gctgttttag ctgcgtcaag ggcctcaaga ctttctttac tatatccttc taagtttgta
    19921 ggaattttag ctaattcttc gcggagagca ttataattag cacggaagta gtctttgttg
    19981 tggtctgcaa aggcagtcat gagttcaaag atttcctctt ccttgtactc agcgcttggt
    20041 ctatctgccc agattgaaag catacttcca actgttggaa gatctacttc aggatatttg
    20101 gtagaagcta gttgattgaa tggtgttttt ccagtattct caatagcttt cttgaggaaa
    20161 ccaccaccat cttctggttt ttgaccaaga acataatacc agtcaccgtt ggtattcaag
    20221 aacttataac ccttgcttgc taggtattga ggtgatgcga ggttgtatcc ccaccagcct
    20281 ttagaccagt aagaaatcaa gacatctttg tcaaactgaa catcgtcctt gtcttcatag
    20341 tagaagccat cgttgaaggc cattggttga agccctcttt ctttggccat agctgcgagg
    20401 gtgttggcat attcggcaaa tttgccatag agttgatacc acttgaggta gtaccagcct
    20461 tgggcactag tcgcatcgtt ggcgtattcg tcagtaccaa agttgaaaat ctttgtttta
    20521 cctgcaaaga agtccatgta tttaccgatg agggctttta caaagttcat cgcttcttcg
    20581 tttttcaagt ccatagttgt ttttgaaact ttatcaaagt gggcttgagg atttttaata
    20641 cctaattttt ccatggcaac cagcatagca tccatgtgac ctggactgtt aatagctggg
    20701 atgagaccga tgtccttaga tttagcgtat tcaattagct ctgttacttc tgcctgtgtt
    20761 agtgcagtac cgtttggatc gtcgtagtaa gctttagttc cttcgataat agctttttta
    20821 acgtcatcac tagcataggt ttttccgttg gcagtaatgg tcatatcatc gagtagaaag
    20881 cgaagtccgt catttcctag aaggagatgg acatcagaat atccgagatc actggccttg
    20941 tctacgatgc gtttgagttg gtccagagta aagtatttgc gtccagcatc gattgagatt
    21001 accttgtttt tggcaagttt ttcaacctca cgtttagctt cttcttcttt ttgagcttca
    21061 ggcgtgaggg tcaagttgtt gacagtttct tgaagtttag caatggcttg atcaatcgta
    21121 tcttgttggg cacggctaag gttgctatcg agagagcgaa tagctttttc agcttctttt
    21181 acggccgtga cgctttctgc agtataacgg ttcaggtctt ttggtacctc gttaagtgct
    21241 tgctctgcag attcataatc agctgcgaag tattcagcgt tggcatttgc aaaatgacgc
    21301 atgagtttga agaggcgtga tggtgaataa cgtgcagatg gagtgtcagt ccaagcagct
    21361 accataccac cgatgattgg gatatcagct ccttctgttt ttggtacaga agtgattggt
    21421 gtgtttttaa taccattgag cccctgatcg agattgtacc agccttggcc atcagcgttt
    21481 cgtccaagaa cgtagtacca agcatcattg gtattaagga tttggtgacc tttttcagct
    21541 agtagtttag aagaagcgac atcgtagcct ccccaaccac cagtccacat agaaacgatg
    21601 atgtctttgt caaaactacc aaagcttgtg tcgctattgt agtagatacc gtcgttaaag
    21661 gccattggtt tgagaccgtg cgattttaca atacgagcga ggtcattggc gtaggcaata
    21721 aatttttcat agccttttac agggtagcct tcgtttggat agtatttatc agcttgaagc
    21781 acactccaac ctttagcatc tgtcgcatca ttggcatatt catcaagtcc gatgttgaag
    21841 atttcagtct ttttcgcgaa ataagcagca tacttgtcga taagggcttt tgtaaaagcg
    21901 acagcttgtt cgttgtcaag attgacagta cgggctgatt ccttcccaaa atagctaaag
    21961 ttagggtttt ggattcccaa ttctttcatg gcattgagaa tcgcatccat gtgaccagga
    22021 ctatttactg tcggaatgag accgatacct ttatctttgg catagttaat cagatctgtc
    22081 atttgacttt ctgttaagtg attgccgttt ggatcgttgt aataatcatt tgtacctttt
    22141 tcaatggcgc gtttgacatc gtcactggca taggtcttgc cgttagctgt gatgctcata
    22201 tcgtccaaca tgaaacggag tccatcattt ccgactaata ggtgtaaatc agtgtagcca
    22261 taatgtttcg ctttatcgat gatttccttg agctgttctg gtgagaaata tttacgtcca
    22321 gcatcaatag aaacaatttt ctttttcgct agtttttcat ttacagttgc agcacgttcc
    22381 tttcctgcct ctgtttccgg tttgtcagcc tctgctttcg cttcatcttt tttagctggt
    22441 ttatccttgt cagtcttgtc tgtatttgac tctttagaat caacctcttt cgcttcttcc
    22501 tttttagggc tagcttcttc tgccttttta ttagcagttt ctttttcagc agaagttgga
    22561 gttaccacct ctgctttatc actaggagtt gaactaactt cctcttgtgg tttttcttct
    22621 gtttttggaa gactagctac cttatcagta gctggagttt ctgtttctac agtttttgga
    22681 gcttctggtt gaagctctgc tttaggtgtt tcctcagtcc gattttcgga tgattgaggg
    22741 gaatcagaaa ccgtatggat ggtcggttgg ttttctgtag tagtaggagt aactccatcg
    22801 gctgcaacag tctgtgcttg gaaggcaaat ccaattagaa cagaagctgc tcctacagcg
    22861 tatttacgaa tagaaaaacg ctgttgtttt tcatgtttca ttgcaaaacc tcctgattgc
    22921 attgttatat tgatagcgat tatataaatc aacgccttta ttttatttct tatattaacg
    22981 agagtcaaga ggagatgaca aaaaactata ataagtataa aaaaatataa aatttaaact
    23041 taag
//
LOCUS       DAVKGU010000022        22042 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKGU010000022 DAVKGU010000000
VERSION     DAVKGU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.022"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(205..1428)
                     /locus_tag="VW077_000858"
     CDS             complement(205..1428)
                     /locus_tag="VW077_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9454906.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            1595..2917
                     /locus_tag="VW077_000859"
     CDS             1595..2917
                     /locus_tag="VW077_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9454907.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            2919..4805
                     /locus_tag="VW077_000860"
     CDS             2919..4805
                     /locus_tag="VW077_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9454908.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            4973..5317
                     /locus_tag="VW077_000861"
     CDS             4973..5317
                     /locus_tag="VW077_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9454909.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            5327..6739
                     /gene="lacG"
                     /locus_tag="VW077_000862"
     CDS             5327..6739
                     /gene="lacG"
                     /locus_tag="VW077_000862"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9454910.1"
                     /translation="MENLQFKVLPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKLGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            6808..8487
                     /locus_tag="VW077_000863"
     CDS             6808..8487
                     /locus_tag="VW077_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9454911.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDISVMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLVTSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGVESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLMLVVDILIYLPFCRAYDRQLLMKEYVASSNDIILEEETSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKSLQFVLEHYQAV"
     gene            complement(8614..10053)
                     /locus_tag="VW077_000864"
     CDS             complement(8614..10053)
                     /locus_tag="VW077_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9454912.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(10057..11406)
                     /gene="trkA"
                     /locus_tag="VW077_000865"
     CDS             complement(10057..11406)
                     /gene="trkA"
                     /locus_tag="VW077_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9454913.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11594..12442
                     /locus_tag="VW077_000866"
     CDS             11594..12442
                     /locus_tag="VW077_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9454914.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            12457..13005
                     /locus_tag="VW077_000867"
     CDS             12457..13005
                     /locus_tag="VW077_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9454915.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            13012..13269
                     /locus_tag="VW077_000868"
     CDS             13012..13269
                     /locus_tag="VW077_000868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454916.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            13371..15053
                     /locus_tag="VW077_000869"
     CDS             13371..15053
                     /locus_tag="VW077_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454917.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQIQLQTELVKETPALQSLFILEEVSFSYNDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVNEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQQGHTIVMITHDMQLMLDYSDRALVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            15038..15868
                     /locus_tag="VW077_000870"
     CDS             15038..15868
                     /locus_tag="VW077_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9454918.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLATMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            16061..16564
                     /locus_tag="VW077_000871"
     CDS             16061..16564
                     /locus_tag="VW077_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9454919.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      16618..16852
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            17017..17580
                     /locus_tag="VW077_000872"
     CDS             17017..17580
                     /locus_tag="VW077_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9454920.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            17594..18220
                     /locus_tag="VW077_000873"
     CDS             17594..18220
                     /locus_tag="VW077_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9454921.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            18247..18636
                     /locus_tag="VW077_000874"
     CDS             18247..18636
                     /locus_tag="VW077_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU9454922.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHFYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            18641..19089
                     /locus_tag="VW077_000875"
                     /pseudo
     CDS             18641..19089
                     /locus_tag="VW077_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            19217..19804
                     /locus_tag="VW077_000876"
     CDS             19217..19804
                     /locus_tag="VW077_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9454923.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            20194..21801
                     /locus_tag="VW077_000877"
     CDS             20194..21801
                     /locus_tag="VW077_000877"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9454924.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         6558 a         3733 c         4855 g         6896 t
ORIGIN      
        1 ttatttaagt tctgggggtt atgatgaatg tttttataat aataaaatgc agctagaaga
       61 aaagttattt taatataaat cactcaacat agcgagaagg cggtttccta tatggaagtc
      121 gtcttgtttt catattgtct aaatacgaga tatttactac aggttatagt tgatttaaac
      181 aggtgtatag atttctaata tatattaagg tgaaataata tcttgtaata cattgctatg
      241 ctctatgaaa aaagcaacga tagctaatgc acaagctcct atacttccac gatgtttgtt
      301 gtaaggtaaa atttcaattt ctatattacg agtaaaggga acgaagtgga gctggtcttg
      361 gattttatcc ctgacttcat gagtgaaagg ttgataatta agcaattgac tgttgatata
      421 gattttatca gcaggattga tgaggagaag atttgcaata gaagtggcca ataaattgac
      481 tcctttatca atttgttggc gtaaagcgga gtcgcctaaa ttataagccg ttaaaagggt
      541 gtctaaatga atgtcttttt cagtctttac aaggcttttt agtacagtta gttgggaatt
      601 tttaaataat aattgggcgt gtttgattaa ccaagcatcc gaaatatatg tttgtaaaca
      661 accttttttt ccgcattcac aatattgccc attcaattcg acaatggtgt gtccaatttc
      721 tccgatataa ggatttttag aggctcctat tttttctttt gtgaaaaagg aagtgtaaat
      781 acctaatcca gcgtgtagga aaataaagtt atcgggggta ttgtgtggat taaaaaggta
      841 ttgtcctata gccatacaat cgatattatt ttttacaata aaaggaaaat tgaatctttt
      901 aattacattt aataaattaa aagattccca tatggggttg tttgtaatga tacttccgga
      961 gtctttgtta taatgtccag gtattgataa gccaaatcca agtaattttg tttccaaagg
     1021 gacgtactct atagctgttt ttatcatatt ggtaatggtg gaattgattt ctgaaatagg
     1081 ataatcttca ttcaaaattt ctgtctcgaa aagagttttc ccttgtaagt cacatagact
     1141 gagaaagata cctaggacgt ttatttcaat acctccaaca acatacggaa tattatctgt
     1201 gatagaaata gtttttctac gtcttccaga tccggaaaac tcgctaaatt catcaccagt
     1261 ttcgtatatg agggactgtt cgcttagctc ttttatgatg ttagtcgttg ttgctggagt
     1321 aagtcctgtt tcattaacaa tatctgtacg ggatgttgct ggatttcgat atagaaactc
     1381 cagtatagtg gctctatttt gtaaaaaggc tgattgtttt gtaatcattt gtttcctcct
     1441 ttggtttatt ttatattaaa cataagaaaa aatcaattaa aattattatt ctatacaaag
     1501 aaaaaatgtt gtaaaaacaa caaaaaatat attgacaacg ctttctgtta gtggtatatt
     1561 taatttataa aaaataaaaa aaggagttcc ggtgatgaac aatattttag cgtttttaga
     1621 aacaaaagtc gctccgtttg gtgaaaaagt tggcaaccaa cggcatttga aagctattcg
     1681 tgaaggattt atgatggcaa tgcctttgat tttagtcggc tctttatttc ttattctaat
     1741 cagttggcct caagaggatt ttacaaattg gctgaatagt gttggattgc taagtatctt
     1801 gacaactatg aatcagtcaa cagtagcgat tatctccttg gtcgcttgtt tcggtattgc
     1861 ctacaggttg tcggaaggat atggtacaga tggtccgtcg gcagggatca tagccttatc
     1921 cagttttgta ttgatggcac ctcgtttttc gagtatggtt tatgataaaa atggggagca
     1981 ggtcaagcag ttatttggcg gcgcaatacc attttctagc ctgaatgcat cttctttgtt
     2041 tatggcgatt actattggat tggttacagc agagatttat cgtatgttta tccagcgcgg
     2101 aattacgata aaaatgccaa gtggtgtccc agatgtggta agtaaatcat tttcagctct
     2161 tttacctggc tttactactt ttgttttgtg ggctctggtc ttaaaaggtc ttgaagcggc
     2221 aggagttgca ggaggtctca acggactcct aggtgcaatt gttggaacac cgcttaagtt
     2281 aattgcagga acgcttccag gtatgattct atgtgttatt gtaaactcat tcttttggtt
     2341 ctgtggagtt aatgggggac aagttttaaa tgcttttgta gacccagttt ggttacaatt
     2401 tactacagaa aaccaagaag ctgtggctgc aggacaaaca ctccaacaca ttattacatt
     2461 accgtttaaa gatttatttg tatttattgg tggcggtgga gcgactattg gtcttgcgat
     2521 ttgtctcttc ctatttagta agagtcgtgc gaataaaaca ttaggtaagc tagctattat
     2581 accgtctatt tttaatatca atacagctat tctatttacg tttccaacag ttttaaatcc
     2641 gattatgctg attccgttta ttgctactcc tacaatcaat gccttgatta cctatgtatc
     2701 aatggctgta ggattagtac cctatacaac aggtgtaatc cttccgtgga caatgccacc
     2761 gattatagga ggcttccttg caacaggggc tagttggcga ggagctctat tacaagttgt
     2821 tttgattttg gtttctgtag caatttatta tccattcttc aaaattgcag ataaacgcaa
     2881 tcttgaaaaa gaaaaagcta ctgttggagg gaaataagat ggttatcaga gtatttgatc
     2941 aacagaaaaa tacttattct agctttgcct tagaggaatt aagttactat atgaatcggg
     3001 tctttaagac taacatagag cttgtcgagg agaaggaagc ggatattttt gtaggattag
     3061 tcaataaaga ggacagaaaa gaccatgttc ttatctcatt agacaagggt aaggggagaa
     3121 ttgagtctaa tacaattgta ggtttactta ttggaattta ccgaatgttt catgaatttg
     3181 gggttgtgta tactagacca gggcgcggac atgactttgt tccagagtta cgatttgaag
     3241 attttttaga taaacagcta tctatagatg aaacagccag ttactatcat aggggagtat
     3301 gtatagaggg agcggattca tttgaaaata tactagattt cattgattgg ctacctaaga
     3361 ttgggatgaa cagttttttc atccagtttg aaaatcctta ctcttttttg aaacgttggt
     3421 atgaacatga atttaatcca tatctaaata aagaacaatt ttcaaatgaa ttagtacaag
     3481 aattgagtga taggttggat aaagaattgc aaaaaagagg tcttattcat catcgtgttg
     3541 gtcatggatg gacaggtgaa gttttaggtt actcttcaaa atttggctgg gaatcaggtc
     3601 ttagtatttc agaggagaag aaaccctatg tcgctgaaat aaacgggaaa cgagaattgt
     3661 ttaatacggc tccgatttta accagcctgg atttttcaaa tccagatgta gctgataaga
     3721 tggtagaaat tatcaaggat tatgccaaga aaagacctga tgttaactac ttacatgtat
     3781 ggttgtcgga tgctcgtaat aatatttgtg aatgcgaaaa ctgtagacaa gaattggttt
     3841 cggatcagta tattcgtatt ctcaatcaat tggatagggc tttaacgagt gagggattag
     3901 atacaaagat ttgttttctg ctttatcatg agttgttatg ggcacctcag aaagaaaaat
     3961 tagataatcc tgaacgcttt accatgatgt ttgcaccgat tacaagaaca tttgaaatga
     4021 gttatgcaga tgtagatttt gataattcca tacctacgcc taaaccttat atgcgtaata
     4081 aaattatact tccgaattct cttgaggaaa atttatctta tctttttgag tggcaaaaag
     4141 catttaaagg agatagtttc gtatatgact atcctttagg gcgtgctcat tatggcgatt
     4201 taggctatat gaaaattagt caaactattt acagagatgt atcttatctt tccaacctac
     4261 atttgaacgg gtacatttcg tgtcaagaat tacgtgccgg attccctcat aattttccta
     4321 attatgtcat ggggcaaatg ctctggaaga agacaagaag ttatgaagaa ttgattgaag
     4381 aatacttttc tgctttgtat ggggaaaatt ggcagtctgt tgttgaatat ttagaaaaat
     4441 tatccattta ttcctcttgt gattatttta atgcaattgg cagtcgtcaa aatgatgttt
     4501 tagcgaatca ttattatata gcttacaatc tagctgataa ttttttacca attattgagg
     4561 aaaatatttc taagttatta aatagtcaaa aggatgaatg gaaacagctc agttatcatc
     4621 gtgaatatgt tgttaagatg gcgaaggctt tatatcttca agcaactgga aaaacaaggc
     4681 aagctcaaga tgaatggaga aatgtgttga attatatccg tgggcacgaa ttgctatttc
     4741 aatctaattt ggatgtttat cgtgtaattg aagtagcaaa aaattacgct ggtttccact
     4801 tataaatcat aagtatagaa aatgaactaa ggtattcaga gaagattgac cctaaatatt
     4861 atgaaattta aggattttta agatatttag ggtcgacttt ctatttgtat cgtagcgaag
     4921 tcattttaat aatgatgtgt aaaagatgga tcaagattga ggaggaagaa agatgaaatc
     4981 aaaagaagaa ataaatatgc ttggttttac aattgtcgct tacgcaggag atgcaaggtc
     5041 agatttgatg gatgctttgg cgtttgcgag agatggatat tttgaacagg caagagaatt
     5101 ggttgagtct gcaaacgact caatagtgtc tgcccatcga gaacagacta atttattagc
     5161 ggaggaggca tatggagata attttgaagt gagctttatt atgattcatg gtcaagatac
     5221 tttgatgaca acgatgctat tgtatgatca ggtaaagttt tttattgatg aatatgaacg
     5281 aattcgaaag attgaagaac atattggttt gcaatgagga ttagtcatgg aaaatttaca
     5341 gtttaaagtc ttaccgaagg agtttttatt aggagctgct accgctgctt atcaagtaga
     5401 gggtgcaact agggtagatg gcaaaggaat aaatatgtgg gatgtttatt tgcaagaaaa
     5461 tagtccgttc ttaccagatc cagctagtga tttttattat cgttacgaag aggatatagc
     5521 tttggcggca gaacatggtt tgcaggcttt gcgtttatct atttcttggg ttcgtatatt
     5581 tcctgatata gatggagatg ctaatgtatt agctgttcat tattaccata gagtttttca
     5641 gtcttgctta aaacataatg tgattccgtt tgtttcttta catcattttg attcgcctca
     5701 gaaaatgtta gaaacagggg attggttgaa tagagagaat attgatcgtt tcatacgata
     5761 tgctcgcttt tgtttccaag aatttacaga agtcaagcat tggtttacaa tcaatgaact
     5821 gatgtctctt gctgcaggtc aatatatagg aggtcagttt cctccaaatc atcattttca
     5881 attatctgaa gcaattcaag cgaatcataa tatgttgttg gcgcatgctc ttgcagtcct
     5941 cgaatttcat caattaggga ttgagggaaa ggtaggttgt attcatgctt taaagctagg
     6001 ctatcctatt gatgggcaaa aagaaaatat tttggcagct aaacggtatg atgtttataa
     6061 taataaattt ctattagatg gaactttttt gggctactac agtgaggaca cgctttttca
     6121 cttgaatcaa atattggaag ctaataattc tagctttatt attgaagatg gtgatttaga
     6181 aattatgaag agagctgcag ctcttaatac gatgtttggg atgaattatt atcgttcaga
     6241 atttattcgt gaatacaaag gtgaaaatag acaagaattt aattcaacag gaataaaagg
     6301 acagtcttct tttaaattaa atgctctagg tgaatttgta aaaaaacctg gtattccgac
     6361 aacagattgg gattggaata tttatcctca agggttattt gatatgttgc ttcgtatcaa
     6421 agaagaatat cctcaacatc cggtcattta tttaactgaa aatggtacag cccttaaaga
     6481 agttaagcca gagggcgaga atgatattat tgatgacagt aagagaatcc gttatattga
     6541 gcaacattta cacaaagttt tagaggctcg agatagggga gtcaatattc aaggctattt
     6601 tatatggtct ttgcaagatc aattttcttg ggcgaatggc tacaataagc gatatggtct
     6661 tttctttgtt gattatgaaa cacagaagag atatattaag aaaagtgctc tttgggtaaa
     6721 agggctaaaa cggaattaag gttagcgatt tgactgatgt ttaatatatt ttaactatga
     6781 gcttaaattt tttataagag gagttttatg gataagctag tcgctgccat tgaaaagcaa
     6841 caagggaaat ttgaaaaaat ttctactaat aactatatga tggctattaa agatggattc
     6901 attgctacta tgcctttaat tatgttttca agctttttga tgattattat tatgattcct
     6961 aaaaatttcg gagtagagtt accgagtcca gctattgtct ggatgagaaa agtgtatatg
     7021 ttaaccatgg gagttttggg tgttattgtt tcagggacag ttgcaaagtc attggctgga
     7081 aatgttaata gaaaaatgcc tcctggaaag gtaataaatg atatttctgt aatgttggca
     7141 gccatatgta gttatctggt attaactgta acgcttgtag ttgatgagaa gacgggatct
     7201 acaagtttgt cgacaaacta tttaggatct caaggattgg taacttcgtt tgtcagtgcc
     7261 tttattactg taaatgttta ccgattctgt attaagcgag acattactat tcatttacct
     7321 aaggaagttc ctggggctat atcacaagct tttagagata tttttccttt ttcttttgtt
     7381 ttacttatta gtggtttgtt agatattgta tctcggttta gtttagatgt tccttttgcc
     7441 caagtatttc aacaactatt gactcctatt tttaaggggg tagaatcata tcctgctatg
     7501 atgttgattt ggtttatgtg tgctttgctt tggtttgttg gaattcatgg accatctatt
     7561 gtcttacctg ctgttacagc tttacaactg agcaatatgg aagagaatgc tcaacttctt
     7621 gcaaatgggc agttccctta tcattcttta acacctaatt tcgggaatta tatcgctgct
     7681 attggaggaa cgggggctac ctttgttgta ccatttattt tgattttctt tatgcggtct
     7741 aaacaattaa aatcggtagg taaagctaca attactcctg ttttatttgc ggtaaatgaa
     7801 cctcttctat ttggtatgcc tgttattttg aatccctatc tttttgtccc ttttttgatg
     7861 actccaccag taaatgtatt tctaggaaag gtctttattg atttctttgg aatgaatgga
     7921 ttttatatcc agttaccttg ggcctttcct ggtcccttgg gattgttaat tggaacgaat
     7981 tttcaatcta tctcctttgt atttttatct ttgatgttag ttgttgacat attgatttat
     8041 ttgccattct gtagagcgta tgatagacag ttactgatga aagaatatgt tgcaagctca
     8101 aatgatatta ttttagagga ggagacaagt gaaataatgc ctggtgagat agatgtaata
     8161 aaaagtaagg agttgaaagt actagttctt tgtgcagggt ctggaacaag tgcgcaatta
     8221 gccaatgcaa ttaacgaggg cgctcaatta gcagaagtta gagtgattgc gaattcagga
     8281 gcgtacggag ctcattatga tattatgggt gtttatgatt taattattct ggccccacaa
     8341 gttcggagtt attatagaga gatgaaggtg gatgcagaaa gattaggtat tcagatagtt
     8401 gctaccagag gaatggaata tattcattta acaaagaatc caagtaaatc cttacaattt
     8461 gtattggagc attaccaagc tgtgtagtaa gtttttccat cttttatttg agtaaggact
     8521 ttgcttacag acaggcttgg atttaaaaac gttccccctt ttttaatata agaatccctc
     8581 tttttacaat tgtaaaaaga gggattttgt attttatctc ttagaccaag ttctcttcat
     8641 aaagagaagg aggattgggt aaatctccaa gcgccctgca atcattgcaa aggataggag
     8701 aatttttgag atgggactaa agattgagaa actagaagtg gttcctagaa taggcccgat
     8761 attattgaaa cagctaaaga cagcgctggt cacgaccaga aaatcattgc tatctaggct
     8821 gacaataaag ataagcgcta gcaaaatcat agcatagatg acaaagtact tgagaatctt
     8881 atgctgggta tctttgtcaa tcaccgtttt attaacatgg agggtcaaaa cacggtgggg
     8941 cgataggatt gacaaaattt ggtttttggc aatttttgaa aggatgaggc ctcgaataat
     9001 cttgagtcca cctgcagttg atccagcaga gccaccgatt gccatgagga aaaggaggat
     9061 aaactgggag aagaggggcc agttggtaat atctccatat ccaaaaccag ttgttgtaat
     9121 gatgttggaa acctggaaga aggtcatttc aaagctcttt gaaaaccctg ggtagaggta
     9181 gagggtgttg aggctaatca agcctgtaga aaccagtaca atgaccaagt aagccctaag
     9241 ctcttcatct ccaaagaagg ccttgatgcg acggagcatg aggtagtagt agaggttgaa
     9301 atttactcca aaaaccagaa ctccgatact gaccagatag gtaatcagtg agctgccata
     9361 gtgggcaatt ccgtcgttat agacggtaaa gcctccagtt cccgctgtcc ccatagcaat
     9421 gacaaaacta tcgtagagag gcataccagc tagataatag atgatgacaa agagggagaa
     9481 gagagctaga taaaggagat agagaatctg ggcagtgttt tttagtttgg atacaacctt
     9541 gccaaaaaca ggacctggaa cctcagcctt catcacctct aggtggctat ttttggcatt
     9601 gtccataata gcaagtgcaa aaacaagcac tcccatccct ccaatcaagt gggtaaaact
     9661 tcgccagaag aggagggaac gactgagaac tgaaacgtcg ttcaaaatac ttgctccagt
     9721 agtcgtaaag ccggaactaa tctcaaaaaa ggcatcaata aggctgggga tttgcccaga
     9781 aaagacaaag gggagaccac caaagaaaga ccaaaggatc caacagaggg caacgatcaa
     9841 gactccctcc ttggcataaa tccgttgatt ttttggcttc tgtaaactcc ctgaaccgcc
     9901 taacaatacg agaatcccta tggtcgaaaa gagggctgta aagacttggc tcgattcacg
     9961 gtaatagaca gcgatagcca caggaaccaa gaggagaaca gcttcaatca aaagtaactt
    10021 tgaaaggagg taacgaatca tacttttatt catttcttac ctcgcaatca agtcataaat
    10081 cttggtgatg tttggcaaca aggttgttac taggagcttg tctccaactt ccaacatatc
    10141 ctccccagtt gggaaaatag tcttgccctt tcgaataatg gctgcaataa gaaccccttt
    10201 tttcaatttc agttgagaaa gaggtttggc agtcatttta ttggcttcct tgatatggaa
    10261 ttgcagggtt tcgatttggc cattggctag atggtgcatg gcttgaaggt ctgaatactg
    10321 ggcattaact cgaccacgaa taaagtgcat aatcgtatct acagcgatgc ttttaggtgt
    10381 gatgatactt gaaaaatcag gcgcattgat aatctcgagg agactggtac gattgacctt
    10441 agtaatattt ttctgtacac ctaccctgtc aaggaacata gatgtaatca gattttcctc
    10501 atcgactcct gttagagtcg caacggcatc atagtgttga gcactttctt ccagcaggat
    10561 atcttttgcg gttccatctc cttgaacgat gtagagattt gggaatttct cgctaaagaa
    10621 gctggcgatt tcaggattga tttcaatgac ttttgtatcg atacgactat ctttgagaat
    10681 accaagtaga taataggcaa ttctacctgc tccaacgatg agaaggctct tcacggcgcg
    10741 tgatttaaaa taattatgga agagtatcat atcgacacgg ttaccagtga caaagattct
    10801 atctttatcc tgtacagtca tgtcaccgct tggaatgata atttgatgat ccctctctat
    10861 cgcacagaca atgacattac caaatttttt acgaaaatca gaaatgggca tttggcaaag
    10921 accgctggtg gacttgacga caaattccat gaggctaacg cgtccaccag caaagcgttc
    10981 gacagacagg gcgttgggga agtcaatgat attcgcgata gcgcgggcag ccaagagctc
    11041 aggattaacg ataagagaaa aaccgagaat attcttttcc ttgaaataag agttagaata
    11101 ttcagggttc cgcacccgaa cgatagtttc tttagctccc attttcttgg ctagaactgc
    11161 tgcaatcatg ttgacttcat cgtgctcagt cagggcgata aagatatcac aatcttggac
    11221 gctggcttgc tcaagaatgg caaaatcggc cccgttacca aggataccaa tgatatcaaa
    11281 gcgactgaca atatgattga gaacagcttc gtcttgctca atcagcaaaa catcatgctt
    11341 ttctgcaacc aaggagcgac agagggcaaa accaactttt ccccctccga caaggataat
    11401 tttcataata aaacctactt tttcatgatg taactatcat accctttttc aagaaaaaat
    11461 gcacctacta gctaataaca agagctttta gtgaaaattc gctataaggt aaaactatac
    11521 cctaaccaat tgaaatagct attagcgact ttctctgaaa tatggtatga taaaggatat
    11581 acaaggagat aaaatgaata ataatttact ggtattacaa tcagactttg gtctggttga
    11641 tggtgcggta tcggctatga ttggagtggc tttagaagag tctccaacct taaaaatcca
    11701 tcacttgacg cacgatatca cgccttataa tatttttgag gggagctatc gtctctttca
    11761 gacggtggat tactggcctg agggaacgac gtttgtatcg gttgtcgatc caggtgtcgg
    11821 ttcgaaacgt aagagtgtag ttgccaagac tgcaaaaaat caatacattg tcacgccaga
    11881 taatgggacg ctttccttta tcaagaaaca cgttggcatt gtagccattc gtgagatttc
    11941 tgaggtggcc aataggcgtc aaaacacaga gcattcttat accttccacg gtcgtgatgt
    12001 ctatgcctat actggtgcta aactggccag tggtcacatt acttttgagg aagtagggcc
    12061 agagctcagt gtggaacaga ttgtagagct tccagtcgta gcgaccatca tagaagatca
    12121 tctggtgaag ggagccattg atattctgga tgtgcgtttc ggttcgcttt ggacctctat
    12181 cacacgggaa gaattttaca agctggaacc agaatttggt gatcgttttg aagtgaccat
    12241 ctatcatgct gatatgctgg tctatcaaaa tcaggttgtc tatggcaaat catttgcaga
    12301 tgtgagaatt gggcaaccca tcctttacat caactctctc tatcgtttag gtttggctat
    12361 caaccaaggt tcctttgcca aggcctataa tgtaggtgtc ggttcatctt ggaccattga
    12421 aataaagaaa atagaaggat aaaataggag aatagtatgg aaattaaatt tacaattaaa
    12481 caagttgttg ctgtcggaat tggcgctgcc ctctttgtcg tcatcgggat gatcaacatt
    12541 ccaacccctg ttccaaatac aagcatccag cttcagtatg cggtacaagc gctactttct
    12601 attatttttg gaccgattat cggtttgctt gtcgggttga ttggtcatgc aatcaaggac
    12661 tctcttgctg gttatggtct atggtggact tggattatcg ctagcggact ctttggttta
    12721 gttgtgggac tttttagaaa gtacgttcgc gtgatcaatg gtgtttttga ctggaaagat
    12781 attcttattt ttaacctcat tcaactactt gcaaatgccc ttgtttgggg tgtcttggca
    12841 ccacttggag atgttgtgat ttatcaagaa gcggcagaaa aagtatttgc acaagggatt
    12901 gttgcgggaa ttgccaatgg tgtatctgta gctattgcag gaactcttct cttacttgcc
    12961 tatgcaggaa cccaaactcg tgcaggaagt ttgaaaaagg actaatactc aatgaaaatc
    13021 aaagagcaaa ctaggaagtt agccgcaggt tgctcaaaac agtgttttga aattgtggat
    13081 agaactgacg aagtcagctc aaaacatggt tttgaggttg tagatgaaac tgacgaagtc
    13141 agtaaccata cctacggcaa ggcgaagctg acgtggtttg aagagatttt cgaagagtat
    13201 aaaatgatgg ggaaggctgg gcaactagtc tttttcgatg tttatagatt agttaggcaa
    13261 gtaagctagg ataggaatct ttttggcgct aagattttaa gagaagagac tgcaagaagg
    13321 gcttgattta tgataaaata gaagtctaag aagcgaatga agagagtaag atgaaagaag
    13381 ctataattga gtggaaggat ttctctttcc ggtatgaaac acaacaagaa ccgaccttgc
    13441 aagggataga cttgaccatt tacaagggag agaaagtctt aattgttgga ccatctggat
    13501 caggtaaatc taccttgggt cagtgtttga atgggattat tcccaatatt tacaagggtc
    13561 agacatatgg agaatttttg ataaagggtc aaacagcctt tgatatgagc atctatgata
    13621 agtctcatct ggttagcaca gttttgcagg atacagatgg gcagtttatt ggcttgtctg
    13681 tggcagaaga tttggcgttt gctctggaaa atgatgtgac agccctagat gagatgaaag
    13741 gtcgtgttta taaatgggct gaaaagctgg accttcttcc tttactggat cagcgtcctc
    13801 aggatttgtc aggtggacaa aagcagcgag tcagtctggc tggtgtcttg attgatgaaa
    13861 gtccgattct cttgtttgat gagccactcg ccaatctaga tcccaagtca ggtcaggata
    13921 ttatcgaatt gattgaccag attcataagg aagaggggac gacgactctg attatcgagc
    13981 accgtttgga ggacgttctg catcgccctg tggatcggat tgtcttgata aacgatggtc
    14041 gtatcctttt taatgggagc cctgaccagt tgcttgcgac tgatttattg actcaaaatg
    14101 gaattcgaga acccctttat ctaacgactc tccgtcaatt aggtgtggac ttagtcaagg
    14161 aagaacaatt agcagatctg gataacttgt ctatctcaaa agggcaaatc cagttacaga
    14221 cggaactggt aaaagaaacc ccagcattgc agtcactctt tatactagag gaagtatctt
    14281 tttcttataa tgatagaccg attttaaaat ccctacattt agatattaaa aagggtgaaa
    14341 agattgctat tgtcggaaaa aatggagcag ggaaatcaac tctagccaag gctataagta
    14401 gctttattca gacggaagga cgctatcttt gggaaaaaca ggatataaaa ggcgattctg
    14461 ttgcagagcg ggcggaacga gtaggctatg tgctacaaaa tcctaatcaa atgatttcaa
    14521 ccaatatgat ttttgatgag gtggctctag ggctccgttt gcgaggtgtg aatgagaagg
    14581 aaattgaaac gagagtctat gaaaccttga aaatctgtgg actttatgaa ttccgtaatt
    14641 ggcctatttc tgccctgtca tttggtcaga aaaaacgtgt caccattgct tcaattttgg
    14701 tcttaggcgc tgaaattatc ctcctagatg aaccgactgc gggtcaagac cagaagaact
    14761 atactgagat tatggaattt ctcgaagaac tgcatcaaca agggcatacc attgtcatga
    14821 ttacccatga tatgcaattg atgctggatt attcagatcg agcccttgtc atggtggatg
    14881 gagaattgat tgccgatact gttccagcca gtctgttgag cgatcctgag ctgttagtaa
    14941 aagccaatct aaaagaaacc tccatcttta acttggctaa gaaactagat gtggatccat
    15001 tggatttaac ggcattttac aaagagagga gagagggatg caagctaaat taatcggtta
    15061 ccagcataga gatactgtga ttcatcgctt gtcaggagct gggaaacttc tctttttcat
    15121 tctggtatca ttggcgacca tgattagcta tgataccaga atgctcttgc tgattgccat
    15181 cttttcagtc tttctcctct atttatcaga aattcgcttt aaagatgttt cctttgtagc
    15241 cgtttttgcg acggtatttg ccgttttaaa cgttttgatg gtctatctct tttctcccga
    15301 gtatggggtt ggactttatg gagagagaag tgtgatttgg cagggaatcg gtgtctacac
    15361 tctaaccagc caggagctct tttatctgct aaatttggcc attaagtatc tttgcaccat
    15421 tcctctggct attatctttt tgatgacaac ccatcctagt cagtttgctt ccagtttaaa
    15481 tcaaattggt gtgccctata agattgctta ttctgtcagc ctgaccttgc gctatattcc
    15541 agatttgcag gaagaattct ttactatcaa gatgtctcag gaggcgcgtg ggatggaatt
    15601 atccaagaaa gcttctctta tgcaacgaat caaaggcaat ctgctcatta ttacgccctt
    15661 gatttttagc tcgctagaac gcattgatac cattgcgact gccatggagc ttcgccgctt
    15721 tgggaaagag aaaaaacgca catggtatag ttatcaggcc ttgaaaaaag gagactatat
    15781 taccttgctc ttggcaacct tgtttttagt agttagttta ctacttatct tgcagaatca
    15841 gggacgattt tacaaccctt ggaaatagct tgaaaaaatt gaaaaaatca agtcatttct
    15901 attgacaatg attctgaaag tgttatacta agaaagtagt ttcgctgatt tacttcaaac
    15961 ctgttgtgag gtaagttaac gatgccttaa ccacgctgtt tgctgagctt gactccgggc
    16021 agtgtggcta tttttttgca atggtgaaag gaagcaagtc atgacaaatc acattgtatt
    16081 atttgaacct caaattccac aaaatacagg caatatcgcg cgtacttgcg ctgcgaccaa
    16141 ttctcccctc cacatcatca agcctatggg ctttcctatt gatgaccgca agatgaagcg
    16201 ggctggattg gattactggg ataagctaga gatttatttt tacgagagct tagaagattt
    16261 catgtctcag atgaagggaa aactctatct gatttcgaaa ttcgcggaaa aaatctattc
    16321 tgaggtggat ttatcgactg acgaggacca ttattttctc tttggacgtg aagacaaggg
    16381 cttacctgaa gattttatgc gagaacatcc tgagaaggct cttcgtattc ccatgaatga
    16441 tgaacatgtc cgcagtctca atgtgtctaa taccgtctgc atgattgtct atgaggctct
    16501 tcgccagcag aactttgcag gtcttgaact cgtccatacc tatgaagtgg ataaattgaa
    16561 ataaaaaatg aaaatgaaca aaatgcttgc gcttgcaagc gttttttgtt atgataaaag
    16621 agtcttcagg gcagggtgtg attcccgacc ggcggtgact ttaactagga aatgatcttt
    16681 tccttttata ctttgttgac aagctttgcc taaccagaag ttatgcctac agcttgtcgc
    16741 ctagtctaaa agaaaaatct ctatttcctt ctctttaaag aagtccgcga gcgcaagctg
    16801 atgtggtgag attccacaac cgacagtata gtctggatgg gagaagacga aagaatagct
    16861 ttgtctgttt tgatagattt atagccggat tgtaaccact tgctttgttt ttctttaata
    16921 gagtaagagg gaacttttgg gatataaaaa gtgagaatag atagaggaat cctttccaac
    16981 ttcttctgat tttatagaaa attggaggaa cctgttatga caaacacacg tcgactttcg
    17041 accattgcaa ttctatcagc catctcattt gtgctgatgt actttgactt tccgctttta
    17101 ccagcggcat ctttcctcaa gatcgaattt agtatcttgc cagtccttgt tggtctggtg
    17161 gtcatggatt tgcctgctgc tctaggagtg ctcttgcttc gctcactatt gaaattgctt
    17221 cttaacagcc aaggagtgaa tacttacatt ggtttgccga tgaatatcgt agctttggga
    17281 gtttttgtca tcgtatttgc tttgatttgg aaaaaggaac ggacaaccct tcgtttccta
    17341 ctaggctctc tagctggaac tgttggttta accttggcca tgttggttct caactatgtt
    17401 tatgctgttc ctttgtacgc taagtttgct aactttgata ttggaaaaat tttgggactt
    17461 tccaactacc taatgaccat ggtattacct tttaacttga ttgagggtgt aatcttttcc
    17521 gtttcattct ggttgttgta tgttctcttg aaaccaacct taaaacatta tgagagataa
    17581 acaaacattt ttaatgaagg gcagttttgc ccttttactt ttcgttattc ttggctacat
    17641 ggtcaaattt taccctgaaa cgctggtcgg ttttgaccaa tcgattcaga ctgccattcg
    17701 aggagactta ccagattatt tgactattct ttttcgatcc ctcacacgtc tgattgatat
    17761 tccagtgatt atcacttggg ttgtcattat agcttttgtc ttttatcgta agcgatggaa
    17821 gatagaaagt ttcttcatgc tgggaaatct ggctttggca ggtcttttaa tcgtgacctt
    17881 taaaaatatc taccagcgcc cacgaccagc tattttacat ctggtggagg agaagggatt
    17941 ttccttccca agtgggcatt ctctagctgt aacgctgatg gtagggactt tgattgttat
    18001 tcttagtcag cggattaaag atcaggtctg gagaaaaatc gtacaaatcg tccttggcct
    18061 ctacctagtc agtgtgctgg tatcaagagt ctatctggga gttcattacc catcagacgt
    18121 ccttgccagt ctctgtgtgg gcttgggagt cctgtttatc gagtttccct tttatgacaa
    18181 gctccgcttc caatggcgat ttaaaggcaa acagaagtga gtatcgaatt cccttgagag
    18241 aaagaaatga aagtcaatat agcagatctt catccgactc aactatattt atcagaaaag
    18301 aagttgcaag atattcagat gctttaccag tcggcagaaa caatccaagt cgatccaatc
    18361 agtattcttg cctttggaga ttgtttgctg attacagatg ggcatcacag ggcttatcag
    18421 gctttattgg caggtcggga tacgatttct gctgagtggg atagagatgg tggtgatgaa
    18481 ctatatcatt tctatgcgca agcttgcgag gaaagaaaga tttactctgt ttttgattta
    18541 gaagatcgta tcctagctca agatgggtat gaagcaaaat ggtataactg gtgtgatggt
    18601 tttaatcaag cagcaactct tttgttgaaa aggtagtgca ttgtgttttg ttccttatta
    18661 taaggtcaat cattgtgagg aagcttttgc ttggtatcag gatgtgaact tggtttacct
    18721 cgtagatggg gtgaagcttc cttacagtca agcgaccttg gaagccatgt attcctattt
    18781 ggatcggcat ggtgagcttt tttggattga agtcaaggag aagggagaat ggtttccaat
    18841 tggggatatt acactatctc aggataatct ccccattgtg attgggaatt ccgcttacca
    18901 acatcgagga cttggaaaaa gattctaagt gctttgattg aattggctcg agtaaaagga
    18961 tggaaagaat tgagagtcaa ggaaatctac acctacaatc atgcttctag gaggtgtttc
    19021 aagtcgcttg gatttgtgga aaatggagca acagaaaaag gaaggggttt tatattggaa
    19081 ttagtctaat cctgccactt tctccccctt aacctttgac tattccgcaa aattatcgta
    19141 aaataaagag taaatgataa aatgaggtca gagtctgttc gctctgacga tagtagtata
    19201 aatgaggaga aacgctttgg aattagaagt atttgctggg caagaaaaaa gtgaactatc
    19261 tatgattgag gtagcgcgtg ctatcttaga acttcgtggt cgcgatcatg agatgcattt
    19321 tagcgatctt gtaaacgaaa ttcaaaacta ccttggaaca tcaaacagcg atatccgcga
    19381 agctttgcct ttgttctaca cagagttgaa ctttgacggt agcttcatct cacttgggga
    19441 caacaaatgg ggtcttcgtt catggtatgg tgtggacgaa atcgacgaag aaatcatcgc
    19501 tcttgaagaa aatgacgacg atgaagtagc accaaaagct aagaaaaaac gtgtcaatgc
    19561 ctttatggat ggtgattcag atgccattga ctacaatgca gatgatccag aagacgaaga
    19621 tgcatacgaa gcagatccag ctctttcata cgatgatgaa aatccagatg atgaaaaaaa
    19681 tgaagtggaa gcttatgatg cagaaatcaa cgaaatcgct ccagatgact tgggagaaga
    19741 tgtggatctc aacgaagacg acgacgagtt ttcagatgac gacgctgaaa ccagcgagga
    19801 ataagagtta gctattgaca attatcctat ttttaggtat catattgttt gggcacctct
    19861 tttagaggtc ggggctccct agttcttagg gagctatttt tgttttttca agaagttatc
    19921 ttcttgtatt ttatactcaa tgaaaatcaa agagcaagct aggaaactag ccgtaggctg
    19981 ctcaaaacac tgttttgagg ttgtagataa gactgacaaa gtcaggaaca catatctacg
    20041 gcaaggcgac gttgacgcgg tttgaagaga ttttcgaaga gtattagttg tgaatctggt
    20101 gcagtcgtcc cagattattc ttattagtag ggtcttgttt tctatatccc ctcgtagtta
    20161 acaagacctt gagcatttta gaaagaggaa tctatgtcta cgaaatatat ttttgtaact
    20221 ggtggtgtgg tatcgtctat tgggaaaggg attgtggcag cgagtctagg ccgtctcttg
    20281 aaaaatcgtg gtctcaaagt aaccattcaa aagtttgacc cttatatcaa tattgatccg
    20341 ggaaccatga gtccttacca gcacggggaa gtttttgtga cagatgacgg agctgagaca
    20401 gatttggact tgggtcacta tgaacgtttc atcgatatca atctcaacaa atattccaac
    20461 gtgacaactg ggaaaattta cagtgaagtt cttcgtaaag aacgccgtgg agaatacctt
    20521 ggggcaactg ttcaagtcat tcctcatatc acagatgctt tgaaagaaaa aatcaagcgt
    20581 gccgctctaa cgaccgactc tgatgtcatt atcacagagg ttggtggaac agtaggagat
    20641 atcgagtcct tgccattcct agaggctctt cgtcagatga aggcagatgt gggtgcggat
    20701 aatgtcatgt atatccatac aaccttgctt ccttacctca aggctgctgg tgaaatgaaa
    20761 accaaaccaa cccaacactc tgtcaaagaa ttgcgtggct tgggaatcca accaaatatg
    20821 ttggttattc gtacagaaga gccagctggt caaggaatta aaaataaact ggcccagttc
    20881 tgtgatgtgg caccagaagc cgttatcgaa tcgttggatg ttgaacacct ttaccaaatt
    20941 ccactgaact tgcaggcaca agggatggac caaattgttt gtgatcattt gaaattagac
    21001 gcaccagcag cggatatgac agaatggtca gccatggtgg acaaggtcat gaacctcaag
    21061 aaacaagtta agatttccct tgttggtaag tatgtggagt tgcaagatgc ctatatctca
    21121 gtggtcgaag ctttgaaaca ctctggctat gtcaatgatg cagaagttaa aatcaattgg
    21181 gtcaatgcca atgatgtgac agcagagaat gtagcagaac tcttgtctga tgcagacggg
    21241 atcatcgtac caggtggttt tggtcaacgt ggtacagaag ggaaaatcca agccatccgc
    21301 tatgcgcgtg aaaatgatgt tccaatgttg ggagtctgct tgggaatgca gttgacatgt
    21361 atcgagtttg ctcgtcacgt tttaggtctt gaaggtgcca attctgcaga gcttgcacca
    21421 gaaacaaaat accctatcat tgatatcatg cgtgatcaga ttgatattga ggatatgggt
    21481 ggaacccttc gtttaggact ttatccgtct aagttgaaac gtggctctaa ggctgctgct
    21541 gcttatcaca atcaagaagt ggtgcaacgc cgtcaccgtc accgttatga gtttaataat
    21601 gccttccgtg agcagtttga ggcagcaggt tttgtctttt caggagtttc tccagacaat
    21661 cgtttggtag aaatcgtgga aattcctgaa aataaattct ttgtagcttg tcagtatcac
    21721 cctgaactgt caagccgtcc aaaccgacca gaagaactct acactgcctt tgttactgca
    21781 gcggttgaga acagcaatta gcaaaatcag aacctttgag aaaaatctca gaggtttttt
    21841 gcatacaatg atattgcagt atatctgagg taggagtcct ctgtatgtac ctgctaccgt
    21901 tgaaattaat agcgactccc tcttgccctg tgctagtgaa tggatttatc agtatattga
    21961 aatgaaataa aatctgaaca aattaattcg gaaagccaaa tcaatttcta gcaaagtttt
    22021 aggaactgga ttgtatagtg aa
//
LOCUS       DAVKGU010000023        21719 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKGU010000023 DAVKGU010000000
VERSION     DAVKGU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.023"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            40..1068
                     /locus_tag="VW077_000878"
     CDS             40..1068
                     /locus_tag="VW077_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU9454925.1"
                     /translation="MTKVRINWVDFGKGFAIFLVLVGHVFIGLYESNKFSIANDVLLF
                     LIAQIYIFHIPVFFALSGYFFRPVSDLKEFWHYAKKKTIILGIPYIFYSIIHFCLQKV
                     AGASVRVPTTIFNLLNIYKFPLGVSWYLYTLWSILIVYGLLSVVFKNRKSLLLVSVFA
                     YIFTLFIQTDIFIVQRTLVWGICFLLGSVLSEIHFDKINFKKILVFFVLFDFIYMFAW
                     FLFYEVESKKDYVSYSNPGLWGIAFIVCVLVAFAIFPKMEKNFPKTFLYFTKYGKDSL
                     GIYILHAPICSMIRILMLKVGINSVFLHVVVGIVLGWYLSILATYILKKIPFLNIVLL
                     PQKYIKLK"
     gene            1246..3228
                     /locus_tag="VW077_000879"
     CDS             1246..3228
                     /locus_tag="VW077_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9454926.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVQFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            3529..8832
                     /locus_tag="VW077_000880"
     CDS             3529..8832
                     /locus_tag="VW077_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9454927.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTKNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDNERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDESKLLSSISF
                     LGNALVSVSSNQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREASDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FESLDAPAQPGEGLENAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFAATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKSDAPTVEKPEFKLSSLASEQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(8999..11158)
                     /locus_tag="VW077_000881"
     CDS             complement(8999..11158)
                     /locus_tag="VW077_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU9454928.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     SIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEQETGYNLLTTGMDVYTNVDQEAQKHLWDIYNSDQYVSYPDDDLQVASTVV
                     DVSNGKVIAQLGARHQASNVSFGTNQAVETNRDWGSAMKPITDYAPAIEYGVYDSTAT
                     MVNDIPYNYPGTSTPVYNWDRAYFGNITLQYALQQSRNVTAVETLNKVGLDRAKTFLN
                     GLGIDYPSMHYANAISSNTTESNKQYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSKKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRGAYLPWLPQAGKTGTSNYTDE
                     EVENHIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFLVAAKVYRSMITY
                     LSEDTHPEDWTMPDGLFRNGEFVFKNGARPIWTEPSTQQSSTAESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(11155..11751)
                     /gene="recU"
                     /locus_tag="VW077_000882"
     CDS             complement(11155..11751)
                     /gene="recU"
                     /locus_tag="VW077_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9454929.1"
                     /translation="MVNYPHKLSSQKRQPSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVRVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEAKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLGYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            11818..12345
                     /locus_tag="VW077_000883"
     CDS             11818..12345
                     /locus_tag="VW077_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9454930.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            12415..12756
                     /gene="gpsB"
                     /locus_tag="VW077_000884"
     CDS             12415..12756
                     /gene="gpsB"
                     /locus_tag="VW077_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9454931.1"
                     /translation="MTSIIFSAKDIFEQEFGREVRGYSKVEVDEFLDDVIKDYETYAT
                     LVKSLRQEIADLKEELTRKPQVSSAPSPSHPDPIDVAASSSMTNFDILKRLNRLEKEV
                     FGKQILDNTDL"
     gene            12777..13157
                     /gene="rnpB"
                     /locus_tag="VW077_000885"
     ncRNA           12777..13157
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW077_000885"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13242..14399
                     /locus_tag="VW077_000886"
     CDS             13242..14399
                     /locus_tag="VW077_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9454932.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     TIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIESVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            14412..15806
                     /gene="mapZ"
                     /locus_tag="VW077_000887"
     CDS             14412..15806
                     /gene="mapZ"
                     /locus_tag="VW077_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9454933.1"
                     /translation="MSKKRRNRHKKEAQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            15882..17306
                     /gene="gndA"
                     /locus_tag="VW077_000888"
     CDS             15882..17306
                     /gene="gndA"
                     /locus_tag="VW077_000888"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9454934.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            17318..18007
                     /locus_tag="VW077_000889"
     CDS             17318..18007
                     /locus_tag="VW077_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9454935.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            18106..19104
                     /gene="cbpJ"
                     /locus_tag="VW077_000890"
     CDS             18106..19104
                     /gene="cbpJ"
                     /locus_tag="VW077_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9454936.1"
                     /translation="MKLLKKMMPIALAVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNNWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQYLGNKWYYLRSSGAMVTGW
                     YQDGLTWYYLNAGNGDMKTGWFQVNGKWYYAYSSGALAVNTTVDGYYVNYNGEWVR"
     gene            complement(19222..20454)
                     /locus_tag="VW077_000891"
     CDS             complement(19222..20454)
                     /locus_tag="VW077_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9454937.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLAMLSIIIS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(20613..21083)
                     /locus_tag="VW077_000892"
     CDS             complement(20613..21083)
                     /locus_tag="VW077_000892"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454938.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKSNR"
     gene            complement(20983..21341)
                     /locus_tag="VW077_000893"
                     /pseudo
     CDS             complement(20983..21341)
                     /locus_tag="VW077_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
BASE COUNT         6746 a         3860 c         4813 g         6300 t
ORIGIN      
        1 agaattctta atattatatt gtaatggagg cattttgaat tgactaaagt aagaattaat
       61 tgggtagatt ttgggaaagg atttgcaata ttcttagttc tagtagggca tgtatttatt
      121 ggtttatatg aatccaataa gttttcaatt gcaaatgatg tgttattatt cctgatagct
      181 caaatctata tatttcatat accagtgttt tttgcgttat caggatactt tttcagacct
      241 gtatcagatt tgaaggagtt ttggcattat gctaaaaaga aaaccattat tttaggcata
      301 ccatatattt tttattcaat tattcacttt tgtcttcaaa aggttgcggg tgcttctgtc
      361 cgagtaccca ctacgatatt taacctattg aatatctata aatttcctct gggagtttca
      421 tggtatctat atacgttatg gtcgatattg atagtttatg gtttactatc tgttgttttc
      481 aaaaatcgta agtccctttt gttagttagt gtttttgcct atattttcac actatttatt
      541 caaactgata tttttattgt gcaacggacg ttggtttggg ggatttgttt ccttcttggc
      601 agtgtattga gtgaaattca ctttgataaa attaatttta aaaaaattct tgttttcttt
      661 gtgttatttg attttattta tatgttcgct tggttcttgt tttatgaagt agaatctaag
      721 aaggattatg taagctatag taacccaggt ttgtggggga ttgcttttat tgtctgtgta
      781 ttagttgctt ttgcgatttt tcctaaaatg gagaaaaatt ttcctaaaac tttcctatat
      841 ttcactaaat acgggaaaga tagtttaggg atctatattc ttcatgcacc aatttgtagc
      901 atgattcgga ttctaatgtt gaaagtggga ataaactcag tttttcttca cgttgttgtt
      961 gggattgtgc taggctggta tttatccata ctggcaactt atatattgaa aaaaattcca
     1021 tttttgaata ttgttttatt accacaaaag tatattaaat taaaataaat tattttagat
     1081 ttataatgtg gctctttgtc aactaacatc tggagaggac aatcactgtc ttctcttttt
     1141 ttgtcttttc agaatatacc aaattaacac aaaaattctg aaaattctgt tgacatcttt
     1201 ctgaaaagag tctataatgg agagaaagct ttaaaggaga aaatgatgaa aagttcaaaa
     1261 ctatttgccc ttgcgggcgt gacattattg gcggcgacta ctttagctgc atgctctgga
     1321 tcaggttcaa gcactaaagg tgagaagaca ttctcataca tttatgagac agaccctgat
     1381 aacctcaact atttgacaac tgctaaggct gcgacagcaa atattacaag taacgtggtt
     1441 gatggtttgc tagaaaatga tcgctacggg aactttgtgc cgtctatggc tgaggattgg
     1501 tctgtatcca aggatggatt gacttacact tatactatcc gtaaggatgc aaaatggtat
     1561 acttctgaag gtgaagaata cgcggcagtc aaagctcaag actttgtaac aggactaaaa
     1621 tatgctgctg ataaaaaatc agatgctctt taccttgttc aagaatcaat caaagggttg
     1681 gatgcctatg taaaagggga aatcaaagat ttctcacaag taggaattaa ggctctggat
     1741 gaacagacag ttcagtacac tttgaacaaa ccagaaagct tctggaattc taagacaacc
     1801 atgggtgtgc ttgcgccagt taatgaagag tttttgaatt caaaaggaga tgattttgcc
     1861 aaagctacgg atccaagtag tctcttgtat aacggtcctt atttgttgaa atccattgtg
     1921 accaaatcct ctgttgaatt tgcgaaaaat ccgaactact gggataagga caatgtgcat
     1981 gttgacaaag ttaaattgtc attctgggat ggtcaagata ccagcaaacc tgcagaaaac
     2041 tttaaagatg gtagccttac agcagctcgt ctctatccaa caagtgcaag tttcgcagag
     2101 cttgagaaga gtatgaagga caatattgtc tatactcaac aagactctat tacgtatcta
     2161 gttggtacaa atattgaccg tcagtcctat aaatacacat ctaagaccag cgacgaacaa
     2221 aaggcatcga ctaaaaaggc tctcttaaac aaggatttcc gtcaggctat tgcctttgga
     2281 tttgaccgta cagcctatgc ctctcagttg aatggacaaa ctggagcaag taaaatcttg
     2341 cgtaatctct ttgtgccacc aacatttgtt caagcagatg gtaaaaactt tggcgatatg
     2401 gtcaaagaga aattggtcac ttatggggat gaatggaagg atgttaatct tgcagattct
     2461 caggatggtc tttacaatcc agaaaaagcc aaggctgaat ttgctaaagc taaatcagcc
     2521 ttacaagcag aaggagtcca attcccaatt catttggata tgccagttga ccagacagca
     2581 actacaaaag ttcagcgcgt ccaatctatg aaacaatcct tggaagcaac tttaggagct
     2641 gataatgtca ttattgatat tcaacaacta caaaaagacg aagtaaacaa tattacatat
     2701 tttgctgaaa atgctgctgg cgaagactgg gatttatcag ataatgtcgg ttggggtcca
     2761 gactttgccg atccatcaac ctaccttgat attatcaaac cttctgtagg agaaagtact
     2821 aaaacatatt tagggtttga ctcaggggaa gataatgtag ctgctaaaaa agtaggtcta
     2881 tatgactacg aaaaattggt tactgaggct ggtgatgaga ctacagatgt tgctaaacgc
     2941 tatgataaat acgctgcagc ccaagcttgg ttgacagata gtgctttgat tattccaact
     3001 acatctcgta cagggcgtcc aatcttgtct aagatggtac catttacaat accatttgca
     3061 ttgtcaggaa ataaaggtac aagtgaacca gtcttgtata aatacttgga acttcaagac
     3121 aaggcagtca ctgtagatga ataccaaaaa gctcaggaaa aatggatgaa agaaaaagaa
     3181 gagtctaata aaaaggctca agaagatctc gcaaaacatg tgaaataact gttgcaaaat
     3241 ataagaaagg atttagtatt tctcttgaat gctgaatcct tttttacatt tgtaaagaaa
     3301 gattctaaat gtactgaccc tcaaaagttg gagcctcttt ttgtcagaat agagaaaatt
     3361 tttgttaatt ttacttgttt cctattgctt tctcagctac tatttgttat attaaaagta
     3421 taattatttt ttatttatca gggttaagca ttgcactttc agaggaagga gtatttttta
     3481 aaaaggaaat gtaaacgctt gctcaaaaat gaaaggattt agaagtttat gaataaagga
     3541 ttatttgaaa aacgttgtaa atatagtatt cggaaatttt cattaggtgt tgcttctgtt
     3601 atgattggag ctgcattctt tgggacaagt ccggttcttg cagatagcgt gcagtctggt
     3661 tccacggcga atttaccagc tgatttagct actgctcttg caacagcaaa agagaatgat
     3721 gggcgtgatt ttgaagcgcc taaggtggga gaagaccaag gttctccaga agttacagat
     3781 ggacctaaga cagaagaaga actattagca cttgaaaaag aaaaaccggc tgaagaaaaa
     3841 ccaaaagagg ataaacctgc agctgctaaa cctgaaacac ctaagacggt aacccctgaa
     3901 tggcaaacgg tagagaaaaa agaacaacag ggaacagtca ctatccgaga agaaaaaggt
     3961 gtccgctaca accaattatc ctcaactgct caaaatgata acgcaggtaa accagccctg
     4021 tttgaaaaga agggcttgac cgttgatgcc aatggaaatg caactgttga tttaaccttc
     4081 aaagatgatt ctgaaaaggg caaatcacgc tttggtgtct tcttgaaatt taaagatacc
     4141 aagaataatg tttttgtcgg ttatgacaag gatggctggt tctgggagta taaatctcca
     4201 acaactagca cttggtatag aggtagtcgt gttgctgctc ctgaaacagg atcaacaaac
     4261 cgtctctcta tcactctcaa gtcagacggt cagctaaatg ccagcaataa tgatgtcaat
     4321 ctctttgaca cagtgactct accagctgcg gtcaatgacc atcttaaaaa tgagaagaag
     4381 attcttctca aggcgggctc ttatgacaat gagcgaacag ttgttagcgt taaaacggat
     4441 aaccaagagg gggtaaaaac agaggatacc cctgctgaaa aagaaacagg tcctgaagtt
     4501 gatgatagca aggtgactta tgacacgatt cagtctaagg tcctcaaagc agtgattgac
     4561 caagccttcc ctcgtgtcaa ggaatacagc ttgaacgggc atactttgcc aggacaggtg
     4621 caacagttca accaagtctt tatcaataac caccgaatca cccctgaagt cacttataag
     4681 aaaatcaatg agacaacagc agagtacttg atgaagcttc gcgatgatgc tcacttaatc
     4741 aatgcggaaa tgacagtacg cttgcaagtt gtagacaatc aattgcactt tgatgtgact
     4801 aagattgtca accacaatca agtcactcca ggtcaaaaga ttgatgacga aagcaaacta
     4861 ctttcttcta ttagtttcct cggcaatgct ttagtctctg tttctagtaa tcaaactggt
     4921 gctaagtttg atggggcaac catgtcaaac aatacgcatg tcagcggaga tgatcatatc
     4981 gatgtaacca atccaatgaa ggatttggct aagggttaca tgtatggatt tgtttctaca
     5041 gataagcttg ctgctggtgt ttggagtaac tctcaaaaca gctatggtgg tggttcgaat
     5101 gactggactc gtttgacagc ttataaagaa acagtcggaa atgccaacta tgtaggaatc
     5161 cacagctctg aatggcaatg ggaaaaagct tataagggta ttgttttccc agaatacacg
     5221 aaggaacttc caagtgctaa ggttgttatc actgaagatg ccaatgcaga caagaaagtt
     5281 gattggcaag atggtgccat tgcttatcgt agcattatga acaaccctca aggttgggaa
     5341 aaagttaagg atatcacagc ttaccgtatc gcgatgaact ttggttctca agcacaaaac
     5401 ccattcctta tgaccttgga tggtatcaag aaaatcaatc tccacacaga tggtcttggg
     5461 caaggtgttc tccttaaagg atatggtagc gaaggtcatg actctggtca cttgaactat
     5521 gctgatattg gtaagcgtat cggtggtgtc gaagacttca agaccctaat tgagaaggct
     5581 aagaaatatg gagctcatct aggtatccac gttaacgctt cagaaactta tcctgagtct
     5641 aaatacttca atgaaaaaat tctccgtaag aatccagatg gaagctatag ctatggttgg
     5701 aactggctag atcaaggtat caacattgat gctgcctatg acctagctca tggtcgtttg
     5761 gcacgttggg aagatttgaa gaaaaaactt ggtgacggtc tcgactttat ctatgtggac
     5821 gtttggggta atggtcaatc aggtgataac ggtgcctggg ctacccacgt tcttgctaaa
     5881 gaaattaaca aacaaggctg gcgctttgcg atcgagtggg gccatggtgg tgagtacgac
     5941 tctaccttcc atcactgggc agctgacttg acctacggtg gctacaccaa taaaggtatc
     6001 aacagtgcca tcacccgctt tatccgtaac caccaaaaag atgcttgggt tggggactac
     6061 agaagttatg gtggtgcagc caactatcca ctgctaggtg gctacagcat gaaagacttt
     6121 gaaggctggc aaggaagaag tgactacaat ggctatgtaa ccaacttatt tgcccatgac
     6181 gtcatgacta agtacttcca acacttcact gtaagtaaat gggaaaatgg tacaccggtg
     6241 actatgaccg ataacggtag cacctataaa tggactccag aaatgcgagt ggaattggta
     6301 gatgctgaca ataataaagt agttgtaact cgtaagtcaa atgatgtcaa tagcccacaa
     6361 tatcgcgaac gtacagtaac tctcaacgga cgtgtcatcc aagatggttc agcttacttg
     6421 actccttgga actgggatgc aaatggtaag aaactttcta ctgataagga aaagatgtac
     6481 tacttcaata cgcaggccgg tgcaacaact tggacccttc caagcgattg ggcaaagagc
     6541 aaggtttacc tttacaagct aactgaccaa ggtaagacag aagagcaaga actaactgta
     6601 aaagatggta aaattaccct agatcttcta gcaaatcaac catatgttct ctatcgttcg
     6661 aaacaaacta atcctgaaat gtcatggagt gaaggcatgc acatctatga ccaaggattt
     6721 aatagcggta ccttgaaaca ttggaccatt tcaggcgatg cttctaaggc agaaattgtc
     6781 aagtctcaag gggcaaacga tatgcttcgt attcaaggaa acaaagaaaa agttagtctc
     6841 actcagaaat taactggctt gaaaccaaat accaagtatg ccgtttatgt tggtgtagat
     6901 aaccgtagta atgccaaggc aagtatcact gtgaatactg gtgaaaaaga agtgactact
     6961 tataccaata agtctctcgc gctcaactat gttaaggcct acgcccacaa tacacgtcgt
     7021 gacaatgcta cagttgacga tacaagttac ttccaaaaca tgtacgcctt ctttacaact
     7081 ggatcggacg tctcaaatgt tactctgaca ttgagtcgtg aagctagtga tcaagcaact
     7141 tactttgatg aaattcgtac ctttgaaaac aattcaagca tgtacggaga caagcatgat
     7201 acaggtaaag gcaccttcaa gcaagacttt gaaaatgttg ctcagggtat cttcccattt
     7261 gtagtgggtg gtgtcgaagg tgttgaagat aaccgcactc acttgtctga aaaacacgat
     7321 ccatatacac aacgtggttg gaatggtaag aaagtcgatg atgttatcga aggaaattgg
     7381 tcactcaaga caaatggact agtgagccgt cgtaacttgg tttaccaaac tattccgcaa
     7441 aacttccgtt ttgaagcagg taagacctac cgtgtaacct ttgaatacga agcaggttca
     7501 gacaatacat atgcttttgt agtcggtaag ggagaattcc agtcaggtcg tcgtggtact
     7561 caagcaagca acttggaaat gcatgaattg ccaaatactt ggacagattc taagaaagcc
     7621 aagaaggcaa ccttcctcgt gacaggtgca gaaacagggg atacttgggt aggtatctac
     7681 tcaactggaa atgcaagtaa tactcgtggt gattctggtg gaaatgccaa cttccgtggt
     7741 tataacgact tcatgatgga taatcttcaa atcgaagaaa ttaccctaac aggtaagatg
     7801 ttgacagaaa atgctctgaa gaactacttg ccaacggttg ccatgactaa ctacaccaaa
     7861 gagtctatgg atgctttgaa agaggcggtc tttaacctca gtcaggccga tgatgatatc
     7921 agtgtggaag aagcgcgtgc agagattgcc aagattgaag ccttgaagaa tgctttggtt
     7981 cagaagaaaa cggctttggt agcagaagac tttgaaagtt tggatgcgcc agctcaacca
     8041 ggtgaaggcc tagagaatgc ttttgatggc aatgtgtcta gtctatggca tacatcttgg
     8101 aatggtggag atgtaggcaa gcctgcaacc atggtcttga aagaaccaac tgaaatcact
     8161 ggacttcgtt acgttccacg tggatcgggt tcaaatggta acttgcgtga tgtgaaactt
     8221 gttgtgacag atgagtctgg caaggagcat acctttgctg caactgattg gccagataac
     8281 aataagccaa aagacattga ttttggtaag acaattaagg ctaagaaaat tgtccttaca
     8341 ggtactaaga cttacggaga tggtggcgat aaataccaat ctgcagcgga acttatcttt
     8401 actcgtccac aggtagcaga aacacctctt gacttgtcag gctatgaagc agctttggct
     8461 aaggctcaga aattaacaga caaagacaat caagaggaag tagctagcgt tcaggcaagc
     8521 atgaaatatg cgacggataa ccatctcttg acggaaagaa tggtggaata ctttgcagat
     8581 tatctcaacc aattaaaaga ttctgctacg aaatcagatg ctccaactgt agagaaacct
     8641 gagtttaaac ttagctcttt agcttccgaa caaggtaaga cgccagatta taagcaagaa
     8701 atagatagac cagaaacacc tgaacaaatc ttgccagcaa caggtgagag tcaatctgac
     8761 acagccctct tcctagcagg tgttagccta gccctatctg ctctctttgt agtaaaaacg
     8821 aagaaagact agtatttagt aaaacctctt aacaagatta cggaagcagt ctctatcttt
     8881 tccaatgagg tttatagtac agaaaaagcc tgagaagatg tcttctcagg cttttgttaa
     8941 gcacataaat acaatagtgc tatgaaaaaa tcacccagaa aaatctggat gataaatgtt
     9001 atggttgtgc tggttgagga ttctgatttt gttgatcagg ggttgtattt gattgttgcg
     9061 tattattgtt aggattggta gtcgtactat tatttgtgct tggagtggtt gagctagact
     9121 gtgaagttga actatctgat gatgagcttg aactttcagc tgttgaggat tgttgagtag
     9181 agggttcagt ccatattggg cgagctccat ttttgaatac aaattccccg tttctgaaaa
     9241 gtccgtctgg catcgtccag tcttctggat gagtatcttc tgatagatac gttatcattg
     9301 agcgataaac tttagctgca actaggaaac catctccaac gataggagtt aaacgattcg
     9361 aataacctgt ccatacagcc atagaatact tacgagtata accaacaaac atttcatctg
     9421 gagctacata gccagtgttc ttgatgtggt tttcaacttc ctcatctgta tagttagagg
     9481 ttcctgtttt accagcttga ggaagccaag gaagataggc tccacgccca gttccataag
     9541 tcaagactgt tttcatcatg tcggtcatca tataggctgt cgtttccttc atggcacgag
     9601 ttccgacatt agagaactct tttttacttc catcactgaa gacgacttta tggatataca
     9661 ttggtttata gtaagtgcca ccatttgcaa aggcagcata agcagcagcc attttttcac
     9721 tacttgctcc gtattgttta ttagattctg ttgtattact tgaaatggcg tttgcataat
     9781 gcatgcttgg atagtcgata ccaagaccat taaggaaggt tttagctcta tctagaccga
     9841 ccttattcaa agtctcaacg gctgtgacat ttcgtgattg ttgaagagca tattgcagag
     9901 taatattacc gaaatatgct ctatcccagt tgtagacagg tgtgcttgtt cccggatagt
     9961 tataaggaat atcattaacc atagttgcag tggaatcata aacaccgtat tctatggcag
    10021 gtgcataatc ggtgattggt ttcatagcag aaccccagtc acgattggtt tccacagctt
    10081 ggttggtacc aaatgaaacg ttacttgctt ggtgacgagc tccaagttgg gcgatgactt
    10141 taccatttga aacatctacg accgtagatg cgacttgcaa atcatcgtca gggtaagaga
    10201 cgtattgatc ggagttgtag atatcccaca gatgtttttg agcttcttgg tctacatttg
    10261 tgtaaacatc catcccagta gttagaaggt tatagccagt ttcttgttct acttgattga
    10321 tgacctcctt gaggtaatta tccatgtaag cagggtaatt acttgctgat ttgagacttt
    10381 gtagtccatc agtaattggt gtattgactg ctttctcata ctgttcagca gagatgtaac
    10441 cttgattttt catttcagat aagaccaagt ttcggcggtc ttgggctgct tctggatgtg
    10501 aataggggtc atattggttt ggtgcctgag gcattccagc cagcaaggct aactgaggta
    10561 aacttaaatt attgaggtct ttaccatagt agttttgagc tgctgtctgc attccatagt
    10621 tgccattaga catgtagacc ttatttatat agtaggtcaa gatttcctgt ttggttgctt
    10681 tttgttctaa ctgaatcgct aaccaagctt cctgagcctt acgagaaata gtctggtcgg
    10741 aagtcgaggt tgaaaagtaa gtcaacttaa tcaactgttg ggtgagagtt gatccacctt
    10801 ggagggaatt actttgcaga ttgcgcaaga aagctcccag gatacggatg gaatcaatcc
    10861 ccctgtggtc gaagaagcga tggtcttcga tagaaacgat tgccttaacc aaatctgtgg
    10921 gaatatcatt agcttgggca ttgacgcggc gttcagaacc caagtcagca atgagttgat
    10981 ttttattgtc gtagatttta ctagaagttg ttgcaactag tttactctcg gataggctag
    11041 gagccttgct aacgtagtag aaaaaaactc ctccgcctaa gacaatggct gcgataacca
    11101 agcttaagaa gctaatgctc agatacttga ttaggcgcag aatcgttggt ttgttcatct
    11161 tgttttacca cctaataaat gttctttgat aacattgaga taaggaattt gagggaaggc
    11221 accagccttg atttcatatc catattctcg aatatatcca agtggcattg atttttgtcc
    11281 cttatcttga tgatagaagc gaatcaaatc gaatgccggc aataagtagg tttcttgctg
    11341 agaagaaaag tgaagaagga caaagcagat tccttgttgg gcaaggactt gttccatatg
    11401 ctgaatctga tgtggatgaa aatttttcat cggaatcgca cgtttttgtt ttgtttcctt
    11461 ggcttcaaag tcgatgtaat atccattata aacgccagaa tagtccgtcg ttgaagcttg
    11521 tcgaaaatag gcttcaacaa tcttggcacg acttcgttgt ggatagtcca ctcgtacgat
    11581 ttgaatagga gttggtttct tgtgtataac agccaagccc tgagacaaat agtagtcgtt
    11641 ggtagcattg atcatctttt caaaagacat tcctcgattt gcgaaatttt tgggttgaga
    11701 aagagatggt tgtctttttt gtgatgaaag tttatgtgga tagttgacca taattctcct
    11761 tattggtaca ataacatcac tctattatac cataaatttg cacagaaagg gttaaaaatg
    11821 gctacagctt tggttttggg ctattctgct tttgatttgg gtttgttttc tgataaggat
    11881 cctcgtctta aattgataaa aaaagcaata cgtaaagatt tagaggctat ggcagcagat
    11941 ggggtgtctt ggcttgtgtt tacaggaagt ttgggttttg aatattgggt tttagaggtt
    12001 gctcaggaga tgaaaaccga gtacggtttc cagttggcca ccatttttgc ttttgaaacc
    12061 catggggaaa attggaatga aggcaatcag atgaaactga gtcgttttaa gcaggtagac
    12121 tttgtcaaat atgcttatcc tcgctatgag cacaaggggc agttaagaga ttaccagcag
    12181 tttttgctgg aaaacacgac tagttcctat cttttttatg atgaagaaaa tgaaacgaaa
    12241 ctagcttatt tttaccaaaa gatgaaaaat caagaggact attttataaa gagattaaca
    12301 tttgatcaat taaatgaact tgctgaaaat ttttccgaaa attgaagctt tgaccttgat
    12361 ttttgtttgc ctttttttat ataataatac tagcaagcga gaatggagag agacatgaca
    12421 agtattattt tttcagcaaa agatattttt gaacaagagt ttggacgtga agttcgtggg
    12481 tatagcaagg tagaagttga tgagttttta gacgatgtca tcaaggacta tgaaacctat
    12541 gctaccttgg ttaagtcact tcgtcaggag attgcggatt tgaaggaaga attaactcgt
    12601 aaaccgcaag tgagttcggc tccaagtcct agtcacccag atccaattga tgtggctgct
    12661 tcatcttcta tgacgaattt tgatattttg aaacgcttga atcgtcttga aaaagaagta
    12721 ttcggtaaac aaattttaga caatactgat ttgtaattca ggtcttgtta catgcaattt
    12781 ttggataatc gcgtgaggag aattgcttct catgaggaaa gtccatgcta gcacaggctg
    12841 tgatgcctgt agtgtttgtg ctaggcgaaa ccataagcct agggacgaga aatcgttacg
    12901 gcagttgaaa tggctaagtc cttggatagg ccagagtagg cttgaaagtg ccacagtgac
    12961 ggagtctttc tggaaacaga gagagtggaa cgcggtaaac ccctcaagct agcaacccaa
    13021 attttggtcg gggcatggag tacgcggaaa cgaacgtagt attctgactg ctatcagcta
    13081 gagctgctag tggtagacag atgattatcg aaggaagtgg tcctagtcac ttctggaaca
    13141 aaacatggct tatagaaaat tgcatatagg ttggggctga gaaattttct caacctcatt
    13201 ttttaaagtg gacatataga aaggtcttgc aagactgtaa catgaaaaaa gaatttaatt
    13261 taattgcaac tgtggcagca ggtcttgaag ctgttgtggg acgagaagtg cgagagttgg
    13321 gctacgattg tcaggttgaa aatggtcgtg ttcgttttca aggagacgtg agaactatta
    13381 tcgaaaccaa cctgtggctt cgggcagcag atcgtatcaa gattatcgta ggaacgttcc
    13441 cagctaagac ttttgaagag ctgtttcagg gagttttcgc tttagattgg gaaaattatt
    13501 taccacttgg agctcggttc ccgatttcaa aagctaaatg tgttaagtcc aaacttcaca
    13561 atgagcctag tgttcaggct atttctaaga aagctgttgt caagaaattg cagaaacact
    13621 atgctcgccc agaaggggtt cctctgatgg agaatggccc agagtttaag attgaggtct
    13681 ctattctcaa agatgtggca actgtcatga ttgatacgac cgggtctagc ctctttaaac
    13741 gtggttatcg taccgaaaaa ggtggcgctc ctatcaagga aaatatggca gcagccattt
    13801 tacaactttc taactggtac ccagacaagc ctttgattga tccgacctgt ggttcgggga
    13861 ctttctgtat tgagtcagtt atgattgcta gaaagatggc gccaggtctt cgtcgctctt
    13921 ttgcatttga ggaatggaac tggatcagcg atcgcttgat tcaagaagtg cgcacagaag
    13981 cggctaaaaa agtagaacgt gagcttgagc tggatatcat gggctgtgat attgatgctc
    14041 gcatggtgga aattgctaag gccaatgctc aggcagctgg tgttgcagga gacattactt
    14101 ttaagcagat gcgcgtgcag gatttacgtt ccgataaaat caatggagta atcatttcca
    14161 atccgcctta tggtgaacgt ttgtcagatg atgcaggggt gaccaagctc tatgctgaga
    14221 tggggcaagt atttgcaccg ctgaaaactt ggagcaaatt tatcctgact agtgatgaag
    14281 cttttgaaag caagtatggt agccaagcag ataagaagcg taagttatac aacggaacct
    14341 tgaaagtgga tttatatcaa tattttggtc agcgtgtcaa acggcaagag gtaaaataga
    14401 aagggatact catgagtaaa aaaagacgaa atcgtcataa aaaagaagct caagaaccgc
    14461 aatttgattt tgatgaagca aaagagctaa cagttggtca agctattcgt aaaaatgaag
    14521 aagtggaagc aggagtcttg cctgaggatt ccattttgga caagtatgtt aagcaacaca
    14581 gagatgaaat tgaggcggat aagtttgcga ctcgtcaata caaaaaagag gagttcgttg
    14641 aaactcagag tctggatgat ttaattcaag agatgcgtga ggctgtagag aagtcagaag
    14701 cttcttcgga ggaagttcca tcttctgaag acatcttact acccttgcct ctggacgatg
    14761 aggagcaagg cttggatcct ctattgctag atgatgaaaa tccaacagaa atgactgaag
    14821 aagtggaaga ggagcaaaac ctttctcgtc tggatcaaga ggactcagaa aagaaaagta
    14881 aaaaaggctt tattttgacc gttttggcgc ttgtatcagt aattatttgt gtcagtgctt
    14941 attatgtcta tcgtcaagtg gctcgttcga ccaaggaaat tgaaacttct caatcaacta
    15001 cagccaatca gtcggatgtg gatgatttta atacacttta tgacgccttt tacacaaata
    15061 gcaataaaac ggctttgaaa aatagccagt ttgataaact gagtcaactc aagactttac
    15121 ttgataagct ggaaggtagt cgtgaacata cgcttgccaa atctaaatat gatagtctag
    15181 caacgcaaat caaggctatt caagatgtca atgcacaatt tgaaaaacca gctattgtgg
    15241 atggtgtgtt ggataccaat gccaaagcca aatcggatgc taaatttacg gatattaaaa
    15301 ctggaaatac ggagcttgat aaagtgctag ataaggctat cagtcttggt aagagccagc
    15361 aaacaagtac ttctagctca agttcaagtc aaactagcag ctcaagttca agtcaagcaa
    15421 gttcaaatac gactagtgag ccaaaaccaa gtagttcaaa tgagactaga agtagtcgca
    15481 gtgaagtcaa tatgggtctc tcgagtgcag gggttgctgt tcaaagaagt gccagtcgtg
    15541 ttgcctataa tcagtctgct attgatgata gtaataactc tgcctgggat tttgcggatg
    15601 gtgttttgga acaaattcta gcaacttcac gttcacgtgg ctatatcact ggagaccaat
    15661 atatccttga acgtgtcaat atcgttaacg gcaatggtta ttacaacctc tataagccag
    15721 atggaaccta tctctttacc cttaactgta agacaggcta ctttgtcgga aatggcgctg
    15781 gtcatgcgga tgacttggac tactaagcag tcgttacaaa attctttctt ttcaaaagta
    15841 aaaatgataa aataaaacaa atgaaacaag aggagtgtca aatgacaaaa gctaactttg
    15901 gtgtcgtagg tatggccgta atgggtcgta accttgccct taatattgaa tcacgtggtt
    15961 acacagttgc tatctacaac cgtagtaaag aaaaaacgga agatgtgatt gcttgccatc
    16021 ctgaaaagaa ttttgtacca agctatgacg ttgaaagttt tgtaaactca atcgaaaaac
    16081 ctcgtcgtat catgctgatg gttcaagctg gacctggtac agatgctact atccaagccc
    16141 ttcttccaca ccttgacaaa ggtgatatct tgattgacgg aggaaacact ttctacaaag
    16201 ataccatccg tcgtaatgaa gaattggcaa actcaggtat caactttatc ggtactggag
    16261 tttctggtgg tgaaaaaggt gcccttgaag gtccttctat catgcctggt ggacaaaaag
    16321 aggcctacga attggtttcg gatgttcttg aagaaatctc agctaaagca ccagaagatg
    16381 gcaaaccatg tgtgacttat atcggtcctg atggagctgg ccactatgtg aaaatggttc
    16441 acaacggtat cgagtacggt gatatgcaat tgatcgcaga aagctatgac ttgatgcaac
    16501 acttgctagg cctttctgca gaggatatgg ctgaaatctt tactgagtgg aacaagggtg
    16561 aattggatag ctacttgatc gaaatcacag ctgatatctt gagccgtaaa gacgatgaag
    16621 accaagatgg accaatcgta gactacatcc ttgatgctgc aggtaacaag ggaactggta
    16681 aatggacgag ccaatcatct cttgaccttg gtgtaccatt gtcactgatt actgagtcag
    16741 tgtttgcacg ctacatttca acttacaaag aagaacgtgt acatgctagc aaggtgcttc
    16801 caaaaccagc tgccttcaac tttgaaggag acaaggctga attgattgaa aaaatccgtc
    16861 aagcccttta cttctcaaaa atcatttcat acgcacaagg atttgctcaa ttgcgtgtag
    16921 cctctaaaga aaacaactgg aacttgccat ttgcagatat cgcatctatc tggcgtgatg
    16981 gctgtatcat ccgttctcgt ttcttgcaaa agattacaga tgcttacaac cgcgatgcag
    17041 atcttgccaa ccttcttttg gacgagtact tcttggatgt tactgctaag tatcaacaag
    17101 cagtgcgtga tatcgtagct cttgcggttc aagctggtgt gccagtgcca actttctcag
    17161 cagctattac ttactttgat agctaccgtt cagctgacct tccggctaac ttgatccaag
    17221 cacaacgtga ctactttggt gctcacactt accaacgtaa agacaaagaa ggaaccttcc
    17281 actactcttg gtatgacgaa aaataagtag gtcagccatg gggaaacgga ttttattact
    17341 tgagaaagaa cgaaatctag ctcatttttt aagtttggaa ctccagaaag agcagtatcg
    17401 ggttgatctg gtagaggagg ggcaaaaagc cctttccatg gctcttcaga cagactatga
    17461 tttgatttta ttgaacgtta atctgggaga tatgatggct caggattttg cagaaaaatt
    17521 gagtcgaact aaacctgcct cagtcatcat gattttagat cattgggaag acttgcaaga
    17581 agagctggaa gttgttcagc gttttgcagt ttcatacatc tataagccag tctttatcga
    17641 aaatctggta gcgcgtattt cggcaatctt ccgaggtcgg gacttcattg atcaacactg
    17701 cagtctgatg aaagttccaa ggacctaccg caatcttagg atagatgttg aacatcacac
    17761 ggtttatcgt ggtgaagaga tgattgctct gacccgccgt gagtatgacc ttttggcgac
    17821 acttatggga agcaagaaag tattgactcg tgagcaattg ttggaaagtg tttggaagta
    17881 tgaaagtgcg accgagacaa atatcgtaga tgtctatatc cgctatctac ggagcaagct
    17941 tgatgttaaa ggccaaaaaa gctacattaa aaccgtgcgt ggtgttggtt acaccatgca
    18001 agaatagaaa agcagttgca gttgtgtaac tgcttttttg aggagttttt atatattgac
    18061 atacaatcta gtttttacta caatcgtttt aggaggatat ataaaatgaa gcttttgaaa
    18121 aaaatgatgc caatcgcatt agcagtcttt ttctttggtt tgctagggac cagtacagta
    18181 tttgcagatg attctgaagg atggcagttt gtccaagaaa acggaagaac ctactacaaa
    18241 aagggggacc tcaaagaaac ctactggcga gtgattgatg gtaagtacta ttattttgat
    18301 tctctatctg gagagatggt tgtcggctgg caatatatcc cgtttccatc taaaggtagt
    18361 acaattggtc cttacccaaa tggtatcaga ttagaaggtt ttccaaagtc agagtggtac
    18421 tacttcgata aaaatggagt gctacaagag tttgttggtt ggaaaacatt agagattaaa
    18481 actaaagaca gtgttggaag aaagtacggg gaaaaacgtg aagattcaga agataaagaa
    18541 gagaagcgtt attatacgaa ctattacttt aatcaaaatc attctttaga gacaggttgg
    18601 ctttatgatc agtctaactg gtattatcta gctaagacgg aaattaatgg agaaaactac
    18661 cttggtggtg aaagacgtgc ggggtggata aacgatgatt cgacttggta ctatttagat
    18721 ccaacaactg gtattatgca aacaggatgg caatatctag gtaataactg gtactacctc
    18781 cgttcatcag gagcaatggc cactggctgg tatcaggaag gtaccacttg gtattattta
    18841 gaccagccaa atggtgatat gaaaacagga tggcaatacc ttggtaacaa gtggtactac
    18901 ctccgttcat caggagcaat ggtaactggc tggtatcaag atggtttaac ttggtactac
    18961 ctaaatgcag gtaatggaga catgaagaca ggttggttcc aggtcaatgg caaatggtac
    19021 tatgcttata gctcaggtgc tttggcggtg aatacgaccg tagatggcta ctacgtcaac
    19081 tataatggcg aatgggttcg gtaatgacag ggcgcttata attatattag aacactcgct
    19141 taatcaaaga agtgactttg ttgtacttga cctgacgatt ggggagatta aaagcatgtg
    19201 aaatctacac atgctttttc gttagttatg agtgttgaac ttgactaacc attgacaaaa
    19261 gataagagca atcaaaataa gatacaaaaa tatcaatgct aactgcaccg aaacgcccga
    19321 tgctatggac agggctaata gggagagcag ggacggggcg agaatgtcta ctgtgctcag
    19381 agcagagccc accgtcccca tcttgtcact ggggatatgg gcaaagacag ctgcttgtag
    19441 gcgagggctg agaatgccga taatcgtaga actgatgaaa ctggtcatga aaattacgat
    19501 ataggcttga caaaggatac tagttattag agccaatagg gaaatctcac agaaaaccaa
    19561 gaggtgcgtg aaacggatat ttttaaatag attactgctg gtcatattgc caaggatagc
    19621 tccacaagaa atgatgatgg agagcatggc gaggagttgc ccaatgctca gttgtgctag
    19681 tgtctgttgg atgaagcgga gggcaatcag ccgaggggac acatccaaga tggcaatcat
    19741 actggtcgtc agaaacagta gtatgacggt atcttttaac ctcaggagca cagccagcga
    19801 ctgtttaaga tgttgaaaat atttcttaaa actcagtgct gtatgtgaca tttcaatcct
    19861 tttttcaacc tcatacaagg tatgtctaat aacatacaga cccaaaaagg caatgacaaa
    19921 agtcagagtg ttgataaggg aaatagtttg aatactcata acattgataa ggaatgcgcc
    19981 agccagattg gcgacaatgt ggacaaccct catcagagac tgcctgaacc ccatggcatc
    20041 atgcaggtcg tccttaatta cactgatgta gagggcgttc atcatgtagg cagacaggta
    20101 gctcaaggtg tcagagatga ggttgatgat gacaatcata atgactgaaa ctaatgaaaa
    20161 gggcgacaag acgagaaata ctgtcaaaat agcgtagagc aggattttga ttttggcaat
    20221 ccagagtgcg tgtttgactc tattttcttg aaaatctcct agaatcccga gaaagagttg
    20281 cgatagtagg ggaaccatct ctgaaatgga aatcagtaaa atcgctagcg gagcgaaatt
    20341 gtactgagcc acataattga taatggcgag gtagaaaata ctgtcgccga atgatgaaaa
    20401 acagctgttt agcagcaact gtctatatat gttgtttgtc caaaataatt tcatactttt
    20461 ctccttatgt ctgcacatcg aatgggaatt tattatttaa tagacaccaa tataccccat
    20521 gcttcctatt ccgaagtcaa acaaatctgt tttaccacta tctccttgaa acatcagtaa
    20581 accgtgatca tctcgttcag aacatgctaa aattacctgt tggatttttt catcagtaat
    20641 ttgttttgat tgtagacagt ggtttgtagt aagctagtta ttccgtggca atatcctgtc
    20701 atcattttta gatgatggtt aaaaacaaat tcttgatatt tactaataat atctttgttt
    20761 ccgtcacaat cgtacataca aagatataag attattccgg aaattccttc gcaccaagaa
    20821 agttgtaaat ttcctaaatt ttcagtatta gaagtaactt tttctaataa tttttttaat
    20881 tcagcatgga atgtatggag atcattatag aacatagaag gttctaagac tttagaatag
    20941 gcaaataaag catatgcaat ttcacaatat ccatgagcaa agctatagtc taaaatgctt
    21001 tctttggctg tatctctttg taggaaataa tgataaattt ccccatatac tattgtggat
    21061 gaaagtttta agagtattgt cattggtaaa ttgatagtat tttattaatg ataataggac
    21121 accagatttt cccaatgcaa agtctatttc ctctagagta tcataattct ctattaattt
    21181 cttagcggtg acgtttgcta attgtagata gtattgattt ttactgatag aaaataaatg
    21241 taggagtaac catagatagc ctgcatcacc aaaagatagc ctgcatcacc aaatagcagt
    21301 gagtattgat attcatatgt gtgtgtcgtt ctaattgtca ctgccttctt gatgatagag
    21361 agtattttag aatccagttc tggggggtac aatttattta gaggaaacag tagtccagct
    21421 attccgtgtt ggaatgataa attacttacg aaatttccaa attctgttga agaagtgatc
    21481 cttccttcag aatttacagt tttatctagt aaaaatcgta gaacactatt acattctgtt
    21541 ttgtaatcat atttatgttc ctctattttt ttaattttga ctaaattatt tgtttgactt
    21601 aaatttagta gacattgtaa ccaaaatagt tgctcatctg tgagctgacc ttgagtatgt
    21661 gatagttcta gtaattgaat aggatccttt agttagattc aatcaatcta taacacaac
//
LOCUS       DAVKGU010000024        20959 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKGU010000024 DAVKGU010000000
VERSION     DAVKGU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.024"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            18..701
                     /locus_tag="VW077_000894"
     CDS             18..701
                     /locus_tag="VW077_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9454939.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            701..1435
                     /locus_tag="VW077_000895"
     CDS             701..1435
                     /locus_tag="VW077_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9454940.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            1589..2446
                     /locus_tag="VW077_000896"
     CDS             1589..2446
                     /locus_tag="VW077_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9454941.1"
                     /translation="MTQIIDGKALAVKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            2588..3460
                     /locus_tag="VW077_000897"
     CDS             2588..3460
                     /locus_tag="VW077_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9454942.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            3742..4425
                     /gene="rpiA"
                     /locus_tag="VW077_000898"
     CDS             3742..4425
                     /gene="rpiA"
                     /locus_tag="VW077_000898"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9454943.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            4439..5650
                     /locus_tag="VW077_000899"
     CDS             4439..5650
                     /locus_tag="VW077_000899"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9454944.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPDFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            5652..6209
                     /locus_tag="VW077_000900"
                     /pseudo
     CDS             5652..6209
                     /locus_tag="VW077_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            6209..7018
                     /locus_tag="VW077_000901"
     CDS             6209..7018
                     /locus_tag="VW077_000901"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9454945.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            7729..8478
                     /locus_tag="VW077_000902"
     CDS             7729..8478
                     /locus_tag="VW077_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9454946.1"
                     /translation="MTGHFLGCYLAQIAAVEAYQKYPDFYNHVLRRVTTFSAPKVITS
                     RTVWNAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTPLIHNDRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATFDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            8501..9016
                     /locus_tag="VW077_000903"
     CDS             8501..9016
                     /locus_tag="VW077_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9454947.1"
                     /translation="MCQHKLRLTIKKARYVRIELEGYNALSLAEVEVFRFIATNGETA
                     IQVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNG
                     RNQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTT
                     LNSKVSDDDVL"
     gene            9670..10380
                     /gene="deoD"
                     /locus_tag="VW077_000904"
     CDS             9670..10380
                     /gene="deoD"
                     /locus_tag="VW077_000904"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9454948.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKFGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKVVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(10403..10927)
                     /locus_tag="VW077_000905"
     CDS             complement(10403..10927)
                     /locus_tag="VW077_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9454949.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(10980..11216)
                     /locus_tag="VW077_000906"
     CDS             complement(10980..11216)
                     /locus_tag="VW077_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9454950.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(11284..12204)
                     /locus_tag="VW077_000907"
     CDS             complement(11284..12204)
                     /locus_tag="VW077_000907"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9454951.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            12402..12806
                     /locus_tag="VW077_000908"
     CDS             12402..12806
                     /locus_tag="VW077_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454952.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGICFVETYYGFTFLM
                     EHAIVTAVFIYSFSIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            13022..13603
                     /locus_tag="VW077_000909"
     CDS             13022..13603
                     /locus_tag="VW077_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9454953.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            13600..14877
                     /locus_tag="VW077_000910"
     CDS             13600..14877
                     /locus_tag="VW077_000910"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9454954.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLEDGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            14895..15557
                     /gene="deoC"
                     /locus_tag="VW077_000911"
     CDS             14895..15557
                     /gene="deoC"
                     /locus_tag="VW077_000911"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9454955.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            15544..15933
                     /locus_tag="VW077_000912"
     CDS             15544..15933
                     /locus_tag="VW077_000912"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9454956.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            16024..17076
                     /locus_tag="VW077_000913"
     CDS             16024..17076
                     /locus_tag="VW077_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9454957.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            17220..18755
                     /locus_tag="VW077_000914"
     CDS             17220..18755
                     /locus_tag="VW077_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9454958.1"
                     /translation="MAHENVIEMRDITKVFGGFIANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIHRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            18748..19806
                     /locus_tag="VW077_000915"
     CDS             18748..19806
                     /locus_tag="VW077_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454959.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            19809..20765
                     /locus_tag="VW077_000916"
     CDS             19809..20765
                     /locus_tag="VW077_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9454960.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            20850..>20959
                     /locus_tag="VW077_000917"
     CDS             20850..>20959
                     /locus_tag="VW077_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9454961.1"
                     /translation="MKLSYDDKVQIYELRKQGYSLEKLSNKFGINNSNLR"
BASE COUNT         6163 a         3694 c         4827 g         6275 t
ORIGIN      
        1 tagaaagcag tgtgactatg aatttttctt ttttacctaa gtatttacct tattttaact
       61 atggggctgt tgtgacgatt cttatttcta tctgtgttat ctttttggga actattttgg
      121 gtgttgtctt ggcttttggg caacgttcaa agtttaaacc gcttgtttgg ttggccaact
      181 tgtacgtttg gattttccgt gggacaccga tgatggttca aattatgatt gcctttgctc
      241 ttatgcatat caatgctccg actattcaga ttggaatttt aggtgttgat ttttcgcgtc
      301 tgattccagg gattttgatt atctctatga atagtggtgc ttatgtttcg gagactgttc
      361 gtgccggaat caatgcggtt ccaaaaggtc agctagaggc ggcttattcg ctagggattc
      421 gtcctaaaaa tgcgatgcgt tatgtgattt tgccacaagc aatcaaaaat atcttgccag
      481 cattggggaa cgaatttatc accattatca aggacagctc cctcttatca gctattgggg
      541 tcatggagtt gtggaatggg gctacaacag tttctacaac aacctatcta cctttaacac
      601 cacttttatt tgcagcattt tactacttga ttatgacctc tattctgaca gtagccttga
      661 aagcttttga aaaacatatg ggacaaggag ataagaaata atgacagaaa ccttgataaa
      721 aattgaaaat ttacataaat cttttggaaa gaatgaagta ttgaagggaa tcaacctcga
      781 gattaaaaga ggagaagttg tcgttatcat cggtccttca gggagtggga aatctacctt
      841 gcttcgctct atgaatttgt tggaggaagc aaccaagggg aagcttatct ttgagggagt
      901 cgatattacg gacaagaaga atgacttgtt tgccatgcgt gagaagatgg gcatggtttt
      961 ccaacaattt aacctctttc ctaatatgac tgtgatggaa aatattacct tgtcacctat
     1021 caagactaaa ggtgaaagca gggaagttgc tgagaagaga gcccaagagc ttttggaaaa
     1081 agttggtttg ccagataagg cagacgctta tccacagagt ctgtcgggtg ggcaacaaca
     1141 acggattgcc atcgcgcgtg ggttggctat ggaaccagat gttttgctct ttgacgaacc
     1201 aacttcagcc ttggatcctg aaatggtagg tgaggtattg gctgttatgc aagaccttgc
     1261 caagtcaggg atgactatgg ttatcgtaac acatgagatg ggatttgccc gtgaggtggc
     1321 agatcgtgtt atctttatgg cagacggtgt ggttgttgaa gatgggacac ctgagcagat
     1381 ttttgaacaa acccaagaac aacggactaa agatttcttg agtaaggttt tataatctat
     1441 tttaaaattt caagacaaga ttagtgaaaa gttcaaccag agctttttct tatagtttga
     1501 aactatagga taacctagga aagaagtgtt agagggcact agcagttttt ggtataatgg
     1561 aagatatttg aaagaaaaga gaagtgatat gacacagatt attgatggga aagctttagc
     1621 ggtcaaattg caggggcagt tggctgaaaa gactgcaaaa ttaaaggaag aaacagctct
     1681 agtgcctggt ttggtagtga ttttggttgg ggataatcca gccagccaag tctacgttcg
     1741 caacaaggag aggtcagctc ttgcggctgg ttcccgtagc gaagtagtgc gagttccaga
     1801 gaccattact caagaggaat tgttagacct gattgctaaa tataatcagg atccagcttg
     1861 gcatgggatt ttggtccagt tgccattacc aaaacatatc gatgaagagg cggttttatt
     1921 agccattgac ccagaaaagg atgtggatgg tttccatccc ttaaacatgg gacgtctttg
     1981 gtctggacat ccagtcatga ttccctcgac acctgcagga attatggaaa tgtttcatga
     2041 atatgggatt gacttggaag gtaaaaatgc ggtcgtcatc ggtcgttcaa atatcgttgg
     2101 aaaacctatg gctcagcttc ttttggctaa aaatgcgaca gtaaccttga cccactcacg
     2161 aactcataat cttgccaagg tggctgctaa agcagatatt cttgtagtcg caatcggccg
     2221 cgccaagttt gtgactgctg actttgtcaa accaggtgcg gttgtcattg acgttgggat
     2281 gaaccgagat gaaaatggga agctctgtgg agatgttgat tatgaggcag ttgcaccact
     2341 tgctagctat atcacgccag tccctggagg tgtcggtcct atgaccatta ccatgctgat
     2401 ggagcaaact tatcaagcag cacttcggac attggataga aaataagaga aacattcgat
     2461 taatagagtg tattttctat agctatatct aaaatggcag aaatgaatat taaattttag
     2521 atataagatt acaaaaggag gtctgcgtct cctttttatt gtataataaa agtgagagga
     2581 aaaaaggatg aaagtgatta atcaaacctt attagaaaaa gttattattg aacgttctcg
     2641 ttcaagtcat aaaggagatt atggtcgtct gctgctgctt ggtggtactt atccttatgg
     2701 aggtgccatc atcatggctg ctttagcagc tgtaaaaagc ggtgcaggat tggtgaccgt
     2761 tggaacggac agggaaaata tcccggctct gcacagccat ttacctgagc ctatggcctt
     2821 ttctcttcaa gaccagcaat tgttaaaaga gcaattggag aaggcagaag ttgtcttgtt
     2881 ggggcctggt ttacgagacg atgcttctgg agaaaatcta gtaaaacagg tctttgttaa
     2941 tttaagccaa aatcagattt tgattgtaga cggaggtgcc ttgaccattc ttgctaggac
     3001 aagtttgtca tttccctcaa gccagcttat cctaactcct caccaaaaag aatgggaaaa
     3061 attatctgga attacgattg aaaagcaaaa ggaagatgca acggctagtg ttctgacttc
     3121 cttccctcaa ggaacaattt tggtggagaa aggcccagct actcgtattt gggaagttgg
     3181 ccaatctgat tattaccagt tacaggttgg tggtccttat caggcgactg ggggaatggg
     3241 agatactctg gctgggatga tcgcaggatt tgtaggccaa ttccgacagg ccagtctcta
     3301 cgagcgtgtg gcagtagcga cccatcttca ttcagccata gcccaagaac tatctcaaga
     3361 aaattatgtg gtcttaccga cggaaatcag caggtatctt cccaaaataa tgaaaattat
     3421 ttgtcaacaa gagagggtaa gcaaagataa acttgtttaa aatagacaga taaatctact
     3481 tccttctctt aatttatgat tcttgaaatg cgcctgcttt aaggctaaac tagacgtatc
     3541 aaagttaaga gtttgttttt gtaactggaa agagatgaac aaatcaaata cataaaagat
     3601 aaactcattt ttattcgttt ggtaaaaata aaatgcatat ttttaaagaa agattgatga
     3661 aaaatgtaga tttgatgttt ttacttgaat aaaaagcggt ttcatagtaa aataagaact
     3721 gagaatgatg gaggtaaatc ggtggaaaat ctgaagaaaa tggcaggtat caaggctgct
     3781 gagttcgtga gcgatggaat ggtcgttgga cttggaacag gctcgactgc ctattatttt
     3841 gtcgaagaaa tcggtcgtcg tatcaaggaa gaaggattgc agattacagc tgtaacaact
     3901 tctagtgtga ccactaaaca ggcagaaggc cttaatatcc cgctcaagtc tattgaccaa
     3961 gtagactttg tcgatgtgac ggttgatggc gcggatgaag tggatagtca gtttaatgga
     4021 atcaaaggcg gtggtggtgc ccttctcatg gaaaaggtgg tcgcaacacc atcaaaagaa
     4081 tacatttggg tggtggatga aagcaagctg gtcgaaaaac taggtgcttt taaattgcca
     4141 gtagaagtgg ttcagtatgg tgcagagcag gtctttcgtc attttgaacg agctggctac
     4201 aaaccaagtt tccgtgaaaa agacggccaa cgttttgtga cggatatgca gaattttatc
     4261 attgacctcg ccttggatgt cattgaaaat ccaattgctt ttggacaaga attggaccat
     4321 gtcgttggtg ttgtggagca tggtttattc aaccaaatgg tggataaggt aatcgttgct
     4381 ggacgagatg gagttcagat ttcaacttca aaaaaaggaa aatagaaggg ggcataagat
     4441 gtctaaattt aatcgtattc atttggtggt actggattct gtaggaatcg gtgcagcacc
     4501 agatgctaat aactttgtca atgcaggggt tccagatgga gcttctgaca cactgggaca
     4561 catttcaaaa acagttggtt tgaatgtccc aaacatggct aaaataggtc ttggaaatat
     4621 tcctcgtgaa actcctctta agactgtagc agctgaaatc aatccaactg gatatgcaac
     4681 aaaattagag gaagtatctc ttggtaagga tactatgact ggacactggg aaatcatggg
     4741 actcaacatt actgagcctt tcgatacttt ctggaacgga ttcccagaag aaatcctgac
     4801 aaaaatcgaa gaattctcag gacgcaaggt tattcgtgaa gccaacaaac cttattcagg
     4861 aacggctgtt atcgatgatt ttggaccacg tcagatggaa actggagagt tgattatcta
     4921 tacttcagct gaccctgttt tgcagattgc tgcccacgaa gacattattc ctttggatga
     4981 attgtaccgt atctgtgaat acgctcgttc gattaccctt gagcgtcctg cccttcttgg
     5041 tcgcatcatt gctcgccctt atgtaggtga accaggtaac ttcactcgta cggcaaaccg
     5101 tcgtgacttg gctgtatctc cattttcccc aactgttttg gataaattga atgaggctgg
     5161 tatcgatact tatgctgtgg gtaaaatcaa cgatatcttt aacggtgctg gtatcaacca
     5221 tgacatgggt cacaacaagt caaatagtca tggaattgat acactattga agactatggg
     5281 acttgctgag tttgaaaaag gattctcatt cacaaaccta gttgactttg atgcccttta
     5341 cggccatcgt cgtaatgctc acggttaccg tgattgtttg catgagtttg atgaacgctt
     5401 acctgaaatt atcgcagcta tgagagagaa tgaccttctc ttgattactg cggaccatgg
     5461 aaatgaccca acgtatgcag ggacggatca cactcgggaa tatattccat tgttggcata
     5521 tagccctgac tttaaaggaa atggtctcat tccagtagga cattttgcag atatttcagc
     5581 gactgttgcc gataactttg gtgtggaaac tgctatgatt ggggaaagtt tcttagataa
     5641 attggtataa gatgacgcgc tatgctttgc tggtgagagg tatcaatgtt ggtagtaaga
     5701 ataaggtcgt tatggcggag cttcgtcaag aattgacaaa cttgggactg gaaaaggttg
     5761 agagctacat caacagtggc aatattttct ttacttcgat agattccaaa gcccaattgg
     5821 tttaaaagct agagactttc tttgcagtcc attatccatt tattcagagc ttttctttac
     5881 tgagtctaga ggactttgag gcggaacttg aaaatctacc agcttggtgg agcagagact
     5941 tggcacgaaa agattttctc ttttacactg agggtttgga tgtggaccaa gtcatcgcga
     6001 cagttgaaag tttagagctg aaagatgaag tgctttattt tagaaaactt gggattttct
     6061 gggggaaatt ttctgaagaa tcctattcta agactgccta tcataagtac ttgctgaagg
     6121 tgcctttcta ccgccacatt actattcgta atgctaaaac ctttgacaaa attggtcaaa
     6181 tgctaaaaaa taataaagga gacacataat gacattttta aacaaaatcc atgaaactgc
     6241 tactttcctg aaagaaaagg gaattgcagc ccctgagttc ggtctaatcc ttgggtcagg
     6301 acttggagaa ttggcagaag aaatcgaaaa tccagttgta gtagactatg ctgagattcc
     6361 aaactggggc cgttcaacag tagtcggtca tgctggtaaa ttggtatatg gtgaactggc
     6421 aggtcgcaag gtcttggctc ttcaaggtcg tttccatttc tatgaaggga atcctctgga
     6481 agtggtgact ttcccagttc gtgtgatgaa agttcttgga tgtgaaggtg ttattgtaac
     6541 caatgcagct ggcggtatcg gatttggtcc tggtaccttg atggctatct cagaccatat
     6601 caacatgacg gggcaaaatc cattgatggg tgaaaacttg gatgactttg gcccacgttt
     6661 cccagatatg tctagggcct acacaccaga ataccgtgcc actgcccatg aagtggctaa
     6721 aaaacttaat atcaagcttg atgaaggtgt ctatatcgga gttactggtc cgacttatga
     6781 aacaccagca gaaattcgtt cctataagac actgggagca gatgcagttg gtatgtctac
     6841 ggttcctgaa gttatcgtgg cagcccactc tggcttgaaa gttctaggaa tttcatgtat
     6901 cactaacttt gcggctggtt tccaagaaga actcaatcac gaagaagttg tagaagtgac
     6961 tgaacgtgtt aaaggtgatt tcaaaggctt gcttaaagcg attcttgctg aattgtaaga
     7021 aaaaagattt aaaaggggaa gtacctctgt tttttcagga ttgactgcct atccggatta
     7081 aagaagaaac agaggaatac tatgagcttc ttcctgctct tataactgaa agaagcggaa
     7141 gaataggtat gtctgatctg atagccagca ttgtgaaaga caagattcta ggatactagc
     7201 attagcttcc tagccaagca gactagtatg ataaggagag atgagaatga attgactttc
     7261 tgaatttctc agtcttatca tatatagcac aatgagattt cgcttgagtc tgcttgtaaa
     7321 taaacgaaaa gaaagataag aaataatgaa aattggtcaa cgaattatgc gctttggcat
     7381 aaaaaattaa gtatcggagt tgtatctgtt gtagtcggct ttgatttcta gctccagctg
     7441 gaatttcagc caatgaagta aagtaagatg taacatctga agtggtaata ggtgtgttag
     7501 attctaagga ggaattgaaa gagtcagaaa atgatgctct aaaactagaa actcctctta
     7561 gagaggagcc aagactagct cctcaaacgc ttccggaagc aagtgaagtt agttcttgaa
     7621 aacaaaaggg aagagtcaaa agtagagata acagaaccag ctcaagcgga tgatatccgc
     7681 aaggttgttg gggaattagc caaggatata agtattacta agttgtatat gacaggtcat
     7741 tttcttggat gttacctagc tcagattgca gcggttgaag cttaccaaaa atatcctgat
     7801 ttttataacc atgtattgag gagagtgaca actttcagtg ctcctaaagt gattacttcc
     7861 agaactgttt ggaatgctaa gaatggtttc tgggatgttg gtttggaaag tcgtaaatta
     7921 gctgttagtg gaaaaattaa gcattatgtg gttgataatg acaatgttgt gactcccttg
     7981 attcataatg atcgtgatat tgttacattt acaggtaatt cacgctttaa acaccgttct
     8041 cgtggctatt ttgaaagtcc aatgaatgat attcctaact ttaatattgg taaacaagct
     8101 acctttgata aacatggtta tcgtgatccg aaattggata aagtgcgatt ctttaagaaa
     8161 caggctctac ctcaatcttc tagtcaacca agcgctgaac caatggaaaa tattgcctta
     8221 ggaaaacagg ttactcaaag ttcgacagct ttcggaggag atgctagaag agctgtggat
     8281 ggcaaagtcg atggtaacta tggtcacaat tctgtcactc atacaaactt ccaatctaag
     8341 ccttggtggc aagtagattt ggctaaagaa gaaaccattc gccaaatcaa tatttacaac
     8401 cgaacagaca ctgcccagga tagattggca aactttgatg tcattctttt agacagttct
     8461 ggtaaagaaa ttgagtgaaa acgtataaca tctcctaaag atgtgtcagc acaaattacg
     8521 attaaccata aaaaaagcgc gctatgttcg gattgagcta gaaggctata atgccctcag
     8581 tcttgcagaa gttgaagttt tccgctttat agctacgaat ggtgaaacgg cgatacaagt
     8641 ttctaagtca gttcaaccaa tcagtcagac tcctgtgaaa gataaaacat tgacaattca
     8701 acacagtgga gcttacattg cccgctactc cataacttgg gaagaagtta cagtagataa
     8761 agatggaaac caagttgttc gtagtcattc ttgggaagga aacggtcgca accagactgc
     8821 aggttttgtc ctcaacctcc caatcaaaga aaatatgaga aatctgcgag ttaagattga
     8881 gaaaaagacg ggcctactat ggaatagatg gcaaacaatc tatgaaaaca gaccaatttt
     8941 agctcaaccc caccgtaaaa ttacccattg gggtacgaca ttgaattcca aggtgagtga
     9001 cgatgatgtc ttgtaatctg atggtagaat gacagttagt ttgtctagtt tataagaaag
     9061 tactacctga gcttgaatag aactcaggta gctctctatg aaagaacaaa attaatactc
     9121 aatgaaaatc aaagagcaaa ctaggaaact agccgcaggt tgctcaaagc actactttga
     9181 ggttgtagat aagactgacg aagtcagtca catatataat ccaaggcgac gttgacgtgg
     9241 tttgaagaga ttttagaaga gtataaacag aaaggtagag cgcgtgttct aatttgaaca
     9301 cgagtagaaa acttttctaa aaacaaaaac gaaaggatgg gtaaactgta ttcgctgaac
     9361 tgaatacggg cgactctcct ctaaatcaaa attaagaaag gaattgaccc caccctaaaa
     9421 gtagtgggaa aaagatagtt ggtctagcga gcatcgctca ctgcgcccaa ctcctatttt
     9481 cccttcgctt tttgatgggt ttggtatctt tctcaatata aaatataaaa taaagaaagg
     9541 tagagcgcgt gttctaattt gaacacgagc ggaaaactcg gaaaatagat aatctgactg
     9601 aaaaatcagg atttctcgtc aggttcctaa ttttcagtcg ttttcttctc gctctttgta
     9661 tcataaatta tgtctatcca tattgctgct cagcagggtg aaattgctga taaaattctt
     9721 cttcctgggg atcctcttcg tgctaagttt attgcggaga atttccttga tgatgctgtt
     9781 tgttttaacg aagtgcgtaa catgtttggt tacactggta cttacaaggg tcactgtgta
     9841 tctgtcatgg gaactgggat gggaatgcca tctatttcga tttatgcgcg tgagttaatc
     9901 gtagactacg gtgtgaagaa attgattcgt gtgggaactg caggttcttt gaatgaagag
     9961 gttcatgttc gtgaattagt tttggcgcag gcggctgcaa ccaactcaaa catcgttcgt
    10021 aatgactggc cacagtacga ttttccacaa attgctagct ttgatttgct tgataaagcc
    10081 taccatatcg ccaaaaaatt tggtatgact actcacgttg ggaacgtttt gtcatctgat
    10141 gtcttttact caaattactt tgaaaagaat atcgagcttg gtaaatgggg agtcaaggtt
    10201 gtggaaatgg aagcagcagc tctttactat cttgctgccc aataccatgt tgatgcgcta
    10261 gctatcatga ccatctctga tagcttggtc aatccagacg aagacacaac tgcagaagaa
    10321 cgtcaaaata ccttcactga tatgatgaag gttggtttgg aaaccttgat tgcagaataa
    10381 ttatagccaa aaaggaagcc ctttatgaat tgtaggactt ccttttctta tccagaaatt
    10441 gatctagttc tctctgattt cgaagaatag tgactttatg tgaatattct tggcaaagtt
    10501 tttggtaatt ttctttttga gttttgctac gcccatccca aagaatccat ctgataaact
    10561 cccactcaaa gcgttcaggg caatctgccg ccatactttc tctgactttt ccacggtatt
    10621 taagataacg cttaaaggct ctaaagagac aggtcaatgg caaaaaattg agaaagatga
    10681 tttggtcagc ttcttgcatt cgttcttggt agtagcacca agaataatta ccatcgatga
    10741 cccaagcttt atgcttggtg agaaagtttt ttatctcggt taacatccat tcgcagtcac
    10801 tgtcttgcca accaggttga aattggagtg tgtccatgtg cagttttgga atggagtagt
    10861 agttagataa cttttctgct agagttgact taccagaacc agaatatccg ataattgtga
    10921 ttttcatttt ctaccttttc ctatttggag acaaaaaaac agcctctatg gactgtttct
    10981 tatttagcaa gtttagctga aagacgagct ttatcgcggc ttgctttgtt tttgtgaatc
    11041 aaacctttag tttctgcttt atcgatagct gagctagcag cacggaaaag ttcttcagat
    11101 gggtttgctt caaaagcttt tatagcagta cgcatagctg atttttgagc tgagttcttt
    11161 tcgttttgtt taacgttcaa ttcagcgcgt ttgatagctg atttaatgtt tgccaatgtt
    11221 cttacctcca tatttactaa ctataccatt atatatgaaa acttacgttt tgacaaggga
    11281 aaattatttt tttaagtaaa tttcatcgat ttcatggttc ttgcttttat gaagaatcac
    11341 ttctgcacga tttctggttg gttcaatata attttgtaga tttgtgagat tgatactggt
    11401 ccagacctga tgggcaaagg cttccacttc cccaatcggc atctgagtaa aacgataata
    11461 gtagctatca gggtcgtttt gggctagact cagcattttc aagaaacggt ccagatacca
    11521 actttcaata tcatccactc cagcatctac atagatggaa aagtcaaaga agtcagtgat
    11581 atagagacga tcgttttgtg gattttgaaa gacattgatt ccctcaacaa ttacaaaatc
    11641 agcagctttg acactttgtt tctcttcggg tacgatgtcg taaacttcat gagaatagac
    11701 aggaatatct acatcttgtc catttttgat gcggtccaag aagttgagaa gagcttccat
    11761 atcatagctt tcaggaaatc ctttacgatt taaaatcccc tgctcaatca aggtttgatt
    11821 gggatagaga aaaccatcag ttgtaaccaa ctcaaccgta gcatctgtaa acgtacggga
    11881 cagtaggatt tgaagtaggc gactggttgt ggattttcca acggcaacac tcccagaaac
    11941 cccaataata aaaggttgag atttactttc acgttggagg aaaattcctt ttgaaaaggc
    12001 taaatcttcc ttagttcgct tgtaaatctg aatcagatga gccaagggga gatagatatc
    12061 tgtaacgtct tggagactga tttggtcatt aaaactcttg atagattcca attcttcttc
    12121 tgtcaaagga ggtgttgtct ttcgatgtaa agattgccaa gtctggcggc tgattttttc
    12181 aaaatgtaaa aattcgttgg tcatttgttt tcccctagat attttgtcta tcataccata
    12241 aaaatctaaa aaaataaagc ggtttcttga atgtggtaag tcaatggtat atagtataag
    12301 tgatcaattt attgatgaaa taggagatag attctactga tttataagtt tataagctta
    12361 taaagggggc tttttagttc acaacttata ggaggtgtac tatgaaaatc ttaaaacgtt
    12421 acatattgga actctgtttt attttaagtt ttgctttacc ttttataaaa ggagccaatg
    12481 cagataatgg tatatgcttt gtggaaacct attacggttt tacttttttg atggaacatg
    12541 ctattgtaac agctgtcttt atctattcgt tttcaattgc tttcttacta aaaaaacgat
    12601 gggcgaaatg gattgctgct ggtagttatt gctttttagt cctatggatt gcgacagagg
    12661 gttatttctt ccgcatgtca ctagaagatt tgatacgact ttggacaagt ttggaaatcc
    12721 tgacacaaac gtatcagttg ggcttttatc taaacatttt gttgggaatt ttgttgataa
    12781 taaagtattt taaggttaag caatagaaat aataatctgc ctgattatag atgataaact
    12841 ttttgttttt atctggactg tgatatttat agttctaaat ggtttattat ttataagtgt
    12901 tgaaagagat tgaagaaaat ggcttcgtga ctcgctttct tcggtctttt tcgttttatt
    12961 atcaatttgt aaacgattta caatttgagt caaattatgg taaaatagtg tcatgagtaa
    13021 aatgtattat gcagaaaatc ctgacgctgc tcacgacatt catgagttga gagtagagtt
    13081 gttgggacaa aaaatgacct ttttaacgga tgcgggtgtt tttagcaaga aaatagttga
    13141 ctttggaagt caactattgc tcaagtgtct tgaggtcaac caaggagaaa ctgtccttga
    13201 tgtaggctgt ggttacggac ctttgggttt gtccttagct aaggcttatg gagttcaggc
    13261 tactatggtt gatatcaaca ctcgtgcctt agatttagct cggagaaatg ctgaaaaaaa
    13321 taatgcaaaa gcgacgattt tccagtccaa catctatgaa caagttgaag gacattttga
    13381 tcatgtcatt tccaatcctc ctatccgtgc gggcaagcaa gtcgttcatg aaatcattga
    13441 gaaaagtaaa gatttcttgg aaactggtgg ggatttgaca atcgtcatcc agaaaaaaca
    13501 aggggctcca agtgctaagt ccaagatgga ggatgtgttt ggtaattgtg aaatccttaa
    13561 aaaagacaaa ggatactata tccttagaag tgtgaaagaa tgagagcagt tgatttaata
    13621 caaaaaaaac gagacggtca agaactgact tcaagtgaaa ttgaatggct ggtagaaggc
    13681 tatgtgtcag gaactgttcc tgattatcag atgtctgcct ttgctatggc tgtttatttt
    13741 aaaggaatga cgactcgtga aatttctgat ttaacgatga atatggttaa gactgggcaa
    13801 gagtttgacc tgtcagccat tgatggtgtg aaagttgaca agcattctac tggtggtgtc
    13861 ggcgataagg taaccttgat tttagctcct cttgttgcta gcttcggtgt gcctgtagca
    13921 aaaatgagtg gtcgtggtct tggccataca ggcggaacaa ttgataaatt ggagtccatt
    13981 aggggctatc aagtagaacg tagtcaagag gattttattc gtcaggttca ggatattgga
    14041 gtatctgtca ttgggcaatc agaccagctg gtcaaagcag ataagcttct ctatgccctt
    14101 cgtgatgtga ccgcaactgt cgacacgatt cctttgattg cgagttcggt gatgagcaag
    14161 aaaatcgcgg caggggcgga tgctattttg ctagacgtga ctgtcggtga gggtgccttc
    14221 atgaagacgg ttgatgaggc gcgtgagttg gctcaaacta tggtagatct tggtaaggtt
    14281 gttggacgga agacagtagc agtgattacg gatatgagcc agcctttggg acgagcgatt
    14341 ggaaatcgtc tggaaatcct tgaagcattg gagattttgc aaggtcaagg acgtcaggat
    14401 attacccact ttatttgtga attggctcaa attatgcttg gcttggcaaa tgttaacaag
    14461 actgttgaag aagttcgcca acatcttgag gatggtcaag cactggctaa gtttgaggaa
    14521 atggtgcaag cccaaggtgg agacttggaa gacctctatc gtcctgtaaa tgtagctcat
    14581 gtggtggaaa tccctgctca ggaaacaggt gtcatttcag ctcttccagc tatggacttt
    14641 ggtctttatg ccatgaggct gggtgctggt cgtgcagtta agtctgatgc cttggactat
    14701 gaaacaggaa ttgtttttga aaagaagatt ggagactccg ttcaaaaggg tgaaattgtt
    14761 gcaaaagtat acacaaatga aaaaattcca cctcaactag ttacagattt ccaaaaatgt
    14821 gttaaaataa gtgatgaagt gaaaaaaatc cgtgaaattg tagaaatcat ctcctaaatg
    14881 gagggaaaac cgaaatgaaa ttaaataaat atatagatca tacgctttta aaacaagatg
    14941 caaagaaaaa acaaattgat agtttgttgt ctgaggctag agagtatggc tttgccagtg
    15001 tttgcgttaa tccaacctgg gttgaacatg ctaaaaaagg gcttgaaggc acagatgtta
    15061 aggtttgcac ggtagtaggt ttccctttgg gagcaacaac ttcagccgtg aaagcatttg
    15121 agacaaaaga agctatccaa aatggtgcag atgagattga tatggtgatc aatgttggag
    15181 ctctcaaatc aggtaattta gccttggttg agtcagatat tcgcgcagta gtggaagcaa
    15241 gtggtgataa gttagtgaaa gtcattattg aagcttgcct tctgacagac caagaaaaag
    15301 ttgttgtttg ccaattggcc caaaaagctg gggctgactt tgtcaaaaca tctactggct
    15361 tttcaactgg tggtgctacg atagcagatg ttacattaat gcgtgaaaca gttggatctg
    15421 atatgggagt caaggctgct ggtggagctc gttcttatgc agatgctctt gcctttgtcg
    15481 aagcaggtgc gacccgtatc ggaacgtcag ctggggtagc tattttaaaa ggagaattgg
    15541 cagatggcga ctactgagtt gattgaactg gcaattgaaa ccagcaaaca tgcctatgtc
    15601 ccctattctc actttcctat cggagcagtt ttagtagcca aagacggaaa tgtttacacg
    15661 ggagtgaata tcgaaaatgc tagctatcct ttgaccaatt gcggtgagag aacagctatt
    15721 tttaaggcta tatctgaagg ccaaagagaa ttttcagaat tgattgtcta tggtcaaact
    15781 gaaaaaccga tttcaccatg tggtgcttgt cgccaagtaa tggtcgaatt ttttgaacaa
    15841 gatttaaaag tgaccttagt cgcaaaagat aaatcgacgg tcgagatgac ggtcggggag
    15901 ttacttccat attcttttac agacttaaat tagtctgagt cgctctttga gtggcaaggg
    15961 tctttgtgac caatcaatcc atacttgcaa catcgttgca catcttattt aggaggttca
    16021 gtaatgaaca agaaacaatg gctaggtctt ggcctagttg cagtggcagc agttggactt
    16081 gctgcatgtg gtaaccgctc ttctcgtaac gcagcttcat cttctgatgt gaagacaaaa
    16141 gcagcaatcg tcactgatac tggtggtgtt gatgacaaat cattcaacca atcagcttgg
    16201 gaaggtttgc aggcttgggg taaagaacac aatctttcaa aagataacgg tttcacttac
    16261 ttccaatcaa caagtgaagc tgactacgct aacaacttgc aacaagcggc tggaagttac
    16321 aacctaatct ttggtgttgg ttttgccctt cacaatgcag ttgaagaagc agctaaagaa
    16381 cacactgact tgaactatgt cttgattgat gatgtgatta aagaccaaaa gaatgttgcg
    16441 agcgtaactt tcgctgataa tgagtcaggt taccttgcag gtgtggctgc agcaaaaaca
    16501 actaagacaa aacaagttgg ttttgtaggt ggtatcgaat ctgaagttat ctctcgtttt
    16561 gaagcaggat tcaaggctgg tgttgcgtca gtagacccat ctatcaaagt ccaagttgac
    16621 tacgctggtt catttggtga tgcggctaaa ggtaaaacaa ttgcagccgc acaatacgca
    16681 gccggtgcag atattgttta ccaagtagct ggtggtacag gtgcaggtgt ctttgcagag
    16741 gcaaaatctc tcaacgaaag ccgtcctgaa aatgaaaaag tttgggttat cggtgttgac
    16801 cgtgaccaag aagcagaagg taaatacact tctaaagatg gcaaagaatc aaactttgtt
    16861 cttgtatcta ctttgaaaca agttggtaca actgtaaaag atatttctaa caaggcagaa
    16921 aaaggagaat tccctggcgg tcaagtgatc gtttactcat tgaaggataa aggggttgac
    16981 ttggcagtaa caaacctttc agaagaaggt aaaaaagctg tcgaagatgc aaaagctaaa
    17041 atccttgatg gaagcgtaaa agttcctgaa aaataatgga tggaagtcat ttcttaggaa
    17101 gcggccaagg gccgcttttt taagagttga gacaaagtca ttttgtctca gtaaagcttg
    17161 ctactagaca aactagcaag ttttattgaa ataaaataag actctgaaag gaagagcaca
    17221 tggcacacga aaatgtcatt gagatgcgtg atattaccaa ggtgtttggt ggatttattg
    17281 ccaacgacaa aatcaacttg cacctacgaa aaggtgaaat tcatgcactt ttaggagaaa
    17341 atggggctgg taagtccacg ctaatgaaca tgttagcagg ccttcttgaa ccaactagtg
    17401 gtgaaatcgc ggtcaacggt caagttgtca atctcgactc cccatctaaa gcagctagct
    17461 tgggaatcgg gatggttcac cagcacttta tgttggttga agccttcaca gtggctgaaa
    17521 acatcatttt aggtagtgaa ttgactaaaa atggtgtgct agatatcgct ggagctagca
    17581 aagaaatcaa ggctctttct gaacgttatg gcttagctgt tgacccttct gccaaggtag
    17641 cagatatctc agttggagcc caacaacgtg tagaaatttt aaaaacactt tatagggggg
    17701 ctgatatcct tatctttgac gaaccaacgg ctgttttgac tccatcagaa attgatgagt
    17761 tgatggctat tatgaaaaat cttgtcaaag aaggaaaatc aattatcttg attacccaca
    17821 aattggatga aattcgcgca gtttctgacc gtgttacagt tatccatcgt gggaaatcaa
    17881 ttgaaaccgt tgaaattgca ggggctacca atgctgattt ggcggaaatg atggtaggac
    17941 gttctgtttc ctttaaaaca gagaagcaag cctctaaacc aaaagaagtg gttttgtcta
    18001 tcaaagattt ggtggtcaat gaaaaccgtg gtgttccagc tgttaaaaat ctgtccttgg
    18061 atgttcgtgc tggagagatt gttggtattg cggggattga tggaaatggt cagtctgaac
    18121 tgattcaagc cattacaggt cttcgtaagg ttgaatctgg tagcattgag ctaaaaggag
    18181 attcaattgt aggcttgcac ccacgtcaga ttacagaact aagtgttggg cacgttccag
    18241 aagaccgtca ccgtgatggc ttgattttgg aaatgatgat atctgaaaat attgcccttc
    18301 aaacctacta taaagaacca catagtaaaa atggaatttt gaactattca aatattactt
    18361 cttatgctaa aaagctgatg gaagagtttg atgttcgcgc tgccagtgaa ttagttcctg
    18421 cagctgcact ctcaggagga aatcaacaaa aagcaattat tgctcgtgaa attgatcgag
    18481 atcctgatct ccttatcgtt agccagccaa ctcgtgggtt ggatgtcggt gccattgagt
    18541 atatccacaa acgcttgatt gaagagcgtg ataatggcaa ggctgtcctt gttgtcagct
    18601 ttgaattgga tgagatttta aacgtctcag accgtattgc cgttatccac gatggtaaga
    18661 ttcaaggtat tgtatcacca gaaacaacca ataaacaaga acttggtgtc ttgatggctg
    18721 gtggaaactt gggaaaggag aagagtgatg tctaaaaaat tacaacaaat ttcggttccc
    18781 ttgatttctg tatttctagg aattttactc ggagccattg tcatgtggat cttcggttat
    18841 gatgctattt ggggctacga agaattattc tatacagcct ttggcagtct gcgtgggatt
    18901 ggagaaatct tccgtgctat gggtcctctg gtcttgattg gtcttggttt tgcggttgcc
    18961 agtcgagctg gtttctttaa cgtcggactt cctggtcagg ctttggcagg ttggattctc
    19021 agtggttggt ttgcgctgtc gcatccagat atgccccgtc ccttgatgat tctagcaacc
    19081 atcgtgattg ccttgattgc tggtgggatt gtcggagcga ttccaggtat tcttagggcc
    19141 tatctaggga cgtcagaggt tattgtaacc atcatgatga actacattgt cttgtatgta
    19201 gggaatgcct ttatccatgc tttccctaaa gacttcatgc aaagtacaga ttcgaccatt
    19261 cgtgttgggg ctaatgcaac ctatcagaca ccttggttgg ctgagttgac tggtaactca
    19321 cggatgaata ttggtatttt ctttgccatc attgccgttg cagttatttg gttcatgctc
    19381 aagaaaacaa ctcttggttt tgaaatccgt gcagttggtc ttaatccaca tgcttcagaa
    19441 tatgctggta tttctgccaa gcggactatt atcctatcta tgattatttc aggtgccttg
    19501 gcaggtcttg gtggagctgt tgaaggtttg ggaaccttcc agaacgtcta tgttcaaggt
    19561 tcgtcattag ctatcggatt taacggaatg gcggttagtt tgcttgcggc caactcacca
    19621 attggtatac tctttgcagc cttcctattt ggcgttctcc aagttggggc tcctggtatg
    19681 aatgcggcgc aggtaccatc tgagcttgtc agcattgtaa cagcgtctat tatcttcttt
    19741 gtcagtgttc attaccttat cgaacgcttt gtcaaaccga aaaaacaagt taaaggaggt
    19801 aagtaaagat gtctattata accttgctcc ccctcttggt gtcttctatg ctgatttact
    19861 cagcacctct catctttaca agtatcggtg gtgttttctc tgaacgtggt ggtgtggtaa
    19921 acgttggcct tgaaggaatt atggttatgg gtgccttttc tggagttgtc tttaaccttg
    19981 aatttgcaga acaatttgga gcagcaactc catggctatc cttgcttgta gcaggattgg
    20041 ttggtagtgt tttctctatc atccacgcag cagcgacggt tcatttccgt gcagaccatg
    20101 ttgtcagcgg tacggtattg aacttgatgg cgcctgcctt ggctgttttc ttagttaaag
    20161 ttctttataa caaaggacaa accgacaacc taagtcaaac ttttggacgc tttgatttcc
    20221 cagtcttggc aaatatccca gtgattggtg atatcttttt caagtcaact agtctacttg
    20281 gttatctggc gattgccttc tcattccttg cttggtttat tctctttaag actcaatttg
    20341 gtcttcgtct ccgctctgtc ggtgaacatc ctcaagcagc ggataccttg ggaatcaacg
    20401 tctacaagat gagatattta gggattatta tttcaggttt tctaggtgga attggcggag
    20461 cgatttatgc gcaatcaatc tcagttaact tctcagtgac aactattgtt ggacctggat
    20521 ttatcgccct tgctgcgatg atctttggga aatggaatcc aatcggtgct atgctttcta
    20581 gtctcttctt tggactttca caaagtttgg ctgttatcgg ttctcaattg ccgttcctac
    20641 aaggagtgcc agcggtttat cttcaaattg caccttatgt tttgacaatt cttgtcttag
    20701 cagccttctt tggaaaagca gtcgcaccaa aagcagatgg tatcaactac atcaaatcaa
    20761 aataagcata caaaaaaacg tcagtccaat gactgaactg ccccccaaaa gttagacaga
    20821 aaaaatctaa cttttggggt gtttttatta tgaaattaag ttatgatgat aaagttcaga
    20881 tctatgaact aagaaagcaa ggatatagct tagagaagct ttcaaataaa tttgggataa
    20941 ataattctaa tcttaggta
//
LOCUS       DAVKGU010000025        20659 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKGU010000025 DAVKGU010000000
VERSION     DAVKGU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.025"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(69..2327)
                     /gene="glgP"
                     /locus_tag="VW077_000918"
     CDS             complement(69..2327)
                     /gene="glgP"
                     /locus_tag="VW077_000918"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9454962.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGDENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2353..3870)
                     /gene="malQ"
                     /locus_tag="VW077_000919"
     CDS             complement(2353..3870)
                     /gene="malQ"
                     /locus_tag="VW077_000919"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9454963.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            4420..5691
                     /locus_tag="VW077_000920"
     CDS             4420..5691
                     /locus_tag="VW077_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9454964.1"
                     /translation="MSSKFMKSAAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            5802..7094
                     /locus_tag="VW077_000921"
     CDS             5802..7094
                     /locus_tag="VW077_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9454965.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            7096..7938
                     /locus_tag="VW077_000922"
     CDS             7096..7938
                     /locus_tag="VW077_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9454966.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            8199..8999
                     /locus_tag="VW077_000923"
     CDS             8199..8999
                     /locus_tag="VW077_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9454967.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            9009..9995
                     /locus_tag="VW077_000924"
     CDS             9009..9995
                     /locus_tag="VW077_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9454968.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            10131..10211
                     /locus_tag="VW077_000925"
                     /pseudo
     CDS             10131..10211
                     /locus_tag="VW077_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(10421..11362)
                     /locus_tag="VW077_000926"
     CDS             complement(10421..11362)
                     /locus_tag="VW077_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9454969.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLTFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(11340..13103)
                     /gene="aspS"
                     /locus_tag="VW077_000927"
     CDS             complement(11340..13103)
                     /gene="aspS"
                     /locus_tag="VW077_000927"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9454970.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITESLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVRGVDFKVFSEAPAVKAIVVTGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(13339..13419)
                     /locus_tag="VW077_000928"
                     /pseudo
     CDS             complement(13339..13419)
                     /locus_tag="VW077_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(13455..13685)
                     /locus_tag="VW077_000929"
     CDS             complement(13455..13685)
                     /locus_tag="VW077_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454971.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(13703..14344)
                     /locus_tag="VW077_000930"
     CDS             complement(13703..14344)
                     /locus_tag="VW077_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9454972.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(14356..15090)
                     /locus_tag="VW077_000931"
     CDS             complement(14356..15090)
                     /locus_tag="VW077_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9454973.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NIIGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(15093..15290)
                     /locus_tag="VW077_000932"
     CDS             complement(15093..15290)
                     /locus_tag="VW077_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454974.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(15290..15496)
                     /locus_tag="VW077_000933"
     CDS             complement(15290..15496)
                     /locus_tag="VW077_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9454975.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(15506..15820)
                     /locus_tag="VW077_000934"
     CDS             complement(15506..15820)
                     /locus_tag="VW077_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454976.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(16090..16170)
                     /locus_tag="VW077_000935"
                     /pseudo
     CDS             complement(16090..16170)
                     /locus_tag="VW077_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(16161..17450)
                     /locus_tag="VW077_000936"
     CDS             complement(16161..17450)
                     /locus_tag="VW077_000936"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9454977.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFATKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(17528..18775)
                     /locus_tag="VW077_000937"
     CDS             complement(17528..18775)
                     /locus_tag="VW077_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9454978.1"
                     /translation="MKLLFRNPAYRILILSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNAYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(18968..19819)
                     /gene="rgg"
                     /locus_tag="VW077_000938"
     CDS             complement(18968..19819)
                     /gene="rgg"
                     /locus_tag="VW077_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9454979.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            19943..20284
                     /locus_tag="VW077_000939"
     CDS             19943..20284
                     /locus_tag="VW077_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9454980.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            20342..20422
                     /locus_tag="VW077_000940"
                     /pseudo
     CDS             20342..20422
                     /locus_tag="VW077_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         6227 a         4458 c         3937 g         6037 t
ORIGIN      
        1 gttgctcaaa gcaccgcttt gaggttgcag ataaagctga cgtggtttga agagattttc
       61 gaagagtatt agttcaagtg ccagatgtct tcgttatact gagcgattgt acggtcagat
      121 gagaagaatc ctgctttaga aatgttaacg atgactttat ccaaccatgc gtcacggtct
      181 tcgtagtcag caagcatttg ctctttgact ttgatgtagt cttccaagtc aagaagagtc
      241 atgaaccagt ctttgttgat caattcattg taaagacgtt ccaagcgctc tttgtttcca
      301 gctgcaagaa ctgcatcgct aacaatgaag tcaaccaatg gtttgatagc ttcacgagcg
      361 tagaattcgc ttgatttata tgctgctttt gcgtaaaggt cgataacagt ttctgaatct
      421 tcaccaaaga tgtagatgtt ttcgtcacca accaactcag cgatttccac gttagctccg
      481 tccatagtac caagagtcaa agctccgttc aacatgaatt tcatgttacc agtacctgaa
      541 gcttctttag aagcaagtga gatttgttct gagatatcac atgctgggat aaggaaactt
      601 gctgcagtaa cgttgtagtt ttcaaccata actacttgca agtgtggagc tactgctgga
      661 tcgttagcaa taacttctga catgcaaagg attaaatgga taatgtcttg agcgattgtg
      721 taggctggag ctgctttacc accaaagaag attgtgattg gacgagcagg gatgttacca
      781 gctttgatgt caaggtattt gtggattacg tacaaagcgt tcatttgttg gcgtttgtac
      841 tcgtgaagac gtttgatttg gatatcaaag atagaatttg gattgatttc cacaccttgg
      901 tgttctttca agtgacgagc caatttacgt ttgttgtgag ccttgatgct ttccaatttt
      961 tctttgacag ctgctttgtc ttcataagac aaaagttttt caagctcatc tgcttcatgg
     1021 tgccaaccat ctccaagaat ctcatccaag tagtgagaca atcttgggtt agcatgcata
     1081 agccaacgac ggaaagtgat accgtttgtt ttgttgttga acttttctgg gtaaaggtcg
     1141 tagaaggctt tcaactcaga atttttcaag atttcagtat gaagtgctgc aaccccgtta
     1201 acactgtatc cgtagtggat atccatgtga gccatgtgaa cacgtccgct ctcatcgatg
     1261 atttgaacag ctggatcttt gtactctgcc ttcacacgac ggtccaattc ttcgatgatt
     1321 ggtaccaagt gaggaaccac ttcttgcaag aattcaagag gccatttttc aagcgcttca
     1381 gcaaggattg tgtggttagt gtaggcagtc atgctacgaa caattgagat tgcttcgtca
     1441 agctcgatac cacgtgcagt caaaagacga atcaattcag gaatcaccat tgatgggtga
     1501 gtatcgttga tttggacaac tgcgtagtca gcaaggtcat gcaagttgct tcctttttcg
     1561 attgcttcgt cgatgatcaa ttgcgcaccg tttgaaacca tgaagtattg ttggaagata
     1621 cggagcaatt caccttgacg gtcactatca tctgggtaaa ggaagagagt taagttgcga
     1681 gcgatatctg tcttgtcaaa gttgatacca tctttaataa tagaagaatc aactgaatcc
     1741 aagtcaaaca aacgcaagcg gtttttagtc gctgtttcat aaccagtaac atcaatatcg
     1801 taaagagttg atgtcaaagt aaagtctgca aatggtactt ggtagctacg gcttgagcga
     1861 accaaccagt tttgctctgt caaccatgca tttggaattg tttcttgttg gttgttttta
     1921 agaacttgtt ggaaaagacc aaagtggtag ttaagaccaa caccgtcacc attcaaacca
     1981 agagtagcaa ttgagtcgat aaagcaggca gccaaacgtc ccaaaccacc attaccaaga
     2041 gatggttcca attcaacttc ttcaacttcg atcaagtctt tacctgcagc tgcaagttct
     2101 tttttaacat cgtcgtaaag accaaggtta atcaagttgt ttgacaagag tttaccaatc
     2161 aagaactcag ctgagatgta gtaaactttt ttcttaccag tgttgactgg tttttggctg
     2221 cttgcaagct tgctgtagtt aagaagagca aggtaaagct cttcattgct acattctgca
     2281 atggttttat tgtaacgatt ttgtacaaat tcttgtagtg ataacatgtt tagttgtctc
     2341 ctgattattg tcttatttct ttaaatctac caaattttca ttaattcggc gataaattgt
     2401 tgtcaagtca agcaaacctt cctcgacagc tggtgtcaat tgatcttcag tcatacgcca
     2461 agaccagttt ccaccaaggg tagatgggaa gttcatacga gctgcctcat ccaattctag
     2521 taaatcttgc atagttgcaa ttgccataaa gctaactgat gaaaatactg tacgaagcat
     2581 agcgtgtacc actgtttcgt attctttacg gttcgtgtaa cgagccatgt actcacgagt
     2641 cgcatcatca atctcgttac ggtaccaacc aagaaccgta ttgttatcgt gtgttcctgt
     2701 gtacataact gagttagcag gtgccaagtg tgggctatca atgctttcgt cttctgggtt
     2761 gaaggcaaat tgaagaatct tcattcctgg gaagccagta cgttcacgca attcgatcac
     2821 ttcatctgtc atgaagccaa ggtcttctgc gatgatgttt agctcaccaa gttcttcctt
     2881 aacggctgca aaaagcttgt agcctggacc tttcacccac tcaccaggtg ctgctgtatc
     2941 ggaaccagca gggatttccc agtaagattc gaagccacgg aagtggtcga tacgaacgat
     3001 atcgtagatt ttgaagcttt cacgcaagcg ttcaatccac catttgtagc cgtctttgtc
     3061 cattgcttcc cagtcataga ttgggttacc ccaaagctga ccagttacag aaaactcatc
     3121 tggtgggcat cctgcgatac aagtagcctt accattgaca tctgttttga agagatgtgg
     3181 atttgcccac atatcacttg aatcttccgc tacgtagatt ggcatgtccc caacgatttc
     3241 gatgtggttg tcgttagcgt aagctttcaa tttcaaccat tgttggaaga agaagtattg
     3301 agtcacacgg tggtaaacca acttgtctgc caattgctca cgatagcttt caagtgctga
     3361 agctttacga gcacgagcat ctgcatctgg ccattcagtc caagcaagat tgtcaaaata
     3421 ctctttgata gccatatact cagcaaagag ctcaagccat gattggttgt cttgagcaaa
     3481 tttctcaaaa tctttaacat ctccgacttc aaagaaacgt ttcaccgctt tttctaaaag
     3541 aggacgacgt gcatagtaga ttttagcata gtcaacttca gacgcatcgc taccaaagtc
     3601 aactccttca aggtcacttg cttccaacaa accttgctcc accaagatat ctaaatcgat
     3661 aaaatgagtg tttcctgcga aggctgagaa agattggtaa ggagaatccc cgtaactagt
     3721 tgctcctaat ggaaggattt gccagtaacg ttgttttgta cggaccaaga aatcaacgaa
     3781 gtcgtaagca ctttgaccaa atgatccgat tccgtaagct cctggaagag aagagatgtg
     3841 catcaacaca ccactttgac gttttttcat aatagcacct cgtgtgttaa aataatggaa
     3901 cgttgcgtat tttgcagacg caaacgtttg cgttacttat aagtatactc cttttcaagc
     3961 ataattgcaa gcgtttttaa aaaacttttt tgattatttt taaggagact aactcatttt
     4021 agcaaagata acttaaaact atctaatcct aaggtttgac tctcacgaga cagtctacaa
     4081 agtacaaaac ctccttaggg agcttgaatt tatatgtaac aaagcacaaa cgtccataga
     4141 aaatagatag ggattagaac cagggaggta gcccctcctg gtttccctct ttagacatat
     4201 tccatatatt tttattaaaa atactgtatt cttatctatt tcatactttt cttctattaa
     4261 aatgcaaaat tatgctatca aatcaagctt aaaaattttt taaaattttt acaaaaaata
     4321 cttgcaaccg ttttctattt gtgctatact aagctcataa aggaaaacgt ttgcgtttcc
     4381 ttatcaataa aacttgctat tctttgggag gaatacacta tgtcatctaa atttatgaag
     4441 agcgctgcgg tgcttggaac tgttacactt gctagcttgc ttttggtagc ttgcggaagc
     4501 aaaactgctg ataagcctgc tgattctggt tcatctgaag tcaaagaact cactgtatat
     4561 gtagacgagg gatataagag ctatattgaa gaggttgcta aagcttatga aaaagaagct
     4621 ggagtaaaag tcactcttaa aactggtgat gctctaggag gtcttgataa actttctctt
     4681 gacaaccaat ctggtaatgt ccctgatgtt atgatggctc catacgaccg tgtaggtagc
     4741 cttggttctg acggacaact ttcagaagtg aaattgagcg atggtgctaa aacagacgac
     4801 acaactaaat ctcttgtaac agctgctaat ggtaaagttt acggtgctcc tgccgttatc
     4861 gagtcacttg ttatgtacta caacaaagac ttggtgaaag atgctccaaa aacatttgct
     4921 gacttggaaa accttgctaa agatagcaaa tacgcattcg ctggtgaaga tggtaaaact
     4981 actgccttcc tagctgactg gacaaacttc tactatacat atggacttct tgccggtaac
     5041 ggtgcttacg tctttggcca aaacggtaaa gacgctaaag acatcggtct tgcaaacgac
     5101 ggttctatcg taggtatcaa ctacgctaaa tcttggtacg aaaaatggcc taaaggtatg
     5161 caagatacag aaggtgctgg aaacttaatc caaactcaat tccaagaagg taaaacagct
     5221 gctatcatcg acggaccttg gaaagctcaa gcctttaaag atgctaaagt aaactacgga
     5281 gttgcaacta tcccaactct tccaaatgga aaagaatatg ctgcattcgg tggtggtaaa
     5341 gcttgggtca ttcctcaagc cgttaagaac cttgaagctt ctcaaaaatt tgtagacttc
     5401 cttgttgcaa ctgaacaaca aaaagtatta tatgataaga ctaacgaaat cccagctaat
     5461 actgaggctc gttcatacgc tgaaggtaaa aacgatgagt tgacaacagc tgttatcaaa
     5521 cagttcaaga acactcaacc actgccaaac atctctcaaa tgtctgcagt ttgggatcca
     5581 gcgaaaaata tgctctttga tgctgtaagt ggtcaaaaag atgctaaaac agctgctaac
     5641 gatgctgtaa cattgatcaa agaaacaatc aaacaaaaat ttggtgaata aaaaatttgt
     5701 tcaagggggg tggaaatcaa atcccccttt gaatttatca atagagacac aaataattta
     5761 gctttcttat aaaaaagtag tatcctatga aaggagttaa tatggaaaag caacaaccta
     5821 gtaaagcagc cctgctgtct atcattcctg ggttaggaca gatttacaat aaacaaaaag
     5881 ccaaaggttt tatcttcctt ggtgtaacca tcgtatttgt cctttacttc ctagcacttg
     5941 caacccctga attgagcaac ctcatcactc ttggtgacaa accaggtcgt gataattccc
     6001 tctttatgct gattcgtggt gccttccatc taatctttgt aatcgtttat gtactctttt
     6061 atttctcaaa tatcaaagat gcacatacga ttgcaaaacg cattaacaat ggaattccag
     6121 ttccacgcac actcaaagac atgatcaaag ggatttatga aaatggcttc ccttacctct
     6181 tgatcattcc atcttatgtt gccatgacct tcgcgattat cttcccagtt atcgtaacct
     6241 tgatgatcgc ctttaccaac tacgacttcc aacacttgcc accaaacaag ttgttggact
     6301 gggttggttt gaccaacttt acaaacattt ggagcttgag taccttccgt tctgcctttg
     6361 gttctgttct ttcttggact atcatttggg ctttggcagc ttctacttta caaatcgtaa
     6421 ttggtatctt cacagctatc attgccaacc aaccatttat caaaggaaaa cgtatctttg
     6481 gtgttatttt ccttcttcct tgggctgtcc cagccttcat cactatcttg acattctcaa
     6541 acatgtttaa cgatagtgtc ggtgctatca acactcaagt attgccaatc ttggctaaat
     6601 tccttccttt ccttgatgga gctcttattc cttggaaaac agacccaact tggactaaga
     6661 ttgccttgat tatgatgcaa ggttggctcg gattcccata catctacgtt ttgactttgg
     6721 gtatcttgca atctattcct aacgaccttt acgaagcagc ttatattgac ggtgccaacg
     6781 cttggcaaaa attccgcaac atcactttcc caatgatttt ggctgttgcg gcacctactt
     6841 tgattagcca atacaccttc aactttaaca acttctctat catgtacctc ttcaatggtg
     6901 gaggacctgg tagtgtcgga ggtggagctg gttcaaccga tatcttgatc tcatggatct
     6961 accgtttgac aacaggtaca tctcctcaat actcaatggc ggcagctgtt accttgatta
     7021 tctctatcat tgtcatctca atctctatga tcgcattcaa gaaactacac gcatttgata
     7081 tggaggacgt ctaagatgaa taactcaatt aaactcaaac gtagactgac tcaaagcctt
     7141 acttaccttt acctgattgg tctatcaatt gtaattatct atccactgtt gattaccatc
     7201 atgtcagcct ttaaagcagg taacgtctca gcctttaaac tagatactaa tatcgacctc
     7261 aattttgata actttaaagg cctcttcact gaaaccttgt acggtacttg gtacctcaac
     7321 actttgatta tcgccttaat taccatggct gttcaaacaa gtatcatcgt acttgctggt
     7381 tatgcttaca gccgttacaa cttcttggct cgtaaacaaa gtttggtctt cttcttgatc
     7441 atccaaatgg tgccaactat ggccgctttg acagccttct tcgttatggc gcttatgttg
     7501 aacgccctta accacaactg gttcctcatc ttcctctacg ttggtggtgg tatcccgatg
     7561 aatgcttggc tcatgaaagg ctacttcgat acagtgccaa tgtctttaga cgaatctgca
     7621 aaactagacg gtgcaggaca cttccgccgc ttctggcaaa ttgttctacc acttgttcgc
     7681 ccaatggttg ccgtacaagc tctctgggcc ttcatgggac ctttcgggga ctacatcctc
     7741 tctagtttct tgcttcgtga gaaagaatac tttactgttg ccgtaggtct ccaaaccttc
     7801 gttaacaatg cgaaaaactt gaagattgcc tacttctcag caggtgctat cctcatcgcc
     7861 cttccaatct gtattctctt cttcttccta caaaagaact ttgtttcagg acttacaagt
     7921 ggtggcgaca agggataatt tatccccgcc accctttttc attttatact cttcgaaaat
     7981 ctcttcaagc cacgtcagct ttatctccaa cctcaaagtt gtgctttgag caacctgcag
     8041 ctagcttcct ggtttgcact ttgattttca ttgactatta gcgattgtca ctgtaaataa
     8101 tatccttgta gcaagcaatt taccccctag acttgaaata aagcgcattt ctctatataa
     8161 taatactcat atagaaaaca ccttttagaa agatacctat gcttccatat ccattttcct
     8221 atttttcaag tatttggggg tttcgtaagc ccctgtccaa acgtttcgag ctcaactggt
     8281 ttcaacttct ctttaccagt atcttcctta tcagcttgtc tatggtaccc attgctatcc
     8341 aaaacagctc ccaggagacc tatccgctag aaacttttat cgataatgtc tatgaacctc
     8401 tgacaaatga ggttgtccag gatctctctg aacatgctac aattgtcgat ggcacattaa
     8461 cttatactgg aacagctagt caagcccctt ctgttgtgat tggtccaagt caaatcaagg
     8521 aattacctaa ggacttgcaa ctgcatttcg atacaaatga gctagtcatc agcaaggaaa
     8581 gcaaggaact gacccgcatc tcttaccgag ccattcagac tgagagtttc aaaagcaaag
     8641 acagcttgac ccaagcaatt tctaaagact ggtaccaaca aaatcgtgtc tatatcagcc
     8701 tcttcctagt tctcggtgcg agcttcctct ttggtttgaa tttctttatc gtctctctag
     8761 gagctagtct tctcctttat atcactaaga aatcacgcct cttttcattt agaaccttta
     8821 aagagtgcta tcactttatc ttgaactgtt taggattgcc gactctgatt acacttattt
     8881 tgggattatt tggccaaaat atgacaaccc tgattactgt acaaaatatt ctttttgttc
     8941 tgtatctggt cactatcttt tataaaacac atttccgtga tccaaattac cataaatagg
     9001 agatttttat gcccgttacg attaaagacg tggccaaggc tgctggtgtt tcgccttcaa
     9061 ccgtaacccg tgttattcaa aataaatcaa ccattagcga cgaaacaaaa aaacgcgttc
     9121 gcaaagctat gaaggaactc aactaccacc caaacctcaa cgctcgtagc ttggtaagca
     9181 gctatactca ggttatcgga ttagttcttc ctgatgactc agacgccttc taccagaatc
     9241 ctttctttcc atcggttcta cgtggcatct ctcaagtcgc atctgaaaac cactatgcca
     9301 ttcagatagc aacagggaaa gatgagaagg agcgtctcaa cgctatttca caaatggtct
     9361 acggcaagcg tgtagatggg ctaatctttc tctatgccca agaagaagac cctctcgtaa
     9421 aactcgtcgc agaagaacag ttccccttcc ttatcttagg taaatctcta tctcctttca
     9481 tcccacttgt cgacaacgac aatgttcaag ctggttttga tgcgactgaa tatttcatca
     9541 aaaaaggctg caaacgcatt gcctttatcg gaggaagtaa aaagctcttc gtgaccaaag
     9601 accgtttaac aggctatgaa caggcgctta aacattacaa acttaccact gacaacaatc
     9661 gcatctactt tgccgacgag tttctggaag aaaagggcta taaatttagc aagcgattat
     9721 tcaagcacga tccacaaatt gatgctatca tcacaaccga tagcctccta gctgaaggtg
     9781 tttgtaacta tattgccaaa caccagctgg atgtccctgt tctcagcttt gactcggtta
     9841 atcccaagct caacttggca gcctatgtcg atatcaatag tttagagctt ggtcgtgttt
     9901 cccttgaaac tattctccag attattaatg ataataaaaa caataaacaa atttgttacc
     9961 gtcaattgat cgcccacaaa attatcgaaa aataagagac tgggcaaaaa gtcgttaaaa
    10021 gcaaaaacgc atactatcag gtattgaaaa aacttgatac tatgcgtttt attgtgggaa
    10081 gatttacttc cttttctact gaaattgagt tgttacccaa gatcttttta tactcaatga
    10141 aaatcaaagt gcaaactagg aagctagcca caggttgctc aaaacactgt tttgaggttg
    10201 tggatagaac tgacgaagtc agctcaaaac atggttttga ggttgtagat gaaactgacg
    10261 gagtcagctc aaaacatggt tttgaggttg tagatgaaac tgacgaagtc agctcaaaac
    10321 atggttttga ggttgcagat agaactgacg aagtcagtaa catacatacg gtaaggcgac
    10381 gctgacgtgg tttgaagaga ttttcgaaga gtattaatca ctaattatct atctcaacaa
    10441 atcttcctag aatatgaaca ttttccgaga cagagacaaa ggagcttgga tccacttgtg
    10501 tcataatctg tttaaattca ttaaactctg cacgtgtaat gacagtgatt aaaactgcct
    10561 ttctctcgtg attataggtt ccttctgcat catggatcat ggttgctccg cggtgcaatt
    10621 ttttatggat tttttcaatt accttctctg gatgatttgt cacaatcatg gcctgcatac
    10681 gcttttgctt agtaaagact gcgtctgtca cacggctaga gacaaagatg gtaatcatag
    10741 aataaagagc gtatttccaa ccaaaggtca aacctgctat cagcatgata gttccattta
    10801 ccaagaaaga aatactacca acattcttac ccgttttctt gcgaatagtc aggctgacga
    10861 tatccgtccc accactggag atattgtttc gaagagcaaa accaatcccc aaacccataa
    10921 caacaccccc aaaaagggca ttgataatgg gatcctccgt caaggttgcc acagggacaa
    10981 actggataaa gaaggaactc atagataccg tgataaaggt aaagacggtg aacttatggc
    11041 caatctgata ccaagctaag accatcaaag ggaagttaat ggcgtagaag gttagcgaaa
    11101 tcggaatatg aaaaccaaac cagtgattac tcaaggcaga gataatctgt gccagacctg
    11161 ttgcaccact cgaatacaca tgccctggtt ggaaaaagaa attaactgct actgctgata
    11221 aaaaaccata gaccagagag gccgaaatct tctcatcata cttttctcga gagatacttt
    11281 gtaagacacg taaaattttt atctgataag caaagcggcg cagataatag cgccaccgct
    11341 taattcgttt tgtttgtttc atcttcttct acttgtaagc tgagttcctc tagttgtttg
    11401 agagcgactg ttgatggagc ttgtgtcatt gggtcagttg ccttgttgtt cttaggaaag
    11461 gcaatgactt cacggatatt ttcttctcca gcaagcaaca tgacaaaacg gtcaagcccg
    11521 atagccaaac caccgtgtgg tgggaaacca tagtccatgg cttcaagaag gaaaccaaac
    11581 tggtcattgg cttcttcagt tgagaaacca agagccttga acatgcgttc ttgaaggtct
    11641 ttttggttga tacgaaggct accaccacca agctcataac cgttcaagac gatatcgtaa
    11701 gcaatggcac gaaccttagc caaatcacct tctaattcat gagcagtctc ttcctgtgga
    11761 agtgtgaaag gatggtgggc gctcatgtag cggccttctt cttcagacca ttcaaacatc
    11821 ggccagtcaa ccacccaaag gaagttgaac ttatcattat caatcaagcc aagctcttta
    11881 gcaatacgtc cacgaagggc acccagtgtt gcattagcca cttcaagcgt atccgccaca
    11941 aagagaacca agtccttatc ttcaagaaca agcgctgttg tcaattcttc ttggatacca
    12001 gtcaagaact tggcaactgg tccgtttaat tctccatcaa ccaccttgac ccaagcaaga
    12061 cctttggcac catactgttt ggctacttcc gtcatcttgt cgatgtcttt acgtgaatag
    12121 ttgtccgcag ctcctgtgac cacaatcgct tttacagcag gtgcttctga aaagacttta
    12181 aagtctacac ctcggaccac ttctgtcaag tcctgaagca acatgtcaaa acgagtatct
    12241 ggcttgtcag aaccgtaaag agccatagca tcatcgtatt tcatacgagg gaatggtagc
    12301 gttacttcga tgccttttgt ttccttcatc acgcgcgcga tcaagctttc tgtaatatct
    12361 tggatttctt gctcagtaag gaaggacgtt tccaagtcga cctgagtaaa ttcaggctgg
    12421 cggtctccac gcaagtcctc gtcacggaaa catttaacga tttggtagta acggtcaaaa
    12481 ccagcattca tcaagagctg tttcgtgatt tgtggacttt gaggaagagc gtaaaaatgc
    12541 cccttattaa cacgagacgg cactaaataa tcacgcgccc cttcaggcgt tgacttagaa
    12601 aggaatggtg tctccacgtc gataaactcc aactcatcca agtagttgcg gatagagtgg
    12661 gtcaccttgg cacgaagttt aagattttcc aacatttctg gacgacgaag gtcaaggtaa
    12721 cggtaacgca aacgtgtatc gtcatttgcc tcaatgccat ccttaatctc aaatggtgtt
    12781 gtcttagctg tgttaagcac aataagagct gtcacgttta actcaaccgc accagttggc
    12841 aacttatcat tggcttgctc acgcgcagcg acctgaccag tcacctcaat aacaaattcg
    12901 ctacgaaggc tttcagctgt tgccataacc tctgcagata ctttttcagg gttgataacc
    12961 aactgcatga ttccttcacg gtcacgaaga tcgataaaga tcaaaccacc aaggtcacga
    13021 cgacggccaa cccatccttt caaggttatt tcttgtccga tgtgttcctc acgaacacga
    13081 ccagcataca tactacgttt cattatttct ctcctctttt attctgttac tattttacca
    13141 taaaagcgca ggctcttcat gaaaatcatc agaaaagttt gccagtcttt aaaagtcagg
    13201 tgaaagccct aaaaattagc gctaatactc ttcgaaaatc tcttcaagcc acgtcagcgt
    13261 cgccttaccg taggtatggt tactgacttc gtcagtttca tctacaacct caaaaccatg
    13321 ttttgagctg acttcgtcag ttctatccac aacctcaaaa cagtgttttg agcaacctgc
    13381 ggctagcttc ctagtttgct ctttgatttt cattgagtat aatacaaaaa tccgatgaac
    13441 ttcaccggac tcttttattt tgaatttttg cctgctttac gcttttcagc gatttcggct
    13501 gcctttcgag gcaagacaat ttccgttatg taagccgtcc caaaacgcag tacacctgca
    13561 ataggagcaa agacaactgc tagatagtta tagaagaaat cgcctttgaa ggcataagct
    13621 agcgctccaa tgatgaaaaa tagaacgact gcctgaatca ctgctaataa aattactcgt
    13681 ttcatgtgac ctcctgactc tattatagca tgagaatcat caaaaagccg actaaattat
    13741 tcaaagcgtg aagagaaata ctgtagacca gaccttttct gctaacgtaa gccaaaccca
    13801 aactaaaacc aaggctaaaa tagacaaaaa attgttgcac atcacctgga aaatgaatca
    13861 aggcaaatag aagactagat accagaagaa aaatcagggt tcgtttacta ttgtcctgct
    13921 taggaaagag atagcgtgct aacatccctc taaaaacaat ctcttccgtc aaaggagcaa
    13981 aaataaccac agcaaagaat gagaaaagtg gttgagacaa ggtcaagtct gtcgctattt
    14041 gctgatttac tgaaggatca tctggcaaga agaattgaac gaccagagat aagaaccaaa
    14101 ccaagacagg aagccaaata aatcgattaa agccgctctt ctcaatatga acaggagcct
    14161 tctgatacca tttgtaaatg ccgtacacat atactccagc caaggccaca tagagtagag
    14221 taacagcata gggtgaagcg cctaaagcaa gcgacgcagt cgcgagcccc tgaataaagc
    14281 catagataaa taaaaaggat agaagggcta gaagaatcca gccaaggttt ttaagtaatt
    14341 tcatagataa ctcctttatt tgaaataacg ttttaccata ggtaactgca tcacattgat
    14401 ataaacatgg atggctccta caagcaagaa agctagtaac tgaatctctc ctgtcaagaa
    14461 agaaatgata ataagaaaaa tatataaggc tggtaagaca tattggtgta attggaataa
    14521 aattcgaaaa ctctgttcca aattagcctg acgctcccct tcatcataag aatttatata
    14581 gttcaagaca tcctttggtg tagcgaaaaa ttccaaatca aactgacgaa caatcgcaat
    14641 ggttttaaaa agagattttt gagcgactaa gaataccaca aagagtaaga aagaaaggaa
    14701 aaatgtttga gggtttgtat gcaatataat cacctcactt aatgaaataa aaatagccaa
    14761 tggaatcgct acacctataa tattaaaagc aatggttcca aactcaagat tccgatacat
    14821 ttgcacataa taggtttcat tcagatcgtc atccatttcc tcttgataca aggaatgaaa
    14881 ttttctgctt ttctttaaga aattgaaagt caaaaacata ctaatgaaac ctatcagtaa
    14941 acaaatagct gatatccatg gcatcaaggc ttttacatct aaaataattt cgtgggattc
    15001 gacacgtgcc ttaaacatcc ctacaaacat gcccaagaac ccccccaaga caatagacat
    15061 caaaaataac aatctacgtt tctttttcat attcattctc ctttttcact tgctagattt
    15121 ttggatttct tttcaatcca ttcagttact gggatgagag caaagtagac ccaaacaaat
    15181 tggtcgcttt gatagggatt aaaccagctt aggtccatcc caatcagtag aaatacgctg
    15241 actaataaag ctatgaccac tacataataa atcactttat acttgttcat cactcgtcct
    15301 cctccaaacg aaataccgat tcgactgttt cgttgaaaat ttgagatatt ttcagggcaa
    15361 tgataatgga tggggtgtac tcatcccgtt ctagtaggct aatggtctgt ctggaaaccc
    15421 ctgccagttt ggctaggtcg gtttgattga gaccatcgcg agctcgaagc tcttttagac
    15481 gattttttag ttgcacgtta cacacctact ctccgtcaaa ttcaacggtt tggatatctt
    15541 caatacgttg caacttgaat ttttcttttc ccgtattatc aacactacgt agtttgaccc
    15601 attcctcatc aacatccaca acttcccagt tatctggccc aatatacact cccgttataa
    15661 ttggttcctt tccaatcatt tcttgtaata atctcgacat ttctgcgttt cctttctctt
    15721 ttcgctcaag ttttttgatt ttattctcta gtttcttgat ttttttagaa ttattagaat
    15781 aaaagaaaat cataaatagt ataaatccta gtacccacat tataactcct ttctgcttcc
    15841 tatttcttaa cttgaattca ttgtaacata tctttttctt tttgacaatt atagttgtca
    15901 aaaagtttat gatttttgtc attttgcaaa agaaaaaggt caggagtaag ttcctgacca
    15961 ctttatctat cattaatact cttctaaaat ctcttcaaac cacgtcagct tcaccttgcc
    16021 gtaggtatgg ttactgactt cgtcagtttc atctacaacc tcaaaaccat gttttgagct
    16081 gacttcgtca gttctatcca taacttcaaa acagtgtttt gagcaatctg cggctagctt
    16141 cctagtttgc tctttgattt ttattgagta taaaatccta gtttttcaaa gatttctgaa
    16201 aagttttggc tgattgtctc aagtgacact tgcacttctt ctcgggtttg gttgttcttg
    16261 accgtcactt gtccgctttc gacttcgctc tctcccaggg tgatgagggt cttagtcgca
    16321 aagacatcgg ctgacttgaa ctgagctttg agcttacggt tgaggtaatc acgctctgct
    16381 ttgaaacctt gttggcgaag ggcttggact agttctaggg ctttgacatt tgctccatca
    16441 cccaagactg cgatatagac atctaaggca ttttcgatag ggagggccac gccttgtttt
    16501 tcaaggatga gaagcagacg ctctacacca agcccaaaac caaatccagc agtttcaggg
    16561 cctccaaagt aagcaaccaa accatcgtag cgaccacccg cacagactgt caagtcattg
    16621 ccctcaatct ctgtgataaa ctcgaaaatg gtgtggttgt agtagtccag accacgcacc
    16681 atattggtat cgatgatgta atctactcca agattttcca acatctgacg cacagcatca
    16741 aaatgagctt ggctttcttc atcaagaaag tccaagatag acggcgcatt ctctactgcc
    16801 accttgtctt ctttttcctt agagtccaag acacgaagag gattttcctc caagcgacgt
    16861 tggctatcct tagacaaggt ctccttgagc ggtgtcaaat agtcaatcaa ggcttggcgg
    16921 taggctgcac ggctctcagg atttccaaga gtgttgaggt gcaatttgac accttgaata
    16981 ccgatttcct tcaaaaaatg ggctgccata acgattgttt ccacatcggt agctggattg
    17041 ctagagccaa aacactcaac accaatctgg tggaattggc gcaagcgccc tgcctgtgga
    17101 cgctcataac ggaacatagg tcccatatag tagaacttgc ttggcttttg cacttctggg
    17161 gcgaagagtt tattttccac ataggaacgg acaacgggcg cagttccttc tggacggagg
    17221 gtaatatgac ggtcaccctt gtcataaaaa tcgtacattt ccttggttac gatatccgtt
    17281 gtatctccga cagagcggct gataacctcg taatgctcaa aaataggcgt gcgcacttct
    17341 gcatagttat agcgcttgaa aatctcacgg gcaaagccct caacgtactg ccacttagca
    17401 gactcagcag gtaaaatatc ctgcgttcct tttggttttt gtaatttcat agggaatcct
    17461 ctttaaactt aatagtctta ttttaccata aatagaggga ttaaaacagt aagaaaaaaa
    17521 ttaggattta gatatcattt ttgagattaa gaattgttaa aaaaatagct agcaaggaaa
    17581 gaccaacaaa tagcatccaa gtcaactgca tgttccatac ggctactagt gaaaaacaag
    17641 ctgttcccac aggtatggat aaggtaaaca atagacctaa aaaattacta gtacgagcta
    17701 gaacctctgg agctagattt ttcatgagca tggcactaat ctttggttga actttaccag
    17761 acacatacag agtaaagaag agaaatagca aaccaagcac gacttgattg aataaattag
    17821 ccaaaccaac tagactaagt cctacggtcg cccacatcat caatctaggc aaggactgct
    17881 tcccaaaata agcattgccc gtaaggctac tgatgatgat tgctactaaa acacagaatt
    17941 gattgataaa tagtgcctct gtataagaaa aattcaagag agaatggctc aaaaagaaga
    18001 tattataaat tccacccaaa gcgccaccca aggaattaat aagcaagaca gcaaagagca
    18061 taaaaccaaa gtttttctgt ccacttttaa gaaaaacgag acgtaaattt cggtaaattg
    18121 ttaggaactg gtctttgata gaaagcttct cattttttaa gttttcacca tcagcagatg
    18181 acattgacag gctcaatttg ctttttccta aaaagaggat agtggctgat actaggaaaa
    18241 agcaggcatt gattcccgca acgagagaaa aattgttgac cgatagagct aagagccaga
    18301 ctccgaaagc ttgaccacca atagctgaaa tataggtgat gaactgtgaa aaagaataag
    18361 cctccatcaa atcatcttca gctacttttt ccttaataag aggcatacgc aggccacctg
    18421 caaaatcact gatgatatca ctaatgacat tgatcaaaca caggctagaa aaggcaaaga
    18481 gactagcttg ctgaacaact agggctgcta gaaaaaatag aaccgcctga aacaaaccgc
    18541 tatagaccat ccatttgacc ttgtccctcg tgtaatctgc ccgaatccct acaaaaactg
    18601 taaagagggt cggaagaatc atgacaatat tcgccatagc aacagcaaaa gatgcttgtg
    18661 acaaggtcga tgcatagacg ataaagacca ggttgaaaat cgaagcacca aaagcattga
    18721 agaagcgtga taaaatcaaa attctataag ctggatttct aaacaatagt ttcatagata
    18781 atctcccttc ttgttcgaac ctctatatga agtatgacta tagtatagca cttagaaatt
    18841 tgagagggag aactcttgaa tatatgcaac aaataaaatg agcaaaaaga aacgatagaa
    18901 cttttgtaat gacctgagag agggcaatag ttctatcgct tcaaattttt tcttggtttg
    18961 cagatattca acattcagtc gtatttctat agtattcggc agatttatta cagccaagca
    19021 tctcaaaaat acggacagca tcctccatct ttttctggcc ttccttgact ctaccttgct
    19081 tgctatcaag gagaccttct gcccacagat aaacaattcg gaaataggtc tcattttcct
    19141 tgtagaaatg ctcttcgata acacgtttaa aataataggc attggtaaat tcttcacact
    19201 caatactagc taaaaagcca ttcaatagta tagtatgaaa aaggtttcga ttgccagaca
    19261 tttccattag aaaatcagat ttacgtacca tttctcgtac atatctagta aaaagagaaa
    19321 cagataaaaa tggagaactg actgaaaata aattgagttc atagattccc cagatctcgg
    19381 tagaaaacaa ataatcatga aggacttttc cttcctctgc tgttaactct accctttcat
    19441 ccatgctctt catataagac ttgataataa tggcatttag aatatgtttc tgtttgttgt
    19501 gagaatgggc atgcttttga tactccctgc gatataagtc ctcaagaggt gctatattct
    19561 ttggttccaa gacatctgta atttcttttc tcaactcaga atctgtatca tactggaaac
    19621 ctctcgccag aaagaggatc tcctccacac tggcagatat attttccaga gcaaatagaa
    19681 acttttccac cgaaagctca ctctgacctg tttcaaaacg ggacaacata gacggcgaaa
    19741 attgtcctcc ggttgcttgt ctcagtgaga tatttcttga ctctcgtaat tgtctaaaga
    19801 cttttccaat ctgctccata gacttcccct tgattccgta ttttcttcat tttatcatat
    19861 ttttcagaaa attcatcaaa aacttgccaa attgtcagaa ttatgagaaa atagaggata
    19921 tttatcacgt ggagggactg ctatgagaga cgatatcaaa atcaatgacc gtgctttggc
    19981 cttgcaagac caaattatcg aaaaactaga gaaagttttt gatacagatg tggaattgga
    20041 tgtttacaat ctaggtctga tttatgaaat caatctggat gaaacggggc tctgcaagat
    20101 tgtcatgacc ttcaccgata ctgcctgtga ttgcgccgaa agcctgccta ttgaaattgt
    20161 ggcaggtctg aaacaaatcg agggtatcaa agatatcaag gttgaagtta cctggtcgcc
    20221 tgcttggaaa atcacacgaa tcagtcgcta tggccgtatt gcccttggac taccacctcg
    20281 ttaagcaggc caatcacttt taaagatgaa aaaagcaagc cgaaaatggc ttgctttttg
    20341 atactcaatg aaaatcaaag ggcaaactag aaaactagcc gcaggttgct caaaacactg
    20401 ttttgaagtt atggatagaa ctgacgaagt cagctcaaaa cactgttttg aggttatgga
    20461 tagaactgac gaagtcagcc caaaacactg ttttgaggtt gtggatagaa ctgacgaagt
    20521 cagcccaaaa cactgttttg aggttgtaga tagaactgac gaagtcagct caaaacactg
    20581 ttttgaagtt atggatagaa ctgacgaagt cagctcaaaa cactgttttg aggttgtaga
    20641 tagaactgac gaagtcagc
//
LOCUS       DAVKGU010000026        20339 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKGU010000026 DAVKGU010000000
VERSION     DAVKGU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.026"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..3488)
                     /gene="mfd"
                     /locus_tag="VW077_000941"
     CDS             complement(<1..3488)
                     /gene="mfd"
                     /locus_tag="VW077_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9454981.1"
                     /translation="MVTLLDLLSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKRKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNK
                     KDYEILEGLLIFGESL"
     gene            complement(3489..4058)
                     /locus_tag="VW077_000942"
     CDS             complement(3489..4058)
                     /locus_tag="VW077_000942"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9454982.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            complement(4129..5244)
                     /gene="ychF"
                     /locus_tag="VW077_000943"
     CDS             complement(4129..5244)
                     /gene="ychF"
                     /locus_tag="VW077_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9454983.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(5364..6530)
                     /locus_tag="VW077_000944"
     CDS             complement(5364..6530)
                     /locus_tag="VW077_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9454984.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKGGATVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKFKIDKYLL
                     PAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYAVSLQAIP
                     FNPARDVIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLKNGTLDVNKTLNIIKEITGPKTKSSIRVIDLDNKTVLMLRLY
                     KARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFHAFRHTHA
                     SILLNAGLPYKEIQIRLGHAKLSMTMDIYSHLSKDNQKNATSFYEKAIEKLKSS"
     gene            complement(6626..7078)
                     /locus_tag="VW077_000945"
     CDS             complement(6626..7078)
                     /locus_tag="VW077_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9454985.1"
                     /translation="MDKINYFSAEEAILLHDIEVIEKIGGLHGVKDKGRVFSVFEHLQ
                     NDDYYPDFETKLTVLVFNTVQFHMFNDGNKRSSIVFGIKFLTINHLDYLIPFFIETME
                     NIVLWVAMGLIDFNFLKEIISSILYYEELTEKVKEKLVQIALAEENNK"
     gene            complement(7078..8082)
                     /locus_tag="VW077_000946"
     CDS             complement(7078..8082)
                     /locus_tag="VW077_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9454986.1"
                     /translation="MTDLTTSQIERQNILNNSLALQKAEEILKVPGFYFEDTFYFTNS
                     QLATFFEVDIRTIERLVEAHKTELTENGYHTLRGEKLAKFKENAFATDTNVGSKVTQL
                     SISSFRTLLNFAMLLTNSDIAKQVRNTLLDIVINVLTEKTGGHVKFINQRDKNYLSTA
                     LEEDNARKIFTNAIDKYVSGSNFKYGQLTNEIYKAIFKERASEYKKILKLNKNDNARA
                     TMYSEVLIVIASFEKGVAYEIEQKSKSSNQKLTNTDVIEIIQRLANHPMQEPHLMHAR
                     EKMASRDLSFRNAKHEKLAEYIRPISEEDFERFIGEQSKSLSEQIDEHRQVFERLRDK
                     "
     gene            complement(8231..9187)
                     /locus_tag="VW077_000947"
     CDS             complement(8231..9187)
                     /locus_tag="VW077_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9454987.1"
                     /translation="MAKNSPQDLENREYFSKRLNLLMEQKGVRQIDLHNALDIPKSTI
                     TGYVKGKSLPTAGNLQKIADFLNVKKSFLDLRFIDTVSTSTLKELLDNNQREKVFEMF
                     FNKIKEILSFKKDYMNMEYIRGKIAFDQGVLGRVLQSSIINPGIVEIFNGLDLSDISK
                     NNEALLLSKQREAREYAYNNVLTMITNEYITRLINVIEREQALNTSYVHSEISNLINS
                     LNNKIDSYGAVVFEVNEIIEKIGIQDRKILGYCLQRKISSVQNEINDLVHNNTNNSFC
                     VTKNKIFTEKSDIIDTIEFDYYKGIQNRLNDLAEEIREHFSD"
     gene            9344..9577
                     /locus_tag="VW077_000948"
     CDS             9344..9577
                     /locus_tag="VW077_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9454988.1"
                     /translation="MEQWTLKACRVNAGYTLRQVAKKVNKNFQTVSKYEKDSTYIPFE
                     LLKELSAIYQVKLDNIFLGDSTKKIELNKRELQ"
     gene            9667..10293
                     /locus_tag="VW077_000949"
     CDS             9667..10293
                     /locus_tag="VW077_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU9454989.1"
                     /translation="MELVYMDGKKEPYTLSSIVAECAEVKHRHLKILLNKHREDFESF
                     GKVQFKISPSESGQNVRDYILNEQQATLLITYLRNTEPVKEFKKNLVKAFFEMREELS
                     KFRMQRALEKPKRKTLHDSIKTWEAKPKHAHSTMNNLLLKAVTDMNAKQLVKSRGGYN
                     GIDSLTSDELEQYQAFEDMVIAMIGLNMSYQEIKAMAFRNKKTRQRSV"
     gene            10745..11197
                     /locus_tag="VW077_000950"
     CDS             10745..11197
                     /locus_tag="VW077_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454990.1"
                     /translation="MTETTYENLTRRINRISAEMQEIGEKNGLSRLSLLASQTKFIKE
                     ELSRLLWIELPELNDSHKIEAVSKSTTGMFFHPGIFEMDAMRQAFFKRQAMHFFENEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            11194..11394
                     /locus_tag="VW077_000951"
     CDS             11194..11394
                     /locus_tag="VW077_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454991.1"
                     /translation="MTLDLDNMTQAEFDKQMAEIKERNPNIFQLISDFLDRKVTPEEV
                     DEFLKMERAEQVEYIKNYQARV"
     gene            11396..11611
                     /locus_tag="VW077_000952"
     CDS             11396..11611
                     /locus_tag="VW077_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454992.1"
                     /translation="MNELDISNTQAAVLIVILIGLLLYLNHRDRKKSAQIERENQQTI
                     KTPSEDLNPDYGRYIQLAGVNVWGGME"
     gene            11608..11901
                     /locus_tag="VW077_000953"
     CDS             11608..11901
                     /locus_tag="VW077_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454993.1"
                     /translation="MSYTMNLYPDIEKIPDETYFTKEIFLATCLNNTIRIAVARGVLT
                     SQLLDKETKEVVCKTLDTIIENQKAILQGVASGQITFVNKSKGEEGLDESIRE"
     gene            11882..12223
                     /locus_tag="VW077_000954"
     CDS             11882..12223
                     /locus_tag="VW077_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9454994.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            12216..12491
                     /locus_tag="VW077_000955"
     CDS             12216..12491
                     /locus_tag="VW077_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454995.1"
                     /translation="MFSLSRESEQDLAHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGARIIFYLVDDIRRFMGVENG"
     gene            12631..13491
                     /locus_tag="VW077_000956"
     CDS             12631..13491
                     /locus_tag="VW077_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEU9454996.1"
                     /translation="MTIYEARGFQSNLVYPFDKMEPFQYIERFKPLVVPESADPEEYK
                     RTQAPYCLSGKVMPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTTEDFIEAVSSALFGY
                     SYILYPTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKIVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVAVTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTFSS
                     IARAEYRKRG"
     gene            13544..15013
                     /locus_tag="VW077_000957"
     CDS             13544..15013
                     /locus_tag="VW077_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEU9454997.1"
                     /translation="MEQEHPPRTMKELENRIFKAGEEWRAEHTETKVNETTGDVTEKV
                     AMPQTFTVAKILSEIVTFTFISKSNIPDYSLLYIYDLDEGIYTASNDLFNLLCKTFDV
                     RIKPREWPQIKLMVRTLAKIRKPLESANLVPVKNGIIDLRTKELLPFSPKYVITSKIS
                     TAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAINPNHTRNKFAIFYG
                     DGNNGKGTFQRFLINLIGESNISALKPAQFGEKHNLETLVGKVCNIGDEAPNEYLKNP
                     SDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVPFNA
                     DFNGEKEKPWIKNEFLANKEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEDNDYL
                     LSWVKHEYMEKGWHELDVVPVFIVTRSLKHYAEDMGIPKPNVYGAGKETIKHLQQLTP
                     NRYSLRKKRVPSEDFEKLEAWDVDKIKMRTPQHSITKQE"
     gene            15354..15521
                     /locus_tag="VW077_000958"
     CDS             15354..15521
                     /locus_tag="VW077_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9454998.1"
                     /translation="MKVKLFEYTRRWEKEDFEKEINDFMATVEVLDVKFSGTPSSESF
                     NSVAIVLVLYR"
     gene            15604..16134
                     /locus_tag="VW077_000959"
                     /pseudo
     CDS             15604..16134
                     /locus_tag="VW077_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173118.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16205..16704
                     /locus_tag="VW077_000960"
                     /pseudo
     CDS             16205..16704
                     /locus_tag="VW077_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17391..17801
                     /locus_tag="VW077_000961"
     CDS             17391..17801
                     /locus_tag="VW077_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU9454999.1"
                     /translation="MTPEQVKEKLEGVKWINKEIEGLYLELAALESGIIKKQELSNTR
                     VQTSRVNTTENNPISVLKLKEDTLQRIERLNEERMEISRLIDKLANPLERSVLRLFYL
                     NVLDAWQVAEEIDKSKTAVYIIRQKAIEHLANVG"
     gene            17957..18349
                     /locus_tag="VW077_000962"
     CDS             17957..18349
                     /locus_tag="VW077_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9455000.1"
                     /translation="MELDKFKTMMNVRERMTYFLRFQRMAGSENQVTIDEEAWKLVLP
                     YRWDLSGEHEKAIREGLEIFAQDINSIENKRARKYFIIHYCYMRKKTMSECVEMAATS
                     STSYHRYKQIAVLNFARIHQNGELEVYK"
     gene            18403..18561
                     /locus_tag="VW077_000963"
     CDS             18403..18561
                     /locus_tag="VW077_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455001.1"
                     /translation="MIEDFLIDLENIQLEDMEIDFNMDIDIDLNIDFEIDVDLDDLDL
                     TLLQELEA"
     gene            complement(18990..19184)
                     /locus_tag="VW077_000964"
     CDS             complement(18990..19184)
                     /locus_tag="VW077_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9455002.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANCWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(19249..>20339)
                     /gene="dnaN"
                     /locus_tag="VW077_000965"
     CDS             complement(19249..>20339)
                     /gene="dnaN"
                     /locus_tag="VW077_000965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9455003.1"
                     /translation="TTKRAISSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQ
                     KNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGK
                     DSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELK
                     TVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFR
                     SENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKL
                     EIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISF
                     ISAVRPFTLVPADTDEDFMQLITPVRTN"
BASE COUNT         6583 a         3689 c         3849 g         6218 t
ORIGIN      
        1 aataaacttt ctccaaaaat cagcagacct tctaaaattt cataatcttt cttattttgg
       61 acatcaaata caagctccat taatccctta ttctcagcga tgcctgcttt taagttcgtt
      121 acggataaag ctttaaaata atcttgagct aaaaacaatc gttgagtgac tttttcaaat
      181 tgaattgtaa ttttattatc ttttctttta ttatcttttc tttccacacg ttgaacaaag
      241 accttgtcca agtatgattt aaccaaacca atctctaaaa gataggctac tacatctggg
      301 tattctccaa aacggtctat caactcctct tgtaactctt cataattgac acggttgtca
      361 atttgacgaa ttttcttgta aatttcaatc ttatgtcgtt gatcagaaat ataagtatca
      421 ggaagatagg catcaatttg caaaatcaac tcagcattcc ctttggttct tgtgttagcg
      481 ttagcattac cgtttcgttt agcaatagct tcctctaata actgcgaata caattcaaaa
      541 ccaacagaat caatgaaacc agactgggat tttcctaaaa gatttcctgc tccacgaatc
      601 gaaagatctc gcattgcaat cttaaagcca gagcccaatt ctgtaaatcc tttaatcgct
      661 tctaatctct tttcagagac ttcactgatt gatttttctg gacgatacat gagataagca
      721 taagcaatac gattactacg accgactctt cctcttaact gatataaggt tgacaagccc
      781 atatggtccg cattttcaat aaataaagta ttagcatttg gaatgtccac ccctgtctca
      841 ataatagtag tcgtcaccaa gatatcgtat tgtccctcaa taaagtctaa tagagtattt
      901 tccaactgga cttcactcat tcgaccatga acatatccaa tcgaagcctc cggaatcaac
      961 tcctgtaatt ctgaaacctt ctgaacaatt gtgtcaactt tgttgtaaag ataataaact
     1021 tgacctccac gctccatttc acgcaagaca gcatcacgaa tgacactatc attcttttcc
     1081 aaaacatagg tctgaacagg atagcgatta gtcggcggag tttcaataac agataaatct
     1141 ctgattccca gcatagacat atggagggta cgagggattg gcgtagcggt caaggttagg
     1201 acatccactt gtttcttcag ttctttcaaa gtttccttat gcttgacacc aaatcgctgt
     1261 tcctcatcaa taatcatcaa gcccaaatca gcaaacacaa catcttttga caaaacacga
     1321 tgtgttccaa tcaaaatatc gacttgaccg tttttcaatt tttcaagtgt tgcagtctgc
     1381 tcttttttac ttctaaagcg actcaacaca tcaatattaa ctgcaaaatt ttggaatcgt
     1441 tccttaaaat tcgtatagtg ctgttgcgct aaaaccgtcg tcggaactag aatgacaacc
     1501 tgtttgtgat cattgactgc tttaaaggct gcacgcatag caacttcagt ctttccaaaa
     1561 ccaacatccc caactaaaag tcgatccatt ggctgagaag cctgcatatc cctcttgatt
     1621 tcctcaatac tacgaagttg atcatccgtt tcaacataag ggaaagcatc atcaaaggca
     1681 tcttgatcat catcatcagc tgagaaagca aaacccttca actgactacg ttcagagtag
     1741 agtttgatta aatcatcagc tatatcctct acctggttct taaccttttg cttggccttt
     1801 ttaaaatgac cgtcatttaa tttattgagt tttggagctt taccatcact tgaaatatat
     1861 ttggacagta gatgaatctg ttccacgggg atagaaattt gatcaccatt ttggtattgg
     1921 acactgacat aatcgcgatg aattcccttg atttcaatgg tttcaattcc tagatattga
     1981 ccaatcccat ggatatgatg gacaacatag tccccttttt caagttcatt gtaatctttt
     2041 aatctctctg catttgaaac atgttgtctt cgaaaacgac gctttaattt cttttgaaaa
     2101 atctcatgtt cagttatcaa taaaatcttt tcatctacaa aatgaaaacc atgtctgaga
     2161 ttaccctcta ttaagtttac agattcttta cagatacttg tcttatctct agaatccaat
     2221 ttaatctgat attcctctaa catatcctcc aatgttttac ttcccattga attgctagac
     2281 tgcagaataa tggtgtaatc cattttttta tatcgttcaa tttcttcttt tagaaaagaa
     2341 aactgattga aaaattcctg cataggatat tgattgaatt gataaatttt gtcaaatttg
     2401 agatttccta aacccttttg aagattagag aaaaaggtca ctggactttg ttttttatag
     2461 atttgttcaa tatcagaaaa atactgcata tcagaaaatg ctttactatt ctgtaattct
     2521 tctgtaaagt actgcgctaa ttctctttca aagacttcat actgattcat caatttttga
     2581 taatcatcaa agaatattgg agtatctttt tcaatataat caaagacagt ccatgtctta
     2641 tcatagcaca aagataaaaa cttccgagag tctgcatgac tttgtttttg gtgaaaactt
     2701 gaaagaattt cttctaggta tgatttcaaa ataggtgata aagtttttga aatttgtttt
     2761 tctaaagctg actgtcctcg ttgataatcc ttttctctca aaagcatatc actagctgga
     2821 aagatagtga gttctgtctt attttctttc gataattgtg tttctacttc aaatgacctg
     2881 ataccatcaa tttcatcacc aaaaaactca attcgacaag gttctaactg ggatatttca
     2941 aaaatatcta aaatatctcc tcgaagacta aattcgccct gagtttgtac ttgagtaact
     3001 tttcgatagc cattttcctt taactgatgg ataaacgcgt gttgatcata ttcttcacca
     3061 actgagattt ttacaatact atctttgaat gcattgggag acggtaaaat caatcgactt
     3121 gctgcgatat tacaaactaa aatccctttc ttagatgaat cagtcaaaaa acgcaaggct
     3181 tcaacccgtg aaataatttt ttcctgtgaa gacatcaaaa actccaccat aggagcatca
     3241 tctaccaaaa atggatagac gagttcctca cccaagatag atataagatc actaacaagt
     3301 ccttctgctt ctccataagt tgacgtcaat aacacaatcc tatcttcttt ttctaaactg
     3361 cttgcaattg caagagcctt agtagatgtt gataaaccaa gtattagttg tcttttctta
     3421 tctgttaaat tttgatgcca ttttttaatc tgatcatttt ctgagagtaa atctaataag
     3481 gtcaccattt atccgttata cctctgcatt gttttctcaa aatttttctc ttgtaaatag
     3541 tagtttacag aatcgtcaac tttgtcaaca gactgtaaaa taccgatata atcatccctg
     3601 tcaaacttac tcaaaacatg atgaacaact gacataccat ttttaggtct tccaattcca
     3661 atcttaacac ggttaaagac ctgagttcct atatgttgaa taatagactt gataccatta
     3721 tgaccacctg ctgagccttt tgctcttaaa cgaattttcc caacttccat gtcaagatca
     3781 tcgtaaatga taagtaaatc gtcaatatcc aaaccatagt aagttaataa agcatgaact
     3841 gcttttccac tttcattcat aaaggtcgtt ggtttaacca gataaatttt ttctccattt
     3901 aggaaaaagg atgctaggtc agcttgaaat atcttatcgt gtgtaaaagt gacattctgt
     3961 ttcttcgcta gttgatcaat caacataaaa ccaacattgt gttttgtttc aaaatattta
     4021 tcccctggat ttcccaaacc tacaagtaat ttggtcattt atttttcctt tcaaaagcca
     4081 aaagggttgg aatttttttc caacctaatt gacaccattt attaattttt agacattaaa
     4141 gcggaattcc atgatatcgc catcttgaac gatatattct tttccttctt cacgcaagcg
     4201 tccagcttct tttacggcct tttcagatcc gtatttcact agatcttcat atgacatggt
     4261 tactgcacga ataaagcctt tttcaaagtc tgagtggata ataccagctg cttgaggagc
     4321 cttcatacca cgtttgaaag tccaagcgcg aacttctttt tcaccagctg tgaagtaagt
     4381 tcccaatcca agcaagtggt aagctgcacg cgtcaactta tctacacctg attctgtcaa
     4441 accaatggct tcaagaaact cttttttatc ttcatcatcc aattcagaaa tttcttcctc
     4501 agcacgcgca gaaataacga ctacttcagc attttctgtc gctgcaaatt cacgaatttg
     4561 tttgacatag tcgatagagt caggttctga aaccacatcc tcgtccacat tagctacata
     4621 aagaactggt ttagtcgtca aaaggaaaag acctttgaca accttttgtt cctcatctgt
     4681 aaattcaatg gtacgagctg atttcccgtc ttctaggact ggtttaatct tttgaagaac
     4741 attgaattct gctactgatt ctttatcttt ttgcgtacgt gccatctttt ctacacgcgc
     4801 atatcgtttg ttcactgatt ctaagtcagc aagaattaat tccagattaa ttgtatcaat
     4861 atctgcaagt ggatctacaa aggcgtcttc acgtccttgc tcgcgcatta cattttcatc
     4921 atcaaaagca cgaactacgt gaacaatcgc atctacttca cgaatattgg ccaagaattt
     4981 attccctagc ccttctcctt ttgaagctcc ttttacaatc cctgcaatat ctgtaaattc
     5041 aaatgttgtg ggaactgtct ttttaggagt tatcatttca gttagttttt gtaggcgttc
     5101 atctggaact tccaccattc caacatttgg atcaatagtc gcaaatgggt agtttgctgc
     5161 ctctgctcct gcttttgtaa ttgcattaaa tagtgttgat ttaccaacgt ttggcaaacc
     5221 aacgatacct gctgtcaaag ccatatttct cattctccgt tttcatttca atccctaata
     5281 ttataacaca aaaagggaaa acttgctaaa attccttgat atagctattt cccataactg
     5341 aaataataat tttatggact tttttaggaa ctttttaatt tttcaatagc tttttcataa
     5401 aatgaagtag catttttctg attgtctttg gataggtggc tataaatatc catggtcata
     5461 gaaagttttg catgaccgag ccgtatttgt atctctttgt aaggtagtcc agcattgagc
     5521 aagatactag catgagtgtg gcggaaggcg tggaacgtaa aacgagggca tccagcacgt
     5581 tccaaatgtt ttactaacct gaaactaagc attctagcgt ctatatgctt atcaaagcta
     5641 ttagagaata ctttctcata ggtcaacccc atttctttcc ctatttggga ttgtcttgct
     5701 ttatagagcc gtagcatgag caccgtctta ttatccaagt caattaccct aatacttgat
     5761 ttagtcttag ggccagtgat ttctttgata atatttaacg ttttatttac gtctagtgtg
     5821 ccgtttttta ggtcaatatc agaccatttg agggctaaac actcacgaat acgcaaacca
     5881 gtcgctaaaa gcgtcttata tagcaccgta tcgtagaaat ttttgtaagt gtttggcagc
     5941 tgctccaggt aagtcaagaa tttttttaag ttatcatcat ccagatattt cagtttttgc
     6001 ccttcttttt ccttacgacg tggcacgata acatcacggg cagggttaaa ggggatagct
     6061 tgcaaagata cggcatattt aagtatgcgt ttgtttagag agtgaaggag cggatattct
     6121 tggaatcctt tgcctagttg attatagtct tttgcccatt gattgacttg cttttgaatg
     6181 attgggggcg tcagtttatc cagcctataa tttccgaaag ctggaagtag atatttatct
     6241 attttgaatt tggtacccat taaggtcgat tttttaaccg tatggcagta cgtttctagc
     6301 caattctcca ccagttcggc gtaagtcttt atctccaccg ctttatatac tgtagcccct
     6361 cctttttcaa agtcgatttg agcctgtagc gctttggctt tgagttctct cttggttcgc
     6421 cctgatatag tcgtcttgac tttcttacct gttacggtat cgataccaag gtagacgcta
     6481 gagcgataga ctgtagaccc gtcttttttg gtgtattctg ttattttcat gatgataaac
     6541 ctttctaaaa tacatcagca ggcaagctat tattaaaaag gttttagatt atggtttata
     6601 tcatgttgag cttacgggaa agattttatt tgttattttc ttcagctaaa gctatctgta
     6661 caagtttttc ttttactttt tcagtgagtt cctcatagta gagaattgaa gaaataattt
     6721 cttttaaaaa attgaaatcg attaacccca ttgctaccca taagacaata ttttccattg
     6781 tttcgataaa gaagggaatg aggtaatcaa ggtgattgat tgttaaaaat ttaataccga
     6841 agacaatgct acttctctta tttccatcat tgaacatatg aaattgaact gtattaaata
     6901 ctagcactgt aagttttgtt tcaaagtctg gatagtaatc gtcattctgt aagtgttcaa
     6961 atacactaaa tacccttccc ttatctttaa caccgtgtaa accacctatt ttctcaatta
     7021 cttcaatgtc gtgtagtaaa attgcttctt cagcagaaaa ataattgatt ttatccatta
     7081 tttatctctc aatctttcaa aaacttggcg gtgttcatca atttgttccg aaagtgattt
     7141 actttgttcg ccaataaatc tttcaaaatc ttcttcagaa attggacgga tatattctgc
     7201 taatttctca tgtttggcat tcctgaaaga tagatcccta ctagccattt tctctctggc
     7261 atgcattaaa tggggttctt gcattgggtg atttgctaat ctctgaatta tctcgattac
     7321 atcagtatta gttagttttt gatttgaaga cttactcttt tgttcaatct cataagcaac
     7381 ccctttctca aaactagcaa taactataag tacctcacta tacattgttg cgcgagcatt
     7441 atcattttta ttcaatttca gtattttttt atattctgat gctctttctt taaagattgc
     7501 tttgtaaatt tcattcgtta attgaccata tttaaaatta cttccagaga catacttatc
     7561 aatggcattt gtaaatattt ttctagcatt atcttcttct aatgcagtcg atagataatt
     7621 tttgtcacgc tggtttataa attttacatg gccaccagtc ttctcagtta acacattaat
     7681 gacaatatca agtagggtat ttcttacttg cttagctata tcactattgg ttaacaacat
     7741 agcaaaatta agcaatgttc tgaacgatga gatacttaat tgtgtaactt tgctaccgac
     7801 atttgtgtcg gtagcaaaag cattttcttt aaatttagct agtttttctc cacgtaaagt
     7861 gtgataacca ttttcagtaa gctccgtttt atgagcttca acaagtcttt caatagttcg
     7921 aatgtcgact tcgaaaaagg tagctagctg agaatttgta aaataaaaag tatcctcaaa
     7981 gtagaaacca ggtactttta aaatctcttc agctttttgt aatgctaaag aattatttaa
     8041 aatattttgt ctttctattt gtgaggttgt cagatcagtc ataatttctc ctttttgtcg
     8101 agttaatttg ccaataactc ccgatatttc aagagtttta ttttattttc gtgtcgttag
     8161 agtatattag aaaatcttga actccgcgaa tacacgcgct tcaggattgt tgaacattgt
     8221 ggcgcgtggg ttagtcgctg aagtgttccc tgatttcttc agctaaatca ttcaagcggt
     8281 tctgaatacc tttatagtaa tcaaattcaa tggtgtcaat gatgtcactt ttttcagtaa
     8341 atatcttgtt ttttgtaaca caaaagctat tatttgtatt attgtgaaca aggtcattta
     8401 tctcattttg aacagaagat attttccttt gtaagcaata gcctaagatt tttcgatctt
     8461 gaatacctat tttttcaatt atttcattca cttcaaagac aactgcccca tagctatcaa
     8521 ttttattgtt aaggctattg ataagattag aaatttcaga atgcacatag gaggtattga
     8581 gagcttgctc acgttcaata acgtttatta aacgtgtaat atattcgtta gtaatcatgg
     8641 tgagtacatt attataagcg tactccctag cttcgcgctg tttggaaagt agtagagcct
     8701 cattattttt tgagatatct gataaatcaa gtccgttgaa aatttcaaca ataccagggt
     8761 ttattattga agactgaaga acgcgaccca aaacaccctg gtcaaatgct atttttcctc
     8821 gaatgtactc catattcata taatcttttt taaagctaag tatttctttg attttattga
     8881 agaacatctc aaaaaccttt tctctttgat tattatctag caactctttt agtgtgctgg
     8941 ttgaaactgt atcaataaac cgcagatcta ggaaagattt ttttacgttc aaaaaatcag
     9001 ctattttttg taaattccca gcggttggta aagattttcc ttttacatat cccgtaattg
     9061 tacttttagg aatatctaaa gcgttatgaa ggtcaatttg tctaacccct ttttgttcca
     9121 taagaaggtt gagccgttta gagaagtatt ctctattttc aagatcttga ggactatttt
     9181 tagccatgac tttaaccctt tcagttgtaa gttatacatt gattataaca caaaataaaa
     9241 acaaagtacg aaaataaata aacttttttc taaaaacact tgactaagta cgaaaataat
     9301 agtactatta acttatcact tttgaaagga attgttagat tatatggaac aatggacgtt
     9361 gaaagcctgt agagtaaacg ctggttatac tttgcgacag gttgctaaaa aggtaaataa
     9421 gaattttcaa actgtctcca agtacgaaaa agatagtaca tatataccgt ttgaactttt
     9481 aaaagaattg tctgcaattt accaagttaa attggacaat atttttttag gagatagtac
     9541 gaaaaaaata gaactaaata aaagagagtt gcaataatga gagtattatt ttaagtcaat
     9601 ggtggcagaa atcaaaccag acataacaat agatgaatta ttttatagct agaaaggagc
     9661 aaaccaatgg aactagtcta tatggacggt aaaaaagagc cgtatacact gagcagtatt
     9721 gtggcagagt gtgcagaagt caagcacaga catttgaaga ttttgctaaa taaacaccga
     9781 gaggacttcg agagtttcgg aaaggtgcaa tttaaaattt caccttcaga aagtgggcaa
     9841 aatgtacggg actatatttt aaatgaacaa caagcaacat tgctgatcac ttacttacga
     9901 aatacagaac ccgtaaaaga gtttaagaag aacctagtca aagcattctt tgaaatgcgt
     9961 gaggaacttt ctaagtttcg tatgcagagg gcgctagaaa agccaaaaag aaaaaccttg
    10021 catgacagta ttaaaacatg ggaggcaaaa cccaagcacg cgcatagcac catgaacaac
    10081 ctgttactga aggcagtaac tgacatgaac gctaagcagt tagtaaaaag ccgtgggggc
    10141 tataatggca ttgatagctt gaccagtgac gagctggagc aataccaggc atttgaggat
    10201 atggtaatag ccatgattgg cttgaatatg agttatcagg aaatcaaagc tatggcattc
    10261 agaaataaaa aaacacgcca aagaagcgtg tgagagcaac aaaaaaggct tagcaataac
    10321 cacaccgcag agcctttcac actaacacta aaacgaattt aacaaaaagc aggcaagcta
    10381 ttatcaaaga ggttttagta aagatttata cctagattat agcatattta gggcaatttg
    10441 accatacgga gagcgccaac tctttaaact ggtactttct cacgctttca atttggcgag
    10501 tctgagcgtg aggctagtac ttacaagaaa aaactgtata agaaacaact caaaaagccc
    10561 cacgctcaga agtttggcga ccgagagcat gaggctgtag gcaagaaaaa ctgtataaga
    10621 aacaaccatt caaaatcccc acactcgcca tcgctcaact ttgagtgtga ggatcagcag
    10681 gtatagtaaa aggtattaaa tggcccttag ggcaactaca caacatagaa acagaggtaa
    10741 aaacatgaca gaaacaacat acgagaattt aactagacgt ataaacagaa ttagcgcaga
    10801 aatgcaagaa attggcgaaa aaaatgggct tagtagactt tcgttattgg caagtcagac
    10861 aaaattcatc aaagaagaat tatcccgttt actttggatt gaactccctg aattgaatga
    10921 cagccataaa atcgaagcgg tctctaaaag cactacggga atgtttttcc accctggtat
    10981 ttttgaaatg gacgctatgc gacaagcatt ctttaaacgc caagctatgc acttttttga
    11041 gaacgaagca gagcaacagg cgtatataga acatgctgaa aaggagtatt tagaggctac
    11101 tataacctta aaagatattc tttttaactc taaaaatgga actcagagag taaataaaag
    11161 ttgtcttata gagaagtttg aggaggcaat gcaatgacac tagacctaga caacatgaca
    11221 caagcagaat ttgataaaca aatggctgaa atcaaggaga gaaatccaaa catttttcaa
    11281 ctgatttctg attttttaga tagaaaggta acacccgaag aggtggatga gtttttgaag
    11341 atggagcgag ctgaacaagt ggaatatatt aagaattatc aagcgagggt ataacatgaa
    11401 tgagctagat ataagtaaca cacaggctgc tgtcttaatc gtgattttga ttggcttact
    11461 gctctattta aaccaccgag accgcaaaaa aagcgcccaa attgagcgag aaaaccaaca
    11521 gacgataaaa acacctagcg aggatttaaa ccctgattat gggcgatata tccagcttgc
    11581 aggagtcaac gtatggggag ggatggaatg agttacacaa tgaatttata ccccgatatt
    11641 gaaaaaatac cagatgaaac ctattttact aaagagattt ttctagcaac gtgcttgaat
    11701 aacacaatca gaatagcagt agccagagga gtgctaacat ctcaactttt agacaaagaa
    11761 actaaagaag ttgtttgtaa aaccttggat acaatcatag aaaatcaaaa agctattttg
    11821 caaggggtag cgagcggaca aataaccttt gtaaataaaa gcaaaggaga ggaggggttg
    11881 gatgagtcta tcagagaatg acaagcgagt tttaagacta atcaaggtag gggctgagaa
    11941 ctccataaca gggtcagaaa ttagcctgac aaccaagcta acagaaagaa ctgtacgtga
    12001 cattatcaag cgtctagtag ttaagcacaa catccctatt gtaggggtta gatgtggcgt
    12061 ttttagtggg tactttattc cagcgaataa aggcgagtta ctagatggcg ctaaggcttt
    12121 ttacaaccaa gtacaggagg agagcaagcg cctagcggtg ttgatgaata gcgacctaga
    12181 aagctacaaa gaaaccttga aggaggtggg cggatatgtt tagcctaagc cgagaaagcg
    12241 agcaagacct agcgcatggc attctggagg tagtggaaag atacctggaa gcgcgtgaga
    12301 aagtaccgcc aagattgacc aagttaataa atagagttga actcaaagag gaattgaaaa
    12361 ttagtgacaa cacgctgaat aagtgggaaa gtcagggttt aagacgctat caaccgccgg
    12421 acgatggtgc tagaataata ttttatttag tggatgacat tcgtaggttt atgggggtgg
    12481 agaatgggta gatataacac acacccagcg caaggcggag gtcattatca tgacattaaa
    12541 ttgtataagc accgtggcag atctttggaa cagttccaag aacaaaagct actgaagaag
    12601 ttaaaaaaaa acgcaaaaag gggcgatagt atgacaattt atgaggctag aggtttccag
    12661 agtaatcttg tctatccatt tgataagatg gagccgttcc agtacattga acgctttaag
    12721 cctttggtag tccctgaaag tgctgatccg gaggaataca aaagaactca ggcgccatat
    12781 tgtttaagtg gtaaggttat gccggagaaa aacgggagtt acaaacgaaa caactccagt
    12841 ctaatctatc gggatttgat tttccttgat tatgacgata tacaggggac gactgaggac
    12901 ttcatagaag ccgtttctag cgctttgttt ggctactctt atatcttata cccgactatc
    12961 aaacatagca tagaaaagcc ccgttttcgc cttgtggtga aatctaacaa cgtgatgaac
    13021 gaggcaacct ataagcaggt agtaaaagag attgctgaca agattgggct accctttgac
    13081 atggccagtt taacctggtc acaactccaa gggctaccag taacaacagg cgacccagca
    13141 acttatcaaa agattgtaga gcatggactt gattatccag tccccaaggt tgaaccaaga
    13201 gcgaagcaag gaactactga gcgttataaa cctagagtaa gtggccagag gtcaatgact
    13261 atgaggatca ttgacacgct tttcaacgga tttggagacg agggagggcg taacgtggca
    13321 gtgacacgct ttgtgggatt actcttcaat aagtgggttg attgtgacct agagaccgcc
    13381 tatgaactga caaagatagc caatagtgtg acagttgaac cattgcctat tgaagagcta
    13441 gaccgcactt ttagcagtat agcacgggct gagtatagaa agagaggtta gaaccataga
    13501 actagaagaa ttagcaaacc taaaaagtga aatcatggag gtaatggagc aagaacaccc
    13561 tcctagaacg atgaaggagc ttgaaaatcg tatctttaaa gctggagaag aatggcgggc
    13621 agaacatact gaaactaagg taaatgagac cacaggggac gttaccgaaa aggttgccat
    13681 gcctcagaca ttcacagtag caaaaatact gagcgagatt gtcacattta cttttatcag
    13741 caagagcaat atacctgatt atagcctgct ctatatctat gatttagacg agggtatcta
    13801 taccgctagt aatgatctat tcaatctttt gtgtaagacc tttgacgtta gaattaaacc
    13861 cagagagtgg ccacagatta agttaatggt tagaacattg gcaaagatac gaaaaccttt
    13921 agagagtgct aaccttgtac ctgtaaagaa tggcattatt gacctaagaa ctaaggagct
    13981 actccccttt agtcctaagt atgtaattac aagtaagata agcacggcct accacgcgcc
    14041 taaacgagtc ccaacagata gagagggcaa aacttttgat gattggctaa actcaatcgc
    14101 ttgtaatgat agtgaactgg taacactgtt ttggcagatt atccttgaag ctattaaccc
    14161 taaccatacc cgaaataagt ttgctatctt ctacggggac ggtaacaatg gtaaagggac
    14221 attccagcgc ttccttatca atctgatagg ggaaagtaat atatcagcct tgaagcctgc
    14281 acagttcgga gaaaagcata acttggagac cttggtaggt aaggtttgta atattgggga
    14341 tgaagcgcct aacgaatatt taaaaaatcc gtctgattta atgagtatca ctagtggcga
    14401 cactgttcta gtcaatccaa aaggacgccc agcctttgaa gctactttca agctattcaa
    14461 tatcttttca gggaattata ttcccaatgg tggcaacaag acaaaaggct ggtatcgtag
    14521 aattatgatc gtccctttta atgctgactt taacggggag aaggaaaaac cttggattaa
    14581 aaatgagttt ttagcaaaca aggaagtcct agagtatgcc ctctacaaag ctatcaatca
    14641 agagccattt actcacttta tcgaacctaa agcagttaaa ggactcctag aagaatacca
    14701 ggaggataat gactacttac tttcatgggt taagcatgaa tacatggaaa aaggttggca
    14761 tgagctggac gtagtacctg tttttatcgt tacaagatca ctaaagcact atgccgaaga
    14821 tatgggaata cccaaaccca atgtttacgg ggctggtaaa gaaactatca agcacttaca
    14881 acagttgaca cctaatcggt attctctaag aaagaaaaga gttccttcag aagattttga
    14941 aaaactagag gcatgggacg ttgacaagat aaaaatgcgg acacctcaac actctatcac
    15001 taaacaagaa taagtgtgac gttctttttt tgcttaaacc cttgataata ctgattttct
    15061 taaaaaaagt gtgacgttct caaaaaagaa ggttacaccc cttgaagcct tgatatatct
    15121 agctttaggg gaataggtgt gacgttgtga cattctttcc aactcttata tatagagaaa
    15181 aatgcagtat ttatatatag gaacgataaa acaaaagaac gtcacagggt cacaggggtg
    15241 gtgaaacacc tagagcctca agggtttgaa cgtgtgacgt tctttgtcac agaaggtcac
    15301 agaacacgaa aaatattaca taatttacct taaaataaag aaatgacaac aacatgaaag
    15361 ttaaactatt tgaatatact agacgatggg aaaaagaaga ctttgagaaa gagatcaatg
    15421 acttcatggc aactgttgaa gtgctggacg ttaaattttc tggtacacca tctagtgaat
    15481 cctttaattc ggtggctatc gtattggtat tatacagata acaaaatggg gtagatagta
    15541 ccgaccccct tgtaatggtg taactactag tgacaccttt aaaacagaaa gaggaaaaac
    15601 aacatgacat taaaaacatt ttcggataaa gccaaaacat ttactttcac ttacgaattt
    15661 aaagacctag atactgctat ggtagcaggt cacgcgctac tgggatatat gactgggact
    15721 tatgaagtac catctatttc aataactcac aaggataaag ggacgcttgt cgctgagtat
    15781 gtggaagatc acaagctaaa caagactttc aagcgtattt gtgacagttt caaagactat
    15841 tacaaccaac cagtggacga tgaagcattt gaagaacgtt ataaacggga gcgcgtgctc
    15901 caactcaaag agtaagagga ctttgagaac ttgctggaaa aagtaacaga ctacgagctg
    15961 gaactattag actatgctga tcgcttgctt tctgataagc ctatccctat ggactctatg
    16021 accgcttttg gtacgttgga aatgctgggc gatgaaagta ttagcctact ccaaaagttg
    16081 gacgtagagg gcgaatataa aggccttgct ggttatacag agcatttgaa gtagaacaca
    16141 ggggagcaat cccctttttg ttatcaagtc aatagttttg ggtttttcca tataggggga
    16201 aataatgaag cggttagcaa tagaaacaat cactaaacca atgaagctga gaggaatatc
    16261 taagggtata gctgagttgg acggtcaacg tttagagatt gatttggata atttaatgat
    16321 tgattttggt ggagagtcat ttgaattaga cagaatagca ggaactaagg gaggtaatag
    16381 atatttcttt gccctgattg tgggagacgg tgcaggttgc tttataaacg gtatttatat
    16441 tttagttgcg gtacttgtct agatatccac aagagcaccc tgaaccgtag taaaacggat
    16501 tgccaatact actgggagct tgcactaaaa gaagccagaa aggtagaacc agggtggagt
    16561 cctagacgtg gtggatatat gtttgatagt ttccctgaaa gaccaaagta tatgaagcgt
    16621 gacaggtatc ataagcacta taagaagttt ttgaagtata ttgaaaaagg agatagattt
    16681 tggctgaatg gtttgagatt gtaggacaat atgaagtagt gtgcaattta caaaacaaac
    16741 aaatactaca tatttgacgg tttgaatgtt aaaaaagcta gatatatcaa ggatttaagt
    16801 aagtagagtg ttccaaaaga gggtgaggta agggtgaggt tacatataaa aagtacaagg
    16861 ttagtacaag gtcacatact tcaaaatagc aaggttttag caaggtacag ggcttttttc
    16921 tagtgataga accctaatat taccctaagg tatagctact tttttagaac catagaacgg
    16981 taaggttacg gtaaggtatc gaagttttcc aatggtgagg taagggtgag gtaagagcaa
    17041 aaaatgtaaa ggttttgtca aggttgcgta tttaaaagcc ccaaggttac cacaaggtag
    17101 agaggtttac cgagggttag aaaaggtgag attttgctta ggtagtggca gtaaatatgc
    17161 ctggaaaatg gcagggttat gtgtgttgaa ctccgagcga acgccgagaa agtggctata
    17221 tctccctccc cacgcacttc tatgagcttc acaccgtcat tgtacatttt ttctcacggg
    17281 gaagcatttg aagctagagc gaacatagag aaaacgaaaa aagccaagac actctctgtc
    17341 tcagctaata gttctcacaa agactattat atcatagagg aggccgaggc atgacaccag
    17401 agcaggtaaa agaaaaacta gagggcgtta agtggataaa caaagagata gaaggcttat
    17461 atttagagct tgcagcttta gaaagtggta ttatcaaaaa gcaagaacta agcaatacca
    17521 gagtacaaac aagcagggta aatacgaccg agaataaccc tataagtgtt ctaaaactaa
    17581 aagaggatac acttcagaga attgagcgac ttaatgaaga gagaatggaa atatctaggc
    17641 taatcgataa gctagccaat ccgcttgaac gttctgttct caggctgttc tacttgaatg
    17701 ttcttgacgc ttggcaagtt gctgaggaaa tagataaatc aaagactgca gtatacatta
    17761 taaggcaaaa agccatagaa cacttagcaa atgtggggta ataggtgagc acaaaaaaac
    17821 gcctagaaca atctaagcgc taaagagtag tggacggtgt gcctgtcccg tcatctcata
    17881 ctatgaagtt gcgtagcgac actatcatct ctacctcact tctctttaga ctattatacc
    17941 acaaaggagc gaactaatgg agctggataa gtttaaaacg atgatgaacg tcagagagcg
    18001 gatgacctac tttctacgtt tccagaggat ggcaggaagt gaaaaccaag ttacgataga
    18061 tgaagaggct tggaaacttg tcttacctta tcggtgggat ttgagcggtg agcatgaaaa
    18121 agcaatccgt gaggggttgg aaatattcgc ccaagacatc aacagtatag aaaacaaacg
    18181 agctagaaaa tactttatta tccattactg ttacatgaga aagaaaacaa tgagcgaatg
    18241 cgtagaaatg gcagctacta gctccactag ctaccaccga tataaacaaa tagccgtctt
    18301 aaattttgcg agaatccacc agaacggaga gctagaagtg tataagtagg cgtttgtggt
    18361 gttttgttga gaaatgttga gatttaagtg aggggggatg acatgattga agattttttg
    18421 attgatttgg agaatatcca attagaggat atggagatag attttaacat ggatatagac
    18481 atcgatttaa acatagattt tgaaatagat gttgaccttg atgatttaga tttaacctta
    18541 ctacaagaat tagaagcgta aatgttggca aatgtaggct gaggcttcat attcgtcccc
    18601 tattttgctt tggtttggac tggacaatta aaacaccatg gataaactag aagccctcag
    18661 aaacgagtct aggggctttt gtgaggtcat aatcttataa accacgcgct tcatggtata
    18721 atatacctat cagcaaccag caataaaaaa agcacgttta accgtgctag tttcttgcct
    18781 gctgaactcg tcatttattg cccttttgtg gggctttttt tgtggacttt ttgagaattt
    18841 ttaagaactt ttaatgattt ctaatcaaat gagatttttc aaaaagtcag taatatcaag
    18901 ggtttaggcg ctttaaacaa gtttaaaagc gtatagatta caatctaaca caaaaaggga
    18961 taaagtgcta accctctaac taagggttct cagtcaataa ttttattcat ttttcgctca
    19021 aaatcatatc gccccatcat gacaacatgc tcacaattac tgcattttat tttgatatct
    19081 gctcctacac gtgtaatttc ccaacaatta gcctttttac cagtcgactt gattgtacaa
    19141 gcgtgtgatt ttttcatctc aacaaaattt ccaacttgat acatactact ctccttttct
    19201 ttttcgatta tatcataaaa gaggcgagcc aggctcaacc tctttcactt aatttgtacg
    19261 aactggtgta atgagctgca tgaagtcttc gtcagtatct gctggcacaa gagtaaatgg
    19321 acgaacagct gagataaagc taatggtcac cttttcgcta tttaaagctt taagagaatc
    19381 aatcaagtaa gttgggttga aactaatggt caaatcttca ccagtaacct gatcagtatc
    19441 gatttcttcg tttactttac caacttctgg agagtgaaca tgggcgctaa caaccccatc
    19501 cttaatttca agtttcacag taccattttg agtcgcactt gataaaagac gggcacgctc
    19561 cattgactgg cgtaagttta ccacattaaa agtaatagta gtgttaaagt ctgttggaat
    19621 caagcgatct gtatcaggat agtttccttc taggagacga gtatagaagc taatattttc
    19681 gcttctaaag aggatttggt tattggcaaa gaaaatctct acagtttcga tatcatctgt
    19741 aaataccgct gaaaattcgc gtagagaacg gctaggaatt acgacatcaa aatcatcact
    19801 atttttttca agagtcaatt ttttctggct taggcgatga gagtctgttg caactgtttt
    19861 taactctttg tgttgactca atacgaagtg gacacctgtt aaaatcggac gactctcttg
    19921 tgtacttgca gcaaaggctg tttcattaat aattttcttg agtaattttg tttcaagtat
    19981 taaaggagtg cttgctgaaa tttcttggat tcgtggatat tgttcgctat cttttccttt
    20041 tagggtaatt tctgatttgc cactggttaa aacaatttga ttttgttcaa tttctttaaa
    20101 atcaagagtt acatcaggta aactagatac tacattgata aagaaagaag cttcaagaag
    20161 gatcgaacct aaagaagtaa ttaacaaacc agcatcttca tttttttgag aaataaaatt
    20221 ttcaattgaa atttgaccat ttgaaccaat taaagtaata ccttcattgg tcacgtcaat
    20281 ttttactgtt gataaaatag gaatggcatt tttagaacta atagctctct tagtagtat
//
LOCUS       DAVKGU010000027        20034 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKGU010000027 DAVKGU010000000
VERSION     DAVKGU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.027"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(43..2589)
                     /locus_tag="VW077_000966"
     CDS             complement(43..2589)
                     /locus_tag="VW077_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9455004.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLAAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(2712..3152)
                     /locus_tag="VW077_000967"
     CDS             complement(2712..3152)
                     /locus_tag="VW077_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9455005.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQIHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(3127..3996)
                     /locus_tag="VW077_000968"
     CDS             complement(3127..3996)
                     /locus_tag="VW077_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9455006.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYCFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGINDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(3974..4474)
                     /locus_tag="VW077_000969"
     CDS             complement(3974..4474)
                     /locus_tag="VW077_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9455007.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKMRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(4485..5183)
                     /locus_tag="VW077_000970"
     CDS             complement(4485..5183)
                     /locus_tag="VW077_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9455008.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     RFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(5176..5442)
                     /locus_tag="VW077_000971"
     CDS             complement(5176..5442)
                     /locus_tag="VW077_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9455009.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(5508..6434)
                     /locus_tag="VW077_000972"
     CDS             complement(5508..6434)
                     /locus_tag="VW077_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9455010.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(6509..7324)
                     /locus_tag="VW077_000973"
                     /pseudo
     CDS             complement(6509..7324)
                     /locus_tag="VW077_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7328..7648)
                     /locus_tag="VW077_000974"
     CDS             complement(7328..7648)
                     /locus_tag="VW077_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9455011.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGD"
     gene            complement(7934..9931)
                     /gene="metG"
                     /locus_tag="VW077_000975"
     CDS             complement(7934..9931)
                     /gene="metG"
                     /locus_tag="VW077_000975"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9455012.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQVYVDRMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDCLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDAVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(10055..11314)
                     /locus_tag="VW077_000976"
     CDS             complement(10055..11314)
                     /locus_tag="VW077_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9455013.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(11316..12017)
                     /locus_tag="VW077_000977"
     CDS             complement(11316..12017)
                     /locus_tag="VW077_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455014.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(12001..13200)
                     /locus_tag="VW077_000978"
     CDS             complement(12001..13200)
                     /locus_tag="VW077_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9455015.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            13486..14832
                     /gene="gor"
                     /locus_tag="VW077_000979"
     CDS             13486..14832
                     /gene="gor"
                     /locus_tag="VW077_000979"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9455016.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGVKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQVAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFTSMYSACTCNRQETRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            15029..15565
                     /locus_tag="VW077_000980"
     CDS             15029..15565
                     /locus_tag="VW077_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9455017.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            15620..15700
                     /locus_tag="VW077_000981"
                     /pseudo
     CDS             15620..15700
                     /locus_tag="VW077_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(15769..15984)
                     /locus_tag="VW077_000982"
     CDS             complement(15769..15984)
                     /locus_tag="VW077_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9455018.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            16082..16402
                     /locus_tag="VW077_000983"
     CDS             16082..16402
                     /locus_tag="VW077_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455019.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(16432..16770)
                     /locus_tag="VW077_000984"
     CDS             complement(16432..16770)
                     /locus_tag="VW077_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455020.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(16782..17501)
                     /gene="trmD"
                     /locus_tag="VW077_000985"
     CDS             complement(16782..17501)
                     /gene="trmD"
                     /locus_tag="VW077_000985"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9455021.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(17491..18009)
                     /gene="rimM"
                     /locus_tag="VW077_000986"
     CDS             complement(17491..18009)
                     /gene="rimM"
                     /locus_tag="VW077_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9455022.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(18114..18399)
                     /locus_tag="VW077_000987"
                     /pseudo
     CDS             complement(18114..18399)
                     /locus_tag="VW077_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(18400..18828)
                     /locus_tag="VW077_000988"
     CDS             complement(18400..18828)
                     /locus_tag="VW077_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9455023.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKKLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEE"
     gene            complement(19405..19644)
                     /locus_tag="VW077_000989"
     CDS             complement(19405..19644)
                     /locus_tag="VW077_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9455024.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(19664..19936)
                     /gene="rpsP"
                     /locus_tag="VW077_000990"
     CDS             complement(19664..19936)
                     /gene="rpsP"
                     /locus_tag="VW077_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9455025.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
BASE COUNT         5820 a         4450 c         3605 g         6159 t
ORIGIN      
        1 tttacatgag atagctgagt tttcttttta ttttaggctt atttatgcat ttccgtattg
       61 aagaacaact gcttcgactg cagctttttc acggctaatc aagtcaacac gcgctgcaat
      121 ttccttgatt cccataccga tgttacggct aagagcaagg tcagaaagtt gcggttcaaa
      181 gaactccttg tattccgcca agcgttgctg agtcttaaat acatgagcag gaaggataac
      241 aaagctatca aagctcatat ctcctccaag ggctgcctta atccaagccc agttttcacg
      301 cgcccaagac caagctgttt tctgagttgc ttgatgagct aggaattggt aataccaagc
      361 agacaagtcc tgtggtttga ccacaaattt gtccttccaa gaagtaatca agttttggat
      421 attatccgca tctgtactgt atgcaagagc tgctgccaac tggcgtttaa agacagcatc
      481 tgttgcgtga gtataagtat caagataaag tgctaacaag tctttagtct catgatgttt
      541 catctcatta atcagaactt gtgaacgaat agctgctggg agtcctgcaa gattctcctt
      601 gtgtgttgcg aagatttggc tagcgacttg actagcttct gcatcatttg agcgaatcat
      661 catcgaaaca gccagctgac gaaccaattc atcctcatct gattctccgt ctttagcttc
      721 aaaaccaaga cggtcatagt tatgacgagc caatttagca accagtcctt tgaaggctgt
      781 ttcagcatcc gttccttcat caataaagcg ctcaagggct gaaatcactt gagaaacagc
      841 tgaaaccacc agataagact cttccttagc aagtttatca agaactggaa gcaagtctgc
      901 ataagaaatg tgccctgcct cagccaacaa acgacgttct tgaacaattt gcagtttgct
      961 tgtgttatca agtgtctcta gctcagcaag aacagctgct aacaagtctc cttgatagtc
     1021 ggtaatatag tgggcagtat tttcagtgtt gagacgaaga gctccttcat tttcagcaag
     1081 aagagctgcg tagccaggga tttcgatact ttcagtttcg agtgtatcag gcaagccttt
     1141 ccagttgcta ttgaggggca ccacccagag acggttcttg tcttcgttct caccgatgaa
     1201 gaattgtttt tgtgaaatct tcaagacatc attttcaact ttgacagtaa gaactgggta
     1261 accaggctgt tccaaccaag aatccatgaa ggctgcgaca tcacgtcctg acgcttgacc
     1321 aagggcatcc caaagatcac taccaatggt gttgctgtat tggtgttttt caaagtaggc
     1381 atgcaaacct ttagcaaaat cagcatctcc tagccaacgg cgaagcatgt gcatgagacg
     1441 gcttcctttg gcatagacga tagcgccgtc aaagagtgta ttgatttcat ctggatgttt
     1501 aacttcgacg tggacagact gaacgccatc agtagcgtca cgttcaagag caagaggtac
     1561 tccacctgtt tggaaatctt caaagatatt ccagcttggt tcgatggtat ccacacagac
     1621 gtattccatc atattagcga aactttcatt gagccaaagg tcatcccacc atttcatagt
     1681 cacgaggttc ccaaaccatt ggtgagccaa ttcatgggcc acaacaaggg caacttgttg
     1741 acggctagca aatgtagagt tctcatcgac aaccaagtaa acttcacggt aagtcacaag
     1801 accccagttt tccatagcac cagctgagaa gtcaggaagg gcgatgtgga gagattgagg
     1861 aattgggtac ttaactccat agtaatcttc gtaaaactcg atagagcgaa cagcgatatc
     1921 cagtgagaaa tcaagatttg aaagtggatg tgctttggtt gagtagacac ctaccagggt
     1981 accattttta gttttagcgg tcaccccttg caaatcacca gcaacaaagg ccaacaagta
     2041 agaagacatg cgaggtgttg tctcaaactt ccagatacct gtttccttac ggttttcaac
     2101 atcgatttct ggcatgtttg acaaggccaa ttcaccttct gcttggtcaa agcgaagaga
     2161 gaggtcaaaa gttgctttgg cttcaggctc atccacacat gggaaagctt cgcgcgcaaa
     2221 atggctctcg aactgagtag acaagacctc cttcttgact ccatcaactg tataataaga
     2281 agggtaaatc cctgtcatgt tgtctgtaat tttaccagaa aaggcaagaa ccaattcaac
     2341 ttgaccagcc tcagccaatt cgatatgaag ggcttcattg tcatggtcaa ctgtaaatgg
     2401 acgagcttga cctgcaactt ctacagaggt gatttccaag tctttttggt ggagggagat
     2461 gcggtcactc tgtgcttgac cagtgatggt cactttccca gaaaaagtct tggtctcacg
     2521 actcaaatct aaaaataaat cataatgttc aggaacaaat tgcttaataa aatgttcaac
     2581 tgcttgcata gttttctcct attctaagtt taagagctgc tgctcttctt caaacaaact
     2641 tattatatca tattttgctt gagaaaaaag gattggacag cgatttccaa aactttcttc
     2701 cttctaatag tctacagtgg gtagaccttg cgaaattctt ctaaaacaac cttgctttca
     2761 ggtgtaaaag tcagccctac ttcacccaaa cagtctgtga aaaagtcctc atgaatctgg
     2821 cgccaatagg caagtgattt gtctccctca ccttccttaa aggcatgttg cgcagaaact
     2881 tgattgaaga gttcaacaga aacctttgta atttcgacaa tgcagacaga ctgattttga
     2941 ctatctaaaa tgacatcgaa ggtccctact tggggaagtg gttcgtcttc tagtacatag
     3001 aggtcatagg ctgatgctgt tgctgtcttt tctcctttaa acaccaaatc cgctaaaagg
     3061 tctggttcaa ctccaaaagc ccaagcatcg atttcatctc cgatcaaagg attgatttgc
     3121 ttgtatttat tccacatttc ttgcggtatc atgggtactc ctttgtaatt ttttactttc
     3181 ttcttttatg tgtttaagat gatctggatg gtcaatctct aaatcaaaaa tctctggaat
     3241 agaactgtag tggataatgc acttgatacc caactgattc attttttgta tgaaagaagt
     3301 attcagatag cctgctacag caaaattaat cttgttcttt cttgctttat cctgcatatc
     3361 tcttagcata tctaacatta ttggactttc cagatcatgc cattgactgt ttctcatagt
     3421 cgcaaaaaca aaggaagtca aatcatttat tccaactaca atctttgaaa tgcccgtttc
     3481 cagtatacta gataagtcaa aatacgctga cggtaattca atcatcgttc cgactttccc
     3541 agtaaagcca tactgacgca atactgtaat agcttgtttt aattggtcgg catcattgac
     3601 aaaaggaaag ataagagaca gattggggtt ggtttgataa acttctgtaa cgacatgtgc
     3661 ttcagcctga aattcatcca aacacgccag taaacgcctg gttcctctat agccaaacaa
     3721 gggatgccct tcgtcaaaaa actctttagt ccccactaaa caattggctt ctgtattcgt
     3781 taattcagta aaacaatacc aaacttcttc atctgagtac agggagcaga tagtctctag
     3841 ataatctttt acaaattgct gacaacttgc taatatgatg ttttgattaa gctctctcaa
     3901 caaatattcc ccacgaatca tgccgacgtg gtgaaatagt tgaggataaa ttttttcaag
     3961 aattttttcg ccactaaggg caagttgatt tctcatcatt caccttccaa ttcatgtaag
     4021 aagtcttgtc cagttctgga aatcctaata attcagactt aaccttcaag actaatggcg
     4081 atgcattttc ttctgtaatc tcttgaatat ccatccaaat atatccaagt gaatcattcg
     4141 caccatcaga cacagcttcc gaagtcgtaa cttgaggtgc actctcattc atttcaacat
     4201 catacaaggc catgacatgg tgaaccataa aatttgttaa ctcttctctg acgaaaacat
     4261 cgtagattcg aggattagag tagcttctaa cagtaaatcc cgtctcttcc ataacttctc
     4321 tagtcagcgt ttccgtcagt ccttcaccaa gttgctgact gcctccaggt agatcatacc
     4381 gatgttgata agggcctctc attttttcaa tgcagagtaa cttcccattt tctaagcaaa
     4441 cagcgtagac tccaaagtgt tttttgattt ccatccaact cctcctactt caaagaccag
     4501 ccaccatcta ttgtcaagat ttgtccttgc atggcgctcg cttttccact tgctaaaaaa
     4561 agactaagct ctgctatttc ctctggctca atccagcgct tgattggggt ttcactagcc
     4621 acccagtcag ccaaaccacc tggttcaaaa tccgcagcgg tcatagctgt cttgactgct
     4681 cctggagcga taccaaagac ctgaatccca gcttcagcat agtctagagc caactgcttg
     4741 gtgaagccag ccaaggcatg cttggatgaa gtataggcgt gaccacctcc acctgctagg
     4801 ctagaagcaa tggaacacat attgatgatg attccctttt tattttccag catttgtgtc
     4861 aaataatacc gagtcaactc tactggaata atgtagttga tttcaaaaat ctcttgaatg
     4921 tcctgcgccg tttgttccaa cagtggtttg taatcatcca aaactccagc agtattacac
     4981 aaaacatcca cctgagggca ccagtcaaaa ataggttcca agtccaaggt caaatctctc
     5041 tgtaaaaagc gaaaatcacc ctctaagagt ggcttttcac cttggtcaac tccataaact
     5101 tgatagccct tctctaaaaa gaggcgagct tgagccaatc cgatccctga actcactcct
     5161 gtaatgagta cacgtttagt catgcacttc tacccaatcc gttgccaaaa catcacaaac
     5221 tgtcgggctc cacatggaaa aaccttctcc ttcgccagaa acgttgatta ggaaataagg
     5281 tgtcatttca agtgcaagcc cattttgctc gatggtatca aagagttgga catagttttc
     5341 cgcacctccc caaccagttc gtacatattt tctcttagcc tttaacccag gcaggatctc
     5401 ttcaaatgtc atgtttttct cctttaattc tacattcttc atttaattat agcaaaaaac
     5461 cgctttatac ggctttttga atgtgagtta ttcaaacctg ctactactta cggcaaatta
     5521 ttccctgcag caagataaat ttcataccat tcttttcttg ttaagctaaa gtttgccgct
     5581 cggctaactt ctctcaagtg cttaggattt gttgtaccta cgactgcctg catttttgct
     5641 ggataacgca atatccaaga aatggcaata gttgaagagg ttactccata tttaatagct
     5701 aaacgatcaa gtacttgatt taaagcttga aatttctcat ttccaacaaa attcccttta
     5761 aaatacccga attgtaagac agaccatgct tgaatgacca catcgtgtaa ttggcaatat
     5821 tcaaaaatgc tgccatctcg catagctgct tgactatctt ccatattaac atgaaaagct
     5881 gattcaaatc ctggagtaaa agccgcactc aattgtagct gattaacagc taacggctgc
     5941 ttgacatctt ttttaagcaa ctccatcatc ataggatttt gattagaaac tccaaaatct
     6001 cgaactttac cttgtttata aaggagatta aaggcttctg ctacttggtc agattccatc
     6061 aaagcatctg gtcgatgaag gagcaagcta tctagatgat caatcttcaa tctttgcaaa
     6121 ataccgtcta ctgattttat aatatagtcc ttagaaaaat caaaataggt aaattcttca
     6181 atgcgaatgc cacatttgga ctgaatccac atcttttctc ttaaatctgg acgatttttt
     6241 aggacaagac ctaacagttc ttcacaacga ccacgaccat aaatatcagc caagtcgaag
     6301 gcattgattc caacagaaag tgctgtttct acaagctctt caacttcttt tacagattta
     6361 tcttttattc tcatcattcc gagaacaatt tctgataatt ctttgtcatc ttgaccaaga
     6421 gttatgtatc tcatcaaatt tttctccttt aatttctaac attcttccct tcattataac
     6481 aaaaaaccgc tttgcaacga ctttttgact atacttcact ccattttatc ttcttaaacc
     6541 cacggaacaa gacaaagatt ccaataaaga ggacagctaa aggaataact tttgtaagga
     6601 aaacatttga aattcccatc cactcataat aacggagcag agaacccacc acaagatggg
     6661 caataatcat actgacaaat ggacgaaaga ccgcttcttt ccaattccaa ataccgataa
     6721 ctagcgaaat cgtaaagata gacaaactat cccagggagc cggaatataa aaggctcctt
     6781 cttgtatgaa gcttgccatt cctacatatc ctaaaacaac tagaagaact atagtcccaa
     6841 caacaatgta agtgccaatt ttcattttag gagaatcttg gactaaactt cttcgtaaaa
     6901 ttgtggccac aagtccaaat ccaatcagaa aaataagaag ttaccctaaa aatgtgagca
     6961 aattgactgt taagagagga cctttagaaa aatcacttag tagttgataa taacgtaata
     7021 ccgccaggac aagaattggc gtcaaaaggg actctttgat agaactgcga ggtgctccct
     7081 tgagaatctc tttcattatt tttttaggat tcttacctag ataatcctct gcactcatgc
     7141 catctcgttc tgcttctgag aaatctagca tcatcaaata gatctgctct ctgagatagt
     7201 cttcatcata gagaaatcca gcaagattaa aactttccca caactcctca aaatactttt
     7261 gattctcctc agaaaactca tgtagcaaag cgcttgtttc ttcgtaatac ttcattttct
     7321 tcatggttta atccccattc ttaatccctt ctactttttg actcaaatcg tcccattgtt
     7381 gccaaaagac tgagacacgc tcttctcctt ctttcattaa tgaaaaatac ttccgatctg
     7441 gaccatctgg cgacgggcgc atgtcgcctc ttatccattg atttttttct aacttttgca
     7501 acaaaggata aatagttcct ggaacgatag tatcaaatcc agcctctcgc aaagtctgaa
     7561 ccaactcata accataccgc tctttttgac caatcatatc caagacacaa ccttcaagaa
     7621 caccttttaa tagctgagtt tctttcatca cttctccctt ctaatctatt ttgtaatacc
     7681 tactagtaac ttcacctata gtatattact tctacactag tttgtaaagc ataatagtta
     7741 atactcttcg aaaatctctt caaaccacgt cagcgtcgcc ctaccgtatg tatggttact
     7801 gacttcgtca gttctatcta caacctcaaa gcagtgcttt gatcaacccg cggctagctt
     7861 cctagtttgc tctttgattt tcattgagta taaataaaaa aacagaacta gtctgaacta
     7921 gtcctgtcta cttttaccca atcacacttc catttggtac agctggatca actgtgagaa
     7981 gggttaattt gccatcatgt tcagctgaga gaatcatacc ctggctgaca tattttttca
     8041 tcattttacg tggtttgagg ttagcaacga tttgaacttt cttgccgacc aattcttgtt
     8101 catttggata gtattttgca attcctgaaa gaatctgacg atcttctcca tcaccagcat
     8161 ccaagcggaa ttgaagcaac ttatctgaac cttctacttt agacacttct ttgacttctg
     8221 cgacacggat ttcaaccttg tcaaagtctt caaacttgat ttcatccttg tttagtttga
     8281 gctcaactgc gtccggattc cattcttttt cgactgctgg tttattgcct tccatttgtt
     8341 ccttgatata ggcgatttct tcttccatat ttagacgtgg aaagataggt gttcctttgg
     8401 caactacagt cacatctgct gggaagtcag ccaaactcaa gttttcaaga ctagaaactt
     8461 cttccaaacc aagttgagtc aaaactgcac gactagtttc catcataaat ggttcaatca
     8521 agtgagcaac tacacgaatg ctggctgcca agtggctcat gacacttgcc aattggtcac
     8581 gaagagcttc atccttggcc aagacccatg gtgcggtctc atcgatgtat ttattggtac
     8641 gagagatcag agtccagact gcttcaagcg cacgtggata gtcaactgct tccatgtgtg
     8701 tatggaagtc tgcgattgat tgttctgcaa cctcagcaag aacatgatca aattcagtca
     8761 caccttctac ataggcaggg atttgtccat caaagtactt attaatcatg gaaaccgtac
     8821 ggttaaggag gttcccaagg tcattagcca attcatagtt gatacggccg acatagtctt
     8881 caggagtaaa ggttccgtct gaaccaactg gaaggttacg catgaggtag taacgaagtg
     8941 gatctagtcc ataacgctct accaacattt cagggtaaac gacattccct tttgacttag
     9001 acatttttcc gtctttcatg acaaaccaac catgggcaat caaacgatca ggtaatttaa
     9061 catccaacat cataagaagg attggccagt agatagagtg gaagcgaagg atgtcttttc
     9121 ctaccatatg gaagactgtt ccattccaga acttgtcaaa gttaccatgt tcgtcttgag
     9181 cgtagccaag agctgtcgca tagttaagaa gggcatcaat ccaaacgtag acaacgtgtt
     9241 ttggatttga tgggacaggc actccccatg taaaggttgt acgagatacc gccaagtctt
     9301 ccaagcctgg ctcgatgaag ttgcgtagca tttcattaag gcgaccatct ggcgtgataa
     9361 attcaggatg agctttgaaa aattcgacca aacagtcttg gtatttgcta aggcgaagga
     9421 agtatgattc ttcagaaacc cattcaacct catgacctga tggagcaata ccaccagtca
     9481 catttccagc ttcatcacgg aaaacttctg ccagctggct ttctgtaaag aattcttcgt
     9541 ctgatactga ataccaacca gagtattcac ccaagtagat atcatcttga gcaagtaagc
     9601 gttcaaagac ctgtgcgaca actttttcat ggtagtcatc agttgtacgg ataaatttat
     9661 cgtatgagat atctagtaat tgccagagtt ctttaactcc aaccgccatt ctatcaacat
     9721 agacttgagg tgtaatacca gcttcttccg ctttctgctg gattttctga ccatgttcat
     9781 caagacctgt cagataaaat acatcgtagc ccatcaggcg tttgtaacgt gctaggacat
     9841 cacatgcgat agttgtgtag gcagaaccga tatgaagttt cccagatgga tagtaaatcg
     9901 gcgttgtaat ataaaaattc ttttcagaca taatttttcc tttccaggca aatgaaacct
     9961 gtttttctaa cacttcatta tatcacattt ttaatgaatt tcgataggga aatccataca
    10021 aaaacaagat agacgagtgt ccatcttgtt gatcttattc ataacgaagg gcttcaattg
    10081 gatcaagttt cgatgccttg ttggctggca agactccaaa aatcatacca acactagccg
    10141 aaactgcaag actaaatagg gcaactggga ttgatactcc aacttctata ccttctatta
    10201 aaccttgcag taacaaacct gctaaggcag ttaaaccact tgcaattgtc aagccaatta
    10261 agccacctaa caaggtcaaa atcatggatt caatcaaaaa ctgaattaaa atattggcac
    10321 gtgttgcacc caaagcctta cgaagaccaa tctcacgagt gcgctctgtc accgaaacca
    10381 gcatgatgtt catgacacca gttcctccaa caaagagaga aatccctgcg atggaactaa
    10441 taatcgtcgt cataaaacta aacgattgtt gaatttctgc aaatacaacg gactcatctg
    10501 ccacctggta ttctccctgt tgtaagcctg caagctctgt catttttcgt gccagttctg
    10561 gacccagagt tggggttaaa ctggtatcat tcactcgaaa gacaatacta gctatttcat
    10621 ctatattaaa attcgcagca agggagatat tggtagtaat aggcaagcca ccaaacccat
    10681 atatttttga ccttttagcc tccggactag tataaacccc aatgacccgg taactaaatc
    10741 cattgacttc tacaaccttg ttaatagcct cttgaggaga ttcaaataaa ctaatggaca
    10801 attcctcatc tagcaaaata acacttgcaa actctttgaa atcttgctct ctcagactac
    10861 gacctgcaat aatttcattc ttaacagcat ccatgtaagt tctgtttcca cctgtcaaat
    10921 tagcattctc aaccttttta tcttgatagg tcaagatggc attcgttgaa ttggttacat
    10981 agtaactatc cactcccttc agtttagctg cctcttgaac ccaggattct tgcggttttg
    11041 gcggttcaac aggaacttcc tcttcctttc cagaaaccgt aaaagctgat tgtttctgag
    11101 taaaagaccc atctttactt tttttaggag agaaaaagac gctaatattt ttctgagatt
    11161 tagtcatatc tttattgact tgacgagata gggaatcacc caaagccata atcacaacaa
    11221 ctgatgaaac accgataata atcccaatca tagtaagcaa agaacgcatc ttgtgagcca
    11281 tgatagatga aaaggcaaat ttcagattct gcatcttagt tttcctcctt tcctaactga
    11341 gcactgtcag acgaaatgac cccatcccga atgacaatct gacgtttggc ataggcagca
    11401 atctcaggct catgcgttac catgataatg gtttttcctt ctttattcaa atcaaccaat
    11461 aattgcataa tttggttacc tgttttggta tccaaggctc ctgtcggttc atccgctagg
    11521 ataatagaag gattgtttac caaggcacgc gcaatggcta cacgttgctt ttgaccacca
    11581 gataattctg aaggtaaatg gtgactacgt tctgtcaatt caaccttgtc taaatattcc
    11641 tcagccaact tgcgacgttt tgaagacgaa actcctgcgt aaatcaaggg caattctaca
    11701 ttttgcagag cattgagctt cgatagaaga aagaactgct gaaagacaaa accgatttgt
    11761 tggttacgga ccttagccag ttgtttttca ccaagcccag ccacttcttg accttcaaga
    11821 taatattctc cactggttgg tgtatccaac atgccaatcg tattcatcag agtggactta
    11881 ccagacccag atggtcccat gatggctaca aattcaccct cattcacttc tagattgata
    11941 tttttgagaa cctgcagttc ttggtcacca ttacggtagc ttctgaagat attttttaga
    12001 ctaattagtt gcttcatcag ccttcacctc ttttccttct tccaaggaag atgttggatt
    12061 actgatgacc ttagcaccat ttgtcaaacc agaagtgatt tcttgatttt ctgcgtcagc
    12121 atttcccaat gaaacctcaa cttttttagc cttttgttgt tcatccacaa tccagacata
    12181 atttttacta tcatccatta ctagactgct aacaggaaca agaatagcct tagttttgct
    12241 tttaacctca atgttgacag aaaaaccttg tttcaaatca ccaacctcgc ctgtcacatc
    12301 aatagtataa gggtatttag aacctgtatt attcccggct gctggactag ctgcttcacc
    12361 attgttttta ggatagtcag aaatatagct taatttccca gtccattttt tatcaggata
    12421 cactttagaa gtaaagctta cttcttgacc tacagaaagg ttggctagat tgtactcaga
    12481 caattctccc ttgacttgta aattttcatt gctgacaata tgaaccataa cttgactcgc
    12541 ccctgttgga gatttagaaa cattgctatt gacttcgacc acagttccct ctagggtact
    12601 gagaacagtt gttgcatcca attgactttg agccttgctt aattgcgctg cagcatctgc
    12661 acgcgcatca cgggcatcac ccaattgagc atcaatagaa gcaacagaat ttccagccac
    12721 tggagttggg ctttgcaccg ttgcatcttc tcctcctact ggcgctggta actgtggagc
    12781 ctgagctgaa gcggcttcat ttcgtgcttg attgagttca ttgatatgac gatctgcctt
    12841 agctactgct cgactagctg aatcataggc cgcctgcgct tctgaactac tgtacttgac
    12901 taaagcctgc ccttcgctga ccttatcgcc cacagaaaca aggatttcat ctaaatcacc
    12961 cttactagca tcaaaataaa catattgttc attttttgct gttactgtcc ctgacaataa
    13021 aacagaggat gccacgcttc cttccttggc aacaacaaga tgagtaggct catcttttac
    13081 agcagtctga gaaggttgtc taaagagtaa aatccccccg gcacccaata caactacact
    13141 cgcagcaccg attgctgcat acagttgcca ctttttagct ttaccattct ttttcttcat
    13201 aatgaaactc cttttctttt ttacaatact ttgctattat accaaatttc cctccagcaa
    13261 acaatgcagt tcagatggaa ataatggcag ttcaggatta aacaatcgtt cggaattttg
    13321 cttttctatt gtactagata tataatacac tattttgact tatttggcaa gcttttttgc
    13381 aattttttgt gcgtagcaga tttttgcaat caaatcaaaa aaagaataaa atataactat
    13441 caaaaaagac gctctactat ttaaaagatt acaaaggagt tttcaatgag agaatatgat
    13501 atcattgcta tcggtggagg tagcggagga atcgctacca tgaaccgtgc tggtgaacat
    13561 ggagtcaaag cagccgttat tgaggaaaag aaattaggtg gaacctgtgt caacgtcggt
    13621 tgtgttccta aaaaaatcat gtggtacggg gcgcaagtcg ctgagacttt ccatcaattt
    13681 ggagaagact acggctttaa gactactgat cttaactttg actttgcaac cctacgtcgc
    13741 aatcgtgaag cctacattga tcgcgctcgt tcttcttatg atggtagttt taaacgcaac
    13801 ggtgtagact tgattgaagg tcatgctgaa tttgtagatt ctcatactgt aagcgtaaat
    13861 ggtgaactga ttcgtgctaa acatatcgtg attgctacag gtgcccatcc aagtattcct
    13921 aatattcctg gtgctgagct aggtggctct tctgatgatg tatttgcctg ggaagaactt
    13981 ccagagtcag ttgccattct aggcgctggt tatatcgccg ttgaattagc tggcgtactc
    14041 cacacttttg gtgtcaagac agatctcttt gttcgccgcg atcgtccttt acgtggtttt
    14101 gattcctaca tcgttgaagg tttggtcaag gaaatggaaa gaacaaactt accacttcac
    14161 actcacaaag tccctgtcaa gttagaaaaa actactgacg gcattaccat tcatttcgaa
    14221 gatggtacta gtcacacagc tagccaagtt atctgggcta caggtcgccg tccaaacgtt
    14281 aagggcttgc aacttgaaaa agctggagtg actctgaacg aacgtggctt tatacaagtg
    14341 gatgaatacc aaaataccgt cgttgaggga atctatgctc taggtgatgt aacaggcgag
    14401 aaagaactga ctccagttgc aatcaaggct gggcgtaccc tatcagaacg tctctttaac
    14461 ggaaaaacaa ctgcaaaaat ggactactca actattccaa ctgttgtctt ttcacaccct
    14521 gctatcggaa ctgttggttt gacagaagaa caagctatta aagaatacgg tcaagaccaa
    14581 atcaaggttt acaaatcaag ctttacttct atgtactctg cttgcacttg caaccgtcaa
    14641 gaaactcgtt tcaaattgat cacagctggt tcagaagaaa aagttgtcgg acttcacgga
    14701 attggctacg gcgttgatga aatgattcaa ggatttgctg ttgctatcaa aatgggagca
    14761 accaaggctg actttgacgc aactgtagcg attcacccaa cttcatctga agaatttgta
    14821 accatgcgtt aatcgaaaca aaagaagctg atataaatat gtctgcttct tttttaatga
    14881 ttttcgaatc aacaaaaaaa ttatccaact tgactagtca cgaaaatcgg ctgttacagt
    14941 ttttgttacc ttgtttttaa aaaatggttg acatcgactc ctatatgagt ataatagtta
    15001 atatacatta gaaaagaggt taactatttt gaaaaaagct cacgtttatg ctatccctgc
    15061 tattggggct gctctcattg ctgttttggc acaaatcagt cttccaattg gacctgttcc
    15121 cttcactctg caaaactttg caatcggctt gattgctact gtctttagac cgagagaggc
    15181 tgtactttct gctggactct atcttcttct aggtgctatc ggtcttcctg tctttgcagg
    15241 aggtggagct ggttttcagg ctttagttgg ccctactgca ggctatcttt ggttttatct
    15301 cgtttactct ggacttactt cctctctaac caacagcaag agtggtgttg ttaagatttt
    15361 tcttgcaaac ctcttgggtg atgcccttgt ctttgtcggc gggattctca gcttgcattt
    15421 cctagctgga atggcatttg aaaaagctct tgttgtgggg gttcttccct ttatcattcc
    15481 agaccttggc aaacttctgg ctattagttt tattagccgt cccctacttc aacgccttaa
    15541 aaatcaggct tactttacta actaaaaaag gataccgagt tatcatgact caatatcctt
    15601 tttttatttt gaaaacttat actcaatgaa aatcaaagag caaactagga agctagccgc
    15661 aggctgctca aaacactgtt ttgaggttgt ggatgaaact gacgaagtca gtaaccatac
    15721 atacggcaag gcaaagctga tgcggtttga agagattttc gaagagtatt agttgccttt
    15781 aaaggcatcc accatcgttt gaaattcttc atttgagaga gtaatccctt tgcccatttt
    15841 agtatggtct ggactccaag cacgaatatc aaactttgca ggggcaccat taaagctcac
    15901 acggttaatt tccttggtcc aacctttttc gttttcagaa agagtcaaca agtgctcttc
    15961 gatttcaaat gtaaattctg ccattttctt ctcctttttt agtttcatta gtttattcgt
    16021 aaaatcttgt agattttagg aaaattttat ataatattga tataaaagaa gggaggccaa
    16081 tatgagacat aaattccagc aagttctaaa taaaatacat gattttttaa atggatatga
    16141 ccaacctgac cagactgaaa ccaactccct tacagccact attgaagagg ctatccagaa
    16201 acaaaccgct gttcacctta tcttatctga gacaagcttt acaggtgaca tcatcaaata
    16261 tgatcagcaa ggccagcaaa ttatcgtgaa aaatttttcc aaaaatgtga gccggattat
    16321 ccgtataagc gatattcaac gcctgcgatt tgtcccctca actgtccaaa cagcccaaaa
    16381 aaatagattt aagaaagagt gagatgtagt tgcttcatcc cactcttttt cttagcgaat
    16441 ttgttcaaaa tgtaaatgaa ctgcgatatg atctccataa ccacttcttt ccaagtcacg
    16501 ttgtaaacga taggaaatat agtgttctgc aatggtaatg taacctgcgc ccaataaacg
    16561 atgttcaacc atagattgaa tcatactgat agtcgcacgt tccaccttgg cttcttgtaa
    16621 atccaaaact accttcttag tgacttgagc aagattttga cgcaaatcat ctgtcaaaac
    16681 ataaacagtt tgggctgcct tcaagatggc ttggtaaatc ttatctggat taaattcagc
    16741 aatttcgcca ttacgtttga ttacttgcat aggtttctcc tttattcttt gttttctttg
    16801 atttctgcca gcattttttc ttcttctact gtcagttgat aatgttcaag taaatctggt
    16861 ctgcgctcgt aggttttctt taaactctcg tacaatcgcc actgacgaat cttttcatgg
    16921 tggccactca tcaatacatc tggcacgacc atgcctcgat aatcataggg acgtgtgtac
    16981 tgaggatatt ctaaaagacc tgaagaaaaa ctatcatctt ggtggctaga ctccttgcca
    17041 atcacttctg gaatcaggcg aactgtagca tcaatcatgg tcatagctgc caattctcca
    17101 ccagtgagga catagtcgcc tagggaaatc tcatctgtta ccaaggtctt aatgcgctca
    17161 tcataaccct catagtgccc acagataaag attagctctt cctcttgagc caaatcttca
    17221 gcataagcct gatcaaactg ctttccagca ggatcgagga gaataacgcg cggatttttc
    17281 ttttcaatag catcaaagga atcgaaaata ggttgtactc tgagcaacat gccctgaccg
    17341 cctccgtagg gctcatcatc tacatgacgg gccttttcag cattttctcg aaaattatga
    17401 tactggatat ccaagagccc tttttctcga gcctttccaa cgattgagtg ctccagtgga
    17461 gaaaacatct ctggaaagag ggttaaaata tcaatcttca tcgtctaacc cttctaagat
    17521 ttccacatcg acccgtttat ttggaatatc aacattgaga accactggtg ggatataagg
    17581 taaaagcaaa tcacgtttgc cttttcgttt gaccacccag acatcattag cacctggttg
    17641 caggatttcc ttgatggttc caaccaagct atcaccctca tagacttcca aaccgataat
    17701 ctcgtgatag taaaattcac catcgtctag gtcattcaaa tcttcctcag cgaccttgag
    17761 actgtatccc ttgtactttt cgatagtatt gatatggtac atatctttga atttaataat
    17821 gtcaaagttc ttctgtttac ggtggctagc gatggtcact gtttggacaa actgatcttt
    17881 ttcatcaaac aaagccagct cagctccttt tttaaaccgt tcttctgcaa aatccgtcac
    17941 agacaagact cgcatctcac cctgtaatcc ctgcgtatta acgattttcc caacattaaa
    18001 gtagttcatc ttgtctcctg taatctcctt ttttccatct tattctgaca attctcgaat
    18061 aatagccgca attttttccg attctgacca ttgtaaataa tggtgattcc ttcctaaaat
    18121 gagtttagta ttggaagtcc aatattctga ttctctgtac tctttttctc tataagtctg
    18181 acaaaaaata aatacaggga catgagattc tatcgacaaa ttctcaaaat cttcctcagt
    18241 aatctctcca gatatctgaa attctggatc ttgattttcc aactctaagc ctttttcttg
    18301 cattaattcc cagatttttt tattcgtttc aggactaaat gttgcttgag ttaagttctt
    18361 aaaataaagt tcaggaccac actcgtcaat cagcctcatc tactcttcca tttctggata
    18421 aggattttct gaaaaatcag caaacatgac ttttttagtt gtcggttcaa ttgctactaa
    18481 agtctgacgc ttaattggtt tctcgagtaa tttgcaagct aaaattccac tccaactatg
    18541 tgcacaaagt atatattcag aaattcctaa ttcttcaagt acttcataaa ccgcatctgc
    18601 aagattatct agtttttttc cagcttggtc atgaatcgga ctcctacctg tgttcggaaa
    18661 atcaattgtc aaataaccaa ttgtaggagg aggtttttca agtataagtg aaaaattttc
    18721 ataacttggt agcaaacctg ctccgtttag acaaactagc actttctttt gcttttgata
    18781 agtaacagag aggctaccaa tttctgtaga tacttcaaac ctcttcataa agaaatccac
    18841 tgattctata taatgaatta ttaaaaatcc ttatccttta ttttatcacg ttccaaggat
    18901 tttctcaagt tggaggaagg ggacaacatc tctactttcc cttcaagaat ccttccaaat
    18961 tatgtttatg ttggtaatta acggctgcgg ttttgtcttt ctcaaagaca gtcttggtaa
    19021 ggtcaatatg attaatagct acgattgcga cggtgtagta aatgatatca gccagttctc
    19081 tggcaagttc ctcgttcgaa tcctatccct tcttttcgac cagagcgcct attcaaaacc
    19141 tcggctactt ctccgacttc ctccactaac ttcataaaga gaccttcatc agtccgagac
    19201 tgctgttaat gttcgattaa gtagtcttgg aattgcctaa acgttcaatc ttttatagta
    19261 tattgaaact agaatagtac acctttactt ctaaaacatt gttagaaatc gatttgactg
    19321 tcctgatcga tttgtcctgt tcttgtttca ttttactata tcttctattc cacacaaaaa
    19381 agcgagacat ccgtcccgcc cttcttattt ttcgtcaata acgattctta cttttttgta
    19441 ttcagttggg acagagtaga caatcgttct tatcgcagaa atagtgcgac ccttacgacc
    19501 gattacacga cccacatcgc tttgatcaag attcaaatga tattccaaaa attctggtgt
    19561 atcctcaatc ttgatagtta aggcatctgg ttgtgaaatt aagggtttca caatcgcaat
    19621 aatgagattt tcaatcgtat ccatctgtca acctacttta aacttatttt gaaaatttag
    19681 aatcgtggaa ttttttcaat acgccttctt ttgaaaggat gttacgtact gtgtctgaag
    19741 gttgagctcc attagccaac catgcaagaa cgcggtcttc tttcaaagtt acttggtttt
    19801 cagcaacaag tgggttgtaa gttccaactg tttcgatgaa acgtccgtca cgtggtgaac
    19861 gtgaatctgc tacgttgata cggtagaaag gttttttctt agaacccata cgagtcaaac
    19921 ggattttaac tgccattttt aaagtctcat ttctttaatt ttttatttcg gtgaaatagc
    19981 tgagctattt agcacatgtt ctattatagc agatttctgg caggtgtcaa gaaa
//
LOCUS       DAVKGU010000028        19518 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKGU010000028 DAVKGU010000000
VERSION     DAVKGU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.028"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            48..401
                     /locus_tag="VW077_000991"
     CDS             48..401
                     /locus_tag="VW077_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9455026.1"
                     /translation="MFSTLEKIKELAKKRGIPLTKLEESLGYSTNYFYTLKTKTPNSE
                     RLQQIADYFNVSTDYLLGRTDNPAIAGDDTISGYTSDDLRKMAENAKTFDGKPLTEED
                     IDAIQNIIEIYLRGR"
     gene            403..1497
                     /locus_tag="VW077_000992"
     CDS             403..1497
                     /locus_tag="VW077_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455027.1"
                     /translation="MTITEQDALLFNTLFENIYHQLPSVDSSKDYWFVRAQKGQFYKS
                     FQTGGYIAIGWNYITLDDLKNLDEDAIKGKIKEFDSRIEKPGSAYNQMMKFAYSLKIG
                     DIVIVPSESPNDFLVGEITSRPYTESESNIESASNVCPFNKRMNVHWFGVIPNRDIDP
                     KLYKLVYSGHTITDANAYKKFINRGLYDAYIDHDHMSITFKVQEENNIDAFEYSTFLH
                     TVLQMVNVVKESDELKDEKVVLRTNVQSKGPIELLGHPEILIPVLVFIIILTGGIAFR
                     NLIKRNGANFEVDSKLGKFKAQLNSDGDEAIKKAQANKINAEAIALLIDKGMSPQFKG
                     ATSQLDIKAPEVATRILQDELLESPEKVEE"
     gene            1720..2100
                     /locus_tag="VW077_000993"
     CDS             1720..2100
                     /locus_tag="VW077_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU9455028.1"
                     /translation="MTIEELVDSHGVTLAYFDNELWQRPGVYIKEISIIFINRELSED
                     AKKRVIYHELGHLEHSTALYQNNHTRCENEANRHMIHKLLEEELALSDDHQSFNYIQF
                     MQKHKLRTVTDELMVIDEYYELIG"
     gene            2242..3369
                     /locus_tag="VW077_000994"
     CDS             2242..3369
                     /locus_tag="VW077_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9455029.1"
                     /translation="MWMEELPNGKYKFFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKINKKLSTKQVESITFEEIYNLFYKSWAQTVKESTKHNCKSVDKKMKEVIPSD
                     TILANLDRRFLQEAIEKIIESNGYITAKKVRHRLRGIFNYAVQYSYIENNEVDYTTIP
                     QKPKTLEELEKKRNNFLTMQEIKALVDVLNRREYHQKYADMVLVLTLTGMRYGELTAL
                     QLKNIDFENNKIEITGNFDSVNKIKTLPKTTNSIRTIKVSESVIEAIQRQIVRLSERF
                     QPLSSDDYIFCFEKWNQPTTIACFIQILKKYGKQAKIEKNLSSHIFRHSHISFLAESG
                     LPIKSIMDRVGHSNAKMTLEIYSHTTEDMEDKLVNKLDTIF"
     gene            complement(3457..4368)
                     /gene="whiA"
                     /locus_tag="VW077_000995"
     CDS             complement(3457..4368)
                     /gene="whiA"
                     /locus_tag="VW077_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9455030.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(4365..5342)
                     /locus_tag="VW077_000996"
     CDS             complement(4365..5342)
                     /locus_tag="VW077_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9455031.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(5339..6229)
                     /gene="rapZ"
                     /locus_tag="VW077_000997"
     CDS             complement(5339..6229)
                     /gene="rapZ"
                     /locus_tag="VW077_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9455032.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(6281..6661)
                     /locus_tag="VW077_000998"
     CDS             complement(6281..6661)
                     /locus_tag="VW077_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9455033.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(6672..7259)
                     /locus_tag="VW077_000999"
     CDS             complement(6672..7259)
                     /locus_tag="VW077_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9455034.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(7268..8500)
                     /gene="clpX"
                     /locus_tag="VW077_001000"
     CDS             complement(7268..8500)
                     /gene="clpX"
                     /locus_tag="VW077_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9455035.1"
                     /translation="MPTTRNNDMMVYCSFCGKSQEEVQKIIAGNNAFICNECVELAQE
                     IIREELAEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(8537..8707)
                     /locus_tag="VW077_001001"
     CDS             complement(8537..8707)
                     /locus_tag="VW077_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455036.1"
                     /translation="MERSIFGFFTAMLCLVCLLAGAQAFRKKRYGLSVLLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(8707..9213)
                     /locus_tag="VW077_001002"
     CDS             complement(8707..9213)
                     /locus_tag="VW077_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9455037.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWYLPAELQHFKETTLNHAIL
                     MGRVTFDGIGRRLLPQRETLILTRNSEEKIDGVTTFQDVQSVLDWYQAQEKNLYILGG
                     KQIFQAFEPYLDEVIVTQIHARVEGDTYFPEEFDLSLFETVSSKSYTKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(9343..9861)
                     /locus_tag="VW077_001003"
     CDS             complement(9343..9861)
                     /locus_tag="VW077_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9455038.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMRGRGFMVWHPKMDEYMDALDGHLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLIALFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            complement(10360..11865)
                     /gene="lytC"
                     /locus_tag="VW077_001004"
     CDS             complement(10360..11865)
                     /gene="lytC"
                     /locus_tag="VW077_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9455039.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELKRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(11903..12661)
                     /locus_tag="VW077_001005"
     CDS             complement(11903..12661)
                     /locus_tag="VW077_001005"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9455040.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVALYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(12759..13436)
                     /locus_tag="VW077_001006"
     CDS             complement(12759..13436)
                     /locus_tag="VW077_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9455041.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            complement(13445..14389)
                     /locus_tag="VW077_001007"
     CDS             complement(13445..14389)
                     /locus_tag="VW077_001007"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9455042.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFAWRKIEDDASAYGYL"
     gene            complement(14571..15083)
                     /locus_tag="VW077_001008"
     CDS             complement(14571..15083)
                     /locus_tag="VW077_001008"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9455043.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(15170..15928)
                     /locus_tag="VW077_001009"
     CDS             complement(15170..15928)
                     /locus_tag="VW077_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9455044.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            16136..16282
                     /locus_tag="VW077_001010"
     CDS             16136..16282
                     /locus_tag="VW077_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455045.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(16429..17559)
                     /gene="ugpC"
                     /locus_tag="VW077_001011"
     CDS             complement(16429..17559)
                     /gene="ugpC"
                     /locus_tag="VW077_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9455046.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(17842..17927)
                     /locus_tag="VW077_001012"
     tRNA            complement(17842..17927)
                     /locus_tag="VW077_001012"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17891..17893),aa:Leu,seq:aag)
     gene            complement(18009..18584)
                     /locus_tag="VW077_001013"
     CDS             complement(18009..18584)
                     /locus_tag="VW077_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9455047.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(18584..19372)
                     /gene="codY"
                     /locus_tag="VW077_001014"
     CDS             complement(18584..19372)
                     /gene="codY"
                     /locus_tag="VW077_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9455048.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
BASE COUNT         5942 a         4278 c         3470 g         5828 t
ORIGIN      
        1 attttagaaa tattttttgt gataccctta gaaaggttga tttaacaatg ttttcaacgc
       61 tagaaaaaat taaagaatta gctaaaaaaa gagggattcc tctaacaaaa ctagaagaaa
      121 gtctaggata tagtaccaat tatttctata ctttaaaaac gaaaacccca aactctgaaa
      181 gattgcaaca gatcgctgac tacttcaacg tatctacaga ctacttactt ggtcgtactg
      241 ataatcctgc tattgctggt gatgatacaa tctcaggata cacgtctgac gacctccgaa
      301 agatggcaga gaatgccaaa accttcgatg gtaagccact tacagaagag gatatcgatg
      361 cgattcaaaa catcatagaa atatacttga gaggtagata gtatgacgat taccgaacaa
      421 gatgccttgc ttttcaatac cctttttgaa aacatatatc atcaactccc gtctgttgat
      481 agttctaaag attattggtt tgtaagggct caaaaagggc aattctataa aagttttcaa
      541 actggtggct atatcgccat tggatggaac tatataacat tagatgacct taaaaatctt
      601 gacgaagatg caatcaaagg aaaaatcaag gagtttgaca gtaggataga aaagcctggt
      661 tctgcctaca atcaaatgat gaagtttgcg tatagtctta aaatcggaga cattgtcatt
      721 gttccgtcag aatctcctaa cgattttctt gtaggtgaaa taacgagcag accctataca
      781 gagagtgaaa gcaacataga gtccgcatct aacgtttgcc catttaataa gcgaatgaat
      841 gtgcactggt ttggagtcat accaaacaga gatattgacc cgaaacttta caaattagtc
      901 tattcaggcc ataccatcac tgatgcaaat gcctacaaaa aattcatcaa ccgaggtcta
      961 tatgatgcct acatcgatca tgaccacatg agtatcactt tcaaagtaca agaggaaaac
     1021 aatattgatg cttttgaata ctcaacattt ctgcacactg tcttgcaaat ggtcaatgtt
     1081 gtaaaagaat ctgatgaatt aaaagatgag aaagtcgttc taagaaccaa cgtccaatcg
     1141 aaagggccga ttgaactact tggacatcct gaaatactaa tcccggttct agtatttatc
     1201 attattctaa ctggaggaat tgcttttcgc aatttaatta aacgcaatgg tgcaaacttt
     1261 gaagttgatt ctaagcttgg gaaatttaaa gctcagttaa atagcgatgg ggatgaagca
     1321 atcaaaaaag cccaggcaaa taaaataaat gccgaggcta ttgcattgtt aatagataaa
     1381 gggatgagtc cgcagtttaa aggcgctaca tctcaactag atattaaagc tccagaagta
     1441 gcaactagga ttcttcaaga tgaactactc gaatctccag aaaaagtcga agaataattt
     1501 cagaaataac gaaactgagt aaaacgatga ttggaagtgt taggatttgt cttagaaaaa
     1561 agtaacaaat caaaaaaaca aatgctgaaa cttgaaacag tacagctaaa tgtgcataga
     1621 gtttcaactt cattttaatc acttcctttc aattctattt tactctagtt caagagataa
     1681 aacaactaat tttaaaaaat atttgaagga tgatagccta tgactattga agagctagtt
     1741 gactcacacg gtgtcactct cgcttacttt gataacgaac tttggcaaag acctggagtt
     1801 tatatcaaag aaatcagtat tatctttata aaccgtgaac tgtccgaaga tgcaaaaaaa
     1861 cgggtcatat accacgaatt aggacatctg gaacattcta ctgcactata tcaaaacaac
     1921 cacactaggt gcgagaatga ggctaatagg cacatgattc ataagttgct agaagaagaa
     1981 cttgcactat cagacgacca tcaatctttc aactatatcc agttcatgca aaaacataag
     2041 cttagaacag tcacagacga attgatggta attgacgaat attatgaatt gataggatag
     2101 ttcatcagaa taactataaa aacaaaaaat cctcacactc gccatcgcca aactttgagt
     2161 gtgaggatat ccagtatagt aaaaggcatt aaaaagccct ttttactata cccattttaa
     2221 caaaaaagtg aggtaaacgc aatgtggatg gaagaattgc caaacggcaa atataaattt
     2281 tttgagcgat ataaagatcc atatactgag aaattaaaaa aagtttcagt aaccatggag
     2341 aagaaaactc cccaggcaag aaatcaagct gctatcttgt tacaagagaa gataaataaa
     2401 aaactcagca caaaacaagt agaaagcatt acatttgaag aaatctataa ccttttctat
     2461 aaatcatggg cgcaaacagt aaaggaatca acaaaacata attgtaaatc agttgataag
     2521 aagatgaaag aagtcatacc atccgatacc atacttgcta atcttgacag gcgttttctt
     2581 caagaggcta ttgaaaaaat tattgaaagc aacggatata ttacagctaa aaaagtacgg
     2641 cataggctca gaggtatctt taattacgct gttcaatact cttacattga aaacaacgag
     2701 gtcgattata ctacgattcc tcaaaaacca aagactttag aagaactgga aaaaaagcgt
     2761 aacaactttc tcaccatgca agaaataaaa gcacttgtcg atgtccttaa tcgtcgagaa
     2821 tatcaccaaa agtacgctga tatggttctt gtgctgacat taactggtat gagatatggt
     2881 gagttaactg ccttacaact gaagaatata gacttcgaaa acaacaaaat tgagatcaca
     2941 ggtaattttg attcagtaaa caaaatcaag acgctaccaa agactacaaa ttcaatacgg
     3001 acaatcaaag tatcagagag tgtcatagaa gctattcaaa gacaaatagt acgacttagc
     3061 gaacgtttcc agccattgtc aagcgatgat tatattttct gttttgaaaa atggaatcaa
     3121 cctacaacaa tagcttgctt catacagata ttaaaaaaat atggaaaaca ggccaaaata
     3181 gaaaaaaatt tatctagcca tatttttagg cattctcata tttcgttttt agcagagtct
     3241 ggcctcccaa taaaatcaat aatggatcga gttgggcact caaatgcaaa aatgactttg
     3301 gaaatctatt ctcatactac tgaagatatg gaggataaac tggtcaataa attagatact
     3361 attttttaat tctgcccctt ggttgcccct ttttgatttc ataacacaac aaaacccctt
     3421 gaagatattg ataattcaag gggttgcttt atatcgttat aattcatccg ctatcttatt
     3481 gatttttctg agtctgtgat tgacaccact tttggtcaga ggggtgctga ggctatctgc
     3541 caactgttgg atagagtagt ctgggtgctg aatcctcagt tgcgccactt cctgcaaatc
     3601 tactggcaaa ttttctaagc ccatgatatc ttttatttta ctgatattgt taatggtctt
     3661 catgctggca gaaactgtcc gagcgatatt agctgtctcg gcattattag cccgattgag
     3721 gtcgttacgg gtttctcgca aaatcttaac ccgctcaaaa tcatcacgtg cctgcatggc
     3781 tccgattact atcaagaagt ccataatgtc ttctgctcgc tggagatagg tcacagcccc
     3841 cttcttgcgc tcaagcacct tggcatccag taaaaactgt tggagaaggg aggcaattcc
     3901 ttgcgcgtgg tccagataaa cagaactgat ttccaactgg tacttgcctg actcagggtc
     3961 acgaatgctc ccatttgcca agaaagcgcc acagagatag gcacgacctg cttcctcatc
     4021 cgataaaatc gcctcatcaa tacctgtttc caggccaaag aaagagtctg ccaagtgcaa
     4081 atcacttaac aaatcctgca ccttttcatc tgtaaaaacg gtatagacgc gattcttgcg
     4141 aagattgctc cgttggtggt gacgaatttc agatttgatt tcatagaaat ggagaaagga
     4201 ctcatagagg tgacgggcca gtttggcatt ttctgtcaca actgacaaag tcaagcccga
     4261 agtcgagaga ccgatgctac cagacatttt gataatggca gataattcat gccagctcag
     4321 atggtgttgg cccaggattt cttcttttac tgctactgtg aaactcattt tttcacctgt
     4381 ataatgcgca tcaactcgtc cacaatcaaa tctccatcgt ggaaggcacc gccattttcc
     4441 agacgaagga agttagatga aatcacgcgc gaaacttgct tacaaagacc tacaaaatcg
     4501 tgttccactt gcaccaagta ttcatcaaaa cggttggaat tcatgtattc ctgaggcact
     4561 ttttcaatat tcaccaagac agtgtcgata aaagggcgac caaggtgacg atgcaagact
     4621 tccacgtggt cgctatctgt aaagtgttcc gtctccccac gttgggtcat gatattgcag
     4681 acataggcaa tttctgcctt ggtttccaaa agagcccgcc caatttcctt aatcacgata
     4741 ttgggcaaaa tagaggtaaa gagagaacct ggccctagga caatcatgtc actttcaagg
     4801 atggtctgca ctactcgacg gctggccaga ggcgtatcat cgtttagggc attggtcaca
     4861 tagacattgt caattatgcc tcgatggtct acaatatgac tctctccagc cacttctgtc
     4921 ccatcctgaa agactgcatg aagggtcaaa ggatggtcac tggaaggata aattttccct
     4981 gttgtatgga aaaatttgct caataactgc atggcattat aggttgaacc ctgcatttct
     5041 gacaagccag caatgatgag atttcccaat ggatggccag caaaggctcc ggcatcctca
     5101 gagaaccgat actgaaagac cttctcataa aacttaggca tatccgacat ggccacaagg
     5161 acattacgaa gatcacctgg cggtgtcaac tgttgcatat tttttcggag ttcacctgaa
     5221 gaaccaccat catctgccac cgtcacgata gctgcgattt ccacatcttt ttcccgcaga
     5281 ctttttagaa tgacgggaat tccagtccct ccaccaatca ccgttatctt tggttttctc
     5341 atgaacggtt taccgtttcc tttctgcggt ctttgtcgcg atgcccttca ttaacagacc
     5401 aattcttgga taagtcctgc accaagcgtt tagcaaatgc cacactacgg tgttgtccac
     5461 ccgtacatcc catggcaatg gtcaaaacgg acttaccttc cttttggtaa cttggcagaa
     5521 tcggctcaat caaggccaat aaatgttgat aaaagtcttc tgactcagga tggttcatga
     5581 cataatcata aacaggttca tccacacccg tttggtttct cagttctggt agataatagg
     5641 gatttggcaa gaaacggaca tcaaagacca agtccgcatc aatcgggatt ccatacttaa
     5701 atccgaaaga catgacttcg atacggaaag actgggcttg ttcttggtct gaaaactgct
     5761 ctgcaagggt tttgcgcagc tcacgtggag tgagttcagt cgtatccacc acattttggc
     5821 tcatattttt caaaggtgcc aagagttcac gttccaactt gattccatct aaaatacgac
     5881 cgtctgctgc tagtgggtga ctccgtctgg tctccttgta acgagcgacc aattccttat
     5941 cagccgcatc caaaaagagg attttgaaat ccaaaccatc ttgattttcc aactcatcca
     6001 aaacagcttg aatctctgaa aagaaagaac ggctacgcat atccactacc aaggccaact
     6061 taggattgtc ttccttaatt tcaaccagct gcaaaaactt aggcaagaga gctggcggca
     6121 tattatcaat ggtgaaataa cctagatcct cgaaggactg aatggctaca gttttccctg
     6181 cgccactcat ccctgtcaca atcaccaagt gaagttgttt ctttgtcatc tttttctcct
     6241 tatatcaaaa gaagtttggc aacaccaaac ttcaactagc ttatccaatc tctgcgatga
     6301 cttcaatttc gacttttaca tcacgaggaa gacgagctac ctccacagct gaacgagctg
     6361 ggaattcctc tttgaaggcc gtttggtaaa cctcattaaa aggaacaaag tcgttcatat
     6421 cgctcaagaa acaagttgtt ttgacaacat ggtcaaagtc tgttcctgct tctgccaaaa
     6481 tagcaccgat gtttttcaag acttgctctg tctgttcttg gatattctct cctacaattt
     6541 ccccagtttc aggggatagg ggaacttgac cgctagcaaa caaaaggttg ccaacgattt
     6601 ttccttgaac atagggcccg atagcctttg gggccttatc tgtatgaatt gtttttgcca
     6661 ttttcttttc ctcacaattt ttctaagatt gcatcccaag cctcatccat ccctgccttg
     6721 ctgacagatg aaaagaggat gaaatcgtca cttgggtcaa agtttaattt ctttttgatt
     6781 gctgattcat gcttgttcca tttaccacga ggaatcttgt ccgccttggt cgccacaatg
     6841 atgactggaa tctcataata cttgagaaat tcgtacatct gcacatcatc tgctgacggg
     6901 tcatgacgaa ggtcaactag actgacaacc gcacggagat tttcccgagt cgttaagtac
     6961 tcctcaatca tgcaccccca cttttcacgt tcctttttag aaacacgagc atagccataa
     7021 ccaggcacat ccacaaagcg catcttgtca tcaatgttaa aaaagttcag gagctgggtt
     7081 ttaccaggtt ttcctgatgt acgagcgaga ttcttacggt tcaacatagt gttgataaag
     7141 ctggatttac caacatttga acgccctgct agggcaatct ctggcagttc atcctgcgga
     7201 tagtgggact tattagctgc actgagcaag atttcagcat tgtgtgtatt aagttccata
     7261 gtcacctcta ggctgtttct aggatcggtt tatccgttcc atcgacagtt tctttagtga
     7321 tgcgaaccaa tttcacattt tcctgactcg gcacctcaaa cataacatct agcatggttt
     7381 cttcgatgat ggagcgaagt ccacgcgccc ctgtcttccg ttcgattgct ttattagcaa
     7441 tctcttgaag ggcttcgtcg tcaaattcca actcaacatc atcataagaa agcaaggttt
     7501 ggtattgttt caccaaggca tttcttggct ctttcaagat gcgaaccaag tcatcaacgg
     7561 tcaattgctc aagagccgca aaaacaggca agcgtccaat taactcaggg ataataccaa
     7621 atttttgaat gtcttcagcg atgatttctt gcatgtatga gctgttttcg tcaatcgcct
     7681 tattgttttg accaaatccg atgacttttt cacccagacg ttgtttgaca atttcttcaa
     7741 taccatcaaa agcaccaccc acgatgaaga ggatattttt tgtatccact tgaatcatct
     7801 cttgttgtgg atgtttgcgt ccaccttgag gcggtacgct agcaacagtt ccctcgataa
     7861 tcttgagaag ggcttgttgc accccttcac cagaaacatc acgtgtgata gacacattct
     7921 cactcttctt ggcaatcttg tcaatttcat ccacatagat aatgccacgc tctgcacgtt
     7981 cgatgttaaa gtcagcaacc tgcaagagtt tgaggagtat attttccaca tcctcaccca
     8041 cataaccagc ctcggtaaga gctgtcgcat ccgcaatcgc aaaaggcaca tttaagctct
     8101 tagccaaggt ctgagcaagg aaagttttcc ctgaaccggt tgggccaatc atcaagatgt
     8161 ttgacttctg caaatctaca tcttccgact cttcacgcgt atcgtggaaa ttgatgcgtt
     8221 tgtagtgatt gtaaactgcc actgccaagg cacgcttggc acgatcttga ccgattacat
     8281 agtggttcaa gatatggagg agttcaattg gttttggtac ctcagacaag tctgccaaga
     8341 cttcttccgc caactcttcc cgaatgattt cctgggctaa ctctacacat tcattacaga
     8401 taaaggcgtt gttcccagcg attatttttt gtacttcttc ttggcttttg ccacaaaatg
     8461 agcaataaac catcatatca ttattcctag ttgtaggcat gatttccttc cgttctattc
     8521 tatactgtca ttctatctaa aataaggtca tgtaaaaagc atggatacta ttgaccaaat
     8581 tggtaaaggc atttaaccag agcaggacag aaagtccata acgcttttta cgaaaagcct
     8641 gtgctcctgc aagcaagcag accaaacaca gcatggctgt gaaaaaacca aatatactac
     8701 gttccattag acttcctttc tcttgcggta ttggatggta aaatcataag gatttttctc
     8761 atctttggta taggatttgc ttgaaactgt ctcaaaaaga gacaaatcaa actcctcagg
     8821 gaaataggta tctccctcca cccgagcatg aatttgagtc acaatcactt cgtcaaggta
     8881 gggttcaaaa gcctgaaaaa tttgcttccc accgagaata taaagattct tttcttgagc
     8941 ctgataccag tccaagacag actggacatc ctgaaaagta gtaaccccgt ctatcttttc
     9001 ttcagaatta cgcgtcaaaa tcagagtttc ccgttgtgga agcaagcgac gccctatccc
     9061 atcaaaggtc acgcgcccca tcaagatagc atgattcaga gttgtttctt tgaagtgttg
     9121 cagttctgct ggtaaatacc aaggcagacg attgtcctta ccaatcacac cctcttcatc
     9181 ctgggcccaa atagctacaa ttttcttagt catgcttcca tccttttcac tgatagtact
     9241 attctatcaa aaaactcaaa aaaagactgg tttggaatag cttacaaaat agaaaaaatc
     9301 tgtaagaaat ttcctacaga tttatctatg ttgccttgtt tcttataaac caggtgcttg
     9361 tccaagttct gccgcaagca tccaaactgt tttctcaagt tcagccttag caccaacaaa
     9421 aatatcgttt gtaacatcat cgccttcttc gtcagtcaca tccaacgctt tttggaaaag
     9481 agcgatgagg tagcgataaa tagctagaac acgttccaaa ctttcttcaa cattacggta
     9541 ttcaccagct tcttcttcga tttcactgtt ttgaaggaac tctgtcaatg tagagaatgg
     9601 gctacctcca agtgtaatca atcgttcgct gatttcatct aaatgaccat caagagcatc
     9661 catgtactca tccatttttg gatgccatac cataaaaccg cgtccacgca tataccagtg
     9721 tacttggtgc aaagcaacgt gggctacgta caaatcagca acagcttggt tcaatacttc
     9781 ctttgttttt gccaatgcta ctggcgctgc ttttgttaat gttgcgacat cttttgctgc
     9841 ttcttttttc aattctacca ttttatttta cctcattcat tatttttatg taaccacttc
     9901 ttaatgatta ctaccttagt atactactaa ggaaaaccaa tagcaagaca aagaacttat
     9961 atgagaaaag ttgcaataga ctgataattt aagaattctt tagaagacaa aaaagagggt
    10021 tggatgctta tagtcaatgg aaatccatca gcaaactagg aaactaaacc cagcttgacc
    10081 aaagcacaag ttttgaggtc gcaggtagaa ctgaccaagc tgatgtggtt tgaagaaatt
    10141 ccccaatact ataatagatt gaaataagat gaaaccaagt cgattaggaa aggtaaatga
    10201 atttctaaaa tgttttacca atcacaaggt ctttatctat attcaatcta ctatataagc
    10261 ttcatttccg agcaagatct tgaatttact cgaggatgaa ctatcataaa acaaagaaaa
    10321 agagggtgct actatcacat ccctctttca aatcatcgct taataccaaa cgctgacatc
    10381 tacgcgccct tggattcctt tcatgtattc agaagaggta tattgccaac ctttttctcc
    10441 tgaataataa gggttttccc attctaaagc attcgtatag gccgctaccc agtttacatg
    10501 ttttaaaata tctgggtgtt ttaaacgcgt ctgtaataaa ctacgatagc tatagacata
    10561 cacattttga taacccgcct gcttcatcgt gtccatgtac ttgttgatga ttttaaccca
    10621 agtatctgta tcacttggag ctctcttgct cttatttaca tattcccagt tctcaacatc
    10681 ataatagata gggtaagaca ggttcatatt gtatttcttt ataagttcaa tggtctgttt
    10741 agcgtcattc tcagcatcgg tctcattttc agcataggta tagagataga caccataagg
    10801 aattcccaga cgttttaact ccttaatgtt atgcgccaat tccttgtctt ctttaccgct
    10861 ataacctaga cgaacaatga caccatccac ctcgttctca tcaataacct ttttccaatc
    10921 attgatacga ccattgtgct cactaatatc aatgattttc ttagcatgtt cggttcccac
    10981 ttgttcttct ctattattaa agaaatagcg tttaccatct ctgtgaaccc agccgacatt
    11041 caaatctttc ttttctttga gctcaccaga ggccgcaaag atatagcgag caccatccat
    11101 aatcacttcg tcagtcgcca tagcaccact tccagtcaaa tagtagttgc cttgccactc
    11161 attccgagcc atatagcccc acttcttgaa atagtaccat ttgccgccca ctttttgcca
    11221 ctcttggtta gcataagttc catcggattt tagataaaaa taagcagaat aggctggatc
    11281 atagagccat tcattttgca tcatagctcc ttgaccattc aagaaataac ttccttgcca
    11341 ttggctttta gccatgtaac cccacttctt gaagtagtac cacttatttc caatcaattg
    11401 ccattcttga tgagcataag ccccatctga cttgagataa aaccaactct tgtaattact
    11461 gtcatagatc cactcgtttt gggccatata tccacctgat ttcaggtagt aatttccatg
    11521 ccattcattc tgcgagtaac gaccatctga attaatataa aaccaagcct tatagtagtt
    11581 gtcaaaaatc cattcacttt gagctttaga accatcatcc tttacataat aatccccatc
    11641 ccagtgggca gcagcattag tctgtacttc tgcgctcgtt tgcgttttat tagaagactg
    11701 attttgctct gaactacttg aagctgtcgt tgtatcctgc gaagtttttg ctacttcagt
    11761 ttcatttgca gcgacatgac tagttgccaa gcctagtaaa cagatacttg ctaatccaat
    11821 ttttgttttc aatctttgct ctcctataaa aaatgtaaca gacatctgaa tgctgttcca
    11881 cctagctttt gctacttact gattatttta caaagtcaag caaagccaag aagctttcag
    11941 cttcaagtga cgcaccacct acaagggcac cgtcaacgtc tgggcaagcc atgtataaag
    12001 caacattttc aggtttaaca gaaccaccgt attgaacacg aactttgtct gcgacttctt
    12061 gaccaaagtc agcagctaca acgtcacgaa caactttaca cattttttgt gcatcgtctt
    12121 gtgaagctga tttaccagta ccgatagccc agattggctc ataagcgata actgaggcag
    12181 caacttgttc agcagtcaat ccagccaatg cagcagatac ttgagcacct acgaattcag
    12241 cagctttacc agcttcgtaa gtttcaagtg attcaccaca acagatgatt ggaagcatac
    12301 cgttcgcaaa gattgctttt gcttttttgt tgatatcttc gtcagtttca tggaagtagt
    12361 cacggcgttc tgagtgaccg ataacaacgt agtcagtacc gatttctttc aaaacttgtg
    12421 ggctagtttc accagtgaaa gcacctgcat tttcaaagta gcagttttga gcagcaactt
    12481 taaggtttga gccttttgca acagcaagaa cagttgtcaa atcaagagct ggagcagcga
    12541 tacctgcttc aacaagatct gatgaaggaa gttttgatgc aactgcttca acgaatgctt
    12601 tagcttcttc tggatttttg ttcattttcc agttaccagc gataaatggt ttacgtgaca
    12661 tttcacatac ctcttttttc aatttatttt ctatttattc tatcacaatt atacacagct
    12721 tgcaaatctt actcggattt caagcctact tgcatggatt aatccttcca gagatccatg
    12781 gcatttctga aatctgcaga tacctgtgtc aactgaggat tgcttgcttc tctttctgct
    12841 ctcttggcct caatttgagc cacactcttg attccttcat ggcgccagtt tctcaaaatc
    12901 gcctgaatgt acttccagtt tggttttcca ttcaaaacag cttcacgaag agcctcctta
    12961 atcaagtcag cactggttcc atcttccttt agtgtcttgg tcaaatcctc aatctcaaaa
    13021 ggcgtcaaca atcgtcccaa ctcctgctgg aaggtttcca ccaaatcctt gagctggttt
    13081 tgaggtgtta gctggtctga acttgaatga actgctccaa acaggtcatc caaacgttcc
    13141 aaggccaaac tagcatcaaa gagcaattca atttcgccat ttaattcgat agtacgatac
    13201 tggagcagtc ccctttccgt cagattagaa atggactggt tgacatccga aatttccttg
    13261 ccaatccttt cagcaatctg gcttggcgac atttcttcta agcctgtcgt attttgcaaa
    13321 tagaaaaatt gccaaaccag aaaatcgtcg ctagaaggaa agagttcctt aaaatgcaag
    13381 agcagggcac tcggtaaaac caaggtacct gatttaaaag cgtctaaata tgtcataatt
    13441 cctcttataa atacccatat gcagatgcat catcttctat ctttctccaa gcaaaaggcg
    13501 tctcctgata gaccgcatgg tttacccaat tactgaaaaa gagggctgca gatgaagacc
    13561 aacaaagaca aggtacctga ttaacatcat catctttaaa gtaattttct ggaatatgtg
    13621 ggtcgaaacc tgcatcacga tctcgaaaat actcttttgc caaggtatca cggtcatact
    13681 ccaaatgacc aaaactataa atttctcgta aatcacgact ggccaaaata gaaaccccaa
    13741 cctgaggtcc ttctgataaa atctcgagat tggtcttgtt taagacctct tccttagaaa
    13801 tctccgtgtg ccgtgaatga ggggatacat agctatcatc aaagcctcta aatagaaggt
    13861 gaccctcttt tagggtgtcc tgaggataaa tacctgatag cttactgtcc atctggtatt
    13921 tttctacacc atagcgcaga taaagcccag cctgagcccc ccaacagata tgaagggtcg
    13981 aatagacatg agtcttagac cactcaagca tctgtctaaa ttcctcccaa tagtccactt
    14041 cctcaaatgg taaatgctca actggagcac ccgtgatgat catcccatca aaatactcat
    14101 ccttgacttc aggaaaagtt ttatagaagg tctccatgtg ctctgaacga gttgttttag
    14161 aacggtggct ctccatatag agaaaatcaa tatccagttg taggggtgta ttagccaagt
    14221 ggcgcaacaa ctgggtctct gtgaccattt tctgtggcat gagatttaaa attaaaatct
    14281 tcaaaggacg gatatcttgg tgggcagcac gttgatcatc catgacaaag atattctctg
    14341 tccgtaaaat ctcaacagct ggcaattttt tatcaattcg aatcggcata accctctcct
    14401 aactgtactc tttcaggaat aattgcatat gtttgaagac aaatctcaaa cacttagtcc
    14461 ttttattata ctgtaagaag atatagtttt caattatagt ttttctctaa ctagttatag
    14521 tctattttta tatcctagtg tagagaaaac agccctaggg actgttttca ttaataatgc
    14581 ataagaactt tgtagtcgta gtcaccaatt ttttcacggc cgttcaattc atccaattca
    14641 acaaggaagg cacaacctgc cataacacca ccaagttttt caatcatctc gatagttgcc
    14701 ttaacagttc cacctgtcgc caaaaggtca tctacaataa gaacacgttg acctggctta
    14761 atggcatccg cgtgcatagt caaggtatcg acaccgtact ctttttcata gtcagcagaa
    14821 ataacttcgc gtggcaattt acctggctta cgaacaggcg caaaaccaat tcccaactca
    14881 aaggcaactg gacaacccac gataaatcca cgagcttcag gtcccacgat catgtcgact
    14941 ttcttgtcag tagcatactg aacgatttca cgaacagcgt agctataagc atttccatca
    15001 gccatcaaag gactaatatc acggaaggta atgccttcct ttggataatt ttcaattgtt
    15061 gcaatgtaat cttttaaatt catctttttc tttctttcaa agttttttac tctctattat
    15121 agcatatttt ttaagaaaga aaaaaggaaa agttaacttc aataattatc taacgttttg
    15181 acgatttata actagccatc gcaataaagc ccaatttctg tttattctta gcaaacattt
    15241 tatacatagt taaaaactgc tttctattct cctttttaca agcatttaca caaattttca
    15301 aagttcctag caaaccttcg tcataaatca tacccgataa tttcattaat gtcatttcac
    15361 cagtcaatgc tttcacatca caataacctg attctatcat cacctgttcc caaccatctt
    15421 gagttaaagg acctacattt acatgaattg cttgtgataa ttcctgtctg atagactctt
    15481 tagcttcctt aagaagcaca tcatgtgtca agagaagacc tccaggtttt aataccctta
    15541 gatattccat tacacatttt ttcttagctt gatcggcttg catagtcagc atagcttcat
    15601 ttataacaat atcaaaacta gcatcttgat aaggaagttt cattgcattt gctctttcaa
    15661 aactgattaa atgagcaaca cctgccgttc cagcagattt tttagccact tctaaagctt
    15721 gagcatccat atcaacagca gttatcttgc aaccaaaacg ctgtgccaac tcaattgctg
    15781 tagttcccct attacacgca acctctagta ttctcttttc ttttgaaaat cctccttctg
    15841 caattaacca atctgtggca cgctttccac ctggacgtaa gcgttttttc cccaattttg
    15901 ctaaaaactt atgacctgct tctgacattt gaacacctct gttttttctt cattataaca
    15961 caaaatgata ttatctgaca attctaatct attatttgat aaactagaat caaaagcata
    16021 caagtcacca taactatagc ataaatttat tattacctct ctagttcgaa ttcaattgct
    16081 cacgtagttt acgcatatag cttcacttgt agatggtacc cacaaataat actcaatgga
    16141 aatcaaagag caaactagga aactagccgt aagctgctca aagtacatct ttgaggttgc
    16201 agataaaact gacgaaatca gtaatcatac gtacggcaag gcgacgttga cgtggtttga
    16261 agagattttc gaagagtatt aaagagcatc acaatataga tgcataatat gacttctcta
    16321 gcaaaaagga gagaagcatt ttgtttctct ccctagatta actgatatct ctccactaca
    16381 attgataaag agatatcttt tcttgtagtg ctttgaattt tatttatttt agtagattgt
    16441 tttttcagtt tctacatcga agaagtgtgc tttgttcaag tcaaatccaa gctcaactgt
    16501 tgcacctgtt tgcaagtagt cacgagcatc aacttttgca acaaactcgt ctttaccaac
    16561 ttggcagtaa aggtgagatt ctgaaccaag cagttctgat acagagatag tcgcttttac
    16621 aacacagtct gggaatgttt caaggaaagc aggttctgca ttcacgtctt ctggacggat
    16681 accaaagatc aattcttttc cttcgtagcc tttttcacga agaactttca atgctccttc
    16741 tggcactttc aaacggaaac cgtcagaaac aatttcgcta ccaaccaatt tcacggtgat
    16801 gaagttcata gctgggcttc cgatgaatcc tgcaacgaat ttgttaactg gatttttgta
    16861 aacttcttga ggagtaccga tttgttctac acgtccgata gtacctgtac cagcagggtt
    16921 cttagtagct gacataataa cgatacggtc tgcaagtgtc atcgcttctg tttggtcgtg
    16981 agttacatag atagttgtag ctccgatacg acggtgaatt ttagcgattt cagcacgcat
    17041 tgatacacga agtttggcat ccaagtttga caaaggttcg tccatcaaga atactttcgc
    17101 atcacggaca atcgcacgcc ccatggcaac acgttgacgt tgaccacctg aaaggtcagc
    17161 tggtttacgt tccaagaatt ctttcaatcc aagtatttca gctgcttctt gaacacgttt
    17221 gttaatgtct tctttgctgt atttacgcaa tttcaaaccg aaagccatgt tgtcataaac
    17281 agtcatgtgt gggtaaagag cgtagttttg gaataccatg gcgatatcac ggtcttttgg
    17341 agctacgtcg ttgacaacta cgccatcgat agatgcagta ccttctgtaa tgtcttcaag
    17401 accagcaatc atacggagtg tagttgattt accacatcct gaaggtccta cgaaaacgat
    17461 aaattcttta tctttgatgt tcaagttgaa atcttcaact gaatagtgtt cgctgtttgg
    17521 atattttttg taaatatttt taagattcaa ttctaccatg aggtgaactc cttttgtctt
    17581 ttgataatta tattatatat gaaaacgctt tatatttcta tggcaagatg accaaaaaaa
    17641 taaacaagtt ctgtgcaact tgcacaaaac tttagagaat atcggagaat ttgaagaaga
    17701 taatctttat tattcttcta tgacaaaagc agaaacaagg atcagttttc ccatattttc
    17761 gctgattctc cactacattt gactggattc taatttttta gagaaataca aaagagctag
    17821 ctttagctag ctcttttcct atgcggagag agggacttga accctcacga cctaaagcgg
    17881 tcacaggatc cttagtcctg cgcgtctgcc aattccgcca tccccgcgtc gattacttta
    17941 ctagtatatc aacttttggg atgcttgtca acactttttt tcaaattttt tcattttcac
    18001 caaccaggtt actcagaaag ttcatttaga ttttcatcta ctaacttagc tccgagtgta
    18061 tttttgaaat gacctagggc aaattgatga ttttcaggcc agatggaagc aacagctggt
    18121 ttaacaatct cgatgtcata tcctagatta taggcatcta tagctgtatg taggacacag
    18181 atatccgtca agacacctgt taagataacg gtagacactc tacgctctct caaacgaata
    18241 tctaggtcag tccctgaaaa agctgagtaa tggcgtttat ccatccaaaa gacacgactg
    18301 tctgaaccat gctcttgata aaagatcccc aaatctccat ataaattccg tccactcgtc
    18361 ccaatcagat tatgaggagg aaataactta ctttccggat ggaaacaatc gttttcttca
    18421 tgagcatcaa tagtaaagaa gatataatct cctcgttcaa aagctaatcg agttaccttg
    18481 ctgatggcat ccgaaatcgc ctgagctgga gcacctgctg ttagtttccc actatcagca
    18541 acaaaatctt ctgtataatc aatcgaaatt aaagcctttg tcattagtaa tctcttttct
    18601 tcacttcttc aaaaatatct gaaatcaaga ccttaagata ggttcccttc attccaagtg
    18661 agcgactttc aataatcccc gcagactcaa gtttacgaag agcattgaca atcacagagc
    18721 gagtgattcc gatacgatct gcaatcactg acgcagtcaa cttcccttca tttccattta
    18781 attcccctaa aattgctgaa acagcacgga gttcggagta agaaagggta ttgaccgcca
    18841 tggtgacagc agtacgacga cgaatatttt tctcatcttc ttcacgttgg aagttaagaa
    18901 gctgaatccc aacaacggta ctggcaatct caacaagaac caagtcctca tcttcgaatt
    18961 ttttatcatt acgccaaata atcaaagaac caaggcgaat ccccgataca tgaatcggtg
    19021 caatagtcgt caagccatct ggaaaatcat ctctactctc aacagggaaa atactcatat
    19081 catgctcaac aggtaagttt gcttctgttt cgtaaatcat attagcccct tgaacgtagt
    19141 catctgggaa aatcttagtt tggaagaatt gctctacgcg atctgtattt gttttataac
    19201 gcataaaata gccaagcaga cgtcccttac tattgataat gcaggcattg caatgaataa
    19261 tatccgctaa ctgacgcgta atagcgttgt aaggaagctc atcctgcaac tgctcctctg
    19321 agcgtttcaa aattgatgta atttttctag ttttttctaa taaatgtgcc atttttcacc
    19381 tcgaatttaa tcgctatcat tataacataa aaacgtctct ttttcaataa ttatctgaaa
    19441 attccttatt gacttgcatt gacttacaat ttaattaaaa accagaatat ttttaattaa
    19501 attgttcctt ttctattg
//
LOCUS       DAVKGU010000029        19456 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKGU010000029 DAVKGU010000000
VERSION     DAVKGU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19456
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.029"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(24..3200)
                     /gene="carB"
                     /locus_tag="VW077_001015"
     CDS             complement(24..3200)
                     /gene="carB"
                     /locus_tag="VW077_001015"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9455049.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIKSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3513..4592)
                     /gene="carA"
                     /locus_tag="VW077_001016"
     CDS             complement(3513..4592)
                     /gene="carA"
                     /locus_tag="VW077_001016"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9455050.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(4642..5565)
                     /locus_tag="VW077_001017"
     CDS             complement(4642..5565)
                     /locus_tag="VW077_001017"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9455051.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5584..6105)
                     /gene="pyrR"
                     /locus_tag="VW077_001018"
     CDS             complement(5584..6105)
                     /gene="pyrR"
                     /locus_tag="VW077_001018"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9455052.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6316..6945)
                     /gene="nth"
                     /locus_tag="VW077_001019"
     CDS             complement(6316..6945)
                     /gene="nth"
                     /locus_tag="VW077_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9455053.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(6945..7487)
                     /locus_tag="VW077_001020"
     CDS             complement(6945..7487)
                     /locus_tag="VW077_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9455054.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            7657..7797
                     /locus_tag="VW077_001021"
     CDS             7657..7797
                     /locus_tag="VW077_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEU9455055.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKQ
                     KS"
     gene            8212..9771
                     /locus_tag="VW077_001022"
     CDS             8212..9771
                     /locus_tag="VW077_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9455056.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRNLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKILHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGSLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGVGKSTLLAKLKQFLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9920..10819)
                     /gene="htpX"
                     /locus_tag="VW077_001023"
     CDS             complement(9920..10819)
                     /gene="htpX"
                     /locus_tag="VW077_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9455057.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(10821..11381)
                     /locus_tag="VW077_001024"
     CDS             complement(10821..11381)
                     /locus_tag="VW077_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9455058.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11475..12188
                     /gene="rsmG"
                     /locus_tag="VW077_001025"
     CDS             11475..12188
                     /gene="rsmG"
                     /locus_tag="VW077_001025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9455059.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12442..13724
                     /locus_tag="VW077_001026"
                     /pseudo
     CDS             12442..13724
                     /locus_tag="VW077_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
     gene            complement(13919..15490)
                     /gene="ffh"
                     /locus_tag="VW077_001027"
     CDS             complement(13919..15490)
                     /gene="ffh"
                     /locus_tag="VW077_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9455060.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVCHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15502..15834)
                     /locus_tag="VW077_001028"
     CDS             complement(15502..15834)
                     /locus_tag="VW077_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9455061.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(15925..16302)
                     /locus_tag="VW077_001029"
     CDS             complement(15925..16302)
                     /locus_tag="VW077_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9455062.1"
                     /translation="MKIRMRNTIQFDEQLEVIDRLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            16374..17678
                     /locus_tag="VW077_001030"
     CDS             16374..17678
                     /locus_tag="VW077_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9455063.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLAE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSLIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            17691..18497
                     /gene="yidA"
                     /locus_tag="VW077_001031"
     CDS             17691..18497
                     /gene="yidA"
                     /locus_tag="VW077_001031"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9455064.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            18540..>19456
                     /locus_tag="VW077_001032"
     CDS             18540..>19456
                     /locus_tag="VW077_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HEU9455065.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDITMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRST"
BASE COUNT         5754 a         4319 c         3705 g         5678 t
ORIGIN      
        1 tctgctatat cttatttttc tatttaaatc gcttctgtaa caaagctacg actttcaagt
       61 accttaagca tggcattagc tgtatctagc gctgtgaaga ggggcacccc gtgttcaatg
      121 gctgaacgac gaatttgctc accatcttcg tcagcagttc gttttgttcc tactgtgtta
      181 atgatagctt gaattcttcc tttgcgtaca aaacttggga tatccttatc gtcatcacca
      241 atcttaccaa caggttgggc ttgcaagcca tgactagcaa agaaggctgc tgtcccttct
      301 gtcgcaagga ttccataacc aatgttttgg aaacgacgag ccaagttcaa ggcttcttct
      361 ttggcatcat cagcgatggt aaagacgaca ttaccaaaag ttggcaagtg tagataagaa
      421 gcttcaaagg ctttatagag agctttttcc aaagtagcat cagaacccat aacttcacct
      481 gttgacttca tttcaggacc gagcaagctg tctaccttag ctagtttggt aaaggagaag
      541 acaggtgcct tgatatgaac acgggtgctt tcagggtaaa gtccattttg gtagccaagt
      601 tctgataaac tttgaccaag aatgagcttg gtcgctactt gagccatagg aatattggtt
      661 accttagata ggaatggaac ggtacggctg gcacgtggat tgacctcaat aacgtagact
      721 ttttcatcct tgataacaaa ctggatgttc atcattccaa ggcagtgaag accgattgct
      781 aagcgtttgg tgtagtctgc gatggtctcc tgaacctttt gcgacaaggt ttgtggtggg
      841 taaacagcca ttgagtcacc tgagtggaca ccagcacgtt cgatatgctc catgatacca
      901 ggaatgagta catttttacc atctgaaatg gcatcaactt cgcactcttg cccaacgata
      961 taagagtcga caagaactgg atggtctgga ctagccttaa cagcagttcg catgtaagaa
     1021 cgaaggtctt cttcgttttc aacgatttcc atggcacgtc caccaagtac ataagatggg
     1081 cggacaagaa ctgggaagcc aatcttacga gctgcaagag ctgcttcttc ttcattggta
     1141 gccgtttgtc ctggtggctg tggaatatcc aattctttga gagcttgctc gaagaggtca
     1201 cggtcttcgg cacggtctag gtcagcaacc tgtgtaccaa ggatggtcac acctgctttt
     1261 gccaatggct ctgcaaggtt gatggctgtt tgaccaccga actgaacgat aactcccttt
     1321 ggttgttcca agtcaatgac gttcataaca tcttcgaatg tcaatggctc aaagtaaagc
     1381 ttatctgata cagagaagtc tgttgaaacg gtctctgggt ttgagttcat gatgatagct
     1441 tcataaccag ctgcctggat agccttaaca gagtgaacgg ttgcgtagtc aaactcaacc
     1501 ccttgaccga tacggattgg acctgaacct aggacaagta cagattcttt atcagatttg
     1561 atagattcat tttcccaacc ataggttgaa tagaaatatg gcgtttcgga atcgaactct
     1621 gccgcacaag tgtctaccat cttataaact ggaacaatct tgttttccaa gcgaagttgg
     1681 cgaactttat catcagtcgt tccccagagt tcagcaatct tacggtctga aaaaccatta
     1741 agtttggctg ttttcaaaac ttctagatct tgtggatgag cacccaattc ttgctcaatt
     1801 tcaaagatat gcaagagttt atcaagatag aagatatcaa tttttgtaag ctctgcaatt
     1861 tcttcaggtg tgtagccacg acgaatggct tctgatacgt agaagagacg gtcatcttgg
     1921 gctttgacaa ccttttcaat caaggtatca tcagaaactg ctgcaagttc aggtatttca
     1981 ttgtggtgca ccccaatttc aagggagcgg caggccttga gaagagattc ctcgatgtta
     2041 cgaccgattg ccatgacttc tccagtcgcc ttcatttgtg taccgagacg gcgttcaccc
     2101 ttttcaaact tgtcaaatgg gaaacgtgga atcttagcaa ctacgtagtc aagggctggt
     2161 tcaaacatgg cataggttga acctgtaact gggtttataa cctcatccaa ggtcaaacct
     2221 actgcaatct tggcagccaa cttagcaatc ggatatcctg tcgctttaga agcaagggct
     2281 gacgaacgtg atacacgagg gtttacttcg ataacataat acttgaagct gttaggatca
     2341 agagctagct gaacattaca tccaccttca atcttgaggg cacgaataat gctcaagctc
     2401 gcatcacgaa gcatttggtt ttcatagtct gacatggttt gcgcaggggc aaatacaatg
     2461 gaatcccctg tgtgaatccc aactgggtca aagttttcca tgttacaaac aaccaaggca
     2521 ttgtcagctg agtcacgcat cacttcgtat tcaatttcct tgaaaccggc aatcgaacgc
     2581 tcaatcaaac attgggtaac aggtgacaat ttcaaaccat tttcagtgat ttcacgcaat
     2641 tctttctcgt tggcacacat accaccacca gtaccaccaa gggtaaaggc tggacgaacg
     2701 atgactgggt agccaattgt cgctgcaaag gcaactgctt cttctactgt gttaacaatt
     2761 tcagattctg gaatgggttg ttcaagctct tccatcaatt gtttaaagag atcacggtcc
     2821 tccgcttggt caatggcaga taatttggta cccagaagtt caacgccaag ctcgtctagg
     2881 ataccatttt tagataattc catggccatg ttgagacctg tctgaccacc gagtgttggt
     2941 agcaaggcat ctggaccttc cttacgaaga atacgtgtca caaactcaag tgtaatcggt
     3001 tcaatgtaaa ccttgtcagc aatttccttg tccgtcatga tggttgcagg atttgagtta
     3061 accaaaacaa cctcataacc ttcctctttc aacgacaagc aagcctgagt cccagcgtag
     3121 tcaaactcag cagcctgacc aataataatc ggaccagaac caatcaccat aattttttga
     3181 atatcagtac gtttaggcat atataagata ttaagggtgt caagcggaca aagctaaaat
     3241 aggagttatg acgaagaact gtcagttcta ggaataacta tctttttagc accgtccgta
     3301 gcccgtattc agttcagcaa atacggagct cccttctcct ttctattcgt cgcctctcag
     3361 ggcgacatta aataagatac aaaggacgaa tagaaagcga ttgaatttta ggaaatcaag
     3421 gaaggattga caatccaagt tggtttctct acattctgag ctttccgtcc gtgttcagtt
     3481 acataaattc tccgacgagc ttttactcgt tcttagtttg attgtttaaa aacttccatc
     3541 atctcgataa actcgtcaaa taggtagcta gcgtcgtgtg gcccaggagc tgcatctggg
     3601 tggtattgaa cagagaaagc aggttggtat ctgtggcgca caccttccac tgacttgtca
     3661 ttgatttctt cgtgggtaat aatcaagtgc tctggcaaat cctcgcggct gactgcataa
     3721 ccatggttct ggctggtgaa gtctactcgt cctgttgcga tttcacgtac cgcatggttg
     3781 aatccacggt ggccaaactt catcttatag gtcttagccc cgtttgccat tgcaaagagt
     3841 tggtgtccca tacaaatacc aaagattgga atttttcctt gtacaccgcg aatcatgtcg
     3901 agtgcttgtg gaacgtcttc tgggttacct ggaccatttg acaacataac tccgtcagga
     3961 ttgagatgga gaatttcttc agccgttgtc gaataaggaa caactgtcac gttacagttg
     4021 cgtttagaaa gttcacgtag gattgagtgc ttgagaccaa agtccactag caccacgctc
     4081 aaaccaactc ctggagctgg ataagacgtt ttagtagaaa cctgtttgat attgtctgtc
     4141 ggtaaaactg ttgcttggag ctggtccgtc acatggtcca tactgtcccc aacatgggtc
     4201 aaggttgcac gcatagtacc atgcttacgg ataatcttgg taagagcacg cgtatcaatt
     4261 cctgaaatcc ctggaatttt cttggctttc aaaaattcat ccaaggtcat ttggttgcgc
     4321 cagttgctag ctctacgcgc ttcttcaaaa acaacgactc ccttacaagt tggaataatg
     4381 gattcataat catcacgatt aataccataa tttcctacca aaggataagt aaaggtcaag
     4441 atttgtccat tataagactg gtctgtaatg gattcttggt agccggtcat ccctgtatta
     4501 aagacgattt cgcctgttac atcaatatct gctccgaagg ccttgccttc aaaaactgtg
     4561 ccatcttcta atactagaat tctttttgtc atattttcac ctctcgtgga cgctcactgg
     4621 cgtcttttaa catcttgtgt tttagttggc gtttctactc gctagtacgg attctaagat
     4681 tgccattcga acaaagacac cattggtcat ttgttggaca atccgtgatt ttggtgcttc
     4741 aaccaagtgg tctgctattt ctacatcacg attgattgga gctgggtgca tgaggattgc
     4801 tgtttctttc aaacgatcgt aacgttcttg agtcaagcca tgttgggcat ggtagtcttc
     4861 ttttgaaaat acagctccac tatcatggcg ttcgtgttgc acacggagaa acatcatgac
     4921 atcaacctga tcaatgattt catcaatggt tacaaactgt ccatagtctg caaactcttg
     4981 acttctccat tcctcaggtc cagcgaaaaa gagttcagat cccaagcgtt tcaaaatctg
     5041 catattggat ttggcaacgc gtgagtggtc caagtcacct gcaatagcaa ctttaagacc
     5101 ctcaaagtgg ccaaattcct cataaatggt catcaaatca agcaagctct ggctagggtg
     5161 ttggcccgaa ccatctccac cattgatgat ggaagtcgta atcgttggac tagcaatcaa
     5221 ttctctatag tagtcgacct ctggatggcg aatcacacag acatccactc ctaaagcaga
     5281 cagagtcaaa atggtgtcat aaagtgtctc acccttatta accgaactag tcttcacatc
     5341 aaagtcaagt cgttccaatc caagtttaat ctctgcgact tcaaaggact tatgtgtccg
     5401 tgtagaatcc tcaaagaaga gattggaaac aatcggatgg tcttcatagg gaagctgggc
     5461 tccattttta aactcaattc ctcgcttgat caatttcatt acttgatcga cagtgaggtc
     5521 ttccatggac accacatggt tcaatgcttg ttgattttct gacatggcta ctcctttaac
     5581 tttctaagct tcttcagtaa tcagaactct gtcttggtca tcaagttctg tcatctctac
     5641 gatgatttct tcagaacgac tggttgggat attttttcca acgtaatctg gacggattgg
     5701 caattctcta tgtccacgat cgactagaac tgctaaactc acacgcgcag gacgaccatg
     5761 accgacaata ttatcaatag cagcacggat ggtacgacct gtatagagca catcatccac
     5821 caagataact tcgcggtctg tcacatcgac agaaaccaaa gaagtatctt ctccactttt
     5881 aacatcatca cggaaaggtt tagtatccaa ttccacaaca ggaactgaaa gattttctaa
     5941 ctgcttcaaa cgttcttgga ttcggtgggc aataaagaca ccacgagttt taataccagc
     6001 caagacgatc ttattcaaat ctttgttgcg ttcgataatc tcataagtaa tacgcgtaat
     6061 cgctcgtttg acggtcaatt cgtctacaac ttcttttgtt ttcatgacaa acctccaaaa
     6121 agaaaagtct ccttaaacaa ggagacttga aatttatagc caagcgagcc ctactgcaca
     6181 cagtatagac ttcacccttc tactttatcg cgctccttgc ctgcctcacg ggacaggttt
     6241 aaaggaatat ttagttatca tttactatag cacaaagcat gcttaaaatc aagcaaaaag
     6301 tttcaatgta gcatcttaca aattgctaaa atcatataat tgtgggtact ggtcacactc
     6361 tggatttttt ggatggcaaa tggctcttcc aaaataaatc atggcctgat gggcagctaa
     6421 ccactgctca ggcggcaaga tatccatgac ccgcttttcc acctcaagtg gcgtcgctga
     6481 ttttttgaca atatcgtggt gtttgcaaat acgctccaca tgagtatcca ctgcaaaggc
     6541 tggaatccca aatcctacac tcatgacaac attggctgtc ttgcgaccaa cacctgccaa
     6601 actctccaat tcttcacgtg tctgagggac ttgaccatca aaatcgtcta gtaactgttg
     6661 ggcacatttt ttaaggaatt tagctttatt ccgatacaat cccaagcgag aaatatgtga
     6721 agcaatctca ctctctgtcg ctacagacat agcttggggt gttggaaagg caacaaagag
     6781 acctggtgtg gccttattta ccgctgcatc tgtcgtctgg gctgataaca tgaccgcaac
     6841 caggagttca aaatgattgg taaaatcaag actaggcttg gcatctggga agagggcaat
     6901 gatttcttct agcacctttc gtgctcgttt ttttgacaag accattattc atctccgtca
     6961 aatagtcctt gtaagccagc aaaaggactg ttttcttctt tctttactgc ttgttgagct
     7021 tggtattctt cctctgtcat gatttgccag tcatttcctg agacaaatcc ttgaccagct
     7081 tcttcttcag ccgtcaagac cttgatagga atgtttagca agatattgtc tgatacactc
     7141 tcagcaaggt caagcgcccc attttcgatg ggcaagacca agtcatcatc taaaatttct
     7201 tgatttagat ggttagttgc gccttccatg aaaacttccg tgactggata agattcaact
     7261 aactcaactg gctccatact gcgactcgaa gcaagaacaa tggtataaga tagttgataa
     7321 tctaagaaat acatacggtc ttcatattgg actttcccaa ctgcaaggat atcttttaca
     7381 tctaaaattt cttgattacg tgcacgcagg tcatcaacta aatctaacgt ttgttcaaag
     7441 ttcaaacctt cagactgctt acgaatttct tgaatattta atttcatact tcctccataa
     7501 agatttactc tcttgattat accatgaaaa ggctacaaat cagcacacca aactttgtaa
     7561 ttaaaattcg aaattttaac atattcacta tgacattttc attttttagt gctatactat
     7621 agggaaagag tacatcagat caaggaggat gctcacatgg aagacaagaa actcattcaa
     7681 ctcctatcca agttaaataa aagctaccaa aactgtaaac agggtacggc agatgatatt
     7741 cgactacaag agctgctaaa cactactatg caagagctca aaaaacagaa aagttgaaca
     7801 acagtatctt aattgatctt gagaaatttt accaacccac cagtcctctg attggactgg
     7861 gtagcctaaa actaaacaat caagaacgca ctgcttggcg aaactatgat aaaatccatt
     7921 acgagcatgt caaacacgta ctaagtctct atggacctgt ttttggattt tagagcatag
     7981 aatttccagt tttctgttga caaaatttcc ttaaaagtat agtataaaga tactaatact
     8041 cggaggtaag ggagacatga acaactaagt ctatcaaata aagaaccttt atttagtaga
     8101 tcttgttttt gtctcttttt gtgtgctctt ttatgctctt tttctggcat gttaatagag
     8161 tttttttgac atagactttg ggctctacta ggtaaagtag agctttttgt tatgcactat
     8221 cgacattcta gaaagggcaa tagtatgata aaaatcaatc acctgaccat cacacaaaac
     8281 aaagatttac gaaaccttgt ctctgaccta agcatgacca tacaagacgg ggaaaaggta
     8341 gctattattg gcgaagaagg aaatggtaaa tcaaccttac ttaaaatttt aatgggggaa
     8401 gctttgtctg atttcactat caagggaaac atccaatctg actatcagtc actggcctac
     8461 attcctcaaa aacttcccga ggaactgaaa aagaaaattt tacacgacta cttcttttta
     8521 gattctattg atttagacta cagtatcctc tatcgtttgg cggaggaatt gcattttgat
     8581 agcaatcgtt tcgcaagtga ccaagagatt ggcagtctat cagggggcga agctttgaaa
     8641 attcagctta tccatgagtt agccaaaccc tttgagattc tatttttaga tgaaccttca
     8701 aatgacctag accttgagac agttgattgg ttaaaaggcc aaattcaaaa gaccaggcaa
     8761 accgttattt tcatttccca tgatgaagac tttctttctg aaacggcaga cactattgtt
     8821 cacttgcgac tggtcaaaca ccgtaaagaa gcggaaacgc tagtagagca tttagactat
     8881 gatagctata gtgagcagag aaaggctaat tttgccaaac aaagtcagca agctgctaac
     8941 aaccaaagag cctacgataa aaccatggaa aaacatcgga gagttaagca aaatgtagaa
     9001 actgcgcttc gagctaccaa agatagtact gccggtcgcc tattggctaa aaagatgaaa
     9061 actgtcctct cacaagaaaa acgctacgaa aaggcagctc agtccatgac tcaaaagcca
     9121 cttgaagagg aacaaatcca acttttcttt tcagacatcc aaccattacc agcttctaaa
     9181 gtcttagtcc aactggaaaa agaaaatttg tccattgacg accgtgtttt ggctcaggaa
     9241 ctgcaactaa ctgtccgtgg gcaagaaaaa atcggtatta tcggaccaaa tggtgttggg
     9301 aaatcaactc tgttagccaa attaaagcaa tttctgaatg ataaaagaga gatttccctt
     9361 ggttttatgc cacaagatta ccacaaaaaa ctgcaattgg atttatcacc aatagcctac
     9421 ctcagcaaaa ctggggaaaa agaggaacta cagaaaatcc aatctcacct agctagtctc
     9481 aatttcagtt atccagaaat gcagcatcaa attcgctcct tatctggcgg acaacaggga
     9541 aaactcctgc ttttggattt agtcctgcgc aaaccaaact ttctcctgct ggatgaaccc
     9601 acacgaaact tttctcccac ttctcaaccc caaatcagaa aactctttgc cacttatcct
     9661 ggcggtctca tcactgtttc gcatgaccgt cgtttcttaa aagaagtctg ctcgatcatc
     9721 tatcgcatga cagaacacgg tttgaagcta gttaatttag aagatttata aatttgcaac
     9781 atagcagata ggtggggcaa aaacacaatt ttaggagaaa aagaagtaaa tcttcccata
     9841 gtaaaacgca taatatcaag ttttcacacc tgatattatg cgtttttcag attttaaaga
     9901 cttttttcca gccttcattt tacatctgtt ttaaacgttc aatccgttct gagataggtg
     9961 ggtgggtata aaagagtttt tggaaccccc cacctttctt gggatcattg atataaagag
    10021 cactgctagc atcatcgacg tggcgactca taggtttgct attgtccaac ttatctaggg
    10081 cattaatcat tccctgggga ttgcgagtca gctcgacact agatgcatct gccagaaatt
    10141 ccctctgacg agaaatagcg agctgaacca aggttgcagc gagaggtgcc agtacaatag
    10201 ctagtaggga aaccactagc ataatgattt caagaccatt tccatctcgg tcatcatcac
    10261 ttcgtctgcg acctgctcca ccccaccaca tcatacgacc tgccatacta gaaagcatgg
    10321 tgatagcact agcaagagca actgcaatag tcgaaatacg gatatcataa ttacgaatat
    10381 gactgacttc atgtcccata acagcttcta gttcttcacg attcatgata gctagtagac
    10441 ctgaagtcgc agcaacagcc gcattttgag gattagaacc tgtcgcaaag gcatttaagg
    10501 ctggatcatc aatgatgaaa acacggggca taggaatctg agcgaccaga gccatatctt
    10561 ccactacatg gtagaggtct ggtgccgttt gctcatccac ctcacgcgct ccattcatgg
    10621 acatgacaat ctctgtcgat tgaaaaatca tagacaaagc gtagataaag ccgataatca
    10681 gtgcgataac caaaccacca agtccagatc ttataaagag ataaccaacc gcataaccaa
    10741 caagagctaa gagtaggaaa aataccagca acaaaatcca ggtttttcgt ttattgcttg
    10801 caatttgatc aaacaacatc ttagtcacct aaaccgctaa aatcaacttt aggaaccgac
    10861 ttttcctctt caggtgtttg aaggaaatct gccgctttaa atccaaacat tccagcgata
    10921 atattgctcg ggaaagtttc taattttaca ttgtagttgc tgacaacact gttatagagt
    10981 tgacgagagt aagaaatttt attttctgtg tttgtcaact cctcttgcaa tttaacaaag
    11041 ttagcactag ctttcaaatc tggatagctt tctgcaactg caaaaatacc tgaaacttga
    11101 cgagtgaggg catcactggc tttcatagct tctgctggtg aagtcgctgc cgccacttgg
    11161 ttacgtagtt ctgccacctt ttcaagggta gaaccttcat atttggcata accttttaca
    11221 gtctcaatca agtttggcaa gaggtcattt cgacgtttca actgaacatc aatctgactc
    11281 caagcctcct tggtttgcat acgattttta accaaaccgt tatagctaac aatcacaaaa
    11341 ataacaataa gagcgataac tccaagaata atccaagtca tgatataagt cctttctact
    11401 tttagattag taccagtata tcaaattttt aatgattgtg gtaaaataag atgatactaa
    11461 agaaggaaat aactatgaaa ccagaaacat tttacaactt gcttgccgag cagaatcttc
    11521 cactttcgga ccagcaaaaa gaacaatttg aacgttattt tgagctcttg gtcgagtgga
    11581 atgagaagat taatttgacg gcgattacgg acaaggaaga agtttatctc aaacattttt
    11641 acgattcgat tgcacccatt cttcaaggtt tgattcccaa tgaaactatc aaacttcttg
    11701 atatcggggc tggggcagga tttcctagtc taccaatgaa aattctctat ccggagttag
    11761 atgtgaccat tattgattca ctcaataagc gcatcaactt cctacaactc ttggctcaag
    11821 aactggattt gaacggagtt catttctacc acggacgtgc cgaagatttt gcccaagaca
    11881 agaacttccg tgctcaatat gattttgtaa cagctcgtgc ggttgcccgt atgcaggtcc
    11941 tatctgaatt gactattccc taccttaagg ttggtggaaa actattggca ctcaaggcca
    12001 gcaatgcgcc tgaggagttg ttagaagcta agaatgccct caatctcctt tttagtaaag
    12061 tcgaagacaa tctcagctac gccctaccaa atagagatcc gcgttacatc acagtggtag
    12121 aaaagaaaaa agaaacacca aataaatatc cacgtaaggc tggtatgcca aataaacgcc
    12181 cgctttaaat ttttaagtaa aaaaatattt acaaaatcaa cctcgctctc ttatttttag
    12241 gctcgggaaa aaatgattta caaaatcatt tttttctgct atactatcct aagcaaaggt
    12301 ttttaatgtc atcccgtgag gtgacgaaga cgcagaaata tttgaaactc tttaaagtct
    12361 agattttaga gaagtcttac tctgagggcc tattgctgta aaataatggg ctcttttctg
    12421 atgcccaaaa gtgaggttta tatgaaacaa gaatcaactg ttgatttgtt actagacgtt
    12481 gaccaacgtc cttcggatgg aaaaggaatt ctccttagtt tccaacacgt tttcgccatg
    12541 tttggtgcga ccatcttggt accattgatt ttgggaatgc ctgtatctgt tgcccttttt
    12601 gcttcaggtg ttggaacact catctacatg attgctactg gttttaaagt tccagtttat
    12661 ctaggttctt catttgcctt tatcacagct atgtcactgg ctatgaaaga aatggggggg
    12721 atgtatctgc tgcccaaaca ggggttatct tgactggttt ggtctatgtc cttgttgcta
    12781 ccagcatccg atttgtagga acaaaatgga ttgataaact cttgccacca atcattatcg
    12841 gtcctatgat catcgttatc ggtcttggac ttgcaggttc agctgttacc aatgcaggtc
    12901 ttgtagcaga cggaaattgg aaaaatgctc tggtagccgt tgttactttc ctaattgctg
    12961 cctttatcaa tacaaaagga aaaggcttcc tacgaatcat tccattcctc tttgccatta
    13021 tcggtggtta ccttttcgca ctaactcttg gcttggttga ctttacacct gtcctagaag
    13081 ctaactggtt tgaaattcct ggtttctact tgccatttag cacaggtggt gcctttaaag
    13141 agtacaatct ttactttggt ccagaagcca tcgctatctt gccaatcgct atcgtaacaa
    13201 tttctgaaca tatcggagac catactgttt tgggtcaaat ctgtggtcgt caattcttaa
    13261 aagaaccagg tcttcaccgt actcttcttg gtgacggtat cgcaacttct gtttctgcct
    13321 tccttggtgg accagccaat acaacttacg gagaaaatac aggggttatc ggtatgactc
    13381 gtatcgcttc tgtctcagtt atccgtaacg ctgccttcat cgcgattgcc ctcagcttcc
    13441 ttggtaaatt cactgccttg atttcaacta ttccaaacgc tgtacttggt ggtatgtcaa
    13501 tccttctcta tggggttatc gccagcaatg gtttgaaagt cttgattaaa gaacgtgttg
    13561 atttcgctca aatgcgaaac ctcatcatcg caagtgctat gttggttctt ggacttggag
    13621 gagctatcct taaacttggt ccagttacac tttcaggtac tgccctttca gccatgacag
    13681 gaatcatctt gaacttgatc ttgccatacg aaaataaaga ctaagagtct aaatacacct
    13741 aatccactca gacagctgag tggatttttc gtataccata ataaaagtgt cttaacaaaa
    13801 ttattaaaat caaaaaacgt ataatatcag atattctaaa accttgatac tgtacgtttt
    13861 atcatagaaa tttttacttt attttctcat caaatgagat ttgcatcaat ctcttgtctt
    13921 acttgcgttt cttcttcgct ttcttcattt tgttagccat acgtttcatg gactgtttca
    13981 tggcaaattc accaatttta cctttcaaac cgccaccaaa catctggctc atatctggca
    14041 ttcctgctcc tccgagagct gataagtcag gcataccgcc ttgtcccatc attccttcaa
    14101 gggcagacat atccattcct cccatatttg gcatattttt aggaaggtta tttggattaa
    14161 tccccatttg cttcatcatt ttattcatat ccccagacat aacaccctgc atgagctgtt
    14221 tagcctggtt aaagtccttg atgaatttat tgacttcgac gaatgtattt ccagaaccag
    14281 cagcaatacg acggcgacgg cttggattta acaaatctgg gttttcacgt tcttcaggtg
    14341 tcatcgaaga cacaatcgca cgtttacgag caatctggcg ttcatccacc ttcatgtttt
    14401 gaagtgctgg attgttggcc atacctggaa tcatcttgag caagtcttcc atcggcccca
    14461 tattttgcac ctgatctaat tgatcgatga aatcattaaa atcaaaggtg ttttcgcgca
    14521 tcttctcagc catttcaagg gctttttgtt catcgtattc ctgagaagct ttctcaatca
    14581 aagtgagcat atcccccatg ccaaggatac ggctagacat gcggtctggg tggaaggttt
    14641 caatgtccgt aatcttttca cctgtaccag tgaacttgat tggttttcca gtaatgtgac
    14701 aaacagacag agcagcacca ccacgagtat cgccatcaat cttggtaagg atgaccccag
    14761 tcacttccaa ctgagcatta aactcacgcg caacattggc tgcttcctga ccaatcatag
    14821 catcaacgac aagcaagatt tcatttggtt gagccaatgc tttcacatca cgaagctcat
    14881 tcatgaggag ctcatcaatc tgcaaacgac ccgcagtatc aatcaagaca tagtcgttat
    14941 gattagtttg agcttgctcc aaaccttgac gtacaatctc aacagctgga acctctgtcc
    15001 caagagcgaa aaccggcaca tcaatctgtt gtcccagtgt cttcagctga tcaatggcag
    15061 caggacggta aatatccgcc gcaatcatca aaggacgggc attttcttcc ttcttgagtt
    15121 tgttagccag cttacctgca aaagttgttt taccagctcc ctgcaaacca accatcatga
    15181 tgatggttgg aatcttaggt gacttgataa tttctgccgt atcagaacct aaaacggctg
    15241 tcagttcctc atcaacgatt ttaataatct gttgcgcagg attaagtgta tcaatgacct
    15301 catgcccgac tgcacgctcg cgaactttct tgataaagtc ctttacaaca ggcaaggcaa
    15361 cgtcggcctc gagcaaggcc aagcgaattt ctttggttgc ctcttggaca tcagattcag
    15421 agatttttcc ttttttacgt agatttttaa agacgttctg caaacgttct gttaaacttt
    15481 caaatgccat ttttcttcct cttattctct attatcaatg cttgttaaaa tttctatctg
    15541 ctcctgcaga aagtcatcct tgggatagcg ctccaaaatc tgatcaaaaa tctgactgcg
    15601 gacaatatag tccgagtaca tgtgcaattt catctcataa tcttccagaa tcttttctgt
    15661 tcgcttgata ttgtcataga cagcctgacg actgacacca aactcctcgg caatttcagc
    15721 aaggctgtaa tcatcagcgt agtagagctc gatataattc atttgcttat ctgtcaaaag
    15781 cgccgcataa aattcaaaga gcgcattcat acgattggtt ttttcgattt ccataacttt
    15841 tattatacca aaaattagcc taatctacca cactaggaag ccgatccaag aagatagata
    15901 gctaaatttg aaaaagacat gagcctagcc ccaagtaatt tccaattgat agctggcaaa
    15961 aggatgtccc tcttgatttt gtagttgata atctagttca atcttttgcc tatcaacttg
    16021 ataatggctc gtttggatga taaactcctg catgcccata ggtgtaggaa tataggctaa
    16081 actatcgcta tcctttagaa agcgcataat ggtcttggga ttagaaaatc ggctcatcac
    16141 aagttcttga ccatgaaatt taatcactac tttttccttt tcctcattat agaaaagcag
    16201 gtagctataa tctccttttt catgcacttc cacatcataa agccggtcaa tcacttccaa
    16261 ctgctcatca aactgaattg tatttcgcat ccgaatcttc acatcaggcc ctctttcttg
    16321 tctcttgtcc tactatttta ccaaaaagag caggattttg ctataatggt catatgaacg
    16381 aaaaagtatt ccgtgaccca gttcacaact acatccatgt caataatcaa attatctatg
    16441 acttgattaa tacaaaagaa tttcagcgtt tgcgccgtat caagcaactg ggaacctcca
    16501 gttatacctt ccacggtgga gagcacagcc gcttctctca ctgtctagga gtctatgaaa
    16561 ttgcacgacg catcacagag attttcgaag aaaaatatcc tgaggaatgg aatcctgccg
    16621 agtctctctt gaccatgacc gctgctctcc tacacgacct tgggcatggt gcctactccc
    16681 atacttttga aaatctcttt gatacagacc atgaagccat tactcaggag attattcaaa
    16741 atcctgagac agagattcac caagtcctgc tacaagtggc acctgatttc ccagaaaagg
    16801 tggccagtgt cattgaccat acctatccta ataagcaggt cgtgcagctc atttctagcc
    16861 agattgatgc ggaccgcatg gactatctct tgcgtgactc ctattttaca ggagcatcct
    16921 atggggaatt tgacctgact cgaatcctcc gagtcattcg tcctatcgaa aatggtatcg
    16981 cctttcagcg caatggcatg cacgccatcg aagactacgt cctcagtcgc taccagatgt
    17041 acatgcaggt ttatttccac cccgcaacac gcgccatgga agttctccta cagaatcttc
    17101 tcaaacgcgc caaggaactc tatcctgaag acaaggactt ctttgcacga acttctccac
    17161 acctcctgcc tttctttgaa aaaaatgtga ccttgactga ctatctggct ctagatgatg
    17221 gtgtgatgaa tacctacttt caactctgga tgaccagtcc tgacaagatt ctcgcagact
    17281 tgtcacaacg ctttgtcaac cgcaaggtct ttaaatccat taccttttca caagaggacc
    17341 aagaccaact cgccagcatg agaaaattag ctgaggatat cggctttgat cccgactact
    17401 acactgccat tcataagaac tttgacctcc cttatgatat ctatcgtccc gaatctgaaa
    17461 acccacggac acagattgag attttacaaa aaaatggaga actggccgaa ctctctagcc
    17521 tgtctcttat cgtccaatcc cttgctggca gtcgccacgg agataatcgt ttctatttcc
    17581 ctaaagaaat gttagaccaa aacagcatct tcgcaagcat tacccagcaa tttttacact
    17641 tgattgagaa cgatcatttt accccaaata aaaactagaa gaggaaattt atgagtatta
    17701 aactaattgc cgtcgatatt gacggaaccc tagtcaacag ccaaaaggaa atcactcctg
    17761 aagtcttttc tgccatccaa gatgccaaag aagctggtgt caaagtcgtg attgcaactg
    17821 gccgccccat cgcaggtgtt gccaaacttc tggacgactt gcagttgaga gaccaaggtg
    17881 actatgtggt gaccttcaat ggtgcccttg tccaagaaac tgctactggc catgagatta
    17941 tcagcgaatc cttgacctat gaggattatc tggatatgga attcctcagt cgcaagctcg
    18001 gtgtccacat gcatgccatt accaaggacg gtatctatac tgcaaatcgc aatatcggaa
    18061 aatacactgt acacgaatca accctcgtca gcatgcctat cttctaccgt acccctgaag
    18121 aaatggctgg caaagaaatt gttaaatgta tgtttatcga tgaaccagaa attctcgatg
    18181 ctgcgattga aaaaattcca gcagaatttt acgagcgcta ctccatcaac aaatctgctc
    18241 ctttctacct cgaactcctt acaaagaatg tagacaaggg ttcagccatt actcacttgg
    18301 ctgaaaaact cggattgacc aaagatgaaa ccatggcaat cggtgacgaa gaaaatgacc
    18361 gtgccatgct ggaagtcgtt ggaaaccccg ttgtcatgga aaatggaaat ccagaaatca
    18421 aaaaaatcgc caaatacatc actaaaacaa atgacgaatc cggcgttgcc cacgccatcc
    18481 gaacatgggt actgtaaaag tatcattttt caataagaat tgattagcaa taaaatccaa
    18541 tgaatttttt tagcaaacta tttaatttaa aacaaaataa tcataataga gacacaaatt
    18601 ctgattgtaa caatttttac ctaaacgaat tagaatgtgg ccttactcct gggcaactca
    18661 tactcataga ttggactcaa aaaacaggga gaaattataa tttcccaaga tattttaaat
    18721 actctcttca aattgaccct gaatctacac acaatcaatt atacaaatta ggatacttca
    18781 ctaaaaataa gactttatca tatcttacag tagtagaatt aaaaactata ttatctaaac
    18841 ataatttagc tacttctgga aaaaaagcag aattaattac aagaataatt aataatgtta
    18901 acattgacaa tttagatatt ccgttcgaat ttaaactaac aaaagaagca caaaatctta
    18961 ttatcgaaca tagtgactat atcaaagcat actatgataa agacataact atggaagatt
    19021 attgtaaaga aaaaaacaat atctctttta aagcaacttt tggtgatata aaatggagtc
    19081 tcttaaataa acaagctcat aggaatactg tatcaggaga ttttggatgc ttatctaaca
    19141 cacgaaaggc tcagggaaga catttggaac aagaaggtaa tattaaacat gctttaatat
    19201 attacataga atctttgata attactattt caggattaga aaacaatttt tcagccactg
    19261 attatccagt atattatccc gattcgatac ctgactactc actaaaacat attcaaacat
    19321 taatggaatc attatctgat gacgattatg attttgcttt tgatgaagca ttatttcgct
    19381 tctcaatttt gaatgcaaat cattttttat ctaaggaaga tattgactat ttaagagtta
    19441 atttacctcg ttccac
//
LOCUS       DAVKGU010000030        19195 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKGU010000030 DAVKGU010000000
VERSION     DAVKGU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.030"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(1..633)
                     /locus_tag="VW077_001033"
                     /pseudo
     CDS             complement(1..633)
                     /locus_tag="VW077_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(736..960)
                     /locus_tag="VW077_001034"
                     /pseudo
     CDS             complement(736..960)
                     /locus_tag="VW077_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1025..1321
                     /locus_tag="VW077_001035"
     CDS             1025..1321
                     /locus_tag="VW077_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9455066.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYKDVIDSNGVSIE"
     gene            1442..2386
                     /gene="manA"
                     /locus_tag="VW077_001036"
     CDS             1442..2386
                     /gene="manA"
                     /locus_tag="VW077_001036"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9455067.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(2426..3761)
                     /locus_tag="VW077_001037"
                     /pseudo
     CDS             complement(2426..3761)
                     /locus_tag="VW077_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3989..4738
                     /locus_tag="VW077_001038"
     CDS             3989..4738
                     /locus_tag="VW077_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9455068.1"
                     /translation="MYHIKEAAQLSGISVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            complement(4758..5213)
                     /locus_tag="VW077_001039"
     CDS             complement(4758..5213)
                     /locus_tag="VW077_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9455069.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(5233..6408)
                     /locus_tag="VW077_001040"
     CDS             complement(5233..6408)
                     /locus_tag="VW077_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9455070.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(6467..7312)
                     /locus_tag="VW077_001041"
     CDS             complement(6467..7312)
                     /locus_tag="VW077_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9455071.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            7437..7994
                     /locus_tag="VW077_001042"
     CDS             7437..7994
                     /locus_tag="VW077_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9455072.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            8013..8480
                     /locus_tag="VW077_001043"
     CDS             8013..8480
                     /locus_tag="VW077_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9455073.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            8569..9198
                     /gene="upp"
                     /locus_tag="VW077_001044"
     CDS             8569..9198
                     /gene="upp"
                     /locus_tag="VW077_001044"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9455074.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            9372..9962
                     /gene="clpP"
                     /locus_tag="VW077_001045"
     CDS             9372..9962
                     /gene="clpP"
                     /locus_tag="VW077_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9455075.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            10041..10289
                     /locus_tag="VW077_001046"
     CDS             10041..10289
                     /locus_tag="VW077_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9455076.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            10390..11550
                     /locus_tag="VW077_001047"
     CDS             10390..11550
                     /locus_tag="VW077_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9455077.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11818..12687
                     /locus_tag="VW077_001048"
     CDS             11818..12687
                     /locus_tag="VW077_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9455078.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            12691..13647
                     /locus_tag="VW077_001049"
     CDS             12691..13647
                     /locus_tag="VW077_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9455079.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            13647..14411
                     /locus_tag="VW077_001050"
     CDS             13647..14411
                     /locus_tag="VW077_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455080.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            14411..15121
                     /locus_tag="VW077_001051"
     CDS             14411..15121
                     /locus_tag="VW077_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455081.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGS"
     gene            15535..16191
                     /locus_tag="VW077_001052"
     CDS             15535..16191
                     /locus_tag="VW077_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9455082.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIVRTSAKVL
                     "
     gene            16299..17394
                     /gene="prfB"
                     /locus_tag="VW077_001053"
     CDS             join(16299..16370,16372..17394)
                     /gene="prfB"
                     /locus_tag="VW077_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9455083.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKMSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTYIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSYEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            17412..18104
                     /gene="ftsE"
                     /locus_tag="VW077_001054"
     CDS             17412..18104
                     /gene="ftsE"
                     /locus_tag="VW077_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9455084.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            18097..19023
                     /locus_tag="VW077_001055"
     CDS             18097..19023
                     /locus_tag="VW077_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9455085.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         5895 a         3606 c         4131 g         5563 t
ORIGIN      
        1 ataatcatat ggataagctt gtgaaagctc ttctaaaaag acgttcatcc actcagtatt
       61 acatccacca gctcttaaga aaaatgattc gcctgtatag gcatcaacag ctccataaca
      121 atagcgaaat tctcgtatat agtgactatg aacatgtgga cctactccta tttgagacca
      181 acaagatccc agtttactga ttctaccgaa accagcttca tcttggtaca tcaagcgaac
      241 cttacgaaaa cgtctactgg ttttaaagcg ctttcttgtc ttctttaatt gagattttat
      301 ttttagacac gacaatggtt tgagcgtctg ctttcttagg atgttctgga cgtggcataa
      361 tatttcgcca atcatggcgc ttcaacagtt gatagaaggc atcacgtgtg taggaacgac
      421 ctaactcctt tttataagcc tgaaataagg catcaattgt aacaaattct cctgcctctg
      481 cagccttcaa atggcgggca agaaaggctt tttcttcctc aactgtcata tatgatggtt
      541 acgaccacca cgtgtttctt gaaggagaga gtcgagtccg aactcctcat atttttttac
      601 atttcgccaa atcgttgttt gattacagtc tatagtgtat tgaatctatg acagtacacc
      661 ttggctgcta aaatatttct ataaattaat ttgactttcc tgatagagtt gttcacatct
      721 tatttcaaac cactataaaa gttctataat ctctttataa gatttgccca tcaggcgaaa
      781 tataatagat tgaaactaga atagtacacc tctacttcta aaacattgtt agaaatcgat
      841 ttgtcctgtt cttgtttcat tttactatag aacgatttga aggcgtttat aatatttagc
      901 tgtacgagag tcttttaaaa gtgttttgat ggtttggatt tcttctttag ttgatttcat
      961 attactattg tataatgctt tttgatttta gtttagtatt atcgtagaaa atggactcgg
     1021 tgctatggat acaccggatg agaatggtta tgtagcagat gactatcgga ttacttactt
     1081 agaggcccac atcaaggcca tgcgagatgc catttaccaa gacggggttg acttgcttgg
     1141 ttatacgact tggagctgta tcgatccggt ttcagctggc acaggggaaa tgaataagcg
     1201 ctatggcttt atctatgtag atcgagataa tgtaggaaat gggactctca aacgtagcaa
     1261 gaagaagtcc ttctattggt acaaggatgt cattgatagc aatggtgtaa gcatcgagta
     1321 gagcacattt gtttactatt tttataaaat cttctccctg ctttataaga tgtaaaaaag
     1381 agttcaaatg gaactctttt tttgctataa tgaggggaga aaaatcagac aggagattaa
     1441 gatgtcagaa ccattatttt tacaatcagt tatgcaagaa aaaatctggg gtggtaccaa
     1501 gctacgtgat gagtttggct acgacatccc aagtgaaaaa gtcggagagt actgggctat
     1561 ctcagctcat ctaaatggtg tttctaaagt tgccaatggt cgttacgagg gaacagatct
     1621 tgctactttg tatgcggaac accgtgaatt atttggcaat cgtcctgagc ctgtatttcc
     1681 acttttgacc aagatcctcg atgccaacga ctggctcagt gtccaagttc acccagacga
     1741 tgcttatgga ctcgagcatg aaggcgaact tggaaaaaca gaatgttggt acatcattgc
     1801 agcagatgaa ggttcagaga ttatctacgg tcacaatgcc aagtcaaaag aagaactccg
     1861 ccagcaaatc gaggataaga actgggatgc gctgttgaca aaagtgccag ttaaagcagg
     1921 agatttcttc tatgtaccaa gcggtaccat gcacgctatc ggggcgggta tcttgattct
     1981 tgaaacccag cagtctagcg ataccactta ccgtgtctat gactttgacc gtaaggatga
     2041 caagggcaat ttacgtgaac ttcaccttga aaaatctatc gatgtcttga acattggtga
     2101 acccgcaaat agccgtccag tgacaatcaa agcagatgat ctacgttcca ctctccttgt
     2161 atccaatgat ttcttcgcag tttacaaatg ggaaattact ggaaaagtta actttgaaaa
     2221 gacagctgac tacagcttgt tgagtgtctt ggctggtcaa ggtcaactaa ctgttgacgg
     2281 gaaaaactat ccaatccaaa aagggagaca ctttatccta ccaagtgatg ttgaatcttg
     2341 gactctagaa gggcaagatt tggaattgat tgttagccat ccataaaaag aaagggcctg
     2401 agttcactac tcaagtcctt tttgattaca taaattgggc aatgaagacc acaatgatga
     2461 tgattggaat gacgaaacga agaaacgaag aaggaagagc cagacttgga acagtccttg
     2521 tttccatgct ctttcatcga gatggagttc ctccattgca agagcctttt taaagatata
     2581 gcctgtaaaa agtgaaaggt agagagctcc aaatggcatg aggagattgg aagccaagaa
     2641 gtccatagcg tcaaagaaga tcttaccaaa aatgtgaaca tccgccatga caccgtaaga
     2701 tagggctgaa ggaatgccaa agacaaaggt caaaattcct aaaataacac tccatttggc
     2761 acgtttgctg ttatcctggt tggtgatatt gcccacattg atctccagca tcacgacaga
     2821 agaagtgaca gtcgcaaaga ggaaaaggac gtagaaaatg gttccaaaag gcatcttgtc
     2881 aaaaagttga ggcaagacga taaagagcaa gctcggtccc ccttcagact ggatattgaa
     2941 ggctgacatg gctgggaaaa tggctagacc tgccatgatg gatatcgaga tattcatggc
     3001 tacgatggag attcctgact ggactagatt ggttttcttg tccaagtaag aagcataggt
     3061 caacatgact gtaaccccta gtgagagggc aaagaaagat tgtcccagag catagaggag
     3121 atcagcgcta gtcagttttg aaaagtctgg tttgaggaag taaagaaccc cttccatggc
     3181 atttgacaag ctgagagagt gaccgatgat aacaacaaag atgataaaga gcaggggcat
     3241 catgactttc gaagctcttt caatcccttt ttgaacccca cgtgatacaa tgaagatatt
     3301 caataggata aaggccgctt gagctcctag ggcaatggct ggatttgaaa tgattgaagt
     3361 aaataactga gcataatcac ccgttccacc aagttggaac aatttcccaa actcaatacc
     3421 tagatagact agaatccatc ctccgataac actgtaaaaa gataagagga taaagagggc
     3481 aaaggcgcca atccaaccga taaagttgta cttgttattc ttgcccagtt ttccaaaggt
     3541 tttgatagcg gaaacgccag cactacggcc aagggcaaac tcagccagca ggagagggaa
     3601 atcgattaaa atagtggaaa tgagaaagat tagtaaaaag cctccaccgc cattagcagc
     3661 agtcatgtag ggaaacttcc aaacggcacc aagcccgatg gccagccagc agatgctaga
     3721 ataaaaccaa gtttcgaccc ccattgtgat ttttctgaca tagttaaact ctgatctcct
     3781 ttttcaaaat aagaaaaagc tacttgagtt gtcaaatagc cttctgtttg attgatagac
     3841 ttgactatcc tatcatgttt tctaaggtct gtcaagaaaa tcattttcaa gttttcacac
     3901 ctttctcaaa aaagttaaaa aattttctca aaaacgcttg actctgacct aaggggaggg
     3961 ggtatactat cattgtaagg aggaaatcat gtaccatata aaagaagctg cgcagctttc
     4021 aggtatctct gtcaagaccc tgcatcacta tgacaagata ggactcttgg tccccttaaa
     4081 gtcggaaaac ggctatcgaa cctacagtca agaggatttg gaacgccttc aggtcattct
     4141 ttactacaaa tatctaggct tttctttaga gaaaatagca gagctgttaa aggaagaaag
     4201 gacagattta ttgccccatt tgactaggca gttggactat ctaactcgcg aaaggcaaca
     4261 tctggatacc ttgatttcca ccttgcaaaa aactattcaa gaacaaaaag gagaaagaaa
     4321 aatgaccatt gaggaaaaat tcacgggatt tagctatcaa gacaatcaaa aataccacca
     4381 agaagcggta gagaaatatg gtcaagaagt catgggacaa gcgctcgaac gccaaaaagg
     4441 tcacgaagac gaggctacgg ccgcctttaa ccaagtcttt caaactttgg cacaaaatct
     4501 tcaagttggt ttacctgcaa cagcaaccga aaaccaagag caagcagcca agctcttgca
     4561 agccattcgc acttatggat ttgactgctc tattgaggta ttcggtcata tcggtaaagg
     4621 ttacgtctat aacccagagt ttaaggaaaa cattgacaag tttggttctg gaacagccca
     4681 gtacacgtca gatgccattg cagcttacgt tcagacaaat gcagaaaaaa taggctagga
     4741 atttcctagc ctatttttta cttcaaatca taaagccagt cgtcaccgtt tttgtagtaa
     4801 aagaattcac tgagatcttc ttctagaaac acacgaagca tatcagacat atcatcggtt
     4861 gcaagtttta gatgagaaag attttcaaag tcctcccacc aaactttccc ttcgtctgaa
     4921 gactggagtt caccagtaaa gtgttctgtc ttgtaaaaaa ggacgacata acgataatcc
     4981 ttgtcgtcat accagttttt gataccacag agttggggtt tggaaatgat cagaccagtt
     5041 tcttctttca cttcacgaat gacagcatcg acaaaggatt cgccacgttc aacatgacca
     5101 ccaggaaaag taatgccagg ccagtcggga ctaactcggt cttggaccag gaccttatct
     5161 ccgtttttaa tcatacacat gttaacaaat tcgactgcct ctcttctgtt cattcttcac
     5221 aacctttaat ctttaatcat aatgcagact tcccgccacc cagccggtac agagggcaga
     5281 agtgatgtta aagccacccg tgtgggcatt gatatccata acttcgcctg caaagtggag
     5341 gccaggtacc agcttacttt caagggtttt aggattgatt tccttgagac tgactccacc
     5401 cttggtaaca aaggactttg caagggacat ttttccagtt acaggaattt taagttcttt
     5461 aatggactgg acaagctgtt ctcgttcctt ttcagtcagc tgtttgactt tttcaggata
     5521 tccttgtaca aaaaattcgg ccaagcgttc tggtaacaag gtttttaaag cgtttttcaa
     5581 ggatttttcc cgattttctt ctagaaatgt aaccaagtcc ttctcagaaa gttgaggcaa
     5641 aacatcgagt gagagaacct ccccaccttt gacaaagcta gacatgcgta gggcagcagg
     5701 acctgacaaa ccaaagtggg taaagagtaa atcatgagtg atgacatgct taccataact
     5761 tagggtcaca tcgtccagag aaatcccttg taaggcttta tgtggaaaat ctgttaataa
     5821 aggactttca gcagcctcaa gatcggtgat ggtatgctta aaatggcgag caatctcgtg
     5881 accaaaacca gtcgaaccag tcgaaggata agacttacca cctgttgtga caatgagttt
     5941 ctcacaagtg aaggtttgat ccgctgactt aaggacaaac tggtcatcta cttttttaac
     6001 agaaacgatt tctatttgag tagcaacttg accacctagt tcagtgattt tcttttccaa
     6061 agcttcgata atagtccgag acttgtcact ggctggaaag acgcgtccgt ggtcttcgac
     6121 cttaagttta acaccatttt ctgtaaaaaa gttgatgatg tcatgattat cgaactggga
     6181 gaaaacactg taaagaaagc gtccgtttcc aggaattcca gctagcaggt tgtctaagct
     6241 accattgttg gtcacattgc aacgtccccc accagtccca gctaattttt ttccaagttt
     6301 ccgatttttt tcgatgagga gggttttctg tccataaaag ctactggaaa tcgtagccat
     6361 cataccagca ggtcccccac cgatgacaat agtatcaaaa tgtttcatag ctctattgta
     6421 ccacaaaaaa acaagagatg atggtcacct cttgtcaaga atgcaattaa tcaatttcat
     6481 agcccatcag caaaccgccc tcttctgcat agaaactgca gagaccagag gttggtagaa
     6541 ttttaatatc cgcttgtggg aaggtttcac ggattcgctc tgagagctgt tgacaacatt
     6601 tttcgttatt gcgttgggcc atgacaatac ggccaccagc atatccagct tttactaact
     6661 catcataggc agcttgaact gatttctttg atccccttgc tttttgtagc aattcgagag
     6721 ttccagtttc actagctttt ccgaccatac gaatgttgag aaggccaacg accgtaccga
     6781 taagcttgct caaacggccg ttcttcacca agttatcgac tttggctagg acaaagagca
     6841 acttagtttt ttcttgatag gcggtgatag cttcaaccac ttcttcaaaa gacaagccct
     6901 ggtcaatcaa gtcattcaat ttttctacga gtaggtcaac ttcaccacca gcagataaac
     6961 tatcaatcac atgaatctta gtgtcaggat ggtcttccag ataaatattc tttgctagtt
     7021 gagcactatt gtgactgcca gaaagggtac ctgtgatggt tactaggaaa atgtttttgg
     7081 caccttcaaa tgctcgcaaa tagtcatctg ggcttggaca agccgatttt gaagcttctg
     7141 cagttgcata catggtttcc atcatttggt caatatcgag actggcgtca tcaacaaaga
     7201 cctgatcagc tacttgaatg gttaagggga cacttacaaa ggttgtgtta atagctggtg
     7261 ttggcagttg acgataatca caaccagagt cagcaataat tttccaagtc atagaaattc
     7321 tccatctttg tcagttatac attgacaaag gttctgtctt tttttacaat tatatcatga
     7381 aaactcttga aacaaaagca tcatccgtct gttgtgacaa gtagaaagaa aatgttatgt
     7441 ctgaacgtag aatctctgaa aagtctcttg aaaatctcag aaaatcaaac caagaatcca
     7501 atttattaac cagagaagcc attgaaacag ccctcttgca actcttggaa aaaaaggagc
     7561 tgaccaagat tagtatttct gaattggtca aacgtgcagg tgtttcgcgt gcggcctttt
     7621 atcgcaatta tgactccaaa gaggaaattt tagagagcgt ctttaaacga actgtccaca
     7681 atattatgga acagctgcat cattacgatt taaagacaga cctttatttg gtttgggttc
     7741 accttttccg ggaggccaga aaggaagcta gagtgattca attggccttg gattaccatc
     7801 tggaaaaaat ctttgtccaa gccatgcagg aatttctaga aaaataccat gggaaatcaa
     7861 aaggtgtcag ctcttatctt cattccttct ggagctcagc cattgtctct gtccttctaa
     7921 aatggatcaa ggatggcatg aaggtaccag ctgaaaagat tgcagattta ggtttaccat
     7981 tttttaaaaa atagagaaaa aggagaaaag agatgactga aaaaagactg gcctgggatg
     8041 agtattttgc agcccaagcc ctactaattg cgaatcgttc cacttgtaaa cgtgctaaag
     8101 tgggcgcgat tctggtaaaa gataataagg ttatttccac tggttacaat ggttcggtgt
     8161 cagggactga gcattgtatt gatcacgaat gtttggtcat tgaaggccac tgtgttcgca
     8221 cccttcacgc tgaggtcaat gctatccttc aaggtgctga gcgtggtgtt cccaaaggct
     8281 ttacagccta tgtaacccat tttccttgtc tcaactgtac aaaacaattg cttcaggtcg
     8341 gctgcaagcg tgtggtttat atcaaccagt accgaatgga cgactacgcc caataccttt
     8401 atcaagaaaa agggacagaa ttgacccatt taccacttga gacagtacag acagctctta
     8461 aagaggctga tctaatgtaa aaattatcaa aaataaatgg tttagaaaga tttttaaacc
     8521 gttttttggt ataataaaaa gaataaattg aaagaaggaa ttccaaaaat gggaaaaatt
     8581 gaagttatta atcacccact gattcaacac aaattgtcaa tcttgcgtcg tacagatact
     8641 tctacaaaag cttttcgtga gctagtagat gagattgcaa tgttgatggg gtatgaagta
     8701 cttcgtgatc ttccactaga agatgtggaa atcgaaacac caattacaaa aacagttcaa
     8761 aaacaattgg caggtaagaa attggccatc gtcccaatct tgcgtgcagg tatcgggatg
     8821 gttgatggtc tcttgagctt ggttccagct gctaaagttg gccacatcgg tatgtaccgt
     8881 gatgaagaaa cacttcaacc agttgaatac ttggtgaaat tgcctgagga cattgaccaa
     8941 cgtcaaattt ttgtagtaga cccaatgttg gcaacaggtg gctcagcaat cttggctgtt
     9001 gattctctta aaaaacgtgg cgcatcaaat atcaaatttg tctgccttgt atctgctcca
     9061 gagggtgtaa aagcccttca agaagctcat ccagatgtag aaatctttac agcagccttg
     9121 gatgaacgtt tgaatgaaca cggttatatc gttccaggtc ttggagatgc tggagaccgc
     9181 ttgttcggta caaaataaga tcgaaaagaa gaatgacttg gaaaagtatt tctagtcacg
     9241 aaaggaggtt gggtttttgt ttctgtctaa tgaaagcaga gcaaaaattt gacctttttt
     9301 gaccaagata ttataatagt cttatcttca gtcatttgac caacaaaatt aaaactcaaa
     9361 aggagaaatg aatgattcct gtagttattg aacaaacaag ccgtggagaa cgttcttacg
     9421 atatttactc acgtcttctc aaagaccgca tcattatgct gacaggtccg gttgaagaca
     9481 atatggctaa ctctgttatt gcccaattgc ttttcttgga tgcccaagat agtacgaaag
     9541 atatttacct ttatgtcaat acaccaggtg gttctgtttc agctggtttg gcaatcgtag
     9601 ataccatgaa ctttatcaag gcagatgtcc aaaccattgt tatgggaatg gctgcatcta
     9661 tggggactgt catcgcatca agtggagcaa aaggcaaacg tttcatgctt ccaaatgctg
     9721 aatacatgat tcaccaacca atgggcggta caggtggtgg tacccaacaa actgatatgg
     9781 ctatcgctgc agaacacttg ctcaaaactc gtaatacctt ggaaaaaatc ttggctgaaa
     9841 attcaggtca gtcaatggaa aaagtccatg cagatgcaga acgtgataac tggatgagcg
     9901 cccaggaaac acttgaatat ggctttattg atgaaattat ggccaacaat tcattgaact
     9961 aatgatgata gaaggcaaac tcgactgggt ttgctttttt tggtataata gggagagatt
    10021 tcttagaaag aggatttatc atgtttgaaa aagtcaatcg ctctggcttg attatctatc
    10081 tttactataa tcgtgatgcc aaaaaactgc aggattatgg agatattacc tatcattcca
    10141 agaaacatcg ttacttacaa ctctatgttc caactcaaga agtggagcaa ttggtcggac
    10201 gcttgagcaa ggaaaaattt attaaaaaag ttagggtttg tcatatccaa gagttggaaa
    10261 caccctttgt gggcaatctt tatcgatagg aaaacgttat catcgaaaaa gttcaaggaa
    10321 aacattgaca attttctgat aattcggtat atttttaaca gactgtttaa gaaataagga
    10381 gacaaaaaga tgaagaaaaa atttgcccta tcgtttgtgg cgcttgcaag tgtagcactt
    10441 cttgcagcct gtggagaagt gaagtctgga gcagtcaaca ctgctggtaa ctcagtagag
    10501 gaaaagacaa ttaaaatcgg gtttaacttt gaagaatcag gttctttagc tgcatacgga
    10561 acagctgaac aaaaaggtgc ccaattggct gttgatgaaa tcaatgccgc aggtggtatc
    10621 gatggaaaac aaatcgaagt agtcgataaa gataataagt ctgaaacagc tgaggctgct
    10681 tcagttacaa ctaaccttgt aacccaatct aaagtatcag cagtcgtagg acctgcgaca
    10741 tctggtgcga ctgcagctgc ggtagcgaac gctacaaaag caggtgttcc attgatctca
    10801 ccaagtgcga ctcaagatgg attgactaaa ggtcaagatt acctctttat tggaactttc
    10861 caagatagct tccaaggaaa aattatctca aactatgttt ctgaaaaatt aaatgctaag
    10921 aaagttgttc tttacactga caatgccagt gactatgcta aagggattgc caaatctttc
    10981 cgcgagtcat acaagggtga aatcgttgca gatgaaactt tcgtagcagg tgacacagac
    11041 tttcaagcag cccttacaaa aatgaaaggg aaagactttg atgctatcgt tgttcctggt
    11101 tactataatg aggctggtaa gattgtaaac caagcgcgtg gcatgggaat tgacaaacca
    11161 atcgttggtg gtgatggatt caacggtgag gagtttgtac aacaagcaac tgctgaaaaa
    11221 gcatcaaaca tctactttat ctcaggcttc tcaactactg tagaagtttc agctaaagct
    11281 aaagccttcc ttgacgctta ccgtgctaag tacaatgaag agccttcaac atttgcagcc
    11341 ttggcttatg attcagttca ccttgtagca aacgcagcaa aaggtgctaa aaattcaggt
    11401 gaaatcaagg ataaccttgc taaaacaaaa gattttgaag gtgtaactgg tcaaacaagc
    11461 ttcgatgcag accacaacac agtcaaaact gcttacatga tgaccatgaa caatggtaaa
    11521 gttgaagcag cagaagttgt aaaaccataa tagaaaaatg ttgaaatagg gaatgagcct
    11581 ttgactcact ccctgtttcg atatttaata ctcttcgaaa atctcttcaa actgcgtcaa
    11641 cgtcgccttg gattatatat gtgactgact tcgtcagtct tatctacaac ctcaaagcag
    11701 tgctttgagc aacctgcggc tagtttccta gtttgctctt tgattttcat tgagtataag
    11761 aacctatcaa aaagtgaggg aaacccctcg gaattataaa tagaaagagt gaatcttatg
    11821 ctccaacaac tcgtaaatgg tttgattcta ggtagtgttt acgcgctgtt agccctagga
    11881 tataccatgg tttacggaat tatcaagctc atcaacttcg cccatggtga tatttatatg
    11941 atgggagcct ttatcggtta tttcttgatc aattctttcc aaatgaattt ctttgtagcg
    12001 cttattgtag ctatgctagc gacagctatt cttggtgtcg tgattgagtt tcttgcttac
    12061 cgacctttgc gccactctac tcgtattgct gttttgatta cggctattgg ggtttctttc
    12121 ctattggagt atggaatggt ctatctggtt ggtgccaata cccgtgcctt ccctcaagcg
    12181 attcaaacag ttcgatatga tttgggacca attagcttaa caaatgtgca gttaatgatt
    12241 ttggccattt ccttgatttt gatgattttg ttacaagtca ttgtccaaaa gactaagatg
    12301 gggaaagcca tgcgtgcagt atcagtagat agcgacgcgg cgcaattgat ggggatcaat
    12361 gtaaaccgta cgattagctt taccttcgct ttgggttctg ctcttgcggg tgcggctggt
    12421 gttctgattg ctctttatta taactctctt gagcctttga tgggggttac tccaggtctt
    12481 aaatctttcg ttgccgcagt acttggtggt atcggaatta ttcctggtgc tgctcttggt
    12541 ggctttgtga ttggtctatt ggaaaccttt gcgacagcct ttgggatgtc agatttccgt
    12601 gatgccattg tttatggaat cttgttgttg atcttgattg tccgcccagc tggtatcctt
    12661 ggtaagaatg tgaaagagaa ggtgtaaacg atgaaggaaa atttaaaagt taatattcta
    12721 tggttactcc ttttgttagc tggctatagc ttgattagtg tactggtttc agtcggagta
    12781 cttaatctat tctatgtaca gattttacaa caaattggaa ttaatattat tttggctgtt
    12841 ggtctcaact taatcgttgg tttttcagga caattttcac ttggtcatgc tggtttcatg
    12901 gcgattggtg cctatgcagc agctattatt ggttctaaat caccaaccta cggtgccttc
    12961 tttggagcta tgcttgtagg ggctttgctt tcaggagcag ttgccttact tgtcggcatt
    13021 ccaaccttgc gcttgaaggg ggactatctt gcggtagcaa ctctgggtgt ttctgaaatt
    13081 atccgtatct ttatcatcaa tggtggaagt cttacaaatg gtgcggcagg tatcttaggg
    13141 attcctaact ttacaacttg gcaaatggtt tacttctttg tcgtgattac aaccattgca
    13201 accttgaact tcttgcgtag cccaattggc cgttcaaccc tctctgttcg tgaagatgaa
    13261 atcgctgctg agtcagttgg ggttaatacg actaaaatta aaatcatcgc ttttgtcttt
    13321 ggtgccatta ctgcaagtat tgctgggtca cttcaggcag gatttatcgg gtctgttgta
    13381 ccgaaagatt acaccttcat caactcaatc aacgttttga ttattgttgt atttggtgga
    13441 ctcggttcca ttacaggtgc gattgtttcg gctattgttc tgggaatttt gaatatgctt
    13501 ctccaagatg ttgctagtgt gcgtatgatt atttacgctt tggccttggt attggtaatg
    13561 attttcagac caggtggact ccttggaacg tgggaactga gcctatcacg tttctttaaa
    13621 aaatctaaga aggaggaaca aaactaatgg cattacttga agtaaaacag ttaaccaaac
    13681 attttggtgg tctaacagct gttggagatg tgactcttga attgaacgaa ggggaactgg
    13741 ttggattaat cggtccaaac ggagctggga aaaccaccct tttcaacctt ttgaccggtg
    13801 tttatgaacc aagcgaggga acagtaaccc tagatggtca ccttttgaat gggaaatcac
    13861 cttataagat tgcctctttg ggacttggac gtactttcca aaatatccgt ctctttaaag
    13921 atttaacagt tttagacaat gttttgattg cttttggaaa ccatcacaaa caacatgttt
    13981 ttactagttt cttacgctta ccagcttttt acaagagtga aaaagaatta aaggctaaag
    14041 ctttggaatt gttgaaaatc tttgatttag atggtgatgc agagactctt gctaaaaatc
    14101 tttcctacgg acaacaacgt cgtttggaaa ttgttcgtgc ccttgctacg gaacctaaaa
    14161 ttctcttctt agatgaacca gcagcaggta tgaacccaca ggaaacagcc gaattgactg
    14221 agttaattcg tcgtatcaaa gatgaattta agattacaat catgttgatt gaacacgata
    14281 tgaatctggt catggaagta acagaacgta tctacgtact tgaatatggc cgtttaattg
    14341 ctcaaggaac tccagacgaa attaagacca ataaacgcgt tatcgaagct tatctaggag
    14401 gtgaagccta atgtctatgt taaaagttga aaatctttct gtgcattacg gtatgatcca
    14461 agcagtccgt gatgtaagct ttgaagttaa tgaaggagaa gttgtttccc ttatcggtgc
    14521 caacggtgca ggtaagacaa ctattcttcg caccttgtca ggtttggttc gaccaagttc
    14581 aggaaagatt gaatttttag gtcaagaaat ccaaaaaatg ccagctcaga aaattgtggc
    14641 aggtggtctt tcacaagttc cagaaggacg ccacgtcttt cctggcttga ctgttatgga
    14701 aaatcttgaa atgggagctt tcttaaagaa aaatcgtgaa gaaaatcaag ctaacttgaa
    14761 gaaggttttc tcacgctttc ctcgtcttga agaacgtaag aaccaagatg cagctactct
    14821 ttcaggaggg gaacaacaaa tgcttgccat gggacgcgct cttatgtcaa caccaaaact
    14881 tcttctttta gatgaaccat caatgggact tgccccaatc ttcatccaag agatttttga
    14941 tatcattcaa gatattcaga agcaaggaac aaccgtcctc ttgattgaac aaaatgccaa
    15001 taaagcactt gcaatctctg accgaggata tgtactggaa acagggaaaa tcgtcctatc
    15061 aggaacagga aaagaactcg cctcatcaga agaagtcaga aaagcatatc taggtagcta
    15121 aaacaatcca gtggattgtt ttagtcggca gatgaagatt acgaagtaat catcattata
    15181 gtctgggaga ctagtttagg ttgcagataa agattgcgaa gcaatcatca aatccagagg
    15241 attgttttag ccggcagatg gagattacga agtaatcatc aatatagtcc tggggacctt
    15301 tttagtcggt ggatagagat tgcaaacaaa tatgcatcta cattgaaagc ttaatttcta
    15361 ataattgaaa aaatcgaatg aaaaatttct taccttcatt cacagagctc tatttcagag
    15421 ctctttttgc tagcttattc atactttttt gaatttcgaa aaagaaatgt aagcgtttga
    15481 tagatttaca aaaagattgt ataatgggga taagaataga aaaggagaag tctcatggca
    15541 gttaaagatt ttatgacccg caaggtagtt tatattagtc cagatacaac agtatctcat
    15601 gcagcagatt tgatgagaga gcaagaattg catcgcttgc ctgttatcga aaatgatcaa
    15661 ttggttggtt tggtgactga gggaaccatt gcacaagcaa gtccatctaa agcaacaagt
    15721 ctttctatct atgagatgaa ttatcttctg aataagacaa aagtaaaaga tgtcatgatt
    15781 cgcgatgttg tcactgtctc aggctatgct agtctagaag atgcaactta tctgatgttg
    15841 aaaaataaga ttggtattct ccctgtcgta gataaccatc aagtatacgg agttattact
    15901 gaccgtgacg ttttccaagc ctttcttgaa attgcaggtt atggcgaaga agggattcgt
    15961 gtacgctttg ttacagaaga tgaagttggt gttcttggaa aaattgtttc tttgattgta
    16021 gaagaaaatt tgaatatctc ccatacagtc aatattccgc gtaaggatgg taaggtgatt
    16081 atcgaagtgc aaatcgatgg atcaattgat ttaccagcct tgaaagaaaa atttgaagca
    16141 aatggtattc aagtggaaga aatcgttcgc acttcagcaa aagtcttgta agaagggaag
    16201 cccaaaggct tcttttttca tgaaaagggg attagagcaa aagatggaaa gaaatgataa
    16261 aatatgctat aatgaaataa tgtaaaaaag gagtatttat ggacatttca gtaattcgtc
    16321 agaaaattga cgcaaatcgt gaaaaattag cttctttcag ggggtctctt tgacctcgaa
    16381 gggctagagg aagagattgc catcttggaa aacaagatga cagaacctga tttttggaac
    16441 gataatattg cggcccaaaa aatgtcgcaa gaattaaatg aattaaaaaa cacttacaat
    16501 accttccata agatggaaga gttgcaggat gaagtcgaaa ttttattgga ttttttggct
    16561 gaagacgagt cagtgcatga tgaactggta gcgcagttag ccgaacttga taagataatg
    16621 accagctacg agatgactct actcttgtca gaaccttatg accacaacaa tgccatcttg
    16681 gaaatccatc caggttctgg tggtactgag gcgcaggact ggggtgatat gttgcttcgt
    16741 atgtatactc gttatggtaa tgctaaaggc tttaaagtgg aagtgttgga ttaccaagca
    16801 ggtgatgagg ctggtattaa gtcggtaact ttatcatttg aagggcctaa tgcctatggt
    16861 ctcctcaagt cagaaatggg tgttcaccgc ttagtgcgaa tctcaccatt tgactctgcc
    16921 aaacgtcgcc atacctcttt cacatctgta gaagtgatgc cagaattgga tgatactatt
    16981 gaagtggaaa tccgtgaaga tgatatcaag atggatacct tccgttcagg tggtgccggt
    17041 ggacaaaacg tcaataaggt ttcaacaggt gtacgtttaa cctacattcc aactggaatt
    17101 gttgtccaat caacagtgga tcgtacccag tatggaaata gagatcgcgc catgaagatg
    17161 ttgcaggcta agctctatca aatggagcaa gagaagaagg ctgcggaggt agattctctc
    17221 aaaggtgaga aaaaggagat cacttgggga agtcaaatcc gttcttatgt cttcacgcct
    17281 tatactatgg taaaagatca ccgaactagc tatgaggttg ctcaggtaga taaggttatg
    17341 gatggagacc tagatggttt tatcgatgct tatctcaagt ggcgaattag ctaagataga
    17401 aaggaactca catgtcaatt attgaaatga gagatgtcgt taaaaaatac gacaacggaa
    17461 cgactgctct acgcggtgtt tcggttagcg ttcaaccggg ggaatttgct tacatcgtag
    17521 gaccttcagg agcagggaag tcaactttta ttcgttctct gtatcgtgaa gtaaaaatcg
    17581 ataaaggaag tctatcagtt gctggtttta atctggttaa gatcaaaaag aaagatgtcc
    17641 cgcttctacg tcgtagtgtt ggggttgtct tccaagatta taaattgtta ccaaagaaaa
    17701 ctgtctatga aaatattgct tacgctatgg aagtaatcgg ggaaaatcgc cgtaatatca
    17761 aaagacgagt gatggaagtt ttggacttgg ttggattgaa gcataaggtt cgttctttcc
    17821 caaatgaact ctcaggtggg gagcaacagc ggattgcgat tgcgcgtgca attgtaaata
    17881 atcccaaagt attgatagct gatgagccaa caggaaatct ggatccggat aattcatggg
    17941 aaattatgaa tctcttggaa cggattaacc tacaaggaac aactattttg atggcgactc
    18001 ataatagcca gattgtaaat accttgcgcc accgtgtcat tgccattgaa aatggccgtg
    18061 tcgttcgtga cgaatcaaaa ggagagtatg gatacgatga ttagtagatt ttttcgccat
    18121 ttatttgaag ccttaaaaag tttgaaacga aatggttgga tgacagtagc tgctgtcagt
    18181 tcagtcatga ttactttgac cttggtggca atatttgcat ctgttatttt caatacagcg
    18241 aaactagcta cagatattga aaataatgtc cgtgtagtag tttatatccg aaaggatgtg
    18301 gaagataata gtcagacaat tgaaaaagaa ggtcaaactg ttacaaataa tgactaccac
    18361 aaggtatatg attctttgaa gaacatgtct acggttaaaa gtgttacctt ttcaagtaaa
    18421 gaagaacaat atgaaaaatt aaccgagata atgggagata actggaaaat ctttgaagga
    18481 gatgccaatc ctctctatga tgcctatatt gtagaggcaa acactccaaa tgatgtaaaa
    18541 actatagccg aagatgctaa aaaaattgaa ggtgtctctg aggttcaaga tggcggtgcc
    18601 aatacagaaa gactcttcaa gttagcttca tttatccgtg tttggggact agggattgct
    18661 gctttgttaa tttttatcgc agttttcttg atttcaaata ccattcgtat taccattatt
    18721 tcccgcagtc gcgaaattca aatcatgcgc ttggtcggag ctaaaaacag ttatatccgt
    18781 ggaccgttct tgttagaagg agcctttatc ggtttattgg gagctatcgc accatctgtt
    18841 ttggtcttta ttgtttatca aattgtttac caatctgtca acaaatcgtt ggtagggcaa
    18901 aatctatcca tgattagtcc agatttattt agtccgttga tgattgccct actatttgtg
    18961 attggggttt tcattggttc attgggatca ggaatatcca tgcgccgatt cttgaagatt
    19021 taggtaaaat agctgctttt atgaggagat tgtaaaatct ccttttttgc tacaagagtt
    19081 tttgaaaaga gatgcgtaga agaaaagagc ttccaaagaa gtcctctaga gaagacttct
    19141 tgatggctaa tttgaacttg aaatttgact cactagaaat tttttagaaa aaagt
//
LOCUS       DAVKGU010000031        18815 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKGU010000031 DAVKGU010000000
VERSION     DAVKGU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.031"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            176..913
                     /locus_tag="VW077_001056"
     CDS             176..913
                     /locus_tag="VW077_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9455086.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            910..2601
                     /locus_tag="VW077_001057"
     CDS             910..2601
                     /locus_tag="VW077_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9455087.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDARGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            2717..3280
                     /locus_tag="VW077_001058"
     CDS             2717..3280
                     /locus_tag="VW077_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9455088.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            3302..8791
                     /locus_tag="VW077_001059"
     CDS             3302..8791
                     /locus_tag="VW077_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU9455089.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKSELQEQLRVAEQKQQADYSAKTWREFKIAELQAKEINNPTTPLPKQSEIDAAT
                     KALQDALQALAVDKTVLQNAINTANSKREEEYTAQTWKALKDALTAVNPVNEDETATQ
                     SKVDEATRNLEEAINNLVLLTEKPVLTFIETDKKALEREVVAKYSLENQNKTKIKSIT
                     ATLKKGETVVSTVELIGDDVTNETITSAFKNLEYYKEYTLSTTMVYDRGDGDVTEILD
                     NQPIQLDLKKVELKNIKRTDLIKYENGKETNESLITTVPDDKRNYYLKITSKNQKTTL
                     LAVKNIEETTVNGTPVYKVTAIADNLVSRTADNKFEEEYVHYIEKPKVHEDNVYYNFK
                     ELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYITKEFTGKLLSSEGKQFAITELEHPL
                     FNVITNATINNVNFENVEIERSGQDNIASLANTMKGSSVITNVKITGTLSGRNNVAGF
                     VNNMNDGTRIENVAFFGKLHSTSGNGSHTGGIAGTNYRGIVRKAYVDATITGNKTRAS
                     LLVPKVDYGLTLDHLIGTKALLTESVVKGKIDVSNPVEVGAIASKTWPVGTVSNSVSY
                     AKIIRGEELFGSNDVDDSDYASAHIKDLYAVEGYSSGNRSFRKSKTFTKLTKEQADAK
                     VTTFNITADKLESDLSPLAKLNEEKAYSSIQDYNAEYNQAYKNLEKLIPFYNKDYIVY
                     QGNKLNKEHHLNTKEVLSVTAMNNNEFITNLDEANKIIVHYADGTKDYFNLSSSSEGL
                     SNVKEYTITDLGIKYTPNIVQKDNTTLVNDIKSILESVELQSQTMYQHLNRLGDYRVN
                     AIKDLYLEESFTDVKENLTNLITKLVQNEEHQLNDSPAARQMIRDKVEKNKAALLLGL
                     TYLNRYYGVKFGDVNIKELMLFKPDFYGEKVSVLDRLIEIGSKENNIKGSRTFDAFGQ
                     VLAKYTKSGNLDAFLNYNRQLFTNIDNMNDWFIDATEDHVYIAERASEVEEIKNSKHR
                     AFDNLKRSHLRNTILPLLNIDKAHLYLISNYNAIAFGSAERLGKKSLEDIKDIVNKAA
                     DGYRNYYDFWYRLASDNVKQRLLRDAVIPIWEGYNAPGGWVEKYGRYNTDKVYTPLRE
                     FFGPMDKYYNYNGTGAYAAIYPNSDDIRTDVKYVHLEMVGEYGISVYTHETTHVNDRA
                     IYLGGFGHREGTDAEAYAQGMLQTPVTGSGFDEFGSLGINMVFKRKNDGNQWYITDPK
                     TLKTREDINRYMKGYNDTLTLLDEIEAESVISQQNKDLNSAWFKKIDREYRDNNKLNQ
                     WDKIRNLSQEEKNELNIQSVNDLVDQQLMTNRNPGNGIYKPEAISYNDQSPYVGVRMM
                     TGIYGGNTSKGAPGAVSFKHNAFRLWGYYGYENGFLGYASNKYKQQSKTDGESVLSDE
                     YIIKKISNNTFNTIEEFKKAYFKEVKDKATKGLTTFEVNGSSVSSYDDLLTLFKEAVK
                     KDAETLKQEANGNKTVSMNNTVKLKEAVYKKLLQQTDSFKTSIFK"
     gene            8852..10573
                     /gene="pabB"
                     /locus_tag="VW077_001060"
     CDS             8852..10573
                     /gene="pabB"
                     /locus_tag="VW077_001060"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9455090.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADFLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELILEEN"
     gene            10574..11191
                     /locus_tag="VW077_001061"
     CDS             10574..11191
                     /locus_tag="VW077_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9455091.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGIKDKPNL
                     SSMRSLHKLISESQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            11241..11999
                     /locus_tag="VW077_001062"
     CDS             11241..11999
                     /locus_tag="VW077_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9455092.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLTSDGSYAYSTWQGN
                     YYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKSYNS
                     LFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            12126..13085
                     /locus_tag="VW077_001063"
     CDS             12126..13085
                     /locus_tag="VW077_001063"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9455093.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGNNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            13182..14021
                     /locus_tag="VW077_001064"
     CDS             13182..14021
                     /locus_tag="VW077_001064"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9455094.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(14069..14239)
                     /locus_tag="VW077_001065"
     CDS             complement(14069..14239)
                     /locus_tag="VW077_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9455095.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            14360..15244
                     /gene="miaA"
                     /locus_tag="VW077_001066"
     CDS             14360..15244
                     /gene="miaA"
                     /locus_tag="VW077_001066"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9455096.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            15237..16475
                     /gene="hflX"
                     /locus_tag="VW077_001067"
     CDS             15237..16475
                     /gene="hflX"
                     /locus_tag="VW077_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9455097.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     NYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDS
                     EVITGYISEKNKWRLEEFYD"
     gene            16468..17091
                     /locus_tag="VW077_001068"
     CDS             16468..17091
                     /locus_tag="VW077_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9455098.1"
                     /translation="MTDIKALALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     DSDGLGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            17106..18035
                     /locus_tag="VW077_001069"
     CDS             17106..18035
                     /locus_tag="VW077_001069"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9455099.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKISKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            18056..18811
                     /locus_tag="VW077_001070"
     CDS             18056..18811
                     /locus_tag="VW077_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9455100.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLVGETFNYK"
BASE COUNT         6002 a         3435 c         4104 g         5274 t
ORIGIN      
        1 gtactttgag ctgacttcgt cagttttatc tacaacctca aagcggtact ttgagaaacc
       61 tgtggctatc ttcctagttt actttttgat ttttattgag tataagaggt ctttcctaag
      121 cagttagagg agaattttgc tatactgata ctagtaagtg gtaaaggagc agagcatgac
      181 ctacacaatc ttaatcgtag aagatgaata tctggtaaga caaggtttga ctaaactggt
      241 caatgtagca gcctacgata tggaaatcat cggtcaggct gaaaatggaa ggcaggcttg
      301 ggaattgatc caaaagcagg tgccagatat cattttaacc gatatcaaca tgcctcatct
      361 aaatggcatc cagttggcca gtctggtacg agaaacctat cctcaggttc atttggtctt
      421 tttaacaggt tacgatgatt ttgattatgc cttgtctgct gtcaaactag gtgtggacga
      481 ctacctgctc aaaccctttt ctcgtcagga tattgaggaa atgttgggga aaatcaaaca
      541 aaaactagac aaggaagaga aagaagagca gttacaagat ttattgacta ataggtttga
      601 aggaaacatg gcccagaaaa tccagtctca tctggctgat agccaattta gtttaaagtc
      661 tttagccagt gacttaggtt ttagtccgac ctatctgagt tccttgatta agaaagagtt
      721 gggcttgcct tttcaggatt atctggtgag agaacgtgtt aaacaagcca agctcttgct
      781 tttaactaca gatctgaaga tttatgagat cgcagagaag gttggttttg aagatatgaa
      841 ctattttacc caacgtttta agcagattgc aggtgtgaca cctcgtcagt ttaagaaggg
      901 agaagaccga tgaagcgttc ttctctttta gttagaatgg ttatttccat ctttctggtc
      961 tttctcattc tcctagctct ggttggaact ttctactatc aatcaagttc ttcagccatt
     1021 gaggccacca ttgagggcaa cagccaaacg accatcagcc agactagcca ctttattcag
     1081 tcttatatca aaaaactaga aaccacctcg actggtttga cccagcagac ggatgttctg
     1141 gcctatgctg agaatcccag tcaagacaag gtcgagggaa tccgagattt gtttttgacc
     1201 atcttgaagt cagataagga cttgaaaact gttgtgctgg tgaccaaatc tggtcaggtc
     1261 atttctacag atgacagtgt gcagatgaaa acttcctctg atatgatggc tgaggattgg
     1321 taccaaaagg ctattcatca gggagctatg cctgttttga ctccagctcg taaatcagat
     1381 agtcagtggg tcatttctgt cactcaagaa cttgttgatg caaggggagc caatcttggt
     1441 gtgcttcgtt tggatatttc ttatgaaact ctggaagcct atctcaatca actccagttg
     1501 gggcagcagg gctttgcctt cattatcaat gaaaaccatg aatttgtcta ccatcctcaa
     1561 cacacagttt atagttcgtc tagcaaaatg gaggctatga aaccctacat cgagacaggt
     1621 cagggttata ctcctggtca caaatcctac gtcagtcaag agaagattgc aggaactgat
     1681 tggacggtac ttggcgtgtc atcattggaa aagttagacc aggttcggag tcagctcttg
     1741 tggaccttgc ttggggccag tgtcacatct cttcttgttt gtctctgctt agtgtggttc
     1801 agtcttaaac gctggattgc tcctttgaag gatttgagag aaaccatgtt ggaaattgct
     1861 tctggtgctc aaaatcttcg tgccaaggaa gttggtgcct atgaactgag agaagtaact
     1921 cgccaattta atgccatgtt ggatcagatt gatcagttga tggtagctat tcgtagccag
     1981 gaagaaacga cccgtcagta ccaacttcaa gccctttcga gccagattaa tccacatttc
     2041 ctctataaca ctttggacac catcatctgg atggctgaat ttcatgatag tcagcgagtg
     2101 gtgcaggtga ccaagtcctt ggcaacctat ttccgcttgg cgctcaatca aggcaaggac
     2161 ttgatttgtc tctctgacga aatcaatcat gtccgccagt atctctttat ccagaaacaa
     2221 cgctatggag ataagctgga atacgaaatt aatgaaaatg ttgcctttga taatttagtc
     2281 ttacccaagc tggtcctaca accccttgta gaaaatgctc tttaccatgg cattaaggaa
     2341 aaggaaggtc agggccatat taaactttct gtccagaaac aggattcggg attggtcatc
     2401 cgtattgagg atgatggcgt tggcttccaa gatgctggtg atagtagtca aagtcaactc
     2461 aaacgtgggg gagttggtct tcaaaatgtc gatcaacggc tcaaacttca ttttggagcc
     2521 aattaccata tgaagattga ttctagaccc caaaaaggga cgaaagttga aatatatata
     2581 aatagaatag aaactagcta actcccagtc aattctggga gttttatgcg taattgggca
     2641 aactttctag gttgtctatc ttgctaaaaa caagaaaaaa cggtatgata gaagagtgac
     2701 aacagaggta acagagatga acgagacaca gattcaaaga gaaacacgtc aggtagtgga
     2761 agatgtttta gaaaagacca atttgaagca gggggccctt tttgttttag gtctatcttc
     2821 tagtgaggtg ctaggtggtc agattgggaa agaatccagt caagagattg gtgagctgat
     2881 agtggagact atcttggata ttctaggtag tagaggtatc catttagcag ttcaaggttg
     2941 tgagcatgtc aatcgggctc tcgttgttga acgtcaggtg gcagagcagt ttggtctgga
     3001 aattgtcagt gtccatccta ctcttcatgc aggaggttcg gggcagttgg cagcctttaa
     3061 gtttatgcag gatccagttg aggttgaatt tatcaaggct catgctggat tggatatcgg
     3121 agacactgca attggcatgc atgtcaagca tgttcaggtt ccgattcgcc ctattttgag
     3181 agagattggt catgcccatg taacggcact ggctagtcgt ccaaaattaa tcggaggtgc
     3241 gcgtgcgcac tatccgcaag atgctattag aaagacctga gaatgaataa aggagataaa
     3301 agtgtttaaa aaagaccgtt tttcaattcg taagattaag ggagttgtag gctctgtatt
     3361 tcttggaagc cttttgatgg ctccttctgt agtggacgca gccacctatc actatgtaaa
     3421 taaagagatt atttcacaag aagctaaaga tttaattcag acaggaaagc ctgacaggaa
     3481 tgaagttgta tatggtttgg tgtatcaaaa agatcagttg cctcaaacag ggacagaagc
     3541 atctgttttg acagcttttg gttatctatc aggtgacata ctcaaaacgc ttggcttgga
     3601 cactgtttta gaagaaacct cagcaaaacc tggagaggtg actgtagtcg aagttgagac
     3661 tcctcaatca acaacaaatc aggagcaagc taggacagaa aaccaagtag tagagacaga
     3721 ggaagctcca aaagaagaag cacctaaaac agaagaaagt ccaaaggaag aaccaaaatc
     3781 ggagataaaa cctactgacg acacccttcc taaagtagaa gaggggaaag aagattcagc
     3841 agaaccagct ccagttgaag aagtaggtgg agaagttgag tcaaaaccag aggaaaaagt
     3901 agcagttaag ccagaaagtc aaccatcaga caaaccaaca gaggaaccaa aagttgaaca
     3961 agtaggtgaa ccagtcgagc caagtgaaga cgaacaggca ccaacggcac cagttgagcc
     4021 agaaaagcaa ccagaagctc ctgaagaaga gaaggctgta gaggaaacac cgaaaccaga
     4081 agataaaata aagggtattg gtactaaaga accagttgat aaaagtgagt taaataatca
     4141 aattgataaa gctagttcag tttctcctac tgattattct acagcaagtt acaatgatct
     4201 tggacctgtt ttagaaactg caaaaggtgt ctatgcttca gagcctgtaa aacagcctga
     4261 ggtaaatagt gagacaaata aacttaaaac ggctattgac gctctaaacg ttgataaaag
     4321 tgaattacag gaacaattaa gagttgctga acaaaaacaa caagctgact atagcgctaa
     4381 aacttggagg gaattcaaaa ttgctgaact tcaagctaaa gaaatcaaca acccaactac
     4441 accacttcct aaacaaagtg aaatagatgc agctactaag gctcttcaag acgctcttca
     4501 agctcttgct gtagataaaa ctgtattaca aaacgctatt aacacagcta atagcaaacg
     4561 tgaagaagag tatactgctc agacatggaa agcgttaaaa gatgctctta cagcagtgaa
     4621 tcctgttaat gaagatgaaa ctgcaactca aagcaaagta gacgaagcta ctagaaactt
     4681 agaagaagct atcaataacc tagttctatt aactgaaaaa ccggtattaa catttattga
     4741 gactgataag aaagcattag aacgtgaagt agttgctaag tattctctag aaaaccaaaa
     4801 taaaacaaaa attaaatcaa tcacagctac tctgaaaaaa ggagaaacag ttgttagcac
     4861 tgtagagctt ataggcgatg atgtaactaa tgaaactata acctctgcat ttaagaatct
     4921 agagtactac aaagaataca ccctatctac aactatggtt tacgatagag gtgacggtga
     4981 tgtaacagaa atcttagata atcaaccaat ccaactagat cttaaaaaag ttgagcttaa
     5041 aaatattaaa cgtacagatt taatcaaata cgaaaatgga aaagaaacta atgaatcact
     5101 aataacaact gttcctgatg ataagcggaa ttattattta aaaataactt caaagaatca
     5161 aaaaactaca ttactagctg ttaaaaatat agaagaaact acggttaacg gaacacctgt
     5221 atataaagtt acagcaatcg cagacaattt agtctctaga actgctgata ataaatttga
     5281 agaagaatac gttcactata ttgaaaaacc taaagtccac gaagataatg tatattataa
     5341 cttcaaagaa ttagtggagg ctattcaaaa cgatccttca aaagaatatc gtctgggaca
     5401 atcaatgagc gctagaaatg ttgttcctaa tggaaaatca tatatcacta aagaatttac
     5461 aggaaaactt ttaagttctg aaggaaaaca atttgctatc actgagttgg aacatccatt
     5521 atttaatgtg ataacaaacg caacgataaa taatgtgaat tttgaaaatg tagagataga
     5581 acgttctggt caagataata ttgcatcatt agccaatact atgaaaggtt cttcagttat
     5641 tacaaatgtc aaaattacag gcacactttc aggtcgtaat aatgttgctg gatttgtaaa
     5701 taatatgaat gatggaactc gtattgaaaa tgttgctttc tttggcaaac tacactctac
     5761 aagtggaaat ggctctcata cagggggaat tgcaggtaca aactatagag gaattgttag
     5821 aaaagcatat gttgatgcta ctattacagg aaacaaaaca cgcgccagct tgttagttcc
     5881 taaagtagat tatggattaa ctctagacca tcttattggt acaaaagctc tcctaactga
     5941 gtcggttgta aaaggtaaaa tagatgtttc aaatccagta gaagttggag caatagcaag
     6001 taagacttgg cctgtaggta cggtaagtaa ttctgtcagc tatgctaaga ttatccgtgg
     6061 agaggagtta ttcggctcta acgacgttga tgattctgat tatgctagtg ctcatataaa
     6121 agatttatat gcggtagagg gatattcgtc aggtaataga tcatttagga aatctaaaac
     6181 atttactaaa ttaactaaag aacaagctga tgctaaagtt actactttca atattactgc
     6241 tgataaatta gaaagtgatc tatctcctct tgcaaaactt aatgaagaaa aagcctattc
     6301 tagtattcaa gattataacg ctgaatataa ccaagcctat aaaaatcttg aaaaattaat
     6361 accattctac aataaagatt atattgtata tcaaggtaat aaattaaata aagaacacca
     6421 tctaaatact aaagaagttc tttctgttac cgcgatgaac aacaatgagt ttatcacaaa
     6481 cctagatgaa gctaataaaa ttattgttca ctatgcggac ggtacaaaag attactttaa
     6541 cttgtcttct agcagtgaag gtttaagtaa tgtaaaagaa tatactataa ctgacttagg
     6601 aattaaatat acacctaata tcgttcaaaa agataacact actcttgtta atgatataaa
     6661 atctatttta gaatcagtag agcttcagtc tcaaacgatg tatcagcatc taaatcgatt
     6721 aggtgactat agagttaatg caatcaaaga tttatattta gaagaaagct tcacagatgt
     6781 taaagaaaac ttaacaaacc taatcacaaa attagttcaa aacgaagaac atcaactaaa
     6841 tgattctcca gctgctcgtc aaatgattcg tgataaagtc gagaaaaaca aagcagcttt
     6901 attactaggt ttaacttacc taaatcgtta ctatggagtt aaatttggtg atgttaatat
     6961 taaagaatta atgctattca aaccagattt ctatggtgaa aaagttagcg tattagacag
     7021 attaattgaa atcggttcta aagagaacaa cattaaaggt tcacgtacat tcgacgcatt
     7081 cggtcaagta ttggctaaat atactaaatc aggtaattta gatgcatttt taaattataa
     7141 tagacaattg ttcacaaata tagacaatat gaacgattgg tttattgatg ctacagaaga
     7201 ccatgtctac atcgcagaac gcgcttctga ggtcgaagaa attaaaaatt ctaaacatcg
     7261 tgcattcgat aatttaaaac gaagtcacct tagaaatact atactcccac tactgaatat
     7321 tgataaagca catctttatt taatttcaaa ttataatgca attgcctttg gtagtgcaga
     7381 gcgattaggt aaaaaatcat tagaagatat taaagatatc gttaacaaag ctgcagatgg
     7441 ttatagaaac tattatgatt tctggtatcg tctagcgtct gataacgtta aacaacgact
     7501 actaagagat gctgttattc ctatttggga aggttataac gctcctggtg gatgggttga
     7561 aaaatatggc cgctataata ccgacaaagt atatactcct cttagagaat tctttggtcc
     7621 tatggataag tattataatt ataatggaac aggagcttat gctgctatat atcctaactc
     7681 tgatgatatt agaactgatg taaaatatgt tcatttagaa atggttggtg aatacggtat
     7741 ttcagtttac acacatgaaa caacacacgt caacgaccgt gcgatttact taggtggctt
     7801 tggacaccgt gaaggtactg atgctgaagc atatgctcag ggtatgctac aaactcctgt
     7861 tactggtagt ggatttgatg agtttggttc tttaggtatt aatatggtat ttaaacgcaa
     7921 aaatgatggg aatcagtggt atattacaga tccaaaaact ctaaaaacac gagaagatat
     7981 taatagatat atgaagggtt ataatgacac tttaactctt cttgatgaaa ttgaggctga
     8041 atctgtgatt tctcaacaaa ataaagattt aaatagtgca tggttcaaaa aaatagatag
     8101 agaataccgt gataacaata aattaaatca atgggataaa attcgaaatc taagtcaaga
     8161 agagaaaaat gaattaaata ttcaatctgt taatgattta gttgatcaac aattaatgac
     8221 taatcgcaat ccaggtaatg gtatctataa acccgaagca attagctata acgatcaatc
     8281 accttatgta ggtgttagaa tgatgaccgg tatctacgga ggtaatacta gtaaaggtgc
     8341 tcctggagct gtttcattca aacataatgc ttttagatta tggggttact acggatacga
     8401 aaatgggttc ttaggttatg cttcaaataa atataaacaa caatctaaaa cagatggtga
     8461 gtctgttcta agtgatgaat atattatcaa gaaaatatct aacaatacat ttaatactat
     8521 tgaagaattt aaaaaagctt acttcaaaga agttaaagat aaagcaacga aaggattaac
     8581 aacattcgaa gtaaatggtt cttccgtttc atcatacgat gatttactga cattgtttaa
     8641 agaagctgtt aaaaaagatg ccgaaactct taaacaagaa gcaaacggta ataaaacagt
     8701 atctatgaat aatacagtta aattaaaaga agctgtttat aagaaacttc ttcaacaaac
     8761 agatagcttt aaaacttcaa tctttaaata attgaaatct ctcatctgct ttgcggatga
     8821 gggttttctt attttatgct atacttaaag tatgcataga aaaacagtga ttgattttag
     8881 gactttgggg gagagatata cctttaccca gcctattaaa gagttgaaaa cgagaaatgt
     8941 agaagaagtg gcagattttc tggcacaagt ggaaagctac caagagcaag gttattatgt
     9001 ggtggggtat gtcagctacg aggctgcacc tgcttttgag gaaaaattag cagttcacaa
     9061 ggatccccta ctgggagagt acctgcttta ctttactgtt catgatagcg tagaaacttc
     9121 ccctattcct ctgacttatg aggatattga tttgccctca aattggcagg aagtgacgtc
     9181 tgcagcagac tatgaaaagg ccattgccca gatacaccat cacttgaggc agggagacac
     9241 ctatcaggtc aactacaccg tccaactcaa gcaaaagtta agtgccaatc cttttgccat
     9301 ctacaatcgt atggtggtag agcaggaggc gggctacaat gcctatgtgg aacatgacga
     9361 gatggcagtg atttccatga gcccagagct cttttttgag caaaatgatc gcgagttgac
     9421 aacacgacca atgaagggga cgactcagcg tggggtaact gaccaagaag atcttgaaca
     9481 ggccagttgg ttggaacagg atcccaaaaa tcgctctgaa aatatgatga ttgtggacct
     9541 cttgcgcaat gatatgaacc gtatttctga agttgggagc gagcacgtgg agcgtctgtg
     9601 ccaggtagag cagtattcaa ctgtttggca gatgacttcg accatcaaga gtcagttgcg
     9661 agaggatgtg gaccttgttg aaatcttccg ctcactcttt ccttgtggtt ccataacggg
     9721 tgcaccgaaa attgcgacaa tggagattat caaggacttg gagcctcaac cgcgtggagt
     9781 ctactgtgga acgattggtc tcttgcttcc aaatggacga cggattttta atgtggccat
     9841 tcgtaccatt caacttcaca aaggtcaagc catttatgga gttggcggag ggattacttg
     9901 ggatagcaca tgggaatctg aataccgaga ggttcatcaa aaggcagcag ttctttatcg
     9961 taaacaagct cgcttccaac tgattacgac aggggaaatc agccagaaga acctgctgtt
    10021 tgaagatcaa catctggaaa gactgagaaa agctagtcgt tattttgctt ttccttttga
    10081 tgcagaagac ttgggacaca agattgagga agagtgtcag gattgtgaag ctaatcgaga
    10141 ttaccgcttg cgaatcagcc ttagcaaatc tggagagata gaagtcaatc gtcaagtatt
    10201 aacccctctc agtacaagct tttgtcaggc ccaagtctgc cttcaggaag ctgctttgaa
    10261 tcaatccttt acctacttta aaaccactca ccgaccgcat ttgagcctag gagaacaaga
    10321 gaagatttac cacaataagt caggagaact gcttgaaacc tctataggaa atttggttct
    10381 gaaaatcgct ggaaaactct acacaccgcc tatccgactt ggaatcttgc caggaattta
    10441 ccgtcagtat ttgctagaaa caggacaggt agaagagaaa gtcttgacct tggcagattt
    10501 agcccaagca gaagctattt acggctgtaa tgcagtgaga ggcttgtatg aactaatcct
    10561 tgaggagaac tagatgaaac tgatattttt gcatgggtta gggcagtcag cagagtcttg
    10621 gaaagaagtt cggaatctac tgacagatta tccttctgag gctatcgagt tatttccttc
    10681 tggagttagt aactatcaac aagctaagga gcgagtttat cagcacctag ctcaagagac
    10741 agaacctttt gttttgattg gattgtcctt aggtgctgcc ctggcactag aactttccag
    10801 ttacgattta ccaaatcttc gagctttgat tctgtcagga tgcccgctta aattagcagg
    10861 gaatatccta ttttatcttc agttgctgat ttttaaacta cttcccaaaa gagtatttga
    10921 aaaacaggga gcagacaagg ctcttatggt cggagtttct gaggagttga agacacttga
    10981 tttaacggat atagcaggaa cttgccctta tccaacctta ctaatttgtg gtatcaaaga
    11041 taaaccgaat cttagttcta tgagaagtct tcataaacta atatcagaat cccaatttca
    11101 gattatccca gatggccctc atgtcttgaa taaggcaaaa ccaaaggagt ttgtagaaaa
    11161 gaccagaagt tttcttgaat tgctgaaata agtttgataa aataatattg aaatcgatat
    11221 cataattatg ggagaaagaa atgaataaac gtctattttt aaaaatgagt ctggtgacgt
    11281 tgccaatttt agccttgttt tcacaacctg tgttagcaga ggaaaacatt catttttcga
    11341 gttgtaagga agcttgggct aatggatatt cggatcttca tgagggagaa cctggttatt
    11401 ctgccaagtt agaccgcgat catgatggtg tggcttgcga attgaaaaat gctcctaagg
    11461 gtgcttttaa agcaaaacaa gcggctacaa ctcaaactga tacaacttca tccacagcaa
    11521 gtggttgggt taagcaggac ggttcttggt actattttga tggaaatgga aatctagtga
    11581 aaaatgcatg gcagggaaat tattacctga aagctgatgg taaaatggca cagagtgaat
    11641 ggatttatga ctcttcttat caagcttggt attatttgac atcagatggt tcttatgctt
    11701 acagtacatg gcaaggaaat tactatctaa aatcggatgg taaaatggct gtcaatgaat
    11761 gggttgatgg tggacgttat tatgttggcg ctgacggagt ttggaaggaa ggtcaagcaa
    11821 gtacagcttc ttctagtaat gatagcaata gtgaatattc tgctgcttta ggaaaggcaa
    11881 aaagttataa ttcgttattc cacatgtcaa aaaaacgtat gtatagacaa ttaacttctg
    11941 attttgataa attttcaaat gatgcagctc aatatgccat tgatcattta gatgattaac
    12001 agatactggt aggcgaaaaa attccttata attcatttac gcaaaccttt gcataagtga
    12061 atgtattttg atatactagt actgtaagca ttttcaatta ctacacaatg aaaaaggaga
    12121 atatgatgag tcaaaagatt attgggattg accttggtgg aacttctatc aaatttgcaa
    12181 tcttaacaac agcaggagaa atccaaggaa aatggtcaat caagaccaac attttggatg
    12241 agggaagtca tatcgttgat gatatgattg agtctattca acatcgtttg gacttgcttg
    12301 gattggcagc agcggacttc caaggcattg gaatgggatc accaggtgtg gttgaccgtg
    12361 acaaagggac tgttatcggt gcctacaact tgaactggaa aacccttcaa ccaatcaaac
    12421 aaaagattga aaaagctttg ggcattccat ttttcatcga taatgatgcc aacgtagcag
    12481 ctcttggtga gcgctggatg ggtgctggaa ataaccaacc agacgttgtc tttatgacac
    12541 tcggtactgg tgttggtggt ggtattgtcg cagaaggcaa attgcttcac ggtgttgctg
    12601 gtgcagcagg tgagcttggt catatcactg ttgattttga ccaaccaatt gcatgtactt
    12661 gtggtaaaaa aggctgcctt gagacagttg cttcagcaac agggattgtc aacttgactc
    12721 gtcgctatgc cgatgaatac gaaggcgatg cagccttgaa acgcttgatt gataatggag
    12781 aagaagtaac tgctaagact gtctttgatc tcgcaaaaga aggagacgac cttgctttga
    12841 ttgtttaccg taacttctca cgttacttgg gaatcgcttg tgctaacatt ggttcaatcc
    12901 taaacccatc aacaatcgtc atcggtggtg gtgtgtcagc tgcgggagaa ttccttctac
    12961 aaggtgttca aaaagtttac gatgaaaata gtttcccaca agtacgcaca tctactaaat
    13021 tagctcttgc aactctagga aatgacgctg gagttatcgg agcagcatca cttgtattgc
    13081 aataagataa tgagagagga aaatgctaac ctagagttag taatgttcct cttttatttt
    13141 ttatgctata ctagttagga caatgaatga ggtgaaacga aatgacaaaa gcagatacga
    13201 tttttaaaga gaatattgaa cgcattctaa aggaaggtgt tttctcagag caggctcgtc
    13261 ctaagtacaa ggatgggact gttgctaatt ccaagtatgt aactggtgcc ttttccgagt
    13321 acgacttatc taaaggagaa tttcctatca caaccttgcg tcccattgca atcaaatccg
    13381 ccatcaaaga agttctctgg atttaccaag accagtcaaa tagcttagaa gtgcttaatg
    13441 acaagtacaa tgttcactac tggaatgact gggaagttgg agacacggga accattggtg
    13501 agcgctatgg tgccgttgtt aagaaacacg acattatcaa taagcttctc aaacagttgg
    13561 aaaccaatcc ttggaaccgc cgcaatatta tttcgctctg ggattaccaa gctttcgaag
    13621 aaacagatgg gctgctcccg tgcgcctttc agaccatgtt tgatgtccgg cgtgttgatg
    13681 gggaaatcta tctggatgcg accttgaccc agcgctccaa tgatatgctg gtggcccacc
    13741 acatcaacgc tatgcagtat gtggctttgc agatgatgat tgccaaacat tttggctgga
    13801 aggttgggaa gttcttctac ttcatcaaca acctccatat ctatgataat caatttgaac
    13861 aagctcagga attgctccgt cgggagccgt caaactgcca accacgcttg gttttaaatg
    13921 ttcctgatgg gactaatttc tttgatatca aagcagaaga ttttgagttg gtggattatg
    13981 accctgttaa gccacagttg aagtttgacc tagctattta aaagaataga aaaaagaagt
    14041 tgagaataat cccaacttct tttgtttctt aacgtgatac gcggcgacga gctgcttttt
    14101 tacggttttc ttcgatgaaa gctgcttttt gctcttctgg ttcgattact ttctttttaa
    14161 atgcgtatac tgcacctgca acggcagcga cagttcctgc gacacctgtt acaagacctt
    14221 tagcgaatcc tttagccatg agtcttcctc ctttatattc tcaatcagcc agcctcctca
    14281 agaggtcaca tttttctgac tgaccttttt gtgttataat aatagtaacg aaaaaatggg
    14341 aatttttcaa ggaaaaaaga tgaaaacaaa aataattgtg attgttggac cgactgctgt
    14401 tggaaagacg gctctagcta ttgaagttgc aaagcgtttt aatggcgaag tggttagtgg
    14461 agatagccag caagtctatc gaggacttga tattgggacg gccaaggcta gtccagaaga
    14521 gcaggcagct gttcctcatc atttaatcga tgttagagag ataaccgagt cttactcggc
    14581 ttttgatttt gtttcagaag ctaagatgac tattgaggat attcacagcc gtggcaagct
    14641 agccattatc gccggtggga ctggacttta tatccagagc ttgctagaag gttaccacct
    14701 aggtggggag actcctcatg aggagatttt agcttatcga gctagtttgg agccatattc
    14761 agatgaggaa ttagcccatt tggtggagca agcaggcctt gagattcccc agtttaatcg
    14821 tcgtcgtgct atgcgtgcct tagaaattgc ccattttggt caggatttgg aaaatcaaga
    14881 gaccttgtat gaaccgctga ttatctgctt ggatgatgaa cgtagtcaac tttatgagcg
    14941 tatcaaccac cgagtggatt tgatgtttga ggctgggctt ttggatgagg ccaagtggct
    15001 gtttgaccat tcccctaatg tacaggctgc taaaggaatt ggctataagg aactctttcc
    15061 ttatttccgt ggagagcaga ccttggagga agctagtgag agtctcaaac aggctactcg
    15121 tcgttttgcc aagcgtcagc tgacctggtt ccgtaatcgc atgcaggtca ccttttatca
    15181 gattggagaa tctggtgtac aagaccgcat tttaagccag atagaggagt ttttagatga
    15241 ttgaaacgga gaaaaaagag gagcgagtcc tgctgattgg tgtggaattg cagggtatgg
    15301 acagttttga cctctccatg gaagaattgg ctagtttagc gaaaacggca ggggcagtcg
    15361 ttgtagataa ctacagacaa aaacgtgaaa aatatgattc caagaccttc gtcggctctg
    15421 gtaagttgga agagattgcg cttatggtgg atgcagaaga aatcactact gtcatcgtca
    15481 acaaccgtct gacaccaagg cagaatgtca atctagagga agttctcggt gttaaggtca
    15541 ttgaccgtat gcagttgatt ttggatatct ttgccatgcg ggctcgaagc catgaaggga
    15601 agctccaagt ccacctagcc caactcaaat acctcttgcc tcgcttggtt ggtcagggga
    15661 ttatgctcag ccgtcaggca gggggaattg gttcccgtgg tcctggtgaa agccaactgg
    15721 agctgaaccg tcgtagcgtt cgcaatcaaa tcacggatat cgagcgccag ctcaaggtgg
    15781 ttgagaaaaa tcgggcgact gtcagagaaa aacgtttgga gtctagcacc tttaagattg
    15841 gtttgattgg ttataccaat gctgggaaat caactatcat gaacatcttg accagtaaga
    15901 cccagtatga agcagatgag ctctttgcga ctctggatgc gacaaccaag agtattcatc
    15961 tgggaggcaa tctccaagta actttgacag ataccgttgg ctttatccaa gatttgccga
    16021 cagagttggt gtccagtttc aagtcaacct tggaagaaag caagcatgtg gaccttctgg
    16081 ttcatgttat cgatgctagc aatccttatc acgaggagca tgaaaaaacg gttctctcca
    16141 tcatgaaaga cctggacatg gaagatattc ctcacttgac gctttataat aaagcggatt
    16201 tggtggagga tttcacgcct acccaaacgc catataccct catttctgcc aagtctgagg
    16261 acagtcgtga aaacttgcaa gcattattgc tagataagat taaggaaatt tttgaagcat
    16321 ttaccctgcg agtgcctttt tcaaagtcct acaagattca tgatttagag agtgttgcaa
    16381 ttctggaaga acgtgattat caggaagaca gcgaagtgat tacaggctac atttccgaaa
    16441 aaaataaatg gaggttagaa gaattttatg actgatatta aagctttggc tctaaagtat
    16501 ggaggttata caagtctgga caaggtctat ctggatcagc ttctagctgg taaaacagag
    16561 caggagcagt taacacttat tacacctccg ccaagtgttg ttaatgccta ctttgcagaa
    16621 ctctatcaga aaaagagtcc tcaggctgcg acggattatt ttgcagaact cagtcaggaa
    16681 ctgaatcttt acaatactga gccaagtttc accctagaaa ataagccttt tattcggctt
    16741 aatctgtctg gtaaatcctt tggtttttgt tatgatagtg acggccttgg tcggattttc
    16801 tctgaaaatg aggagaaaat ctcggatgac ttgctctttg aaattgcgca aattttcccc
    16861 catcaattag tctttgaaga gtctggtaag atttacatga agcctgtcga ggacgaggaa
    16921 gttgtcagcc tagaaagtct cacagctttg actgatttgg aaagattggc tgatggtcga
    16981 aaacgtctca aaggctacag ccaagaggat ttattgcaag aagctgctgc tttttctggc
    17041 aaacgctatt tccgatcgga aaaccgcaca gccatgttat atattgatta attagaaagt
    17101 atcgaatgga tattcaattt ttaggaacgg gggctggtca gccctctaaa gctcgcaacg
    17161 tttcaagtct cgccctgaaa cttttggatg agattaacga agtttggctc tttgactgtg
    17221 gagaaggtac gcaaaatcgc attctggaaa ccacaattcg accacgtaag atcagcaaaa
    17281 tctttattac ccatctgcat ggagaccaca tttttggttt gccaggtttc ctttctagcc
    17341 gtgcctttca ggccaatgaa gagcagacag atttggaaat ctacggacct caaggaatca
    17401 agtcatttgt cttaaccagc cttcgtgtgt caggttctcg tctgccctac cgcattcatt
    17461 tccatgagtt tgaccaagat tctctgggta aaattcttga aaccgataaa ttcactgtgt
    17521 atgcagagga gctggatcac actattttct gtgttggcta tcgtgtcatg caaaaagatc
    17581 tagaagggac gctggatgct gaaaaactca aggctgctgg tgttccgttc ggcccgcttt
    17641 ttggtaaaat caaaaacggc caggatcttg ttttggaaga cggaactgaa atcaaggcag
    17701 cagactatat ctcagcgcca cgtccaggta agattatcac tattttagga gacactcgaa
    17761 aaacggatgc cagtgtgcgt ctggctgtca atgcagatgt cctagttcat gagtccactt
    17821 atggcaaggg tgatgaaaaa attgctcgta accatggtca ctcaactaat atgcaagctg
    17881 cacaagtagc ggtagaagca ggtgccaaac gcctcctact caaccatatc agtgcccgtt
    17941 tcctctcaaa agatattagc aaactcaaga aagacgctgc cacaattttt gaaaatgtcc
    18001 atgtggtcaa agacttggaa gaagtggaaa tctagcagtc acagaaagga taagtatgcc
    18061 tactattctc attaccggag ctagcggtgg tctagctcaa gaaatggtca aactcctgcc
    18121 caatgaccaa ctcatcttgc ttggtagaaa taaggaaaaa ttagcccaac tctacggaaa
    18181 ttattcccat gcagaattga ttgaaattga tattaccgac gattcagccc tagaagctct
    18241 ggtaactgat ctttatctcc gctatggcaa gattgatgtc ttgattaaca acgctggtta
    18301 cgggattttt gagggatttg accagattgc tgataaagat attcaccaga tgtttgaggt
    18361 caataccttt gccctgatga atctgtctcg tcaccttgcg gctcgtatga aggaaagcag
    18421 caaagggcat atcatcaaca tcgtcagcat ggcaggtcta atagctactg gcaagtctag
    18481 tctttactca gcgaccaagt ttgcggctat tggtttttca aatgctctgc gactcgaact
    18541 tatgccctat ggagtctatg tgacaacagt caatccaggt ccaatccgaa caggattttt
    18601 tgaccaagct gacccagatg gaacttatct taaatcggtt gaccgcttcc tcttagaggc
    18661 agatgcagtg gctaaaaaga ttgtcaagat tataggcaaa aataaacgag aactcaatct
    18721 cccgattttg ttgaacctag cccataagtt ttatactctc tttcccaagc tagctgataa
    18781 gttggtaggg gaaactttta attataagta aaaag
//
LOCUS       DAVKGU010000032        18059 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKGU010000032 DAVKGU010000000
VERSION     DAVKGU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18059
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.032"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            115..1596
                     /locus_tag="VW077_001071"
     CDS             115..1596
                     /locus_tag="VW077_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9455101.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1958..2959
                     /locus_tag="VW077_001072"
     CDS             1958..2959
                     /locus_tag="VW077_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9455102.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3109..4026
                     /locus_tag="VW077_001073"
     CDS             3109..4026
                     /locus_tag="VW077_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9455103.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4037..4927
                     /locus_tag="VW077_001074"
     CDS             4037..4927
                     /locus_tag="VW077_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9455104.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4956..6572
                     /locus_tag="VW077_001075"
     CDS             4956..6572
                     /locus_tag="VW077_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9455105.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6582..7901
                     /locus_tag="VW077_001076"
     CDS             6582..7901
                     /locus_tag="VW077_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9455106.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVATKHKDNVGCIVLLGSDNLVEWQFESIFLKGREHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8109..8181)
                     /locus_tag="VW077_001077"
     tRNA            complement(8109..8181)
                     /locus_tag="VW077_001077"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8146..8148),aa:Lys,seq:ctt)
     gene            complement(8445..9716)
                     /locus_tag="VW077_001078"
     CDS             complement(8445..9716)
                     /locus_tag="VW077_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9455107.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            9986..10225
                     /locus_tag="VW077_001079"
     CDS             9986..10225
                     /locus_tag="VW077_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455108.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            10390..10569
                     /locus_tag="VW077_001080"
     CDS             10390..10569
                     /locus_tag="VW077_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455109.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10712..10885
                     /locus_tag="VW077_001081"
     CDS             10712..10885
                     /locus_tag="VW077_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9455110.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            10922..11374
                     /locus_tag="VW077_001082"
     CDS             10922..11374
                     /locus_tag="VW077_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9455111.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11665..12135
                     /locus_tag="VW077_001083"
     CDS             11665..12135
                     /locus_tag="VW077_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9455112.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            12154..13242
                     /locus_tag="VW077_001084"
     CDS             12154..13242
                     /locus_tag="VW077_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9455113.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLVPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            13223..13651
                     /locus_tag="VW077_001085"
     CDS             13223..13651
                     /locus_tag="VW077_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9455114.1"
                     /translation="MELEISDFLGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKIYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            13789..14739
                     /locus_tag="VW077_001086"
     CDS             13789..14739
                     /locus_tag="VW077_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9455115.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            14741..15484
                     /locus_tag="VW077_001087"
     CDS             14741..15484
                     /locus_tag="VW077_001087"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9455116.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLTQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15495..17291
                     /gene="pepF"
                     /locus_tag="VW077_001088"
     CDS             15495..17291
                     /gene="pepF"
                     /locus_tag="VW077_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9455117.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGVDVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQINLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEATIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(17351..18028)
                     /locus_tag="VW077_001089"
     CDS             complement(17351..18028)
                     /locus_tag="VW077_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9455118.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
BASE COUNT         5594 a         3112 c         3927 g         5426 t
ORIGIN      
        1 tattatacga aataaaagat tttaggaaaa gtcgttatat catgctatac ctattctttg
       61 tggtataatt gcaagaggtt taatccgata atttataaag agagaaagat attcatgaga
      121 gatttattat ctaaaaaaag tcataggcaa ttagaattat tagaattatt atttgaacat
      181 aaacgttggt ttcatcgttc tgaactagca gagttattaa attgtacaga acgtgcagtc
      241 aaagatgatc tatcccatgt taaatctgct tttcctgact tgatttttca ttcttctact
      301 aatggtatac gcattattaa taccgatgat agtgatattg aaatggttta ccatcatttc
      361 tttaaacatt caactcattt ttcgatttta gaattcatct tctttaatga aggttgtcaa
      421 gctgagagta tttgtaaaga attttatatc agttcatctt cgctctatcg tattattagc
      481 caaatcaata aagtgattaa aaggcaattt caatttgaag tcagtctgac ccctgttcaa
      541 atcattggaa atgagagaga cattcgttac ttttttgcac aatatttttc agaaaaatat
      601 tatttcctag aatggccatt tgaaaatttt tcatcagagc cactatctca attgttagaa
      661 ttggtttata aggaaacaag ctttccaatg aatttgtcaa cgcatagaat gctaaagttg
      721 ctcctagtta cgaacctata tagaataaag tttggtcatt ttatggaagt agataaagat
      781 tcttttaacg accaaagttt ggatttttta atgcaggcag aaggaataga aggtgttgct
      841 cagagttttg aatcagaata caatatctct ttagatgaag aagttgtttg ccaattattt
      901 gtgtcttact ttcaaaaaat gtttttcata gatgaaagtc tctttatgaa atgcgtaaaa
      961 aaggatagtt atgttgaaaa atcttaccat ctattgagtg attttattga tcagatttca
     1021 gtcaagtatc agattgaaat ggagaataag gataatctga tttggcatct gcataatacc
     1081 gcacatctgt atcgtcaaga gttgtttact gagtttattt tatttgatca aaaagggaat
     1141 acaatcagga actttcaaaa tatttttcca aaatttgttt cagatataaa aaaagagctt
     1201 tctcactatt tagagactct tgaggtttgt tctagttcaa tgatggtaaa tcatctatct
     1261 tataccttca tcactcatac caaacatttg gtgattaatt tgctacaaaa tcagcctaaa
     1321 ctaaaggttt tagttatgag taattttgat cagtatcatg caaaattcgt tgcagagaca
     1381 ctttcttatt actgtagcaa taattttgaa ctggaagttt ggactgaatt agaattatca
     1441 aaggaatctt tagaagattc accttatgat atcattattt ctaattttat tattcctcct
     1501 attgaaaata agagactcat ctattcaaat aatataaaca cggtctcact catatacttg
     1561 ttaaatgcca tgatgtttat tcgattagat gagtaagcaa aaataacaaa aaaacgctat
     1621 ttcgcgtttg aactgtacct caaaagttag acagaaaaaa tctaattttt ggggtgtttt
     1681 tgttaagaaa agaccttcaa tcatttaagc aaaatgtaca tctagattta tagccctcta
     1741 aaactcttgc tttttttgta gaagatacga aaaaagccat aaaaccaagg cttttcctgt
     1801 tttatgtaga tgtttacaca tgaaaattga agtattttca aaagctaatt ggagtgattt
     1861 tattcatgat gggataaatg atctggccaa aaatgattga gaaatagttg cttaaaaagc
     1921 atttgcatga tactatatat tgaaaggagg tgataatatg gtagaacaaa gaaaatcaat
     1981 taccatgaaa gatgttgctt tagaagcagg agttagtgtt ggaactgttt cacgtgtaat
     2041 taataaagaa aaaggcatta aagaagtaac tttgaaaaaa gtggaacaag cgattaaaac
     2101 tttgaattac attccagatt actacgctag aggaatgaaa aaaaatcgaa cagaaacgat
     2161 tgcaatcatt gtaccaagta tctggcatcc cttcttttca gaatttgcta tgcatgtgga
     2221 aaatgaagtc tataagagaa ataacaaatt actcttatgt tctatcaatg gtacaaatag
     2281 agagcaagac tatctggaga tgttgcgtca taataaagtt gatggagtgg ttgccattac
     2341 ctatagtcca attgaacatt acttgacgtc aggaattccc tttgttagta ttgaccgcac
     2401 atactcagat attgccattc cttgtgtttc atcagataat gatgcaggtg gaagagaagc
     2461 agctaaacaa ttaattagta aaggatgtca gcatttagcc tttgtgggcg gacataatac
     2521 aaccattaat gaaaccaaac gtcgtagaat ttcatttgaa aagtatgtcc aagagcataa
     2581 aatacctctt agtatttttg atttggatga gacagttact gactattatg gaaagctaga
     2641 gagtttcttg gaagaaaatt cttctataga tggaattttt acaatcaacg attttacagc
     2701 tttggatgtg attgaaatac taaaaaaaaa tggcaaacgt attcctcatg atgtccaaat
     2761 tattggatac gatggaatta aaatggctgg agatagagat tatctacttt caactattga
     2821 acaaccattg gaagaaatgg caaaagaagc ggttcgtatt ttgtttgata taattgatgg
     2881 aaagactgtt aatttgcaga caatcctacc agtaaaattt gttgaaggaa aaacaacaaa
     2941 aaatgaaaat aagtcttgac agaaagcgct atcaatgata gaatgaattc agataaaaag
     3001 atttattttt aaaacaaaaa tgaaacgttt caaaaaagaa ataaaaagac agcgccaagc
     3061 gctatctttt ctagaaaaaa atgaaacgtt tcaaaaaagg gggttgctat gaatagcaaa
     3121 gcgaagcaag tttctctttg ggaaagaatc aagaaacaaa aactcttgtt attgatgact
     3181 gtccccggtt tagttttaac ctttatcttt aaatacatcc ctatgtatgg ggttttaatc
     3241 gcatttaaag attacaatcc tttaaaagga attttaggga gtgattggat tggtttttct
     3301 gagtttacaa aattcatatc ctctcccaac tttggtatct tgttagccaa cacattaaaa
     3361 ttaagtatct atggtttatt gcttggcttt ttaccaccaa tcattcttgc gattatgctc
     3421 aatcaactct tgagtgaaaa agtcaaaaaa cgaattcagc tcattttata cgcaccaaac
     3481 tttatctcag tcgttgttat tgtcggtatg attttcctct tcttttcagt gggaggacca
     3541 atcaacaatt ttctttctat gtttggaatg aaggctgact tcttgacaaa tccagacttc
     3601 tttagaccct tatacatctt tagtggtatc tggcaaggaa tgggctgggc ttcaacgctc
     3661 tacacggcaa cattggtaaa tgtagatcca gccttagtag aagcagctcg actggatgga
     3721 gccaatatct tccaacgaat ctggcacatt gatattccag ctcttaagcc tattatggtt
     3781 atccaatttg ttttagctgc aggtggaatt atgaatgtcg gatatgaaaa agcattcttg
     3841 atgcagacat cgttaaattt gccaacttct gaaattatct cgacatatgt ctataaagtt
     3901 ggtcttgtat caggagacta ttcttactca acagcggttg gtttgtttaa tgcagtgatt
     3961 aacgtagtat tgcttgttgc agttaaccaa atcgttaaac gcatgaataa tggtgaagga
     4021 atttaaggag gaaagtatga aaaattcgat tatggataca aaatttgata gacgtatctt
     4081 actcttaaat aaaatcatta ttgtctttat cgttttgatg actttgcttc ctttacttta
     4141 tatcgtcgta gcatccttta tggatcctaa ggttctggtt agtagaggga ttagctttaa
     4201 tccagccgat tggactgtag aaggttacca gcgtgtattc agtgaccaat ctattctaag
     4261 aggttttatc aattctctac tatactcttt tggatttgca gctttaacag tcttgctatc
     4321 tgtgtttaca gcttatcctc tttccaaaaa agatttggta gggcgccgtt ggattaacta
     4381 cttcttgatt gtgactatgt tctttggtgg tggtttagtc ccaacttact tgctcgtaaa
     4441 agaattggga atgctcaata ctccatgggc tatcattgtt ccaggtgctg tcaacgtttg
     4501 gaatattatt cttgctaggg cctatttcca aggattgcct gaagaattag ttgaagctgc
     4561 tgtcattgat ggtgcaaatg atttacagat tttcttcaaa atcatgcttc ctcttgcaaa
     4621 accaattatg tttgttctct tcctttatgc ttttgtagga cagtggaact catactttga
     4681 tgcaatgatt tatatcaagg atccaaactt ggaaccattg caacttgtac ttcgtaaaat
     4741 tctcattcag agccaaccag gtcaagacat gattggagca caagcggcta tgaatgaaat
     4801 gaaacgttta gctgaattga ttaaatacgc aactattgtc atttccagct tgccattgat
     4861 tgttatgtat ccattcttcc aaaaatactt tgataaagga attatggctg gttcacttaa
     4921 aggataaaaa aagaaaaaat aaaaggagtt ttctcatgaa attcaaaaca ttctcaaaat
     4981 cagcagtttt gttgacagct agtttagcag tacttgcagc ctgtggctca aaaaatacag
     5041 cttcaagtcc agattataag ttggaaggtg taacattccc gcttcaagaa aagaaaacat
     5101 tgaagtttat gacagccagt tcaccgttat ctcctaaaga cccaaatgaa aagttaattt
     5161 tgcaacgttt ggagaaggaa actggcgttc atattgactg gaccaactac caatccgact
     5221 ttgcagaaaa acgtaacttg gatatttcta gtggtgattt accagatgct atccacaacg
     5281 acggagcttc agatgtggac ttgatgaact gggctaaaaa aggtgttatt attccagttg
     5341 aagatttgat tgataaatac atgccaaatc ttaagaaaat tttggatgag aaaccagagt
     5401 acaaggcctt gatgacagca cctgatgggc acatttactc attcccatgg attgaagagc
     5461 ttggagatgg taaagagtct attcacagcg tcaacgatat ggcttggatt aacaaagatt
     5521 ggcttaagaa acttggtctt gaaatgccaa aaactactga tgatttgatt aaagtcctag
     5581 aagctttcaa aaacggggat ccaaatggaa atggagaggc tgatgaaatt ccattttcat
     5641 ttattagtgg taacggaaac gaagatttta aattcctatt tgctgcattt ggtatagggg
     5701 ataacgatga tcatttagta gtaggaaatg atggcaaagt tgacttcaca gcagataacg
     5761 ataactataa agaaggtgtc aaatttatcc gtcaattgca agaaaaaggc ttgattgata
     5821 aagaagcttt cgaacatgat tggaatagtt acattgctaa aggtcatgat cagaaatttg
     5881 gtgtttactt tacatgggat aagaataatg ttactggaag taacgaaagt tatgatgttt
     5941 taccagtact tgctggacca agtggtcaaa aacacgtagc tcgtacaaac ggtatgggat
     6001 ttgcacgtga caagatggtt attaccagtg taaacaaaaa cctagaattg acagctaaat
     6061 ggattgatgc acaatacgct ccactccaat ctgtgcaaaa taactgggga acttacggag
     6121 atgacaaaca acaaaacatc tttgaattgg atcaagcgtc aaatagtcta aaacacttac
     6181 cactaaacgg aactgcacca gcagaacttc gtcaaaagac tgaagtagga ggaccactag
     6241 ctatcctaga ttcatactat ggtaaagtaa caaccatgcc tgatgatgcc aaatggcgtt
     6301 tggatcttat caaagaatat tatgttcctt acatgagcaa tgacaataac tatccaagag
     6361 tctttatgac acaggaagat ttggacaaaa ttgcccatat cgaagcagat atgaatgact
     6421 atatctaccg taaacgtgct gaatggattg taaatggcaa tattgatact gagtgggatg
     6481 attacaagaa agaacttgaa aaatacggac tttctgatta cctcgctatt aaacaaaaat
     6541 actacgacca ataccaagca aacaaaaact agaggttgat tatgggagat aagaaataca
     6601 cagtagaaaa agccaatcgt tttatagcag aaaataaaca tctcgttaat actcaatata
     6661 agcctgaaga acatttttca gctgagattg gttggatcaa tgatccaaat ggatttgtct
     6721 attttcgtgg agaataccat ctcttttatc aattctatcc atatgatagt gtttggggac
     6781 ctatgcactg gggacatgct aaaagtaagg acttggtgac ttgggagcac ttgccagtgg
     6841 cacttgctcc tgatcaagat tatgaccgaa atggttgttt ctcaggctct gccattgtca
     6901 aggatgatcg cctctggctc atgtacactg ggcatatcga agaaaaaacc ggtgtccgcc
     6961 aagtgcaaaa tatggcattt tcagacgacg ggattcactt tgaaaagatt gaacaaaatc
     7021 cagttgcaac aggagcagac ttgccagatg agttgattgc tgctgatttc cgtgatccaa
     7081 aactctttga aaaagatgga cgctattact ccgtagtagc taccaaacac aaggataatg
     7141 tgggctgtat cgttctacta gggtccgata acctagtaga atggcagttc gaatccatct
     7201 ttttaaaagg gagagaacac caaggcttta tgtgggaatg cccagattac ttcgagttag
     7261 atgggaaaga ttgccttatt atgtcaccca tgcgttatca gcgtgaggga gactcatatc
     7321 ataacatcaa ctcatcgctt ttgttcacgg gtaaggtaga ttggggagaa aaacgtttta
     7381 tcccagaatc agttcaagaa atcgatcatg gccaagactt ctatgcgcct caaacattgt
     7441 tggacgatca aaatcgtcgt atcctgattg cttggatgca gacatggggg cgtacccttc
     7501 caacccatga ccaagaacac aaatgggcat gtgccatgac tttacctaga attctaagat
     7561 tggaagatgg caaactaaga caattccctg ttaaaaaagg ccaatatcaa atccaaatag
     7621 ataaagattg tcattaccac ttaggaaatg atacagatta tcttgaattt ggttatgaca
     7681 gtaatgcgca gcaagtttac attgatcgta gccatcttat tcaaaaaatt ctaggtgaag
     7741 aagaacagga cactagtcga cggtatgtag atattgaagc taaagaattg gaagttgttc
     7801 tagataaaaa ttccatcgag atttttgtca atcaaggtga agcaagcttg actgcaactt
     7861 attacttaac ggtgccagct gagctatcac gaattgatta aaaattaagt tatttctcct
     7921 aaagaaaaag ttctctttct aaaatagtgg aaagaggact ttttgtgttt tgggtatata
     7981 agcttagttt atggtatttg taaaattggt gttggattat gatttaagct agttttctaa
     8041 agaatttgaa aaaaatttta tttaagcaaa aaaaccttgg ttccaaggct tttcctgttg
     8101 tatttagatg ccccctacag ggatcgaacc tgtgacccac ggattaagag tccgctgctc
     8161 tgccagctga gctaaggagg caaagaaaaa gctgtattgg tgccgaaact tcacggtttg
     8221 tattgaaccc gcgcaattaa gcaggtgggc aactcgctct aactgaagct gtttccgtgt
     8281 gagacggcct acatgctgtt agaagacttt tgtttcccta ataatacaaa aaatagtcgg
     8341 tcaacactta agtgtgaagt cgtacaccac agcgtttcta tacttatatg ataccacttt
     8401 ttcaaaaaaa atcaagagga aagtgcaatt ttttgaaaag gatttcagat ttttcgcaaa
     8461 cgatcgatag cttcctttct ttgagccaaa gcccgttcat atttaccagt atcttctgct
     8521 tggaaatagt gatgattacg aattttttct ggcagatagt cttgcttgac ccaatttcca
     8581 ggatagttgt gtggatagag atagtcttgg gcattcccca gttccttgct tccactgtag
     8641 tgcccatcac gcaggtgtcg cggaataggc aagtgccctg atgttttgag gtcagcaagt
     8701 gccttatcca tagctacata ggctgagttg gattttggag aaagggccaa atcaatcacg
     8761 acattggcaa tgagaatgcg ggcttcgggg aaaccaatcc tttgtgcagc atccagagca
     8821 gtcacggtat gaatctgggc ctcaggattg gctaagccga tatcttcata ggcgataaca
     8881 gtcaagcgac gagcgagact aggcagatcc ccagcctcaa tcaagcgggc agtatagtgg
     8941 agactggcat ccacatctga gccacgaata gacttttgca gggcagatag aacatcatag
     9001 tgaccgtctc catccttgtc catagtgatg tagcttctct gaagactatt ttccatgatg
     9061 tctaaggtga tgtggcgaat gccctcgtca ttctcagggg tagatagaac agccaagtcc
     9121 agtgagttaa aggcagagcg aaggtctccg tttgtagagg ttgcgatgaa atccagcgca
     9181 tcctcatcta gttctattgg aaaatcaaaa ccacgttcag ggttacttag agctatctgc
     9241 agggcctctt tgacgtcttg gttagacaga ggttccaact cgaaaatttg aactcggcta
     9301 cgaatggcag gagtgacaga gaagaaagga ttttcagtcg ttgctccaat catgatgacc
     9361 agtccacttt ccaagagagg caagaggaag tcttgcttgg tcttatctag tcgatgaatt
     9421 tcgtctagca ataggacgag accaccagaa aatttagctt cttccgagat ttcttgcagt
     9481 cgctttttac tatcaactgt cgcattgaaa gttcgaaagg catacttggt cgttccagcg
     9541 atggcagagg caatactggt tttgccgatt ccaggagggc catatagaat catggaggac
     9601 aggcggttgg cttccaccat gcggcggatg atttttccag gtccgaccag atgctcctga
     9661 ccgatgacct ggtcgatggt tttagggcgc atgcgaagcg cgagattgtc tggcatagca
     9721 gtcctttcta acgtggattt gagtagaatg actcatctac tatatcttac tatgtccatt
     9781 ctagcctatt agttgtgata tttcaatggt tttgtaataa tcagggagtg agatagaatc
     9841 ttttttacta tttaatttaa tgaggagagg tatcagcatg agagatttga ttttccttaa
     9901 agctcaaaat aggatagttg agcctaaaga agtggaagtg ctgacttgag aaataatatt
     9961 gctgaatgtc attgtatgaa ctataatgaa aagagaggtg ataagcaatg gaaatgatgg
    10021 accaagtcag gaaattttga tttttacaaa acaaattcgt cattggattc ttagtgatca
    10081 agttatttca gggaaaagaa aacttttctt ccgggaagat actccaaaag agattttaga
    10141 tttgtacgaa aacattaaat ctaaacttga ttttgcttac caagaagttt attctaataa
    10201 tgggttgaaa aaatatgaaa aatagaataa ttgatgtttt tgaagtagta aatcgcatcc
    10261 ttgtactaac tgttgaaaat cctgatttcg aagacttgag agttaatcat actcaatgaa
    10321 aatcaaagag caaactagga aactagccgc aggctgtact tgagtacggt aaggcgacgt
    10381 tgacgtggtt tgaatttgat tttcgaagag tatcagtttg taaaaattgg agataaaaaa
    10441 tatagagtac gcagtggtcc tatgattcat tcaactccgc ctcaatctgt cttagatcga
    10501 gatactttta caattgacta tacagatgat gaattgctcg ataaagaagc agtttttact
    10561 actcattaga aaaataggtc tgaataaatt ggtttccctt agaaccacat ctaggggctt
    10621 ttatgtataa tagaataata agtatcactc ctttacaata cgtgtaacac gtgttataat
    10681 ggtgttgtca ggaggtaaaa gcgctatacc tatgacacaa aaagagatgg ttaaactcct
    10741 tacggcccat ggttggataa agactagagg cggtaaaggc tcccatatta aaatggagaa
    10801 gcaaggggaa agacctatca caatccttca tggtgagctg aataagtaca ctgaaagagg
    10861 gataggaaag caagctgggt tgtaaaattc agtccctgct ttttgatatg gaggtagtga
    10921 aatgttagtt acgtatccag ctttatttta ttatgacgac acagacggaa cagaagcgac
    10981 ttattttgtc catttcccag attttgaata ctcagctaca caaggagagg ggatttctga
    11041 ggctttggct atggggtcgg agtggctagg gataactgtt gcagatttga tagagagtga
    11101 tggagagtta cctcagccat cagatatcaa tagcttgtct ttaattgata atgatccttt
    11161 taaagatgat gaagatttcg tgtcaaccta tgaccttgat aaatctttta tttctatggt
    11221 atctgttgat gtatcagaat acctaggaag tcaggaaccc attaaaaaga ctttgaccat
    11281 accaaaatgg gcagataagt tgggacgaga aatgggactt aacttttctc agactttgac
    11341 agacgctatt gcagataaga aagttcaagc ctaagattaa gcgtggtatc gtggtaatag
    11401 tcaataaaaa agcacgtcca cttgtgctag ttactcgcct attgatttta tagatttatt
    11461 agccctttcg cgggcttttt atattgattt ttatgagaaa tgaagaaatc agacttttaa
    11521 gaaaatcact gataccaagg gtttaaatga ggtaatatgg tataattagg acataaaata
    11581 attttgtggt aagatggtag tatctatttt agcatatttc cgagcaatcg gggcgattaa
    11641 agagtcgcat agaaagagga caaaatggca acatacggat ttttagatgt tttagaggaa
    11701 gagttggaca agaactttcc ctttgacttt gagattagct gggacaaacg caaccatgcg
    11761 gtagaagtga gttttctatt ggaagcacaa aatgctgcag gtgtggagat ggtggatgaa
    11821 gacggagagg tttcatcaga tgacattctt tttgaggaag cggtcctttt ctacaatcct
    11881 gctaagtcaa ccgtcaatgc agaagactat ttaacggtta tcccttatct acctaaaaaa
    11941 ggattttctc gcgagttttt agcttatttt gcccttttcc tcaaagatac tgcggaagtt
    12001 gggctagata ccctcatgga ctttttggaa gatccagaag cagaggaatt tgttatggaa
    12061 tggaatcaag aagtctttga agaaggaaaa gttggcttgg aagagggaga attttatcct
    12121 tatccaagat actaggagtt ggttggaggt gttatgaaga aaattgggac ttatttggtt
    12181 tatgtgctag ctgttgtctt tattatgcta gcttttgctt gtggaacaat cgcatttgca
    12241 gagttggggt attccgcagt tttagccttt acttttggtt atgccttcgc tcttctaagc
    12301 atgtatttaa tctttattct tcatgagctg ggtcatgctt tttgtggcta cttgacaggc
    12361 tatcggctgg tggcttttgg attaggacat tttattttga ccaaaaagtc aggcaggttt
    12421 catcttagta gaacagccat tctgaaaaat gttggtgctc aatacattgg tttaaaagag
    12481 gatgaaagcg atcaaagaat catcctgatg ctttcaggag gcttgatggt tcatctcggc
    12541 ttgatattat tggcgatagt gtttggattt ttgacaagaa gctggtattt tgcagggact
    12601 tggatttttc ttaatttatc tttcttccta aataacattt tgccagtcga catcaccgat
    12661 ggagcaaaaa tttgggaatt actacaacac cctgaaaata ccaaatacgc ctacctggta
    12721 ttgagacatt ctgctcaaac tttgctggtt cctcaagaat atgatttgaa agactttgtt
    12781 caagcagttc ctgaggatgc acgagggagt tttgcggatg gtgtattagg tatgcaaggt
    12841 gaagtttcca ttttggaagg gaaggaagaa gtagctaaac agcagtttca agcattactt
    12901 gagaggacaa ctactccgat gatgcagaca gttgcccaac tatccctgct tcatatcgcc
    12961 ttgttggaag gagattttga gcaagcggag caatatgcta gtattcgaca ggtcaaatcg
    13021 tttctgtctc tcaaactgtc caatcttcaa gtgatacaag cctggtatca attaaaggtg
    13081 aagaaagatg tagatcgaac tcgtaaggct ctcaagattg ctagacagaa aatggatagt
    13141 agtcgaatgt tgagggacga gaaacgctat tatgaaaact tgttagcaga gctggaaaag
    13201 gaaatgttag aaggaactga agatggaact agaaatttct gatttcctag gctgtaagat
    13261 tgctttgttt tgtggggata agcttttgac tatcttacgc gatgataagg caagcattcc
    13321 ctgggccaat atgtgggaac tgccaggtgg tggacgtgaa ggtgatgaaa gtccttttga
    13381 gtgtgtagcg cgtgaagttt atgaagaact gggcattcat ctgactgaag attgtctgct
    13441 ttggagtaag atttacccaa gtatgctttt tgcagacaaa cagtccgttt ttctagttgg
    13501 tcagttgaca cagaaccagt ttgacagtat cgtatttgga gatgaaggac agggctatca
    13561 gctgatgaat gttgaggaat ttcttagttc cagtcaagtt gtgcctcagt tgcaagagag
    13621 attaaaagat tatttaaaag taagtgatta gaaaggatgg ttcggttaaa tttctaaact
    13681 gaacccgccc taaacacggt gccaaaaaga taaacttctc ttagacacaa acgtcttcag
    13741 agaatttcct attttggttt tgtgttttac gggcttggta tcttaataat ggaaacatgg
    13801 caagagttaa aagttacagt gaagcgtgag ggagaggagt tggtttccaa tctcttgatt
    13861 gagctaggag cgcaaggagt cgcaatcgaa gacagtatgg actatatggg aaatgtggat
    13921 cgttttggcg agattttccc agaggtggag cagcaagaag aaatcgtagt gacagcctac
    13981 taccctgaca cggtggatgt gacagtggtt gaggcggact tgcaggctcg cttagcagaa
    14041 ttgacggatt ttatggattt gggtgaagtc aagatgggga cgactgcctt ggctgaggaa
    14101 gactgggcag acaactggaa gaaatactat gagccagctc gtatcactca tgatttgacc
    14161 atcgttccct cttggacaga ctatgaggcg actgcgggag aaaaaattat caagctagat
    14221 cctgggatgg cttttggtac tgggacccat ccaactacca agatgagcct ctttgccttg
    14281 gaacaggttc ttcgtggtgg cgagacagtg ctagatgtgg ggactggttc aggggttctc
    14341 tccattgcta gctcgctact tggtgccaag gaaatcttcg cctatgacct agatgatgta
    14401 gcagttcgtg ttgctcagga aaatattgaa cttaaccctg gaatggaaaa tatccacgtt
    14461 gctgctggag acttgcttaa gggtgttgag attgaggcag atgtgattgt agctaatatc
    14521 ttggcggata ttctcattca tctgacagac gatgcctatc gtttggtcaa ggatgaaggc
    14581 tacctgatca tgagtggcat tatcaaggac aagtgggaca tggtgcgcga gtcggctgag
    14641 tcagctggat ttttccttga aacccacatg gttcaagggg aatggaatgc ctgtgtcttt
    14701 aagaaaacca aggatatttc tggtgtgatt ggaggctagc atgcagcagt attttgtaaa
    14761 aggcagtgct atctctcctg tcactatcga ggacaaggaa accagtaagc atatgtttca
    14821 ggttatgcgc ttgaaagaag aggatgaggt taccttagtc tttgatgatg gcatcaagcg
    14881 cttggcgcgc gtgctggata tggaaaatcg tcagtttgag ttggtccaag aattaaatga
    14941 caatgtggaa ctaccagttc aagtgaccat cgcatctgga tttcccaagg gagacaagct
    15001 ggagttcatc gctcaaaaag taaccgaact gggtgctagc caaatctggg cctttcctgc
    15061 agactggtca gttgccaagt gggatggcaa gaaattgggt aaaaaagttg aaaaactaga
    15121 aaaaattgcc cttggagcag ccgagcaaag caagcgtaat attgtaccaa gtattaaact
    15181 tttcgagaaa aaagcagatt ttctaactca gctggaccag tttgactcta tcatagtagc
    15241 ctatgaagaa tcagctaaag aaggagaagc cgctgcgctt ctgcaagcag tcactggtct
    15301 tgaaacagga gccaaactgc tctttatctt tggtccagaa ggcggtctct cacctgcaga
    15361 aatcgaaagt tttgaagcta agggagccgt tttggtaggc ctaggtcctc gtattttgcg
    15421 agcagaaaca gcaccgcttt acgccttatc agcccttagt gttttagtag aattagagaa
    15481 ataagaggaa gaaaatggaa caaaaacacc gttcagaatt tccagagaag gaactttggg
    15541 atttaacagc tctataccaa gaccgtgagg atttcttgcg tgcaatcgag aaagctcgcg
    15601 aagacatcaa ccagtttagc cgtgattaca agggcaatct tcacactttt gaggatttcg
    15661 agaaggcctt tgcggaattg gaacagatct acattcagat gagccatatt ggcaactatg
    15721 cttttatgcc tcagacgacg gactatagca atgacgaatt tgcaaatatt gctcaagctg
    15781 ggatggaatt tgaaacagat gccagcgtag ccttgacctt ctttgacgat gccttggtgg
    15841 tagcagacga ggaagtcttg gaccgtttgg gtgaattgcc tcacttgacg gcagctattc
    15901 gtcaggccaa aatcaaaaaa gcccactatc taggggttga tgtggagaag gccttgacca
    15961 atctcggtga agttttctat agtccgcagg acatttatac taagatgcga gctggggatt
    16021 ttgaaatggc tgactttgaa gcccatggca agacctataa aaacagcttt gtcacctatg
    16081 agaatttcta ccaaaatcac gaggatgctg aggttcgtga gaaatccttc cgttccttct
    16141 cagagggact tcgtaagcac caaaatacgg ctgcagcagc ctatctggct caggtcaagt
    16201 ctgaaaaact cttggctgat atgaagggat acgactctgt ctttgattat cttctagctg
    16261 aacaagaagt ggaccgtgtc atgtttgacc gccagattaa cctcatcatg aaggactttg
    16321 caccagtcgc tcagagatac ctcaagcatg ttgccaaggt aaatggtctt gaaaagatga
    16381 cctttgcaga ctggaaattg gacttggaca gcgccctcaa tcctgaagcg actattgacg
    16441 atgcctatga tttggtcatg aagtcggtag aacctttggg gcaagaatat tgtcaggaag
    16501 ttgctcgtta ccaagaagag cgctgggtgg actttgctgc taacagtggc aaggattccg
    16561 gtggttatgc ggcggaccca tatcgcgtac acccttatgt actcatgagc tggacaggcc
    16621 gtttgagcga tgtctatacc ttgattcatg aaatcgggca ttctggtcaa ttcatctttt
    16681 cagacaatca ccaaagctat ttcaacgccc acatgtcgac ctattatgtt gaagcaccgt
    16741 cgaccttcaa tgaattgcta ctcagtgatt acttagagaa ccagtctaat gacccacgtc
    16801 aaaaacgctt cgctctggct catcgcttga cagacaccta cttccataat ttcatcaccc
    16861 acctcttgga agccgccttc cagcgtaagg tgtatacatt gattgaagaa ggagaaacct
    16921 tcggagcaag caaactcaac agcattatga aggaagtttt gacagatttt tggggcgatg
    16981 ccattgaaat cgacgacgat gcagctttga cttggatgcg ccaagctcac tactatatgg
    17041 gcttgtatag ttacacttac tctgctggtc ttgtcatctc gacagctggt taccttcatc
    17101 ttaaaaactc agaaactgga gctgaagact ggctcaatct cctcaaatca ggtggtagca
    17161 agacaccact tgagtcagcc atgattatcg gtgctgatat ttcaacagac aaaccactcc
    17221 gtgataccat ccaattcttg tctgacacag ttgaccagat tatcgcctac agtgctgagt
    17281 tgggggagta aggggataga gaggttgtta tagaatgggt atggacagtt atttgcttaa
    17341 aagtccatgt ttaaatcccc cattcctttt cgaggaattc tttgcccttc tctgtaatgg
    17401 taagtgaacg tggcgtttgt gcagggatga ccaattgttt ttcactgaaa aattctaaaa
    17461 gagcattgcc gagagctccg ccgacatgat ttttacgttc gctccaatcc aaacatttag
    17521 ggcaaaattt ccgttttttc tttttcaatt catccgtatc aatgtgtaat tttctttgga
    17581 aataagactt cccagcttca gtgagttgga agtctttttc tgattcgctg aggagatttt
    17641 cttgcaagag ataattggta atggaaatgc caagctcacc agcaatatgg tcatagcagg
    17701 tgcgagcgac ctttaattcc tttttgtgaa tgtgttgttt ataacttttg acatcacgct
    17761 gaggagagta gaagctgaga aattccagca tttctgcaat ggcagcactt tttaaagaat
    17821 aataatgaaa acgtccatag gtttgtagag tggttacctg cgcttcggta agtaatttga
    17881 ggtgatagct gacagtatgt tgcttgattt tggtaaagct ggcaatttcg ttaaccgtgt
    17941 gagcttttcc atccattaga atatctaaaa tgtgcatccg agaagggtct gataagagtt
    18001 ttgtgatgat gaaaatatct ggttccataa aaattttctc caaatttcat atatcgatg
//
LOCUS       DAVKGU010000033        17960 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKGU010000033 DAVKGU010000000
VERSION     DAVKGU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17960
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.033"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..84
                     /locus_tag="VW077_001090"
     CDS             <1..84
                     /locus_tag="VW077_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU9455119.1"
                     /translation="PILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            132..1607
                     /locus_tag="VW077_001091"
     CDS             132..1607
                     /locus_tag="VW077_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9455120.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            1630..1707
                     /locus_tag="VW077_001092"
                     /pseudo
     CDS             1630..1707
                     /locus_tag="VW077_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            1957..8659
                     /locus_tag="VW077_001093"
                     /pseudo
     CDS             1957..8659
                     /locus_tag="VW077_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(8907..9763)
                     /locus_tag="VW077_001094"
                     /pseudo
     CDS             complement(8907..9763)
                     /locus_tag="VW077_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9974..10333)
                     /locus_tag="VW077_001095"
     CDS             complement(9974..10333)
                     /locus_tag="VW077_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9455121.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            10425..11381
                     /locus_tag="VW077_001096"
                     /pseudo
     CDS             10425..11381
                     /locus_tag="VW077_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11365..11922
                     /locus_tag="VW077_001097"
     CDS             11365..11922
                     /locus_tag="VW077_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9455122.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            11915..12169
                     /locus_tag="VW077_001098"
     CDS             11915..12169
                     /locus_tag="VW077_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455123.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12181..14235
                     /locus_tag="VW077_001099"
     CDS             12181..14235
                     /locus_tag="VW077_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9455124.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14414..15274
                     /locus_tag="VW077_001100"
     CDS             14414..15274
                     /locus_tag="VW077_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9455125.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            15485..16195
                     /gene="ccdA2"
                     /locus_tag="VW077_001101"
     CDS             15485..16195
                     /gene="ccdA2"
                     /locus_tag="VW077_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9455126.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16215..16781
                     /locus_tag="VW077_001102"
     CDS             16215..16781
                     /locus_tag="VW077_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9455127.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            16792..17904
                     /gene="msrB"
                     /locus_tag="VW077_001103"
     CDS             16792..17904
                     /gene="msrB"
                     /locus_tag="VW077_001103"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9455128.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         5420 a         3409 c         3943 g         5188 t
ORIGIN      
        1 ccaatcttat tccgtatccc agcaatgagc gctccagtat ttgacaatat tgaagcagct
       61 attaaagaac acggattaag ctaattctaa tttgttaaca ttcatataac ataaaaaagg
      121 gaggaactgt tatggatgca atctttgacc taatcggaaa ggttttcaat cccatcttag
      181 aaatgggtgg acctgtcatc atgttaatca ttttgacagt attggcttta ctttttggag
      241 tgaaattctc caaagcgctt gaaggtggta tcaaacttgc catcgctctt acaggtatcg
      301 gtgctatcat cggtatgcta aatggtgctt tctcagcatc acttgcaaaa ttcgttgaaa
      361 acactggtat acaattgagt attaccgacg ttggttgggc accacttgca acaatcactt
      421 ggggttctgc ttggacactt tacttcttgc tcatcatgtt gattgtcaac gtagtgatgc
      481 ttgctatgaa gaaaaccgat acacttgatg tcgatatctt tgatatctgg cacttgtcta
      541 tcacaggtct tttgattaaa tggtatgctg ataacaatgg tgtgagtcaa ggggtttcac
      601 tcttgattgc tacagcagct atcgttcttg tcggtgtgtt gaaaattatc aactctgact
      661 tgatgaaacc tacatttgat gaccttctta acgccccaag ttcatcacca atgacatcaa
      721 ctcacatgaa ctacatgatg aacccagtta tcatggtttt ggataagatt tttgaaaaat
      781 tcttcccagg ccttgataaa tatgactttg atgctgctaa attgaacaag aaaatcggtt
      841 tctggggatc taaattcttc atcggtttca tccttggtat cgttatcggt attatgggaa
      901 ctccacatcc aattgcaggt gttgcagatg cagataaatg gcgtcttgtt atcaaaggat
      961 ggttgtctct tggtttgact gccggtgtat ctttggaact cttctcactt atcggttcat
     1021 ggttcatcgc agccgtagaa ccactatcac aaggtattac aaacgttgct actaaacgtc
     1081 ttcaaggacg taaattcaat atcggtcttg actggccatt catcgctggt cgtgctgaaa
     1141 tctgggcttg tgccaacgta cttgcaccaa tcatgttgat tgaagcagtg cttctttcaa
     1201 aagttggaaa tggtatcttg ccacttgcag gtatcatcgc tatgggtgtt actccagctc
     1261 tcttggttgt aactcgtggt aaattgctcc gtatgattat cttcggaaca ctcttgttgc
     1321 cactcttcct tctttcaggt acacttattg caccatttgc aacagaactt gctaaaggtg
     1381 taggtgcctt cccagaaggt gtgagccaaa ctcaattgat tactcactct actcttgaag
     1441 gaccaatcga aaaacttctt ggttggacaa ttggtaacac tacaactggt gatatcaaag
     1501 caatccttgg tgcagtagcc ttccttgtat tctatatcgg tatctttgct tggtacagaa
     1561 aacaaatgat caaacgtaac gaagagtacg cagcaaaagc aaaataatgc gctcctataa
     1621 aatataaact caatgaaaat caaagagcaa actaggaagc tagccgcagg ttgctcaaaa
     1681 cacagttttg aggttgtaga taagactgac gaagtcagct caaaacatgg ttttgaggtt
     1741 gtagatgaaa ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac
     1801 gaagtcagta atcatatcta cggcaaggcg acactgacga agtttgaatt ggattttcgc
     1861 agagtgtaaa ttgttgaaac taggttgtca taccatgtat accacttggg ctttggtagt
     1921 agctaactgc actaaatata atataaggag ggttttatgg ggaaaggcca ttggaatcgg
     1981 aaaagagttt atagcattcg taagtttgct gtgggagctt gctcagtaat gattgggact
     2041 tgtgcagttc tattaggagg aaatatagct ggagaatctg tagtttatgc ggatgaaaca
     2101 cttattactc atactgctga gaaacctaaa gaggaaaaaa tgatagtaga agaaaaggct
     2161 gataaagctt tggaaactaa aaatgtagtt gaaaggacag aacaaagtga acctagttca
     2221 actgaggcta ttgcatctga gaagaaagaa gatgaagccg taactccaaa agaggaaaaa
     2281 gtgtctgcta aaccggaaga aaaagctcca aggatagaat cacaagcttc aagtcaagaa
     2341 aaaccgctca aggaagatac taaagctgta acaaatgaag aagtgaatca aatgattgaa
     2401 gacaggaaag tggattttaa tcaaaattgg tactttaaac tcaatgcaaa ttctaaggaa
     2461 gccattaaac ctgatgcaga cgtatctacg tggaaaaaat tagatttacc gtatgactgg
     2521 agtatcttta acgatttcga tcatgaatct cctgcacaaa atgaaggtgg acagctcaac
     2581 ggtggggaag cttggtatcg caagactttc aaactagatg aaaaagacct caagaaaaat
     2641 gttcgcctta cttttgatgg cgtctacatg gattctcaag tttatgtcaa tggtcagtta
     2701 gtggggcatt atccaaatgg ttataaccag ttctcatacg atatcaccaa ataccttcac
     2761 aaagatggtc gtgagaatgt gattgctgtc catgcagtca acaaacagcc aagtagccgt
     2821 tggtattcag gaagtggtat ctatcgtgat gtgactttac aagtgacaga taaggtgcat
     2881 gttgagaaaa atgggacaac tattttaaca ccaaaacttg aagaacaaca acatggcaag
     2941 gttgaaactc atgtgaccag caaaatcgtc aatacggacg acaaagacca tgaacttgta
     3001 gccgaatatc aaatcgttga acgaggtggt catgctgtaa caggcttagt tcgtacagcg
     3061 agtcgtacct taaaagcaca tgaatcaaca agcctagatg cgattttaga agttgaaaga
     3121 ccaaaactct ggaccgtttt aaatgacaaa cctgccttgt acgaattgat tacgcgtgtt
     3181 taccgtgacg gtcaattggt tgatgctaag aaggatttgt ttggttaccg ttactatcac
     3241 tggactccaa atgaaggttt ctctttgaat ggtgaacgta ttaaattcca tggagtatcc
     3301 ttgcaccacg accatggggc gcttggagca gaagaaaact ataaagcaga atatcgccgt
     3361 ctcaaacaaa tgaaggagat gggagttaac tccatccgta caacccacaa ccctgctagt
     3421 gagcaaacct tgcaaatcgc agcagaacta ggtttactcg ttcaggaaga ggcttttgat
     3481 acttggtatg gtggcaagaa accttatgac tatggacgtt tttttgaaaa agatgccact
     3541 cacccagaag ctcgaaaagg tgaaaaatgg tctgattttg acctacgtac catggtcgaa
     3601 agaggcaaaa acaaccctgc tatcttcatg tggtcaattg gtaatgaaat aggtgaagct
     3661 aatggtgatg cccactcttt agcaactgtt aaacgtttgg tcaaggttat caaggatgtt
     3721 gataagactc gctatgttac catgggagca gataaattcc gtttcggtaa tggtagcgga
     3781 gggcatgaga aaattgctga tgaactcgat gctgttggat ttaactattc tgaagataat
     3841 tacaaaaccc ttagagctaa gcatcccaaa tggttgattt atggatcaga aacatcttca
     3901 gctacccgta cacgtggaag ttactatcgc cctgaacgtg aattgaaaca tagcaatgga
     3961 cctgagcgta attatgaaca gtcagattat ggaaatgatc gtgttggttg ggggaaaaca
     4021 gcaaccgctt catggacttt tgaccgtgac aacgctggct atgctggaca gtttatctgg
     4081 acaggtacgg actatattgg tgaacctaca ccatggcaca accaaaatca aactcctgtt
     4141 aagagctctt actttggtat cgtagataca gccggcattc caaaacatga cttctatctc
     4201 taccaaagtc aatgggtttc tgttaagaag aaaccgatgg tacaccttct tcctcactgg
     4261 aactgggaaa acaaagaatt agcatccaaa gtagctgact cagaaggtaa gattccagtt
     4321 cgtgcttatt cgaatgcttc tagtgtagaa ttgttcttga atggaaaatc tcttggtctt
     4381 aagactttca ataaaaaaca aaccagcgat gggcagactt accaagaagg tgcaaatgct
     4441 aatgaacttt atcttgaatg gaaagttgcc tatcaaccgg gtaccttgga agcaattgct
     4501 cgtgatgaat ctggcaagga aattgctcga gataagatta cgactgctgg taagccagcg
     4561 gcagttcgtc ttattaagga agaccatgcg attgcagcag atggaaaaga cttgacttac
     4621 atctactatg aaattgttga cagccagggg aatgtggttc caactgctaa taatctggtt
     4681 cgcttccaat tgcatggcca aggtcaactg gtcggtgtag ataacggaga acaagccagc
     4741 cgtgaacgct ataaggcgca agcagatggt tcttggattc gtaaagcatt taatggtaaa
     4801 ggtgttgcca ttgtcaaatc aactgaacaa gcagggaaat tcaccctgac tgcccactct
     4861 gatctcttga aatcgaacca agtcactgtc tttactggta aaaaagaagg acaagagaag
     4921 actgttttgg ggacagaagt agcaagagtt cgtacattaa ttggtaaaga accaaaaatg
     4981 ccgaaaacag taggttttgt atacagcgat ggtagtcgtg aaaaacttcc agtaacttgg
     5041 tctcaagtcg atgttagtca agcaggagtt gtgactgtta aaggtacagc aaatggacgt
     5101 gaggtagagg ctcatgtcga ggttctagca attgcgaaag aattaccaac agttaaacgt
     5161 attgctccaa atactgactt gaattctgta gacaaatctg tttcttatgt tttgactgat
     5221 ggaagtgtac aagagtacga agtagatagc tgggagatta cggaagtaga taaagccaaa
     5281 ctttcagtag ctggatcacg tattcaaatg actggtcagt tagctggaga aactattcat
     5341 gcaacccttg tggtagaaga aggaaatgct gcagcacctg tagtgccaac tgttactgtt
     5401 ggaggtgagg cagtaacagg tcttactagt cgacaaccaa tgcaatatcg tactctatct
     5461 tatggtgccc aactgccaga agtcacagca agtgctgaaa atgctgatgt gacagttctt
     5521 caagcaagcg cagcaaacgg catgcgtgcg agcatcttta ttcagcctaa agatggtggt
     5581 cctcttcaaa cctatgcaat tcaattcctt gaagaagcgc caaaaattgc tcacttgagt
     5641 ttgcaagtgg aaaaagctga cagtctcaaa gaagaccaaa ctgtcaaatt gtcggttcga
     5701 gctcactatc aagatggaac gcaagctgta ttaccagctg ataaagtaac cttctctaca
     5761 agtggtgaag gggaagtcgc aattcgtaaa ggaatgcttg agttgcataa gccaggagca
     5821 gtcactctca aagcggaata tgagggagct aaaggccaag ttgaactcac tatccaagcc
     5881 aatactgaga agaagattgc gcaatctatc cgtcctgtaa atgtagtcac agatttgcat
     5941 caaaaaccta ctcttccaac aacagtaacg gttgagtatg acaaaggttt ccctaaagct
     6001 cacaaagtca cttggcaagc tattccgaaa gaaaaactag actcctatca aacatttgaa
     6061 gtactaggta aagttgaagg aattgacctt gaagcgcgtg caaaagtctc tgtagaaggt
     6121 atcgtttcag ttgaagaagt cagtgtgaca actccaatcg cagaagcacc acaattacca
     6181 gaaagtgttc ggacatatga ttcaaatggt cacgtttcat cagctaaggt tgcatgggat
     6241 gcgattcgtc cagagcaata cgctaaggaa ggtgtcttta cagttaatgg tcgcttagaa
     6301 ggtactcaat taacaactaa acttcatgtt cgcgtatctg ctcaaactga gcaaggtgca
     6361 aacatttctg accaatggac cggttcagaa ttgccacttg cctttgcttc agactcaaat
     6421 ccaagcgacc cagtttcaaa tgttaatgac aagctcattt cctacaataa ccaaccagcc
     6481 aatcgttgga caaactggaa tcgtactaat ccagaagctt cagtcggtgt cctattcgga
     6541 gattcaggta ttttgagcaa acgttcagtt gataacttaa gcgttggctt ccatgaagac
     6601 catggagttg gtgcaccgaa gtcttatgtg attgagtatt atgttggtaa gactgtccca
     6661 acagctccta aaaaccctag ttttgttggt aatgaggacc atgtctttaa tgattctgcc
     6721 aactggaaac cagttactaa tctaaaagcc cctgctcaac tcaaggctgg agaaatgaac
     6781 cactttagct ttgataaagt tgaaacctat gctgttcgta ttcgcatggt gagagcagac
     6841 aacaaactag gaacgtctat cacagaggta caaatctttg cgaaacaagt tgcggcagcc
     6901 aagcaaggac aaacaagaat ccaagttgac ggtaaagact tagcaaactt caaccctgat
     6961 ttgacagact actaccttga gtctgtagat ggaaaagtta cggcagtcac agcaagtgtt
     7021 agcaacaatg gcctcgctac cgtcgttcca agcgttcgtg aaggtgagcc ggttcgtgtc
     7081 atcgcgaaag ctgaaaatgg cgacatctta ggagaatacc gtctgcactt cactaaggat
     7141 aagaacttac tttctcataa accagttgct gcggttaaac aagctcgctt gctacaagta
     7201 ggtcaagcac ttgaattgcc gactaaggtt ccagtttact tcacaggtaa agacggctac
     7261 gaaacaaaaa acctgacagt tgaatgggaa gaagttccag cggaaaatct gataaaagca
     7321 ggtcaattta ccgttcgagg ccatgtcctt ggtagtgatc ttgttgctga ggtcactgta
     7381 cgagtgacag acaaactagg cgaagctctt tcagataacc ctaactatga tgaaaacagt
     7441 aaccaggcct ttgcttcagc aaccaatgat attgacaaaa actctcatga ccgcgttgac
     7501 tatctcaatg acggagatca ttcagaaaat cgtcgttgga caaactggtc tccaacacca
     7561 tcttctaatc cagaagtatc agcgggtgtg atcttccgtg aaaatggtaa gattgtagaa
     7621 cggactgttg ctcaagccaa acttcacttc tttgcagata gtggtacgga tgcaccaact
     7681 aaactcgttt tagaacgcta tgtcggtcca gagtttgaag tgccaaccta ctattcaaac
     7741 taccaagcct acgacgcaga ccatccattc aacaatccag aaaattggga agctgtgcct
     7801 tatcgtgcgg ataaagacat cgcagctggt gatgaaatca acgtaacctt taaagctgtc
     7861 aaagccaaag ctatgagatg gcgtatggag cgtaaagcag ataagagcgg tgttgcgatg
     7921 attgagatga ccttcctggc accgagtgaa ttgcctcaag aaagcacgca atcgaagatt
     7981 cttgtagatg gaaaagaact tgctgatttc gctgaaaatc gtcaagacta tcaaattacc
     8041 tataaaggtc aacggccaaa agtctcagtt gaagaaaaca atcaagtagc ttcaactgtg
     8101 gtagatagtg gagaagatag ccttccagta cttgttcgcc tcgtttcaga aagtggaaaa
     8161 caagtcaagg aataccgtat ccagttgact aaggaaaaac cagtttctga gaagacagtt
     8221 gctgctgtac aagaagatct tccaaaactc gaatttgttg aaaaagattt ggcctacaag
     8281 acagttgaga aaaaagattc aacactgtat ctaggtgaaa ctcgtgtaga acaagaagga
     8341 aaaactggta aagaacgtat ctttacagcg attaatcctg atggaagtaa ggaagaaaaa
     8401 ctccgtgaag tggtagaagc tccgacagac cgcatcgtct tggttggaac caaaccagta
     8461 gctcaagaag ctaaaaaaac cacaagtgtc agaaaaagca gatacaaaac caattgattc
     8521 aagtgaagct agtcaaacta ataaagccca gttaccaaat acaggtagtg cggcaagcca
     8581 agcagcagta gcagcaggtt tagctcttct aggtttgagt gcaggattag tagttactaa
     8641 aggtaaaaaa gaagactaga aattttgata ccttctttat cattaaagac caaagaaaaa
     8701 ctcttgtcct aaatagaaga taaagagtct ggaacaaaat agttctcaac cacaaaaaag
     8761 ctagatattt ccaattgcgg aactctagct ttttaatttt gagtcgttct cttattgtat
     8821 agtagattga aactagagta gtacacttct acttctaaaa cattgttaga aatcgatttg
     8881 actgtcctta tcgatttgtc ctgttcttat ttcatttcac tatgttttag gaggttattg
     8941 gccataagta ccaatcccat gtcaattctc acttgacgct tgcctctcag attacatctc
     9001 ttgtaaccca aacaagcctt tatctgccca aagacaggtt ctacatcaat cttgcgttga
     9061 gcgaaaatct gtctatcttg gggagataaa agcgcctgac attctttagc tttcaagttt
     9121 tgatagcgtt cgttcatata caatcccttt tgaggggctg attcaggttc atcagcgtag
     9181 taaaccttga tttcctgttg aaagtctgtc tgtgttttct ggtacttgat atggtgaaaa
     9241 cgatagcacc agccatcagg atgtatgtag ctatcctcct tgtcattata gtgccaattc
     9301 gctaagtttt tagctgactg tttataccct ctcttttgtt ccttatcaaa catggcatat
     9361 ttaatcagaa ggggagtgat ttcttcttga aaatagactt gtatctgttc ttgaagtttg
     9421 gcggagaatt tctctgtcgt tttcttccac acaaaactat acttgttggc attggattca
     9481 atcttagttc cgtcaataaa caaacaatct aaggtcacta actcttccat ttttcaattt
     9541 ttcgaccaga gaaaatccct tgtgaatagg caaatagaag agtagataca agcattttag
     9601 ggtgataaga cgggcgacca aaggcatgat aggaggtgta gaagtgacgt tcctccaagg
     9661 tattcaccac tttttcaata gtaaagacga gatggtcttg tggtaagaag gaactgattt
     9721 ctagtggtaa agttgtttga tttgtgttat agtgaatatg catgggatag cctttctaaa
     9781 tggtttatta agcaattcta ttttaccaag actatcgtaa ccatgcaaaa aagcaacggc
     9841 aaatccgttg ccttttttgg atataagaga ctattgtccc agactctttt ctttgtataa
     9901 aattgtatag tgttattgtc tatactattt tgtagagaaa gcaaaaatcc ctagaaaatg
     9961 cttagggatt cgactatttc attaagaaca attcaacaat catctctata tacatgataa
    10021 gggttaataa aaatcctgta cgccagaaga atttgatgaa tttaggatag taaaagctgc
    10081 gtttttttaa aaggaagaaa aagacgagaa taatggctag gaacgagagg gctagcccca
    10141 gtctagggag gaaattatgg gtaaaggttt tagctgtaat caagtaatac tcaaatacca
    10201 agactggaaa agccaaatcc gcaaagtttc gtcctagttt ttttaatcta aaaagtttgg
    10261 ttaggataat gcagacgact aaggtcagta tcaataataa aatagatgct aatttcatta
    10321 aaatcatacc catattgtat cataaaaaaa ggctaatgga aacgagaaac agaggaattt
    10381 ataggaaagt caggcttttg agattgtggg tgatttttgt tataatgaaa gttataaaat
    10441 cttatgatac cttgaatgat tattatcgaa aactctttgg agaaaagact tttaaagtcc
    10501 ctattgatgc gggatttgac tgtcccaatc gtgatggaac tgtggctcat ggaggctgta
    10561 ctttttgtac ggtttccggt tctggagatg ccattgtagc cccagatccg cctatccgtg
    10621 agcaatttta taaggaaatt gactttattc atcgtaagtg gccagatgtt cagaagtatc
    10681 tggtttattt tcaaaatttt accaacaccc atgaaaaggt ggaagttatt cgagagcgtt
    10741 atgaacaggc tatcaatgag ccaggtgtgg taggaatcaa tattggaacg cgcccagact
    10801 gtttaccaga cgaaaccatc gaatatttgg ctgagttatc ggagtgcatg catgtgacgg
    10861 ttgaattggg cttgcagact acttatgaag caacctctga cctgattaac cgtgcgcatt
    10921 cttatgaatt gtaggtggaa acggtcaagc gtttgagaaa gtatccaaaa attgagattg
    10981 tttcccattt gatcaatggt ttgcctggtg aaacccatga gatgatggtt gaaaatgtcc
    11041 gccgttgtgt cacggataac gatattcaag ggattaaact gcatttgctc catcttatga
    11101 caaatactcg tatgcaacga gattaccatg agggacgttt acaattgatg agtcaggacg
    11161 aatatgtcag ggtcatctgt gaccaactgg aaattattcc caaacatatc gtcatccatc
    11221 gaattacagg agatgcgcct agggatatgc tgattggtcc tatgtggagc ctcaataaat
    11281 gggaagtcct caacagcatt gagatggaga tgagacgtcg tggaagtgtt caaggatgca
    11341 aggctgtaaa acaggagttt gaaaatgaaa agaccacttg agatggcaca tgattttttg
    11401 gctgaggtcg tgacaaaaga ggatgtcgta gtggatgcga ctatgggaaa tggtcatgac
    11461 acgctttttt tagccaagct agccaagcaa gtctatgcct ttgatattca gaagcaagcc
    11521 ttggaaaaga cccaagagcg tttgcatcag gctggcttga caaatgccca gttaatcttg
    11581 caaggccatg agacactgga ccagtttgtg acaaaagcta aggcagggat ttttaatttg
    11641 ggctatttgc cgtcagctga taagtctgtc atcacccgac cgcagacaac gattgaggca
    11701 ttagaaaagc tatgtggctt acttgtcaaa ggtggacgaa ttgctattat gatttactat
    11761 ggtcatgaag gaggcgacct cgagagagat gctgtcttgg attttgtgat ccagttgaac
    11821 caacaagagt acacagctgc catttaccga actttaaacc aagtcaacaa cccgccgttt
    11881 ttagtgatga ttgaaaaatt agagagatac agacatggat aaacaatacc tacacgaaaa
    11941 gctggatgcc atgcgccaga attttgttga atcaacctac cacgaacgag cggtgggcgt
    12001 gctagaccaa gcgcatatga gcaaaaaaat gcttaaaatc aagaaaaaat tagttgctct
    12061 tgaaatggaa cggtgccaga gaaaaattga gcacaaagac tgttccaaga ttgaccaaaa
    12121 aatcaaagag cagaaggaga tatttgaatc ctgttgtaaa aaagattaag gaggacgtgc
    12181 gtggaattac tgatttacct catcctattt ttactggtct tgattgtctc aagtacaacc
    12241 aataagctcc tacccttttt gcctctccct ttggtgcaga ttcttttggg aattgtgatt
    12301 ggtctctttt tacccaatac tgactttcac cttaatacgg agttgttttt ggcactggtt
    12361 atcggcccct tgcttttccg agaggctgaa gaagcagatg ttacggctat tttaaaacac
    12421 tggcgaatca ttgtttatct catatttcca gtgattttta tctcgaccct gagtttgggt
    12481 ggcttggccc atcttctttg gttcagcctt cccttggcag cttgcttggc tgttggggca
    12541 gcccttggtc ctacggactt ggtggccttt gcctctcttt cggagcgttt tagctttcct
    12601 aagcgcgtgt ccaatattct taagggcgaa ggactcttga atgatgcttc tggtttggtg
    12661 gcttttcagg tagctttgac agcttggaca actggagctt tttctctggg gcaagctagc
    12721 agttcactca tcttttcaat cctaggcggt tttttaattg gatttttaac agccatgacc
    12781 aaccgcttcc tccatacctt cttgctaagt gtgcgtgcaa cggatattgc cagtgaactt
    12841 ttattagaat tgagtttgcc tcttgtgacc ttctttctgg cagaagaagt tcatgtttca
    12901 ggtattattg ccgtcgtagt tgctggaatt ttaaaggcaa gtcgtttcaa gaaaatcacg
    12961 ctcctcgaag cccaagtgga tacggtgacc gagacggtct ggcatacagt gacctttatg
    13021 ctcaacggtt ctgtctttgt gattttaggg atggagctgg aaatgatagc agaacctatc
    13081 ttgaccaatc caatctataa tcctctactt ttattgctat ctcttatcgc ccttaccttt
    13141 gtcctctttg tcattcgttt tattatgatc tatggctatt atgcctatag aacccgacgc
    13201 ctcaagaaaa agctaaataa gtatatgaag gacatgtttc tcttgacctt ttcaggtgtt
    13261 aagggaacgg tgtcgattgc tacgattctc ttgataccaa gtaatctaga acaggagtat
    13321 cctctcttgc ttttccttgt tgcaggtgtg acgcttgtta gctttttaac aggtctcttg
    13381 gtcttgcctc atctttctga tgaagaggaa gaaagcaagg attatctcat gcatatcgcc
    13441 attttgaatg aagtaacgct agagttggaa aaagagttgg aagacaccag aaataaactt
    13501 tccctctatg cggctattga caattatcat ggacgtattg aaaatctcat tttaagccaa
    13561 gaaaaccagg atgatcaaga agactgggct gctttgaaac tcttgattct tagtattgaa
    13621 agtgatggtt tggaacaggc ctatgaagag gggaatatta gcaatcgtgc ttaccgagtt
    13681 taccaacgtt atctgaaaaa tatagaacaa ggaatcaatc gtaaacttgc ctcaagactg
    13741 acctattatt ttcttgtttc cttgaggatt ttacgttttc tccttcatga agtttttact
    13801 cttggaaaga ccttccgtag ctggaaggac aaggagcaaa gccgtctccg tgctcttgat
    13861 tatgaccaaa ttgcagagct ctatcttgcc aatacagaga tgattattga aagtttggaa
    13921 aacctgaagg gagtctacag acgctctttg attagtttta tgcaggagtc tcgtcttcga
    13981 gaaacagcta ttatcagcag tggtgccttt gtcgaacggg ttatcaatcg tgtcaaaccc
    14041 aacaatatcg atgaaatgct gagaggctat tatctggagc gcaagttgat tttcgaatac
    14101 gaagaaaaac gattaattac aactaagtat gccaagaaat tacgacaaaa tgtaaataac
    14161 ttagagaact attccttgaa ggaagctgcc aataccctgc cgtatgatat ggtggaattg
    14221 gtaagaagaa attagttaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt
    14281 ggattatata tgtgactgac ttcgtcagtt tcatctacaa cctcaaagca gggctttgag
    14341 caacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa gattataaat
    14401 gaaggagtat gacatgaaaa agtggtggaa agagctgata gacaagcctt tattaaaagc
    14461 ttttttgcat tattatcaag catcagatag tgagttgacc agtgtcgcag tagcctacta
    14521 ttggttgatt tcgattttcc ccctgctttt ggtggtggtc aatatcctcc cctattttca
    14581 gattcctgtg ggagaatttc tgtgctttat gaaagacgtc ctgcccccaa gcctctatga
    14641 aggtgtggaa aaaatagcca gagaagtttt gacgcaacct tcaacaggtt tattgagttt
    14701 ttctgtttta tcggcgttat ggagtttttc aaaatctatg aatttcttgc aaaaagcttt
    14761 taacaaggct tatggcgtcg aaaagagtcg tggacttatt tcccatcaga tgctaagtct
    14821 cctagtcagt tttggcttgc agctcctctt tgcgtttgcc ttgtttttga tcttatttgg
    14881 tcagatgatt ttggctttac ttgctcatta ttggacaaaa gatggcatta tctatcaggc
    14941 tttacaaggg ttggcaggtc ctctgattta cgccctcctc tttgctatct tggtcatgct
    15001 ttattatttt cttcccaata tatctaacag gaaaattagc tataccctac caggcagtgc
    15061 ctttgttctc ttagtgattt taggtttact aactctgttc tctagttatc tcaattacta
    15121 tgtccatcat ttggtggatg tccgaatttt aggttccgtt cttcttgttg ttatgatgtt
    15181 ttggtttatc ttgattgcta aaattgttat cctaggcgcg gttatcaatg ccagtatgca
    15241 gagtttgaga gatccggtct ttaaaaaaga ctaactattt atccattaca aaagcgcata
    15301 gtatcaaaac tttatagaac cttgatacta tgcgcttttg tgctttgaca gttttttgta
    15361 tagtttttgt ccaagatttc taaatcatct gattaaattc ctattttccc ctatcattgt
    15421 ccctgttttt tcgaaaatgg aaggagtata ataaatctat caataaaatg ataggaggaa
    15481 ctttatgggt catattttct tttttctaag tgtctttttg gcagggattc tatccttctt
    15541 ttctccttgt atcttacctt tgttaccggt ctatacagga gtgttactag atgataagga
    15601 tggtgctcag gcttctagcg gcaaattttc aatctcagtt actagtttat tacgaactct
    15661 ggcctttata gcaggaattt cctttatatt tattttgttg ggctatggag ctggtttttt
    15721 aggcgatttg ctttatgctt cttggttcca atatcttact ggggcaatta ttatccttct
    15781 tggtttgcac caaatggaga ttctacactt taaggggctt tataaggaaa agaggctaca
    15841 actgcaagga caggggcaaa atggtaaggg ctatagtcag gcatttttat tgggcttgac
    15901 ctttagtttt gcttggacgc cttgcgtggg gccggttctg gggtctgttt tggccttggc
    15961 ggcttcaggt ggttcaggag cttggcaggg agctggtctc atgttggtgt atacgctggg
    16021 cttggcgcta ccattcttgc ttctagctct gacctctagt tatgttttga aacatttccg
    16081 aaaacttcat ccctatctcg gaatcctcaa aaaagtgggt ggttttctca ttattgtgat
    16141 gggactcttg gttctgtttg gaaatgcttc aattttaagt caattatttg aataaaatgg
    16201 aaaggaatat caatatgaaa aaatggcaaa catgtgttct tggagcaggt tcgctccttt
    16261 gtttgacggc ttgttcaggc aagtccgtga ctagtgaaca ccaaacgaaa gatgaaatga
    16321 agacggagca gacagctagt aaaacaagcg cagctaaagg gaaagaggtg gctgattttg
    16381 aattgatggg agtagatggc aagacctacc gtttatctga ttacaagggc aagaaagtct
    16441 atctcaaatt ctgggcttct tggtgttcca tctgtctggc tagtcttcca gatacggatg
    16501 agattgctaa agaagctggt gatgactatg tggtcttgac agtagtgtca ccaggacata
    16561 agggagagca atctgaagcg gactttaaga attggtataa gggattggat tataaaaatc
    16621 tcccagtcct agttgaccca tcaggcaaac ttttggaaac ttatggtgtc cgttcttacc
    16681 caacccaagc ctttatagac aaagaaggca agctggtcaa aacacatcca ggattcatgg
    16741 aaaaagatgc aattttgcaa actttgaagg aattagccta ggaggtgtct tatgaatgat
    16801 aagttaaaaa tcttcttgtt gctaggagta ttttttctag ccataaccgg tttctatgtt
    16861 ctattgatac gaaatgcagg gcagacagat gcctcgcaaa ttgaaaaggc ggcagttagc
    16921 caaggaggaa aagcagtgaa aaaaacagaa attagtaaag acgcagactt gcacgaaatt
    16981 tatctagctg gaggttgttt ctggggagtg gaggaatatt tctcacgcgt tcccggggtg
    17041 acggatgccg tttcaggcta tgcaaatggt agaggagaaa caaccaagta cgaattaatt
    17101 aaccaaacag gtcatgcaga aaccgtccat gtcacctatg atgccaagca aatttctctc
    17161 aaggaaatcc tgcttcacta tttccgcatt atcaatccaa ccagcaaaaa taaacaagga
    17221 aatgatgtgg ggacccagta ccgtactggt gtttattaca cagatgacaa ggatttggaa
    17281 gtgattaacc aagtctttga tgaggtggct aagaaatacg atcaacctct agcagttgaa
    17341 aaggaaaact tgaagaattt tgtggtggct gaggattacc atcaagacta tctcaagaaa
    17401 aatccaaatg gctactgcca tatcaatgtt aatcaggcgg cctatcctgt cattgatgcc
    17461 agcaaatatc caaaaccaag tgatgaggaa ttgaaaaaga ccctgtcacc tgaggagtat
    17521 gcagttaccc aggaaaatca aacagaacga gctttctcaa accgttactg ggataaattt
    17581 gaatccggta tctatgtgga tatagcaact ggggaacctc tcttttcatc aaaagacaaa
    17641 tttgagtctg gttgtggctg gcctagtttt acccaaccca tcagtccaga tgttgtcacc
    17701 tacaaggaag ataagtccta caatatgacg cgtatggaag tgcggagccg agtaggagat
    17761 tctcaccttg ggcatgtctt tacggatggt ccacaggaca agggcggctt acgttactgt
    17821 atcaatagcc tctctatccg ctttattccc aaagaccaaa tggaagaaaa aggctacgct
    17881 tatttactag attatgttga ttaatactct tcgaaaatct cttcaaacca cgtcagcgtc
    17941 gccttaccgt atgtagggtt
//
LOCUS       DAVKGU010000034        17893 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKGU010000034 DAVKGU010000000
VERSION     DAVKGU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.034"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VW077_001104"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VW077_001104"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9455129.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHHHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VW077_001105"
     CDS             1377..1886
                     /locus_tag="VW077_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455130.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4368
                     /locus_tag="VW077_001106"
     CDS             1963..4368
                     /locus_tag="VW077_001106"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9455131.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIEFSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAE
                     GAGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     AHKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4436..5437)
                     /locus_tag="VW077_001107"
     CDS             complement(4436..5437)
                     /locus_tag="VW077_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9455132.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSNHRCIFTEITF"
     gene            5593..6039
                     /locus_tag="VW077_001108"
                     /pseudo
     CDS             5593..6039
                     /locus_tag="VW077_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6165..6431
                     /locus_tag="VW077_001109"
     CDS             6165..6431
                     /locus_tag="VW077_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9455133.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            6692..8941
                     /gene="metE"
                     /locus_tag="VW077_001110"
     CDS             6692..8941
                     /gene="metE"
                     /locus_tag="VW077_001110"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9455134.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNSITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9005..9871
                     /gene="metF"
                     /locus_tag="VW077_001111"
     CDS             9005..9871
                     /gene="metF"
                     /locus_tag="VW077_001111"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9455135.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     FPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10556..12769
                     /gene="pnp"
                     /locus_tag="VW077_001112"
     CDS             10556..12769
                     /gene="pnp"
                     /locus_tag="VW077_001112"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9455136.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12785..13402
                     /gene="cysE"
                     /locus_tag="VW077_001113"
     CDS             12785..13402
                     /gene="cysE"
                     /locus_tag="VW077_001113"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9455137.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13414..14298
                     /locus_tag="VW077_001114"
     CDS             13414..14298
                     /locus_tag="VW077_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455138.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14385..15728
                     /locus_tag="VW077_001115"
     CDS             14385..15728
                     /locus_tag="VW077_001115"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9455139.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15721..16107
                     /locus_tag="VW077_001116"
     CDS             15721..16107
                     /locus_tag="VW077_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9455140.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16111..16995
                     /locus_tag="VW077_001117"
     CDS             16111..16995
                     /locus_tag="VW077_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9455141.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGSQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17132..17731
                     /locus_tag="VW077_001118"
     CDS             17132..17731
                     /locus_tag="VW077_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455142.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNLSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNTK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
BASE COUNT         5386 a         3445 c         4041 g         5021 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagta gtaattgaat aggaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctggc tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga ctgctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccatcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta ctttccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaaat gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggggccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggct ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggactagg taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gctaggtgtt tgggagcata atacaaaagc tcaagcgttt tataaccgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag agaattcttc tattgaaatg ataggaaagg
     1921 aaaagaacca gtgtggcaat ggtcattctg gagaactaaa ttatgcttgt atcttataaa
     1981 tggttaaaag aattggtggg cattgatgtg ccatcacagg agttggctga aaaaatgtca
     2041 accacaggga tcgaggtcga aggtgtcgaa tcaccagctg ccggtctttc aaaaattgtc
     2101 gtcggtgaga tcttgtcctg cgaagatgtg ccagaaactc accttcatgt ttgtcaggtt
     2161 aacgttggcg aagaagaaga gcgtcagatc gtttgtggtg ccccaaatgt gcgtgcaggc
     2221 atcaaggtta tggtggctct tccgggagct cgtatcgctg acaattacaa aatcaaaaaa
     2281 ggaaaaatcc gtggcttaga gtcacttgga atgatctgtt cacttggtga attgggtatt
     2341 tctgactcag ttgtacctaa gaaattcgca gatggtattc aaattttgcc agaaaatgcc
     2401 gttccaggtg aggaagtctt ttcatatcta gacttggatg atgaaatcat cgaattttct
     2461 atcacaccaa accgtgcaga tgccctttct atgcgtggag tggctcacga agtggcagcc
     2521 atatatgaca aggcagtcaa ctttaaagaa tttactttga cagaaacaaa tgaagctgcg
     2581 acagatgccc tttctgtggg cattgagaca gacaaggcgc cttactatgc agctcgtatc
     2641 ttggacaatg tgactatcgc accaagtcca caatggttgc aaaaccttct catgaacgaa
     2701 ggcatccgtc ccatcaataa cgttgtagac gtgacaaact acatcctgct ctactttggt
     2761 caacctatgc atgcttttga cttggacaca tttgaaggga ctgacatccg tgtgcgtgaa
     2821 gcgcgtgctg gtgaaaaatt ggtgaccttg gacggtgaag aacgtgactt ggacgtgaat
     2881 gacctagtca tcactgttgc agacaagcca gtagcccttg caggtgtcat gggtggtcaa
     2941 gcaacagaaa tctctgaaaa atctagtcat gttgtccttg aagctgctgt tttcaatggc
     3001 aaatctatcc gtaagacaag tggtcgcctg aaccttcgtt ctgagtcatc ttctcgcttt
     3061 gaaaaaggaa ttaatgtggc aacagttaat gaagcccttg atgcggcagc tagcctgatt
     3121 gcggaacttg caggtgcgac ggtgcgtaag ggtatcgttt cagcgggtga gcttgatact
     3181 tcagatgtag aagtttcttc aactcttgct gatgttaacc gtgtcctcgg aactgagctg
     3241 tcttatgctg atgtcgaaga cgtcttccgt cgtcttggct ttggtctttc tggaaatgca
     3301 gacagcttta cagtcagagt cccacgtcgt cgttgggata tcacaatcga agctgacctc
     3361 tttgaagaaa ttgctcgtat ctatggttat gaccgcttgc caactagtct accaaaagac
     3421 gatggtacag caggtgaatt gacagccaca caaaaactcc gccgtcaagt tcgtactatt
     3481 gctgaaggag caggtttgac agaaatcatc acctatactc taacaactcc tgaaaaagca
     3541 gttgagttta cggctcaacc aagtaacctt acggaactca tgtggccaat gactgtggat
     3601 cgttcagtcc tccgtcaaaa tatgatttca ggtatccttg ataccgttgc ctacaacgtg
     3661 gctcataaga ataaaaactt ggccctttac gagattggaa aagtctttga acaaacaggt
     3721 aatccaaaag aagaacttcc aaatgaaatc aacagttttg cctttgcctt gacaggcttg
     3781 gttgctgaaa aagatttcca aacagcagca gttccagttg atttcttcta tgctaaggga
     3841 atccttgaag ccctatttac tcgtttggga ctccaagtaa cctatacagc aacatctgaa
     3901 atcgctagcc ttcatccagg tcgtacagcc gtgatttcat tcggtgacca agttcttggt
     3961 ttccttggcc aagtgcatcc agtcactgcc aaggcttacg atattccaga aacgtatgtg
     4021 gctgagctta acctttcagc tatcgaagct gcgcttcagc cagcgactcc atttgtagaa
     4081 atcaccaaat tcccggcagt cagccgtgac gttgcccttc tcctcaaggc agaagtgact
     4141 catcaagaag ttgtagatgc tatccaagct gccggcgtga aacgtttgac agatatcaaa
     4201 ctctttgacg tcttctcagg tgagaaattg ggacttggta tgaagtcaat ggcttatagc
     4261 ttgaccttcc aaaatccaga agatagctta acggacgaag aagtcgcacg ctatatggaa
     4321 aaaatccaag catcgctcga agaaaaagtc aatgcagaag tgcgttaaac gatagtatga
     4381 tacaaattcc ccctctagtt tatgaacact agaggggtga ttttatataa aataattaaa
     4441 atgtgatttc tgtaaaaata catctatgat tagagttttg aaaacttaca atgtctaaat
     4501 ctttaacata gtagtggttt ttaggcaata aaatatgatc tattgttgca ttaaaaagtt
     4561 ttggactttg gctattccaa gttcctcttt caaaaggtgg cagtacattt aatgcgtccc
     4621 tatgagagct tatttttgca agtgctccat gacgcatagt tgcattaaaa tcacctgcaa
     4681 taatagcctt tggatatttt gaagccaatt gattatgaat gatgtttaag tcttgcttcc
     4741 agatttccat taaacctggc agaggaggag cagtatgcaa ggcaatgata tctggtatat
     4801 tttgttttct cgaatgtaat acaattgtcc cgaaccgtgt tgtatgaaaa gttttagctt
     4861 ctgtatagaa accataactt ttcttgacaa tcacagtcac aggagctatt ccactattgg
     4921 taggtggaga agtgaaaata tcatagttgg ccatagaaag tccaacttga tgaaacaata
     4981 gtttgattct ctggttttct tgctcacctc tgatattggt agctagttca gggaatatag
     5041 ccatatcggc gtcaaaatgg ctaaaaattc gctcaatatg ttgtgcttct atttgattag
     5101 cgacgttcca agttactaat tttaaggttt tagtcttatt tgatgcagaa gaaagggttt
     5161 tatctgttcc gaataatagt aatatgagcg atagtaagag caaaatgcca gagtaaaacc
     5221 gtatttgttt atttttcttg taaaaagata gggaaaaaaa tacgaaactt aaaatagcag
     5281 ccatagttgc agtagctaag gggaaggcat aaatctgtgc caagataggt tgggtagata
     5341 gccaaatgtt aagattagaa gatgggaaaa aagaataagt tataataggg atagaaaaga
     5401 gaatgaccga aagggcagag agaaatgttt taatcattgt gtgagtacct caatttgcaa
     5461 tggttgctcg gagctaatgt gactttgtga ctttacttga ttgatattct ttttcttgtc
     5521 ttcctcatag attaagtaca acccaagtaa aaaaagtcca atttaagatt gaaatgatta
     5581 aaaggcacag aaatgcaaga caattatact acaaaaggca aacatttgac aatcgatagc
     5641 cgtcgcttaa tcgaacgatg gaaaaaagaa ggaaaatcaa atagagaaat tgcctctcta
     5701 cttggaaaag ctcctcaaac tatccatact gaaatcaagt atgggacagt ccgacaatgt
     5761 cttggaaaag ggcgcttcaa agagatttat tctgccgact acgctcaaca gtcttatgaa
     5821 aataatcgca agcactcggt caagaaatca agcttgacta agaaactaaa ggaaaagatt
     5881 ctccactatc ataaccaaaa attttcgcct gaaatgatgg tgatggctaa aggggttaac
     5941 gtgggaattt caaccattta ctattggatt catcgtggaa aattggggtt aagcaagcag
     6001 gatctgcttt atcctagaaa aggaaaagcg cttaagaaat aggctagcac caactttaaa
     6061 cccgctggtc aatctatcga atcataattc gctcattttt ttaaaaatac atagacgtaa
     6121 aaaaaagagg gttaagtgat gaagaatagt gctattggga gtaattggaa ggatgtccat
     6181 ctgagctctt tagcaaggag gaaatccttg aaagtgatat gcgagtggct atcatgagcg
     6241 agttgattga ggctaggtat gaacaaggaa tcagtcagaa aaagctggaa gaagtcagtg
     6301 gaataagcca gcctgtcata gctaggatgg agacaggaga gagcagtctt cagttggata
     6361 cggtcttaaa agttctaacc agtctaggaa agacactagc agtcgttcca cttgaacagg
     6421 ggaaaagttg actagaatca gttatagtct tttctaataa caggtcatag tcacttataa
     6481 gaattactaa taacatgttt tagtattcta attggaatat agctgtaaag taagaattac
     6541 aaaggatttg agacgtttgc ttcaagtctt tttcttttgt ccgaaacaga tatatagttt
     6601 caaactatat caaaccctaa ttttttacaa ttatacagac gctttctttt ctgttaagat
     6661 agtttcaaca acaaattttg gaggacacat catgtcaact acaatcatcg gtttccctcg
     6721 tttgggcgaa ttccgtgaat taaaatttac aactgaaaaa tactttagaa aagaaatctc
     6781 agaagaagaa cttttagcag cagcaaaaga cttgcgtgct aaacactgga acattgtcaa
     6841 agaaaaaggc atcactgaaa ttccatcaaa tgacttttct cactatgata acttcctaga
     6901 tgcggctttc cttttcaacg tggtgcctgc atccgttcaa aacttggact tgtctgacct
     6961 tgagcgctac ttcgctttgg gacgtggtta ccagggagaa aaaggggacg ttcgcgccct
     7021 tccaatgaag aaatggttca acactaacta ccactacatc gttcctaaat ttgaaaaaga
     7081 cactcaagta aaattggctg gtcacaagat tttcgatgag ttccaagaag caaaagaact
     7141 tggattgaac actcgtcctg ttcttgtagg tccattcact ttccttcaat tgtcagactt
     7201 tgaagaaggc gtgaaagcag acgacttcgt agatagctta gtggttgctt accaagaagt
     7261 ttttgctaaa ttggctgaac ttggtgcgac tcgcatccaa ttggatgaag cggctcttgt
     7321 aaaagacttg acagctgaag aaaaagctct cttcttgaac ctttacaaca aactcttggc
     7381 tgacaaaaaa ggtcttgaag tcttgcttca aacttatttc ggtgatgttc gtgacgtata
     7441 cgctgacctt gtaaacttgc cagtagatgc tatcggtctt gactttgttg aaggtaagaa
     7501 aactcttgaa ctcgttaaag gtggcttccc agctgacaag actctctatg taggtattgt
     7561 caatggtaaa aacatctggc gcaacaacta cgaaaagagc ttggctgttc ttgagcaaat
     7621 cccagctgaa aacatcgttt tgacaagctc ttgctcactt cttcatgtgc catttacaac
     7681 tgctaatgaa gaatttgaac cagcactctt gaaccacttt gcctttgcag ttgaaaaatt
     7741 ggatgaaatc cgtgacttgg atgctatccg caatggtcaa ggttcagaag ctcttgcagc
     7801 caacaaagaa ctctttgcga ctgagcgtgt tggtgaaaat gctgaacttc gtgcgcgtat
     7861 cgctggcttg acagacgcag actacactcg tttgccagcc tttgcagaac gtgaagctat
     7921 ccaagaagaa gctttcaaac ttccagctct tccaacaaca acgattggtt cattccctca
     7981 aacaaaagaa gttcgtgcca aacgtttggc ttaccgtaaa ggtgaattgt ctcaaaaaga
     8041 gtacgatgct ttccttgctg aaacgattga cgaatggatc aagtggcaag aagatattga
     8101 ctttgatgtc cttgttcacg gtgaatttga gcgtaatgac atggttgagt acttcggtca
     8161 aaacttgtca ggatacctct tctctaaaaa tggatgggta caatcatacg gtatgcgtgg
     8221 ggtaaaacca ccaatcatct ggggtgatgt cactcgtctt aactctatca ctgttaaatg
     8281 gtctagctat gcacaaagtc gtacaaacaa acctgttaaa ggtatgttga ctggacctgt
     8341 taccatcctt aactggtcat tcccacgtga agacatctct atcaaggatt caactcttca
     8401 aatcgccctt gctatcaagg atgaagtact tgaccttgaa gctgctggtg tgaaaatcat
     8461 ccaaatcgac gaggctgctc ttcgtgaaaa attgccactc cgccgtagcg actggtacga
     8521 agactacctt gactgggcta ttcctgcctt ccgcttggta cactcaacag tagcgccaga
     8581 cacacaaatc cacactcaca tgtgttactc agaatttaca gatatcatcc cagctatcga
     8641 taacatggat gcagacgtta tttcctttga agctagccgt tcaaaccttg aaatcttgga
     8701 cgaactcaaa gcgaaaaact tccaaacaga agtgggacct ggggtttacg atatccactc
     8761 acctcgtgta ccaaatgaag gcgaaatcga caacacaatc gaagccatcc ttgctaaagt
     8821 gccaagcaag aaagtttgga tcaaccctga ctgtggtttg aaaacacgtg gtatcccaga
     8881 aacaaaagaa agcttgatcc gccttgttga agcagctaaa gctgcgcgtg agaaattgta
     8941 agattggatt tctctccata cagtggttgt tcaaaaagaa atcaactaga aaaggttatt
     9001 acatatgtca cgccaaacac cgtcactctc atttgaagtg ttccctccaa acccagccgt
     9061 gggtaatggt aacattattt cagccttgca ggatatgcag gagttggctc cccattttat
     9121 cagtgtaact gccagcaata ataaatttaa tatcaaggaa acgacggttc gtttggctga
     9181 ctttatccaa aatgacttgg cgattccgac cattgcccac ttgccagcta tttatctaac
     9241 taaggacaag gttgctgaaa ctattgctga cttggacaaa gttggggtgc agaaaatctt
     9301 ggctcttcgt ggggatatta ttccagacgt agaaccacaa aaggatttcc gctatgcaac
     9361 cgacttgatt gagtttatca aggaacaagc ccctcacttt gatattattg gagcttgcta
     9421 tccagaaggg catccagatt cgccaaatca gatttcagat attcaaaatc ttaagaagaa
     9481 agtggatgca ggctgttcga gcctcgtaac tcagctcttc tttgataatg agcgcttcta
     9541 tgattttcag gacaagtgta ttttggctgg gattgatgtt cccattcatg caggaattat
     9601 gccaattctg aatcgaaatc aggctctccg actcttgaag acttgtgaga atatccattt
     9661 tccacgcaaa tttaaagcca tcttagacaa gtatgagcat gaccctgagt cgctcagagc
     9721 agcaggactt gcctatgcag tggaccaaat cgtggacttg gtaactcagg atgttgccgg
     9781 tgtgcatctc tatacgatga ataatgctga tacagcaaaa tacatccatc aagcaaccca
     9841 tgccttgttt aatcaccagt ctctaggata ataaaagcaa accattcttc tcaggtgagg
     9901 ggaatggttc ctttttaatg gtaaagacct cactttttaa gaaaaatatg ataaaataaa
     9961 ttctgtacgt acttgataca aagatgaagg tattaaataa tacaatgcat ttaaactgta
    10021 agatttaacg acacgagtga gtcaaatcga tgttatttga cgagagagtt agtaaagcat
    10081 ttagctaatc gcccgatagc gattagcgtg aaagctttag atactttagt attaaacctt
    10141 tctaatgatg tatcttgaaa actgaacttg cctacaactc tgtggaaaaa gataaatctc
    10201 cctaggagca gtcgctccgt cgttcgattt cctattttcc tttgagttgc ttgacggctt
    10261 ggtatcttga tgacttgaat tttggtctca tttcttagga aaaaagataa gcttcctagc
    10321 actcgtcgag tgcgacgtca ccttcctatt tttctacaga aatgttcggc aagccgaacc
    10381 gtccaaaata tcttgtgtaa ttgaacacgg ccgaaaagct gtgtaaaaaa gataaactgt
    10441 cttgtcttca tcgaagactt cgtcagtttc ctatttttac tttgcttttg acgtccttgg
    10501 tatcttgatc tttgtaggca aggcgtataa tttcatcaat ccaaagggga ttaaaatggc
    10561 aaaacaagtg tttcaaacga cttttgcggg tcgtgagtta attgtagaaa ctggtcaggt
    10621 tgctaagcaa gcaaatggct ctgttgtcgt acgttacggt gagtcaactg tcttgactgc
    10681 tgccgttatg tctaagaaaa tggcaactgg ggatttcttc ccactccaag tcaactacga
    10741 agaaaaaatg tatgcggctg ggaagtttcc tggtggcttt atgaaacgtg aaggacgtcc
    10801 ttcaacagat gcgaccttga cagcgcgttt gattgaccgt ccgattcgtc ctatgtttgc
    10861 ggaaggtttc cgtaatgaag tccaagtcat caatacagtg ctttcttatg atgaaaatgc
    10921 atctgcacca atggctgcta tgtttggttc atctttggca ctgtctattt cagatattcc
    10981 atttgacgga ccaattgctg gggtacaagt gggatatgta gatggccaaa tcatcatcaa
    11041 cccaagtcaa gaacaagcag agcaatctct tcttgaattg acagtagctg gaaccaagca
    11101 cgctatcaac atggtagagt ctggtgccaa agaattgtca gaagaaatca tgttggaagc
    11161 gcttcttaaa gggcacgaag ctgtcaaaga attgattgcc ttccaagaag aaatcgttgc
    11221 tgctgtcggt aaagaaaaag cagaagtgga attgcttcac gtggatgctg aattgcaagc
    11281 tgaaatcatt gcagcctaca acagtgacct ccaaaaggca gttcaagtag aagagaaatt
    11341 ggcccgtgaa gctgcaactc aagcagtgaa agaccaagtg actgccgttt acgaagaaaa
    11401 atatgcgaac cacgaagaat ttgaccgtat tatgcgtgat gtggctgaaa tcttggaaca
    11461 aatggaacac gcagaagtgc gacgtttaat tacagaagac aaggtgcgtc ctgatggtcg
    11521 taaggtcgat gaaatccgtc ctttggatgc ggttgttgac ttccttcctc gtgtacatgg
    11581 ttcaggtctc tttactcgtg ggcaaactca agctctttca gtcttgacct tggctccgat
    11641 gggagaaact caaatcattg atggtttgga tccagagtac aagaaacgct ttatgcacca
    11701 ctataacttc cctcaatatt ctgtagggga aacaggtcgt tacggtgcgc caggtcgtcg
    11761 tgaaatcggt cacggtgccc ttggtgagcg tgctcttgct caagtcttgc caagcttgga
    11821 agaattccca tacgctatcc gtctagtagc agaagttttg gaatcaaacg gttcttcatc
    11881 tcaagcttct atctgtgcgg gaactcttgc ccttatgact ggtggtgtgc caatcaaggc
    11941 gccagtagct ggtattgcta tgggacttat ctcagatgga aataactaca cagtattgac
    12001 agatatccaa ggtttggaag atcactttgg agatatggac ttcaaggttg caggtactcg
    12061 tgatgggatt acagcccttc aaatggatat caagattcaa gggattactg cagaaatctt
    12121 gacggaggct cttgctcaag ccaagaaagc gcgttttgaa atccttgatg tcattgaagc
    12181 aaccattcca gaagttcgtc cagaattggc tccaactgct ccgaaaattg atacgatcaa
    12241 gattgatgtg gacaagatta agattgtcat cggtaagggt ggagaaacca tcgacaagat
    12301 tatcgctgaa acaggtgtta agattgatat agacgaagaa ggaaatgtgt ctatctactc
    12361 tagtgaccaa gatgctatta accgtgccaa agaaattatt gctggtttgg ttcgtgaagc
    12421 caaagtggat gaagtttacc gtgctaaagt cgttcgtatc gagaaatttg gtgcctttgt
    12481 taacctcttt gataagacag atgcccttgt tcatatctct gagatggctt ggactcgtac
    12541 caatcgtgta gaggatttgg tagaaatcgg ggatgaagtt gatgttaagg ttatcaagat
    12601 tgatgaaaaa ggccgtatcg atgcctctat gaaggctctt ctacctcgtc cgccaaaacc
    12661 tgagcatgat gaaaaaggtg aaaagtctga gcgccctcac cgcccacgtc atcacaagga
    12721 tcacaaacct aagaaagaat ttacagaaac accaaaagat tcagagtaag aaaaggagaa
    12781 atgtatgggg tggtggcacg aaaccattga tatcgtaaaa gaaaatgatc cagcggcccg
    12841 caccactttg gaggttttgt tgacttatcc aggtgtcaag gccttggcgg cccaccgtct
    12901 ctcgcatttt ctctggaagt acgacttcaa actcttagct cgtatgcaca gtcagttttg
    12961 gcgcttttgg actcagattg agattcatcc aggagcccag attgattcag gtgtctttat
    13021 tgaccatggt tctggtctgg tgattggaga gacagcgatt gttgaaaaag gcgttcttct
    13081 ctatcacgga gtgactctcg ggggaacagg gaaagactgt ggcaaacgcc atccgactgt
    13141 acgaaaggga gccctcatat cagcccatgc ccaagttatc gggcctgtgg aaattggtga
    13201 aaatgccaag gtcggtgctg cagcagttgt cgtagcagat gtacctagtg atgtgacggt
    13261 tgtcggtatt ccggccaaga ttgtccgtct tcatggtaag aaagatgagc ctgttattca
    13321 cgaagtcgaa gaaaaacggg agtattatgt caataaactc gagcaggcta aagatgccag
    13381 tcacagatcg tctggtttgt agaggatacc tatatgattc aagcaagaaa caagttaagc
    13441 caagaggagc tatctgaggc gaaaaaagta attaactgtt gccaaaacta tgacggtacc
    13501 tatcgcgatc cctatctctc taacatgctt aattttgacc caaacatgcc cgcctttttc
    13561 ctttattatg aaaaaggcaa acttgttggt ttattaactg tctatgcaga tgaccaagat
    13621 gtagaagtga cgatattggt tcatccaggt catcgccgtc aggggattgc gcgtgcattg
    13681 tttactagtt ttgagagaga aacagcttct ttccccattc ggtcagtcac ttttcagaca
    13741 gaacgtattt ttttagagaa tcatcctgac tttgccagta actggggact aatcgaggat
    13801 gaagagacag aaacttggtt aggtaaggat agaagacctt atccgttagc aaatgtttcc
    13861 catcttgaag ttttgttagc agatagttcg tatcaggatc aaattagtca attaaaattt
    13921 caggcatttt cagaggaaca tgaatcgaga gaagttgtgg atagatatgt cgctgaagct
    13981 ctgaaggatc cagaaagtcg cttatatatt ttgttaaaag acggtcaggt tattgggact
    14041 tgcacggtag atttatcgac taatacgaat tacttctacg gtttagcaat atcggaacct
    14101 gaacgtggga aaggctatgg aagctactta gcaaaatccc tcgtcaacca actaattgag
    14161 caaaatgaca aggaatttca gattgcagtg gaagatagca atgtaggtgc caaacgtttg
    14221 tatgaaaaaa ttggctttgt caaacagaca caggtggttt atctgaatga gaaaggagca
    14281 agggattccg aagtgtagag acattcggac tgaaatttac ttgagctctt agtttagtga
    14341 tgaaactaac agttcttgga ttttagcttt cctgattatg atttatgatt aaaatctatg
    14401 acaccatgtc tcgtgatttg cgagaatttg tcccgattga ggacggcaag atcaagatgt
    14461 atgtttgtgg gccaaccgtg tacaactata ttcacgtggg aaacgcccgt tcgacggtag
    14521 cttttgatac gattcgtcgc tattttgagt accgtgggta taaggttgcc tatatttcca
    14581 attttacaga tgtggatgat aagattatca accgtgccag ggaagaaggc atcacacctc
    14641 aggaggttgc ggataagtac atcgctgcct ttcgtgagga tgtgacggcc ttaggcgtga
    14701 aacctgcgac tcgccatccg cgtgtagtgg agtttatggc agacatcatc cgttttgtgg
    14761 aagacttgat cgagaaaggc tttgcctatg agagtcaagg ggatgtctat ttccgtgtag
    14821 aaaaatccca caactatgct aaattggcta ataaaacctt ggaagatttg gagctaggtg
    14881 cttcaggtcg taccgatgaa gaaacggctc gtaaggaaaa tcctgtagac tttgctctat
    14941 ggaaatcttc caaaccaggt gagatttctt gggatagtcc ttggggacct ggacgtccgg
    15001 gttggcatat tgagtgttca gtcatgtcga cagagatttt aggtgatacc attgatatcc
    15061 acggtggtgg agctgaccta gagtttccac accacaccaa tgaaattgcc caatctgaag
    15121 ctaaaacagg caaggctttt gctaactact ggatgcacaa tggctttgtc aatatcgaca
    15181 atgtcaaaat gtctaagtcc ttgggtaact tcattacggt acacgatgcc cttaaaactc
    15241 ttgatgggca agtacttcgt ttcttctttg cgacccagca ctaccgtaag cctatcaact
    15301 tcacggaaaa ggcagttcgc gatgccgaga ccaatctcaa gtatctgaag aatacttacg
    15361 aacaaccatt tacaggaaac gtggatgccc aagagttaca aaactttaaa gataagtttg
    15421 tagcagctat ggatgaagat tttaatgctg ccaacggtat cacagttgtc tttgaaatgg
    15481 ccaaatggat caactcaggg aactatgatg caagtgtcaa gcaagctctt gcagatatgt
    15541 tagaaatttt tggaattgtc tttgttgagg aagttttgga tgcagagatt gaagacttga
    15601 ttcaaaaacg ccaagaggcg cgtgccaatc gtgactttga gacagcagac caaatccgtg
    15661 accaattggt tactcaagga attaagctcc ttgataccaa ggatggagtg aggtggacac
    15721 gtgattgatg tcaatctcat taacgggatt gcgctagcct ttgagggaga tgcggtgtat
    15781 tctatgtata ttcgccgcca cctcatcctc aaaggtatga ccaagcccaa taaactccac
    15841 caagaggcga ccaagtacgt gtcagccaag gctcaggctc gtctgattgc tctcatgttg
    15901 gaggagcaga tcctaacgga aaaagaagaa gaaatctaca aacgtggccg caataccaat
    15961 agtcacacaa aggctaaaaa tgcagatgtc gtgacttatc gtatgtccac gggatttgaa
    16021 gcagttatgg gctatctcca tatgactgag aatctggaac gtcttgagag tttggtttca
    16081 tggtgcatcc aaaaagtgga gggctagaac atgagggcaa aagaactaca agactggttt
    16141 cctgaggctc ggatttcaga ccaaccagta gagaaagagg gctatctcac gcttccttta
    16201 gcttctcagc agtggatttt gctggaggaa gctgggctca gcgagcgtga aaagcagttg
    16261 gttgcccttt tgacccagca ggagcaggct cgttcgctaa acccttggta ttcctatctg
    16321 gttgaaggca agggacaggc accgcaagtt tttaaaaaga ttcagttggt ttattgccat
    16381 ctttcttatt ttcagcagga aaatctggct tcttggctag atatgatgcg gactcttttt
    16441 ccgaattgtc agacagtgct acaggtcgga tctcaggatt atgttttcgt gcttcaacaa
    16501 gacaaataca cttctgtaag agatatttta agtgatacga ttgaagcggt tgagtatgac
    16561 tttggacttc gtctttctat catgttgggt caggtttggt ctcagacagg acatcaagcc
    16621 ctatcagact taatcaaagc tgagcgggat ttgttcaaga catggtggcg tcagggtcac
    16681 caaggtgttc atactttttc tcagctctat ctttggagta tgggagaaag actcgtggac
    16741 ttgaagccaa ttaaagaatg tctacaccag atgattttgg atcaagatca gattcaggaa
    16801 atcattctct ctctttggga aaatagtgct gttctcacta aaacagccca gcaactctat
    16861 ctgcaccgca attctctcca atacaagatt gataaatggg aagagttgac agggcttcag
    16921 ttgaaagagt tgaccgacct gaccttgtgt tatcaattga ttttaggttc tttgtcaact
    16981 atagttggtt tgtaaagaag ttaatatttg gagaagagga tggccatctt ctccattttt
    17041 atgtgcagag ttatagtgga ttgatgctgg gaaagtacac tgtgactgct aaaacattcc
    17101 tagaagttgc tttgatttcc ctaatctatt tatgcaaatt ttatgttatt ttactataac
    17161 agttgttgct aagccaaata atagtgggga agttcattta gacgtaagca ttgaagataa
    17221 tcagggaggt agtgggcata atttcagttc tgtttcaagt agctcacaaa cagctaaata
    17281 tgaagaaact ggttataata acaatttatc attatatata acgattgata aaacgtctga
    17341 tgcaacagct cttttgaaat taaagttgaa taatgttgat aatcaacctg ctactgaagt
    17401 tcctagttca ggaattactg taaaattaaa tactaaagat aatgctggaa actggacaag
    17461 tgcttcgaat aaaaaagaaa taacagtaaa aattgtttct gctaaaccga catatccaga
    17521 caaaatctta gtgaaaaatc ctgataatat aaaagataca gaaaaaaatg ccattattga
    17581 aaaattgaaa gaggcaaata aaaatcatcc agcaggagct ccaacctttg ctaaaggtga
    17641 aggagagcat gcaaatgata ttgtagcaac ttattcagat ggtacaactt attatgtacc
    17701 gttaaatgat gtgacaaaat atgcgaggta gtggctgtac cactcactta ttcacctcct
    17761 cgtgatttgt agtagtgata ggctttctca ctattattat aaaacaaaat aaagatcaca
    17821 acactttttc attctgtgtt gtgccttgag tgaaacgaaa ggaatgaatt ataaatatga
    17881 aaagtatagt cac
//
LOCUS       DAVKGU010000035        17526 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKGU010000035 DAVKGU010000000
VERSION     DAVKGU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.035"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(1..198)
                     /gene="blpI"
                     /locus_tag="VW077_001119"
     CDS             complement(1..198)
                     /gene="blpI"
                     /locus_tag="VW077_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEU9455143.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            480..1067
                     /locus_tag="VW077_001120"
     CDS             480..1067
                     /locus_tag="VW077_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9455144.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLR"
     gene            994..2637
                     /locus_tag="VW077_001121"
     CDS             994..2637
                     /locus_tag="VW077_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9455145.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILKACEVAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2648..4009
                     /locus_tag="VW077_001122"
     CDS             2648..4009
                     /locus_tag="VW077_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9455146.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVISSIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTSNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGENRI
                     PTGTEIAQVFPIITDTREVLITYYVASDYLPLLDKGQTVRLKLEKIGNHGITIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            4066..4221
                     /gene="blpC"
                     /locus_tag="VW077_001123"
     CDS             4066..4221
                     /gene="blpC"
                     /locus_tag="VW077_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9455147.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGWWEELLHETILSKFKITKA
                     LELPIQL"
     gene            complement(4263..5603)
                     /locus_tag="VW077_001124"
     CDS             complement(4263..5603)
                     /locus_tag="VW077_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9455148.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLAQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKAFKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5617..6354)
                     /locus_tag="VW077_001125"
     CDS             complement(5617..6354)
                     /locus_tag="VW077_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9455149.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(6359..6697)
                     /locus_tag="VW077_001126"
     CDS             complement(6359..6697)
                     /locus_tag="VW077_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9455150.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEKASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6974..7303
                     /locus_tag="VW077_001127"
     CDS             6974..7303
                     /locus_tag="VW077_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9455151.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7471..8520)
                     /locus_tag="VW077_001128"
     CDS             complement(7471..8520)
                     /locus_tag="VW077_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9455152.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8517..9248)
                     /locus_tag="VW077_001129"
     CDS             complement(8517..9248)
                     /locus_tag="VW077_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455153.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMLRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9316..9726
                     /locus_tag="VW077_001130"
     CDS             9316..9726
                     /locus_tag="VW077_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9455154.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQIAETIKNV"
     gene            9736..10023
                     /locus_tag="VW077_001131"
     CDS             9736..10023
                     /locus_tag="VW077_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455155.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            10185..10424
                     /locus_tag="VW077_001132"
     CDS             10185..10424
                     /locus_tag="VW077_001132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455156.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHGKPTLTWFEEIFEEYYSPFHN"
     gene            complement(10758..11894)
                     /gene="dnaJ"
                     /locus_tag="VW077_001133"
     CDS             complement(10758..11894)
                     /gene="dnaJ"
                     /locus_tag="VW077_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9455157.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRGKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12296..12652)
                     /locus_tag="VW077_001134"
     CDS             complement(12296..12652)
                     /locus_tag="VW077_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9455158.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12654..14477)
                     /gene="dnaK"
                     /locus_tag="VW077_001135"
     CDS             complement(12654..14477)
                     /gene="dnaK"
                     /locus_tag="VW077_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9455159.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAESDKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14966..15490)
                     /gene="grpE"
                     /locus_tag="VW077_001136"
     CDS             complement(14966..15490)
                     /gene="grpE"
                     /locus_tag="VW077_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9455160.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15517..16551)
                     /gene="hrcA"
                     /locus_tag="VW077_001137"
     CDS             complement(15517..16551)
                     /gene="hrcA"
                     /locus_tag="VW077_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9455161.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16715..17224)
                     /locus_tag="VW077_001138"
     CDS             complement(16715..17224)
                     /locus_tag="VW077_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9455162.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCIATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
BASE COUNT         5418 a         3813 c         3140 g         5155 t
ORIGIN      
        1 ttacaaagag gacataattc ctgaacctac acaaataagt gttgctgctc ccccagttat
       61 cggaccagtc gcagcagcta atagtactgc tccaatacaa ccaccgattg cagatcctaa
      121 attgcctctt cctccactaa ctatttcgag ttcttcatta tccataacag aaaattgttc
      181 catcattttt gtattcatga caaatactcc ttttttcttt ttttattttt gtcttgttgt
      241 aactttgata agtttagtat atcatcgttt tttaaaattt ttcatccaga tcttgaattg
      301 tcatcgaaac gtcttgaatt agctttttta tttcaagcca cctctaaatg tttaaaaaaa
      361 ataatttcta atcacttttt taccattcag gaagttttaa tgactattca agatttcata
      421 aaatatgaac ttagttttat gacataatag acctatccac tatatgaaag gaattgccaa
      481 tgacttctta taaacgtaca tttgttcctc aaatagatgc gagagactgt ggtgtcgctg
      541 ccttagcctc gattgctaaa ttctatggtt cagatttttc tctagctcac ttgagagaac
      601 ttgcaaagac caataaagaa gggacgactg ctcttggcat tgtaaaagcc gctgatgaaa
      661 tgggctttga aacaagacct gttcaagcag ataaaacgct ctttgacatg agtgatgtcc
      721 cctatccatt tatcgttcac gttaacaaag aaggaaaact ccaacattac tatgttgtct
      781 atcaaacaaa gaaagactat ctgattattg gtgatcctga cccttctgta aaaatcacta
      841 aaatgtcaaa agaacgcttt ttctatgaat ggactggagt agctattttt ctagctacca
      901 aacccagcta tcaaccccat aaagataaaa agaatggtct actaagcaag cttccttcct
      961 ctgattttca aacaaaaatc tctcattgct tacattgttc tctcaagctt attggtcact
     1021 attatcaata taggtggttc ttactatctc caaggaatct tagatgaata catcccaaat
     1081 cagatgaaat caactttagg aatcatctca gttggtctgg ttatcaccta tatcctccaa
     1141 caagtcatga gcttctccag agattatctc ctaaccgttc tgagccagag attaagcatt
     1201 gatgtgattt tatcctatat tcgccatatt tttgaacttc ccatgtcttt ctttgcgaca
     1261 cgtcgtacag gagaaatcat ttcacggttc acagatgcta actctattat agatgccttg
     1321 gcttctacca ttctttctct ttttctggat gtttctattc tgattcttgt agggggagtc
     1381 ttgctggcac aaaaccctaa tctcttcctc ctttctcttc tttccattcc tatatacatg
     1441 ttcatcatct tttcttttat gaaacctttc gaaaaaatga accatgatgt catgcaaagt
     1501 aattctatgg ttagctctgc cattatcgaa gatatcaacg ggattgaaac tataaagtcg
     1561 ctcacgagtg aagaaaatcg ctatcaaaat atagacagcg aatttgtaga ttatttggaa
     1621 aaatccttta agctcagtaa atattctatt ttacaaacga gtttaaagca gggaacaaaa
     1681 ttagttctga atatccttat cctatggttt ggcgctcaat tagtcatgtc aagtaaaatt
     1741 tctatcggtc agctgattac ctttaacaca cttttttctt actttacaac tcctatggaa
     1801 aatattatca acctccaaac caaactccaa tctgcgaagg tcgctaataa ccgtttgaac
     1861 gaagtctatc tagtcgaatc tgaatttcaa gttcaagaaa accctgttca ttcacatttt
     1921 ttgatgggcg atattgaatt tgatgacctt tcttataagt atggttttgg acgagatacc
     1981 ttaacagata ttaatctcac gattaaacaa ggagataagg ttagcctagt tggagttagt
     2041 ggttctggta aaacaacttt agccaaaatg attgtcaatt tctttgaacc ctacaaaggg
     2101 catatttcca tcaatcatca ggatattaaa aacattgata aaaaagtctt gcgccgtcat
     2161 attaattacc taccccaaca agcctatatc tttaatggct ctattttgga aaacttaacc
     2221 ttgggcggta atcatatgat tagtcaagaa gatattctaa aagcttgtga agtagctgaa
     2281 atccgtcaag acattgaaag aatgcctatg ggctatcaaa ctcagctctc tgatggagct
     2341 ggtctatcag gaggacagaa gcaacgaatc gctctcgctc gtgctctttt aactaaatct
     2401 cctgttttaa tactagatga agctactagc ggtcttgatg tcttgactga gaaaaaagtt
     2461 atagataatc ttatgtccct aactgataaa accattctct ttgtagccca tcgtctcagt
     2521 atagccgaac gaaccaaccg tgtcattgtt cttgaccagg ggaaaatcat tgaagttggt
     2581 agccaccaag agttaatgca ggcgcaaggt ttctaccatc atctattcaa taaataagga
     2641 gaatgtcatg aatcctaatc tttttagaag cgtcgagttt tatcagagac gttaccataa
     2701 ctatgcgaca gtgttaatta tacctctttc attactattt actttcatct tgattttctc
     2761 ccttgttgcc acaaaagaaa ttactgtcac ttcccaagga gaaatcgccc ctacaagtgt
     2821 catttcatct attcagtcaa ccagtgataa tcctatcctt gctaatcatt tagtggcaaa
     2881 tcaagtagtt gaaaaagggg acttactcat caaatactct gaaacaatgg aagaaagtca
     2941 gaaaactgcc ttagcaactc aattacaaag acttgagaag caaaaagaag gacttggaat
     3001 tttgaaacaa agcttagaaa aagcgactga tcttttttct ggcgaggatg aatttggcta
     3061 ccataatacc tttatgaatt ttactaaaca atcccatgat attgaactgg gtatcacaaa
     3121 gactaacact gaggtttcaa atcaagctaa tcttgccaat agcagttcat cagctattga
     3181 acaagaaatt acaaaagttc aacaacaaat tggagaatat caagagctga gagatgctat
     3241 cataaataac agagcacgct taccaactag caatccacac cagtcaattt tgaatcgtta
     3301 tcttgtagcc tcacaaggac aaacacaagg aacggcagag gagccatttt tatctcaaat
     3361 taatcaaagt attgcaggtc ttgaatcatc tatcgcaagc ctcaaaattc agcaagctgg
     3421 tatcggaagt gtagcaactt atgataatag tttagcaacc aaaattgaag tactccgcac
     3481 tcagttttta caaacagcct cacagcaaca actaaccgtg gagaaccaat taacagaatt
     3541 aaaagtacaa ctagatcaag cgacacagcg cttagaaaac aataccttaa ccgctccaag
     3601 taaaggtatc gttcatctga acagcgaatt tgaaggtgaa aatagaattc caactggtac
     3661 agaaattgct caagtattcc ctatcatcac agatacaaga gaagtactaa tcacttacta
     3721 cgtagcttct gactatctac ctctactaga taaaggacaa actgtaagat taaaactgga
     3781 gaagattgga aatcacggca ttaccatcat cggccaactt cagacaattg atcaaactcc
     3841 taccagaaca gagcaaggca atctctttaa attaaccgct cttgcaaaac tatctaatga
     3901 ggatagtaaa ctcatccaat atggcttaca aggtcgcgtc actagtgtaa ctgcaaagaa
     3961 aacatatttt gattatttca aagataaaat tttaacccat tctgattaat tttcagataa
     4021 cactctataa ctatttatta tcttatcaaa aaggagaatc ataacatgga taagaaacaa
     4081 aacctaactt catttcaaga actaacaact accgaactca accaaattac aggtggagga
     4141 tggtgggaag aactcttaca tgaaacaatt ttaagtaaat ttaaaatcac aaaagcactt
     4201 gaactaccta ttcagctata aaaacaagac cgagaaacaa gtactctcgg tcttattttt
     4261 catcattctg tatgtatcac agtaagtacc tgacgaaaga cttgattttg acaggtggta
     4321 tttagactgg tattaggatg gctttccaca atcttcatga cggtatagag accaactcct
     4381 ctctcctccc ctttagaact ggctccaaag gagaagattt cagaaatatc gatgccctct
     4441 tctttgatgg agttttcgat gataaaggtc tcctgtgctc cattttttaa aaaggcgatt
     4501 gaaacatgag gttgactagc ttccacactg gcttcaatag cattgtcaca aaggatagac
     4561 acaatggtta gaaaatcaag tagactcatc ccctcgacct gaatctcctc aggaacttcg
     4621 acattaaaga caatgttctt atctctggct tttaaaaatt tccctgctag aagacttttg
     4681 aaggctttat cacgaatatt taccaatctg cccaggtcat atttattgtt ctgcaatttc
     4741 tgactggaat cctttaagac ggagccatag acctctttta tctgctccat atcctcctct
     4801 tcaatgccca gacgtaagct agtcaagagg ttggtataat catggcgaaa actccgtact
     4861 tccttgtaaa gctcctctat atgccgacta tagcgttcca tatctctata gcgcagggcc
     4921 tgctcttgtt ccaatctctc atagagtttt tccttcaaat aggtatccaa tttcttgata
     4981 acccccataa aaaagagtag gtaaaagact aggatgagat ggcgaacagt ctttgattga
     5041 atactttgtt catattcaaa aaaagacaga ctttccatga ctagatagta gccacccatt
     5101 atccagttaa tctgagccag ggacttttga aaggctttat cgagaatctc ctttctcaag
     5161 ctagtaaaat cgtagtccaa ccatttcaaa aaagctagag aaatgaagaa attgaaaatt
     5221 attatacata acccagtaaa tgagtagcca tcatatactt gcccttgtcc caaaaatgga
     5281 agcacaaaat aggagactcc tctataaaag agattcacca atatcattgg aaagagacca
     5341 taaaagaaaa ggagtttttt aggaagccct ctcaataata agaaagataa gcctatgccg
     5401 tacaagggtt ccataaaata agataggtaa acatttccta ctatatagct aatcatcaca
     5461 aaaacaaagg ccaacagtat cttaaaaaga aaggccttaa aaatcctctc gaaagtaaga
     5521 tcaattccat ccaccttaaa gaagatgaca atttctagtc cattagtaac aagtgtatac
     5581 aacaatatcc aagcaatgtt cataaattct cctagctcag tgtaatttat tgatggcctc
     5641 agacacttcc ctgaccttat aacgggcgat tagacaactt ccaccattgg gaaagaaaag
     5701 cagtttttct ttcttatcca aatgcaccac atttgcagga ttgatgagaa aagagcggtg
     5761 gcactgcaag agacggggct cctgcttgaa aacctcctct aaactcgctg taaattccag
     5821 cctgtctgtc ttggtataga gaataacacg atgggctctg ggcgacgttt cgagatagta
     5881 aacctcttta aaaggatact gaaattgggc aaattttgat ttaaagtaaa agcaatcttc
     5941 cgccagactt ttactatctt gactattggc atagaggagg gctgtctcga tccgagattc
     6001 aaactcctct gctgacaagg ccttatcaat gtagtccaaa gcagacactt ggtagcgaaa
     6061 agacaggggc ataaactccg agtgagtcgt cacaaagaca atcagggcat aaggatcccg
     6121 atcccgaatc tttctagcca cttccagtcc cttcatctct tcatttcgaa tctcaatatc
     6181 caaaaagaat agctgatggg cccccttctc atgcacttca gccagcagtt ggtccggctt
     6241 gccaaatacc tcaaaagagc taggaatgat atgatgtgct ttcaaaagtt tctcaatcgt
     6301 cgtttcaatt ctagtctgtt gggaaaaatc atcctctaaa acaaatattc tcatcttctt
     6361 actctccctc tttcaaccat ttttgacgaa tttctctata gcgacgtctg gcatacttga
     6421 cagcgtattg accttcttct ccgagaaaaa ggattctttc ttgaaaatca atcgatttta
     6481 atttatcaag attaaccaaa caatttcgat gacatctcat caaggtttcc ccacattggt
     6541 tctcaagatt acttaaattt tgaagcatat taaaactagc tttttctgta acaatctgta
     6601 cggtatgggc tttagttgga tgtgtttgga tatagtagat atcgtcgacg tttactttat
     6661 agactgtttt ctgcgtctga ataatcatat gtttcattgg attctcctcc taagaaaaga
     6721 ataacagaaa agttctagcc caagcccacc caatcctaaa cagtctttga aagctcctaa
     6781 aaggattatt tttctacaac atcatgatga gcttacaact attttatcat aaaattcact
     6841 cagcgcaatt caagacattt caatgacaat taaagatttg aataaaaaaa aagaattaaa
     6901 agtgataaac taacagtatc aaaacacagt tgccagaagt aagactggca cccagattaa
     6961 aggaggcatt cccatgacag atacagaccc catcaaaaga gctcatactt tgattactga
     7021 cttaaacaaa gcctaccaag catgcaaaca ggcaagtgct gacgacgtcc gctttcagga
     7081 gcaattaaac tctattcttg gttttctagc caaggctgaa acagtggata atcgattctt
     7141 gattgaacta gaaaaatttt accagacttc cagtcttctc atgggactca gcgctcttga
     7201 tccagatgct ccaactcgcg ccgcttggcg ggcctatgac cgcttccact ttgaccaagt
     7261 caagaccaag ttaatactca atgaaaatca aagagcaaac taggaagcta gccgcaagct
     7321 gtacttgagt acggtaaggt gacgctgacg tggtttgaag agattttcga agagtataag
     7381 tctctacgga ccaaccatta tcctgtagta acaaaaaaga agctgagaca gactgaactc
     7441 aacttctttt ttagtgtcgt atcagcaatg ttagtcctgc atctgacgtt ttacctgata
     7501 aggcaaatac aagactagta aaaccaaacc agctcctagt aaggctagaa gggccagttt
     7561 ttcttggaag gtaatcaacc caacaactaa ttccacaagt aaaacaaaac tggtcaatcc
     7621 tcgaactacc tcctgtaagc ctttttcctt ttgccccttg tccagtggaa agagttgggt
     7681 caaatactgg tagtcaaagg catgatagag ggccagcaac tgaaagagca agaggtagtt
     7741 aaagagaacc accactgctg tcgcaatcca agcttgctcg ataaaaacct gcgccagtac
     7801 ggaaagtaag agaagacgaa gactgagagc aaagaggtcg ccatttcgca gataagaacg
     7861 cagatagaga ttttgccaaa tcttcccagg caccttctga acagccttta agataaagtc
     7921 cagataggca cgacgcttga cactgtttga aattcccttg acctgcgtaa agagggcaaa
     7981 gaaacgaagc aagacttgct tacgcttgct ttcttgagaa ataagatagt cccagtccag
     8041 tccagtttca gtgaaaaatt tgctggcctt ttgacgaaag tggaaatatt ttcctacccc
     8101 caataaaagc acatagagca gaaaaactgg caagccataa cccattgcta aaaacaaggg
     8161 cgcaaataac agcaagaaaa gggtctgtac aaagagccaa aagactaggg aaatgccagt
     8221 ttgacgcttg agatggagct tgatttcctc ttctccaact aagagaaaga gcttgtctgg
     8281 agcctccata taggtggcaa ttcctcccca aagtaaaagt aaaacagacg taattcctac
     8341 aaacaaaaga ataggccaat gattttcagg aaaatgttgt aagagttgac tgtactggta
     8401 ggctagaaag cccaacagga caagcaggaa taagacaaag tggtcattga gcacatagcg
     8461 cagataacca agacactcct tacgaaaggc ctgctttctc tttaaaaaca agtctttcat
     8521 aggtcctcct cttttgtcag agccaagtaa atatcattca aactagcctc aggcatatca
     8581 aaggcttcgc gtagttgcag gagattgcct ttggcacgca cctctccctt gtgaagaatg
     8641 acaaaggcat cacacatctt ctccgccgaa tccagcacgt gggtactcat gagaatagac
     8701 ttgccctttt gcttctccac ttccaaaagc tgaatcaaat cagaaatagc cagcggatca
     8761 agaccaagga aaggctcatc cacgatgaaa agacttggat ccaccacaaa agcacagata
     8821 atcatgacct tctgcttcat cccttttgaa aaatgaacag ggaaccagtc taatttctgt
     8881 tccagacgga gcatctttaa caagggctct actcgttcga aagccacttt ttgctcaata
     8941 ccataagcca tagcaaccgt ttcgatatgc tctctgaggg tcaattcctc atacagacta
     9001 ggcgtctcag gaatgtagcc aatctgcttg cggtagctag tcgcatctcc ttgcagagtc
     9061 aggccattga tattgatgga gccactataa ggtgccaaca gaccgataat ctcattgatc
     9121 gtcgttgatt tcccagcacc attgagacca atcaaaccga ccaactgccc actttcaaca
     9181 gtaaaggaca catctttcaa aacaggaaca tgaacatagc cacctgtcag gtttttaatt
     9241 tctaacatat tttctccaaa tctggtataa tgtagctata ttatatcaaa attcaataca
     9301 gcagaggtag attttatgtc agattgcatt ttttgtaaaa tcatcgcagg ggaaattcct
     9361 gcttcaaaag tatatgaaga tgagcaggtc cttgcctttc ttgatatctc tcaagtaaca
     9421 ctaggacaca ccttggtcgt gccaaaagaa cactatcgca atcttttgga gatggatgct
     9481 acgagcgcca gccaactctt tgcccaagta ccaaaagtag ctcaaaaagt catgaaagtc
     9541 actaaggctg ctggtatgaa tatcatttcc aactgtgaag aagtcgctgg tcaaacagtt
     9601 tttcatactc acgttcacct tgtgcctcgc tacagtgctg acgatgacct caagattgat
     9661 tttatcgccc acgaaccaga ctttgacaaa cttgctcaaa tcgctgaaac catcaaaaac
     9721 gtttaaggag ttgctatgaa attatccaac ctactgctat ttgcaggagc tgcagccgga
     9781 agttatctgg ttacaaaaaa tcgccaaacc atcacagatg aagtcttgaa taccactgac
     9841 cgcgttcaag ctatcaagga cgatgtggat attatccaaa acagcctgca aatcattaac
     9901 cagcaaaaag aacttatcaa ggaataccaa gaagacttga cttacaagtt taaggtcttg
     9961 gaaaaggata tccagactag actagctgtg ataaaagaaa tgcagggaac tgaagataag
    10021 taaaaagagc cccccggctc ttttttaaaa cgtaaacaag ttatagtgaa ttgaatttag
    10081 aatagcacac catagtttct aaagcattgt tagagattac tttaaaatcc tcttatcaat
    10141 ttgttcatat tctatttcaa tctattatat atatcccatc aactatgcat cataatttag
    10201 gtaactcata ctcaataaaa atcaaaaagc aaactagaaa gctaggcaca gactgctcaa
    10261 aacaccgttt taaggttgtg gatagaactg acgaagtcag ctcaaaacac tgttttgagg
    10321 ttgtggatag aactgacgaa gtcagtaacc atacccatgg caagccgacg ctgacgtggt
    10381 ttgaagagat tttcgaagag tattattcac catttcacaa ctaaatcgaa gactttctgc
    10441 cgtaatgaaa acacctgaaa cagcttggtt tcagctgtcc ggaaacttta aaacttaggt
    10501 tcaaagttta ggtatggaac ttcgcaggag gtcactaccg tccgtcatta cttaggaaag
    10561 tcttaaaaaa tctataaagt aaaaagagcc cgacagctct ttttgtttat actcaatgaa
    10621 aatcaaaaag caaactagga aactagccac gggctgctca aaacactgtt ttgaggttgt
    10681 agatacgact gacgaagtca gtcacatata tacgacaagc cgacgctgac gtggtttgaa
    10741 gaaatttttg aagagtatta ttctccatca aaggcatctt taatatggtc aaagaagcct
    10801 tttttctttg gatttacttt caagtcacca gcagccgcga attctttcaa ggctactttt
    10861 tggcggtcgt tcaagcctgt cggtgttacg acattaacag taacgtattg gtcaccaact
    10921 gcaccgccac gaaggctcgg tgccccctta ccacgtaggc ggaatttctt accagtctga
    10981 gttccctctg gaataaccaa ttcaacatca ccgtgaacag ttggaatatc tactgtatca
    11041 ccaagagccg cttggacaaa gttgaggttg agattgtaga agatagtcgt tccttcacgt
    11101 tcaaacttgt cgctagcttc tacagaaact actacataca agtccccata aggtccaccg
    11161 ttaaagcctg cttcgccttg accagcgagg cgaatttgtt gacctgtttc cacaccagca
    11221 gggattttca catgtacgct atgagcttgt ttctcatggc ctgttccatg acaggttgta
    11281 catggatatt tgatttcttt tcctcgaccg tgacagacat cacaggttac ttggcgacgc
    11341 atcataccaa gaggagtctg cgtatcgacg ttaatgacac cagcgccatg acagcgtcca
    11401 caagtgactg gacttgtccc tggcttagca ccagagccat tacatgtacg acagccagct
    11461 tcacgatgat acttaacttc cttctcagtt ccgaagatag cttcttcaaa ggtcaaattg
    11521 acacgatact ggagatcatc tccttggcga ggagcgtttg gattgcgcga agaaccgcct
    11581 ccgccgaaga aacttgagaa aatatcctca aaaccaccga agccacctgc cccattgaaa
    11641 ccaccgaaac caccagctcc accaaaacca ccattggcgc ctgcagcacc atactggtca
    11701 taggcagcac gtttttggtc gtcactcaaa gtctcatagg cttcttgaac ttccttgtac
    11761 ttgtcctcag caccaggctc cttgttgata tctgggtgat attttttgga aagcttacga
    11821 taagcctttt tgatttcgtc tgccgaagcg tttttggata cccccagacg atcataaaat
    11881 tcagtattgt tcataattca agatacaaag ggcgtcaaac ggacacagcc atctaggagt
    11941 tttgacgacg gacgcttacg tcctagaaaa aactatcttt ttggcacagt ccgtagcccg
    12001 tgttcagtta cgaacaccct tattcctttc tattgatgtt ctgaatcgaa ccaccattta
    12061 ggtcgggttc aattcctttc ttttatattt agtaagaaat tctgaaaccc gtgttcagtt
    12121 acgaacactc tttgttcctt tctattgatg agacaaaacc caaacaaatg agattccgtc
    12181 cctttttatc gaaaaacaga ggctgggttg caacctctgc aaataagtaa attaacttta
    12241 ttttgatttt aaatatccta taagtacaga aatatttata tatttttgtc tcttcttagt
    12301 atttgcctat ataattccaa ttgatcataa gaaatacaac tactgtccaa atcataccaa
    12361 ttatgaacgg tattacaaat ttatatgttt gtatttctaa tgatatcata gctgatacca
    12421 tcgccgatac aaatgcagac aacaatactg aggtaacaaa attccttacc gcttcatcat
    12481 ttttgacaca actatcatgc tcagctaata taatttctct atatctagct aaatattcta
    12541 ttggaatatt atcaataaaa tcttttgatg ttctatagtt atcagcttga ttacaaaaat
    12601 cattataaac ttttcgtgta ttttttagat actcttcatc caatacactc atcttacttt
    12661 tccgtaaact ctccgtctac gacgtcatcg cctgcgtttc ctgttgcttg tgcgccttct
    12721 gctccttctt gagcttgttg cgctgctgcg gcttgttcgt agagtttaac agcaagtcct
    12781 tgagcttttt cgttcaatgc ttcaagtttt gctttcatgt cgtccaagtt gttgtcttct
    12841 tgagctttct taaggtcatc aagggcagct tgggcagcgt cacgttctgc gtcgaagcct
    12901 ttaccttcag tttccttgat tgtcttttca gtcgcaaaga ttgcttggtc cacttcatta
    12961 cgaaggtcta cttcttcttt acgtttctta tcggattcag cgtttgcttc tgcatctttc
    13021 atcatgcggt cgatttcttc gtcagtcaaa cctgagttcg attggatgac aatagtttgt
    13081 tctttttgag ttccaaggtc tttggcctta acagacacga taccgttctt gtcgatgtca
    13141 aatgttactt cgatttgagg aattccacga ggtgcagctg ggatatcagt caattggaag
    13201 cgtccaagag tcttgttatc tgctgccatt gggcgttcac cttgaagaac gtggatatca
    13261 acggctggtt ggttgtctgc tgctgttgag aagacttgtg atttagatgt tgggattgta
    13321 gtgttgcgat cgataagttt tgtaaatact ccacccattg tttcgatacc aagtgacaat
    13381 ggcgttacat caagaaggac aacatccttg acatcaccag taatcacacc accttggata
    13441 gccgcaccca tagcaactac ttcatcaggg tttactgatt tgtttggttc tttaccagtt
    13501 tcagctttaa cagcttcaac aacggcaggg atacgagttg aaccaccaac aaggataact
    13561 tcgtcgattt ctgacaagct caaacctgca tctgaaaggg cttgacgaac tggaactttt
    13621 gtacgttcaa caaggtcacg agtcaaatca tcaaatttcg cacgagttaa agtcatttcc
    13681 aagtgaagag gtccagcctc acctgcagtg ataaatggca agctgatttg tgttgaagtt
    13741 acaccagaaa ggtctttctt cgctttttca gccgcatctt tcaaacgttg cattgccatc
    13801 ttgtcagtag acaagtcgat accgttttct ttcttgaatt ctgctaccaa gtggtcaatg
    13861 attttttggt caaagtcgtc accaccaagt ttgttgtccc ctgcagttga caatacgtcg
    13921 aagacaccgt cacccaattc aaggatagag acgtcgaatg taccaccacc aaggtcaaat
    13981 accaagattt tttcttcttt gtcagtcttg tccaaaccat aagcaagagc tgctgcagtt
    14041 ggttcgttaa caatacgttc tacttcaaga ccagcaattt taccagcgtc ttttgttgct
    14101 tgacgttgag cgtcgttgaa gtaagccgga actgtgataa cagctttggt tactttctca
    14161 ccaaggtagt cttcagcgta gcctttcaag tattgaagga tcatagctga gatttcttgt
    14221 ggagtgtatt cttttccatt tgcagaaact ttttcagaag ttcccatctt agatttgata
    14281 gagataactg tatctgggtt tgtgactgct tgacgttttg cagcatcacc aacgatgatt
    14341 tctccgtttt tgaatgagac tacagatgga gttgtgcggt ttccttctgg gtttgcgatg
    14401 attttgcttt cagttccttc aagaactgca actgctgagt ttgttgtacc taagtcaata
    14461 ccgataattt tagacatgtg tttttctcct tgttagttaa ttttctattt ttctttttga
    14521 tactcttctt aagtggcgac agtggcgacg ccgtcagagc ttgacttcgt cagtctcttt
    14581 gactaaactt tgagcctaag gtctcaaaat ttgcgcaaat agcgccacgg cgaagagtat
    14641 cgtctttggt tcgcttcgct cactattaca aagcaaaaca tattttatct ttcttcatag
    14701 tttattgtcg tttcggacaa gttttctatt atgtaaattg cgacacgagg agtcgaaatc
    14761 gattttattt cgacgacgag ttagtaagga agctaggcaa acgccatagc aattgccgtt
    14821 ttctgacgag ttgctttagc taccgtcaga attgccgaat cgaacacgcc ctaagctctg
    14881 agtaaaaaag atagattttc ttgtgttcat cgaacacttc gttaatctcc tatttttact
    14941 gcgagctttt tacgggcttt gtatcttagt tatacaccac taccattgct gggcgtagga
    15001 tgcggtcatg gagtttgtag cctttttgaa agacttgggc gatggtatct actgggtgtt
    15061 catcgtctgc tgggagagtt tggatggcca tatggtagtt atggtcaaat tcgccatctg
    15121 ctgcgatttc ttcaattcct tcttctttca aagcgtgaat caagctttct tgcaccatcc
    15181 ccaagccctt cttcacatca tctgtcaaac cttcaactgc aagtgcacgc tcaaggttgt
    15241 caagagatgg taagattgct tttgccaagt cctggctacg ataacgttgc aagttttgac
    15301 gttcttcatt ggcacggcgt tggatatttt gcatttctgc atgagcgcga agatatttgt
    15361 tttcgaactc atctgcacgt tcatttgcca agtccaactc agacttttca ggagttgttt
    15421 cttcagctgt ttccacaact tcctcttctt gaacttcttc tacttcttca ttttttatat
    15481 cttgggccat gttcgcctcc tttaatgatt tcaatcttaa tgtacttcgt aatgattact
    15541 gctgaggtag cggtaaaaat ctgtcaactt catggtcaaa acacggttga ccacattgat
    15601 ttgattgatt agctgttggt aatccagatt aactggaccg ataatggcta gaattccaac
    15661 tccccgataa ggaatgagga acttactact aatcaccgct aggtcagcta aacaggactc
    15721 ttgaccgtct gcaacacgaa cattttgcat ctgatcctca cgcaacccct cacgaatctc
    15781 caaggccacc ttttgcggtt ggtcaaagaa ctgataggct gctagattgg caaaattcaa
    15841 gagattgacc ttgcccgcca tcacaatgtt ttcgttgaac atttccttaa agatgtgttc
    15901 aaagagatcg atgacattat ccgttgttgt aaagtaacgc tggataatct gcggaatctc
    15961 cgtccgaatc ttgtagtgaa tatctaaaac ggtgtgaccg aggaaacgtt cctgaatgat
    16021 gctcttcagt ttcagcaaat cctcctgcaa gaagttcctt ggaatcagaa actgactagt
    16081 aaccgttcgc gactcgtcta gggtaaatac cgccaaggct gtatgttgcc ccaaaacaac
    16141 gatatcaaag gctgtcaaac gttgcctgct cggctcaaca tccagtgcca ctaccgtaca
    16201 gccactcagg tctgttagca agttagcagc ctcttgcaga atatcctcca atttgaagaa
    16261 ttcctgatca aaggctttga caatctcata tacctcattt tcagccagtc ggtcaaaatc
    16321 cagtgagtgt ttcacatagt actgaaaacc agcaacactt ggcatccgac cgcttgaagt
    16381 atgagccttc tcaagcaacc cttgtttttc tagttccgcc atgtcattac gaatggttgc
    16441 actgctagag ttaatagact cttgcaaggc ttttgatccg acaggttcgt gcgttttggt
    16501 aaagatgtca ataatcagat ttaaaatatc ctgctgacgc tctgtaacca tctcatcact
    16561 cctctctgtc tgatcaatta gcactcaatg catccaagtg ctaacttatg attctattat
    16621 acacccttag aagagaatgt caagacaaaa actcaaaaaa ttagcactct tttataaaga
    16681 gtgctaaaaa tcaatctgaa gacctagaac cttgctattc atctactcgg tatttccttt
    16741 ttagtctgaa ataaccatga attagataag aaatcagaaa agcatataaa acaaaaataa
    16801 gaaagaaaga catagaaaga tcctctctaa acaaactcat acaaacaaca agaccaccta
    16861 agaaagtagc tatacaggaa cgaagtctag atcgcataac ttcccatttg agccccttac
    16921 tcatatagac ttgatcctct ggaagtaaat cagaagacga tttacgaaga atcgaacaag
    16981 aacctgctcc tatcaattcc caacctctat ctctaaaatc ttgcagttca cgcttatgct
    17041 ctttaagaaa tctagaatca tagatacgat agataacatc gtccggttga ccttgctcaa
    17101 aatagaacaa accaaaacga ctagttctat acctccaacc tttcaaatgc atctcatgta
    17161 aatattcttc tttcttgtcc aaatcaacaa tggtgaaaat ccgaaattct actctgctat
    17221 tcattgtctt accccaaaat tagaaaacat gcctagcgtt atttattaga taattctttc
    17281 cacttttgac tcaatctcca aaaaatataa gaaatctgaa tcgcaaaaac tatcaataaa
    17341 acccaatcta ttatgaaaat caaaaacact tcccaactga aagaactacc tccagtgaca
    17401 aactttgaga aaaacggtag tagagctaaa aagagaagca aaataggaag catccgcatt
    17461 gttaaaatcc gtttggcata aaaaatcttt atttaaacga aaatattatg gtaaaattta
    17521 cgccag
//
LOCUS       DAVKGU010000036        16377 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKGU010000036 DAVKGU010000000
VERSION     DAVKGU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.036"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            1..1046
                     /locus_tag="VW077_001139"
                     /pseudo
     CDS             1..1046
                     /locus_tag="VW077_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1119..1970)
                     /gene="srtC-3"
                     /locus_tag="VW077_001140"
     CDS             complement(1119..1970)
                     /gene="srtC-3"
                     /locus_tag="VW077_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HEU9455163.1"
                     /translation="MSRTKLRALLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
     gene            complement(1957..2850)
                     /gene="srtC-2"
                     /locus_tag="VW077_001141"
     CDS             complement(1957..2850)
                     /gene="srtC-2"
                     /locus_tag="VW077_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEU9455164.1"
                     /translation="MSKSRYSRKKSVKKKKNPFILLLIFLVGLAVAMYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTDQEKKQGVSEYAN
                     MLKVHERIGYVEIPAIEQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGQDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            complement(2843..3739)
                     /gene="srtC-1"
                     /locus_tag="VW077_001142"
     CDS             complement(2843..3739)
                     /gene="srtC-1"
                     /locus_tag="VW077_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEU9455165.1"
                     /translation="MAKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPAIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     RQSERALKALKEATKEVKVEDE"
     gene            complement(4078..5259)
                     /gene="rrgC"
                     /locus_tag="VW077_001143"
     CDS             complement(4078..5259)
                     /gene="rrgC"
                     /locus_tag="VW077_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEU9455166.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDNRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKADTVTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDGSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIVVTNLPLGTY
                     RFKEVEPLAGYTVTTMDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEENGHYTPVLQNGKEVVVASGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKTN
                     N"
     gene            complement(5306..7303)
                     /gene="rrgB"
                     /locus_tag="VW077_001144"
     CDS             complement(5306..7303)
                     /gene="rrgB"
                     /locus_tag="VW077_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus major pilin RrgB"
                     /protein_id="HEU9455167.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAGTTTTSVTVHKLLA
                     TDGDMDKIANELETGNYAGNKVGVLPANAKEIAGVMFVWTNTNNEIIDENGQTLGVNI
                     DPQTFKLSGAMPATAMKKLTEAEGAKFNTANLPAAKYKIYEIHSLSTYVGEDGATLTG
                     SKAVPIEIELPLNDVVDAHVYPKNTEAKPKIDKDFKGKANPDTPRVDKDTPVNHQVGD
                     VVEYEIVTKIPALANYATANWSDRMTEGLAFNKGTVKVTVDDVALEAGDYALTEVATG
                     FDLKLTDAGLAKVNDQNAEKTVKITYSATLNDKAIVEVPESNDVTFNYGNNPDHGNTP
                     KPNKPNENGDLTLTKTWVDATGAPIPAGAEATFDLVNAQTGKVVQTVTLTTDKNTVTV
                     NGLDKNTEYKFVERSIKGYSADYQEITTAGEIAVKNWKDENPKPLDPTEPKVVTYGKK
                     FVKVNDKDNRLAGAEFVIANADNAGQYLARKADKVSQEEKQLVVTTKDALDRAVAAYN
                     ALTAQQQTQQEKEKVDKAQAAYNAAVIAANNAFEWVADKDNENVVKLVSDAQGRFEIT
                     GLLAGTYYLEETKQPAGYALLTSRQKFEVTATSYSATGQGIEYTAGSGKDDATKVVNK
                     KITIPQTGGIGTIIFAVAGAVIMGIAVYAYVKNNKDEDQLA"
     gene            complement(7526..10186)
                     /gene="rrgA"
                     /locus_tag="VW077_001145"
     CDS             complement(7526..10186)
                     /gene="rrgA"
                     /locus_tag="VW077_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEU9455168.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYERKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDPENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAVGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GAPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVFYDTTEKRIRVTGLYLGTGEKVTLTYNVRLNDQFVSN
                     KFYDTNGRTTLHPKEVEKNTVRDFPIPKIRDVRKYPAITIAKEKKLGEIEFIKINKND
                     KKPLRDAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            10701..12230
                     /gene="rlrA"
                     /locus_tag="VW077_001146"
     CDS             10701..12230
                     /gene="rlrA"
                     /locus_tag="VW077_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEU9455169.1"
                     /translation="MLNKYIEKRITDKITILNILLDIRSIELDELSTLTSLQSKSLLS
                     ILQELQETFEEELTFNLDTQQVQLIEHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQ
                     SFNEFTQKEYISIATGYRVRQKCGLLLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFG
                     IEIYDLNDGSMDWVTHMIVQSNSQLSHELLEITPDEYVHFSILVALTWKRREFPLEFP
                     ESKEFEKLKNLFMYPILMEHCQTYLEPHANMTFTQEELDYIFLVYCSANSSFSKDKWN
                     QEKKTHTIQLILQHTRGKHLLSKFKNILGNDISNSLSFLTALTFLTRTFLFGLQNLVP
                     YYNYYEHYGIESDKPLYHISKAIVQEWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLP
                     AIPIFIILNNQADVNLIKSIILRNFTDKVASVTGYNILISPPPSEEHLTEPLIIITTK
                     EYLPYVKKQYPKGKHHFLTIALDLHVSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYL
                     L"
     gene            12495..13757
                     /locus_tag="VW077_001147"
     CDS             12495..13757
                     /locus_tag="VW077_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9455170.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGAFPSLIRLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQFW
                     GLIQDTLPHLNRTFKTTLSTFIRYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGY
                     RNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(13815..16139)
                     /gene="pflB"
                     /locus_tag="VW077_001148"
     CDS             complement(13815..16139)
                     /gene="pflB"
                     /locus_tag="VW077_001148"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9455171.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         4780 a         3605 c         2763 g         5229 t
ORIGIN      
        1 cgccgtttca agtgctatca ctgttcaaaa atgatggtcg ctgaaacttc tatcgtcaag
       61 aagaatcatc aaattcctcg tattatcaac caaaaaattg cgcaaaagtt gattgagaag
      121 atttctatga ccgatattgc tcatcagctg gccatttcaa cttcaactgt cattcgcaag
      181 ctcaatgatt ctcactttga gcatgatttt tcgcgtcttc ctgagattat gtcctgggac
      241 gttgaaacag tccggggagt gactgtttca atcgggagat ggagatgagc tttattgcgc
      301 aagattttaa caatctcaat atcatcactg ttcttgaagg tagaacacaa gctgtcatcc
      361 gagatcactt tcttaaatat gatagagccg tccgatgtcg cgtcaaaatc attactatgg
      421 atatgtttag tccttactat gacttagcta gacaactttt cccgtgtgct aaaatcgttc
      481 ttgatcgctt tcacattgta caacatctta gccgtgctat gagtcgtgtg cgtgttcaaa
      541 tcatgaatca gtttcatcga aaatcccatg aatacaaggc tatcaagcgc tactggaaac
      601 tcattcaaca ggatagccgt aaactgagtg ataagcgatt ttatcgtcct acttttcgta
      661 tgcacttaac caataaagag attttaaaca agcttttgag ctattcggaa gacttgaaac
      721 accactatca actctatcag ctcttgcttt ttcacttcca gaataaggaa ccagagaaat
      781 ttttcggact tattgaggac aatcttaagc aggttcatcc tatttttcag actgtcttta
      841 aaaccttcct caaagataaa gaaaagatta tcaacgccct tcaactacac tattctaatg
      901 ccaaactgga agcgaccaat aatctcatca aacttatcaa gcgcaatgcc tttggttttc
      961 gaaactttga aaacttcaaa aaacggattt ttatcgctct gaatatcaaa aaagaaagga
     1021 caaaatttgt cctttctcga gcttagcttt ttttcaaccc actacagttg acaaagagcc
     1081 ggaaaaagga acagccttag ctttcctttc atttcttttt atttccctcg tagtaaacgt
     1141 gctagcttcc acaaaacaaa caggattccc agaaatgcca gtaccactag cccacggtac
     1201 aaccattgag aggttgcaac acgcgataca gattgtcctt ctttcgtaaa agcaaccctc
     1261 gcaactgcag ctgtttgtgg atctgatttt tgataaacag cgactcgttc aaaattcact
     1321 aataagcgtt tattaaaggt aggaatcgga tcgcaggtta tcaaggtcat gatattttta
     1381 gagctaaccg attctaattt ttcccattcc gacggtaaaa taatctctgt gtccatcatc
     1441 tgatattcta caatttcctg gccattatca taataaagag catctccaac ttttagctga
     1501 tccaaatggc ggaaaaagac atggcttggc tctgcacggt gcccagcaat cactgagcga
     1561 atccctgtac catccagagg cagcggtgta ccatccacat gagccaagcc catccctaaa
     1621 tgatgataat ctgctcccaa ataaaccggc tccatgattt ccaaacttgg aatagacaag
     1681 taaccataga ctgcatcagg gtcgtcagac acttggtaat tgacctcata tccctccgcc
     1741 aaaaaaggat ctacaatgcg attttgcgaa gccaagcgtt gattgtaggc gagagaatgg
     1801 ttctgttgtt cttggtacat ttcagttgtc atggatttca caaatgtagc atgacctttc
     1861 acctgtccaa gagactgcaa caccatctgt ccaaaacaat aaataggaat caaacaggct
     1921 accaacatca acaagtatcc caataaggct cgtagtttag tccttgacat gacgcccctc
     1981 caattgcttt tctagtcctt tgacaatccg tcgattacga tacacgcgat acagcaagag
     2041 aaggatgacc gccatcgctc ctagtaataa ccacaaccag aattgcccac gctctctcac
     2101 cgctcgattc cgctcagcaa tcggcgctgt atatggaatc cgtttcccac gtaccaacaa
     2161 acggtggctg ttaatcatgt atggcgtaca agtcagtaaa gtcgcataat cttgtccatg
     2221 ttgaatcaga acaggctcaa aatcattcgg ctccaccgtc actatctgat ccacttgata
     2281 ggccaaaacc tggtctaaaa cgtgaagata aaagatatcc cctttcttca tcttatccaa
     2341 ttgactgaac aattctgccg ttggcaatcc tctgtgagca gtgatcactg tatgggtatt
     2401 ttcacctcca acaggcagcg aagccccttc taacagccct gcccctttct gaagaatgtc
     2461 ttcacttgtg ccaacataca tggggatttc ctgttcaatc gcaggaattt ctacatatcc
     2521 gatacgctca tgaactttta gcatgttagc atattctgaa actccctgtt tcttttcctg
     2581 atctgtaaaa ggatcgagga tttcagatgg tttcaaggtc gcattgaagg cttgagccaa
     2641 gcgccaacgc tcctcaagtt ccgccttatc catctgggaa accgtctcat caaactcttt
     2701 aataacctcg tttgactcaa tacgataata ataacgagac accaagggat acatcgcaac
     2761 ggcaagcccc accaaaaaaa tcagaagaag aatgaacgga tttttcttct ttttcacgct
     2821 ctttttccgt gaatatctac tcttactcat cctctacctt cacttcctta gtagcttcct
     2881 tcaatgcttt caaagctctt tctgattgcc gtttcttctt gcgcaagcgt cgaataatcc
     2941 ataaaagaat cacaatcaaa ccaactgcca cataaaacag gtagcgatag agatgactga
     3001 gtttgtttgc cgcaataaat tcttcctcaa cctctgctac gtacggtatc cgatgccccc
     3061 gaaccaatag acgatgggta ttgatcatgt atggcgtaca ggtcagcaag gtcacataat
     3121 catgacctgg tacaatcaat aaatcatcaa agttcgtcgg ctcaatcacc tttacttgat
     3181 ccacttgata ggccatcact tccttgatat tgtgcacata aaacttatcc ccaactttaa
     3241 gtttggtcaa atccgtaaac atcttagccg ttggcaaacc tgtatgtgcc gtaatcaccg
     3301 catgggtcga attgcctccg atcggtagag aagttccctc tagatgtcca gcgccttgct
     3361 gcaatacctc ttcagcagta ccagcataaa ccggcaaatc tacatcaata gcagggattt
     3421 ccacatgccc catccgctca tggatttcta acatgcgtgc atactctgct cgcccttttt
     3481 tcttcatttc ttccgaccaa ggatcgccac tcactacatt attcaaagag tcattgaagg
     3541 cttgtgccaa tttcattcgt tcatcaatgt cagcctcatc caacgttgct ttttccttat
     3601 caaagtcagc aatttgttga tttgattcca ctcgataata caagcgagac accagcggat
     3661 acgccattac cgccattcca atgaaaaata ccactcctaa taggagattg tttcgttttt
     3721 gcttttttgt ttttgccatt ttttatcagc attcctttaa cttcaaactc atcattttat
     3781 cgcttttcct aacagacaat cctatctgct aagagaaacg agaaaatctc atttctttct
     3841 gattcaccat ccattttaaa tcagaatccc agtcctgtcc attcctaaaa tgattaccag
     3901 accagtccta tcgttttaca aacaataacc cacaatgagg ctggcacaat ctgcccagcc
     3961 tcatatccgt tattatgatt catgccaaat caccatgatt cctgattaac atcacctcag
     4021 agtactctgg tcttcccttc agcctgctat ctttttcata atgatgtaca ttgaatatca
     4081 gttatttgtt tttttcgtaa gataatagcc actaccaaac aacaaaatgg caacaagcat
     4141 caagatatac aaggtttctt cccctgtatc tggcacatca atccgtggtc gcttgttatt
     4201 tttaaccact gttaccagtt ccttacgagt atctttcccg attgtaaagg aaacaggcga
     4261 tgttaattga acataaccag ttggagcttg gagctcccat aaatagtatg tcccatactc
     4321 tagaccttcc actcggaaac gaccatcttt ccctgatgcc acaactactt ccttaccatt
     4381 ttgaagaaca ggagtatagt gtccgttttc ttctttcatg actttgaaca ttgccccttg
     4441 aagagaggta ttggtcctac catccacctt cataaagtca acattgccac gtggtaattt
     4501 ctgattgaca accgtaatcg tcaccagctg atgatctacc aactggacat ccgtatccat
     4561 cgtcgtaaca gtatagcctg ccagtggctc cacctccttg aaacgatagg tcccaagagg
     4621 aagatttgtc acaacaatct ctccattttt atcagtatag agagttctcc ctacttgacc
     4681 agaagaactg taacggtatt ctccaatcaa gggaacctct ttttcagaac catctcttgc
     4741 tactgatacc aatttaaagc cgacaccctc caagcgattg tggtcttgat ccacctttat
     4801 cagcttcacc tttgttgtca ccgtatctgc ttttttcgct acaatgacca aaggctctac
     4861 cgtctgatct gtcatttcaa aaagaaattc agctggataa gaaaccgcat ccgtctggat
     4921 aatagagcga acatagtaaa gaccatttgg aatatgagat acttcaatct gattctcaag
     4981 gaaggtcatc tcaaacgaag tctttttgaa agaagaaagt ttattctcat cccacgaatg
     5041 caagtctctc acaatttgca cccgattatc ataggaatac gaatcatcca acttccatac
     5101 ctgcaaccga tgaccatcac gagatggcaa ttgactaacc acctcctgat agttctccaa
     5161 ttgcaagacc aacgtgtgat cttcttgcgc ttggactgca tgtgcacccc atacaagaga
     5221 aaaacacaga gccataacaa agaagatacg actaatcatt ttctgcattt tctgcattgt
     5281 catcaatggc tcctttctct cttacttaag caagttgatc ctcatctttg ttgtttttaa
     5341 catatgcgta cactgcaata cccataatca cagcccctgc tacagcaaag ataattgtac
     5401 caataccacc cgtttgtggg atcgtgattt ttttgttgac tacttttgta gcgtcatctt
     5461 tacctgaacc agcagtatac tcaatgcctt gtccagtcgc tgaataagaa gttgcagtga
     5521 cttcaaattt ctgacggcta gttagtaatg cataaccagc aggctgtttt gtttcttcta
     5581 agtaatatgt acctgcaaga aggcctgtaa tttcaaagcg accttgtgca tcagaaacta
     5641 atttcacaac attttcattg tccttatctg ccacccattc aaatgcattg ttggcagcaa
     5701 tcacagcagc attataagca gcttgagctt tgtcaacttt ctctttttct tgctgagttt
     5761 gttgttgtgc agtaagagcg ttataagcag caactgctct atctaaagca tcctttgttg
     5821 taacaaccaa ctgcttctct tcttgactca ctttatctgc tttacgtgct aaatattgac
     5881 cagcattatc agcatttgca attacaaatt ctgccccagc taaacgatta tctttatcat
     5941 taactttgac aaacttttta ccatatgtaa caacttttgg ctctgttgga tcaagtggtt
     6001 ttggattttc gtctttccag ttcttgacag caatttctcc agctgtagtg atttcttgat
     6061 aatctgctga ataccctttt atactacgtt caacgaattt atattctgta tttttatcca
     6121 atccgttaac agtaactgta tttttgtctg ttgtcaaagt tacagtttgt acaactttac
     6181 cagtctgagc attaaccaaa tcgaacgttg cttcagctcc agccggaatt ggtgcacctg
     6241 tagcatcaac ccatgtcttg gtcaatgtca aatcgccgtt ttcatttggc ttattcggct
     6301 ttggagtatt cccgtgatct ggattattac catagttaaa tgttacatca ttagattctg
     6361 gtacttctac aattgctttg tcattcaatg ttgccgaata agtgattttc acagtttttt
     6421 cagcgttttg gtcattcact ttagctaaac cagcatctgt taatttcaaa tcaaaaccag
     6481 ttgctacttc tgttagagca taatcacctg cttcaagtgc aacatcatca acagttactt
     6541 tcactgtacc tttgttgaat gccaaacctt cagtcattct atcgctccag tttgctgttg
     6601 cataattagc aagtgctgga atttttgtaa caatttcgta ctctacaaca tctccaactt
     6661 ggtggttcac aggtgtatct ttatctacac gtggtgtatc tggatttgct ttacctttga
     6721 aatctttatc aatttttggc tttgcttctg tattttttgg atacacatgc gcatccacaa
     6781 catcgttcaa tggtaattca atttcaattg gaactgcttt agaacctgtt aaggttgctc
     6841 catcttcacc gacataagtt gataaactgt gaatttcata aattttatac ttagcagctg
     6901 gtaaatttgc cgtgttaaat ttagctcctt cagcttctgt taattttttc attgcagttg
     6961 ccggcattgc ccctgagagt ttaaatgttt gtggatcaat attcactcct agagtttggc
     7021 cattttcatc aataatttca ttattagtat ttgtccaaac gaacataaca ccggcaattt
     7081 cttttgcatt tgcaggtaga acacccactt tattaccagc atagttacct gtttctaact
     7141 catttgcaat tttatccata tccccatctg ttgccaatag tttatgaacg gtaacagatg
     7201 ttgttgtcgt cccagccgca aaaactgttg cagctgaaaa caggctactc gctgtcaata
     7261 ataaggcagc aagcattgtt aaaaatttgt tgattgattt catgatttct ccttatttat
     7321 ctcttaaaat caactgtgag caatgtaatg aattacacat gacaataata atcctttttc
     7381 tgtgcaagtt ttcgaaaaca tctccccttt caatggtggt ttacttggcg aagaaaactg
     7441 gtttcaccaa gtaaacttaa atctcttctt gagtgctact tcttaaaagt attgttcaga
     7501 acatagatat tatcatttct acactttacg gatgtttccg tgtgtataat agaactcctc
     7561 ccatcatcat gcaacctatc agatagaatg gcaacattcc gataccacca gttcgaggat
     7621 attctctctt tggaggaata ggttcattgg taatatagtg cttatcattc gtaaactcgt
     7681 aacccgctgg tatatcttgt ggaacgattg aagtcacatc tctgacttct ccatttacta
     7741 tttggaaggc aacgataggc ttattttgaa cgggtttata accagctggt tcagaatttt
     7801 caaataatcg atatttccca tctgacagat ttttaaaggt caacttacca tcttcacctg
     7861 ttctcacatt ttgataagtg ccattttgat caatagctcc ataaatatct ggataatccg
     7921 gatgttgttt ttgaagacta aagaccgcat ctctcagtgg ttttttatca ttcttattga
     7981 tcttaataaa ctcaatttca ccaagttttt tctcttttgc aatcgtaatt gctggatatt
     8041 ttcgcacatc acgaatctta ggaatcggga agtcgcgcac tgtgttcttt tctacttcct
     8101 tagggtgtag ggttgttcga ccattcgtgt catagaattt attgcttaca aattggtcat
     8161 tcaagcgaac attataagtc aatgtaactt tttcacccgt tccaaggtac aaacctgtta
     8221 cacgaatcct tttctcagtc gtatcataga acacttttgc attttttagc aagccaccat
     8281 cattttgtgg accacctaca gcttgtccat tctccaagcg actaccatcg tttgcagtta
     8341 aagtgtaatc tgctggatca aatcttccat ctgtgcccaa ttgcaaatca attaactcac
     8401 ccatcggatc tgtaatcgta ccattctcaa ttgatttctt ttcagttacg atggtgtgga
     8461 aataacgatt caactgttcc aatatttttg tcgtgtcagt aacattggta tagttttcag
     8521 gtttactaga aatactttgc ataaaactag tagccgttgc ttcatccgta ccaggatctc
     8581 cgttaatacc aatacctacc gtaaagacat catacccatc aggagcaata ttcccgttat
     8641 agtaccatct tgtaggggca ccatgattgg taattttagc agtattagaa ttaaacggat
     8701 aagccagaat actctctctc caattaagcc aaatatatcc accattaatt ggatcgccta
     8761 taactgcata accaaccgcc ttcatttcag aatatttttc aggtttaact gggaaagctg
     8821 caggagcacc tttttcataa actgtcttat ctgtaaacat ctggtaactt tgcccatctc
     8881 cgcgtacaat tgtatgttct ccactagttg cttgcgtaat aaaatcactt aatagtattc
     8941 catctttatt aggagattta ctaaaaaatg catttagttg attttgatat gatggagcaa
     9001 acgtagcatg attaaaatta atcggatacg acatagttgg gacaccatcc gtaatatgga
     9061 aaatgacttt ttgactattt tgtctcgctt gttgtgtcaa aatctcatct gccttcatca
     9121 aagctttctg agtaaaagtg gcaccgaatt ggtacatcaa tctatttcca tcatgatctt
     9181 ctgcctcggt aggtacctta ttttttaatt ctacaatgtc attcttatca ttagtcagct
     9241 ttaaataact ataatctttg gtattggttg taaaactcgt ctgatcataa ttccaaaaaa
     9301 gagaatcatt caatcgtttt ccgtttttat ctgctacccc tttttctact gtaaactcgg
     9361 tcccatcaaa gatagtggaa gcataagtca caagcgctac cctattttct ggatcagatg
     9421 taattttatc aataagagaa cgtgtcgcct caccagctct ttccgcacgt cgagcattct
     9481 tgtttcgaat gttactcata ctatttgagt tatcgagcaa gataacgaca tccagcggca
     9541 cagacttatc ttttcgttca tacactgttt tcccactaac cgtcaattcg attccatatt
     9601 ggttatcatc caaattattc acttgataaa ttctctttga aagtgtacct tctggaatca
     9661 cacgttcata tggattcgga ttcaacgcct tgtgctgtcc gtttttttcc gaaccatcta
     9721 ccttaataat ctgataaggt gtttgaacat ctggataagt ccctgtttgt ggatactggt
     9781 cagatagagc ctcttctcga ttttctacct gttcaccttg gacagtcgtc cgaccattct
     9841 tctcaacttc aacagtccat tgtttagtag agggtttata accaactgga ggttgggctt
     9901 ctgtcaaggt gtatgtccca ggttttatgt ttgaaaatat cgcttctcct gtttgcgcct
     9961 ctgtcctttg cgaaacagtt gtgccatccg tattgttttt caactcaaag acggcatctc
    10021 ctagaagcgc tcctccttcg cctgtctcct taatcactac ttttcctatc gctggactgg
    10081 tttcaggcgt ttctgctaaa gcaactatcg aagaaaaagc tgtcaactga gatatacagc
    10141 acagtcctgc aactgccttc tgaaatatct ttcttacttt tttcatgtgt gtctccctgt
    10201 taagcattat tcatagaaca agttaatcct tctatgattc ataggtactt tcaatctgta
    10261 tacacgaaga aacggattac ttatgttctg ataaaattta tctcactgag ttaatccgtt
    10321 cactaacaaa cgtagcaaaa gcgtataaaa gtgcgttata gctatcttct caacctggca
    10381 atgacacact tgtatacaat atagtatagt atagtattac tattaactat cctagtataa
    10441 attaaaacaa cttccatcac aatagatatt tttatcacat atttttttat agaacgacaa
    10501 tatttgataa aaaatatttt ctaataaaaa aaataagcca taaaactcca ccaatactca
    10561 ttactatctt taaaatgcct tttattacat gctgttttat caataaagtc ggaaattcca
    10621 caaccttttt gacaagacaa ttagtttctt ttgtattata ataattaaaa ttatctatat
    10681 gcaactaaga ttcggtaact atgctaaaca aatacattga aaaaagaatt acagataaaa
    10741 ttaccatttt aaatatatta ttggatattc ggtctattga actagatgaa ctgtctactt
    10801 taacttcatt acaatctaaa agtctcctaa gcattttgca ggaattacaa gaaacattcg
    10861 aggaagagtt gacatttaat ttagatacgc aacaagttca attaatcgaa caccattctc
    10921 atcaaacgaa ttattacttc catcagcttt ataaccaatc aactatttta aaaatactcc
    10981 gcttctttct actacaaggt aatcaaagct ttaacgaatt tacacaaaaa gagtatattt
    11041 ccatcgcaac aggctaccgc gtacgacaaa agtgtggctt gttactacgt tcagtaggtt
    11101 tggaccttgt gaaaaatcaa gtagttggtc ctgaatatcg cattcgtttt ttaattgcac
    11161 tcctccagtt tcattttggt attgagattt atgatttgaa tgatggaagt atggattggg
    11221 tcacacatat gattgtgcag tctaattctc aactcagtca tgaactgtta gaaatcacac
    11281 ctgacgagta tgttcatttt agcatactag tggcactcac ttggaaacgt cgggaatttc
    11341 cacttgagtt ccctgagtcc aaagagtttg aaaaattaaa aaacctcttt atgtatccga
    11401 ttttgatgga acattgccaa acttacttag agccacatgc caacatgact tttactcagg
    11461 aagaactgga ttatatcttt ttggtttatt gttctgctaa ttcttcgttt tccaaggaca
    11521 aatggaatca agaaaaaaag actcatacta ttcaactgat tcttcagcat acaagaggaa
    11581 agcatctact atccaagttc aagaatatct taggaaatga catttcgaat tctctttctt
    11641 ttttaacagc actgactttc ttaacaagaa cgtttttgtt tggtttacaa aatctagttc
    11701 cctattataa ttattacgaa cattatggga ttgaaagcga caagccactg tatcatattt
    11761 cgaaagcaat tgttcaagag tggatgactg agcagaagat tgaaggagtt atcgaccagc
    11821 atcggctgta ccttttctca ctctacttga cagaaaccat cttttcctca ctacctgcta
    11881 tcccgatttt tatcatttta aataatcaag cagatgtaaa cttaataaag tccattattt
    11941 tacggaattt tactgacaaa gttgcctctg ttacaggata caatattcta attagtcctc
    12001 ctccttcaga ggagcatttg acagagccat tgattattat tacaacgaaa gaatacctgc
    12061 cttacgttaa aaagcagtat cccaaaggga aacatcactt tttgacgatt gccttagatt
    12121 tgcatgttag ccaacagcgt ctcatctatc agacaattgt agatatccga aaagaggctt
    12181 ttgacaaacg ggtcgcaatg attgctaaaa aggctcacta tttgttataa gttcatcgta
    12241 ctgtctacac acaaaaaggg gcttcctaag ccatctctat tcttatcccc atatttccca
    12301 gttttgggta aatctgaaca gaaaaacact tctgctctct agtttctgct agtgaacaaa
    12361 agtgtttttt ctggctctat aatttctgta gtgggtaaat cccactgtgg agattatagg
    12421 gctttttgag tatcaaaaaa gtcccataag acttataatg aaaagcgcct aaacaactca
    12481 ttagaaagta tcttatggaa cacatcaatc ataccacatt actcatcgga ataaaagaca
    12541 aaaacatcac cttaaataaa gccattcagc acgataccca tatcgaagtc ttcgctacac
    12601 ttgattatca cccacctaaa tgtaaacact gtaaaggaaa acaaatcaaa tacgacttcc
    12661 aaaagccttc taaaatccct tttatcgaga ttggtgcttt ccctagcctc attcgtttga
    12721 aaaagagacg atttcaatgc aagtcctgtc ggaaagtcac tgtagctgaa acaactctcg
    12781 ttcagaaaaa ttgccaaatc tcggaaatgg tgagacagaa aattgcccaa ctcctactca
    12841 acagagaggc tcttacacat atcgcttcta aattagccat ctctacctct acctctaccg
    12901 tctatcgtaa gctcaagcaa tttcatttcc aagaggatta caccactttg cctgaaatcc
    12961 tctcctggga tgaattctcc taccagaagg ggaaattggc tttcattgct caggatttca
    13021 acactaagaa aatcatgacc attcttgata acagacgtca aacaaccatc cgaaatcatt
    13081 tcttcaagta ctcgaaagaa gctagaaaaa aagttaaagt cgtcactgtt gatatgtctg
    13141 gaagttatat ccctctcatt aagaaattat ttcccaatgc taaaattgtt ctcgatcgtt
    13201 tccacattgt ccaacacatg agcagggctc ttaatcagac tagaatcaac atcatgaagc
    13261 aatttgatga taaatctctg gaatacagag ctcttaaata ttactggaaa tttatcctaa
    13321 aagatagtcg gaaactctct cttaagcctt tctatgctag aactttcaga gagaccttaa
    13381 ctcctaggga gtgtctgaag aaaatcttta ctctagtacc tgaacttaaa gattactatg
    13441 acctgtatca actactccta tttcatctac aagagaagaa tactgaccag ttttggggct
    13501 taattcaaga cacgttacct catctcaacc gcacctttaa aaccactttg agcacattta
    13561 ttcgctataa aaactacatc actaacgcca ttgaattgcc ttattctaac gctaaacttg
    13621 aggccactaa caaactcatc aaagacatca agcgcaatgc ctttggttat cggaactttg
    13681 aaaacttcaa aaaacggatt ttcatcgctc tgaacatcaa aaaagaaagg acaaaatttg
    13741 tcctttctcg agcttagctt ttcttcaacc cactacagtt gacaaagagc cctttttatt
    13801 attcaagaac ttgattagct caatgcatcc aaggcgtcat ccattgaaag aacttcgtgg
    13861 aagacacgtt gtgtcaattc agttttttgt tctggagtga ggtatttagt atttacacag
    13921 tatccagaga tacgtacgat aacgtcttca cctgacatga ttttttcgta aacatcgttc
    13981 aagtccataa cgttcaagtt aacgtgttgt ccaccgtttt cgaagtaacc atcaaggatt
    14041 gttaccaagt tatcaacttg ttcatcacga gtcttaccaa gagcgcgagg tgatacttgt
    14101 gtagtcaatg agataccgtc agctgcataa ctaaagtcaa ggctagaaag tgagttcaag
    14161 ttttgcaacc aaccaccttt agctttgtta gatgggttag cacctggtga gaagaattca
    14221 agtttagaca agttcacaga accatcttcg ttgaggtata cacctttgtg aactggtgag
    14281 ttaccagttt gtttagagta agcaacgtta gatgtgattg tcaaaagtga tactgtagct
    14341 tctgcgtctt tgtatagttt gtggctacgt agacgagttg tgtaagcttc gatcaaccat
    14401 tctgccaatt cgtttgaacg tgggtcatct tcaccccagc gtgggtagtc accgattgtt
    14461 tcgtaatcgt agatgtagcc atcttcgtca cggattggtt taactgtagc gtatttgata
    14521 gctgacaatg tatcaacagt gttagcaaat ccacagatac cgaatcccat gttggcacgt
    14581 tgtttagttg gcaagaaggc catttgaaca gcttcgtagt tgtacctatc agtcatgtag
    14641 tggatgatgt tcaaggcatc tacgtaagtg tcagtcaacc agtcaagaga tttttcaaag
    14701 ttcgctttaa ctgattcaaa ttcaagaact tcgtcacgga ttggttcgat atcaaatact
    14761 ttgtagtctt tgtgaacatc gtcgtaacca ccattcaaac cagtaagaag ggctttaaga
    14821 acgtttacac gagcaccgaa gtactggatg ttgtggcgtt gttcttcatt ttctggatca
    14881 agtggagaca cacagcatga gatacagctc atttcaccat atccgtcttt agccattgtt
    14941 gttacacctt cgtattggat agaagagtgt ttgtggctca tgtgcataca gtagcgacgg
    15001 aagttgtatg gcaatttgtc agtccaaaga actgtcaagt ttggttctgg tgagttaccg
    15061 atgttgtcaa gagtgttcaa gaaacggtag tccatcttag taacacggtg acgaccgtcg
    15121 ttacccatac cagccataga agttgtgata aaggttgggt cacctgagta caattggtca
    15181 taagcttttg tacgagcaaa tttaactgta cgaagtttca taacgaaatc atcaacgaat
    15241 tcttggattt ctgattcagt aaatgtacca cgagcaaggt cacgttctgc aaagatgtcc
    15301 aatacgattg gtacacgacc tagagatgta gcagcaccgt taatcacacg gcagacagcc
    15361 atgaaagcaa tgttaaccca ttggattgct tctttcacgt tcatcgctgg tttgcgaaca
    15421 tcaaccccgt aaaggtcacc caggcgaaca acttgttgca atgcttggta ttgaaggttt
    15481 acttcttcac gaagacggat tgtttcttca tcgatttctt tgattgcatt ccagtcattt
    15541 actttttctt gcatcaagta gtctgcaccg taaagagcaa gacgtgcgta aacaccgatg
    15601 atacgtccgc gtgagtatgc atctggaaga ccagttacag tgtgtgcgtg acgagcgcga
    15661 cgaatatttg aagtgtaggc acggaaaata ccgtcgttaa ctgttgttac atatttagtg
    15721 aagatttcgt gaacagctgg gtctggttcg tatccatttt ctttcaaagt agtttcagcc
    15781 atacggatac caccttttgg catgaagttc aatttgaaga gttcatcgtt ttggataccg
    15841 aagataactt cattttcttt gtcgataaat ccagcaggga tatcagcgat agatgttgga
    15901 cgagtgtcca ttgggaaacg agtttcttcg tagtgtgctt tagtttcttc tacaattttc
    15961 ttgatgtgaa gtgaacgctc tgttggtcct gcaaggaagc tttcgtctcc atcataaggt
    16021 gtgtagttag cttgtacaaa tcgtgataca cttgcttttt ctttccaatc tacgcctttg
    16081 aagccttccc aagctttgtc aaaaatatct tgtgcttcaa caactgtctt aacaaccata
    16141 ttaatgtcct cttttttcta gtaacagtca tctgttacat tcataagaca agtataccat
    16201 atagtaaccg attttaacaa gtgaaaattc cctattttta cactttcttt tctaaaacag
    16261 tctatatttt atttcaaact gtattatatt tttgaaaaaa taaagtcctt ttttcttttt
    16321 ttcagaaaaa agggtataat aaaagaaaat aagcagtaac actcaatgga aatcgaa
//
LOCUS       DAVKGU010000037        16187 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKGU010000037 DAVKGU010000000
VERSION     DAVKGU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.037"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            167..430
                     /locus_tag="VW077_001149"
     CDS             167..430
                     /locus_tag="VW077_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9455172.1"
                     /translation="MSKMSMSIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            423..701
                     /locus_tag="VW077_001150"
     CDS             423..701
                     /locus_tag="VW077_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9455173.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            722..874
                     /locus_tag="VW077_001151"
     CDS             722..874
                     /locus_tag="VW077_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455174.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            896..2137
                     /locus_tag="VW077_001152"
     CDS             896..2137
                     /locus_tag="VW077_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9455175.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGAWNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            2147..2377
                     /locus_tag="VW077_001153"
     CDS             2147..2377
                     /locus_tag="VW077_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9455176.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            2600..3322
                     /locus_tag="VW077_001154"
     CDS             2600..3322
                     /locus_tag="VW077_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9455177.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEIFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVEIFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILLASIA"
     gene            3434..4768
                     /gene="pepC"
                     /locus_tag="VW077_001155"
     CDS             3434..4768
                     /gene="pepC"
                     /locus_tag="VW077_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9455178.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(4824..5735)
                     /locus_tag="VW077_001156"
     CDS             complement(4824..5735)
                     /locus_tag="VW077_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9455179.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(5759..6562)
                     /locus_tag="VW077_001157"
     CDS             complement(5759..6562)
                     /locus_tag="VW077_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9455180.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(6590..7579)
                     /gene="manX"
                     /locus_tag="VW077_001158"
     CDS             complement(6590..7579)
                     /gene="manX"
                     /locus_tag="VW077_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9455181.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(7813..8832)
                     /gene="adhP"
                     /locus_tag="VW077_001159"
     CDS             complement(7813..8832)
                     /gene="adhP"
                     /locus_tag="VW077_001159"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9455182.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVAPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(9171..9983)
                     /locus_tag="VW077_001160"
     CDS             complement(9171..9983)
                     /locus_tag="VW077_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9455183.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            10173..11591
                     /locus_tag="VW077_001161"
     CDS             10173..11591
                     /locus_tag="VW077_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9455184.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLASLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQAK
                     DVHVMIWILDALFILNYISMAL"
     gene            11696..12403
                     /locus_tag="VW077_001162"
     CDS             11696..12403
                     /locus_tag="VW077_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9455185.1"
                     /translation="MIKKNIWDEVLNRGKWLIIFLVGLLFSQFPLALATFLTSRKFPL
                     LQTGLLVGTLSLVVLTVFIIGAKKTQLASFGLSFFKAKDLARLALSYLVILAFNILGV
                     VLLHLMNETTTSNQSNINDLVQNSSLISSFFLLVLIAPICEEILCRGVIPKKLFRGKE
                     NVGYIVGAIIFALLHLPTNLPSLLIYGGMSTVLTWTVYKTQRLEMSILLHMIVNGIFF
                     CLLALVVILSRTLGISV"
     gene            12498..13478
                     /gene="folP"
                     /locus_tag="VW077_001163"
     CDS             12498..13478
                     /gene="folP"
                     /locus_tag="VW077_001163"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9455186.1"
                     /translation="MVKSLEGVTDMSTKVNHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYSYVEIEEEIQRVVPVIKAIRKESDVLISI
                     DTWKSQVAEAALAAGANLVNDITGLMGDEKMAEVVAKAGAKVVIMFNPVIARPQHPSS
                     LIFPHFGFGQAFTEEELANFEKLPIEDLMETFFERALARANQAGIAQENILLDLGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHKMAVEIASAIRLADEAENLDLKQYK"
     gene            13480..14802
                     /locus_tag="VW077_001164"
     CDS             13480..14802
                     /locus_tag="VW077_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9455187.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVIHI
                     GGTNGKGSTIAFLKNMLEKLGLRVGVFSSPYLIHYTDQISINGESIPEARLEALMVDY
                     QSLLEGGVASNFQETTEFEIITAIAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGKDYQVSHQKSVVTGEVFDYTSAVRQGRFQTGLLGLHQIENAGIAIALLDTFCQE
                     DGRELASNHLLAQALEETSWPGRLEVVSRDSLMILDGAHNPHAIKALLATLQERFADY
                     RKEILFTCIKTKALEDMLDLLEEIPDTELTLTHFDDSRATDESVLKEAAKSRNLSYQG
                     WQDFLEQKLTDKKEEKKTVRIVTGSLYFLSQVRADLMERKNENGYTKD"
     gene            14783..15337
                     /gene="folE"
                     /locus_tag="VW077_001165"
     CDS             14783..15337
                     /gene="folE"
                     /locus_tag="VW077_001165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9455188.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            15380..>16187
                     /gene="folK"
                     /locus_tag="VW077_001166"
     CDS             15380..>16187
                     /gene="folK"
                     /locus_tag="VW077_001166"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9455189.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALK"
BASE COUNT         4689 a         3106 c         3690 g         4702 t
ORIGIN      
        1 tacaataaaa taggctccat aatatctata gtggatttac ccactacaaa tattatagag
       61 cctaaaaaat tgcttaataa tctattagaa gaggctaacg tatatccaat agatttacat
      121 tagctttctt ttttgttaaa atagtctatg gaaagagggt gagagtatgt caaagatgag
      181 tatgagcatc cgtctggata gtgaggttaa ggagcaggcc caacaggtgt ttagtaatct
      241 gggaatggat atgacaacag ctattaatat tttccttcgt caggcaattc aatatcaggg
      301 attacctttt gatgttagac tagacgaaaa tcggaagttg ctccaagtgt taacggattt
      361 agaccaaaat cgtaatatga gccagtcttt tgaatcagtc tcagatttga tggaggactt
      421 acgtgcttaa gattcgttat cataaacagt ttaaaaaaga ttttaagttg gctatgaagc
      481 gtggtttgaa ggcagaatta ttagaagaag ttttgaattt tctggttcaa gaaaaagaac
      541 atcctgccag atatcgtgat cattcattga cggcatccaa gcattttcaa ggagttcgtg
      601 aatgccatac ccagccagat tggcttttgg tttataaagt agataagtcg gaattgattt
      661 taaatttgct gaggacaggc agtcacagtg atttatttta atctatttta agggggttct
      721 catgaaacta agaatatttg cggaagataa gccggctaag aaggtatttg aatatcaatt
      781 agaacttgct gatcgtacaa ttcttctatc gacagcactc ttgtcaggtg ctattgcttt
      841 agcaggaatc ttttctgctt tgaaagaaaa ataaaaatag aaaagagaaa acaggatggt
      901 tttaccaaat tttaaagaaa atctagaaaa atatgcgaaa ttgttggttg cgaacggaat
      961 taacgtgcaa cctggtcaca ctttggctct ctctattgat gtggagcaac gtgaattggc
     1021 acatctaatc gtgaaagaag cttatgcctt gggtgcgcat gaggtcatcg ttcagtggac
     1081 agatgatgtg attaaccgtg agaaattcct ccatgccccg atggagcgtt tggacaatgt
     1141 gccagaatac aagattgctg agatgaacta tctcttggag aacaaggcta gccgtcttgg
     1201 agttcgttca tctgatccag gtgcctggaa cggagtggac gctgacaagc tttcagcttc
     1261 tgctaaagct atgggacttg ccatgaagcc aatgcgaatc gcaactcaat ctaacaaagt
     1321 tagctggact gtagcagctg cagcaggact tgagtgggct aagaaagtct tcccaaatgc
     1381 tgcgagcgac gaagaagcag ttgatttcct ttgggaccaa attttcaaaa cttgccgtgt
     1441 ctacgaagca gatcctgtta aggcttggga ggaacatgca gccattctca agagcaaggc
     1501 cgatatgctt aataaggagc aattttcagc ccttcactac acagcgccag gaacagattt
     1561 aacacttggt ttgccaaaga accacgtttg ggaatcagct ggtgctgtca atgcacaggg
     1621 cgaagaattc ttgccaaata tgccgacaga agaggtcttc acagcgcctg acttccgtcg
     1681 tgcagatggt tatgtcactt ctacaaaacc gcttagctac aacggaaata tcattgaagg
     1741 cattaaggtg acctttaagg atggacaaat cgtagatatc actgctgaga agggtgatca
     1801 ggttatgaaa gaccttgtct ttgaaaatgc gggtgcgcgt gccttgggtg aatgtgcctt
     1861 ggtaccagat ccaagtccaa tttctcagtc aggcattacc ttctttaaca cccttttcga
     1921 tgaaaatgcg tcaaaccact tggctatcgg tgcagcctat gcgactagcg ttgttgatgg
     1981 agcggagatg agcgaagagg agcttgaagc tgcagggctt aaccgttcag atgttcacgt
     2041 agactttatg attggttcta accaaatgga tatcgatggt attcgtgagg atggaacgcg
     2101 agtacctctt ttccgtaatg ggaattgggc aaattaagga gataatatgt taggaagtat
     2161 gttcgttggt ctcctagtgg gatttttagc aggtgctatg accaatcgtg gagagcgaat
     2221 gggatgtttt ggaaaaatgt ttctcggttg gatcggagcc tttctaggtc acttgctctt
     2281 tggaacttgg gggccagttt tatcaggaac agctattatc ccagctgttt taggtgccat
     2341 gattgtctta gctatttttt ggagacgagg aagttaattt cttaaatctg atactcaatg
     2401 aaaatcaaag agcaaactag gaaactagcc gcaggttgct caaagcactg ctttgaggtt
     2461 gcagatagaa ctgacgaagt cagtaacata tatacggcaa ggcgacgttg acgcggtttg
     2521 aagagatttt tgaagagtat gaaacgaaaa ggaggttggt cattgtacca gcctcctttt
     2581 gatatgatat aatagttcta tgagattaga taaattttta gttgcctgtg cggtgggaag
     2641 tcggactgag gtcaaaaact tgctcaaggc tgggcgcgtg actgtaaatg gtaaaaaaga
     2701 aaaatcagct aaattgcaga ttgatgaaaa aatagatgag attcgctttg atgggcaagt
     2761 gttggagtat gaagagtttg tctactacat gatgaacaag cccaaaggag ttatatcagc
     2821 gactgaggat cccaagcaca gaaccgttct ggacttgttg gatgacttgg cgcggagtaa
     2881 ggaaattttc ccagtaggac gcttggatat tgacacgcat ggtcttttgc tcttgaccaa
     2941 tgatggtcag ctggctcatg ttcttctttc gcccaagcgt catgtggaca agacttatct
     3001 ggcacaagtc aagggaatca tgacccaaga agatgtggag atatttgctg agggtattcc
     3061 tctcaaagac tttacctgtc aacccgctat actggagctt gtatccatag atacagaaaa
     3121 gaatcaaagc caaatccgtg tgaccattgc agaagggaag tttcatcaga tcaagcgtat
     3181 ggtgggctac tgtggcaagg aagtggtgga cttgcaacga ttgactatgg gaactttagt
     3241 attagatgag aacctggaac gaggggaatg gcgtcgcttg accaaggaag aattagaaat
     3301 tctccttgct agtattgctt aatttgattt atattcgaaa aaggtgaggg agaatgtcct
     3361 taccttttat gttttttagt tgcttccttt gtgaaaagag ttataataga ctgtagaata
     3421 aaaggaggaa tctatgaacg cgattcaaga atcatttact gataaactat ttgccaacta
     3481 tgaagcaaat gtaaaatacc aagcgattga aaatgctgct agccacaacg gaatttttgc
     3541 agctctggaa cgtcgccaaa gccatgtaga caacacacct gttttctcat tggatttaac
     3601 caaggacaag gtcactaacc agaaagcgtc tggtcgttgc tggatgtttg cggctctcaa
     3661 caccttccgc cacaaactca tctcgcaata caaattggag aactttgagt tgtcacaagc
     3721 ccacactttc ttctgggata agtatgagaa atcaaactgg ttcttggagc aagtcattgc
     3781 gacttcagac caagaattga ctagccgcaa ggttagcttc ttacttcaaa cacctcaaca
     3841 agatggtggt caatgggata tggtcgtttc cctctttgaa aaatacggtg tcgtgcctaa
     3901 gtcagtttat cctgagtctg tttcatctag cagcagtcgt gagctaaatg cgatccttaa
     3961 taaattgctt cgtcaagatg ctcaaatctt gcgtgacttg cttgtttctg gtgcagatca
     4021 agcgactgtt caagctaaga aagaagacct cttgcaagaa atctttaact ttcttgctat
     4081 gtcattagga cttccaccac gcaagtttga ctttgcttat cgcgataaag ataacaacta
     4141 caaaagtgaa aaaggaatca caccacaaga gttttacaag aaatatgtca atcttccttt
     4201 agaagactac gtttctgtta tcaatgctcc aactgctgat aaaccttacg gaaaatctta
     4261 cacagttgag atgttgggga atgtggttgg tagccgtgca gttcgttaca tcaacgttcc
     4321 aatggagcgc ttgaaagaat tggcgattgc ccaaatgcaa gcaggtgaga ctgtttggtt
     4381 tggttctgat gtcggccagc tcagtaaccg taaggctggc atccttgcga cagatgttta
     4441 tgactttgaa tcaagcatgg acattaaact tactcaagac aaggctggac gtttggacta
     4501 tagcgaaagc ttgatgaccc acgccatggt cttgacaggt gttgacttgg acgaaaatgg
     4561 taaatcaacc aagtggaagg ttgaaaactc atggggagac aaggtcggta cagatggtta
     4621 ctttgttgcc tcagacgctt ggatggacga atacacatac caaatcgttg ttcgtaagga
     4681 attgctgaca gcagaagaac aagctgccta tggagcagaa ccaattgtac ttgcaccatg
     4741 ggatccaatg ggagccttgg ctgaataaaa gcatagaaaa aaggaatcag attttagaac
     4801 ctggttcctt tttagttgct tgattacatg atgtgaagaa catgtgccac aatacccact
     4861 gcgaagaggg caaggataat agtgattgga gatacttttt tcttaagtaa gtacatgcaa
     4921 agtaaagtaa ggagtagtcc tgataatcca ggaatcaaca tatccaagtt ttgttggaaa
     4981 gtagtaactt tttcaggagt ttgagacaat ccttgtccta cttgtgcgaa tgcttcttgg
     5041 atacctttag acccttctgg caatttatcc caatggatat aagccttttc atctagttga
     5101 actttagaaa catcgaaagc aaatttaata tttacccagc gttgaacaag gacagcaaga
     5161 atgaacatcc caaggataga agctccttta gtgatatctt gaaggatacc accagacata
     5221 tctttagtga tttctgatcc agccttgtat ccaatctctt gaacatacca caagaatgac
     5281 atacgaatca agttccatgc aacaaagaag aggagtggac ccaagatatt gccagtaagg
     5341 gcaagtgaag caccgagaga tccaaggatt gggcgcactg taaaccagaa tactgggtca
     5401 ccgataccag caagaggtcc catcataccg attttaaccc cttggatagc agcgtcatcg
     5461 atttccacac cgttagcacg ttcttcttca agcgcaagag taacccccat gactggagca
     5521 gctacgtatg gatgagtgtt gaagaactca aggtgacgct caagagcagc gatttgatct
     5581 tcttttttag tatagagttt tttgatagct ggaatgagtg tataagccca gcccaagttt
     5641 tgcatccgtt caaagttcca agacccttgt aagaaggttg aacgccacca aactttttta
     5701 cgatctgatt tagttaattg aagtttttca gtcatgatgt tttcagtcct ttcttatctt
     5761 agtagtcttc taggatgtcg ccgattgggt cgttagaagt tgcggctcct ccgccaccat
     5821 ttccaccagt tttagaaagg tgaaggtaga taagagcgat agcaacaccg atagcaccga
     5881 atccgattag agtaatatct gacacagcag cgagaacgaa accaagagcg aagaatggcc
     5941 atacttcacg agttgccatc atgttgataa ccatagcgta accaacggca acgaccatac
     6001 caccaccgat agccatacca tctttgagcc agtctggcat ggcactaagg atactttgta
     6061 cagtttcagt tggtaccata aggagaagag ctgcaggaag cgcgatacga agtccttgga
     6121 agagtagcgc aatgaaatgc gcacgctcca cagcgccgaa gtcacctttt ttagcggcag
     6181 catctgcagt gtgaaccaaa ccaactgaaa ttgtacgaac aatcattgtc aagaaaagtc
     6241 cagctacggc aagagggata gcaaccgctt gggcaacacc gataccagtc ttggtaaagt
     6301 caccaccaag aaccatgata atggcagcag cgacagaagc aagtgcagca tcaggagcga
     6361 tagcagcacc gatatttgac caaccaaggg caatcatttg aagcgatcca ccgaggataa
     6421 tccctgcttc caagtgacct gttacaagcc caataagggt acaggctaca agtggttggt
     6481 gaaattggaa ctggtcgagg atgccttcaa gacctgcaaa gaaggctaca acgactacta
     6541 aaaccataga aataatagac atgtttaaaa tcctttcata aataatggct tatttgacat
     6601 tggctttgtt aatcaagtca aacaaatctt ttttagaatc atttggtact ttacggacat
     6661 caaattcaac acccaagtca cgcatttttt caaatgtagc aacatcttct ttgtccatag
     6721 acaaaacggt attgaccaat gttttacctg ttgagtgagc catagaacca acgttaagag
     6781 tcttgattgg cacgccgcct tcgatggcac gaagggcatc ttgaggtgtt tcaaacaaga
     6841 taagggcatg tgtttctcca aaacgtgggt cttttgaaat ctcaatcagt ttttgaattg
     6901 gaaccacgtt agccttgaca ttacctggag ctgcttgttt aatcaattct ttacgaaggt
     6961 cgtctttagc cacgttatct gaagcaacga tgatacgatt tgcttttgaa tctggagtcc
     7021 aagcagttgc aacctgaccg tgaagtagac gtgtgtcaag acgggcaaga ttgattttca
     7081 atttaccgtc tccgataaca gttccttctg ggatagcagt ttgggcaact ggagcagctg
     7141 cagcgcttgc aacctcttca actggattta gctcttctgg aagagctttg atgccatctt
     7201 tggcttcttt aatgatatta gcagcgactt tttctacacc tgcagcagcg tccatgaggc
     7261 gctctgtgta ggcttgaatc aacatcggta agttaagtcc tgtgatgatg gcaaacttac
     7321 gctcaggatt ttctcccatc acgcgactag cttggttaaa tggagaacca ctccaaaggt
     7381 cagccaaaac tagaacctca tcttctgcgt caaatgcagc aacagcgtta ttaaacttag
     7441 cgtatagatc atcaggacct tcatttggca taaaggtcac aacttgaacc ttttcttgtt
     7501 caccaaagat catagatcct gactgatgaa tacccgcagc aaattcgccg tggctcgcaa
     7561 taatgattcc gatactcatt attgtcattc ctcctttttt gttttagttt tcatttaaga
     7621 aaactttaag actgtttaag tataaaccgt tttcataaaa aaagcaacta tttatcataa
     7681 aaaattaaaa aaattttatt tttgtatgtt gatatgttga gtttttgagg agtaatcttg
     7741 aattcttatt taaaaattta atataaaaaa gttggaagaa gcttccaact tttaaaatag
     7801 gtttctatta gattagtggg tgaagtcgag taccatacgt ccttggattt gacctttttc
     7861 catttcgtcg aaaatggcaa cagcatcttc tactggacgt ttttgaacaa ctggggctac
     7921 cagaccttct gcaccaaatt ggaaggcttc ttctaagtct ttacgagttc cgacaagaga
     7981 accgatgact tggattccat cgaggactgt tttaacgatg ctgagttcca tcatttcaga
     8041 aggaagacca acagcgacga cgcgaccacc agcacgaacg gagtcaacag cctggttgaa
     8101 ggcaacttta gacacagcag ttacgacagc tgaatgagct cctccatcag ttttttcttt
     8161 gatgagtcca gctacatctt caacttcgag gccgttaatc acaatgtcag cgcctacttc
     8221 ttttgcaagg gcaagtttgt cattgttgat atcgactgcg ataacatgag cattgaatac
     8281 ttttttagcg tattgaacag cgaggttacc aagtccacca gcaccgtaaa gaacaaccca
     8341 ttggcctggt tcaacttttg cttctttgat agctttatag gttgttactc cagcacatgt
     8401 gatagaagaa gcttgggctg gatcaagtcc gtcaggaact ttgacagcat agtcagcagt
     8461 tacgatacat tgttcagcca taccaccgtc tactgagtag ccagcatttt tcactgtacg
     8521 gcaaagggtt tcgcgaccag ttgtacagta ttcgcaagtg ccacatcctt caaagaacca
     8581 agcaacgctg acgcggtcac cgactttaag gcttttcaca tctggagcaa tctctttaac
     8641 gataccgata ccttcgtgcc caagaacacg tcctgggact tgaccaaagt caccatgagc
     8701 aacgtggagg tcggtgtggc aaacgccaca gtattcaact tctacaagtg cttccccagt
     8761 ttcaagtgga cggagtactt tttcttcaat agcaacacca gtgctttctg gatttacaac
     8821 aacagctttc atagctatcc tccttgatat tgaccgagaa cagggacaac gggactggat
     8881 tgattttttg tcaacagagt cgagtgtgac gagctctctt gtatttatat gtgaagtata
     8941 tcacaaaaga aagccttttc caaggttttg gagacaaaaa gtagaacaat cgattaactg
     9001 gttgatgaga ctgtgaaaag agcacaaaga ccagcttgca agctggtcat actcttcgaa
     9061 aatctcttca aaccacgtca gcttcatcta caacctcaaa gcagtgcttt gagcaaccta
     9121 cggctagctt cctagtttgc tctttgattc tcattgagta tcagtgtagg ctataagaat
     9181 aagtcttgta gggttttggc aaccccatct tggtcgttgg taagggaaat ttgctcatct
     9241 gcataaggga gtagctctgg attggcattt ttcatggcat aacctttccc agcaaaagcg
     9301 agcatttcgg tatcattgtg ttcatctcca aaggcaatca aatctttttt gtcacgattc
     9361 attatcttga gcaagtagtc caaagcaaag gccttgttga caccttttgg ggtacattca
     9421 aggatattga gcggacctcc ccaggtattg atagaaagtt gatgctggta gaaggcgttc
     9481 atgtcttttg ccaaggcata tttgtcactg gctctggtct gcaagaggat acagttaggg
     9541 tccttggtca ccaattcagg ctggaattga tcttcaggct ggaaagcttc tacaccaaat
     9601 agtttgggat tggcaatttc ttcattggga tttgtaatgt aaaatttttt acgatattct
     9661 ccagcgataa aatcggcttg aatgtcctct gaacgttgaa ccatatctag cagatatttt
     9721 ttgtctacag tcaaacactt ttcaaaatcc caaacttggt ctggtaaatg agtaagggat
     9781 ccgttgaagt taatcatagg agtgtctaag cccagttcac ggtaaaaatc ttttgacata
     9841 cggtaagggc gacctgtcgt aataataacc tgatggcctt tttcagcaac ttttttgatg
     9901 gtttttttgg taaagtcaga aatttgactg tctgagttga gcagggttcc atccaggtca
     9961 actgcaataa tttttttcgt catatggacc tttctagtgt gttttcagat aagatgtctt
    10021 ctattataac agaaagcagg cagaaaagca gattccaaac aaaaaatgaa attggattaa
    10081 tttcttaaac aaatcaaaca aagacattga aaaagtgaat gataaatgat ataattctat
    10141 tattgttcgt aaaaattaaa aggagattga tgatggacaa attatttaaa ctaaaagaga
    10201 acggtacaga cgttcgtaca gaggttctcg ctggtttaac aactttcttt gcaatgagtt
    10261 atattctctt tgtaaaccca caaattcttt cgcaaacagg aatgcctgct cagggtgtat
    10321 tcctcgcaac gattatcggt gcagttgctg gtaccctgat gatggctttt tatgctaact
    10381 taccttatgc ccaagcgcca ggtatgggac tcaatgcctt ctttaccttt acagttgtat
    10441 tcgggcttgg ttattcttgg caagaagccc tagctatggt cttcatctgt ggaattatct
    10501 cattgattat taccttgaca aatgttcgta aaatgatcat tgaatcgatt ccaaatgctc
    10561 ttcgctcagc tatttcagct ggtatcggtg tctttcttgc ctatgtaggg attaagaatg
    10621 ctggactttt gaaattcacg attgatccag gcaactatac tgttgtagga gaaggggcag
    10681 acaaagctca agcaacgatt gcagcaaact cttcagcagt tccaggattg gtcagcttta
    10741 ataatccagc tgttttagtg gctcttgcag gacttgctat cacaattttc tttgttatca
    10801 aaggaattaa agggggaatt atcctctcta tcttgacaac aactgttctt gctatcgcag
    10861 ttggtttggt agatttgtct agtatcgatt ttgctaataa ccatgttggt gcagcttttg
    10921 aagatttgaa gacaatcttt ggtgcagctc ttggttcaga aggtttggga gctttggttt
    10981 cagatacagc tcgcttgcct gaaactctga tggccattct tgccttctca ttgacagata
    11041 tttttgatac aattggtact ttgattggta caggtgaaaa agttggtatc gtagcgacaa
    11101 atggtgaaaa tcaccaatca gccaaattgg acaaggctct ttactctgat ttgattggaa
    11161 cgacagtcgg tgccatcgca ggtacttcaa atgtaacgac ttatgtcgag tctgctgctg
    11221 gtattggtgc aggtggacgt actggtttga cagccttggt tgtagctatc tgttttgcga
    11281 tttcaagttt ctttagccca cttctagcga ttgtaccaac agccgctaca gctccaatct
    11341 tgattatcgt tgggattatg atgttggcta gcttgaaaaa tatccattgg gacgatatgt
    11401 ctgaagcggt tcctgctttc ttcacatcta tctttatggg attcagctac tctatcactc
    11461 aagggattgc agttggtttc ttgacttaca ctttgactaa acttgtcaaa ggtcaagcta
    11521 aagatgttca tgtcatgatt tggattttgg atgccttgtt tatccttaac tacatcagca
    11581 tggccctata aatgctataa tataaaaagg ggttctcccc tttttatatt atagttactt
    11641 gaaaattgct tcaaactagg taggtgattt tgattgaatg gtaggagtat aattgatgat
    11701 aaaaaagaat atttgggatg aagtattaaa cagggggaag tggttaataa tctttctagt
    11761 agggcttttg ttttctcagt ttcctttagc tctagcaact tttctaacta gcagaaaatt
    11821 tccactgctt caaactggac tattagtagg gaccttatct cttgtggttt taactgtatt
    11881 tataataggt gctaagaaaa cacagttggc tagttttggt ttatcgtttt tcaaggctaa
    11941 ggatttggct agattggcct taagctatct agtgattctt gcctttaata ttcttggtgt
    12001 ggtcttactt catttgatga atgaaacaac aaccagcaat caatcaaata ttaatgattt
    12061 ggtccagaat agttccttaa tttccagttt tttcttactg gttctaatag ctcctatttg
    12121 tgaggagatt ttatgtcgag gagtgattcc taaaaaactc tttcgtggca aagaaaatgt
    12181 gggttatata gtcggtgcta ttatctttgc cttgcttcat ttaccgacaa atctaccttc
    12241 tcttcttatc tatggaggaa tgtcaactgt gctaacttgg acagtttata aaacccaacg
    12301 tttggaaatg tctatcttac ttcacatgat tgttaatgga atttttttct gtttgttggc
    12361 tctcgtggtg attttgagtc ggacattagg gatttctgtt taagattttt gacaattgct
    12421 tacttgtttc tattgggaaa aaagatgaat gcaatagtgt ccatcttttt ctttttatgg
    12481 taaaatagag aaataatatg gtgaaaagcc ttgagggagt gaccgatatg tcaactaaag
    12541 taaatcatgc aaagacagct atttgcggaa ttatcaatgt aaccccagat tccttttcgg
    12601 atggtggtca attttttgct cttgagcagg cactccagca ggctcgtaaa ttgatagcag
    12661 aaggggctag tatgctagat atcggcggag aatcgactcg gccgggaagt agctatagct
    12721 atgttgagat agaagaggaa atccagcgtg ttgttccagt gattaaagcg attcgcaagg
    12781 aaagtgatgt cctcatctct attgatactt ggaagagtca ggtagcagaa gctgctttgg
    12841 ctgctggtgc caatctagtt aatgatatca ctggtcttat gggtgatgag aaaatggcgg
    12901 aggtggttgc taaggctggt gcgaaagtgg tcatcatgtt taatccagtt attgctcgac
    12961 ctcagcatcc tagctcgctc atctttcctc attttggttt tggacaagct tttacagagg
    13021 aagagttagc taactttgaa aaattgccaa tcgaagactt gatggagact ttctttgaac
    13081 gtgcactagc gagagcaaat caagctggaa ttgcacaaga aaatatcctg ttggatctag
    13141 gaattggctt tggtctgacc aagaaagaaa atttgcttct tttacgggat ttggataaac
    13201 tacatcagaa gggctatcca atctttcttg gagtgtcgcg taagcggttt gtcatcaata
    13261 ttcttgaaga aaatggtttt gaagtcaatc ctgagacaga acttggtttc cgcaatcgag
    13321 atacggcttc ggctcatgta accagtatcg ctgcaagaca gggtgtagaa gtggtgcgcg
    13381 tgcatgacgt agccagtcac aagatggcag ttgaaattgc atcagccatt cgattggctg
    13441 atgaagcgga aaatttagat ttaaaacaat ataaataaga tgaaagaaat tgaaaacaat
    13501 cagtggattg ctaactaccg gacggatcaa ccgcattttg gcttggaacg aatggtggaa
    13561 ctgttagctt tgcgcggaaa tccccatctc aaactcaaag tcatccatat cggaggaacc
    13621 aatggcaagg gttcgaccat tgcttttttg aaaaatatgc tagaaaaact agggctgaga
    13681 gttggtgttt tcagctcgcc ctatctcatt cattatacag accagattag catcaatggg
    13741 gaatctatcc cggaggctag actagaagcc ctcatggtag actatcagtc tttgctggag
    13801 gggggagtgg cttccaattt tcaggagaca actgagtttg agattatcac agccatagcc
    13861 tatgactact ttgcctcaga gcaagtagat gtggccatca tggaagtggg tatgggggga
    13921 cttttagata gtaccaatgt ctgtcagccc attttgacag gcattacgac tattggactg
    13981 gaccatgtag ccctacttgg tgacaccttg gaggccatag cagagcagaa ggcaggtatt
    14041 atcaaacaag ggatgccctt ggtgacaggt cgtatcgctc cagaagcttt ggctgtgatt
    14101 gaccgcattg cggaagggaa agatgcgccg agacttgcct atgggaaaga ttatcaggtc
    14161 agccatcaaa agagtgtggt gacaggagag gtctttgatt atactagtgc tgtcagacaa
    14221 gggcgcttcc agacaggttt acttggtttg caccagatag agaatgctgg gatagccata
    14281 gctttacttg atactttttg tcaagaagat ggtcgagagc tagcaagcaa tcatttgctt
    14341 gctcaagctc tggaagaaac aagttggcca ggacgtttgg aagttgtgtc gagagattcc
    14401 ttgatgattt tagatggagc ccacaatccc catgctatca aggctttatt agcaaccttg
    14461 caagaacgct ttgcggatta tcgtaaggaa atcctcttta cttgtatcaa aaccaaggcc
    14521 ttggaggata tgttggactt gctggaggaa atcccagata ccgagcttac tctgacacat
    14581 tttgacgata gtcgggcgac tgatgaaagc gtgctgaaag aggcagctaa gtctagaaat
    14641 ctcagctacc aaggttggca ggattttcta gagcagaaat tgacagataa aaaagaagag
    14701 aaaaaaacag ttaggattgt cacgggttcc ttgtatttct tgagccaagt gagagccgat
    14761 ctgatggaga ggaagaatga aaatggatac acaaaagatt gaagcagctg taaaaatgat
    14821 tatcgaggct gtaggagaga acgctaatcg cgagggcttg caggaaacac ctgctcgtgt
    14881 agcccgtatg tatcaagaga ttttttcagg tcttggtcaa acagcagagg aacatttgtc
    14941 aaaatccttt gaaattattg acgataatat ggtggtagaa aaggatatct ttttccatac
    15001 catgtgtgaa caccacttct tgccatttta tggtagagcg cacattgcct acattccaga
    15061 tggtcgtgtg gcaggcttgt ctaagctagc ccgtacggtt gaagtttatt cgaaaaaacc
    15121 acaaattcaa gaacgtttga atatcgaagt ggccgatgcc ttgatggact atctaggtgc
    15181 taaaggagcc tttgttgtca ttgaggcgga acatatgtgt atgagtatgc gtggtgttag
    15241 aaaaccaggc actgcaacct tgacgacagt agctcgtggt ctatttgaaa cagataagga
    15301 tctccgtgac caagcttatc gtttaatggg gctataaaaa gaatccgctt caagcggatt
    15361 tttctagaaa ggaatcatta tggatcaact gcagattaag gatttggaaa tgtttgccta
    15421 tcatggtctt tttcctagtg agaaagaatt ggggcagaaa tttgtcgttt cagccatcct
    15481 atcctatgat atgaccaagg cagctacaga cttggattta acagcctctg tccattacgg
    15541 agaattgtgt cagcagtgga cgacttggtt tcaggaaacg agtgaagatt tgattgaaac
    15601 ggtagcctat aaactggtgg aacgtacctt tgagtcttat cctcttgtcc aagaaatgaa
    15661 gttggaactg aaaaaacctt gggcaccggt gcatttgtca ctagatactt gctcggtaac
    15721 cattcatcgc cgcaagcaac gagcctttat cgccctagga agcaatatgg gagataaaca
    15781 agcaaacttg aagcaagcca ttgacaaact gcgagctcgt ggcatccata ttctcaaaga
    15841 gtccagtgtc ttagcgacgg agccttgggg tggagtggag caggatagct ttgccaatca
    15901 agtggttgag gtggaaacct ggctaccagc acaagacttg ttagaaacct tgttagccat
    15961 tgagtcagag ctgggacggg tgagagaagt gcattgggga cctcgtttga ttgatttgga
    16021 cttgctcttt gtggaggacc agatccttta tacagacgac ctcatattgc ctcatcctta
    16081 catagcggaa cgcctttttg tccttgagtc cttacaggaa attgcgcctc attttatcca
    16141 tccgatatta aaacaaccga tccgcaactt gtatgatgct ttgaaaa
//
LOCUS       DAVKGU010000038        16119 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKGU010000038 DAVKGU010000000
VERSION     DAVKGU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16119
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.038"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            199..852
                     /locus_tag="VW077_001167"
     CDS             199..852
                     /locus_tag="VW077_001167"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9455190.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            862..1326
                     /locus_tag="VW077_001168"
     CDS             862..1326
                     /locus_tag="VW077_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9455191.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECATREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1339..2607
                     /locus_tag="VW077_001169"
     CDS             1339..2607
                     /locus_tag="VW077_001169"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9455192.1"
                     /translation="MLLIKNGRVMDPKSGLDQICDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2621..2881
                     /locus_tag="VW077_001170"
     CDS             2621..2881
                     /locus_tag="VW077_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455193.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2892..4253
                     /gene="pdrM"
                     /locus_tag="VW077_001171"
     CDS             2892..4253
                     /gene="pdrM"
                     /locus_tag="VW077_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9455194.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSPLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            4407..5375
                     /locus_tag="VW077_001172"
     CDS             4407..5375
                     /locus_tag="VW077_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9455195.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5391..6383
                     /locus_tag="VW077_001173"
     CDS             5391..6383
                     /locus_tag="VW077_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9455196.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6601..7644
                     /locus_tag="VW077_001174"
     CDS             6601..7644
                     /locus_tag="VW077_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9455197.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7708..9393
                     /gene="lpdA"
                     /locus_tag="VW077_001175"
     CDS             7708..9393
                     /gene="lpdA"
                     /locus_tag="VW077_001175"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9455198.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEEKGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9457..10446
                     /locus_tag="VW077_001176"
     CDS             9457..10446
                     /locus_tag="VW077_001176"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9455199.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10517..11587)
                     /gene="xerS"
                     /locus_tag="VW077_001177"
     CDS             complement(10517..11587)
                     /gene="xerS"
                     /locus_tag="VW077_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9455200.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSALENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(12162..13712)
                     /locus_tag="VW077_001178"
     CDS             complement(12162..13712)
                     /locus_tag="VW077_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9455201.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMSDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQILGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(13728..14507)
                     /locus_tag="VW077_001179"
     CDS             complement(13728..14507)
                     /locus_tag="VW077_001179"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9455202.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(14494..15345)
                     /gene="ylqF"
                     /locus_tag="VW077_001180"
     CDS             complement(14494..15345)
                     /gene="ylqF"
                     /locus_tag="VW077_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9455203.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(15819..>16119)
                     /locus_tag="VW077_001181"
     CDS             complement(15819..>16119)
                     /locus_tag="VW077_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9455204.1"
                     /translation="LIKNEVRMMMSCIAYNLYLFLKHLSGGDFQTLTIKRFRHLFLHV
                     VGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT         4776 a         3100 c         3464 g         4779 t
ORIGIN      
        1 aagataatct tcgaaagagg tagtaaaatg cttaatgatt taaaaagtaa ttataaaaaa
       61 tagccagtct ttataaactg tattcacgta ttcaaatcgt atatagtcga tttattaaat
      121 ctttagataa attgtaaaaa gtggtttcca tagccacttt ttgctataat agaggcagta
      181 aaaatgaagg agtcagctat ggaacactcg tcttggcatg ctttgattaa ggcgcaatta
      241 cctgagggtt atttcgggaa aatcaatcag tttatggagc aggtctattc tcaggggatt
      301 atttatccac ccaaggaaaa ggtttttcag gctctcttga caacactgct tgaagaagtt
      361 aaggtggtaa ttctagggca agacccctat cacggaccag gtcaagcgca gggcttgagt
      421 ttttctgtac ctgactctat cccagctcca ccatccttgc aaaatatctt gaaagaattg
      481 tcagatgata tcggagttaa gaaatctcat gatttgacag cttgggctga gcaaggagtc
      541 ttgcttctta atgcttgttt gactgttcct gctggacagg ccaatggtca tgctggtcaa
      601 atctgggagc cttttactga tgctgtgatt caggtggtca atcatctaga tagaccagtc
      661 gtttttgtac tctggggagc ttatgcacgt aagaagaagg ccttagttac caatcctcat
      721 cacttgatta tcgaatcagc ccatccaagt cctttgtcgg tttatagagg attttggggt
      781 tccaagccct tttccaaggc caatgcattc ttaaaagaga caggacaaga gccaatcgat
      841 tggcttagat aaggagaaaa tatgcctcag ttagcgacga tttgctacat tgataatggg
      901 aaagaactgc tcatgctcca tcgtaataag aaacccaatg atgtccatga agggaaatgg
      961 attggtgtgg gtggtaagct agagagagga gagacgcccc aggaatgcgc gacgcgtgaa
     1021 atccttgaag aaacagggct caaagccaag ccagttctaa aaggtgtcat cacttttcct
     1081 gaatttacac cagatttaga ctggtacacc tatgttttta aggtgacgga gtttgagggc
     1141 gacttgattg actgcaatga ggggacgcta gaatgggttc cctatgatga ggttttgagc
     1201 aagccgactt gggaaggtga ccacaccttt gttgagtggc ttttagagga taaacccttc
     1261 ttttcagcca agtttgttta tgatggggat aaattgttgg atacccaagt tgatttctat
     1321 gaataaagga gaaagcagat gctactaatc aaaaatggtc gtgtaatgga tcccaagtct
     1381 ggtttggatc aaatttgtga tgtcttagtt caagatggga aaattatcaa aattgcgcct
     1441 gagatcacgg aagaaggagc agaaacgatt gatgctactg gtcttgtggt tgctcctggt
     1501 ttggtcgata ttcatgtgca tttccgtgaa cctggtcaaa cacataaaga agacattcat
     1561 actggtgccc tagcagccgc tgcaggtggt tttactactg tcgtcatgat ggctaatacc
     1621 agtccaacca tttcagacgt ggagactttg caagcagttc tccagtcagc tgccaaagag
     1681 aagattaatg tcaagacagt tgcgaccatt actaaaaact ttaatggtaa aaacttgact
     1741 gactttaagg cactcttaga agctggtgcg gttggtttct ctgatgacgg tattccgctt
     1801 gagagcagta agattgtcaa ggaagccatg gaggaagcca aaaaactcaa tacctttatc
     1861 agccttcatg aggaagatcc aggtttgaac ggtgttcttg gctttaatga aaatattgct
     1921 agagaacatt tccatatctg cggtgctact ggggtggctg agtacgctat gatggcgcgt
     1981 gatgtcatga ttgcctatgc aactaaagcc catgttcaca tccagcattt gtctaaggaa
     2041 gaaagtgtta aagtagtgga gtttgctcag gggttaggtg cagaagtcac agcagaagta
     2101 gcgccacagc atttctctaa gacagaagca cttcttttaa cacaaggtag caatgctaag
     2161 atgaatccac cgcttcgttt ggaatcagac cgtcgtgccg ttatcgaagg tctcaaatca
     2221 ggtgtcatca cagttattgc gactgaccac gcgcctcatc atgtagatga aaaaaatgtt
     2281 gaggatatta ccaaagcgcc atctggtatg actggcttag aaacatccct gtctctcagc
     2341 ttgacctatt tagtagaagc tggtgagttg agcttgatgg aattacttga aaaaatgaca
     2401 tacaacccag ccaagcttta caactttgaa gcaggttact tggctgagaa tggtccagca
     2461 gatatcacta tttttgatgc caaggctgac cgctttgtgg actcccattt tgcttccaaa
     2521 gcagctaatt caccattcat cggtgaaacc ttaaaagggc aggttaaata taccatctgt
     2581 aagggacaaa tcgtctatca agcttgatag gagttagaat atgaattatt ttcgaaaacg
     2641 tagggagaga caagcgaaat ctaatagtgg aatttactgt cccgcagcta atcgttccaa
     2701 aattcaatat gaaactaaag aaaaagctga ccatgcggtt caatatgata acggaggttt
     2761 agttagaagc tattattgtc gtacatgtgc gtgttggcat actacttcaa aatctaataa
     2821 acctccgcta agtttgaaaa actatggtct gaaactcttt ggtttagata aatcagaata
     2881 gaaagagaag tatgtataag acaaagtgtt tacgagagaa gttagtatta tttttaaaaa
     2941 ttttcttccc aatcctgatc taccaatttg ccaattattc tgcctctttt gttgatactg
     3001 caatgacagg tcaatacaac actatggact tggctggtgt atctatggca accagtatct
     3061 ggaatccttt ctttacattt ctaacaggga ttgtgtcagc cttggtgcct atcattggtc
     3121 accatcttgg tcgaggcaaa aaggaagaag ttgcgtctga tttttaccaa tttatttatt
     3181 tggccttggg cctatctgtg gtcttgctgg ggatggtact tttcttggca ccaataatct
     3241 tgaatcatat tgggttagaa gcagcagtag cggcagtagc ggttcgctat ctttggtttt
     3301 tatctatcgg gattatcccc ttgttgctct ttagcgtcat tcgttccttg ctggattcgc
     3361 tgggcttgac caaactgtcc atgtacctca tgcttttgtt actccctctc aatagcggat
     3421 ttaactatct cttgatttac ggtgcctttg gtgttccaga actgggaggg gctggtgctg
     3481 gtttaggaac atccttggcc tactgggtct tgcttgggat ttctgttctg gttttattta
     3541 aacaggagaa gctcaaagcc ttacaccttg agaaacgaat tccacttaat atggataaaa
     3601 ttaaggaagg agttcgttta ggtctgccta ttgggggaac tgtcttcgcg gaagtggcta
     3661 tcttttcagt ggttggcttg attatggcta agttttcgcc cttgattata gctagtcacc
     3721 agtcagctat gaacttttca agtcttatgt acgcctttcc tatgagtatc tcatcggcta
     3781 tggctattgt cgtttcctat gaagtgggag ccaagcgatt tgatgatgcg aaaacctata
     3841 ttggtctagg aagatggact gccctcattt ttgcggcctt caccttaacc ttcctttaca
     3901 tttttagggg aaatgtggcc agtctttatg gtaacgaccc aaaatttatc gatttgacag
     3961 tgcgtttttt aacttatagt cttttcttcc agttagcaga tacctttgcg gcgccgcttc
     4021 agggaatttt gcgggggtat aaggatacag ttattccttt ttaccttggt ttgcttggtt
     4081 attggggcgt agcaatccct gtggctacgc tatttgattc cctaacagat tttggagctt
     4141 attcttactg gatcggcttg attattagtt tgattgtgag cggggcgctc taccgttggc
     4201 gtttaactgt gattatgaag agatttgaat ctttagcaaa atccaaatgc taaaaagttt
     4261 gtgaaaaaat attcttgaca agaaaatccc ttgttttcac ggggttttct tttttatttt
     4321 gtgaaatttc ttttagaaaa actttgacag tatttgccaa aagcggtaaa atagtaaggt
     4381 aagaagaaag ttagaaaggc aagaagatgt caactttaga taaaaatctt ttgctagaaa
     4441 tgttccgtaa gatggaagaa atccgtcgca tggacttaaa aattgcacaa ttagtaaaga
     4501 aagggaaagt gccaggaatg acgcactttt ctgttggtga agaggcagct aacgtggggg
     4561 ctatgttagc tctcaatcca gatgatctga ttacctcaaa ccaccgtgga cacgggcaag
     4621 ctattgctaa agggattgac ctcaacggaa tgatggctga aatccttggt aaatacactg
     4681 gaacctgtaa agggaaaggt ggatctatgc atatcgctga ccttgatgct gggaaccttg
     4741 gtgccaatgg tatcgtaggt ggtggtatgg ggatcgctgt cggtgcagcc ctcagtcagc
     4801 aaatgcaaaa taccggtaaa atcgttgtct gcttctttgg agatggtgcg accaatgaag
     4861 gtgttttcca cgaagcagtg aacatggctt ctatctggaa cctgccagtc attttctatt
     4921 gcattaacaa cggttacggt atctctgcgg atatcaagaa aatgaccaat atagaacata
     4981 tccatcaacg tagcgccgct tatggaattc ctggaatgtt catcgaagac ggtaacaatg
     5041 tcatcgatgt ctatgaagga tttcagaaag ctgtggacca tgttcgcagt ggcaatggtc
     5101 cagtcttgat tgaaagtgta acttatcgct ggctcggtca ctcatcatct gaccctggta
     5161 aatatcgtac gcgtgaagaa gtggaattgt ggaaacaaaa agatccaatc gaaaacctcc
     5221 gcaattacct tattgaaaat aacattgcaa gtgccgaaga attggaagaa atccaagcgc
     5281 aagtaaagga agcagtagaa gcttctgtta aatttgcaga ggaaagccca ttcccaccgc
     5341 ttgaatcagc ctttgaagat atttacgcag actaaggaga aagagataaa atggaaacaa
     5401 aaacaatgtc cttccgtgac accattatcc ttgctatgtc tgaggaaatg cgtcgcgatg
     5461 aaaatgtgtt cttgatggga gaagacgtcg gtgtcttcgg aggagacttc ggtacttctg
     5521 ttggaatgct tgaagaattt ggtccagaac gtgtccgtga ctgtccgatt tctgaagctg
     5581 ccatctctgg agcagcagca ggagcagcca tgacaggact tcgtccaatc gtcgatatga
     5641 ccttcatgga cttctctgtt attgccatgg acaatatcgt caaccaagct gctaaaacac
     5701 gttacatgtt tggtggtaaa ggtcaggttc caatgactgt tcgttgtgca gctggtaacg
     5761 gagttggctc tgccgcccag cactcacaat ctctagagtc ttggtttact cacattccag
     5821 gacttaaggt tgtggcacct ggaacacctg cggacatgaa aggcctgctt aagtcttcta
     5881 tccgtgataa caacccagtt attatacttg agtacaagtc agaatttaac caaaaagggg
     5941 aagtgccagt tgatccagac tacacaattc cacttggggt tggtgaaatc aaacgccaag
     6001 ggacggatgt aacagttgtt acttatggaa aaatgcttcg ccgtgtggtt caagctgctg
     6061 aagaattagc agaagaagga atttcagttg aaattgttga cccacgtacc cttgttccgc
     6121 ttgataagga tatcatcata aactcagtga agaagactgg taaggttgtt ctggtcaacg
     6181 atgcccacaa aacaagtggc tatatcggtg aaatttcagc tattatttca gaatcagaag
     6241 catttgacta tctagatgca ccaatccgcc gttgtgcagg agaagatgtg ccgatgcctt
     6301 atgcacaaaa cctagaaaat gcaatgattc caacagttga aagtatcaaa gatgcaatcc
     6361 gaaaaactta taacaaagac tagagttaca taagataagt tatgtaaata aaacaaagct
     6421 gacgagaaac tttgtatctt ttaggtgcag agctcttcgt cattaatatc ttagattaga
     6481 gcacgggcta aaagctcgga aaaaagataa atctccttgg cttcatcgaa gccatcgtcg
     6541 atttcctatt tttcagtcgc ttttgacgcc ctttgtatca taaatagaat tggagaggtc
     6601 atggctgatg acaagctaag agcgactcct gcagctagaa agttagcgga tgatttaggg
     6661 ataaacctct acgacgtttc tggctcaggt gcaaacggtc gtgtccacaa agaagacgtg
     6721 gaaacttata aagacacaaa cgtggttcgc atttcgccac ttgcaaaacg aattgccctc
     6781 gaacataaca ttgcttggca ggaaatccaa ggaaccggtc atcgtggtaa aatcatgaag
     6841 aaggatgttt tggccctgct tcctgaaaat atcgaaaacg atagcatcaa atctcctgct
     6901 cagattgaaa aagtggaaga agtccctgat aacgtaacac catatggtga aatcgagcgt
     6961 attccaatga caccaatgcg taaggttatt gcccaacgca tggttgaatc ttacttgact
     7021 gcgccaacct tcaccctaaa ctatgaagtt gatatgactg aaatgttggc tcttcgtaag
     7081 aaggttcttg agccaatcat ggaagcaact gggaagaaga ctactgtaac agaccttctt
     7141 tcacttgcag ttgttaagac tcttatgaaa cacccataca tcaacgcttc attgacagaa
     7201 gatggcaaga ctattatcac tcacaactat gtcaatcttg ccatggcagt tgggatggat
     7261 aatggattga tgacacctgt tgtttacaat gctgagaaga tgagtctttc agaactggtt
     7321 gtagccttta aggatgttat tggtcgtacc ttggatggta aattggctcc aagtgagtta
     7381 caaaattcaa cattcacaat cagtaatttg ggaatgtttg gtgttcagtc ctttggtccg
     7441 attattaacc aacccaactc agctatcctt ggtgtcagtt cgacaatcga gaagccagtt
     7501 gtcgtcaatg gtgaaattgt gattcgccca atcatgagtt taggattaac cattgaccac
     7561 cgtgtcgtag atggtatggc tggtgctaag tttatgaagg acttgaaaga gttgattgaa
     7621 acgccaatct caatgttaat ttaagagcaa gagtattcat ttttaagata tagataaagg
     7681 aaggaagaca tggccttaga agtaattatg ccaaaagccg gcgtggatat gacagaagga
     7741 caaatcgtcc aatggaataa aaaagtcgga gaatttgtaa aagaaggaga aatccttttg
     7801 gaaatcatga ctgataaagt cagcatggaa ttggaagccg aagaagatgg gtacttgatt
     7861 gccattctca aaggagatgg tgaaactgtc cctgtaacgg aagttatcgg ttaccttggt
     7921 gaagaaaggg aaaacatccc aacagctgga gcagcatcgc cagaagctag ccctgtacct
     7981 gtagcaagta cctcaaacga tgatggtaag agcgatgatg cttttgatat cgttgtgatt
     8041 ggtggaggtc ctgctggtta tgttgcagcc attaaagctg cccaactcgg cggtaaggtt
     8101 gcccttgttg agaaatctga acttggtgga acctgcttaa accgtggatg tattccaacc
     8161 aagacctacc ttcataacgc tgaaattatt gaaaatatcg gtcatgctgc aaatcgtggt
     8221 atcgtgattg aaaatcctaa cttcactgtt gatatggaaa aacttttaga aactaaatct
     8281 aaagttgtta atactcttgt tggtggtgtt gcaggacttc ttcgtagcta cggagttact
     8341 gttcataaag gaattggtac aatcactaaa gacaagaacg tcttggtaaa tggttctgaa
     8401 ttgcttgaaa ccaagaaaat cattcttgct ggtggttcaa aagtcagcaa gatcaacgtc
     8461 cctggtatgg aatctccact tgtcatgact agtgatgaca ttcttgaaat gaacgaagtg
     8521 ccagaaagcc ttgttatcat cggtggtgga gttgtcggta tcgaactcgg tcaggccttc
     8581 atgacgtttg gttcaaaagt gactgttatc gaaatgatgg accgtatagt tccagctatg
     8641 gatgcggaag tttctaagaa ccttcgcttg atccttgagc gtaaaggaat gaccatcttg
     8701 actggtacta aactgcaaga aatcattgag gaaaaaggtc aacttcgtat caaggttgaa
     8761 ggaaaagacg atatcatagc aagcaaagct cttctttcaa ttggtcgtat gccagacctt
     8821 gaaggtattg gagaggttga gtttgaattg gatcgtggtt gtatcaaggt caatgaatac
     8881 atggaaactt cagttccagg catttatgca ccaggtgaca tcaacggtac taagatgttg
     8941 gctcacgcag ctttccgcat gggtgaagtt tccgctgaaa atgcccttaa aggaaatcat
     9001 gcagttgcca aattgaattt gactcctgca gccatctaca ctctccctga agtagcagca
     9061 gtaggtttga cagaagaaca agcccgtgag aaatacgatg ttgccatcgg taagtttaac
     9121 tttgctgcta atggtcgtgc tattgcatct gacgcagctc aaggtttcgt aaaagttatc
     9181 gctgataaga aatacggaga aatccttggt gtacacatta ttggtcctgc agccgcagaa
     9241 ttaataaacg aagcatcaag catcatcgaa atggaaatca ctgttgagga aatgctgaag
     9301 accatccacg ggcacccaac ttactctgaa gtgatgtacg aagcgtttgc agatgttcta
     9361 ggaatggcca tccattcacc taagaaaaaa taaaacgaag atagattaga ctggaaggtt
     9421 atttccagtt tctatcatat aaagtaaagg gatatcatga aatacattat caatcattca
     9481 aatgacactg cttttaatat tgccttggaa gaatacgcct ttaaacacct tttggatgag
     9541 gatcaaatct tcctactttg gattaacaag ccatctatca ttgttggtcg tcaccagaac
     9601 actatcgaag aaatcaaccg tgattatgtt cgcgaaaatg gtattgaggt agttcgccgt
     9661 attagtggtg gtggagctgt ttaccacgat ttaaataacc tcaactacac gatcatctcc
     9721 aaagaagatg aaaataaggc atttgacttc aagagcttct caactccggt tatcaatacc
     9781 ctggctcaac ttggggttaa agctgagttc acaggtcgta atgaccttga gattgatggt
     9841 aagaagttct gtggtaatgc ccaagcctat atcaacggcc gtatcatgca ccacggttgc
     9901 ttgctctttg acgttgattt gtcagtcctc gcaaatgccc ttaaggtttc aaaagataaa
     9961 tttgaatcaa aaggtgtgaa atccgtccgt gcccgtgtaa ccaatattat caatgaatta
    10021 ccaaaaaaaa tcacagtcga aaaattccgt gatttactct tggaatacat gaaaaaagag
    10081 tacccagaga tgactgaata cgtcttttca gaagaagaat tggccgaaat caatcacatc
    10141 aaggatacta agtttggaac ttgggactgg aactatggta aatcacctga atttaacgtc
    10201 cgtcgtggaa ccaaattcac tagtggtaag gttgaagtct ttgctaacgt tactgaatca
    10261 aaaatccaag acatcaagat ttatggtgac ttctttggta ttgaagacgt tgctgcagta
    10321 gaagatgtcc ttcgtggggt gaaatacgaa cgcgaagatg ttcttaaggc gctaaaaacc
    10381 attgatatca cacgctactt cgctggtatt agccgtgaag aaatcgctga agcggtagtt
    10441 ggataaatca aaaagaagtt ggccaactaa ccaacttctt tttgatattt atttacataa
    10501 tttaaaatac gtaaaattat aaactatcta gagcattctt ttgttcatca ttaacaatat
    10561 gggtatagag gtcagtgact tgtgtgctgg catgtcctag ttggtgactg actaaaactt
    10621 gtgatttagt cgcatcatag agcctagttg ctagcgtatg gcgcagttta tggggtgtta
    10681 cacgcacttt aaaatcctct gagtatttag caaccatttt ctcaacgcta gaagcatcga
    10741 tacgattagg aacacctctg tagagagtta aaaaaagggc tgtatctgtt ttttccgttt
    10801 tatagcgttg attccgaatg gccagataat tctctaaata aggtttagca aaagcagcga
    10861 cattgactga gtcacgtttg ccaccttttc gagtaacatc aataaccatc atttttagat
    10921 tgagatctct tagatctaga ttaacagctt cagataagcg aacaccagat gccaagagaa
    10981 gggcaataat ggctaaatct cgttctttat ttttgttgaa tgatgagaga gctcgatttg
    11041 aaagctgttg tgggtgctct tgatcgatat aagttagaaa accttctgtt tcatcaccta
    11101 gaaagagttt ttgcttgata ttttcagctc tggcagcaag ggtttctttc tttttcttgg
    11161 ttgaaacttt tttcattaca ttacgataga aataaggttc cccctgatcg ttttcaacct
    11221 cctcggttag atacttgtaa agactagaaa gtgctgataa ggttcgattg atagttgtct
    11281 gtgaaacacc ttgttttgtt gtattagcat tcagcaaggg acgttcacgt agataaagga
    11341 taaaggattc catgtctttc ttagacatat tttccaaagc tgataaagga atatcggata
    11401 ttttatcagc gtttgaaata ccagactcca aaacccagct gaaaaatcgg tcatattcct
    11461 taaggtattc gtacagggtt gtaaaactgt agggtacagc cagcttagat tggtagtatt
    11521 ccagaacata ccagggcatg agttgtttta gtttgtcgat tcgttccagt aaaatctcac
    11581 gtttcatgta ttttctccat aagtaagtat actagtagta tagcatagac ttgtatattt
    11641 ttcaagaaaa ttatagtttt tcagaaagat gttataggag tttttcaaag tgataaaaag
    11701 acttagacct acaaacaaaa cagaataagg aaaatctcac ccactaaact tctgttaata
    11761 ctacataact gaagagctat ataaggagct atgagataca aaacacattg tataatagat
    11821 tgaaactaga atagtacatc tggacttcta aaacattgct agaaattgat ttgattgtcc
    11881 tgattgtttt atcctatttc tatttcattt cactatataa ttgctattgt tttctttttc
    11941 atgattttaa tcttactgta cagatttgat acagtaaact cctttccagc tttatgcttt
    12001 atcatttcta acttaattat agtcttattt ttaataaagt tcaattattt atatgtaaaa
    12061 ttcctcatca gtaaaaaata accgatctca tttctgagaa tcggttttct aaataaatca
    12121 aactgatcat ttacataaca taaattatgt gaatgatcat actacaacaa caaatctttg
    12181 acttttccaa tttcactttt tggaataacc aggtgaatca tatcacccag atacattctg
    12241 gttgagccgt taactgtttg gctcttgcca ttatggactt gagttgtgat gaggacgttg
    12301 tgtggtaagt tgagttcatg aacttgtttc ccagcaattt tatcagaaac tggtatttcg
    12361 ataagtgtaa cttctccttc gctagatact tcttctggaa gcattttttc cagcatggct
    12421 tcatagactg gcgtaccttt gagcaaatcc atgataatat aagaaacaag agtgacaaga
    12481 ccaagtggca tgaggttgcg aatatctcct accatctcag ttacgaggat catagcggtt
    12541 aagggagctt ttgatatggc tccaaaatag ccactcattc ctagaatgac aaatataggg
    12601 aattgctctt gactgacaag tccaagattg acacagataa caccaactaa ggcaccaagc
    12661 aaagaaccaa gagctaaaat gggtaggaaa attcctcctg gcagtccact tccatagcta
    12721 atcatactcc aaataaagcg gatcaaaaag taagctaata aaacttggaa actaaaattt
    12781 tgttcagtta aagaaaggac aagctgattt ccgccaccaa ggatttgagg taagaagatt
    12841 ccgactggta tgataaggat aaaagccaag atgggataat aagccctatc caaatggatt
    12901 ttttgaccaa tcaagtcata aactcttcca acgtttaata cagctttctc atagagaaaa
    12961 cctgaaaatc caaggaaaat tcccatgacg agatatatcc aatactggtc tagggtcatg
    13021 ggaggaatgt tatctgacat atccaatact ggtgtcaaac cgaacatgag tagagacaca
    13081 aagtttgcta cgatgctggc tgctagagtt gagacccaga aaaagcgcga aaaatggtga
    13141 tagacttctt ctacaacaaa gagaagtgct gcaataggag cattaaaggc tgcggctaaa
    13201 cctgctgcag ctccactggc aatcaaggaa cgttcctcta ctggactgga tttgagccac
    13261 ttggcaattc ctttaccacc aactgctcca agttgaatgc tgggtccctc tcgacccagc
    13321 atgagtccac tggcaatagc aagaatacct agcacatatt ttttccaaag aatgccccac
    13381 cagttgaggg acatgaggcc tttcagttcg gcttcgactt gaggaattcc tgagccttta
    13441 atatcttttt ctgaccgtgt tagtttggca ctgagccaac agatgagtat ataaaacaaa
    13501 accagtacaa aaagattgcg cactaagtac ccttgatctt gataaactcc ttgtatcagg
    13561 tggaagccct tttcaattaa gaaacgaaag gatccgacga taattccaac gatgaggccg
    13621 acaatgattc ctcgaccaac ttgggatagt atagtgctgg atgcaaaggc gaattctttc
    13681 ttagaacgga ctatttccga ctgttcctcc atggtatttc ctttcaatta actttctttt
    13741 ttacctaaaa ccagtgattt aacgggttca aagctggttc ggtgaattgg ggtaactcct
    13801 agttttgtga ggccttccag atgtttagct gtgccatatc ctgcattagt agcgaaatca
    13861 tagccagaga actgctgatc gtattctttc atcaattcat cacgtgttac cttggctact
    13921 atagatgctg ctgcgataga gagggagttg gcatctcctt tgataatgga ggtttgtgaa
    13981 atgggcaagt ccagtttcat ggcatcaatc aaaaggtgct ctggttgagg gctgagctgg
    14041 gagattgctt cttgcatggc tagtttggtt gcttcataga tgttgacttg gtcgatgacc
    14101 tgattatcta tgataccaat tccaatcgac aaggcttggt cttgaacggc ttggaaaatc
    14161 tccagatgtt tctttttagg aattttcttg ctgtcgttga gacctttaat cttacaattt
    14221 ttaggtaaaa taacggccgc agcgactaca ggaccagcaa gaggaccacg accaacctca
    14281 tcaatacctg caattaaggt caatccttgc ttataaagtt ctttttcata agaaagcatg
    14341 gattccaagc gcaaattttc atctaattca gcttgaatgg ctcttttacg cttgctgatt
    14401 tccttttgaa ctcctgagcg attatccttt tcaagatcta aaaaaatagg gctttctaac
    14461 tccttgactg tcacaaggag ttctttgatt tctttaatcg ttgccatcga ggtcttccaa
    14521 tgtatctaag gtatagttac cgagtttgcc atcacggact tccttcacga agagactgta
    14581 aaaacggtca tagtcatcac ggaaaccgag ggcgcgggtc atatccataa taatcacagg
    14641 cgcttcttct tcaattttca tttgtttgaa gcgttcagcc agcttttctg gataatgttc
    14701 tttgaaataa ttgataccaa aaatggtaac ctcatccata ggaagcaact gatccttgat
    14761 agctccagtc aatgccaact taagtgcaac agtttcatcc tcaaacttag gccagagaat
    14821 ccccggtgta tccaagattt ccagatcttt attggtttta agccattgtt gaccttttgt
    14881 gacccctggc ttgtttccaa caacagcaat ctttttacca gccaaacggt tcatcagagt
    14941 ggatttacca gcgtttggaa tcccgataat catggtacgc aaggtttcaa tctgaatccc
    15001 acgttctttc tggcgagcaa tcttatcagc catgagcttc ttggccgcat ctgttacaac
    15061 ttttacagtc acttgctctt tggagttgat agctagcgtc tggattcctt gtgattcaaa
    15121 atactgacgc cattccttgg tcattgctgg atcagccaag tcggccttgt ttaaaatcaa
    15181 gagttttggt ttatcaccaa caatcttggt caacatagga ttttgactag atagaggcaa
    15241 gcgtgcatct actaaaatcg tcacaaaatc aacaaatttt aaattctcct gcacctgtcg
    15301 acgagcttta gacatgtgac caggaaacca ttgaatagta gccattgagt tttttccttt
    15361 catagcaagg atttagatcc cctattttat tttactatag tttgaacata gagagttttc
    15421 aaatgaatga tgtccaaaca gttcttgttt tatgacatag aaatctactt tatattatat
    15481 catgccttct ttattttgtc cgatgatagc cttttaccct atcttttacc ctatacccta
    15541 tcctataccc tatatagaaa aaagccatca aatgatgact tttcagaggg agcaaaaaac
    15601 tgtcctcttt atgttttaaa catttaaccc aaatcattca tacctctctc aactagatgt
    15661 aacttacaaa acccctgacc tcatgagcca ctttcttcct cctcatgagg tcagttttac
    15721 tttctgctgt tccagtatcg tttttcctcg ctagatttcc tcaaaagagc agactcctcc
    15781 cttggtgcgt cacacgattt tttcatctcg actgttcttt aatgcatcat taacgacgct
    15841 tttcttctag gtggttcata aggaacagga agattcaggt tgacttttct aatcctagaa
    15901 taaagtgctg aaaacaattc ggaataggca tagagactag acaatttgag gagctgcttg
    15961 cgtcctgttc gaacacattt tcctaccacg tgaagaaaaa gatggcggaa gcgtttgatt
    16021 gttaaagttt ggaagtcacc tccagataga tgtttgagaa aaagatagag attgtaggcg
    16081 atacagctca tcatcatacg aacttcgttt ttgattaag
//
LOCUS       DAVKGU010000039        15871 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKGU010000039 DAVKGU010000000
VERSION     DAVKGU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.039"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(1..297)
                     /locus_tag="VW077_001182"
     CDS             complement(1..297)
                     /locus_tag="VW077_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455205.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(281..2440)
                     /gene="pfbA"
                     /locus_tag="VW077_001183"
     CDS             complement(281..2440)
                     /gene="pfbA"
                     /locus_tag="VW077_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9455206.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYNIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(2720..2950)
                     /locus_tag="VW077_001184"
     CDS             complement(2720..2950)
                     /locus_tag="VW077_001184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455207.1"
                     /translation="MSDIQVNIPGECLYDKVFVLSFIIYNISTNLNIVNGIFYIQAKK
                     DSITFEWKAKEQTRKLAIDSSKPCFEVVDIVK"
     gene            complement(3570..4796)
                     /locus_tag="VW077_001185"
     CDS             complement(3570..4796)
                     /locus_tag="VW077_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9455208.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4839..5531)
                     /locus_tag="VW077_001186"
     CDS             complement(4839..5531)
                     /locus_tag="VW077_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9455209.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5704..7455)
                     /locus_tag="VW077_001187"
     CDS             complement(5704..7455)
                     /locus_tag="VW077_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455210.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(7445..9190)
                     /locus_tag="VW077_001188"
     CDS             complement(7445..9190)
                     /locus_tag="VW077_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455211.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9318..9848)
                     /locus_tag="VW077_001189"
     CDS             complement(9318..9848)
                     /locus_tag="VW077_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9455212.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            complement(9845..10301)
                     /locus_tag="VW077_001190"
                     /pseudo
     CDS             complement(9845..10301)
                     /locus_tag="VW077_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10435..11262
                     /gene="xth"
                     /locus_tag="VW077_001191"
     CDS             10435..11262
                     /gene="xth"
                     /locus_tag="VW077_001191"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9455213.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11274..11372
                     /locus_tag="VW077_001192"
     CDS             11274..11372
                     /locus_tag="VW077_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9455214.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            11417..11656
                     /locus_tag="VW077_001193"
     CDS             11417..11656
                     /locus_tag="VW077_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9455215.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12039..12134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12534..13115
                     /locus_tag="VW077_001194"
     CDS             12534..13115
                     /locus_tag="VW077_001194"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9455216.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13115..14377
                     /locus_tag="VW077_001195"
     CDS             13115..14377
                     /locus_tag="VW077_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9455217.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLTIVLNAVLNHKKK"
     gene            complement(14432..>15871)
                     /locus_tag="VW077_001196"
     CDS             complement(14432..>15871)
                     /locus_tag="VW077_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutH"
                     /protein_id="HEU9455218.1"
                     /translation="EYSHKLLNRKFSDVMEEYNRSLYKSYDDYNDRVVSEVQDKAISM
                     KSKGQYGNYIEKYFYGYQPNSDSEADFEKIGVELKVTPFKINKNGTLSAKERLVLTIL
                     NYMEENLEDFYSTHLWKKCAKILLLFYNGLIPNQTMKDYVIEKIFLYEWFEEDMAVIL
                     EDYQKITDKIKNGKAHELSESDGNYLSTCTKGAGKGKDLRQQPFSHELAKQRAWELKS
                     SYMTYLINHKIFNQSDQESVLANFRGEKKSFTEVIAEKILSYKGFSEQELYDRFEVNS
                     KAKGKNSTLIRKILGLTGDLDKTKEFQKANMNLRVIRVDKNNLPKEDSPFKTYCFKEL
                     AATDSWESSHVYNEIYNKRFLFVIFKEIEPKLFVLDSIKFWGFQDRQLEEIQRVWQET
                     RQIISDGVKLTQNGNKVSTNFPQSKINKILFTKLHATNTYYEIDKGKFVGKGSLSDTD
                     ELPDGRRITKHSFWMPKKFIKEILDGNWD"
BASE COUNT         4700 a         3342 c         2883 g         4946 t
ORIGIN      
        1 ttatttcatt ttactatact ctgttaattc atatgagttt aaaccgattt catctttaac
       61 ctcgagtaaa gcagtttcaa atatttgttt aagagttttt gattctttac aattaaccga
      121 caaactttct gataaaatat gtacaacttc tgagactgaa taacctatct cctctttaga
      181 attatataaa tctgtagctc caccaataat ccaaaaatac tgattttgtg aacctacaat
      241 atcctcattt tctacggaac ttccttgtat cgaacaaatt ttatttatct ttaccataat
      301 acttcaaccc ttttagtgtc aaaagtaaac caattcctgt cactgttaag aatagttcca
      361 taatcttgtt cgaaccagtc tttggtaatt tttgttttac atctactatt tctttagatt
      421 tattaatatg attttcagtt tctctgccat ctccaactat tttatagttt acttcttctg
      481 tcttattatc ttgtttattg tcgatcttgt cattcatttg tctattatct ttacttgagt
      541 taaactctcc gttcttctgg ttactatcaa ttacattatt tgaattagat tgtttttcct
      601 ctttgttttt ttctttttcg tttttatcac ttaaattatt tgttacaata ttgtaaagcc
      661 cattctccgt tacaatattg aaattaccat cgctatcacg tataacaggt tctttcccat
      721 ttgcattaga tttgatgaat gatatatact taccggataa attataaaat tggttattta
      781 aaacggttat tttacccttt gaatcctcaa taacaattcc ttctttaccc ccgaatatac
      841 tattctctga gactactaaa ttatcactaa ctcgtaataa ttcaatattt ggttgttctg
      901 tttccttaga attattatta attacatttt ttgttacagt aatatctgat acttttccta
      961 aatattctgc actatctttt gcaactcgta tcgcttttgt tttaggatcg gcaatattaa
     1021 atatattttc ggcgataacc acctgtttat ttaaatctaa taggtcttta gtgtttttat
     1081 agctataagc atttactaaa gctgctccgc tttctcgata atgtacactc tctcctttaa
     1141 ctttcttatc aaagcgattt cctttgatta atacatcagt gaatcctgta aaacgtacac
     1201 ctgcatacat catgttatca aaatgattat ttaaaatttt aatattagag gggttctgtg
     1261 tcgacaatgt ttgatagtgt gtgccaattg ctgttactaa ttccccagat ttatcacttt
     1321 tgccaaaata ggaattttga atagtcacat tttcagattt tttcccatca tcattcaagg
     1381 cataaggaaa accttttcta gttaatggtt caatctgaat gctctcctta cttatgattt
     1441 gcccatcctt catcgttttg ggtaaggctt gcccaagaaa acgagaatta tcaactaata
     1501 catttttcga acctgcaatt tgaatagcat gcccttgata actatccttg aatgttacat
     1561 tttttatagt tacgttattt gaattcccaa tagcaaatgc acctgaagaa tttataagtg
     1621 gtagattttt tgctttagtt ccttcttcat tcaaagcacc gttcatatca atcgtaccac
     1681 cagaataact aatatcttct gttgggcccc attctacttg cgcaccatca tccgtaaata
     1741 aacctgtcat aaaaacaatg gaagggtgat tcttaatatt tataccattt agaattgtag
     1801 ctttctcatt caattctaag tgtgtatgac tttttaaaaa aacaatttct tttactaaat
     1861 aagttccttc aggaaaatat acatttcctc cacctagccc ttgagctgca gcatctattg
     1921 catcttgaat tgcttgtcga tcatcatgaa tcccatcacc taccgcacca taatctttta
     1981 cacttaaagc agtatcttca aattcttttt tgataattct gtcacttata acatcttcct
     2041 tataactctt attttcatta gtagatgtta ctgtagattc aactgttctg ttgtctaatt
     2101 gtgaattgga acgaatagta ccattagagc tgatactatt aacacttaac gaattatcta
     2161 aaccattagt ggttgaatta gaggcaatca tttcagcaga acgattctga ttaacagttg
     2221 gtgaattatc taaatcatta gtaattgcat tagaagactc ttttgtagcc atcggtgaag
     2281 aactagtaac aacttcatcc gataaaatac cttggcttcc caaaattgaa attgccaata
     2341 gtaccgagca agtccccacc tttaatttac gaatactgaa tacctcatta ggaacaaaat
     2401 atttcatcag aacctccaat ttttttacta tttttaacaa ttgattatta cttttcttaa
     2461 aatagataga atgacaccgc tgtcattatc tttatcatat attttccaaa ttgaattgtc
     2521 aaggaataac acaagaaaat attaaaattt atactcttcg gaaatctctt caaactaggt
     2581 cagtgccgcc ttgccctaag tctaggttac tgacttggtc agttctatag tagactgaaa
     2641 ctaaaatagt acacctctac ttctaaaaca ttgttagaaa tcgatttgac tgtcccgatc
     2701 aatttgtcct attcttattt cattttacta tatctacaac ctcaaagcag ggttttgagc
     2761 tatctatggc caacttccta gtttgctctt tagccttcca ttcaaaagtg atgctatctt
     2821 tcttagcttg aatataaaat atcccattta ctatatttaa atttgttgat atgttgtaaa
     2881 ttataaatga aagaacaaat accttgtcat acaagcattc gccagggata tttacctgaa
     2941 tatcgctcat atggcggatt gaaactaaaa cactacaata tatttcttaa atagaccttc
     3001 tataaaagct agatttaacc tcgataaaaa tagatgcagt gcttctaaaa tactggaata
     3061 gggcatatag gtaatcgtag acaagtgttt tatagattgt ctcgcaggag atctaatgat
     3121 gtatagcaag catcactaaa catatagaat atttgtatta ttataatatc aacatgctat
     3181 aaattctatc atgcaaaaga atgactagaa catgaaagac aggcacaaaa ttttgaacaa
     3241 tattctgaaa acaaaaaaag acgcgagttt ccacaatctc actcatggaa actcacgtct
     3301 ttcaaaaaat agggtgaatt gaaaactaga acaagacact agattttcta aatgatgtct
     3361 agtaaggatt ctaaatgcta cactaaattg ctcacatctt atttcaaacc acgggactcc
     3421 cacaagaaag gaaaatccta taaaaattct ctctctgagg agaaagaggc ctcaactaca
     3481 ggtgtaaact attatcaatt gaaaacaaca gtttttacac tcaattagac tttttaggga
     3541 caggatcact ttcgcttgac tgtagtttgt catttttttg ataaagttag cactttttca
     3601 gaaactgttt tgaaagtctc aatgatatag tctacttctt catcgcttaa tttagtatga
     3661 agagggaggg taatttcatt ctcaaagaag gcataggcct taggatagtt cgtcatatca
     3721 aatccaagat tcttataggc tgtcaagaga ggaagcggtt tgtagtgaac attacttgca
     3781 attcctgctt tagccaattc ttggatgatg aggttgcgtt cttctaggct tgctccttct
     3841 acacgggtga tgtagaggtg gcgtgaagat tcgacagttt cagtcttgtg tgccaaagga
     3901 tggatgcgag aacctgcaaa accactatca tagcggtcca caatgtcctt acggcgttgc
     3961 aacaaacttg gatagcggtc caattgtacc aaaccaagtg aagccatgat atcggtcatg
     4021 ttgcacttat aggctggtgt aacgatatcg tattcccatg accccagttg catcttggca
     4081 agagcatcct tagtttgccc gtgaagggaa aggatttgga attccttgta catctcttcg
     4141 tcatcaatca ctggattggc tttccaagtc gcacttccac cttctgccgt tgtaaagttc
     4201 ttaacagcat ggaatgagaa ggaagtaaaa tcagcgatag aaccagaagg ttgtccttta
     4261 taagtagatc ccaaagcgtg ggcactatca gagacaatga caatacggtt aaaggccttt
     4321 tgccacttgc ttgaagcggt aaagaagtca cgttttttct ccacgacttg gaacaaacgg
     4381 tcataatcgc aaacaatccc tgcgagctct actgggataa tcaccttagt tttctcagtg
     4441 atagcttgct caagcaggtc atagtccatc tcaaacgtat ctgcttggat atccaccatg
     4501 acaggggttg ctcccacgtg cgtaatgaca ctacatgaag ccgtataggt catggctgga
     4561 acgatgactt catcaccagg tcccacttcc aaaacgcgta aaatcaactc cagagcggct
     4621 gtcgcagagt tgagacaaac agtcttaggt gtctgtgtgt aaagagacaa gcggcgctcc
     4681 agttcttttg ttttaggacc tgttgtgatc caaccagaac gcagggtatc cgctacttca
     4741 gcaatttctg cttctgtgat atcaggcggt gaaaatggaa tattgtaatt tggcattgat
     4801 ttgctccttt tatctgtact cattttctta tgactacttt attttagtac ctcaaacacg
     4861 gtttgaaaca tgattttgat gtccccaaag aaactaaact ctcggagata ggcgaggtta
     4921 tagcgcatct tttcaggaag aacatgctcc acataggcct gatcaactga cagacctttc
     4981 tccgtcattt gactgatgat ggtgtcctca tccttgtagt tgatgctggc tggagaggta
     5041 attcctgctt gcaagagcaa ggttgccatc atttcagggc tatactgctc tgtataacgt
     5101 ggcacttcag gtcgtgtacc gacaaaggac atctcacctt taaggacatt gaccaactga
     5161 ggcagttcgt ccaaacggac acgtcggatg aaatttccaa ccttggtaat acggctatcg
     5221 ttagcagaag tcaccagact tcctttttta tccgcatccg tcaccatggt acggaacttc
     5281 caaatcttga accgacggtt gtactgggtc acacgctctt gcttgtaaat cactggcccc
     5341 ttgctatcca acttgatcca aatgctcaag atgagaaaga tgggagaagt tagaaccagt
     5401 aagaccaagg ccaaaaccca gtccaagcaa cgcttgaaaa tcagcgaacc cttcctttta
     5461 gagacaagct ggtagtaaga ctcaacctcg cttgatttca tttccacagg caagtcttcc
     5521 catttcagca tgctgttctc ctttgttttt attatactat ttgtcaacat ttttcattat
     5581 accacaaaat ggaaaaggcg gtgaaagaaa tctgtaattt gaaggatttc cttctagtac
     5641 tcaatgaaaa tcaaagtaca gctttgagat tgcagataaa gctgacatgg tttaaagaga
     5701 ttttcaaaga gtataggcca tggcccctgc ctgcaaacta tacatcttgt gataggttcc
     5761 tcccagagtc aagagttcct catgggttcc actctcgata atgcgtccct tatccaagac
     5821 atagatgcag ttggcgtctt gaatagtaga aaggcggtga gcgatagcaa tagttgttcg
     5881 gccctgtctc atcttcgcca gagaagcttg aaccaaactt tctgtttcag agtcaatatt
     5941 ggctgtcgct tcatccaaaa tcaggatttt aggctggctg gcgactgttc tagcaaaggc
     6001 aagaagctgg cgctgcccag tagagaagct cgaaccacgc tcggaaacag gggagtcgta
     6061 cccctgagga agttcttgaa taaaggaatc tgcatccaca aaggctgccg cagcctgaac
     6121 ctgctcatca ctgatttctt ggtacatggc gatattggac ttaatagttc catgatagag
     6181 gaagggttcc tgtaaaacca aaccgatgtt ttttctcagc tcttcttgac tgaaatccct
     6241 gatatccaca tcatccaaga gaactctccc tgactggaat tcataaaagc gcatgaggac
     6301 attgataata gacgatttcc ctgaacctgt atgacctaca aaggcaatgg tttcaccctt
     6361 attaacagag aaagaaatgt catccagaat cggatgttta ccgtcatacg agaaacacac
     6421 atgttcaaaa cggatattgc cttctttgac tttggcttgc ccattttctt gaaggggctc
     6481 ataggtcctc tcatcgatta aggcaaagac acggcctgca gaaaccatag acgtttgcag
     6541 tgttgaaaag ttttgcgtca cctcaatcaa agggtcaaag aggcggttga tgtactggat
     6601 aaaagcatac atggtcccgg ccgttatccc gatagaaaga ccacgatagc caaaataggt
     6661 catcaagaca gcataaccta gaagtttcaa caaactcatg gcaggtctca aaaagagggc
     6721 atccaaggct acagaacggt tggcataggc caagtgctct tggttgattt catcaaattc
     6781 tgcctgcagg cgcttctctt gattaaaagc ctgaataatc ctgattccct cgatattctc
     6841 tgccagctta ctattgatat ctgacaagag acttctggtt ttctcgatga ttttcactga
     6901 cttttttcga tagagattga ccaaaaggaa aatcaagggg agaaagagca agactaaagc
     6961 cgtcaaacga aaatccagca ccaacatggt ataaagggtt gtcagaaaga taaaaactgc
     7021 tgagataaag ctggataaaa tcccagaaaa catatcacta atcgtctcgg tatcgttggt
     7081 caaacgagaa acgatagaac ctgctggcgt cttgtcaaag taagacatgc ccagtttctc
     7141 catattggca aaggcatccc gacgaatatc cctaacaata ctgtaagaca cgcgcgcaaa
     7201 gagaagattg ccgacatact gaactacagt ttgtaggatg tagagaccat agtagaccag
     7261 caaaacggtc acggctagtt ggttaagatt gctgagatac tggtcgataa agtgggaagc
     7321 cacaagggga atgacacttt taatgaccgt cgtcgctagg agaaaactga gtgccaaaaa
     7381 ggtaaggagt ccataaggct tgagatagga catcaagcgc ttcaatacag tccattgttc
     7441 ttgtttattc tgcatcttct tctcccttca tttccagctg ctgagactgg taggtttggg
     7501 cataccagcc atccaaagct agcaagtctt cgtgcctgcc tcgttcgata atttgaccat
     7561 tttgcaggac taaaatcaaa tctgcatgga caactgcact caggcgatgg gcagtgataa
     7621 tggttgtctt gtcctttcgc gtctccttga ggttgtcgat aatcgcatac tctgtcttgg
     7681 catctacggc ggataaggaa tcatccaaaa tcaagatatc agggtctaaa atcatagccc
     7741 gactcatagc caaccgttgc ttttgaccac cagaaagact gactcctttt tcaccaatca
     7801 gcgtatcaaa tccttgaggc atgtctacaa tatcttggta aactcgggct agcttagtag
     7861 cttcctcgac cgctgaaagg ggcaagttag gattgccaaa gcggatattg tctaggattg
     7921 aagtcgcaaa aagaaactgg tcctgaggaa catagcccat gagactgcga aggtctgtca
     7981 gacgatagtc ccgaatatcg tgaccgttta gataaatggc gcccttatcc acatcgtatt
     8041 cacgcaagag gagcttgatt aaggacgttt tcccagagcc tgtctgccca accaagccca
     8101 gtgtttgccc ttttgccaaa ctaaagtgaa tatccgtcag tgtttcctca ttttcaaaag
     8161 caaagctgtc aatggcatac tccaaacgcc cattttcaat accatccaga ggaaactccg
     8221 ggtcttgtac aggagattcc tgagacaaaa gattttcaat ccgctggtaa gaaaccttcc
     8281 ctcgctgagt agtattaaag aggaagccga tggccataag aggccagacc agcatatcca
     8341 aatagctgat aaaggtgact agattcccaa ctgtaatctg cccttcctga accaccaagg
     8401 agccaaccaa aagcgttaaa acataggacg aaccaacaaa caagagaacc atagggtcaa
     8461 agagactatc atatttcatg gtttgcaggt tcttttggaa ggttaattca ttgactgcct
     8521 gaaaagactt caactcgtct gcctgataac cgaaagactt ggtcactttg atacctgata
     8581 cggactcctg taccttgtta ttgagttcag aaaaagcagc ttgggattcg ccaaaggcct
     8641 tatgagtctt tctccctagg cgactagtcg cataggccat gaaaggtagg gggagaatgg
     8701 caacaagagt catctgccat gagatgctaa agagcatggt caacaaagtc accagagccg
     8761 tgatagaggc atccaccgca gacatgacac cgccacctgc taaacgagtc aaggcattga
     8821 tatcattggt tgcgtgtgcc atcagatcac ccgtccgata ggtttgataa aaggctgacg
     8881 acatttttgt gaaatgctta aacaagcgag accgcatgat ctgtcccaag caataagagg
     8941 tcccaaggat atacatacgc cacacatagc gcaaatagta cataccaaag gctgcaagta
     9001 gcaagtaaaa taggctaaga aggaggtcct gctgggttaa ttgccccgat gtgatggcat
     9061 caataacccg ccccataacc ataggaggaa tgagattgag gacggaaacc aagaccaggg
     9121 ccacaattcc gactagataa cggcgttttt ctaacttgaa aaaccaccaa agtttttgaa
     9181 taatggacat aaaatccctt tctgattgca aatggaaacc tgaggccaat actcaatgaa
     9241 aatcaaagag caaactagga aactagccgc aggctgctca aagcactgct ttgaggttgt
     9301 agatagaact gacgaagtca gtaacctaca tacggcaagg cgacgttgac gcggtttgaa
     9361 gagattttcg aagagtacaa gaccccaggt ttttcttttt cataagttac gactgtaaca
     9421 gcacccttgt catattcagc aataaagata ttggctacat tgtcatgccc ttgtttactg
     9481 aggttatcaa gcaaccactc ttcgctacga ccaatcgatt ccaagacatc tacttgaatc
     9541 acaccgtcag tcacaactgg atacttagga ttttcatctc ccatttgcac aacgataagt
     9601 tgcccatttt gctcttgcac agctcgtttg acttgcttca tctggaaaat cccttggcta
     9661 cgaagtttga gggctacatc cgatgcagac aaaccaactg aacgacaggc ttctgggtca
     9721 atattcccat ttttgataag gagagttggc ttaccatcaa tcaaacgttt cacaaagtga
     9781 acattgttgt tgagccattt gagagtcaag accagtttga ttaaaatttc gatataatta
     9841 agtgtcattt gttcacatcc accagttcaa ttgcgtctgt cttatacaat tcgactttct
     9901 ctaacaggta cttgtctggc tcacttccgt ttagggcacg ataatagcga tctcccacct
     9961 tgataagtgc gccatctgtg tttgtggaag tattaacata gacttctgac ttgtctactc
    10021 ccaaatcttt ggaaacaacc tcgatgaaat gaagtgaagt ttgaaattta ttgttagaaa
    10081 cttgattgtt ttgataagtc gtgatgctga ccaaaagcag agctaaataa ggccagagct
    10141 gaaatcatga ccaactcacg aaattcggtt ccctttttat catggtatgc cttgaaagca
    10201 aaaaaaactg tcacagctaa tagaacaatc ccaaagccaa ccatgatacc attttgctga
    10261 ccgatttggc tgagtacata gtcataagag tagaatttca tatattttca ctccctttca
    10321 taaaatcatt cttaattata aacgaaagag agtaattttt caaaccatac gttggggaaa
    10381 tagatctttt tttggtataa taaaatctat aatctgaatg aaaaaggtaa ctttatgaaa
    10441 cttatctcat ggaatattga ttccctaaac gctgccctaa ctagtgactc agctcgtgcc
    10501 aaattgtccc aagaagtcct acaaaccttg gtcgctgaaa atgctgatat cattgctatc
    10561 caagaaacca agctttctgc caaaggacct acaaagaaac acgtggaaat tttagaagaa
    10621 ctcttcccag gctacgaaaa cacatggcgc tcttcccaag agcctgcccg taaaggctat
    10681 gctggaacca tgttccttta taagaaagaa cttacgccta cgatcagttt tccagaaatc
    10741 ggtgcccctt ctaccatgga cttggaaggc cgtatcatca ctctagaatt tgatgcattt
    10801 ttcgtaaccc aagtttacac tccaaacgct ggtgacggtc tcaaacgctt ggaagaacgc
    10861 caagtctggg atgttaaata tgctgagtat ttggctgaac tagacaaaga aaaaccagtc
    10921 cttgcgaccg gtgactacaa cgtagcccac aatgaaatcg accttgcaaa tcctgctagc
    10981 aaccgccgtt cacctggatt tactgacgag gaacgtgctg gatttaccaa ccttttggca
    11041 actggattta cagacacctt ccgccatgtt catggcgatg ttcctgaacg ctacacttgg
    11101 tgggcacaac gcagcaaaac ttctaaaatc aacaatacag gctggagaat cgactactgg
    11161 ctcacaagta accgtatcgc tgacaaggtc actaaatctg atatgattga ctcaggtgct
    11221 cgccaagacc atacaccgat tgtcttggaa attgacttgt aaggattttc tatatggact
    11281 acaatgcggt cattcccgag tttcttgtat cgaatatcga acagtcccgg tccttctact
    11341 gtggtttact gggttttagg attgaatact aacgtccaga agaaaacttt ctctttctct
    11401 tgaagagtgt caactaatgt tagaggaggg aacaaaggat caactagctg aactgaccta
    11461 cccctttggt cgtggagtca atctctcctt tggcataaag gatgttccaa aactctatca
    11521 aaaagtgatg gaggcaaact atcctattta tcgtctcttg accaaaagaa agtttcgtgt
    11581 cagtgatccc tatatctatc ctcataaatt tgcagttttg gatccagatg gctatttttt
    11641 aagatttagc gagtagaata ataaaaattg ggaaaagtct ttaaagttag aaaaaggcat
    11701 attgtcaggt gtctaaaaac ttgatagtat gccttttatg atggaaaaat cttctaatac
    11761 tcttcgaaaa tctcttcaag ccacgtcaac gtcgccttac cgtaggtata tgatactgac
    11821 tctgtcaatt ctatccacaa cctcaaaaca gtgttttgag gttgtggata gcttcctagt
    11881 ttgctctttg attttcattt agtataaatg ttagcttaca atggagtttt tgttcaccct
    11941 ctcttatttt tgttcgcttt ttaaaccaaa acactaacaa aatagttgat ttctaaagtt
    12001 atttagtgta aaataaaaac attggctcaa gcctatttaa aattgaatat cgttttactt
    12061 gtttatgtcg tgaattggca cgacgtttct acaaggtgcc ggaacaccta acaataagta
    12121 agtcagcagt gagatatggc cgtttttgcc atgtctattt aggggactta cttagagatt
    12181 aagtaggtct tttttgaata tttttaatat ttcccttaat tagtgcggac gggaggtgaa
    12241 attatcctgt gaataatttc aggaatccag ggaatttcat ccctaatttt tgagccccta
    12301 gctctttcat ttctaggctt aggctaaaat agttccccga actattttac tctcaaaaat
    12361 cccgtcatag acaataacaa gattattgtc tatgatacca ctacaacggg aaatatttga
    12421 taagactcgc ttcgctcgta atagttttat caaatatata gtattagcaa aagggatcac
    12481 tatagaaaca tttaaacaac aagtaaatta acttttagac tttaggaggt cttatgaaat
    12541 tattagaaga gcgcatcctc aaggatgggc atatcttggg tgataacatc ctcaaggtag
    12601 attccttttt aacccaccaa gttgacttta gcttgatgcg agagattggt aaggtttttg
    12661 cggaaaaatt tgctgctact ggcattacca aggtcgtaac cattgaagcg tcgggtattg
    12721 ccccagccgt ttttacagct gaagccttaa acgttcccat gattttcgcc aaaaaagcta
    12781 agaacatcac catgaacgaa ggcatcttaa ctgctcaagt ctactccttt accaagcagg
    12841 tgaccagcac tgtttctatc gctggaaaat tcctctcacc agaggacaag gttttgatta
    12901 tcgacgattt ccttgctaat ggccaagctg ctaaaggctt gattcaaatc atcgaacagg
    12961 ccggtgccac agtccaagct atcggtatcg tgattgagaa atccttccaa gatggtcgtg
    13021 atttgcttga aaaagcaggc taccctgtcc tatcacttgc tcgcttggat cgttttgaaa
    13081 atggtcaggt cgtatttaag gaggcagatc tctaatgcaa actcaagaaa aacactcgca
    13141 agcagccgtt ctgggcttgc agcacttact agccatgtac tcaggatcta tcctggttcc
    13201 catcatgatt gcgacagccc ttggctattc agctgagcag ttgacctacc tgatttctac
    13261 agatatcttc atgtgtgggg tggcaacctt cctccaactc caactcaaca aatactttgg
    13321 gattggactc ccagtcgttc ttggagttgc attccagtcg gtcgctccct tgattatgat
    13381 tgggcaaagc catggtagtg gcgctatgtt tggtgccctt atcgcatctg ggatttacgt
    13441 ggttcttgtt tcaggcattt tctcaaaagt agccaatctc ttcccatcta tcgtaacagg
    13501 atctgttatt accacgattg gtttaacctt gatccctgtc gctattggaa atatgggaaa
    13561 taacgttcca gagccaactg gtcaaagtct cttgcttgca gctattactg ttctgattat
    13621 cctcttgatc aacatcttta ccaaaggatt tatcaagtct atctctattt tgattggtct
    13681 ggttgttgga actgccattg ctgctactat gggcttggtg gacttctctc ctgttgcggt
    13741 agctccactt gtccatgtcc caattccact ctactttggg atgccaacct ttgaaatctc
    13801 atctattgtc atgatgtgta tcatcgcaac ggtgtctatg gttgagtcaa ctggtgttta
    13861 tctagccttg tctgatatca caaaagatcc aatcgacagc acgcgccttc gcaacggtta
    13921 ccgcgcagaa ggtttggccg tacttctcgg aggaatcttt aacaccttcc cttacaccgg
    13981 attttcacaa aacgttggtt tggttaaatt gtcaggcatc aaaaaacgcc tgccaatcta
    14041 ctacgcagct ggtttcctgg ttctccttgg actgcttcct aagtttggtg cccttgccca
    14101 aatcattcca agctccgtcc tcggcggtgc catgctggtg atgtttggtt ttgtatctat
    14161 tcaagggatg caaatcctcg cccgagttga ctttgctaac aatgaacaca acttccttat
    14221 cgcagctgtt tcaatcgctg caggtgtcgg tctcaacaac agtaatctct ttgtcagcat
    14281 gccgacagcc ttccaaatgt tcttctcaaa cggaatcgtc gtagccagcc tactcactat
    14341 tgtcctcaat gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt
    14401 tttgtttatc acaaattgat tataaataca atcaatccca attcccatct aatatttctt
    14461 taataaattt cttaggcatc caaaaggagt gttttgtaat cctacgtcca tctggaagtt
    14521 catccgtatc agataatgaa cctttcccaa caaattttcc tttgtcaatt tcataataag
    14581 tattggtagc atgtagtttc gtaaatagaa ttttgtttat cttactttga gggaaattag
    14641 ttgaaacttt attaccgttt tgagttaatt taactccgtc agaaataatt tgtctagttt
    14701 cctgccatac tctttggatt tcttctaatt gtctatcctg aaatccccaa aacttgatag
    14761 aatctaatac aaatagctta ggttcaattt ccttaaaaat aacgaaaagg aatcgtttat
    14821 tataaatttc attataaaca tgtgatgatt cccaactatc tgttgctgct agttctttaa
    14881 aacaataagt tttaaatgga gagtcttcct taggtaaatt attcttatct actcgaataa
    14941 ctctcaaatt catgtttgct ttttgaaatt cttttgtctt atctaaatcc cctgttaagc
    15001 ctaaaatttt tctaatcaga gtactattct taccttttgc tttggaattc acttcaaatc
    15061 tatcatataa ctcttgttca gaaaaacctt tgtaggaaag aatcttctct gcaataacct
    15121 cagtaaatga ttttttctca cccctaaaat tagccagaac actttcttga tctgattgat
    15181 taaagatttt atgatttatc agataggtca tataacttga ttttaattcc caagctcttt
    15241 gttttgctaa ttcatgacta aaaggttgtt gtcttaaatc ctttccttta cctgcccctt
    15301 ttgtacaagt tgataaatag tttccatctg attctgataa ttcatgtgcc tttccatttt
    15361 tgattttatc tgtaatcttc tgatagtctt caaggatgac tgccatatct tcctcaaacc
    15421 attcatataa aaatattttt tcaatgacat agtccttcat tgtttgatta ggaattaagc
    15481 cattgtaaaa caataacaaa atctttgcac attttttcca taaatgagtg gagtaaaaat
    15541 cctctaaatt ttcctccata tagtttagta ttgttaatac aagtcgttct tttgcactaa
    15601 gtgtcccatt tttgtttatc ttaaaaggag taacttttaa ttctacacct attttttcaa
    15661 agtcagcttc cgagtcacta tttggctgat aaccatagaa atatttttca atataatttc
    15721 catactgccc ttttgacttc atactaattg ctttatcttg tacctcagag actactctat
    15781 cattataatc gtcatatgat ttataaagcg aacgattata ctcctccata acatcggaaa
    15841 attttctatt taatagttta tgagaatatt c
//
LOCUS       DAVKGU010000040        15677 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKGU010000040 DAVKGU010000000
VERSION     DAVKGU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15677
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.040"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            72..506
                     /locus_tag="VW077_001197"
     CDS             72..506
                     /locus_tag="VW077_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9455219.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            518..1708
                     /locus_tag="VW077_001198"
     CDS             518..1708
                     /locus_tag="VW077_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU9455220.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANNQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1719..2210
                     /locus_tag="VW077_001199"
     CDS             1719..2210
                     /locus_tag="VW077_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9455221.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELNQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2225..3004
                     /locus_tag="VW077_001200"
     CDS             2225..3004
                     /locus_tag="VW077_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9455222.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            2991..3809
                     /locus_tag="VW077_001201"
     CDS             2991..3809
                     /locus_tag="VW077_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9455223.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3809..4102
                     /locus_tag="VW077_001202"
     CDS             3809..4102
                     /locus_tag="VW077_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9455224.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4124..6025
                     /locus_tag="VW077_001203"
     CDS             4124..6025
                     /locus_tag="VW077_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9455225.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLIDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKGYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6085..7086
                     /locus_tag="VW077_001204"
     CDS             6085..7086
                     /locus_tag="VW077_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9455226.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7196..7531)
                     /locus_tag="VW077_001205"
     CDS             complement(7196..7531)
                     /locus_tag="VW077_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455227.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7542..7784)
                     /locus_tag="VW077_001206"
     CDS             complement(7542..7784)
                     /locus_tag="VW077_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455228.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7800..7994)
                     /locus_tag="VW077_001207"
     CDS             complement(7800..7994)
                     /locus_tag="VW077_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9455229.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWKEDF"
     gene            8160..9110
                     /gene="rsmH"
                     /locus_tag="VW077_001208"
     CDS             8160..9110
                     /gene="rsmH"
                     /locus_tag="VW077_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9455230.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLRETGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            9122..9439
                     /gene="ftsL"
                     /locus_tag="VW077_001209"
     CDS             9122..9439
                     /gene="ftsL"
                     /locus_tag="VW077_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9455231.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            9443..11695
                     /locus_tag="VW077_001210"
     CDS             9443..11695
                     /locus_tag="VW077_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU9455232.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGSKGNGITY
                     ANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRVGNIVPGTELVSQQTVDGKDVYTTLSSPLQSFMETQ
                     MDAFLEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITEDFVWRDILYQSNYE
                     PGSAMKVMTLASSIDNNTFPSGEYFNSSEFKIADATTRDWDVNDGLTTGGMMTFLQGF
                     AHSSNVGMSLLEQKMGDATWLDYLKRFKFGVPTRFGLTDEYAGQLPADNIVSIAQSSF
                     GQGISVTQTQMLRAFTAIANDGVMLEPKFISAIYDTNNQSVRKSQKEIVGNPVSKEAA
                     STTRNHMILVGTDPLYGTMYNHYTGKPIITVPGQNVAVKSGTAQIADEKNGGYLVGST
                     NYIFSVVTMNPAENPDFILYVTVQQPEHYSGIQLGEFATPILERASAMKESLNLQSPA
                     KNLDKVTTESSYAMPSIKDISPGELAEALRRNIVQPIVVGTGTKIKETSVEEGTNLAP
                     NQQVLLLSDKVEEIPDMYGWKKETAETFAKWLDIELEFEGSGSVVQKQDVRTNTAIKN
                     IKKIKLTLGD"
     gene            11697..12677
                     /locus_tag="VW077_001211"
     CDS             11697..12677
                     /locus_tag="VW077_001211"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9455233.1"
                     /translation="MFISISAGVVTFLITLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLIAAVVVSFLLALFSKQLTNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFSYQVHLGIFYIIFALFWLVGF
                     SNAVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLSFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13241..15346
                     /locus_tag="VW077_001212"
     CDS             13241..15346
                     /locus_tag="VW077_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9455234.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGIGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVVIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLT
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
BASE COUNT         4823 a         2764 c         3530 g         4560 t
ORIGIN      
        1 aagataaaaa taaaggatta caatggggaa tataaagtaa accggtaaac ctaaacaaga
       61 aaggagaaaa gatgaaaatt gtacttgtag ggcatggaca ttttgctaca gggatttata
      121 gttctttaca attgattgca ggcaatcaag aaaatgtgga ggcaattgac tttgtggaag
      181 gaatgtcagc agatgaactc aagcaaaaaa tcttacttgc aatttcaaat gaagaagaag
      241 ttttaatcct aagtgatctc ttgggaggat cgccattcaa ggtttcttct accataatgg
      301 gagaaaatcc agccaaaaca atgaatgttc tttcgggttt gaacttagcc atgttaatgg
      361 aagcagtctt tgctagaatg gctcatagct ttgatgaggt tgttaataaa tcagtagtgg
      421 cggcccaggg cggagtcgta aatggtaaag aattgttttc aacggatgca gaggaagagg
      481 aagaagattt cgaatcgggt atttaaaggg taaaagaatg ataaaaaagg ttacgattga
      541 aaaaataaaa tcgcctgagc gcttcttaga agtaccactt ctgacgaaag aagaagtcgg
      601 ccaggcaatc gataaggtta ttcggcagtt agaactcaac cttgactatt tcaaggaaga
      661 tttcccgacg ccagctacct ttgataatgt ctatccaatc atggataaca cggaatggac
      721 caatggtttc tggacaggag aactgtggtt ggcttatgaa tacagtcaac aggatgcatt
      781 taaaaacatc gctcataaaa atgttctttc tttcctggat cgtgtcaata agagagtaga
      841 attggatcac catgatctcg gcttcttgta cacaccgtct tgtatggctg aatataagat
      901 aaatggagat ggagaggcta gagaagcagc cttgaaagct gcagataagt tgattgaacg
      961 ctatcaagaa aaaggtggtt ttattcaagc ttggggagac ttgggcaaga aagagcatta
     1021 ccgtttgatt atcgattgct tgctcaatat ccaactctta ttctttgctt atcaagaaac
     1081 aggcgatcaa aaatactacg atattgcaga aagccatttc tatgcttcag ctaataatgt
     1141 aatccgtgat gacgcttcgt ccttccacac cttctatttt gatcctgaga caggtcaacc
     1201 ctttaaaggt gtaacgagac aagggtatag tgatgattca tgctgggcac gtggtcagtc
     1261 atggggagtt tatggtattc ctttgactta tcgtcactta aaagacgagt cctgctttga
     1321 cttgtttaag ggtgtgacca attatttctt gaatcgtcta ccaaaagatc atgtgtccta
     1381 ttgggatttg atttttaatg atggtagtga tcaatcacga gattcttcag caacagctat
     1441 cgccgtctgt gggattcatg aaatgctaaa acatctccca gaggtggatg ctgacaaaga
     1501 tatttataaa catgctatgc atgccatgct tcgttccttg atcgaacatt atgcaaataa
     1561 tcaatttacc cctggtggga caagtctcct ccacggtgtg tactcatggc attcaggtaa
     1621 aggagtggat gaaggcaata tctggggtga ctactattac ctagaagccc ttatccgttt
     1681 ctacaaagac tggaacctat attggtagga ggagaaatat gacaatgcca aatattatta
     1741 tgacccgtat cgatgaacgg ttgattcatg gacaaggaca actttgggta aaatacctag
     1801 gttgtaatac ggtcattgtt gccaatgacg aagtaagcac ggacaagatg caacaaactc
     1861 tgatgaaaac agttgtgcca gactcagttg ccatgcgttt cttccctttg caaaaggtga
     1921 ttgatatcat tcacaaggct aatcctgctc aaacgatctt tatcgttgta aaggatgtga
     1981 aggacgcttt aaccttggta gaaggtggtg tccctatcaa agaaatcaat attgggaaca
     2041 ttcacaatgc tactggtaaa gagcaagtga cacgctccat cttcctgggt gaagaggaca
     2101 aggcggccct caaggaattg aaccaaactc atcaagtaac atttaatacg aaaacaactc
     2161 caacaggaaa tgatggagct gttcaagtca acattatgga ctatatttaa aagaggagat
     2221 cgttatgtcg attaatgtat ttcaagcgat tttaattgga ttatggacag ctttctgttt
     2281 tagtggaatg ctgttaggaa tttacaccaa tagatgcatt gttctgtcat ttggtgtcgg
     2341 aattattcta ggtgatctgc ctactgctct tgcaatggga gctattggtg aattggctta
     2401 tatgggattc ggtgttggtg ctggaggtac tgttccacca aacccaatcg gacctggtat
     2461 ctttggtacc ttgatggcta tcactagtgc tggtaaagtc agtccagaag cggctcttgc
     2521 cctctctact ccgattgctg tggcgattca attcttacaa actttcgcct acactgtacg
     2581 tgctggtgcg cctgaaacag cgatgaagca cttgaaaaac cataatttga agaaattcaa
     2641 gttcactcta aatgcaacaa tttggttgtt tgcctttatt ggatttacct tgggttgctt
     2701 gggtgccctt tcaatggata ccttgttgaa actcgtagac tacattccac cggtattgct
     2761 tacaggtttg acagttgctg gtaaaatgct cccagctatc ggttttgcga tgatcttgtc
     2821 agtgatggct aagaaagagt tgattccctt tgtcttgttg ggatatgttt gtgcagctta
     2881 tctaaacatc ccaacaattg gtattgcaat tgtaggtact atctttgctt tgattgaatt
     2941 ttataacaag ccaaaaacag cggatcatgt ggtagaggag gaagcacacg atgactggat
     3001 ctaacaaatt aacaaaacgt gattatctta aaacgtcttt gcgggcattc ttttgtcaaa
     3061 atggatttaa ctatagtaac tatcaagggt tgggatatgc caatgtgatg tatcctgctt
     3121 tgaaaaaaca ctatggagag gatcaggaag gtttctacca agccttggaa gaaaactgtg
     3181 aattctataa taccaaccca cacttcctgc cttttattac cagcttgcat cttgtaatgt
     3241 tggaaaatgg ccgtccggca aaagaaacac gtagcatcaa gatggccttg atgggaccat
     3301 tggcaggtat tggggattct ctttctcaat tctgtttagc tccattgttc tcaaccatcg
     3361 cagcttcgtt tgctcaagaa ggcttggttg tcggtccaat cttgttcttc cttgcgatga
     3421 atacgatttt aacagcgatt aaattgtcaa ctggtctgta tggatacaaa ctaggaacaa
     3481 ctgtgattga taaactaagc gaacagatgg caacgatttc tcgtattgcc aatattatcg
     3541 gtgtaaccgt aattgctggt ttggcagcga catctgttaa gattatggtg ccgattacct
     3601 ttgctgcagg ggaagttaaa gcagacgcta aacaaagtat cgtaagtatt cagggaatgc
     3661 ttgataaggt tgctccagct cttctaccag ccctatttac acttttagtt tactacttga
     3721 tcaaagaaaa gaaatggaca acatataaac tcgttatttt aacagttatc atcggaatta
     3781 tcggaagctg gcttaagatt atagcttaat ggatacaaca ttgttttatg gaatagtgat
     3841 tgtcttggca gtgagtcccc ttttactgtc aagctttcat tctattcgtc agcaaaagtt
     3901 gcttcgcaaa cagatggagc aacgacaaga gtatttagct tctttaacat ctggtgatga
     3961 agtgttgtta ttgtctggaa ttcatggaaa aatcatttct atccaagatg acttggtctc
     4021 cttgcagatt gcaaaaggtg tggtcatcta tgtagaaaag gaaagtgtaa tgggaaagac
     4081 aaaagaactg ctttttaagt agttcttttt ctggtaatag ataatgaaag aagaaagaag
     4141 acaatttttt gaaagagtcg atggaaacca atgtcgtgat tatatcttgt cccactgttc
     4201 aaaagactat gagaaagtca agagttccct tgaacgcttg atagacaatc gttttatgtt
     4261 tgatagtcct tgggatatgg agccttgttc aaaaatccat caaatccagc cgatggtatg
     4321 ggatcaagta tttgaagatg atccagaatg gtcttatatg ctcaatcgac aagaatatct
     4381 cttgcagttt atgatagggt atctggtaga aggagataag ggctatatcc aaaagtgcaa
     4441 gttctttcta tttgattgga ttgagcaggt gagagaattt tctcctcaat ccttgatgac
     4501 tagaaccttg gatacgggta ttcgttcctt tacttggttg aaactactct tgctcctctt
     4561 gaaatttgac ttgctagagg agaaagaact agagaaaatt ttggtcagtc tagaaaagca
     4621 gattgacttt atgaaaagcc actatcgcgc caagtacacc cttagtaact gggggatttt
     4681 acaaacaatt ccgatgcttg ctatctatta tttcttttca gataagatgg acctagaaga
     4741 agcttaccat tttgcttcag aggagttgaa acagcaaatt gagacacaga ttttaggaga
     4801 tggaagccag tttgaacagt cgattctcta tcatgtagag gtttataaag ccttgctgga
     4861 tttgtgtctc ttgcttccag acttgcaaga tagttaccga gagttgctgg aaaagatggc
     4921 gacctatatt caaatgatga caggcttaga tggacgaact ttggcttttg gtgatagcga
     4981 ttctacagaa acgacagaaa ttttgagcct gtctgctgtg gttttgaaca aggaagacct
     5041 tcttaacggt ctggatgtta aagtcgattt gcttagcctc ttgttcctgg ggcgagaaaa
     5101 ggtcaagcga ctgcaggaat ttgaaaggag agcttggcag cctaagtcca tgatctttga
     5161 agactctgga catgtctgca ttaaggatga acatcgttat ctatttttca aaaatggtcc
     5221 gctaggaagt gcccatagcc atagtgacga gaatagtttt tgcttacagt atcaaggcca
     5281 acctattttc atagatgctg ggcgttattc ttatcgggag atatatgaac gttatctctt
     5341 aaagagtgct tggagtcatt cgacctgcat tgtagatggg aaagctccgg aaagaatcac
     5401 gggatcctgg gaatatgaat actatcctca ctccctgttt tgtcaccata aagaaaggga
     5461 gggagtgcat tatattgagg gggcttattg gtcagcagaa cctgatttgc cttatcttca
     5521 caagagaaaa atcctcatgt tggtagagga tgtctggctc ttggtagatg acatcaggtg
     5581 tcaaggtcag catgaggcgt tgactcagtt tatccttgac aaggatgtga cctatcaaga
     5641 tgggaaaatc aatcagttga gactatggag tgaagttgat tttgatttgg aagataccat
     5701 catttctcct aaatacaatg agcttgaaag aagtagcaaa ctcaccaagc gccaattctt
     5761 tgagaatcag atgctggatt ataccatcat tgcgcatgag agttttgaaa ttatccgtca
     5821 ttctgtctac cagacagatg atcgtgaagt ggaaaatgct ctggcttttg aagtgaaaaa
     5881 tgacgaaaca gacaagctga ttctgttatt aagcgaggat attggtgtag gtgaaaaatt
     5941 gtgcctcgtt gacggaacaa aaatgcgtgg aaaatgtcta gtatatgata aaataaatga
     6001 gagaatgatt cgcttgcagt gctagaaata ggcattttga atagtgaata tgttataata
     6061 agtattagta ggaggtgttt tagattggag aagaaactga ccataaaaga cattgcggaa
     6121 atggctcaga cctcgaaaac aaccgtgtca ttttacctaa acgggaaata tgaaaaaatg
     6181 tcccaagaga cacgtgaaaa gattgaaaaa gttattcatg aaacaaatta caaaccgagc
     6241 attgttgcgc gtagcttaaa ctccaaacga acaaaattaa tcggtgtttt gatcggtgat
     6301 attaccaaca gtttctcaaa ccaaattgtt aagggaattg aggatatcgc cagccagaat
     6361 ggctaccagg taatgatagg aaatagtaat tacagccaag agagtgagga ccggtatatt
     6421 gaaagcatgc ttctcttggg agtagacggc tttattattc agccgacctc taatttccga
     6481 aaatattctc gtatcatcga tgagaaaaag aagaaaatgg tcttttttga tagtcagctc
     6541 tatgaacacc ggactagctg ggttaaaacc aataactatg atgccgttta tgacatgacc
     6601 cagtcctgta tcgaaaaagg ttatgaacat tttctcttga ttacagcgga tacgagtcgt
     6661 ttgagtactc ggattgagcg ggcaagtggt tttgtggatg ctttaacaga tgctaatatg
     6721 cgtcacgcca gtctaaccat tgaagataag catacgaatt tggaacaaat taaggaattt
     6781 ttacaaaaag aaatcgatcc cgatgaaaaa actctggtat ttatccctaa ctgttgggcc
     6841 ctacctctag tctttaccgt tatcaaagag ttgaattata acttgccaca agttgggttg
     6901 attggttttg acaatacgga gtggacttgc ttttcttctc caagtgtttc gacgctggtt
     6961 cagccctcct ttgaggaagg acaacaggct acaaagattt tgattgatca gattgaaggc
     7021 cgcaatcaag aagaaaggca acaagtcttg gattgtagtg tgaattggaa agagtcgact
     7081 ttctaaaatg aaggaaaatg acttgcaatt tctgttaaga aataaaataa tcccacctag
     7141 aacaagctag gtgggattat ttgcctatga aatgagaaat tatgggagca agctcctaaa
     7201 tcaactgttt ttgatctact tctttaacta cttgataaaa gttatagaag taggccaaac
     7261 ttgaaatgat ggttacgact aggaatattg aaaatttcca ttggacaggg ttggttaaaa
     7321 gttgtggaaa ggatatgagg agaaagaaga gggctgcgtt gaggacaggt atccgttttg
     7381 attgtatttt ctcaagtcct ttattgagcg caggaagaaa gaggagtagg agtagtaaaa
     7441 ctgtatgaga aacagctcct gaaataaggg cgaagaaaag gaaaatactg ataaaaacat
     7501 gaatgatcag tagtctagct agtgatttca taaggcacct cctaatcctg gtctttttta
     7561 gctcttgcaa tacgaagtga gtcgacaata tgtatcatca ctccgaaaaa gaaagctccc
     7621 agtatagttt taaaaatatg ttttgtattt agaagagaac tgataaaatt tggattttca
     7681 cttgttaggg tatcaatgag tggaattata aaaaatatca ctgttccata aatcgaacct
     7741 gctttcagac caggataacg taactgtttc ttttcttttt tcatgagttt gctccttttt
     7801 taaaaatctt ccttccaaaa gagactgttg aggtcagttt ggaggctgcg agcgagattg
     7861 agacagagtt ccagagttgg attgtacttg tcgttttcaa tcatattgat ggtctgtctc
     7921 gagacaccga tatccttggc gagctcgagc tgggaaatgc ccagttcctt gcgaaattct
     7981 ttcacacgat tcatctggtc tcctttctga tttgtgtcgt atatatttga ctttattata
     8041 gtcttttaaa cataaagtgt caagtatttt tgacatattt tttgaagaaa tagtagtctc
     8101 cttgtcctat ttgtctgaca agtgcaagct ggtcggattt gtggtaaaat agataagata
     8161 tgacaaaaga atttcatcat gtaacggtct tactccacga aacgattgat atgcttgacg
     8221 taaaacctga cggtatctac gttgatgcga ctttgggtgg agcaggccat agcgagtatt
     8281 tattaagtaa attaagtgaa aaaggccatc tctatgcctt tgaccaggat cagaatgcca
     8341 ttgacaatgc gcaaaaacgc ttggcacctt acattgagaa gggaatggtg acctttatca
     8401 aggacaactt ccgtcattta caggcacgtt tgcgcgaaac tggtgttcag gaaattgatg
     8461 gaatttgtta tgacttggga gtgtctagtc ctcaattaga ccagcgtgag cgtggttttt
     8521 cttataaaaa ggatgcgcca ctggacatgc ggatgaatca ggatgctagt ctgacagcct
     8581 atgaagtggt gaacaattat gactatcatg acttggttcg tattttcttc aagtatggag
     8641 aggacaaatt ctctaaacag attgcgcgta agattgagca agcgcgtgaa gtgaagccga
     8701 ttgagacaac gactgagtta gcagagatta tcaagttggt caaacctgcc aaggaactca
     8761 agaagaaggg tcatcctgct aagcagattt tccaggctat tcgaattgaa gtcaatgatg
     8821 aactgggagc ggcagatgag tccatccagc aggctatgga tatgttggct ctggatggta
     8881 gaatttcagt gattaccttt cattccttag aagaccgctt gaccaagcaa ttgttcaagg
     8941 aagcttcaac agttgaagtt ccaaaaggct tgcctttcat cccagatgat ctcaagccca
     9001 agatggaatt ggtgtcccgt aagccaatct tgccaagtgc ggaagagtta gaagccaata
     9061 accgctcgca ctcagccaag ttgcgcgtgg tcagaaaaat tcacaagtaa gagggaaaaa
     9121 gatggcagaa aaaatggaaa aaacaggtca aatactacag atgcaactta aacggttttc
     9181 gcgtgtggaa aaagcttttt acttttccat tgctgtaacc actcttattg tagccattag
     9241 tattattttt atgcagacca agctcttgca agtgcagaat gatttgacaa aaatcaatgc
     9301 gcagatagag gaaaagaaga ccgaattgga cgatgccaag caagaggtca atgaactatt
     9361 acgtgcagaa cgtttgaaag aaattgccaa ttcacacgat ttgcaattaa acaatgaaaa
     9421 tattagaata gcggagtaag atatgaagtg gacaaaaaga gtaatccgtt atgcgaccaa
     9481 aaatcggaaa tcgccggctg aaaacagacg cagagttgga aaaagtctga gtttattatc
     9541 tgtctttgtt tttgccattt ttttagtcaa ttttgcggtc attattggga caggcactcg
     9601 ctttggaaca gatttagcga aggaagctaa gaaggttcat caaaccaccc gtacagttcc
     9661 tgccaaacgt gggactattt atgaccgaaa tggagtcccg attgctgagg atgcaacctc
     9721 ctataatgtc tatgcggtca ttgatgagaa ctataagtca gcaacgggta agattcttta
     9781 cgtagaaaaa acacaattta acaaggttgc agaggtcttt cataagtatc tggacatgga
     9841 agaatcctat gtaagagagc aactctcgca acctaatctc aagcaagttt cctttggttc
     9901 gaagggcaat gggattacct atgccaatat gatgtctatc aaaaaagaat tggaaactgc
     9961 agaggtcaag gggattgatt ttacaaccag tcccaatcgt agttatccaa acggacaatt
    10021 tgcttctagt tttatcggac tagctcagct ccatgaaaat gaagatggca gcaagagctt
    10081 actgggaacc tctggaatgg agagttcctt gaacagtatt cttgcaggga cagacggtat
    10141 tattacctat gaaaaagacc gtgtaggaaa tatcgtacca ggtacagaac tggtatcgca
    10201 acaaactgtg gatggcaagg atgtttatac aacattgtct agtccgctac aatctttcat
    10261 ggaaactcag atggatgcct ttctagaaaa agtaaaaggt aagtatatga ccgcgacctt
    10321 ggtcagtgca aagaccggtg aaatcctcgc taccacccaa cgacctacct ttaatgcaga
    10381 tactaaagaa ggaatcactg aggactttgt ttggcgtgat attctttatc aaagtaacta
    10441 tgaaccagga tcagccatga aggttatgac gttagcttct tctattgata ataatacctt
    10501 cccaagtgga gaatacttca atagcagtga attcaaaata gcggatgcga cgactcgaga
    10561 ttgggatgtt aatgatggtt tgactactgg tgggatgatg actttcttac aaggtttcgc
    10621 tcactccagt aatgttggaa tgagtctact tgaacaaaaa atgggagatg ctacttggtt
    10681 ggattatcta aaacgcttta aatttggggt tccaactcgc tttggcttga cagatgaata
    10741 cgctggtcaa cttccagctg ataatattgt tagtattgct caaagctcat ttgggcaagg
    10801 aatttcagtg acacaaacac aaatgcttcg tgcctttaca gctattgcta atgatggagt
    10861 tatgctggag ccaaaattta taagtgctat ttatgatact aacaatcagt ctgtacgtaa
    10921 gtcacaaaaa gaaatagtag gaaatcctgt ttccaaagag gcagcaagca caactcgaaa
    10981 tcacatgatc ttagttggga cggaccctct atatggaact atgtataatc actacacagg
    11041 aaagccaatt ataacagttc ctggacaaaa tgtagcagtt aaatccggta cggctcaaat
    11101 cgctgatgag aaaaatggag gatacttggt tggttctacc aattatattt tctcagttgt
    11161 gactatgaat cctgctgaaa atcctgattt tatcttgtat gtaacggttc aacagcctga
    11221 gcattattca ggtatccagt tgggagaatt tgccacccca atcttggagc gggcttcagc
    11281 tatgaaagaa tctctcaatc ttcaatctcc agccaaaaat ttagataaag ttacgacaga
    11341 atcttcttat gcaatgccta gcatcaagga tatttcacct ggtgagttgg cggaagcctt
    11401 acgccgaaat attgtgcaac caatcgttgt aggtactgga acaaagatta aagagacttc
    11461 tgtagaagaa gggaccaatc ttgcaccaaa ccaacaagtt ctccttttat cggataaggt
    11521 agaagaaatt ccagacatgt atggctggaa aaaagagact gccgagacct ttgctaaatg
    11581 gttggatatt gaactggaat ttgaaggttc aggttccgtt gttcagaagc aagatgttcg
    11641 gactaataca gctatcaaaa acattaaaaa aattaaatta actttaggag actaatatgt
    11701 ttatttctat cagtgctgga gttgtgacat ttttaataac tttagtagga attccggcct
    11761 ttatccaatt ttatagaaag gcgcaaatta caggccagca gatgcatgag gatgtcaaac
    11821 agcatcaggc aaaagctggg acgccaacta tggggggact tgttttcctc attgctgcag
    11881 ttgtggtgag tttccttctt gctctttttt caaaacaatt gaccaataat gtgggaatga
    11941 ttttgttcat cttggtcttg tatggcttgg tcggattttt agatgacttc cttaaggtct
    12001 ttcgtaagat caatgagggg cttaatccca agcaaaaatt agctcttcag cttctaggtg
    12061 gagttatctt ctatcttttc tatgagcgtg gaggtgatat gctttccgtc tttagttacc
    12121 aagtgcatct agggattttc tatattatct tcgctctttt ctggctagtc ggtttttcaa
    12181 atgcagtcaa cttgacagac ggtattgacg gtctagctag tatttccgtt gtgattagct
    12241 tgtctgccta tggagttatt gcctatgtgc aaggtcagat ggatattctt ctggtgattc
    12301 tagccatgat tggtggtttg ctcagttttt tcatctttaa ccataagcct gccaaggtct
    12361 ttatgggtga tgtgggaagt ttggccctag gtgggatgct ggcagctatc tctatggctc
    12421 tccaccaaga atggactctc ttgattatcg gaattgtgta tgtttttgaa acaacttctg
    12481 ttatgatgca agtcagttat ttcaaactga caggtggtaa acgtattttc cgtatgacgc
    12541 ctgtacatca ccattttgag cttgggggat tgtctggtaa aggaaatcct tggagcgagt
    12601 ggaaggttga cttcttcttt tggggagtgg gacttctagc aagtctcctg accctagcaa
    12661 ttttatattt gatgtaagaa tggcaccctg atgtttcagg gtgtttttgt gtttaaatac
    12721 acaatgaaaa tcaaagaaca aactagaaag ctaactttag gctgctcaaa atatagtaga
    12781 ttgaaactag aatagtacac atctacttct aaaatattgt tagaaatcga tttgactgtc
    12841 ctgattgatt tgtcctgttc ttatttcatt ttaatataca gttttgaggt tgtagataag
    12901 gagaagctga tgtggtttga agaggttttc gaagagtgta aaaaaatatt ggtcagttaa
    12961 agtcaaaact cttgaccttt tctgaccaat gtagtatatt atgaacgtaa ggtaaatgaa
    13021 agccttacta gaacacttat ttaaagtaaa atagaaagag gtgggtctat gtttcatcta
    13081 gaaatcttca gaagtaaaga tagtctactc ctgcttgaaa aagaaaaacc agaaatagta
    13141 cgtagggtag cgatctagct agtctaaatt ttttaaaaca atggtcaaag atagtcaata
    13201 tcagtaatca taactaagta aacaaaaaga ggtaaagaat atgaataaca actttaataa
    13261 ttttaataac atggatgatt tatttaacca attgatgggt ggtatgcgag gatacagttc
    13321 tgaaaatcgc cgttacttaa ttaatggacg cgaagtcaca cctgaggaat ttgctcacta
    13381 tcgtgcgact ggtcaattac caggaaatgc agaaactgat gtgcaaatgc cacaacaggc
    13441 atcaggtatg aaacaagacg gtgtccttgc aaaactaggt cgaaacttaa cagcagaagc
    13501 gcgtgagggc aagttggacc ctgttatcgg acgaaacaag gaaattcaag aaacatctga
    13561 aatcctctca cgccgcacca agaacaatcc tgttttggtc ggagatgcag gtgttggtaa
    13621 gacagcagtt gtcgaaggtc tagcgcaagc cattgtgaac ggagatgttc ctgctgctat
    13681 caagaacaag gaaattattt ctattgatat ctcaggtctt gaggctggta ctcaataccg
    13741 tggtagcttt gaagaaaatg tccaaaactt agtcaatgaa gtgaaagaag cagggaatat
    13801 tatcctcttc tttgatgaaa ttcaccaaat tcttggtgct ggtagcactg gtggagacag
    13861 tggttctaaa gggcttgcgg atattctcaa gccagctctc tctcgtggag aattgacagt
    13921 gattggggca acaactcaag acgaataccg taacaccatc ttgaagaatg ctgctcttgc
    13981 tcgtcgtttc aacgaagtga aggtcaatgc tccttcggca gagaatactt ttaaaattct
    14041 tcaaggaatt cgtgacctct atcaacaaca ccacaatgtc atcttgccag acgaagtctt
    14101 gaaagcagcg gtggattatt ctgttcaata cattcctcaa cgtagcttgc cagataaggc
    14161 tattgacctt gtcgatgtaa cggctgctca cttggcggct caacatccag taacagatgt
    14221 gcatgctgtt gaacgagaaa tcgaaacgga aaaagacaag caagaaaaag cagttgaagc
    14281 agaagatttt gaagcagctc taaactataa aacacgcatt gcagaattgg aaaagaaaat
    14341 cgaaaaccac acagaagata tgaaagtgac tgcaagtgtc aacgatgtgg ctgaatctgt
    14401 ggaacgaatg acaggtatcc cagtatcgca aatgggagct tcagatatcg aacgtttgaa
    14461 agatatggct catcgcttgc aagacaaggt gattggtcaa gataaggccg tagaagctgt
    14521 agctcgtgct atccgtcgta accgtgctgg ttttgatgaa ggaaatcgcc caatcggcag
    14581 cttcctcttt gtagggtcta ctgggattgg taagacggag cttgctaagc aattggcact
    14641 cgatatgttt ggaacccagg atgcgattat ccgtttggat atgtctgaat acagtgaccg
    14701 cacagctgtt tctaagctaa ttggtacaac agcaggctat gtgggttatg atgacaatag
    14761 caatacctta acagaacgtg ttcgtcgcaa tccatactct atcattctct tggatgaaat
    14821 tgaaaaggct gatcctcaag ttattaccct tctcctccaa gttctagatg atggtcgttt
    14881 gacagatggt caaggaaata cagtaaactt caagaatact gtcgttattg cgacctcaaa
    14941 tgctggattt ggctatgaag ccaatttgac agaagatgcg gataaaccag aattgatgga
    15001 ccgtttgaaa cccttcttcc gtccagaatt cctcaaccgc tttaatgcag tcatcgagtt
    15061 ctcacacttg actaaggaag acctttctaa gattgtagat ttgatgttga ctgaagttaa
    15121 ccaaaccttg gctaagaaag acattgactt ggtagtcagt caagcggcta aagattatat
    15181 cacagaagaa ggttacgacg aagtcatggg ggttcgtcct ctccgtcgag tggttgaaca
    15241 agaaattcgt gataaggtga cagacttcca cttagatcat ttagatgcta aacatctgga
    15301 agcagatatg gaagatggcg ttttggttat tcgtgagaaa gcctaagaca gaatttttag
    15361 gataaaaaag aaggagccag ctgaaaaaaa ctggttcctt ttgaggtacg acgggcatgt
    15421 cgtgtagtag gttgaaacta gaatagtaca catctacttc taaaacattg ttagaaatcg
    15481 atttgactgt cctgaacgat ttgtcctgtt cttatttcat tttactatat atctgaatat
    15541 ctgaggtgta agtcctcggt gggcacccac taccggtgaa cccaatagcg attcccaagc
    15601 ctgactatcg tgaggtagtg gattaaaacg gtctggggat agaccgtttt aagtctgacg
    15661 ctggaaataa gaatcgt
//
LOCUS       DAVKGU010000041        15598 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKGU010000041 DAVKGU010000000
VERSION     DAVKGU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.041"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            110..2002
                     /gene="pepO"
                     /locus_tag="VW077_001213"
     CDS             110..2002
                     /gene="pepO"
                     /locus_tag="VW077_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9455235.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEERSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2125..2754)
                     /locus_tag="VW077_001214"
     CDS             complement(2125..2754)
                     /locus_tag="VW077_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9455236.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAYLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            3711..5933
                     /locus_tag="VW077_001215"
     CDS             3711..5933
                     /locus_tag="VW077_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9455237.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5963..6406
                     /locus_tag="VW077_001216"
     CDS             5963..6406
                     /locus_tag="VW077_001216"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9455238.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6643..6859)
                     /locus_tag="VW077_001217"
                     /pseudo
     CDS             complement(6643..6859)
                     /locus_tag="VW077_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7023..7673)
                     /locus_tag="VW077_001218"
     CDS             complement(7023..7673)
                     /locus_tag="VW077_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9455239.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            7785..8627
                     /locus_tag="VW077_001219"
     CDS             7785..8627
                     /locus_tag="VW077_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9455240.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            8703..9140
                     /locus_tag="VW077_001220"
     CDS             8703..9140
                     /locus_tag="VW077_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9455241.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRN
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            9208..9459
                     /locus_tag="VW077_001221"
     CDS             9208..9459
                     /locus_tag="VW077_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455242.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFTFVLLPFLLFLSGIFLIYNGVILLK"
     gene            complement(9601..10674)
                     /locus_tag="VW077_001222"
     CDS             complement(9601..10674)
                     /locus_tag="VW077_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9455243.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLELAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            10830..11507
                     /locus_tag="VW077_001223"
     CDS             10830..11507
                     /locus_tag="VW077_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9455244.1"
                     /translation="MHKILLVEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            11500..12474
                     /locus_tag="VW077_001224"
     CDS             11500..12474
                     /locus_tag="VW077_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9455245.1"
                     /translation="MLDWKQFFLAYLRSRSRLFVYLLSLAFLVLLFQFLFASLGIYFL
                     YFFLLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(12523..14466)
                     /gene="thrS"
                     /locus_tag="VW077_001225"
     CDS             complement(12523..14466)
                     /gene="thrS"
                     /locus_tag="VW077_001225"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9455246.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(14524..14700)
                     /locus_tag="VW077_001226"
     CDS             complement(14524..14700)
                     /locus_tag="VW077_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9455247.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMKNF
                     LSDKEKQLNVRTRK"
     gene            complement(14707..14847)
                     /locus_tag="VW077_001227"
     CDS             complement(14707..14847)
                     /locus_tag="VW077_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9455248.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(15278..15478)
                     /locus_tag="VW077_001228"
     CDS             complement(15278..15478)
                     /locus_tag="VW077_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455249.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIISLLYSYRFRFSL"
BASE COUNT         4471 a         2938 c         3646 g         4543 t
ORIGIN      
        1 ggaaaggttt tttataatag ctaaaaaata atagttaaat ggttattaat tgcattccct
       61 agtgattttt gttaagataa aggcaaatac aaatgaaagc gagaacaaga tgacacgtta
      121 tcaagatgat ttttatgatg ctatcaatgg agaatggcaa cagacagctg aaatcccagc
      181 agataagtct caaacaggag gttttgttga tttagaccag gaaattgaag acctgatgct
      241 ggcgacaaca gacaagtggt tagcaggtga agaagtgcct gaggatgcta tcttggaaaa
      301 ctttgtcaaa taccaccgcc tagttcgtga ttttgacaag agagaagctg acggtatcac
      361 acctgtctta ccactcctta aagaattcca agaattggaa acttttgcgg attttacagc
      421 taaactagca gagtttgagc ttgcaggaaa accaaacttc cttccttttg gtgtatcgcc
      481 agactttatg gatgctagaa tcaatgttct atgggctagc gctccaagca caatcttgcc
      541 agatacgacc tactatgcag aagaacatcc tcagcgcgaa gagctcttga ctctttggaa
      601 agaaagtagc gcaaatctcc tcaaggctta tgatttctct gatgaagaaa ttgaagactt
      661 gctagaaaaa agacttgaat tggaccgccg agttgcggca gtggtgctct ctaatgaaga
      721 acgttcagaa tatgctaaac tctatcatcc atattcttac gaagatttca agaaattcgc
      781 gcctgcccta cctttggatg acttcttcaa agcagttatt gggcaattac cagacaaggt
      841 tattgtagac gaggaacgtt tctggcaagc agcagagcaa ttttacagtg aggaagcctg
      901 gtctctcctt aaagcaacct tgattttgag tgttgtcaat ctttcaacca gctatttaac
      961 agaggatatc cgtgttttgt ctggtgccta cagccgtgcc ctttctggag ttccagaggc
     1021 aaaagataag gtcaaagcag cttatcatct agcacaggaa cctttcaagc aagccctggg
     1081 gctttggtac gcccgtgaga agttctctcc agaagccaag gcggatgtgg agaaaaaagt
     1141 ggcaaccatg attgatgtct ataaggagcg tctgcttaag aatgactggc tcactccaga
     1201 aacctgtaaa caggctatcg tgaagctcaa tgtgatcaaa ccttatattg gctatccaga
     1261 agaattgcct gcacgttaca aggataaggt agtgaatgaa actgccagtc tttttgagaa
     1321 tgctctagcc tttgcgcgtg tggaaatcaa gcacagttgg agtaagtgga accagcctgt
     1381 agactataag gaatggggca tgcctgctca tatggtcaat gcctactaca atcctcagaa
     1441 gaacctgatt gtctttccag cggccatttt acaggcgcct ttctatgact tgcatcagtc
     1501 atcttctgct aactacggtg gtattggggc agtgattgcc catgaaattt cccacgcctt
     1561 tgatactaac ggggcttcct ttgacgaaaa tggtagcctc aaggattggt ggacagagag
     1621 cgactatgct gccttcaagg agaaaacaca aaaagtcatt gaccaatttg atggacagga
     1681 ttcttatgga gcaaccatta acggtaaatt gactgtatca gaaaacgtgg ctgacttggg
     1741 aggaatcgca gcagctcttg aagcagctaa gagagaagca gacttctcag cagaagagtt
     1801 cttctacaac ttcggtcgca tctggcgcat gaaaggtcga ccagaattta tgaaactttt
     1861 ggctagcgtc gatgtgcacg caccagccaa actccgtgtc aatgtgcaag taccaaactt
     1921 cgacgatttc tttacaacct atgatgtcaa agaaggagac ggaatgtggc gttcaccaga
     1981 ggagcgcgtg attatttggt aatgcaaaaa atctagtcat cagaaaaaag gcatccaatc
     2041 acgtgtgaaa accttgatag gatgcctttt ataatagaaa gatttgctgg atagtttact
     2101 taaatttcga ttgtcatcat cttgctaaaa aaagggattg aaggcttttt cgtgagcgat
     2161 agtagtagct ggaccatgtc ctggatagac atcgtagttt ggtagggtga agagttgggt
     2221 ctggatacta tgaaggagtt gctccatgct accagtcgga aggtcggtcc gtccgatagt
     2281 ttcgcggaat agagcatctc ccgtcaagac taggtaagca tcaggaaaga ctagggaaac
     2341 accaccgata gagtgccctg gggtcggtag aaccttaaaa cgaaattcct ctagttggta
     2401 ttcttcgtga aagacaaagg tgtgttctgc aggttttgtg accacatctg ccatatcatc
     2461 gtggcgaggg agaccggaga gattatcgac aggagtgtag agccagctgg cttcgctctc
     2521 tgcgatatag acaggaggat tgccaaacgt ctcgcgaacc aagtccagac tcatgatatg
     2581 gtcataatgg gcgtgggtca agagaatagc acagatcggt ttgttgatct tctcgattgt
     2641 ctgacgaatg gcttcccaat gactaccagg atcgacgacg atgaggtgct tttcgccttc
     2701 tagataatag gtattttcat aggcaacagg attcacggtt ttatggattt tcatactagc
     2761 tccaatctca aagaatgtac taatactctt cgaaaatctc ttcaaaccgc gtcaacttta
     2821 tctgcaacct caaagcagtg ctttgaacag cctgcggcta gctttctagt ttgctctttg
     2881 attttcattg agtataaatt aagtatagca cagttaggga gaataggtaa ggatttaaaa
     2941 tgaacttaaa aatcagcatg actagatgaa gacaggctgg ttatcaattt atcaatattg
     3001 gaaaggtgtc aattgccctt cataggttgc ataaactccg tcattagctt gtttgataga
     3061 cgtgatgttt agagtgctac cgtagttggc ttctcatttg tcgctatatt cactataagt
     3121 tatcctactg ggcaggttct ggtcgttagc atagtattga acgagttttt tatagctttg
     3181 ctggctgaat aggaacaggc agcaaactcc taacattagg agagcaaaga tagaaatagc
     3241 agtttttttc ataggtattc tccttataaa ctaaaggata gaagaaagta tcttgaggat
     3301 ttttatactc ttcaaaaatc tcttcaaacc gcgtcaactt tatctgcaac ttcaaagcgg
     3361 tgctttgagt aacctgcggc tagtttccta gtttgctctt tgattttcat ttagtattaa
     3421 ttccaaaatc cagtcaaggt accagtgtat gtgctacgag ttccaccgtt tatctttcgt
     3481 attttcaatg gaattgggga agtcagaaag tctttgggtt acaatatggt ttccgttctt
     3541 ctatctctcc ttctatcacc taagtttaaa atctaacttc gtagtatatt atgggctaat
     3601 aattcagaat tatcaagcaa ttttgaatta tttttttctt attctgttcc aatctagttt
     3661 tcatattctt aaaaaagtga taaaatggta ggaagaattg gagagtagag atgccgaaag
     3721 aagtgaattt aacaggcgaa gaagttgtcg ctttaaccaa agaatattta acggaagagg
     3781 atgttcattt tgtccataag gccttggtct atgctgttga atgccacagt ggtcaatatc
     3841 gcaaatcagg cgagccttat atcattcacc ctatccaagt ggcaggtatt ttagctaagc
     3901 taaagctgga tgctgtaaca gtagcttgtg gattcttgca tgatgtggtg gaagatacag
     3961 atgcgacttt ggacgatttg gaaagagagt ttggtcctga tgtgcggatg attgttgacg
     4021 gagttaccaa gcttggcaag gtcgagtaca aatcgatcga ggagcaatta gcggaaaatc
     4081 atcgcaagat gctcatggcc atgtctgagg acatccgcgt tattttggtc aaactgtctg
     4141 accgcttgca caatatgcgg accctgaaac atcttcgaaa agacaagcag gagcgtattt
     4201 ccaaagaaac catggaaatc tatgccccac ttgcccatcg tttggggatt tccagtgtca
     4261 aatgggaatt agaagacttg tctttccgtt atctcaatcc aacggagttt tacaagatta
     4321 cccatatgat gaaggaaaag cgcagggagc gtgaggcctt ggtggatgag gtagtcacaa
     4381 aattagagga gtatacgaca gaacgtcact tgaaagggaa gatttatggt cgtcccaagc
     4441 atatttactc aattttccgc aaaatgcagg acaagagaaa acggtttgag gaaatctatg
     4501 atctgattgc tattcgttgt attttagata cccaaagtga tgtttatgcc atgcttggtt
     4561 acgtgcatga attttggaaa ccgatgccag gtcgcttcaa agactatatt gccaaccgca
     4621 aggccaatgg ttatcagtct atccatacga ctgtttatgg accaaaaggg ccgattgaat
     4681 tccagattcg aaccaaggaa atgcacgagg tggctgagta cggggttgcg gctcactggg
     4741 cttataagaa aggtataaag gggcaagtta acagcaagga atcagctatt ggaatgaact
     4801 ggatcaagga gatgatggag ctccaagacc aggctgatga tgctaaggaa tttgtggact
     4861 ctgttaagga aaactatttg gctgaggaga tttacgtttt taccccagat ggagctgtcc
     4921 gttcccttcc caaagattca ggaccgattg attttgccta cgaaatccat accaaggtcg
     4981 gtgaaaaagc aactggtgcc aaggtcaatg gccgcatggt tccactgaca accaagttaa
     5041 agacagggga tcaggttgaa attatcgcca acccgaactc ctttggacct agccgtgact
     5101 ggctcaatat ggtcaagact agcaaggcgc gcaataagat tcgccagttc tttaaaaacc
     5161 aagataagga attgtctgtc aacaagggtc gtgagatgct gatggctcag ttccaagaaa
     5221 atggctatgt ggcaaataaa tttatggaca agcgccacat ggatcaagtt ctgcaaaaga
     5281 ccagttacaa gacagaagac tccctctttg cggccattgg ttttggggaa atcggtgcga
     5341 ttaccgtctt taaccgtctg actgaaaagg aacgccgtga ggaagagcgt gccaaggcca
     5401 aggctgaggc agaggagctt gtcaaaggtg gcgaggtcaa ggttgaaaat aaagaaactc
     5461 tcaaggtcaa gcatgagggg ggagtggtta ttgaaggtgc ttctggtctc ctagtgcgga
     5521 ttgctaagtg ttgtaacccc gtgcctggtg acgatattgt tggctacatt accaagggtc
     5581 gtggtgtggc tattcaccgt gtggactgta tgaacctgcg tgcccaagaa aactacgagc
     5641 aacgtctcct tgatgtggaa tgggaagacc agtactctag ctcaaataag gagtatatgg
     5701 cccatatcga tatctacggt ctcaaccgta caggactgtt gaacgatgta ctgcaagttc
     5761 tttcaaatac aaccaagaat atttcaacgg tcaatgccca accaaccaag gatatgaagt
     5821 ttgctaatat ccatgtgtcc ttcggtattg ccaacctctc tacactgacc acggttgtcg
     5881 ataaaattaa gagtgtgcca gaagtttact ctgtcaaacg gaccaacggc tagttgtcct
     5941 agctcttact agaaaggcta ttatgaaaat cattatccaa cgggttaaaa aagcccaagt
     6001 gagtatagaa ggccagattc agggaaaaat caatcaggga cttttattgc tggttggtgt
     6061 tggaccagag gaccaagagg aagatttgga ctatgctgtg agaaaactgg tcaatatgcg
     6121 gattttttca gacgcagaag gcaagatgaa cctgtctgtc aaagatattg aaggagaaat
     6181 cctctctatt tctcagttta ccctctttgc ggatactaag aaaggcaatc gtccagcctt
     6241 tacaggggca gctaaacctg atatggcatc agacttctat gatgctttca atcaaaaatt
     6301 agcgcaagaa gtgcccgttc agacaggtat ctttggagca gatatgcagg ttgagctggt
     6361 taataacgga cctgttacca ttatcctaga tactaaaaag agataagaaa gaccaagccc
     6421 agtcggcttg gtctttctca tcgatcataa aatactccaa aaagaaatcg gttcttgata
     6481 tgcttggggg aagtctcttt tcaggctttg gcagatgcga taggaaggga tgagatgtcc
     6541 taggatgagg agagttccct gaaggaaaag tggaatatca cttaaagaca ccaaggagag
     6601 aatcatcagg ctatcccata ggagaatttg taaagcaaaa cgtcagaatc tcggtctaat
     6661 ctgggaatag agttgactaa agtggtcaca aagttggttt gaaagatagg tcaatagcac
     6721 aggatgaaag aaaatgagcc aaccactata aatcaaaatc cagtcccaag taagcccccc
     6781 ttttaaatgt caaaaatgcc agcatgattc catcaatgac aaggagttga atggttttga
     6841 tgaagatgat ttttttcatg ctaaaacctc cttttttcat ctgttagtga cgtaatgctt
     6901 tatataatta ttatacagta ttcagtcggg atatgaaaac gtttactatc gtttttgata
     6961 taaaaaatag ataagaaaac cctgaaaata aaactttcag ggttggtagg ggacttgaga
     7021 aattagttga ttttctcgac atagagttgt tttgcaatgt ccatattcac ttgtaaatcc
     7081 tcgtcgttac tgaggatagt gaaggtattg ctgaagccat caaactgctt gacttggagc
     7141 tggtcaccga tgtgaagtga gtgcttgtcc agataatgga gaatgtcaaa actatcgtgc
     7201 acccgagtca ggcggtaggc gccagcttcc ttgatatcag ctagtgggag gttattgatt
     7261 tcaacgagga gttctccctt ggcaggaata gttcctccgt gggggcaggt tttagggaaa
     7321 cctagcagtt tatctagtct ttccacgaac aggtcagaga cagtgtgttc caagacctca
     7381 gcttcctcgt gaatctggtc acttgtatag tctaaatgat gaactagaaa aacttcaatc
     7441 aagcggtgct tacgatagag ctcagagacc agtttgagac cgaggtcagt cagtagatag
     7501 ccacattcct tgtcctttag gatgagattt tcacttttca ttcgtttgat catttcagtt
     7561 acggcagggg gagagacttg catgcgagcc gcaatttcct tgttggtaat cttatgcagg
     7621 tctatgccaa tttcataaat acattttaga tagtcttctt tgtttggggt cattcgtttc
     7681 ctcttgtcta atatctttat ctagtatatc aaaaaaagct agatttctct agctgtgact
     7741 ttttatgtta tacttattgc aagagaaaaa acgaggagat ggatatgacg aaaatagctc
     7801 ttctttcaga tattcatgga aataccaccg ccttggaggc tgttttggca gatgctcagc
     7861 agctaggagt ggatgaatac tggcttttgg gagatattct catgccaggg acaggacgta
     7921 gaaggatttt ggacttgttg gatcaactac cgattacggc tagagttttg ggaaactggg
     7981 aagacagtct ttggcatggt gtccgtaagg aattggacag tactcgcccc agtcaacgct
     8041 atctcttgcg ccagtgccag tatgttttag aggaaatttc cctagaagaa attgaagtgc
     8101 tccacaatca acctcttcaa attcatcgtc agtttgggga tttgacggtg ggaattagcc
     8161 atcatctgcc tgataagaac tgggggcgag agttgattca tactggcaaa caagaggagt
     8221 ttgaccgctt ggtgactcat cctccctgtg atattgctgt ttatggacat attcaccagc
     8281 agttgcttcg ttacgggact ggtgggcaat tgattgtcaa tccgggttcg attggccaac
     8341 ctttcttttt ggacgctcag ctacggaagg acttgcgggc ccagtatatg attttggagt
     8401 ttgatgacaa gggtctggta gatatggact tccgacgggt agactacgat gtggcagcgg
     8461 aattgcagct ggctaaagac ctgagacttc cctattttga ggtttactat gaaagtctgg
     8521 tcaatgggat ccaccatact catcatcagg aatttttgag agaattggct cagaaagagg
     8581 gctgcgatcg ggagttagac gactggttga aaagtggtaa cgattgacat tacaactaaa
     8641 aagggtataa taatagaaaa agaagagtag aggcagggta gcctcgtaaa aaggagaaga
     8701 ggatgcaaat tccaagtaga tttaccattg cgactcatat gctgataatc attgccctcg
     8761 aggggaagga aagcaaggtg accagtgatt ttctggctgc tagtgtcggg gtcaatcctg
     8821 tcattatcag aaatatcttg tcccagttga agaaggcaga gctgatttca gtagcgcgtg
     8881 gaacgggcgg aacagagatt gtcaaggacc ttaaggatat tagtctttta gatgtttatc
     8941 aggcggtcga atgtcttggt aagacaggtc aactcttcag tttccatgac aatccgaatc
     9001 caaattgccc tgtaggagct catattcatg atgttttgga tcaaaaattg gagagaattc
     9061 agttgactat ggaggcagaa cttggtcaaa ccagtctaga aaaagtcgtg gccgatgcag
     9121 agagtcagat gaaggattaa gagggataaa tgccctcttt tttctttgga taattatgat
     9181 aaaatgtagc tattaaagga gtaagaaatg tatctcgtta taggaattgt attagccttt
     9241 atagtgtcat tttggaagga taatagaagt ttgtggaatc cagtattatt tttattatcc
     9301 ctcatttcaa gttattttta tctatcctac cttttttata aaaatggata cgagaatgtt
     9361 cagttagctt tttatgtatt tacctttgtc ttacttcctt ttttgctttt tctaagtggc
     9421 atctttttaa tctataatgg ggtcattttg ttgaagtgag agggacgttc caagccccat
     9481 tatctctcaa tgctctttga tttttattga ctattagatt ctatttaaaa agacttagaa
     9541 tgcacatgaa agtatgtatt ctaggtcttt tgttttgtat acatttaatt ttcaagagta
     9601 ttatctattt ttccatgcct ggtaacgagt atcaatcaat aactgggtaa ggcgacccaa
     9661 ggtttctctt tcttctggag tgagggattg agcttggaca aagagatgat gaatgtattc
     9721 aagcgccttt aaggaagcca agtcgttgat agagctaata ccagcatcaa gaatagtgcc
     9781 aaagaagagg tcgaagtaga gctgaagttc ttcgtcaggg actgtggcca cccattcttt
     9841 aaaggttgtg tctacttgct ggctatcact gttggtctta tccagttgga cgaagtgctt
     9901 gtcctcaatc tgccaactaa aggtatcgtg ctgggcgatg ccacccaggg cagtactctg
     9961 aacgatgatt tggtgagcag gaatttccag catcataccg ataatggaac cttgtggaat
    10021 gaatatcttg cttctatcca ttatcctttg ataacccgca gtctgtgtca attcttgatg
    10081 gagaccaggt gcatcaaatg tataaactgc tgtgatctga ttttgcaaac tttgctcaat
    10141 ttggctagca gcatagatag cgagatttcc tcccttggaa tgcccagcca gaataacctt
    10201 ttgcttagga tgatgggcaa aaaagttctt taaatagcga agggcgtgct tttgagcagg
    10261 aatttccttc atataggtca ggtggaaatc ttccttccag ccaatgatac tgtcatctgt
    10321 cccacgaaag acaatcagat aggtatcgag gctgacacga taagtcatag ccgcaaattg
    10381 cttttgcagt tcagggtcga tgtcgttgat aaaatgggag agtttgcaat ttttgaagcg
    10441 cttgtgttga gccaattcat ctaataattg aaggcgattt ttgctagtaa gcatggtggg
    10501 ctctcttgga acctgaggtg ctagctctaa aagacgttga ggaagtgtgg agaccagatt
    10561 atcaaaggag aggtaggtga tttctattaa ggttagaatg tctaactcat tcaagggaag
    10621 gtcgtaataa gaatcatatg cgacatcttt cagatagtca aaaatattgg ccataagagc
    10681 tcctttcttt ttatttatct tagcaaattt ccctcaaatt agctagtagc attgcctgtt
    10741 tgtactggct aaaaacaggc tatttcaaat tcagtttcag accatctggc atggaaaaat
    10801 ctgttataat aatggaaaag gagaagcgca tgcacaagat tttattagtg gaagatgatc
    10861 aggtcattcg tcaacaggtt gggaaaatgc tctctgaatg gggatttgaa gtggtcctgg
    10921 tagaagactt tatggaagtt ttgagtctat ttgttcagtc ggaacctcat ctggtcctca
    10981 tggatattgg tttgcccttg tttaatggtt atcactggtg tcaggaaatc cgcaagattt
    11041 ccaaggtacc tatcatgttt ctttcttcga gagaccaggc tatggatatt gtcatggcaa
    11101 tcaatatggg ggcggatgac tttgtgacca agccttttga ccagcaggtt cttttagcta
    11161 aggttcaggg cttgttgcgt cgttcctatg agtttgggcg tgatgagagt ttgctggaat
    11221 atgctggtgt tatcctcaat accaaatcca tggatttaca ttatcaaggg caagtcttga
    11281 atttgaccaa gaacgaattc cagattttac gcgtgttatt tgagcatgca ggcaacatcg
    11341 tagcacgtga cgacctgatg cgagaacttt ggaacagtga ctttttcatt gatgataata
    11401 ccctctctgt caatgtggct cgtttgcgta aaaagttgga ggagcaggga ttggtaggat
    11461 ttatcgagac caagaaagga atagggtacg gattgaagca tgcttgattg gaaacaattt
    11521 tttctagcct atctgcgctc ccgtagtcgt ctttttgtct atctgctttc tttggcattt
    11581 ctggtcttac tctttcagtt tttatttgcc agtctaggaa tttacttcct ctactttttc
    11641 ctcttgtgtt gctttgtaac catcttattt ttcacttggg acatattggt ggaaatgcag
    11701 gtttatcgcc aggaacttct ctatggagag agggaagcca agtctccttt ggaaatagct
    11761 ttagcagaaa aattagaagc gcgtgagatg gaactctatc agcagaggtc aaaagcagaa
    11821 agaaaactga cggatttgct ggattactat accttgtggg tccatcagat aaagaccccc
    11881 attgcagcca gtcaactctt agttgcagaa gtggtcgacc gccaactgaa gcagcagcta
    11941 gaacaggaaa ttttcaaaat cgactcctat accaacctag ttttacagta cctgcgttta
    12001 gaaagtttcc atgatgattt gctcttaaag caggttcaaa ttgaggactt ggtcaaggaa
    12061 ataattcgta aatatgctct tttctttatt caaaaaggct taaatgtcaa tctacatgac
    12121 cttgataaag aaatcgtgac ggataaaaag tggctgctag tggttattga gcaaatcatc
    12181 tcaaacagtc tcaagtacac caaggaaggt ggtctggaga tttatatgga tgaccaagag
    12241 ctttgtatca aagatacggg aatcgggata aaaaacagtg atgtcctccg agtatttgaa
    12301 cgtggctttt caggatacaa tggccgtttg acccagcagt cctctggact tggcctttat
    12361 ctatctaaga aaatttctga agaactgggg caccagattc gtatcgagtc tgaggtcgga
    12421 aaaggaacga cagtgcggat tcagtttgct caagtgaact tagtccttga gtaaaaatag
    12481 atgagaaaaa gcctccagat tggaggcttt tatgctagac tcttatttct caacgcgtga
    12541 tttgttggcg atatcagcta ggatagcttg aacaaaatta tcaactgaga cagtttgtgt
    12601 ttctttttgg ccgtagcgac gaacgttgac tgtttcgtct tccatttctt tgtctccaac
    12661 aattaattgg taaggaatct tgctggtttg tgaagcacgg atcttgaact gcattttttc
    12721 attgcgctca tctacatctg cacggacacc gcggtcacgg agtttcttgg ccacttccca
    12781 agcgtagtcc acgtgttttt cgttagatac tgggatgagg gttacttggt gtggtgccag
    12841 ccatgttggg aaggccccct tgtagttctc aatcaagata gctgtgaagc gttccatagt
    12901 tgagataact ccacggtgga tcatgactgg acggtgatct tcgccatcag ctccgatgta
    12961 tttgaggtcg aagcgttctg gcaacaagaa atcaagttgg atagtagaaa gggtttcttc
    13021 ttttccaagg gcagttttaa tctggatatc caattttggt ccgtagaagg ctgcttcacc
    13081 ttcggcttca aagtagtcca cgcccatttc atcaagagct gcacgaagca tggtttgggc
    13141 attttcccac atctcatcgt tatcaaagta cttatgagta tcttgagggt cacgaagaga
    13201 gaggcggaag cggtagtcag tcaagttgaa gtcttcataa acatcgataa tcaactgaag
    13261 ggcacgttgg aattcttctt ggatttgttc tggagtaacg aataggtgac cgtcgttgag
    13321 tgacatttca cgtacacgtt gaaggccagt gagggcacca gatttttcgt aacggtgcat
    13381 cataccgatt tcagcgatac ggattggcaa ttcacggtaa gagtgaacat ggtgtttaaa
    13441 aacttggatg tggtgcggac agttcattgg acgaaggaca aattcttccc cgtcacccat
    13501 gtccatggtt gggaacatgt cttcttggta atgatcccag tgaccagaag tcttgtaaag
    13561 ctcaacagaa gcaagtggtg gagtgtagac gtgttggtag ccagaagcca actctttgtt
    13621 tacgatgtag cgttccaatt cacgacggat agtcgcacca tttggcaacc agaatggcaa
    13681 accttgtccc acttcttgtg aaatcataaa gaggtcaagc tctttaccaa gtttacggtg
    13741 gtcacgttcc ttagcttctt cacgcatttg aaggtagttt ttcaagtctt tcttgtcaaa
    13801 ccaagctgta ccgtagatac gttgcatcat agcgttgtcg ctgtttccac gccagtacgc
    13861 accagctaca tggagaaggt ggaagatttg gatacgacct gttgatggaa cgtgaggtcc
    13921 acggcagagg tctacatatt caccctgacg atagatagtc aaaccgcctt cgtcttctga
    13981 gtgttcttca atcaattcca acttgtaagg gtcatttttg aagatttcac gtgcctcgtc
    14041 tttagtcact tcttcacgaa tagatgggaa gttttctttg acgatttttt gcatttcttc
    14101 ttcgatacga ggaaggtctt cgttagagat ttgaccagct gtgttgtcag tatcgtagta
    14161 gaaaccatct tcgatggctg gaccaactcc caagtgaatg tctgggaaaa gacgacgagc
    14221 tgcttgggcg aacaagtgag ctgctgagtg acgcaagatt ggaagggcat cttcgtgatc
    14281 aggtgtcaca atttcgatgc ttccatcttc agtgatagcg cgagtagtgt cgatgagttt
    14341 gccgttgaat ttaccagcca aggctttttt agctagggaa ttgctgatag attgggcaat
    14401 ttcaaaagtt gttacgccag attcgaattc acgaacagcg ccatctggga aagtaatgtt
    14461 aatcatgatg ttctcctttt ttattatttc atagtttcta agcattgatt ttttatcagc
    14521 agactacttc cgagtcctaa cgttcaattg cttttcttta tcagagagga agtttttcat
    14581 ttccgtcaga atgtctatat ccaaacgttg agaaagttct aatagccacc agatattttc
    14641 tgaaagtttt tgttccagtg tgtagggtgt ttcatcatag tagcgtcctt gctttgtcat
    14701 caccagtcat tggaaatttc caatatcatt agataaagcc aagaggtctt cttctaccgt
    14761 ccacttgaaa tcatgatgct taacttccag ttcgtgataa gcttgtcgga ttgcccaact
    14821 gcgctccact aattcttgca actccataat tagtatccta ataaaaaaat gcgacatcct
    14881 cgaaaggacg tcgcaacgtg gttccacctt catttatgta cctcaaaaga gagggacacc
    14941 tctgatcggc tctaacgtgg ccaccgtttt atgttttcat aaaaaactca agagtagtat
    15001 cagtttagac ttcctatgcg tttccagcaa ccacgcactc tctagtagaa agggactaag
    15061 tgacttgtct ctatggatta ttatagcatg attgtgagat ttttcaagga ttttgtgaaa
    15121 gttttttgtt tttcattttt tgtagaatat gcttgatgct aataccagaa tccatgccga
    15181 ccagagcaac tgtttcatag attagcaagt aaattagtat aaactcacca aaggttatga
    15241 tagtaaagta aaagagcagt ccgttaccaa ctaaccatca aagtgagaaa cggaaacggt
    15301 agctatagag taacgatata atcaagatta ccagaggtgt aaagaagaga agattgttta
    15361 tatagagttc cttgagtgta agggggtcag aaacaactag taagaacaac tcataaagtg
    15421 gccaataaaa gaataatatg accagatagt aggggagatg ggaatagaat ctacgcatga
    15481 taatcacctc gttttaaaaa aacagacaac ttagatctct tgctatgatt attatagcat
    15541 gtttaaaaca agaaagtaaa tttgttgaca aagaagattt ttcttttcac tagaatca
//
LOCUS       DAVKGU010000042        14983 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKGU010000042 DAVKGU010000000
VERSION     DAVKGU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.042"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..198
                     /locus_tag="VW077_001229"
     CDS             <1..198
                     /locus_tag="VW077_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455250.1"
                     /translation="NTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDLLADD
                     SLAGDIILLWRIQFGTFTTET"
     gene            677..889
                     /locus_tag="VW077_001230"
     CDS             677..889
                     /locus_tag="VW077_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455251.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            972..1775
                     /locus_tag="VW077_001231"
     CDS             972..1775
                     /locus_tag="VW077_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9455252.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITINSIEVVKDYDFKS"
     gene            complement(1832..3373)
                     /locus_tag="VW077_001232"
     CDS             complement(1832..3373)
                     /locus_tag="VW077_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9455253.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(3375..3857)
                     /locus_tag="VW077_001233"
     CDS             complement(3375..3857)
                     /locus_tag="VW077_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9455254.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(3857..4943)
                     /gene="rlmN"
                     /locus_tag="VW077_001234"
                     /pseudo
     CDS             complement(3857..4943)
                     /gene="rlmN"
                     /locus_tag="VW077_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
     gene            complement(4964..5494)
                     /locus_tag="VW077_001235"
     CDS             complement(4964..5494)
                     /locus_tag="VW077_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9455255.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(5651..6256)
                     /locus_tag="VW077_001236"
     CDS             complement(5651..6256)
                     /locus_tag="VW077_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9455256.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(6429..7466)
                     /locus_tag="VW077_001237"
     CDS             complement(6429..7466)
                     /locus_tag="VW077_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9455257.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(7500..8435)
                     /locus_tag="VW077_001238"
     CDS             complement(7500..8435)
                     /locus_tag="VW077_001238"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9455258.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(8988..10178)
                     /locus_tag="VW077_001239"
     CDS             complement(8988..10178)
                     /locus_tag="VW077_001239"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9455259.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(10393..11736)
                     /locus_tag="VW077_001240"
     CDS             complement(10393..11736)
                     /locus_tag="VW077_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9455260.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYWLTQLMQPYLAMIFVNTKTRADELHSYLTSQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(11876..12691)
                     /locus_tag="VW077_001241"
     CDS             complement(11876..12691)
                     /locus_tag="VW077_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9455261.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            complement(12743..14923)
                     /locus_tag="VW077_001242"
     CDS             complement(12743..14923)
                     /locus_tag="VW077_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9455262.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGGIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
BASE COUNT         4468 a         3286 c         2768 g         4461 t
ORIGIN      
        1 aatactattt tagaaaaatt ttataaagaa caccaagtca aacccttcat atcgcctgaa
       61 cgagatttgg atacttggct cctaagtccc aagcccgttc ccaagcgaaa tatggactta
      121 ttagcagatg attcactagc aggagatatc attttactat ggagaatcca gtttggtact
      181 tttaccactg aaacatgatt ttaaaaactc ccattcgaat atgattcaaa attcgcttag
      241 aatcaatgtt ttcccacttt ttcaaaaaat aaattttcat ctaatttaaa taactttcaa
      301 ttaagtgtga attttgctta aaatcattaa aaaacacccc atggtgtgaa ccatgaagtg
      361 ttgcaaatgc tgtgttgtag ctagatctta acgtttagag aactggctag ctttacgagc
      421 tttcttaaga tctggtttcc aaaatacttt gagtacactt atggtattga tgcccctaaa
      481 catctaaaaa ctctggtaga aaaagggtat gctcttgtcg aaacttcttt tgactcactt
      541 gaccatctaa atactaccct gaaaaagaat atcctgaaga agaagggagt tactggactt
      601 tctaaaatga aatcttgcga cctggatcaa gcccttcatg agcatttttc agaagaagaa
      661 ttagctggtc actttcatgt ccttctatgg acttttttac aatggcattg ctatcacacc
      721 caatacctat ctaagcgcct ggttcgtaaa ctttattgca gctcttcctc taaatttcct
      781 aattgttgaa ccaattgccc gttttatact aagttctttt cagaaaccat ttactgggga
      841 agaagttgaa gattttcaag atgatgacga aatcccaact attatctaat ccagttctgt
      901 aaactactaa tatttgaaat ccacttcctt ttagggtaca atggtataaa tgaatttttg
      961 agaggatcag aatgaaaaaa ctagcaaccc ttcttttact gtctactgta gccctagctg
     1021 ggtgtagcag cgtccaacgc agtctgcgtg gtgatgatta tgttgattcc agtcttgctg
     1081 ctgaagaaag ttccaaagta gctgcccaat ctgccaagga gttaaacgat gctttaacaa
     1141 acgaaaacgc caatttccca caactatcta aggaagttgc tgaagatgaa gccgaagtga
     1201 ttctccacac aagccaaggt gatattcgca ttaaactctt ccctaaactc gctcctctag
     1261 cggttgaaaa tttcctcact cacgccaaag aaggctacta taacggtatt accttccacc
     1321 gtgtcatcga tggctttatg gtccaaactg gagatccaaa aggggacggt acaggtggtc
     1381 agtccatctg gcatgacaag gataagacta aagacaaagg aactggtttc aagaacgaga
     1441 ttactcctta tttgtataac atccgtggtg ctcttgctat ggctaatact ggtcaaccaa
     1501 acaccaatgg cagccagttc ttcatcaacc aaaactctac agatacctct tctaaactcc
     1561 ctacaagcaa gtatccacag aaaattatcg aagcctacaa agaaggtgga aaccctagtc
     1621 tagatggcaa acacccagtc tttggtcaag tgattgacgg tatggatgtt gtggataaga
     1681 ttgctaaagc cgaaaaagat gaaaaagaca agccaactac tgctatcaca atcaacagca
     1741 tcgaagtggt gaaagactac gattttaaat cttaaaaacc aaaaaaaata cagtatccac
     1801 attcggtact gtatttcttt tactctcatt cttaagttaa attattaaaa tcccatattt
     1861 ggtctatcca accttcataa aagtctggct catggcagac cataaggata gatcccctgt
     1921 attctttgag agcgcgtttg agctcatcct ttgcatccac atccaaatgg ttggtcggct
     1981 cgtccagcac taaaacgttg ttttcacgat tcatcaagag acagaaacga accttggctt
     2041 gctctccccc tgataatact tgaatctggc tttcaatatg tttggttgtc aaaccacaac
     2101 gggcaagggc tgcacggact tctgcttgat taagggcagg aaaggcattc cagacagctt
     2161 caagaggagt ttggcgatta ccaccttcta cttcctgctc aaaataacca agttctaaat
     2221 aatctccacg ctccacttcc ccagcgattg gcgagataat gcccaagaga ctcttcaaga
     2281 gagttgtttt tccaatacca ttagcaccaa taatcgcaac cttttgattg cgttcgaagg
     2341 taagatttaa aggcttagta agaggacggt cgtaaccaat ttgcaagttc ttggcttgga
     2401 agataaagcg ccctggtgta cgagctggtt tgaaatcaaa agatggtttt ggtttctcac
     2461 tttggagttc gataatatcc atcttatcca atttcttttg acgagacata gccatattac
     2521 gagttgcaac acgggcttta ttacgagcca caaagtcctt gaggtctgca atctctttct
     2581 gctggcgttc gtaggctgcc tctagctgag atttcttcat agcataaact tcttggaact
     2641 ggtagtagtc accagagtaa cgcgtcagct gttgattttc cacatgatag acaatattga
     2701 taacgtcatt gaggaatgga atatcgtgcg aaatgagaac aaaggcattc tcatagtttt
     2761 ggagatagcg cttgagccaa tcaatatgct cagcatccaa gtagttggtc ggctcgtcca
     2821 acagcaaaat atcaggcttt tcaaggagaa gttttgccaa aagcaccttg gttctttgcc
     2881 cacctgacaa agaagttaca tccgtatcca tgccaaagtc cataacacca agagcacgcg
     2941 ctacttcgtc aatcttagca tccaaggtat agaaatcacg actctccaga cggtcttgaa
     3001 gttctcctac ttcttccatg agagcatcaa catccgcgcc gtcttcagcc attttcatat
     3061 agaggtcatt gatacgagct tcagctttga aaagctcatc aaaagccgta cggagaacat
     3121 cacgcaccga ctgtctttca gcaaggacag agtgctgatc caagtaacca gccgtcacat
     3181 atttggacca ctcaaccttt ccttcatctg gcagcatttt accagtcacg atactcataa
     3241 aggttgattt tccttcacca ttggcaccga ccaggccgat atgttctccc ttgaggagac
     3301 ggaaggacac atcttcaaaa attgcacggt caccaaaacc gtgactcaga tttttaactt
     3361 ctaaaatact cattttaatt ccttatcttg tttttatgta atcgtttata aaggagccaa
     3421 gccagatagc cacccaaagt gttggtccac aaatcatcaa tctcaaagac acgattgaaa
     3481 tcaaagaaaa agtccaagat taattgcgta cactcgattc caagactcac aagaaaacta
     3541 aaaagaagga ccttttttgt tttccgcaaa tttggaaata gataaaggag ttggaaaatc
     3601 agaggaaaaa acaagaagac attgaggata ttttgtaaaa aaatccaaca taattgtcca
     3661 atgtcactca cttcgcccag tttccagaga gaattgaaag gagtcaaaag aaaaaccagg
     3721 cgtccaagat gctgaatatc tggagttccc actcccacgg tagattgttc ttgaggagta
     3781 aagcaaaaac agacaatgca aatgctatag aaaatgactc cccagaccaa aatatgatta
     3841 taagtcttct tcatcattaa ggatttaccg ctgcgactgc cttctggcgg tcacgtttca
     3901 ttgtgttaga gcgcaattgt ccacaagctg cgtcaatatc tgtaccatgc tcttgacgaa
     3961 ccacacagtt gacccctttt ttcttaagcg tatcatagaa agccaacacg cgctctttgg
     4021 gactacggct atattggtca tgctcactaa ctgggttata aggaatcaag tttgcataag
     4081 acaatttctt gatgttcttg agcaattcag tcaattccaa ggcttgttct acactgtcgt
     4141 tgacttcatt aagcatgata tattcaaagg ttacacgacg gtttgttgtc tcaatgtagt
     4201 attcaatagc agcaaagagt ttttcaatcg gaaaggcacg gttaatcttc atgatacttg
     4261 aacgaagttc attgttgggt gcgtgaagag acacggcaag attgacctga accccttcat
     4321 cagcaaagtc acgaatttta tgagccaaac ctgaggttga aaccgtgatg tgacgagcac
     4381 cgatagccat tcctttatca tcattgatag tacgaaagaa attcaagaca ttgttgtaat
     4441 tatcaaaggg ctcaccgatt cccatgacaa cgatatggct gacgcgttca tcctgaccac
     4501 ggctcatcaa agtatttctg aaccagcatg atttgcgcta cgatttcacc gttattgagg
     4561 tcacgttgct tcttaatcaa accagaggca cagaaggtac aaccgatatt acagccgacc
     4621 tgagtggtca cacagacaga taaaccatag tgttgacgca tgagtacagt ctcaattaac
     4681 ataccgtcgg gcaattcaaa gagatatttg actgtaccat cagcagactc ttgcacaata
     4741 ccttgtttca agggattgac cacaaactgg tcattgagct tagcaatcaa atccttggaa
     4801 aggttggtca tttcttcaaa tgactgcaca cgtttacggt agagccattc ccagatttga
     4861 tctgcacgga atttcttttc tccctgctcc aatacccatt cctgcatggt ttgatgtgcc
     4921 aaactatgaa ttgagggttt catttcttct ccttattctc tactcacttc tgacgaatga
     4981 caaaatgacg ttgtcccttg tcctctttct gagaatgctg gtctttctga cgacgtctat
     5041 tcttcttatc tgcattcgac tttcgtttag tttgagtcgg tttctttcct tttctagaag
     5101 gtgtttcttc ttccgtctta cgcattttct tgtcaaatga tgctcgctta ggggcttcat
     5161 tttctaagac aaaataggca caaccataac tacaatactc taaaaggtag tcttgtaaac
     5221 gactgatttt ttcaagtttt tcttctgttc ggtcatcctt gtaaaaacct cgtaggcgaa
     5281 gctgttcgtt gctccagtcc cccacgatat aatcaaactt ggttaatact tctgaaaaac
     5341 gctgattaaa agtcgtcaca tcaaaggcat ccttgatatt ttcaaccaag gaaaaagcta
     5401 tcccttccgt ttcgaccttg tccccgtgta aatggaactc cggaccagga aacttgttat
     5461 agttgtataa ttcaggtgca atttcttttc gcatagatat cctttttcca cgattactta
     5521 atactttatt ctaccataat ttctagcagt tagcacgttt ctcataaaaa tgaaaaaagt
     5581 ctgacgattt tgtcagacca gaatcttata acctaaaaaa gagaagaaca attcttccct
     5641 ccaactatca ttatttagca gctgcgtaca attcatctac tttattccag ttgattactg
     5701 aaaagaaagc tttgatatag tcaggacgca cgttgcggta tttcacgtag taagcatgtt
     5761 cccaaacgtc caagcccaag attggttttt taccttctga gattggtgtg tcttggtttg
     5821 ctgttgaagt cacttcaagt ttcccttctt tgttgacaac caaccatgcc caacctgaac
     5881 caaaacgagt tgttgctgct gcagtgaagg ctgcttggaa ttcttcaaat gaaccaaatg
     5941 ttgcatcgat tgctgctgcc agttctgctg aaggagctgt tttctcggga gtcatcaatt
     6001 cccagaaaag agcgtggttc aagtgtccgc caccattgtt gataagtgct tgacggatat
     6061 cagctgggat agattctaca tcagcaagca aggcttcaag gtcttcaccg atttcagggt
     6121 gtttttctaa agctgcattg gcattgttga cataagtttg atggtgtttg tcatggtgca
     6181 aatgcattgt ttccgcatcg atgtatggtt ccaaagcgtc gtatgcatat ggaagttctg
     6241 gtaagataat agccatctgt aatacctctt tttctttcta tatgaaaatg ataacgcaaa
     6301 cattcacttt tttcaagttt tttgcttata gataaggaaa tcactgacat actttctaat
     6361 aaaataagca aatcagcaag aaacccacga caagataatc ctgacgcggg ttgtagtttc
     6421 aacaaatgtc aattgacctg actagcaatc tgtaagagtg ccttttcaaa aaggaaacct
     6481 ttttcataaa gacctgtctt aatctgatag tctgtctcaa tcaaatagga aatagcttgc
     6541 ttcagaaagc tcaaagaaag tcctctcgaa tctcttaatg caaacttgat ttgataagga
     6601 tttgggttac gtcccagata actacctaaa ctacttgcaa tctgcgattc tgtttggcca
     6661 gactccgcca aaatcttcac ctgagtaaaa gtccgaaatt gtcccagcat gactgcaatc
     6721 agtttgattt catcttcccc ttgcaaggtc aagtctctca ccaaatcgcg cgcctgatcc
     6781 atctttttag tcagaataaa ctgagttaaa tcaaaaatat tgtcctgcaa ggtcttggga
     6841 attgcgttaa caatatcctc ttcctcgata acagaatccg ccttatagga ctgtaaaaag
     6901 agaagatttt tctggatttc gctaaattga aaccccgact tgatgaggag attttcaaaa
     6961 gaatgattgg taaactgcag accttgtttc tgactccact tttggaaata ctggcgcaat
     7021 tcttgttctt ttacttctac tgcatcgaag accttggcat cacgcttaag taatttgact
     7081 aaccgtcttt tgctatccag ctttccttct gcaaagatta tcaacttagt tgttggagaa
     7141 ggattgtcaa ggtattcctc aaatgactta agctcatcat ctgtcaaaaa gcgtttctta
     7201 gcagtcgtga tatccataaa ataatctaat atcacgattt tctcatccgc aaagaaagga
     7261 aggctgacca actccagttc cacatccttg taaactactt ctttcatatc aaagtaggca
     7321 aagttgaggt cagcagaatc atacccaatc tgtttcaaca tttgactctt catcacttca
     7381 aactgaccct gatctgtccc tgtaaatagg ctcaggctcg gtaaatttga taaagtcaac
     7441 ttctgacttt cttcaatggc tagcatcgac tctcctttct tcagattttt cgatttaatt
     7501 tagtcaatat agcgcaattt cccacggaaa tcttctaagc tctcgtagcc tttttccacc
     7561 atgattgctt tcagttcatt ggtaatgcgg tcaaaagcac tgacgccttc tttgtgaagg
     7621 gtcgttccca cctgcaccat acttgctcca cagaggatgt gttcaaaggc atctcgacca
     7681 gtcagaacgc cacctgttcc gataatttgg atttgaggat ttaaacgttg ataaaaggcg
     7741 tgaacattgg ctagagcagt cggtttgatg tattctccac caattccacc aaaaccattc
     7801 ttaggccgaa taacgacaga ttcgtcttct atatagaggc cgtttccgat agagttaacg
     7861 cagttgacaa acttgagcgg atatttgttg aaaatagctg ccgcttgatc aaagtgaaca
     7921 atatcaaaat aaggtggcaa tttaattcca agaggtttgg tgaagtaagc aaacacttct
     7981 gccaaaatcc ggtctgttgt ctcaaaatca taggcaatct gaggtttacc tggaacattt
     8041 ggacaggaaa gatttagctc agtcagacca cgaaaatcac tctcttggac ttttttcaaa
     8101 atagtatggg tttcctctgg agacatgccg accagagata agaagaaagt tcggttcgac
     8161 tctttttcct gcaaatctaa aagataatcc aaataatagt ctaagccatt atttggcaag
     8221 cccatagagt tgatggaacc aagtggaaca tcttggtagc gtggctcagg attcccctga
     8281 cggaagtcca aggtcgctgt cttagtaaca aaggttcctg ccgctgagtt tttgacctct
     8341 tctaactcct ctatcgtcat acaagccaca cctgctgcat tcatcaagca attgtcaaac
     8401 tcaaaaccag caatttgtgt tttcgttgat accatgattc taatcctcca gatttctttt
     8461 attgctaata ttataccata attttaattc taggtttact agattctgtg gttctatcta
     8521 tttattcgga aaagaaagtc tagtacagaa gtcatgctat ggaaacagct agtgtattag
     8581 aagtttgaac aaggcttggg taacagactt ttgaatgaag tccaagcaac aaggagaaat
     8641 ctcaaccatg agtccattcc atgactctgt tgctctagaa aaccttgcaa tagtcaagtg
     8701 ataacgaata aaactttgga ttgaaatatc cccaagatta tctattacga ggagaaatct
     8761 taacctttat catttttaaa gaaatatagt aaaatgaaac aagaacagga gaaatcgatc
     8821 aggacagtca aattgattgc taacaatgtt ttagaagttg aggtgtacta ttctagtttc
     8881 aatctactat acatgtaaat ctctactttc gcaaaatgga ttttcagaac agtatcccag
     8941 tatagttaaa aactataaaa aagagaggac gttcccctct taaaatctta ttttactgct
     9001 tctttcaaag catctacctt atccaaacgt tcccatggaa gatcaatatc tgtacgtccc
     9061 atgtgaccgt aagccgatgt ttgacggtaa attggacgct tgaggtccag catttggata
     9121 atccctgcag ggcgaaggtc aaagatttga cgagccgctt tttcaagttg actttcagct
     9181 actgttcctg taccgaaagt atcgatacga acagaaacag gttgcgcaac accgatagca
     9241 taggccaact gcacttctgc cttcttagca aggcctgctg caacgatatt cttggcaata
     9301 tagcgagccg cataagaggc tgaacgatcc accttagtcg catctttacc agagaaggca
     9361 ccaccaccat gacgagagta gccaccataa gtatctacaa taatcttacg accagtcaaa
     9421 cctgagtccc cttgaggacc accgattaca aaacgacctg tcggattgat aaagaatttt
     9481 gtcttatcat caagataaga agatggaata acttctttga tgaccttgtc aatcacatct
     9541 tgatggattt gttcattagt ggcctctgga tcatgctgag tagaaataac gactgtatct
     9601 acacgtaccg gacggtcatt ttcatcgtac tcaactgtaa cttgtgattt tgcatctgga
     9661 cggagatagc taatttctcc agacttacga agttctgcca gacgacgaac caatttatga
     9721 ctgagtgcaa ttggcaatgg cataagctct tctgtttcat ctactgcaaa tccaaacatg
     9781 agcccttggt cacctgctcc aatcaagtcc agtggatctt gatcagcatt tccacgaacc
     9841 tccaaggctt cgttaacacc ttgagcgatg tcaggagatt gttccaccaa agatgggtgt
     9901 actcccaccg tctcagcaga aaatccatat tctgtattgg tataaccaat ctctgcaatg
     9961 gtatcacgaa ccacacggtt aatatccaca taggcatttg tagaaatttc accaaaaacg
    10021 tggacagaac cagtatatac agctgtttca gcagcaacgt gcgcctctgg atcctttgct
    10081 aaaatagcat ccaaaatcgc atctgaaatt tggtctgcaa tcttatccgg atgcccctca
    10141 gatacagatt cagacgtgaa taatttacgt tctgacataa aaatgtcccc ccttaaaaaa
    10201 tattgttata gagttacaaa gtactgatag gaaaactctt cgtaataaat acctaccatc
    10261 ttatcgggac tatataactt tatcattata ccatttttaa tcgcaatttg cagtctttaa
    10321 tgtagtgttt cggtttagat agttcgttct cataaagcaa aaagttggag ctcagtactc
    10381 caacaatttc tattaaaatg tttgacgttt agctttacgc tctgcacgac cgcgagcgcg
    10441 attttcagca cgcttggttt tacggcgctt ttcatcaacc gcccattgaa ttttcttctt
    10501 ataacccggt ttgacttttt tctttttctt tttaaccaaa ccaatcattt cgatatcaag
    10561 tttatcttgt tttttctcac ggttggcacg acgatcacgg tcataggtat cttgaaattc
    10621 cccgtctttg accatcttag gactaaactt gattcccaat ttctccaact cacggatatc
    10681 cgagtcatca cttggctgat aaagggtaat agctgtacct ggtaggccat ttcgtccagt
    10741 acgaccaaca cgatgaacaa aaaaagataa gtcttgcgga atggcatcat tgatgacatg
    10801 gctgacacct tcaatgtcaa tcccacgcgc tgctaaatct gttgcgacaa tatactcaaa
    10861 atccagattt tgcacctgat tcatgattcg cttgcgttca cgaggggcaa tatcgccatg
    10921 gatttttgca accttcaaac cttgagaagt cagatatgaa tgcaattcat cagcacgcgt
    10981 tttagtgtta acaaaaatca ttgccaaata cggctgcatc aactgagtca accagtaaat
    11041 ttgagcattc ttatcatgtc ccttggtcga aatcaaccaa ttatcaatgg tgtcagaaat
    11101 aaccgtttta gtcttaattt tctccataac aggatttgat aagtattttt tcaagaatgg
    11161 ttgcagtttt tgtgggatag tcgctgagaa gaccatgaat tgcaagtctt ttggaagact
    11221 gccagcaatc ttatcaacag tttccaagaa tcccatatcc aaggtcatat ctgcttcatc
    11281 aacaacaaat gtcttggctt tatgaatagc taaatcacca gatttaacca agtcgtagat
    11341 acggcctggt gttccaataa caatatgagg ctgattgctt gccaatttct caatctggcg
    11401 agccttatcc gtaccaccca cataattaac cacacgaact tcgacatctg agtgagctga
    11461 aatctgacgc gctacttggt aaatttgagt agccaactca cgactcggtg cagtaatcac
    11521 tgcttgtaca ctatcgctag cttcatctaa ttgctggaaa atcggtaaca agaaagtatg
    11581 agtcttacct gaacctgttt ttgattctcc tactaggtca cgacctgcca aaacaatagg
    11641 aatcaacttg tcttgcacct ctgttggagt tgtaaatttt aactccttca aggcttctct
    11701 aatatagttt ttaaattgaa atttcgtaaa tgacataaca tcctcgattc tatctatctt
    11761 atcaattata ccatatttta ttccattaca gtagtctcac ttatttaggc tatttccagt
    11821 agcttctcta gtaagaaaag gctggaattt tatagttcca acctcttttc agttattatt
    11881 tccagtttaa tatagcattc aagccatagt gatcactcac ttgtggactc ttgttaccat
    11941 caaatacgac atgtaaattt tccaccgcta actctttggt agtaaagaca taatcgattc
    12001 gaaggggttc agtgttccct ttccagccat caatttcagg cggaacagta tagctaccac
    12061 ttttctcttg agcaacttca aatgcgtctt gtaagcctaa tggactagct aaaatagctt
    12121 ggtaaccttc ctgacctgct gggttgttaa aatctccagc tagtaaaagt ggcttgttca
    12181 attttttcaa gacagcctca aatcgtgccc attcttcttg gaaaccttta tcccaccaag
    12241 agagatgaac actggcaact gctagctcct tgccatcgac tacagtttca gctagggcaa
    12301 cacggcgagt atgatagtct gttggatcat ccacatctga aaccaaaatt tctctggctt
    12361 caataggtgt tttagacaag atagccacac cttcgtggta gcggtcatag ccgatatggt
    12421 tataagccca ggtccagtag taatttttcc cttgctctaa caacttttca accaagagtc
    12481 taacataatg gtcttggtga ataggctcag ctgctggcaa agcttgataa aggtcattaa
    12541 cctccacctc tgacgaggtc atctcctgat tgatttcttg gaaacaaatc aaatcatagt
    12601 ccttttcaag aatatcttca agcaaaatct ggaatttttc ctctgcttct ttctccatcc
    12661 aactgtgagt attgagtgtt aaaaatttca ttcttttctc ccaaaataag aggttggaat
    12721 acagcttcca acctcaagta tattacaatt ctactttagc aactgctgtt ttagctgcaa
    12781 gagaacctgt tttttctaat ttaactgatt taattgcatc accatttgtg aagacaacta
    12841 ctgttgaagt ttcacgtcct gctgcacgga tagcatccaa gtcagctgtg acaaggagat
    12901 ctcctgctgc aactttttgt ccttcagcaa catgaactgt aaatggttta ccttcaagac
    12961 ttactgtgtc caaaccaatg tgaaccaata cttcaagacc tgcttccgtc acaataccaa
    13021 aagcatgttt tgttgggaag atgcttgaca cagtacctga aactggagat acaatgtttc
    13081 catttgcagg ttctactgca aatccatcac ccatcatttt ttgagcaaat actggatcct
    13141 ttacttgttc caaaccaaca acttgaccgt ctgctactga gtaaacttcc tcagtaagat
    13201 ctttgaagtg aacagtgttt tgttgtgctt cagtcatttg gcttggaaga gtttcaggaa
    13261 tgatttcacc tgaatcaagg atatcttgga tatcagattt caaaatgtca gcttttggac
    13321 cgtagatagc ttgaacccct tgtcctttca tcacaagacc catagctcct tctgctttcc
    13381 attgctctgc atttcctact ttatctgcat ctttaacagt tacacgaaga cgagtcatac
    13441 atgcatcaac atcaacgatg tttacacgtc caccaagaag gttgataatg tttacagctt
    13501 gagagcctgc tgcaactttc acttcgctgc tggtttcttc tgaaccttca gcagtttcgt
    13561 agtttccgtt gcgccctgga gttgcgtagt tgaatttttg aatcatgaag tttgcgataa
    13621 agtacatgat tacagcaaag agaacagtta cccaaacgaa gttaacgata tccataccaa
    13681 taccagcact gattgcaata ggtgtacgag tcaagaactc gattgaaccg aatgagtgca
    13741 tacgtaggtt tacgacgtca gccatagcga aggcagcacc ttgaacaagt gagtaaacaa
    13801 gatacatagg tgttgcgatg aacatgaaca tgtattcgat tggttcagta acccctgtca
    13861 agaatgttgc aagagctgtt gcaatcatca tacctttgta tttatgtttc ttgtcagcat
    13921 caacattacg gtagatagca acaatcacac ccatcaagat accgaatgaa ccgatcattt
    13981 gtccaacttt gaaacgagct ggatgtactg tatctaacaa gtgttgatat tgactagcat
    14041 cagtaccttt aaggtttaca aggtctgtta cccatgcaag ccatagtggg tcttgaccga
    14101 atacttgagt acctttagct gcaccagtta aaatgtcata agtaccacca agagctgtgt
    14161 agttcattgg gatagtcaac atgtggtgaa gaccaaatgg caagagcaaa cgttccaaag
    14221 taccatacaa gaatggtgca agaattggag cagtttcttg tgagttggca atccagatac
    14281 cgaagttatt gatacctgtt tgaactactg gccagaaagc agcaagtaga attgcagcga
    14341 ttgctgaacg aagaataact acaaatggta cgaaacgttt cccgttgaag aatgaaagtg
    14401 catcaggaag tttacggaag ttgtagtatt tgttgtaagc agttgcccct acaaaacctg
    14461 agataatccc tacgaatacc cccatgttca aggctggagc ttcaagaaca ctgataaagt
    14521 aatcagcaac tttgattgaa ccaccaaaga aagtagttac catagcatct ggatttttca
    14581 acatatcgcc tgatacacca aagattgtac cagtgatacg gttaatcaag atgaaggcaa
    14641 gaccagcggc gaaagcacca ccagcacgtt ctttagccca gcttcctcca atggctaggg
    14701 caaacaaaat gtgaaggtta ccgatacccc cccaaccgat ttgctcaaga attccacctg
    14761 tgatgacaag tggtgtaaag gttgggttaa tcatcacgat agacttaccg attgaaatca
    14821 tcaaaccagc agccggcata accgcgataa ctaccatcaa agccttaccg aatttttgcc
    14881 aaaattcgaa agacaagaca tttttgaatg tatctttcat cattcaaatc tcctttattt
    14941 ttatttaggc aaacgtttta cgaaaacgtt tgcaccttct ata
//
LOCUS       DAVKGU010000043        14930 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKGU010000043 DAVKGU010000000
VERSION     DAVKGU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14930
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.043"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            239..586
                     /gene="ssrA"
                     /locus_tag="VW077_001243"
     tmRNA           239..586
                     /gene="ssrA"
                     /locus_tag="VW077_001243"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(759..846)
                     /locus_tag="VW077_001244"
     tRNA            complement(759..846)
                     /locus_tag="VW077_001244"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(810..812),aa:Ser,seq:gga)
     gene            1009..1749
                     /locus_tag="VW077_001245"
     CDS             1009..1749
                     /locus_tag="VW077_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9455263.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1746..2657
                     /gene="pfkB"
                     /locus_tag="VW077_001246"
     CDS             1746..2657
                     /gene="pfkB"
                     /locus_tag="VW077_001246"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9455264.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSDGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2654..4606
                     /locus_tag="VW077_001247"
     CDS             2654..4606
                     /locus_tag="VW077_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9455265.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4770..6995
                     /locus_tag="VW077_001248"
     CDS             4770..6995
                     /locus_tag="VW077_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9455266.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7040..7468)
                     /locus_tag="VW077_001249"
     CDS             complement(7040..7468)
                     /locus_tag="VW077_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455267.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENKSNQ"
     gene            7606..8748
                     /locus_tag="VW077_001250"
     CDS             7606..8748
                     /locus_tag="VW077_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9455268.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHSLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8757..9971
                     /gene="thiI"
                     /locus_tag="VW077_001251"
     CDS             8757..9971
                     /gene="thiI"
                     /locus_tag="VW077_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9455269.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            10106..10930
                     /locus_tag="VW077_001252"
     CDS             10106..10930
                     /locus_tag="VW077_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9455270.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            10943..12109
                     /locus_tag="VW077_001253"
     CDS             10943..12109
                     /locus_tag="VW077_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9455271.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            12617..14110
                     /locus_tag="VW077_001254"
     CDS             12617..14110
                     /locus_tag="VW077_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9455272.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            14123..>14930
                     /locus_tag="VW077_001255"
     CDS             14123..>14930
                     /locus_tag="VW077_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9455273.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRFNEMFGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIY
                     GSGGIMGYAKDWIVKKNSVIIGRKGNINKPILVRENFWNVDTAFGLEPVLE"
BASE COUNT         4503 a         2742 c         3312 g         4373 t
ORIGIN      
        1 tcatctgaaa gtattttaga ataattctct tcgtgaattt cttcaaaaca gatagcttca
       61 tcttaggtat gtgttaattg gatttcgtaa gctctattta caatcgtaaa gttatgcact
      121 gaaaaagttg tagttagtct tccagtttgc taaatttctc gaatagtatc ttttttaggt
      181 atgtgatttc tttttgcatt tttgagttag ataaggtata atggttttat tgtcttttgg
      241 ggtcgttacg gattcgacag gcattatgag gcatattttg cgactcgtgt ggcgacgtaa
      301 acgctcagtt aaatataact gcaaaaaata acacttctta cgctctagct gcctaaaaac
      361 cagcaggcgt gacccgattt ggattgctcg tgttcaatga caggtcttat tattagcgag
      421 atacgattaa gccttgtcta gcggtttgat aagagattga tagactcgca gtttctagac
      481 ttgagttatg tgtcgagggg ctgttaaaat aatacataac ctatggttgt agacaaatat
      541 gttggcaggt gtttggacgt gggttcgact cccaccggct ccattattcc tttgcattcc
      601 ttggtaaaac gttgttaaat caacgttttt tatttttatc tttggtattc ctttgcattc
      661 ttttgctaaa aagggagtca caaacagacc ctattttaaa aaaaggatag aaaaaaggat
      721 acaacatttg tcgcatccta aaaataatct tttttcgacg gaagacatgg gattcgaacc
      781 cacgcacgct attacacgcc taccgcgttt ccaacacggc ctcttaagcc tcttgagtaa
      841 tcttccaata cttactcaaa tagtctacca taaaggctct tatcttgcaa taaaaattct
      901 agaaataaga aaaaatgata gattttgaaa gaaaatgata aaaaatgctt gacttcgaaa
      961 gaaagtatgg tagaatgaat agtgtaaacg ataacaggag gtgattcagt gttaaaaaca
     1021 gaacgtaaac aactgatttt agaggagtta aatcaacatc atgtagtttc tctagaaaaa
     1081 ttagttagtt tgctagaaac gtcagaatca acggttcgaa gagacttgga tgagttggaa
     1141 gcggaaaaca agcttcgtcg tgtgcatggt ggagcagaac tcccccactc cttacaggaa
     1201 gaagaaacca ttcaagaaaa atctgtcaaa aaccttcaag aaaagaagtt gctggctcag
     1261 aaagcagcct ctctcattaa agaaaaagat gtcatcttta tcgatgctgg aacaacaact
     1321 gcttttttga ttcatgaatt ggtcaataag aatgttacag ttgtgaccaa ctccattcac
     1381 catgccgctc agttggttga aaagcagatt ccaactgtca tggttggagg aaacgtcaag
     1441 acggcgacag atgctagtat cggtggcgtt gctcttaacc agattaacca attgcacttt
     1501 gaccgtgcct ttatcggaat gaatggtgtt gacgatggct attatacgac tcctgatatg
     1561 gaggagggag ctgtgaaaag agctattttg gagaatgcca agcagaccta cgtcttggtg
     1621 gattcgtcaa aaattggaca aacttgcttt gccaaggtag caccactcaa acgcgctatc
     1681 gttatcacta gtcaagggca tgagctcttg caggttatta aggagaaaac ggaggtaata
     1741 gaagtatgat ttatacagtc acactcaatc catccattga ctatatcgtt cgtttggacc
     1801 aagtcaaagt tggtagtgta aatcgtatgg acagtgatga taagtttgct ggtgggaaag
     1861 gaatcaatgt cagccgtgtc ttgaaacgtt tgaatatacc aaatacagcg acgggattta
     1921 tcggtggctt tactggtaaa tttatcacag atactttagc agaggaagaa atcgagacac
     1981 gttttgtcca ggtggcagaa gatactcgta tcaatgttaa aatcaaagca gaccaagaaa
     2041 cagaaatcaa cggaacgggt ccaactgttg aatcggttca gctagaagaa ttgaaagcta
     2101 ttttatctag tctgacagca gaagatacag ttgtctttgc aggttcaagt gctaaaaatc
     2161 taggcaatgt tatctataag gatttgattt ccttgacgcg ccagactggt gcgcaagtgg
     2221 tctgtgactt tgaaggacag accttaattg atagtttgga ctaccagcct cttcttgtaa
     2281 aaccaaacaa tcatgaactt ggagcgattt ttggggttaa actcgaaagt ttagatgaaa
     2341 ttgagaaata cgctcgtgag ttactggcta agggtgctca aaatgttatt atctctatgg
     2401 ctggtgatgg tgcccttctt gtcacatctg atggagctta cttcgctaaa ccaatcaagg
     2461 gaacagtcaa aaattcagtt ggagctggtg attctatggt tgctggattc acaggtgaat
     2521 ttgtcaaatc aaaagacgca gtagaagcct tcaaatgggg agtggcttgc ggaacggcaa
     2581 ctaccttctc agatgacttg gcaacggcgg aatttattaa agaaacatat ggaaaagttg
     2641 aggtagaaaa acgatgaaaa ttcaagacct attgagaaaa gatgtcatgt tgctagattt
     2701 gcaggcaact gaaaaaacag ctgtcatcga cgagatgatt aaaaatttga cagaccacgg
     2761 ttatgtaaca gattttgaaa catttaaaga aggaattttg gcgcgtgaag ctttgacttc
     2821 tactggtttg ggtgatggaa tcgcaatgcc tcacagcaaa aacgctgctg tcaaagaagc
     2881 gacagttcta tttgctaagt caaataaggg tgttgactac gagagcttgg atggacaagc
     2941 aactgacctc ttcttcatga ttgcagctcc agaaggtgcc aatgatactc acttggcagc
     3001 cttggcagaa ttgtctcaat acttgatgaa agacggtttt gcagacaaac ttcgtcaagc
     3061 aacatctgca gaccaagtta tcgaactttt tgaccaagct tcagaaaaaa ctgaggaact
     3121 tgttcaagca cctgctaatg actctggtga ctttatcgta gctgttacag cttgtacaac
     3181 aggtattgcc cacacttaca tggcccaaga agcccttcaa aaagtagctg ctgaaatggg
     3241 ggttggtatc aaggtcgaaa ccaacggtgc tagcggtgtt ggaaatcaac taactgcaga
     3301 agatatccgt aaggctaaag ctattatcat tgcagcagac aaggccgttg aaatggatcg
     3361 atttgatgga aaaccattga tcaatcgtcc agttgctgac ggtatccgta agacagaaga
     3421 gctaattaac ttggctcttt caggagatac tgaagtctac cgtgccgcta atggtgccaa
     3481 agctgcaaca gcctctaacg aaaaacaaag ccttggtggt gccttgtaca aacacttgat
     3541 gagtggtgta tctcaaatgt taccattcgt tatcggtggt ggtatcatga ttgcccttgc
     3601 cttcttgatt gacggtgctt tgggtgttcc aaatgaaaac cttggcaatc ttggttctta
     3661 ccatgagtta gcttctatgt tcatgaaaat tggtggagct gcctttggtt tgatgcttcc
     3721 agtctttgcg ggttatgttg cctactctat tgctgaaaaa ccgggtttgg tagcaggttt
     3781 cgtggctggt gctattgcca aagaaggttt tgcctttggt aaaattcctt atgccgcagg
     3841 tggtgaagca acttcaactc ttgcaggtgt ctcatctggt ttcctaggtg cccttgttgg
     3901 tggatttatc gcaggtgcct tggttcttgc catcaagaaa tacgttaaag ttcctcgttc
     3961 actcgaaggt gctaaatcaa tccttctatt gccacttctt ggaacaatct tgacaggatt
     4021 tgttatgcta gctgtgaata tcccaatggc tgcaatcaac actgctatga atgacttcct
     4081 aggcggtctt ggaggaggtt cagctgtcct tcttggtatc gtccttggtg gaatgatggc
     4141 tgttgacatg ggtggaccag ttaataaagc agcttatgtc tttggtacag gtacgcttgc
     4201 agcaactgtt tcttcaggtg gttctgtagc catggcagca gttatggctg gaggaatggt
     4261 gccaccactt gcaatctttg tcgcaactct tcttttcaaa gataaattta ctaaggaaga
     4321 acgtaactct ggtttgacaa acatcatcat gggcttgtca tttatcactg agggagcgat
     4381 tccatttggt gccgctgacc cagctcgtgc gattccaagc ttcatccttg gttcagcagt
     4441 agcaggtgga ctcgttggtc ttactggtat caaactcatg gcgccacacg gaggaatctt
     4501 cgttatcgcc cttacttcaa atgctctcct ttacctcgtt tctgtcttgg taggagcaat
     4561 cgtaagtggt gtggtttatg gttacctacg caaaccacaa gcataaaaaa tagaaaaatg
     4621 aaaagattgg accgtttggt gcagtctttt tctcttcccg aaatgcctgt gaaatatggt
     4681 ataatagaag aatggcaaac aagaatacaa gtacaacaag acggagaccg tctaaagcag
     4741 aactggaaag aaaagaagcg attcaacgaa tgttgatttc gttaggaatt gcgattttat
     4801 tgattttcgc agccttcaaa ttaggggctg caggtataac cctttataat ttaattcgct
     4861 tgctagtggg tagcctagct tatctggcga tattcggcct attaatctat ctcttctttt
     4921 tcaagtggat acgaaaacag gaaggactct tatctggctt tttcaccata tttgctggct
     4981 tactcttgat ttttgaggcc tacttggttt ggaaatatgg tttggacaag tccgttctaa
     5041 aagggaccat ggctcaggtt gtgacagatc tgactggttt tcgaacgact agctttgctg
     5101 gagggggctt gatcggggtc gctctttata ttccaacagc ctttctcttt tcaaatatcg
     5161 gaacttactt tattggttct atcttgattt tagtgggttc tctcctagtc agcccttggt
     5221 ctgtttacga tattgctgaa tttttcagta gaggctttgc caaatggtgg gaagggcacg
     5281 agcgtcgaaa agaggaacgc tttgtcaaac aagaagaaaa agctcgccaa aaggctgaga
     5341 aagaggctag attagaacaa gaagagactg aaaaagcctt actcgatttg cctcctgttg
     5401 atatggaaac gggtgaaatt ctgacagagg aagctgttca aaatcttcca cctattccag
     5461 aagaaaagtg ggtggaacca gaaatcatcc tgcctcaagc tgaacttaaa ttccctgaac
     5521 aggaagatga ctcagatgac gaagatgttc aggtcgattt ttcagccaaa gaagcccttg
     5581 aatacaaact tccaagctta caactctttg caccagataa accaaaagat cagtctaaag
     5641 agaagaaaat tgtcagagaa aatatcaaaa tcttagaagc aacctttgct agctttggta
     5701 ttaaggtaac agttgaacgg gccgaaattg ggccatcagt gaccaagtat gaagtcaagc
     5761 cggctgttgg tgtaagggtc aaccgcattt ccaatctatc agatgacctc gctctagcct
     5821 tggctgccaa agatgtccgg attgaagcac caatccctgg gaaatcccta atcggaattg
     5881 aagtgcccaa ctccgatatt gccactgtat ctttccgaga actatgggaa caatcgcaaa
     5941 cgaaagcaga aaatttcttg gaaattcctt tagggaaggc tgttaatgga accgcaagag
     6001 cttttgacct ttctaaaatg ccccacttgc tagttgcagg ttcaacgggt tcagggaagt
     6061 cagtagcagt taacggcatt attgctagca ttctcatgaa ggcgagacca gatcaagtta
     6121 aatttatgat ggtcgatccc aagatggttg agttatctgt ttacaatgat attccccacc
     6181 tcttgattcc agtcgtgacc aatccacgca aagccagcaa ggctctgcaa aaggttgtgg
     6241 atgaaatgga aaaccgttat gaactctttg ccaaggtggg agttcggaat attgcaggtt
     6301 ttaatgccaa ggtagaagag ttcaattccc agtctgagta caagcaaatt ccgctaccat
     6361 tcattgtcgt gattgtggat gagttggctg acctcatgat ggtggccagc aaggaagtgg
     6421 aagatgctat catccgtctt gggcagaagg cgcgtgctgc aggtatccac atgattcttg
     6481 caactcagcg tccatctgtt gatgtcatct ctggtttgat taaggccaat gttccatctc
     6541 gtgtagcatt tgcggtttca tcaggaacag actcccgtac gattttggat gaaaatggag
     6601 cagaaaaact tcttggtcga ggagacatgc tctttaaacc gattgatgaa aatcatccag
     6661 ttcgtctcca aggctccttt atctcggatg acgatgttga gcgcattgtg aacttcatca
     6721 aggctcaggc agatgcagac tacgatgaga gttttgatcc aggtgaggtt tctgaaaatg
     6781 aaggagaatt ttcggatgga gatgctggtg gtgatccgct ttttgaagaa gctaagtctt
     6841 tggttatcga aacacagaaa gccagtgcat ccatgattca acgtcgtttg tcagttggat
     6901 ttaaccgtgc gacccgtctc atggaagagc ttgagatggc aggtgtcatt ggtccagctg
     6961 aaggtaccaa accaagaaaa gttttacaac aataaaaaaa tagcttcttt ccaagtttgg
     7021 aaggaggcta ttttagtggt tattgattac ttttattttc tgaagttgga gtattgggct
     7081 gtttttcatt ttcagtggca ggtttacttg tagcaggtgt agtagagtcc tgagttgctg
     7141 ttttctgatc ttcttttttc tcttccttga cgctagattt tggtgtttcc tcttgctgtg
     7201 ttttttcttg actagtgtta gtctctttag ttggactggt gttttcctta ggggattcct
     7261 tttggatttc tttgacaatg gttgtcgtct ggcttgtcgt aggttctttt ttaatatttt
     7321 tgttattatc caaggcgttc atgatcgtga tactcgcggt aatcaagcca aggatactag
     7381 cgattgtagc aacggttttt tgaaagactg taaagccttt tttgatgtgt tctgtttttg
     7441 gttttgaagt tctacgatag ttagacatac attctctcct ttgattatga tttattatac
     7501 tctgtttctt taaaaaaatc ttaaaaaaag agaaattgcc ctttcttgtc gcaagctccg
     7561 tttcatgata caatagagga agtgatttta gaaagcgtga gaaacatgat ttactttgat
     7621 aattcagcga cgaccaagcc ttatcctgaa gcccttgaaa cctatatgca ggtcgcttca
     7681 aaaattttag gaaacccatc tagtctccat agtttgggag accaagcaac acgaatctta
     7741 gatgcttctc gccaacagat tgcagattta atcggtaaga aaagcgatga aatcttcttt
     7801 acctctggtg gaacagaagg ggataactgg gttatcaagg gtgtggcctt tgaaaaagct
     7861 cagtttggca agcacatcat tgtttcagcc attgaacatc cagcagtcaa agagtcagcc
     7921 ctctggttga aaagtcaagg atttgaagtg gattttgctc cagttgataa caaaggattt
     7981 gtggatgttg aggcgctaga agatttgata agacctgata cgaccctcgt ttccatcatg
     8041 gctgtgaaca atgaaatcgg ctctatccaa cctattgagg ctatttcaga attcttggca
     8101 gacaagccaa ctatttcctt ccacgttgat gcggttcagg cgcttgccaa gattccgact
     8161 gaaaagtatc tgacagaacg agtggatttc gcgactttct ctggtcacaa gttccacgga
     8221 atccgtggtg ttggttttat ctatatcaag tctggcaaga agattacgcc tcttttaaca
     8281 ggtggtggtc aggaacgtga ttaccgttcg acaactgaaa atgtggcagg aattgcagcg
     8341 accgctaagg ctctccgttt atctatggaa aagctagata tctttaggag caagactggg
     8401 cagatgaagg cagtgattcg tcaagcactt ctggactatc cggatatatt tgtcttttca
     8461 gatgaggaag acttcgcccc tcatattctg acttttggaa tcaagggtgt tcgtggtgag
     8521 gtcattgttc acgcctttga agactatgat attttcatct caacgacctc ggcttgttcg
     8581 tccaaggctg ggaaaccagc cggaaccctg attgctatgg gagtggacaa ggataaggct
     8641 cagtcagctg tgcgtcttag tctagacctt gaaaatgata tgagtcaggt cgagcagttc
     8701 ttgaccaagc taaaattaat ttacaatcaa actagaaaag taagatagga gcattcatgc
     8761 agtattcaga aattatgatt cgctacggtg agctgtcaac caagggcaaa aatcgtatgc
     8821 gtttcatcaa taaacttcgc aataatattt cagacgtttt gtctatctat ccccaagtta
     8881 aggtaacagc agatcgcgac cgtgcccacg cttacctcaa tggagcagat tacacagcag
     8941 tagcagaatc gctcaaacaa gtatttggaa ttcaaaactt ttcccctgtt tataaggttg
     9001 aaaaatctgt agaagttctg aagtctgctg tccaagagat tatgcaggac atctacaagg
     9061 aaggcatgac ctttaaaatc tctagcaagc gtagcgacca cacctttgaa ttggacagtc
     9121 gtgaactcaa ccaaacgctt ggaggggctg tattcgaagc cattccaaac gtacaagctc
     9181 aaatgaaaag tcctgacatc aatcttcagg tggagatacg tgaagaagca gcctatcttt
     9241 cttatgaaac cgttcgaggg gcaggtggat taccagtggg aacttctggt aagggcatgc
     9301 tcatgttgtc agggggaatc gattcccctg tagctggcta tctagcgctt aaacgagggg
     9361 tagatataga ggcagttcac tttgccagcc caccttacac gagtcctggt gctcttaaaa
     9421 aagcccaaga tttgacccgt aaattaacca aatttggggg aaatatccag tttatcgaag
     9481 ttccttttac agagattcaa gaggaaatca aagccaaagc gccagaagcc taccttatga
     9541 ccttgacgcg tcgttttatg atgcgtatta ctgaccgtat tcgtgaggta cgaaatggtt
     9601 tagttatcat caatggggaa agtctaggtc aagtagccag ccaaaccctt gaaagtatga
     9661 aggctatcaa tgctgttacc aacactccca tcattcgtcc tgtggttacc atggacaagt
     9721 tggaaatcat tgacatcgcc caggaaatcg atacctttga catttcaatc caaccgtttg
     9781 aagactgttg taccattttt gcaccagatc gtccaaaaac aaatcctaaa attaagaatg
     9841 cggagcagta cgaagcgcgt atggatgttg aaggcttggt tgagcgagca gtggctggaa
     9901 tcatgattac tgaaatcaca cctcaagccg aaaaagatga agttgatgac ttgattgaca
     9961 atctgctcta attcagaaaa tccaaaagaa tagcgaaaat cagtaaaaaa agttagtttt
    10021 ttctctaaaa acaggtaaaa aactaacttt ttttattttt atgatataat gatataaaat
    10081 tttgaatata gagagttttc tgacaatgaa tcaatcctac ttttatctaa aaatgaaaga
    10141 acacaaactc aaggttcctt atacaggtaa ggagcgccgt gtacgtattc ttcttcctaa
    10201 agattatgag aaagatacag accgttccta tcctgttgta tactttcatg acgggcaaaa
    10261 tgtttttaat agcaaagagt ctttcattgg acattcatgg aagattatcc cagctatcaa
    10321 acgaaatccg gatatcagtc gcatgattgt cgttgctatt gacaatgatg gtatggggcg
    10381 gatgaatgag tatgcggctt ggaagttcca agaatctcct atcccagagc agcagtttgg
    10441 tggtaagggt gtggagtatg ctgagtttgt catggaggtg gtcaagcctt ttatcaagca
    10501 caaaacaggt tggtttgatg gcatgatgac gacaggttgc tctatgggag cctatcatgc
    10561 actcaatttc ttcctccagc atccagatgt ctttaccaaa gtgattgctc tcagtggtgt
    10621 ttacgacgca cgtttctttg tcggtgatta ctacaacgat gatgctattt accaaaactc
    10681 gccagtagat tatatttgga accaaaacga tggctggttt attgaccgtt accgtcaggc
    10741 agagattgtg ctgtgtacgg ggcttggagc ctgggaacaa gatggtttac catcctttta
    10801 caagctcaaa gaagcctttg accagaaaca aattccagcc tggtttgctg aatggggaca
    10861 tgatgtcgcc cacgactggg aatggtggcg taaacaaatg ccttatttcc tcggtaatct
    10921 ctatttataa aaggagttac ctatgaatta ccttgttatt tctccctact atccacaaaa
    10981 ctttcaacag tttaccatcg aactagctaa taaaggcatc acagtcttgg gaattggtca
    11041 agagtcttac gagcaattgg atgagccctt gcgcaatagc ttgaccgagt attttcgtgt
    11101 tgataatctt gagaacatag atgaagtcaa acgtgcagtt gcttttctct tttataaaca
    11161 tggtccaatt gaccgcatcg agtctcacaa tgaatactgg cttgagctag acgcaacact
    11221 cagagaacaa ttcaatgttt ttggtgccaa accagaggat ctcaaaaaga cgaaatataa
    11281 gtctgaaatg aagaaacttt tcaaaaaagc aggtgttcct gtggtacctg gagctgttat
    11341 caagacggaa gcagatgttg atcaagcagt gaaagaaatc ggtcttccaa tgattgccaa
    11401 acctgataat ggagtgggag cagccgcaac ctttaaactt gagacagaag acgatatcaa
    11461 tcacttcaag caagaatggg accattcaac cctttatttc tttgaaaaat ttgtcacttc
    11521 cagcgaaatc tgtacctttg acgggctcgt ggacaaggat ggaaagattg tcttctcaac
    11581 aacctttgac tacgcctata caccgcttga cctcatgatt tataagatgg acaattctta
    11641 ttatgtgctc aaggatatgg atcctaaact gcgcaagtat ggggaagcaa ttgtcaaaga
    11701 atttggtatg aaagaacggt ttttccatat tgagttcttc cgtgaggggg acgattatat
    11761 taccatcgag tacaataacc gccctgcagg tggttttacc attgatgttt ataactttgc
    11821 tcattccttg gacctttatc gtggctatgc agctattgtc gcaggagagg agttcccggc
    11881 gtcagacttt gaaactcagt attgtttggc tacttctcgc cgtgcaaatg ctcactatgt
    11941 ttattcagaa gaggatttgc ttgccaaata tagccagcag ttcaaggtta aaaaagtcat
    12001 gccagctgcc ttcgcggaac ttcaaggaga ttacctgtat atgctgacaa ctccgagtcg
    12061 acaagaaatg gatcagatga ttgctgattt cggacaacgt caagaataag aactatcgga
    12121 ttaaggaaat tcactccctt aatccttttg ttttgtctgg taaaaaataa gagcatccta
    12181 acaaggtagc tatcataaaa cttgttcgat aactatttga agcaggatta ggtggctaga
    12241 aattgaattt taacatttca atttagtcat agtattgtgt tttcgtatcc ttaaatcagc
    12301 taaaaggatc catgacgaca cctatacgat atagttttca agataccaaa caagtctatt
    12361 aatattcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggtttc tcaaaacact
    12421 gttttgaggt tgtggataga actgacagag tcagtatcat atacctacgg caaggtgaag
    12481 ctgacgtggt ttgaagagat tttcgaagag tataaaatat tcaggtgacg catagatata
    12541 gttaattgaa gctttgtttg aaatctgata aaataatgat attactaagt tttaaaaact
    12601 aaagaaaagg gaagatatga ttacaggcga attaaaaaat aaaatcgatc agctgtggga
    12661 aattctttgg acagaaggaa acgcaaatcc tttaacaaat attgaacagt tgacttatct
    12721 cttatttatg aaagatttgg atagtgtcga gcttggacgt gaaagtgatg ctgaatttct
    12781 agggattcct tatgagggag tttttccaaa agataaacct gaataccgtt ggtcaacttt
    12841 taaaaatata ggagatgctc aggaagttta tcgtttaatg actcaggaga tttttccgtt
    12901 tattaaaaat ctcaaggggg atacagatga tacagccttt tcacgatata tgcgagaagc
    12961 tatttttcaa ataaataaac ctgctacgct tcaaaaggca atttctatct tagatgtttt
    13021 tccaactagg ggattagatg tagattttga taatgacaaa caaagtatta ctgatatcgg
    13081 agatatctat gaatatctgt tatcaaaatt gtcgaccgca ggtaaaaatg gacagttccg
    13141 tacacctcgt cacatcatcg atatgatggt tgagttgatg caaccgacta tcaaagatat
    13201 catctcagat cccgctatgg gttctgctgg cttcttagta tctgctagcc gttacttaaa
    13261 gcgtaagaaa gatgaatggg aaaccaatac agataatatc aatcattttc ataatcagat
    13321 gtttcatgga aatgatacgg atacgactat gttgagactt ggggcgatga acatgatgct
    13381 acatggagta gaaaatccac aaatcagtta ccttgactcg ctgtctcaag ataatgaaga
    13441 agccgataaa tatactttgg ttttagcaaa tcctcctttt aagggctcac ttgactacaa
    13501 ttcaacctct aatgaccttc ttgcaaccgt aaaaaccaaa aaaacagaat tactctttct
    13561 ttctcttttc ttgcgaactt taaaaccagg tggacgagca gcagttatcg tacctgatgg
    13621 tgtccttttt ggttcgtcta aagctcataa aggaattcgt caggaaattg tagagaatca
    13681 taagcttgat gctgtaatct caatgcctag tggtgtgttc aagccttatg ctggagtttc
    13741 aactgccatt ctcatcttta caaaaactgg taatggtggt actgacaaag tctggtttta
    13801 cgatatgaaa gcggatggtt taagtttgga tgataagcga caaccgatta gcgacaatga
    13861 tattccagat attatcgaac gctttcatca tcttgaaaaa gaagcagaac gtcagagaac
    13921 ggatcaatct ttctttgttc cagttgctga gataaaggaa aatgattatg atttgtctat
    13981 caataaatat aaagagattg agtatgaaaa agttgagtat gaaccaacag aggtcatctt
    14041 aaagaaaatc aatgatttag aaaaagaaat tcaagctggc ttggctgaat tagaaaaatt
    14101 actcaagtag agaggtggct gtatgaaaaa agtgaagttg ggggaagtct tatctctaaa
    14161 aaaaggcaag aaagccactg tacttgctga acaaacaact ctaagccaac gttatattca
    14221 aatagatgat ttaagaaata ataataattt aaaattcact gaaagtttaa atatgactga
    14281 agcactccca gatgatattc tgatagcatg ggatggagct aatgcaggaa cagttggtta
    14341 tggattatcg ggagctgttg gtagtacaat tacggtctta aaaaagaatg agcgatacaa
    14401 agaaaaaatt atatcagatt acttgggagt ctttttggaa agtaaatcgc agtatttacg
    14461 agaacattca acaggtgcaa caattcctca tttaaacaag aatatattac ttgatttaca
    14521 attagaattg ctaggtatcg aagaacaaga gaacattatc tgtattctta atacgattaa
    14581 aaggcttatt actaaaagaa aattgcagtt agatgaacta aacttgctcg tcaaatcccg
    14641 atttaacgag atgtttgggg atgttattct aaatgaaaaa gaatggaaag ttagcaaatg
    14701 gaacgagatt ctaacaataa gaaatggtaa aaatcaaaaa caggttgaag atgctgatgg
    14761 gaaatttcct atttatggaa gtgggggaat tatgggatat gctaaagatt ggatagtgaa
    14821 gaaaaattca gttattattg gacgtaaagg aaatataaat aaacctattt tagttagaga
    14881 aaatttttgg aatgtagata cagcatttgg attagagcct gttttagaaa
//
LOCUS       DAVKGU010000044        14650 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKGU010000044 DAVKGU010000000
VERSION     DAVKGU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.044"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(109..858)
                     /locus_tag="VW077_001256"
     CDS             complement(109..858)
                     /locus_tag="VW077_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9455274.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTNKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            991..3327
                     /locus_tag="VW077_001257"
     CDS             991..3327
                     /locus_tag="VW077_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9455275.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFGVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFSRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            3429..4106
                     /locus_tag="VW077_001258"
     CDS             3429..4106
                     /locus_tag="VW077_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9455276.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGCLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQNHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4093..4890
                     /locus_tag="VW077_001259"
     CDS             4093..4890
                     /locus_tag="VW077_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9455277.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4907..6010
                     /locus_tag="VW077_001260"
     CDS             4907..6010
                     /locus_tag="VW077_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9455278.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYVASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            6017..6163
                     /locus_tag="VW077_001261"
     CDS             6017..6163
                     /locus_tag="VW077_001261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455279.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVYRTDEVSNHTHGKETLTWFELD
                     FRRV"
     gene            6266..7285
                     /gene="galE"
                     /locus_tag="VW077_001262"
     CDS             6266..7285
                     /gene="galE"
                     /locus_tag="VW077_001262"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9455280.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTVTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7287..8240
                     /locus_tag="VW077_001263"
     CDS             7287..8240
                     /locus_tag="VW077_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9455281.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(8302..8508)
                     /locus_tag="VW077_001264"
     CDS             complement(8302..8508)
                     /locus_tag="VW077_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9455282.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSRDVLEAVKNCPTRALIGNQEA"
     gene            8560..9036
                     /locus_tag="VW077_001265"
     CDS             8560..9036
                     /locus_tag="VW077_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9455283.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9045..9716
                     /locus_tag="VW077_001266"
     CDS             9045..9716
                     /locus_tag="VW077_001266"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9455284.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            9847..10185
                     /locus_tag="VW077_001267"
     CDS             9847..10185
                     /locus_tag="VW077_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9455285.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDIVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            10188..10817
                     /locus_tag="VW077_001268"
     CDS             10188..10817
                     /locus_tag="VW077_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9455286.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11057..12208
                     /locus_tag="VW077_001269"
     CDS             11057..12208
                     /locus_tag="VW077_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9455287.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKKASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            12521..13270
                     /gene="truA"
                     /locus_tag="VW077_001270"
     CDS             12521..13270
                     /gene="truA"
                     /locus_tag="VW077_001270"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9455288.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IALILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            13260..14033
                     /locus_tag="VW077_001271"
     CDS             13260..14033
                     /locus_tag="VW077_001271"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9455289.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LVKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14011..14472
                     /locus_tag="VW077_001272"
     CDS             14011..14472
                     /locus_tag="VW077_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9455290.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKFPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         4044 a         2839 c         3429 g         4338 t
ORIGIN      
        1 acagtcaaat cgatttctaa aaatgtttta gaagtagagg tgtactattc tagttccaat
       61 ctactatata aatgaaaacc ttagctgttt cgccaaggtt catttcttct atgcaagttc
      121 ttctcttttc ttatctgggt tccagatgaa gcttgcgata aagctaaaga tgatggttag
      181 gatagcgagg ataatagcaa ggatgagggc agggatgcgg ataaaccacc agagtggatt
      241 tggtttttta tcattgtgcc attgtttcgt ttcttcgtcc agacgttgga attctgtttg
      301 aatacgattg gcgactgttt caatgccttc atcattcatt ttcttgatat ctgagatatc
      361 gattggattt ccaaagttca tatcgacacg ttcacggcta atcaagccct tcaaagtcat
      421 gggaccggtg taggtaaccg gcatgatacg gaccttggcc attttggcaa tcagtgctgc
      481 gccccccttg acatcgtttg agtggcggct accacttgga aacatgatga gagagcggtc
      541 actttttttg agaacgttga taggatattt gatggctgag gcgctgggat tttcacggtc
      601 gatgggaaag gcgccacaca tacgaatcca ccaaccaaag atacggttgg taaagagttc
      661 tttttttgcc ataaagatga actgttttgg cttggtcgca aaggccatat aaacaggatc
      721 ccaccaggta cggtgaggcg caactaaaat ataattttca tcttgattag gaattttatt
      781 agtattatga tagtgagcat tgccattgat ggaccataag agcaatacaa ctaatccacg
      841 caaataagta taaaacatgc gatctccttc gattgttttc ttgttattat tataccttat
      901 caaaggaggg ctggcaaact tttcccttga ctagatgcat atttgggatg aaattagaat
      961 tctgttaaaa aaaatgatat aatagaattt atggataaaa ataagattat gggattaacc
     1021 caaagagaag tcaaggaaag acaggctgag ggtttggtca atgactttac cgcatcagcc
     1081 agtaccagca cttggcaaat cgttaaacga aatgtcttta ccctttttaa cgctttgaac
     1141 tttgccattg ctttggctct tgcctttgtg caggcttgga gcaatctggt cttctttgct
     1201 gttatctgct ttaacgcttt ttctgggatt gtgaccgagc tacgagccaa acacatggtg
     1261 gacaagctca atctcatgac caaggaaaag gtcaaaacca tccgtgatgg tcaggaagtt
     1321 gctcttaatc ctgaagaatt agtgctagga gatgtcattc gtttgtctgc aggagagcag
     1381 attcctagtg atgccttggt tttggaaggc tttgcggaag tcaatgaagc catgttaacg
     1441 ggagaaagtg atttggtgca aaaggaagtt gacggcttac ttttgtcagg aagtttccta
     1501 gccagtgggt cagttttatc tcaagttcac catgtcggtg cagacaacta tgctgccaaa
     1561 ctcatgcttg aggctaagac cgttaaaccc atcaactccc gtatcatgaa atcgctggac
     1621 aagttggctg gttttactgg gaagattatc attccctttg gtctggctct cttgctggaa
     1681 gccttgcttt taaaaggcct gcctctcaag tcatccgttg taaactcgtc gacagctctt
     1741 ttgggaatgt tgcctaaggg aattgccctt ttgaccatta cttcgctctt gactgcagtg
     1801 attaagttgg gcttgaaaaa ggtcttggtg caggagatgt actctgttga gaccttggcg
     1861 cgcgtggata tgctctgtct ggacaagacg ggcaccatca cccaaggaaa gatgcaggtg
     1921 gaggctgttc ttccactgac ggaaacttac ggtgaagagg ctattgccag catcttgact
     1981 agctacatgg cccatagtga ggataagaat ccaactgccc aagccattcg ccagcgtttt
     2041 gtgggagatg ttgcttatcc tatgatttcc aatcttccct tctcgagcga ccgcaagtgg
     2101 ggggctatgg agttagaagg cttggggaca gttttcttag gggcacctga gatgttgctg
     2161 gattctgaag tcccagaagc cagggaggcc ttggagagag gatcacgtgt cttggtctta
     2221 gctctcagtc aggagaaatt agaccatcac aaaccacaga aaccatctga tattcaggct
     2281 ctagccttgc tggaaatctt ggaccccatt cgagagggag cagcagagac gctggactat
     2341 ctccgttctc aggaggtggg actcaagatt atctctggtg acaatccagt tacggtgtcc
     2401 agcattgccc agaaggctgg ttttgcggac tatcacagct atgtagattg ctcaaaaatc
     2461 accgatgagg aattgatggc catggcggag gagacagcta ttttcggacg tgtttcccct
     2521 catcaaaaga aactcatcat ccaaacgttg aaaaaagcgg gtcatacaac ggctatgaca
     2581 ggggacgggg ttaatgatat cttggccctt cgtgaggcgg attgttctat cgtgatggcg
     2641 gagggggatc cagcaacccg tcagattgcc aatctggttc tcttgaactc agactttaat
     2701 gatgttcctg agattctctt cgagggtcgt cgcgtggtca ataacattgc ccacatcgcc
     2761 ccgattttct tgataaagac catctattcc ttcctgttag cagtcatctg tattgccagt
     2821 gctttactag gtcggtcaga gtggattttg attttcccct tcattccgat ccagattacc
     2881 atgattgacc agtttgtgga aggtttccca ccattcgttc tgacttttga gcgaaatatc
     2941 aaacctgtcg agccaaactt cctcagaaga tccatgcttc gtgccctacc aagtgccctc
     3001 atggtcgtgt ttggcgttct ttttgtgaaa atctttggaa cgagtcaagg ttggtctgag
     3061 ttagaaatct caactctcct ctattatctc ttggggtcaa ttggtttctt atccgtattt
     3121 agagcctgca tgccattcac tctatggcga gtcctattga ttgtctggtc agtaggaggc
     3181 ttcctagcca cagctctctt ctcaagaatt caaaaactgc ttgaaattgc aaccttaaca
     3241 ggacaaacat tacctgttta tggtgtcatg atgttggtct ttaccgtgat tttcatcctg
     3301 accagtcgtt accaagcgaa aaaataaaga aagactgcaa tctgtggatt gcagtttttt
     3361 taggtacaag attgccagcc gaaatgtggt ataataagag gtaatagagt tttggaaagt
     3421 gagagaagat gatttcaaag agattagaat tggtagcttc ctttgtgtca cagggggcta
     3481 ttttactaga tgtgggaagt gaccatgctt atctgcctat cgagttggtt gagagaggcc
     3541 aaatcaaaag cgctattgca ggtgaggtgg tggaaggtcc ctatcagtct gcggttaaaa
     3601 atgttgaggc tcacggccta aaggagaaaa tccaagtccg tttagccaat ggcttggcag
     3661 cttttgaaga gactgaccaa gtgtctgtca ttaccattgc tggcatgggt ggttgtttga
     3721 ttgctaggat tttagaagaa ggtttgggga agttagctaa tgtagagcgt ttgatcctcc
     3781 agcccaataa tcgtgaagac gacttgcgta tctggctaca gaatcatgga ttccagattg
     3841 tagcagaaag catcttagaa gaagctggca agttttacga gattttggtg gtggaagcag
     3901 gacaaatgaa gctatcagcc agtgatgttc gctttggtcc cttcttgtcc aaagaagtca
     3961 gtccagtatt tgtccaaaaa tggcaaaaag aagctgagaa gctagagttt gccctcggac
     4021 aaatcccaga aaaaaatctg gaggaacgtc aagttctagt agataagatt caagctatca
     4081 aggaggtgct ccatgttagc aagtgaagtg atccaagctt atgaagcctt ttgccctcag
     4141 gaattttcta tggagggaga cagccgtggt ctgcaaattg gcactttaga caagggtatc
     4201 caaagggtca tggttgccct cgatattcgt gaagagacgg tggcagaggc cattgaaaag
     4261 ggtgtggact tgattatcgt caagcacgcg cctatctttc gtcctatcaa ggacttgctt
     4321 gccagccgtc cgcaaaatca gatttacatc gacctgatta agcatgacat cgcagtttat
     4381 gtcagccata ccaatattga tatcgttgaa aatgggctca atgactggtt ttgtcagatg
     4441 ctaggaatcg aggagacgac ttatctgcag gaaacaggtc cagaacgtgg aattggacgt
     4501 attgggaata ttcagcctca gacattttgg gaattggccc aacaggtcaa gcaagtcttt
     4561 gacctagata gccttcgaat ggtgcattat caagagaatg atttgcagaa gcctatttca
     4621 agagtagcaa tttgtggtgg aagcgggcag tctttctata aggatgcttt ggcaaagggg
     4681 gcagatgtct atatcactgg tgatatctac taccatactg ctcaggatat gctgtctgat
     4741 ggcttgttag cattggatcc aggtcactat atcgaagtgc tttttgtgga aaaaatcgca
     4801 gcactcctta ctcaatggaa agagaaaaaa ggctgggaac tagagatttt acctagtcaa
     4861 gcatcgacca atcctttccg tcatatctag ttagaaagtg aagacaatga aaaaagttgc
     4921 cattattgga gcaggaattg tgggagcaac agctgcctac tacctctcga gagaaagtga
     4981 cctagaggtg accgtttttg accatgagca aggtcaagcc accaaggccg cagcaggaat
     5041 tatcagtcct tggttttcca aacgccgtaa taaagcctgg tacaagatgg cgcgcttggg
     5101 ggctgacttt tatgtggatt tattagctga tttagagaaa tcaggacaaa aaatcgactt
     5161 ttaccagcgt tcgggagtct ttctcttgaa aaaggatgaa tccaagttgg aagaacttta
     5221 tcaactggcc ctccagcgca gagaagaatc tcccttgata gggcaattag ccattctgga
     5281 ccaagcttca gctaatgaat tattccctgg tttgcaggga tttgaccgcc tgctctatgc
     5341 ttctggtgga gcgagagtag atggccaact cttggtaact cgtctgctag aagccagtca
     5401 tgtcaagctg gtcaaagaaa aagtgactct gacactctta tcatcaggct accagattgg
     5461 cgaagaggtg tttgatcagg ttattttggc aacgggagct tggttggggg acatgttaga
     5521 gcctttaggt tatgaagtgg atgtccgtcc tcaaaaagga caactacgag attatcagct
     5581 tgcccaagac atggaagatt accctgttgt catgccagaa ggggagtggg atttgattcc
     5641 ctttgcaggt gggaaattat ccttgggcgc tacccatgaa aatgacatgg gatttgattt
     5701 gacggtagat gaacccttgc tccaacaaat ggaggaggcg gccttgcctc attacccagt
     5761 tttagctaga gcgacttcaa gggctgagcg tgtgggaatc cgtgcctata ccagtgattt
     5821 ctctcccttc tttgggcagg tgccagaatt ggcaggtgtc tatgtagcca gtggactagg
     5881 ttcatcaggc ctcacaactg gtcctatcat tggttaccat ctagcccaac tgatccaaga
     5941 caaggagttg accttggacc ctctaaatta tccaattgaa aactatgtca aacgagtaaa
     6001 aagcgaataa tactcaatga aaatcaaaga acaaactagg aaactagccg cgggttactc
     6061 aaagcactgc tttgaggttg tgtatagaac tgacgaagtc agtaaccata cccatggcaa
     6121 ggagacgttg acgtggtttg aattggattt tcgaagagta taagcatttt actgaaattt
     6181 tatcaggtag tttaggatgg caaatgacat tccctgtcaa aaatggtaaa ataagaaaaa
     6241 ataatctgag aatcgaggaa aaaagatgca agaaaagatt ttggtaactg gtggtgccgg
     6301 ttttattgga acccacactg ttattgaatt gatccaagca ggccatcaag ttgttgtggt
     6361 ggataatctt gtcaatagca atcgtaagag tttagaagtt gttgaaagaa tcacaggagt
     6421 tgagattcct ttctatgagg cagatattcg tgacacagtt accctcagag atattttcaa
     6481 gcaggaagaa ccgactggtg tcattcactt tgctggtttg aaggctgttg gcgaatcaac
     6541 acgtatccct cttgcctact atgacaacaa tatcgctgga actgtcagcc ttttgaaagc
     6601 catggaagaa aacaactgta aaaacatcat cttcagttct tctgcgacag tttacgggga
     6661 tccgcacaca gtgcccatct tggaagattt cccactttca gtgaccaacc catacggtcg
     6721 taccaagctc atgctagagg aaattttgac cgatatttac aaagcagact cagaatggaa
     6781 tgttgtcttg cttcgttact ttaacccaat cggagcccat gagagtggtg atttgggaga
     6841 aaatccaaac ggtattccaa acaatctctt gccatatgtg actcaagtag ccgttggaaa
     6901 attagagcaa gtgcaagtgt ttggagacga ttacgatacg gaagatggaa caggtgttcg
     6961 tgactatatc cacgttgtcg atttggctaa gggtcacgtt gcagctttga aaaaaatcca
     7021 aaaaggttca ggactaaacg tttataacct tggaactggt aaaggttact cagttcttga
     7081 aattatccaa aacatggaaa aagcggtggg acgtcctatt ccttaccgca tcgtagaacg
     7141 tcgcccaggt gatatcgctg cctgctactc agacccagca aaagctaaag cagaactcgg
     7201 ttgggaagca gaactcgaca tcacccaaat gtgtgaagac gcatggcgtt ggcaaagcaa
     7261 gcatccaaat ggatttgaag actaagatga tgatttcaat catcgtccct tgtttaaacg
     7321 aagaggaagt acttcctctt ttttatcagg ctctggaagc tttacttcca gatttggaaa
     7381 cagaaatcga gtatgtcttt gtcgatgatg gatcaagtga tgggaccttg gaactcttaa
     7441 aggcctatcg ggagcaaaat ccggcagtcc attatatttc tttctctcga aattttggca
     7501 aagaagcagc cctttatgca ggcttgcaat atgcgacagg agatttggtg gtggtgatgg
     7561 atgcagacct ccaagatcct cctagtatgt tgtttgagat gaaaaatgta ctagacaaaa
     7621 atgtagactt ggactgcgtt gggacacgga gaactagtcg ggagggagaa cccttctttc
     7681 gcagtttctg tgctgttctc ttttatcgcc tcatgcaaaa aatcagccca gtagctctgc
     7741 cgtcgggtgt ccgtgacttt cgtatgatga gaaggtctgt ggtcgatgcc attttaagct
     7801 tgactgagtc caatcgtttt tctaagggac tctttgcctg ggtcggcttt aaaacccact
     7861 atctggacta tccaaatgtc gaaaggcagg ctggcaagac cagttggagt tttaggcaac
     7921 tcttttttta ctccattgaa gggattgtta atttttcaga tttccctttg actatagcct
     7981 ttgtagctgg tctcctatct tgttttcttt ctctgctgat gacctttttt gttgtggttc
     8041 ggaccctcat tttgggcaat ccgacatctg gttggacctc tctgatggct gttattctct
     8101 ttcttggagg cattcaactc ttgaccattg ggattctcgg taagtatatc agtaagattt
     8161 atttagagac taaaaaaaga ccactttatc ttatcaaaga aaaaagtgac cttcctgatt
     8221 ttacagaaaa aataaagtga aaagactata attttacatg gaaatgtgct aaactagagg
     8281 gagtggatta tcgccattga tttaggcttc ctggtttcca atcagggcgc gagttgggca
     8341 atttttaaca gcctctaaga catcccgact aggagaaatt tctttttcca gttggtcagg
     8401 gtcatcgtaa aaacgcacga ttccattatc gtggtaatca aataaatcag aataagtttg
     8461 gcaaagtcca caggcgatac atcgttcagg tataagtgtg attttcatgt tcatatttat
     8521 attgtaataa gaaaagtaaa aaaaaacaag gagtaaggta tggcaaaaga accgtggcaa
     8581 gaagatattt atgatcaaga agaatcaaga gcagggcgtc ggcatcgaaa ccacggaggg
     8641 gctgatagga tggctaatcg tattttgacg atcctagcta gtattttctt tgtaattgtg
     8701 gtggtgatgg tcatcgttct catctatcta tcatcggggg ggagtaatcg cacagcagcc
     8761 ttaaaagact ttcatgattc tgatgcaagt gtagtacaaa tctcatcttc aagtagttct
     8821 cagcctgagc agagttcaga gccagaatct acttctagta gttcagaaga agctgctaat
     8881 cctgaaggaa cgattaaagt tctcgcagga gaaggggaag cagctattgc cgctcgtgca
     8941 ggaatctcca ttgctcagtt agaggccttg aatcctgggc acatggctac aggatcttgg
     9001 tttgctaatc caggtgatgt tataaaaata aaataggagc cgatatgaaa acaattcaaa
     9061 ttgctattga tggtcctgct tccagcggta agagtacggt cgcaaagatt attgctaagg
     9121 attttggatt cacctacctt gatacaggag ctatgtatcg tgcagcgacc tatatggctc
     9181 ttaagaacca attaggagtt gaagaagtcg aagcccttct agccttgttg gaccagcatc
     9241 caatcagctt tggacgttca gaaactggag accagcttgt ttttgtagga gatgtggata
     9301 ttacccatcc tatccgtgaa aatgaagtga ccaatcatgt ttctgctatt gcagcaattc
     9361 ctgaagtgcg tgagaaactg gtttctctcc aacaagaaat tgcccagcaa ggcgggattg
     9421 tcatggatgg tcgcgatatt ggaactgttg tattgccaca agcagaattg aaaattttcc
     9481 tagtagcttc tgttgatgag agagcagagc gtcgttacaa ggaaaatatt gccaagggaa
     9541 ttgaaacaga ccttgaaacc ctaaaaaagg aaattgctgc gcgtgactac aaggatagtc
     9601 atcgtgagac ttctcctctc aaacaagcag aggatgctgt ctaccttgat acaactggtt
     9661 tgaacattca agaagtagtt gaaaaaatca aagcagaagc tgaaaaaaga atgtaaatgt
     9721 agaaaagccg atagattgat atcggttttt ttctaaattt gtcaaaatgc tgattttagg
     9781 gtataatagt tgctgttcga gaaattttga ttttcaaaga atagtgaatg atgataagga
     9841 gaaaccatga acaatttacc aaattgtcca aaatgtaact cagagtatgt ctacgaagac
     9901 ggtgccctac tggtttgccc agagtgtgct catgagtgga atcctgctga agttgcagaa
     9961 gtagaagaag gtcttgtcgc tatcgatgcc aacggaaata aattggctga tggtgatata
    10021 gtaactctca tcaaggactt gaaagtaaaa ggtgcgccaa aagatttgaa acaagggacg
    10081 cgcgtgaaaa atatccgcat cgtagaaggc gaccacaata tcgactgtaa gattgatggt
    10141 tttggtgcca tgaaacttaa atcagagttt gtgaggaaga tttaagcatg tcaaaacact
    10201 ataaacttgt attttatagc cgtatcttct tgtttctagc ggcttttacg ggagtttatc
    10261 ttgaaattac taggcatggt ggttttggga tgcttctcta ttacacggtt ctgtccaacc
    10321 tcttggtaac tatttttacc ctttatcttc taaaggttat gagccgtgta ggtgaaaatt
    10381 ggcaaaggcc aagtctcttg cgcttaaaag gtggggtcac catgagtatc atgattacct
    10441 gtgtgattta ccatttcctc ttggcgccca ttgcgactaa tttctatacc ctagaaaatt
    10501 tcctttgcca ctatatcgtt cccatctggt ttttagcgga taccctcttt tttgacaaac
    10561 agggtcaata caagatttgg gatccagcag tgtggaccat tttacccttt ctgtatatga
    10621 tgtttgctct ttttaatggc ttggttctaa aactcaatat tccaaatgcc aaggataatc
    10681 ctttccctta cttctttttg aatgtgaaca agggttggaa tgttgtgttt aagtggtgtc
    10741 tgattatctt tgttgcctat atggtagcag gatttatttt ctactttatc aagcaaatca
    10801 agagaaagtc atcctaatac tcttcgaaaa tctcttcaaa ccacgtcagt ttccatctgc
    10861 aacctcaaaa cactgttttg aacaacctgc ggctagcttc ctagtttgct ctttgatttt
    10921 cattgagtat aagatacaag ggattcattt tcgaatttaa gaaggagtct acaaaccaat
    10981 gaggcttctt cttttcttgc ttatttgata actatcaaaa aagtagaaga ttaagagaaa
    11041 tatagaaaga gatttcatga aacaattttt agaacgggcc agcattttgg ctctctccct
    11101 cgttttgatt acctcctttt ccatttcgag tgccctacca gccatgtttg actattatca
    11161 gggttattct aaggaacaaa ttgagctctt ggtgagcttg ccttcctttg gaatcatgat
    11221 gatgttactg ctaaatggtt tcttagaaaa aatatttcct gagcgcttac agattagttt
    11281 gggcttgctg attttatcat tgagcggtac agctcccttc tggtaccaag cctatccctt
    11341 tgtctttgga acacggcttc tctttggttt gggtcttggg atgatcaatg ccaaggccat
    11401 ttctattatc agtgaacgct atcaaggaaa aaggcgaatt cagatgttag ggctacgcgc
    11461 ttctgcagag gtcgttggag cttctctcat taccttggcc gtcggtcagt tgttggcctt
    11521 tggttggaca gctatctttc tagcctatag tgctggattt ttggtgctgc ccctttatct
    11581 gctctttgtc ccttatggaa aatcaaagaa agaagtcaag aaaagagcga agaaagcaag
    11641 tcgtttaact cgagaaatga aaggcttgat ttttacctta gctatcgaag cggcagttgt
    11701 agtttgtacc aatacagcta ttaccatccg tattccaagt ttgatggtgg aaagaggatt
    11761 gggggatgcc cagttatcta gttttgttct tagtatcatg cagttgatcg ggattgtggc
    11821 tggggtgagt ttttctttct tgatttctat ctttaaagag aaactgctcc tctggtctgg
    11881 tattaccttt ggcttggggc aaatcgtgat tgccttgtct tcatccttgt gggtggtagt
    11941 agcaggaagt gttctggctg gatttgccta tagtgtagtc ttgacgacgg tctttcaact
    12001 tgtctctgaa cgaattccag ctaaactcct caatcaagca acttcatttg ctgtattagg
    12061 ctgtagtttc ggagccttta cgaccccatt tgttctaggt gcaattggct tactaactca
    12121 caatgggatg ttggtcttta gtatcttagg aggttggttg attgtaatct ctatctttgt
    12181 catgtaccta cttcagaaga gagcttagga ttgattccta agtttttctt ttgatactca
    12241 atgaaaatca aagagcgtag ttgattgagt ttggaatagt acgctgtagc tgctaaaata
    12301 tttctagaaa ttaatttgac tttcctaata gagttgttca tatcttattt caatctacta
    12361 tagaaagcta gccgcaggtt gctcaaaata gtgttttgag gttgcagatg gaaactgacg
    12421 tagtttgaag agattttcga agagtatgag atttttatac ccatacaata tttgttttag
    12481 gtccttgttt acagactaat ttcttgataa aatagaggtt atgacgagat ataaagcaac
    12541 tatttcctat gatggttatg cctttgctgg ctttcagcgc cagcctcatg cgcgtagcgt
    12601 tcaggaagaa attgaaaaaa ccttgaccag attaaataaa gggcaaacca ttactgttca
    12661 cggtgctggt aggacagata gtggggttca tgccctggga caggtcattc attttgacct
    12721 gccttatcag atggatgagg agaaactccg ttttgccttg gatacccagt ctcctgaaga
    12781 tattgatgtg atttcgattg agcttgtggc agatgatttt cattgccgtt atgccaaaca
    12841 tagcaagacc tatgagttta ttgtggatag aggacgtccc aaaaatccta tgcgccgtca
    12901 ctatgccacc cactttccct acccactcga tgtggaacga atgcagattg caatcaaaaa
    12961 gctagaggga acccatgatt ttaccggttt tacagcctct gggactagtg tagaggataa
    13021 ggttcgcacc atcacagaag ctagtttaat agtcgatgag acaggacaat ttttgacctt
    13081 taccttttca ggaaatggtt tcttgtataa acagattcgc aatatggtgg ggactcttct
    13141 caaaatcggt aacaaccgca tgccagtaga gcagattgct ctcatcttgg agaagaagga
    13201 caggcaactt gcaggtccca ctgcagcacc aaatggtttg tatttaaagg agattcgtta
    13261 tgaagaataa tcgtatttta gcactttctg gaaatgatat ttttagtggt ggtggactgt
    13321 cagctgattt ggctacctat accttgaacg gcttgcatgg gtttgtagca gtgacttgtt
    13381 tgacagcctt gacagaaaaa ggatttgaag tctttccaac tgatgatacc atttttcaac
    13441 atgaattaga tagcttgcgt gatgtggaat ttgggggaat taagattggt cttctcccta
    13501 ctgtcagtgt ggctgagaag gccttggact ttatcaaaca acgcccagga gtacctgtgg
    13561 tgttggatcc tgtcttggtc tgcaaggaaa cgcatgatgt agctgtcagt gagctctgcc
    13621 aagagttgat tcgcttcttc ccttatgtca gtgtgattac gcctaatctc ccagaagcag
    13681 aattattatc cggtcaggaa attaaaacct tggaagacat gaaaactgca gcgcagaaat
    13741 tgcatgattt aggagcgcca gcagtcatta tcaagggagg caatcgtctt agtcaggaca
    13801 aggctgtgga tgtcttttat gatggacaga cctttactat cctagaaaat ccagttatcc
    13861 aaggccaaaa tgctggtgca ggttgtacct ttgcctctag cattgccagt cacctggtta
    13921 aaggtgataa acttttgcca gcagtagaaa gctctaaggc tttcgtttat cgtgctattg
    13981 cacaagcaga tcagtatgga gtaagacaat atgaagcaaa caaaaacaac taaaatcgcc
    14041 cttgtatccc tattaaccgc cctttctgtg gttctaggtt atttcttaaa attcccaaca
    14101 cctacaggaa ttctaactct tttagatgct ggtgtcttct ttgcggcctt ttactttggt
    14161 agtcgtgaag gagcggtagt cggaggacta gcaagtttct tgattgacct cttatcaggc
    14221 taccctcagt ggatgttctt tagcttggtc aaccatggct tgcagggatt tttcgcagga
    14281 tttaaaggaa aaagtcagtg gttaggcctt attttagcaa ctattgccat ggtaggaggc
    14341 tacgccttgg gttctacttt gatgaatggc tgggcagcag ccctcccaga aattctaccg
    14401 aattttatgc aaaatatggt agggatgatt gtaggattta ttcttagtca aagtatcaag
    14461 aagattaagt aatactcttc gaaaatctct tcaaaccacg tcagcttcca tctgcaacct
    14521 caaaacactg ttttgagcag cctgaggcta gctttctagt ttgcactttg attttcattg
    14581 agtataaaga ggttgagtaa aaagtctttc aaataagaaa aacgcattat atcaagtctt
    14641 gactgaactg
//
LOCUS       DAVKGU010000045        14130 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKGU010000045 DAVKGU010000000
VERSION     DAVKGU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.045"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            66..1232
                     /locus_tag="VW077_001273"
     CDS             66..1232
                     /locus_tag="VW077_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9455291.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            1222..2451
                     /locus_tag="VW077_001274"
     CDS             1222..2451
                     /locus_tag="VW077_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9455292.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELANLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLAAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            2597..3238
                     /locus_tag="VW077_001275"
     CDS             2597..3238
                     /locus_tag="VW077_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9455293.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            3248..3877
                     /locus_tag="VW077_001276"
     CDS             3248..3877
                     /locus_tag="VW077_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9455294.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            3890..4726
                     /locus_tag="VW077_001277"
     CDS             3890..4726
                     /locus_tag="VW077_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9455295.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(5004..5351)
                     /locus_tag="VW077_001278"
     CDS             complement(5004..5351)
                     /locus_tag="VW077_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9455296.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(5462..7180)
                     /locus_tag="VW077_001279"
     CDS             complement(5462..7180)
                     /locus_tag="VW077_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9455297.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            7414..7557
                     /locus_tag="VW077_001280"
     CDS             7414..7557
                     /locus_tag="VW077_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU9455298.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KNT"
     gene            7524..7739
                     /locus_tag="VW077_001281"
     CDS             7524..7739
                     /locus_tag="VW077_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9455299.1"
                     /translation="MGKERCEQEHIERKKRVVKNRKIPLEELKTFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            7726..8259
                     /locus_tag="VW077_001282"
     CDS             7726..8259
                     /locus_tag="VW077_001282"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9455300.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            8617..8871
                     /locus_tag="VW077_001283"
     CDS             8617..8871
                     /locus_tag="VW077_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455301.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            8982..10073
                     /locus_tag="VW077_001284"
     CDS             8982..10073
                     /locus_tag="VW077_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9455302.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEVDTNSASLTGNIVSKGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKSGSQNSNLY"
     gene            10045..10566
                     /locus_tag="VW077_001285"
     CDS             10045..10566
                     /locus_tag="VW077_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9455303.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKKDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDEK"
     gene            10789..11658
                     /locus_tag="VW077_001286"
     CDS             10789..11658
                     /locus_tag="VW077_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9455304.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            12580..13776
                     /gene="tuf"
                     /locus_tag="VW077_001287"
     CDS             12580..13776
                     /gene="tuf"
                     /locus_tag="VW077_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9455305.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT         4347 a         2517 c         3005 g         4261 t
ORIGIN      
        1 ctttaaaaaa gagttatttt gtttcaaaaa tggtataatg agagaacaat agaaaggaag
       61 tatttatgga gcaaaaagag aaacatttta gcctatcttg gtttttcaag tggtttttag
      121 ataacaaggc aattacggta tttttagtaa ccttattatt gggactgaat ctttttattt
      181 taagtaagat tagttttcta ttttcacctg ttttagactt tttggcagtt gtgatgttgc
      241 cagtcatttt gtctggtttg ttatattatt tgttgaatcc tattgttgat tggatggaga
      301 agcataaggt taatcgtgtt atagctatca ctattgtctt tgttatcatc gctctcttta
      361 tcatttgggg cttggcagtc gccattccaa atctgcaacg tcaggttttg acctttgcaa
      421 gaaacgttcc tgtttactta gaagatatag ataggattgt taatggattg gtagcccagc
      481 acctgccaga tgatttcaga cctcaattag agcaagtttt gaccaatttt tctagccagg
      541 ctacagtttt ggcaagtaag gtttcatctc aggcagtcaa ctgggtgagt gcctttatta
      601 gcggagcttc tcaagtgatt gttgccttga ttatcgttcc tttcatgctc ttttatctct
      661 tgcgtgatgg gaaaggcttg cgtaactatt tgacccaatt cattccaaga aaattgaagg
      721 aacctgttgg acaagttcta tcagatgtga atcaacagtt gtccaactat gttcgagggc
      781 aagtgacagt ggctattatt gtagcagtaa tgtttatcat cttcttcaag attattggtc
      841 tacgctatgc ggttacgctg ggggttactg ctggtatttt aaatctggtc ccttatcttg
      901 gtagctttct agccatgctt cctgctctag tattgggttt gattgctggt ccagtcatgc
      961 ttttgaaagt agtgattgtc tttatcgtag aacaaactat tgaaggccgt tttgtctctc
     1021 cattgatttt gggaagtcaa ttaaacatcc atcctattaa tgttctcttt gttttgttaa
     1081 cttcaggatc tatgtttggt atctggggag ttttacttgg tattccggtt tatgcctctg
     1141 ctaaggttgt catttcagcc attttcgaat ggtataaggt agtcagtggt ctatatgaat
     1201 tagagggtga ggaagtcaag agtgaacaat agtcaacaga tgttacaggc tttggaggag
     1261 caagatttaa ctaaggctga gcattatttc gccaaagctt tagaaaatga ttcaagtgat
     1321 cttctgtatg agttggcaac ttatcttgaa gggattggtt tctatcctca ggccaaggaa
     1381 atttacctga aaattgtaga ggattttcca gaggttcatc ttaatctagc tgcaattgct
     1441 agcgaggatg gtcaaataga agaagccttt acctatcttg aggaaatcca agctgacagt
     1501 gactggtatg tctcgtcttt ggctctgaag gcagaccttt accagctgga aggtttgaca
     1561 gatgtggcac gtgagaaatt attggaggcc ttgacctact cagaggattc tctcttgata
     1621 ttgggtttgg cagagttgga tagtgagttg gaaaattacc aagcggctat tcaagcctat
     1681 gcccagttag ataatcgctc gatttatgag caaacgggca tttccaccta tcaacgaatt
     1741 ggctttgcct atgctcagtt agggaaattt gaaacggcta ctgagttttt agaaaaagcc
     1801 ctggagttag aatacgatga cttaacagct tttgagttgg caaatcttta ttttgatcaa
     1861 gaagaatatc aaaaagccac cctctacttt aagcagcttg ataccatttc tcctgacttt
     1921 gaaggctatg agtatgggta cagtcaggct ttacataagg aacatcaagt tcaagaagcc
     1981 ctgcgtatcg ctaagcaagg attagagaaa aatccctttg aaactcgcct cttgctagct
     2041 gcttcacaat tttcttatga attgcatgat gctagtggtg cagaaaatta tcttcttgct
     2101 gcaaaagaag acgctgagga tacagaagaa atcttgcttc gtttagccac tatttatctg
     2161 gagcaggagc gttatgagga tattctagac ttgcagagtg aggagccaga aaatcttttg
     2221 accaagtgga tgattgctcg ttcttatcaa gaaatggacg atttggatac tgcttatgag
     2281 cattatcaag agttgacagg agatttgaag gacaatccag aatttctgga acactatatc
     2341 tatctcttgc gtgaattggg acattttgaa gaagcaaaag tccatgctca cacttactta
     2401 aaactggttc cagatgatgt gcaaatgcaa gaactgtttg agagattgta agaatgttta
     2461 aacatataga actgtagttt atctcttttg atagctacgg tttttatttg tatatggtag
     2521 aatcttttta caaaaatact tggtaatctt gtttattcat gctataatag gaacaattac
     2581 ttttaggagg tgcagtatgt cttatttatt tgagatatta ccgagtttac tgaatggtgc
     2641 gagcacgact gtacaggtct ttgcactggt cttgctattt tcgattccct tgggcgtttt
     2701 gattgccttt gccttgcaag tccattggaa gcccctccat tatctgatta acatttacat
     2761 ctgggttatg cgaggaaccc ccttactctt gcaactgatt tttatctatt atgtgctccc
     2821 aagtattggg attcgtttag accgccttcc tgcagctatt attgcctttg ttctcaacta
     2881 tgcagcttac tttgcagaaa ttttccgtgg gggaattgac actattccaa gaggacagta
     2941 tgaggccgcc aaggtcttga agtttagccc ttttgacaga gtgcgctata ttatcttgcc
     3001 ccaagtgacc aagatcgttc ttcctagtgt ctttaatgaa gttatgagtt tggtcaagga
     3061 tacttctttg gtctatgctc tcggaatttc agaccttatc ttggctagtc gaacagctgc
     3121 taaccgcgat gctagtctag ttcctatgtt cttggcagga gccatttatt tgattttgat
     3181 tgggattgtg acaattattt ccaaaaaagt tgagaagaag tatagttatt atagatagga
     3241 ggctgccatg ttagaattac gaaatatcaa taaagtcttt ggagacaaac aaatcctgtc
     3301 taatttcagt ctaagtattc ctgaaaagca aatcctggct atcgttggac cttctggtgg
     3361 aggtaagaca actcttttac gtatgcttgc aggtcttgaa accattgatt cagggcaaat
     3421 cttttataat ggacaacctt tagagctgga tgaattgcag aagcgcaatc tactgggatt
     3481 tgtattccaa gattttcaac tgttccctca tttatcagtt ctggaaaatt tgactttatc
     3541 gcctgtgaag accatgggaa tgaagcagga agaggctgag aagaaggcga gtggactctt
     3601 ggaacagtta ggactaggag gacacgcaga ggcctatcct ttctcactat ctggtgggca
     3661 aaagcagcgg gtggctttgg cgcgtgctat gatgattgac ccagaaatca ttggctacga
     3721 tgaaccaact tctgccctgg atccagaatt acgtttggaa gtggagaagc taatcttgca
     3781 aaatagggaa cttgggatga cccagattgt ggttacccat gatttgcagt ttgctgaaaa
     3841 tatcgcagat gtattattga aagtagaacc taaataggag gaaaaatgga tgaaaaaatg
     3901 gatgcttgta ttagtcagtc tgatgactgc tttgttctta gtagcttgtg ggaaaaattc
     3961 tagcgaaact agtggagata attggtcaaa gtaccagtct aacaagtcta ttactattgg
     4021 atttgatagt acttttgttc caatgggatt tgctcagaaa gatggttctt atgcaggatt
     4081 tgatattgat ttagctacag ctgtttttga aaaatacgga atcacggtaa attggcaacc
     4141 gattgattgg gatttgaaag aagctgaatt gacaaaagga acgattgatc tgatttggaa
     4201 tggctattcc gctacagacg aacgccgtga aaaggtggct ttcagtaact catatatgaa
     4261 gaatgagcag gtattggtta cgaagaaatc atctggtatc acgactgcaa aggatatgac
     4321 tggaaagaca ttaggagctc aagctggttc atctggttat gcggactttg aagcaaatcc
     4381 agaaattttg aagaatattg tcgctaataa ggaagcgaat caataccaaa cctttaatga
     4441 agccttgatt gatttgaaaa acgatcgaat tgatggtcta ttgattgacc gtgtctatgc
     4501 aaactattat ttagaagcag aaggtgtttt aaacgattat aatgtcttta cagttggact
     4561 agaaacagaa gcttttgcgg ttggagcccg taaggaagat acaaacttgg ttaagaagat
     4621 aaatgaagct ttttctagtc tttacaagga cggcaagttc caagaaatca gccaaaaatg
     4681 gtttggagaa gatgtagcaa ccaaagaagt aaaagaagga cagtaagata aaatagtggc
     4741 tgaaactgcg ttttgattag caaaacgtag tttttttgta atctaggaaa acgataatag
     4801 cgattgaatc tggataattg aatatggaat agcccactgt gatttctaaa acattgttaa
     4861 aaattgattt gacttccaaa attaaaatgt tctgtaatga aatgctgatg taactgtttt
     4921 aggaacaata aaacgcataa tatcaaggtt tttgcacctt acattatgcg tttttgtgat
     4981 tttaagactt gttagctgat tttttacaat cctgcgaaat ctttgatttc ttgtgctgac
     5041 attgaagagt cgcaacggac gttgatttgt ccatctgtaa tatgaacaaa acctggtaca
     5101 gttgggattc catagcgtga gcggaatgct tgcaaatcat tgagttggct tggttcttca
     5161 ctattgatga agtaaatgtg agctttggtt tcagctacga cacctgacaa tgtacctgca
     5221 aatttacggc agtaagggca agttttgcga ccgataaaga aggttgcagt ttctttttta
     5281 tcaagagctt cttgcgcacg cacaactgta gtgacttcaa ggtctttgat gttatctaaa
     5341 aattgttcca tgagattacc tcgctttcat tgataagtct agtatgccat aaagtttcta
     5401 aaattgctta gatttgatac gaaaaaagat gaggttggtt ggtctcatct tttataggtc
     5461 tttattttac aaatgcattg atttctgctt cgatgttagt aatcttagct tgtgattctt
     5521 cgttggtttc ccctacaact gcaatgtaga acttgatttt tggttctgta cctgaagggc
     5581 gaacggcaat ccatgaaccg tcagcaagtg tgtatttcaa cacatcactt ggaggagttg
     5641 tcaagtttgt aacagtaccg tcagcaacag tagcagtttg tgccttgaag tcttctacga
     5701 cagtgatagc tgttgcgttc cattctgttg gagcattgtt gcggaattta gccataatcg
     5761 ctttgatttg ttcagcacca tcgacacctg aaagagtaac agagattgtt ttttctgcgt
     5821 agtagccata ttctttatag atttcttcga taccgtcagc aagtgtcaaa ccacgagaac
     5881 ggtagtaggc agcaagttca gcaacgacaa gaacggcttg gatggcatct ttatcacgta
     5941 caaatggttt aatcaagtaa ccgaagcttt cttcaaatcc catcatgtaa gtgtggttgt
     6001 gtttttcttc gaattcttgg attttttcag cgataaattt gaaacctgtc aagacgttga
     6061 acatagttgc gccgtagctt tcagcaatct tcgttaccaa gtcagttgaa acgatagatt
     6121 tgcagagagc ggcattttca ggaagagttc cagcgttttt gtgagcttcc aagatgtatt
     6181 tagccatgat agcaccgatt tggttacctg aaaggttgag gtagctacca tctttttgaa
     6241 gaacttcaac accaacacgg tcagcgtctg ggtcagttgc gacaagaaca tctgcaccaa
     6301 cttgacgacc aagttcttca gcaagggcaa aggctgcttg gctttctggg tttggagatg
     6361 ttacagttga gaagtctggg tcagcagttg cttgcgcttc aacaacttga acagagtcaa
     6421 atcctgcttg ggcaagagca cgacgagcca acatttcacc agtaccatga agtggtgtgt
     6481 agacaatctt catgtcttta ccaaattctt caatcaaggc tgggttgatg tttatgtcct
     6541 taacctcttt aaggtattct atgtcaacag cttcgccgat aacttcaatc aagccagaag
     6601 ctttttcagt ttccacatca gcaacttcaa ctgcaaatgg gttttcgatt gcacggatat
     6661 aagtagtcaa agcgtccgca tcgtgtggag gcatttgtcc accgtcttca ccgtaaacct
     6721 tgtaaccgtt aaatggagca gggttgtggc tggctgtgac catgatacct gcgaaacagt
     6781 tgagatgacg aactgcaaat gatagttctg gagtcggacg aaggctttca aatacgtaag
     6841 atttgatgcc gtgtttagca agaactgccg cagattcaaa ggcaaactca ggtgagaagt
     6901 gacggctatc gtaggcaatt gctacaccgc gttctttctc gtttccacct tttgactcaa
     6961 tcaaacgagc caatccttca gtagcttggc gaacaacgta gatgttgata cggtttgtac
     7021 cagcaccaac caagccacgc atacctgcag taccaaattc aagatttgta tagaaggcat
     7081 cttccttagt tttttcgtcc atattttcca aatcttgacg aaggtagtca ggaagctcca
     7141 caaaatcaac ccatttctgg taattttctt ggtaagacat tcaaattctc ctttattttt
     7201 aaaacattta atcagtttga ttatatcatt tttttaagtt ttagtaaaac cttatctgct
     7261 tcgaacatct cttcaaacca ggtcagattg aattttgggg ttatatgatg ttgaggctag
     7321 gaaaaattca atttcagtaa aaaaagtaag tcttctcata acaaaacatt gatatagttg
     7381 cttagtttta aacaagcata ttataataaa gctatggcat atagtactga ttttaaacag
     7441 cgagcattag attacatcaa agaggggcac agccatgtcg aggcagccaa gttttttggt
     7501 gttggcgtca gaactctctt cacgtgggaa aagaaagatg tgaacaagaa cacatagaga
     7561 ggaaaaagcg agtcgtcaaa aaccgaaaga ttcctttaga ggaattgaaa acctttgtag
     7621 aggctcatcc agatgctttt ttacgggaaa ttgcggcaca ttttgattgt gctgttcctt
     7681 cagtatgggc agctttaaag cagattaagg tcactttaaa aaaagatgac gagctttaag
     7741 gaacaagagc cagagaaagt cgctgagttt cttgatattt tggataacct aaaagattta
     7801 ccaatcgtat atattgacga aacaggaatc gaccgctacc tctatcgtcc ttatgcaggg
     7861 gctcctagag gggagaaagt ctatgaaaag attagcggac gtcgttttga gcgaacttca
     7921 attgttgcag gacaagtaga cggagagttt atagctccca tgatttacaa gaaaagcatg
     7981 acaagcgatt tctttgtgga gtggttcaaa acgcaactcc tacctgcttt gaagacacct
     8041 catgttattg tcatgggcaa tgctagtttt catcccaaga acattttgga tgaactctgc
     8101 atccaagata aacacttttt cttacctcta ccaccttatt caccggattt gaatcctatt
     8161 gagcaagctt gggctatctt gaaaaagaaa gtgacggatt tattaaggga agttccaact
     8221 atttttgaat gtttggaatg cttttttaaa actagatgac tataacggtt ctaaaggaac
     8281 ctatcgagta gtcattaaaa ctaaggatac tgctggttaa gagaagacgg tatacaatca
     8341 aaccattcac cgtgtagccg aaatcgttca gaatgaagac ttgtatcaga atgaagactt
     8401 gtataagaaa ggtttgaatg ttgaacttgc gcaccaacaa attaagggat tttttgaagc
     8461 agaatttaaa aatcgtatta atggagttct taatactaaa ataaaaaata gtacattaaa
     8521 tcgtgtaaat aaaaaaacta tacaccagag caacaaaaac tccatgatca atttgaagca
     8581 gaagcaacgg aagatgctaa aaaacaaggc gatattgtgt tgaatgttga ccaggatttc
     8641 atgagcatat ctaagtctaa taaaagtggt tcagactgga agaaaacttt cacagtgagg
     8701 ataaccaata ggctagcaaa tgacttgaat aatgtcttga aacaggctga taaagatact
     8761 cctaataccc caacttggct aaactcagct gcttctaaag ctaaagatga tgacagagca
     8821 tataaactac tgaagactct tataccagga gaaaattacc tatcatgtta aggataatca
     8881 gctagaagta gaaacagata aatacacata tactgccgct agaaatggta gtaaggaagt
     8941 tggtattcaa gagtcagata tagcagcaac tctaagcgcc gatgaatata attctaatcg
     9001 ccaaaccttt gagagagaat acaaatacaa aagcaaatgc ccttaataat ggttgggcta
     9061 gatctggttc tgaagagttc aaaaaattct cccactttgt aggggtagac aaagggattg
     9121 tgcgaacgaa tgtactgact ggtaaaaaac tatctgataa gattaggaaa gaagtgggct
     9181 ctggagatag caaactagga aaaggcggct atttctctac tggggatgtt ctattaggaa
     9241 aagatgttgt ttcttatacc gtacaagtat tttcagagaa taatgaaaga gtaggagtaa
     9301 acactcaaag tcaccgtgtt cagtataatc tcccaattct agctgacttt tcagtcatcc
     9361 aagatactgt ggaaccatca cgaaccgttg ttgaaaaaat cattccaaaa ctaaatattc
     9421 ccgaagaaga gaaagggaaa ataaccgaag aaatcaagaa aaagaaaaaa acctcagaat
     9481 tggcagaact aatctcagaa aatgtgaaag ttcgctatgt tgatgaacaa gggcgtttgc
     9541 tatcattgaa aaatgatact ggaattggag aaaaagaaag tgacggaacc tacattacca
     9601 ataaaaaaca actgattggt accagctata atgtcacaga taaaaaactc agtagcatga
     9661 ctactactga cggaaaatat tatactttta aagaagtaga tacaaattct gcaagtttaa
     9721 ctgggaatat tgtaagcaaa ggtagaacag tgaccttagt ttatagagaa agcgaagcgc
     9781 caaccactgc tacagtaaca gccaattact ataaagaagg tagccaagag aagttggcag
     9841 agtctgttat aaaagctgat ttagcgatag gttctgagta taccacagaa tcaaaaacta
     9901 ttgaagggaa aacaacaact gaggacaaag aagaccgagt tatcacaagg aaaacaacat
     9961 acaccttggt agcaactcct gcaaatgcgt accagaagac ggtgcaacag ttgactatta
    10021 ctaccgtgag aatgttgagg aaaagtggtt cccaaaacag caacctctac tgagacgaag
    10081 actataacgc gtatcattca ttacgttgat aaagttacga accaaaatgt aaaaaaagat
    10141 gttgttcaac ctgtaacctt aagccgtaca aaaactgaga acaaggtcac gggagttgta
    10201 acctacggtg aatggacaac aggaaactgg gacgaggtta tatctggtaa gattgacaag
    10261 tacaaagatc cagatattcc aacagttgaa tcacaagaag ttacgtcaga ctctagtgat
    10321 aaagaaataa cggtaaggta tgaccgttta tcaacaccag ataaaccaac tccagaacca
    10381 ggtactccaa aaactgaaac tccagtgaat ccagacccag aagttccgac ttatgagaca
    10441 ggtaagagag aggaattgcc aaacacaggt acagaagcta atgctacctt ggctagtgct
    10501 ggtatcatga ccttgttagc tggtctagga ttaggattct tcaagaaaaa agaagatgaa
    10561 aaataataga ttttagaatc taggaacaag gaaaagatca cagatgtggg ctttttcctg
    10621 gttttgagaa tgaggtcttt cgtgaagaat aaaaacgctt acaattacgt tgaactggga
    10681 aactatgaat cctatttttt taaaaatatt tccagaaatc agttgctaaa tagggtaaaa
    10741 attgataaaa taaggaatat ctaaaaaatt ttaaggagaa tctagcaaat ggatttcaca
    10801 tgggcactga agtatgccac tgaatttttg ggaactgcca ttttgatcat tcttgggaat
    10861 ggtgcagttg ccaacgttga acttaaaggt acgaaaggtc accaaagtgg ctggatcgtc
    10921 atcgctgttg gttatggtat gggggttatg atcccagcct tgatgtttgg taacgtatct
    10981 gggaatcaca tcaaccctgc tttcactcta gggcttgcag ttagcggtct tttcccttgg
    11041 gcacaagtgg taccttacat tatcgcgcaa gtcttggggg ctatctttgg ccaagcctta
    11101 gttgtggcaa cataccgtcc attctacttg aaaactgaaa aaccaaataa catcttggga
    11161 actttctcaa ctatttcaag tattgaccat ggtacaaaag aaagtcgata tgcagcaact
    11221 gtcaatggtt tgattaatga gtttgttggt tcatttgttt tgttctttgc agctcttggt
    11281 ttgactaaaa acttctttgg tgctgaagtg cttcaattca tgaaacaaaa ggcaatagaa
    11341 gcaggacaaa cagttgattt ttctgacttg gctattaaag cacaggtggc tccacacact
    11401 gcttcaggac tttctgtggc tcacttggca cttggattcc tcgttatggc tttggtaaca
    11461 tcacttggag gacctacagg acctgccttg aacccagccc gtgacttggg accacgtctc
    11521 cttcatgctt tccttccaaa atcagttctt ggtgagcata aaggcgattc aaaatggtgg
    11581 tattcttggg taccagtagt agcacctatc gcagcagcaa ttgcggcagt agctgtattc
    11641 aaattccttt atctctaaga aatagctcct ttaacatttg agtgagcacc atctataagt
    11701 aagagaggat cagactggtt ctctcttttt gattttttag ggaaatgaaa gaaactctaa
    11761 acaaactcct ctccagcagt ggtttagaag tcttagtggg ctattccagc ttcaatgggc
    11821 tatagtaggt tgcagttgaa ataatagacc cttgtttcta aaacattgtg agaaattggt
    11881 ttgaattctc caatcaaatt gtgcagtttt cattctacta tatattatcg gaatattatc
    11941 ggagatgggt tccctatctt gtaagtttgc tttatagtgg gttgaagttg gaatagtcct
    12001 cccttctttc tcaaacattg tgaggaattg atttaccttc ctcaacaaaa tgttcagttt
    12061 ctatttcatt ttgctataaa ataagcgatt aggggggcta ttcttcgacc tacattgact
    12121 ctgctgagtc ctatgattgt tatcgtttta tctgcaattt tatactcaat gaaaatcaaa
    12181 gggcaaacta agaagctagc cgcaggttgt tcaaaacaca gttttgaggt tgtatagtag
    12241 attgaaacta gaatagtaca catctacttc taaaacattg ttagaaatcg atttgactgt
    12301 cctgatcgat ttgccctatt cttgtttcat tttactatat aaaccagaga ctgtttacat
    12361 tttcagcaag tgagtggatg gataatgctg aaaactcctt gaaggataag tctatttagt
    12421 actttctatt agttagttaa atttttacca agaataattc acaaaaacgt tgtaaaacac
    12481 ttgcaattta gctgaaattt gataaaatag taaggaaagt tagactgtat tgcctactgt
    12541 ctatctataa aatatatttt attggaggct tttactcaaa tggcaaaaga aaaatacgat
    12601 cgtagtaaac cacacgttaa cattggtact atcggacacg ttgaccacgg taaaactacc
    12661 ctaactgcag ctatcacaac tgttttggca cgtcgcttgc cttcatcagt taaccaacct
    12721 aaagactatg cgtctatcga tgctgctcca gaagaacgcg aacgcggtat cactatcaac
    12781 actgcgcacg ttgagtacga aactgaaaaa cgtcactacg ctcacatcga cgctccagga
    12841 cacgcggact acgttaaaaa catgatcact ggtgctgctc aaatggacgg agctatcctt
    12901 gtagtagctt caactgacgg accaatgcca caaactcgtg agcacatcct tctttcacgt
    12961 caggttggtg ttaaacacct tatcgtcttc atgaacaaag ttgacttggt tgacgacgaa
    13021 gaattgcttg aattggttga aatggaaatc cgtgacctat tgtcagaata cgacttccca
    13081 ggtgacgatc ttccagttat ccaaggttca gcacttaaag ctcttgaagg tgactctaaa
    13141 tacgaagaca tcgttatgga attgatgaac acagttgatg agtatattcc agaaccagaa
    13201 cgtgacactg acaaaccatt gcttcttcca gtcgaggacg tattctcaat cactggacgt
    13261 ggtacagttg cttcaggacg tatcgaccgt ggtatcgtta aagtcaacga cgaaatcgaa
    13321 atcgttggta tcaaagaaga aactcaaaaa gcagttgtta ctggtgttga aatgttccgt
    13381 aaacaacttg acgaaggtct tgctggagat aacgtaggtg tccttcttcg tggtgttcaa
    13441 cgtgatgaaa tcgaacgtgg acaagttatc gctaaaccag gttcaatcaa cccacacact
    13501 aaattcaaag gtgaagtcta catccttact aaagaagaag gtggacgtca cactccattc
    13561 ttcaacaact accgtccaca attctacttc cgtactactg acgttacagg ttcaatcgaa
    13621 cttccagcag gtactgaaat ggtaatgcct ggtgataacg tgacaatcga cgttgagttg
    13681 attcacccaa tcgccgtaga acaaggtact acattctcta tccgtgaggg tggacgtact
    13741 gttggttcag gtatggttac agaaatcgaa gcttaattcg atttagttcc cagaagaaca
    13801 attatttaag tcagacacta aaagaatctt gcttggcaag gttctttttt tagatattga
    13861 actaatactc aatgaaaatc aaagagcaaa ctataatata ttgaaactag aatagtacac
    13921 atctacttct aaaacattgt tagaaatcga tttgattgtc ttgattgatt tgtcttgttc
    13981 ttatttcatt ttgttataga aagttagctg cagactgctc aaaacactgt ttttaggttg
    14041 tagatagaac tgacgaagtc agtaacatct atacgacaag gcgaagctga cgcggtttga
    14101 agagattttc gaagagtata atactagact
//
LOCUS       DAVKGU010000046        14044 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKGU010000046 DAVKGU010000000
VERSION     DAVKGU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.046"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(105..542)
                     /locus_tag="VW077_001288"
     CDS             complement(105..542)
                     /locus_tag="VW077_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9455306.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(627..3260)
                     /gene="polA"
                     /locus_tag="VW077_001289"
     CDS             complement(627..3260)
                     /gene="polA"
                     /locus_tag="VW077_001289"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9455307.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPVLLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3516..3866
                     /locus_tag="VW077_001290"
     CDS             3516..3866
                     /locus_tag="VW077_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9455308.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            4081..4197
                     /locus_tag="VW077_001291"
     CDS             4081..4197
                     /locus_tag="VW077_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9455309.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            4190..4423
                     /locus_tag="VW077_001292"
     CDS             4190..4423
                     /locus_tag="VW077_001292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455310.1"
                     /translation="MFNQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFTFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            4550..4831
                     /locus_tag="VW077_001293"
                     /pseudo
     CDS             4550..4831
                     /locus_tag="VW077_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4965..5933)
                     /locus_tag="VW077_001294"
     CDS             complement(4965..5933)
                     /locus_tag="VW077_001294"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9455311.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(6078..6893)
                     /locus_tag="VW077_001295"
     CDS             complement(6078..6893)
                     /locus_tag="VW077_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU9455312.1"
                     /translation="MKKSSILFIFILLLCIDLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLI
                     FLGLALGTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(6918..7415)
                     /locus_tag="VW077_001296"
     CDS             complement(6918..7415)
                     /locus_tag="VW077_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9455313.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLTLGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(7488..8849)
                     /gene="radA"
                     /locus_tag="VW077_001297"
     CDS             complement(7488..8849)
                     /gene="radA"
                     /locus_tag="VW077_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9455314.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSTNVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(8863..9378)
                     /locus_tag="VW077_001298"
     CDS             complement(8863..9378)
                     /locus_tag="VW077_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9455315.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(9380..9823)
                     /locus_tag="VW077_001299"
     CDS             complement(9380..9823)
                     /locus_tag="VW077_001299"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9455316.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(9880..9977)
                     /gene="ffs"
                     /locus_tag="VW077_001300"
     ncRNA           complement(9880..9977)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW077_001300"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(10010..10477)
                     /locus_tag="VW077_001301"
     CDS             complement(10010..10477)
                     /locus_tag="VW077_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9455317.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(10678..11964)
                     /locus_tag="VW077_001302"
     CDS             complement(10678..11964)
                     /locus_tag="VW077_001302"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9455318.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(12195..12431)
                     /locus_tag="VW077_001303"
     CDS             complement(12195..12431)
                     /locus_tag="VW077_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9455319.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRKDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            12697..13558
                     /locus_tag="VW077_001304"
                     /pseudo
     CDS             12697..13558
                     /locus_tag="VW077_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13593..13802)
                     /locus_tag="VW077_001305"
                     /pseudo
     CDS             complement(13593..13802)
                     /locus_tag="VW077_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13968..14041)
                     /locus_tag="VW077_001306"
     tRNA            complement(13968..14041)
                     /locus_tag="VW077_001306"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14005..14007),aa:Asn,seq:gtt)
BASE COUNT         4445 a         3345 c         2355 g         3899 t
ORIGIN      
        1 caacctcaaa acagtgtttt gagcagcccg cggctagttt cctagtttgc tctttgattt
       61 tcattgagta taacacaaaa ggtagcccat cagctacctt tttcttatgc ttcctcaatc
      121 aagcgagtat gttctctctt gatacagcga ttcatcacga tatcatcaca tccaccatca
      181 cgcaaaatct ctttcgcttc taaactttca agtcctagct gtgcccaaaa aatcttggca
      241 tcagctttga gaaaatcacg cgccacatcg ggcagaaatt cactgcgacg ataaacattg
      301 acaatatcta caggaaaagg aatttcagcg aggctagcat aagccttttc acccaagatt
      361 tcgccacctg ccgccttggg attgactggg atgattttat agccccgagc ctgcatttcc
      421 tttgtcactc gattgctggt tgtttcttca cggtcagaca aacccaccac agcaagggtt
      481 ttactcgttg cgagatactg acgaatcacg ccatcacttg gattgataaa ttcttgactc
      541 atagaaaacc tcctttttca tcagtatagc acattttgaa aaggtttgca gaattctact
      601 acaaaaaagg aggactagcc ccctttttat ttagcctcgt accaggttgc cccttcattc
      661 tcatctgcga taagaggaac actgagttga atggcttctt ccatggtttg tttcaccaat
      721 tttttcatct ctaccaattc agatttaggc acttcaagga cgatttcatc gtgcacttgt
      781 aacagcatct tagtctgata accacctgca accaaggctt tatccagctg aatcatggca
      841 atcttgagaa tatctgctgc cgaaccctgg ataggtgagt tgatagcagt tcgctccgca
      901 aaaccacgaa tattgaagtt gcgcgaattg atatctggca actcacggcg acgcttaaag
      961 agggtctcta catagccctt atcacgcgcc tcccgcacca cttcatccat gtagttttta
     1021 atacctggaa aacgttcaaa gtaggtatca atgtaggctt tggcttcctt acgactaatt
     1081 cccaaattat tagacaagcc aaagtctgaa atcccataaa ccactccaaa gttaactgcc
     1141 ttggcattgc gacggtcatt tgcagtcaca ttatcaggac gctcaatgcc aaagacccgc
     1201 atggctgtcg aagtatggat atctgccccc tcttggaagg ccttaatcaa gtgctcatcc
     1261 ttagaaatat gcgccaaaac gcgcaattca atctgtgaat agtcagagct gagtagcaca
     1321 ctatcctccc actctggcac aaaagccttc cgaatcaagc gcccctgttc caatcgggca
     1381 ggaatatttt gcaagtttgg atccacacta gacaaacgcc cggtctgggt caaatcctgc
     1441 acatagcgag tatgaatctt tccatcagcc aaaatccagt cctgcaagcc aattacataa
     1501 gtagattgaa tcttagcaat ttgacggtaa tccaggattt tcttaacaat cggagcaata
     1561 ggagcgagac gctccaacac atccactgct gtcgaataac ctgtcttggt tttcttagtg
     1621 tattctagag gaagtcccaa tttctcaaag agaagcacgc ccaactgctt aggcgagttg
     1681 acattaaact cctcaccagc cagctcgtaa atctcttgag tcagtttttc aatgacaagc
     1741 tcattttcag cctgcatctc aagcaaggtc tctttcttga ccacaatccc agcaatttcc
     1801 atcttggcaa gaacaaaagc cagaggttgc tccatatcat aaagaagctc taattgccca
     1861 ttttcgctga gtttttcaag tagaacaggc tctgtttcta ccaaaacagc aagtttacaa
     1921 gctaagtgtt ccaagaattt ctcacgttca ggaatggcct ttttaacacc cttaccgtag
     1981 aaagtttcat catcaaccaa gtaagtctga ccataaagac tagcgatggt cgcaatttca
     2041 ttgtcctcca cagtcgaaag gaggtattta gccaaacgga tgtcaaaagc aggcgcctgc
     2101 aaatccacac caaaacgttg caaaagaact ttaaccttct taaagtcata aactctcaga
     2161 gatgtttttt ctaagaaatc cttgaaaatc gggtcttgca acagctcaag cttgtctgtg
     2221 gcatagagct tatccccaca agaccaggca aatccaacca aattatccgt atggtaattc
     2281 tcaccaaaaa gctcaaagtg gaagatagac tcttcactca gcatatcttg actgatttgg
     2341 tcaacaatag taaaatccaa accctcagcc acatcagctg acgacacatt taaagcctgc
     2401 ttgagctgtt tgaatcccat ctcatcgtaa aatttcccaa gattttcaac atctggacca
     2461 ctatagacca agtcctctaa accaatcgca atcggtgcct tggtatcaat ggtcgctagt
     2521 gttttagaca aaaaggcctg ttccttgtca ttgatgagat tttccttcat cttagaagtc
     2581 ttcattccat caatattttc ataaatcccc tcaagcgaac catgctccag caagagctta
     2641 atacccgtct tttcaccgac tttggtcacc ccagggatat tatccgactt atcacccatg
     2701 agcgccttga gatcgataaa ctgagctggt gtgaggccca tttcttccat gaggtaatct
     2761 ggcgtaaagg cctcaaactc agccacacct ttcttggaaa tttcaaccac cgtatgctca
     2821 tccgtcagct gaatcaaatc cttgtcccca ctgacaatgg taatatcaaa accatcctgc
     2881 tctgctagct tatccagcgt cccaatgatg tcatccgcct catactgagc cagctcatag
     2941 tgacgaatcc ccatatgatc cagcaactca cgaatgaaag gaaattgctc acgaaactca
     3001 tcaggagtct tggcccgacc acccttatag tccgcataca tctctgtccg gaaggtcgtc
     3061 tttcccgcat caaaagccac caaaatatga ctcggctcaa cccgctccaa caaatgactc
     3121 aacatcaact gaaaaccata aatcgcattg gtatgcaaac cagccgcatt cttaaaacgg
     3181 tccaactgct gatacagcgc aaaaaacgcc cgaaaagcta cagaagaccc atcaatcaat
     3241 aataattttt tcttatccat acacccatta taaaggaaag aatcaaaaaa taccattggg
     3301 aagagctaga gctagtattt ttcaaacttt ttccgaataa atagatagag ccagagaatt
     3361 tagtaaacct agatttaaaa atgtgctata atatagtata ttgaatctat aatagtacac
     3421 cttgactgct aaaatatttc tataaattaa tttgactttc ctgatagagt tattcacatc
     3481 ttatttcaac tcactataga aggaggaata ggaggattct cagacatccg ggcatcagcc
     3541 caactaatga tttgattgct aagaaaatat tcagcaatcc agaaatcact tgtcaattta
     3601 ttcgcgatat gctggacttg ccagcaaaaa atgtgaccat tttggaggga agcgatattc
     3661 acgtattact ctccatgcct tactcggtgc aggattttta taccagtata gacgtcttgg
     3721 cggagttgga taacggtact caagtaatta ttgagattca agtccatcat cagaattttt
     3781 tcatcaatca cttgtgggct tacctgtgca gtcaggttaa tcaaaatctt gaaaaaattc
     3841 gtcagcgaga aggtgatact cactagagct acaaacacat cgctcctgtt tacgccattg
     3901 ctatcgtgga tagtaattat ttctcagatg acctggcttt tcatagcttt agtatgcgcg
     3961 aagacacaac aggtgaggta ttggcgatta ccaacaatgg acaggaaaac catctggtta
     4021 agatggcatt cttggaatta aaaaatacag agaaaccagc aaagacaagg ttcgcaagcc
     4081 atggttggag tttttcggca acaagccctt tacccagcaa ccgcaacgag ccattaccca
     4141 agcaaatcaa ctgctggact acaagagctg gtccgaggag gacaggaaaa tgtttaatca
     4201 actacatatg cgagaagaac aagtcttgtt agcacaggac tatgccttgg aaactgctag
     4261 ggctgaaggc cttgaacaag gattagagcg tgggaaagtt gaaggaaggg cagaaaggaa
     4321 actttttacc ttcctagaca tagtacgcca aggtcttctg acttctgagg ttgccagcca
     4381 gcaattaggt atgtcagtat ctgaatttga ggcactgttg taaaatggct ccataatatc
     4441 catagtgggt aaatccccta tggatattat ggagcctatt ttgtgtagaa aaaaagtccc
     4501 atatgaccta taatgaaaag cgacaaaaca actcattaga aagaatcata tggaacaatt
     4561 acattttatc acaaaattac tagacattaa agaccctaat gtccagattt taaacatcat
     4621 caataaggat acacacaagg aaatcatcgc caaactggac tacgacgccc catcttgccc
     4681 tgagtgcgga aaccaattga agaaatatga ctttcaaaaa ccttctaaaa ttccttatct
     4741 tgaaacgact ggtataccta ctagaattct ccttagaaag cgtcgattca agtgctatca
     4801 ctgttcaaaa atgatggtcg ctgaaacttc tgatgacgta cagtcatatt tcttttcttt
     4861 ttattatatc acagttttaa atctagcttt actagattca ccgctactat ctatttattc
     4921 ggaaaaaaga cgaaaaaacc tgagaatcat ctcaggcttg gtcattaaat ttttttctca
     4981 atatcgaaaa gtggagaaag tggtcgtttt tcatgaatac gtacgatagc atcccctagg
     5041 agatgagcga ttgaaatctg ctcaatctta tcaatcaaac gctcttctgg cagatagatg
     5101 gtatccaaaa caaccaattt cttaatagct gatttttgga tattgtccgt agcaggacca
     5161 gaaagaactg ggtgcgtaca gcttgcatag acttcaacag caccagcttc cgcaagagca
     5221 tctgccgcat gacaaatcgt tccagcggta tcaatcatat catcaatcaa gatacaagtc
     5281 ttgccttcaa ccttaccgat gatgttcata acttcactag tattcatctt atcaacgctg
     5341 cgacgtttat caataatagc gatagatgtt ttcaaaaatt ctgccaactt acgagcacga
     5401 gtcacccctc catggtccgg gctgacaacc acatagtcag aaccaaccat accacgacgc
     5461 tcaaaataat ctgcaatcag aggagcaccc atcaaatgat ccacaggaat atcaaagaat
     5521 ccttgaattt gcgcagcatg caagtcgatg gtcaataaac gatccactcc agctacttca
     5581 agcatatttg cgacaagttt tgaagtgatt ggctcacgcg ctctcgcctt tctatcctga
     5641 cgtgcatacc catagtaagg catgacaaca ttgacagatt ctgcactcgc acgcttcaaa
     5701 gcatctacca taatcaaaat ttcaagcaga ttgtcattta caggcgaact agttgattgt
     5761 aggataaaga cgtgtttccc acggattgat tcttcaatgt tgacctgaat ctctccatct
     5821 gaaaattggc gaacacttga tttccccaac tctatcccaa tctcctgcgc tacacgttct
     5881 gccaattctt tattagaaga aagggcaaac agctttaaat cagaaaaaga catgatttcc
     5941 tccggtatat atgtataact tgtgcttttc acaagatttt ccatctacca ttgtagcgct
     6001 ttttgcacta tttttcaatc aaaaataaaa gaagggcacc atatttgtac ccttgcatca
     6061 ttcttttgaa aaatattcta ggtcatcaac tcattgtgtt tctcaacaaa gcaataagca
     6121 tgataaaaac catagagagc aatagccgta accactggaa tcgctaaagg caactctgtt
     6181 tccaactcca caaaaggaga gttaaacaag aagtgagttc ccaaggctaa acctagaaaa
     6241 ataaggccct gtttcttgcc aaccttctgt cctttatagg ctctgtaaag caagtaaaca
     6301 cctactacag ctagacctga aaaagtccag tgagaggcaa ttcctgagat gatacgctct
     6361 aaaattcgcg aaatagtaaa gtcaaagccc tctggcaaat ccgtacgaat ataaccaata
     6421 tcctcaatca tttggaatcc caaaccggaa gcaattccaa gtaaaaacaa agattttaat
     6481 tttcgcacag gaatcaaagc caaaacaaaa acaagtggca ataatttcaa gggttcttct
     6541 accaaaggag ccacaatagc actttcaaag gcatttaaaa atggactatc tgggaaaaga
     6601 acccccagta aatcatgtat ataagtatta gcaaaactag acaaccagcc tgaaaggaac
     6661 atccctccca ataaagacag aatcaaaacc ttctttggca attcccattt tttcccaata
     6721 cggaagagaa aataaagagc cggaatcatg taaaagagag ctagaaagat agaaactccc
     6781 attagtccat attccgcacc tgacctcgaa ccgtccgtat agtagatggt ttcatactgt
     6841 aaatcaatac atagcaataa aataaaaata aataaaatac tgcttttctt catacacttt
     6901 ctttctaaat gaagtattta taattctacg actgtcatac ttcctgtatc aacattgtaa
     6961 atagcaccag agataatgac atcgtctggt attaggggag actcgataag cagttgcata
     7021 tcctcgcgta cactctcttc tatatcttgg aagggcaaga agtcctggtc tgacacatct
     7081 acacctaatt cctcttttaa atactcctga aaaggttcat tttcaaaggt ctgagcacca
     7141 cagtctgtat ggtgcaatac cacaatctct cttgtcccca tttgttgctg ggaaataact
     7201 agcgaacgaa tcatgtcttc agtcactcga ccacctgcat tccgcaagat atgagcatcc
     7261 ccaagtgtca agcccagagc ttgcgcaacg tgcagacgag agtccataca ggtcacaata
     7321 gctactcttg ttttgggttt aagtggcaga tttaactgcc catgtagggc aacataagcc
     7381 tgattagctt gcataaactg ttcaaaatac gacacgattc cctccttgaa aatttgatag
     7441 tcaaatattt ctcctatctt atcattttta agagaatttg tcacggatta tgcaaagacc
     7501 tttttcaaaa cttcctgaat cgttgtcacg ccaatgacct gaatttcctt aggcagagtg
     7561 attcctgtca aggaattctt aggtacataa atcttagtaa agcccagttt agcagcttcg
     7621 ttgatgcgtt gctcaatacg attcacgcgc cgaatctctc ctgtcaagcc cagttctccg
     7681 acaaaacatt cctgaggatt agttggcttg tctttgtagc tcgaagcaat agcaactgca
     7741 acagccaagt caatcgcagg ttcatccaat ttaacaccac cagcagattt gagataggca
     7801 tcctgatttt gcaagagaag ccctgcccgt ttttccaaaa cagccataat caagctagca
     7861 cggttaaaat caagtcctgt cgtagtacgc ttggcatttc caaacatggt cggtgttacc
     7921 aaagcctgaa cctccgccaa aatcggacgc gtcccttcca tggttacaac gatggaggaa
     7981 ccagtcgccc catccaaacg ctcttctagg aaaacttgac tcggattgag tacctcaacc
     8041 aagccgcccg actgcatctc aaaaatccca atctcattag tggaaccaaa acgatttttg
     8101 accgctctca aaatacgaaa ggtgtggtga cgctcccctt caaagtaaag caccgtatcc
     8161 accatatgct ccaacatacg aggcccagcc aaggttcctt ctttggtcac atgacctacg
     8221 ataaagatgg caatgttatt ggtcttggcc aactgcatga gttcagcggt cacttcacgc
     8281 acctgagaaa cagacccctg cacccctgaa atctcaggag acatgatggt ctggatggaa
     8341 tcaataatga gaaagtctgg ctggatacgc tccacttctg cacgaacact ctgcatattg
     8401 gtctctgcat agagataaaa ctcactatca atatcaccta agcgctctgc acgtagttta
     8461 atctgctggg cagactcctc cccactgaca tagagaactg tccccacttg ggacaactgg
     8521 gttgagactt gtaggagaag agttgatttc ccaatcccag gatccccacc gataaggacg
     8581 agacttcctg gtaccactcc gcctccaagc acacggttga attcctccat ctccgtcttg
     8641 gttcgattga cattggtgga agtcacctca gctagtttca tgggcttggt tttctcacct
     8701 gtcaaggaca cacgcgcatt cttaacttcg gcaacctcaa cctcttccac aaaagaagac
     8761 caagacccac agttggggca acgtcccaga tatttagggg aattataccc acaattttga
     8821 catacaaatg tcgctttttt ctttgcgatg acaaacctct ttctatatct ctaactcaca
     8881 ctcaatcact tggcaaaaat caatcttctc atttggcaca aactggcgca tgagcattcg
     8941 atgagcaaca actaccacag tctgatgttc tcgatactta gacatacatt ctagaaaccg
     9001 agacttcatt tccgtagctg tctcatattg aataggacta ttaggaagca actccccctt
     9061 gttttctaaa aacagtcttc tagctgtttc aaagttttct attcctgttt tatagacctg
     9121 ccattcatgt aataaaggct ctactcttaa aggaagaccc gtagcacaga ccacatacga
     9181 agccgtttct aaagctcttg tgactgcaga agatacgatt atttcagctg acgagagtaa
     9241 aggatttttg ctcaatttct ggacttgctg ccgtcccatc tcagacaagg gtgccaaatc
     9301 tatcccaaat cctatataag aacgctcctc taactcacgg taatctggct ccccatgacg
     9361 tacaaagata atcttcattc tagtgccctg tcgatccaaa tccaccagtt cgaacgccat
     9421 cagctgcatc tccatctgca attaagaaag tagcaaaaac agcctggaca atacgctccc
     9481 caacttcaag aacaacctct tggtctgtga tattcttcat ctgcgcaaaa atatgccctt
     9541 catttccagg atttccataa taatccccat caatgacccc aactgagtta attaaaacca
     9601 agcccttctt acgaggattt gaagaacgat catagaggta gagaacctca gtcggctgca
     9661 tataagcctt aacccctgtc ggaaccaaga caatctctcc tggcgcaaca actgtacgca
     9721 cagcaacctt taagtcgtaa ccagccgcat gcgctgtctc acgcttgggc aataaatttt
     9781 catctgtaaa actcgaaacc aattcaaaac cacgaatttt cataattttc tcttttctat
     9841 tatcatttat tctagattat tctatcttat ttattcggaa aaagcacgaa aaaaagagca
     9901 cacaattcaa atcgcttagg gctgctggat tcctcccctg acccgcttca cgcagaactg
     9961 ttgctccact atttattata tcacattcct attcatttta aaagcaaaat tattttttcc
    10021 gtctatttct aaaaaaatcc tgcatgatag ctgcgcattc atcttccaaa attcctattt
    10081 caacctccac acgatgattg agacgctcat ctgtcaagat atcgtacaaa ctcccagcag
    10141 cgccaaattt ctggttttta gccccataga ccacatttgg aatgcgggca agcccaatcg
    10201 ccccactaca catgacacaa ggttcaatgg tcacaaaaag tgtgcaatcc agcaagcgcc
    10261 agctctcctc actcaagttc gcatcctcta tagccataat ttccgcatgc ataaccgctc
    10321 gctgtaattc ctcacgcgca ttatgcccac gaccaatgat ttccccatct ttgacaatca
    10381 cacaaccaat tggaatttca tcgtgttcaa gagcaatctc agcctctctc aaagcctccc
    10441 tcataaagac ttctttttct tcaagcgtat aatacatctc ttttctcttt tcctacttat
    10501 cgattttatt attatatcat gaatcccaac acaaaaaaag ccaccgaatg cggtgactct
    10561 atagggagat tattatgaaa aagaaaagtt taggatattt gttacaacaa gttaggaggt
    10621 cttcttgtaa ccgtctatag tatacccgac ctatcttaaa caaatcttaa aaatctctta
    10681 ggaccaaaca ctttctaaaa tatttgtttg ttcacgacca ggacctactg agaaagtaga
    10741 aatacgaacg ccaaccaatt cactcacacg acgaacatag ttacgcgcat tctcaggaag
    10801 atcttccaaa ttgcgaactc cggtaatatc ttctgaccaa cctggtaact cttcatagat
    10861 aggcttgcaa cgtttcaatt gctcaagact agctggatag tagtcaatac gttgaccgtc
    10921 aagatcatag gccacacaga ttttcacagt atccaaaccg ctcaaaacat caatagagtt
    10981 caaagaaagg ttagtaatac cagaaacacg acggctatga cgcatcacaa ctgagtcaaa
    11041 ccaacctaca cgacgtggac gaccagttgt tgtaccatac tcatgaccca cttcacggat
    11101 acgttctccc acttcatcaa acaactcagt tgggaaagga ccatctccta cacgactcgt
    11161 ataagcttta catacaccta caaccttgtc aatcttgctt ggaccgacac cagaaccaat
    11221 tgtcacacca ccagctacag ggtttgatga cgtaacaaat ggataagtac cttggtcgat
    11281 atctagcata acaccttgtg caccttcaaa aagcacacgt ttgccattat caagcgcatc
    11341 attcaagata acagatgtat ctatcacgta tttcttgatt tgttgaccat attcgtaata
    11401 ttcttcaaaa atatcatcga aaacaatcgc tttactgtca tacaattttt caaaaagacg
    11461 attcttttca gcaaggttac gttctaaacg ctcacggaaa atatctttat ctaaaagatc
    11521 tgcaatacga attccaacac gagcagcctt gtccatataa gctggaccaa ttcccttaat
    11581 tgtcgtacca atcttattgt cgcccttagc ttcttcttgc aagcgatcca actcgatatg
    11641 ataaggcaaa ataacatgcg cacgatcaga aatacgcaag ttatcagttg ttacaccttc
    11701 ctcatgaaga tagctcaact cttttacaag agatttagga tttacaacca taccattccc
    11761 aatgacagat attttttcag ggaagaaaat cccagatgga atcaagtgca acttaaattt
    11821 cttaccgtca atcacaatcg tgtgaccagc attatcacca ccttggtaac gtgcaatcac
    11881 ttctgcattc gctgaaagga agtctgtaat cttcccttta ccttcatcac cccattgggt
    11941 acctacaaca acaactgaag tcataatttt gtctgagccc tcaggctctt cctttctcac
    12001 atacatggca ggactctcac ctgcaattat atcttacaat ttattataat aaaaaatcgc
    12061 ctttttatca agaagaaaca atagaaagat ttgctatttc caactattaa aaaatgattt
    12121 agaaaaatta ctagctattt actattatct ttccataaaa gagtaaatta gttcggaaat
    12181 ttactaaaat tacctcaaca agaaataaaa ccccgattca ttaccaattt ttcaagatac
    12241 aaacgataag caacacgata atggtaaaca ataaaatcct tacgacaacc aatgccatat
    12301 ctcactaaat aataaattaa aaatttaaaa tgaacatgtt cccagtcaaa attatcacca
    12361 aatgtaggac catactcttc ttcaatacta tcatagaaat tagccatctg ctcataaatt
    12421 ttttgtaaca taatcaaata ctccttttct tttttataaa cttattctaa caaaaaattt
    12481 tacacattca ctatcaattc ctgaattgtt aaaataccag cctcaacaag ataaaaatag
    12541 gaaaagttga caaaatagaa acaaatttgc tacctaaatt tcaaacaatt aaagttctaa
    12601 aaagaccgcc ttaaaacttt tgggtaaatc ctattagaac tataatattt ttatataggt
    12661 attatggttc tgattttcta gtataaaaaa attcaaacta gaattcttct tagaaaaggc
    12721 cgttttaagt gctatcattg ctcaaaaatg gagctcgctg agacttctat cgtcaagaag
    12781 aatcatcaaa ttccttgtat catcaaccaa aagattgctc agaagctaat tgaaaagact
    12841 tctatgaccg acattgatca tcagttgtct atttcaactt caactgtcat tcgcaagatc
    12901 aatgattttc actttgagca tgatttttcg cgtcttcctg agattatgtc ctagtacgtt
    12961 gaaacagtcc agggagtgac tatttcaatc gggagatgga gatgagcttt attgctcagg
    13021 actttgataa gctcaatatc ataactgttc ttgagagcag aacacaagcc atcatccgaa
    13081 atcccatgga tacaaggcta tcaagcgcta ctggaaactc attcaacagg atagtcgtaa
    13141 actgagtgat aaacattttt atcgtcctac ttttcgtatg cacttaacca ataaagagat
    13201 tttaaacaag cttttgagtt attcggaaga cttgaaacac cactatcaac tctatcagct
    13261 cttgcttttt cacttccaga ataaggaacc agagaaattt ttcggactta ttgaggacaa
    13321 tcttaagcag gttcatccta tttttcagac tgtctttaaa accttcctca aagataaaga
    13381 aaagattgtc aacgcccttc aattacctta ttccaacgcc aaactggaag ccaccaataa
    13441 tctcatcaaa cttatcaagc acaatgcctt tggttttagg aactttgaaa acttcaaaaa
    13501 agaaaggacg aaatttgtcc tttctaaatc ttcgctttct tcaacccact acagttgaca
    13561 aagaaccgaa aaatgtctcg cctctaatat agtcaattga aacaagaaca agacaaaaga
    13621 gcctcgtaaa aggtattgca acttggtaat acctttttga ggtgcttttt gatatgagcc
    13681 catgttttct taataggatt gtactcaggt gagtagggag gaagaggtaa aagttcatgc
    13741 ccaaactcct cgcataaaag ttctagctta cccattctat ggaatcttgc attgtccata
    13801 atatagtaga ttgaaactag aatagtacac ctctacttct aaaacattgt tagaattcga
    13861 ttttactgtc ctgatcgatt tgtcctattc ttatttcatt ttactataac acgaaataga
    13921 ttattattac ataacaaaaa agaggtctaa acctcttaac taaattactc cgccagtagg
    13981 actcgaacct acgacatcat gattaacagt catgcgctac taccaactga gctatggcgg
    14041 atta
//
LOCUS       DAVKGU010000047        14034 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKGU010000047 DAVKGU010000000
VERSION     DAVKGU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.047"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(55..261)
                     /locus_tag="VW077_001307"
                     /pseudo
     CDS             complement(55..261)
                     /locus_tag="VW077_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(323..772)
                     /locus_tag="VW077_001308"
     CDS             complement(323..772)
                     /locus_tag="VW077_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9455320.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYNYIKTHRVS"
     gene            complement(774..1532)
                     /locus_tag="VW077_001309"
     CDS             complement(774..1532)
                     /locus_tag="VW077_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9455321.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1647..3626)
                     /gene="pknB"
                     /locus_tag="VW077_001310"
     CDS             complement(1647..3626)
                     /gene="pknB"
                     /locus_tag="VW077_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9455322.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIILTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3623..4363)
                     /locus_tag="VW077_001311"
     CDS             complement(3623..4363)
                     /locus_tag="VW077_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9455323.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4378..5691)
                     /gene="rsmB"
                     /locus_tag="VW077_001312"
     CDS             complement(4378..5691)
                     /gene="rsmB"
                     /locus_tag="VW077_001312"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9455324.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFANGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5684..6619)
                     /locus_tag="VW077_001313"
     CDS             complement(5684..6619)
                     /locus_tag="VW077_001313"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9455325.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6632..9028)
                     /locus_tag="VW077_001314"
     CDS             complement(6632..9028)
                     /locus_tag="VW077_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9455326.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QESNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKVYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9094..9408)
                     /locus_tag="VW077_001315"
     CDS             complement(9094..9408)
                     /locus_tag="VW077_001315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9455327.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9433..10059)
                     /gene="gmk"
                     /locus_tag="VW077_001316"
     CDS             complement(9433..10059)
                     /gene="gmk"
                     /locus_tag="VW077_001316"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9455328.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10189..11802)
                     /locus_tag="VW077_001317"
     CDS             complement(10189..11802)
                     /locus_tag="VW077_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9455329.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSHEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11993..12247)
                     /locus_tag="VW077_001318"
     CDS             complement(11993..12247)
                     /locus_tag="VW077_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9455330.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(12251..12505)
                     /locus_tag="VW077_001319"
     CDS             complement(12251..12505)
                     /locus_tag="VW077_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9455331.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12743..13840)
                     /locus_tag="VW077_001320"
     CDS             complement(12743..13840)
                     /locus_tag="VW077_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9455332.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILARLGIDSLAFGTEEVLD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAIWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVEAVATKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETS"
BASE COUNT         3727 a         3336 c         2777 g         4194 t
ORIGIN      
        1 acttgtgaca atcgcttgag ccttcatagc gtggcatttt ttttaccaga atcattcgct
       61 aattcttttt ttagggcgat tgatttgtac ttccgtcgca tcaatcatta ccgtgtcctc
      121 agaactgaga ggagttcttg aaatcgtaac accactttga acaagagtta cttcaaccca
      181 ttggctccga cggattaagt tgctttcgtg gataccaaaa tcagccgcaa tttcttcata
      241 agtgcggtat tctcgcacat atagaaagcg ttgttaattt atttatctca tttttcagaa
      301 aattctttta tttctgtaaa gtctacgata ctcgatgtgt ctttatataa ttatacagtc
      361 tgagaatcac tgttccgcta gccattccaa tagagattac cagagccaac atgacaacca
      421 aggtcgcact tgccagtgct ttattatagt cccctgtcac aaaaaaggca gttgttcggt
      481 aggagagata acctggaacc agcggtgcca aaatggccaa gataaagacc acagcaggtg
      541 tcttataaag aatacttaaa atctggctga cacaagaacc aataatggct gcaatgaagg
      601 tagctacaat gacattggtc ggttccttga gcaagagata gattagccag acagtcatgc
      661 ccaaaatccc tccaggtaag agcatagacc gttgcacatt gagtacgatt aaaaaagtga
      721 taatggcaag aaaacttgct actgcttgta ataaaaaggt tgttagtgtc atattagttc
      781 atcaatacca aggcgacaga agttcctgcc cctaaagcga gggtaatgag cagggattca
      841 aacatcttac tcataccaga gtttatgtgg ttggtcataa tatcacggac cgcattggtc
      901 aaggcaatac ctggtacaaa cggcatgacc gcaccagcta taatcaaatc tgccgttgaa
      961 ggaaaacctg tgtagcgagc ccaaaactgg gcaattatcc caaagacaaa ggctccagca
     1021 aaggctgtca caaagggaat tcggataaat ttttccacat agagggaaaa ggcaaaacca
     1081 aataaggtcg ccactcctgc cccaagtgcg tcgtagatat ttccgctaaa cataactgaa
     1141 aagaaaggag cactaaaggt cgcagccaga gttacctgca acttagtata gggaaggggt
     1201 tgagcttgca aggccgtcaa ttgcttaaag gctgtttcta agtcaatctg ccccccaact
     1261 agctgacgag aaatctggtt cacatcgcag actttttcga tgttataaga agaggaggtc
     1321 acgcgcttca tgcgcgaaat attggtattt tcaatagaga aaaagatagc ggcaggcatg
     1381 gcaaggacat tgcaatccac aatcccctgc gaatgcgcga ttcgaatcat ggtatcttct
     1441 acacgatgga tttctgagcc acttttaagg agaatagtcc ccgctagcat aatcacatca
     1501 atgacggcat ttaattcttt tgattcttcc atgctttcct ccttttatca actccctcta
     1561 ttctatcaca aatctggact caaaaaaaat ctttgccatg aaatcatgac aaagattgat
     1621 tactcatttt gattatccat ctacttttaa ggagtagctg aagttgtttt aggtttgtag
     1681 attgaaatct tgactctagt cttattgagg tctacctttt cacctgctct aggactttgt
     1741 tcaacaacca tgccttctgc actacctgca ggcgctgtcg tcacttctac aacttctata
     1801 ttagcttcct taatcccaac aatttgaatc aaattgttct tagtaaactc caagctggaa
     1861 ccaatgtaac tcggcatggc aacacttgta acttttttag ctactgtcaa gacaatttga
     1921 gtaggtttac tcacatcata agtcgttccg gcacctggac tttgtttcat aatcgttcct
     1981 ggttcgcttt cgctggactc ttcttcctct atcttaatca aattctcagg aaccttcttc
     2041 tgcttgagtt ctgagattac ttctgtagag ttccgtccaa tatagttccc taattgaatc
     2101 gtcgtagctt ttttagctac tgtcaaaata atttgagttg ccttgctcaa gtcataggtc
     2161 gtaccttctg gtagactttg cttcaggacc gttccagcct cactctcatt cgactcttct
     2221 tcctcaattt taatcaaatt atctggaact tttttctctt ttaattccgc gataacatca
     2281 gaagatttcc ggccgacata attactaatt tggaaggatt gtttgcctga tgagacaacc
     2341 agattgattt tcgttccttc ttttcgacca gttccagcgc caggatctgt acggataatc
     2401 cgcccttctt ccaccttttc actagcctct gtcttctcct caccaatctc aaaattggct
     2461 tttttgagcg ttgccttggc ctctgcaact gtctgacctg ccacatctgg aatggcaatg
     2521 gttgcaggag ttctggatag tatccaaata agagaagctg ccaccaatac aaggctggcc
     2581 aacaaaatca ggtaacgcat cttaaatcta tgttttttcg gtgcttgtgg ttggtaagtt
     2641 tcctctgtca cagcctggct tgggtttttg attgatttgt gttctgtctg cgcttgaacc
     2701 ttaggaatag atgtcaaggt actctgagaa accttcggca aggtcttggt atctgccttg
     2761 ctcgtttcat caaagattaa cttactttca tttctacgat tgtaggacaa gctactagac
     2821 aagtccacat acatctctga aaccgagcgg tagcgattgg tcaacttttt agcagttgcc
     2881 ttgataataa cattttctaa agcctgaggt acagatggat tttctgcaat aacggacggc
     2941 aggggtttct ggaaatgctg gagggcaatg gtcaccgcgc tatccccgtc ataagggata
     3001 tggcctgtca acatctcata gaaaataatc cccatggcat agatatcact ctgcacagtc
     3061 gccttagaac cacgcgcctg ctctggtgac aagtaatgaa ctgagcccaa catcgagtta
     3121 gtctgggtca gacttgtctc tgcaaaggct acagcaatcc caaagtctgt gaccttggca
     3181 gtcccatctg gtgtcaaaag gatattttga ggtttcaagt ccctgtgaac aattcctcga
     3241 gtatgggcca agcgcatagc caagagaatt tgtcccatga tacggactgc ttcttcatta
     3301 gaaagaggat aatgttcctt gatatagcgt ttgaggtcca gtccagccac atactccata
     3361 gctaggtact gttgaccgtc ttcctcgcca atatctgtta tccgaacgat atgaggatgg
     3421 tctagatctg ccatagctct cgcttcacgc tgaaaacgag ctacagctat cgggtccgtc
     3481 tggtagttgg tcctcagaac cttcactgcc acttcttccc catctaagat taagtctttg
     3541 gctaggtaga catccgccat gcctcctcga ccaatctgtt tgacaatccg atagcgtccg
     3601 gcaaaaatct tgccgatttg gatcattctg catcctcctc gttcatagaa acaagggcaa
     3661 ccgtaatgtt gtctaaacct cctgcattgt tagcaaaacg aacaagtgtc tccgttttat
     3721 ctgctaaagg aatatcactg gttacaatat cacgaatctc actgcctgaa atcatgttgg
     3781 tcaagccgtc actattgagc aagagatagt cacctgactc aaggataact gtcccaaaat
     3841 caggctgaat ttcatctttt tgcccaatag actgggtgat aatatttttt tgcggatgag
     3901 cttctgcctc ttctggtgtc aattgaccag ccttgagcaa ttcattaacc aaggaatgat
     3961 cgctcgtcaa ctgatggtat tcttctccac gaatcaagcc gatacgcgaa tcaccaatat
     4021 gagcatagat agcctgatta tcaataatag caaggacttc caaagtagtt cccatgcctc
     4081 tgtaagcttc atcctgacca agctggtgaa tcttttgatt ttcaatttct aggtaatggg
     4141 cgaaccattc acgcacttca ttgactgtat cgatctgagt atcaacccaa gctacaccca
     4201 ggtctgtgac tgccatttca ctagcgatat tccctgcgcg atgacctccc atcccatcag
     4261 ctaaaataat catggtacgt ccagctctat tgacatagtg gttgacatag tcttggttat
     4321 ttgttcgttt ctgaccaaca tctgttaata atgaaatttc catgtgtcag ttccttccta
     4381 atccgatatc ttgcgaaatt gactgatgaa gaatccatca cttccataca attcaggtgt
     4441 aatgaggata cagccgtctt tcatgatatc cttacattca tgttctagtt ttacctgctc
     4501 gaactcggga tgactctcta aaaaggcctt aacgacttga aaattctcct ctgagacgat
     4561 agtgcaggtg ctataagtta ttataccacc tttgcctagt atttgacaaa cactacctaa
     4621 tatttctaac tgaatttcct gcaaggacgc gaaatctgcc gtttctttat tgtatttgat
     4681 atctggtttt cggcgcaaaa gaccgattcc tgaacaagga gcatccacca aaatcttatc
     4741 aaaggaatcc tggtcaaaaa actcatgcac ctttctggca tccaattttt gagtttgaac
     4801 ccgatcttca actcccagac gttgggcatt ttcttgaatt aaatccaact tatggtcgta
     4861 caagtccaga gcagtaacct gacctgtcgt aagataagag gctatatggg ctgttttccc
     4921 acctggagcc gcacaggcat caagcactcg ctcatcacct tgtaaatcaa gcgtcggagc
     4981 aaccagctga ctggactcat cttggatggt aatggctcca ttcgcaaaca aattatgccc
     5041 tgcaaaatgc ccctgctcct taaccagacc agtggttgct aaaagggaat tattcgcctc
     5101 caacaaggct tggatttcct cttttcgact taggtctgtt acacgaatac tggctttgtt
     5161 tcgcactaac aggctttcaa agatggcttt tgctctctcc tctccgtatt cttccttgag
     5221 tttggcaact agccaaactg ggagagaata ggcaatggag tcacgcttgt tttttcgctt
     5281 gatgctagca atatctggcc agccttcacg caagatacgg cgaaggacag cgttgaccaa
     5341 tttttcactg ccttttttac ggagtttggc caattccact gcttcattga ccacagcatg
     5401 atctggaatc ttgtccaaat agcggagttg gtaggcactc atgagaagaa ggacatagag
     5461 ccagctgtct aactggtctc tgtcttcgat aaagtgggat aggtaccatt ccagagtcag
     5521 tttacgggct accgttccat agactagctc ggtcactaag cccttgtctg ctgcagagag
     5581 ctgactcccc ttgaggtgtt tatttaaggc aatatttgaa tatgcttgat tcacaaaaac
     5641 atcctctagc actgctagag ctaaacttct agccgtttct actttagtca ccaaatcgtt
     5701 ctcctacagt caatgtacgt ccaactccgt tgaggaagga agcaatgtcc atcttaggct
     5761 taccagctgg ctgcacttgt ttgagggata gagccccttc agccgttgcg acaatcaatt
     5821 ctttcttgca gatagagaga atctcacctg gatttccctg accttctact ggtagggctt
     5881 cataaatctt aaagcggtcg cccttaagga aagtatgggc aacaggccag gggttcattc
     5941 cacgaatttg gttaaagagt tgacgattgg ttttgttcca gtccagtttt tcttcctctg
     6001 gctttatatt tggagagaag gtaacctgac tcgtatcctg cggttcaggt ttgatatcac
     6061 cagcaatata ggcaggcaga gtgtccaaaa gcaaatcacg accaactagc gccaattttt
     6121 caaacaaggt gccaacattg tcctcatctg tgatcggaat gctgcgacga gaaatcatat
     6181 ctcctgcatc catttcctta accatttcca tgatggtcac accagcttcc tcatcccctt
     6241 gaatcaaggc ataatggata ggcgcaccac cacggtgtct aggaaggagg gaggcatgaa
     6301 cgttgacagc aaagtccatg ctatcaagga gtttgcttgg gagaaactgc ccaaaagcag
     6361 cagtcacaat tccatctgct cctagcttca taagttcttc catctctgga cttccagata
     6421 atttttcagg ttggtagata gatagtcctg cttccttggc agcctgcttg actggggttt
     6481 cttggataac ttttttacga ccaacagcac ggtctggctg ggtcacaacg gctagaattt
     6541 cgtaacggtc atctgtcaaa agtcctttta agactgttgc tgaaaagtcg ggggtcccca
     6601 taaagattag ttttgtcata tcttctcctt cttataaaaa ttgctgcggc tcatggtcaa
     6661 tgctgagacg gagctcacta ttttcccgtt cttgagtcaa ggctaaaacc tggttgaggg
     6721 tcggccccag ctcatcttct aaacggtatt taattaaaat ctggtaatga tagaggttgt
     6781 gggtacgggc aatcggtttt ggcgttggcc ccagaatggg actggtctct gacaagcctg
     6841 accgcaaaat gttcatgact tcataggcac gtttgaaaac ctcttcttct ttcttgtgag
     6901 aaagggtaat accaatcgtg aaatagtaag gtggatagcc gagttgtcgt ctgattccca
     6961 tttcataggc ataaaagcct tcgtaatctt gatccttggc aaatcgaata gcatagtgct
     7021 gcggattgta ggactgtatc aagacttgac ctgccttttc agcccgacct gctcgacctg
     7081 ccacctgagt caagagctgg aaggttctct cagaagaacg gaaatcaggc agattcaagg
     7141 ccgtatccgc atttagaact ccgactaggg taacattggg aaaatccaaa ccctttgcaa
     7201 tcatctgagt accaagtaaa atatccgctt cccctcgccc aaactggtca agcaaggctt
     7261 ggtgactgcc tttctttcga gtcgtatcca catccatcct cagaatgcga gcttggggaa
     7321 agagttctgc tagctcatca taaaccttct gagttcccgt cccatagtaa cgaatactgt
     7381 ggctcttaca gttaggacag acctgaggaa tatccttcga gaaaccacaa taatggcagt
     7441 tcatagtctt ggtatccata tgcaaggtca gagaaatatc gcagttggga caagtatcca
     7501 ccgtcccaca ctcccgacac atgacaaagc tagaataacc acggcgattg agcatgagaa
     7561 ccacctgctc ttttttaacc agacggtctt ggatagcctc tagcaaagga ggcgtaaagt
     7621 ttgacgtctc attttgtccg atatagtctc gaaagtcaat cacttgaacc tcagggattg
     7681 tagccaaagg attggcacgt tgggttagac gtaagtgttg atagacgcct ttgccagcac
     7741 gcgcccggct ctctaagctc ggcgttgcag atccaagtac cagagttgct tgattatact
     7801 gagcccgtaa aatagctacc tctctggcat ggtaacgggg attgctttcc tgcttataag
     7861 ccgcttcatg ctcttcatca ataatcatga cacccagatt tttcagagga gcaaagatag
     7921 cagatctggc accaacaaca acttgggcat cgccacgctc caccttgcgc cattcatcat
     7981 acttttcacc attggataat cctgagtgaa gaatggctac cttgtcccca aagcgtgcta
     8041 taaaacgctc ggtcatttga ggagtcaagg aaatctcagg taccagcaaa atagctgtct
     8101 tgcccttatc cagggcacct tggataatct gcaagtaaac ctcggtcttc ccacttcctg
     8161 taatcccttg aagtagaaag ggaggttgag aactgccaat agaactcaca accgcatcac
     8221 gcgcctgtct ttgttctgga tttaactcca aaggtctact tgcttcaatt ccttcaaaat
     8281 aagcagccga gcgttgaact tccttttgga ctatggtaac agcaccttga tccacaaaga
     8341 agttgacttg ctctcgcgag taggactcca acaagctagc caaggaagcg ctctctggat
     8401 gagacagcag ataatctctc agttccaact ttttcttggc acgtgtagaa atctcaacac
     8461 cttctaattg agcatggtca acctcatacc aagactgggt cttgaccttc ttttgatcga
     8521 ctgcctgata ttccagacca agcaggcctt ttctagtcaa acgcatcatt tcagcttgct
     8581 tacctaaatc cagagaagaa aaggctagcg aatcttctga accaaacagg cgtactcgtt
     8641 cttcctgact caagccttcc agaggataga gaatcttgtc atagctagaa ttcagaaatc
     8701 ccggaagcat ggccttgagg atagagattt tgtaggagaa gacagatttg cgtaactcct
     8761 cagccagcca gagttgttct ggcgtgagaa caggagaaaa atccagcacc tctgcaatat
     8821 cttttaaatc ttgctccatc tcttctccat ctgattggga ctccaaacca agaacaatcc
     8881 cttgaatcag gcgattaccc ttaccaaaag gcacatgaac ccgcatccca acttccagca
     8941 ttccctcaaa ttcctccgga atcctgtaac tatagggctg gtccgtctgc atcaagggca
     9001 catctacgat aatcttagct agggccatct tctcacctcc tccttgtcag tacattcttg
     9061 caatagaaaa aataagattg agtcccccca accttaaatt ttttcaccat cttctttttc
     9121 tttagcaatt tgctctttga ttttcttttc ttcttcttct ttgcggcgtt tttcttcttc
     9181 gatacggcga cgcactgctt cacgttttcc ttctggatct gggtgaattg taacgtttcc
     9241 tgattcgatt tcttctaaag cgcgaagagt tgatttttca gacttgaaac cttgagttgc
     9301 tggggcacct gcttccaatt cgtgggcacg ttttgcttcc aagattacga gtgaatattt
     9361 tgaaggaacc ttgtcgagca aggtatcaat agagggtttt aacatcattt gcttgtacct
     9421 attttctaaa ttttatcggg tagttggaga ttttggtaac atctcctgat agtgaccaat
     9481 gacacgatcc acacagaagt gttctgcttc aatcacacat ttgacacgtt cagcagctag
     9541 gggcacctga tcgttgacaa tcgcataatc atactcacgc atgagggcaa tttcttcctt
     9601 ggccttttcg attcgttggg caatcacttc tgcactatct gttccacgac ctaccaagcg
     9661 atcttgcaat tcatccaaat ctggtggtgt caggaagata aagacagcat ctggaacctt
     9721 tttcttgacc tgaagagcac cctgaacttc aatttcaagg aaaacatcga ttcccttgtc
     9781 caaggtttca ttgacatagg tcagaggagt tccatagtag ttgccgacat attctgcgta
     9841 ttccaacatc tgtccttgac gaatcagctc ttcaaattct tcacgagtac ggaagaaata
     9901 gtcaacaccg tccacttctc caggacgttg tgcgcgtgtc gtcatcgata cagagtattg
     9961 aaattggttt tcagaactct caaaaatctc tcttctaacc gttccttttc caacccctga
    10021 aggaccagaa aaaacgatta gtaagcctcg gtctgccatt gtgtctcctt ttagtcaatc
    10081 tgtgaaataa catttctcta gaataatggc aaaaagccag attatccttt acagtctttc
    10141 tatctagtgt aacaaaaaag cagtaatttt tcaactgctc tttcttattt atttagcata
    10201 atctactgca cgaagctcgc gaatcacggt taccttgata tttcctggat aatcgagatt
    10261 gttttcaatt ttcttacgaa ctttgtgagc caagattgtg actttgtcgt ccttgatttt
    10321 tcctggattg accatgatac gaatttcacg tcctgcttga agggcaaagc tagtttgcac
    10381 tccttcaaag ccgttagcaa tttcttccaa atcatggaga cgcttgatgt agctttcaag
    10441 agactcacta cgagcacctg gacgggctgc gctcaaggca tctgctgcag cgacgataac
    10501 tgctatcacg ctctcagctt caacatctcc gtggtgacta gcaatcgtat tcaccacaac
    10561 tgggggttcc ttgtacttac gggccaattc cataccgatt tcaacgtggc taccttcaac
    10621 ctcatggtca atggctttcc cgatatcgtg aaggaatcca gcacgacggg caagagccgc
    10681 attttcacca agttcgctcg ccatgatacc agccaactta gcaacctcaa tcgaatggcg
    10741 caaaacattt tgtccatatg aagtacggaa ctgcaaacgt cccataatct tcatcaagtc
    10801 tggatgaagg tttggcgcac caatttcata ggcagcagcc tcaccgtatt cacgaatctt
    10861 attgtcaatc tcttgacggt ttttctcaac caactcttcg atacgagctg gatgtatacg
    10921 accatctttg agcaacattt ccatagtcat acgggcaatc tcacgacgaa tcggatcaaa
    10981 tcctgacaag gtcaccactt ctggtgtatc gtcgataatc acatcgaccc ctgtcaaact
    11041 ttcaaaggta cgaatgttac gaccttcacg accaataatg cgtcccttca tagtatcgtc
    11101 tggcagatga actgttgagt ttgttgactc cgctacatat tcaccagcga tacgttgcat
    11161 agcttgaacc aagatgtcct tggccatttt gtcagaacgt tccttgacct cttgctcagc
    11221 ttcgcgaatg cgactggcaa tctccctggt caagttttcc tctgtctgag ccaagataat
    11281 atctcgtgct tctgcctgag acagagcacc aatacgctct agttctgctt ctttttgtct
    11341 ttcgacttcc tctaattgct cttcacgcgc atcaaggttt ttcgctctat cagaaatact
    11401 ttgttctttt tgttcaagtg tttgttcttt actcgtcaaa ttgtcgtcct tacggtcaag
    11461 gctagtagct ctctctgtca aacgactttc gatttgtttg agttcttgac gttctgattt
    11521 aaattcagcg tccacttctt cacggtattt tctggcttct tctttggcct ccaatagtgc
    11581 ttctttttta agagacttgc tttcacgttt ggcttcatta acaagtaaat ccgcttcacg
    11641 ctcagcttgt ccacgtaaat tagttgcttc ttgttcagca tttaaaagca tcaactctgc
    11701 agcttcatga gatgatttca tcttagctga gatgctgaca tatccaatga ctaaaccaat
    11761 gatgacggca aaaacagcaa tcgcaagcga catgatctcc atgtttttac ctcattttat
    11821 tgttattccg aatgacatac attcttttac attctaccat aaaaaagtga ttttcacaaa
    11881 cctaaaatag aatatgtttt ggggaatttg gaacacattt gccaaaataa acttgttttt
    11941 tagaaatagt agtttagtag agacttgaga aaaagcctac ctttcaatag acttagtaat
    12001 gatctttaaa ggacaagaaa gccacgctat ctccatccat catataaatc aagcgatttt
    12061 ctgcatcaat acgccgtgac caggctcctt ggtaatcata tttgagtggc tctggttttc
    12121 ctattcctgt aaatggatcg cgttgaatat ccttgattaa tttattgatt ctttttagtg
    12181 tttttttatc ttggttttgc cagtagcaat aatctgccca cgcatcttct gtaaacttga
    12241 gtagcattca tcactcctca atcacatgga cctgagtgct tccagcacgg acttgagcca
    12301 ttcctcgcaa aaccttgtct gataactcct tattttgggc gattctcagg gtttcctgaa
    12361 tactgtccca ttcgctcttt gaaagaacta caatgtcctc atctggattt ttattgacca
    12421 ccgtcaaagg ctcaaattca tcatttacct tcttcatgta gtcctttaaa tgatttcgga
    12481 atgttgagta aaggactgct tccataacca tacctcgttt tagctctttt ccactattat
    12541 acacgaaaag aaagaaattg tcaggaactt gtacaagatt ttcttttcta tctatttata
    12601 ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggctgtactt gagtacggca
    12661 aggcgacgtt gacgcgattt gaatttgatt ttcgaagagt attattcgta aaaaatctca
    12721 aaaagcctac ctttcggtag acttagcttg tttctattct aatcggcact cttccaaaat
    12781 tttgctctgc tatacttggc tttcctagtt ggtaaatctg gtctgctttt tgggtcatag
    12841 catcccaagg ttctttgcca attcggctga ctagattgac ctgtcctttc agagacttga
    12901 gatgttgcct gcctttttca gtaaatccaa ggacatgaat ggcttctggc aagacatttt
    12961 ctctagcctg caccaaaata taggtcaata ggcgtctgac acgcgccttg gtgtaacgtt
    13021 tggtcgcaac cgcctcgacc aactcttcta cagactgggc tgttttaata gcttctttaa
    13081 tgcgcacagc catttcttga ttgacctgat agatggtggt taggtcggga tttgacaaga
    13141 tttgatagcg gagcaaggga aaatagtctt cccagatcac cttactggcc tgctcaaata
    13201 gggcaacaga aggcataaag cgttctaaga aatcttggtc cttctgatgt tgacggaggg
    13261 ctgtcgccga ggcaaagtcc acatctttat tcacagaatg gtaacctgcc ccctgacgct
    13321 gaataggatg cagcttgata tttcgtcctg caactgcctt ggcataggcc agagcaagaa
    13381 catgattggg ggtattacct gaaaaatcaa gaccagcaaa ttccttccat atagcttggg
    13441 ttttctgggg ataggaaagg gaatcaggca gattttccac aaatttctcc atctcagcac
    13501 ctttctctgt gtataagtca gcgatttttt gataatctag aacttcttct gtcccaaaag
    13561 cgaggctatc tatgcccaac cgagctaaga tatccacggc cccttgaccg aaaaaatctg
    13621 ccgcctgaac gctgactaaa aagggcaatt ccactaccag atccgctcca ttttccagcg
    13681 ccatctgggc tcgtgtccac ttatccacga tagcaggttc tccacgctgc atgaaattcc
    13741 ctgacatagc cacgattttc agcccctccg cttgatccag caggtatttg tgtccattat
    13801 gaaaaggatt gaactccgcg ataatacccg tgatggtcat agtaatctcc tacttcttta
    13861 gtatcaaatt gagtcaatta tattctgtag gctccgtttt tgagtagttg tttcctcaat
    13921 gaatttaatt gaccattctt ttaagtaatt gtgtctacta aaataccagc catttatcca
    13981 cttatttatt aaagtttttt catcatcgtt caacatgtta taatattttc tttc
//
LOCUS       DAVKGU010000048        14028 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKGU010000048 DAVKGU010000000
VERSION     DAVKGU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.048"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(65..262)
                     /locus_tag="VW077_001321"
     CDS             complement(65..262)
                     /locus_tag="VW077_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9455333.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(337..1113)
                     /locus_tag="VW077_001322"
     CDS             complement(337..1113)
                     /locus_tag="VW077_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9455334.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            1235..1981
                     /locus_tag="VW077_001323"
     CDS             1235..1981
                     /locus_tag="VW077_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9455335.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     RQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1994..2974
                     /locus_tag="VW077_001324"
     CDS             1994..2974
                     /locus_tag="VW077_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9455336.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3169..3489
                     /locus_tag="VW077_001325"
     CDS             3169..3489
                     /locus_tag="VW077_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9455337.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3535..3843
                     /locus_tag="VW077_001326"
     CDS             3535..3843
                     /locus_tag="VW077_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9455338.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3860..5158
                     /locus_tag="VW077_001327"
     CDS             3860..5158
                     /locus_tag="VW077_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9455339.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSWYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            5309..7747
                     /locus_tag="VW077_001328"
     CDS             5309..7747
                     /locus_tag="VW077_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9455340.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIGGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7766..8434
                     /locus_tag="VW077_001329"
     CDS             7766..8434
                     /locus_tag="VW077_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9455341.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            8452..9540
                     /locus_tag="VW077_001330"
     CDS             8452..9540
                     /locus_tag="VW077_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9455342.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            9995..12496
                     /locus_tag="VW077_001331"
     CDS             9995..12496
                     /locus_tag="VW077_001331"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9455343.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTS
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEIVAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFVQLIAPFAPHL
                     AEELWQTVAATGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKVEIDGKEIVKVIAVPNKLVNIVVK"
     gene            12691..13386
                     /locus_tag="VW077_001332"
     CDS             12691..13386
                     /locus_tag="VW077_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455344.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQESVADMEELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPKLRGTRIGTEAQYLLARYVFEELNYRRYEWKCDSL
                     NLPSRRAAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKARLEAWLTPENF
                     DKTGQQYKSLREL"
     gene            13398..13814
                     /locus_tag="VW077_001333"
     CDS             13398..13814
                     /locus_tag="VW077_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455345.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YVALDGDAVVGLIRLVGDGFSSVFVQDLIVLPNYQRQGIGSLLMKEALKDYKDAYQVQ
                     LVTEETEKNVRFYRSMGFEALSTYDCIGMTWVNREK"
BASE COUNT         4514 a         2184 c         2940 g         4390 t
ORIGIN      
        1 gatgttcaca tcttatttca attcactata ttagagtaaa attctctaca aaaagaagaa
       61 tagcctattt gactattctt ccgagtgatt tcaatttctt tggggaaata tggagatact
      121 ttttaaatac tttggcaaaa tatttatagt ctgaaaatcc aacttggtca gagatagcgc
      181 ttaaagggga gttgggagat tcagccatct tatctatagc ttgtttcagg cgatattgta
      241 agatatattc atttagagtc atggggaagt cttctttgat aaccttatat agatgcttgt
      301 tatctgcaaa tagctagagc atttcaagga aatgaactag aaataacaat taatatggtg
      361 gttcagaaat accttttgat aatcaataag atcttcagga tgaagtgcgt tgactccatc
      421 catttcatat ttccgattta ataaacgata tttgttttca ccaaattgat gaaaagggag
      481 tagttgaact tggtcgatat ttaatgagtt aaatagagta gcgaattttt ctgcatcctc
      541 taaactattg ttaaaattag gaataactgg gattcttaaa acgatagttt tattttgaga
      601 aaaagcataa tgaatgtttt taataatcat ttgattaaaa accccagtca cttttttatg
      661 ttttatagaa ttataatgtt ttaggtctgt gtagataaaa tccacatatt gaattaaatc
      721 aataaatttt tcatgatcaa caaaggcagt agtttcaatg gcagtgtgta tgtgatgttc
      781 tttagctgat tttaagatgg ctttagcaaa ttcaaactga gcaaatattt cacctcctga
      841 taaagttaaa cctccaccgg attcttcgta aaattctttg tcttttaata cctctgtaat
      901 aatttcttct acagtctttt cttcaccgac taaggtgaat ttctctcgtt gagcatcttt
      961 cattttttca ggtttcattc tttgagattc aggattagaa caccatggac agcgcagagg
     1021 acatcctttt aaaaaaacag ttgtacgaat acccgggccg tcatgaattg aaaagtgttg
     1081 aatattaaaa ataattcctt tagatatttc cataagagtt cctcctcttc ataagtctat
     1141 cctatcacaa aaacgaaaga aaaacaatta cgaacgaaag tttaatttgt tgttttttgc
     1201 tttttatttt ggtagaataa aacggaggtg taaaatgaaa agattggaac aaattattaa
     1261 attagtatca gaatatgaaa agatcgacgt taatacatta tcggaaaaat taaatgtatc
     1321 gaaagtaacg attagaaaag atttagataa attagagtca aaaggtttat tacacagaga
     1381 gcatggatat gctgtattaa atagtggaga tgacttaaat gtacgtttgt caattaatta
     1441 tgaaattaag agaaaaattg ttcaggaagc agtaaaattg gtgtcagata atgaaacaat
     1501 aatgatagaa tctggatcga cctgtgcttt acttgctgag gaaatttgca agcaaaaaag
     1561 aaatgttacg attgtaacaa attcattttt tatagcaaat tttgtgagag cttatgattc
     1621 atgtcgtatt attgttcttg gtggagagtt tcagaaggat tcacaggtta ctgtgggacc
     1681 tttattaaaa gaaatgatac agacttttca tgtgcgtcaa gcttttgttg ggacagatgg
     1741 ctacgataaa gagatgggct ttacaggaaa agatttaatg cgcagtgagg tagttcaata
     1801 tatttcagca gcgtcggata aagtcattgt actaactgac tcaagtaaat ttgataaaag
     1861 gggtacagta agaagatttg ctttaagtca agtctatgaa gtaataacag acgaaaaact
     1921 ttctaaacaa aatatagcta cattagaaaa tgctgggata atggttaagg tagtttcgta
     1981 agaggttaag tgtatgaatc aagataggaa taaactgctt tctaaaattg cttatctgta
     2041 ttatattgaa aacttaaatc agtcacaaat agcagcaaaa ttaggaattt atagaacctc
     2101 tattagtaga atgttaacag aagcaaggaa tgcaggaatt gttaaaattg aaatagagaa
     2161 ttttgataca aatatgttta agttggaaaa ttatgtaaaa gaaaaatatg gtctggaaag
     2221 tttagaaatt attccaaatg aatttgatga taatccaaca attttatctg aaagaatttc
     2281 tcaagttgca gcaggtgtcc ttaggaattt aattgatgat aatatgaaaa ttggcttttc
     2341 ttgggggaaa agtttaagta atttagtaga tttaattcac agtaaaagtg tccgaaatgt
     2401 tcacttctat cctctagcag gtggtcctag tcacatacac gctaaatacc atgtgaatac
     2461 actgatttat gaaatgtcta gaaaatttca tggagagtgt acatttatga atgcaacgat
     2521 tgtgcaagaa aataaattgt tagcagatgg tattttgcaa tcaagatatt ttgaaaattt
     2581 gaaaaatagt tggaaagatt tagatatagc tgtagtcgga attggtgatt ttagcaataa
     2641 aggaaaacat caatggttag acatgcttac agaggatgac tttaaagaat tgaccaaagt
     2701 taaaactgta ggagaaattt gttgtcgatt ttttgattca aaaggtaaag aagtctatga
     2761 aaacttacag gaaagaacga tagcaatctc tttggaagat ttaaaaaata ttcctcaaag
     2821 tttagctgtt gcttacggtg atacgaaagt atcttcgatt ctttctgtct tgcgtgctaa
     2881 tttagtaaat catttgatta cagacaaaaa tacaatttta aaagttttgg aagaagatgg
     2941 ggatttgact tttagagaga ttctaggtga gtgaaaatga tagactgatc agtttatcgt
     3001 tttttctttt tagtcgattg cacatttgtg ctcatataaa caaaaatagt ttatctgttg
     3061 tttttggatt gacaacttta ttatgtagtt gtattctata gttacaaaag aaaattttaa
     3121 aatttcaaat gaaaaaagct ttttacatag tgaaatgagg aggaatttat ggaaattatt
     3181 gttccagatc aaattatcat gggtttaatt ttatatgctg gtgatgcgaa acaacatatt
     3241 tataaagcgt tagattacat aaaaaatggt acatgtgaac ggtgtgaaga agaaatccag
     3301 ttagctgatg ccgccttatt agaagcccat aatctacaaa caaaattctt ggcacaagaa
     3361 gcgtctggta caaagacaga aattacagct ctctttgttc attcacaaga tcatctcatg
     3421 actagtatga cggagattaa tttaatcaaa gaaattatta gtttgagaaa agaacttcat
     3481 aaaaaataat actagagtat tatcattgtt attaacatag aggaggaaaa cataatggtg
     3541 aagattggtt tgttttgtgc agcaggtttt tctactggta tgcttgtaaa taatatgaaa
     3601 attgcagcgc aagctagtgg agttgaggca gaaatagagg cgttttctca gtctaaatta
     3661 gcggattatg cgccaaatat agatgttgca ctattgggtc cacaagttgc ttatacatta
     3721 gataaatcaa aagaaatttg tgataagtgt gatgttccga tagctgttat tccgatgatg
     3781 gactatggta tgttagatgg aaaaaaagta ttagatttgg ccctatcttt gattagtggg
     3841 taagaaaagg agatttatta tggcaaagat ggatgttcag aaaatcattg caccgatgat
     3901 gaagtttgtg aatatgcgtg gcattatagc tctaaaagat gggatgttag caattttgcc
     3961 attgacagta gttggtagtt tattcttgat tatgggacaa ttgccgttcg aaggattaaa
     4021 taagagcatt gctagtgttt ttggagctaa ttggacagag ccgtttatgc aagtatattc
     4081 aggaactttt gctattatgg gtctaatttc ttgtttttca attgcctatt cttatgctaa
     4141 gaatagcgga gtagaggctt taccagctgg agttctatct gtatctgcat tctttatttt
     4201 gctaagatca tcttatatcc ctaaacaagg tgaggcaatt ggggatgcta ttagtaaagt
     4261 ttggtttgga ggtcaaggaa ttatcggtgc tatcattata ggtttggtag taggaagtat
     4321 ttataccttc tttataaaga gaaaaattgt tattaagatg ccagaacaag ttccacaagc
     4381 tattgccaaa cagtttgaag caatgattcc agcatttgta attttcttac tttctatgat
     4441 tgtatatatt ttagcgaagt cattgactaa tggcggaaca ttcatagaaa tgatttattc
     4501 tgctattcaa gttccattgc aaggtttaac tggatcttgg tatggtgcaa ttggaattgc
     4561 attctttata tcatttttgt ggtggtttgg tgttcatgga caatcggtag taaatggagt
     4621 agtgacagct ctgcttttat ctaatcttga tgctaataaa gctatgttag cctctggtaa
     4681 tctatcatta gaaaatggtg cacatattgt tactcaacaa tttttagatt catttttaat
     4741 tctatcaggt tcagggatta cgtttggtct tgtagttgcc atgctttttg cagcaaaatc
     4801 aaaacaatac caagccttag gaaaagttgc cgcttttcca gcaatattta acgtaaatga
     4861 gccagttgta tttggatttc cgattgtcat gaatccagtt atgtttgtac ctttcattct
     4921 tgttcctgta cttgcagctg tgatagtata tggagctatt gcaacaggtt tcatgcagcc
     4981 attctcaggg gtaacattgc cttggagtac accagctatt ttatcaggat ttttggtggg
     5041 tggatggcaa ggagttatta ctcagctggt gatattagcg atgtctacat tggtttattt
     5101 tccattcttt aaagtacagg atcgtttagc ttaccaaaat gaaatcaaac aatcttagag
     5161 gtatttgtgt gttactgtta aactcacaca tttgtgctaa aaattagaga gttaaaattt
     5221 ttctagttaa aagcttgaaa atttctataa aaatcggtat tatattttcg aaagaaataa
     5281 aaatattttc gaaagaaagg tggttacgat ggtaaataca gaagtagcaa gaacaacaat
     5341 caagacagaa tattttggca gccttactga aaggatgaac aaatatcgag aagatgtttt
     5401 aaataaaaaa ccttatattg atgctgagag agcagttcta gcaacacgcg cctatgaacg
     5461 atacaaggaa caacctaatg tcctaaaacg tgcatatatg ctgaaagaaa ttttggaaaa
     5521 tatgactatc tatattgaag aagaatctat gattggggga aatcaagctt cttccaataa
     5581 agatgctcct atttttccgg aatatacgct agaatttgtt ctcaatgagt tggatctttt
     5641 tgaaaagcgt gatggagatg ttttctatat tacagaagaa acaaaagaac aacttagaag
     5701 tattgctccg ttttgggaaa ataataattt acgtgctaga gctggtgcct tattacctga
     5761 agaagtgtct gtttatatgg aaacaggatt cttcggtatg gaaggtaaga tgaattctgg
     5821 agatgctcac ctagcagtta actatcagaa acttttgcaa tttggcttaa gaggttttga
     5881 agagcgggct cgtaaagcaa aagcagctct agatttaaca gatccagcaa gtattgataa
     5941 atatcatttt tacgactcta tatttatcgt aatcgatgct attaaagtat atgcaaagcg
     6001 ctttgttgct cttgctaaaa gtttagccga aaatgcaaat cctaaacgta agaaagaatt
     6061 acttgagatt gcagatattt gctctagagt cccatatgaa ccggcaacta cttttgcaga
     6121 agctattcaa tcagtttggt ttattcaatg tattttacaa attgaatcta atggccactc
     6181 tctttcatat ggccgttttg atcaatatat gtatccatat atgaaggctg atttagaaag
     6241 tggtaaagaa acagaagata gcattgttga acgtctgaca aatctttgga ttaagacaat
     6301 tacaattaat aaggttcgca gtcaatcaca tacattttct tcagcaggaa gtcctttata
     6361 tcaaaatgtt acaattggtg gacagactcg agataagaag gatgctgtta acccattatc
     6421 ttatttggta ttaaaatcag ttgcacaaac ccatctaccg caacctaatc taactgtacg
     6481 ttaccatgca ggtttagatg ctcgtttcat gaatgagtgt attgaagtga tgaaacttgg
     6541 ttttggtatg cctgcattta ataatgatga gattattatt ccttctttta ttgcaaaagg
     6601 agtattggaa gatgatgctt atgattacag tgccattgga tgtgttgaaa cggcagttcc
     6661 agggaaatgg ggctatcgtt gcacaggtat gagttatatg aacttcccta aggttctact
     6721 tatcacgatg aatgatggaa ttgatccggc ttcgggtaaa cggtttgcac caagctttgg
     6781 tcattttaag gatatgaaga acttttctga attagaaaat gcttgggata aaacactaag
     6841 atatttgaca cgaatgagtg ttattgttga aaattctatt gatttatcat tggaacgaga
     6901 agttcctgat attctatgtt cagcattgac tgatgattgt attggtcgtg gaaaacacct
     6961 taaagaaggt ggagcagtat atgattatat atcaggattg caagttggaa ttgcaaattt
     7021 gtcggattca ttagctgcaa ttaaaaaatt ggtgtttgag gaagaacgta taagcccaag
     7081 tcagctttgg catgcactgg aaacagatta tgccggagaa gaaggtaagg tcattcaaga
     7141 aatgttgatt catgatgcac ctaagtatgg taatgatgat gattatgctg acaaattggt
     7201 tactgctgct tatgacattt atgttgatga aattgctaaa tatcctaata cacgttatgg
     7261 aagaggtcca attggaggaa ttcgttattc aggaacatct tctatctcag ccaacgtagg
     7321 gcagggacgt ggaacattag caactccaga tggacgcaac gcgggtacac cgttagcaga
     7381 gggttgttca ccatcacata atatggatca acacggccct acatctgttt taaaatctgt
     7441 ttcaaaatta ccaacagatg aaatcgtagg tggggttctc ttaaatcaga aagtaaatcc
     7501 tcaaacgtta gccaaagaag aagataaatt aaaactaatt gctttgttac gaacattctt
     7561 taatcgttta catgggtacc atattcaata caatgttgtt tccagagaga cgctgattga
     7621 cgctcagaaa catcctgaaa aacacagaga cttaattgtt cgtgttgcag gatactctgc
     7681 attcttcaat gttctttcta aggcaaccca agatgacatt ataggacgta ctgagcatac
     7741 tttgtaaaat aaagaggttc tttttatgga atttatgctt gacacattaa atttagatga
     7801 gattaaaaag tggtctgaaa ttttgccgct agctggggta acttcaaatc ccactattgc
     7861 aaaaagagag ggttctatta atttttttga acgaatcaaa gatgtaagag aattgattgg
     7921 ctctacaccc tctattcatg ttcaggtgat ttctcaagat tttgaaggca tcttaaagga
     7981 tgctcataaa attcgaagac aagcaggaga tgatatattt atcaaagtac ctgttactcc
     8041 agctggatta cgtgcaataa aggcgctaaa aaaagagggc taccatatca ctgcaacagc
     8101 tatttataca gttattcagg gattattagc tatcgaagca ggagcggatt acctagctcc
     8161 atattataat agaatggaaa atctgaacat tgattcaaat tctgtcattc gtcaattagc
     8221 tcttgctatt gatagacaga actctcctag taagatttta gctgcatcct ttaaaaatgt
     8281 agcacaagta aataatgctt tagctgcagg tgcgcatgct gttacagcag gagcggatgt
     8341 ttttgaatca gctttcgcca tgccatctat ccaaaaggcg gttgatgatt tttctgacga
     8401 ttggtttgtt attcaaaata gtcgttccat ttagatagag aggaaataca tatgagaatt
     8461 tttgctagtc cttctagata tattcagggg gaaaatgcct tgtttgaaaa tgccaaatca
     8521 attttggatt tgggaaatta ccctattcta ttatgcgatc agttggttta tgatattgtt
     8581 ggaaaacgat ttgaagatta cctacatagg tatggtttcc atattgttct ggcgctattt
     8641 aatggtgaag cttctgacaa tgaaatcaat cgagttgttg ccttggctga gaaagaaaat
     8701 tgtgatagta ttatcggtct tggtggggga aagacgattg atagcgcaaa agctattgca
     8761 gatttgattg aaaagcctgt tattattgct ccaacaattg catcgaccga cgcacctgta
     8821 tctgctttat ctgttattta tacagatgaa ggtgcatttg atcattatct attttattct
     8881 aaaaatccag atttagtttt ggttgataca aaagttattt cacaagcccc taagcgttta
     8941 ttagcgtctg gtattgcaga tggtttagca acttgggttg aggcgcgtgc ggttatgcag
     9001 gcaaatggaa aaactatgtt gggacaacag caaacattgg ctggagttgc aattgcgaag
     9061 aaatgtgaag aaacgctgtt tgcagatggt ttacaggcta tggcagcttg tgaagctaaa
     9121 gtggtgacac cagcattaga aaatattgtt gaagctaata ctttattgag tggtctaggt
     9181 tttgaaagtg gaggattagc tgcggcgcat gcaattcata atggttttac tgcattgaca
     9241 ggtgacattc atcatttaac acatggtgaa aaagtagctt atggaacttt agtacaacta
     9301 ttattggaaa atagacctaa agaagaactt gataagtata ttgagtttta caaaaaaatt
     9361 ggtatgccaa caactctaaa agaaatgcat ttggatcaag ttggatatga tgatttaata
     9421 aaagttggta aacaagcaac tatggagggt gagacaattc atcagatgcc gtttaagatt
     9481 tcgccttcag atgttgctca agctattatc gctgtagatg cctatgtaaa ttcaaaataa
     9541 acaataagga ctactgtttt ccaaatggta gtcttttatt gatccctgta ttgaattcta
     9601 tagcagattg aaataggatg agaacaaatc gattgggaaa gtaaaattaa tttctataaa
     9661 tgttttagca attgtttcgt actatttcag attcagtcca ctatacgttc ttcataaatc
     9721 aaaaagcgac ataggttgtc ggctatttat tgtgaataca ttaattagca ttccagtttt
     9781 atcttcggtc taaaataagt attttgtgct atacgagata agcttcttga cttactcctt
     9841 gatttactgc ataacaatgg gataaaaagt gggagataga gcaattcata gtcatcaaaa
     9901 ttaatgagat acagtataca gttttttctt taaacacatt tcaaattccc tcaaaaatgg
     9961 tataatagta acatcacaaa attggagaga gaccatgagt ttttacaatc ataaagaaat
    10021 tgaacctaag tggcagggct actgggcaga acatcataca tttaagacag gaacagatgc
    10081 atcaaaacct aagttttatg cgcttgatat gttcccttat ccgtctggag ctggtctgca
    10141 tgtaggacac ccagaaggtt atactgcaac cgatatcctc agtcgttaca aacgtgcgca
    10201 aggctacaat gtccttcacc caatgggttg ggatgctttt ggtttacctg cagagcaata
    10261 cgctatggat actggtaatg acccagcaga atttacatcg gaaaacattg ccaacttcaa
    10321 acgtcaaatc aatgcgcttg gattttctta tgactgggat cgtgaagtca acacaacaga
    10381 tccaaactac tacaagtgga ctcaatggat tttcaccaag ctttacgaaa aaggcttggc
    10441 ctatgaagct gaagtgccag taaactgggt tgaggaattg ggaactgcca ttgccaatga
    10501 agaagtgctt cctgacggaa cttctgaacg tggaggctat ccagttgtcc gcaaaccaat
    10561 gcgccaatgg atgctcaaaa tcacggctta cgcagagcgc ttgctcaatg acttagatga
    10621 actagattgg tcagagtcta tcaaggatat gcaacgcaac tggattggta aatcaactgg
    10681 tgccaatgta actttcaaag taaaaggaac agacaaggaa tttacagtct ttactactcg
    10741 tccggacaca cttttcggtg cgactttcac tgttttggct cctgaacatg aattagtaga
    10801 tgccatcaca agcacagagc aagcagaagc agtagcagac tacaaacacc aagccagcct
    10861 taagtcagac ttggctcgta ccgaccttgc caaagaaaaa actggtgtat ggactggtgc
    10921 ttatgccatc aatcctgtca atggtaagga aattccaatc tggattgcgg attacgttct
    10981 tgctagctat ggtacaggtg cggtaatggc tgtgcctgcc cacgatcaac gtgactggga
    11041 atttgccaaa caatttgacc ttccaatcgt cgaagtactt gaaggtggaa atgttgaaga
    11101 agctgcctac acagaagatg gccttcacgt taattcagac ttcctagatg gactcaacaa
    11161 agaagacgct attgccaaga ttgtggcttg gttggaagaa aaaggctgtg gacaagagaa
    11221 ggtaacctac cgtctccgcg actggctctt tagccgtcaa cgttactggg gtgagccaat
    11281 cccaatcatt cattgggaag atggaacttc aacagctgtc cctgaaagtg aattgccact
    11341 tgtcttgcct gtaaccaagg atatccgtcc ctcaggtact ggtgaaagtc cactagctaa
    11401 cttgacagac tggcttgaag tgactcgtga agatggtgtc aaaggtcgtc gtgaaacaaa
    11461 tactatgcca caatgggcag gttcaagctg gtactacctc cgctatattg acccacacaa
    11521 tactgagaaa ttggctgacg aggatctgct caaacaatgg ttgccagtag atatctacgt
    11581 gggtggtgcg gaacatgctg tacttcactt gctttacgct cgtttctggc ataaattcct
    11641 ctatgacctc ggtgttgttc caactaagga accattccaa aaactcttta accaaggaat
    11701 gattttggga acaagctatc gtgaccaccg tggggctctt gtggcgactg acaaggttga
    11761 aaaacgtgat ggttctttct tccatgtgga aacaggggaa gagttggagc aagcgccagc
    11821 caagatgtct aaatcgctca agaacgttgt taacccagac gatgtggtgg aacaatacgg
    11881 tgccgacact cttcgtgttt atgaaatgtt tatgggacca cttgatgctt cgattgcttg
    11941 gtcagaagaa ggtctagaag gaagccgtaa attccttgac cgtgtttacc gtttgattac
    12001 aagcaaggaa attgttgcgg aaaacaatgg cgctctcgac aaagtttaca acgaaaccgt
    12061 taaagctgtc acagaacaaa ttgagtccct caaattcaac acagccattg ctcaacttat
    12121 ggtctttgtc aacgctgcca acaaggaaga caaactttat gtagactacg ccaaaggctt
    12181 cgttcagttg attgctccat ttgctcctca cttggcagaa gagctctggc aaacagttgc
    12241 agcaacagga gaatctatct cttatgtagc ttggccaact tgggatgaaa gcaaattagt
    12301 tgaagacgaa atcgaaatcg tcgtccaaat caaaggtaaa gtccgtgcca aactcatggt
    12361 tgctaaagac ctgtcacgcg aagaattgca agaaattgct ctcgctgatg aaaaagtcaa
    12421 ggtagaaatt gacggtaagg aaatcgtgaa agtgattgcg gtacctaaca aactcgttaa
    12481 tatcgtcgtt aaataacgag tttattagct ctatctgcca ccttcaatag tccactggac
    12541 tattgaagcc aactaaattg gttaatattg ttgtgaaata agataggaat ccttcagagt
    12601 agaatctggg ggattttttg aatcttctta tgaaagtatg ttatactatg gtcaactata
    12661 aaatttgaaa agcgaaacaa ggggaaagtt atgccagtaa atgaatatgg tcagatgatt
    12721 ggtgagtcaa tggaaggtta tacacctggt gcactgcctt ctattgattt cttagaaggg
    12781 cgttatgctc gaatagaagc tctttcagtg gaaaagcatg cggaggattt attagctgtt
    12841 tatggccctg atacgcctcg ggaaatgtgg acctacctct ttcaggaatc agtggcagat
    12901 atggaggagc tggttagcct tttaaatcag atgttggctc gtaaggaccg tttttactat
    12961 gcaatcatag acaaggcaac tggtaaggct ttgggaactt tttctctcat gcgtattgac
    13021 cagaataatc gagtaataga agtgggagct gtcacttttt ctccaaaact cagggggaca
    13081 cggataggga cagaagccca gtatctactg gcgcgctatg tctttgaaga gcttaactat
    13141 cgtcgctatg agtggaaatg tgattctctt aatctgccat ccagacgagc agcggaacgt
    13201 ttgggatttg tctatgaagg aaccttccgt caggcagtgg tttataaggg gcgtacgagg
    13261 gatacggatt ggttgtctat gattgataag gactggcctc aagtcaaagc tcgattggaa
    13321 gcatggctga ctcctgaaaa ttttgataaa actgggcaac aatacaagag tttgagagaa
    13381 ctttaagagg tgttgagatg attactatta aaaagcaaga aattgtcaag ctagaggatg
    13441 ttttgcatct ctatcaggct gtcggttgga caaattatac ccatcaacca gagatgctgg
    13501 agcaggcctt atctcattca ttagtgattt atgtggcgct tgatggtgat gctgtagtgg
    13561 gcttgattcg tttggttgga gatggttttt catcagtttt tgtacaggat ttgattgttt
    13621 tgcctaacta tcagcgccaa gggattggta gtctcttgat gaaagaggct ttaaaagatt
    13681 acaaagatgc ctatcaagtc cagctggtga cagaagagac agaaaaaaac gtaagatttt
    13741 atcgttctat gggttttgaa gccttatcca cctatgattg tataggaatg acttgggtga
    13801 atcgagaaaa ataacaaaac ttgttttttc ttaagcaaag tttaaggatg gtctagtatc
    13861 atatagtcat taaataaaga cctcctaact ttatttaata aaatcctaaa cttttttcat
    13921 cacaatctcc taatgaagcc acccaatcag gtggcttttt tgcgtggtga ggtgctggtg
    13981 atagaaattt tttgcaaaat agtaaaattt tagaaaagtt aagctagt
//
LOCUS       DAVKGU010000049        13988 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKGU010000049 DAVKGU010000000
VERSION     DAVKGU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.049"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            82..879
                     /locus_tag="VW077_001334"
     CDS             82..879
                     /locus_tag="VW077_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9455346.1"
                     /translation="MISSEFISKIEFACNKKEGLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1027..2287
                     /locus_tag="VW077_001335"
                     /pseudo
     CDS             1027..2287
                     /locus_tag="VW077_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2383..3657
                     /locus_tag="VW077_001336"
     CDS             2383..3657
                     /locus_tag="VW077_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9455347.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKEHDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VW077_001337"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VW077_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9455348.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="VW077_001338"
     CDS             4786..5088
                     /locus_tag="VW077_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9455349.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="VW077_001339"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="VW077_001339"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9455350.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /locus_tag="VW077_001340"
     CDS             5962..7302
                     /locus_tag="VW077_001340"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9455351.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLIIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="VW077_001341"
     CDS             7280..7492
                     /locus_tag="VW077_001341"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9455352.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="VW077_001342"
     CDS             7489..8364
                     /locus_tag="VW077_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9455353.1"
                     /translation="MKKQEKLALVESVLEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIASLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIVFCNQTLDQANEKLEEIAQQVPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="VW077_001343"
     CDS             8357..9172
                     /locus_tag="VW077_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9455354.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="VW077_001344"
     CDS             9165..9596
                     /locus_tag="VW077_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9455355.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="VW077_001345"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="VW077_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9455356.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSEIYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="VW077_001346"
     CDS             11272..12000
                     /locus_tag="VW077_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9455357.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="VW077_001347"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="VW077_001347"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9455358.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4139 a         2544 c         3344 g         3961 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaagattt aggagtttat tatgatttct tcagaattta tctcaaagat tgaatttgct
      121 tgcaataaga aagaaggtct ttatagtcaa agcaaattta agtatgcgat tcgttcgatg
      181 ttcgcaggtg catttttaac cttcagtact gctgcaggtg cagttggggc tgacttgatt
      241 aataaaattg caccaggtag tggacgcttc ctctttccat tcgtttttgc ttggggcttg
      301 gcctacattg tttttttgaa tgccgagttg gtcacttcaa acatgatgtt cttgactgct
      361 ggtagtttct taaaaaaaat ctcttggaga aaaacagctg agattttact atactgtacc
      421 ttgttcaacc ttatcggagc cttgatagca gggtggggct ttgctcattc ggcagcctat
      481 gcgaatctga cacacgatag tttcatctca ggtgttgttg agatgaagtt aggccgctca
      541 aatgaattgg tcttgcttga ggcgattttg gcaaatattt tcgtaaatat tgcgattctg
      601 tcatttattt tggtcaaaga tggtggtgcc aaactttggc ttgtgttgtc agctatttac
      661 atgtttgtat tcttaacaaa cgagcacatt gcggcgaact ttgcttcttt cgcgattgtg
      721 aaattcagtg ttgctgcgga ttcaattgcc aacttcggtg ttggaaatat gcttcgccac
      781 tggggtgtga ctttcatcgg aaactttatc ggaggaggcc tcttgatggg tcttccatat
      841 gccttcctca ataaaaacga agatacttat gtagattaag aaaatgagca cgattgagtc
      901 gtgctttttt cattttcaaa ataaggtaac agctatttct tatatcaaaa tatagaaaac
      961 tgatatttgt aaactataac tcaaggtgct acaatatcct taataaaata atatggaggt
     1021 caccttatga cttgtgattt taaatttgaa actctacaac tacatgctgg tcaagttgtg
     1081 gctccagcta ctaagtctcg tgcagtgccg atttaatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atatgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgcttgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttg tcgatattga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaaca ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgcgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggacggctt cagggaaatt ccctcaattt gttgacgagg gtccaagctg
     1741 ccacaatttg agctatactc gtgatgtggg tgcagcagcc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa accctttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaagataaat tatccaaaac ttgcagatag
     1981 tccttatcat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccatgtcaaa ggtggcgagg aagaagcacg caaggtcatt gataatttgg aaatcttctc
     2101 agaccttgct aacgtggcag atgccaagtc tcttgttgtt catccagcaa caaccactca
     2161 tggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaatc aaatccactt
     2221 gtctatcgga cttgaaaatg tagatgattt gattgaagac ttgcgcttgg ccttggaaaa
     2281 aatttaaaga aaaagaagat aaacagtggt tttcgactcg ctgtttttga ttttccttca
     2341 tgcatgatat aatggttaca gaagtctaga aagaggaatg taatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gaaagccagt atgctgaact tttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaggcagga actggccttg
     2521 gctgagcaaa aggctatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattac agagtcagat ccaaaacttt gatacagaaa aagaattggc caagaaagaa
     2641 gttgaacaga caagccatca ggccttattg gctaaggaca aggaagtaca ggctttggag
     2701 aaccaattgg ccactttgcg tttggagcat gaaaatcaac tgcaaaagac cctttctgac
     2761 ctagaaaaag aacatgatca ggtcaaaaat cagctcctct tgcaagaaaa ggaaaatgag
     2821 ttgtctttgg cttctgttaa gcaaaactat gaagcccagc ttaaagcagc tagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggccgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagccacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc agttgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgacc
     3541 acatctgaaa accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat caacttaaaa aaagaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaattactc tgggttattc cacgacgact
     3901 gaggatgcta gtggggaagt gatcgcagaa acccctgttt tgtctccctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta cccagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac ttacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctggt gggaacttag gcccaggatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata ccaaacggtc
     4921 atggccaatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggagcctcaa gatgtcttta
     5521 tcgttgaggg cattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgta ttattcgtcg tatcaagcgt gatatggagg
     5641 aacgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat aaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaaatat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtctatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga aggtctgttt caagaacgct tcaagcaagc tctaccccaa
     6361 ttttctaaga gaattggggt ggtaaccagc cgtagtggag ccgttattcg agatattatc
     6421 acaaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatccgac caaggttcaa
     6481 ggtgaagggg ctgcggagga aattgctaga aatattgcgc gtgctaatca acgggacgat
     6541 ttggatttga tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc tagtgtgggg
     6661 catgagacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattagctca tttgcaaaat
     6781 caggaaaaac ggatggcaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccattgta
     7201 gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggta aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gtcggttttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttgcca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttggagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagacccat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagatcaa ggtggacttg attgccagct tatcccttgc ttcaggtagt
     7921 ctgggtatgg tggcagggca agttttggat atggagggcg aacaccagca cttgtctctg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcccttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gtaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ttgttgggct tggaagagtc cattgtcttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattggaag aaattgccca gcaggttccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgacaccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcaattcaa
     8761 ttttcgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta agattcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggca ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaaa
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattgccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgagacttt tgcagacaag atttttagct gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgtctttta caggaaattt
     9841 ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gcacgtatcg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct ctctgtctga gatctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctca
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtgggactg
    10561 ttgatgatgt tttgctttat tttgccaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc gaagctgaga
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaatgaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt cataaaattg
    11041 gtcagcatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattctt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtctct
    11161 tgacggtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag
    11221 aagcagccct gacgcaagcc agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tcttaaaacc
    11341 tgggatggtc agacccagtt gctctttcta ggtgatttga ttgacagagg tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtct
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatcgtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcggcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatctttgt tcacgcaggt
    11701 attgatttga ccttggacga ctggcatgaa acaacagatt ataaaaaagt atggcttaga
    11761 aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatggc
    11881 aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat tgtctttaca
    11941 gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gctttgtcgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacagga gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccac ggaaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggag
    12241 ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa ttacactgca
    12301 aaagatgggg aaacttatat tttccacttg attgacacac cagggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt cgatgcggct
    12421 caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa tgatttggaa
    12481 atcatgccta tcattaataa aattgacctg ccggctgcag atccggagcg cgtgcgtaca
    12541 gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc tgccaaggct
    12601 ggtattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc accaacgggt
    12661 gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttatcctcc aagtacgtgt catggacgga gtggtcaaac ctggtgataa gattcagctc
    12781 atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc aaaagcggtt
    12841 ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat caagacggtt
    12901 caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc agaaccatta
    12961 catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgaatcaaac
    13021 aagtacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcacttgga tttggtttcc gttgtggatt tcttggactt
    13141 ctccatatgg atgttatcca ggaacgttta gagcgtgagt tcaatattga cctcatcatg
    13201 acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgtcaag
    13321 gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag
    13381 cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attcctcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc aacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt gaaaatggat
    13561 atccttctca atggagataa ggtggatgct ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt
    13681 gaagttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt tgaagttccg
    13861 caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag aaatgccttt
    13921 ccttgttcac aggaaattta tataagaatc aatagtaaag caggcttaga tggtctgctt
    13981 ttttagtt
//
LOCUS       DAVKGU010000050        13814 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKGU010000050 DAVKGU010000000
VERSION     DAVKGU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.050"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(56..964)
                     /locus_tag="VW077_001348"
     CDS             complement(56..964)
                     /locus_tag="VW077_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9455359.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            1028..1297
                     /locus_tag="VW077_001349"
     CDS             1028..1297
                     /locus_tag="VW077_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9455360.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            1297..1677
                     /locus_tag="VW077_001350"
     CDS             1297..1677
                     /locus_tag="VW077_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9455361.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            1766..1993
                     /locus_tag="VW077_001351"
     CDS             1766..1993
                     /locus_tag="VW077_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455362.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(2033..2758)
                     /locus_tag="VW077_001352"
     CDS             complement(2033..2758)
                     /locus_tag="VW077_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9455363.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            2920..4761
                     /gene="typA"
                     /locus_tag="VW077_001353"
     CDS             2920..4761
                     /gene="typA"
                     /locus_tag="VW077_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9455364.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            4781..5035
                     /locus_tag="VW077_001354"
     CDS             4781..5035
                     /locus_tag="VW077_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9455365.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            5399..5698
                     /locus_tag="VW077_001355"
     CDS             5399..5698
                     /locus_tag="VW077_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9455366.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            5769..7790
                     /locus_tag="VW077_001356"
     CDS             5769..7790
                     /locus_tag="VW077_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9455367.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            7792..8433
                     /locus_tag="VW077_001357"
     CDS             7792..8433
                     /locus_tag="VW077_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455368.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLSLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            8537..9889
                     /locus_tag="VW077_001358"
     CDS             8537..9889
                     /locus_tag="VW077_001358"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9455369.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVAFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            9893..10951
                     /locus_tag="VW077_001359"
     CDS             9893..10951
                     /locus_tag="VW077_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9455370.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGNKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            10961..12127
                     /gene="divIB"
                     /locus_tag="VW077_001360"
     CDS             10961..12127
                     /gene="divIB"
                     /locus_tag="VW077_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9455371.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESN"
     gene            12393..13094
                     /gene="pyrF"
                     /locus_tag="VW077_001361"
     CDS             12393..13094
                     /gene="pyrF"
                     /locus_tag="VW077_001361"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9455372.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPIIQAEDPV
                     AAYHTIKDEWTQDWN"
     gene            13128..13760
                     /locus_tag="VW077_001362"
     CDS             13128..13760
                     /locus_tag="VW077_001362"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9455373.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVDAIKEAFPEVEVIAGTATAGIPHGAIIADKMNLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
BASE COUNT         4184 a         2529 c         3072 g         4029 t
ORIGIN      
        1 aaaaagaacc aatgtgcagg ttgttgctag cctacatatt ggttcttatc atttctcaac
       61 tatcaaactg aacttcttct agtagatagt caataaagcg ttcgcccatc ttagataggc
      121 tggttttctc atgctggata tagaccagct caatcgggtc atcaatatcc agtgggatag
      181 aaacgatatt gtctccgttt aggttgctgt tcaaaatccc tgtcgcaatg gtataaccat
      241 ccaaaccaat caagagatta aagagggtgg cacggtcact gaccacaatg gatttcttgt
      301 ggtgttcttg agaaagaatc tcttctgaaa agtagaagga gttgtgcgtc ccttggtcat
      361 agctgaggta agggaaattc tccaaatcag acagtttcac cttgtctttc tttgccagag
      421 ggttggtctt gctgacaaag atatgcggtt gcgctgtgaa gagatggtgg gctagcaggt
      481 gattgtcatc cagcatcttg gttaaaacat cacggttgta actgtttaag aagaggaccc
      541 cgacctcact gcggaagttc ttgacgtcgt cgataatctc ccaagtccga gtttcacgaa
      601 ggaagagttc gtatttctcc atatcgcttt tcttgagcaa agagacaaag gcattgacca
      661 caaaggcata gtgttgagac gaaacgctaa agagttcgcg gtgggcaaca ggatttttat
      721 agcgttcctc cagaagctgg gtctgctcga caacctgacg ggcataagag agaaactcca
      781 tcccatcacg ggtcaaggta atacccttgg gattgcggat aaagatttca atgcccattt
      841 cattttccaa atctcgcact gcattggaga gactgggctg agtgataaag agttgcttgg
      901 ctgcctcatt catagagcca gtttcgacga ttttgataat ataatgtaat tgttgaattc
      961 tcatgttttt attgtaccac acttgcctta gaatcgccaa agaagatagg aaaataagga
     1021 gcattgtgtg agtctcttct tgaaaaaaac acaaaatttt gctagaatga gtcttatgaa
     1081 aacattctat gatgtgcagc aattcctcaa acgatttggt attattgttt acatgggaaa
     1141 acgcttatat gatattgaac tgatgaagtt ggaactatct cggatttacg atgcagggtt
     1201 gatggataaa ttagactatc tagaggcaga agcagttctt cgcagagagc acaaggtaga
     1261 attggattat attgagaaaa atggagaaaa gaactaatgg ttacttggat tttgtgggca
     1321 cttatactag caatgttggc gtggatgggc tttaactatc ttcgtattcg tcgtgcggct
     1381 aaaattgtgg acaatgagga gtttgaagcc ttgattcgta cgggtcaatt gattgatttg
     1441 cgcgacccag cagaattcca cagaaaacat atccttggtg cacgcaatat tccttcaagt
     1501 cagttgaaaa ctagtcttgc agcccttcgt aaagataaac ctgtccttct ctacgagaac
     1561 caacgtgcgc aacgagttac aaatgcagct ctttacttga aaaaacaagg tttttctgag
     1621 atttatatcc tttcttatgg cttggattct tggaaaggga aagtgaagac tagctaacct
     1681 tatttccaaa ttagtcgaat gataggatga agctattgtt ttatagtata attaatttgt
     1741 ataaaattta aggagttctt agaacataaa agaaaagaag aaaaatccac tttttgtggg
     1801 tatcttgtcg attattcttg gtttgttatt tccaattgta ggcctgattt tgggtatcat
     1861 aggattggtc ttggctattt cataccaaaa agagtcacaa ttagactata aaatagaaaa
     1921 gattcttaat atcttaggaa ttgtgatttc tgtagttaac tggattgtag caatcgcctt
     1981 aatttttcgg taaataaaag aaaaaagctt tagttttaaa gcttttttta ttttatcgac
     2041 tcatctctaa atagttttta atgatttgta actctttttg gttcaaaggg cggtagttac
     2101 cttctgctaa atctgggttc aatgtgaagt ccccaaattg gattctcttg aggctagtta
     2161 ccttaacacc aaccgagagg aacattttct tgatttgatg aaattttcct tctgaaatgg
     2221 tgatagaggc ttgactgagg gaaggacttg cagatagaat ctctagtttt gcgggtttac
     2281 agacagtacc atctaaaaag acaattccct tttgaaaggc ttggatatgg tcaggtgtta
     2341 gaagtccatt aacctcaact tgataagtct tatcgacatg atattgggga tggaggagtt
     2401 ggaagcccaa gggaccgtta tcggtcaaga gaaggagtcc tgttgtatct cggtccagtc
     2461 ggccgacggc atagagcttg tcagactgga tgtcagatgg aagcaggtcc atgacggtcg
     2521 gaagttcctt gtctttgttg gctgtaacga caccagcagg tttatgaagc ataagatagg
     2581 tgtgttcata gccttgaatg attcgatcct gaaaaaggag ttcttgtagt cctgtatcga
     2641 tattttgagc tagggagcgg gctgggcaac catcgactag aatttcccct ctgaggagtg
     2701 cttgcttcat ggcctttcgg ctgatttttt cttgggctaa taaattatct aaacgcatac
     2761 tgtgcctatc ttatcataag tcgcttgctt tgaaaaggct tgacgtgaaa agaaaagcgg
     2821 agtgaaaaca tttacacttg cttgagttat gttatttatt tgaaattatg gtataatcgt
     2881 tcagttagaa aataaatttt gaatattata gaggaaatca tgacaaaatt aagagaagat
     2941 atccgtaaca ttgcgattat cgcccacgtt gaccacggta aaacaaccct ggttgacgaa
     3001 ttattgaaac aatcagaaac gcttgatgca cgtactgaat tggcagagcg tgctatggac
     3061 tcaaacgata tcgaaaaaga gcgtgggatt accatccttg ctaaaaatac tgccgttgct
     3121 tacaacggaa ctcgtatcaa cattatggac acaccaggac acgcggactt tggtggagaa
     3181 gttgagcgta tcatgaaaat ggttgacggt gttgtcttgg tcgtagatgc ctatgaagga
     3241 accatgccac aaactcgttt cgtattgaaa aaagccttgg aacaagacct tgtcccaatc
     3301 gtggttgtta acaaaatcga taagccatca gctcgtccag cagaagtagt ggatgaagtc
     3361 ttggaacttt tcatcgagct tggtgcagat gacgaccagc ttgatttccc agtggtttat
     3421 gcttcagcga tcaacggaac ttcttcattg tcagatgatc cagctgacca agaagcgact
     3481 atggcaccaa tctttgacac gattatcgac catatcccag ctccagtaga taactcagat
     3541 gagcctttgc agttccaagt gtcacttttg gactacaatg acttcgttgg acgtatcggt
     3601 atcggtcgtg tcttccgtgg tacagttaag gttggggacc aagttaccct ttctaaactt
     3661 gacggtacaa ctaaaaactt ccgtgttaca aaactcttcg gtttctttgg tttggaacgt
     3721 cgtgaaatcc aagaagccaa agcgggtgac ttgattgccg tttcaggtat ggaagacatc
     3781 tttgtcggtg aaaccatcac tccgacagat gcagtagaag ctcttccaat cctacacatc
     3841 gatgagccaa ctcttcaaat gactttcttg gtcaacaact caccatttgc tggtaaagaa
     3901 ggtaaatggg taacttctcg taaggtggaa gaacgcttgc aggcagaatt gcaaacagac
     3961 gtttcccttc gtgttgaccc aactgattca ccagataaat ggactgtttc aggacgtgga
     4021 gaattgcact tgtcaatcct tatcgaaaca atgcgtcgtg agggctatga acttcaagta
     4081 tctcgtccag aagttatcgt aaaagaaatc gacggtgtta aatgtgaacc atttgaacgt
     4141 gtacaaatcg acactccaga agaataccaa gggtctgtta tccaaagcct ttctgaacgt
     4201 aaaggtgaaa tgttggatat gatttcaact ggtaatggtc aaactcgttt ggtcttcctt
     4261 gttccagcgc gtggtttgat tggatactca actgagttct tgtcaatgac tcgtggttac
     4321 ggtatcatga accatacctt cgaccaatac ttgccattga ttccagggga aattggtgga
     4381 cgtcaccgtg gtgcccttgt ttctatcgat gctggtaagg ctacaactta ctcaatcatg
     4441 tctatcgaag aacgtggtac gatctttgtc aacccaggta ctgaggttta tgaaggaatg
     4501 atcatcggtg aaaactctcg tgaaaatgac ttgacagtta acatcacgaa ggcaaaacaa
     4561 atgaccaacg ttcgttcagc tactaaggac caaacagctg ttatcaagac gcctcgtatc
     4621 ttgacacttg aagagtctct tgagttcttg aacgacgatg agtacatgga agtaacgcct
     4681 gagtctatcc gtttgcgtaa acaaatcctt aacaaagcag agcgtgagaa agctaacaag
     4741 aagaaaaaat cagctgaata agagctagaa agagagaaag atggtctatt tagtcctagg
     4801 aattttactg ctcctactct atgtatttgc gacaccagaa agcattaaag ggactgtcaa
     4861 tatcgtcgct atggtatgta ttttagtggc actcttgatt ttattggttc tatcttttct
     4921 gaaaattttt caattaccaa cagaaatatt cctagcaata gccatgttga tcctagctta
     4981 ctttagtgtt agagacatca cactcatgcc agtcaaaaaa agtaaaagaa gataaaaaag
     5041 agagtttcgg ctctcttttt gctaggaatt ttacaatagt ttgaattgct ttttagttaa
     5101 caccatctct tttgtttgga taatatgaat gggaaaaact tgaataaaat tctttaatgg
     5161 catctatttt atatttggtt agtactggtt gcatattatt ttgaaataaa actactatat
     5221 aatattattc atagaggcaa tgtagtaaaa tatctaaaaa attcgatttt ttccttgaca
     5281 acaaataata aaaatgatat ttttatcaca taagaaagcg gtttcaacaa aagagagagg
     5341 agggtttatt ttgttgcatt gttgctcaag gtgtgctttt tttgaaagaa aaaataaaat
     5401 gaaaacgaaa aaacatagat tacttgctct agctcttatt tcaagtttta cattattggg
     5461 agctgcatca gctgctgtac aatatccaga tggaggagta tggacgtatg gagaaggttc
     5521 aggaggtggt tgggcttttt caaattacta tcatggtaaa aaatatcatt attcttctct
     5581 tgtaagtaga tggaatagtc attcagataa aggagaagct tctgctggaa aaacctccta
     5641 tgcatggatt tggactaaat ggggagaaca agtagcattt tactgtgatt atgactaatc
     5701 gttcattgat ttaaatcgtt ccccttgtaa gaaattacag ggggaatttt gtttacaagg
     5761 agtctactat gaagaaacta ttcatattat tatcaacttt ttttctcagc ttcttccttg
     5821 cttggattat tgtcttacgt gcgccacaat atttatatgc aagctatgat tccgtttctt
     5881 tacttcgtgt caaaaaagat actcaggaac cgacgcgtga ggtatttgaa caggaattgg
     5941 agaattttgc aaactcagaa cagagtttaa tagctagaag aattgtagag ccgagtaagg
     6001 atgggacgac tcactttact tatgcaactt atggtcaggg aactttacca aaagaattcc
     6061 aagaagctag ccaagaaagt cgtgaacgta gtgatccgct aaatagttat ctccttttgt
     6121 caggctcctt gacgaaagaa aagcttgccg ataaattagg agatttgggt tataaagcaa
     6181 gtgctgaccg aaagataccg ccctattttc ttgcttttcg aatattacta aatcccctta
     6241 ttttaattag tttagcaata tttggcttat ctttctttgc tttagtgatt atcactcgga
     6301 ttaaggaaat gagagcagca ggtataaaac tcttttctgg tcagactctc ttatccatca
     6361 tggggcattc tttatctact gatatcaaat ggctccttct atcagccctc ctttccttcc
     6421 taggtggggg agtcgttctt tttagtcaag gtttgtttta tcctatcttg ttagccacct
     6481 atggttttgg gattagtttc tatctgttgt ttttattggc gatttcaatt ttactaatgc
     6541 ttctttatct aatgagtttg agttacaaag cattagttcc cgttattaag gggagattac
     6601 cccttaaacg cctgatgatt ttaaccctat tgtgtcagtt ggttgctgtc tttacagtag
     6661 gctacgctgt taagacaggt ttgatgtctt accaacgatt gaaagaactt gaaatttcaa
     6721 aacaagcatg gcaggataga gcagactatt atcaaatttc ttttggctta ggtgatagag
     6781 gaaaagatac agaaaatcag agcaagtggt atgcctttgc caaggaagca atcgaagaag
     6841 aacaagctct ttatgtaaag gataatctgc tccattttgc caatccacaa ggaaaaaatg
     6901 aacagggaga gacactggat acctatagtc cagatgctaa tacgctctat gttagtccca
     6961 gttatttgga caaggaaaag gtcgtggtag atgctgagac caaacagaag ttagcccatc
     7021 tccaaaaagg tgagtttatc ctcttgctcc cagaacattt gcgctctcga gaagcagaac
     7081 ttaagaaagt ttttgaagaa agattgagtt attatggaaa atctggtgag gaggcaagtg
     7141 ctcctttgga ttatgagatg aaagcgcacg ttagttatct ttcaatggga gaaaagcggt
     7201 ttgtttataa taacggtgag aatcccgtat ccactcagta tttgactgat ccgattttag
     7261 tggtattcac gccgacttct acaggtgata gttttatatc cttatctagt tggtctatca
     7321 atgctggaaa acaactcttt atcaaaggat atgagagtgg gctagaactc ttgaagaaag
     7381 ctggaattta tgagcaagta tcctatctta aagaaggaag aagtgtttat ctaactcgtt
     7441 ataatgaagt tcaaactgaa acagcaactt taatcttagg agctattgtg gggatagcta
     7501 gttccttgtt actattttat tctgtcaatc ttctatattt cgagcaattc cgccgagata
     7561 tcttgattaa acgaatttca ggtttacgat tttttgaaac acatgctcag tatatggtta
     7621 gccaatttgc cagttttgta tttggtgcta gtctctttat tttaagcagt cgagacttgg
     7681 tgattggctt gctcacttta ttggtctttc tagctagtgc agttttgacg ctttaccgtc
     7741 aagcgcagaa agaatctcgt gtttctatga caattatgaa aggaaaatag gatgattgaa
     7801 ctaaagaata tatctaaaaa atttggaagc cgtcagctat tttcagatac gaatcttcat
     7861 tttgaaggtg ggaaaattta tgccttaatc ggtacaagtg gctgtggtaa gacaacactc
     7921 ttgaatatga ttggacgatt ggagccatat gacaaagggc aaatcatcta tgatggcact
     7981 tctcttaagg acatcaagcc ttctgttttc tttagagatt acttaggata cttatttcaa
     8041 gattttggct taattgaaag ccaaaccgtc aaagagaatc tcaatctgag tttagttggt
     8101 aaaaagttga aggaaaaaga gaaaatctct ttgatgaaac aagctctaaa ctgtgtaaac
     8161 ctctcttatt tggatttgaa gcaacctata tttgagttat caggaggaga agcacaacgt
     8221 gttgcactag cgaagataat tttaaaggat ccgcctttga ttcttgcaga tgaaccaacc
     8281 gcttccttag accccaaaaa ttctgaggaa ttactttcca tcctagaatc tttaaaaaat
     8341 ccgaatcgga ccattattat tgcgacccac aatcctctga tttgggagca agtggatcag
     8401 gtcattcgag ttaccgattt atcacataga tgatatggta agattcagtt agaagaaaga
     8461 gtcacaaaca cactttgtgg cttttttatt tccataaaaa tggtaaaata gtaggagtag
     8521 aaatggagtt cgagacatga aagtaataga tcaatttaaa aataagaaag ttcttgtttt
     8581 aggtttggcc aagtctggtg aatctgcggc tcgtttgttg gacaagttag gtgccattgt
     8641 gacagtaaat gatgggaaac cattcgagga caatccagct gcccaatgtt tgctggaaga
     8701 agggatcaag gtcattacag gtggccatcc tttggaactc ttggatgaag agtttgccct
     8761 tatggtgaaa aatccaggta tcccctacaa caatcccatg attgaaaagg ctttggccaa
     8821 gggaattcca gtcttgactg aggtggaatt ggcttatttg atttcagaag caccgattat
     8881 tggtatcaca ggatcgaacg gtaagacaac cacaacgact atgattgggg aagttttgac
     8941 tgctgccggg caacatggtc ttttatcagg gaatatcggc tatccagcta gtcaagtggc
     9001 ccaaactgcg tcggacaagg acacgcttgt tatggaactt tcttctttcc aactcatggg
     9061 tgttcaagaa tttcatccag agattgcggt tattaccaac ctcatgccaa ctcatatcga
     9121 ctaccatggg tcattttcgg aatatgtagc agccaagtgg aatatccaga acaagatgac
     9181 agcagctgat ttccttgtct tgaactttaa tcaagacttg gcaaaagact tgacttccaa
     9241 gacagaagcc actgttgtag cattttcaac acttgaaaag gttgatggag cttatctgga
     9301 agatggtcaa ctctacttcc gtggtgaagt agtcatggca gcgaatgaaa tcggtgttcc
     9361 aggtagccac aatgtggaaa atgcccttgc gactattgct gtagccaagc ttcgtgatgt
     9421 ggacaatcaa accatcaagg aaactctttc agccttcggt ggtgtcaaac accgtctcca
     9481 gtttgtggat gacatcaagg gtgttaaatt ctataacgac agtaaatcaa ctaatatctt
     9541 ggctactcaa aaagccttgt caggatttga caacagcaag gtcgtcttga ttgcaggtgg
     9601 tttggaccgt ggcaatgagt ttgacgaatt ggtgccagac attactggac tcaagaagat
     9661 ggtcatcctg ggtcaatctg cagaacgtgt caaacgggca gcagacaagg ctggtgtcgc
     9721 ttatgtggag gcgacagata ttgcagatgc gacccgcaag gcctatgagc ttgcgactca
     9781 aggagatgtg gttcttctta gtcctgccaa tgccagctgg gatatgtatg ctaactttga
     9841 agtacgtggc gacctcttta tcgacacagt agcggagtta aaagaataaa atatgaaaaa
     9901 aattgtcttt acaggtgggg ggacggttgg acacgttacc ctcaatcttt tgttaatgcc
     9961 caagttcatc gaagatggtt gggaagtcca ctatatcggg aacaagcgtg gtatcgaaca
    10021 ccaagaaatc cttaagtcag gtttggatgt cactttccac tccattgcga ctgggaaatt
    10081 gcgtcgctat ttctcttggc aaaatatgct ggacgtcttc aaagttggct ggggaatcgt
    10141 ccaatcgctc tttatcatgt tgcgacttcg tccacagacc cttttttcaa agggaggttt
    10201 tgtctcagta ccgcctgtta tcgctgcgcg tgtgtcagga gtgcctgtct ttattcacga
    10261 atctgacctg tctatgggct tggccaataa aatcgcctat aaatttgcga ctaagatgta
    10321 ttcaaccttt gaacaagctt cgagtttggc taaggtcgag catgtgggag cggtgaccaa
    10381 ggtttcggac aaaaacactc cagaaccaga tgaattggtg gatattcaaa cccactttaa
    10441 tcccaaattg ccaactgtat tgtttgtcgg cggttctgca ggcgctcgtg tctttaacca
    10501 attggtgaca gaccataaga aagaactaac agagcgctac aatattatca atctaactgg
    10561 agattctagt ctgaatgagt tgaggcaaaa tctttttcgt gttgactatg tgaccgatct
    10621 ctatcaaccc ttgatggaat tggctgatat tgttgtgaca cgaggtggtg ccaatacgat
    10681 ttttgagctc ttggcgatag caaaattgca tgtcattgtt ccgcttggtc gtgaagctag
    10741 tcgtggtgac cagattgaaa atgcagctta ctttgtaaaa aaaggctatg cagaagacct
    10801 tcaagaaagc gatttgacct tggatagttt ggaagagaag ctttctcact tactaagtca
    10861 caaggaagat taccaagcta agatgaaagc ttctaaggaa ttgaaatctc tagcagattt
    10921 ttatcaattg ttgaaaaaag atttatcata aggaaagtaa atgtcaaaag ataagaaaaa
    10981 tgaggacaaa gaaaccctcg aagaattgaa agagttatca gaatggcaga aacgaaacca
    11041 agaatatcta aaaaagaagg ctgaagaaga ggcggctcta gctgaggaga aggaaaagga
    11101 aagacaagct cgaatgggag aagaatctga gaagtcagag gataaacagg accaggagag
    11161 tgaaacagac caggaagatt cagaatcagc taaggaagag tctgaagaaa aagtagcatc
    11221 ctcagaggct gacaaagaga aagaagagaa agaagaacca gagtctaaag agaaggagga
    11281 acaggataaa aagctttcta aaaaggctac aaaggaaaaa ccagccaaag caaagattcc
    11341 tggtatccat atcttgcgag ccttcacgat tttatttcca agtctgcttt tattgattgt
    11401 ctctgcctac ttgctcagtc cttatgcgac catgaaagat attcgtgttg agggaacagt
    11461 gcaaactaca gctgatgata ttcgacaggc ttcaggcatt caggattcgg attatacgat
    11521 taatcttctg ctagacaagg caaaatatga aaagcagatt aagtctaact attgggttga
    11581 atcagctcaa cttgtctatc aatttccaac taagttcact attaaggtca aggaatatga
    11641 tattgtggcc tactatattt ctggtgaaaa tcattatcct attctttcca gtggtcagct
    11701 tgagactagt tctgtgagtc tgaacagttt accagaaact tatttatcag ttctctttaa
    11761 tgatagtgaa caaatcaagg tttttgtctc agaacttgct caaattagcc cagaactcaa
    11821 ggcggctatc caaaaggtgg aattagcccc aagcaaggtg acatccgatt taattcgatt
    11881 gaccatgaat gattcggacg aagtcttggt tcctctatct gaaatgagta agaaactgcc
    11941 atattacagt aagattaagc cacaattgtc agaaccgagt gtggtcgaca tggaagctgg
    12001 aatttacagt tacactgtgg cggataaatt aattatggag gctgaggaaa aagccaaaca
    12061 agaggccaag gaagccgaga aaaaacagga agaagaacag aaaaaacaag aggaagagag
    12121 caattgaaat caaacgaccc agcgctcatc gcgtcgctag gtttaccttt tctcttatag
    12181 ttctttagtg accatgtttt tacttgacaa gtgctagtag aaataataga ataatagaaa
    12241 acctttaaag cagtccagag aggcagctaa ggttagacgg tgaaagggtg gagactaccc
    12301 atttttcgtg gaaccttgct gttggcaggt tccttttttc gtggcttctg ttggccagac
    12361 tctctcacta gtaaaggtaa aaggagaaac ctatgcgaga acatcgtcca atcattgctc
    12421 ttgattttcc tagttttgag gcggtcaagg aatttttagc tcttttccca gcagaagaaa
    12481 gcctttatct caaggtaggg atggagcttt attacgcagc ggggcctgag attgtgtcct
    12541 acttaaaagg tttgggtcat agtgtctttt tggatctcaa acttcatgac attcctaata
    12601 cagtcaagtc agcaatgaag atcctatctc agcttggtgt cgatatgacc aatgttcatg
    12661 cggctggtgg tgtagagatg atgaaggctg cgcgtgaagg tcttggaagt caagctaaat
    12721 tgatcgctgt aactcagctc acatcaacgt cagaagctca gatgcaggag tttcaaaata
    12781 tccaaaccag tctgcaagag tctgtgattc actatgccaa gaagacagct gaagctggct
    12841 tggatggtgt tgtttgctcg gctcaggaag tacaagtcat caagcaggct accaatccag
    12901 attttatctg tctgacacca gggattcgtc cagctggtgt tgcagttgga gatcaaaaac
    12961 gagtcatgac acctgctgat gcctatcaaa tcggcagtga ctatatcgta gtgggacgtc
    13021 ccattatcca agctgaggat cctgttgcag cttatcatac catcaaggat gaatggacac
    13081 aggactggaa ttaaagaact agattagaaa aataaaagga gaataccatg acacttgcta
    13141 aagatatcgc tagccacctc ttgaaaatcc aagccgttta cctcaaacca gaggaaccct
    13201 tcacttgggc atctggtatc aagtcaccga tttacactga taatcgtgtg acactagcct
    13261 atccagaaac tcgtacccta attgaaaatg gttttgtgga tgctatcaaa gaagcctttc
    13321 ctgaagtaga agtgattgca ggaactgcaa cagcagggat tccacacgga gccattattg
    13381 ctgataagat gaacttgcct tttgcctaca tccgtagcaa accaaaagac cacggagctg
    13441 gtaatcaaat cgaaggtcgc gtagctcaag gtcaaaaaat ggtagtggtt gaagacctta
    13501 tttcaacggg tggttcagtt cttgaagctg tagcagcagc caagcgagaa ggagcagatg
    13561 tacttggagt tgtagcgatt ttcagctacc aattgccaaa agcagataag aactttgcag
    13621 atgctggtgt taaacttgtg acgctttcaa actatagcga gcttatccat ctagcccaag
    13681 aagaaggtta catcacgcca gagggccttg atcttctaaa acgctttaaa gaagaccaag
    13741 aaaattggca agaaggttag gtcagtaaga taaagagaga cgaggctacc gagcctcttt
    13801 taccatttta ttta
//
LOCUS       DAVKGU010000051        13568 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKGU010000051 DAVKGU010000000
VERSION     DAVKGU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.051"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(140..880)
                     /locus_tag="VW077_001363"
     CDS             complement(140..880)
                     /locus_tag="VW077_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9455374.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDETPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(1200..1553)
                     /gene="rsfS"
                     /locus_tag="VW077_001364"
     CDS             complement(1200..1553)
                     /gene="rsfS"
                     /locus_tag="VW077_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9455375.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(1566..2066)
                     /locus_tag="VW077_001365"
     CDS             complement(1566..2066)
                     /locus_tag="VW077_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9455376.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(2067..2660)
                     /gene="yqeK"
                     /locus_tag="VW077_001366"
     CDS             complement(2067..2660)
                     /gene="yqeK"
                     /locus_tag="VW077_001366"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9455377.1"
                     /translation="MSYQDYINCSREALLEKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIANLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(2660..3289)
                     /locus_tag="VW077_001367"
     CDS             complement(2660..3289)
                     /locus_tag="VW077_001367"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9455378.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(3341..3652)
                     /gene="yhbY"
                     /locus_tag="VW077_001368"
     CDS             complement(3341..3652)
                     /gene="yhbY"
                     /locus_tag="VW077_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9455379.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(3866..4972)
                     /gene="yqeH"
                     /locus_tag="VW077_001369"
     CDS             complement(3866..4972)
                     /gene="yqeH"
                     /locus_tag="VW077_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9455380.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(4975..5565)
                     /locus_tag="VW077_001370"
     CDS             complement(4975..5565)
                     /locus_tag="VW077_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9455381.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(5517..6425)
                     /locus_tag="VW077_001371"
     CDS             complement(5517..6425)
                     /locus_tag="VW077_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9455382.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTYEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(6482..7042)
                     /locus_tag="VW077_001372"
     CDS             complement(6482..7042)
                     /locus_tag="VW077_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9455383.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            7181..8388
                     /gene="sstT"
                     /locus_tag="VW077_001373"
                     /pseudo
     CDS             7181..8388
                     /gene="sstT"
                     /locus_tag="VW077_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            8456..9409
                     /locus_tag="VW077_001374"
     CDS             8456..9409
                     /locus_tag="VW077_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9455384.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            9552..9932
                     /locus_tag="VW077_001375"
     CDS             9552..9932
                     /locus_tag="VW077_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9455385.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            10032..10253
                     /locus_tag="VW077_001376"
     CDS             10032..10253
                     /locus_tag="VW077_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9455386.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            10270..10584
                     /gene="trxA"
                     /locus_tag="VW077_001377"
     CDS             10270..10584
                     /gene="trxA"
                     /locus_tag="VW077_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9455387.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            10889..11422
                     /gene="queF"
                     /locus_tag="VW077_001378"
     CDS             10889..11422
                     /gene="queF"
                     /locus_tag="VW077_001378"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9455388.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEDLA
                     EQRLFQHDLYPEKIDNR"
     gene            11472..11741
                     /locus_tag="VW077_001379"
     CDS             11472..11741
                     /locus_tag="VW077_001379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455389.1"
                     /translation="MLKAVFEVIDKTDEVSSKHCFEVADRTDEVSLKHCFEVVDRTDE
                     VSLKHCFEVADRTDVVSNHTYGKVKLTWFEEIFEEYHTKKPCSSR"
     gene            complement(11766..12434)
                     /locus_tag="VW077_001380"
     CDS             complement(11766..12434)
                     /locus_tag="VW077_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9455390.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEK"
     gene            complement(12545..13336)
                     /locus_tag="VW077_001381"
                     /pseudo
     CDS             complement(12545..13336)
                     /locus_tag="VW077_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
BASE COUNT         3928 a         3019 c         2604 g         4017 t
ORIGIN      
        1 taagtcgtta taaatttgct ttaacttttg ataaatttta ggtatattgg taattacttc
       61 tttttcaaga atatcaccaa atggtattgg tatattcatt ttcccttacc tcctaagcat
      121 aagattaaca atatttgatt tacttctgcg ccacaaaaaa ccaacgggtg ctagtttctg
      181 ttggctcctt atcctcaaag tctgcataga gtttgaagga cttaaatcca gcctgttcca
      241 gcaagatatc ataggtcaag atctcatagg tcctctcctc atgtacttca tcgtggcgac
      301 taaaggaacc gtcagcctcc ttgataaaga aagtcagctc atgcacgatg gagtgaggag
      361 tttcgtcctc atatgtatcc caaagcatgg caaaatcttc cgcattttca tggtaggaat
      421 agccagggaa tacttcatct gtctggtagg tcgagtgcac gtcaaagatg aaaactccct
      481 cttcattcag cgcattgtac acgtccttaa agacgtcccc tacttccacc tcatcctgca
      541 tatagcagat agagtccgaa taacacgtga caaaatcgta tttccctgcc ttggacaaat
      601 tcagcatatt accttctata aaagcaatct tttgcttggc tgaagttgct ctcttctccg
      661 caatcttcaa catatccgca ctcaagtcaa gtccagtcac atcaaaacca gcctgagaga
      721 agcgcactga ttgaattcct gtcccacaag ccagttccaa gagtttcttt ctctccttgg
      781 tcttaggcaa atgacgcaga gaaaaattcg tccatttgtc gtataaacta tcgtccatga
      841 ccgcatcata gacagccgca aaggtttcat aagtcgccat aaaacatgat accaagtccg
      901 cccgtaattc gatagaaaat taggagcctt acgatggagc cgagctccaa ggaaggctat
      961 ctattttccg ataattacag gacgggttca aatcctttct aagatacaaa gagccttaaa
     1021 agcaaagagt acataggaaa ctttcttccc tatcgtcaga tacaaagaaa gttacttttt
     1081 atctcagctt ttagctcgtg ttcagtttcg aaatgaaaca aacacgaggc cctttacctt
     1141 tctatcaatt tttctaaaat aagcaaagaa acccagttga ctacaactga gttcatcttc
     1201 taagcaagag cttctgaaat atctactgaa ttcgcctcat gccatagctt ctctagatta
     1261 taatgggcac gcatttcctc tgaaaagata tgcacgacaa cagcaccgag gtccagtaag
     1321 acccagcctc cagctgcatc gccttcgaca tggctacctt taaagcccgc ttgagctact
     1381 ttttcacgga tattagctgc gatagcgtcc aactgacggc tattcattga gctagtgatg
     1441 acaaagtagt ccgtcacact agtcaaatct tgtacatcaa gtgcaaggat atcctccgca
     1501 cgtttctcat cagccgcttt cacgactagt tctagtaatt ctttttcgtt catttaatcc
     1561 tctttttaga aaatatgttt gtaatctaga acatctgcaa agcgctcctc ccaaatgtcc
     1621 tcataaaatt cattaatcgt ttctgctgga atgtcatccc catcaaaggt tgtgacagca
     1681 ccttctggaa caataagctg atagccatat tcaaaggcaa ccttgacaga ggtatccaca
     1741 caatattctg tctgcatacc acataaaact aatttttcaa tcccctgctt atccaagtat
     1801 tcttttaagc cagtttcttt gaaaatactg ttatacttct tctgaaagac cttttcagca
     1861 ggttttcgat ttaaaagcgc agataactgc caatcttctg atgtttgagc ttcagagttc
     1921 tcaatatgtt gaacatagat aatttcaata ttcttgcttc tagcctggtt ttgtaaataa
     1981 gaaatttttc ccaatagact ttttgtctgg aacccagttt ctactaaaac attctgaaca
     2041 tcaatgatta taaaagccgt cttcatttag ttctctttca aatagtgcac aaaggcgtta
     2101 taggtttcaa gggtttgggg atagatgggg aatccctgat gagctagata ctccacggta
     2161 cgagctgttt cgtaggccac cgccttattg agcgatagat ttgcaatctc acgcgccaca
     2221 tctactccag gaaaggctcg attgtgctcg atatagtctg cgacgtagat gaccttatct
     2281 aggtctgtca tttgaccagc accgactgta tggacttcaa tggctcgcag gatttctgaa
     2341 tcatgcaaat ccaaatcttc ctgaattttg taaattccga ccataccatg ccagacatta
     2401 ttgccccagt ttttgaggtc agggtctagc tggtaacggt caatcaaggc tagaaattcc
     2461 tgatttgaca actttttagc atagtcatga agaagacctg ctagacttgc tttctcaaca
     2521 tctacaccaa agcgctgagc taattccata gcggcacgct ccacacctag acaatgggtt
     2581 agacgtttct caggtagaaa ctctgccatt ttttccaaca aggcctcacg ggagcagttg
     2641 atatagtctt gataggacat cagtagagcc cctccttctc gatgtagtct agcactggct
     2701 gaggtaggag aaagttgggt ttccgacctt gggcaaggaa gtcccgcacc atgctggacg
     2761 agatatccat gagcggtacg tccacccaga taactggata ggaagtccct accttgtagc
     2821 gtggacgctg aacccccaca aactgaacca tgtcaaccag ttcatcaatt cggtaccact
     2881 taggcagata gtcaaccatg tcggcaccga tgataaagta ataatccgta tctggattct
     2941 tctctgtcaa aatcttcatg gtgtcgtagg tgtaggaaat acccttgcgc tccaactcaa
     3001 tggtttcaat gactaggccg tcaattccct caattgccaa ctcaagcatc ttgagacgat
     3061 ggtgttcagg gatggtttcc tttttatcaa cgtgaggagg ttggtattca ggcatgagaa
     3121 gaacttgatc cagtcccaac tgttgccgta cttgatccgc aacaatgaga tgggcattgt
     3181 gaacagggtt aaaattcccc cctaaaatcc caacttgttt gcgttttttc tccttgattt
     3241 ctggctccaa ctctaccttg gtaaagggag tcaatagttc gattgtcata ggctagtccc
     3301 ctttatttct ctataaaaac agttatttgg agtttcggtc ttagatttct ttgactttct
     3361 tagaaatctt gcgattttct ttcttgctag attgcttaaa caaaatcaag atgcgtccta
     3421 ttttttggac tgtatccaca ccgatttctt cttccaaaat ttcagctact tcgtggatgt
     3481 tttcatctgt gttttgtaag agagtaacct tgattaattc acgcgcatca agagcttgac
     3541 ggacgctggt tttgatttgg tcgttgagtc catttttccc gatttggatg ataggtttga
     3601 gggtgtgtgc ctggctgttg aggaaggcac gttgttttga tgttaatgac ataattttct
     3661 tccttgtttt ttgatagtta ttttaggtgg tgagggacgg tcggaactaa ttttccaaag
     3721 atttcatctt tgaaaaatgg atttcctgac ctcacaattg agccttggtc tcaattgtgc
     3781 cccttgccaa tctacagatt ggcaatcaca ccacggcaat aactatcgct atatgagctc
     3841 actttgttcg ctcagcgatt ttgttttaaa taattgcttt tcgtgtgacg acggcgacgc
     3901 cttctggtgc ccagacggcg acttttgcta tgcctgttac acgaatccag cctaggcctg
     3961 agatgactag gtctgtctta tctttaatgg taaagacatg ctggactagc cttgggaaat
     4021 cttctttttc cttgctattt ggtggtgtca gaagagttcc caggtgctta tcgtagaaag
     4081 cactagctcc ttcaagcttg ctacgatgga gtttgagttc attatcaaag aaagcagtaa
     4141 atccttgctt ttctcctgct ataaagtcaa agcgtcccaa accacctaaa aatagggttt
     4201 gctcaggatt aagctgatag gtcttaggct tgatttcctt tttaggactg acatacttga
     4261 ggtttttggc cgtcaagtag tgagccatct ggtggcggtg gataattccc ggcgtatcgt
     4321 aaatataaga tccgtcgtca agcggaatct ctattttgtc caaggttgta cctgggaagc
     4381 gtgaagtagt gatgacattc tgatcacccg tgatttcttg gataatagca ttgattagag
     4441 ttgattttcc aacgttggtc acaccgacca catagacatc gcggccctta cggtagtgtt
     4501 caatcttgtc aatgacttcc ttaatggcat gtttattttg tgctgaagtt aggaccacat
     4561 cgactggacg aagaccttct tcatgggcac gtttcatgag ccactggcta atcttaccag
     4621 acttaactga cttaggaagg atatcttttt tatttcctac caagaggaca tcattgcccg
     4681 agacgaaacg tggtaaacct gggatgacag atccattaaa atcaaagata tcaatgacat
     4741 tgaccactaa ggcatcactg tctcccacct cgtgcaagag cttgaggaaa tcatcgttcg
     4801 tcaactggac atctgtgatt tcattgtagt ggcggagacg gaaacagcgt tggcaataga
     4861 cttcgccagt ctccaaacct ttttcaagtg ccgactgggg ggtaaaacca agaccagctt
     4921 tatctgtcgt ctgaatggtt gctccacaac caatacagag aatttcttcc atagttaaat
     4981 tcctttttta tatgtaatcg gtccgtactt ttcagtgatt tttctcataa cacgacgctc
     5041 acgagttcgg ttaatctgcg ttttgattga gtcatgttgg accaagggtt tgactaaaat
     5101 tgaccgaatc cctgcacggt gggctgctcg tatatctgtc atgagctggt cgccaaccat
     5161 gaccacttcc tttttgtcat agtggaattc cttcatagca cggtcaatac caaatgtgaa
     5221 gggcttcaag gcccagtaaa cgtaatcaat cccaaatttc tcaactgctc gttgaacgcg
     5281 ttttttggtg ttatttgaca ctacgataat gccaataccc gcgtcccgaa ggtcatgtag
     5341 ccattgcttc atctctggcg ttccatcagg gttgttccaa gcaatgaggg tattatccaa
     5401 atcgaccaaa acagccttga ttccctgcgc ctgcaggctt gggactgtca gatcatagac
     5461 tgcttccaca gcaaaatctg gtatataatt ttcaatcgcc attctggctc cttttcttaa
     5521 ctttttttcg caattttcct tagtaacaag gataaaacaa tccacaaacc tgcactagcc
     5581 aaactgatat agagccaagc atggggctca tatgttaaag gtaagggaac attcattccg
     5641 aaaaagcctg tcacgactgc caaaacagct agcaagactg aaatgatagt caaagttgtc
     5701 aaattgtcat tcagattatt gtttagaata ttgttgtaag aggctgaaag ctgctgtaaa
     5761 atctgagaga ttaggtctgt catggatacc agctgatgag cctcaatcat ggcatcatca
     5821 aactgttctc tctcaatctc atcaaaacta cgatacaagg catgaccttg aatatgctct
     5881 aacaaaatcc gattttgttt ggcagctgcc gttagataaa ccataccagt ctccaaatca
     5941 gacagggcaa agaggttttt cttggtagtt cgctggcgca agaggtcatt gacctcatcc
     6001 ctactcttgt ccatctgctc aatgacagga tagtaggcat tgctgatgat ttccagactg
     6061 gcaaagagaa acttataaat cgaaagcgtg tcatggctct ccagataacg agtcatctgt
     6121 tcaatgacat aggcgttctt ggtattacta atggtaatca ggcgacgatg ctcgacaata
     6181 aaggtcatgg gaaaagtctc atagtaggcc ttgtcctttt ttacgtctag gacattatag
     6241 ataaaggtaa ccgtcccact ttcacggtgg tagtccatgt gggcgcgctc gtttctatcc
     6301 agtgcgtatt caatggtttc cttgtccaaa ccgtaaattt cagaaaggtc ttctagttta
     6361 ttcaactttc ctaggtctag gtctatccag gtacaaccat tgcccaactg cttttctaaa
     6421 accatctttt ctccttatta aactccctct attgtaccac aaattactta aaataacagg
     6481 tctagtctgt gcgtgagaaa ttgctaacaa ctgtgatatt tcggttaggg acaaagtgaa
     6541 gggcttgctc ctcgctacga agctgtgtcg tacgaattcc cacactgaca accttacccg
     6601 atacagtaat cggtccattt gtcagaacga cctcatctcc cacatccagt tgacgttcaa
     6661 agaggatgaa aaagccattg atgacatcag acagaaagcc ttgggctccc ataccaatcg
     6721 ctaccccagc aataccagct ccagccagca aactagaaac tggcaaacct aaaatcgaca
     6781 aaatgcagta gagtaaaaag aaatatagcg tataattaaa cacattttct agtaaacgtg
     6841 agatggtttt ttggcgtccg acatcatgac gagacatttt tagagaaggt ttgacaattc
     6901 tctgcaccat ggtatgaagc atttttttag caatataaaa tacaatcaaa agcagtaaaa
     6961 gagaaatgac cttggttaga atattctcga taatggttgt cacatctagc ttttcaatat
     7021 aagcctgaat aaatttttgc atataaaact cctttatttc attataccat attttcatca
     7081 gtctcattct ccataaatat tcaaaaaata gacagaaaat tcttgatttt gtctatcatt
     7141 tatactataa tcataatcac tacacaaaaa ggagattatt atgaaaagaa tcattcatgc
     7201 ttggaataag gcaagccttg tcaagcgtat cttgattggt atgctttgtg gggaatccta
     7261 ggactgaccc ttcctaatct ctcagggatt gggctactcg gggatttatt cgttggaggt
     7321 ctaaaagctg ttgctcctat cctagtcttt gccctcgttg ccaatgccct ttccaacatc
     7381 aaaagggaca agatagcaat atgaaaactg ttgttttctt gtatatccta tgatagggac
     7441 tttcgccgct gctcttgtag ctgtactagc aagtttcatc gtccctattg aaattaccct
     7501 aaatagtgcc aatactgaaa ttgcaccacc agatggggtt gggcaggttc tcagcaacct
     7561 cttgctcaaa ctggttgaca gcccagtcaa cgccctgttt actgctaact atattagaat
     7621 cttatcttgg gcagtcattt ttggaatcgc tatgagagaa gccagtaaaa atagtaaaga
     7681 attgctaaaa actatcgctg acgtgacttc taaaattgtc gaatggatca tcaatctggc
     7741 tccatttgga agccttggtc ttgtttttaa aaccatttct gacaagggag tcggaagcct
     7801 taccaactac ggtattttat tggttctatt agtaacgact atgctttttg ttgcccttgt
     7861 ggtcaccctt tgattgcctt cttctttatg agacgcaatc cttaccctct agtttggaac
     7921 tgcctccgtg tcagcggtgt gacagccttt ttcactcgta gttctgcggc taacattcct
     7981 gtcaacatga aactctgcca tgaccttgga ctcaacccag atacctattc tgtttctatc
     8041 ccactcggtt ctactatcaa tatggctgga gtagcgatta ccattaacgt tttgaccctt
     8101 gctgcagtta acactcttgg aattcctgtt gactttgcca cagcctttgt cctcagtgtg
     8161 gtagcagcta tctcagcctg tggtgcttca ggtattgccg gaggttccct ccttcttatc
     8221 ccagttgctt gtagcctttt cggtatttct aacgatattg ccatacaagt tgttggggtt
     8281 ggttttgtga ttggtgtcat ccaagactca tgtgaaacag cccttaactc ttctacagat
     8341 gtcctcttta ccgccgttgc cgaatacgca gcaacccgta aaaaataact catcaaggca
     8401 agcctgctca tgtcttgtct tttacgcttt tattctaact tattaggaaa ttcttatgtc
     8461 tattagccaa cgtacgacca agctcatctt agctacctgt cttgcctgcc tgcttgctta
     8521 ttttctcaat ctttcatcag cagtttcggc tggaattatc gctctcttga gcctatctga
     8581 tacgcgtaga agtactttaa aactggctcg caatcgtctt ttttctatgc ttctagctct
     8641 ggctatcggt gttctagctt ttcacttgag cggatttcat atctggagtc tcggcctcta
     8701 tctggccttc tacgttcctt tagcctacaa gatgggctgg gaaattggca tcacaccaag
     8761 cactgttttg gttagccatc tcttggttca agagtcaacc tctccagacc ttctagtcaa
     8821 tgaattcctt ctctttgcta ttggtacagg atttgccttg cttgttaatc tctatatgcc
     8881 ttcacgagaa gaggaaatcc agcactacca cacgctggtg gaagaaaagt taaaagatat
     8941 cctccagcgc ttcaaatact atttatccag aggagacgga cgcaaccgag cacagctggt
     9001 agcagaatta gacacgcttt tgaaagaagc cctcagactg gtctatttgg atcactctga
     9061 ccacctcttt caccagaccg actaccatat ccactacttt gagatgagac aacgacaaag
     9121 tcgtatcctg agaaacatgg cccaacagat taacacttgt caccttgccg ccagtgaaag
     9181 cctgatctta gcgcaactct tttcaaaaat tgcaggtcaa ctgagccaga ccaatcctgc
     9241 ttctgatttg ctagatgaaa ttgaacgtta tctggaagtc ttccggaacc gcagtctgcc
     9301 caagacaaga gaagaatttg aaacccgcgc cacccttctt caactcctac gtgaagccaa
     9361 aaccttcatc caagtaaaag ttgattttta ccaaaaatat agacagtaaa aaataaaacc
     9421 actaaaaaac agagcttgcc agcattcttg aaaagtagta taattattgc atgcgcaatc
     9481 atcttgtgac gcagagactg cccgttaatg gactttcttc tatttatgac tctaaaaaga
     9541 aaggagacac tatgacctat ttggaaaaat ggtttgactt caatcggcgt cagaaagaaa
     9601 ttgaaagtct cttggaagag accattgccc aacagagcga gcaaagtctg accttgaaag
     9661 agttctacct gctctactat ctggacttgg ctgaagaaaa atctctacgc cagattgacc
     9721 tgccagataa acttcatctg agcccgagcg cagtttctag gatggtggct cgcttggaag
     9781 ctaaaaattg cggtctactc agtcgcatgt gttgccatca agatagacgc tctagtttta
     9841 tctgcctgac aaatgatggg caaaagacac tggcctcgct acaaaagact gtcgaagaaa
     9901 gcttggaagc aggtttggat ttcttgattt aagatgattt ttaaaaaaag ttgcgtgcgc
     9961 aatcattttt cttgacattc tcttttataa ggagtaaaat aaagttagca ttaaacaaag
    10021 gagttttaaa tatgattgaa attacctata tagatgccag caagaacgaa agaactgtga
    10081 ctttcgagtc ttatgaagac tttgaacgtt cgcaacaagc ttgccttatc ggcgtcgcag
    10141 actactaccc tgtccaaaaa ttaacttaca agggtcataa tttggactac catgggactt
    10201 atggagatat cttcttctat ctcatgaaac aagatttaag ccaatataac taaaaaaagg
    10261 agaaatacaa tggcaaaagc aattacagat gcaacattcg aacaagaaac aaaagatggt
    10321 ttggtcttag tagacttctg ggcaacttgg tgtggtccat gtcgtatgca aggtccaatc
    10381 ttggacaaat tgtctgaaga actttcagaa gatgtcttga aaatcgttaa aatggacgtt
    10441 gatgaaaatc caaacacagc tcgtgctttt ggaatcatgt ctattccaac tcttctcttc
    10501 aaaaaagacg gccaagttgt caaacaagtt gcaggtgttc acacagcaga acaaatcaag
    10561 gccatcattg ctgaattgag ctaatcaaac gagagaccaa gtactttctt tggtctcttt
    10621 tttcttgccc tttgccattt ttcaaaaaat atgctagact atagtagatt gaaactagaa
    10681 tagtacacct ctacttctaa aacattgtta gaaatcgatt taactgtcct gatcgatttg
    10741 tcctgttctt atttcatttt actatataag tagaatatta cgatgtttgg gatatgccat
    10801 cgctaaaaaa actcgatttg gtatttttta gctccctaat aggagctttt tgcatgctct
    10861 gaacatttcc cattttggaa ggagtactat gaaacgtcaa tcagccttgg tcgtctttag
    10921 tggtggtcaa gattcaacaa cctgcctttt ctgggttatg caacactatg aaacagtcga
    10981 agctgtcacc tttgcctacg gccaacgtca tcacctcgaa attcaaattg ctagagaaat
    11041 cgctaaggaa cagggcattc gtcaccatat cctcgatatg tctctgctgg gacaaatcac
    11101 tgctcagcca gactttgcga cgattcatat ttcctacatt cctgacaagc tctgtgtcga
    11161 gtcaaaatcc ctcaaactat atctatttag ctaccgaaat cacggagatt tccacgaaaa
    11221 ctgtatcaac accatcggga aagacttggt caacttgcta gaccctcgct atttagaagt
    11281 ctggggaaaa ttcactccgc gcggtggcat ttcaatcgac ccctactaca actacggtaa
    11341 gcaaggaact aagtatgagg acttggcaga acaacgcctc ttccaacacg acctttatcc
    11401 agagaaaatt gacaaccgct aaagtcatac tcaatgaaaa tcaaagagca aactaggaag
    11461 ttagccgcag gttgctcaaa gcagtgtttg aggttataga taagactgac gaagtcagct
    11521 caaaacactg ttttgaggtt gcagatagaa ctgacgaagt cagcttaaaa cactgttttg
    11581 aggttgtaga tagaactgac gaagtcagct taaaacactg ttttgaggtt gcagatagaa
    11641 ctgacgtagt cagtaaccat acctacggca aggtaaagct gacgtggttt gaagagattt
    11701 tcgaagagta tcatacgaaa aagccctgtt cctcaagatg aggagcaagg ctttttgagt
    11761 ttcaattatt tttctgttcc aaggaaattt tttgcaacaa gggctgcaag aactccacca
    11821 gcgattggtg caaggatgaa aatccaaact tgttgaaggg ctgcgccgcc taccaagaca
    11881 gctggtgcca agctacgagc tgggtttact gaaagtccag taatcttcaa tccgacaaga
    11941 atcatcgcca tcaatgacaa accgattacc aaaccagcaa tcgcgccatt gcccttactt
    12001 tctgaagtca cagtcataat aactaagaca aacaagaaag ttgcaatgac ttcaaacaag
    12061 aaaccaccaa agacagtgac accgtttgcc aaggcatttt caccaagact agcagttgac
    12121 atacctgaat tagccaagag gaagaagact gcgccagaag cgatgaaagc tccaacaacc
    12181 tgaccaagga tgtagtttac aagttctgaa gatgacaaac gtttgtttac aaacatagca
    12241 atcgaaacag ccgggttcaa gtgagcacct gaaacagttc cgattgagta ggctgccacc
    12301 acgattgcca aaccaaaggc aaaggcgatt ccaaggtgac caaggccatc aagaccattt
    12361 ccaaaaacaa cagctcctgt cccaacgaac acaagcatga acgtaccgat taactcagca
    12421 acaaattttt tcattttctt tctccttttt tcaaaaacta gatactagtc tatcaaaagt
    12481 aggaaagggt ttcaagaaaa ttgattggaa attttttgaa aatcatagaa ctattagcta
    12541 atctctagta ttgaaaagac tggatagctt ctttcaactc atcttgtaaa ctatttctct
    12601 ggtcaagttg gacatagact tccaccagac aggatctaaa gttggaaaat ttgtaaaaat
    12661 cctccctttc ttctatcgga aaatcaacag tttttatcca agaagctact tgttcttgct
    12721 ccaacttccc ttataaaata ggttcataga tcactcttgc taaacgccaa tcctcatcat
    12781 ctgtaaagcg aatcgacatc cttttaaata gttggccaag tatatcaaat acttcatgaa
    12841 ctctgttttt aggaaagtct ggatgacaaa ccacctctgt cagtaaatcg gctccatgtg
    12901 caaaagcgtg aacccaacca tactgacttg agaaacctgt tgtatccttt tcttttgaaa
    12961 gatagtgcaa gccttgattt gaaaggtcat tacgaaatcc tgcatttaat tcctgataaa
    13021 aaaccgattg ctggttggca tcgtcagaca agagagttgc ataaataagc gccctaaaag
    13081 aacgttcaag tgttggcagg cctatcttat caatctcttt atctaaccct ccatcagatg
    13141 aaaccccttc agcaatgaaa tgaaattgtt cctgtgtaaa tagctcttcc tgaatccctc
    13201 tagcaaagct tgtaaaaaca aggtcatcac gaatttctgg agaaggatct cccaaatgat
    13261 caagcaacca ctggatttcc tcctggttat aatttggttt ttcttctgct atttttctta
    13321 gtaaatcttg atacatggtc aatacctcta catttctagc aactgttcaa aaaggcagtc
    13381 ttaaatgacc ttaccaattt gtaaaattaa gttaaaaaga aaaagacatt tatattcaga
    13441 gaaacagcat atttctgaaa agatatataa atgatttatt ccatattaca acaaataaac
    13501 ttgtaatgat agaaccttat cacaaaaatc acattcctgt ctcaaaaatt gcgaaaaact
    13561 agttataa
//
LOCUS       DAVKGU010000052        12772 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKGU010000052 DAVKGU010000000
VERSION     DAVKGU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.052"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(421..2148)
                     /gene="ezrA"
                     /locus_tag="VW077_001382"
     CDS             complement(421..2148)
                     /gene="ezrA"
                     /locus_tag="VW077_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9455391.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEEKKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPPQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2230..4176)
                     /gene="gyrB"
                     /locus_tag="VW077_001383"
     CDS             complement(2230..4176)
                     /gene="gyrB"
                     /locus_tag="VW077_001383"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9455392.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4191..4754)
                     /locus_tag="VW077_001384"
     CDS             complement(4191..4754)
                     /locus_tag="VW077_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9455393.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4839..5393)
                     /locus_tag="VW077_001385"
     CDS             complement(4839..5393)
                     /locus_tag="VW077_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9455394.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5404..6627)
                     /locus_tag="VW077_001386"
     CDS             complement(5404..6627)
                     /locus_tag="VW077_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9455395.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFCMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6664..9114)
                     /locus_tag="VW077_001387"
     CDS             complement(6664..9114)
                     /locus_tag="VW077_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9455396.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVFLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9314..10243)
                     /locus_tag="VW077_001388"
     CDS             complement(9314..10243)
                     /locus_tag="VW077_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9455397.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKIDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQVFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10383..10670
                     /locus_tag="VW077_001389"
     CDS             10383..10670
                     /locus_tag="VW077_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9455398.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10704..12038)
                     /gene="ciaH"
                     /locus_tag="VW077_001390"
     CDS             complement(10704..12038)
                     /gene="ciaH"
                     /locus_tag="VW077_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9455399.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEINFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12028..12702)
                     /gene="ciaR"
                     /locus_tag="VW077_001391"
     CDS             complement(12028..12702)
                     /gene="ciaR"
                     /locus_tag="VW077_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9455400.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         3882 a         2728 c         2271 g         3891 t
ORIGIN      
        1 gaggcctatt taccgtggac taaagttgta caagaaaagt gcaaataaga aatctccaga
       61 ttaggaacta tccgtgagtt ctctagtctg gagatttttc aatatacttc gttattggac
      121 ggttaccaag ttactcaaag tacagatttg aagctgcaga taagactgac gaaatcactt
      181 taatactaaa tgaaaatcaa aaagcaaact aagaagctag cctcaggctg tacttgagta
      241 cggtaaggcg acgctgacgt gatttgaaga gattttcgaa gagtataaaa cactgttttg
      301 aggtggtgga taaatcggct gggtaaaaac tcaatctcag gagaaaatga agtaaatctc
      361 cccacaacta tagaaaaaga tttgattctg ctcctcacac aataaaatct ttttctttta
      421 ttaaaaacga atcgtttcac gtgttttctc atatgaggta acaaagcgat tagttacacc
      481 aggctctgcc acttccaatg cttgagaaat cttgtcaaat gaagcgtgat aatcaaattc
      541 tttttcaaaa atatctaaag cttcgttaaa tgcttcttga atgcgttcat caaatgagcg
      601 atagcggtta gaatattgca agagttgctc tgtcaaagtt gcatattgta caatattata
      661 agtttccgtt tctaaagctt ccatatcatt cgttgcaatt tcaagaactc gggtaacaga
      721 ttcaatgtta atcatttttt gttctaactc aaccattaaa tcctcggtat tattgcttgc
      781 cgtaaagaat aacttcaaga aagtttgtgg aatacctggc agattgcgtt tttccatgta
      841 tcgcttgata gtatggagac gattgacata aacattggcc ttttgacgtg cattaatatc
      901 atctttctca atttgtgtca ggcgctcact aactgaaatt tgctcatctt caatatcttt
      961 tagttgagtt tgtaaatcct caagattttc ttcaagaact gaataagctt ggggtggttc
     1021 ttcttgattt gaagttacct caacaatagc tgcctcaaaa ctctctaatt ctgtctgaat
     1081 acgacgaaca tggcttgcag ctgtctcagg aagtaaatag gtcttgttca aacgtgcaat
     1141 atcttctccc aataaagtat tattctcttt catatgttga agataagttg gaagagttgc
     1201 aagtagattt tccactactt tctgagcagc aatttctcga gtaaaaatat catacaaggc
     1261 attgatttcc tcttgtgcct gtccattctc atattcggca ttatccaatt ccaactgacg
     1321 aatattctct tggtttttct tgaatgcttc ataaagcaag tggaaacgcg cttcaatatc
     1381 cgtttcaaca aaatgataat tagcatcaat tagtttacga taaccggctt ccaaatcctg
     1441 taattgatct ggcaattctg tagaaagcgt cgtaaccaag gctggaacac gatccacaat
     1501 atgacttaag gccaaaatgt gattttctgt attatccaaa atcactgcgg cttccacagg
     1561 gtcacccgat gaattcaagg ttacaaattg tgaaaattca gattggatat tttctaattg
     1621 tttttcaatt tcatccaagg cttgaccata ctgttctgaa ttttcagcaa ctctatgctg
     1681 aagttcctca aataaatcca aagcatgaag aacacgacca ctatttttag attcttgctt
     1741 ctctaagtct gccaaagcat tgcgaattgc cgcaatatct tcttcaatca aagtaatttg
     1801 actctcaatt tggtcaattt gatgactggc cttgagaaaa cgaaatgaat ggttataacc
     1861 ttctgcttca aagagattat tttcaatatc ggcaaaagag ttgagagata aatcgaccca
     1921 tttttgattc cattcacgga aagccacttg actttgtcca atcaagtgca tattttttac
     1981 agcttctact tcatcattta ctggaagatt gtatagttct tctttttttt cttctagcgc
     2041 ctctaatctc ccctcgtttc gcttacgtag aaagattgcc actacataag ccagaactaa
     2101 aatgactgca attgcaacca ttaaataaat tagttgtcca ttagacatat caaactcctt
     2161 ttttacttga aacaatcgta acgattatat catatttttt agaccaaggg aactacttct
     2221 ccctattttt tagacatcaa gtgtactata gacagcattt tcttcgataa actcacgacg
     2281 aggctctact cgatccccca tcaacatatc aaagatttta tctgcttctg cagcatcatc
     2341 tacagaaact ctagccatca agcgatgttc gggatccatg gttgtttccc acagctgatg
     2401 atcatccatt tcacctagcc ccttataacg ctgaatagtc ggtttggtac gaccttcact
     2461 ataacgggct aaagcttctt ggagtttgat ttcttgatct gcacccggct ggatatattc
     2521 tttaatctcg cttccaacct tgacaccata gattggtggt tgggcaatat aaacataacc
     2581 agcttctagg attggtttca tataacgata aatcaaggtt aaaagaaggg tacgaatatg
     2641 ggctccatcg acatcggcat cggtcatcaa aacgagtttt tggtaacggg ctttcgaaac
     2701 atcaaattct gcgccaaatc ctgttcccat ggctgtgaaa agactacgaa tttcttcgtt
     2761 agctagaatc ttatccatac ttgctttttc aacgttcaaa atcttaccgc gaattggaag
     2821 gatagcctga aactcacggt tacgaccaga tttggctgat ccaccagctg agtctccttc
     2881 gacgatgaag agttctgttt cagcagggtt attagaagaa cagtctgcta gtttccctgg
     2941 aaggttggaa atttccaaac cagatttttt acgtgtgact tcacgcgcac gcttggcagc
     3001 cacacgagcc ttggcagcta aaatcccttt ttccacgata cgtttggcaa tctgtggatt
     3061 ttccatgagg aaatcggaga aggcttcact gaagaggcga ttggtaatct tgaccacttc
     3121 gctatttccc aatttggtct tggtttgtcc ttcaaactgt ggatttgggt gtttaactga
     3181 gataactgca gttaatcctt cgcgaacatc ttcccctgtt aaattatctt cattgtcttt
     3241 cagtaactta ttcttacgag cataatcatt gataacacga gtcagtgccg tacggaaacc
     3301 ttgttcatgc gttccacctt catgggtatg aatattattg gcgaaactca tgacattttc
     3361 atggtaacct gttgtgtact gcatggctac ctcaactgta atatcatcca tctcaccgtc
     3421 tgtatagatt ggtgtatcaa agattacatc cttgttctcg ttgatatatt caacgtaact
     3481 agcaatccca ccttcataat gataatgctt ggtttgttcc aaaccttggc gcttatcagt
     3541 gatagaaatt tgaagaccgc gatttagaaa ggccaactct tgaatccgtt tatttaattt
     3601 atcaaaatca aaggttgttg tttcagtaaa gatttttggg tccggtatga agtgaaccgt
     3661 tgttccagtt ctatctgtat ccccaaccac ctcaaggtct gcaacaacat gaccacgacg
     3721 gtattcttgg taatgaatct taccgttttt atggacatga acgtctaatt gagtggaaag
     3781 ggcattaact actgacgacc ccaccccgtg aagaccacct gaaaccttgt atccaccacc
     3841 gccgaacttt cctccagcat gaaggactgt aaagacggtc tcaacagcag gacggcctgt
     3901 tttttcctga atatcgactg ggataccacg cccatcatcc acaacagtaa tcgaatcatc
     3961 tggctcaata aaaacttgaa tatggctggc aaatcctgcc aaggcctcgt caattgagtt
     4021 atcaacaatt tcccagacta gatggtgaag accttctttt gaggttgatc caatgtacat
     4081 ccctggacgc atacgaacag cctctaagcc ctctaaaact tgaatttgac tggcatcata
     4141 atcctgtgcc tgcagatttt tgatttcttc tgtcatctta ttcctttttc ttatatgtct
     4201 aatacttgtc ttaaattcac gatactggta aacaagtggg tatcaagtcc tgcagcttga
     4261 cctgcttcga tatcaatcgg ccgatcacca atgacaagac cagagctaat ctgatacttt
     4321 tctcttaaat aaagcatgga ttcgggattt ggctttctct taaagcctga gctagaagtc
     4381 accacttctg taaaataagc tgctatagag gttttttcta aaatttccaa aacctgatca
     4441 tttcgatgag agaccaaaaa atgacggcca ccttgattta aaatgtcttc caataggtca
     4501 gaaactcctt caaataaaat cgggtgttca agctctctgg cttcattttc cttgtacttt
     4561 tctaaaaaat tctctaaatt gggagcgaat gtctcaatcg caaaaggagt agaaaccttt
     4621 aaagcttgat agacactgtc atggtcttgt gtgataccat acagtgccaa tgtttcaaca
     4681 aatgcagctg ttgaagtttc ataattatcc agtaaagttc cacctaaatc ccagatgtaa
     4741 tcgtgatatt tcataccctt cattatagca tttttttatg aaaaaagcga tgatttccct
     4801 gagttttcca cataaaaaac gagagaataa ctcccgtttt actgattttt accaaagaca
     4861 tctcgataga gcattcctgt tcgtaaactc tctgcgtccc ctcctagttg ctccaccaac
     4921 tcgtaggcaa aggcaagggc tgttgaagga ccccgactgg ttgtcaactg accatctacc
     4981 actactgttt ccttgacgta gtgaccatca aggatttgct cttgaacgcc gtcataacaa
     5041 gtgtatcgct tatttttcaa tatctctgct tgattgaggg caattggtgc cgcacaaatg
     5101 gctgctagtt tcttcccttc ttgctcgaag ctttgcaatt cttgaatcaa ggtctgatta
     5161 tcacgtaaat gtgcagaacc aggcatacct ccaggaagaa caatcatatc atagtctgat
     5221 aaatttccat caaagacatg atctgctctt acttggattg catgcgaacc cgttacttgc
     5281 tcttcaaaac caaccatatc acatgtgata ttggctcgac gcaagacatc tacaactgtc
     5341 aaggcttcaa tttcttcaaa gccctgagct aacataactg ctacttttac catgattttc
     5401 tccttattta atttgtttta atacaacctt tttccgtttg aatgggactt ttccgctctt
     5461 ttcttcagct agattagtct ggtaactcat cgataaaagc aaaccaacac caatcagatt
     5521 actgataata gctgatcccc cttgcgaaat gaaaggcaag ggaatccccg tcaaaggaag
     5581 tagtccagtc acagcaccga tattctcaaa gatgtggaag agcaacatca taatcaaacc
     5641 tgtggaaata taagtgtaga actggttatt tgatttaaga gtaatcttca acatacggta
     5701 aatcaacatg agatagaggg caataaccag gacagagcca ataaagccaa aatcttctgc
     5761 aataaccgta aaaatcatat ctgactctcg aactgggata agcagattcg aagcattaaa
     5821 tccctgacca aataagccac cactcccaat ggcaatctgc ccttgagcct gctggtaagt
     5881 cgttgtttgg gcaaactcaa agggattgag ccaagccaaa atccgattaa tttggtaggt
     5941 cggcattcca atctggtgaa gaaaagctcg tccgtccttg ctaataaaga tagctaagaa
     6001 accagcaact cctgttacag cagtcacaaa tactgggata ataattttcc aagaaacccc
     6061 tgataataaa acgattcctg agaaaatggc tacaaaaacc aaagccgtcc ccaagtcact
     6121 ttgaagtgct aaaagaacta ggactggaat ggtaaagaga atcatacaga aaattaacaa
     6181 aaagtccagc ggaaccgtgc gtctccattc cttatgtttc tttgtaaatt ggacaatgac
     6241 acgagccaac atgaggatat aggatatctt cataaattct gacggttgga atagggtaat
     6301 tccatttatt gatacccagt ttttggcacc cgttgatgca actaagcttg gattataaaa
     6361 tacaatcggc aagatcataa gtcccaagcc taaaatatat agaaaggggg tcaccttcca
     6421 aagaaattct gtattaaaga gcatgaccac aaaaccaatc acaagcccca aggcaatcca
     6481 ggcgacctgc tgccctaaaa tgggcagaat attattgggg taatcatgac taacggctat
     6541 atagatagcc accacaccga tgaccagtag aaaaaatact ggcaagagca aactgtaatc
     6601 gactcttgag tcgagagaac gtttcatata tactaacctt atactttcat acaatactat
     6661 ttatcaaagt tcattaaaaa atctatcaat agcctcgtca acttcggatc gagagatggt
     6721 tttaacagtc gcttcttctg ctagagatgc tactatttgt ttgccgtatc gttttccgac
     6781 gattctccta tccaaaataa gagttaagga acgttggtat tcacgtctca tacttcttcc
     6841 caaagcctgt tttaaacgaa taatggccat tggcaattga taatcataaa aggcattttt
     6901 cccttcttga ttcagttctt gattaatctt tttcgtcaag ggttcttgag gattttggaa
     6961 aggaagcctc ggtacaactt gaatcacaaa aggatggctt gaaaaatcaa ctccctccca
     7021 gaaacttgct gcaccaagca agatttgttg ttcacctttt tcaaagcgtt tctttagctg
     7081 atgaacatcc ccatttttat actgggccaa gtggctaact gtaagtaaat ccgatactgc
     7141 tagaagcatg tctttagcgg taaagagaac caaaatcggt tgctggaaag cttgaatttc
     7201 cactagtaaa gcagctacct ctctggcata gacttctaag gaggtttctg ttaccagagg
     7261 gaaatctttg accatgacca cttcttgttc ctgttttccc cgagattcaa tcttgacaaa
     7321 tttagcttca ggatagccta aaaggtctgc caaagaaacc ctctgactaa tctcaagagt
     7381 agccgatact cccaggactt ggcaactctc aggaagtaaa gaggacagaa gagtacggcc
     7441 tgattttgta gaagaaatct gaattttctt ttgactcgtt tcagttactt caagccagta
     7501 atcaccttca gctgtaaaat agcgaaccag ctcatcaaag ccttctactt ctaattctga
     7561 aaaatactga tggagattgt ccagagaatc taagatattt ttcctagatt taccagactg
     7621 aaattgttct atcaagtaga gacactcaaa gcgaatactt tctagtatcc gttgttgaac
     7681 cctgttttct tctcctacta aagccttatc aattaaatcg ataatagatt gtatatcgta
     7741 ggtctcttga agcagatttt ctagagctaa caaaatcttt tggacttcat caataatcag
     7801 taaacggtca ctgacaaatt caggattatc ttcaagtctg gtcacgagat aggcatgatt
     7861 agtcactaaa agcttgcaag tctctgccct ttcttgacta cgtttccaaa aatcttccgt
     7921 cacaaataag ctctgggatg ataaattccc atcatgacga aggtctgcta gaaaatgttg
     7981 gtaacggtag agttgcccga tttcatccaa atctcctgtc tctgtctcag taagccagac
     8041 caagacttgc attttaaagc gtctaaataa gcgattttca tcattttcct gcaaggaatg
     8101 ataaaaggca tccaacttca gataattttg tggtcccttt aagctatgaa tatctgtatg
     8161 gaacacttcc ttgagacgtt taccttcttc ttccatgatt tgattttgaa gaatctttgt
     8221 cggaacacta agaacaattt gtcgctcttt ggattgagat aaagcgggta agagatagcc
     8281 ataggttttc ccaatccctg tcggcgcttg aatcagaaag acaggttcat ctttcaatag
     8341 caagccaacc tctttagcaa aactttcttg ttcctccctc acttcaaggt tcaacagaga
     8401 aatattttta gaaaagtctt gagatagttt tcgtagctcc agagaagctg ccgttttctt
     8461 aaaatacaga ccttgaactt ggaccaagtc tggagaactc aggatagatt ggttgcgata
     8521 agtttcctca ataaccaggt aggactcata taggagagcg tcagccattt ccagcaagcg
     8581 ttccaagaga cctttaggaa gctgggtcat cttttcccgt aaaaaaagaa gtaattctgc
     8641 tgtagcttgg gcatctgaaa gggctgtgtg tgcgtgttta agaggaattc ctaattctcg
     8701 acacaaaatc ggcaagctat atttttccag ttcagggaaa aagacctggg ccaattcgac
     8761 cgtatcaaca cgagggtttc ttagctcata gccttcaaaa aataaatttt ccgccaagag
     8821 attagcatca aactgaacat tatgggctac aaaaatccca tcctccacca agtcaaatat
     8881 ttttctggca acttgcgaaa aatcaggtgc ttgcgccaga cgttggtctg tcaatcctgt
     8941 cagttctttg atatgagcat ccaagggttc atgtggattg acatccgtcg tatagtgatc
     9001 gacgatttct ccgtcctcaa tcacgacaat tcccacttgg ataattttag ccttactacc
     9061 tgtgctagtt gcctctaaat ccaccacaac atagcgatta ccaatcgttt tcattataaa
     9121 aaattatacc aaaaaaaggg ccatttggca cctgcaaaca taggataatt ttcaaactca
     9181 agaacactac ttttcttaaa aatcccatcg aatagagcat tctagcctgc tgatttttgt
     9241 aaaatagtga agaaatacga aaaattgaga gaagaaatgt ttgcatcttc cttctctcaa
     9301 cgaatcatgt attttatcta accatgtcag gatcgtagtc ataaaatcct gtatcaagga
     9361 aacggttttt agggtgaagt ttacgaactt cttcatccag caagaagact tgatcatggc
     9421 taaagttgcc ctcttgaatc aagttacgcg cttggataaa gagttttgca atctcaacaa
     9481 agttctgacc aggagtgtag agcccttcta gtttccagcg agtaaagcca tgctccacca
     9541 attctgttaa tttgatcatc aaatcaaggt cattgttggc aaagatatgg gtcccatgat
     9601 tatcttcaaa aatggaatag tggctctctg gatcacttgg ctcagccaag aagaggtcac
     9661 gtttatgcgt cttttcatca tcgatatgtg taaagttata gtagttttgc aagagtggac
     9721 gtttagaatg atggatgacg ctagcaccgt aaaccaaaac ttcagcagga atttccaaaa
     9781 tctctggcat tttgaaaagt tcagctgatg gaatttcacg cgccaaaaca gcctcagatg
     9841 cgccagcctt ttgtccccag aagttaatct gacgactgct agttaccatg gttgaagcat
     9901 cgtagatggt cttaaatgaa taaccatcgc ggttaactac gtaaaagacg cctgcatccc
     9961 caatcgtaat atagtctatc ttgatttctt ccaagaagtc taagaaaggc ttgatacggt
    10021 ccatcatatc ttggtgcata agagcattga ccgcaacgat caattcctta ccagcatcat
    10081 gaaccaactt agcgatttca cgtaattggt cataactaaa ggtcgttggc agacgaaggc
    10141 caaaatcttt ctcaccgaca tagatacggt ctacgccagc ttcgagtagt tgttcaactt
    10201 gttcaatact ttcagcagtt gctgtaatga caatcttttc cataagaaaa attataccac
    10261 atttctccaa aatcatccat gctttgaaaa tgaaaaggga ctttattctt tttgtaacct
    10321 ttctgtaata attctctgcc gaaaaaatga taaaataggt atgtactgtt aaggagagaa
    10381 ttatgcccgt aagaaaatta caatcctatg aggtagacta tcaagaagaa ttaaaccagc
    10441 agcttcctca ttaccaagct tatacacctg aagcgcaatc tgatgccaat ctcaaggaaa
    10501 tcctattttt tattaatatc gctgtttttt gtatctgcat tgctatcttt agttttatct
    10561 ttttagcatt aaaattatca actgctcttg cctttgccgc agcaatcgga ttcagcttac
    10621 ttgttttaaa agttcaacgg tctattatca aacgaaaacg tagaagataa atccaaaata
    10681 tcgccccagt tggagcgata tttttatttt ttctttttag atggtgtctg aatggcaatc
    10741 ttcacttcaa agattgttcc cttgggttta ttatctttga cagtaacagt tccttttaga
    10801 gcatctacaa tttgcttggc tagggataat cctaaaccaa aaccaccttt ttgccgggtt
    10861 ctagctttgt ctactcgata aaaacggtca aaaattttct ttttatcttc tgtcgaaata
    10921 ccgattccat tatcagaaac aagtaaataa agattgcgat cggtcgccga gataagaaaa
    10981 ttaatttcac catcctcctc agtatacttg acggcattat cgaaaagaat ggtcatcagt
    11041 tgtttcagaa gaagctgatc tgtgacaatt gttcgatgga tacgattttc aaaacggaag
    11101 acacgattat tttccgaagc aatcatctcg tagtttgtga aagttgtatt aaaaaagcta
    11161 gttggaactt ctgcaagctc cggcttaatc ccatcatctc tccgagctaa gttcagcaag
    11221 cttgtcgtta aaaaacgcat atttcggact tcttccaaac tcgatgcaat gctttcgctc
    11281 acatccataa tggtagcttc tggcttacga aaaagggtct ctaagcgatt ttgcaaaact
    11341 gcgagtggag ttcgtaactc atgactggca ttttccacaa aagactgttg cttctgcata
    11401 ctctcaagca ggggcctaac actgacccta gctagataga gactggcaag taaagacaaa
    11461 atccagaaac tagccatcac gaccacaatc aattgctcat gcttttgact agcctgctcc
    11521 aactgactgg tattaatcaa gacagcagca tacttgatat tggttgaaac cgaactaata
    11581 ttggtctcca tcaaaatcac acgatagatt tcttcctgcc catagctatt aaaaacctga
    11641 atctggtaga tatgtcctag ttctttcttt tctaacttaa tcttatccaa gcccaaaaat
    11701 cgatttccag aaagaagttg agtaaagtct ttatcaaaga gaatgacttc cgtattggaa
    11761 ctgacattag gttttatttc tactttacta gcgtccgccc tagcattttc taaatcttta
    11821 atctcttctg ttgccctatt tatagccagc tgaataactg cttgaggatt ttcactcagt
    11881 ccatgaagct tatcgtccac cgaagtataa agactcgaat gcatgacttg taaaataatc
    11941 agagtcattg tagaaaaaat cagggtgaag acaccgaagt tgcggataaa ataactaaag
    12001 tcatccgcat accatgtttt tttaagttta ctgaacatct tttaaaagat acccaacact
    12061 acgcaaggtt tgcaaattct ctgcaaaagt ggttcccttt aatttcttac ggacttttga
    12121 aacatagact tcgacaaccg aaatcgttgt atcactatca aatccccata gacggtcaaa
    12181 aatctgcgtc ttaggcaaaa tcacattttg attttgaagg aaataaacta gtaaatcgaa
    12241 ctctttcccc agcaattcga caggagtatc ttcaacttta acggtattgg ttgataaatt
    12301 aaccacgata ttcccataag tcaaggtgtt ttcattaaac ttccctgaac gtttgagaag
    12361 ggcctgaatc cgcattttaa gttcttctag gtagaaaggt ttggtcagat aatcatccgc
    12421 tcccagttca aatccatgtc ccttgtcatc caaactttcc ttggcagtca taatcagaac
    12481 tggtgtcgta attccctttt cacgcaattc ttttaagact tggaaaccat ttttttctgg
    12541 caacatcaaa tccagcaaaa tcaagtcata gacaccactc tcagcttcgt agagaccttc
    12601 ttctccatca aatacctgca taacatccgc aaaatcgtct aaaaagtcaa atactgaatt
    12661 tgacagacct aggtcatcct caaccaataa gatttttatc atgagaaact cctccttatt
    12721 aaaactatta taccaaattt gccttaaaaa aaactcaact ctctgcattt ta
//
LOCUS       DAVKGU010000053        12752 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKGU010000053 DAVKGU010000000
VERSION     DAVKGU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.053"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..440)
                     /locus_tag="VW077_001392"
     CDS             complement(<1..440)
                     /locus_tag="VW077_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9455401.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEA"
     gene            complement(440..1519)
                     /gene="prfA"
                     /locus_tag="VW077_001393"
     CDS             complement(440..1519)
                     /gene="prfA"
                     /locus_tag="VW077_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9455402.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(1516..2106)
                     /locus_tag="VW077_001394"
     CDS             complement(1516..2106)
                     /locus_tag="VW077_001394"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9455403.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            2229..2411
                     /locus_tag="VW077_001395"
     CDS             2229..2411
                     /locus_tag="VW077_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9455404.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            2548..3921
                     /gene="mnmE"
                     /locus_tag="VW077_001396"
     CDS             2548..3921
                     /gene="mnmE"
                     /locus_tag="VW077_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9455405.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQIEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(3968..4903)
                     /gene="dapA"
                     /locus_tag="VW077_001397"
     CDS             complement(3968..4903)
                     /gene="dapA"
                     /locus_tag="VW077_001397"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9455406.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(4959..6035)
                     /locus_tag="VW077_001398"
     CDS             complement(4959..6035)
                     /locus_tag="VW077_001398"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9455407.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEKSTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(6378..6450)
                     /locus_tag="VW077_001399"
     tRNA            complement(6378..6450)
                     /locus_tag="VW077_001399"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6415..6417),aa:Thr,seq:ggt)
     gene            complement(6550..7059)
                     /locus_tag="VW077_001400"
     CDS             complement(6550..7059)
                     /locus_tag="VW077_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9455408.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(7057..7153)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            7182..7613
                     /locus_tag="VW077_001401"
     CDS             7182..7613
                     /locus_tag="VW077_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9455409.1"
                     /translation="MKIPIQKFFNNEILAYLFFGIVTTLVAILLRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            7695..8072
                     /gene="mscL"
                     /locus_tag="VW077_001402"
     CDS             7695..8072
                     /gene="mscL"
                     /locus_tag="VW077_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9455410.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(8114..9208)
                     /gene="hemH"
                     /locus_tag="VW077_001403"
     CDS             complement(8114..9208)
                     /gene="hemH"
                     /locus_tag="VW077_001403"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9455411.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKAIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSKQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            9363..10586
                     /gene="pepT"
                     /locus_tag="VW077_001404"
     CDS             9363..10586
                     /gene="pepT"
                     /locus_tag="VW077_001404"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9455412.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIESIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(10622..11251)
                     /locus_tag="VW077_001405"
     CDS             complement(10622..11251)
                     /locus_tag="VW077_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455413.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLEPTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            11216..11437
                     /locus_tag="VW077_001406"
     CDS             11216..11437
                     /locus_tag="VW077_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455414.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(11574..11783)
                     /locus_tag="VW077_001407"
                     /pseudo
     CDS             complement(11574..11783)
                     /locus_tag="VW077_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
     gene            complement(11870..12133)
                     /locus_tag="VW077_001408"
     CDS             complement(11870..12133)
                     /locus_tag="VW077_001408"
                     /inference="COORDINATES: protein motif:HMM:NF016182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9455415.1"
                     /translation="MLEITLKSPYQFAHILFQPTIVPHGGHYHFIPESDLSAGELAAT
                     YVFNPNDIVRDTGDAYIVRHGVTITIFLSHLLTIPHHTLTRKR"
     gene            complement(12177..12737)
                     /locus_tag="VW077_001409"
                     /pseudo
     CDS             complement(12177..12737)
                     /locus_tag="VW077_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         3716 a         2690 c         2406 g         3940 t
ORIGIN      
        1 aaggcctctt gagaaacatc tgctgctgtc actgaccaat ctggtctatt ttttgctaga
       61 gcgagagcaa tagctccact acctgttcca atatccagaa ctgagagatt cgtctcaaga
      121 ttttcagtta gaataagctc cactaactcc tctgtttctg gacgaggaat caaaacccgt
      181 tcatccacct ttaaatgcat tccataaaaa tctgcctgtc caatgatgta ctgagctggc
      241 ttgtgagctg ctagttgctg gtaaatatct tctacaaatt gtttttcttc ctctgttgtc
      301 acctcctgct ggagggcaaa aataaagtct gtaaaagata gatttttcag actacgatag
      361 acaaaagaga ggctttccgc ttcctctcct tgtcttatca actcttcttc aaaatttgaa
      421 aataattgag ctaatttcat tatttgttta attcttctag tttttgtgtt tggtcataaa
      481 gcaccaaggc atccacaact tcgtccaatt taccagacaa aatcgtatct agtttttgga
      541 gggtcaagcc gatacggtgg tctgtgacac ggttttgagg gaagttataa gttcggatcc
      601 gttctgaacg gtcaccagta ccgattgtcg acttacgctc agcgtcttgt tcatcctgag
      661 caatctgagc aaagtggtca gcgacgcgtg cacggataat cttcatagcc ttctcacggt
      721 tcttctgctg ggtacgttct tcctgcatct caaccttgat attggttggc aagtgaacga
      781 tacgaacggc agtcgcaacc ttattgacgt tctgtccacc agcacctgat gcgtggtaaa
      841 tatcaacacg aagatctttt ggatcgatat cgtattctac ttcttcgact tcaggcatga
      901 caagaactgt ggcagttgag gtatgaacac gaccttggct ttctgtcacg gggacacgct
      961 gcacacggtg ggcacctgat tcgtacttaa gcttagagta tacagactga cctgaaacca
     1021 tagcaaccac ttctttaaac ccaccgacac cattcataga ggcttccatg acttcaaagc
     1081 gccagccttg ggcttccgca tacttttggt acatagttag caaatctcca gcgaaaagtg
     1141 ccgcttcgtc tccaccagct gctccacgga tttcaaggat gatattcttg tcatcgtttg
     1201 gatcctttgg aaggagcaaa attttcagtt tttcttcata ttcttctttt tcagccttgg
     1261 catctttgag ttcttgcttg gccatttctt ccaagtccgc atctccgcct gattccttaa
     1321 tcatctcttc ggcatcgacg atattttgaa ggacttgttt atactcacgg taggctatta
     1381 cggtatcacg attggaagct tcttcttttg aaagctccat aaaacgcttg gtgtctgaaa
     1441 cgacatcagg gtcactcagc aattctccta gttcttcata acggtcttct acagcttgta
     1501 gttgatcata gatgttcatt tttcttcatt ctccttattg atttcagggg caaaataatg
     1561 tttacggcaa accgagatat aggtttcatt accaccaatt tggatctgtt ctccatcgta
     1621 aacgggcagt ccatcctgtg ttcgcaacac catggtcgcc tttttcttgc aatactgaca
     1681 gatggtcttg atttcgtcaa tcttgtctgc taaaagcaag agatatttgg aaccttcgaa
     1741 caattcattg cgaaagtcat ttttcaagcc aaaagccatg acgggtatgt ctaactcgtc
     1801 cacaacacga gctaggtcat aaacatggtg gcgtttgaga aactgggctt catcgaccaa
     1861 cacacagtaa ggtttttctg gtaggcctcg gatatagcca aagatatccg ttgtttcctc
     1921 aatcgcaagg gcagggcgtt tcatgccaat tcgactcgac acatagccaa caccgtcacg
     1981 cgtatccaga gctgaagtca taatcacaac accttttcct tgctcctcat agttataggc
     2041 caccttgaga atctcgattg tctttccaga gttcatggtc ccataacgat aatacaactg
     2101 tgccatgttt cttgcttcac gtccatttct aaatttttgc tacattctag tatatcataa
     2161 ttttcttaag ctttaaacgg caaaatgtgg taaaatggaa gaaatcaaaa ctagtggagg
     2221 aagctattat gccatttgta cgcatcgatt tatttgaagg acgcacgctc gagcaaaaga
     2281 aagctcttgc taaggaagta acggaagcag ttgtccgcaa cactggagcc cctcaatctg
     2341 ctgtccatgt catcatcaac gacatgccag aaggaactta cttcccacaa ggggaaatgc
     2401 gtactaaata agctagctta agcagaattg cttaggcttt ttcaatctcc aagtagcatt
     2461 cattgaagaa atatcctaaa tttgttacaa tttgaaaaga aacttggaga atttccaaga
     2521 aaatagctat taattaaagg aaacattatg attacacgtg aatttgatac catcgctgct
     2581 atctctactc cactaggtga aggggctatt ggtattgtcc gcctgagcgg aacagacagt
     2641 tttgctattg cgcaaaagat ttttaaagga aaagacttga acaaggttgc cagccacact
     2701 ctcaactacg gtcacattat tgatcctctg actggtaaag tcatggacga ggttatggtt
     2761 ggggctatga agtctccaaa gaccttcact cgtgaggata ttatcgagat taacacccac
     2821 ggtggtattg cggtgaccaa tgagattctc cagctagcta tccgtgaagg agctcggttg
     2881 gcagaacctg gtgaatttac caaacgtgcc tttttaaacg gtcgcgtaga tttgacacag
     2941 gccgaggcgg tgatggatat catccgcgcc aagacagaca aggccatgaa catcgcggtc
     3001 aaacaacttg atggttccct ttctgatctt atcaataata tccgtcaaga aatccttaac
     3061 acacttgccc aaattgaggt caatatcgac tatcctgagt atgacgatgt tgaggaagcc
     3121 actactgctg ttgtccgaga gaagacaatg gagtttgagc aattactaac caagctcctt
     3181 aggacagcac gtcgtggtaa aatccttcgt gaaggaattt caacggctat cattggacgt
     3241 cccaacgttg ggaaatcaag ccttctcaac aacctcttgc gtgaggacaa ggctatcgta
     3301 accgatatcg ctgggacaac acgagatgtc atcgaagagt acgtcaacat caatggtgta
     3361 cctctcaaat tgattgatac agctggtatt cgtgaaacgg atgatatagt ggaacaaatc
     3421 ggtgttgagc gttcgaaaaa agccctcaag gaagccaact tggttctact agtgctaaat
     3481 gccagtgaac cactgactgc gcaagacaga caacttcttg aaattagcca agataccaat
     3541 cgcattattc tacttaataa aaccgacctg ccagaaacga ttgaaacttc gaaactacct
     3601 gaagacgtta tccgtatttc agtccttaaa aaccaaaaca tcgacaagat tgaagagcga
     3661 atcaacaacc tcttctttga aaatgctggc ttgattgagc aagatgctac ttacttgtca
     3721 aacgcccgtc acatttccct gattgaaaaa gcagttgaaa gcctacaagc cgttaatcaa
     3781 ggtcttgagc tggggatgcc agttgatttg cttcaagttg acttgactcg tacttgggaa
     3841 atcctcggag aaatcactgg ggatgctgct ccagatgaac tcatcaccca actctttagc
     3901 caattctgtt taggaaaata agaaaaatcc atgatccttc attcggtcat ggattttatt
     3961 gtctttatta gtaatctggt cttaagaccc ctgttacagt tgccttagtt gcttcgtagt
     4021 cgccatctac gacaaccttg ataatgcgtt tggcatcttc ttctggtgct ggaacaagag
     4081 gtagacgagt gggtccagct tcaaatccca tatagttaag aattgcctta actggagcag
     4141 gacttggata agagaagaga gcattaacct taggaatgaa tttacgctga attgctgcgg
     4201 ctttcttcat atcgctttct gcaatggcag taaacatctc gtgcatttca tccccatttg
     4261 tatgagaggc aacagaaata accccatccg ccccaaggtt catggcatgg aaagcatctc
     4321 catcctcacc tgtataaatc aagaactctt caggcttgtg ctcaatcaag taagccatat
     4381 tagccaagct agtacattct ttgacaccga taatatttgg atggtcagcc aagcgaagca
     4441 tggtttctgg agtcaattcg acaactacac gccctggaat gttatagata ataattggta
     4501 ggtcagaagc atctgcaata gtcttaaagt gctgatacat cccttcttga gaaggtttgt
     4561 tgtagtaagg aacaatagca agcccagctg cgaaaccacc aaattccgct acttctttga
     4621 caaactcaat agagtcacgc gtatcattag tacctacacc cgcaatcaaa ggaacgcgtc
     4681 cattgacaac cttttgtaca gccgcaaaca actccaactc ctcatcgtgg gtcaaagttg
     4741 gactctcagc agtcgttcct gcgagaagaa ttccatccgt atgatgggcc aataaatgct
     4801 caatcaaggc tggaatagca tcaaagttaa tagaaccatc ctcatgaaag ggggtaataa
     4861 aggctgtaat gattttacat tcttttaaat cttgataaga catgaaaaca cctctctatt
     4921 tcaaagaagg agttcatctg aactcctaaa cgatatgact attttaattc aaatttcaat
     4981 tcggctgttg gacgaaccaa tccacgttca tgaagagttt cagcaatctg aactgagttc
     5041 caagcagcac ctttgagaag gttatctgaa acaacccaca tgtgaattcc tttttctgca
     5101 tccaagtctt tacggatacg accaacaaag gtatcacgcg aaccaactgc attgatagct
     5161 tgaggataga tttgatgagc tacatcatct tcaagaacag cacctgggaa ggctgcgata
     5221 gctgctttta cttcttcgat tggagccact tcttttgttt cgatataaac agactcagag
     5281 tgagctgaca agactggaat acgcacacat gttgcagata ctgcaatgct atcatcttcc
     5341 ataattttct tagtttcctt ggtcatcttc atctcttcgt aagtgtaatc attgtcagtg
     5401 aagacatcga tttgtggaag agcattgaag gcgataggat aatgtttctt gtcaccgcct
     5461 gaaggtaaga tttccgcatg caaatcacgt ggtttcactc catcattcaa gacctcacga
     5521 agttcacgtt gtgtctcaag aattgctccc ataccagcac ctgaaacggc ttgataagtt
     5581 gaaacaatga tacggtccaa gccccatttt tggcgaaccg gctcaagagc caccatcatt
     5641 tggattgttg aacagttagg gcaggcaatg attccgttgt gggcatcaag tgcatgagca
     5701 ttgacctctg gaacaaccaa aggaacatct ggattttgac ggaaataaga tgtattatcc
     5761 actactacca cgccagcttt tactgcgtat ggtgcatact tagctgatgt cgaaccacct
     5821 gctgaaaaga gagcaatatc aactccttca aaagctgttt cagtcgtttc ttcaatcgta
     5881 atatcttgat ctttaaattt caatgactta cctgctgaac gtgcagaagc aaggtaacgg
     5941 attttatcga ttggaagtgt tgatttttcc aacattttta tcatctgagc accgacagca
     6001 cctgtcgcgc cgactacagc aactgtatat cccataaata acctctttcg gaattttcta
     6061 aaaatttcta taatagaagt attatactac tttttccatg aattgcaaag ctttttcatc
     6121 attttttgga aaataaaaat ccccaatcgc tagactaggg attaagaggc atcttcatgt
     6181 gatgagcagg ttcacacaac tcatcaaggt ccgctcctgc gttatgacct ccttatgctc
     6241 aatagtagcc cgaagactac ctatcgactc atcgcatatt ctatattact agagatagtt
     6301 gattttgtca ataagaaatt ttttcgatcg agtataacaa aaaactctcg tattcaagag
     6361 tttccctata catatcatgc taattgccgg gattgaaccg gcgacctcat ccttaccatg
     6421 gatgcgctct gccaactgag ctaaatcagc ttacttaaga agtatactat attcagaaaa
     6481 acttgtcaag tttccttaga aattttccat aggaaaaagc caggtaagaa ctacctagct
     6541 tatcttcttc tatttactta aatcaattcg acgaccaatt ttaccaataa taatcgcccc
     6601 aataatcaat gaagcaaatt caccgattcc tgttgtaaac caagtaagga agaatggtaa
     6661 ttctgctaca atatttaact ctgcagcgat agtaaacatg gaaattgaaa agaggattga
     6721 aaagaagaaa tgatcttttc gaatcaatcc gttaaagagg taatccttgc tatattttgc
     6781 aaaaagccaa acacctaaac tgaggaatac taaggttgaa ccaccaccaa caaagacatc
     6841 tagcagtccg aagctaaaga aattagcaat catacaaccg atggtaactc cgatgatgta
     6901 cttaggattg taaaaagcca taaagttcat catttctgaa atacgaaact gataagcacc
     6961 atagctgatg gcatttaggg gcggtgtgac agtcaaaacg acgtagatag cagcgacgat
     7021 tgcaatatct gcaacatcac gaatagttaa ttttttcatc ttttctcctt tggcggttgc
     7081 ccgcgtgtaa tatgcttggt gaaagaagct aagcaccaag ggttgcttta ttcaacccta
     7141 ctagtatagc acaatagcct gctttatgct atacttaaca catgaaaatt cctattcaaa
     7201 aattttttaa caatgaaatc ttagcctatc tcttttttgg tatagtgact actcttgttg
     7261 ctatactatt acgattagtc atttatcagc taagtcatca agaactcctt gctacggcct
     7321 tggcaaatat tatcggtatt ttatttgcct ttatcacaaa tgatacaatt gtatttaagc
     7381 aaaaaaagaa aaatagactt atccgtttag tgaaattttc tcttgctcgc ctttctactt
     7441 ttctgttaga cttgttttta acttttctct ttgtaaccca atttcctcac atcatagggc
     7501 aattcgtaac tgaaaacatt gataagataa atgctattga aacaataatt gcacaaatat
     7561 tcattattat catcaattat atcttgagca aggtctatat ttttagaaaa taatgctcca
     7621 tttatgagcc ttttaattgc cttctataat gatttaggat acaatgaaac acgaaacatt
     7681 gaaaggaaaa cagtatgtta aaaaatctaa aatcgttctt gcttcgagga aatgttattg
     7741 accttgctgt cggtgttgta attgcctctg cttttggtgc tatcgttact tcacttgtaa
     7801 acgacattat cactcctctt attttaaatc ccgctttgaa agctgctaaa gttgaacgta
     7861 tcgctcaact ttcttggcat ggagtcggct atggtaactt cttaagtgct attatcaatt
     7921 ttatctttgt gggtaccgcc ctcttcttta ttatcaaggg cattgaaaaa gcacagaaac
     7981 tgactggcat aaaggaagaa aaaactgccg aaaaaaaacc aaccgaattg gaagtccttc
     8041 aagaaataaa agctctcctt gagaaaaaat aatcgattaa aaggatctag cgcaaagcta
     8101 aatccttttt tctttactct ttgggtaact tgcctgcttt ttcaagcatt tttttgataa
     8161 gataaggcat cttaacattc tcccgccctt ttttctcaat cagttttttc acaaattctg
     8221 gcatttgtaa atcttcagat tcagccaaaa tgttttttgt ctcgtctgaa ggaactaact
     8281 catcaaaggt ctcttcttct gggagaaatt ccttaaactc ttgattttca ttgactctaa
     8341 ctgtattgac caaagcatca atcaaacgag aatctgtatt tggcattggt ggacgatggt
     8401 agtttacccc aaattcctga cataaatcat aacattccac atcattgtca aacaagacct
     8461 caatatgctc actgatgaaa ctaataggta caaaaatata atggtctgga tgttctgtct
     8521 gttctctgag atactccaaa acatctggtt taatccatgg aataccaata tcactctcac
     8581 tctgccaagt gttagtatat tgttttgaac tcaagcccag tttttcagcg actagcttgc
     8641 tattttcgaa aatttggtct atataaggat caccaaagtc caaggcaaaa ataggcacac
     8701 tatgggctga aaagatggct ttaaagctgt cttgctttac ttcttctttt aaaatcttag
     8761 caatttcatc tgcccaatag tttaagagcg cttcttcctg ataccagtcc ttaatgacca
     8821 aaaattgaat ttgtttgctt tctaaaaatt tttcatagcc catgacagag tagaaagaat
     8881 aatggggctc caaaatcaag caaatacact gttcaattcc gtctgcttcc atttgaccga
     8941 tcacatctgg aataaaggga ctggaaaact tattagcaaa gtagacactg tattcattcc
     9001 ccagcctagc ttctaccaag gcaacctctt gacgggtaat tttttgcaga ggcgtgcctc
     9061 caattcgtac ataattatca tagagagtct gaatctcgtg gtcttggggt ctcaccccac
     9121 gacgaatatt tgtgaaaaaa tcagctacac cttcaaaggt aatctcttct ggtgaaccga
     9181 aggtcatcat taaaattgct tttttcatga ctgcttcctt gtgatgtttt tatcaagttt
     9241 attttatcat taattcatcg ataagtaaac gctagcatag ggacattctc aatcttctgt
     9301 cttttgtttt tctcttcttt ctatgataca atggaaaaaa taaattcaaa aggagttttt
     9361 ttatgactta tccaaatctc ttggaccgct tcttaaccta cgttaaggtc aacacgcgct
     9421 ctgatgaaca ctctactact actccaagta cacagagtca ggttgacttc gcaacaaatg
     9481 tcctaattcc tgaaatgaaa cgtgttggac tgcaaaatgt ttactatcta ccgaatggtt
     9541 ttgctattgg aaccttgcca gccaacgatc cgtctttaac acgtaagatt ggttttatat
     9601 cgcacatgga tactgctgat tttaatgctg aaggagtcaa tccacaggta attgaaaact
     9661 acgatggtgg tgtgattgaa ctagggaatt ctggtttcaa actcgatcca gctgacttca
     9721 agagtcttga aaaatatcca ggacaaacgc tcatcacaac agatggaaca accttgctag
     9781 gtgctgatga caagtcagga attgctgaaa ttatgacagc cattgaatat ctaactgctc
     9841 atcctgaaat taagcactgt gagattcgtg ttggttttgg tccagatgaa gaaatcggtg
     9901 ttggtgccaa taaatttgat gcagaagatt ttgatgtgga ttttgcctac actgttgatg
     9961 gtggtccact aggtgaactt cagtacgaga ctttctcagc cgctggtgct gaattgcatt
    10021 tccaaggccg taatgtccac cctggtactg ccaaagggca gatggtcaat gcccttcagc
    10081 tagcaattga ttttcataat caacttccag aaaatgaccg acctgagtta actgaaggtt
    10141 accaaggttt ttaccatcta atggatgtga caggtagtgt tgaggaggcg cgtgcaagct
    10201 acatcattcg tgattttgaa aaagatgcct ttgaagcgcg taaagcatcc atgcaatcta
    10261 tcgctgataa gatgaatgaa gaacttggga gcgaccgtgt cactctcaac ttgacagacc
    10321 agtactacaa tatgaaagaa gtcattgaaa aagatatgac tccaattacc attgctaaag
    10381 ccgttatgga agatctaggt atcacgccta ttatcgaatc aatccggggt ggaacagacg
    10441 gctctaagat ttcctttatg ggaatcccaa ctccgaatat ctttgcaggt ggcgaaaata
    10501 tgcacggacg ttttgaatac gttagccttc agactatgga acgtgcagtt gataccatca
    10561 ttggcatcgt agcttataaa ggctaaaaag acgaggtggc tcagctactt cgcctttctt
    10621 tttattctac tggtttttct tgatttccag tagttgtaga agattctgtt gtttcatttt
    10681 ctgaagttga ttcagcaggt ttagaatctc ttgtattgct tggtttgttt tcgtcgctag
    10741 cagtttcaat gttagattct gcagttgcgt ttggttggtt ctcagcactg gtgttatcac
    10801 catttgcttc agcatttctt gctggacttg tttcttcact tgcgctagct tttgactgga
    10861 tttgatgatt caaaactaga atagcttttg tcggttcaag taaagctgtt ttgtctttac
    10921 tcttagcaga aagttgatct aataatgcat ccaccttatc aaagtccgca tcagatccat
    10981 tattactttc taaataagag tgaagcgaca tgagaatatc gtagagtttt tgatagagta
    11041 caagtgtctg aggatcttgc tcagcatttt ccttttcttg ttgaagggcg ctagcgatac
    11101 gagtcaaaac atcttttacc tgactgttta cttcatccaa gtctgcatca gccttgtttg
    11161 tggcagcttt tagattttct acttcttctg ccaaggattg tctgattcct tcttcatgga
    11221 tttgttccaa gagttgattt gccttgctca aaagactttc tacttcttcc ttgctatctg
    11281 tcgcagatta ttggttgcca tctaccatgt actcctaaaa caggagagtt ataatccaag
    11341 attacaaggc cttacagaaa taagaaatcc agataagaca atgttcgtcc aagacgctat
    11401 tcgcttcgca cagcagcacg gattcaatat gctttaattt taaagtttag gtgtcaagac
    11461 ctctttttag tgtgcccaaa atttagagaa gtaatcaatc aactaacttt tatttttttc
    11521 aaactttcag taaactgacc taaagctaac tcaatctgtc tttgttcgat aggcttgtct
    11581 ttgtagatgc ttctgctatc agctagaagt tgatctactt ttgccaagac tgccttctca
    11641 taaaaagttc caggttgata gttggattgc agggatggaa tcttgttttt caaagccgct
    11701 tcatatccct tagtttgaac cttgatgtag tgattgtggt cgccacgagg aatcacaaaa
    11761 ccttctgaat cttcacttat aattcgattg gcatcaaaac catgaccatc ttcttcctca
    11821 tggtggacat gtagtgacgg attacttaat acagaactag aagaacttcc taccttttcc
    11881 gtgttagagt gtgatggggg attgttaaga gatgacttag gaatatagtg atagtgaccc
    11941 catgtcttac tatataagca tcacctgtat ctctgacaat atcattaggg ttaaagacat
    12001 atgtggctgc taattcacct gccgacaagt cactctcagg aatgaaatga tagtgaccac
    12061 catgtggtac tatagtaggt tgaaatagaa tatgagcaaa ttgataaggg gattttaaag
    12121 taatttctaa caatgattta gaaactatga tgtgctattc taaattcaac tcactatata
    12181 taaccatcat cggtagtata acgtccctgt aattttgcta cagatacttc tgcactagct
    12241 cctttatcgt ctttaccatg ttcttgtttt tggcgattga tttcatcttt tgttcgtaca
    12301 ttttctgcat gagcttgatc tttaaggtaa acataatact ttccatctac cttaataata
    12361 tatcctccct taacctaact gacaatatct tgatctttcg gctgatagtt gggggctttc
    12421 attaatggct cttcactaaa gagcgcatca aaaggaactt taccattata gcagtgataa
    12481 tgatcgccat gagaagttac ataaccttga cctgtaatct taataacaat ttgttttgct
    12541 tgaattcctt ctttttgact aacctagtct ggagtcaaat tttcagtctt cttagtgtct
    12601 ttattactgt ttacatatga aacacgattt ttatctgtat tggcctgtta gctatgttgg
    12661 ttcagagcat aaacacacag acttaaggaa aggataacaa cagatccagc tgctatatat
    12721 ttctttttaa atttcataat tacctcattt ct
//
LOCUS       DAVKGU010000054        12485 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKGU010000054 DAVKGU010000000
VERSION     DAVKGU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.054"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            481..774
                     /locus_tag="VW077_001410"
                     /pseudo
     CDS             481..774
                     /locus_tag="VW077_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            881..1232
                     /locus_tag="VW077_001411"
                     /pseudo
     CDS             881..1232
                     /locus_tag="VW077_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1508..2814
                     /locus_tag="VW077_001412"
                     /pseudo
     CDS             1508..2814
                     /locus_tag="VW077_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2854..3864)
                     /gene="tsaD"
                     /locus_tag="VW077_001413"
     CDS             complement(2854..3864)
                     /gene="tsaD"
                     /locus_tag="VW077_001413"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9455416.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAATYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3854..4291)
                     /gene="rimI"
                     /locus_tag="VW077_001414"
     CDS             complement(3854..4291)
                     /gene="rimI"
                     /locus_tag="VW077_001414"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9455417.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYVLAYDGAEVIGFLAVQENIFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4288..4971)
                     /gene="tsaB"
                     /locus_tag="VW077_001415"
     CDS             complement(4288..4971)
                     /gene="tsaB"
                     /locus_tag="VW077_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9455418.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVAPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5149..5334
                     /gene="cibA"
                     /locus_tag="VW077_001416"
     CDS             5149..5334
                     /gene="cibA"
                     /locus_tag="VW077_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9455419.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5336..5488
                     /gene="cibB"
                     /locus_tag="VW077_001417"
     CDS             5336..5488
                     /gene="cibB"
                     /locus_tag="VW077_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9455420.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            6306..6539
                     /locus_tag="VW077_001418"
     CDS             6306..6539
                     /locus_tag="VW077_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9455421.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6541..8220
                     /locus_tag="VW077_001419"
     CDS             6541..8220
                     /locus_tag="VW077_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9455422.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8321..8410)
                     /locus_tag="VW077_001420"
                     /pseudo
     CDS             complement(8321..8410)
                     /locus_tag="VW077_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8599..10512)
                     /gene="mnmG"
                     /locus_tag="VW077_001421"
     CDS             complement(8599..10512)
                     /gene="mnmG"
                     /locus_tag="VW077_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9455423.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMILPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQDKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10522..10977)
                     /locus_tag="VW077_001422"
     CDS             complement(10522..10977)
                     /locus_tag="VW077_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9455424.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(11118..12239)
                     /gene="mnmA"
                     /locus_tag="VW077_001423"
     CDS             complement(11118..12239)
                     /gene="mnmA"
                     /locus_tag="VW077_001423"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9455425.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3539 a         2922 c         2464 g         3560 t
ORIGIN      
        1 caatatctaa atttttataa tcatcaataa cctttgaatc tctagatacg attatcaaaa
       61 taggatatag agacatcctt ctgatctgct ttttacaaag tccaattata tgcggatcta
      121 tacctccaca atgtccatta ttatacctaa ctataatatg agccgaaaac actatatcct
      181 taatgtctcc atatccatca gggatattaa tatttatttt tccacaacta tattgcattg
      241 taaccatctc cttaaacgac gcattatgat atttgataga gaaattttta tgaataactc
      301 aataatttta tagtaaatca tgcttatatc tcaaagatac ctattttctc ttgtctcgac
      361 cttctccaaa gaattgctat aatactatta caaatacatc tgcactacac ttcaaatttt
      421 agcactgtat aaaaacgttt caacacacta actttaagaa aacttccact attaattgaa
      481 aaagttgata gagataaatt aaagatctat attgaaactc atcccgatgc ttatttgact
      541 gaaatagctg ctgaattcaa ctgtcctcca acaactattc attacgctct aaaggctatg
      601 ggatatagtc taaaaaagag ctgtacctac tgcgaacaag acccagaaaa agtaaatcgg
      661 ttccttaaag aattgaatca cttaagctac ctgactccta tttatattta tgagacaggg
      721 gttgagacct atttttatct cgaatatgat cgagccttga gcaggcagtt agtctctctg
      781 gaagaagata taattatttg aattaagatc gagacaacgc acaccagaga ttgcgatact
      841 gttatagaag tactaatgcc cttttttgtt tcaatatact atggctccga tgacctataa
      901 agatacgatg acgagtgact ttttcgaagc ttgcttccaa aaattcttac tacctacttt
      961 agatacacca tcccttatca ttatggacaa tgcaaggttt cacagaatga acatgtgtaa
     1021 ggagcagggc atagactgtt accacttcct acctattcac ccgagtataa tcccattgag
     1081 aaaatatggg cttacatcaa aaacatctca gaataatatt gtcaaattac gatgcttttc
     1141 ttgaggcact tttgtcctat tcttgtttca gccgactata ctccgttatt gggcagctac
     1201 ggaacagtcg atgggacgat ggggggacat aaaaaaatcc tccagttttg ttttttataa
     1261 cagtatactg gagaattgac aatctcggta gatacctcgt tatagcgcgg ttacttatta
     1321 ggcagttaca aaacaactgt gaacagaaaa cattccagag tcagacaaga ctttggaatg
     1381 ttttggctct ataatttctg tagtgggtaa tcccacccca ggaattatag ggtcgtttct
     1441 tgtagaaaaa aagccccata tgacctataa tgaaaagcgt ctaaccaact cattagaaag
     1501 ggttcatatg gaacaactta agaataccac agatttgctc ggattgaaag acaaaaatat
     1561 caaaatcttg tctgttctga aataccaaac ccatctagtc gttcaggcaa agttggattc
     1621 ccccgctcct ccttgtcctc attgtcaagg gaagatgatc aaatacgact tccagaaagc
     1681 ctctaaaatt ccgcttctcg actgtcaggg tttacccacg gtactacatc tcaaaaagcg
     1741 ccgctttcag tgcaagaatt gccttaaggt ggtcgtttct caaacatcca ttgtcaagaa
     1801 aaattgccag atttccaaca tggtgagaca aaaaatcgct cagctcctcc ttgaaaagca
     1861 gtctatgact gagattgccc acagattggc ggtctcaact tccaccgtca tccgaaaact
     1921 gagggaattt aagtttgaaa ccgattggac caagttgcca aaagttatga gttgggatga
     1981 gtatagcttc aaaaagagca aaatgagctt cattgcccaa gattttgagt ccaaatccat
     2041 cctcgcaatt ttagacgggc gaactcatgc ggtgattcga aaccatttcc aacgctatca
     2101 gagagaggtt cgggagctgg tcgaggtcat caccatggac atgtacagcc cttattatcg
     2161 gctcgctaag caactctttc caaaggcgaa gattgttctt gaccgcttcc acattgtcca
     2221 acatctgagc cgagctatga accgagtacg aatccaaatc atgaaccaat ttgaccgaaa
     2281 atccttggag tatcgggcgc tcaagcgctt ttggaaccct cgctttttcg tttctaggct
     2341 cgggctaaat cagtccactg gactgattta ctacaccagg atagcttcaa gctctgtcag
     2401 aaacgattct atcagcccac gtttcgaatg cacttaaccc atcgggaagt acgagataag
     2461 ctgctttctt actctgaggg attacaggtt cactacgaac tctatcaact cctgctcttt
     2521 cattttcaag agaagaatgc cgaccatttc tttggattga ttgagcaaga actgccaacg
     2581 gttcatccgc tttttcaaac ggtcttttgg acttttttaa gggatagaga taagattatc
     2641 aacgcactta agctgcctta ttccaacgct aaacttgaag cgaccaataa tttgattaag
     2701 gttatcaagc gcaaagcctt tggtttccgg aactttaaca attttaaaaa acggattttg
     2761 atgactttga acatcaaaaa agagagtacg aatttcgtac tctccagatt gtagcttttc
     2821 gcctacccac tacacttgac aaagagccac tctttattcc atggtatcaa aggcaagact
     2881 tggtttggca ttgaggtccc agcctgcgaa gttttctttg ttccactcgc tgacgctggc
     2941 ataggcaatc atacctgcat tgtctccgca gagtcgcaga ggggggatga taaccttgac
     3001 atctgtgatt tcggctgcta ggcgttctct gagaccttta ttggctgcca caccacctgc
     3061 cacaactagg gttttaacag gatatttctc caaagccttc ttggtttttg ccatgagaat
     3121 gtccataact gctgcttgga aggaagcaca caaatcttct gtagacaggc tttctccctt
     3181 ttgctcggca ttgtgatgaa gattgataaa ggcagatttc aaacctgaga aggagaactc
     3241 cagattatct tccttaatca tggcacgggg gaaatcataa atatcctgcc cctgatgagc
     3301 cagctcgtca atctcacgac ctgcaggata ggtcaagccc atgacacgac cgaccttgtc
     3361 ataagcctcc ccaactgcat cgtctcgtgt ctccccaaca atcttgtaat ctcctgcctc
     3421 cgaaacatag accaactctg tgtgcccacc gctaaccaag agggctagca agggaaactc
     3481 caaaggctcc acactctgag ctgccatgag gtgcccagcc atgtgattaa caggaatcag
     3541 tggaagtccg tgagcccaag caaaggcctt ggcagctgac aaaccaacta gcaaggctcc
     3601 gaccaagcct ggtccgtagg tagccgcaac agctgtcacg tcctcttcgg taatccctgc
     3661 ttctgccaat gcctcctcga tacaggctgt aatgacctcg acatggtgac gactggctac
     3721 ttcgggcact acgccaccaa aacgtttgtg actctcaatt tgactagcaa tgacattgga
     3781 caagagctca tcgtcgtttt tcaagacggc gacactggtc tcatcacagg atgtctcaaa
     3841 tgctaaaata tatctatcct tcatctattt ctctcttcat gataatggcg tcctcgactg
     3901 ggtcatggta gtaggccttt cgctcagcga tgaccgccat tttttctttc ttgtaaaatg
     3961 cttgcgctcg ttgatttgac tttctgactt cgaggaaaat ctccttatct gtcggcaatt
     4021 gagcaaacaa ggctgacgca attccctgac cctgataagc tcctttaaca gcgatttgca
     4081 agacttctgc ctcaaagata ttttcctgaa cagctagaaa accaatcact tctgccccat
     4141 cataagccag aacataccaa gtctggtctt gggacaggtc tgcttggatt tgctccagag
     4201 tccaaggact gactaggtaa acagctgcca taacagcgta gatggcttga gctaggtcag
     4261 gctgttgttg aattcgcttg atttctatca taggcgttta atgtaggact caccagactc
     4321 ggtatggttc ttgagccagt tttcctcagc ctcgactcgt ttgaggtaat tcggcacaaa
     4381 atcatgcaag gagtctgctt ccttgtccca agctaaaaga gcaagattgg ctgcattagg
     4441 caaggtttct ttataattag tccttggcaa gtgttcttga atctgctcca caaagggggc
     4501 aacttctccg acaaaggtta cctgactagt acccttgact ttttctagca cctcttcaaa
     4561 agataggtgc gcttctgcca tgacaggttt ggcattttca taaaatcctg cataaacatt
     4621 attgcgacgc gcatccatca aggggacaaa caaaccttct tgttggtggg gcaccagagc
     4681 caagagactc gacataccaa ccaactcgat gttcagggtg taagctaagg tcttagcagt
     4741 tgctaccgca attcgcaagc ctgtatagct acccggccct tcagctacca cgattcggtc
     4801 caaatccttt ggtgtccaat ccaaacttgc catcaaaaaa tcgatggcag gcataagagt
     4861 aatactgtga tttttcttaa tattaatcgt cgtctcggca agaacctgct tatcctctaa
     4921 aatagccaga gaaagagcct tgctggacgt atcaaaagct aatactttca taacacattc
     4981 ctatcttttt gtctgcttac tattatacta caaaagctgg cacatgggaa ttttctttgc
     5041 ccccagacaa gagtgccctc acttaactaa aaataattta aaaaaatgct cacttttcct
     5101 tttcttttcc gaatataaaa gtgaacaaga aaaaaggagg aaagttcaat gacaaatttt
     5161 gacattcttg acaatcaatt tttatcctta tctgaaaatg aattatcaga tattgatggc
     5221 ggtctcgctc ccttggttat ctttggagta gcagtatctt ggaaggctat tgcaggtgga
     5281 acagcactta taggttctgg tttggcagct ggttattttt taggaggaga ttaatatgat
     5341 gaaaaatttg aacaactatc gtgaaatttc taataaggaa ttgcaagaaa tcaagggtgg
     5401 ctttggtgta ggtgttggta tcgctttatt tatggcaggt tataccattg gaaaagacct
     5461 tcgtaaaaag tttggtaagt catgctagat aagaaacaca tttttagaag gataaatttt
     5521 attgtcttca tctcttacag tttgctcagc attctcaatg atttgaacat tactactatc
     5581 cctttaccat tcgatttatc tgtttgtatt gttttatttt tatgcttcaa ctctattttt
     5641 gagcagaaca atgactccca taaaaataat aagctttgaa aattccattg tcatgtcatt
     5701 ttagaaaaat gcaaagacca cctcatcttg atagatgggg tggaattttc gtgtcgtaaa
     5761 tctactatct ctacatcccc aaacaaaaaa ccccagcata agcagggcat ctaagcattt
     5821 aatccaaagt aaaatacaaa ccaaacgaca tagggcacga ggaggagaaa aagcgagtag
     5881 agagtcacaa aggtaatttt ccacaagaac ttggtttgtc atcgttccag tttggcaaat
     5941 ggaagatttc ctccataaac gcaagcaaca aaaacgataa aagctaacaa acgaactccg
     6001 atagcaaaag caaataagtt atacataggg caacctcctt gacttaaaat ctatatggaa
     6061 ttatgacaag caataaattt cacttccgtt atcaacataa tacattttct ttatttttgt
     6121 aaacgcttac caaagaaatt gccccctaac tttctcgttt ccgtctttta ctaatttttc
     6181 attttgtggt ataattgaaa taattgtaac gaatcaaggt caatctagac acaaaatgga
     6241 atgaaatcaa gcaaatatct gctaaaagtt tggaataagc tgacctgtaa atagaaagga
     6301 actatatgat ttacaaagtt ttttatcaag aaacaaaaga acgtagccca cgccgtgaaa
     6361 caacacgcgc gctttaccta gacatcgatg ccagctcaga acttgagggc cgtatcactg
     6421 ctcgccaact tgtcgaagaa aatcgcccag agtacaatat cgaatatatc gaactcttgt
     6481 ctgacaaatt gctcgattac gaaaaagaaa ctggcgcctt cgaaattacg gagttctaat
     6541 atggcctaca ctcttaaacc tgaagaagtc ggcgtttttg ccatcggtgg tctaggagaa
     6601 atcgggaaaa acacttacgg aattgaatac caagacgaga ttatcatcgt cgatgctggg
     6661 attaaattcc cagaagatga cttgcttggt atcgactatg tcattcctga ctactcttac
     6721 atcgtggaca atatcgaccg cgtcaaggct gttttaatca cacacggaca cgaggaccac
     6781 attggtggga ttccgttcct actcaagcaa gcaaatgtcc ctatctacgc tggaccgctt
     6841 gccttggctt tgatccgtgg gaaactcgaa gaacacggcc tcttgcgcaa cgccaaactt
     6901 tacgaaatca accacaacac cgagttgacc tttaaaaatc tcaaggcaac tttctttaga
     6961 acgactcact ctattccaga gcctttgggg attgtcattc atactcctca agggaaaatc
     7021 gtctgtacgg gggactttaa gttcgacttt actccagttg gagaacctgc ggacttgcat
     7081 cgtatggctg cgcttggtga agaaggtgtg ctctgtctcc tgtctgactc gacaaatgcg
     7141 gaagtaccaa cctttaccaa ctctgaaaaa gtcgttggtc agtccattat gaagattatc
     7201 caaggtattg aaggacgtat catctttgca tcctttgcct caaatatctt ccgtctccag
     7261 caggcaacag aagctgctgt taagactgga cgcaagattg cggtctttgg tcgttctatg
     7321 gaaaaggcca ttgtcaacgg aatcgatctt ggctacatca aagctcctaa gggaaccttt
     7381 atcgagccaa atgaaatcaa agattatcct gcaggagaag ttcttatcct ctgtacaggt
     7441 agtcagggtg agcctatggc agccctctct cgtatcgcca acggaaccca ccgtcaagta
     7501 caattacaac caggtgatac cgttatcttc tcttctagtc ccatccctgg aaacactact
     7561 agcgtcaaca agctgattaa catcatttct gaagctggtg tcgaagttat ccacggtaaa
     7621 gtgaacaata tccatacatc tggacacggt ggtcagcaag agcaaaaact catgctctgc
     7681 ttgattaagc caaaatactt catgcctgtc cacggtgaat accgcatgca aaaagtccac
     7741 gctggactag cagtggatac tggtgttgag aaggacaata tctttatcat gagcaatggc
     7801 gatgtgcttg cccttactgc tgactcagct cgtatcgcag gtcatttcaa cgcccaagat
     7861 atctatgtcg atggaaatcg tatcggtgaa attggcgcag ctgtcctcaa agatcgtcgc
     7921 gatctatctg aagacggtgt cgttctggca gtcgcaactg ttgacttcaa atcgcagatg
     7981 attctatctg gtccagacat cctcagccga ggctttgtct acatgagaga gtctggcgac
     8041 ttgattcgcc aaagccagcg tatcctcttc aatgccattc gtatcgcact gaaaaataag
     8101 gatgctagcg tgcaatctgt caatggtgcc attgtcaacg ctattcgccc cttcctctat
     8161 gaaaataccg aacgtgaacc gatcatcatc ccgatgatcc tcacaccaga tgaagaataa
     8221 tactcttcaa accacgtcag cttcgccttg ccgtatatat gttactgact tcgtcagttc
     8281 tatccacaac ctcaaaacgg tgttttgagc tgacttcgtc agttctatct acaacctcaa
     8341 agcagtgctt tgagcaacct acggctagct tcctagtttg ctctttgatt ttcattgagt
     8401 acaagtcaac aaaacagccc cgtcttcgga gctgtttttc tctatgcttt cttttgagat
     8461 taaaactcaa tgaaaatcag accagaaggc taggaaaatg cataacttga gttagctccc
     8521 atagttcggg aaactatggg aggctggaga tgaatcaaag ccaagctttg aactcattcg
     8581 taagaagccg acgacgtatc attttgattt ttgaagagtt ttagaaatac tacgattttt
     8641 accttccaga tacaccatca aaatagaaat atctgctggg tttacccccg aaatacggct
     8701 ggcttggccg atggtttctg gattgatgag tttgaacttc tgacgagctt ccgtcgcaat
     8761 agaatcgatg tcatcccagt caatattggc tggaatgcgt ttttcttcca tacgtttcat
     8821 cttggcaacc tgatccatgg cttttgaaat atagccttcg tacttgattt ctgtttcaat
     8881 caattcgata atcttatcat ccaaatcctc tgcagctggt ccgatgaagg ctaccacatc
     8941 ttggtaagaa acttctggac ggcgaaggaa ttccttggct gtcactgcat ctgtcaaggg
     9001 tttgaagccc atctcctcaa ccttagcatt ggtttccttg actggcttga gtttgatact
     9061 gtctaggcgt ttcatctcat tatcaaattg atttttcttg atttcaaaac gagcccagcg
     9121 ttcatcatcc acaaggccaa tctcgcgtcc catctcagtc aagcgcatat cagcattgtc
     9181 atgacggaga atgagacggt attcagcacg actggtcaag agacggtagg gttcgatggt
     9241 tcccttggtc accaaatcat caatcatgac cccaatgtaa ccgtcactac gcttcaaaat
     9301 caactcaggc ttatcttgga ttttcagagc cgcattgatc cctgcgataa tcccttggcc
     9361 tgctgcctct tcgtaacctg atgttccatt tgtctgacca gcagtgaaaa gtcctgagat
     9421 tttcttggtt tccaaagtcg cacgcaactg gtgaggcaag atcatgtcat actcaatagc
     9481 ataacctgtt cgcatcatct ctgcattttc cagacctttg atggaatgaa ccaagtcacg
     9541 ttggacatcc tcaggcagac tggttgaaag tccttggaca tagacctcct ctgtattgcg
     9601 tccttctggc tcaaggaaga gttggtgacg ttccttgtcc gcaaagcgca caatcttgtc
     9661 ctcaatcgat ggacagtaac gaggtcccac tcccttgacc acacctgtaa acataggcgc
     9721 acggtggagg ttgttttgga taatctcatg actggtacca ttggtgtagg tcaaccagca
     9781 tggcacttgg tccttgacat aatcctcatc acgtgaagtg tatgagaagt gattaggtgc
     9841 ttcatctcct ggttggattt ctgtcacatc gtaattgata gaagaagcct tgacacgtgg
     9901 aggggttcct gtcttgaaac gaccgatttc gaggcccagt tccttgagat tgtcagctag
     9961 gttaatagaa gccaagctgt ggttaggacc tgatgagtac ttgaggtctc cgatgataat
    10021 ttccccacgg agagcagtcc ctgtcgtcac aataacagcc ttagcagcat attcttgatg
    10081 ggtggctgta cgcacaccga caaccttgcc atcttccacc aaaatctcat caatcatggt
    10141 ttgacgaagg gtcagatttt cttggttttc aaccgtcttg cgcatctcct tagagtaaag
    10201 ttccttgtca gcctgcgcac gaagggcacg gacagctggc cccttccctg tgtttagcat
    10261 cttcatctgg atgtaagtct tgtcaatggt tttggccatc tcgccaccga gggcatcgac
    10321 ttcacgcacg acaatcccct tggcagaacc accgatagag ggattacaag gcatgaaagc
    10381 cagcatttca atattgatgg tcgcaagcag gaccttacag cccatacggc tagcggccaa
    10441 ggaagcctca accccagcgt gtcccgcacc aattacaata atatcgtatt cttcagtaaa
    10501 atgataagtc atgtttctct cctattcctc aagatgaatg tgtcttagtt ggccttccca
    10561 atctggtagg gccgttttta aaaaggctgg aactagctgg atatcctcaa gcttatccaa
    10621 gtcaatccac tcacagggct gccttttctc atcttcctgc atggtcaacg gggcatcttc
    10681 aagcaaatcc accagataat gaaactcgat attgtgatag gaaacgccgt ccacttcaaa
    10741 acgattttca accacaaaag ctagctgccc agcttgagat ttaacaccca gttcttcctt
    10801 cacttcacgg actaccgcgt cttccgtttt ttcgttaact tgaatcgcac cgccgataat
    10861 gtaatacttg cccttgtctt tggtgactag aagcttgtga ttttggacaa tcaaagctgt
    10921 cgcccgaaca ccaaaaaccg tattgtctac ttttgtccga aagtcttgtt gagtcattct
    10981 tgtcctttcc cttaaacgac acaaaaacag tcaaaactac aaagaagtgc aggacaaaaa
    11041 agcctgcaac atccatgagc tttgaccatc atttctattg ctgttattat tgtagcaaat
    11101 tgagaaaatt tgtcaatcta aatgtactga caaacttgtc catcgcggta agcattgtca
    11161 atcaaaccgc cacctagaca ctcttcgcca tcgtaaaaga caactgcctg tcctggtgta
    11221 atcgcgcgtt gtggttccgc aaagataact tctgccttgt ctcctttgac atggacggtc
    11281 accttagagt caggctgacg gtaacggaat ttagccgtac attctagcgt aaactcttct
    11341 ggcatttcac gagtaaagtg gacttgactg gcttctaggc tagttgacat gagcgaatca
    11401 tggtagaatc cttgtcctac atagagaata ttcttgctta gatcttttcc gacaacgaac
    11461 caaggggcat tgtcaccgcc gtgttgccca ccgataccga gtccgccacg ctgaccgatt
    11521 gtatagtaca taagacctgc atgctcgccc atatcgcgac catccacagt catcatgcga
    11581 ccaggctgag ctggcaggta gttgctgaga aagtttttaa agttcttttc tccgataaag
    11641 caaatccctg tcgagtcttt cttcttagca gtcgaaaggc ctgcttcttc tgctagtttt
    11701 cgaacttcag gcttttccaa atgtcctagt gggaacatgg ttttttgaag ttgttcttgc
    11761 gaaagttggc tgaggaaata ggtctggtcc ttgccattgt ccacgccacg aagcatgtga
    11821 acagtgccgt cctcatcacg cgccactcga gcataatgcc cagtcgctac atagtctgcc
    11881 cccaaggtta tggcatagtc caaaaaggcc ttgaacttga tttccttgtt gcacataacg
    11941 tccggatttg gcgtgcgccc tgcacggtat tccgctagga aatactcaaa aacgcggtcc
    12001 cagtactctt tttcaaaatt gacagagtag tagggaatgc caatctggtc tgccaccgca
    12061 accacatcct tgtaatcttc ggtcgccgta cagacgccgt tttcatctgt gtcatcccag
    12121 ttcttcatga agataccgat cacatcgtag ccctgctcct tgagcaaaag agccgtcacc
    12181 gacgaatcaa caccaccact catccccacg acaacacgtg ttttagagtt atcactcatg
    12241 gtaagtctcc catctattcg tttattcacg attgaaggtc gtgtgtgctt cacgattgaa
    12301 ggtcgcttga gcagtattca ttataacacg cttggttaga gaagacaagg aagagcttaa
    12361 aaattgtttt ttaaaactta tagtcaatat acagattatg tgaatgatgt gagtatacct
    12421 tcttcttatc ttcattcaaa taactataat aaataaaaca acccattttc tctaatctat
    12481 ttttc
//
LOCUS       DAVKGU010000055        12262 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKGU010000055 DAVKGU010000000
VERSION     DAVKGU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.055"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..350
                     /locus_tag="VW077_001424"
     CDS             <1..350
                     /locus_tag="VW077_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9455426.1"
                     /translation="KLLSYSQNLKHHYQLLLFHFQNKEPEKLFGLIEDNLKQVHPIFQ
                     TVFKTFFKDKEKIINALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALN
                     IKKERTKFVLSRA"
     gene            complement(434..1636)
                     /gene="rpsA"
                     /locus_tag="VW077_001425"
     CDS             complement(434..1636)
                     /gene="rpsA"
                     /locus_tag="VW077_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9455427.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1798..1869)
                     /locus_tag="VW077_001426"
     tRNA            complement(1798..1869)
                     /locus_tag="VW077_001426"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1835..1837),aa:Gln,seq:ttg)
     gene            complement(1879..1959)
                     /locus_tag="VW077_001427"
     tRNA            complement(1879..1959)
                     /locus_tag="VW077_001427"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1923..1925),aa:Tyr,seq:gta)
     gene            complement(2009..2239)
                     /locus_tag="VW077_001428"
     CDS             complement(2009..2239)
                     /locus_tag="VW077_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9455428.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2407..3051)
                     /gene="pcp"
                     /locus_tag="VW077_001429"
     CDS             complement(2407..3051)
                     /gene="pcp"
                     /locus_tag="VW077_001429"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9455429.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(3066..3989)
                     /locus_tag="VW077_001430"
     CDS             complement(3066..3989)
                     /locus_tag="VW077_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9455430.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3986..4675)
                     /locus_tag="VW077_001431"
     CDS             complement(3986..4675)
                     /locus_tag="VW077_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9455431.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4740..5444)
                     /locus_tag="VW077_001432"
     CDS             complement(4740..5444)
                     /locus_tag="VW077_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9455432.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKCGNVAPSQKDYDLNSTGWAPSYQDPASYLNIIDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDILTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            complement(5500..6522)
                     /locus_tag="VW077_001433"
     CDS             complement(5500..6522)
                     /locus_tag="VW077_001433"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9455433.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6654..9125)
                     /gene="parC"
                     /locus_tag="VW077_001434"
     CDS             complement(6654..9125)
                     /gene="parC"
                     /locus_tag="VW077_001434"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9455434.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYNAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(9545..11488)
                     /gene="parE"
                     /locus_tag="VW077_001435"
     CDS             complement(9545..11488)
                     /gene="parE"
                     /locus_tag="VW077_001435"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9455435.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11624..>12262
                     /gene="plsY"
                     /locus_tag="VW077_001436"
     CDS             11624..>12262
                     /gene="plsY"
                     /locus_tag="VW077_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9455436.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
BASE COUNT         3544 a         2778 c         2151 g         3789 t
ORIGIN      
        1 acaagctttt gagctattca caaaacttga aacaccacta tcagctcttg ctttttcact
       61 tccagaataa ggaaccagag aaacttttcg gacttattga ggacaatctg aagcaggttc
      121 atcctatttt tcagactgtc tttaaaacct tcttcaaaga taaggaaaag attatcaacg
      181 cccttcaact acactattct aatgccaaac tggaagcgac caataatctc atcaaactta
      241 tcaagcgcaa tgcctttggt tttcgaaact ttgaaaattt caaaaaacgg atttttatcg
      301 ctttgaacat caaaaaagaa aggacgaaat ttgtcctttc tcgagcttag cttttttcaa
      361 cccactacag ttgacaaaga gccgaaaaat cccttgttta gtaaacaaag gattttgtga
      421 attttcaatt tgattaaagt tcgatatcac caaacaaatc agccattgaa aatcctgttt
      481 gtgtttctgg aagttcgaaa tcacgctttt cttgacgtct tggacgacgt ggacgagcag
      541 cacgtttttc ttctttttgt ccttcttctt gggctggacg ttcttcaaga gctttaatag
      601 aaagtgatac gcgttctgca tctgcgttaa cttcaagaac tttaacttga acttcttgac
      661 caactttaag agcttctttt ggattttcaa tccgtttgtg tgaaatttgt gatacgtgaa
      721 caagtccatc gatacctggc aatacttcaa caaatgcacc gaagtcagtc aaacgtttaa
      781 ctgttccttc tactacatca cctttagcca atttttgctc aacgccatcc catggtcctg
      841 gtactgttgc tttaagtgaa agtgatacac gtccttcttc ttcgttaaga tcaaggattt
      901 tcacttcaat ttcttcacca acagttacaa ctgattttgg tgatacgtta cgttcatgtg
      961 acaattcagt caagtgaacc aatccgtcaa caccaccaag gtcgatgaaa gcgccgaagc
     1021 ttgtgatacg agcaacttta ccagttacaa catcaccaac agccaattta ccgaatactt
     1081 cagcgcgagc tgctgcagta gctgcttcaa caacttcacg acgtgaaagg atgaagcggt
     1141 tttctttagc gttaacttct ttgattttag tatcaaattc ttgacctaca aaacgctcag
     1201 cgttacgtac gaaacgagta tccaacattg aagctgggat aaatccacga acaccttcaa
     1261 attctactga aagtccacct ttaacggcac gcgttccttt aacagtaaca acttcttctt
     1321 cgcgaccaac aagtttgtcc catgctttgc gagcttcaag gcgtttttta gatacaaggt
     1381 atgtaactgt atcagtatct ttaccaacta cttgacgaag tacaagaaca tccaatactt
     1441 ctcctacttt aacaaagtca ttgatatctg catcacgatc gtttgtcaat tcgcgaagag
     1501 tcaagacacc ttcaacacca gttccagaga ttgcaacgtt agcttgagtc gcatcaactg
     1561 tcaatacttc agcactaaca acatcaccag tctcaacttg actaacgcta tttagcaaat
     1621 cttcaaattc gttcatctaa aaaatcctcc aacaatcaag tttttcttaa acttgacata
     1681 cttataattt ttttcctaag cacccgcaag gacatgttca tatcattcac gtctttcaat
     1741 tataaaaata aaatacccta agatattttg ctttttatct tgattattac ctaagaactg
     1801 gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc
     1861 tataccccat taatgaaatg gagagagagg gattcgaacc cccgaacccg aaggagcgga
     1921 tttacagtcc gccgcgttta gcctcttcgc tatctctcct acaatcaaca tggactatta
     1981 tatcatgaaa atttcaaaaa aacaagactt attttgctaa attataattt tcaatcaaaa
     2041 tttccacagc tttttccaat tttttataaa aactatcgac attcccattc tttataatgg
     2101 caaattgtcc gtctaattcg gcaatttctc cgataacttt tttaccgata gtcaatgtat
     2161 aaccttcaaa actgtcttta ccaacattaa ctttggcatc cgctacttga atttcgattt
     2221 ttttatcttt cttgctcatt tcatacctct cttcactttt tctattttac aagaaaatcc
     2281 taaaactagc aagaaaaaac tctatatcag gcttctctga tatagagttt tatgtaaaca
     2341 acctcaatcg atttagcata gctaatagtt agtaaacaag cataattgac tatcctttct
     2401 aggttatcag tgagtctcac cgcctactaa ctgcaaatcc ttatctttgt agtctacaat
     2461 agcttcaata gctacctcta tgcctcttac aatatcacat agactcattc ctgcagtatt
     2521 cggcttattt actacttgtt ccatcatata tggaatatgc atgaaacctg ctctcatatt
     2581 agggaatttt ttatccacta aataaagagc ttggtacatc aaatgattgc aaacaaaggt
     2641 tcctgcacta ttggatacaa ctgccggaag tccctgtttt ttgatagctt gtaccatcgc
     2701 tttgataggt aaactactaa aataggccga tgctccatca atacgaatcg gtgtatcaat
     2761 tggttgattg ccttcgttat caggtatgcg agcatcatct tgattaatag ccactcgttc
     2821 aggtgttaag ccggtcctgc cgcctgcttg tccaatacaa agtacagcat ctggttgata
     2881 tcgtaatatt tctgcctcta aaacttctga cgacttataa aaaaccgttg gaatttctac
     2941 ccagcgaact tcagccccat taatctcaga tggtaataat tttacagcct ccaaagctgg
     3001 attaatcttt tcacctccaa aaggattaaa acctgtaacc aatattttca ttttattttc
     3061 ctttactaaa atgcgagaaa gtacattaag aatatgtgaa taacaatcat tactagagca
     3121 acacctgctt gagcctttat aacgccattc tgatctttca tatccatcaa tgctgctggt
     3181 agagcgttaa aattagcagc cattggggtc aataaggtcc cacaataacc tgctgtcatg
     3241 gcaagagcac cagccacaat tggattagct cccagagcaa atacaaaggg aactccaaca
     3301 cctgctgtaa taacggtgaa tgctgcaaaa gcatttccca taatcattgt gaatagaacc
     3361 attccaagaa cataggccaa aactcctata aagcgactat ctgaaggaac aataccgcta
     3421 atcagatgag agataacatc accaacacct gctacagtaa aaatagcccc caaagcccct
     3481 aataattgag gaacaatacc acttgttgaa acttgctgag tcattcgatt attttctgat
     3541 aacagactct tagggtgact attggtaatc acaagaacag aaattgtagc aaacaaggcg
     3601 gcaaggctaa tcgaaatctt gctaaattct ggaatcattt gcgctaagac caacgcaagt
     3661 attgccatca gcataactgg aataaaaatt ttatttttca acctgttaga ttcaatattg
     3721 gctttcattt catctaagga tggcaaggtt ccgatacgga cttgcttaaa caatgttaac
     3781 agcgataata ggattacaat aataccaata ctcatatttg gcatatagga accacctata
     3841 aacgtaatag acaatagagt ccaaaatgca gatgtcccaa gtcgaactgg gtttgtttta
     3901 tctttataac tacaataggc tgtatggaga aattgacaac caatcacaat ataggtcaac
     3961 tctaatagtt gctttgccaa ctctgtcatt tttgttctcc tcccctagtc ttttttgata
     4021 tcaatttttt atcaaataaa taattgtaaa tccccactac aataagtgtt ataacagcaa
     4081 caataataga tgtagaagca atccctgcat aattgctttc atagcctaac tgatctaatg
     4141 ttccccctat caagaggact cccccagcac ctacaaacgt attttgagca aagaaatttc
     4201 caaaattttc attcgcagcc gcacgcgctt ttattgtctc atcttcaacc tctgttaact
     4261 ttctacctaa ttgagactct gcagctgctt ctcccatagg ttgaaccaaa ggtctgacaa
     4321 actgagggtg tcctcctaga cgaattgaaa agaaaccagc taactctcga ataaagaaat
     4381 aaactgtata gaagtttcca actgtcagac ctttaatctt tcgaatcaaa tcgattgatc
     4441 gttgcttgag tccaaaggtt tctgacagcc ccacaagagg caaggtaacc ataaaaatcg
     4501 tgagcactcg ctgattgcta aattcttttc ccaaaatctc caaaaattca acgagagaaa
     4561 cacctgaaac taaagctgta accaaaccag ctaagactac tgttgcaatt gtatcaaatt
     4621 ttaaaataaa acccacaaca atgattgcta ttcctattaa tctaatccac tccatatcaa
     4681 actcctttat attcaaaatg acagtatttt taaaatttta tcaagatcaa taccattcct
     4741 tatttaatgt gtttttctag ttctttttgg tatttgctat tggattccaa tttttctttt
     4801 tgccattttt taaaaacctc gttatattct tttgttgtaa gaatatcttt ttgcaatttc
     4861 attcctttaa agatatatgg atccccctta ataccaactt gtgagtatgg ttttgagaat
     4921 ggtactacgt tacttacaac tggagaacca ccagatgaag ctgttggcat caataatgaa
     4981 ctatctgtcg accaagcttg agctttggca tatttttcat atcttttctc taggtcagtg
     5041 gtctcagaaa cagcatcttc taacaatttc ttatatttat ccaaaccagg tttagctaca
     5101 acatccttat cttttccttt cgtaatacca aggtgtttca tggcagaacc agattttgga
     5161 tctataatat tcaagtaaga cgctggatct tgatagcttg gagcccatcc tgtactattc
     5221 aaatcatagt ctttttgaga aggagcaaca ttgccgcatt tatcattttc catcaaacca
     5281 tcaataacat ttccaataac gtctgtcgtc gatgttcgag tcgctatact gtagcccaat
     5341 gatgctggat ctactgcata gacataagaa aatgttgtcg gtgcatctgc ttctttatca
     5401 gtttttccac aagccactaa aatagctgac gtgctcagga ccactcctgc tgttaagagc
     5461 cactttttct atttcataaa aaatctcctt tggtttattt taatctactt ttacaatcca
     5521 accttctggc gcttcaatat cgccaaactg aatacccgtc aattcattat ataatttacg
     5581 cgtcacagga cctacttctg tttcactata gaatacatgg aaatcatcac catgttgaat
     5641 acctccaatt ggagaaataa ccgctgctgt accacaggca cctgcctcta caaaacggtc
     5701 aagattatca attggaacat caccctcaat aggagttaat cccaagcgat gttctgccaa
     5761 ataaagcaag gaatacttgg taatagatgg caagatagat ggactcaatg gtgttacaaa
     5821 ttcattatca gctgtaattc caaagaagtt agctgatccg acttcttcaa tctttgtatg
     5881 agttgatggg tccagataga taacatctga gaaatgacgt gacttggcca tttttcctgg
     5941 taagagactt gcagcatagt ttccaccaac cttagccgca cctgtaccat ttggtgctgc
     6001 acggtcgtac tcatcctgaa tcaagaagtt ggttgggacc aaaccacctt taaagtaatt
     6061 tccaactggc atagcaaaga tggtgaaaat gtattcttct gccggtttta ccccgataat
     6121 atctccgaca ccaatcaaaa gagggcgaag atataaggtt ccacctattc cgtatggtgg
     6181 tacgtattct tcattcgcac ggacaactgc tttacaagct tctacaaaca tgtctgtcgg
     6241 aacttgtggc atcaagagac ggtcacatgt acgttgcagg cgtttagcat tttcatcagg
     6301 acggaacagt tgaacactgc catccttagt acgataagct ttcaaacctt caaatgcttg
     6361 ttgtccatag tgaagacttg gagaagactc tgaaatatgc aaagttgcat cctctgtaag
     6421 ctctccttga tcccattgtc catttttgaa atgagcaaga tagcgataag gtaatttcat
     6481 ataggaaaaa ccgaggtttt cccaatcaat cgttactgtc atattccact ccttattcta
     6541 aaaacctatt tcttatattc tacactattt ttctaaaata gcaagtatat tttgtaattt
     6601 tcagaaaatt tctccaataa aaaccagctc ttagaactga ttcttcattt cacttattta
     6661 tcttcagtaa ctacttcctg aagataagcg tcaaaaactt cttcatctga aatcgtgtca
     6721 gaaatgaagc ttccattgct agtgcgttct gacaagttca agtcttgcaa tcggctttca
     6781 tagattgttc ctttattgga ttggacaagc agagtttggt cgttcacatc cacttccgta
     6841 ctgaagaaat cgccaacaaa tccttgctct gcaactgctc ctgccaagaa gacacgatgc
     6901 ggtttgtttt tcaactcacg caagacttgt aatcctcgtt tggcacggct ggttgctaga
     6961 atttcctcaa tggaaacacg tttcaagctt ccacgctggg tcaagaggta gaaggacgaa
     7021 gtattacaga taaagccaga ttggaggaca tcatcttctt tcaaattcat agccttgaca
     7081 cctgctgcct tagcaccgac aaccggaacc tcttcgatat tgaaacgcag ggcataacca
     7141 ttttgactaa ccaagacaac atcatctagt ttaatcggag ccactgctac aatctgatct
     7201 gtatcgtctt tgagcttagc atacttgaca gacttagatc tataggtccg ccatggagta
     7261 aattcttttc gctctacccg tttgatttga ccaaggcgag tcgctgcaaa gtaggttgtc
     7321 gcatcgtcaa actgatccag tacttccaca taaagaattt cttcattcgt ttcaaagttt
     7381 gtgatggttt ggctcagatg ctctccgatg tccttccaac gaatatctgc caactcatgg
     7441 attggtctgt agatgacatt tccaagactt gtgaacatca agaggtgctg ggttgtcttg
     7501 gcagattgaa caaaaatcaa acggtcatca tcacgcttgc caatttcttc caaggtggaa
     7561 gccgcaaagg aacgtggact ggtacgcttg atgtaacctg ccttggtcac gctgacgtag
     7621 gtatcttcct cagcgataag actagctgta tcaatctcaa ttgctttcgc agtatcttct
     7681 aaagaactca aacgaggagt tgcaaatttc ttcttgacct cacgaagttc tttcttcatg
     7741 agattgtaca tagtcctttc atcaccgata atagccgcca gcatagcaat cttctcacga
     7801 agctctgctt cttcttcctg caagacaacc acatcggtat tggtcaaacg gtacagttgc
     7861 aaagttacga tagcctcagc ctgttcttcc gtaaaatcat agctaacttt gaggttttcc
     7921 ttggcgtccg ccttattctc agaagcacgg ataagagcaa tgacttcatc caaaatcgaa
     7981 atcacacgaa tcaaaccttc gacgatatgg agacgtttct cagccttttc tttgtcaaag
     8041 cgtgaacgcg ccaaaatcac ttctcgacgg tgagcgatgt agctagacag gattggaaca
     8101 atcccaacct gacgaggtgt gaaattgtca atcgccacca tattaaagtt gtagttgatt
     8161 tgtaggtcgg tgtacttaaa taagtagttg agaacaagct cagtattagc gtctttctta
     8221 agttcgatag cgatacgaag accatcacgg tcagactcat cacgaacctc agcaatccca
     8281 gctaccttgt tattaacacg aacatcatcg attttcttga ctagattggc cttattgatt
     8341 tcataaggaa tctcagtaat aacgatttgt tccttaccac cttttagctt ttcaatttca
     8401 gtcttggaac gaacaaccac gcgccctttc ccagtctcat aagctttctt gatttcatca
     8461 cgaccctgaa taatagcccc tgtagggaag tctggtccag gcaagaattc catgagttta
     8521 tcaatctttg cagttgggtg gtcaatcatg taaactgcag catctatgac ctcagctaaa
     8581 ttatggggag gaatgtctgt ggcataacca gccgaaatcc cagtcgaacc attgaccaag
     8641 aggtttggaa aggctgctgg caagaccgtt ggttctttct ccgtatcgtc aaagttccat
     8701 gcaaaaggaa ctgtattttt ctcgatatcc tgaagaaggt agcctgcaat ttcagacaaa
     8761 cgtgcctcag tataacgcat agccgcagga ggatctccgt ccatagaacc gttattaccg
     8821 tgcatttcaa ctagaatctc acgatttttc cagttctgtg acatacgaac catggcatta
     8881 tagatagaag aatccccgtg tgggtggaaa ttccccatga tgttcccgac tgacttggcc
     8941 gacttacggt agctcttgtc aaaagtattg ctatccttat tcatagaata aagaatacgg
     9001 cgctgaaccg gcttcaaccc atcacgaata tctggcaaag cccggtcttg aataatgtac
     9061 ttggagtagc gaccaaagcg ctctcccatg atgtcctcca gggacatgtt ttgaatgtta
     9121 gacataagat acaaagccca taaaatacca agtgaaaata gaaaattctt gaagtaagca
     9181 aactcacaag agaatttatc tttttcacac agtatctagg gcgtgttcaa ctcctttcaa
     9241 agaatgtaga gtaggttttt atgcagtaaa agatatttta cgggaattcg tcccgtgttc
     9301 agttacgata agtaaccaaa ctatcctgtt tgtatttttc aatatgaaaa tctggttttc
     9361 caaaattagt cttagtttgt gtcttagccg ctcccttaaa cgcctctttg agataagcac
     9421 tcatagaaga ttcttcatta ataatcctgc aattttttca aaccaagatt ttcaaactgc
     9481 ttttttacat agtcattcac atccgactct aatttccagt ttactaacat attattttct
     9541 ttcattaaaa cactgtcgct tcttctagcg taaacttgac attatcttca atccatttac
     9601 ggcgtggttc taccttatct cccatgagaa cattgacgcg gcgttcggcg cgcgctaaat
     9661 cttcaattgt gacacggatg agggtacgtg tttctgggtt catggttgtt tcccagagct
     9721 ggtccgcatt catctcacca agtcctttgt atcgttggag ggtagcgcct ttaccgaact
     9781 gtttacggag ttcttctagt tctccgtccg tccaagcgta ggccacttct tctttcttgc
     9841 ctttaccttt ggacatcttg taaagaggtg ggagggcaat atagacatga cctgcctcga
     9901 ctagcggacg catgtaacgg tagaaaaatg tcaagagcaa ggtctggata tgggcaccgt
     9961 cggtatccgc atcggtcatg ataatgatct tatcatagtt ggcatcttca atagagaagt
    10021 ctgctccaac acccgcacca atggtataaa tcatggtatt gatctcttca tttttgagga
    10081 tatccgccat cttggccttg gctgtattga caaccttacc acgaagaggt agaatagcct
    10141 ggaacttgcg gtcacgacct tgtttggcag aaccaccggc agagtccccc tcaactagat
    10201 agagttcatt cttagcagga ttcttagatt gggctggggt caatttccca gacaacaagc
    10261 ccttatcttt cttgtttttc ttcccatttc ggctctcatc acgcgcctta cgtgctgctt
    10321 cacgagcatc acgggccttg atagccttgc ggatgaggtt agaagctaat tccccatttt
    10381 ccataaggaa aaaggtcaac ttatcagcca ctattccatc cacaactggg cgagctaggg
    10441 ggcttcctag tttatccttg gtctgtcctt caaactgcaa gtgttcttca ggaactaaga
    10501 tagaaagaac ggccgctagt ccctcacgat agtctgaacc ttcaaggttt ttatcttttt
    10561 ccttgagaag acctgtttta cgtgcatagt cattcatgac cttggtaatg gcagacttga
    10621 gtcctgtctc gtgcgttcca ccgtccttgg tgcgaacgtt attgacaaag gatagaatgt
    10681 tatctgagaa tccgtcattg tactggaggg ctacttccac ttgaaaacca ttgtcttccc
    10741 cttcaaagta aagaactggc gtcaagattt ccttatcttc gttgagataa gaaacaaaat
    10801 cttgtactcc attctcatag tggaactcaa tcgcttcatc tgttcgcttg tccgttaaag
    10861 acaaggtcac atttttcaag agaaaggctg attcattaag gcgctctgaa atggtattgt
    10921 acttgaaatc tgtcgtagaa aagatagtcg cgtcaggcat aaaagtaact ttggtgcctg
    10981 ttttagactt gggtgctgta ccgattttct tcaaagtcgt gacaggtttt ccaccatttt
    11041 cgaaacgttg cttgtaaact gcgccatcac gggtaatttc aacttctaac cagctagaaa
    11101 gggcgttaac aacggaagaa cccactccgt gaagtccacc tgatgtctta tagccacctt
    11161 gaccgaattt ccctccggca tgaagaatgg taaagataac ctcaacagtt ggaattccca
    11221 tagcgtgcat acctgtcggc atcccacgtc catggtcttg aaccgttaga ctaccgtctt
    11281 tattgatagt tacatcgata cgatcaccaa acccagacaa ggcttcatcg actgcattat
    11341 caacgatttc ccaaactagg tgatgaagac cagcgccatc ggtcgatcca atatacatcc
    11401 ccggacgttt tcggaccgca tccaaccctt ctagcacctg aatagcatca tcattataat
    11461 tgttaatatt gatttccttt tttgacacaa ggaacctcct attcgttcat ctttactatt
    11521 ctacaggttt tccaaggatt ttgcaaaatt tttctttctc cgatgtgaca atttcagcag
    11581 agattctctg cttttctttc ccaattcatg atataatagg agtatgatta caatagtttt
    11641 attaatccta gcctatctgc tgggttcgat tccatctggt ctctggattg gacaagtatt
    11701 ctttcaaatc aatctacgcg agcatggttc tggtaacact ggaacgacca ataccttccg
    11761 cattttaggt aagaaagctg gtatggcaac ctttgtgatt gactttttca aaggaaccct
    11821 agcaacgctg cttccgatta tttttcatct acaaggcgtt tctcctctca tctttggact
    11881 tttggctgtt atcggccata ccttccctat ctttgcagga tttaaaggtg gtaaggctgt
    11941 cgcaaccagt gctggagtga ttttcggatt tgcgcctatc ttctgtctct accttgcgat
    12001 tatcttcttt ggagctctct atcttggcag tatgatttca ctgtctagtg tcacagcatc
    12061 gatcgcggct gttatcgggg ttctgctctt tccacttttt ggttttatcc tgagtaacta
    12121 tgactctctc ttcatcgcta ttatcttagc acttgctagt ttgattatca ttcgtcataa
    12181 ggacaatata gctcgtatca aaaataaaac tgaaaatttg gtcccttggg gattgaacct
    12241 aactcatcaa gatcctaaaa aa
//
LOCUS       DAVKGU010000056        11968 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKGU010000056 DAVKGU010000000
VERSION     DAVKGU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.056"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(50..1099)
                     /locus_tag="VW077_001437"
     CDS             complement(50..1099)
                     /locus_tag="VW077_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9455437.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKIGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(1219..3027)
                     /gene="glmS"
                     /locus_tag="VW077_001438"
     CDS             complement(1219..3027)
                     /gene="glmS"
                     /locus_tag="VW077_001438"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9455438.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(3219..4598)
                     /gene="bglA"
                     /locus_tag="VW077_001439"
     CDS             complement(3219..4598)
                     /gene="bglA"
                     /locus_tag="VW077_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9455439.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYDNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(4697..6550)
                     /locus_tag="VW077_001440"
     CDS             complement(4697..6550)
                     /locus_tag="VW077_001440"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9455440.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     SDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(6563..7822)
                     /gene="rseP"
                     /locus_tag="VW077_001441"
     CDS             complement(6563..7822)
                     /gene="rseP"
                     /locus_tag="VW077_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9455441.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(7844..8647)
                     /locus_tag="VW077_001442"
     CDS             complement(7844..8647)
                     /locus_tag="VW077_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9455442.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(8656..9414)
                     /locus_tag="VW077_001443"
     CDS             complement(8656..9414)
                     /locus_tag="VW077_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9455443.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(9645..10109)
                     /locus_tag="VW077_001444"
     CDS             complement(9645..10109)
                     /locus_tag="VW077_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9455444.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTAFYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(10198..11169)
                     /gene="ruvB"
                     /locus_tag="VW077_001445"
     CDS             complement(10198..11169)
                     /gene="ruvB"
                     /locus_tag="VW077_001445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9455445.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            11330..11422
                     /locus_tag="VW077_001446"
     CDS             11330..11422
                     /locus_tag="VW077_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9455446.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         3508 a         2818 c         2251 g         3391 t
ORIGIN      
        1 attttcgtac aatatttgtt gtcctaataa acttgattag gattttttat taagcctctt
       61 tcatagcaaa ataagctcgt actttgggtg caacttgtgt tccgaagagt tcaatagctc
      121 tcagaacctg gtcatgaggc atagaaccaa gcggtagatg aagcatgaag cggtccaatc
      181 ctaaatcctc tatcatgcga atcaattttt cggccacctg atctggattg ccaacaaaca
      241 tggcgccatt tggccctacc tgctccaaat attgctcata acgcaattcc tgccagtgcg
      301 gacggtcttt ggaaatagca tccactactt gcttagtcgg atggaaataa tctttcaccg
      361 cctgctcacc atcttccgca atccaccccc aagaatgggc tcccactttc aagtctttgt
      421 cagcatggcc cgcttcgctt ccaatctcac gataagcctg aatcaacttt ttaaaataac
      481 gtggattacc accaataata gcatatacaa tcggtagacc agcctgagca atcttcactg
      541 ttgattcgac atgaccacct gtagctatcc acaagggcaa tttgtcctga actggacgag
      601 gataaacttc tttaccagca atcgtttgag tcaatcgacc ttgccagtct aacttggtct
      661 tttcattgac taactgaagc aagtctaatt tctcatcaaa aagagagtcg tagtctttca
      721 agtcataacc aaacagaggg aaagattccg tgaaagagcc ccttccagcc ataatctccg
      781 atcgtccatt tgacaaagca tcgatagtgg catactgttg gaacaaacga atcggatcca
      841 tgcttgacag aatgctgact gcactggtca aacggatttt cttggtattg actgccccag
      901 cggccaggac aatctctggg gctgatactg caaaatccgc ccgatggtgc tcgccaatcc
      961 catatacgtc caaaccaatc ttgtcagcca gctcaatctc tgccaccaac tggcgaatgc
     1021 gttcagcatg actgtaagtt tgtccagtcc cttcaagctc cgttatttcc ccaaatgttg
     1081 aaattcccaa ttctaccatt gtgattctcc ttgtctatct ctgtccttca atttgaaaaa
     1141 ttattctaac acgaatctta agtacaagca accaatttgc tcacaagaaa aaggcctaga
     1201 taactagact tttttagctt attctaccgt tactgacttg gcaaggttac gtggtttgtc
     1261 cacatcgagg ccacggtgga gggttgcaaa gtaagcgact aattgcgttg gtacgaccat
     1321 tgaaattggt gagaggtatg gatgtacggt cgtaaggacg atatcgtcgg tatctttggc
     1381 tacattctct tctgcgatag tgaggacttt ggcaccacgg gctgcgacct cttggatatt
     1441 tccacgagta tggttggcaa gaactggatc tgacaagaga gacaagacag gcgttccttc
     1501 ttcaatcaag gcaatggttc cgtgcttgag ttctcctgcc gcaaaacctt cacactggat
     1561 ataagaaatc tctttgagtt tgagacttgc ttccatggct acgtagtaat cttgaccacg
     1621 tccgatgtaa aaggcgttac gagttgtttc aagaagttca cgaaccttgg cttcaatggt
     1681 ttctttctct gaaagagttg attcgataga ctgagctacg attgacaatt catgaaccag
     1741 gtcaaaggct tgcgctttag cattaccatt tgcttctccg actgcttttg caaggaaggc
     1801 aagggctgcg atttgcgctg tataggcttt agttgatgcc acggcaattt caggacctgc
     1861 gtgaaggagc atggtatagt tggcttcacg tgagagggtt gaacctggaa cgtttgtcac
     1921 tgttaagctt ggaattccca tttcattagc cttgaccaaa acttgacgac tatccgctgt
     1981 ttcaccagat tggctgataa agatgaagag tggtttcttg ctgagaagtg gcataccgta
     2041 gccccactca gatgagattc caagttcaac tggtgtatct gtcaattctt ccaacatttt
     2101 cttagaagca aatcctgcat ggtaagatgt tccagctgca aggatgtaga tgcggtctgc
     2161 gtcttgaaca gccttaatga tagcaggatc aatcactact tgaccagcat catccgtgta
     2221 ggcttgaatg agtttacgca taacagttgg ttgctcatca atttccttaa gcatgtagta
     2281 aggataagtt cccttaccga tatctgacaa gtcaagttcc gcagtatagc tagcacgttc
     2341 acgactgtta ccatcatagt cttgaacttc cacgctatca gccttgacga ttaccaactc
     2401 ttggtcatga atttccatgt attggttggt ttcacgaatc atagccatag catctgagca
     2461 gaccatattg tagccttccc caagaccaat caaaagtgga gatttgtttt tcgctacata
     2521 gatgacatct ggattttcag agtcaatcaa ggcaaaggca tatgaaccac ggataatatg
     2581 aagagctttt ttaaaggctt caagaactga gagaccgtct tcttccgcaa attttccaat
     2641 caaatgtacg gcgatttccg tatctgtttg ccctttgaag tggtgcccag caaggtattc
     2701 ttctttaatt tcaaggtagt tttcaatcac cccattgtga accaagacaa aacgttctgt
     2761 ctcagagcgg tgtgggtgag cattgtcttc cgttggtttc ccatgagttg cccaacgagt
     2821 atgtccgata ccagttgttc cctcaacacc agctgtcttg gcagacaatt ctgcgatacg
     2881 accaacagcc ttcaccaaat ggttatcagc accatctagg acaaaaattc ccgcagaatc
     2941 atagccacgg tattcaagct tttcaagccc ttgaatcaaa atatcagttg catttgtgtt
     3001 tccaacaaca ccaacaattc cacacatagt atatacgaca caggcaagct gtgctttctc
     3061 cttaaaattg gtatagtcta attcatcttt tatagaatca gcaaaaacag tatatacttg
     3121 tttctttcac ttgtcaagag taaaaattgg tatagttcaa attaagctcc tgtaagcaaa
     3181 aaactctgac cgattgggat aatcagtcag agtccttttt aaaatccatt attatcgctt
     3241 aattctttga accagtggcc tgatttcttc agacgacgtt cttgcgtttc caagtctaat
     3301 tcgaccaaac catagcgatt tttatagctg ttgagccatg accagcagtc aataaaggtc
     3361 caaatcaagt agcccttaca gttggcacca tcttcaatgg cacggtgaag ttcacgaaga
     3421 tgacctttta caaagtcaat acggtaatca tcttgaatca ttccatcttg acggaatttt
     3481 tcttcccctt caacacccat accattctca gtcaacatcc attcaatatt gtcataattt
     3541 tccttgatat tttgggcgat gtcataaatc ccttgctcat aaatctccca accacggtga
     3601 gaattgattt tacgtccagg catcacataa ggctcgtaaa aatgttctgg taagagtgga
     3661 ctatctggat gcttagcaaa tcgaggagcc ataacacgca aaggttgata gtagttcaca
     3721 ccaaggaagt ccaccgtatt atcacgaatg agttccaact cttcctctgt agcatcaggt
     3781 aaaagaccgt gttcatgcaa gatttctacc aactcctgtg gataagtccc caagacagat
     3841 ggatctaaga aagattgggc ctgaaaaagg gccgcaatac gagctgcctt gacatcagca
     3901 ggatgctggc tacgtggata agccggtgtc aagttgagga caatcccaat cttggaatca
     3961 ggcaaaagtt catggcaagc cttaacagcc cggctgctgg ccaattgtgt atgataggct
     4021 accttaacag ctgcctctgc atccacctta tgtggataat gggcatcata aaaataacca
     4081 aattctacag gaacgatggg ctcgttaaag gtaatccatt gatccactaa atctccataa
     4141 gtctcaaaac aaaaacgagc atagtcttca taggctgaga ctgtcgcctt attttcccaa
     4201 ccatcaccat cctcttgaag ggcaaaaggt aaatcaaaat gatagagatt gactaacaga
     4261 cgaattcctt tagccttaat agcctcaaag accttacgat aaaaatccac accttgaggg
     4321 ttgacttttc cacagccttg tggaaaaatc cgtgaccact gaatagaagt ccgaaaggct
     4381 gtgtgaccag tctctaacaa aagctcaata tcccgctccc aattttcata aaaagtcgat
     4441 gtcttatctg aaccaatccc attatagtaa cgatttggct ccacttggaa ccagtaatcc
     4501 cagagattgt ctcccttacc gtcaccagct acacgtcctt ctgtctgcgg tccagaagta
     4561 gaggatcccc agacaaaatc ctttggaaat cttagcatac atttacctct ttatctactc
     4621 atttctccca ttatacagaa aaaacaaggt aaaaactagt tacatttttt ccttgttttt
     4681 cttctgatta tagtttttat ttcttgctta ggatttcaag cgtttcaagc acgttatctg
     4741 catgaacctc aatggtgtca ccagttgcct tgatcttaac ttctacaatg ccatcggccg
     4801 cttttttacc aacagtgata cggattggaa gaccaatcaa gtcactatcg ctaaatttaa
     4861 caccgacacg ttcgttacgg tcatctgtca agacttcata accagctccc atcaagcttg
     4921 cttcaagttt ttctgtcaag gcttgcgctt cttcatcctt gacattaaca gtaatcaaat
     4981 gcacatcaaa cggtgccaat tctttaggga aattgattcc ccaagcgtaa cggtattcac
     5041 cttttggcgt tttgttaaca aagaggcgag cgtgttgctc catcactgct gaaagaagac
     5101 ggctgacacc gataccgtaa catcccatga tgattggcac agcacgacca ttttcatcca
     5161 agacatctgc tcccatgctt gctgaatagc gagttccgag tttgaaaata tgaccgatct
     5221 caataccacg cgcaaagtta aggacacctt gtccatctgg ggaaatttca ccctcacgaa
     5281 cttcacggat atccacatat tctgcagtaa aatcacggcc tgggttcaca ccagtcaagt
     5341 ggtagccatc ttcgttagca ccgacaactg cattgcgaac atcttgcacc ttacgatctg
     5401 caataatttt aatattctct ggcaaaccaa ctggtccaag tgaaccaaat cctgcttgaa
     5461 caacattcgc cacttcttct tcgctagcaa cgtcaaagaa atctgctccc aagtgatttt
     5521 tcaacttgac ttcgttgagt tggtcacttc caactagaag ggctgcaaca agctcgccat
     5581 ctgcaatgta gaagagggtt ttaatcgttt gttcttctgg aacattgagg aaggctgcaa
     5641 cttcatcaat tgatttaaca tctggcgttg caacacgagt aacttcttct tcagcgacaa
     5701 cacggttgct tggtttgtac tcgtttgttg ccatttctaa gttagctgca tagctagact
     5761 cacttgagta agcaatggta tcttcaccag agactatcca tttgagcaat tctgccttga
     5821 tttcttcttg cacttctgca ggaatttcgt caaatgaggc aactgacttg tccaagacaa
     5881 cccagcggtc aaggtctgta cgagcagatg taatggccat aaattcttgg ctatccttac
     5941 cacccatggc tccaccgtca ccaataatag ccttgaagtc taaaccacta cgagtgaaaa
     6001 tacgctcata ggctgctttg tactcatcat aaacactatc caaactatca tagttagcgt
     6061 ggaaactata agcatccttc atgataaact cacgtgtacg aagaagtcca ttacgcgggc
     6121 gtttttcatc acgatacttg ggctgaattt gataaaggtt gagtggcaat tgcttgtaag
     6181 atttaacaga atcacggaca atagctgtaa aggtttcttc gtgagttgga cctaagataa
     6241 agtctgattt ttcacggttt tttagtttgt aaaggtcttc accataggtt tcgtaacgac
     6301 ctgattcacg ccacaattct gcactaagaa gggcaggagc caacatctca acagcaccaa
     6361 tcttttcgaa ttcttggcgc atgatgtttt tagctttttc aatcacacgg ttggcaagtg
     6421 gtagataaga ataaacacct gctgaaactt ggcgaacata accagcacgc aacataagag
     6481 catggctgat aacttgagca tcgcttggca tttcgcgaag cgttgggata ggcattttac
     6541 tttgtttcat aatattcctc gattatctaa aaaagagtcg cataatgtca ttccaagtca
     6601 cagcaatcat caagacaacc atgatgacca ctccggccaa ggtgacatag gtttcaattt
     6661 cttgtttcaa tggtttgcgg cggatggctt ctaggatatt gagcacaatc ttaccaccat
     6721 ccaaggctgg aatcggaata agattaaaaa tcccaatatt gatggaaatc attgccaaga
     6781 agtacaagat attttcaatt ccatttttag cagcatcact acttgcctta aagatagcaa
     6841 caggtccacc caacttgttc aaatccggtt ggaaaatcag atttttcaga gctgagagaa
     6901 ttcggagagc tgagtcagca gcagttgtaa aaccacctac aaacatggat agaaaatctg
     6961 acttaacccc cggttgaaca cctagaaggt aacgaccttg actatcttcg ggtgtaacag
     7021 tgacttgttt gtcactcccc ttttcagaaa tagtcacatc caaagtcggt gccgtcttat
     7081 ctttggtttc tgtttccaca gcttggatca agctttccca gttgctaacc tcatgtgagc
     7141 caatcttggt aatttgtgcc gtttctggta ctcctacctt ggccaaggca ccttggggca
     7201 tgatatggaa ctgattggta tcaacatctc tgacaccacc ctgcataaag attaaaaccc
     7261 aaaaaacaac gacacctaag ataaaattgt tcataggacc tgcaaaattg gtaatcagtt
     7321 tgccccagat agtcgcattt tgatattgaa catctaaagg tgcaatccga acctcagtac
     7381 catctgcttc cacaaccgtt gcatcgtgat ccactgcaaa tgttttttct tcttccagaa
     7441 ccaatccttt gataaagagc ttgtcttcaa aatcaaactg ggtcacctgc atagggaggg
     7501 ctgtttgatc caatttttta cctgagagat tgatgcgttt aaccttacca tcatcagcaa
     7561 gtgtcaaact aacaggcgtt cctgtcttga tttcagttgt atcatcaccc caaccggcca
     7621 tgcggacata gccacccaga ggcaagattc gaatggtata ggccgttcca tccttgccaa
     7681 tgtgagcaaa aattttaggt cccataccga tggcaaattc acgtactaaa atccctgatt
     7741 tcttggcaaa gtagaagtgc ccgaactcgt gcaccactac aataatcccg aaaaccagaa
     7801 taaaggttaa aattccgagc atagcgtttc ctccgtcttt tgattaaaag agtccaaata
     7861 agtgcatgat tggaaataca agcaacatac tatcgaaacg atccaaaaca ccaccatgtc
     7921 cagggataaa tttcccagaa tccttaacac caaaatgacg tttgatcgaa ctttctagta
     7981 aatcaccaaa ttgtccagca atgctaaaga aaatagcaaa gactgacatc ttgtaaattc
     8041 catatggaag agcaactgta ctgtcaacta tcataaagat aatggttact aaaattgctc
     8101 ctaaaatacc acccaaggca ccctcaaggg ttttattagg cgataccctt ggtgctaact
     8161 ttcgtttccc atagttcatc ccaacaagat aggcaccact gtctgtcgcc cagacgatac
     8221 acaaggctaa gagagccttg tccaaacctg caacacgagc atctagtaaa gcattaaatc
     8281 caaagcccac gtagaagctc atagcaagag ggaaaaccgc atcctcaatc gtataagact
     8341 tgctaaaaac ggtcgttcct aacatgattg aaatcaaaac actataggca accacattcc
     8401 catcaactgg caaaaaagtc aggtaattct ccaagggaat ggtcaatgca aaggttgcaa
     8461 agagggtcaa gaggccctcc atcgtcatgg tctctagacc tctcatcttc aaaagttcat
     8521 gcatggctag catggctatg attccgattg ctatctgaag caagaggccc ccaatcatta
     8581 aaattggtag gaaaatagcc agggcaatcc ctgcaaacaa ggttcttttc tgtaaatcct
     8641 gggtcatatt tcctcctaaa ctcctccaaa tcggcgatga cgacgattat aggcaagaat
     8701 agcttcctgc aaggccgctt cgtcaaaatc aggccataag gtgtccgtaa aataaagctc
     8761 actataggct ccctgccatg gaaggaaatt gctcaaacgt aattctccac tagtacggat
     8821 aatcaagtct gggtctcgta agtccttagg caaatgctgg gtaaagagat agttaccaat
     8881 caattcctct gtgatgtcac ctggattgat tttggcatct aaaacatcct gggaaatcaa
     8941 cttaagcgcc tgtgtaatct cagcacgtcc accatagtta agagcaaaat taagaatcaa
     9001 tcctgtgttg ttcttagtca attcctcagc cttggttaaa gcttcaaagg tttgcttagg
     9061 caggcggtct gtctccccaa tcatttgaat cttaacatta ttcgcatgta gttccgggac
     9121 ataattatca taaaactcta ctggcaagtt catgataaac ttgacttcct gatctggacg
     9181 ggtccagttt tccgtagaaa aagcatagac cgtaataacc ttgacgccca gtttgttggc
     9241 tgccttggtc acggtttgca atgcttccat gcccgcctta tgtccaaaaa ctcgcggttg
     9301 catacgtttt ttagcccaac ggccattgcc atccatgatg atgccgatat gagcaggaac
     9361 ctgtgtcgga acctctactt ccacagcctt atctttctta aaaaatccaa acatgatctt
     9421 attcctattc aaaaatctat cgtttcatta taccatattt ccccattttc ttctatcact
     9481 aagctattta ttctcaggca ccatgcccat ttttcaaaaa aataagccgc ctgattgggc
     9541 gactttattt ttatagggag attattatga aaaagtttta ggagtttaag ttaaggtctt
     9601 cttaacttat gaacttagtg tacactccct agcttaaagt ttccttaagt atttttaaaa
     9661 atcaaatttt tccatttctc ctgccaattt ttcttggata aacgtgtttg atagagttcc
     9721 attcggtctt cattttctaa gaaatgagga gttggacgaa cttgaaaatt caaaatatcc
     9781 tccaaaccat aaggtgcata aagttcaaaa tctgattctt cattcaagcg cagtccaact
     9841 gccgtacacc gttctggata cttactcata gcatcacgag aactggtata gaaagcagtg
     9901 tgaggactgt gctggtgcat atagacctga tttttcaatt cccactggta ctgaggaaaa
     9961 tcctctctca gctttttctc cagtaataag gtttcctcat aagaaaaatc tggatcaaag
    10021 aaaatcacat ctatatctgt ttcatgatca aaaggggatt tgtctgacaa aagattccag
    10081 atgaaatttc tgacagaacc tgctgccaac tacgagtctt tcaaaccaag gtctcggatg
    10141 atcgtcagaa tggccatcat atctggattt tctctaaaag cctctaagat ttcttgctta
    10201 tttttcactg tattcataac ctaagtgctc atatgcctta gcagtcgcca cccgtccaga
    10261 ccgtgtccgc atgataaaac ctttttgaat caagtaaggc tcatacatgt cttcaactgt
    10321 ctcacgctct tcggcgatat tgacagaaag agttcctaga ccaacaggtc ctccactgta
    10381 catctcaatc atggtacgaa ggattttttg atccacatag tccaaacctt catggtcaac
    10441 atccagcata gtcaaagcct tatcggtaat aatatcatcg attaccccat tccccattat
    10501 ctgggcaaaa tcgcgcacgc gcttgaggag acgattggca atacgagggg ttccacgact
    10561 acgtagggcc aactcagatg ctgcctcatg agtgatttcc atctcaaaaa tatctgccgt
    10621 ccgctcgaca atttctgtca agtcagcatg ggcataatac tccatatgac ctgtaatccc
    10681 aaaacgtgcc cgtagtggat ttgagagcat accagcccga gtcgtcgcac caatcaaggt
    10741 aaaaggaggc aactccaaat gaacactgcg actgccttca ccagccccaa tcataatatc
    10801 tatgtagaag tcctccatag cactataaag cacctcttcc actgacattg gcaaacgatg
    10861 gatctcatca ataaaaagga catccccagg ctctaactca ttcaaaatag ctaccagatc
    10921 tccggctttt tcaatgactg gacccgacgt ctgcttaaga ttgactccca gttcgttggc
    10981 aataacaaag gccatggtcg ttttcccgag acctggaggc ccaaataaga gcacatgatc
    11041 cagcgcttca tcccgcattt ttgcagcttc aataaagatt tgtagctggt ccttgacctt
    11101 atcctgtccg atatattcac gtaaatactg aggacggagc gtgcgttcta ctaactcctc
    11161 atcccccatt atctcattat ctaaaattct acccatggct ctattatatc aaaaaaccaa
    11221 gccacaaaca aaaaagccac ctgattgggt gactcctaag tttagcactt atgtggtata
    11281 atattatacg gcacttctac accgcctacg aaaggaggtg agatagccca tgatggaatt
    11341 agtactcaaa actatcatcg gaccaattgt ggtcggtgtc gttcttcgta tagtcgataa
    11401 atggctaaac aaggacaaat agtgtcaaaa aagacctcaa gcttatttgg tcgtgagctt
    11461 ggggtctttt ctagcctatg atatagaact agtactcaat tcctttttat tatcccatag
    11521 ttcacgaatt ttgtcaaagg tttacatttt cttcaaccgc tgtacgacaa gacggttaaa
    11581 attaagagaa cgttagggat tctatcaatt tcatagaaat tttgatttcg taaacgaaga
    11641 gacaatctta catgtcactt ttcatttaat acgccactac tagacaagca aaatcattat
    11701 tacagtagtt ccagtccttc aattaacagt cacttacaat caaattgagt ttgaactagc
    11761 tgaagcgacc acagacctat ttcttagtca tattcgctaa aaaaatcccc tccaaaatct
    11821 caaaaagtcc ccgccaattc cccgaccaaa atccgaaaaa taccgaaaaa tatcgaaaaa
    11881 ttatttttag aatagtccca aaaatcctga aatagagcta aaaaactcca cctgattggg
    11941 tggagttaag ggagattatt atgaaaaa
//
LOCUS       DAVKGU010000057        11788 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKGU010000057 DAVKGU010000000
VERSION     DAVKGU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.057"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            171..2462
                     /gene="pcrA"
                     /locus_tag="VW077_001447"
     CDS             171..2462
                     /gene="pcrA"
                     /locus_tag="VW077_001447"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9455447.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYCADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVAEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASIEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2505..3185
                     /gene="radC"
                     /locus_tag="VW077_001448"
     CDS             2505..3185
                     /gene="radC"
                     /locus_tag="VW077_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9455448.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3182..3871)
                     /locus_tag="VW077_001449"
     CDS             complement(3182..3871)
                     /locus_tag="VW077_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9455449.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3889..4530)
                     /locus_tag="VW077_001450"
     CDS             complement(3889..4530)
                     /locus_tag="VW077_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9455450.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4613..4828)
                     /locus_tag="VW077_001451"
     CDS             complement(4613..4828)
                     /locus_tag="VW077_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9455451.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4836..5183)
                     /locus_tag="VW077_001452"
     CDS             complement(4836..5183)
                     /locus_tag="VW077_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9455452.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5188..6303)
                     /locus_tag="VW077_001453"
     CDS             complement(5188..6303)
                     /locus_tag="VW077_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9455453.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6313..7272)
                     /locus_tag="VW077_001454"
     CDS             complement(6313..7272)
                     /locus_tag="VW077_001454"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9455454.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7385..7954)
                     /locus_tag="VW077_001455"
     CDS             complement(7385..7954)
                     /locus_tag="VW077_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9455455.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8082..8753
                     /locus_tag="VW077_001456"
     CDS             8082..8753
                     /locus_tag="VW077_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9455456.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8737..9555
                     /locus_tag="VW077_001457"
     CDS             8737..9555
                     /locus_tag="VW077_001457"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9455457.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9552..10448
                     /locus_tag="VW077_001458"
     CDS             9552..10448
                     /locus_tag="VW077_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9455458.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10492..11466
                     /gene="pta"
                     /locus_tag="VW077_001459"
     CDS             10492..11466
                     /gene="pta"
                     /locus_tag="VW077_001459"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9455459.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3431 a         2265 c         2534 g         3558 t
ORIGIN      
        1 gtattgagct agtttatgaa aaaattgggt gaaaaagtcg agtgttttag aaacccacag
       61 tgtagtattc tagtttcaat ccactatatt ttgctactcc ccgtaaagtt tctattttcc
      121 ctgatttctg atataataga aatattgact tcaagagtaa ggaagagaag atgaacgcat
      181 tattaaatgg aatgaatgac cgtcaggctg aggcggtgca aacgacagaa ggtcccttgc
      241 taatcatggc aggagctggt tctggaaaga ctcgtgtttt aacccaccgt atcgcttatt
      301 tgattgatga aaagctggtc aatccttgga atatcttggc cattaccttt accaacaagg
      361 ctgcgcgtga gatgaaagag cgtgcttata gcctcaatcc agctactcag gactgtctga
      421 ttgcgacctt ccactccatg tgtgtgcgta ttttgcgtcg cgatgcggac catattggct
      481 acaatcgtaa ttttacaatt gtggatcctg gtgaacagcg aacgctcatg aaacgtattc
      541 tcaaacagtt gaacttggat cctaaaaaat ggaatgaacg aactattttg gggaccattt
      601 ccaatgctaa gaatgatttg attgatgatg ttgcttatgc tgcccaagct ggcgatatgt
      661 atacgcaaat tgtggcccag tgttatacaa cctatcaaaa agaacttcgt cagtctgaat
      721 ccgttgactt tgatgatttg attatgctga ccttgcgtct ctttgatcaa aatcctgatg
      781 ttttgaccta ctaccagcaa aaattccaat acatccacgt tgatgagtac caagatacca
      841 accacgctca gtaccaattg gtcaaactct tggcttcccg ttttaaaaat atctgtgtgg
      901 ttggggatgc ggaccagtct atctacggtt ggcgtggtgc tgatatgcag aatatcttgg
      961 actttgaaaa ggattacccc aaagccaagg ttgttttgtt ggaagaaaat tatcgctcaa
     1021 ccaaaaccat tctccaagcg gccaacgagg ttattaaaaa caataaaaat cgccgtccta
     1081 aaaatctctg gactcaaaac gctgatgggg agcaaatcgt ttactattgt gccgatgatg
     1141 agctggatga ggctgtattt gtagccagaa ccatcgatga acttagtcgc agtcaaaact
     1201 tccttcataa ggattttgca gttctctatc ggactaatgc ccagtcccgt acaattgagg
     1261 aagccctgct caagtctaac attccttata ccatggttgg cggaaccaaa ttctacagcc
     1321 gtaaggaaat tcgcgatatt attgcttatc tcaaccttat tgctaatttg agtgacaata
     1381 ttagttttga gcgtattatc aacgagccta aacgtggaat tggtccaggt acagttgaga
     1441 aaatccgtga ttttgcaaat ttgcaaaata tgtctatgct ggatgcttct gctaatatta
     1501 tgttgtctgg tatcaagggt aaggcagccc aatctatctg ggattttgcc aatatgatgc
     1561 ttgatttgcg ggagcagcta gaccacttaa gcattacaga gttggttgag tccgtcctag
     1621 aaaaaacagg ttatgtcgat attcttaacg cccaagcgac tctagaaagc aaggctcggg
     1681 ttgaaaatat cgaagagttt ctttctgtta cgaagaactt tgatgacacc actgatgtgg
     1741 cagaagagga aactggtctg gacaaactga gtcgtttctt aaatgacttg gctttgattg
     1801 ccgacacaga ttcaggtagt caggagacat cagaagtgac cttgatgacc ctgcatgctg
     1861 ctaaaggtct cgaatttcca gttgtctttt tgattgggat ggaagaaaat gtctttccac
     1921 ttagtcgtgc gactgaagat ccagatgaat tagaagaaga gcgccgtcta gcctatgtag
     1981 gtatcacgcg tgcagagaaa attctctatc tgaccaatgc caactcacgc ttgctttttg
     2041 gtcgtaccaa ttataaccgt ccgactcgtt ttattaacga aatcagttca gacttgcttg
     2101 agtatcaagg tctggctcgt cctgcaaata caagctttaa ggcatcatat agcagtggta
     2161 gtatttcctt tggtcaaggt atgagtttgg ctcaggctct tcaagaccgt aaacgcggtg
     2221 ctgccccaaa atcaatccag tcaagcggtc ttccatttgg tcaatttaca gctggcgcaa
     2281 aatcagcatc tatcgaggca aattggtcca ttggtgacat tgctctccac aagaaatggg
     2341 gagagggaac cgttctggaa gtttcaggta gcggtgctag gcaggaattg aaaatcaatt
     2401 tcccagaagt aggtttgaaa aaacttttag ccagtgtggc tccaattgag aaaaaaatct
     2461 aattttccat ccttctcacg aataataaag tgaggaggat ttttatgtac agtatttcat
     2521 tccaagaaga ttcactatta ccaagagaaa ggctggccaa ggaaggagtt gaagcgctta
     2581 gtaaccaaga gttgctagct attttactca ggacaggaac acgtcaagct agcgtttttg
     2641 aaattgccca aaaagtcttg aacaatcttt caagcctaac ggatttgaaa aaaatgaccc
     2701 tgcaggaatt gcagagtttg tctggtattg ggcgtgttaa ggccatagaa ttacaagcta
     2761 tgattgaact ggggcatcgt attcacaaac acgagactct tgaaatggaa agtattctca
     2821 gcagtcaaaa gttggccaag aagatgcagc aggaattagg ggataaaaaa caagagcacc
     2881 tggtggcact ctatctcaat actcaaaatc aaatcatcca tcagcagacc atttttatcg
     2941 gttctgtaac tcgtagtatc gctgaaccgc gagagattct tcactatgca atcaagcaca
     3001 tggcgacttc tcttatcttg gtccacaatc atccttcagg agcggtagcg cctagccaaa
     3061 atgatgatca tgtcactaaa cttgttaaag aagcctgcga actgatgggg attgttctct
     3121 tggaccattt gattgtctct cattctaatt actttagtta tcgtgaaaag acagatttaa
     3181 tctaaagttc attaacgaca tagtcaaaga gtttcttatc tttgggacga ttttcaaaaa
     3241 gaagttctgg atgccattgg acaccgagaa aggcgacatc atccgtactc atgacagcct
     3301 caatgatacc atctttagga tcatgagcca caacttttaa atttggtgct aagtccttga
     3361 tgctctggtg gtggaaggag ttgatatgag agatttctcc atagatttct tggagaacgg
     3421 tatctggttc tgtcaccaag cgttgagttg tgtactcaac agaagaatcc tgccaatggt
     3481 cttcgatatc ttggtacaaa gttccaccca tggcaacgtt aaagagttgg gtaccacggc
     3541 agacagagaa aatgggcttt ttctgtttaa tagcttcctt gatgagggcc agttcgaaga
     3601 tatctctttg aaggtgataa tcatcgctat caatggtttt gggttcgcca tagaattttg
     3661 gatcgacatt ttgcccacct gtcaagatga gcttgtcaat caaactgata tagtggcagg
     3721 ccatttcttg atcaccaatc ggtaggatga taggaatccc tccagcatct ttaacgcctt
     3781 caacaaagcc ttttgctgcg tagctcatca tgatgtcatc atctggatga gttttttcgt
     3841 ttcctgtaat cccaataact ggttttttca taaaatgatt ttcgctttct aatcctcttt
     3901 tcgcatgaag tagaggaggg tttggagttc acttgtcaaa tcgacatact gaacgaccac
     3961 gtcttttggt aaatgcagat ggactggtga aaaactgaga attcctttca caccagcatc
     4021 aaccaagaga ttagcaacct cttgtgactt gacgctggga acagttagga tagcagtctt
     4081 cacatcagca tccttgattt tatccttgat ctgagaaatc ccgtaaatgg gaatcccgtc
     4141 aggagtttgg gtaccgactt caggatggtc gtctaggtca aaggccatga taatcttcat
     4201 cttgttacgt tcgtggaagc ggtagtggag aagggcatgg cccatatttc caataccaac
     4261 cagcatgaca ttggtaatag agttgtcatt gagcaaatcg gcaaaaaatg tcattagttt
     4321 tttgacatca tagccaaaac cacgacgacc aagttcacca aaataggaaa aatcacgacg
     4381 tacggtcgct gaatcaatcc caatggcctc tgcaatttgc ttagagttgg cacgttcaat
     4441 cttttctgca tgaaatctct taaaaattcg atagtagaga gagagtcttt ttgctgtagc
     4501 ttttggaata gcaaactgtt tatctttcac aaaatcacaa cctttctatt cttctatttt
     4561 atagaaacat tgtgaaaaaa tcaacaaaaa taagaaaaaa ctaagaaaaa tcttagtttt
     4621 gatgtaaaaa atctgcatga gatagaaaac ggtagaggtc tccgaccagc ccctgataaa
     4681 cttttttgcc cctaaaagtc agagaagtca cataaagtgt atctggtaag gttacacatc
     4741 ctgacaaagt caacatgaga gcctcatgat cctcatactt gagagtacgc tctacatgat
     4801 agcagtcctt ataggtcagt tcaaacattt tggctctatc tttccgattt tgtaaagaca
     4861 ccacgttcta ccaagctatc catgaggaag tagaattttt cctgatgaat atggtggtct
     4921 tctgatttga aaatatcaac tagacgaagg ccaaacttgt cagtgatatt gattttagcc
     4981 cctgtaagtt ccttgttaat gatgattttg agttggaagc cttcaccgct gtttggcact
     5041 ttttccaaaa ggcgagtcag ttcatagtta ccaaccttag tttcaaaaaa ggtgttatct
     5101 ttgagggtga attttttaac agaagggcta agagtgtaat cgtaacgaca attttttaac
     5161 tgaatgattt tttcaaatgc catatggcta acctccgata atttctttta aggtttttgc
     5221 gagggtttgt aggtcttcaa cggtattttg tggcgacaaa ctgatgcgaa gggattcctt
     5281 caagcgttct gaatttgcgc catacatggc ttcaagaaca tggctggatt ggacaacgcc
     5341 tgcagtacag gctgagccag tagagattga aattccagct aaatctagcc gaaggagtaa
     5401 gaggtcattt ttctgaccag gaaatccaat attgagaaca taagggagat gatgttttcc
     5461 tctattcaag taatactgaa tgccctccag ctctgccaga aaggcagttt ctagattttg
     5521 tacatgttga aaatgttctt cttgtttttc taggtcttct tttagggctg caaccatgcc
     5581 tacgatagca ggcagatttt cagttcctgc acgttttttc tgttcctggt ctccgccatg
     5641 tagataggaa tcaaagtcca tgctagatgc gtagagaaaa ccgattccct taggaccatg
     5701 gaatttgtgg gcagaagcag tgagaaaatc aatgcccaat tcttctgaat gaattgggat
     5761 tttaccaata gcctgaactg catcaacatg ataggcagca gggtgttgct tgagtatttg
     5821 gccaatttca gcgatgggca gtaggtttcc tgtctcatta ttggcaaaca tggtagaaac
     5881 caaaatcgta tcgtcacgta aagccttttg aatttgctgg gctgtgattt cttgattttc
     5941 tggctggata atggttgctt caaacccaaa gtgttgaacc aagtaatcaa ttgtttcaag
     6001 gacagcatgg tgctcgatgg cagttgtgat gatatgtttt ccttgttctt ggtgacgaag
     6061 acagtagcca atgatggtag tattattgcc ttcagtccca ccagaagtga aaaagatatg
     6121 ttgaggtttt gtccttagta actgggctag ttcctgacgg gcttctcgca agagtttgcc
     6181 agcttgacga ccatgaccat gaatactaga aggatttccg tgggtttctt gcataacctt
     6241 ggtcatagct gaaatagcaa ctgctgacat aggagtcgtt gcagcattgt ccaaataaat
     6301 caaagaatca ccttatttct ttttattgta ggcaaagagt gggctgactg gttttctttc
     6361 gtgaatacgg acgatagcat caccaattaa ctcactagca gtgatgtagc atacattttt
     6421 aggagttttt tcttttgttg ctactgaatc agtcacaaga atttctttaa tattagtatt
     6481 gtcaagaagc tcagcagctc cctcgacgaa gagaccgtgg ctagaaacag cataaatttc
     6541 tgtagctcct tcacgttcaa cgattttaga agcttcagag aaggtacgtc ctgtatttaa
     6601 aatatcatca atcaagatag ctttcttacc ttcaacatca ccaataatat aaccttcgtt
     6661 acgagttgca tcgtcttgag ggtagtcgat aatggcgata ggagcatcaa gatattcagc
     6721 caggctacgc gcacgtttga cacctgaatt tttagggcta acgacaacaa catctgaacc
     6781 aagcaatcct ttatcgcagt aatgttttgc gaatagggga acagtgaaaa gattatccac
     6841 tggaatatca aagaaacctt gaacctgaac ggcatgcaaa tcaagagtca ggatacgatc
     6901 aactccagcc ttaaccagca tattggcaac tagttttgct gtaagtggct cacgaggaca
     6961 agcaatgcgg tcttgacgtg catagccaaa atatggaagg acaacgttga tactgtgggc
     7021 acttgcacgc acacaagcat cgaccatgat taacaattcc attaggtggt tgttgacagg
     7081 gaaacttgtt gattggatga tgtaaacatc ataaccacgg acactttctt cgatatttac
     7141 ttggatttct ccgtctgaaa attgacgtga tgatagtttt ccaagtggga caccaacagc
     7201 ttgggcaatt ttttgtgcaa tctcttggtt agagttgagt gcgaaaagtt tcatgttttt
     7261 tctatctgac attatagacc gtcctctgta aactttataa atcctagtta tatttacctt
     7321 acatatatga actgggattt gtgtattttt atcttttcta ttttaccaaa aaatggagat
     7381 tatttcagct atttttcata cttttgacaa atcgaaccaa ttttgaagga gctttttggt
     7441 aggaaatctg atttttctct aaaaattgtc ggaaatcctg tttgccttgc tcatgatttt
     7501 ccacttcaag ctccaattcg taatctgtta tatcaaagta tcggctctga tccagtgcca
     7561 tgagaccaat agctgttttc atttcatagc gaagcgttgt tagacaacca agaacctgcc
     7621 agttcttact ttggatacca tgtttcgcca attcatccag tactagccct tgaggaagtt
     7681 cttccttact cagatagttc tcagcatctt ttagttgtaa tttttggttg tattccatgt
     7741 ttccaacact ctgcgggact ttgagtgtca attcagccca gtcttcaaag gttcgaatgc
     7801 gcatagcgac tttcttttct cgcagttcaa aatcaggcgt gtcgatgtag taatttgttt
     7861 gaagaacagg agtgacacct gtgaactggt cttttagacg attgtattca tcttttttca
     7921 atagtgtttt caattcaatt tctaaatgtt tcatttttct tacctttttt tatcgttgaa
     7981 agcggattta tggtataata agcattgtat ttattgtata tgaatctgga gaaaaaatca
     8041 aagatatttt tgacggataa tatgagaaca agggagaata tatgacctta gaatgggaag
     8101 aatttctaga tccttacatt caagctgttg gtgagttaaa gattaaactt cgtggtattc
     8161 gtaagcaata tcgtaagcaa aataagcatt ctccaattga gtttgtgacc ggtcgagtta
     8221 agccaattga gagcatcaaa gaaaaaatgg ctcgtcgtgg cattacttat gcgaccttgg
     8281 aacacgattt gcaggatatt gctggcttac gtgtgatggt tcagtttgta gatgacgtca
     8341 aggaagtagt ggatattttg cacaagcgtc aggatatgcg aatcatacag gagcgagatt
     8401 acattactca tagaaaagca tcaggctatc gttcctatca tgtggtagta gaatatacgg
     8461 ttgataccat caatggagct aagactattt tggcagaaat tcaaattcgt actttggcca
     8521 tgaatttctg ggcaacgata gaacattctc tcaactacaa gtaccaaggg gatttcccag
     8581 atgagattaa gaagcgactg gaaattacag ctagaatcgc ccatcagttg gatgaagaaa
     8641 tgggtgaaat tcgtgatgat atccaagaag cccaggcact ttttgatcct ttgagtagaa
     8701 aattaaatga cggtgtagga aacagtgacg atacagatga agaatacagg taaacgaatt
     8761 gatctgatag ccaatagaaa accgcagagt caaagggttt tgtatgaatt gcgagatcgt
     8821 ttgaagagaa atcagtttat actcaatgat accaatccgg atattgtcat ttccattggc
     8881 ggggatggta tgctcttgtc ggcctttcat aagtacgaaa atcagcttga caaggtccgc
     8941 tttatcggtc ttcatactgg acatttgggc ttctatacag attatcgtga ttttgagttg
     9001 gacaagctag tgactaattt gcagctagat actggggcaa gggtttctta ccctgttctg
     9061 aatgtgaagg tctttcttga aaatggtgaa gttaagattt tcagagcact caacgaagcc
     9121 agcatccgca ggtctgatcg aaccatggtg gcagatattg taataaatgg tgttcccttt
     9181 gaacgttttc gtggagacgg gctaacagtt tcgacaccga ctggtagtac tgcctataac
     9241 aagtctcttg gcggtgctgt tttacaccct accattgaag ctttgcaatt aacggaaatt
     9301 gccagcctta ataatcgtgt ttatcgaaca ctgggctctt ccattattgt gcctaagaag
     9361 gataagattg aacttattcc aacaagaaac gattatcata ctatttcggt tgacaatagc
     9421 gtttattctt tccgtaatat tgagcgtatt gagtatcaaa tcgaccatca taagattcac
     9481 tttgtcgcga ctcctagcca taccagtttc tggaaccgtg ttaaggatgc ctttatcggt
     9541 gaggtggatg aatgaggttt gaatttatcg cagatgaaca tgtcaaggtt aagaccttct
     9601 taaaaaagca cgaggtttct aagggattgc tggctaagat taagtttcga ggtggagcta
     9661 ttctggtcaa taatcaaccg caaaatgcaa cgtatctatt ggacgttgga gactacgtta
     9721 ccatttacat tcccgctgag gaaggctttg aaaccttgga ggctattgag cttccattag
     9781 atattctcta tgaggatgac cactttctag tcttgaataa accctatgga gtggcttcta
     9841 ttcctagtgt caatcactct aataccattg ccaattttat caagggttac tatgtcaagc
     9901 aaaattatga aaatcagcag gttcacattg ttaccagact agatagggac acttctggct
     9961 tgatgctctt tgccaagcac ggttatgccc atgcacgatt agacaagcag ttgcagaaga
    10021 aatctatcga gaaacgctac tttgctttgg ttaagggaga tggacatttg gagccagaag
    10081 gggaaattat tgctccgatt gcgcgtgatg aagattccat tattaccaga cgagtggcta
    10141 aaggcggaaa gtatgcccat acttcataca agattgtagc ttcttatgga aatattcact
    10201 tggtctatat tcacctgcac actggtcgaa cccatcaaat ccgagtccat ttttctcata
    10261 tcggttttcc tttgctggga gatgatttgt atggtggtag tctggaagat ggtattcaac
    10321 gtcaggctct gcattgccat tacctatcct tttatcatcc atttttagag caagacttgc
    10381 agttagaaag tcccttgccg gatgatttca gtaaccttat tacccagtta tcaactaata
    10441 ctctataaaa actgtctcag agtataatta ttatcttaaa ggagaaaact catggaagtt
    10501 tttgaaagtc tcaaagccaa ccttgttggt aaaaatgctc gtatcgttct ccctgaaggg
    10561 gaagagcctc gtattcttca agcaacaaaa cgcttagtaa aagaaacaga agtgattcct
    10621 gttttgcttg gaaatcctga aaaaattaaa atttatcttg aaattgaagg aatcatggat
    10681 ggttatgagg tcatcgaccc tcaacattat cctcaatttg aagaaatggt ttctgccttg
    10741 gtggagcgtc gcaagggcaa aatgactgaa gaagatgtac gcaaggtttt ggttgaagat
    10801 gtcaactact ttggtgtgat gttggtttac ttgggcttgg ttgatggaat ggtgtcagga
    10861 gcgattcact caacagcttc aacagttcgc ccagctctac aaatcatcaa aactcgtcca
    10921 aatgtaactc gtacttcagg agccttcctc atggttcgtg gtacggaacg ttacctattt
    10981 ggagactgtg ccattaatat caatccagat gcagaagcct tggctgaaat tgccatcaac
    11041 tcagcaatca cagctaagat gtttggcatc gaacctaaaa ttgccatgtt gagctattct
    11101 actaaaggtt cagggtttgg tgaaagcgtt gataaggtcg ttgaagcaac taaaattgct
    11161 cacgacttgc gtcctgacct tgaaatcgat ggtgagttgc aatttgatgc ggcctttgtt
    11221 cccgaaactg cagctctgaa agctccggga agtacagtag ctggtcaagc aaatgtcttc
    11281 atcttcccag gtatcgaggc aggaaatatc ggttacaaga tggctgaacg cctgggtggc
    11341 tttgcggctg taggacctgt tttgcaaggt ttaaacaagc cagttaatga tctttctcgt
    11401 ggatgtaatg cagatgatgt ttacaagttg accctcatca cagcagctca agcagttcat
    11461 caatagtgaa aactataaag tgatatacta tgctatactg tagttatgaa actatgtacg
    11521 aaaagcactg ccattaattc ctgagaacta aattactgat tggtgtcaaa aaggaaaact
    11581 tccaagcgat gatatcctgt ctatacacga cctatagaaa tctgtaatat acatatccgt
    11641 aaaacgataa attccctttt tgattttaaa tgagtatgaa aagagaattt tttggctctt
    11701 tgtcaactgt agtgggttga agaaaagcta agctcgagaa aggacaaatt tcgtcctttc
    11761 ttttttgatg ttcaaagcga taaaaatc
//
LOCUS       DAVKGU010000058        11786 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKGU010000058 DAVKGU010000000
VERSION     DAVKGU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.058"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(208..1455)
                     /locus_tag="VW077_001460"
     CDS             complement(208..1455)
                     /locus_tag="VW077_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9455460.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            1635..1856
                     /locus_tag="VW077_001461"
     CDS             1635..1856
                     /locus_tag="VW077_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455461.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            1973..2470
                     /locus_tag="VW077_001462"
     CDS             1973..2470
                     /locus_tag="VW077_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEU9455462.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            2445..2951
                     /locus_tag="VW077_001463"
     CDS             2445..2951
                     /locus_tag="VW077_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU9455463.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            2935..5382
                     /locus_tag="VW077_001464"
     CDS             2935..5382
                     /locus_tag="VW077_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9455464.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            5385..7562
                     /locus_tag="VW077_001465"
     CDS             5385..7562
                     /locus_tag="VW077_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9455465.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMKQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            7559..8560
                     /locus_tag="VW077_001466"
     CDS             7559..8560
                     /locus_tag="VW077_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEU9455466.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            8557..9489
                     /locus_tag="VW077_001467"
     CDS             8557..9489
                     /locus_tag="VW077_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU9455467.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            9734..9850
                     /locus_tag="VW077_001468"
     CDS             9734..9850
                     /locus_tag="VW077_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEU9455468.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9866..11785
                     /gene="tet(M)"
                     /locus_tag="VW077_001469"
     CDS             9866..11785
                     /gene="tet(M)"
                     /locus_tag="VW077_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEU9455469.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         3733 a         2134 c         2474 g         3445 t
ORIGIN      
        1 aatggagata aattattaga tatactactg acagcttcca agaagctaaa gaggtcccta
       61 gcgcctacgg ggaatttgta tcgataaggg gtacaaattc ccactaagcg ctcgggaccc
      121 cttgtaggaa aatgtcctaa gtgggatatc tgtcaactgg tatggttgac taaaaatact
      181 tcctacgaaa atgtaggggg tattttttta cgaaaaaata caatcgattc ttaaaaagaa
      241 aaatttttga ttggcaaaac cataacaagt tcgttttagg gttttgattt tgcgattgat
      301 gccttctaaa ggaccattag agtattcaaa tttagcgcta tttaagacat attttctgtt
      361 ttgacgaagg gtttgaatag cagtatccat ttctgtattg gttttttggt agtctaagat
      421 ggttgactct agtaattcac tattgcgctc gtttagggct ttcgtgatat cttggtaagt
      481 ttggtatact tcagcgaact tggaaaattt actagtaatg agatcaacag cattttggcg
      541 agtcatatat tgtttaacgc cgcgaagaaa aactacttct tcagggtgaa gatcttcagc
      601 ttttttatgg aatagcttcc aatgtgactt cataatttta tattcttggc tctgtttatc
      661 aagttgcttt aggatagaga tacgacaatt gtccaaagcg cgaccagcta attgtacaag
      721 gtggaagcga tcaataatga tattggcatt agggaaaagg cgatagataa aactttgata
      781 ttgagcattt aaatcaatta caactgattg aacgcattcg cgttcggctt ttgaataacg
      841 actttcaaaa taatcaacaa tggtaggtga tagacgatcc tgtaactttg tgacaatttg
      901 gtgggtttca gcgtcacaac agataaagga catcacagac ttaattgaac gaaactcgtc
      961 aaaacataga tgcttaggca acttagccac acgatagtgt ggttccatgc gctctaagat
     1021 tgttcgacga acactgctag gagagcagtg acacatttca gcaataagct gaccagataa
     1081 gcctttacga gctaaaagca tgatttgatt tttgagatca ctggataagg tttgattttc
     1141 tttggttaaa ttagtaatag caccaaaagt agtatggcat gatttacatt tatagcgttg
     1201 tttacgaagc tctagttcat atcttctccc atttaaactt gccagtcgta catgagtttt
     1261 gcgaaagcca tccttattaa ctgtgggaaa gccacagtta cgacaacgat taatcggata
     1321 agaaagagta gctgttatta gcgttatata ctctttaaca gaatcgttgt tgtgttcagc
     1381 ttcttcaaca gaaataattt taatattttt atctttaatt ccaagaatat ttaggataga
     1441 atcattatgg gacatttgtt taaccttctt tcatgatttt tgtggtgaat tgattgtata
     1501 acgaggggac agcaaatgtc ctcttttttg tataaaaaaa tctggcatgg aatctctatc
     1561 cataccagaa agtgtatacc ccaaaaaaat aactcaaata caaattcatt gaatatagag
     1621 aggagaacat ttttatgaat tttggacaaa acctttataa ctggtttcta tcaaacgctc
     1681 aatcactggt gcttttagca atcgttgtga ttggcttgta tcttggcttc aagcgtgagt
     1741 ttagcaaact gattggcttt ttaattattg cgattattgc ggttggctta gtcttcaacg
     1801 ctgctggagt aaaagacatt ttactagagc tattcaatcg cattattggt gcttaaataa
     1861 aaccgttctt ttgtggaata taagtggttt tcttatgttc cgcaaaggaa tggtacacca
     1921 aacgaagtgc ggtagggatt tttgaatctc tacaaagaaa ggacgtgaat atatggacga
     1981 tatgcaagtc tatattgcga atttaggcaa atacaatgag ggcgaattgg tcggtgcgtg
     2041 gtttaccttt cccattgact ttgaggaagt caaagagaaa atcggcttga atgatgaata
     2101 tgaggaatac gccattcatg actacgagtt accctttacg gttgacgaat acacttccat
     2161 tggcgaactc aatcgactat gggaaatggt atcggaatta cccgaagaat tacaatcgga
     2221 gctatctgct ctgctcactc atttttcaag cattgaagaa ctaagcgaac atcaagagga
     2281 tattatcatt cattccgatt gtgatgatat gtatgacgtg gcacgctact acattgaaga
     2341 aacgggtgct ttaggcgaag taccagctag tcttcaaaac tatattgatt atcaagccta
     2401 tggtcgggat ttagaccttt caggaacgtt tatctcaacc aatcatggga tttttgaaat
     2461 cgtctattaa atctgtcggt acattactac tggcagattt tctattttac ggggtggctc
     2521 aatcagctac ccctattttt tatgaaagga ttgattacat gaagaaaata cgaagctata
     2581 ccagtatctg gtctgtggaa aaggtactgt attctatcaa tgattttaga cttccgtttc
     2641 ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtg atgatacttg
     2701 gcaacttgcc ccctctttcc atgatagagg gagcatttct caaatacttt gggattcctg
     2761 tggctttcac atggtttatg tctacaaaaa cttttgatgg taaaaagcct tatggatttt
     2821 tgaagtctgt cattgcttat gcactgcgac caaagctgac ctatgcagga aaaaaagtaa
     2881 cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa ttttatggca
     2941 tatccaatta aatacattga aaacaatctc gtctggaata aagacgggga atgttatgct
     3001 tactatgagc ttgttcctta caattactca tttctaagtc cagaacagaa aatacaagtg
     3061 catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaaaattca tgctttacaa
     3121 atcagtacag aatccagcat acgttctgca caagagcgtt ccaaaaatga agtcactggc
     3181 aagctcaaag cggttgccta tgacaaaatc gaccaacaga cagacgcttt aatatccatg
     3241 attggcgaaa atcaagtgaa ctaccgtttc tttatcggct ttaagttgct tctcaacgat
     3301 caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgctttgtc tgattttgtc
     3361 tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga tgaaatcctg
     3421 cgttttcaga agatggaaaa gctcttagaa aataaaatct ctcgtcgttt caaaatccgc
     3481 aggttagata aggacgactt cggctatctg attgaacacc tttacggaca gacaggcact
     3541 gcctatgaag agtatgagta ccatctatca aagaaaaagc tggataatga aacgctgatt
     3601 aaatactatg acttgattaa gcctactcgc tgtttggtgg aagaaaaaca gcgatatttg
     3661 aaaatccagc aggaagatga aaccgtctat gtagcttact ttaccattaa cagcattgtc
     3721 ggagaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca atttacattc
     3781 ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct atctactgtc
     3841 cgcaataaaa agaaagaact gaaagacttg gataaccacg cttggcaaag tgataatgaa
     3901 accagctcca atgtggcgga agctctggaa agtgtgaatg agctggaaac caatttagac
     3961 caaagcaagg aatctatgta caagctgtct tatgtggtaa gggtatcagc aaatgatctt
     4021 gacgaactca aacgtcgttg taatgaagtg aaagattttt atgacgattt aagcgtaaaa
     4081 ctggtacgac catttgggga tatgctcggc ttacatgaag aatttttacc tgccagcaag
     4141 cgttatatga atgattatat tcaatacgtg acctctgatt tcctcgctgg tttaggtttt
     4201 ggtgctactc aaatgctggg ggaaaatgag gggatttatg ttggctacag cttagatact
     4261 ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg ttcagtaacc
     4321 aatgcgttag cgtcggcttt tgttggttcg ctgggtggtg gtaaatcctt tgcgaataac
     4381 cttatcgtct attatgcggt gctttatggg gcacaagcag tgattgtaga cccaaaagca
     4441 gaacgtggca gatggaaaga aaccttgcca gagatttccc atgaaatcaa tatcgtcact
     4501 ctgacttctg atgagaaaaa caaaggctta cttgaccctt atgtgattat gaaaaatccc
     4561 aaagattctg aatcactggc tattgatatt ctgacattcc ttacggggat ttcctctcgt
     4621 gatggggaac gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa tagtgaagta
     4681 cgagggttga tgaaagtgat tgaggaatta cgggttgaga atacgccact aagtaccagt
     4741 atagccgacc atatcgaaag ttttacagac tatgactttg cacatttatt attcagtaat
     4801 ggttatgtgg agcagtctat cagcttagaa aaacaactga acattataca ggttgcggac
     4861 ttggtacttc ccgacaagga aacttccttt gaggaatata ccactatgga gcttttatcc
     4921 gttgctatgc tgattgtcat tagtaccttt gctttagact ttatccatac agaccgaagc
     4981 attttcaaga ttgtagattt agacgaagca tggagctttt tacaggtagc acaaggaaaa
     5041 acactatcta tgaagctggt tcgggctggt cgtgctatga acgctggggt atatttcgtg
     5101 acccaaaata cagacgacct cttagatgaa aaactgaaaa ataacctcgg cttaaaattt
     5161 gcatttcgtt ccactgacct taacgagatt aaaaagacct tagccttttt tggtgtagac
     5221 ccagaggacg aaaacaatca gaagcgattg cgtgatttgg aaaacgggca atgccttatc
     5281 agtgatttat atggtcgtgt cggtgtgata cagttccacc ctgtatttga agaactgctc
     5341 catgcctttg ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa ccatcaatag
     5401 taaacagaat aaaatcaaac tggacgctga aacgtctagg taaagtggca atgacagtgg
     5461 ctttcacact tgtgattgcc atttttcttt tagccatgct gggaacggtg gttcaagctg
     5521 cgggcttggt agatgatacg gtcaatgtgg caaatgaata cagccgatac ccacttgaaa
     5581 actatcaact ggatttttat gtggataata gctggggctg gcttccgtgg aactggtcgg
     5641 acgggattgg aaaacaggtc atgtatggac tatatgccat taccaatttt atttggacaa
     5701 tcagtttgta tgtttccaat gcgacaggtt acttagtaca ggaagcctat tccttagact
     5761 tcatttccgc tacagcagat tccattggta agaatatgca gaccttagct ggtgtgagtg
     5821 caaacggatt ttcaacagag ggtttctatg ttggattcct cttactcttg attttggttc
     5881 ttggggttta tgttgcctat acgggactga taaagagaga aaccacaaag gcaattcatg
     5941 ccattatgaa ttttgtgctg gtgtttatcc tatcggcttc ctttattgcc tacgctcccg
     6001 actacattaa aaaaatcaat gacttttcat cagacatcag taatgccagt ttatcacttg
     6061 gcacgaagat tgtcatgccc cattccgata gtcaaggcaa ggacagcgtg gacttaatca
     6121 gagatagcct gttttccata caggttcagc aaccgtggct actgcttcaa tacaacagtt
     6181 cagacattga aagtatcggt attgaccgtg tggaaagcct gctctccacc agcccagatt
     6241 ccaacaatgg cgaagacaga gaaaaaattg ttgcggaaga aattgaagac agaagcaata
     6301 ccaatctaac cattacaaag accattaacc gtttaggtac agtcttcttc ctatttgtct
     6361 tcaatattgg gatttccata tttgtattcc tattaacagg aatcatgatt ttctcgcagg
     6421 tactttttat catctatgct atgtttctgc ctgtgagctt tattttaagc atgattccat
     6481 catttgatgg tatgtcaaaa cgagccataa caaagctctt taataccatt ttgacacgag
     6541 ctggaatcac attgattatt acgacagcat ttagtatttc aaccatgctc tataccttat
     6601 cggctggtta tccgttcttt ttgattgctt ttctacagat tgtgaccttt gcaggaatct
     6661 acttcaagct gggcgattta atgagtatgt tttctctaca gagtaacgat tctcaaagtg
     6721 tgggaagtcg tgtgatgaga aaacctcgta tgcttatgca cgctcacatg caccgtctac
     6781 agcggaaact tggacgttcc atgactactc taggggctgg gtctgccatt gttacaggta
     6841 aaaaaggaca gtcgggttcg gggagttctg caaggacaca agcagatcac tcccgaccag
     6901 acggaaagga aaaatcaaca cttggaaaac gtatcggtca aaccatcggt acagtagctg
     6961 ataccaaaga cagaatggta gacactgcta gtggtttgaa agaacaggtt aaagatttgc
     7021 cgaccaatgc aagatatgca gtatatcaag gaaaatccaa agtaaaagag aatgtccgtg
     7081 atttaaccag tagtatttct caaaccaaag cggacagagc cagtggacgc aaggaacagc
     7141 aggaacaaag gcgaaaaacc attgcgaagc gtcgctctga aatgaaacag gtcaaacaga
     7201 aaaaacagcc tgcttcttct gttcatgaaa gaccgactac aagacaagaa caatatcatg
     7261 atgaacagac ctcaaaacag tctaatattc agacttcata taaggaatct caacaagcca
     7321 aacaagagcg tccagcagtt aagtccgatt tttcaagtcc aaaagtggaa cgccaaggca
     7381 ataccgttca agaaaaaacc gttcaaaagc cagcaacttc aaccactaca gcagatagaa
     7441 cttcacaacg tccaatcaca aaagaacgtc cgtctactgt tcaaagagta ccactacaaa
     7501 atacaagaag tagaccacca atcaaaaccg ccaccattaa gaaagtcggt aagaaaccat
     7561 gaagttgaaa actttagtga ttggtggttc tggattattc ttgatggtct tctcactgct
     7621 tctgtttgtt gccattttat tttcagatga acaggacagc ggaatttcca atattcatta
     7681 tggaggtgtg aatgtttccg cagaagtgct ggctcataag cctatggtag aaaaatatgc
     7741 caaagaatat ggcgttgaag aatatgtcaa catacttctt gcgattatac aggtggaatc
     7801 gggcggtact gcggaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     7861 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgaat tattagccag
     7921 tagcgaaagg ctcagtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     7981 tttcttaggg tatgtggcta atcgtggaaa taaatatacc tttgaactgg ctcaaagttt
     8041 ctcaaaagag tattcaggtg gcgaaaaagt gtcttacccc aatcccatag ccatacctat
     8101 caatgggggc tggcgataca actatggcaa tatgttttat gtgcaactgg taacgcagta
     8161 tcttgtcaca acagagtttg atgatgatac ggtacaagcc atcatggacg aagcactgaa
     8221 atatgagggc tggcgatacg tttacggtgg agcttccccg actacttctt ttgattgtag
     8281 cggactgaca caatggacgt atggaaaagc tggaattaac ttaccacgaa ccgcacaaca
     8341 gcaatatgat gtgacccagc atatcccact atcggaagca caagctggcg atttggtttt
     8401 ctttcattct acctataacg ctggctctta tattactcat gttgggatat accttggcaa
     8461 taaccgtatg tttcatgcag gcgacccaat cggttatgcc gacttaacaa gcccctactg
     8521 gcaacagcat ttagtgggag caggacgaat caaacaatga gaaaggaaga tttaatgatg
     8581 aaatttagaa aaaatcagaa taaagaaaaa cagataccaa aggaaaagaa acctcgtgtc
     8641 tataaggtca atcctcataa aaaggttgtg attgccttgt gggtactttt agggcttagt
     8701 ttcagctttg cgatattcaa gcactttaca gctatagata ctcatactat tcacgaaaca
     8761 actatcatag aaaaggaata cgttgatact catcatgtag aaaattttgt agagaacttt
     8821 gcgaaagtct actattcatg ggagcaatcc gataagtcca ttgataatcg aatggaaagt
     8881 ctaaaaggct atctgacaga tgaacttcaa gctctcaatg ttgatacagt acgcaaagat
     8941 attcctgtat cgtcttctgt aagaggattt cagatatgga cggtagagcc aactggcgac
     9001 aatgagttta atgtaaccta cagtgtagac cagctcatta cagagggaga aaatacaaag
     9061 accgtccact ctgcttatat agtgagtgtc tatgtagatg gttctggaaa tatggtactg
     9121 gttaagaatc cgaccattac caacatacct aagaaatcaa gttataaacc aaaagccatt
     9181 gaaagtgagg ggacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     9241 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     9301 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tattcacaat
     9361 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     9421 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     9481 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     9541 taaaaaattg gagattcctt tacaaatatg ctcttatgtg ctattattta agtatctatt
     9601 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     9661 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
     9721 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     9781 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     9841 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     9901 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     9961 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
    10021 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
    10081 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
    10141 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
    10201 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
    10261 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
    10321 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
    10381 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
    10441 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgttc
    10501 tctgttccct ctttatcatg gaagtgcaaa aagtaatata gggattgata accttataga
    10561 agtgattacg aataaatttt attcatcaac acatcgaggt cagtctgaac tttgcggaaa
    10621 agttttcaaa attgagtatt cggaaaaaag acagcgtctt gcatatatac gtctttatag
    10681 tggcgtactg catttgcgag attcggttag aatatcggaa aaggaaaaaa taaaaattac
    10741 agaaatgtat acttcaataa atggtgaatt atgtaaaatc gataaggctt attccgggga
    10801 aattgttatt ttgcagaatg agtttttgaa gttaaatagt gttcttggag atacaaagct
    10861 attgccacag agagagagaa ttgaaaatcc cctccctctg ctgcaaacga ctgttgaacc
    10921 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
    10981 cccgcttctg cgatattatg tggattctgc gacacatgaa atcatacttt ctttcttagg
    11041 gaaagtacaa atggaagtga cttgtgctct gctgcaagaa aagtatcatg tggagataga
    11101 aataaaagag cctacagtca tttatatgga aagaccgtta aaaaaagcag agtataccat
    11161 tcacatcgaa gttccaccga atcctttctg ggcttccatt ggtctatctg tagcacagct
    11221 tccattaggg agcggagtac agtatgagag ctcggtttct cttggatact taaatcaatc
    11281 gtttcaaaat gcagttatgg aggggatacg ctatggctgt gaacaaggat tgtatggttg
    11341 gaatgtgacg gactgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
    11401 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
    11461 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
    11521 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
    11581 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
    11641 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
    11701 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
    11761 atatatgttc aataaaataa cttagt
//
LOCUS       DAVKGU010000059        11125 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKGU010000059 DAVKGU010000000
VERSION     DAVKGU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11125
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.059"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(47..1936)
                     /locus_tag="VW077_001470"
     CDS             complement(47..1936)
                     /locus_tag="VW077_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9455470.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSSFERDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2074..3639
                     /locus_tag="VW077_001471"
     CDS             2074..3639
                     /locus_tag="VW077_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9455471.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3639..4379
                     /locus_tag="VW077_001472"
     CDS             3639..4379
                     /locus_tag="VW077_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9455472.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4488..4703)
                     /locus_tag="VW077_001473"
     CDS             complement(4488..4703)
                     /locus_tag="VW077_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455473.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5574..6824)
                     /gene="ilvA"
                     /locus_tag="VW077_001474"
     CDS             complement(5574..6824)
                     /gene="ilvA"
                     /locus_tag="VW077_001474"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9455474.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6874..7230)
                     /locus_tag="VW077_001475"
     CDS             complement(6874..7230)
                     /locus_tag="VW077_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9455475.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7468..7704)
                     /locus_tag="VW077_001476"
     CDS             complement(7468..7704)
                     /locus_tag="VW077_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455476.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(7811..8833)
                     /gene="ilvC"
                     /locus_tag="VW077_001477"
     CDS             complement(7811..8833)
                     /gene="ilvC"
                     /locus_tag="VW077_001477"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9455477.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8899..9375)
                     /gene="ilvN"
                     /locus_tag="VW077_001478"
     CDS             complement(8899..9375)
                     /gene="ilvN"
                     /locus_tag="VW077_001478"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9455478.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9368..11068)
                     /locus_tag="VW077_001479"
     CDS             complement(9368..11068)
                     /locus_tag="VW077_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9455479.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
BASE COUNT         3359 a         2546 c         1918 g         3302 t
ORIGIN      
        1 tctatcttcc attatatcat actttttcag tctatggtag ctctctttaa aaggctccga
       61 tactgccgcc acctccgcct ccagagaagc caccgccaga acttccactt ccagaagata
      121 cggagtaggt acttgctgta tttgcgacac tagcataatg gctcatttgc gcgcttgaat
      181 gataaaacat actgtgccag ccataagcta catagagatt gatatctgga ttttcaactt
      241 gaatctgatg aaccttcatc aaatgactaa ccttgtccgc atagccaaat aaggttgcat
      301 agaccaagag acgattccaa acaacaatac tttccaattc agccttatcc agtcgtgcaa
      361 tctcacgtaa catattttca aaactggtcc agagatagta gacctcagct cccgcttcat
      421 ttagaacacc atcacgatta tctagtcgaa gcttccaata atagaaaaca gacaaaacta
      481 accctagaaa accaagtatt ggcaaaggga ggtaaagata gccatgaacg tctaaactgt
      541 acaagaacaa accaaatccg ataaataggg gcaggatagt caaggcaccc atacccactt
      601 gcaaggcctt ttccccacca gttaaaggac gataataatc tgggagcccc cagaaggaaa
      661 ctcgttttct cactccttct tgcatctggt tcaatacctc ttcaaaagaa gatttgagtt
      721 gaagccctct tgcttgaatc cgtttttcat cagaaacttt ggctctacga taaagactat
      781 cagatacctt gtaatccgca aacaaattgg aaagagtttc ttctttttta cctgaaaaag
      841 ctagatttag gcagtctctc tcaaagcttg acaaaccatc ttcttttact agcctcaaac
      901 caactgcatc tccttctgaa ataatagaaa cattcccacg gtccatcaca tctagcaagg
      961 tagcttgaat aagttggtca aaagtgaatt tgccagcccc cttgactaag ggactcactt
     1021 cctccaagga ggtcgagtag acagcctctg ataaaaccat aggctctaat tccattggtg
     1081 gttcatagag acgatgattt ttggcatatt tgactgaagg agtggtcttt cttctataaa
     1141 taaaatagaa gcagacactc aataacaagg agatagaaag gatcgaaggg aggacccaag
     1201 taacaagttg tttactctga tctttttctc taacaatcga gtcttctatc ttattaaact
     1261 cttctaaacg attccctttc aatccctgat ccctagcgct agcaaaatcg gtccgaggcc
     1321 agtaggcatg caactcaact ccacgcttag ccggaagatt gtctaaacgg atagtataat
     1381 caaggttact cttttcaatc gttccctctc taaaaagttt ccctgtatgg aaaaagagtt
     1441 tttcagcccc cttgtctccc cttacatgaa attcaaactt ttcaatagac tctgaactat
     1501 ctgtcagagg ttgccaattt aattcagcga tatcatcata aaggaaaagt aaatttttta
     1561 agttccagac gaggtcaact tcaactatgt cgccctcctg acctggatta tagactctca
     1621 cagtataacc atccgcttct tctgttactt cgctagtcac atctgctagt tctgcaccgt
     1681 ttttcgcggc ctgaaccttt ggatgagggt caatgtcaaa cccgctaggc atcttaccag
     1741 cacgtccaag tcccacgatt tgtcccttaa agtcctcctc aaactggtaa actatcttct
     1801 gtctaaactc tgccgtatta tctgcatgaa tatacaaatc cccttgataa gagtttatct
     1861 tgaaatcaat ggcaaaaaca gagagtggaa gaaggcaaaa caagcctaac accagtaaga
     1921 aaaaagtttt tttcatcaac taagccccct ttttatcttt cttatcatta tagcattttt
     1981 tcgtaagtaa aggcttactt atgttgaaaa attacgcatt tttcgataaa ataggagaca
     2041 gttatttttt aatagatcag aataggagaa atcatgagaa aaatatactt atctattttc
     2101 acaagtctct tgctgatgct aggacttgtc aatgttgctc aagccgatga atatttacgc
     2161 atcgggatgg aagcagcata tgctcccttt aactggaccc aggatgatga tagcaacgga
     2221 gctgtcaaaa tcgatgggac caatcagtat gccaacggat acgatgttca aatcgccaag
     2281 aaaatcgcta aggacttagg taaagaacct ttggttgtta aaaccaagtg ggaaggtcta
     2341 gtccctgccc ttacttctgg taagattgac atgattatcg caggtatgag tccaactgca
     2401 gaacgcaaac aagaaattgc cttttcgagc agttactaca ctagcgaacc agttttgctt
     2461 gtcaaaaaag attctgccta cgcaagtgct aaatctttgg atgactttaa cggtgcaaaa
     2521 atcacttctc aacaaggggt ctacctctac gacttgattg cacaaatccc aggtgctaaa
     2581 aaagaaacag ccatgggaga cttcgctcaa atgcgacaag ctcttgaggc tggtgtcatt
     2641 gatgcttatg tttctgaacg tccagaagca ctgactgctg aagctgcgaa ctctaagttc
     2701 aagatgattc aagtagaacc tggtttcaaa actggggaag aagatacagc tatcgctatc
     2761 gggcttcgta aaaatgacaa tcgtattagc caaatcaatg ccagcattga aaccatttca
     2821 aaagatgacc aagttgcctt gatggatcgt atgatcaagg aacaacctgc cgaagctaca
     2881 acaactgaag agactagcag tagtttcttt agccaagtcg ctaaaattct ttctgaaaac
     2941 tggcaacaac tcttgcgtgg tgctggtatc actcttttaa tctctatcgt cggaaccatc
     3001 ataggtctca ttattggact tgccattggt gtcttccgta ctgctcctct ctctgaaaac
     3061 aaagccattt atggccttca aaaactagtc ggctgggttc tcaatgtcta cattgaaatt
     3121 ttccgtggta cgccaatgat tgttcaatcg atggttatct actatggaac tgcccaagct
     3181 ttcgggatca accttgaccg tacactggct gctatcttca tcgtttcaat caataccggt
     3241 gcctacatga ctgaaatcgt ccgtggtggt atcctagcag ttgacaaggg acaatttgaa
     3301 gctgcgactg ctcttggtat gacccataac cagaccatgc gtaagattgt cctacctcag
     3361 gtagtccgta acatcctacc tgcaactggt aatgaatttg tcatcaatat caaagataca
     3421 tctgtattga acgttatctc tgttgtcgaa ctttatttct caggaaatac cgtggcaaca
     3481 caaacctatc aatacttcca gacatttaca atcatcgccg tgatttactt tgtcctcacc
     3541 ttcaccgtaa cacgtatcct acgctttatc gagcgcagaa tggacatgga tacctacact
     3601 acaggtgcta accaaatgca aacggaggat ttgaaataat gacacaagca atccttgaaa
     3661 ttaaacacct caaaaaatcc tatggacaaa acgaagtgct aaaagacatt tcactcactg
     3721 tccacaaggg agaggtcatc tctatcatcg gaagctctgg aagcggaaaa tcgaccttcc
     3781 tacgctccat taacctactt gaaacaccaa ctgatggaca aatcctttat catggacaaa
     3841 acgtcctcga aaaaggctat gacctcacgc aataccgtga aaagttgggg atggttttcc
     3901 aatcctttaa cctctttgaa aatctcaatg ttcttgaaaa cacaatcgtc gctcagacaa
     3961 ctgtcctaaa acgcgaacgc acagaagctg aaaagattgc caaagaaaac ctggaaaagg
     4021 tcggcatggg agaacgctac tggcaagcca aaccaaaaca actctcaggt ggtcaaaaac
     4081 aacgtgtggc catcgctcgt gccctctcca tgaatccgga cgccattctc tttgatgaac
     4141 caacatcagc tctcgatcca gaaatggttg gagaagtcct caaaatcatg caggacctgg
     4201 ctcaggaagg cttgaccatg attgtcgtaa cccatgaaat ggaatttgcc cgtgatgtct
     4261 ctcaccgtgt tatctttatg gataagggcg tgatcgctga agaaggtaaa ccagaagacc
     4321 tcttcaccaa tcctaaagaa gaccgaacaa aagagttcct tcaacgctat ctcaaataaa
     4381 aagaaaaggc tgcatcaacc gtgcagtctt tttgctgtcc tcaaaatgaa aaggcagatt
     4441 ctctatctct actacttgaa ggtcaagaat ccgccgttat ccaaagatta atcttcaaat
     4501 ttttcataat ttttttcata gaaatcaatt aggcctaggg ctgtttcaaa tgaaatattt
     4561 tttacttttg cacgaccctg cgccagagca atgatagaca tttcacgcgc attcgtttct
     4621 ttagagatac ggtagcctgt gattttctta tcacgaaccc agccaacaac tgattctact
     4681 ttttcaaagt ttgacttagc catgtttttc tcctattttc ttctttataa tatttaattg
     4741 tatacgtttt tactactttt gtcaataaaa aaaacatata ttcaacgaaa ataattagtt
     4801 cagaattttc ttacttcctt tttaaatccg ataatatata ggttttgcta gcctctacac
     4861 cataaatatt tgctaataaa actactgtat tattaccctc ttaaggtgcg aaattattca
     4921 agttcttaaa aaatgcttct ctatttccgt aagatagctt atttcttcga tttaagaact
     4981 gaagatgata gttctaacct ttagaaatcc aagccttcac tagctcaaaa actcctatct
     5041 ttcttcctca ataaatcatc tgagagatag gtattcctta tatccttcaa attcatcact
     5101 cctttggata ttgaggtgct agcctagaag cagacacccg attatctaaa ccatccttaa
     5161 tagagatgac aagacagata agctcagtta attacttata tatcttttta atatggtata
     5221 tttgtcaaca aaaagaaatc aaatcatacc ttatactcaa tgaaaatcaa agagcaaact
     5281 aggaagctag ccgcaagttg ctcaaaacac tgttttgagg ttgcagatag agctaacgtg
     5341 gtttgaagag attttcgaag agtattaaag acagtgtgac tatgcttgtc aatagatagg
     5401 aaatcaaaat gaaggatgga aatctgagcc aaacatcata agtgctataa tgaacagaag
     5461 taagaattgc acgataagtt ttctaaaacc ataggaatcg tcccctttct tcatctttat
     5521 tatagcacat atacatcagt tgtacaaata atatgatatt tttttattag tcctcagaca
     5581 agcatattat aaagcgtttc attaccattt aagttaatat aagctggatc aaaaccttcc
     5641 attctacgaa tcaaacctgc ataatcatgc ttatctgcta aagcgatccc aattaatact
     5701 gggcctgttc ccttgctagc tcgtttgata tactcaaaac gtgtgatatc atcatttggc
     5761 cccaggatat catttacaaa ctcacgcaaa gctcctggac gttgtgggaa attgaccaca
     5821 aagtaatgtt tgataccatc ataaatcaag gcacgctctt ccatttctgg catacggttg
     5881 atatcattat ttcctccaga aatgatacaa caaatggttt tccccttaat atattcagct
     5941 aaaacctcta aagaggcgat actagccgct ccagcaggtt ctgcgactat cccttgctta
     6001 gagtaaaggt caatcaaggt ttcagaaatc aatccctcat cgacacctac caaagtttta
     6061 atatgttgac gagttgcttc ataggtcaac tgacctacct tttgcacagc aatcccatcc
     6121 gcaaatttat caatttcctt gagtttgact ggacctccag cctcaaaggc agctttcatg
     6181 gaacgcgctc cattcgcctc tactccgata acctcaatct ctggacttgt ttccttgata
     6241 taggtagaaa ccccggcaat gagaccgcca ccaccaacag gaaccaagac agcatcaaaa
     6301 tcaatcgatt cttttcgagc ttcttctaaa atctcataag caactgttcc ttgacctgct
     6361 tgaacatgag catcatcaaa aggatcaata aaggtacgat tttcagagac tgtaaattct
     6421 tgagctgctt tggctgaggc atcaaaggta tctccaacta gtttaatagt tacaaaatcc
     6481 ccaccaaaaa agcgaacctg accaatcttt tgttgtggcg tagtaatagg cataaagata
     6541 gtagcaggaa ttttcatttc attacaagta taggctactc cctgcgcatg atttcccgca
     6601 gaagcgcaga ctaccccacg ttcacgttct tccttgctga gctgggaaat ggcataatag
     6661 gcaccacgaa ttttaaagga gcgaacacgc tgggcatttt cttttttcaa ataaatctta
     6721 gcaccatact tctccgataa ataatgatcg taatccagtg gagtattcac aaccacaccg
     6781 ttcaagacct tgtgagcctt gattatatct tttgaactta acatataaaa cctctttttc
     6841 tataaaaaac agtcactaat gactgttttt tagttattca tctccgaaaa cttcagtaac
     6901 actttcatgg tatttatgag ccatacgaat cgaaaactct ttaggaagtt cataacgatt
     6961 tgtggaaatc caatcatata accattctac caaactttct tcactatcat tacgatggaa
     7021 gataaaatag tttaaaattt ctttatattc tacatcattc aaatccaacg tgtctacaaa
     7081 ttgactacga ggaacaaaat tagaatctaa ttttcttagt tgatctgtca attctcccaa
     7141 ttgtaaatcc aactgaaaaa atagtttttg cagttgccta tcagtttcat cacatcctgt
     7201 cttgatagaa aacaaattgg taatattcat atgatttcaa cccctaaata agtacatcta
     7261 atatcccacg aatagctgaa gctaaagttt tcgcagtcca gtcatttaaa cccgattctt
     7321 tcaatccaga tgcaataatt tgctctattt gatataaacc gccatctatc gtctcattta
     7381 ttctgcgaag aggaccttca atataggata aagcttctcc gaatctaaca gctgatttag
     7441 gtcctaatga tttaccaact atatttgtta atgtttttta tttctcaaaa tccatttcgc
     7501 tatataacga gcagatttag tcgcaccagc tctctcaaac ggagaatgaa tttcaagacc
     7561 ttcttctcta gctatttgtg ccaatttgaa gaattgttca aaggtcatat gcaacctacc
     7621 atcttcaaaa ttagcatcat cataaatgtt aattacagca ttttcatcat acgaccaatc
     7681 tgctttattt tggtctgcta gcactattac cgacggtgta attacattat acaactagaa
     7741 ccaataataa cgatgcaatt tttctcataa tctttttcat aaaatcatct ccagcgctat
     7801 atatttctaa ttagttatag attttgaatg catcatcgtc gtttttacca acgaatggca
     7861 ttgctttacg caattctgca ccaacttttt caatttcaag gttagctgct tgttcacggt
     7921 aagcagtcaa ttttggacgt ccagctttat agtcatttac aaagtcattt gcaaatttac
     7981 cattttggat gtctgccaag acagccttca tattttcttt aacttgttca gtgattacac
     8041 gtggacctga tacatagtca ccgtattcag cagtgtttga aatagattga cgcattttct
     8101 tgaatccacc ttcatagatc aagtcaacga tcaatttcat ttcgtgaaga acttcaaagt
     8161 aagccaattc tggagcgtaa cctgcttctg tcaagacttc gaaacctgct tcgataaggg
     8221 cagtcaaacc accacaaagt acagcttgtt caccaaacaa atcttcttca gtttcttctt
     8281 tgtaagttgt ttcaagaaga cctacacgag ccgctccaac acctttacac cagtccatag
     8341 caatgttttt agcatttcct gttgcatctt ggtatactgc ataaagagct ggaacaccaa
     8401 atccttcttc gtaagtacga cgtaccaagt gtcctggtcc tttaggagca cacatgaaga
     8461 catctacatc cgcaggaact ttgataaatt caaagtggat gttgaaacca tgggcaaatc
     8521 caactgcgtt tccagcttcc aagtttggag cgatttctgc ttcgtacaat tcttgttgaa
     8581 tttcgtctgg cgccaagatc atgataacat cagccaactt agtagcttct gctactgtgt
     8641 aagtatcaaa tccatcttct tttgctttat caaaagattt acctggacgt acacctataa
     8701 taacgtcacg acctgaatca cgcaagtttt gagcatgcgc atgcccttgt gaaccataac
     8761 cgataacggc gattttttta ccgtcaagtg ctgctacttt aacatctttt tcatattcca
     8821 tttgaactgt catagctttt ctctcttttc tattatttat tgccttttag gctggtttaa
     8881 taaatttaag ttggattttt aatcgcgggt aaatccagtt gcacccgttc gagcaatatt
     8941 gcgaataccg tatgggcgaa tgactctcaa tagggcttcg ctcttttctg catttcccgt
     9001 catctgaatg gtaatcgagc ttggcgctac gtctaccact gttgcacgga aaggttgaat
     9061 aatcgctaaa atctcagctc tcttctcagc tggcgctgac atcttaacca aaatcacctc
     9121 gcgctccaaa tgaggcttgt ctgtaatatc tcgaatgcga atcacatcaa tctgacgatt
     9181 gagctgtttg atgatttgct ccacttcatc atgagaagca acatcaataa taatagtgat
     9241 acgcgataca ttcggatctt ctgttgctcc aacagagatg ctttcaatat taacctgacg
     9301 acgagatagg acacctgtaa agcgattgag gactcctgaa cgattttgta gttttgctgt
     9361 taacattcta cgcatggaac tgcaccccca acatctcatg attactctta ccagccggta
     9421 ccattggtaa cacctgttcc ttacgagaaa tatctacctc aattagcata ggaacatcct
     9481 cagtgatgac ttcaaggtct tgagccaagg tctcaggatt gtcaaactta tagtttttaa
     9541 tgccataagc ctgcgccatc aattggaaat caggaagggt atcaaagacc gactctgatg
     9601 ttctgccttc atagaaggat tcctgccact ggcgaaccat tccgagtgaa tgattgttca
     9661 gcataaccac cttgattggc accttgtaaa tattcaaaat agccaactcc tggttagtca
     9721 tttggaaacc accatcccca acaaacaaga ctacttcctt atctgggtta gcaattttag
     9781 caccgattgc tgctggaatt ccaaagccca ttgttcccaa accacctgaa gtcactaact
     9841 gacgttcatt ttggtaggga taatactgag ctgtccacat ttggtgttga ccaacgtctg
     9901 ttaccacaat ggcatctcca ttcgtcaatt caccaattcg ttcaataact gcttgcggtt
     9961 gaaccacacg ctctttcttg tcataagaac gaacacgatt cttgtcttta gtgactttct
    10021 caatccactt ttcagtattg ttgtgaactg ttggttctgc tagcaacatt tgcaaggcct
    10081 tcttagcatc tccaactaca ggaatgtctg cactgataat cttgccaatc tcagctgggt
    10141 caatatcaat gtgggcaacc ttagcattct tagcgaaagt cttaggattc cccgtcaaac
    10201 ggtcatcgaa acgagaacca atactaatca taaagtccgc ttccgtcatg gcaatatttg
    10261 ctgcgaatga cccgtgcatg cctcccattc caagaaagag tgggtgactc gttgcaatcg
    10321 ttccttgtcc caaaagactg gttaccactg gaatttgata gcgttctgca aattcattta
    10381 gttccgtagc agcctcagca taactaattc caccaccagc taacaagact ggctttttag
    10441 ccttggacaa ttgcttcaag attttcttga tttgcatatc attcggctca agagtcggct
    10501 gataacttgg taaattcact tctggtgaat aaatgaagtc tgtttctaaa gcagatatgt
    10561 cttttggtag gtcaattaca actggccctg gacggcctgt agttgcgata tggacagctt
    10621 ccgtaatgat acgcggaata tcagctgtct cacgaacttg gtaattgtac ttagtgattg
    10681 gcatggtaat tcctacgatg tctgcctcct gaaaggcatc cttcccaatc cctgctcgcg
    10741 ccacctgacc tgtaaagacc aaaaggggaa cgctatcgct catagcatcc gcaatccctg
    10801 taatggcatt tgttgctcct ggtccactag tgacgacggc aacacccaac tttccagttg
    10861 atttggcata accttcagct tcatgcaaac aaccttgctc atggcgccct agaatgtggc
    10921 gaatgccttt aaaattatat atcgcatcat aaaaaggcaa aaccgcacca ccaggataac
    10981 caaagatggt atcaactcct aaatcacgaa gtgtttccaa aactaggtcc gaccccgtct
    11041 taggagattc taaactgatt ttctccattg ttcccctttc tttcctctta aaaataactt
    11101 gttactatca taccattttt tcaaa
//
LOCUS       DAVKGU010000060        11052 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKGU010000060 DAVKGU010000000
VERSION     DAVKGU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.060"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            12..347
                     /locus_tag="VW077_001480"
     CDS             12..347
                     /locus_tag="VW077_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455480.1"
                     /translation="MGGILLLICPFVLLGTAVNTAATTLNGGATAGAFSGVALLLNTL
                     KIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILAIIGGSLFLA
                     TLKKFKSEE"
     gene            883..1575
                     /locus_tag="VW077_001481"
     CDS             883..1575
                     /locus_tag="VW077_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9455481.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1628..4039
                     /locus_tag="VW077_001482"
     CDS             1628..4039
                     /locus_tag="VW077_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9455482.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASNGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRYCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4289..4990
                     /locus_tag="VW077_001483"
     CDS             4289..4990
                     /locus_tag="VW077_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455483.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            4992..7700
                     /locus_tag="VW077_001484"
     CDS             4992..7700
                     /locus_tag="VW077_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9455484.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEN
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7796..8287)
                     /gene="tpx"
                     /locus_tag="VW077_001485"
     CDS             complement(7796..8287)
                     /gene="tpx"
                     /locus_tag="VW077_001485"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9455485.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8410..9339)
                     /gene="psaA"
                     /locus_tag="VW077_001486"
     CDS             complement(8410..9339)
                     /gene="psaA"
                     /locus_tag="VW077_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9455486.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9371..10219)
                     /locus_tag="VW077_001487"
     CDS             complement(9371..10219)
                     /locus_tag="VW077_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9455487.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10216..10938)
                     /locus_tag="VW077_001488"
     CDS             complement(10216..10938)
                     /locus_tag="VW077_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9455488.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3235 a         2061 c         2521 g         3235 t
ORIGIN      
        1 tcgtcggtct tgtgggagga attttacttc ttatttgtcc ttttgtcttg ttgggaacag
       61 cggtaaatac agctgctaca actcttaatg gaggagctac tgcaggggct ttttcaggtg
      121 ttgccttact cttgaatacc ttgaagattg caaatcttgt tcttggtatc attgctattg
      181 tttactataa aggagataag catgtaggtg cagctccgtc tgtactaatg attgtttctg
      241 gtgaagttag tctcattcca ttcttaggat gggttggggg gattcttgct attatcggag
      301 gatctctatt ccttgcaaca ttgaagaaat tcaaatcaga agaataaaag gtattttagc
      361 atgaaaagaa caaaaaagtt tatcggtata ggagtagctc tattatctct ttctcttcta
      421 gttgcatgtg gaacataaag ttcaaagaat acttcaacaa gtaatgatga gaagacagta
      481 gcaacatcca atagttcaaa agaaacaatc actttcgata caccggttgt aacagacgat
      541 gcgattgaat caatacgcac ttatgcagat tatatagatc tttataaaaa tatttttgat
      601 gattatttta ctaaagctga ggaaggtttc aaaggcacag ctatggaaaa taatgactcg
      661 tttactaaac taaaagagtc aactcaaaaa ttattcgatg cgcagaaaaa aaggttaaat
      721 aaatgaagat agaatagaaa caaccaaaaa caatgtgatt gccaaacatt gtcaaacagt
      781 cctttccttt ttggttttga ctagcttttt tgtgaaaaat tgtgtaaaat agaatagata
      841 aacgagggga aacctcggaa aatttaaagg agaatccatc taatggtaaa attggttttt
      901 gctcgccacg gtgagtctga atggaacaaa gctaaccttt tcactggttg ggctgatgtt
      961 gatttgtctg aaaaaggtac acaacaagcg attgacgctg gtaaattgat caaagaagct
     1021 ggtatcgaat ttgaccaagc ttacacttca gtattgaaac gtgctatcaa aacaactaac
     1081 ttggctctcg aagcttctga ccaattgtgg gttccagttg aaaaatcatg gcgcttgaac
     1141 gaacgtcact acggtggttt gactggtaaa aacaaagctg aagctgctga acaatttggt
     1201 gatgagcaag ttcacatctg gcgtcgttca tacgatgtat tgcctccaaa catggaccgt
     1261 gatgatgagc actcagctca cacagaccgt cgttacgctt cacttgacga ctcagttatc
     1321 ccagatgctg aaaacttgaa agtgactttg gaacgtgctc ttccattctg ggaagataaa
     1381 atcgctccag ctcttaaaga tggtaaaaac gtattcgtag gagctcacgg taactcaatc
     1441 cgtgcccttg taaaacacat caaaggtttg tcagatgacg agatcatgga cgtggaaatc
     1501 cctaacttcc caccattggt attcgaattc gacgaaaaat tgaacgtcgt ttctgaatac
     1561 taccttggaa aataaaaaat tgtaagtcta gaattgattt ctaggctttt tatgttagta
     1621 tggaagtatg ataaggaata aaaaacaaga ttatgtactg gcctacaagc aaccagcttc
     1681 aaccacttac atgggttggg aggaagaagc tttaccgata ggcaatggtt ctttaggagc
     1741 aaaagtattt ggccttatag gggctgaacg gattcaattt aatgaaaaaa gtctctggtc
     1801 tggaggtcca cttcctgata gttcagatta tcagggtgga aatcttcagg atcagtatgt
     1861 ttttttagct gagattcggc aggctttgga gaagagagat tacaatctgg ctaaggaact
     1921 ggctgagcag cacctaattg ggccaaaaac gagtcaatat gggacctatc tgtcttttgg
     1981 ggatattcac attgagttca gccagcaagg tacgactttg tctcaggtga cggactatca
     2041 gagacagttg aatattagta aggcacttgc gacgacttct tatgtctata agggaacgcg
     2101 atttgaacgt gaagcttttg cgagttttcc agatgatctc ttggttcaat gttttactaa
     2161 ggaagggttg gaaactctag attttactat agaactatcc ttgacctgtg atttggcttc
     2221 taatggaaag tatgagcagg aaaaatctga ttacaaggag tgtaagttgg atattactga
     2281 ttctcatatc ttgatgaagg gaagagttaa ggataatgat ctgcggtttg ctagttatct
     2341 agcttgggaa acggatggag atattagagt ttggtcagat agggttcaga tatcaggagc
     2401 cagttatgcc aatctcttct tggccgctaa gacggatttt gcccaaaatc ctgctagcaa
     2461 ttatcgcaag aaactagatt tagagcaaca ggtgatagac ttggtggaca cagctaaaga
     2521 aaagggctat acccaattga aatcaaggca tatcgaggac taccaagcct tattccagcg
     2581 tgttcaattg gatttggaag ctgatgttga cgcatccact acagatgatt tgttaaaaaa
     2641 ttataagcca caagaagggc aggctttgga ggagctgttc ttccagtatg gacggtattt
     2701 attgattagt tcgtccagat actgcccaga tgctctacca gctaacctac agggagtctg
     2761 gaatgcggtc gacaatcctc cttggaattc ggactatcac ttaaatgtca atctgcagat
     2821 gaattattgg ccagcctatg ttaccaatct tctagagacg gtctttccag tcatcaatta
     2881 tgtagatgat ttgcgtgtct atggtcgtct agcggctgta aagtatgcag gaatcgtctc
     2941 tcagaaaggt gaggagaatg gttggttggt tcatactcaa gcgactccct ttggttggac
     3001 ggcacctggt tgggattact attggggttg gtcaccagct gccaacgcgt ggatgatgca
     3061 aaccgtttat gaagcctatt cattttatag ggaccaagac tatctcaggg agaaaattta
     3121 tcccatgttg agggaaacgg ttcgtttttg gaatgccttt ttacataagg atcagcaggc
     3181 gcagcgttgg gtgtcttctc cgtcttattc cccagaacat gggccgattt cgattggcaa
     3241 tacctatgac caatctctga tttggcagtt atttcatgat tttattcagg ctgctcagga
     3301 attgggactg gatgaggact tgttgactga ggttaaggag aagtctgatt tactaaatcc
     3361 tttgcaaatc actcaatctg gtcgaatcag ggagtggtat gaggaggaag agcagtattt
     3421 tcaaaatgag aaagtggagg cccagcatcg gcacgcttcc catctagtgg gactctatcc
     3481 tggcaatctc tttagctaca agggacaaga gtatattgaa gcggcgcgtg ctagcctcaa
     3541 tgatcgtgga gatggcggca caggctggtc caaggctaat aagatcaatc tctgggcgcg
     3601 tttgggagat ggtaatcgag cccataaatt attggcagag cagttaaaga catccacctt
     3661 gccaaatctt tggtgtagcc atcctccttt tcagatagat ggtaattttg gtgctactag
     3721 tggcatggca gaaatgttac tccagtctca tgcagcttat ctggtacctc tagctgcctt
     3781 acctgatgct tggtcaacag gttctgtttc aggcttaatg gcacgtggac attttgaagt
     3841 gagcatgagc tgggaagata aaaaactctt acagttgacc attttatcaa ggagtggagg
     3901 agatttgcga gtttcttatc cagatattga gaagagtgtg attaaaatga atcaagaaaa
     3961 aataaaagcg aaatgcatgg ggaaagattg tatttcggtg gcaacagcag aaggtgatct
     4021 tgttcaattt tatttttaag aagatgttat aaggcagtaa tttgaaactg ccttttaata
     4081 aggatttaag aatgtaagca gtttccaact agttgaaaaa gcgttataat gataatagga
     4141 agtaatactc aatgaaaatc aaagagcaca aactaggaag ctagccgcag gttgctcaaa
     4201 acagtgtttt gaggttgcag atggaagctg acgtggtttg aagagagatt ttcgaggagt
     4261 ataatttgtt tgatagaggg tgggtctgat ggcttatatt gagatgaaac actgttacaa
     4321 gcgttatcag gttggggaca cggagattgt ggccaatcgt gatgtgaatt ttgagattga
     4381 aaagggggag ctggttatta tccttggtgc ttcaggtgca ggtaagtcaa cagttcttaa
     4441 ccttcttggg ggaatggata ccaatgatga aggggaaatc tggattgatg gtgttaatat
     4501 tgcggattat agttcccacc agcgcaccaa ttaccgtaga aatgatgtgg ggtttgtttt
     4561 tcagttttat aatctagttt ctaatctgac agctaaggaa aatgtggaat tggcttctga
     4621 aattgtgaca gatgccttga atcctgatca ggtcttgaca gatgtaggtc tggctcatcg
     4681 tctcaataac tttccagccc agctttctgg aggggagcaa cagcgagtct ccattgcacg
     4741 cgcggtagcc aaaaatccta aaattcttct ttgtgatgaa cctactggag ccttggatta
     4801 tcagacgggc aagcaggttt tgaaaattct ccaagatatg tctcgtcaaa agggagcgac
     4861 ggtgatcatc gtgactcata atggagcttt ggcgcccatt gctgatcgcg tgattcatat
     4921 gcacgatgcc agtgtcaagg atgtggtgct caaccagcat cctcaggata ttgacagttt
     4981 ggagtactag tatgatcaag cgaaaaactt attggaagga cttaattcag tccttcacag
     5041 gctccaaggg gcgtttttta tccatcttga tcctgatgat gttgggatct ctagccttag
     5101 taggcctcaa agtaaccagt cccaacatgg aggcgacagc taatgcttat ttaacaactg
     5161 ctcaaacctt ggatttggca gtcatgtcta actatggctt ggatcaagca gaccaagaag
     5221 aactaaaaca gacggagggc gcagaggtcg agtttggcta tttgacagat gtgactatgg
     5281 ataatgggca ggatgccatt cggctgtact ccaaaccaga gcgaatttca acctttcagc
     5341 taagaaaggg acgacttcct cagtcagaca aggaaatcgc tttggccact catttgcaag
     5401 gccaatacag cgtgggacag gagattagtt ttaaagaaaa agaagagggt cattcctctt
     5461 taaaagacca tacttatacc attactggtt ttgtggattc ggctgaaatc ctctcccagc
     5521 gagatatggg ctacgcagga agtggaagtg ggactctgac agcctatggg gtgattttac
     5581 ctagtcagtt tgatcagaaa gtctacaata tagctcgttt gaaatatcaa gatttagcag
     5641 gtttaaatgc cttttcatca gcttatgaag aaaaatccaa gcaacatcag gaagagcttg
     5701 aacaaatttt atcagataat ggcaaggtac gtctgcaact tttgaaaaaa gaaggacaag
     5761 agtctctaga caaggggcaa gagacccttg acaaggctca gactaatttg caggaaggca
     5821 agcgtcgttt agcagctgct caagctcgta tacaggctca agaaagtcaa ctagccttgt
     5881 ttcctcaagt tcagagagag caggctagtg ctcaacttac ccaagccaag caggaattgg
     5941 gcaaggaaga ggccaaacta aagcaagctg aacaaaatct agcccaagaa aatgaaaaat
     6001 tagaaaaaca tcagcaagtc ttggacgatt tggcggagcc aaggtatcag gtctataatc
     6061 gtcagaccat gccaggtggt cagggctatc ttatgtatag caatgcttca tccagtattc
     6121 gagcagtggg caatatcttt cctgtggtac tttatgccgt agcagccatg gtgaccttta
     6181 cgaccatgac tcgctttgta gacgaagagc gaactcatgc agggattttt aaggccttgg
     6241 gttatcgtag taaggatatt atcgccaagt ttctccttta tggactagta gctgggactg
     6301 tcggaacggc tctaggtagt atacttggtc attatttgct agccagtgta atttcaagtg
     6361 tcattacaaa aggcatggtg gtgggagaaa ctcagattca gttctattgg acctatagct
     6421 tactagctct tgtcttgagc ttgttggcga gtgtgttacc agcctatctg gtggcttgga
     6481 gggaacttca tgacgaagca gcccagcttc tacttcctaa acctcctgtc aaaggagcta
     6541 aaatcttatt ggagcgtatc ggttttatct ggcgtcgtct cagttttact cataaggtaa
     6601 cagcccgcaa catctttcgt tataagcaga gaatgttgat gacaatcttt ggtgtggcag
     6661 gttctgtagc tctgctcttt gcaggtttgg gaatccaatc ttctgtagca ggagttccgt
     6721 ctaaacagtt tcaacaaatc caacagtatc agatgcttgt ctctgaaaat cctagtgcga
     6781 ccaatcagga caaggtagag ctagcagaag tgttgaaagg gcaggagata ctagcctacc
     6841 agaaaatcta ttctaaaacg ctagacaagg atttcaaagg caaggctggt cttcaaaaca
     6901 ttactcttat gatgatagag aaggaagatt tgactccctt tatccatctt caacatcatc
     6961 agcaggagct gacattaaaa gatggcatcg ttattacagc taaactcgcc cagctggcag
     7021 gtgtcaaggt tgggcagact ttagaaattg aaggtaagga actaaaggtc gctgctatta
     7081 ctgagaacta cgttggtcac tttatttata tgagtcaggc tagctatgag caactttacg
     7141 gacagctacc ccaagccaac acttatctgg tctcattaag ggataccagt gcaactagta
     7201 tcgaaagtca ggcgggcttg cttatgaatc aatctgcggt gtccagcgtt gtccaaaatg
     7261 cttcagccat tcgactcttc gactctatcg ctagctcact caatcagacc atgaccatct
     7321 tggtcatcgt atctgttcta ttagctattg tcatccttta caatctgacc aatatcaacg
     7381 tagctgagag aatccgtgaa ctctccacta tcaaggttct cggttttcat aataatgaag
     7441 tcacactcta catttaccgt gagacgattg tgctgtccct tgtgggaatc gtacttggtc
     7501 tgatagctgg tttctattta caccaatttt tgattcaaat gatttcgcct gcgactattc
     7561 tcttttatcc gcaggtaggc tgggaagtct atgtaatccc agtggcagca gtaagcatca
     7621 ttttgacctt gcttggtttc ttcgtcaatt attatctgag aaaggttgat atgttagaag
     7681 ctctgaaatc tgtagagtaa ggtagttatt tttagctgat tgaacttcta tttactaata
     7741 ttcaaaaatc ctccgtttca aagagcaggg aactctttgt gacagaggat tttttctata
     7801 gggctttagc agctgcaatt gcggcttcga agtttggctc agaattgata ttatccacgt
     7861 attcaacgta gcgaatcgta ttgtcagtat cgaggacaaa gactgcgcgt gctaataggt
     7921 gccattcgtt gattaagagg gcataatcgt gcccgaaaga atggtcaaag tagtctgaaa
     7981 gcataatggc attgtcaagg ccttcagcac cgcaccaacg tttttgagca aaaggtaggt
     8041 ccattgaaac agtcaatacg accgtgttgt ccagtccagc caattcttca ttaaaacgac
     8101 gtgtttgagt tgagcagatg cctgtatcga tagaaggaac gacactcaag acttttttct
     8161 tgccatcaaa atcagccaga gattttttag aaagatctgt tgtagtaaga gaaaaatcaa
     8221 gcgccttgtc gccaacttgt agttgtttac ctgtaaagct cacaggattt ccgagaaaag
     8281 ttaccatagg atactccaat cttttttctt ccattgtatc tgaaacagtc agaattttcc
     8341 aatgatttga ccggaaatgt gggcatagaa aaaacgccag ctcacatgag aatgacgttt
     8401 ttcagaggct tattttgcca atccttcagc aatcttgtca aggttgtatt tcatcatgct
     8461 gtagtagctg tcgccttctt taccttgttc tgcgatagag tcagtaaaga tttgtgcgta
     8521 gattgggatg tttgtgtctt gagaaacagt tttcattgga cggtcatcca cacttgattc
     8581 tacaaagagt gatggaactt ttgtttggcg aagtttttca accaaggtct tgatttgttc
     8641 aggagttcct tcttcttcag tattgatttc ccagatgtag gcacttggga caccataggc
     8701 tttagagaag tatttgaatg ctccttcgct ggttacaatg agtttctttt cagcagggat
     8761 cttattaaat ttatccttac tttctttatc aagtttgtct aacttatcag tatattcttt
     8821 gagatttttt tcatagaatt ctttattgtt agggtctttg gcgctcaatt gtttggcgat
     8881 atttttagca aaaataatac cgttttcaag gttaagccaa gcgtgtgggt cttcttttcc
     8941 tttttcattt tgaccttcaa ggtagataac atcaacgccg tcgctgactg cgaagtagtc
     9001 tttgttttca gttttcttgg cattttctac caattttgta aaccaagcat tgccacctgt
     9061 ttcaaggttg ataccgttat agaaaatcaa atcagcctca gaagttttct taacgtcttc
     9121 aggaagtggt tcgtattcgt gtgggtcttg cccaatcgga acgatactat gaaggtcaat
     9181 tttgtcacca gcaatatttt tagtaatatc agcgatgatt gagtttgtag caacaacttt
     9241 tagtttttga ccagaagttg tatctttttt tccgctagca catgctacaa gaatgattgc
     9301 agaaagaaag agaacgagta atgtacctaa ttttttcatt agatcctcca atttattagg
     9361 gctttgcccc ttattttaac aaatgtttat ttttcagttt caaatatcgt tgtttgggag
     9421 cgataaagaa gctaatgaga aagaaactag cagctgtaag cacgatacta gaacctgccg
     9481 caacattaaa actatagcca ataaagagtc ccaaaactga agcagtagct ccgaaggttg
     9541 aggaaaggaa aatcatactt ttcagactat tagcatacag ataagcagtt gcagctgggg
     9601 taatcagcat ggctacaatc aggatagttc cgacactttg catggctgtc acagacacga
     9661 gagtcaggag taccatgaga aggtagtgat agaaattgac aggcattccc atggctttag
     9721 ccaagagttc atcaaaggaa gttatcaaga gttgcttgaa gaaaatccaa attaacaaga
     9781 ggatggctgc ccccacaccc atagtaataa acatatccgt atcttggacg gccaggatat
     9841 taccaaaaag gatatggaaa aggtcagttg aacttttagc gacaccaatc aagatgatac
     9901 cgagggctaa gaaagaagaa aaggtaatgc cgatggcggt atcgcttttg ataatcgagt
     9961 ttcctttgat gtaggtaatg atgatggcag ctagcaatcc aaagacaatg gctccgataa
    10021 agaagtcaag gcccaagatg aaggagaggg ctacacctgg taagacagca tgtgaaatgg
    10081 catctcccat gagtgacatc ccgcgtagaa taatgaaaca tcccacggct ccagctacaa
    10141 tcccgacgac aatagctgtt atcaaggcat tttgtaggaa atggaatttt tgcaatccat
    10201 cgataaattc tgcaatcata ggtcacctcc attgaaaaag agttgattac cgtaagcttc
    10261 ttttagattg gtttcggtaa aagtttcttt tgttggacca aaggcaatca cttctcgatt
    10321 gacaagtaag acttgatcga agtagtgggg aatcttgctg aggtcgtggt gaacgatgag
    10381 aaccgtcttc ccagcttttt tcaaatctct cagcgtattc atgatgattt cctcactgac
    10441 agagtcaatc ccagcaaagg gttcatccaa gaggatatag tcggcttcct gcaccaaaca
    10501 tctggcaatc aagacccgct ggaattgacc tccagacagt tgactaattt gacgttcagc
    10561 gtagtcagct aggccgacga tttcaagggc ctcttgcact ttcttccaat gtttagcctt
    10621 taaacttcga aagagaggaa tagagggaaa tagtcctaac gagacgcatt ccttgacctt
    10681 gatgggaaag ttgtagtcga tattgatttt ttgttcgaca taggcaattc ggtgtaagga
    10741 ttttttaact tccttgtcat cgagaaatgc ctgaccttga tgtgggataa ttcccaacat
    10801 accttttaat agtgttgatt tcccagcgcc gtttggacca atgatgccgg taattgttgg
    10861 tccatggagc actagtgaaa tatccttaag tgccaacgtt tctttgtagg agacactgag
    10921 gttttcgata cgtatcataa acttgtattc ctcctgtctc ttaatataca ttaaaaaaaa
    10981 aattaagtca agttaatttt tgaaaaaatt aaaataataa ctgaaaaata gattctaaaa
    11041 ataactttca gg
//
LOCUS       DAVKGU010000061        10980 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKGU010000061 DAVKGU010000000
VERSION     DAVKGU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.061"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            95..259
                     /locus_tag="VW077_001489"
     CDS             95..259
                     /locus_tag="VW077_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9455489.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            343..909
                     /gene="amaP"
                     /locus_tag="VW077_001490"
     CDS             343..909
                     /gene="amaP"
                     /locus_tag="VW077_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9455490.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            921..1091
                     /locus_tag="VW077_001491"
     CDS             921..1091
                     /locus_tag="VW077_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9455491.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1130..1738
                     /locus_tag="VW077_001492"
     CDS             1130..1738
                     /locus_tag="VW077_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9455492.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1769..1972
                     /locus_tag="VW077_001493"
     CDS             1769..1972
                     /locus_tag="VW077_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9455493.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2083..2355)
                     /locus_tag="VW077_001494"
                     /pseudo
     CDS             complement(2083..2355)
                     /locus_tag="VW077_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2664..2951
                     /locus_tag="VW077_001495"
     CDS             2664..2951
                     /locus_tag="VW077_001495"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9455494.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3229..3999)
                     /locus_tag="VW077_001496"
     CDS             complement(3229..3999)
                     /locus_tag="VW077_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9455495.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(4014..4862)
                     /locus_tag="VW077_001497"
     CDS             complement(4014..4862)
                     /locus_tag="VW077_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU9455496.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGTPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4859..6334)
                     /locus_tag="VW077_001498"
     CDS             complement(4859..6334)
                     /locus_tag="VW077_001498"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU9455497.1"
                     /translation="MVQTKQPNIILIVVDQMRADVLSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6657..8012)
                     /locus_tag="VW077_001499"
     CDS             complement(6657..8012)
                     /locus_tag="VW077_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9455498.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(8022..8339)
                     /locus_tag="VW077_001500"
     CDS             complement(8022..8339)
                     /locus_tag="VW077_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9455499.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8356..8673)
                     /locus_tag="VW077_001501"
     CDS             complement(8356..8673)
                     /locus_tag="VW077_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9455500.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8688..9584)
                     /locus_tag="VW077_001502"
     CDS             complement(8688..9584)
                     /locus_tag="VW077_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9455501.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9581..10849)
                     /locus_tag="VW077_001503"
     CDS             complement(9581..10849)
                     /locus_tag="VW077_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9455502.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLEDCE"
BASE COUNT         3778 a         2204 c         1701 g         3297 t
ORIGIN      
        1 aaaataaaaa aggagggtag gcagtgttgt gatcaattat tgctggaggt cttattggtc
       61 tcttggcagg taaaatcact aaaaaagtag ttctatggga atcatcgcaa atgtattcgc
      121 tggtttagtc ggggcatatg caggacaatc tcttttaggt agttggggtc catcaatcgc
      181 tggaatggct ttgctcccat ctattgtagg tgcagcgatt gtgattactg tagtgtcatt
      241 ctttacaggt agaaagtaaa cttttcgcca gtaaagttag caaactattt ttaaatcaat
      301 gacgggaaaa atagtttaaa tgttaaatcg aaaggattgt atatgtcaaa agcaaagaaa
      361 atatgtttca ttattttctg tattttaatc ttgacaattt tccttcctgt tttgatagat
      421 tatcatcaag ttagtgatct aggtattcat ctacttagct ggagacagaa ctccgtagtt
      481 gaattctatc ttgctagata tgtcttttgg gggacagtgg ttctatcaac tttagtttta
      541 ttatccattt tagttgtgat gttttatcct aaacgttact tggaaatcca acttgaaact
      601 aaaaacgata cattaaaatt aaagaattcg gcaatcgaag gttttgttag aagtttggtg
      661 agtgatcata gattgatcaa gaacccaact gttcatgtaa atttacgaaa aaataaatgt
      721 ttcgttcatg tagaaggtaa aattcttcct tcagacaaca tcgctgacag atgccaaata
      781 attcaaaatg aaataactaa tggattgaag cagttttttg gtattgagcg tcaagtaaaa
      841 cttgaagttg cagtaaaaaa ttaccaacca aaacctcaaa acaaaaagac tgttagtcgt
      901 gtgaagtaag gaagtaaaaa atggaatggc ttaaacaata tcgatatcca attatcgctg
      961 gtctcatagg cgtatttctg gcttgtttga ttgtctcctt tggcttcttc aaaacaatat
     1021 ttgtattgat tttaggagca ctgggagttg cagctggatt atatatcgaa aaaaactata
     1081 tagataaata aaaaaataaa aattactaat ttaattaaag gagtttcata tgtcaaacga
     1141 aaaaaacaca aacactaacg tagaaaagaa agatgctact gttgtagctc acgaaatcaa
     1201 aggggaactt acttacgaag ataaagttat ccaaaaaatc attggtcttt cactagaaaa
     1261 cgtttcaggt cttttgggaa tcgatggtgg tttcttctca aatcttaaag aaaaaatcgt
     1321 taacagcgat gacgtaacaa gtggtgttaa cgtagaagtt ggtaaaacac aagttgcagt
     1381 tgacttaaac gttattgttg agtaccaaaa aaatgttcca gctttatatt cagaaatcag
     1441 agaaatcgta tcttcagaag ttgctaaaat gactgacttg gaaattgttg aaatcaacgt
     1501 aaacgttgtc gacatcaaaa ctaaagaaca gcatgaagca gactcagtaa gccttcaaga
     1561 tcgcgtatct gacgttgctg aatcaacagg agaattcact tcagaacaat tcgaaaaagc
     1621 taaatctggt cttggatctg gtttctcaac tgttcaagaa aaagttagcg aaggtgtaga
     1681 agctgttaaa ggtgcagcaa atggtgtagt atctcacgaa aacactcgtg taaactaaga
     1741 taaaataaat ataacaggag aaattatcat gtcagtagaa gaaaaattaa atcaagctaa
     1801 aggttctatt aaagaaggtg ttgggaaagc catcggtgat gaaaaaatgg aaaaagaagg
     1861 agcagctgaa aaagttgttt ctaaagtaaa agaagttgcc gaagacgcta aagacgctgt
     1921 agaaggtgct gtagaaggtg ttaaaaacat gttgagtggc gacgataaat aaggttaaaa
     1981 gttactttat cttttttggt aacattagtc aaaagagtct gagtcaagat gattctcaga
     2041 aaacaaaaag ctagagattc ccaattgcgg aactctagct ttttaatttt gcctctttct
     2101 cttattatat ttcagcaggt tgttggccat gagtatgaat cccatgtcaa ttctcacttg
     2161 acgcttacct ctcagatgac atctcttata acccaaacaa acctttatct gcccaaagac
     2221 agatttcata tcaatcttac gtttagcgaa aatttgtcta cccttggaag ataaaagtgc
     2281 ctgatattct ttagttttta aacactggta acgttcattc atatacagtc ccttttgagg
     2341 ggctgattca ggttcataat cgcagtcaac attgatttca aggctgtttg ctttctatct
     2401 cccctttttc ataatgtata gtaaaatgaa ataataacag gacgaatcga tcgggacagt
     2461 caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattctagtt tcaatctact
     2521 atattttcgt acaggtgctt caatagtttc tatagtaaaa tgaaccaaaa atagtacacg
     2581 ttgcggtata atgttcttgt ggcatattca ttagattttc gtaaaaagtt ctctcctatt
     2641 tgtgagcgaa caggtatagt caattgattg tcagtttgaa gaccaagagt cgtaaggcaa
     2701 aggatatggc agaatccatc caagggtggc tggcacaatt tctagtgaac ctctttaaat
     2761 ctattacgtt tgactgtgga aaagagtttt caaaatggaa agatatttcc aatcatcacg
     2821 atagtgagag cttttttgcg aatctaggat gtccaagaca gcggtgtctc aacgaacatt
     2881 caaatagact tttaaggtgt catgacttgc caaagcagac agatttcaac gaagtcagtc
     2941 aagaatttta ggctgggtaa aaactcaatt tcaggagaaa atgaaataaa tattcccaca
     3001 ataaaacgca tagtatcaaa gtttttccaa cacctgatat tatacatttt ttaattttaa
     3061 agactttttc ccagcctcaa gaaatcacat cccacctaaa tcctttggat accaaatacc
     3121 tcatcaggta ttttctgagt tggtaagact gtctagctta atttgacaaa tggagggcgt
     3181 taaatccagt ttttacagga agtctattta aatattttca cactgacctt acaaaatatg
     3241 aaataaatta tataaagtca ttcccatgac aaagaataac atccctttaa aacagtatct
     3301 tacccaattt tcaggtaaga cttttgatac aaccgtcccc aatacccctc cgcatataga
     3361 agcaagaaaa ataactggaa caggtgctaa atgatactga tttaaacctg taaccacaac
     3421 aattgtagcc aaatgagaca actgggaaaa gaaaataatt gcaagagaat acatcgttgc
     3481 ttcttttatc gaaatagaaa aaaacaccat caataaagat acattgagcg gaccaccacc
     3541 aatccctaaa aaactagaaa tcaagcccaa tagaagacca caaatcatat agaatcctat
     3601 cttatcaaat tgataagttt ttttaaaatc tgttaaaaca aatgatacaa gtagcatcac
     3661 aaatagtaaa atcatttgaa caatggatac tagccgtact gataactgag ataacaaaac
     3721 ttgaaaaata agatgaccta gaaatcctcc taaaacagaa gaaaaggata ccgtaaaaat
     3781 caatctccat tttatctctt tactttgtga aaatcttttg attgttgaaa taatcgccat
     3841 tataaaaaca gagaaactag aataaaaagc aatatcactg accgaatggt atccaaaact
     3901 atccataagt ggcttaatta taattccacc acccatgcct gatatagaac ccaatccact
     3961 agctattata atgattagaa aataaataat ctcaaccact ttttaagtcc atcctattct
     4021 ttcaaacatc taaaacctag atggctacta gtggaagtag aaatacatcc atttctagca
     4081 gctactcggt atcgattaca ataagaacaa tgacataaaa aggaacctcc tctgatagca
     4141 tactcctcat cttgtatccc atattttggt aactcatagt catcttcatt aaaactagct
     4201 aataaggtat atctcggatt gcgacaccat tcccaaacat tcccaatcat ttgatataga
     4261 ccaaaatcat taggtaaaaa ttcatagaca ggagccgttc ctataaatcc atctaaggca
     4321 gtattttcat aaggaaatct tccttgccaa gtattggcat gataatatcc accttctaaa
     4381 agagtatctc cccaaggata ttcagattgt cttcctcctc tagcagcata ttcccactgt
     4441 gcttcagtcg gtaaagacat tccgctccaa ttacagaaag ccaatgcatc ttctaaagca
     4501 acatgaacta ctggatggtc ttctaaaccc actagattac tattctcacc gtagggatgt
     4561 ttccagcaag cgttcggaac ttgtaaccac cacggagtcc cagcaggatg aggatatccc
     4621 tccctttctg cctctggaac gaacaatata aaaacaaaag accactcttg tcgctcagct
     4681 agtgttttat agcctgtttc tttcacaaat tgagcaaatt cttgattcgt aactggagta
     4741 tccgcaatgg aaaaatcctt tacactaaca gcgacttgag gaccttcaaa atcatctaaa
     4801 aagccctctt cagaatttgt tcccatcata aatgtcccac ccttgatttt tttcattttc
     4861 atgattgact atcccccgtt ttcgttagag ttggcgttat tttagaaagt tcaacaggaa
     4921 ctaactcttc atccacaaca aaaccttctt ctctatatct aagaaagtct actaattttt
     4981 tcttcatctg acgaacaata ggttggtatt tttcacttgg atacaaatca ttcatttcat
     5041 gcggatcttt tttcatatca aataactgat aatgatttaa aacaggaaac cagataaatt
     5101 tccactggtc tgtcaagata tattgactag aatcttttcc taaagcatgt tcaccatgaa
     5161 attctgttcg ccacccctcg tactgtccaa ataataaatt cttaacactc cgtccatcta
     5221 gttcatctgt agtagttcct cctgccaaat ccacgagtga aggaaaaata tcttgaattt
     5281 tcactaattg cttgattgtt cccctattac cagctatcaa tccagccgga tcgtaaataa
     5341 acgaaggaat gtggatactt ccctgataag gatatccctt tctaaacaga taatgttcac
     5401 caagctgatc tccatgatcc gaaacaaacc agataatcgt atctttatca tgacgaaatt
     5461 cttttagggc agtcaaaaag cgactaattt gatggtctat atgagtaatc aatccaaaat
     5521 aagcagcaac cattcttctt tgatcatctt cctttaattt ccctcttaaa gcataaatag
     5581 acggaatttg cttttctaat acttcccaat tcccgatatg caaatcgaga aattgaggca
     5641 agcgttccat atacatatca aaatagtatt ttgggggatt taatggagca tgtggttttt
     5701 caaaagacat tttgagaaaa aaaggaaccg ttggatctct tctttgtaaa aatgaaatgg
     5761 actcactcac aacccagttt gttggatgta acttctcatc tttatcccaa ggtctagctt
     5821 cccaagaatt acaatccatt ccgtcatcaa tcaaatcaac atcataacca actttaccct
     5881 ttaaaaaggc tagatagtca ctagcataat caaattgact tccataagcc ttatcatact
     5941 ttctatcaac atgtagatac ccatcgtgaa gtaagacatg atcaaaccca agtctctgtc
     6001 tggaaggaaa aacatgcatc ttaccaatac actctgtttg ataacccata tctttaaaga
     6061 cttttggaag agtattagta aaattccaag gcacctcatc ttgataacca actcgtccac
     6121 ttttatcttg gtctaagcca gtcaataaag ctgccctagc aggaacacaa gaaggaactg
     6181 gagaataggc attttcaaaa ttataaccta cactagccat catatctaat gttggagtgc
     6241 taactagctt atccttacta ttcaaggata agacgtctgc tctcatttga tctacaacaa
     6301 tcaaaataat atttggctgt tttgtctgaa ccataaaatc tcctttctaa tatggcaaaa
     6361 gaggcacaag aagatatcta cctttactgc acccctttct atatcaatct ctctatataa
     6421 aacaataaca ttcttgttat gttttataga aaaatgaact aaaatatgac taaatcgatt
     6481 aggaaattca aatcattttc tagtactgtt ttagaagtta cagtgtacta ttccaacttc
     6541 aataaattat aaacctttgt ctaataacaa ttttagtgga gataagaaat cctacaccta
     6601 actcatctta cacgtaatct atttctattt tatcacaaaa aacgcaagta agaccattaa
     6661 ctcaattcag ttttatctgc cattttcaca aatgggaaat aagtcaagac actaataatc
     6721 aaacaaacaa ctgataagat gatggcacgc caatcaaatg ctgtagagaa gaaaccatat
     6781 aaaattggag gcattaccca agtaacgttt tgtgtaacag gtgaaacaag accccagctt
     6841 gttgcccagt aagctaccgt tgccatgaaa accgggctaa gtacaaatgg tataaatagc
     6901 aaaggattca agacaactgg taaaccataa ttgaccggct caccaatatt aaacagaact
     6961 ggtgctagac caagtttagc aacttttcga taatgactgt ttcttgaaaa aattaaaata
     7021 gcaagtacta atcctaatcc tccaaaccag acaaacgccc caaaagaccc acttgtccat
     7081 atataaggaa tcggttcacc tttttggaaa gcatccagat tcgctaacat agcaactcca
     7141 aacagccctt ccatgatggg agccaataca tttcctccat ggagaccaaa aaaccagaat
     7201 aacttattca aaaagatcat cagaataact gcaaagaaac tttgagacaa acctagtaat
     7261 ggcgtttgta acaccttgta aacccaatca atcaataagt cattgctaag taaatggaaa
     7321 acataagtca agatggctac tatatacatc gccataaatc ctggaatgat agaagtgaac
     7381 ggcttagcaa tcgcaggggg aactgaatct ggtaacttga ttacccagtt ctttttcatt
     7441 actttacaga aaataataga ggctaaaaat ccaatcatca tggctgtaaa gtagcctctg
     7501 gcattaatat ggtttcctgg aatcacattc ccaatagtta ccatcagatt tttaccatca
     7561 aatgctagat tatcaattcc ttgtaagatt tgatctaatt tcacatctcc tacatttgcc
     7621 aaagggaaac tctttgtaac tgtacttcca atcgaaatga caaacgaagc aagcgatacc
     7681 aaaccagcag aaactgtatc aaccttataa atcttagcga tattcactcc caagcaatag
     7741 atgaacaata aggaaacaat tggtatactt cccttgaata ccaaattatt gatgtcaaca
     7801 agccactgaa aggttttcgt aatactttct aggtgaaatt gttgtggtaa atccactaga
     7861 aaagcattta ataacaaagc aatggaacct gtcataataa caggcatagt ccccacaaat
     7921 gaatcacgta aagcaactaa aaacttctga ttcccaactt tcattgcaat tggagaaagt
     7981 ttttcttgca ttttatccaa cattgtattc atagcaaatt cctaattttt ttcaaatcgt
     8041 tcgtatactt ctaccacttc tttcgccaag tcaacaaagg tcagactcgt cattaaatga
     8101 tcttgcgcgt gtaccatcaa gaggctaatt gatgtttctt ccccctgtgc ctcttttgtc
     8161 aacatttctg tttgagcctt atgcgcctgt aataaggcag cattcgcatc agctaatctt
     8221 cgattggctt tagagaaatc cccttttttt gctgcctgaa tagcctccat agcattactt
     8281 ttggcttccc caccatacat aattagcccc attgcagatt ctaaattact ttcattcata
     8341 gcattctcct caatcttagt ctaataaact ttcagctaaa tctaagactt tagaaccatt
     8401 catcattcca taatctgtca tcggaataac cgccacagga atctgtctat cttttagttt
     8461 ttcttgaaag tcccctagta aatagcgaac ttgagggcct aaaagcaaca catttacttc
     8521 ttttgttgct acaatttctt ctgcttcagg agctggaact gcaaaaattt ctgcatccaa
     8581 ccccttatct tctgctgcct tttgcatctt tgtcactagc agacttgtac tcatacctgc
     8641 tgcacatgct aacataattg ttactttagc catagcttac tcctttatca cctatttact
     8701 gacctagcca agcgagatac tgtatgtctc actcctctaa ttgccttgct caatgcataa
     8761 atattatcga ctgttgccag tcccccatat cctgcgtcac ctatatgttg aatatcaact
     8821 ccacaaattt tatttctaag tgcaatttcc ttaatagtat ctgtatcaga tgtttcttgg
     8881 ctagtaccaa tagcactcaa tacaagacca cccttactat gaacgagatc aacgacttca
     8941 cgcaactctt ggtcatgaaa ggctggaacg gttccaactg ctggaacaag tatcacatcc
     9001 gcacctgctt ccaacaattg ctctgcgaca gaaagctctg ccacaggctc attcactcct
     9061 gccccgtgca tcttacctgc aataatcaaa ccagaaaagt tttccttagc agtttgaaca
     9121 gccttaatga tttctcgatt gctaactcct gttccaggat ttccagtcaa acagacaaag
     9181 tcaaaaccta actcctctat tcgcttcaat gtttcaacac tagcaacacg acctgcaaca
     9241 atttcctgtg tttcctctag catctttgca gatgggtcaa tcggttccaa atttacacca
     9301 attggacaag caacaagctg gtgtaagcga tgaataacat catcacctga actatccaaa
     9361 gcataaattt taggctcaaa aacatcaaca caattcaata aaatcatatc agctccaaaa
     9421 gctctagcaa tttcagaatt agttatatcc ccaacaaatg tctcacgagt aactacattt
     9481 tcagatagaa caactctacc ttcacttgct aaaatacttt gttttaattc ttcagcattc
     9541 atctgtaata tctcagatgg atttgcacta attaaacgtt tcattcgcaa tcctccaata
     9601 acttaaaccg tttttcaata gctattacaa atggtaaata aagtaagaca tccaaaacaa
     9661 tcaatacaat ttgtaataat gaaccagaaa tacttcctgt agcaagtaaa ccacttataa
     9721 caggtggcat agtccaacct ggatctgtat aagttagcgg aaccaaacct gatgccattg
     9781 ctccccacgc tactagtaag ttaaacattg gagctaagat aaatggaact agtaacaaaa
     9841 tgttcaaaac aataggaacg ccaaacattg ctggttcatt tatattaaat aatccaggta
     9901 taacagttat aggtgccaaa gtttttagtt gtttactagc tttcttctta tgtgctaaat
     9961 atcctaacgc aagaactaaa cctatggttg caccaccgcc acctatgaat acaaaattat
    10021 ccataaatac gctagtaaaa atatttggaa gagtttctcc agtttgataa gctactttat
    10081 ttgcatctag attagctaac cataacggtt tcataactgc attaacaaca tttcctccgt
    10141 gtaatcctac aaaccaaaaa ctactattaa ggaaaattaa gataagaagg ccaataacat
    10201 tatttcctaa aagtccaagt ggacctccca aagctacttg agcaatttca tgaagattag
    10261 gtaaacttaa tttatctaaa gttgcataaa caatcaacca accaacaata gtcactgcac
    10321 caggaataat ggcactaaag cttttagata ctgctggtgg aacaccatcc ggcattttta
    10381 tctgtatatt atgatttata aaccattggt agatatagcc attaatcaat cctaaaatca
    10441 tagcaacaaa taaacctttt gatgccatga aagcggtagg cattcctgct ccagcctctc
    10501 ctctaatgaa aggcgtaact gtaataaaag atgataatga cacaatacca gcaggaatac
    10561 catcaacttt gtattgtcta gccataaagt aagcgatacc aaaactagct acaagaccaa
    10621 gtaatccaaa actactatca acacccttcc ataaatagtc agcaacacca atatcaccca
    10681 gatatttttc ccatcctgga ataggaaaac ttgcaataac cattagtaat gaacctatca
    10741 acaataaagg tatagttaga gtaataccgt ctcgaataga aaccaacgct ttattattac
    10801 ccaatctagc cgctactgga agtaactttt cttgtaataa tttttccata accttttcct
    10861 cttaaatata ttttattatc aaaagttatc aattatcttt attttttgac gtcaagttgt
    10921 tatttgatta tcttttgtta tttatatttt acaactataa gaaagccttg tcaaggattt
//
LOCUS       DAVKGU010000062        10922 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKGU010000062 DAVKGU010000000
VERSION     DAVKGU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.062"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(196..1827)
                     /locus_tag="VW077_001504"
     CDS             complement(196..1827)
                     /locus_tag="VW077_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9455503.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIGLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2116..7056
                     /locus_tag="VW077_001505"
     CDS             2116..7056
                     /locus_tag="VW077_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9455504.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRVE
                     YDFTDAYNGGNSLKFSGDVAGKTVQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTSDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFSDVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGKDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLHYQGH
                     EVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNT
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7146..8342
                     /locus_tag="VW077_001506"
     CDS             7146..8342
                     /locus_tag="VW077_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9455505.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINVLEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8478..9005
                     /locus_tag="VW077_001507"
     CDS             8478..9005
                     /locus_tag="VW077_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9455506.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9082..9438
                     /gene="glnR"
                     /locus_tag="VW077_001508"
     CDS             9082..9438
                     /gene="glnR"
                     /locus_tag="VW077_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9455507.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9475..10821
                     /gene="glnA"
                     /locus_tag="VW077_001509"
     CDS             9475..10821
                     /gene="glnA"
                     /locus_tag="VW077_001509"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9455508.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
BASE COUNT         3471 a         2012 c         2411 g         3028 t
ORIGIN      
        1 taaaatgaaa taataacagg acaaatcgat caggacagtc aaatcgattt ctaagaatgt
       61 tttagaaata gaggtgtact attctagttt caatatacta tatatctgca gaatactctt
      121 tcagtttcat tcagcctagt cttgcttagg aaaaagtaag aagccatcca attatgatgg
      181 atagcttctt ggttcttaga tttgttctgc aaaaaccttt tcagcaaggt tcatagcgtg
      241 gtctgataca cgagtgtagt gtgagatgat gtcgataaag ttgaccccag cttgtgttga
      301 acattcgcct ttgttgaggc gtttgatgtg ggtttttctg agaacacgtt ctatcttatt
      361 gattgcttca tgacgttcta caagactgcg tgctttttca atatcattgt tttccacact
      421 atccagagca tctttgataa agtcactagt ttggcggtaa acttcctcta attctttcaa
      481 ggcggcatca gaaaattcaa catttttccg ttgaagatag tcagtcagat tgagtagagc
      541 ctccgtgtgg tctccaatcc gttccaaatc acgggaggaa tcaaggatat tggtaagcac
      601 ttcactttct ttttggctga gagcttcgct tgaaagggca atgagataac gtgttaattg
      661 ctcatcgatg gtgttaattg cttcttcggt tttatgccct ttttcagcaa ctttttcatc
      721 caagccaatg atgtacttat aggaaaggtc aaaggcttta gcagcgtagt ttcctaagtg
      781 caagagctct ttcttagcat ttcctagagc gatagatggg gcttgtttga tgaaatgttc
      841 atcaagatat aagggttcgt atttgacaac ctcgtcctct ccaggaataa tcttggttac
      901 aaagtaagcc agagctccga taaatggaaa ttggacaatg gtgttggtaa tattaaaggt
      961 tccgtgagca aaggcgatgg tcatttccgg tgctagattt agcgtagctt caaaccaatg
     1021 aatcaggaca gtaaaaggaa ctagaaaaat aacgcagacg actgttccga taacgttgaa
     1081 ggcaacatga gctcctgcta cccgtttagc tgcaatatta gcccctaaag aggcaatgat
     1141 ggctgtaatg gttgtcccga tattgtcacc aaatagaact ggcaaagctc cctgtaggtc
     1201 aattagattg ccggcgtaga ggttttgtaa aatcccaatg gtagccgaag aagcttgaat
     1261 tagcaaggtc aagccagtac cgacaaagac acccaaaaca ggattcttac ttagctcaat
     1321 catatagtcc ttaaagacct gtaaatcctt gagtggagcc attgcgccgc tcatgagatt
     1381 gagggcaaaa aagataccac cgataccaaa gaggatgcgt ccgatattat tgactgtccg
     1441 attttttgta aaaaagagac agacggcacc gataaagagc ataggtaggg catagttacc
     1501 taatttaaaa ccgatgagaa aggatgtgac agttgtccca atattagcac ccatgacaat
     1561 cccgatagcc tgacgtaagg ttaagagacc ggcactgacc aggccgactg taataactgt
     1621 tacaccagaa ctagactgaa ttagagcagt catcccaata ccaaccagaa ctccaaagaa
     1681 aggattacta gtatatttgt caatgtaaaa acgaaggcga tctccagcag cttgttgtaa
     1741 accgtctccc atggtcttga tgctatataa gaatagcccc agaccaccta aaaagtgaaa
     1801 taaaatttcc tgccaattaa tggacatttc tttttcctcc gaaaaataat cgcggaatat
     1861 ctcctattct attttaaagg ataaaagtaa atctaacaag tgttaatgtc aagtttgtaa
     1921 aagaaaatta taattgataa gtgtattatt tttaagagtt tttaacctta ttttagattt
     1981 gtgaaaacag ctattgtttt ttgtctaaaa atgttttagg ataaaaatgt gattatcatg
     2041 attctagagt taaaaaacta cagaatataa agcgctttct tgaaaacaac aaaatcaatc
     2101 ttttaggagg agaaaatgaa gaatccattt tttgaaagac gttgtcgtta cagtattcgt
     2161 aagttatcag taggagcctg ctcgctgatg attggtgctg ttttatttgc tggtccagcc
     2221 ttggctgaag aaactgcagt tcctgaaaat agcggagcta atacagagct tgtttcagga
     2281 gagagtgagc attcgaccaa tgaagctgat aagcagaatg aaggggaaca tactagagaa
     2341 aacaagctag aaaaggcaga aggagtagcg acagcatctg aaactgcaga agcagctagc
     2401 gcagctaaac cagaggaaaa agcaagtgag gtggttgcag aaacaccatc tgcagaagca
     2461 aaacctaagt ctgacaagga aacagaagca aagcccgaag caactaacca aggggatgag
     2521 tctaagccag cagccgaagc taataagact gaaaaagaag tccagccaga tgtccctaaa
     2581 aatacagaaa aaacattaaa accaaaggaa atcaaattta attcttggga agaattgtta
     2641 aaatgggaac caggtgctcg tgaagatgat gctattaacc gcggatctgt tgtcctcgct
     2701 tcacgtcgga caggtcattt agtcaatgaa aaagctagca aggaagcaaa agttcaagcc
     2761 ttatcaaaca ccaattctaa agcaaaagac catgcttctg ttggtggaga agagttcaag
     2821 gcctatgctt ttgactattg gcaatatcta gattcaatgg tcttctggga aggtctcgta
     2881 ccaactcctg acgttattga tgcaggtcac cgtaacgggg ttcctgtata cggtacactc
     2941 ttcttcaact ggtctaatag tattgcagat caagaaagat ttgctgaagc tttgaagcaa
     3001 gacgcagatg gtagcttccc aattgcccgt aaattggtag acatggccaa gtattatggc
     3061 tatgatggct atttcatcaa ccaagaaaca actggagatt tggttaaacc tcttggagaa
     3121 aagatgcgcc agtttatgct ctatagcaag gaatatgctg ctaaggtaaa ccatccaatc
     3181 aagtattctt ggtacgatgc catgacctat aactatggac gttatcatca agatggtttg
     3241 ggagaataca actaccaatt catgcaacca gaaggagata aggttccggc agataacttc
     3301 tttgctaact ttaactggga taaggctaaa aatgattaca ctattgcaac tgccaactgg
     3361 attggtcgta atccttatga tgtatttgca ggtttggaat tgcaacaggg tggttcctac
     3421 aagacaaagg ttaagtggaa tgacatttta gacgaaaatg ggaaattgcg cctttctctt
     3481 ggtttatttg ccccagatac cattacaagt ttaggaaaaa ctggtgaaga ttatcataaa
     3541 aatgaagata tcttctttac aggttatcaa ggagacccta ctggccaaaa accaggtgac
     3601 aaagattggt atggtattgc taacctagtt gcggaccgta cgccagcggt aggtaatact
     3661 tttactactt cttttaatac aggtcatggt aaaaaatggt tcgtagatgg taaggtttct
     3721 aaggattctg agtggaatta tcgttcagta tcaggtgttc ttccaacatg gcgctggtgg
     3781 cagacttcaa caggggaaaa acttcgtgta gaatatgatt ttacagatgc ctataatggc
     3841 ggaaattccc ttaaattctc tggtgatgta gccggtaaga cagttcagga tgtgagactt
     3901 tattctacta agttagaagt aactgagaag accaaacttc gtgttgccca caagggagga
     3961 aaaggttcta aagtttatat ggcattctct acaacttcag actacaaatt cgatgatgca
     4021 gatgcatgga aagagctaac cctttctgac aactggacaa atgaagaatt tgatctcagc
     4081 tcactagcag gtaaaaccat ctatgcagtt aaactatttt tcgagcatga aggtgctgta
     4141 aaagattatc agttcaacct cggacaatta actatctcgg acaatcacca agagccacaa
     4201 tcgccgacaa gcttttctgt agtgaaacaa tctcttaaaa atgcccaaga agcggaagca
     4261 gttgtgcaat ttaaaggcaa caaggatgca gatttctatg aagtttatga aaaagatgga
     4321 gacagctgga aattactaac tggctcatct tctacaacta tttatctacc aaaagttagc
     4381 cgctcagcaa gtgctcaggg tacaactcaa gaactgaagg ttgtagcagt cggtaaaaat
     4441 ggagttcgtt cagaagctgc aaccacaacc tttgattggg gtatgactgt aaaagatacc
     4501 agcctaccaa aaccactagc tgaaaatatc gttccaggtg caacagttat tgatagtact
     4561 ttccctaaga ctgaaggtgg agaaggtatt gaaggtatgt tgaacggtac cattactagc
     4621 ttgtcagata aatggtcttc agctcagttg agtggtagtg tggatattcg tttgaccaag
     4681 ccacgtaccg ttgttagatg ggtcatggat catgcagggg ctggtggtga gtctgttaac
     4741 gatggcttga tgaacaccaa agactttgac ctttattata aagatgcaga tggtgagtgg
     4801 aagctagcta aggaagtccg tggcaacaaa gcacacgtga cagatatcac tcttgataaa
     4861 ccaatcactg ctcaagactg gcgcttgaat gttgtcactt ctgacaatgg aactccatgg
     4921 aaggctattc gtatctataa ctggaaaatg tatgaaaagc ttgatactga gagtgtcaat
     4981 attccgatgg ccaaggctgc agcccgttct ctaggcaata acaaggtaca agttggcttt
     5041 tcagatgtac cggctggagc aactattact gtttatgata atccaaattc tcaaactccg
     5101 cttgcaacct tgaagagcga agttggagga gacctagcaa gtgcaccatt ggatttgaca
     5161 aatcaatctg gtcttcttta ttatcgtacc cagttgccag gcaaggaaat tagtaatgtc
     5221 ctagcagttt ccgttccaaa agatgacaga agaatcaagt cagtcagcct agaaacagga
     5281 cctaagaaaa caagctacgc cgaaggggag gatttggacc ttagaggtgg tgttcttcga
     5341 gttcagtatg aaggaggaac tgaggacgaa ctcattcgcc taactcacgc aggtgtatca
     5401 gtatcaggtt ttgatacgca tcataaggga gaacagaatc ttactctcca atatttggga
     5461 caaccggtaa atgctaattt gtcagtgact gtcactggca aagacgaagc aagtccgaaa
     5521 actattttgg gaattgaagt aagtcaggaa ccgaaaaaag attacctagt tggtgatagc
     5581 ttagacttgt ctgaaggacg ctttgcagtg gcttatagca atgacaccat ggaagaacat
     5641 tcctttactg atgagggagt tgaaatttct ggttacgatg ctcaaaagac tggtcgtcaa
     5701 accttgacgc ttcattacca aggccatgaa gttagctttg atgttttggt atctccaaaa
     5761 gcagcattga acgatgagta cctcaaacaa aaattagcag aagttgaagc tgctaagaac
     5821 aaggtggtct ataactttgc ttcaccagaa gtaaaagaag ctttcttgaa agcaattgaa
     5881 gcggccgaac aagtgttgaa agaccatgaa actagcaccc aagatcaagt caatgaccga
     5941 cttaataaat tgacagaggc tcataaagct ctgaatggtc aagagaaatt taaggaagaa
     6001 aagacagaac ttgatcgctt aacaggtgag gttcaagaac tcttggctgc caaaccaaac
     6061 catccttcag gttctgccct agctccgctt cttgagaaaa acaaggcctt ggttgaaaaa
     6121 gtagatttga gtccagaaga gcttacaaca gcgaaacaga gtctaaaaga tctggttgct
     6181 ttattgaaag aagacaagcc agcagtcttt tctgatagta aaacaggtgt tgaagtacac
     6241 ttctcaaata aagagaagac tgtcatcaag ggtttgaaag tagagcgtgt tcaagcaagt
     6301 gctgaagaga agaaatactt tgctggagaa gatgctcatg tctttgaaat agaaggtttg
     6361 gatgaaaaag gtcaagatgt tgatctctct tacgcttcta ttgtgaaaat cccaattgaa
     6421 aaagataaga aagttaagaa agtatttttc ttacctgaag gcaaagaggc agtagaattg
     6481 gcttttgaac aaacggatag tcatgttatc tttacagcac ctcactttac tcattatgcc
     6541 tttgtttatg aatctgctga aaaaccacaa cctgctaaac cagcaccaca aaacacagtc
     6601 cttccaaaac ctacttatca accggcttct gatcaacaaa aggctcctaa attggaagtt
     6661 caagaggaaa aggttgcctt tcatcgtcaa gagcatgaaa atgctgagat gctagttggg
     6721 gaacaacgag tcatcataca gggacgagat ggactgttaa gacatgtctt tgaagttgat
     6781 gaaaacggtc agcgtcgtct tcgttcaaca gaagtcatcc aagaagcgat tccagaaatt
     6841 gttgaaattg gaacaaaagt aaaaacagta ccagcagtag tagctacaca ggaaaaacca
     6901 gctcaaaata cagcagttaa atcagaagaa gcaagcaaac aattgccaaa tacaggaaca
     6961 gctgatgcta atgaagccct aatagcaggc ttagccagcc ttggtcttgc tagtttagcc
     7021 ttgaccttga gacggaaaag agaagataaa gattaaatat cgaaaaatct tgtgaaatct
     7081 ttccttatat ttccaaagtg tgatataata gtttcgaata aaataaataa aggagttttt
     7141 gtaacatggc aaaacttact gttaaagacg ttgacttgaa aggtaaaaaa gtcctcgttc
     7201 gtgttgactt caacgtacca ttgaaagatg gcgtaatcac taacgataac cgtatcacag
     7261 cagctcttcc aactattaag tacatcatcg aacaaggtgg acgtgcaatt cttttctctc
     7321 accttggacg tgtgaaagaa gaagctgata aagctggtaa atcacttgct cctgtagcag
     7381 ctgacttggc agcaaaactt ggtcaagatg ttgttttccc aggtgtcact cgtggtgctg
     7441 aattggaagc ggcaatcaac gttcttgaag atggacaagt tctcttggtt gaaaacactc
     7501 gttacgaaga tgttgacggc aagaaagaat ctaaaaacga tcctgaactt ggtaaatact
     7561 gggcatcact tggagatggt atcttcgtaa acgatgcatt cggtacagct caccgtgcac
     7621 acgcatctaa cgttggtatc tcagcaaacg ttgaaaaagc agttgctggt ttccttcttg
     7681 aaaacgaaat tgcctatatc caagaagcag ttgaaactcc agaacgtcca ttcgtggcta
     7741 tccttggtgg ttcaaaagtt tcagacaaga tcggtgttat cgaaaacttg cttgaaaaag
     7801 ctgataaagt ccttatcggt ggtgggatga cttacacatt ctacaaagca caaggtatcg
     7861 aaatcggtaa ctcacttgta gaagaagaca aattggatgt tgcgaaagct cttcttgaaa
     7921 aagcaaatgg taaattgatc ttgccagttg actcaaaaga agctaacgca tttgctggtt
     7981 acactgaagt gcgtgacact gaaggtgaag cagtttctga aggcttcctt ggtcttgaca
     8041 tcggtccaaa atctatcgcc aaatttgacg aagctttgac tggtgccaaa acagttgtat
     8101 ggaacggacc tatgggtgta tttgaaaacc cagatttcca agctggtaca atcggtgtga
     8161 tggacgctat cgtgaaacaa ccaggagtta aatcaatcat cggtggtggt gactcagctg
     8221 ccgcagcgat taaccttggc cgtgcagaca agttctcatg gattagtacg ggtggtggag
     8281 catcaatgga acttcttgaa ggtaaggttc ttccaggact tgcagccttg acagaaaaat
     8341 aagattttat aaacaaatca aagaagagag ggatgaaagt tcctcttttc ttttgcttaa
     8401 aataaaaacg cttcctctca actattactc ataaaaatca ccgatttatg ataaaatgga
     8461 aatagaaagt tgagattatg agttatttta aaaaatataa attcgataaa tcccagttca
     8521 aacttggtat gcgaaccttt aaaacaggta ttgctgtttt tctagttctc ttgatttttg
     8581 gcttttttgg ctggaaaggt cttcaaattg gtgctttgac agccgttttt agcctgaggg
     8641 agagttttga tgagagtgtt cattttggga cttcgcgtat tctaggaaat agtatcggtg
     8701 gactctatgc cttggtcttc ttcttattaa ataccttttt ccacgaagcc ttttgggtga
     8761 ccttggtagt tgttccaatc tgcaccatgt taaccattat gacaaatgta gccatgaata
     8821 acaaagcagg ggttattggt ggtgtagcag ctatgttaat cattacccta tcaattccaa
     8881 gtggtgagac aattttgtac gtgtttgtgc gtgtattaga aacgtttatg ggagtttttg
     8941 tcgcaattat cgtaaattac gatattgatc gtattcgtct ctttttagag aaaaaagaaa
     9001 aataatgtta cattttataa cattatcaat tgacgtttgt ctttttttag actataatag
     9061 acagaaagaa ggaaattgta aatgaaggaa aaagaatttc gccgaaatat ggctgttttt
     9121 cctatcggca gtgttatgaa gttgaccgat ctatcggcgc gtcagattcg ttattatgaa
     9181 gatcaagagt tgatcaagcc cgatcgaaac gaaggaaatc gtcgcatgta ttccttgaat
     9241 gacatggatc gtctgcttga aatcaaagat tatatctctg aaggttataa tatcgctgcc
     9301 attaagaaaa aatatgctga acgtgaagcg aaatccaaga aagcggttag tcagactgaa
     9361 gtacgtcgtg cacttcacaa tgaactcctc caacaggggc gttttgcttc agtacagtca
     9421 ccttttggtc gcggttaggc aaccgcaagt agtcatagat taaggagaaa acttatgcca
     9481 atcacagctg cagatattcg tcgtgaagtc aaggaaaaaa atgttacctt tattcgtctt
     9541 atgttctcag atattttggg aaccatgaaa aacgtcgaaa ttcctgctac agatgaacag
     9601 ttagataagg tcttgtcgaa caaggttatg tttgatggat cttctattga aggttttgta
     9661 cgtatcaatg agtcggatat gtacttgtac ccggacttgg atacatggac agtcttccct
     9721 tggggagatg aaaatggaag tgttgcaggt ctgatctgtg atgtttatac aacagaaggt
     9781 gaaccatttg cgggtgaccc tcgtggtaat ttgaaacgag ctcttcgtca catggaagaa
     9841 gttggattca aatccttcaa ccttggtcca gagccagaat tcttcctatt taagttggat
     9901 gaaaatgggg acccaacact tgaagtgaat gacaagggtg gctactttga tttggcacct
     9961 actgaccttg cggacaacac acgtcgtgag attgtgaatg tcttgaccaa aatgggattt
    10021 gaagtagaag cgagtcacca cgaggttgcg gttggacagc atgagattga ctttaagtac
    10081 gatgaagttc tccgtgcttg tgataagatt caaatcttta aacttgttgt taaaaccatt
    10141 gctcgcaaac acggacttta cgcaacattt atggcgaagc caaaatttgg tattgctggc
    10201 tcaggtatgc actgtaatat gtccttgttt gatgcagaag gaaataacgc cttctttgat
    10261 ccaaatgatc caaaaggaat gcagttgtca gaaacagctt accatttcct aggcggtttg
    10321 atcaagcatg cttacaacta tactgccatc atgaacccaa cagttaactc atacaaacgt
    10381 ttggttccag gttatgaagc gcctgtttac attgcttggg ctggtcgtaa ccgttcgcca
    10441 cttgtgcgcg tacctgcttc acgtggtatg ggaactcgtc ttgagttgcg ttcagtggat
    10501 ccaatggcga acccttacat tgctatggct gttcttttgg aagttggttt gtatggtatt
    10561 gaaaataaaa tcgaagcacc agctcctatc gaagaaaata tctacatcat gacagcagaa
    10621 gagcgcaagg aagctggtat tacagacctt ccatcaactc ttcacaacgc tttgaaagct
    10681 ttgacagaag atgaagtggt taaagctgct ctcggagatc acatctacac tagcttcctt
    10741 gaagccaaac gaatcgaatg ggcaagttat gcaaccttcg tttcacaatg ggaaattgat
    10801 aattatttag acctttacta atatagaaga aagaggctct ttgtcaactg tagtgggttg
    10861 aaaaaaagct aagcttgaga aaggacaaat tttgtccttt cttttttgat attcagggcg
    10921 at
//
LOCUS       DAVKGU010000063        10853 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKGU010000063 DAVKGU010000000
VERSION     DAVKGU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.063"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            1..785
                     /locus_tag="VW077_001510"
                     /pseudo
     CDS             1..785
                     /locus_tag="VW077_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            685..1131
                     /locus_tag="VW077_001511"
     CDS             685..1131
                     /locus_tag="VW077_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9455509.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTRKPAHSPILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1268..2572)
                     /gene="eno"
                     /locus_tag="VW077_001512"
     CDS             complement(1268..2572)
                     /gene="eno"
                     /locus_tag="VW077_001512"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9455510.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2736..3182
                     /locus_tag="VW077_001513"
     CDS             2736..3182
                     /locus_tag="VW077_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9455511.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3179..4294)
                     /locus_tag="VW077_001514"
     CDS             complement(3179..4294)
                     /locus_tag="VW077_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9455512.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     VSGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGILDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4303..4632)
                     /locus_tag="VW077_001515"
     CDS             complement(4303..4632)
                     /locus_tag="VW077_001515"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU9455513.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCAKEVLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4577..4948)
                     /locus_tag="VW077_001516"
     CDS             complement(4577..4948)
                     /locus_tag="VW077_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9455514.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(5007..6440)
                     /gene="glgA"
                     /locus_tag="VW077_001517"
     CDS             complement(5007..6440)
                     /gene="glgA"
                     /locus_tag="VW077_001517"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9455515.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6437..7576)
                     /gene="glgD"
                     /locus_tag="VW077_001518"
     CDS             complement(6437..7576)
                     /gene="glgD"
                     /locus_tag="VW077_001518"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9455516.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLSFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7566..8708)
                     /locus_tag="VW077_001519"
     CDS             complement(7566..8708)
                     /locus_tag="VW077_001519"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9455517.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8698..10626)
                     /gene="glgB"
                     /locus_tag="VW077_001520"
     CDS             complement(8698..10626)
                     /gene="glgB"
                     /locus_tag="VW077_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9455518.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKNFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNPKGVENEK"
BASE COUNT         3327 a         2374 c         1924 g         3228 t
ORIGIN      
        1 ctcacgtaat accatttatg gctggttaaa gctaaaaaag aaaacaggag agctaagcca
       61 ccaagtaaaa ggaacaaaac caagaaaagt tgatagagat agacttaaaa actatcttac
      121 tgacaatcca gacgcttatt tgactgaaat agcttctgac tttggctgtc atccaactac
      181 catccactat gcgctcaaag ctatgggcta cactcgaaaa aaagaaccac acctactatg
      241 aacaagaccc agaaaaagta gccttatttc ttaagaattt taatagttta aagcacctag
      301 cacctgttta gattgacgaa acaggattcg atacttattt ttatcgagaa tatggtcgct
      361 cattaaaaga tcagttaata agaggcaaag tatctggaag aagatatcag aggatttctt
      421 tggttgcagg tctaacaaat ggtgagttaa tcgctccaat gacttacgaa gagacgatga
      481 cgagcgactt ttttgaagct tggtttcaga aatttctctt accaacatta accacaccat
      541 cggttattat tatggataat gcaagattcc atagaatggg gaagaatgta agattccata
      601 gaatggggaa tctagaactt ttatgcgaag agtttgggca taaactttta cctcttcctc
      661 cctactcgcc tgagtacaat cctattgaga aaacatgggc tcatatcaaa aagcacctca
      721 aaaaggtatt accaagttgc aatacctttt acgaggctct tttgtcttgt tcttgtttca
      781 attgactata taaattgtct aagcgaaaca accgataaga attggcacaa aagcgaccgt
      841 atttttgtta ccaatacaag aaaaccagct catagtccta tcttgagtaa gtctctccag
      901 cgagcaaacg aacgccttaa aaaaccaatt cccaaacatc tgtcccctca catcttcaga
      961 cacaccacta ttagcatctt atcagaaaat aaaattcctt taaaaacaat cacggacagg
     1021 gttggtcatt ccgactctga agtcactact tccatctaca cccacgtcac aaagaacatg
     1081 aaagatgaag caatcaatgt actggataaa gttatgaaaa agatttttta aaagttttgt
     1141 cccttttttg tcctctaaat acaaaaatag cccttcggat aaaatccgag gggctagaaa
     1201 cgttgttaaa tcaacggccg aacttttgaa tttcatggtt cgggataaaa tagttcactg
     1261 aactatttta ttttttaagg ttgtagaatg atttcaatcc acgatattca gctacttcac
     1321 caagttggtc ttcgatacga agcaattggt tgtatttagc gatacggtct gtacgtgaaa
     1381 gtgaaccagt cttgatttgt cctgcgttag ttgcaactgc gatatcagcg attgttgaat
     1441 cttcagtttc acctgaacgg tgtgatacaa cagcagtgta accagcttct ttagccattt
     1501 cgatagcttc aaaagtttca gtaagagtac cgatttggtt aactttgata aggattgagt
     1561 tagcagcacc ttcttggata ccacgtgcaa ggtagtcagt gtttgttacg aagaagtcgt
     1621 caccaacaag ttgtactttc ttaccaagac gttcagtaag agctttccaa ccatcccagt
     1681 cgttttcatc cataccatct tcaatagtga tgattgggta tttgttaacc aattcttcaa
     1741 ggtagtcgat ttgttctgca gatgtacgaa cagcagcacc ttcaccttca aatttagtgt
     1801 agtcgtaaac tttacgttct ttatcgtaga attctgatga agcacagtca aatccgataa
     1861 atacgtcttt acctggtaca tatccagcag cttcaatcgc agcaaggata gtttcaacac
     1921 catcttcagt tccttcgaaa cgaggagcga atccaccttc gtcacctacg gcagtttcca
     1981 aaccacgtga tttaaggatt ttcttaagag cgtggaagat ttcagcaccg taacgaaggg
     2041 cttctttaaa tgttggcgca ccaactggca agatcatgaa ctcttggaaa gcgattggag
     2101 cgtcagagtg agaaccaccg ttgatgatgt tcatcattgg agttggaaga actttagtgt
     2161 tgaatccacc aaggtagctg taaagtggga tttcaaggta gtcagcagca gcacgagcta
     2221 cagcgataga cacaccgagg attgcattcg cacccaattt acctttgtta ggagtaccgt
     2281 caagtgcgat catagcacgg tcaatagctt gttgatcacg tacatcgtag ccaatgatag
     2341 cttcagcaat gatgttgttt acgttgtcaa cagctttttg tgtaccaaga ccaccgtaac
     2401 gagatttgtc accgtcgcga agttcaactg cttcgtgttc accagtagaa gctcctgatg
     2461 gaaccatacc acgtccgaaa gcacctgatt cagtgtaaac ttctacttca agtgttgggt
     2521 taccgcgtga gtctaggact tcgcgagcgt aaacatcagt aataattgac attttttact
     2581 ctccttatga gttaaatttt ttacacctct ataatacctt aaaacccctc ctttttcaag
     2641 aaaaaacgtt atctttgtgc aacttttcct taactttata aagtaatcgc tttcttttgt
     2701 ctgttttatt ctaactttta tgatatactg ttttcatgac agatttatca aaacaattac
     2761 ttgaaaaagc tcatggtggg ttaaaaataa atccggatga gcaaagacgc tatcttggta
     2821 cttttgagga aagagttctt ggatatgtag atattgacac agcaaatagc cctcagttag
     2881 aaaaaggctt tttatttatt ttagaaaacc ttcaggaaaa agcagagcca ctatttgtga
     2941 agatttcacc aactatcgaa tttgataagc aagttttcta cttaaaagaa gcaaaagaaa
     3001 ctgatagtca agccaccata gtatctgaag agcatattac ttctcctttc ggcctggtta
     3061 ttcatagcaa tgcaccagtt caagtagaag aaaaagacct tcgacttgct tttccaaaac
     3121 tttgggaagt taaaaaggaa gaaccagcca aaacatcctt atggaagaaa tggtttagct
     3181 aaatcttgca catatttaat aagtgcccaa tattggcagc cgtgcgctcc agatagagac
     3241 tggcattttt caaactatct tctaaaggtt cacttttctc caaaatagaa aagacagctt
     3301 ggatattttc aaatggtagg ggaggtaaat cttcagcaag actaccgcaa atagcaataa
     3361 caggaactcc aacaggggtt ctttttgcaa cacctatagg cgctttccca gcaaagcttt
     3421 gactgtcaag tattccttct ccaacaacaa ccaagtcagc atctgaaact ttcttatcaa
     3481 agttgattaa gtccaagcag gtatcaattc cagacacgat acttgcctga gcaaaggcac
     3541 acaaaccacc agcaaggcct ccacctgctc ctgctccttt aatttctaat gttgcaggtg
     3601 agaatttttc ataaaaatct tggatcgcct gatctacgac tgcaaacata gtcggatgta
     3661 gacctttttg attgccaaaa gtgtaagtcg caccttgatg accacataag ggactcacga
     3721 catctgctag aatatgaatt tgaacacctt caggaatttt atagcaattt tctgttgaaa
     3781 cagaagctaa gtttaataag gattgaccgg aaacaggcaa gacatttcca tccctatcat
     3841 aaaattgata acctaaacca gcagcaatcc ccagtcctcc atcattactg gccgtgccac
     3901 caacaccgat ataaatatct ttaatccctt tagaaatgag atggagaatc aactctccaa
     3961 taccacaagt ttggatttga agtggatttc gtttctctag cggaattttt ccaagaccaa
     4021 ccaagtcagc tacttcaaat agtgccagtt cccctttttg aaaatagcgc atggcttctt
     4081 tttgtccaaa agggtctgtc acttggatcc atttttcttt taggtcaaga gaatgtcgga
     4141 tagcatctac agtaccttct cccccatcac caacagggca gaggagacat tctacatctg
     4201 ctatcgattg ttggaagcct ctttttattg cttcagctac ctgttgagct gtcaagcttt
     4261 ccttaaacga atccggtgca attacaatct tcatattttc cctcattcta aacagtcaat
     4321 caaaggaaga acttctaaaa aatccctctt gtcaacatga tgtggtattt cttttttgag
     4381 cacttctttg gcacaaaagg cgattcctaa ctccgccgac ttcaacatta atagattatt
     4441 gaccccatca ccgattgcca ccgttctttc tttagaaagt tttagtttct ttctccattt
     4501 ttccagagtt tcttttttga cctggggact tataatttgt ccaactaatt ttcctgttaa
     4561 aagaccttct ttgacttcaa gctggttggc agtgaaatag gcaataccaa gggattttgc
     4621 taatctctcc aactattggt gtaaatccac cagacaccag accaactagg atgccattct
     4681 tttggagaat agagatgaac tctgggacat ttagcgatag atgaattgag ttgaagacgt
     4741 tatcaaagac caaaatagga agaccttcca acaaggacac tctttttctt aaactgcttt
     4801 caaagaccaa ctctcctcgc attgctcgac ttgtaatctg cgaaatttcc gcctcatgac
     4861 ctgcctctct ccctaaaaga tcaatcactt cttctaggat taaggttcca tctacatcca
     4921 aaacacacaa gccttttact tgagacatca gttctcctct ctaaacagcc taaaaatcgt
     4981 atgaagtcat catacgattt tatctattaa ttaactaaac tatggtacaa gtcaaggtat
     5041 gacttgcagg ctgtatccca tgagaagtca ctctccatag cttgtttttg taggtttctc
     5101 caaatgtctg gatggtttct atacaagtcc aatgctgttt ggaaagtcca atttaaccaa
     5161 taaggagata gattgtcaaa gctaaagcca gtaccgcttc cttcgattgg attgaaagcg
     5221 cgaactgtat ctcgcaagcc tccaacttca tggaccaatg gcaaggttcc ataacgcata
     5281 gccatcattt gagacaagcc acacggttca aaacgacttg gcatgaggaa gaggtcacaa
     5341 gcagcgtaga tttcctgagc aagtttgaca tcaaaagtga tatttgttga tagcttgtct
     5401 gggtaaatct gagcaaacca tgagaaagct ccttcaaagg ctggatcgcc agttcccaaa
     5461 agaacaatct gaacatcttc ttgcaagata tggtgaagac tttcgaccac cacatcaaaa
     5521 cctttttgac gtgtcaaacg agaaacaatt cccaccagtg gaacgtctgc tctaacaggc
     5581 aagccaactc tttcttgcaa ttttgcctta tttttggctt tcccagacaa atcttcctga
     5641 ttgaaatgat agtctaaaag agcatccgtc tgaggattat aaagatcagc atcaatccca
     5701 ttcacgatac cagatacttt accagactcc attttaagaa tctgatccaa attacatcca
     5761 aactgactag tcataatttc atgagcatag ctaggtgaaa cggttgaaac acggttcgca
     5821 tagagaatac ctgccttcat ccagttcaga cagttgttcc atcgaagggt gccatcagcg
     5881 taacgttcaa ggccaactcc aaacaaatca cccaacattc cttctgaaaa ttgtccttgg
     5941 aattctaaat tatgaatggt taaaactgtt tcaatgtcct cataggcttg aatccaacgg
     6001 tatttttcct tcaacaagaa aggaatcata gctgtatggt agtcatgaac atggagaaga
     6061 tcaggaataa agtcaatcct ttccatagcc tcaatggcag ccagttggaa aaaggcaaag
     6121 cgttctccgt catcaaaatc accgtaaaca tgaccacgga agaaataata ttgattgtca
     6181 ataaagtaga aggttacacc atttaatact gttttcttaa ttccacaata ctgtctgcgc
     6241 caaccaacgc tcacctcaaa atgaagcaca tcttcaatct gatttccaaa tttagcctct
     6301 accatatcat agtagggtaa aatcactgca acttcgtgcc cagcttttac cagtgatttt
     6361 ggaagagcgc caatgacgtc tcccaaacca cctgtttttg aaaagggtgc accctctgct
     6421 gctacaaata aaattttcat gaatgaatat cctctgttac tttagcacct ttcttaacca
     6481 caactggatg ttctgcagtt cctcgaatca caacaccagg ctcaacttca acccctttgt
     6541 ccaagatagc atattcgacc tgagcccctt ctccaataac aacacgaggg aagagcaggc
     6601 tatctttaac caagctatcc ttatggacat gaatattacg tgatagaaca gaattagcta
     6661 cttgaccttc aataatacta ccagaggcaa actgagaagt gcttacctta gatgtattag
     6721 catagtaagt tggctcttcg tttttgacct ttgtataaat cttttggttt ggtgagaaaa
     6781 gagaatagaa tttttgtgat tcaagcatat cgatattcgc ttgataataa gatttaacag
     6841 agtgaatatt ggctagatag cccgtgtact cgtaggcgaa agctccctct tttacagcca
     6901 aatcccgtaa aacatagcgc aatttctctg gatgttcttt tttagcttct tcttccaagt
     6961 gttcaatcaa ccaaggtgta tcaacgacaa agatatctgt agacatattg aacgtttcag
     7021 ctgttgactt gctatcaaag agtttatgag aaagaacatg gtctgtttca tctacatcca
     7081 agattgcatt tacttctgaa atatctttct tagctagttt tttataaact acagtgatag
     7141 gctcttttgt tgtactatgt aggtggaaaa cttggttcaa atcaatgtta ataagaacat
     7201 cgcagttgag ggcaaccgtt tggtttgagc cagaacgttt caaataagta agaagctgtt
     7261 ggtagtattc ttttccaact gtactacttt ctacacgggt attgtaaatt cctagatagt
     7321 aatggctaag aagggttgat aagccccact cgcgtcctga acgaatatgg tcaaatactg
     7381 agctgatatt atcctgctgg aaaataccaa agacactacg aacacctgca ttagcaaggc
     7441 ttgaaagtgg gaagtcaatc aaacgatatt tcccaccaaa tgacaaactt gctactggac
     7501 ggtggtccgt caatgtcgac atattgtgaa aaccaactgt atttcctaaa atggcagaat
     7561 atttatcaat cttcatctgt tgctaccccc actacttcat tatatcctac aacttgtact
     7621 tcatctgttc catcaatttc gacaccgtca gaaataatcg caccttcacc aataatggca
     7681 cgtttaatct tagctccttg accaatgata gctccactca tgataactga atcaaggact
     7741 tccgctcctt cgcgaacttg cgcgcctgtt gaaaggatag aatgtttaac agttccatca
     7801 acgaaacatc cgtctacaac taatgagtct tccacatgag catttgcccc gaggaagttt
     7861 ggtggtgaaa tcaagtttct tgagtaaatc ttccattgac ggttacgact atccaaggca
     7921 ttttctggag aaatatactc catgttcgct tcccaaagtg actcaatagt accaacatct
     7981 ttccaataac cactaaattc gtaagcataa acactttcac ctgactcaag gtaatttgga
     8041 atgacatttt taccaaagtc tgacatgcca accttgctct tttcagcagc gactaacata
     8101 ttacgaaggc gttgccaatc aaaaatgtag attcccatag aagcttttgt agatttaggt
     8161 tgagctggtt tttcttcaaa ttcaacaata cgattgttag catctgtgtt catgatacca
     8221 aaacggcttg cttctttaag agggacgtct aaaactgcta ctgtcaagct ggcattatta
     8281 tccttatgag actggagcat atcatcatag tccattttgt agatgtggtc cccagacaaa
     8341 atcaagacat actcaggatt gacactgtcg atatagtcga tattttggta aatagcgtga
     8401 ctagttccct caaaccaacg atttccttca cttgcagaat aaggttgaag aatagagaca
     8461 cctgaattaa taccgtctag tccccagctt gaaccattcc caatatggtt gttgagagca
     8521 agtggttgat actgtgtaac gaccccaaca ttatgaatcc ctgagttggc acagtttgat
     8581 agggcaaagt caatgatacg gtagcgccca ccaaattgca cagctggttt tgcgatgctt
     8641 tgagtgagtt taccgagacg agttccttgc ccaccagcaa gaatcaaagc taacatttca
     8701 tttttcattt tctactcctt ttgggttttt atttgtgacg gttttagtag atttcaagcg
     8761 acgtttgatt ttccatacac ttgctcccat agccggtagg gtaaaggtta aggtctgctc
     8821 ataatctttc catagtcctt cttgcgtttg aacagtttga ttatgttctt tccaaacgcc
     8881 tccccactct tccaactcag tattccatac ttcttcgtaa attcctgcaa cgggtagtcc
     8941 gattgtaaaa tttttccgct caacaggtac catgttaaag atacagacta acatttctcc
     9001 ctttttaccc ttacgaataa aggaaagaac actctggtct cgattatccg catcaatgat
     9061 ttcaatacca tcatagctgg tatcgatttc ccacagacag cgatgatctt tgtaaaactg
     9121 gtttagctga gaagtgaaat acttcatctt agcattcatt gggtcttcta ggttagacca
     9181 ttccaactgt tcttcagatt tccattctag gaattgaccg tattcgctac ccatgaagag
     9241 caatttctta ccagggtgac aaatttggta cgtatagaga ttgcgcaagc ctgcgaattg
     9301 attgtaacga tctccccaca tcttatgcat catactcttc ttgccatgaa ccacttcatc
     9361 gtgcgagaat ggcaagagat aattctcctt gaaaacatac ataaagctga aagtcaccag
     9421 gttaaagtca tatttacgat aaatcggatc ttcttcgtag aaacggagga tatcattcat
     9481 ccagcccatg ttccatttgt agtcaaatcc tagaccacca atctctttca ttcccgtaat
     9541 cttggttgca gacgaacttt cttctgcaat catcatcaca tctggatatt ctaacttaat
     9601 aacttcattc aagcgttgaa ggaaataata accttcatag ttgagatttc cgccatcttt
     9661 attaggtgtc catggagcat catcatagtc caaatagagc atgttgctaa cagcatccac
     9721 acgaatacca tccaaatgat agacatcaat ccaatgctta atgcaagaaa ttaagaagga
     9781 ctggacttca ttttttccaa ggtcaaaatt aagggcaccc caaccatggt tatgagcctt
     9841 attatggtct tggtattcaa aagtcggtgt cccatcataa taagctaagg catcatcgtt
     9901 gatggtaaag tgacctggta cccagtccac aataacccca atattatggg tatgacactc
     9961 ctcgacaaaa tcttgaaact cctctggtcg gccataagca tgctctaaag cgaagtaacc
    10021 cataagctga tacccccaac tcaagcccaa aggatgggac atcaagggca taaactcaat
    10081 atgagtatag ttcatttcaa cgagataagg aatgagttca tccttgagct gggcaaaact
    10141 ataaggactg ccatcagaat ttcttttcca tgatccagcg tgaacttcat aaatattgac
    10201 aggacgctct tcaaagcccc aacgttttct tcgtgccagc caaagtccat ccttccattt
    10261 cttctcagga agctctgtta agattgcccc tgttcctgga cgagcctcat atctgacagc
    10321 aaaagggtca atcttcatca gttgatgacc attttgacgt gtgacatgat atttgtaaat
    10381 atgcccttct tgagccatat tggtaaagac ttcccagacc ccaaaatcat ttcttaccat
    10441 tggaatctga ttttcaatcc agttggtaaa atcaccaacc aagtgaacag cctgagcatt
    10501 aggtgcccaa acacggaagg tatagccatg ctctccattt agttcttccc tatgtgctcc
    10561 tagataatgt tggagataaa aattttcacc cgtcataaag gtttttaatg cttctctatt
    10621 atccatatac tccccttctc ctgtaagcgt tttctatgct tttattatac taccttttta
    10681 gagaagattc aagtaaatta ctatacttct ttaattattt tgaaaatcta caacaagttc
    10741 acttactcgt tcaattgtaa atcaatattt tttcaaaaaa ttgcgaaaac gcctttcttt
    10801 ttctactata gtgaaatgaa ataaaacatg cgcaaatcga ttaaggaatt taa
//
LOCUS       DAVKGU010000064        10751 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKGU010000064 DAVKGU010000000
VERSION     DAVKGU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10751
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.064"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            117..614
                     /locus_tag="VW077_001521"
     CDS             117..614
                     /locus_tag="VW077_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9455519.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            614..2269
                     /gene="dnaX"
                     /locus_tag="VW077_001522"
     CDS             614..2269
                     /gene="dnaX"
                     /locus_tag="VW077_001522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9455520.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYTPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQIEKEVEESLIPEGFEFLADKVKVEED"
     gene            2297..2491
                     /locus_tag="VW077_001523"
     CDS             2297..2491
                     /locus_tag="VW077_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9455521.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2641..3411
                     /gene="sufC"
                     /locus_tag="VW077_001524"
     CDS             2641..3411
                     /gene="sufC"
                     /locus_tag="VW077_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9455522.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3439..4701
                     /gene="sufD"
                     /locus_tag="VW077_001525"
     CDS             3439..4701
                     /gene="sufD"
                     /locus_tag="VW077_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9455523.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLLGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4712..5938
                     /locus_tag="VW077_001526"
     CDS             4712..5938
                     /locus_tag="VW077_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9455524.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINACSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5925..6365
                     /locus_tag="VW077_001527"
     CDS             5925..6365
                     /locus_tag="VW077_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9455525.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDVVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6419..7831
                     /gene="sufB"
                     /locus_tag="VW077_001528"
     CDS             6419..7831
                     /gene="sufB"
                     /locus_tag="VW077_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9455526.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8062..9303)
                     /locus_tag="VW077_001529"
     CDS             complement(8062..9303)
                     /locus_tag="VW077_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9455527.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9406..10515
                     /locus_tag="VW077_001530"
     CDS             9406..10515
                     /locus_tag="VW077_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9455528.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLIWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYVLVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPQIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3194 a         1965 c         2332 g         3260 t
ORIGIN      
        1 tccataatat ctatagtgga tttacccact acaaatatta tagagcccaa aatcctttgt
       61 ttactaaaca agggattttt cttttgtctc tgctcctttt ttgatataat agttctatgt
      121 taaaatcaga aaaacaatca cgttatcaaa tgttaaatga agaattgtcc ttcctattgg
      181 aaggcgaaac caatgttttg gctaatcttt ccaacgccag tgctctcata aaatcacgtt
      241 ttcctaatac cgtatttgca ggcttttatt tgttcgatgg aaaggaattg gttttaggtc
      301 ccttccaagg aggtgtttcc tgcatccgta ttgcactagg caagggtgtt tgtggtgagg
      361 cagctcactt tcaggaaact gttattgttg gagatgtgac gacctatctc aactatattt
      421 cttgtgatag tctagctaaa agtgaaattg tggtgccgat gatgaagaat ggtcagttac
      481 ttggagttct ggatctggat tcttcagaga ttgaggatta cgatgctatg gatcgagatt
      541 atttggaaca atttgtcgct attttgcttg aaaagacaac atgggacttt acgatgtttg
      601 aggaaaaatc ttaatgtatc aagcacttta tcgaaaatat agaagtcaaa acttctccca
      661 gttagttggt caagaagttg tggctaagac tcttaaacaa gcggtggagc aagagaaaat
      721 aagtcacgct tatctttttt ctggtcctcg tggaacggga aaaaccagtg ttgctaaaat
      781 ctttgccaag gctatgaact gtcccaatca agtgggtggc gaaccttgca ataactgcta
      841 tatttgtcaa gcagtgacgg acggtagttt agaagatgtc attgaaatgg atgcagcttc
      901 taataatggg gtagatgaaa ttcgcgaaat tcgtgataaa tctacctata cgcctagcct
      961 tgctcgttat aaggtttata tcatagatga ggttcacatg ctgtctacag gggcttttaa
     1021 tgccctccta aagacgctgg aagaaccaac acagaatgta gtctttattt tggccactac
     1081 tgaattgcac aagattcctg ctactattct atcccgtgtg caacgttttg agtttaaatc
     1141 aattaagaca caggatatta aggaacatat tcactatatc ttagaaaaag aaaatatcag
     1201 ttctgaacca gaggctgtgg aaatcattgc cagacgggcg gaaggtggaa tgcgggacgc
     1261 cttgtctatt ttggatcaag ccctgagttt gacacaggga aatgagctga cgactgctat
     1321 ctctgaagaa attactggca ccattagcct atcagccttg gatgattatg tggcggcctt
     1381 gtctcaacag gatgttccca aagctttgtc ttgcttgaat cttctttttg acaatggtaa
     1441 gagcatgact cgttttgtga ccgatctttt gcactattta agagacttgt taattgttca
     1501 aacaggggga gaaaatactc atcatagttc agtctttgta gaaaatttgg cacttcctca
     1561 aaaaaatctg tttgaaatga ttcgcttagc aacagtgagt ttagcagata ttaagtctag
     1621 tttgcagccc aagatttatg ctgaaatgat gaccgtccgt ttggcggaaa tcaagtccga
     1681 accagcttta tcaggagcgg ttgaaaatga aattgctacg ctgagacagg aagttgcccg
     1741 tctcaaacaa gagctttcta atgtaggtgc ggttcctaaa caagttgcac cagctcctag
     1801 tcgaccagct acgggcaaaa cagtctatcg tgtcgatcgc aataaagtgc aatctatctt
     1861 acaagaggcc gtcgaaaatc ctgatttagc acgtcaaaat ttaattcgtt tgcagaatgc
     1921 ctggggagag gtaattgaaa gtctaggtgg gccggacaag gctctgctag ttggttctca
     1981 accggttgct gccaatgaac accatgctat tcttgctttt gagtctaact tcaatgctgg
     2041 tcaaactatg aaacgagaca atctcaatac catgtttggt aatatcctca gtcaggcggc
     2101 aggtttttca cctgagattt tagctatttc catggaggaa tggaaagaag ttcgcgcagc
     2161 cttttcagcc aaagccaaat cttctcaaat tgaaaaagaa gtagaagaaa gcctgattcc
     2221 agaaggattt gaatttttgg ctgataaagt gaaggtagag gaagactaaa gaaagatttc
     2281 atgatacaat aagtttatga ataaacaaca atttattatt atggcgctgt ttacagctgc
     2341 tgagacctat tttttcaatg aagcctggat gactggtcgc tatattatgg cagccttttg
     2401 ggcaatttta ctctttagaa atttccgagt cagttatgtg atgggcaaaa tcgttgatgt
     2461 catcgatcag cattttaata ggaaagacta gccctcagct tccagacaaa atcaaagcct
     2521 tttaggcttt tttttgttat actagaaaag tatatttata gaatttttgc tctatttctg
     2581 gggaaatcag acgtttttct agtaagtact gtaaaagttt tgaaaaagaa aggaactatc
     2641 atgtcagtat tagagatcaa agatcttcac gttgagattg aaggaaaaga aattttaaaa
     2701 ggggttaacc tgaccctgaa aacaggagaa attgccgcta tcatgggacc aaatggtaca
     2761 ggtaaatcga ctctttctgc cgctatcatg ggaaatccaa actatgaagt aactaaaggt
     2821 gaagttttgt ttgatggcgt aaacatcctt gagttggaag tggatgagcg tgcgcgtatg
     2881 ggacttttcc ttgctatgca atacccatca gaaatccctg gaattaccaa tgctgagttt
     2941 cttcgtgccg ctatgaatgc gggtaaagaa gatgatgaga agatttcagt tcgtgagttt
     3001 attactaagc tagatgaaaa aatggaattg ctcaacatga aagaagaaat ggcagagcgt
     3061 tacctcaacg aaggcttctc tggtggtgag aaaaaacgca atgaaattct tcaacttttg
     3121 atgttggagc caacatttgc tcttttggac gagattgact caggtcttga tattgacgct
     3181 cttaaagttg tgtctaaagg tgtcaatgcc atgcgtggtg aaggttttgg tgctatgatc
     3241 atcactcact accaacgtct tttgaactat atcacacctg atgtggtaca cgtgatgatg
     3301 gaaggtcgtg ttgtcctttc tggtggtcca gaattggctg cgcgtttgga acgtgaagga
     3361 tacgcaaaac tagctgaaga acttggctac gactacaagg aagaattgta attccctcgt
     3421 atcttttagg agaagtaaat gactagagaa aatattaaac ttttttcaga aatgcacgct
     3481 gaaccaagct ggttggctga tctccgtcaa aaagcttttg acaagattga gactttggaa
     3541 ttaccagtta ttgagcgtgt caaattccac cgttggaatc tgggtgatgg aacgattaca
     3601 gaaaatgagc catcagcaaa tgttccagat ttcacagctt tagatcatca cttgaagttg
     3661 gtgcaagtag gaactcaaac tgttttcgaa caaactccag ttgagttagc tgaacagggt
     3721 gttgtcttca cagactttca ctcagcttta gaagaaattc cagagctgat cgaagaattc
     3781 ttcatgtcat ctgttaagta tgatgatgac aagttggcgg cttaccacac agcttacttt
     3841 aacagtggtg ctgtactcta tattccagat aacgtagaaa ttacagagcc aattgaagga
     3901 attttctacc aagatagcga tagcaatgtg ccgtttaaca agcatattat gattatcgct
     3961 ggtaaaaatt ctaagattag ttatctggag cgtttagagt cacgcggtga aggaagtgac
     4021 aaagcaactg ccaatatcac agtggaagtg attgcacgtt ctggtgcgca agtcaagttt
     4081 gctgctatcg accgtctagg tgaaaacgtc actgcctaca ttagccgtcg tggtaaatta
     4141 ggcaacgatg caagtattga ctgggctatc ggtgtcatga acgaaggaaa tgtcgttgct
     4201 gattttgata gtgacttgct tggaaatggt agccatgcgg acctcaaggt agtagcactt
     4261 tcaagtggtc gtcaggtgca agggattgat actcgcgtga ctaactatgg ttgcaactct
     4321 atcggaaata tcctacaaca tggggttatc cttgaaaaag caactttgac tttcaatggt
     4381 atcggccaca tcatcaaggg tgctaaggga gcagatgccc aacaagagag ccgtgttctc
     4441 atgctttcag accaagcgcg ttcagatgct aacccaattc ttttgattga tgaaaatgat
     4501 gtcactgcag gacacgcagc gtctatcgga caagttgatc cagaagacat gtattacctc
     4561 atgagtcgtg gattggataa ggcaactgca gagcgtttgg ttgttcgtgg tttccttgga
     4621 tctgttatcg tggagattcc agtcaaggaa gttcgtgatg aaatgattgc aactatcgaa
     4681 gagaaattgt caaaacgcta aggggaagcc tatgttagat gtagaagcga ttcgcaagga
     4741 ttttccaatt ttagatcaga ttgtcaatga tgaacctctg gtctatctgg acaatgctgc
     4801 gacgacacaa aaaccactag tagttctgaa agctattaac agctactatg aacaggacaa
     4861 tgccaatgtt caccgtggtg tccatacctt agcggaacga gcgacagctt cttatgaagc
     4921 tgctcgtgaa accattcgta agtttattaa tgcatgctct acaaaggaag ttctctttac
     4981 cagaggaacg acaaccagcc ttaactgggt ggcacgcttt gctgaggaaa ttctcactga
     5041 gggagaccag gtcttgattt cagtaatgga acaccattct aatatcattc catggcagga
     5101 agcttgtcga aagactggag cagagcttgt ctatgtctat cttaaagacg gtgccttgga
     5161 tatggaggat ttgcgagcta aattgactga taaggttaaa tttgtttccc tagctcatgc
     5221 ctccaatgtt cttggtgtgg tcaatccgat taaggaaatc actcaattag cccaccaagt
     5281 tggggcaatc atggtagtgg atggtgctca atctacacct catatgaaga ttgatgtcca
     5341 ggacttggat ctggactttt tcgccttttc gggtcacaag atggctggtc cgactggtat
     5401 cggtgtcctt tacggcaaag aaaagtatct tgagcaaatg tctccagtag aatttggcgg
     5461 cgagatgatt gattttgtct acgagcaatc cgctagttgg aaggaattgc cttggaaatt
     5521 tgaggctgga acgccaaata tggcaggagc tattggactt gcgactgcag ttgattatct
     5581 ggaaaagatt ggtatggatg ccgttgaagc tcatgaacag gaattgattg cgtacgtcta
     5641 tccaaaactg caggcaattg agggattgac catttacggt tctcaggatt tggctcaacg
     5701 ttcgggtgtt attgccttta acctaggtga tctccatcct cacgatcttg cgacggctct
     5761 ggattatgaa ggagtggctg ttcgtgctgg tcaccattgt gcgcaaccct tgcttcagta
     5821 tttggaagtc ccagcatcag ctcgtgcaag tttttatatc tacaatacca aggcagattg
     5881 cgacaaacta gtcgatgccc tacaaaagac aaaggagttt ttcaatggca ctttctaaac
     5941 tagatagcct ttatatggca gtggtagcag accattcgaa aaatccacat caccaaggga
     6001 agttagaaga tgctgagcaa atcagtctca acaatccgac ttgtggggat gtcatcaacc
     6061 tctctgtcaa gtttgatgca gaggaccgtt tggaagatat tgcttttcta aattcaggat
     6121 gcacgatttc aactgcttct gctagtatga tgacagatgt cgttttagga aaaaccaaac
     6181 aagaaatttt agaactggcg actatttttt ctgaaatggt tcaagggcaa aaagatgagc
     6241 gtcaaggcca acttggagac gcggcattct tgtcaggtgt tgccaaattc cctcaaagaa
     6301 tcaagtgtgc aaccctagct tggaatgccc ttaagaaaac aattgaaaat caagaaaaac
     6361 agtaagacaa gtttcttttg tcttatgaat tattagaaat gaagaaagaa aggatactat
     6421 ggctgaagaa agagtagaac caaaaccaat tgaccttggt gaatataaat ttggtttcca
     6481 tgacgatgta gagcctgtct tatcgacagg aaaaggactc aacgaaggtg ttattcgtga
     6541 attatctgct gctaagggtg agcctgagtg gatgttggag ttccgtttga agtcttatga
     6601 aaccttcaaa aaaatgccca tgcaaacttg gggagcagac ttgtcagaga ttgactttga
     6661 tgacttaatc tactaccaaa aaccatctga caaaccagcc cgttcttggg atgatgtacc
     6721 tgaaaagatt aaagaaacct ttgaacgtat cgggattcca gaagctgaac gtgcttattt
     6781 agcaggggct tctgcccagt acgagtcaga agtggtttac cacaacatga aggaagagtt
     6841 ccaaaaatta ggtattatct ttacagatac agattccgca ctcaaggaat acccagactt
     6901 atttaaacaa tactttgcga agttggtacc gccgacagat aacaagttgg cagccctcaa
     6961 ctcagcagta tggtcgggtg gaacttttat ctacgtgcca aaaggtgtca aggtagatat
     7021 tccacttcaa acttatttcc gtatcaataa cgaaaatata ggtcagttcg aacgtacctt
     7081 gattatcgtt gatgagggag caagcgtcca ctacgtagaa ggatgtacag caccaacata
     7141 ttcaagcaat agcttacacg ctgccattgt agaaattttt gctttggacg gagcttatat
     7201 gcgttataca actatccaaa actggtctga taacgtctat aacttggtaa caaagcgtgc
     7261 taaggctcaa aaggatgcca ctgttgagtg gattgatgga aacttgggtg ccaaaacgac
     7321 tatgaaatat ccatctgttt accttgatgg agaaggagcg cgtggtacca tgctctctat
     7381 cgcctttgct aatgcagggc aacaccaaga cacgggtgct aagatgattc acaatgctcc
     7441 acataccagc tcgtctattg tgtctaaatc catcgctaaa ggtggaggaa aggttgacta
     7501 ccgtggacaa gtcaccttta acaagaactc taagaaatct gtttcccaca ttgaatgtga
     7561 taccattatc atggatgact tgtcagcatc agatactatt ccatttaatg aaattcacaa
     7621 ctcgcaagta gctttggaac acgaagccaa agtatctaag atttcagaag agcaattgta
     7681 ttatctcatg agccgtggat tgtcagaatc tgaggcaact gaaatgattg tcatgggatt
     7741 tgtagaaccc tttacaaaag aacttccaat ggaatacgca gttgagctga accgcttgat
     7801 tagctatgaa atggagggat cagttggata aaatttgatt ttatactctt cgaaaatctc
     7861 ttcaaaccac gtcagcatcg ccttaccgta tgtatggtta ctgacttcgt cagtttcatc
     7921 tacaacctca aaacagtgtt ttgagcaacc tgcggctagc ttcctagtta gttctttgat
     7981 tttgagtatt agatttactc aaaatcaagg attttgaaga tgaacttgta tcaaaaaatc
     8041 gcggtttaaa atcgcgattt tttataattt ctcgttaaca aagcggacaa actgattcca
     8101 ccaaactttt aagaagaagg ctttttcaat tttcttgtct gctaccattt cgaaactagg
     8161 gcgctctgtg gtgatgtaac cttgaccaat caagtccttg tcttcataag tcaaatggcc
     8221 aaccactgtt ccagcttcaa gtggtgctgg gattgctttg gaatcaggtg tgaattgaac
     8281 agattgggaa gattgattcc caacacgttc gattagatag atatcctctg gagccactgc
     8341 agttactgta tcttcttttc catcttgtac aggggctttg ctatcttgat aggcatcgcc
     8401 ttgttgaacg attttgcgaa gtgtaaatgt agaagaaata taatccatta gggaagatgt
     8461 agctgtaaat cgagcgtaag gattattgtc ttgatgatct gcatttaaaa caactgtgat
     8521 gactctcatg cctttttcga cagtagtacc aacaaaagac tctccagcct tatctgttgt
     8581 tcctgttttt agcccatcaa aaccaccacg gtaagcaggc ataccttcta acatgtagtt
     8641 ggttgaagtg attgtcatcc cagcaaaagt agaagaaggt tttttggtga tttctaagac
     8701 ttgtgggtat tttttgatga ggttgcgagc aacgatagcg acatcataag cactaagctt
     8761 attttcctca tcttttttag aacctgggta aatgttatcc cctagagttt cattgttaag
     8821 acctgtcgta ttgacaacag tggcatcctg aattccccat tccaagagtt ttgcccgcat
     8881 catatcgacg aaatcttttt ctgagccagc aattttctca gctagggcaa tagcggcgct
     8941 gttggcacta gataccagag ttgcttcaag caactcttcg acagtataat tacgggcctc
     9001 cataggaata ttactggctt cagaatttgt cgtcaattga taaggataat cagaaatatc
     9061 tacaggagtg gagagggtaa tacttccgtt ttccaaagct tcatagacca gataaacagt
     9121 aatcaatttt gttatggaag caatttcgac aggttgcgtt gcatccttct catagagaat
     9181 tttaccagta tttgcctcaa cagcaatcgc atgtttagcg gcaatggtaa aatcttgagc
     9241 aacagcagta gaagcacccc ctaaaagaga gacagttaac aaagttaaaa atattttttt
     9301 catagtagtc ttattctatc ataaagaaaa aaaatattct tgctttaata attcatctgt
     9361 taagcttttt gaaaatatgg taaaataaag tgagggaggt aactcatgtt tcgtagaaat
     9421 aaattatttt tttggaccac agaaatttta ctcttaacca tcatctttta tctatggaga
     9481 cagatggggt ctttgattaa cccttttgtt agcgtgctta atacaattat gattccattt
     9541 ttattagggg gctttcttta ttatttgaca aaccctattg ttactttctt aaataaagtc
     9601 tgtaaactca atcgtttgct tggtatttta attaccttgt gtactttgat ctggggaatg
     9661 gtcataggtg ttgtctatct cttacctatt ttgattaatc agttatctag tttgattata
     9721 tctagtcaaa ctatttatag tcgagtacaa gacttaatca tagacttatc taattatcct
     9781 gcgctccaga atttggatgt agaagctaca attcagcagt taaacttatc ctatgttgat
     9841 attcttcaaa atatcctaaa tagcgtatca aatagtgtgg ggagcgtctt gtcagctctt
     9901 atcagtactg ttttgatttt gattatgact ccagtttttt tggtttattt cttattagat
     9961 ggacataaat tcttgcccat gcttgaaaga acgattctaa agagggatcg cttgcatatt
    10021 gcaggcttat taaagaattt aaatgcgacg attgctcgct atattagtgg agtttcgatt
    10081 gacgcaatca ttataggttg tttggcttat attggctata gtattattgg tttaaaatat
    10141 gttttagttt ttgccatttt ttctggtgta gccaatttaa ttccttatgt ggggccaagt
    10201 attggtttga ttcctatgat catcgcaaat atattcactg atccccatag actgctgatt
    10261 gcagtgattt atatgcttgt tgttcagcag gtagatggca atatcttata tcctcaaatc
    10321 gtaggaagtg ttatgaaggt tcatccaatc acgattttag ttttactttt gttgtcaagc
    10381 aatatctatg gtgtagttgg aatgattgtc gcagtgccaa cctattctat cttgaaagaa
    10441 atttctaagt tcttatcccg tttgtatgaa aatcataaaa taatgaaaga acgagaaaga
    10501 gaattagcta agtaaaagtc aggagaaccc tgatttttct ttactggaag tggcctttag
    10561 attagaagac tgaaaataag ttaaagtctt aaactaattt tcacagctaa gaatagtaga
    10621 agttaatctg ataaaaatcg aaaaaaccag tggaattccg tgtcagggta agttccactg
    10681 gttttcatag tctattaaag ttcgaatgaa acctatttat agtatattga aactagaata
    10741 gtacacctct a
//
LOCUS       DAVKGU010000065        10749 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKGU010000065 DAVKGU010000000
VERSION     DAVKGU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10749
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.065"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            210..1205
                     /locus_tag="VW077_001531"
     CDS             210..1205
                     /locus_tag="VW077_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9455529.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVRKSSVTKELKEKILHYHNQ
                     KFSPEMMVMVKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGIKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRN"
     gene            1231..1874
                     /locus_tag="VW077_001532"
                     /pseudo
     CDS             1231..1874
                     /locus_tag="VW077_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
     gene            complement(1916..3904)
                     /locus_tag="VW077_001533"
     CDS             complement(1916..3904)
                     /locus_tag="VW077_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9455530.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVIAVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSDIYNQFYGGMNVSISEEEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSVLFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3906..4664)
                     /locus_tag="VW077_001534"
     CDS             complement(3906..4664)
                     /locus_tag="VW077_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455531.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            5092..5283
                     /locus_tag="VW077_001535"
     CDS             5092..5283
                     /locus_tag="VW077_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455532.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(5637..5909)
                     /locus_tag="VW077_001536"
     CDS             complement(5637..5909)
                     /locus_tag="VW077_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9455533.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5968..6417)
                     /locus_tag="VW077_001537"
     CDS             complement(5968..6417)
                     /locus_tag="VW077_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9455534.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            complement(6404..8533)
                     /locus_tag="VW077_001538"
     CDS             complement(6404..8533)
                     /locus_tag="VW077_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9455535.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFVTRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            8577..8660
                     /locus_tag="VW077_001539"
                     /pseudo
     CDS             8577..8660
                     /locus_tag="VW077_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            8990..9895
                     /locus_tag="VW077_001540"
     CDS             8990..9895
                     /locus_tag="VW077_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9455536.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            9892..10707
                     /locus_tag="VW077_001541"
     CDS             9892..10707
                     /locus_tag="VW077_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9455537.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
BASE COUNT         3209 a         2263 c         2081 g         3196 t
ORIGIN      
        1 attagaaata gattttactg tcctgatcga tttgttctgt tcttgtttca ttttactata
       61 tgttcgtaaa tgaaagttct ataatgaagt tagccaccca gccctcctaa aaattagatg
      121 gttcaactag ctgatagctc ttgtaggcat aagaaaacac ctctgtgtta tacttgttgt
      181 tcaaccacaa acacaagaaa ggcacagaga tgcaagacaa ttatactaca aaagccaaac
      241 atttgacaat cgatagccgt cgcttaatcg aaagatggaa aaaagaaggg aaatcaaata
      301 gagaaattgc ctctctgctt ggaaaagttc ctcaaactat ccacactgaa atcaagcgtg
      361 ggacagtccg acaatgtctt ggaaaagggc gcttcaaaga ggtttattct gccgactatg
      421 ctcaacagtc ttatgaaaac aatcgcaagc gctcggtcag gaaatcaagc gtgaccaagg
      481 aactaaagga aaagattctc cactatcata accaaaaatt ttcgcctgaa atgatggtga
      541 tggttaaagg ggttaacgtg ggaatttcaa ccatttacta ttggattcat catggaaaat
      601 tggggttaag caaacaggat ctgctttacc ctagaaaagg aaaagcgctt aagaaacagg
      661 ctagcaccaa ctttaaacct gctggtcaat ccatcgaaca gcggtctgaa gctatcaatc
      721 ttcgcttgga gaatgggcat tatgagattg atacggttct acttacgaga gcgaaaaact
      781 actgtttgct tgtcttgact gatcgaaaga gtagacatca gatcatccga ttgattccaa
      841 ataaaagtgc tgatgtggtc aatcaggctc taaaactcat cttaaaacaa cacaagattc
      901 tttccatcac ggcagataat ggaacggaat tcaatcgctt gtttgatgta ttttctgagg
      961 agcacatcta ttatgcgcac ccctatgcct cttgggaaag gggaactaat gagaatcaca
     1021 acaggctcat tcgtagatgg ttacctaagg gaatcaagaa aatgactccc aaagaagtcg
     1081 cattcatcga aaagtggatt aacaactatc ctaaaaaatg cttggactac aagtcaccca
     1141 gagaagactt ctggatggct aacttgaact tgaaatttag cgtcagaaac aaatcacgaa
     1201 attgattcaa attttgcagg tcgtttaaat atcctgcgtg cgggtgttct tgatgctaac
     1261 gatggaatta tttccattgc tggtgtggtt atcggagttg ccagtgccac gagcaatatc
     1321 tggattatct ttttatcagg ttttgcggct atcttagctg gtgccttttc aatggctggt
     1381 ggagaatatg tatccgtttc aactccaaaa gataccgagg aagctgccgt ttcgcgagaa
     1441 aaactcttgc tagaccaaga tagggaacta gccaaaaaat ccctctatgc tgcttatatc
     1501 caaaatggag aatgcaaaac ttctgcccaa ctcttgacca ataagatctt tcttaaaaat
     1561 ccactcaagg ctctggtaga ggaaaaatat gggattgagt atgaagaatt taccaatcct
     1621 tggcacgctg ccatttctag cttcgttgcc tttttcctta gaagtttgcc tccaatgctg
     1681 tcagtgacca tattcccaag tgaatatcgc atccctgcta ctgtccttat tgtcggtgtg
     1741 gcccttcttc tcactggtta cactagtgcc agacttggaa aggatccgac tagaacagct
     1801 atgattcgga acctagctat ggtctcttga ctatgggagt taccttcctg ctcgaacaac
     1861 ttttcagcat ttagaataca agaaatacct cgattttgaa gtcgaggtat cttttttaca
     1921 tttgcacaat cttacgataa cttcttgaag taatcatgaa aatcagcaca taggcgatga
     1981 ggaagatagc gcagatagac aaggtcacga tcaacatcat agtcgtatcc agtacaccaa
     2041 tcacttttaa aatcaggcta agcatatggt aggcaaaggc tagatgtatg aaggcaaaaa
     2101 gcaaaggaag gaagaaaaca gttaaaacct gtttgttgat ggtttgcttg atttgctttt
     2161 gatctaaacc gactttctgc aagataataa agcgttcacg gtcttcatag ccttcagaaa
     2221 tttgtttgta gtagatgacc agaacggttc cgaccataaa gataatggat aggaaaatac
     2281 caataaagaa gacaccgcca aagaggacac tcatttgagc actagcatct gctagagtgc
     2341 taccatacac atagttacct tcagtgttta attgagcatt aaacttttgt aagtattttt
     2401 catattcttc agcgaccttg agttgctctt cttcactgat acttacattc ataccaccgt
     2461 aaaactgatt atagatatcc gaatctggga attgatccaa aaaggcttgt aaatcaggaa
     2521 caacaaggta attgtaatca gtagtcaaga tattaaactt atttggaaca tggttcacaa
     2581 tgaaatcttt attaaattct tctttgacag aaaattgatg atcatttaga gttagagctt
     2641 tctgtccttt cagtccgtca tttttggcaa agagaccgac ctcatttcct gatagagaca
     2701 gtttttgacc agtcatattt tcataatctt tttggtcaaa taccatgaaa actgttttgg
     2761 gttggacacg gttttgtcct ttttcaaaaa tagttaactt ggttccttct tgatttgcaa
     2821 taccaaagtt actgtaacga agtacttctt tctctttgac actataacct ttgtcacttg
     2881 caaactggct caagagtttg tccaaatctt ctttttcaac attttgccct gaaaccccaa
     2941 aatcatgagg atttagaact tttttaaagc tttctgcgga attgaaaatg cttgtcgctg
     3001 ctgacatggt taccaaaacc attgttgaca aaatagcgat ggttgctagt ccaaccgcat
     3061 ttttcttcat acggaaaatc aagttggaaa cagatatgag gttattaggt tggtaatagt
     3121 atttcttgtt tttctttaag atttgtagga agactgtaat ccctgcatta aacaagagat
     3181 aggtcccaaa gataaccagt aaaacagcta tgaagaaggt tgttaaggct gtaagaggat
     3241 cttttaccgt aagggcaaga taatagccaa tccctaaact tatggaacca agaatagttt
     3301 ggagaggtag gaaacgacct cttttctctc cgcttgcttt ctcacgcgag agctggaggg
     3361 cattcatacg ggcgattcga agggcattca ggaacatgag gcctaggaaa atcaatccaa
     3421 agacaagaag tactgcaatg acaactttca tctggaaggt agcaaccagc tcaaccttca
     3481 atttcattag tttgagcagg aaagcgaaaa ttaacttgtc aaacaaggct ccaataccga
     3541 tacccgctcc aacagttaga atcccaaata ccactaactc cttaaaggtc atactgataa
     3601 gatgacgctt ctccaagccc aacatgccat aaattcctag ttccttggaa cggttcttca
     3661 tgacaaaact attggcatag agaacgataa tggctgacgc aagggtgacg acaaacatac
     3721 caaatccaag tgtagcctga atggttgttc ctccacgaat ttcagcaatc ttgggattga
     3781 aggttagaga gtaaaagaga taggtgacag tgactgccaa gagaacagct agcgcaaaag
     3841 gatagtagag tttgcggttt ttaatcaagt tcgataccgc taacttatta gttaatcgaa
     3901 acatactaat tcacctcgct tgccatgaca gtcaaggtat cagagatttc ttggaacatc
     3961 tgacgttctg tcttctctcc acggtagatt tggttgtaaa gaatgccgtc tttgataaag
     4021 agaacacgct tggccctgct agcagctgct gttgagtggg ttaccatgag gatggtttgg
     4081 ccgcgctcat tgatttcatc aaagacatca agtaaggctg cagatgactt ggagtcaaga
     4141 gctcctgtcg gttcgtccgc aaggagaatt tcaggttcag tgatgatggc gcgtgctact
     4201 gctacacgct gtttctggcc accagaaatc tcgtaagggt acttctcttg taattggttt
     4261 attcccagat tctcagctgt caccaccaat ttcttcatca tctctgtaat aggtcttctt
     4321 gacaagacaa gcggaagcaa gatattgtcc tttacagata gagtatctag caagttaaag
     4381 tcttggaaga caaagcccaa cttttcacga cggaagctag aggcctgtga atttttaatg
     4441 gttgcggtgt cagttccatt caagtaaacc tgaccacgac ttggtttatc cagcatagct
     4501 agaatattga gaagagttga tttaccagaa ccagactcac ccatgatggc aacgtagtca
     4561 cccttttcga cggtaaagtg aatatccttg agggcctcta cttggttgcc ctgaaaacga
     4621 gttttataaa ttttttgaac gtgttttaca tctaaaagtg tcatgagaat ctcctttctt
     4681 aatatcccat caaatgaaat gtcgcttgca tcatagcgca tcccagctga acgcgctcga
     4741 tttctttgtg acttggtttt cttgttttct tatgtaagat ttgtttcatg atgttactcc
     4801 tttgttcttt atgagtctag tttacatcaa aaaaagacct cttgccataa cctaacctta
     4861 caaaagagac tttaatctta catttttgta agattggggg aaatgtaagc aaaattactt
     4921 agaaaatgat ttgatttcct atttttaaaa ttgtaaaata taaatatgaa aaggcttact
     4981 ttggaaagga ggaagcggga actaacattt tagtaagctt acctagataa cataccattc
     5041 aatttaacac ttgcaagtcg ttcaacctat aaggaaagaa ggtgcgcctt aatgaaacac
     5101 aaagaacata tactcatcgg acttctctat ctgctctctc cattcatcgg tcagctctta
     5161 gttgaacaca cacactttat cagtacagaa tttaccggca ctgcttatgt tatttgctgg
     5221 ttatcagttg ttatcagcat ccaccatttt tccaaaaatg tattgtctca acagcaaaaa
     5281 tagattaaaa aactgcgttt aaattactgc ttttatagta aaatgaaata agaacaggac
     5341 aaatcgatca ggacagtcaa atcgatttct aacaattttt tagaagtata ggtgtcctat
     5401 tctagttcca atccactata gtttgtggat gaagctggcg aaatattatt aatactattc
     5461 aaaaatcaaa gtgcaaacta tatcaacttc acattgccga ctaaattaca gcctgcggct
     5521 ggcttcctag tttgcacttt gatttttatt gagtataaaa gtaaaaaagc tttaaatcaa
     5581 gcagtttaga ctattgattt aaagcttttt gcgtattcca aatcttttgt aaaacctcac
     5641 caaaaccagc cttcattatc gtcaatggct tgagcttctt tgcgtttacg tgggcctgtt
     5701 ccgtacattt ctgcttcgat ttcttccttg gttggcatga tagaagctag gatgatatag
     5761 ataatcaccc caagtccaaa gtttgcgact gtaaaaatgg caaacagaaa acgtacaagg
     5821 gtaacatcaa agttccactt gtctgacaga cctgctaaaa ctcctgaaat catgcgattt
     5881 cgtctcattt tataaaactt actgttcatg atatttcctc tttctgctag gttagacata
     5941 gtatatcacg gataggctcg gctttcatca gtcctcaggc tgatttacta atagcaactt
     6001 tcctcgacaa agtccacagc gatagcgttt ggtatcaatc ctacgcttac gctgataagt
     6061 ttgctggcag gattggcaac gatagagctt gattggcttg gagttactat tgggcaagga
     6121 tggtacaaac cgtaatccat ccactgcttt caacagttcc ttaaaatccc gatccttgtg
     6181 ttgatagcct ttcccttgaa aatagaggtg ataatgacag agttcatgtc ggacaatttt
     6241 cctaaaaacg tccaacccca gttcctgata aaccttggga ttaaaatcca aatgcccatc
     6301 tttggggaaa aatcgcccac ctgtcgaacg tagacgccta ttccactgga catgatggat
     6361 aaaaggtctg ccgaagtctt ctagtgaaac ctgcttgacg taatcagtca gtttcatttg
     6421 gagctaggag agacagatta actttttcac gttcagtatc aattttctta acccaaacgc
     6481 tcaccaaatc tccaactgcc accacttgac tagggtgttt gataaacttg cgactcatat
     6541 gggaaatatg gatgagaccg tcctcatgaa ttccgatatc aacaaaagca ccgaaatcaa
     6601 caacgttacg caccactcct tctagctttt gtccaaccac taagtccttg atatctagga
     6661 catcttgacg gagaactgga gcatcaaagg aatcacggaa atctcgacct ggtttgagaa
     6721 gatctgcaat gatatcttta agagtttctg gaccaaggtc taactcttgc gccatttcct
     6781 tgactgaaag cgacttgagt ttgctttggg cctcttcatt cagatcctta atgtccaagc
     6841 atttgaagag ttccttaacg gcagcgtaat tttctgggtg aactcctgta ttatcaagga
     6901 tattgctact ttcagggata cggaggaaac cagcagcctg ttcaaaagcc ttggccccca
     6961 aacgaggaac tttcttgatt tgggagcgtg aagtgatttt tccctcttcc tcacggtatt
     7021 tgacgatatt ttcagaaatg gttttattga gtccagctac gtgtgaaaga agagctgggc
     7081 tagctgtatt gacattgaca ccaacttggt taaccactgt atcgacaaca aagtccagac
     7141 tctcagatag tttcttctga ctgacatcgt gttggtattg accgacacca attgacttag
     7201 gatcgatttt gaccaattcc gcaagaggat cttgcaaacg acgggcgata gaaatggcag
     7261 agcgtttttc aacggtcaag tctggaaact cctgacgagc aagttcgctg gcagaataga
     7321 cagaagcacc actttcatta acgataacat agctgacttc agggaaatct ttcagaactt
     7381 ccgctacaaa agcttcactt tcacgactgg ccgttccatt tccaatggca ataatctcta
     7441 caccgtattg accaattaaa tctgctaaat ctttcttggc ttcttcgatt tgacgagctg
     7501 atgctggttt aacaggataa ataacctgag ttgtcagcat ttttcctgtt gcatccacga
     7561 cagctagctt ggcacctgta cgaaaggctg ggtcaaaccc aagaaccacg cgccctttca
     7621 gtggagcaac caagaggaga ttgcgcagat tgtcagaaaa aagttggata gctccctctt
     7681 cagctttctc agttaattct gtccgaatac gacgctcaat agcaggcaag acctttttct
     7741 taacggattg ctgaacaact tcatcaatat aagcattttt caccttgaaa cgagtaacaa
     7801 agaaggcaag aatacggtcc gtcgcatgtt caaaaccgat cttcaagaca ccaagtttct
     7861 ccccacgatt gagagccaag gtacgatagc cttgcatagt tccaactgtc tctgaaaaat
     7921 cataataaat ctgaaaaacc tgcttttcat caagactttc atccttggct tgagaagtga
     7981 gtttagagtg tctcagcact tcctgataag tcatagaacg caaggtcaca tcttccgata
     8041 aggcttcgac caaaatatca actgcaccgg tcaaggcttc cttgccagtc gcaaatcctt
     8101 cacagacgaa cttttcagct tctttctcta agtcaactat attctgcaaa atcaagcgag
     8161 caagaggaaa gagtccagct tcacgggcaa tggttgcctt ggtacgacgc ttttccttat
     8221 aaggaagata gagttcttca acgtctgcta atttttcggc aactaagata gcttcttcca
     8281 attccttggt caacttgcct tgctcttgaa tcttagctaa gacagcttcc ttacggtcat
     8341 tgagatttgt cagactttta tccaaatcaa taatagcctt aatcgccacc tcatccagac
     8401 taccagtcat gtccttacga taacgcgcga taaagggaat agtcgcccct tcagctgtca
     8461 aacttagaac ggtatcaatt tgctttaacg tcactcccaa atcctgagag attttttcat
     8521 attttttatc cataaatcta ttataccaca agctggaagg ctttctccaa tccttttcat
     8581 actcaatgaa aatcaaaaag caaactagga agctagccgc aggctgctca aaatactgtt
     8641 ttgaggttgt ggataagact gacgaagtca gctcaaaaca ctgttttgag gttgtggata
     8701 gaactgacga agtcagtaac cgtactacgg taaggcgaag ctgacgtggt ttgaagagat
     8761 tttcgaagtg tatcaactat cgaaaacaaa ttaaaaaact cccttttccg caaaataata
     8821 gtataataga ggtagaattt agaatcgagg tacacctatg gctgtcaaat ttacaaaacg
     8881 agacgacttg gacaagatgt ttgaagagtt tgctaaactc cctgatttga aacaagttac
     8941 tttccctgat gacaaagaga aaaaagtcaa agcagaaaag aaaaactaga tgactgcttt
     9001 tcaacaactc ccatctagtg tacttcaaac tggagccatt tttctctcca ttatcattga
     9061 agcccttccc ttcgttctga taggaagcat tgtctcaggg ctgattgaag tttatatcac
     9121 acctgacaag gtttatcatt ttctccctcg aaatcgttgg gggagaatct tttttgggac
     9181 ctttgtcggt atacttttcc cttcttgtga atgtggaatc gtccccatca tcaatcgttt
     9241 tctggaaaaa aaggttccaa gttacacggc cgttcctttt cttgtgacag cacctgttat
     9301 caatcccatt gttctttttg cgacctattc tgcctttggc aactccttcc atgtcgccct
     9361 attacgagct ctgggttcca ttcttgtggc tgtaatacta ggaatttttc taggattttt
     9421 ctggcaagaa ccgattcaga aagaaaatcg tctggcttgt catgagcatg atttttctta
     9481 cttgagttct gcaaaaaaag tttttcaagt ctttgtgcag gccattgatg aattttttga
     9541 tacggggcgt tatttggtat ttggctgcct ctttgcttct ataatacagg tctacgttcc
     9601 gactcggatt ctgacctcta tcagtgcgac tcctcttttt gccatcctgc tcttgatgct
     9661 tttggccttt cttctttcgc tctgtagtga ggcggatgcc tttataggtg cttctctact
     9721 ctcgagtttc ggtttggctc cagttctggc ctttctcgtc attggcccaa tgctggatat
     9781 caaaaatatt ctcatgatga aaaattactt gaaagcacga tttattagtc acttcattac
     9841 gattgtgact ctggtcgttt tagcctattc tctcttgatt ggagtcatcc tatgattcga
     9901 tttttagttt tagctggcta ttttgaactg actatttacc tccatctgtc gggcaaacta
     9961 aaccagtaca tcaacatgca ctattcctat ctggcctata tttccatggt gctttccttt
    10021 atcttggcaa ttgttcaatt gtatatctgg atgaagcaag tcaaaaccca cagtcatctg
    10081 aacagccgat tggccaagat gacgagtatt tctcttctgg ctattccact tgtcatcggc
    10141 ttaactttcc caactgttag cttggattct cagactgttt ctgctaaagg ttatcatttc
    10201 cccctatcgg aaggaacgga tctagccatt cagacaagcg aagggacgac aagccaatat
    10261 ttgaaaccag ataccagttc ttatttttca aaatcagcct atgaaaagga aatgcgaacg
    10321 gcggcggata aatacttatc ccaagatagt attcagatca ctaatgaaaa ctatatggaa
    10381 gtcatggagg ctatctacga ctatccagat gagtttgagg gcaagacaat ccagtttaca
    10441 ggctttgtct ataacgaccc cagtcatgcc aatagtcaat ttctgttccg attcggcatt
    10501 atccactgta tcgcagattc tggtgtctat ggattgctga ccaagggcaa tacccggcag
    10561 tatgaaaaca acacttggat ttcagccaaa ggaaaactgg tcaatcacta ccataaagaa
    10621 ctcaaacaaa accttccaac cttggaaatc gacagcttta ccaaagtcga taaaccagaa
    10681 aatccctatg tatatagagc tttttaagaa aatcaaaata aaaacgaaca agttctcttc
    10741 tgaataaca
//
LOCUS       DAVKGU010000066        10713 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKGU010000066 DAVKGU010000000
VERSION     DAVKGU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.066"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            89..891
                     /locus_tag="VW077_001542"
                     /pseudo
     CDS             89..891
                     /locus_tag="VW077_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            940..1404
                     /locus_tag="VW077_001543"
     CDS             940..1404
                     /locus_tag="VW077_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9455538.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            1561..1986
                     /gene="lacA"
                     /locus_tag="VW077_001544"
     CDS             1561..1986
                     /gene="lacA"
                     /locus_tag="VW077_001544"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9455539.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2017..2532
                     /gene="lacB"
                     /locus_tag="VW077_001545"
     CDS             2017..2532
                     /gene="lacB"
                     /locus_tag="VW077_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9455540.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            2543..3472
                     /locus_tag="VW077_001546"
     CDS             2543..3472
                     /locus_tag="VW077_001546"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9455541.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIKGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQEFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            3474..4454
                     /gene="lacD"
                     /locus_tag="VW077_001547"
     CDS             3474..4454
                     /gene="lacD"
                     /locus_tag="VW077_001547"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9455542.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            4688..5524
                     /locus_tag="VW077_001548"
     CDS             4688..5524
                     /locus_tag="VW077_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9455543.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLITENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            5560..5877
                     /locus_tag="VW077_001549"
     CDS             5560..5877
                     /locus_tag="VW077_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9455544.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5879..7573
                     /locus_tag="VW077_001550"
     CDS             5879..7573
                     /locus_tag="VW077_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9455545.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            7788..9194
                     /gene="lacG"
                     /locus_tag="VW077_001551"
     CDS             7788..9194
                     /gene="lacG"
                     /locus_tag="VW077_001551"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9455546.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGHYSEKTLAGVEAILAANGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDVIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            9237..9494
                     /locus_tag="VW077_001552"
     CDS             9237..9494
                     /locus_tag="VW077_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9455547.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDELEEFKNLFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(9772..10533)
                     /locus_tag="VW077_001553"
     CDS             complement(9772..10533)
                     /locus_tag="VW077_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9455548.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
BASE COUNT         3441 a         1829 c         2165 g         3278 t
ORIGIN      
        1 ccaatcttat tccgtatccc agcaatgagc gttccagtat ttgacaatat tagacttcct
       61 gcgaaacaaa acatggtata gtagttctat gaatgatgaa gcaagtaaac aactaactga
      121 tgcacgattt aagcgtcttg ttggtgttca gcgcacgact tttgaagaga tgttagctgt
      181 attaaaaaca gcttatcaac ttaaacacgc aaaaggtgga cgaaaaccta aattaagcct
      241 agaagacctt cttatggcca ctcttcaata tgtgcgagaa tatcgaactt atgaagaaat
      301 tgcggctgat tttggtatcc acgaaagcaa cttactccgt cggagctaat gggttgaagt
      361 aactcttgtt caaagtggtt ttacgatttc aagaactcct ctcagttctg aggacacggt
      421 aatgattgat gcgacggaag tacaaatcaa tcgccctaaa aaagaattag cgaatgattc
      481 tggtaaaaag aaatgccacg ctatgaaggc tcaagcgatt gtcacaagtc aagggagatt
      541 gtttctttgg atatcactgt gaactattgt catgatatga agttgttcaa aatgagtcgc
      601 agaaatatcg gacaagctgg taaaatcttg gctgacagtg gttatcaagg gctcatgaag
      661 atatatcctc aagtacaaac tccacgtaaa tccagcaaac tcaagccgct aacagttgaa
      721 gataaaacct ataaccatgc gctatctaag gagagaagca aggttgagaa catctttgcc
      781 aaagtaaaaa cgtttaaaat gatttcaaca acctatcgaa atcatcgtaa acgcttcgga
      841 ttacgaatga atttgattgc tggcattatc aatcatgaac taggattcta gttttgcagg
      901 aagtctaata aagaaactag caaggtcagg ccttacttta ttggtggaca tccaggattt
      961 aattgtcctc tacttgatga tgaagtctat gaagattatt atctagagtt tgagaaagaa
     1021 gagacttgct ctgttccacg tcctttccca gaaacaggta tgttggattt ccaagataga
     1081 agtccatggc tagagggaca aaaagaaata gatcttagtt atgatctttt cagcaaagat
     1141 gcagtgactt tggatgaatt gcaatctcga acaattgccc ttcgttctct taaacatgat
     1201 aggggattga aagtgcattt tgcagaattt ccaaacctca tcatctggtc aactttgaac
     1261 aagggacctt tcattacctt tgaaccatgg tctggcttgt caacattcct tgaagaagga
     1321 gatcatttag aagataagaa gaatgtttgt ctcttagaag ccaatcaggt tgaagaatta
     1381 gggtttgaga tagaagtttt ataaaaaagt aaaaaataaa taacaaaaac aaacacaaac
     1441 tgttgacttc ttcaaacaat agtagtatat tatgtttgtg aggactaatt gactgttttt
     1501 taaagacagc caatacactg ctggaatcca gcatagaaaa aataaaaagg agtatacaat
     1561 atgtctattg ttatcggtgc agatgctgca ggtttgagat tgaaagaagt tgtgaaagac
     1621 ttcttagaaa aagaaaactt ccaccttgtg gatgttacag ctgaaggtca agactttgtt
     1681 gatgtgactc tcgctgttgc tgcagaagta aacaaagaag aacaaaacct tggtatcgtg
     1741 attgatgctt atggagctgg tccatttatg gttgcaacta agatcaaagg aatggttgct
     1801 gcagaagttt ctgatgaacg ttcagcttat atgactcgtg gccacaacaa ctcacgtatg
     1861 atcactatgg gtgcacaact tgttggtgat gaattggcta aaaacatcgc taaaggattt
     1921 gttaatggta aatacgacgg tggtcgtcac caaatccggg ttgacatgtt gaacaaaatg
     1981 ggctaattag attatagtaa gaaaggtaag ttaaaaatga gaattgcaat tggatgtgac
     2041 cacatcgtaa cagatgaaaa aatggcggtt tcagaatttt tgaaatcaaa aggatatgaa
     2101 gtcattgact ttggtacata tgaccataca cggactcact acccaatctt tggtaaaaaa
     2161 gtaggggaag ctgtaactag cggtcaagct gatcttggag tatgtatctg tggtactggt
     2221 gttggtatca acaacgctgt aaataaagtt ccaggtgttc gttctgcctt ggttcgtgat
     2281 atgacaacag ccctttatgc taaagaacaa ttgaacgcta acgttattgg ttttggtggt
     2341 aaaattactg gtgaattgct tatgtgtgat atcatcgaag ctttcatcca tgctgaatac
     2401 aaaccaactg aagaaaacaa aaaattgatt gcgaaaattg aacatgttga aagtcacaat
     2461 gctcaacaaa cagacgcaaa cttctttaca gaattccttg agaaatggga tcgtggagaa
     2521 taccacgact aagaggtgac ctatgatttt aacagtcaca atgaacccat ccatcgatat
     2581 ttcctatccc ttggatgagt tgaagattga tactgtcaat cgtgtggtgg atgtaaccaa
     2641 aacggctggt ggtaagggac tcaatgttac ccgagtactt tcagaatttg gcgattctgt
     2701 tcttgctact ggtttagtgg gtggcaaact tggtgagttt ttggttgaac atatcgataa
     2761 tcaagtaaag aaagatttct tctcaattaa gggagaaact cgtaactgta tcgctattct
     2821 ccacggagac aaccaaacag aagttcttga aaaaggtcct gaagtattgg aacaggaagg
     2881 tcaagagttt ttggaacatt tcaaaaaact cttggagtca gttgaagtag tggccatctc
     2941 aggtagtctg ccagctggcc ttccagttga ttactatgcg agcttggtag aacttgctaa
     3001 tcaagctggc aagcctgtag tcttggactg ctcaggtgca gcacttcagg ctgttcttga
     3061 atcaccccat aaaccaacag tcatcaaacc aaataatgaa gaattgtctc agcttcttgg
     3121 aagagaagtt tctgaggatt tggatgaatt aaaagaagta cttcaagaac ctttgtttgc
     3181 agggattgaa tggattatcg tttcacttgg tgccaacggt acttttgcca aacatggtga
     3241 cactttctac aaggtagata ttcctagaat tcaggtggta aatcctgttg gatctggaga
     3301 ctctactgtg gcaggaattt cttcaggact tcttcacaaa gaatcggatg cagaattact
     3361 catcaaggca aatgtccttg gtatgctcaa tgctcaagaa aaaatgactg gtcatgtcaa
     3421 catggccaac tatcaagttc tatatgatca attaatagta aaagaggtat aaaatggctt
     3481 taacagaaca aaaacgtgta cgcttagaaa aactttctga tgaaaatggt atcatctcag
     3541 ctcttgcatt tgaccaacgt ggtgctttga aacgcctcat ggctcaacac caaacagaag
     3601 aaccaactgt ggcccaaatg gaagaactta aagtcttggt agcagatgaa ttgactaaat
     3661 acgcttcatc tatgcttctt gaccctgagt atggacttcc agcaactaaa gctcttgatg
     3721 aaaaagctgg ccttctcctt gcttatgaaa aaacaggtta tgacacaaca agcacaaaac
     3781 gcttgccaga ctgcttggat gtctggtctg caaaacgtat taaagaagaa ggtgcagatg
     3841 cagttaaatt cttgctttac tatgatgtag atagctcaga cgaactcaat caagaaaaac
     3901 aagcctacat cgaacgcatc ggttctgagt gtgtggctga agatatccca ttcttccttg
     3961 aaattcttgc ttacgatgaa aaaattgcgg atgcaggttc tgtagaatac gcgaaagtaa
     4021 aaccacacaa agttatcggc gctatgaaag tcttttcaga cccacgcttt aacattgatg
     4081 tcttgaaagt tgaagttcct gttaacatta aatatgttga aggcttcgct gaaggtgaag
     4141 tagtttatac acgtgaagaa gcagcagcct tcttcaaagc gcaagatgaa gcaacgaact
     4201 tgccatacat ctacttgagt gctggtgtat cagctaaact cttccaagat actcttgtat
     4261 ttgctcatga atcaggtgca aactttaacg gagttctttg tggccgtgct acatgggcag
     4321 gatcagttga agcttacatc aaagatggtg aagcagcagc tcgcgaatgg cttcgcacaa
     4381 ctggatttga aaacattgat gagctcaata aagttcttca aacaacagcg acttcatgga
     4441 aagaacgtgt gtaagaaagt cctcctagtt taggaacatg aatctaaaaa aatttaaaaa
     4501 aagttgtatg taaaggttta caaaataact tacttgtgct atacttaaat cacaagttaa
     4561 tacaaggtga gtgttactaa gtaatattag gcatgatcac aggtgaatta gaaatcagct
     4621 gattttctag ttcatttgtg gtcatttttt gtactcatat acctttaaga tataaaagga
     4681 ggttgacatg tatcgaattc taaatccaat gaatcacaat gtctcgcttg tcagaaatga
     4741 taagggagaa gaggtgattg taattggtaa ggggattgca ttcggaaaga agaaggggga
     4801 tttgattact gaaaatcagg ttgagaaaat ctttcggatg aagaccgaag agtccagaga
     4861 aaactttatg gctcttctca aagatgttcc gcttgatttt atcacagtga cctatgaaat
     4921 cattgataag ctatcaaaga aatatcatta tccgattcaa gagtatctct atgtaacctt
     4981 gacagatcat atttactgtt cttatcaagc tctaactcaa ggaaggtaca aggatagtaa
     5041 tctgccagat atttccgcta agtatcctgt cgcttttcaa atcgcaaatg aagcttttga
     5101 aatttaccgt cagaagctag cagatcattt tcctgaggac gaaattattc ggattgctta
     5161 tcatttcatt aatgctgaag gtgaaaatga agtggaactt gtggagtcga ttgataagag
     5221 gaaagaaatt ctcaggaatg ttgaagaagt tttaacggac tatgcaattc aacgaactaa
     5281 aaagaataac catttctatg atcgctttat gatccatttg aattatttct tggattattt
     5341 agacagatct agagatgata accaatcact tctggatatg gaagatcata ttaaacaatc
     5401 ctatccaaaa gccttcgaga ttggttccaa gatctatgat gtgattacgc aacatacggg
     5461 tcttgatttg tataaaagtg aacgagttta tctagttcta catatccaac gtttattgtc
     5521 ataaaaattt atttaaaact atataaggag aattctatca tgaatagaga agaagtaaca
     5581 ttgttaggtt ttgaaatcgt agcctatgct ggcgatgctc gttcaaaact attggaagcc
     5641 ttgaaggctg ctgaagctgg tgattttgaa aaagcggacg ctctggtaga ggaagctggt
     5701 agctgtattg cagaggctca ccacgcgcaa acaagtctat tgactaagga agcttctggt
     5761 gaggacttag cgtatagtgt gaccatgatg catggtcaag atcacttgat gacaactatc
     5821 ttgttaaaag acctaatgca ccatttaatt gaactttaca agagaggagt taaataaaat
     5881 ggataagtta ataattttta ttgagaaagg aaagcctttc tttgaaaaat tatcgagaaa
     5941 tatatactta agagcgataa aagatggttt tatttccagt atgccagcag tattattctc
     6001 aagtattttt atactaatag ctgctgtgcc caatattttt gggtttaaat ggtctgatga
     6061 acagttagct tttattttga aaccttacaa ctactcaatg ggaattttag ctctcttagt
     6121 agctgggaca actgcaaaat cgttaacaga ttcagttaat acacgtagta tggagaaaac
     6181 caaccaaatc aactacatgt caactttttt agcagctgtt gttggattgt taatactggc
     6241 agctgatcca attgaaggtg gatttgctaa tggtctttta ggtactagag gattacttac
     6301 tgcatttttg gcagctttta ttacagtaaa tatctataaa gtatgtatta aaaataatgt
     6361 aaccattaga cttcctgaag aggttccacc aaatattgca caagttttta aagatgttat
     6421 tccgtttgcc ttatctgttc tttcaatata tggattggat ttaattgtta gaaatatttt
     6481 tggtacaaat gtagcagaat ctgttggtaa aatattagcc cctctatttt cagctactga
     6541 tggttatatc ggtttagcta ttgtatttgg tgcttatgct ttcttttggt ttgttggaat
     6601 tcatgggcca tctgttgtag aacctttaat tgtagctatc agttatgcaa atatagaagc
     6661 taatgttcag cttgtgcaag ctggtatgca cgcagataaa attttaaatc cagttactca
     6721 aacttttgtt gtaacaatgg gtggtactgg tgcaacacta gtagttccat ttatgtttat
     6781 gtggttatgt aaatcaaaaa gaaatagaat tgtcggacgt gcgtctgtag ttcctacttt
     6841 ctttggcgta aatgaaccaa tcctatttgg tgctccaatt gttttaaatc ctatcttctt
     6901 tattccgttt gttactgctc ctattatcaa tgtttggatt atgaaatttt ttgtagatgt
     6961 tctacaaatg aatagtttca gtattatttt accttggaca acacctgctc caattggtat
     7021 tgttatggga acggcgctgg ctccattgtc attcgtcttg gctataactt taatcattat
     7081 cgacacttta atctattatc catttgttaa agtttatgac catcaaattt tagaagaaga
     7141 acggaaagga aactcttcat ctgaattgaa agaaaaagtt gctgcaaact tcaacactgc
     7201 aaaagcggat gctattcttg aaaaagcggg tgtcgatgca gcacaaaata ccatcactga
     7261 agaaacaaat gtcctcgttc tctgtgctgg tggaggtaca agtggactcc ttgcaaatgc
     7321 tttgaataag gcagcagcag aatacaatgt ccctgtgaaa gcagcagcag gcggctatgg
     7381 tgctcaccgt gaaatgttac cagagtttaa tctagttatc cttgcccctc aagttgcttc
     7441 aaactttgaa gatatgaagg cagaaacaga taagctcggt attaaacttg ctaagacaga
     7501 aggcgctcaa tacatcaaat taactcgtga tggaaaaggt gccctagcat tcgtacaaga
     7561 gcaattcgat taaggctagg aactgtgaaa cagtctccca tcgttacgga aatcgctatg
     7621 gcgaatttcc taatcgcctt attaattcgt cggtaaaaag atatcgtttt tacctcctca
     7681 tgtcacaatt cagtgacttg gtacaagaag tgagatggag aagcatggct cactgactcc
     7741 tctcctctca cttttacttt atttaaatca agaaataggt gaaaaaaatg acaaaaacac
     7801 ttccaaaaga ctttattttc ggtggcgcaa cagctgctta tcaagcagaa ggtgctacac
     7861 atactgatgg aaaaggacca gttgcttggg ataaatatct tgaggataac tactggtaca
     7921 ctgccgaacc agctagtgat ttttacaatc gatatccagt tgacctcaag ctagcagaag
     7981 agtatggtgt caatggtatt cgaatttcta ttgcttggtc acgtattttc ccgactggtt
     8041 acggccaagt aaatgctaaa ggtgttgagt tttatcataa tttatttgca gagtgtcaca
     8101 aacgtcatgt tgagcctttt gtaactcttc atcactttga cacgccagaa gctctccact
     8161 caaatggaga cttcttaaac cgtgaaaata tcgaacattt tgtagactac gctgctttct
     8221 gttttgaaga atttccagaa gtaaactatt ggacaacctt taatgaaatt ggaccaatcg
     8281 gtgatggtca atatttggtt gggaaattcc ctccaggtat ccagtacgac cttgccaaag
     8341 tctttcaatc acaccacaat atgatggtgt ctcatgcacg cgcggtaaaa ttgtacaaag
     8401 agaaaggcta taaaggggaa attggtgttg ttcacgccct gcctactaaa tatcctctag
     8461 atcatgaaaa tccagcagat gttcgtgcag ctgagttgga agatattatt cacaacaaat
     8521 ttattttgga tgcaacctat ctgggtcact actctgaaaa gactctggca ggggtagagg
     8581 ctatcttggc tgccaatggt ggtagcttgg atcttcgtga agaagatttt acagcattag
     8641 aagctgcaaa agacttgaat gatttcctag gaatcaacta ctatatgagt gactggatgg
     8701 aagcctttga tggagaaact gaaattatcc ataatggtaa aggtgaaaaa ggaagctcta
     8761 agtatcaaat caaaggtgtt ggtcgtcgtg tagctcctga ctatgtacca cgcacggatt
     8821 gggattggat tatctaccct caaggtttgt atgaccaaat catgcgcgtg aagaaagatt
     8881 atcctaacta caagaagatt tacatcactg aaaatggtct cggctataaa gatgagttcg
     8941 ttgataacac tgtttacgat gatggtcgta ttgattacgt gaaacaacac ttggagatct
     9001 tgtctgatgt gattgcagat ggagctaatg taaaaggtta cttcatttgg tcattaatgg
     9061 atgtcttctc atggtcaaac ggttatgaga aacgttatgg tctcttctac gtagattttg
     9121 aaactcaaga acgttatcct aagaaatcag ctcactggta caagaaagta gcggaaactc
     9181 agattataga ctagtagaat tagtcattag atatagaatt ttagtgagtc aaaaagatgt
     9241 tcaaagattt tatccaatct atttatgaaa aagtttatat tataaatttc gaaaaatgct
     9301 ctcaaatacc gtgtttgacg agtgaagaat tgaaaagtct tggaaaatgg tatgtctcga
     9361 ctggtaaaga atggatttgt cattcagatg atgagctgga agaatttaaa aatctatttt
     9421 taaattttat caatcctgaa gaatgggata ctatctcctt tgattcagat tttatgccgt
     9481 ttcaacaatc gtaaccaatt tctcaaaaaa gttaaatctt atatttagta ctctgtaaaa
     9541 ctcttatcta atcacgttgc ttatactcaa tgaaaatcaa agagcaactt taaactagga
     9601 agcgagtcgc agatttctca atgcatagct ttgaggaatt gggcaaaaag tctttgatat
     9661 agaaaaacgc atagtatcag gtgtttcaac acctgatact atgcgtttta ttgtgggaag
     9721 atttactttt ttcttctgaa attgagttgt tacccaggct ctttcagttt attaaggctt
     9781 gatgacttta atgtgtttag atagcttaaa aaggattgaa tcacttagtt tagaatctga
     9841 aacaatagta tcaagatttg atacattata aaaagtataa aaatcaaact tattgaactt
     9901 gctatgatct gcgagtaaat attttttatt agaattattt aaagcgatgc gttgagcctc
     9961 tccctcttcc tcgctaaaag tagctagagc tccgttttga ataccattac agctaacgaa
    10021 agctttagaa aattggagat tagagagatt ttgtagggtc aatgtaccaa caaaagcacc
    10081 tgtaatatcg cgataatttc cacctattaa aatcaaatct gttaattttc gttcgcttaa
    10141 aatcagaaaa acaggtagac tgttggttac gacgcggata ttgtcaatag gcaactcacg
    10201 cgcaaaaaac tctaatgttg ttcctggtcc aatgaaaata gtttctcttt cttctactag
    10261 actgcctgca aaatgggcta tttcttgttt ttctgccgtt tggagggctt gtttttcaat
    10321 atttgatcgc tcattagtca aaagggagtt ggttcgaagt ttttcagctc caccatgcac
    10381 acgaatcagc aaatctttat cagctaattc ctgtaaatag cgccttgcag tcatatctga
    10441 aacggctatt tcgtccataa tctgtttaac tgttatggtt cctttactat ttactatctc
    10501 taaaattttg gctaattttt cttgtttgag catattatca cctcgtttcc tactactatc
    10561 ttaccataaa caaactcatc attcaaatac aaaaacaaca aaataaaaca aaaacaaaaa
    10621 tatcgaggtt tattttcaaa actttcgata tttttattaa gttattattt tgttgtttct
    10681 agtttacttt ttgatggtta agagtggtgg aga
//
LOCUS       DAVKGU010000067        10630 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKGU010000067 DAVKGU010000000
VERSION     DAVKGU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.067"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(222..314)
                     /locus_tag="VW077_001554"
     CDS             complement(222..314)
                     /locus_tag="VW077_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9455549.1"
                     /translation="MMELILKTIIGPIVVGVVLRLADKWLNKDK"
     gene            complement(537..626)
                     /locus_tag="VW077_001555"
     CDS             complement(537..626)
                     /locus_tag="VW077_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I addiction module toxin, Fst family"
                     /protein_id="HEU9455550.1"
                     /translation="MVDYIFTNIILPLVVGIVLIVIEKWLNKK"
     gene            complement(850..1089)
                     /locus_tag="VW077_001556"
     CDS             complement(850..1089)
                     /locus_tag="VW077_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455551.1"
                     /translation="MTFKEYLLKASKRDIYDDGKDFDFETIFAREILRYAHDSELETK
                     TGFFRHLEIMNADSWFVELARSIYQDFEKSISDSH"
     gene            1154..1345
                     /locus_tag="VW077_001557"
     CDS             1154..1345
                     /locus_tag="VW077_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9455552.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            complement(1528..1719)
                     /locus_tag="VW077_001558"
     CDS             complement(1528..1719)
                     /locus_tag="VW077_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455553.1"
                     /translation="MKSFKDFRESLTAEDMQAISAKANEATKQIDHTDGLQLGKVSGL
                     TSVITTVELLEKYHEWLHS"
     gene            1838..1981
                     /locus_tag="VW077_001559"
     CDS             1838..1981
                     /locus_tag="VW077_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455554.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     RTV"
     gene            2060..2332
                     /locus_tag="VW077_001560"
     CDS             2060..2332
                     /locus_tag="VW077_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455555.1"
                     /translation="MEVVEIVRIKDVIIEKVSANDEELKRIFGCSKRQAGERRREMQK
                     LPSQQKHLLDSGQLVTIKGFYEYLQYRGTKAWKKEMETSKKMRSAG"
     gene            2329..2523
                     /locus_tag="VW077_001561"
     CDS             2329..2523
                     /locus_tag="VW077_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455556.1"
                     /translation="MNLLSRIKNYFSEEVKETNLDWKEVALDLNQSLIETQEKLQEAN
                     QEIADLKKIVAIYKEKEKEK"
     gene            2520..2711
                     /locus_tag="VW077_001562"
     CDS             2520..2711
                     /locus_tag="VW077_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455557.1"
                     /translation="MMEYIYLVIIVGIGLWSLVNKLDDHAEMKQKERQLMANNVARMN
                     LRNSDKQFTYDVEPPEGLK"
     gene            2723..3064
                     /locus_tag="VW077_001563"
     CDS             2723..3064
                     /locus_tag="VW077_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455558.1"
                     /translation="MTQAEQIREYYREHPAASCDEVAEVVGTTKSNVSANLAKDIKAG
                     RCVRLEDKSYDYSPYFNHTQALTELVDWKNDTRREWVDMLTRAAEKETDSNVMRLLIK
                     EANKLMKEVTK"
     gene            3065..3295
                     /locus_tag="VW077_001564"
     CDS             3065..3295
                     /locus_tag="VW077_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455559.1"
                     /translation="MVRNKIGDLTNTLFAQLETLDDRDLTADELKVELQRSKQMVAIS
                     GQILQAGQLALDAEKFKDKVGEVNAPIALLEG"
     gene            3309..4031
                     /locus_tag="VW077_001565"
     CDS             3309..4031
                     /locus_tag="VW077_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU9455560.1"
                     /translation="MHEICPGRLTPEVTRLLNEKFGTNYNKSQIGGVRKRLGLAVGKV
                     YQGRLLTKEQHDYLVLIQKNKISRNVANEMNQKFGVSLTEKQIKSYRRNNNLHSGLTG
                     RFEKDQTPHNKGKKYPNMPKNSGQFKKGNRPPNYVPVGTINYTTDGYPKEKIGEPNQW
                     VLKHRKVWEDHHGLIPKGYSIVFLDGDKTNYDISNLACLSKNEIARMNQNHLFTSNAD
                     LTKSGIGLTKLTNKIREVEKNG"
     gene            4024..4506
                     /locus_tag="VW077_001566"
     CDS             4024..4506
                     /locus_tag="VW077_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEU9455561.1"
                     /translation="MASLYELTGQFLTIYQLDIDDETKADTLEAIDWQEQFEQKAEGY
                     AHVIKNLEADVAMYKAEEESFKAKKQAAQKKLDYVKDNIMAAMNVTGQTEVKSGALII
                     KIAKNPESVKVNENDLPKKYFTKKVTLAPDKKTLKELLKSGKKVKGAELIRTEKLVIK
                     "
     gene            4506..4865
                     /locus_tag="VW077_001567"
     CDS             4506..4865
                     /locus_tag="VW077_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455562.1"
                     /translation="MELMNKTRVTDSLAVVIGLESIEVLVTEGFLFDVAIRFVKVDET
                     NLDQGNEKPVFTPEYKLVTVAKYKEKPIFESEEDIRKFEKQAKEVKSLFAFAKVNKQN
                     WFNTALYPGVLTEKVGV"
     gene            4865..5350
                     /locus_tag="VW077_001568"
     CDS             4865..5350
                     /locus_tag="VW077_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455563.1"
                     /translation="MKILAIDPSSNKIETSTTGVVLLDNARLVDSWVVSYGMRGFADW
                     FHEIGTNLEFDVVIVEEFKARDNEKSKDNSVAETIAYIQLCYPEAILQFNAGYKSDIP
                     NDLLKILDLWKFEKSHHQDIRAAARLGLFWAMRNDIEEVVHDIGKVVSEYHNNAKKVA
                     S"
     gene            5313..6506
                     /locus_tag="VW077_001569"
     CDS             5313..6506
                     /locus_tag="VW077_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HEU9455564.1"
                     /translation="MSITITLRKWQAEAIKRSEHLSNGIFLEALGGRGKTICALAIAK
                     HKKAKKIIITNNRLAILNGWIDAVKFMNFDKGVEIIIQTDRYLQNQVKKGHKLDCDVL
                     IVDEWQNMSSDKQVALYRKIKRKYTIGLSATPIRKKGQNFYPLEKTVFGWATPNNKFD
                     WQKTHGKMVYDPFSYSKEKWEDFQNYESYISSLPNFFRWEEIEGIENAVENNGFEIKF
                     YQKRVASGNPEKLAEFRKLNLVTVDGKTAMAKQSFGRKTFERYLNQTGVAVDFPKLKP
                     VNADTPLMLQLDGLIERAPHDMLIVSKSKQIVNVISERHPEIGIWTGDIQEGLYKKFV
                     VATSQVLGVGVDDLQHKYQTIVVLDPVEEGSGEYDDYRQLLWRITGSRQQHDVNVIEF
                     YYKES"
     gene            6525..6842
                     /locus_tag="VW077_001570"
     CDS             6525..6842
                     /locus_tag="VW077_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455565.1"
                     /translation="MNKTTEMIVFRSRKTGEFLNSYKDRSSLAFAADFCSLEYCLKLP
                     RKKYEDNKKTYKALAAAFDCEIVAVEAEYKLTYPNGSEVEPIKRDRSSIEDMIKDIIG
                     GVL"
     gene            6842..7618
                     /locus_tag="VW077_001571"
     CDS             6842..7618
                     /locus_tag="VW077_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9455566.1"
                     /translation="MAFTLPANKPQVPKDTPRNFFIYGETMSGKSYLANEFPNPIVLN
                     TDGNAEANTVPSIQLINEKDDKGRITNSVIKQLGDILLALQTQKHSYETVVIDVIDDV
                     IEMIKIAVCDELTPVGKPRLKSLSEIPYGKGYDFFNQAITELVIDLKALPMNVIYISR
                     QVSEYDDNGNATKDKPSLKDKYVNLINGNSDLMIHTEKLGNNYNREVDRKRKTYYVNH
                     VDDKAILKILATIRGAVEPAKTEKTKEAPKKEVASDDELF"
     gene            7638..8141
                     /locus_tag="VW077_001572"
     CDS             7638..8141
                     /locus_tag="VW077_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455567.1"
                     /translation="MSLLDIAKSIKKEGFDPRKDSANGPAPIPAGTYPVVLKKATFNV
                     SDKGWESLGYQFEIRGGDYSGRSEFATFGTLTEWNGKDLNWAVERTMKFFIKALVLAG
                     DSMQGNEEDGKALEEALKRKAVGSYYNLVISVTKGKDGREFRNYDLEEEAQPLTEADI
                     DDDDLPF"
     gene            8314..9135
                     /locus_tag="VW077_001573"
     CDS             8314..9135
                     /locus_tag="VW077_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEU9455568.1"
                     /translation="MSSMKEYALQYQKLGFSVIPINPKNKMPLIDFADKPAMTPSEIE
                     NFWDGYPNANIALKITNFFVIDIDKHGKSNGFESLKKWKHLNLIEPTLQAKTASGGKH
                     LFYFKREDEPITQMIGFLPGVDIKAHENNYVLVAPSATDKGQYEWDLEKSKEGGTMVT
                     PSKDLIQSIKKQYGETHGYKYDGKDGLRDLVRRSHTRDRTQTTDLFETIALGFGDEGG
                     RNDKLAKFVGGLLYRAVDDGVVVQLARLANANSPNPLPEKEMMRTIESMIKKDRR"
     gene            9138..10481
                     /locus_tag="VW077_001574"
     CDS             9138..10481
                     /locus_tag="VW077_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9455569.1"
                     /translation="MIGNVVSIDSQPKMITTAKGDIKANSPSNVLMSFKADDQLSIYL
                     KHNDFSQEHELLKDIKIGNTLFKKGELPSNFDSVVKVYFESVLGVAFSNQAMLDGMET
                     FFSERSYNPVIEYMERATEKWDGRNRIDRMLQVYLGAEDIPLVSKIAQMWLVGAVAKV
                     YDPYVKFDYVLDLVGGQGVGKTSLLQKLGGEWYTDAVTDFSNKDNYDIMLKSLIVNDD
                     EMVASNRMSFAETKAFISKTSLRYRKPYMKRTEEFAKNFILARTTNQKEYLKDKTGER
                     RFLPIMADSKQQKKHPMEIDPDTIEQIWGEAVTIYRAGADLMFDENTEDELNIYREQF
                     MYRDEVELQVLEYLDMPVPENWQNWSIQQQHQYTSKYFDNSSDFDPGSKKLDKVSTRE
                     MMYNLFMRNSNDRKLSTKINMIMDNHPDWKKSVFRAGGKNTKGFVRVKNSEKTNR"
BASE COUNT         3751 a         1812 c         2268 g         2799 t
ORIGIN      
        1 agtgtttttg ttatgatttt attttagact ttattataga ctttgtcaat aaaaaagtac
       61 aaaaaatagt tacattttta gttgtttctt gtttacttgt tccttgacaa ttgaatagag
      121 catgtgagat aataaagggg aattaaggat tagttctata tcatggacta gaaaagaccc
      181 caggctaact tccacattaa gcttggggtc tttttttgac actatttgtc cttgttcagc
      241 catttatcag ctaaacgaag aacgacaccg accacaatcg gtccgatgat agttttaagg
      301 attagttcca tcatgggcta tctcacctcc tttcgtaggc ggtgtagaag tgccgtaaaa
      361 tattatacca catttctaat tcagcttgtc aaaggtttct tttgacaact taaaatagat
      421 gtgagataat ataggggaag tgaagatgta gtctactaca actacaaaaa agcccctgct
      481 gataaccaca aaagcaaggg ctttttctag tctacgctag aaaggtgggt tagtcgctat
      541 ttcttgttta gccatttttc gatgactatc aggacgatac cgaccaccaa gggtagaata
      601 atatttgtga agatgtaatc taccataggc tccacctccc ttctaaggca gatgtgccgt
      661 cactattata ccacatgctc tatcagttag atagggcatt ttttattttt gaaaaaagga
      721 ggaacgtgtc gatggtaagt attcttaaaa atttagaaca agaaaaagac caccttgaaa
      781 aaatcattaa ggtagtcagc gctggtggta aatttctgag attgccatat caaaaaaagt
      841 cacgctcgat tagtgagaat ctgaaattga tttctcaaaa tcttgataaa ttgagcgagc
      901 aagttcaaca aaccacgaat cagcattcat gatttcaaga tgacgaaaga aacctgtttt
      961 ggtttccagt tcagaatcgt gtgcataacg taatatttct ctagcaaaga tggtttcaaa
     1021 atcaaaatct ttaccatcat cgtagatgtc gcgtttgctt gctttgagta aatattcttt
     1081 aaaagtcata aggttaactc ctttctgctt atattatagc agaaaaagaa gaaagaaaaa
     1141 gaaaggagaa aatatgccag atattgcaaa cggtcgcgaa agagttattg ctttcttgaa
     1201 agagaaaggc attaaaaaag caactctagc ggttgcttac ggctttaaac gacaggaagt
     1261 gacaaacatt ctaagtggaa cgactaaagg tccacgagcg aacagtttca ttcttcaggt
     1321 tattgaagat tatgggattg agtaggaaag atttgaggag taggaaaatg agaccaagac
     1381 gatatccgta tagtgggaaa aaagagtcca cctttgtaaa ggccgaccct gagttagttg
     1441 aaaaactttt aagaaacact agttttcttg agtgtttaca aaaaaagcct atcaattttc
     1501 agatagactt agaagaattt aagcgtctta gctatgaagc cattcatgat acttctcaag
     1561 taactcaaca gtagttatta cagaagtcaa accactgacc ttccccagtt gcaatccgtc
     1621 tgtatggtca atctgtttag tagcttcatt agctttagca gagatagctt gcatatcttc
     1681 agctgttaaa gattctcgaa aatctttaaa ggatttcata aaaactcctc ctttctattg
     1741 gaattttgac taaaacggtg agaggtccta gtcgagatta ttatagcaat ttaggaggat
     1801 attacatcgg tcttgaggct gatttttgga ggcaatattg gaagataaaa tcatagaact
     1861 tgctgattac ttcatcagcg aatctacaac gtacagagaa gcaaaaatag cgtgtgagaa
     1921 gctattgaaa caagtcagcc atgagataga actcagggcg atggaaagta ggacagtcta
     1981 gaagacaaca aaaagcacct gacggaaatc aggcgcatac ttaaatattc aacatgatta
     2041 taacacgaaa ggagcaaaaa tggaagtagt tgaaattgta agaattaaag atgtgattat
     2101 tgaaaaagtc tctgctaatg atgaagagtt aaaacgtatc tttggatgtt caaaacgaca
     2161 agcaggagag cgaagaagag aaatgcaaaa actccctagt cagcaaaaac atcttttgga
     2221 tagtggacaa cttgtaacga ttaaaggttt ctatgaatac ttgcaatatc gtggaactaa
     2281 agcttggaaa aaagaaatgg aaacaagcaa gaaaatgagg tcagcaggat gaacctacta
     2341 tcaagaatca aaaactattt ttcggaagag gtcaaagaaa ctaatctcga ctggaaagag
     2401 gtcgctttag acctcaatca atcactaatt gaaacacagg aaaaacttca agaagcgaat
     2461 caagaaatcg cagacttgaa gaaaatcgta gcaatctaca aagaaaagga gaaagaaaaa
     2521 tgatggaata catttacctg gtaataatcg taggaattgg actatggtcg ctagtaaata
     2581 aactagatga ccacgctgaa atgaaacaaa aagagcgcca gttgatggca aacaatgtcg
     2641 cacggatgaa tctgagaaat tcagataagc aatttactta tgatgtagaa ccgcctgaag
     2701 ggttgaaata aggaggagaa acatgactca agcggaacaa attagggaat attatagaga
     2761 gcaccctgct gcctcatgtg atgaagtggc tgaggttgtc ggtacaacaa aaagcaacgt
     2821 aagtgcaaac ctggccaaag acatcaaggc aggcagatgc gttcgcttgg aagacaagtc
     2881 atacgactac tccccttact ttaatcacac acaagcgctc actgagttgg ttgattggaa
     2941 gaatgatacc agacgtgagt gggtggatat gctgacaaga gcagcagaga aagaaacgga
     3001 tagcaacgtt atgcgtttgt taatcaaaga agcaaataaa ttgatgaaag aggtgactaa
     3061 gtagatggta cgaaataaaa taggtgattt aactaacacg ctcttcgctc aattagagac
     3121 tctggatgat agggatctta cagcagatga attaaaagta gaattacagc gctcgaaaca
     3181 aatggtcgca atctcaggtc aaatcttaca agcaggtcaa ttggcgctag atgctgaaaa
     3241 attcaaagac aaggtaggtg aagtcaatgc cccgatcgct ttgctggaag gatgagtaca
     3301 cggagtacat gcatgaaata tgccctggtc gtttaactcc tgaagtaacc aggttgctga
     3361 atgaaaaatt tggtacgaat tataacaaga gtcaaatcgg tggcgtacgc aaacgtctag
     3421 ggttagcagt tggaaaagtc tatcaaggtc gattgctgac aaaggagcaa catgattatc
     3481 ttgtattgat ccaaaaaaat aagatttctc gtaatgtcgc aaatgaaatg aaccaaaaat
     3541 ttggcgtatc gctaactgag aaacagatta agagttatcg gagaaataat aatctacata
     3601 gtgggttgac gggaagattc gagaaagatc agactcctca caataagggg aagaagtacc
     3661 ccaatatgcc aaaaaacagc gggcagttca aaaaaggtaa tcgacctccg aattatgtac
     3721 ctgtcggcac tatcaactac acaacagacg gttatccaaa agaaaagatt ggagaaccta
     3781 atcaatgggt tttgaaacat cgtaaagtct gggaggacca tcacgggctg ataccaaaag
     3841 ggtactcaat cgtttttctg gacggtgata aaacaaacta tgatatttca aatctggcat
     3901 gtttatctaa gaacgaaatt gctagaatga atcaaaatca tttattcacg tccaacgctg
     3961 atttgactaa atcaggtatt ggactaacaa aacttacaaa taaaatcaga gaggtagaaa
     4021 aaaatggcta gtttatacga actaacaggt cagttcctga caatttacca attggatatc
     4081 gatgacgaaa caaaagcaga cacgcttgag gccatcgatt ggcaagaaca atttgaacag
     4141 aaagcagaag gatatgccca tgttatcaag aatctagaag ccgacgtggc catgtacaaa
     4201 gctgaggaag agagcttcaa agccaagaaa caagcggcac agaaaaagct ggattatgtc
     4261 aaggataaca ttatggcagc tatgaatgtc acaggtcaaa ccgaagtcaa gagtggtgcc
     4321 ctgattataa aaattgctaa aaatccagaa tcagtcaagg tcaacgaaaa tgaccttccg
     4381 aaaaaatatt ttacaaaaaa agtgacgctt gcgccggaca aaaaaacact caaagagttg
     4441 cttaaatctg gcaagaaagt caaaggagcc gagctcatta gaacggaaaa gttggtgatt
     4501 aagtaatgga attgatgaat aaaacacgag taacagattc actagcagtt gtgattggac
     4561 tagaatcgat tgaagtactt gttactgaag gttttctatt tgatgttgcg attcgttttg
     4621 taaaagtaga cgaaacaaat cttgatcaag gaaatgaaaa gccagtattc actccggaat
     4681 acaagctggt cacagttgct aaatacaagg aaaaacctat ctttgaatcg gaggaagata
     4741 ttcgaaaatt tgagaagcaa gcaaaagaag ttaaatcgct atttgccttt gcaaaggtaa
     4801 ataaacaaaa ttggtttaac actgcccttt atccaggagt gctgactgag aaagttggtg
     4861 tttgatgaaa attttagcta ttgatccaag cagtaataaa attgaaacca gcacaacagg
     4921 agttgtcttg ttggataatg caagattagt tgatagctgg gttgtctctt atggtatgag
     4981 aggtttcgct gattggtttc acgaaatcgg aacaaatctt gaattcgatg tagttattgt
     5041 tgaagaattt aaggcgaggg ataacgaaaa gtcaaaagat aatagcgtgg cagaaaccat
     5101 cgcttatatc cagctttgtt atccagaggc tattcttcaa tttaatgcag gttacaagtc
     5161 ggatattcca aacgatcttt tgaaaatctt agacctttgg aaatttgaaa aaagtcatca
     5221 tcaagatatt agagcagcag caagacttgg attattttgg gcaatgagaa atgatattga
     5281 agaagtggtt catgatatcg gaaaggtggt gagtgagtat cacaataacg ctaagaaagt
     5341 ggcaagctga agcgattaaa agaagtgaac atttatctaa tggaatcttt ttagaggctc
     5401 ttgggggcag aggcaaaact atctgtgcac ttgctattgc aaaacataaa aaagctaaaa
     5461 aaatcatcat cacaaacaat cgactagcta ttctgaatgg ttggatagat gcagtcaagt
     5521 ttatgaattt tgataaaggt gttgagatta tcattcagac agatagatat cttcaaaatc
     5581 aagtcaaaaa ggggcataaa ttagattgtg atgtgctgat agtagacgaa tggcagaata
     5641 tgtcttctga caaacaagtg gccttatatc gcaaaataaa gcgaaaatac acgataggtc
     5701 tttcagcgac accaattcgg aaaaaaggac aaaatttcta tccgcttgaa aaaacggtat
     5761 ttggttgggc aaccccaaat aataaatttg actggcaaaa gactcatgga aaaatggtct
     5821 atgatccatt tagctattca aaagagaagt gggaagattt tcaaaattat gaaagttata
     5881 tctcgagctt gcctaatttc ttccgctggg aagagattga aggaattgag aatgcagttg
     5941 agaataacgg ttttgagatt aagttttacc aaaagagagt cgcctctggc aatccagaaa
     6001 aacttgcaga atttagaaaa ctaaatcttg taacagtgga cggcaaaact gcaatggcca
     6061 agcaatcgtt tggaagaaag acctttgaac gctaccttaa tcaaacaggc gtagcagtcg
     6121 attttccaaa attaaagcca gtaaatgcgg atacgccatt gatgttacaa cttgacggtt
     6181 taatcgaacg agcaccacac gatatgttga ttgtcagtaa atctaagcag attgtcaacg
     6241 tcattagcga gcgccatcct gaaattggaa tctggacggg cgatattcaa gaaggacttt
     6301 ataagaaatt cgtggttgct actagtcaag tgttaggtgt cggagtagac gacttgcaac
     6361 acaaatacca aactattgtc gtattggatc cagtagaaga aggttctgga gaatatgatg
     6421 attatcgaca attgctctgg cgcataacag gaagtcgtca gcagcatgat gtaaatgtaa
     6481 ttgaatttta ttataaagaa agttaaaaaa aagaggaaaa caaaatgaat aaaacaactg
     6541 aaatgatcgt atttcgtagc cgtaaaactg gagaatttct taattcttac aaggacagaa
     6601 gttctttagc atttgcagct gacttttgca gcttggaata ttgtttgaag cttcctcgta
     6661 aaaaatacga agacaacaaa aagacttaca aggctcttgc tgcagctttt gactgtgaaa
     6721 ttgtcgcagt tgaagcggaa tacaaattga cctatccgaa tggatcagaa gttgaaccta
     6781 tcaagcgtga ccgttcatca attgaggaca tgattaagga tattattgga ggggttctct
     6841 aatggcattt acacttccag caaataaacc acaagttcct aaagataccc cacgaaattt
     6901 tttcatctac ggtgaaacca tgagcggaaa gtcttatctt gcaaatgaat tcccaaatcc
     6961 aatcgttttg aacacagacg ggaatgcaga agctaacact gttccaagca ttcagctgat
     7021 caatgaaaaa gatgacaagg gacgaattac caattcagta attaagcagc ttggagatat
     7081 cttgcttgct ctccagacac agaagcactc ttatgaaaca gtcgttattg atgtaattga
     7141 cgatgttatt gaaatgatta agattgcagt ttgtgatgaa ttaaccccag ttggtaaacc
     7201 tcgcttgaaa tccttgtcgg aaattccata cggcaaagga tacgacttct ttaaccaagc
     7261 tatcacagaa ttagtcattg acctcaaagc attgccaatg aatgttattt acatcagccg
     7321 tcaggtatct gaatatgatg acaatggcaa tgccaccaaa gacaaaccaa gcttgaaaga
     7381 taagtatgtc aatcttatca atggaaactc tgatttgatg atccacactg aaaaactcgg
     7441 caacaactac aaccgtgagg ttgaccgcaa gcgtaagacc tactatgtga accatgttga
     7501 tgacaaggct attttgaaaa tcttagcaac tatccgtggg gctgttgaac ctgcaaaaac
     7561 cgaaaagaca aaagaggcgc ctaagaaaga agttgcctct gatgatgaac tattttaaga
     7621 aataaaggag aatacacatg agcttactag atatcgcaaa atcaatcaaa aaagagggct
     7681 ttgacccacg caaagacagc gccaacggtc ctgcaccaat cccagctggt acttatccag
     7741 tagtcctgaa gaaagcaact ttcaacgtat cggacaaagg ctgggaaagc cttggttatc
     7801 aatttgaaat ccgtggcggt gattacagtg gacgctctga atttgcaaca tttggcacac
     7861 tgactgaatg gaacggtaag gaccttaact gggcagttga acgcaccatg aaattcttta
     7921 tcaaagcctt ggtccttgct ggcgacagta tgcaaggaaa tgaagaagac ggtaaagcct
     7981 tggaagaggc tctaaaacgt aaggcagttg gctcttacta caaccttgtt atctctgtga
     8041 ctaaggggaa agatggccgt gagttccgaa actatgacct tgaagaagaa gcacaaccgc
     8101 tgactgaagc tgatattgat gacgatgacc tcccttttta agaaataaca agttctgggt
     8161 cattgatgaa actgatgaaa gattaggacc attcaataca tttgaagagg cttatcaatc
     8221 gttgttattt tatttaaaaa tgactgaaga tgaatatcaa tcaaattata tggcccaaga
     8281 acttgtttat atttacaaaa aggagaagta atcatgtcgt cgatgaaaga atacgcatta
     8341 cagtaccaaa agttagggtt ctcagtcatt ccaatcaatc ctaaaaacaa gatgcctttg
     8401 attgattttg ctgataagcc agccatgact ccatctgaga ttgaaaactt ttgggacggc
     8461 taccctaatg caaacattgc cctaaagatt accaacttct ttgtcattga tattgacaaa
     8521 cacggcaaat cgaacggttt tgaatcgcta aaaaaatgga aacatctaaa tttaatcgaa
     8581 ccgacactgc aagctaagac ggctagtggc ggtaaacatc tattctactt caaacgagaa
     8641 gatgagccga tcactcagat gattggattc ttgcctggtg ttgatattaa ggctcacgaa
     8701 aataattatg tgttagtcgc accctctgcc acagataaag ggcagtatga gtgggatctg
     8761 gaaaagtcta aggaaggtgg cacgatggtc actccttcaa aagatttaat ccagtctata
     8821 aaaaaacagt atggcgaaac tcacggttat aagtatgatg gtaaggacgg tcttagggat
     8881 ttagttagac gttcacatac tagagaccga acacagacta cagatctctt tgaaaccatc
     8941 gcccttggtt ttggtgatga aggtggacga aatgacaaac tagcaaaatt cgtaggtggt
     9001 ctcttatatc gtgcggtcga cgatggtgta gttgttcaac ttgcaagatt agcaaatgca
     9061 aatagtccaa accctttgcc tgaaaaggaa atgatgcgta ctattgaaag tatgattaaa
     9121 aaagatagga ggtgattgtg attggtaatg tagtaagtat tgactcacaa cctaagatga
     9181 taacgactgc caagggagac atcaaggcca acagtccaag taatgtgttg atgtctttca
     9241 aagctgatga tcagttgagt atttacctaa agcacaacga tttttcccaa gagcatgaac
     9301 tccttaaaga tatcaagatc ggcaacactc tttttaaaaa aggtgagctc ccttctaact
     9361 ttgattcagt cgtaaaagtt tactttgaaa gtgtgttagg tgttgctttc tcaaaccaag
     9421 cgatgcttga tggcatggag actttctttt cagaaagatc atacaatcca gttattgagt
     9481 atatggagag agcaactgaa aagtgggacg gcagaaaccg gattgaccgc atgcttcaag
     9541 tatatctcgg cgctgaagat atccctttag tttctaaaat cgctcaaatg tggctagttg
     9601 gtgcagttgc taaagtttat gatccatacg ttaagtttga ctatgttctg gacctggtcg
     9661 gtggacaagg agttgggaaa acgtccctcc ttcaaaaatt gggtggcgaa tggtatacgg
     9721 atgccgtaac agatttctct aataaagata attacgacat tatgttaaag agtctaatcg
     9781 tcaacgatga tgaaatggtg gccagtaatc ggatgagctt tgcagaaact aaggccttta
     9841 tttctaaaac tagcctacgt tatcgtaaac catacatgaa acgaacagaa gaatttgcca
     9901 agaacttcat cttagccagg actactaatc aaaaagaata cctcaaggac aaaaccggtg
     9961 aacgtcgatt tctcccgatt atggcagata gcaagcaaca aaagaaacat ccaatggaaa
    10021 tcgatcctga tacaatcgaa caaatttggg gcgaagccgt tacaatctat cgtgctggtg
    10081 ctgatttgat gtttgatgaa aatacagagg atgaactgaa tatctaccgt gaacagttca
    10141 tgtatcgtga tgaagttgaa ttacaagtgc ttgaatatct tgatatgccc gtccctgaaa
    10201 attggcaaaa ctggtctatt cagcaacaac atcaatacac aagtaaatat ttcgataata
    10261 gtagcgactt tgatcctgga agcaaaaaac tagataaggt ctcaactcgt gaaatgatgt
    10321 acaacttatt tatgagaaat tcgaatgaca ggaagctgtc aacgaagatt aacatgatca
    10381 tggataatca tcctgattgg aaaaaaagtg ttttccgggc aggaggtaaa aatacaaaag
    10441 ggttcgtaag agtgaagaat tcggaaaaaa ctaatcggta gcaattaaaa aattattggt
    10501 agtcatcggt agcagttgag ggggagatcg gtagcattct accgataaaa taggacatcg
    10561 gtagcacatc ggtagcagtc taaccccttg atattactga cttttattta atatttatat
    10621 ataatgctac
//
LOCUS       DAVKGU010000068        10555 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKGU010000068 DAVKGU010000000
VERSION     DAVKGU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.068"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(71..1495)
                     /locus_tag="VW077_001575"
     CDS             complement(71..1495)
                     /locus_tag="VW077_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9455570.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2040..4319)
                     /gene="pulA"
                     /locus_tag="VW077_001576"
     CDS             complement(2040..4319)
                     /gene="pulA"
                     /locus_tag="VW077_001576"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9455571.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4431..6389)
                     /gene="ligA"
                     /locus_tag="VW077_001577"
     CDS             complement(4431..6389)
                     /gene="ligA"
                     /locus_tag="VW077_001577"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9455572.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDSFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRSLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6482..7660)
                     /locus_tag="VW077_001578"
     CDS             complement(6482..7660)
                     /locus_tag="VW077_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9455573.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVCLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFFPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSLSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7740..8603)
                     /locus_tag="VW077_001579"
     CDS             complement(7740..8603)
                     /locus_tag="VW077_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9455574.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8606..10507)
                     /locus_tag="VW077_001580"
     CDS             complement(8606..10507)
                     /locus_tag="VW077_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9455575.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVEEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGDELDKLMSDYDHLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3155 a         2335 c         1903 g         3162 t
ORIGIN      
        1 tttttctata attattatta ttttatagca aaaaaataaa accaggaaaa caatttcctg
       61 gttttacacg ttatttcaca tcaaaaacaa tggatttgac atttgtcatc gcttcaatgc
      121 tatatttaat tccttgcact ccagctccag aacctttgac accaaggaat gggaaattat
      181 ctggaccacg ctgggtttta ttattaatgt ggactgtacc tacttcaagt ttttcagcaa
      241 tttcaaaggc ttttttgaaa tcatttgtaa agactgatga ttgaaggccg aattcagatt
      301 cgttggcaaa ggcaatagct tcctctacac tagccacacg aatgattggt aaaacaggac
      361 caaatggctc ttcccatgcc actttcatat cttttgtaac ttggtcaaaa agcactggcc
      421 agaggagatt gccctcacgt ttgattggtg taagagcctg agcctctttt tcttgtgcat
      481 cctcaatcaa gccccaaatg aagtcggctg aagcattatc aataacaggt gtaatatcag
      541 cattgtcaaa tggatcacca actgttaatt tagaaacttc ttcctgaagc aaagtagcta
      601 atttatctgc tacactttcg agaacaatga cacgtttaat ggccgtgcaa cgttgtcctg
      661 agtagctaaa ggctcccgca acaatttgct tggcagcatg ttccaaatct gcatcttcta
      721 gtacaagagc tgcatctttc ccaccaagtt ccaacatgat aggacgcata ccagctaaac
      781 gaccaatacg ttctccaata ggagttgaac ctgtaaagtt gatgaagttg acttctttgt
      841 gctcaatgat ataatcccca atttctgaac cacgacctgt aatggtgttg aaaacacctg
      901 ccggaatccc tgcttcttca aatgctttag ccaacaagag tccagaaatg gaaccttgtg
      961 ttggtggctt aaacatgacc acattccctg caatcaaggc aggtgcaatt ttagaagcag
     1021 ataaattaac tggataatta aagggagcaa tcgctagcac gataccaact ggttcacgac
     1081 ggacaacagc cagtttgttt ttacttgctg cctcaaaacc accaccttcc attgcttgtc
     1141 cagtgatacg gagaccttcc tcagcagcat aacgaatcaa gtctgctgta cgcactactt
     1201 ctccaattgc tgctttaatc ccttttgcta cttctttggc aaggatagta ccaatttctt
     1261 ccttatcgcg ttctaaaata gctgctgttt tatgtaaata agccgcacgt tcaactgctg
     1321 ataaagctcg ccatgctggc agggctgcac gcgcagcttg catagcctca tcagcttcag
     1381 tctgagtcat ggctggaact gtacccaatt cttcttgatt gattggtgaa taaatcgtaa
     1441 tttcttgttc agatgatttc cattttccat ttactaaatt ctgatatctt gtcaaattct
     1501 tgtcctcctg aaaatgatta agatatattt taccaaattt tcagaaaatc acaacccttt
     1561 ttgcaaaatt tttgagatta ttttcacaaa cttgattttt caaagtatac tcaataaaaa
     1621 taaaaaaaat ccactacgtc aaggcgaggc taatgtggtt tgaagaaatt ttcgaagagc
     1681 gtgaatgagt atcatctata gtaaaataaa aaaactgaac aatttggttg gggacagcca
     1741 aaccaatttc tcacaatgtt tcagaaacaa gggtgtgcta ctccaatttc agcctactat
     1801 aactgtcata gattgctgaa acaaagtcta ggtaaaagtc ttcataataa aaagacctcc
     1861 tatcaagtgt tcaaaaactt tgataggagg tcttgttttg tgaaaatatt tatcaaattt
     1921 tctatacaag tgagctgtta gccaggttct ttctattctt tcaatttcaa tgaatggatt
     1981 ttttactaat actcataact gggaatttgt ctgtgtaaaa atagcgagat agatggtatt
     2041 tataaaacac tcaagacagc tagactaata tcatttaaaa cattatcttc ttttgagcga
     2101 ctgttggtta ccaacatagc taaatttcct gcattttcaa attgataggg ttctgattta
     2161 gcattcacaa ccaccaagag gtgttctttg ccgtgaactt catagataag gtagccgcta
     2221 tgttcaatcg cagaatgcac aaagacatga tggtaaattt catcatagct agagtaagaa
     2281 aaggcaccag tttttgtctt caatcggatg acttgacgga taaactcaat actgtcttga
     2341 cgctcattaa tcaagttcca gttcacttgg ttcacactgt caggagcatt atagctattc
     2401 atcgcacgct ctctatcatc atgggtcaac tcaccatttt caccagtcgc aaccagtttg
     2461 gtacgaccaa attcttgacc gatttccata aaggccatcc cctgcatgag cagattcatg
     2521 gctgtggcag tttcgacctt gcgcatgatt tgctctgaac tttggtctgg atgaagggtt
     2581 gccaataaat cgtgaagatt gtaattgtca tgggcttcta catagttaag cacctgattt
     2641 ggatgtgtat agcttcctaa ttcacgactt cctaggattg ctttagctag aattggctct
     2701 gtcgcagcac cactgacaaa acctgacttg atagcaccat aaacttctcc ccctttgaca
     2761 gcatcgcgct gattgtcatt aaagaaacca atatttggca tctggtaggc attgtccttc
     2821 ttggccttat cataaggggc aagacctgtt cccatatccc atccttctcc atagaggata
     2881 atgttggagt cgatttcatc caagctttga cgaatcatct gcatggtctt gacatcatga
     2941 atccccatca agtcaaaacg gaagccgtca atattatatt cctgcaccca gtatagaaga
     3001 gaatcaatca tatacttgcg aaacatttcg tgttcactgg ctgtttcatt tccaacaccc
     3061 gttccattct ggaaggtacc atctggattc atacgataat agtaatcagg gactgttgtt
     3121 tggaatggtg catcaacaac tgagaaggta tggttataga ctacatccat aatgactcca
     3181 atacccgcat cgtgataagc ttgaaccatc accttcaaat cacgaatgac ctgagctgga
     3241 tcatctggat tagttgaaaa actagtttct ggcgcgttat agttttgtgg atcataaccc
     3301 cagttgtagg ttacatttcc atcctcatcg tattctttat gacggtctgc aattggttgc
     3361 aattgaacat aattgtagcc cagcttcttg atgtaatcaa aagcagttga ctggccgtat
     3421 tggttaactg ttccagcctg agcagcaccc aagaaagttc ctcgaagatg ttcatctaca
     3481 cccgatgtag gtgatttagt caaatcacga atatgcattt cacagataac tgccttacat
     3541 ggattttcca agcgccaagt agcctccgaa ccgtgcttaa cctcgaagtt ttcaacttgc
     3601 ttttctacat ggctcagaat agctgaacgt ttgccatcag ggctggtcgc gattgtataa
     3661 ggatcacgtg tcagtgtttg gtgatgaggg aattggactt gatactgata agccttacct
     3721 accaaatctt cctcaacatc caaactccag acaccgattg tattgtcctt atgattataa
     3781 gagtagctat tgcctctttt catctcaaaa gtcttccaaa cgggtgcatc attagcagct
     3841 gattcataaa cgacaacttg cacttctgtc gctgtaggtg accagaggga aaaatgagcc
     3901 tgattgtcct ctacacggca acccaattct ccttggtaac cccaatgatg gtcaaaacta
     3961 gcactgttaa tggccttatc aaaggcaaaa ggattttgat ttttatagaa aggactggca
     4021 atagcaggat tttcagagta ataaatccta tcatcgcctt ccaaaatcca gacctctgtt
     4081 aataggggat agtgattaaa acggatagaa tattctttac tagtttgacc tgtatgaacc
     4141 acaaaattca agctttctat aacatgtgaa cttgggtgtt caaagctaaa taaagctcca
     4201 aaataatctt ctttgtaggt tagcaaatca attcgttgat cctgactttt tacaaaggag
     4261 caagtgtcat attctccatt cttacgatgg taatgaatgc gcatagggta gttatacatt
     4321 ttttattttt cctttttact ttgtttctat ttcactaata aatttttgtc aatctcgtct
     4381 caattaacag acatagtcat attctctaaa ctctgttttt aaacgatcca ttacaaactt
     4441 tctagccatg cctcatctct gacctggata ccaagttctt gtgctttttg cagtttactt
     4501 ccagcgtctg cacctaccac gacgaggtcg gtctttttag aaacactacc tgtcactttg
     4561 gcacccagac tttcgagttt acttttagct tctgagcgct tgagtcgttc caattttcct
     4621 gtcaatacca cggtcaaacc tgacaaggcc gcatccgcta ctaccgtctg tcctttatag
     4681 tccagattga ccccagtttc tttcaattct ctgagcagaa tttcagagcc ttctgtcgca
     4741 aaataagtct gaagactttt ggcaatcacg ccacctagac tttcaatact agccacttcc
     4801 tctgaatctg cctgagacag attttcaatt gaatggaaat attgaagtaa aagctgacta
     4861 gccttgcttc cgacatgacg aattcccaaa ccaaataaga gcttctcggc agaattttcc
     4921 tttgatgctt ggatagcctg atacagttta gcagcggact tttccttaac cccctctaaa
     4981 aggaggaaat cctcttcttg caaacgataa atatccgcca catccttgac taaattagca
     5041 gcaaaaagct tctcaacaat agatggacca aggcctgtaa tattcatagc atcacgagaa
     5101 gcaaagtgaa tcaagccttc catgatttga gcagggcaac gcggattgat acaacgtagg
     5161 gccacttcat cttcaaagtg caacaagtca gagttacaac ttggacagtt tgtagggata
     5221 tctagttttt cttcagaaac ccgtttggac tctaccacac gtaaaacggc agggatgatg
     5281 tcaccagcct tatatacaat gaccgtatcg tcttttcgga tatctttttc agcaatataa
     5341 tctacattgt gcagggtcgc acggctaaca gtcgtaccgg caagttgtac tggtgttaga
     5401 ttagcagttg gagttacaac accggtacgg ccaactgtcc agtcaactga taagagttga
     5461 gcttcttttt cttcggcagg gaacttgtag gctactgccc actttggagc cttaactgta
     5521 aaaccaagtt cttcttgact tgctaggtcg ttgaccttga ttaccactcc atcaatatcg
     5581 taaggcagat tttcccgttc ctgtcctact tcttggataa aattccagat ttcatctatg
     5641 ttttcagcca agattcgctt aggattgacc acaaaaccta gttgttctag gtacttcaaa
     5701 cccttttctt ggctatcacg agttgaaggg ctggcttctt gatagagaaa cgttgcaaga
     5761 ctacgcttgg caactactgc tgtatccaac tgacgcagag ttcctgctgc cgcattacga
     5821 ggattagcaa attcaggctc tccattttct tggcgcactt ggttaacttg gtcaaaggaa
     5881 gcgcgtggca tgtaacattc cccacgaact gtgatatcta gttcttctgg caaagtcaaa
     5941 gggatgtcct taacacgctt gaggttttct gtgatatttt caccaattga accatctcca
     6001 cgtgttaccc cagcaaccaa aatccccttt tcataagtca gcgagataga taagccatcg
     6061 attttcagct cacaaatata ggtcggatga gccacttcct tacgaacacg cgcatcaaaa
     6121 gcatctagct cctcacgtga aaaagcatcc tgcaaactat aaagaggata ctgatgactg
     6181 tatttttcaa aactatctaa aaccttgcca ccaacacgat gagtcggact gtctgctagc
     6241 acttgctctg gataagcagt ttctaactcg accaactcac ggtaaaggcg gtcatactca
     6301 ctgtctgaaa ccgagggatt atcgctggta tagtactcag tcgcatagcg attgagcaaa
     6361 gcgactaact cattcattct tttattcata agaccatttt accataaaac aagccctcct
     6421 cacaaacgag aagggcggaa aaaacactta gtttgaaatt atttttgaaa ctcaagcaac
     6481 cttatatcaa tttttcaaaa tgagttcgaa cataaataaa cgatatacaa gacaagatga
     6541 taacaccact tccaattatc aggaaagaag agagatgtac acttggcaag actgtcataa
     6601 atccttttgc aataggcata aatagaatag ctaaggtaaa aattgtactc agtactctcc
     6661 caagaaattc gctctcaacc ttggtttgta cttgagtaaa aaagtgaata ttaaaaatcg
     6721 tcataaacaa ttcacaaact aaatttccag aaagggaaag aaaagttgga agtggtaatc
     6781 ccatcataaa aactccgaca cctgtcaaag ccagtaaaat caaaagatta taaatattag
     6841 ctttaatttt actagctaga agagccccaa tgatggaacc aatagccccc atagttaaaa
     6901 tacttgcata ggctccttct gacccgtaaa gctgattcga aaagggaagt agaaattcaa
     6961 aagctgcaaa aaagaaatta acgctggaag ctaccagcaa aaggaagaaa atttcttgct
     7021 gatgccagat atagtgtaac ccatccttga tatctacaaa aatatctctc ccagtaaaag
     7081 cctttttctc ttgaactttt gcttcctctt ttggaaagaa agccactaga acaaaagcaa
     7141 tgaaaaaagt cagcgagtct agcagtagcg tcatatggag acttgcaaac tgtaaaacaa
     7201 ggaaggaaag aacaggagag ctaacaccta caacctgcaa aaccagctct aggcgagaat
     7261 tatagatcac aatctcatct ttctccacca cttcagttat gatagcttta ttggctgtgc
     7321 gagaaaaggc aaaagcaata gcctgcacaa tgttagcaac aatcaaagcg ccaatcatcc
     7381 agctatcatt ccttatgaaa gaaatagcca gacaaagaat cccacaaaca agatctgccg
     7441 tcattaaaat cttacgacga gaaaaacggt ctgaaataac tccgccaaag ggattgacga
     7501 gaatagatgt gacgagctca gaaatctgat acattcctaa aactgtctgt cctatagtcc
     7561 ccatagaagc caagcagaca ctatttccat aatcatagag catatttccc attttattga
     7621 tagccccacg gctaatcaac tgcactgcat agcgattcat attaaagctc ctctcaaatt
     7681 ttgaaactat tgtatcaaaa ccgaaaggag ctttttattt tttcccttat ttgggaaaat
     7741 taacttttga caaatttttc gtagtgttcc tgataatagg ctacttgctc tggaagacct
     7801 aacacatcaa aaatatgtat ggcctcttgc atctgcttac agccttcttt acactgtcct
     7861 ttttgatata aggcaaaacc ttttaaataa tggaaaacat tacgctcata aagcttaata
     7921 cctttgtcaa taatcttctc tgtataagcc tcaaaatagt tggcattata aaaagaagaa
     7981 tgctctaaac aatgctggta acaattgagg gccaaaatca acactaatct cttatggcga
     8041 ctaatctctt ggtaaaattc ctccctctcc ataacttctc taccaatccg agtgacatag
     8101 tctacatcgt agaaactata gaggttaccg aaaagaatca actcatacat ggtccattct
     8161 tctgttttga agagataatc tgctacctta tccaaatcat cctgcttcat atcataactc
     8221 gcatctcttt gacaaatcag accttgtagc aaaatccagt tcagctcaaa ataaagggaa
     8281 gtcgtcgaac tcttagactt ttcaagttgt tctctttgaa gcttttgaaa acctgcaata
     8341 tcgtttgaat agtaaagtgg gataatctgt gccatcatag acacatgttc atgattatga
     8401 aaattccttg ccttatccat gaaattttcg attgttacat gaatgttatc caaaatctca
     8461 aagaaacggg agactgccag gtcagactcc ccaagctcaa agcgagataa ctgagaggta
     8521 gagcaggatt cgcctgctgc ttcctttaaa gaataatttc cacttgttcg aaattcacga
     8581 aatacttttc caagatgttc catctttaca cctgatctga taattcttcc cactcaagca
     8641 tagcttcttc ctgacgatgg ctgattttgt ccagctcagc ctgtaattcc atgagtttgt
     8701 cggcatcgtt tgtttccaac atttgttcag aaatggcttg gctttgactt tctagctctt
     8761 caatttcagc ttctagactt tcgatttgtc gcatgagttt gcgaacttct ttttgacttt
     8821 ctttctgggc ctgatagtca ttgactggac ttgcttcctt tgcttgattg ctagttgaag
     8881 cttcctcagt ctgactcatt tctgctgttg ctttcttctc aacatagtag tcgtaatctc
     8941 caaggtagag agttgaacca ttctcagaca attccaaaac atgagttgcc acacgattga
     9001 taaagtaacg atcatgactg acaaaaagca aggtaccatc aaagtcaatc aaggcatttt
     9061 ctagcacttc cttactatca atatccaagt ggttggtcgg ctcatccaga atcaaaaagt
     9121 tattgttttc catagacaat ttagctaaaa gcaaacgagc tttttcgcca ccagatagca
     9181 tgccgactga ttttttaaca tcatctcctg agaaaaggaa ggctccaaga cggttgcgga
     9241 tttcaacttc tggtgtcagt ttgaaatcat tccagagttc atccagcacc gtattacttg
     9301 gtgtcagctt gctttgggtt tggtcatagt aaccaacctc aacattagcg ccaaagcgct
     9361 tttctccctt gataaacgga atttgatcca cgattgattt gataaaggtt gattttccga
     9421 taccattggg accaacgata gcaacagcat tcatcttacg aaggtctagg ttaatcggtt
     9481 gtgacaggac ttccccatca tagccaacag ccgcattttc aacagtcaaa acaacattgc
     9541 ccgacgtttt ttcagactgg aaggtcatgt tggctgcttt cttgccagct tccggcttgt
     9601 ccaaacgctc cattttttct agttgtttac ggcgagattg agcacgtttg gtcgttgaag
     9661 cacgaactag attgcgattg acaaagtctt ccagagcagc gatttccttc tgttgctttt
     9721 catagttttt tgcctcagta gctagctttt gctccttcaa ctcgacaaaa cgagagtaat
     9781 tccccacata gcgatccaag gaatgcttgg tcaaatctag cgtaattgtc gcaaccttgt
     9841 ccaagaaata acggtcgtgg ctgacgataa tgagggcacc gctatagttt accaagtaat
     9901 tctctagcca ggagatggtt tcaatatcca agtggttagt tggctcgtcc aagaccaaga
     9961 gattgggctt ttcaaggagc attttagcta gagccaaacg agtattttga ccacctgaaa
    10021 gttcagctat tttcatctgc cacatagact cgtcaaactt gaatccattc aaaatcgctc
    10081 gaatatcagc ttcataggta aagccgcctg cttggcgaaa attctcagat aagtggtcgt
    10141 aatctgacat cagtttatcc aattcatcgc cagacttttc acccatctcc aactccatct
    10201 gacgaagttg tttatctgtc cgacgtaagt catcaaagac atgaagcatt tcatcgtaga
    10261 tggtattttc agactcaaaa cggctatctt gggctaggta agacagagaa atatcttttt
    10321 tcttattgat ttctccgcta gttggctcct cttcttcaac taaaatcttc aaaagagtag
    10381 acttacctgc accatttttc ccaacaagag caatccgatc tcgttcatca acctgcaggt
    10441 tgatattatc gaaaagaacc tctcctgcaa aagaacgttc aattttatta gcttgtaaaa
    10501 taatcataag agtagtatag catgtttccc taaggcattc aagataatcg taagt
//
LOCUS       DAVKGU010000069        10439 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKGU010000069 DAVKGU010000000
VERSION     DAVKGU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.069"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(50..1372)
                     /locus_tag="VW077_001581"
     CDS             complement(50..1372)
                     /locus_tag="VW077_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9455576.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1463..1551)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1715..4051)
                     /locus_tag="VW077_001582"
     CDS             complement(1715..4051)
                     /locus_tag="VW077_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9455577.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYTNLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4155..4703)
                     /locus_tag="VW077_001583"
     CDS             complement(4155..4703)
                     /locus_tag="VW077_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9455578.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4700..5002)
                     /gene="zapA"
                     /locus_tag="VW077_001584"
     CDS             complement(4700..5002)
                     /gene="zapA"
                     /locus_tag="VW077_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9455579.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5089..5970
                     /locus_tag="VW077_001585"
     CDS             5089..5970
                     /locus_tag="VW077_001585"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9455580.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5974..6588
                     /gene="lepB"
                     /locus_tag="VW077_001586"
     CDS             5974..6588
                     /gene="lepB"
                     /locus_tag="VW077_001586"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9455581.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6716..9082
                     /locus_tag="VW077_001587"
     CDS             6716..9082
                     /locus_tag="VW077_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9455582.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPGQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNSIPAHDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(9130..10413)
                     /locus_tag="VW077_001588"
     CDS             complement(9130..10413)
                     /locus_tag="VW077_001588"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9455583.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         2948 a         2398 c         2143 g         2950 t
ORIGIN      
        1 gataaaaatt atttataggc aaaaaaatca cacgagctgt gtgattccat tatttgtcaa
       61 aatacttttt agtttcagca ataacgactg gcgacaagac caagagggca atcaagtttg
      121 gcagagccat caaggcgtta acgatatctg cgataatcca gaccatatcc aactcgataa
      181 atcctcctaa caagaccatg agcacaaaaa ccacacggta gagccagata aagcgagccc
      241 caaagaggaa ctcaaaacag cgttctccgt aatagttcca acctagaatc gttgtaaagg
      301 caaaaagtac aaggaagatg gtcaagaggg caggcccaaa gtgtgaaaag actgttgaga
      361 aagctgactg agtcaaggca accccattca agtcaccact ccaaactcca gttaccaaga
      421 tggtcaaacc agttagagta caaatgatga gggtatcaat aaaggttcct gtcatggaaa
      481 tcaaaccttg ctctactggt tcatttgtct tggcagctgc agctgcaata ggagcagaac
      541 ccagaccaga ttcgtttgag aacacaccac gcgccacacc attttgaata gccatccgaa
      601 cgctagcacc agcaaatcca cctaccgcag caaggggact aaaagctgag gtaaagacta
      661 aagcgattgt gccagggatt tttccgatat taaagaaaat aactgtaaga gttcctaaga
      721 tataaatgag ggccataaaa ggaacaacag tagttgaaac cttagaaata gacttgagtc
      781 caccaaagac tgcaatcgct acaaagacag acaagacgag agctgtgatg gctggcgaaa
      841 tcgtcgttgt attttggata gattctgtaa tcgagttgac ttgggtgaag gttccgattc
      901 ccaagagagc aaccaatact cctgctactg caaacaaaac agcaagtggt cgccactttt
      961 ctcccatccc tagaaggata tagtgcatgg gacctcccgc tactgcacca tggtcgtcct
     1021 tggtgcggta tttgatggcc aagagtcctt ccgcatactt ggtagccatt ccaaagaaag
     1081 ccgccatcca catccaaaat agagctcctg gtccaccaac cttgatagcc gtcgcaactc
     1141 ctatgatatt tcctgttcca acagttgatg ccaaggctgt acacagagct gcaaaactgg
     1201 atacatcacc atgtccctta tcctggataa aaataagctg aaaggccttg ggcagacgca
     1261 aaacctgcaa gagtcctagc cgcatggtta ggtaaatccc tgttccgacc aataaaatca
     1321 agaggggcgg tccccaagca aaagcatcga ttgatttaag caattctaac atttccttct
     1381 cctatcgttt caaccccaaa agaaagagca catgcaagat acatgtactc tggaatgctt
     1441 agataaatgc taaaaagcgg tctatcctag ctctgtcctt ttacctgaga gtttgagcag
     1501 ttgcctgcct tgccccttcg gtgcctttac ggtctctcca gagttccgtc catttacagt
     1561 catggaaaat caaacgattc cccacttcta ttaaacttca ttcggtgttg gtatttaatt
     1621 gattctaatt ttacaaaaaa tgttggcttt tgtcaatgtg tttattagta aaaattagtt
     1681 caacagtttt tactttataa agtccagaat actgctatcc tttaaaagtg acaatagtcg
     1741 caccactgcc tccagcattt tgtggggcat agccgaaact cttgacatgt ttgtttcttt
     1801 gcaagtattt ggtaactcct tcacggatga ctcctgttcc gataccatgg atgatatcaa
     1861 cttgagccat attgttaagc aaggcttggt cgatgaaggt atctagctca ttcatggctt
     1921 cttcatagcg cttgcctcga agatccagtc tagcttgagg tcctcgccca gaagttcgtt
     1981 tcacaacatt gacctgtttc ttcttgactg gtttttcttg ctgggcttga acaagatcaa
     2041 actctttctc ttccaaggtc atcttaatca agccaacttg ggcttcccag cgaccgtcct
     2101 tgagttgact ggtcaaggta ccacgctgac cataactgag aaccacgata tcatctccca
     2161 cctttggagc tcgttttttc ttggcctttt gaaggacctt atttttagac aagtccactt
     2221 tttcaggagc caattttttc aacttggcct tggcttcaat gatttcgtgg ggcttgagtt
     2281 gggatttact gtggagattt ttgagaatct ggtcactttc acttagggcc atatccacaa
     2341 tctcagcagc ctgttcacgc gccttgttaa gctcggtttc cttttcacga ttaagctcgt
     2401 tgtagagttt ttttagcgca cggttcatct tgagattttc ttgctccacc tcacggatat
     2461 tgtccaaacg tttgcggctt tccagcgtct gctcttctaa ttgctcaatg atacgattga
     2521 cgtcattgtc ctgatcgatc tgctgactgg catctcctac gataacttca gataggccta
     2581 gacgtttggc aatttcaaag gcattacttc ggccaggaac accctgcata aagcgatagg
     2641 tcgggcgaag agttgcagta tcaaactcca tactggcatt ttgcacaaag gctgtctcaa
     2701 taccgtaggc cttgagttct ggatagtggg tcgtcgccat ggtcttgatt tgacgcaggc
     2761 gaaggtcctc cagaatagcc atggcaaggg ctgctccctc ttggggatca gtaccagccc
     2821 ccaactcatc caaaagtaag agtgaatgtt ggttgacctt gccaagaata tccacgatat
     2881 tggtcatatg gctagagaag gtagacaagc tctgctcaat agactgctca tctccaatat
     2941 cagcaaagat ttcttcaaaa ataccaacac gacttccctt gtctgctaaa atcggcaatc
     3001 ctgactgggc catgacctgt gtcaagccca gagttttgag catgatggtc ttcccacctg
     3061 tattgggacc tgtaatgaca atagctgtta aatcttgacc aaaatggaca tcatttgcga
     3121 cggcattttt gaccaaagga tggcagacat ggagcagttg aatctcttga ttttctgaca
     3181 gctgaggcac gactgcttgt ctttcttgga taaatcgaac cttggcacga atcaagtcca
     3241 gatgaccgat aatccaagcg tcattagcaa tctcagccgc atgagggcgg acacgctcag
     3301 aaatttcttg gagaatgcga agcatttcat agcgctcatc tgctcgcaga ctagcaattt
     3361 cttcgctcag tttgactacc tcacgtggtt cgatatagac ggtgtttcca ctagcagaaa
     3421 tatcatgaac gacacctgca atcttattgc ggtaggtgtt tttgactggt aaaacctgac
     3481 ggccatttct gctagcaaca attccttccg tcaacatctg cgctttttgc ttgagcaagt
     3541 cttgtaaaac atcgcgtacc tgactctcgc tatcatgtat ttttcgacgg attcgcgcca
     3601 attcttcact ggcaaaattt tcaatgaaac ccgcatcatt aaaggcctga agatttcctt
     3661 gtaattgcgg aaaatcatgt aatttctcaa accaaagggc taattcttcc aagctgacat
     3721 tttccagatt ggtgtaaaaa ttttgaagtt ctcggctggc aagaagcacg cgtttcaaga
     3781 gtaggaactc ctcgatattg agatccgctc ccatctccaa cctcttgcag actcctgcaa
     3841 tttccttagt tgagagaata gtaaaatgcg gttgctcgac gaaaagagcc tgcatttcct
     3901 tcatctcagc aaaagcctgt ttgattttat ctgctttggc agtcggagcc agttgtctca
     3961 attgctccaa gccctgctcg gtcaacaaat gaggctcaaa caaggccttg accttatcga
     4021 actctaatgt ttctaatatt ttcttattca tctttatttc cttgtctttg aatgtctagg
     4081 tgtggtagct ttttacattc tgatagattc gagtcaaatc tgtaaacaaa gggctaggaa
     4141 aactcctgcc ctttttatcc gattaaattt gtcacccaga tttgtttgag ccaactggtt
     4201 gttatcggta tgctctggat gatgtgtttt gcgacgatac tcttttcaag aggattttgt
     4261 ataactgcca tggggatggt cgccaagatt gtcaaggcca tttgcaagac aaataaggtc
     4321 accaacatgg acaagatacc tgctgaaact tggaacaact taccacccag ttttttgcta
     4381 ggaatcaagt gtaagagaag accaagcaaa cgaccaatgc tatagacaat cccaaataca
     4441 agcaagtagc cgatacctgc ataaaagacc ttatccagct gaaagagttg atccgatggg
     4501 aaaaagaaag tcccctgacc ttcctgcgaa tttgcataag ggagcaataa atggaattgc
     4561 tctccaagcc ccttataaaa ctggccagcc ataaaagccg atgccatggc tgaaatcagg
     4621 taataaacct gtaagagcag gcctctccga tagccgatat aaaatcccca aaccaagacc
     4681 aatagaagaa ggaatgaaat cataaggaat cctcaatctt gctctgttct tgcttacaag
     4741 tcacaagctt gtgacggagt tcttctagct cttgctcctt atcgtcaaat tcaatctcac
     4801 ggctgagctg agttgataaa cagttgactg ccaacaaaag agcgattgtt tcatcatctg
     4861 cgctaggcat ttgttcttta attgcttggt atttttctgt cgcaacctta gcgatttcct
     4921 ccataaaaag gttatcatgt tcgcttgtca aggttaacga ttttttcccg aatgtaaatt
     4981 tgaatcgatt tagatttgcc ataaaattca cctcacgata ttataccaaa attcgctaat
     5041 tttgtcagtt tttacaaatt ttactgcttt tgtggtacaa tagaaactat ggcaagtatc
     5101 acactcacac caagcgaaaa ggatattcag gcttttcttg aacactatca aaccagtctg
     5161 gctcccagta agaatcccta tatccgctac tttttgaaac tacctcaagc aacggtttct
     5221 atctatactt ctggaaaaat cttgcttcag ggtgaagggg ctgagaaata cgctagtttc
     5281 tttggctatc aagctgtaga gcaaaccagc ggacaaaatc ttcctttaat tgggacagat
     5341 gaggtgggaa atggttccta ctttggtggg cttgcagttg tggctgcctt tgtcacatct
     5401 gaccagcacg actttttacg aaaactcggt gtgggggatt ctaagactct gaccgaccaa
     5461 aagatccgtc agattgctcc tattctcaag gaaaaaatcc agcaccaggc actccttctc
     5521 tcacccagca agtacaacga ggtcatcgga gaccgctaca atgctgtttc ggttaaggtt
     5581 gccctccata atcaggctat ctatctcctc cttcaaaaag gtgttcagcc tgagaaaatt
     5641 gtgattgatg cctttaccag tgctaaaaat tatgacaagt acttggcaca agaggccaat
     5701 cgtttcagca atcctatcag cttagaagaa aaggctgagg gcaaatactt gtctgtcgca
     5761 gtttcttctg tcattgcgcg tgatctcttt ctggaaaatc ttgaaaattt gggacgagaa
     5821 ctgggttatc agctcccaag tggagctgga acggcttctg acaaggtggc tagccagatt
     5881 ttgcaagcct atggtatgca gggactcagc ttctgcgcca aattgcactt taaaaacact
     5941 gaaaaagcga aaaaacgctt agaaaggtaa gttatgaatt catttaaaaa tttcttaaaa
     6001 gagtggggat tgttcctcct aattctgtca ttactagctt taagtcgtat ctttttttgg
     6061 agcaatgttc gcgtagaagg acattccatg gatccgaccc tagcggatgg cgaaattctc
     6121 ttcgttgtaa aacaccttcc tattgaccgt tttgatatcg tggtggccca tgaggaagat
     6181 ggcaataagg acatcgtcaa gcgcgtgatt ggaatgcctg gcgacaccat tcgttacgaa
     6241 aatgataaac tctacatcaa tgacaaagaa acggacgagc cttatctagc agactatatc
     6301 aaacgcttca aggatgacaa actccaaagc acttactcag gcaagggctt tgaaggaaat
     6361 aaaggaactt tctttagaag tatcgctcaa aaagcccaag ccttcacagt tgatgtcaac
     6421 tacaacacca actttagctt tactgttcca gaaggagaat accttctcct cggagatgac
     6481 cgcttggttt cgagcgacag ccgccacgta ggtaccttca aagcaaaaga tatcacaggg
     6541 gaagctaaat tccgcttctg gccaatcacc cgtatcggaa cattttaaga aacctaagag
     6601 gccgagaatc accaatctca gcctcttctt ctatcgtgag aaaatgattg gtaactatct
     6661 aaacttacca gaacagaaac acctcaactc tcacctattc atgcaaagga attttatgga
     6721 agtttatttt tcaggaacca ttgaacggat tatttttgaa aatcccagca atttttatcg
     6781 catcctcctc ctagaaatcg acgatacgga cgcagaggat tttgatgatt ttgaaatcat
     6841 tgtcacagga accatggctg atgtaattga gggcgaagac tatacttttt gggggcaaat
     6901 tgtccagcac tccaagtatg gagaacaact gcaaatcagt cgttatgatc gcgcaaaacc
     6961 aactagtaag ggcttggtca agtacttttc aagtagccat ttcaagggga ttggtctcaa
     7021 gacagctcag aaaatcgtgg atacctatgg cgaaaatacc attgacgaaa ttttgcaaca
     7081 cccagaaaag ttagaaggca tcgcaggact ctctgccaaa aatcgcgagg ctttcgtctc
     7141 cactctccgt ctcaactacg gaacggagat ggttttgtcc aaactagcca actacggcat
     7201 tcccaacaaa ctagcctttc agattcaaga cttttacaag gaagaaaccc ttgatgtggt
     7261 tgaaaattat ccctaccagc tggttgagga tatcaagggt ttgggcttta ccattgctga
     7321 ccaactagct gaggaactag gcatcgaaag tcaggctcct gaacgcttcc gcgccggtct
     7381 agttcacagt ctttttcagg cctgtatgga aacaggggac acctatgttg aagcacggga
     7441 tttgctggaa caaaccctta ctctccttga gtcttcccgt cccgtggaac tagaccccgg
     7501 ccaagttgcc caagagctct cctacctgat cgaagaagac aaggttcagc agattgatac
     7561 caagatcttt gacaacagcc tctttttcgc tgaggaaggt atccgcagtc acttgattcg
     7621 tatccttgaa aaaggaaaac agaagagcca agatttagaa actattcaaa agcatatcac
     7681 tactgtcgag caagaactgg ggattgagta tgataacatt caaaaacagg ctatctgtga
     7741 tgctatccag aataaggtct ttatcctgac aggtggacct ggtactggta agacaactgt
     7801 tatcaatggg atcatcgctg tttacgccct gttagaggga cttgacctca ggaagaaaag
     7861 caacctgccc attcttcttg ctgctccaac tggtcgagct gctcgtcgca tgaatgaatt
     7921 gacaggtttg cctagcgcga ccatacaccg ccacttggga atgacaggtg acgatgatac
     7981 cagtcatctg gaagattacc tagatgctga ctttatcatc gtggatgaat tctctatggt
     8041 ggatacttgg ctggccaatc aactcttctc caatatctct tctaacagta agatcctcat
     8101 cgtaggtgac agtgatcagc taccttctgt cagtcctgga caggttctag cggatctgct
     8161 tcatattcct ttgattcctc agactcgctt ggaaaaaatt tatcgacaaa gcgaagaatc
     8221 aaccatcgtc accctagcta gtcagattcg acagggcatc ttgccagctg atttcaccca
     8281 aaaaaaagct gaccgttcct actttgaaat tgctagtggc catattcctg ccactattga
     8341 aaaaatctta ggtgccgccc tcagaaatag tattcctgcc catgatatcc aagttctggc
     8401 tcctatgtac cgagggacgg cagggattga tgctatcaat cagctcatgc aagacctgct
     8461 caatccccca caaaaagatc aactcagttt tgaagctcct cagtgccact atcgtaaggg
     8521 agacaaggtc attcatttgg ttaacgatgc tgaaatcaat gtctttaatg gggatttagg
     8581 agctatcaca gacctgattc ctggtaaata caccgaatcg aaacaagacg agattgtcat
     8641 tgattttgat ggtaatgaag tctcttaccc ccgtaacgaa tggtacaaga ttcgcttggc
     8701 ctatgccatg agtattcata agtctcaggg aagtgagttc ccagttgtca tcctaccgat
     8761 tactagtgct agccgacgta tgctggagcg aaatctcatc tacacagcca ttacacgtgc
     8821 caaaagcaaa ctcatcttac taggcgaatt acaagccttc gactatgcta cccaacacat
     8881 cggaactgcc cgaaaaacct atctgattga acgcttcagt gatctactgg agaatgttga
     8941 agaaaagcaa caagctgtct ctgaaacagt cacatcaagt gcctctgaac aatcctacat
     9001 cctaaccgaa gaaaactggg accgcatccc agccatgatt gggattacag acacagacct
     9061 caaagagatt tttggaaaat agtgcatcag aaaacccacc aattcaggtg ggttttctgt
     9121 ttattaagat tattttactg ttgctgtgct tgtgatcaat tcaacagctt ttttgatagt
     9181 gatatcatgt ttcaacatgt cagctgaaag caagttttga acttgtgcaa cttccatgtt
     9241 gtagtctgct gccaattgct caacttcttt ttggatttct tcttctgaag catcaaatcc
     9301 ttcagctttg gcaactgctt cgataacaag gttagtctta gtacgtgact cagcttctgc
     9361 ttggtattgg ttgtgaaggt cttcttgagt agttccagtg atttggaagt acatgtcagg
     9421 gttgatccct tgacgttgca aattcccaag gaattcattt actgaacggt gaacttcttc
     9481 atggatcatt tcttctggaa gttctacgat ttcagcattt tctacagctg tatcaattgc
     9541 tgcaccttca actgcatctt tgtaagcttc ttctttagca gcagccaatt ctttgcggta
     9601 tttttctttc aagtcagcaa gtgtttcaac ttcttcatca atgtcttttg caagttcatc
     9661 gtcaagagct ggaacttctt tagcttttac ttcgtggata gttgtcacga atttagcttc
     9721 tttacctgca aggtcttctg cttggtagtc ttctgggaat gttacgataa catcaacggt
     9781 ttcgccagct gagtgaccta ccaattggtc ttcgaaacca gggatgaatt gacctgaacc
     9841 aagtccaagt gagaagtttt caccttttcc gccgtcaaat tcaacaccgt cgatagaacc
     9901 aacgaagtcg atcacaacag tgtcgccgtt ttcagcagca gcttccttga taaccaattc
     9961 agccaggttg ttgcgttcgc gttcgatacg ctcttcaaca tcagcgtcag ttacttcttt
    10021 ttctacatca actgatactt caaggttttt gtagtcaccc aattttactt caggttttgt
    10081 aacgacttca gcagcgataa cccagtcttg acctttttcc attgaagtta cgtcaatttt
    10141 tggttgggca accacttcaa gaccagcttc ttttacagct gcttcataag cgtttggcaa
    10201 aagagcgttc ataacgtctt ggtaaagtga ttcttcacca aattttttat cgaagatagg
    10261 acgtggaagg tgacctttac ggaaacctgg aacattaaga gatttcttca ctgacttgaa
    10321 gacacggtcc aattctggtt tgatttggtc ttgagagata gtgaaagtca agacaccacg
    10381 gtttgtttct ttgttttcaa atgatacaga cattctgtca tttctcctta aaatttttt
//
LOCUS       DAVKGU010000070        10380 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKGU010000070 DAVKGU010000000
VERSION     DAVKGU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.070"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..185
                     /locus_tag="VW077_001589"
     CDS             <1..185
                     /locus_tag="VW077_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9455584.1"
                     /translation="EYENRTVSFAISSKGEKMKVENISYRVDHRKLFDNISFDTSSSD
                     VTLITGKNGTGKSTLL"
     gene            complement(551..1861)
                     /locus_tag="VW077_001590"
     CDS             complement(551..1861)
                     /locus_tag="VW077_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9455585.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2098..2970
                     /locus_tag="VW077_001591"
     CDS             2098..2970
                     /locus_tag="VW077_001591"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9455586.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3101..3947
                     /locus_tag="VW077_001592"
                     /pseudo
     CDS             3101..3947
                     /locus_tag="VW077_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            4008..4970
                     /locus_tag="VW077_001593"
     CDS             4008..4970
                     /locus_tag="VW077_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9455587.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPIESLCYE"
     gene            5147..6154
                     /gene="gap"
                     /locus_tag="VW077_001594"
     CDS             5147..6154
                     /gene="gap"
                     /locus_tag="VW077_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9455588.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(6222..7058)
                     /locus_tag="VW077_001595"
     CDS             complement(6222..7058)
                     /locus_tag="VW077_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9455589.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWKEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            7184..9379
                     /locus_tag="VW077_001596"
     CDS             7184..9379
                     /locus_tag="VW077_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9455590.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETKAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEASWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            9432..9584
                     /gene="rpmG"
                     /locus_tag="VW077_001597"
     CDS             9432..9584
                     /gene="rpmG"
                     /locus_tag="VW077_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9455591.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNLKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            9594..9770
                     /gene="secE"
                     /locus_tag="VW077_001598"
     CDS             9594..9770
                     /gene="secE"
                     /locus_tag="VW077_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9455592.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            9825..10361
                     /gene="nusG"
                     /locus_tag="VW077_001599"
     CDS             9825..10361
                     /gene="nusG"
                     /locus_tag="VW077_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9455593.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         3249 a         1802 c         2257 g         3072 t
ORIGIN      
        1 aagagtatga aaacagaact gtttcttttg ctattagttc aaaaggagaa aaaatgaaag
       61 tagaaaatat ttcgtatagg gtggatcatc gtaaattgtt tgataatatt tcttttgata
      121 cttcgagttc agacgtgaca ttaattactg gtaaaaatgg tacaggaaag tcaactttac
      181 tatagtagat tgaaactaga atagtacaca tctacttcta aaatattgtt agaaatcgat
      241 ttgactatcc tgatctattt gtcctgttct tatttcattt cactatatct caaattgagt
      301 atgacgaagt gcgctcccat gtcctgggaa cgcactttct tcatattttt catattcttg
      361 aatccatcga taaagactat tgggatgaat ttttaaagtt gaactaatca tttttacagg
      421 atgagattta cagcagagag tttgaaggct ttatcaaagg tttttcttgg cataatgact
      481 tttcctcgtt tccacttaat tttgtgtcta ctttattata ccaagtccac ccttaagtta
      541 gataataaat ctaacttaag gaagctagaa ggatgagaat ccaggtggtc aagagtccca
      601 aacttaagct gatggggaca cccagaataa tttgcttttt gaaggcaagg ccacgttcct
      661 ctatattggg aagtgagagt tgaatgagag aaccagctga tgaaaagggt gagatattag
      721 tagatagagc gccaataacg gtggctgttg tgagtaagtg aatatcaatc tgaggatttt
      781 gagcactgat gatagcaatg atgggaaaga gggctggagc tacaacggat agggtggaac
      841 taaagagtga catcactccg gctatcacac aaaagaacag aggtaaccag aaatgaggaa
      901 tggttgttgt catgaggtgc cctatcagtg tgactaaacc tgacttgacc gctagagaca
      961 ttagtaagct catgccgcag agcatgataa ttgtagccca gggaacctta gctaaaatgg
     1021 cttcttgctt ccctaatttg agccttaagg cgaggcagac catgagtatt gagacaaagc
     1081 caatatcaaa tgttttttga taagtagcta tccaggcgat gtttgggaaa atgagatgca
     1141 acaagggaaa aagccaaacc aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt
     1201 gaaccttgct gaggagtggt ggttggtcaa tagtcaagga tgagtttgtt cttcccttac
     1261 tatagtgact gtaacaggat aataaaagca agacgatgag tgggtagata atgctgacga
     1321 taaagatatg attgccaagt gaaaaagctt gctcttccca tcccatttgc ttaaacaggc
     1381 cttgaaagac aatgcctgag ctactggtta tcaaattagc ccctcctgaa gctccccaat
     1441 tgacggcttg agctccaatc aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta
     1501 gaggacagca aacggccata gtagtgaaaa atccagcacc taaagcagac aaaagggttg
     1561 ccatcaggta taaaatcatg tagagggcgt tagggtgggt gcgtgtgcgg tagagaatgt
     1621 gttgagccaa aacatcaaga gtaccgttag ttgttgcaac gttataaaag agagagacgc
     1681 taaaaatggt aaaaaagagt gaggttggcc aaaaatgaag aagttctttg gggcttaatc
     1741 ccatgagagt ggttgcgatg aggtaagaaa aagcaatagc cagcaggcca atattgattt
     1801 tggtgcggta accaattcca atggctagag caatggcgct aatcattatt aaatgaatca
     1861 ttgaattgtc ctttcagtta gaatatggaa ataggttggc aattttggcg ctcaaaagac
     1921 cacctgtttc ctattggtac ttgactatta tacacaaaaa aaatacaatt gtcttgacaa
     1981 ttgtattgac acttgttaga attaaaaatg aggtattcag gcttgtgatt tcctatggaa
     2041 gttaatagtg attgcctcta atgcttacaa gtgatattaa aaatagagga cctagtgatg
     2101 tcaatcattt caactgattt aacccctttt caaatagatg atacattgaa agcagccttg
     2161 cgagaagatg ttcattccga agattacagt accaatgcca tttttgatca tcatggccaa
     2221 gccaaggtgt cgctttttgc caaggaagct ggtgttttag cggggctaac cgtttttcaa
     2281 agggttttta ccctatttga tgccgaggtg accttccaga atcctcatca atttaaggat
     2341 ggggatcgtt tgactagtgg cgatttggtt ttagaaatca taggctcggt gagaagtctc
     2401 ttaacatgtg aacgcgttgc cttgaatttt ttacaacatt tatcagggat tgcttcgatg
     2461 acagctgctt atgtagaagc cttaggcgat gattgcatta aggtatttga tactcgaaaa
     2521 actactccta atttacgtct ttttgagaaa tatgccgtga gagttggcgg tggctataat
     2581 catcgcttta atttatcaga tgctatcctg ctaaaagaca atcacattgc ggcagtaggt
     2641 agtgttcaaa aggcaattgc tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag
     2701 gtggaagtgg aaagccttgc tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc
     2761 atgttggata atatgtcatt ggaacagatt gaacaggcca ttaccctaat tgcaggacgt
     2821 tctcggattg aatgttctgg aaatattgat atgaccacta ttagccgttt tcgtggttta
     2881 gcgattgatt acgtctccag tggtagttta acccatagtg ctaagagtct tgatttttcc
     2941 atgaaggggt taacctacct tgatgtctaa gttgtaaaat aaactaactt tttaaaggat
     3001 gtctttcctc tagaacgagt tttatgtcag atagtttaaa cgcctcttca aatatagtaa
     3061 aatgaaccaa aaatagtaca caatgtggta taatcttttt atggcatatt caatagattt
     3121 tcgtaaaaaa gttctctctt attgtgagcg aacaggtagt ataacagaag catcacacgt
     3181 tttccaaatc tcacgtaata ccatttatgg ctggttaaag ctaaaagaga aaacaggaga
     3241 gctaaaccac caagtaaaag gaataaaacc aagaaaggtt gatagagata gacttaaaaa
     3301 ctatcttact gacaatccag acgcttattt gactgaaata gcttctgaat ttggctgtca
     3361 tccaactacc atccactatg cgctcaaagc tatgggctac actcgaaaaa aagaaccaca
     3421 cctactatga acaagaccca gaaaaagtag ccttatttct taagaatttt aatagtttaa
     3481 agcacctaac acctgtttag attgacgaaa caggattcga tacttatttt tatcgagaat
     3541 atggtcgctc attaaaaggt cagttaataa gaggcaaagt atctggaaga agatatcaga
     3601 ggatttcttt ggttgcaggt ctaacaaatg gtgagttaat cgctccaatg acttacgaag
     3661 agatgatgac gagcgacttt tttgaagtat ggtttcagaa gtttttctta ccaacattaa
     3721 ccacaccatc ggttattatt atggataatg caagattcca tagaatgggt aagctagaac
     3781 tcttgtgtga agagtttggg cataaacttt tacctcttcc tccctactca cctgagtaca
     3841 atcctattga gaaaacatgg gctcatatca aaaagcacct caaaaaggta ttaccaagtt
     3901 gcaatacctt ttacgaggct tttttatcct gctcttgttt caattgacta tatatcacgg
     3961 atacagttgg gaaagaagct attaaatatt tggagagtcc ttttgggatg aacatgtcaa
     4021 atattactat agatgggcaa gaaatggatc tgagtcaaac gatgccttct tatgctcaag
     4081 tagtgagtct ttatgatgat acaagtatct ctgttagtaa taatgagacg gacaaagtgt
     4141 tggctggatc cctctatact gatacaaatg aacagggatt aacgattcca agcagtttac
     4201 taaaaaattg gaatgaacag acaggaaaaa atttgacagc taatgatctt attggcaaat
     4261 cagtctcagc cagcattgta gaaagtgctg ccgaaactag taagattgct caatttcaaa
     4321 cgaagattgt acgtgtaatc aatgatgaag atgacatgga ggacagcaac agtttcatgc
     4381 tgtctcatca aatggaaacg attttgaaag aggctggatt tacgaaagct gtatcttatt
     4441 ttatcttgga actcaaagat ccatcacaga caaaagtagt aacagaagaa ttacagaaaa
     4501 ataagaagta tactgtgctt tctcaacaga gagttcttga tattgtgatt acctttattc
     4561 gtgttattca gggattattg attgtgcttt catcacaagc tattgtggta gcagcggtta
     4621 tgattggtat cattatttac atcaatatca tgcaacgttc caaggaaata ggtgtcatga
     4681 aagcagttgg ttatcagaat cgtgatgtca aaggaatttt tatttacgag gctatctgga
     4741 ttgtaggcat cgccttgctg ctggcatttt tggttgcaca aggggtggga agtttggcga
     4801 atgcgattgt aagtcacttt tacccatcca tcactaaggt ttttgaatta aatcttttat
     4861 ctgttttagg aactctagtt ttcgctctat tacttggtta tgtctcagcc tacttcccgg
     4921 cgcgtaagat tagtaaaatg gatcctatag aatcgttatg ctatgaatag taagggattc
     4981 cttggtttac agtcttaaaa agaaactaac atagaaaaac aatggccata cttgcaattc
     5041 aactcaaata gttgtataat aggtttgttg aaaggtgatt tgtagtgatt caagttactc
     5101 ttttcacaat aaaaatttca tgattttcat aaggaggaaa tcactaatgg tagttaaagt
     5161 tggtattaac ggtttcggac gtatcggtcg tcttgctttc cgtcgtatcc aaaacgtaga
     5221 aggtgttgaa gttacacgca tcaacgacct tacagatcca gttatgcttg cacacttgtt
     5281 gaaatacgac acaactcaag gtcgtttcga cggtactgtt gaagttaaag aaggtggatt
     5341 tgaagttaac ggtaaattca tcaaagtttc tgctgaacgt gatccagaac aaatcgactg
     5401 ggctactgac ggtgtagaaa tcgttcttga agctactggt ttctttgcta agaaagaagc
     5461 agctgaaaaa caccttaaag gtggagctaa aaaagttgtt atcactgctc ctggtggaaa
     5521 cgacgttaaa acagttgtat tcaacactaa ccacgacgtt cttgacggta ctgaaacagt
     5581 tatctcaggt gcttcatgta ctacaaactg cttggctcca atggctaaag ctcttcaaga
     5641 caactttggt gttgttgaag gattgatgac tactatccac gcttacactg gtgaccaaat
     5701 gatccttgac ggaccacacc gtggtggtga ccttcgccgt gctcgcgctg gtgctgcaaa
     5761 catcgttcct aactcaactg gtgctgcaaa agctatcggt cttgtaatcc cagaattgaa
     5821 tggtaaactt gacggatctg cacaacgcgt tccaactcca actggatcag ttactgaatt
     5881 ggtagcagtt cttgaaaaga acgttactgt tgatgaagtg aacgcagcta tgaaagcagc
     5941 ttcaaacgaa tcatacggtt acacagaaga tccaatcgta tcttcagata tcgtaggtat
     6001 gtcttacggt tcattgtttg acgcaactca aactaaagtt cttgacgttg acggtaaaca
     6061 attggttaaa gttgtatcat ggtacgacaa cgaaatgtca tacactgcac aacttgttcg
     6121 tactcttgaa tacttcgcaa aaattgctaa ataattcttg agttgataga aagcaaggct
     6181 ttgtggtctt gcttttttat atggaaaaat ggatgacacg atcatccatt cttttttaat
     6241 tctttttcaa atgtatttga aagggtagtg aaagttagct tctctaaagt aagtgggtgg
     6301 gtaaaggaaa gtcggaaggc atgaagcata agccggcttg tctttgattt actattatag
     6361 agagggtctc ccaggatagg aagattatga tgcgaaaggt gcacacgaat ctgatgggtt
     6421 cgccctgtct ttagcttgca atgagccaag gaagtcttgt ttgagaattg ctttaatctg
     6481 cttacatgcg tttcagcata ttgcccattt tttgcatcaa ctattctttt tctacgatca
     6541 tggcgatcac gtccaatttt gtctctgaaa acaagttctt ttctgttgat atttccatca
     6601 actagagccc aatattctct agaaatctct tttttctcca ataagcgatt gagaatgggc
     6661 aggataaaag gatttttggc aaagagaact aagccactgg tttccatgtc cagacgatga
     6721 acgacatagc aggtttggcc aacataggta ctgacatggt taagaagggc aatttcgttt
     6781 ggttgattac catgcgtttt catcccctct ggtttgttta caataatcaa gtgttgatct
     6841 tgataaactt cctgcactaa gtctgggttg ccccaaggga tcgtcttttg ggaataatct
     6901 tcctcgtcaa aagtcaactg gcaaacatct ccaggattta cgatttcctt ccagtggact
     6961 tcttcttgat ttatcaaaat atgtttcttg attctcaaaa aatgacggat ttttctaggg
     7021 atgaggagtt gttcctcaag taattgcttt actgtcattt gaggtagaga ggcgggtaat
     7081 gtaaatgtga attgcataca gatattgtaa caaaaaaagc cctatttgga taggaaatag
     7141 ctaaattctt gtcttcctat gatgaagatg ataaaataaa cgcatgaaat tagataaatt
     7201 atttgagaaa tttctttctc tttttaaaaa agaaacaagt gaactagagg actctgattc
     7261 tactatctta cgtcgctctc gtagtgatcg aaaaaaatta gcccaagtag gtccgattcg
     7321 aaaattctgg cgtcgttatc atctaacaaa gattatcctt atactaggtt tgagtgcagg
     7381 cttgctagtt ggaatctatt tgtttgctgt agccaagtcg accaatgtca atgatttgca
     7441 aaatgccttg aaaactcgga ctcttatttt tgaccgtgaa gaaaaagagg ctggtgcctt
     7501 gtctggtcaa aagggaacct atgttgagct gactgacatc agtaaaaact tgcagaatgc
     7561 tgttattgcg acagaagacc gttctttcta taaaaatgac gggattaact atggccgttt
     7621 tttcttggct attgtcactg ctggacgttc aggtggtggc tctaccatta cccaacagct
     7681 ggctaaaaac gcctatttat cgcaggatca aactgttgag agaaaagcga aagaattttt
     7741 ccttgcctta gaattaagca aaaaatatag taaggagcaa attctaacca tgtaccttaa
     7801 caacgcttat tttggaaatg gtgtgtgggg tgtagaagat gcgagtaaga aatactttgg
     7861 agtttctgca tcagaagtga gtctggatca agctgcgact ctggcaggga tgctcaaggg
     7921 gccggaactg tataatccct tgaattccgt agaagattct actaatcggc gcgatactgt
     7981 cttgcagaat atggttgcag caggatatat tgataaaaac caagaaacca aagctgctga
     8041 agttgatatg acttcgcaat tgcacgataa gtatgaagga aaaatctcag attaccgtta
     8101 cccctcttat tttgatgcgg tggttaatga agctgtttcc aagtataatc taacagagga
     8161 agagattgtc aataatggct accgcattta cacagagctg gaccaaaact accaagcaaa
     8221 tatgcagatt gtttatgaaa acacatcgct atttccgagg gcagaggatg gaacgtttgc
     8281 tcaatcagga agtgtagccc tcgaaccgaa aacaggggga gttcgtggag ttgtcggtca
     8341 agttgctgac aatgataaaa ctggattccg gaatttcaac tatgcaaccc aatcaaagcg
     8401 tagtcctggt tctacaatta agcctttagt tgtttatacg ccagcagttg aagcaagctg
     8461 ggctttgaat aagcagttgg ataaccatac catgcagtat gacagctata aggttgataa
     8521 ctatgcaggg atcaaaacga gtcgagaagt tcctatgtat caagccttgg cagaatcgct
     8581 taatctacct gctgttgcca ctgttaatga tttgggtgtc gacaaggctt ttgaggcagg
     8641 cgaaaaattc ggactcaaca tggaaaaggt cgaccgtgtt cttggtgtcg ccttgggaag
     8701 cggtgttgaa accaaccctc ttcaaatggc tcaagcatac gccgcctttg caaatgaagg
     8761 tttaatgcct gaagctcatt ttattagtag aattgaaaat gctagtggac aagttattgc
     8821 gagtcataaa aattcacaaa aacgggtgat tgataagtct gtagctgaca agatgaccag
     8881 tatgatgttg gggactttca ccaacggtac cggtattagt tcatcgcctg cagactatgt
     8941 catggcaggg aaaactggaa caactgaagc agttttcaat ccggagtaca caagtgacca
     9001 gtgggtaatt ggttatactc cggatgtagt gattagccac tggcttggct ttccgaccac
     9061 tgatgaaaac cactatctag ctggctctac ttcaaacggt gcagctcatg tctttagaaa
     9121 cattgccaat accattttac cttatacgcc aggaagtacc tttacggttg aaaatgctta
     9181 taagcaaaat ggaattgcac cagccaatac aaaaagacaa gtacaaacca atgataatag
     9241 ccagacagat gataatttgt ctgatattcg agggcgtgcg caaagtctag tagatgaggc
     9301 tagccgggct atctcagatg cgaagattaa ggaaaaggct caaacaatat gggattcgat
     9361 agtcaatcta tttcgctaag atgcttgtca aagcctagct ttcttgttat aatggataag
     9421 atggaggcgt tatggcacta aaaaaagcaa gcctagcttg tgcggtttgt ggttcgagaa
     9481 actattcaat caagatcagc ggaaacctca agcctacacg actagaagta aataaatttt
     9541 gtaagcattg tggcaagtac actacacaca gagaaacgag ataggagaga gcgatgcgtt
     9601 ttattggaga tatttttaga cttcttaaag acacaacatg gccaactcgc aaggaaagct
     9661 ggagagattt tcgttctatc atggaataca cagctttctt tgtagtaatt atttacattt
     9721 ttgaccagtt gattgtttca ggtttgattc gatttattaa cattttttag aagattagtg
     9781 gagttaatta cactagaaat cttctattta tgaaaggaaa tatcatggat agttttgata
     9841 aagggtggtt tgttttacaa acttattctg gttatgaaaa taaggtgaaa gaaaatctat
     9901 tacaacgtgc acaaacctac aatatgttgg ataatattct acgcgttgaa attccaacac
     9961 aaacagtgca agttgaaaaa aatggaaaga gaaaagaagt agaagaaaat cgctttccag
    10021 gttatgttct tgtagaaatg gtcatgacag atgaagcttg gtttgttgtt cgaaacacac
    10081 caaatgttac aggatttgtc ggatcacacg ggaacagatc aaaaccaact ccattattgg
    10141 aacaagaaat tcgcgacatt ttggtatcta tgggacaaac tgttcaagaa tttgatttcg
    10201 atgttgagat tggtcaaact gtacgtatta ttgatggtgc ttttgcagac tacactggta
    10261 agattacaga aattgataat aacaaagtga aaatgattat ctctatgttt ggtaatgaca
    10321 cagttgcaga agtaaatcta aaccaaatcg cagaattata accctgagag aggctggagc
//
LOCUS       DAVKGU010000071        10215 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKGU010000071 DAVKGU010000000
VERSION     DAVKGU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.071"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..203
                     /locus_tag="VW077_001600"
     CDS             <1..203
                     /locus_tag="VW077_001600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455594.1"
                     /translation="CFEVADRTDEVSSKHCFEVADRTDEVSSKHCFEVADRTDEVSNH
                     TYDKATLTRFEEIFEEYKGVPR"
     gene            575..1936
                     /gene="trpE"
                     /locus_tag="VW077_001601"
     CDS             575..1936
                     /gene="trpE"
                     /locus_tag="VW077_001601"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9455595.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFYILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLSSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1933..2499
                     /locus_tag="VW077_001602"
     CDS             1933..2499
                     /locus_tag="VW077_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9455596.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            2510..3514
                     /gene="trpD"
                     /locus_tag="VW077_001603"
     CDS             2510..3514
                     /gene="trpD"
                     /locus_tag="VW077_001603"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9455597.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3511..4278
                     /gene="trpC"
                     /locus_tag="VW077_001604"
     CDS             3511..4278
                     /gene="trpC"
                     /locus_tag="VW077_001604"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9455598.1"
                     /translation="MSQEFLARILEQKVREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            4265..4864
                     /locus_tag="VW077_001605"
     CDS             4265..4864
                     /locus_tag="VW077_001605"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9455599.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEVAE
                     LAKLIPADVKKVGVFVSPSRAELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            4842..6065
                     /gene="trpB"
                     /locus_tag="VW077_001606"
     CDS             4842..6065
                     /gene="trpB"
                     /locus_tag="VW077_001606"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9455600.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGSPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            6058..6834
                     /locus_tag="VW077_001607"
     CDS             6058..6834
                     /locus_tag="VW077_001607"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9455601.1"
                     /translation="MPKTLTEKLNAIKVTGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(6806..6907)
                     /locus_tag="VW077_001608"
                     /pseudo
     CDS             complement(6806..6907)
                     /locus_tag="VW077_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            7136..7867
                     /locus_tag="VW077_001609"
     CDS             7136..7867
                     /locus_tag="VW077_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455602.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDENSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            7882..9033
                     /locus_tag="VW077_001610"
     CDS             7882..9033
                     /locus_tag="VW077_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9455603.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(9085..9744)
                     /locus_tag="VW077_001611"
     CDS             complement(9085..9744)
                     /locus_tag="VW077_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9455604.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEKSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            9814..>10215
                     /locus_tag="VW077_001612"
     CDS             9814..>10215
                     /locus_tag="VW077_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455605.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQM"
BASE COUNT         3191 a         1861 c         2406 g         2757 t
ORIGIN      
        1 actgttttga ggttgcagat agaactgacg aagtcagctc aaaacactgt tttgaggttg
       61 cagatagaac tgacgaagtc agctcaaaac actgttttga ggttgcagat agaactgacg
      121 aagtcagtaa ccatacctac gataaggcga cgttgacgcg gtttgaagag attttcgaag
      181 agtataaagg tgtaccgaga tgaacatctc ttcatcaatg tctctggtcg tggaggcaag
      241 gacctagctg cgattgcaga ttactagaag ctaaaaaata atagatggaa aaattacagt
      301 tctttctctc acagagagct tgtggttgct gaaaacaagc agagaaagct gtaaagttgg
      361 gttcatctga aacgagagaa gaaaacatca ttctcaaggg agtgcccttt atcgcacagc
      421 ctgttacagg agatatctga gacaggaatg agagatagga gaaatcctat aattgaggtg
      481 gcaccgcgaa tttcgtcctc acgcaagtta ttttgcgtgg ggatttttca tacagtcacc
      541 acggactaga agtagaactg aaagggaaat tacaatggaa cgaatcattc atggagatgt
      601 cttatcacca atcttggctt atatgcgctt aaaggggcaa cataaggtta ttttagagag
      661 tattccgaga gacaaggaaa cagctcgttt ttatatccta gcctataatc cagtttttga
      721 gattaagttt gaaaatgggg ttctctatca aaatggtcaa gtgattgatc gggatccctt
      781 ggatttcctt tatgaagtga ttcataagag tcagcaccac tcagagctac cttttggtgg
      841 tggagccatt ggttttgtgg gttacgatat gatttcgctt tatgaagaaa ttggtcaaat
      901 ccctgaggat acaattggga cgccagacat gcatttcttt gtctatgaga gctatatggt
      961 ctttgaccac aagaaggaga agatacatgt catagaggat gcactttata gtgagcgcag
     1021 tcaagaggcc ttggaaaaat ccttgaacca agtgcttgag gaattacgca ttcctgctcc
     1081 aaatgaattt gaagatttgg atctatctcc gttagacttc aaaccgcata tcgctccaca
     1141 taagtttgag ggaatggtag aaacagctcg tgacttgatt cgtaacggag atatgttcca
     1201 atgtgtactc agccagcgtt tttcagcaga agttactgga aatccatttg acttctacag
     1261 aaatctccgc gtgactaatc cttctaatta cctttatttc tatgattttg gggattatca
     1321 aatcatcgga gctagtccag aaagtttagt ttctgtcaaa aatggcatcg tgacaaccaa
     1381 tccgattgca gggacgcgac caagaggggc tacggatgaa gaagacaaga ccttggcgac
     1441 tgacctcctc tctgatgaga aggaaacagc agaacatcga atgttggtag acttggggcg
     1501 taacgatatt ggccgcatct ctgaaacggc cagtgtccaa gttactaagt atatgaaggt
     1561 ggagctcttc cgctacgtca tgcatttgag cagcgtggtc aaggggcgtt tgcttccaga
     1621 actcacggcc atggatgcct tgaaagctac acttcctgct ggaaccgttt ctggagcacc
     1681 aaagattcgg gcgatgagac gcatctatga actggaaacg gaaaaacggg gcgtatatgc
     1741 aggagcaatc ggctacttgt ctgcgacggg tgatatggat ttggccattg ccatccgaac
     1801 catgattctc aaaaatcaaa gagcctatgt gcaggctggg gcagggattg tctacgactc
     1861 tatcgcccaa aacgaatacc aagaaaccat taacaaagca aaatctatga ctagaattgg
     1921 agaactaaga ccatgatttt attgattgac aactatgatt cttttaccta taacttggcc
     1981 caatacattg ggaattttgc agaagttcag gttctgagaa atgatgattc caagctgtat
     2041 gaagaagctg aaaaagcaga tggtctggtt ttttctcctg gtcctggttg gccagttgat
     2101 gctggaaaga tggaagacat gattcgtgat tttgcaggca agaagccgat tcttgggatt
     2161 tgtttgggcc accaagccat tgcagaagtc tttggtggta agctaggttt ggctccaaaa
     2221 gtcatgcatg ggaaacagag caatatcaac tttgaagcgc catctgtttt gtatcaaggt
     2281 attgaggaag gacgtgcggt catgcgttat cacagtattt tgattgagga aatgccagaa
     2341 gactttgaag tgacagctcg ttcgactgat gaccaagcca tcatggggat tcaacataaa
     2401 aacctaccga tttatggctt ccagtaccat ccagagagca ttggaacgcc agacggcttg
     2461 tcttctattc ggaattttat cgagaaggtt gtaaagtgag gaaactagga tgaaagagat
     2521 tattgaaaaa ctagcaaaat ttgaaaattt atcaggtgtg gaaatgacgg atgtcattga
     2581 gcgtatcgta actgggcgtg taactgaagc gcagattgct tctctcctct tagctcttaa
     2641 gatgaagggg gaaacacctg aagaacgcac agccattgcc caagtcatga gaggacatgc
     2701 ccagcatatt ccaactgaga ttcatgatgc catggacaac tgtggtacag gtggggacaa
     2761 gtctttcagt tttaatattt ccacaactgc agcctttgtc ttggctggtg gcggtattca
     2821 catggccaag catggtaacc gctcgatttc ttctaaatct ggttccgcag atgtcctcga
     2881 agccttggga atcaatcttg acctcaaacc agctgaacta ggtaaggtct ttgataaaac
     2941 tggaatcgtc tttctcttcg ctaaaaatat gcacccagct atgaaataca tcatgccagc
     3001 tcgtttggaa ttgggaattc caacgattat gaacttgact ggtccactga ttcatccaat
     3061 ggctttagaa acacagcttc ttggaattag tcgtccagaa ctcctagaaa gtacagctca
     3121 ggttttgaaa aatatgggtc gcaaacgtgc catcgtggtt gctggaccag aagggttgga
     3181 tgaagctggc ttgaacggaa caaccaagat tgcacttctt gaaaatggcg aaatcagctt
     3241 gtcaagcttt actccagagg atttgggaat ggaaggctat gctatggaag atattcgtgg
     3301 tgggaatgct caggaaaatg cagaaatttt gcttagcgtt ctgaaaaacg aagcaagtcc
     3361 attcttggaa acgacagtct tgaatgctgg tcttggtttc tatgctaatg gtaagattga
     3421 tagcatcaag gaaggagttg ccttggcccg tcaagtgatt gctagaggca aggcccttga
     3481 aaaactcaga ctgttacagg agtaccaaaa atgagtcagg aatttttagc acgaatctta
     3541 gagcagaagg tgcgtgaggt ggagcagatg aagctggagc aaatccagcc tctgcgccag
     3601 acctatcgct tggcagagtt tttgaagaat catcaggacc gcttgcaggt aatcgctgag
     3661 gtcaagaaag ctagccctag tttgggagat atcaatctcg atgtggatat tgtgcaacag
     3721 gcccagactt atgaagaaaa cggagcagtg atgatttcgg tgttgacaga tgaggttttc
     3781 tttaaagggc atttggatta tctacgggaa atttccagtc aggtagagat tccgacgctc
     3841 aacaaagact ttatcatcga tgaaaagcaa atcatccgcg ctcgcaatgc aggtgcgaca
     3901 gttatcttgc tcattgtggc agccttgtcc gaagaacgcc tcaaggaact gtatgactac
     3961 gcgacagagc ttggtctgga agtcttagtg gagactcaca atctagctga actagaggta
     4021 gcccacagac ttggtgctga gattatcggg gtcaacaacc gcaacttgac tacctttgag
     4081 gtcgacttgc agaccagtgt agatttagcc ccttactttg aggaaggtcg ctattacatt
     4141 tctgaatctg ccattttcac agggcaggat gcggaacgac tagccccata ctttaacgga
     4201 attttggtag ggacagctct tatgcaggca gaaaatgtgg cccagagaat caaggagttg
     4261 cagattgaca aaggttaaaa tttgtggact atcgaccaaa gaagcggtgg aaacagccgt
     4321 ttcagcagga gccgactata tcggttttgt ctttgcacct agtaaaagac aggtaacttt
     4381 agaagaagta gctgagttgg caaagcttat tcctgcagat gtcaaaaagg ttggtgtatt
     4441 tgtttcacca agtcgggcag aactgctgga agcgattgac aaagttggct tggacttggt
     4501 tcaagttcac ggtcaggtgg cagatgattt atttgagaat ttgccttgtg ctagcattca
     4561 ggctgtgcag gtagatggag agggtcatgt gcccaattct caggcagact atctactctt
     4621 tgatgcccct gtggctggga gtggccagcc ctttgattgg ggtcaactgg atacgactgg
     4681 actagcacag cccttcttta tcgcaggtgg ccttaatgaa gataatgtag taaaagcaat
     4741 tcaacacttt actccctatg cagtagatgt atcaagcgga gtggagacag atggacaaaa
     4801 agatcatgaa aagattagaa gatttataga gagggtaaag aatggcatat caagaaccaa
     4861 ataaagatgg attttacgga aaatttggcg gacgttttgt cccagaaaca ttgatgacag
     4921 cagttttgga gttggagaag gcctaccgtg aaagtcaggc agacccaagt ttccaagagg
     4981 aattaaacca gcttttgcgc cagtatgtgg gacgtgaaac tcccctttac tacgcaaaaa
     5041 acttgaccca gcatatcggc ggagccaaga tttatctcaa acgggaagac cttaaccata
     5101 caggagccca caagattaac aatgccttag gacaagtttg gcttgccaaa cgcatgggta
     5161 aaaagaaaat tatcgcagaa acgggtgctg gtcagcacgg tgtggcaact gcaactgctg
     5221 cggccctctt taacatggaa tgtaccatct acatgggtga ggaagatgtc aaacgccaag
     5281 ccctcaatgt cttccgtatg gagcttttgg gagctaaggt cgaggccgtg acagatggtt
     5341 cgcgcgtgct caaggatgcg gtcaatgcag cccttcgttc atgggtggct aatatcgacg
     5401 atacccacta tatccttggt tctgccttgg ggcctcatcc atttccagaa atcgttcgtg
     5461 acttccaaag tgtcatcggt cgagaggcta aacaacagta ccgtgacttg acaggtcgag
     5521 atctgccaga tgccctagta gcctgtgttg gtggtgggtc gaatgctatc gggctcttcc
     5581 atccctttgt agaagatgag tcagtagcta tgtatggaac tgaagcagca gggcttggtg
     5641 tggatacaga gcaccacgct gctaccttga ccaagggtag tccgggtgtt cttcacggtt
     5701 ctctcatgga tgtgctccaa gatgcccatg gtcaaattct tgaagccttc tctatctcag
     5761 caggtttgga ctatcctggt atcggtccag agcattctca ctaccacgat atcaaacgtg
     5821 ccagctatgt ccctgtgaca gacgaagaag ctttggaagg atttcaactc ttgtctcgtg
     5881 tggaagggat tatcccagcc ttggaatcta gccatgctat cgcttttgcg gtgaaattgg
     5941 ccaaagaact tggaccagaa aagtctatga ttgtctgcct atcaggtcgt ggggacaagg
     6001 atgtggttca agtcaaagac cgcttggaag cagatgcagc aaagaaggga gaagctcatg
     6061 cctaagacac taacagaaaa attgaatgct ataaaagtaa ctggaaaagg aattttcgtt
     6121 ccttatatca tggctggtga ccacgaaaaa ggtttggacg gtcttgctga aacaattcac
     6181 tttttagaag atttgggtgt ctctgctatt gaagtgggga ttcccttttc agaccctgtt
     6241 gcagatggac ctgttatcga agaagctggc ttgcgcagtt tagcccacgg gacctctacc
     6301 caggctttag ttgaaacctt gaaaaccatt gaaacagaga ttccactggt catcatgacc
     6361 tacttcaacc ccctctttca gtacggtgtg gagaactttg tcaaagatct ggctgataca
     6421 gctgtgaagg gcttgattat cccagacctg cctcatgaac atgccaactt tgtagaacca
     6481 tttttggcag atacagatat cgccttgatt cctttagtaa gcttgaccac aggaattgag
     6541 cgccagaaag agttgattga aggggcggag ggattcgtct atgccgttgc catcaatggg
     6601 gtgacaggga aatcaggcaa ttaccgtgca gatttggaca agcacttggc gcaacttcat
     6661 caagtggccg acatcccagt cttgacaggt tttggtgtat ctagtcaagc cgatctggaa
     6721 cgcttcaatg cggtgtcaga tggtgttatc gtcggttcaa aaatagtaaa agctctccac
     6781 caaggagagc cgattcagga ttttatcaga caagcagtag cttaccaaaa ataatcaaac
     6841 aagtagcaag taagaggaaa ggcacgaaag gaagtctttc ctttttcttt tgcaggagaa
     6901 aggtcagttt tatagtgaat tgaatctgga atattacact acggttccta aaatattttc
     6961 agaaagcgat ttgaatttcc caataaattt gttcagtttt taattgattt tactatagaa
     7021 gatataattt tctaaattac tctgacacag aactttaaga aaaagctaat tattgtagta
     7081 atttctttaa gaaaatgata taattcatta aaaagtattt ggaggaggaa gaattatgtt
     7141 gaatcaggat ctctttgatt cgcttgaggc acaaaaaatt gtagatactt tgatgaaagg
     7201 gcaaaaagat tatgtagatg aacgtctaga aaaaagagag acaatgatag tgtcgaatgg
     7261 ttatgcatgg acgcgaccta atcatattga tactgcattt gcatcagcag atttgtttga
     7321 gtataaatta caattagcag gacagacttg gggatattta gaatttgaaa caaatacaga
     7381 aaaatatggg aaagtattgt taattataaa gggtaagaag cgacttacga accaatttcc
     7441 tttggtacaa aaaaataaga gtggctactt atttgaatat gctcagatga atacacttta
     7501 tcttaatcaa cattcttcct acaaaaatga tgaaaatagt cattcctttc caattcagat
     7561 ggagttagtt tctgatgaaa tgattcaaga aattgaacaa gctactaaaa attcgaatat
     7621 cgaaaaattt atgattttaa cttatgaggc ggactcagaa aacaatatta tatctgtaga
     7681 tgttgttatg cctgatgcac gaactggtca gttacacttg attcaagatt tgtctgagta
     7741 tattcaatca agttcgtacc atttcgaaga agctaaatat caagatattc ctaatttttc
     7801 agaattatcg gaaacagaag attttgaaat tattccaaga atagaaaaac aagaaggtca
     7861 aaagtagtaa ggagtatgta aatgtttaat ggtcgggtat tgaaagaatt acggctgtta
     7921 aatggtttaa gtagagcaga attagctcag agaattaatt taacggaaca agccatttgg
     7981 cagtttgagt ccaacgaaac gaaacctaaa ttatcaacca aaatgcattt ggccaaccaa
     8041 tttcatgttg atttaactta ttttgaacag gaagaagaga gcattcgatt tgattcttct
     8101 gtaattgcct ttagaaatgc agacctagca acacggaaaa caatagatat tcaaactatg
     8161 tatttacata aggtagatag tttgattgat tattttgaaa gttttgtaat tatacctaat
     8221 attataattc atgacctaag taatgtagtg agtgaatctt atcataaggg agaatccatt
     8281 gaggaattgg ctctttatgc cagggaaaaa ttaggtattt caaaagataa tcatgatttg
     8341 ctttataaat tagaacgttc aggcatctat atcgtggaac gattaattaa tggccaagct
     8401 gatgcttata gcgcatggtc aaaattggga agaccttata ttgtgttggg aacgaataaa
     8461 tcatctgtac gtcgaaattt tgacttagct catgagctag gacatattct tttacataaa
     8521 tataaagata tgaatgaaga tggcgatcgt ttggagcaag aagcaaatta ttttgcatca
     8581 tgttttttat tgccaaaaga agagttttta gtcaaatttg aagagagggt tggcaagcgt
     8641 gtcagcaatc ctgatagtta tattttattg aagtcggatt tgaatgtttc gatacaggct
     8701 ttagagtatc gagcttttaa gttaggatta ttgactccaa agcaacattc ttacttttat
     8761 cgtcaaattg cgcaaaaagg ttacaaaatg attgaacctt tggatgatca aatttttgtt
     8821 aaaaaaccaa gcaaagtaaa gagtattctg gacgtcgttt tgagtaatca tctagtcagt
     8881 ctagcgacta taatgtctaa acaaagtatt cgtttacagt ttataagcga aatattttca
     8941 gtcgaaatga aattttttga tcagtatcaa gaagatagaa gaacagatcg atttgataac
     9001 atcatccctt tgtacaaaag aaataattta taatttaact gtgaaataat ttcatgaagg
     9061 acatatggca gaaattggat tttatcagac aagcagtagc ttaccaaaaa taatcaaaca
     9121 agtagcaagt aagaggaaag gcacgaaagg aagtctttcc tttttctttt gcaggataaa
     9181 ggccaggata cccgtcgcag aagcaaactg aatcaagatc agtaactccg ttacgctaaa
     9241 gacgagagca caagaagcta aaaagaggaa atctcctgcg cccatgcgga tatcgataaa
     9301 atgagccaaa attccaagag caaggaagga gaccatgacc agattccagc cagaggaagc
     9361 tattaggatt aggtggaaag tcatccagac cagtaaggga tattcctgat ggtgaaagtc
     9421 gtagataccc aaggtcaaac cagcggtgat taggacgact tgccccaagg aaagcaattc
     9481 ccaagagtaa agcagaaaga ggagtcctaa gactaattca aagagggcat accagacagg
     9541 atagcgaact ttgcagtagc gacagcgaaa gcgattgaag acctgtgaga gaatcggaat
     9601 caaatctaag ggacgcaagc gagtctgaca ggaatcgcag tgactggctg aactgataat
     9661 ggatttctct ggaaaacggt caatgaccaa accaagaaag gaagcgagaa tgctcccgac
     9721 gagaaaaaaa taaaaatcaa tcatacttat ctattcgtaa aaaatgggag aaatagtata
     9781 atggagaaac atagataaga tggtgaggaa aagatgacaa ttcgttttga agaaaaggtg
     9841 agtatagaaa acgctcaatt tgtatgccag tggtccaact ccctaggcaa atcctttcaa
     9901 gaacaatgga tgggaccaag gattcctttt ctactgaccc ttcaagcctt ggaaggagtc
     9961 ttttcaatct ttgatgaaca agagtttgtg ggacttatcc agaaaatcag gctagaagac
    10021 agcaatcttc atatcgggag attttttatc aacccccaga aacaggagca agacttaggt
    10081 agccaggctt taaggaaatt tgttagtttg gcctttgaaa atgaagatat agatagtatt
    10141 tctctaaatg tcttcgaggc aaatcaaaga gctcagaatc tttaccaaaa agaaggattt
    10201 gaaatcgttc aaatg
//
LOCUS       DAVKGU010000072        10124 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKGU010000072 DAVKGU010000000
VERSION     DAVKGU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.072"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            16..879
                     /locus_tag="VW077_001613"
                     /pseudo
     CDS             16..879
                     /locus_tag="VW077_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1262..1882
                     /locus_tag="VW077_001614"
     CDS             1262..1882
                     /locus_tag="VW077_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455606.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTCKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1968..2411
                     /gene="tsaE"
                     /locus_tag="VW077_001615"
     CDS             1968..2411
                     /gene="tsaE"
                     /locus_tag="VW077_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9455607.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2401..2919
                     /locus_tag="VW077_001616"
     CDS             2401..2919
                     /locus_tag="VW077_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455608.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVCHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            2927..3943
                     /locus_tag="VW077_001617"
     CDS             2927..3943
                     /locus_tag="VW077_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9455609.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4028..5284
                     /locus_tag="VW077_001618"
     CDS             4028..5284
                     /locus_tag="VW077_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9455610.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGASSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5339..6505
                     /gene="recA"
                     /locus_tag="VW077_001619"
     CDS             5339..6505
                     /gene="recA"
                     /locus_tag="VW077_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9455611.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            6812..8182
                     /locus_tag="VW077_001620"
     CDS             6812..8182
                     /locus_tag="VW077_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9455612.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKQPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8558..9514
                     /gene="lytA"
                     /locus_tag="VW077_001621"
     CDS             8558..9514
                     /gene="lytA"
                     /locus_tag="VW077_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9455613.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
BASE COUNT         3086 a         1729 c         2247 g         3062 t
ORIGIN      
        1 ggtttattat ctttaatgag tacactcgca gagaaattca gattgaaaag aaaagagttg
       61 agactctccc aacaaactct tgcagaagga atttgtgaac aaagccagat tagtaaaatt
      121 gagagagggc atttcattcc ctccgcagac cttttgttca aactctctca acgacttgaa
      181 gtaccattag attatttttt taatgaacaa attgaaatta aatctaacct ctctaatttc
      241 aagcaattgt ctgctcgact attagacgat agaaattatg acgatttgga atatatttat
      301 agaatagaga ttgaacgaag tacttttcta acactagaag accgaactta ccttgaatgg
      361 attaaagcta ttattgactt ctatcaatat gacagtaagt gtgaggcaat ttcttcattg
      421 gaaaatatat tattaaaagt ctcttcaaat actctgattt atttaaaggc gttgaatact
      481 ctatctaatt tctattcctt agtgggtcgt gaacaagaat atgaggcaaa ctactctcat
      541 ttaatagagt tgtatcagac aaaaaatttg gaccatcaag agtttttatt tggctacatc
      601 agagttcgtt acaactacgc tcactaccta gtatcaaagg aaaaatataa tgaagccatc
      661 taagaagctc ttgaaacgat tgaactctgt aaacaaagat agacaagcta ccaattggct
      721 cctctactta ttcttgtagg aaatgctgga gccaaatttc tagacagaga acaagtcaaa
      781 aattattata tagaagcaag agagctatgc aagatttata acaatccttt aatgctgatg
      841 aagatagaaa attatttgaa ggaactagat gctgtttagt ttatcttgat attggagtgt
      901 tacttaaaca ttaaatagcc tgtaaggctg atagttagat taatactatc agccttataa
      961 ttatgtgact ctgagtccgt ctcgctccgt gaggtgcgag acaaaacaac cacccgtttg
     1021 acggattttc atgatttcaa atttatattc ttcgaaaatc tcttcaaacc acgtcagctc
     1081 tatctgcaat ctcaaaacag tgttttgagc aacctgtggc tagctaccta gtttgatctt
     1141 tgattttcat tgagtattag tctctagtgt ttgtttagcg tcttaaagag acatttgaga
     1201 agattagaaa cagttctctc ttttctccta gtataatgat tggtaggaag ggagagagaa
     1261 gatgaaatca atgagaatct tatttttgtt agctttaatt caaatcagtt tgagtagctg
     1321 tttcctatgg aaggaatgca tcttgtcctt taaacaaagt acagcttttt tcatcggaag
     1381 catggttttc gtttcaggaa tctgtgctgg agtaaattat ctttatacct gtaagcaaga
     1441 agtccatagt gtcctagcca gtaagaagtc ggtgaagctt ttttacagta tgttactctt
     1501 aattaatttg ttaggagctg ttcttgtttt gtcagataac ttgttcatca aaaatacgct
     1561 gcagcaagaa ttagttgact ttttattgcc atccttcttt ttcctatttg ggctagattt
     1621 gctgattttt ttacccttga aaaaatacgt gcgcgatttt cttgctatgc tagacagaaa
     1681 aaagacagtg ttggtgacta ttttagcaac acttcttttc ttaagaaatc caatgaccat
     1741 tgtctcactt ctgatttata ttggattggg cttgtttttt gcagcctatc ttgtcccaaa
     1801 ttcggttaag aaggaagttt ccttttatgg tcatattttc cgagatcttg tattggtcat
     1861 tgttacgctc attttctttt agagaaagcc tgttttttat ggatgttatg atgataagtt
     1921 tagtaggaaa atagacacaa aagaaaagtt tttggtataa taagagtatg tacacaaaaa
     1981 atgaagaaga gttgcaagcc ttaggggagc gtttgggcca tctattagca aagaatgatg
     2041 ttttaatctt aactggagaa ctgggtgcag gtaaaacgac ctttactaaa ggacttgcaa
     2101 aaggattaca gatttctcaa atgattaaaa gtcctaccta tactatcgtg agagagtatg
     2161 aaggtcgact tccactttat cacctagatg tttatcgtat tgaaggagat gctgattcta
     2221 tcgacttgga tgagtttatc tttggtggcg gcgtgactgt tattgagtgg ggaaatctct
     2281 taggagatgc cttgccagat gcttatttag aattggaaat tctaaaagaa gcagatggac
     2341 gccgtttaaa ttttcaggca aagggtttgc gtgctgagaa attgttagag gagcttcaat
     2401 atggagtatg aattgctcat tagggaagca gagcccaaag atgcagctga attagtggcc
     2461 tttttaaatc gtgtgagttt ggagacagac tttaccagcc tagacggaga tggtattctc
     2521 ttgactagtg aggagatgga aatattcctc aacaagcaag ctagttcgga caatcagata
     2581 accttacttg cattcttaaa tggcaaaatt gctggtattg taaatattac agctgaccag
     2641 cgtaagagag tctgtcatat tggagatctc ttcattgtga ttggaaaaag atattggaat
     2701 aatggcttgg gaagtttgtt gctagaagaa gcgatagagt gggcacaagc aagtggcatt
     2761 ctgcgtcgtc tccaactgac tgtccaaact cgtaatcaag cagcagtcca tctttatcaa
     2821 aagcatggct ttgtcattga aggtagccaa gagcgtgggg catatataga agaagggaaa
     2881 tttatcgatg tttacttgat gggtaaactg atagattagt agaaatatgg ttaaaaaaat
     2941 tattggaatg gtgctagctt tactttctgt aactgtagta ggagtaggtg tttttgctta
     3001 tactatttat caacaaggga cagaaacctt agctaaaacc tataaaaaaa tcggcgaaga
     3061 aaccaaggtt attgaagcga ctgaacctct aaccattctg ttaatgggag tggacaccgg
     3121 aaatgctgaa cgaactgaaa cttgggtcgg tagaagtgat agcatgatct tgatgacagt
     3181 gaatcctaaa acgaaaaaaa caacaatgat gagtttagag cgggatattc tgacgcgcat
     3241 tgaatcaggg aatggtcagg ctcatgaagc gaaactgaac tcagcatatg cagatggtgg
     3301 agcagagctt gctatagaaa ccattcaaaa aatgatgaat atccatattg atcgctatgt
     3361 gatggtcaat atgagagggt tgcaaaaact agtggatgca gtaggaggta ttacagtcaa
     3421 taatatccta ggtttcccaa tttctatcag tgaccaagaa gaatttaata ctatttctat
     3481 cggtgttggg gagcaacata ttgggggaga agaagcccta gtctatgcac gaatgcgtta
     3541 ccaagatcct gagggggatt atggtcgtca aaaacgtcaa cgtgaagtta ttcaaaaagt
     3601 catggaaaaa gctctcagtt taaatagcgt tggtcattat caagagattc taaaagcttt
     3661 gagtgacaat atgcagacca atattgattt gtctgcaaaa agtatcccta acttgctagg
     3721 ctataaagat tcatttaaaa ccattgaaac tcagcagttg cagggtgaag gagagatact
     3781 tcaaggtgtt tcttaccaga ttgtttcgag agcacatatg ttggaaatgc aaaatatact
     3841 ccgacgttct ttgggacaag aagaagttac tcagcttgaa accaatgcgg ttttatttga
     3901 agatttattt ggcagagcac ctgttggtga tgaagataat taacttttga tacaaataaa
     3961 aaaatcaatc gtaggaaaat tcctgcgatt ttttcaaaaa aatacgaata gataggtagg
     4021 aggaaacatg aaagcagaaa tcattgctgt tggaacagag attttgacag gacagattgt
     4081 caacaccaat gcccagtttt tgtcggaaaa actagctgag attggggtag atgtatattt
     4141 tcagacggct gtaggagaca atgaagttcg tctcttgtct ttgcttgaga ttgccagtca
     4201 acgtagcagt ctggtgattt tgacaggcgg tttggggcca actgaggacg acctaaccaa
     4261 acaaacccta gctaaatttt tagggaaagc attagtcttt gatcctcagg ctcaggagaa
     4321 gttggatatc ttttttgccc tgcgaccaga ctatgcccga acaccgaata acgaaagaca
     4381 agctcaaatt gtagaaggag cgattccact gccaaacgaa acaggactgg ctgttggagg
     4441 aaaattagaa gtagacggag tgacctatgt cgtccttcca ggtccgccaa gtgaattgaa
     4501 acccatggtc ttaaaccaac ttctacccaa gttgatgaca gggagcaagc tgtattcccg
     4561 agttcttcgt ttctttggga ttggcgagag ccagttggtt acgattttgg ctgatttaat
     4621 tgataatcag atcgatccta ccttggcccc ttatgccaag acaggagaag tcactctacg
     4681 tctgtcaaca aaggctagca gtcaagaaga ggcgaatcaa gcgctggata tcttggaaaa
     4741 tcaaatcttg gactgccaga ctttcgaagg aatttcttta cgagactttt gctatggtta
     4801 tggggaagaa actagtttag ccagcattgt ggtagaagaa ctgaaaaggc aagggaaaac
     4861 catcgcggct gcagagagtt taacggcagg tcttttccaa gctaccgtgg cgaatttttc
     4921 tggagcttca agtatattta agggtggttt tgtgacctat agcttggagg aaaaatcaag
     4981 gatgttggat attcctgcca agaatttgga agaacatggt gtggtgtctg aatttacagc
     5041 tcagaagatg gctgagcagg cacgaagcaa gacccagtct gattttggaa ttagtttgac
     5101 cggagtggca ggaccagata gcctagaagg acacccagtt gggacagtct tcataggctt
     5161 ggcgcaagag caaggaactg aggttatcaa ggttaatatt ggaggcagaa gccgagcaga
     5221 tgtacgtcat attgcggtta tgcatgcctt taacctagtt cgcaaggctt tattaagtga
     5281 ctaacttttg atataatagt agataggtct gaggatcatt agaatgtagg agaatagaat
     5341 ggcgaaaaaa ccaaaaaaat tagaagaaat ttcaaaaaaa tttggggcag aacgtgaaaa
     5401 ggccttgaat gatgctctta aattgattga gaaagacttt ggtaaaggat caatcatgcg
     5461 tttgggtgaa cgtgcggagc aaaaggtgca agtgatgagc tcaggttctt tagctcttga
     5521 cattgccctt ggctcaggtg gttatcctaa gggacgtatc atcgaaatct atggcccaga
     5581 gtcatctggt aagacaacgg ttgcccttca tgcagttgca caagcgcaaa aagaaggtgg
     5641 gattgctgcc tttatcgatg cggaacatgc ccttgatcca gcttatgctg cggcccttgg
     5701 tgtcaatatt gacgaattac tcttgtctca accagactca ggagagcaag gtcttgagat
     5761 tgcgggaaaa ttgattgact caggtgcagt tgatcttgtc gtagtcgact cagttgctgc
     5821 ccttgttcct cgtgcggaaa ttgatggaga tatcggagat agccatgttg gtttgcaggc
     5881 tcgtatgatg agccaggcca tgcgtaaact tggcgcctct atcaataaaa ccaaaacaat
     5941 tgccattttt atcaaccaat tgcgtgaaaa agttggagtg atgtttggaa atccagaaac
     6001 aacaccgggc ggacgtgctt tgaaattcta tgcttcagtc cgcttggatg ttcgtggtaa
     6061 tacacaaatt aagggaactg gtgaccaaaa agaaaccaat gtcggtaaag aaactaagat
     6121 taaggttgta aaaaataagg tagctccacc gtttaaggaa gccgtagttg aaattatgta
     6181 cggagaagga atttctaaga ctggtgagct tttgaagatt gcaagcgatt tggatattat
     6241 caaaaaagca ggggcttggt attcttacaa agatgaaaaa attgggcaag gttctgagaa
     6301 tgctaagaaa tacttggcag agcacccaga aatctttgat gaaattgata agcaagtccg
     6361 ttctaaattt ggcttgattg atggagaaga agtttcagaa caagatactg aaaacaaaaa
     6421 agatgagcca aagaaagaag aagcagtgaa tgaagaagtt acgcttgact taggcgatga
     6481 acttgaaatc gaaattgaag aataagctgt taaagcagtg gagaaatccg ctactttttc
     6541 gatttttgat tcaagttttt agattatata tagtagcttg aaataagata tgaacaactc
     6601 tattaggaaa gtcaaattaa tttctagaaa tgttttagca gctacagcgt actattccaa
     6661 actcaaccaa ctataataga tcgaaactag aatagtacat atctacttct aaaacattgt
     6721 taaaaatcga tttgactttc cttatttcat tccgctatat ataagtttgc tgtttcttgt
     6781 cgctcctctg gaaagctgat ataatagctt tatgaataaa aaacgaacag tggacctgat
     6841 acatggtccg attcttccct cgctcttaag cttcaccttt ccaattttgc tatcaaatat
     6901 ttttcaacag ctctataaca ctgctgatgt cttgattgtt ggacgatttc ttggtcaaga
     6961 atccttggct gcagtaggag cgacgacagc gatttttgac ctgattgtag gttttacact
     7021 tggtgttggc aatggcatgg ggattgtcat tgctcgttat tatggggctc ggaatttcac
     7081 taaaatcaag gaagcagtag cagctacttg gattttaggt gctcttttga gcattctagt
     7141 tatgttgctg ggctttcttg gcttgtatcc tctcttgcaa tacttagata ctcctgcaga
     7201 aattcttcct caatcttatc aatatatttc tatgattgtg acctgtgtag gtgtcagctt
     7261 tgcttataat ctttttgcag gcttgttgcg gtctattggt gacagtctag cagccctggg
     7321 atttctgatt ttctctgcct tggttaatgt ggttctggat ctctatttta ttacgcaatt
     7381 gcatctggga gttcaatccg caggacttgc taccattatt tcgcaaggtt tatcagcggt
     7441 tctctgcttt tattatattc gtaaaagtgt gccagaactc ttgccacagt ttaaacattt
     7501 caaatgggac aaaagcttgt acgcagatct cttggagcaa ggtttggcta tgggcttgat
     7561 gagttcaatt gtatctatcg gcagtgtgat tttacagtct tctgttaata catttggtgc
     7621 agtgattatt agtgcccaga cggcagctcg acgcattatg acctttgccc ttcttcctat
     7681 gaccgctatt tctgcatcaa tgacgacctt tgcttctcag aatctaggag ctaagcaacc
     7741 tgaccgcatt gttcaaggtc ttcgaatcgg cagtcgttta agtatatcct gggcagtttt
     7801 tgtttgtatt ttcctctttt ttgccagtcc agccttggtt tccttcttgg ctagttcgac
     7861 agatggttac ttgatagaaa atggaagtct ctatctgcaa atcagttcag ccttttatcc
     7921 cattttgagc ctcttgttga tttatcgcaa ttgcttgcag ggcttggggc agaagatcct
     7981 tcctctagtt tctagcttta ttgaactaat cggaaaaatc gtttttgtgg ttttgattat
     8041 tccttgggca ggatataagg gtgttatcct ttgtgaacct cttatctggg ttgccatgac
     8101 agttcaactg tacttctcac tattccgtca tcccttgata aaagaaggaa aggcaatttt
     8161 ggcaaccaaa gtgcaatcct agttggattt actgaataaa atccatttcc tctagtgaaa
     8221 atcgaaaaaa cttgtgttct cttctttagt ttggtgttga aaatagttta acagactttt
     8281 gacttctttt atatgatata ataaagtata gtatttatga aaaggacata tagagactgt
     8341 aaaaatatac ttttgaaaag ctttttagtt tggggtgtta ttgtagatag aatgcagacc
     8401 ttgtcagtcc tatttacagt gtcaaaatag tgcgttttga agttctatct acaagcctaa
     8461 tcgtgactaa aattgtcttc tttgtaaggt agaaataaag gagtttctgg ttctggattg
     8521 taaaaaatga gttgttttaa ttgataagga gtagaatatg gaaattaatg tgagtaaatt
     8581 aagaacagat ttgcctcaag tcggcgtgca accatatagg caagtacacg cacactcaac
     8641 tgggaatccg cattcaaccg tacagaatga agcggattat cactggcgga aagacccaga
     8701 attaggtttt ttctcgcaca ttgttgggaa cggttgcatc atgcaggtag gacctgttga
     8761 taatggtgcc tgggacgttg ggggcggttg gaatgctgag acctatgcag cggttgaact
     8821 gattgaaagc cattcaacta aagaagagtt catgacggac taccgccttt atatcgaact
     8881 cttacgcaat ctagcagatg aagcaggttt gccgaaaacg cttgatacag ggagtttagc
     8941 tggaattaaa acgcacgagt attgcacgaa taaccaacca aacaaccact cagaccatgt
     9001 ggatccatac ccttacttgg caaaatgggg cattagccgt gagcagttta agcatgatat
     9061 tgagaacggc ttgacgattg aaacaggctg gcagaagaat gacactggct actggtacgt
     9121 acactcagac ggctcttatc caaaagacaa gtttgagaaa atcaatggca cttggtacta
     9181 ctttgacagt tcaggctata tgcttgcaga ccgctggagg aagcacacag acggcaactg
     9241 gtactggttc gacaactcag gcgaaatggc tacaggctgg aagaaaatcg ctgataagtg
     9301 gtactatttc aacgaagaag gtgccatgaa gacaggctgg gtcaagtaca aggacacttg
     9361 gtactactta gacgctaaag aaggcgccat ggtatcaaat gcctttatcc agtcagcgga
     9421 cggaacaggc tggtactacc tcaaaccaga cggaacactg gcagacaagc cagaattcac
     9481 agtagagcca gatggcttga ttacagtaaa ataataatgg aatgtctttc aaatcagaac
     9541 agcgcatatt attaggtctt gaaaaagctt aatagtatgc gtttttatcc tctagtcact
     9601 tgatttgttt caggtggttt tttagtatag tagaatgaaa cgagaacagg acaaattgat
     9661 caggacagtc aaatcgattt ctaacaatgt tttagaagca gaagtgtact attctagttt
     9721 caatctacta tagttaaatc tgcggtcaag tctactggtg aatctatgat tgtaatactc
     9781 ttccaaaatc tcatcaacca cgtcagtctt gccttgcagt ctgtatctta ctgaccaagc
     9841 tagtgatgga tttagaatag gtgatttgga gcgtcctatt ggctaggaaa tgctgctcat
     9901 agtcctttgc tgaggctagg gtgtttcaac attcaacact caactggttg atctagttga
     9961 taggaaggga attactataa aatactcagg cttccatcat attttttgaa acgattgtgt
    10021 aatcaaaatg taccaatatt gtagtattgg tacagaagat gttgtgaatg gataaatata
    10081 tcataactgc tgtctcaaaa agatttcata tgtctgtgca tata
//
LOCUS       DAVKGU010000073         9853 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKGU010000073 DAVKGU010000000
VERSION     DAVKGU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.073"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            11..490
                     /gene="rlmH"
                     /locus_tag="VW077_001622"
     CDS             11..490
                     /gene="rlmH"
                     /locus_tag="VW077_001622"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9455614.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            534..607
                     /locus_tag="VW077_001623"
     tRNA            534..607
                     /locus_tag="VW077_001623"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:568..570,aa:Arg,seq:tct)
     gene            772..897
                     /gene="comC"
                     /locus_tag="VW077_001624"
     CDS             772..897
                     /gene="comC"
                     /locus_tag="VW077_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9455615.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            918..2243
                     /locus_tag="VW077_001625"
     CDS             918..2243
                     /locus_tag="VW077_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9455616.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            2240..2992
                     /locus_tag="VW077_001626"
     CDS             2240..2992
                     /locus_tag="VW077_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9455617.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            3044..3115
                     /locus_tag="VW077_001627"
     tRNA            3044..3115
                     /locus_tag="VW077_001627"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3077..3079,aa:Glu,seq:ttc)
     gene            3121..3194
                     /locus_tag="VW077_001628"
     tRNA            3121..3194
                     /locus_tag="VW077_001628"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3155..3157,aa:Asn,seq:gtt)
     gene            complement(3235..3777)
                     /locus_tag="VW077_001629"
     CDS             complement(3235..3777)
                     /locus_tag="VW077_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9455618.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            3956..4723
                     /locus_tag="VW077_001630"
     CDS             3956..4723
                     /locus_tag="VW077_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEU9455619.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLNKLVSGSNTLTQKSSRLTAGVG"
     gene            4808..5488
                     /locus_tag="VW077_001631"
     CDS             4808..5488
                     /locus_tag="VW077_001631"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9455620.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVLVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
     gene            complement(5589..8141)
                     /locus_tag="VW077_001632"
     CDS             complement(5589..8141)
                     /locus_tag="VW077_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9455621.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTIPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(8203..9825)
                     /locus_tag="VW077_001633"
     CDS             complement(8203..9825)
                     /locus_tag="VW077_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9455622.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEDDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         3301 a         1523 c         1828 g         3201 t
ORIGIN      
        1 gaatagagtc atgaaaatta aagttgtaac agttgggaaa ctgaaagaaa agtatttaaa
       61 agatggtatc gcagagtatt caaaacgaat ttctagattt gctaagtttg aaatgattga
      121 gttatcagat gaaaaaacac cagataaggc cagtgaatca gaaaatcaaa agattttaga
      181 aatagaaggt cagagaattt tatcaaaaat tgctgaccgt gatttcgtta ttgtgttagc
      241 cattgaaggg aaaactttct tctcagaaga atttagtaag cagttagaag aaacttctat
      301 aaaaggattt tctactctta cttttattat tgggggaagt ttaggattgt catcatctgt
      361 aaaaaataga gccaatcttt ctgtcagttt tggtcgccta accttacccc atcagttaat
      421 gagactagtt cttgttgaac aaatctatcg cgcttttacg attcagcagg gattccccta
      481 ccataaatag aaaattgact tttaattgaa tttttggtag aataattgta ttaggtctca
      541 tagctcagct ggatagagca ttcgccttct aagcgaacgg tcgcaggttc gaatcctgct
      601 gggatcaata taatagcaaa gctgggaatt ttcccggctt ttttcttaaa aaagtacact
      661 ttgggagaaa aaaatgacag ttgagagaat tttatctaaa acgaaattcc attttgtata
      721 atggtttttg taagttagct tacaagaaaa aacattttag gagattttat tatgaaaaac
      781 acagttaaat tggaacagtt tgtagctttg aaggaaaaag acttacaaaa gattaaaggt
      841 ggggagatga ggttgtcaaa attcttccgt gattttattt tacaaagaaa aaagtaatga
      901 aataagggga aagagtaatg gatttatttg gatttgggac ggttattgtt cattttttaa
      961 ttattagtca cagttaccat tttatttgta aaggtcaaat aaatagaaaa gaattattcg
     1021 tttttggtgc ttatacatta ctaacagaaa tagtatttga ttttccctta tatattctat
     1081 atttagatgg gttagggatt gaaagatttt tatttccttt gggattatat tcctattttc
     1141 gatggatgaa acagtatgag agggatagag gactattcct aagtttacta ctatctcttt
     1201 tatatgagag cactcataac tttctgtccg taactttctc ctctataaca ggagataatt
     1261 ttgttttaca atatcatttc ccattctttt tcgttgtgac ggtgttaacc tattttgtta
     1321 cattaaaaat catttactat ttccatttgg aactagccta ttttgacgag gactaccttt
     1381 atcctttctt gaaaaaagta ttttttgctt tactattgct acatattgta tctttcgttt
     1441 cagatatggt aagtacgatt aaacatttga atagttttgg aagtattttg tcatctattg
     1501 tctttatctc tctccttttg accttctttg caatgaattc tcataaagtt caaatggaga
     1561 aagagattgc tttgaagcag aagaaatttg aacagaaaca tttacagaat tacacagatg
     1621 aaattgttgg tctgtataat gaaatccgtg gttttcgaca tgattatgct ggaatgcttg
     1681 tcagcatgca gatggcaatt gacagtggta atttacagga aattgacaga atttacaatg
     1741 aagttttagt caaagcaaat cataaattgc gttcagataa gtacacttac tttgatttga
     1801 acaacataga agactcagct ttacgaagtt tggttgctca gtcaattgtc tatgctcgaa
     1861 ataatggtgt agagtttaca ctggaagtaa aagatacgat taccaagctt ccaattgaac
     1921 tattggattt ggttcgtatc atgagcgttt tattgaataa tgctgtcgaa ggatcggctg
     1981 atagctataa aaagcagatg gaagtagcag ttattaagat ggaaactgaa acagttattg
     2041 tgattcagaa ttcatgtaaa atgacgatga ctccttcagg agatctattt gccttaggat
     2101 tctccactaa gggaagaaat cgcggagtcg gattaaataa tgtgaaagaa ctactagata
     2161 agtacaacaa tattatttta gaaacagaga tggaaggcag tacatttaga caaatcatta
     2221 gatttaagag ggaatttgaa tgaaagtttt aattttagaa gatgttattg aacatcaagt
     2281 gagactagag agaatattgg atgaaatttc gaaagaatcg aatattccaa tatcatacaa
     2341 gacaacggga aaagtccgtg aatttgaaga atacattgaa aatgatgaag taaatcagct
     2401 ttatttccta gatatcgata ttcatggaat tgagaaaaag ggatttgaag tggctcagct
     2461 cattcgtcat tacaatcctt acgctattat cgtctttatc actagtcgat cagagtttgc
     2521 gactctaacc tataaatacc aggtatcagc cctagatttt gttgataagg atatcaatga
     2581 tgagatgttt aagaagagaa ttgagcaaaa tatcttctac acgaagagta tgttacttga
     2641 aaatgaagat gttgtagatt atttcgacta caattacaaa ggaaatgatt taaaaattcc
     2701 ttaccatgat attttgtata ttgaaacaac aggggtctct cataaattgc gcattattgg
     2761 taagaatttt gcaaaagagt tttatggtac catgacagat attcaggaaa aggacaaaca
     2821 tactcagcga ttttattctc ctcacaagtc atttttggta aatataggca atatcagaga
     2881 aattgatcga aaaaacttag aaattgtttt ctatgaagac catcgttgtc ctatttcaag
     2941 attaaaaatt agaaaattaa aagatatttt agagaaaaaa tctcaaaagt gattgacaat
     3001 tagcaagaaa ttgatataat ggttatatct gctacagata gaaggtccgt tggtcaaggg
     3061 gttaagacac cgccttttca cggcggtaac acgggttcga atcccgtacg gactatttta
     3121 tccgccatag ctcagttggt agtagcgcat gactgttaat catgatgtcg taggttcgag
     3181 tcctactggc ggagtataga taaaagggaa cacagctgtg ttcctctttt tgtatcaatt
     3241 tgtatcacca agcattttca taaggaagtc tgttatttct tgagaacttt ctttttttcc
     3301 atgtgcaatc caagtttggc agacaccaaa aagtgcatga gttagataga tgctactata
     3361 ttctaattcg gtggtattta gattcagttg cataaatcgc ttttgtaaat ctgtactaag
     3421 catgatatga agtttatttc gtaagaaatt ttggatttct ttagtcccat tttcagaaag
     3481 aagggcagcc agaagtggtt ctgactctag atattcaaaa acttctaaaa tagcgtctct
     3541 tttgtgatga gcatgttttt gaaaaatata ttcaaatgta tggaatagct tgctttgata
     3601 gtgctcaatc atatcatact tatccttata gtgagtatag aagctggaac gactaattcc
     3661 ggctttttct actaatttga cagtagaaat tttatcaaat ggctgttcca tcagtaattg
     3721 taccatagca ttttcaatag ttcgctttgt ttttaagcgt ttgttacttt cttgcatatt
     3781 tcctccttgt aaacaaatta gactatatgt ctaaaaatag attttttatc ttgtaattta
     3841 gattttttaa tgtataatct attatatcaa aattttagac aatatgttta aaaaaggaga
     3901 aactaagttt aaagaatgga aagcaattta aaaaaaacca acctttatta ttgtcatgat
     3961 cgggatttct cttattccag atctgtacaa tatcatattt ttgtcatcaa tgtgggatcc
     4021 atatgggcaa ttgtctgact tacctgtggc agttgtaaat aatgataaag aggcttccta
     4081 taatggtaat actatggcaa taggaaaaga catggtgtcc aatttaaaag aaaataaaac
     4141 cttggatttt cattttgtag atgaagagga aggaaagaag ggattggaag atggcgatta
     4201 ctatatggta gtgactttac caagtgattt atctgaaaaa acaactacat tatccaatat
     4261 tcaatcgaca gcagcttatc aatcattgac aagtgagcaa caaactgaga taagtgattc
     4321 tgtatctcaa aattcaactg atagtattca atcggctcag tcaattgtag ctttagtaca
     4381 agatttacag ggaagtttag aaaacttaca aaatcaatct tctaatcttt cgactttaaa
     4441 aaatcaatct aatcaagtat cacctattac ttctacttct ttgataggat tgtcaagtgg
     4501 attaacagag atacaaggag atgttactag caaattagtt cctgccagtc agtcgattgc
     4561 atcaggtgta aacgcatata ctacaggtgt tgataaagtt tctcagggcg caagtcaact
     4621 aagtgaaaaa aatgccacct tgacaggtag tttgaataaa ctagtttcag gctcaaacac
     4681 cttgacacaa aaatcttcta gattgacagc aggagttggt taattacaat caggatctgg
     4741 gtaattagca gacaaatcca gtcagttact ttcaggtgct tctccattag agaatagagc
     4801 taataaattg gcagatggat ctgggaaact agcagaaggt ggaacaaagt taacttctgg
     4861 attggaagat ttacagacag gacttgcttc tttaggacaa ggactaggta atgctagtga
     4921 tcaactcaaa tcagtatcaa cagaatctaa aaatgcagag attttgtcaa atccactcaa
     4981 tctttcaaaa acagacaatg atcaagttct tgtaaatgga atcgcaatag ctccttatat
     5041 gatatcagtt gctctttttg ttgcagcaat atcaacaaat atgatatttg cgaaattgcc
     5101 ttcaggacgt catccagaga gccgttgggc ttggttgaaa tctcgagctg aaataaatgg
     5161 tattatagct gttttggcag gaattttggt atatggagga gttcagctta ttggtttaac
     5221 tgctaatcat gagatgagaa tatttattct catcatccta acaagtttag tattcatgtc
     5281 tatggtgacc actttaacaa cgtggaatag ccgtatagga gcttttttct cacttatttt
     5341 gcttttacta cagttagcat caagtgcagg tacttatcca cttgctttga caaatgattt
     5401 ctttagatct attaatccct ggttaccaat gagctattca gtttcgggat tacgacaaac
     5461 aatctccatc aacaagtcat tttcctagct gtcatactag ctctatttac tggtttaggt
     5521 atgctagcct atcaacataa gaaaatagaa gaagattaaa aaaatcgacc gattaactgg
     5581 tcgatttttt atgccttaga tgactttcgt ctgtgattat agattccaaa tagtaagaga
     5641 gaagtaaagg aacagattgc tccagtaata aaaccattgg gaatgaagga aagtgtaata
     5701 gttcctttcc ccttgggaat gtcaactttc ataaatccag tttgagcttg tttaatttct
     5761 attttcttac catcttggta ggcagaccaa cctttgtcat aaggaatggt gaagaaaata
     5821 gatgtatctt gttggacatc atatgtagca aaaaccttgt ttttagaagt tgatactgtg
     5881 acaggttgtt ctttaatttt ttgaattgcc tcggtgaaag ttttggtatc taaacgatag
     5941 aaggtaggag attcaaatga tacttgtgaa tttccaggga aactaacatt gatattgaaa
     6001 gtttttttct ctttagtata tcctagatta aagaaggaga agacattatc agttgtaaaa
     6061 gtcttttttt caccatttac aaggatgtca accttctttt gtttatcgtt agaaaagtga
     6121 aggtttatga aagagagata aacttggctg ttttctggaa cttcaatttg atactggatt
     6181 gctgcatctt catttgaaga acttgtgaca ctaatcaaat cattagtatt ttctattttt
     6241 tctgtttttt cataaggtat tggagaaaaa taatcaaaat tgacgttagc aagttgattt
     6301 aaaaatgagg cctgattatc caaggtatgt tcattgaact tgacatcatt gtaaacagat
     6361 tgactagcaa ctgcaatcgg aagagagtat tgattttcat atagggtaag attatctttt
     6421 tgatagatat ctttaaagcc atacttatca ataggactgt ctgagatatt gtactggata
     6481 ccaaataaac tatcagccaa aatactatta tttgcatatc ggagattgag attagtccca
     6541 gaggatttaa aaccaagttt atctaaagta gagcttgatg aacgatttcg aacagatgaa
     6601 aattgagaga ttccattgta gttgaatttc atactgtcat ttcctgtctg agtttgtagt
     6661 ttttcagtac gagtaaattg atttccaata tatgttgaga aagattccat agctgggata
     6721 tctcgactat aagcacttcg agaagcaaat ccccattcct tagcaattcc gtccatttga
     6781 gatgaagcat ttaaactcat ttcaaccagt ataaataaag agattagaat ggcaaataga
     6841 ttcacagata taaacttttt gataactgca aggagtaaaa gagaatagac aaccaaaaat
     6901 tcaagagtaa gcagaatatt caaatctgtt aaaaaagaat aatgcgattt tagatagatg
     6961 gtagctaaaa atcctgttac tacaagaaaa agcgaaacta aaaaattcca gactttaagt
     7021 tctttcagac gctttaagac ttctgctgct gtgtaaatta acaaggtaga gaaaatccaa
     7081 gcatagcgat gtaaaaacat gtttggagta tgcatgcctt gccaaaataa gtcaagagct
     7141 tctatgtaaa agcttgcaat tagaaatgca aagaatatta catatatgag tttcacgtga
     7201 aacttaatag atttcagcgt aaaaaataaa atggtcaaaa taaagggaaa tagtccaaca
     7261 aaaatcattg ggatggcccc atactttgtt gtgtcaaagg aaccaatgaa ttgcttagca
     7321 aagagatcaa gataccagct actttcagtt tgaaactttg taacttcagt caatttttcc
     7381 ccatgtgtct gtaaatcaaa tagagtggga atagtcataa tcaaactagc cataccagct
     7441 aaaaaggaga taactatgaa atcaagaaca gatgattttc gagtcttaaa gtcccacgaa
     7501 atttgacaga gataccagaa aataagaaac aatactgtca tatatccaaa ataataattt
     7561 tgaataaata agattgacag acttgtaaag tacaatagga gtttcttttc agttatcagt
     7621 agatgtaaac cagttataat taaaggaatc aagataaaaa catctagcca ggtttttatc
     7681 tctaattgac tgacagtgaa actcatcaga gcataggaag tagataaggc tagttttaaa
     7741 gtctgaggga tagattgaaa caatttattc aaactaaaaa aggttgacag accaatcaat
     7801 ccaaatttta agagagtggt cagatagaca gcatctggca tattcgttag atcaaaaaag
     7861 taaactagag gcgcgagaaa actacccaag taataactag atagggcata gaagtttagc
     7921 cctagaccac ttgtaaaggt gtaaaacaga ctactatttc catgtaggat atttcgtaag
     7981 gctacatcaa aaataacgta ttgatgaaag ccatctccta atagaggaga gttgtcgcta
     8041 ttccagtaga taccttgaga tagatatact ccagacataa tcactacagg aatgatgaaa
     8101 gaaataaaat aggttcgata tgtttttaaa aatgatttca tgttacctcg tagaatgata
     8161 gaaaactcag ttggttaacc caactgagtt ttgaagtttt atttagtctt tccaaagttc
     8221 tttaactttt gcttgtactt ctgcattttc taggaattca tcgtaggttt catcgatacg
     8281 gtcaatgacg ccatttttag ataagacaat gatatggtta gccaaagttt gaataaactc
     8341 gtggtcatgg ctggcaaaga tgattgattc tttaaagttt ttcaatccat cattcaagct
     8401 tgagatagat tccaagtcca agtgatttgt tggatcatca agtacaagga catttgattt
     8461 taagagcatg agttttgaaa gcatgacacg aactttttct ccccctgaca agacatttac
     8521 aggtttgtta acttcatctc cagagaagag catacggccg aggaaaccac gtaggaaagt
     8581 attgtcatca tcttctttac ttgcgaattg acgcaaccag tcaagaattg attctcctcc
     8641 tgcaaaatca gctgagttat cttttggtag gtaagattga ctagttgtaa ctccccactt
     8701 gacagttcct tcatagtcaa tatctcccat gattgcacga attaatgcag tcgtttgaat
     8761 atcattttgt ccaataagtg ctgtcttatc atctggacgc aagatgaaac taatattatc
     8821 caagatagtt tcaccatcaa tctttacagt tagattttct actgtcaaga gatcattacc
     8881 aatctcacgt tccgctttaa agttgataaa tggatattta cgactagatg gtacaatctc
     8941 ttctagctca atcttatcaa gcattttctt acgtgatgtt gcctgccttg acttagaagc
     9001 attggcagag aaacgagcaa cgaattcttg caattgttta attttttctt ctgctttagc
     9061 attacggtct gctagcaatt tagcagcaag ctcagaagat tccttccaga agtcgtagtt
     9121 tccgacatag agtttgattt ttccaaagtc aaggtcggcc atgtgagtac aaactttgtt
     9181 taagaagtga cggtcgtggg atactacgat aactgtgtta tcaaagtcaa ttaagaagtc
     9241 ttctaaccaa gtaatcgatt ggatatccaa accgttagta ggctcgtcca agagaagaac
     9301 atctggttta ccaaaaagtg ctttggcgag gagaaccttt actttttcac cgttggccaa
     9361 ttcgctcatg ttttggtagt gtaattcttc tggaatgttt aggttttgaa gtagttgaga
     9421 ggcttcactc tctgcttccc aacctccaag ttcggcaaac tctccttcga gttcggcagc
     9481 acgaaccccg tcctcgtctg agaaatcttc cttcatgtag atagcatctt tctctttcat
     9541 gatgctataa agtttttcat ttcccatgat aacgacatca atggcacgtt catcttcgta
     9601 gtcaaagtga ttttgacgaa gaacagagag acgttcatct ggaccaagag agatgtgacc
     9661 agtagtaggt tcgatatctc cagctaaaat ttttaaaaag gttgattttc cggcaccatt
     9721 agcaccgatt aatccgtaag tatttccttc tgtaaatttg atattgacat catcaaaaag
     9781 tttgcgatca ctaaaacgta gtgaaacatc agatactgta agcaatattt ttctcctata
     9841 tgtgtaatat att
//
LOCUS       DAVKGU010000074         9829 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKGU010000074 DAVKGU010000000
VERSION     DAVKGU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.074"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(203..1873)
                     /locus_tag="VW077_001634"
     CDS             complement(203..1873)
                     /locus_tag="VW077_001634"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9455623.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCALIDVPVELASVWADGVEGGVELAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQVVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2103..2792
                     /gene="coaB"
                     /locus_tag="VW077_001635"
     CDS             2103..2792
                     /gene="coaB"
                     /locus_tag="VW077_001635"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9455624.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2804..3355
                     /gene="coaC"
                     /locus_tag="VW077_001636"
     CDS             2804..3355
                     /gene="coaC"
                     /locus_tag="VW077_001636"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9455625.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3339..3902
                     /locus_tag="VW077_001637"
     CDS             3339..3902
                     /locus_tag="VW077_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9455626.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            4184..4711
                     /locus_tag="VW077_001638"
     CDS             4184..4711
                     /locus_tag="VW077_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9455627.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            4723..5238
                     /locus_tag="VW077_001639"
     CDS             4723..5238
                     /locus_tag="VW077_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9455628.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(5235..5702)
                     /locus_tag="VW077_001640"
     CDS             complement(5235..5702)
                     /locus_tag="VW077_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9455629.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFTGTLQS
                     SEEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(5775..6263)
                     /locus_tag="VW077_001641"
     CDS             complement(5775..6263)
                     /locus_tag="VW077_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455630.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIDDLKVVGFISYGSFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LVKVALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(6229..6792)
                     /locus_tag="VW077_001642"
     CDS             complement(6229..6792)
                     /locus_tag="VW077_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455631.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(6802..8790)
                     /gene="uvrB"
                     /locus_tag="VW077_001643"
     CDS             complement(6802..8790)
                     /gene="uvrB"
                     /locus_tag="VW077_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9455632.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMTYNEEHDIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKVLD"
     gene            complement(8867..9820)
                     /locus_tag="VW077_001644"
     CDS             complement(8867..9820)
                     /locus_tag="VW077_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9455633.1"
                     /translation="MKNKRIFKDFQASKMNLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         2923 a         2205 c         1764 g         2937 t
ORIGIN      
        1 agccgcaggt tgctcaaaac actgttttga ggttgtggat agaactgaca gagtcagtat
       61 catataccta cggcaaggtg aagctgacgt agtttgaaga gattttcgaa gagtataaat
      121 cttattgatg aactgcttgc agtctgagaa aaaatgagct tggatattat ttccaaactc
      181 acttaaagtc aatttcaatc cactagaaca agcctagtac agttccatcg ctttcaacat
      241 ccatgttgag agctgctgga cgttttggaa gacctggcat ggtcataaca tcaccagtta
      301 aggcaacgat gaagcctgca cctaattttg gtaccaattc acgaatggta atttcaaagt
      361 tttctggtgc tccaagcgca tttggattgt ctgagaaact gtattgagtt ttagccatac
      421 agattggcaa tttgtcccaa ccgttttgaa cgacttgagc aatttgtgtt tgagctttct
      481 tctcaaagtt cactttgcta ccacgataga tttcagtgac aattttttca atcttttctt
      541 ggacagaaag gtcattatca tacaaacgtt tatagttagc tggattttca gcaattgtct
      601 taacaactgt ttcggcaagt tctactccac cttctactcc atcagcccag acactagcca
      661 attcaactgg tacatcgatt aaggcacaga gttcttttaa ggctgcaatt tcagcttctg
      721 tatcagatac aaattcgtta atagctacaa ctgctggaat accgaactta cggatatttt
      781 caacgtggcg tttcaagtta gcaaaacctg cacgaactgc ctctacattt tcttcagtca
      841 gagcatcttt agccacacca ccattcatct taagggcacg aagggttgcg acaataacaa
      901 ctgcatctgg agatgttggc aagtttggtg tcttgatatc aaggaatttc tcagcaccaa
      961 ggtccgcacc aaaaccagct tcagtaacag tgtaatcagc caagtgaagg gctgttgtcg
     1021 tcgccaaaac agagttacag ccatgagcga tattggcaaa tggaccaccg tgtacaaagg
     1081 caggtgtacc gtaaattgtc tgaaccaagt ttggcttaat agcatccttc aaaatcaaag
     1141 ccaaggcacc ctcaacctgc aaatcaccta cagaaacagg cgtacggtca tagcgataac
     1201 caataacgat attcgccaaa cgacgtttca agtcctcgat gtccgttgcc aagcaaagaa
     1261 ttgccatgat ttctgaagca actgtaatat caaaaccatc ctcacgtgga ataccgttta
     1321 gaggaccacc aagaccaaca gtcacatggc ggagcgcacg gtcgttcaag tccacaacgc
     1381 gtttccagag gatacgacgt tgatcaattc ccagctcatt cccttggtgc aagtggttgt
     1441 caatcaaggc agaaagggca ttgttggcag ttgtaatagc atgcatatct ccagtaaagt
     1501 ggaggttgat gtcttccatt ggcagaactt gtgcataccc accaccagca gcaccaccct
     1561 tgatccccat gactggacca agagacggtt cgcggatagc aatcatggtt ttcttgccaa
     1621 tcttgttcaa ggcatccgca agaccaatgg taagcgtaga ctttccttca cctgcaggtg
     1681 ttgggttgat ggcagtaacc aagatcaatt taccgactgg attgctctca actgcccgaa
     1741 ttttatcaaa gctgagttta gccttgtact ttccgtacaa ctccaaatcg tcgtaagaaa
     1801 taccaagttt ctctacaaca tcaacaattg gcttcaactc aatactctgt gcgatttcaa
     1861 tatctgtttt cattcaaaat tcctctaacc tcttatatga taattcatta tatcacaaaa
     1921 caagattttt aacatcctaa aactctctaa acgttcgtaa atatctctgt ttttaaggct
     1981 tttagagtcc tttcttaaat tttatatggc tttatagttt gaaactataa taaatcttcg
     2041 tttttaccaa aaatttatca ctttcatttt acttaccgct tatttttgtg tacaatagtg
     2101 ctatgaaaat tttagttaca tcgggcggta ccagtgaagc tatcgatagc gtccgctcta
     2161 tcactaacca ttctacaggt cacttgggga aaattatcac agagactttg ctttctgcag
     2221 ggcatgaagt ttgtttaatt acgacaaaac gagctctgaa gccagagcct catcctaacc
     2281 taagtattcg agaaattacc aataccaagg accttctaat agaaatgcaa gaacgtgttc
     2341 aggattatca ggtcttgatc cactcaatgg ctgtttctga ctacactcct gtttatatga
     2401 cagggcttga ggaagttcag gctagctcca atctaaaaga atttttaagc aagcagaatc
     2461 atcaggccaa gatttcttca actgatgagg ttcaggtttt gttccttaaa aagacaccca
     2521 aaatcatatc cctagtcaag gaatggaatc ctactattca tctgattggt ttcaaactgc
     2581 tggttgatgt taccgaagat catctggttg acattgcacg aaaaagtctt atcaagaatc
     2641 aagcagattt aatcatcgcg aatgacctga ctcaaatttc agcagatcag caccgagcta
     2701 tatttgttga gaaaaatcag cttcaaacag tccagactaa agaagaaatt gcagaactcc
     2761 tccttgaaaa aattcaagcc tatcattctt agaaaggaaa actatggcaa acattctctt
     2821 ggctgtaacg ggttcaatcg cctcttataa gtcggcagat ttagtcagtt ctctaaaaaa
     2881 acaaggccat caagtcactg tcttaatgac tcaggctgct acagagttta tccaaccttt
     2941 gacactacag gtactctcac agaatcctgt ccacttggat gtcatgaagg aaccctatcc
     3001 tgatcaggtc aatcatatcg aacttggaaa aaaagcagat ttatttatcg tggtacctgc
     3061 aactgctaac actattgcaa aactagctca cggatttgcg gacaacatgg taaccagtac
     3121 agctctagcc ctaccaagtc atattcccaa actaatagct cctgctatga atacaaaaat
     3181 gtatgaccat ccagtaactc agaataatct gaaaacatta gaaacctacg gctatcagct
     3241 gattgctcct aaggaatccc tactagcttg tggagaccac ggacgaggag ctttagctga
     3301 cctcacaatt attttagaaa gaataaagga aactatcgat gaaaaaacgc tctaatattg
     3361 cacccattgc tatctttttt gctaccatgc tcgtgataca ctttctgagc tcacttatct
     3421 ttaacctttt tccatttcca atcaaaccga ccattgttca tattcctgtc attattgcca
     3481 gcattattta tggtccacga gttggggtta cacttggatt tttgatggga ttacttagct
     3541 tgacggttaa cacgattacg attctaccga caagctacct cttctctccc ttcgtaccaa
     3601 acggaaacat ctactcagct atcattgcca tcgtcccacg tattttgatt ggtttaactc
     3661 cttacttagt ctataaactg atgaaaaaca agactggtct gattttagct ggagcccttg
     3721 gttccttgac aaatactatc tttgtccttg gaggaatctt cttcctattt ggaaatgttt
     3781 ataatggaaa tatccaactt cttctggcaa ccgttatctc aacaaattca attgctgaat
     3841 tggtcatttc tgcaattcta accctagcca ttgttccacg actacaaacc ttgaaaaaat
     3901 aaaaacaatc tccgctttca aacctgaagg tggagatttt gctctataca gtttcttttt
     3961 aatgaaatct ttactatagt aaaacgaaat aagaacagga taaatcgatc aggacagcca
     4021 aatcgatttc taacaatgtt ttagaagtag aggtggacta ttctagtttc aatctactat
     4081 aacttttaac taaaaaatta tctataaacc caagtaaatt cttgctttat tgtctggttt
     4141 attgtaccat actagtgtat atgcagttaa aaaggagatt cttgtgaata cacggaaaaa
     4201 gacacaattt atgacaatga cagccctttt aacggctatt gcgattttga ttccaattgt
     4261 tatgcctttc aagattgtca ttccacctgc ttcctatact ttggggagcc acatcgctat
     4321 ttttatagcc atgttcttgt cgcccttgat ggcagttttt gtcatcctag cctctagttt
     4381 tggatttttg atggctggct atcccatggt tatcgttttt cgggcttttt cccatatatc
     4441 ttttggtgct ttaggagctc tttacctaca aaaattcccc gataccctag ataaaccaaa
     4501 atcttcctgg gttttcaact ttgttttggc tgttgttcat gcccttgctg aagtattagc
     4561 ctgtgtcctt ttttacgcaa cttcgggtac caatgtagaa aacatgttct atgttctatt
     4621 tgtgctagtt ggatttggca caattatcca tagtatggta gactatacat tagcactagc
     4681 tgtctataaa gtgcttcgaa aacgccgtta aaaggaaaag ctatgacaaa agatcgcaaa
     4741 caagccctgc tccaactctt gaaagaagct cctaaagccc ttaatggcca aagtttggcg
     4801 gaacattttc atgtcacacg tcaggtcatt gtgcaggaca ttgcaatttt gagagccgat
     4861 ggcgctccta tcctatccac caatcgtggc tacatctata agcaaattga taccaatcct
     4921 tatgttcaca aacttttcaa agtgaaacat gaagttgaag aaatcggtca ggaactcctt
     4981 gctattgtcg ataatggtgg gcatgtccag aataccttga ttgaccatcc cgtttatgga
     5041 gaaattgaaa ccttgcttaa actgtcttgt cgcagagatg tgcaacattt tctagaacaa
     5101 gtcgagcatt ctgactttag acctctatct gaattgacag atggcatcca ttaccaccta
     5161 gtcgaagccg aaacacaaca agacctccac tatatcgagg aggccttgga tcagctaggt
     5221 tatttagtaa aagactagaa gattttcttt tcccaatcgt cttctgtacg gcgagggtag
     5281 aaaaattcag acttatcagg agcttccatc atctccatca agggtagcat atcataggcc
     5341 agatccaagt ttggaatctg gtctttttgc acccaagaaa cttctccctc ttctgaagat
     5401 tgaagagttc cagtaaactc agttgcctta taacaaatga caatatagcg cccacctgta
     5461 tccagtggcc aatttttaat gccgacaagt tgaggatttt ggatagtcaa ccctgtttct
     5521 tcatagattt cacgaatgac agactctgcg aaagactcgc cattttctac atggcctcct
     5581 ggaaaggcat aaccagacca gcgattgttt tcaggagcgc gatactgcat caccacgcgc
     5641 tgagtttcga ggtcttcaat cagacaaatg tttgttaaaa tcgttaattg tgaacgggac
     5701 ataaatttac tcgctttcta atttattaaa ttttcagact tggtaccaga gatattagct
     5761 tttacttttt aaaattattt agaatagaat accatccgtt tttcctttat aggctttcca
     5821 agttcaagta tcttttcttg tccatcaaaa gtaaaaccca ttttttgata gaaagcaatg
     5881 gcgcgcttat tatctttcaa tacccataag aaaatttcag aaaaatgatt aagagcagtc
     5941 aaagctactt tcactaactt ttgtgcgatt ccttttccat aatagtcttt taaaacatat
     6001 aaagcaataa tttcaccagc ttgaatagtc tcatcacgaa agctgccata actgataaaa
     6061 ccaactacct tcaggtcatc tatcgcaatc aatgtatttt ctggatactt ttgactaaag
     6121 aatcgacatc tttctaatgt cattgtttcc tgaaattctg caggcaaaag gtcatcataa
     6181 gcctctctcc acgtttgcca gtgaacaagg gatttacctt ctatctcttc aggagtttcc
     6241 attgatttga taataaccgt catttatttt ctcccagtct tctctcaaaa taccatattt
     6301 aatactatca aaatatttac cttgataata acgaactttt ggaatatgag cttctttttt
     6361 cattcttaat ttttcagcaa gtttcatcat accaagattc cctgaccaag ttgtcaaacc
     6421 cagatgctcc aactccaagt aatcctgaaa cgtcctatct atccactgca acatagcaac
     6481 tttcccaata ccagtgttcc agaatttttt atcataaata ccaattccca attccatcca
     6541 tcttgtttgt ttacataccc aataacgcga aacagtccca actagtttat catcaacaaa
     6601 aataccaagg cgatttgagt tgcttaaaat ggattctgat ttttgtagtt cgaattcttt
     6661 aaaatttgaa aaatactgat aatcatcata atagggagca tcatactgtt tccaaactgg
     6721 attaagttgt gaataagcca tttcccataa agacatcaaa tcttctctct ttaactttct
     6781 taaataaatc atactattcc cctaatccaa gaccttgact tccaacatca tatcacgaat
     6841 ctgagccgcc agttcaaagt caagcacttc gacggcttct tgcatttgtt tttctagttt
     6901 cttgacgagt tccttgcgct cttgtttgtt gaggctattg atgtcgactt ccttgtcctc
     6961 ttccttagca actgccttgg tcacggcaat caggtcacgg atttctttct tgattgtctg
     7021 tggtacgata tcatgctctt cattataggt catctgaatt ttccgacggc gggcagtttc
     7081 atcgatagca cgttgcatag actgggtaac cgtgtctgca tacatgatga catggccttc
     7141 actgttacga gcagcacgtc caatagtctg gatgagtcca cgttcgttgc gaaggaaacc
     7201 ttccttatca gcatcgagaa tagctacgag gctcacttca ggaacgtcaa ttccttcacg
     7261 gagcaggtta attccgacca agacatcaaa gacacccaag cgcaggtcac ggataatctc
     7321 cgtccgttcc aaggtcttga tatccgagtg catgtacttg accttgatac ccatttcctt
     7381 gaagtagtcg gtcaagtcct ctgccatttt cttggtcaaa gttgtgataa aggtacgctc
     7441 atttttttca acgcgggcat tgatttcacc caagaggtca tcaatctgtc ccatagtcgg
     7501 acggacttcc acttcaggat ccaagagtcc cgttggacga atgatttgct caatcactgt
     7561 ctcagtctgt tcattttcat agtcaccagg tgtcgctgaa acgtaaacaa tctgatgaac
     7621 gtgactctca aactcctccc gacggagagg acgattgtcc aaagcagacg gcaatcggaa
     7681 gccataatta accagcattt ctttacgcga acggtctcca ttgtacatgc ccttgatttg
     7741 ccccatggtc atgtgactct cgtcaatcat aattaagaaa tcatctggga agaaatctaa
     7801 gagcgtataa ggaggctctc cttcgctccg tccatccatg tgtcgtgaat agttctcaac
     7861 cccattggta tagcccatct cacgcaacat ttcgatatca tactctgtcc gctgtttcaa
     7921 acgctgggct tcaagcagtt taccttcctt ttcaaagaca gctaattgtt cttccaactc
     7981 ggcctgaatc tttgcaatgg caacttccat gtggtcgtca ttggtcacaa agtgtgtcgc
     8041 tgggaaaatc gctaaatgat ccacttctcc caacacctga cctgtcagag cctcaacttc
     8101 acgaatacgg tcaatttcgt ctccaaaaaa ttctactcga aaggcatgtt catctcggga
     8161 agctgggaaa atctctacca catccccacg aacgcgaaat cttccgcgtt ggaaatcaat
     8221 atcattacgt tcaaactgaa tatcgaccaa gtcattcaag agtttatcac gagaaatctc
     8281 tagaccagga cggagactaa cgacactatc agcgtattcc ttgggcgaac ccaaaccata
     8341 gatacaagag actgaggcca cgacaataac atcattacgc tccaaaaggg ctgaggtagc
     8401 tgagtggcga agcttgtcaa tctcgtcatt gacagaacta tccttctcaa tataggtatc
     8461 gctagaaggg acataggcct ctggctggta ataatcatag taggatacga aatactcaac
     8521 tgcattttca gggaaaaatt ccttaaactc cccatagagc tgaccagcca gagttttatt
     8581 gtgggcaata accagagttg gtttattgac tttagaaatg acctgactca tagtataggt
     8641 cttccctgtt ccagtcgccc ccatcagaat ctgagctttt tctcccccct caatgttatc
     8701 caccaactgc tcgatagctt ggggttgatc tcctgatggt tgatattttg atactagttt
     8761 aaattgatta tctgtaatgt gattgatcat aagaatcctc tctccatgtg ctattcctat
     8821 tttagcatac ttatagtatt ttcacgaaga aaaggacgga ccgaagtcac atcctttcat
     8881 tttctttctt taatttatta gcaagataga caatcagcag tagtaatacc agacttgtca
     8941 taatggaacc ttctatgcca aaagaaccac ctgatagcca atcttgattg ccttgtggta
     9001 aaaaggtcat cagagacgtt cctgacggtt gaccactaac taaaatccca aagagattac
     9061 cctgagcaaa attccaagca ccatgaatac ctgcaacacc ccaaactgta tcagttttga
     9121 gaaggtaaag agccatggca actccgaata aaaagagatt tactagagat agaggggtga
     9181 gaccagaatt gcccatatga agcagggtaa agaacaggct agatataaga atagctagtt
     9241 ttagattggt tcttgaggcc aattgaggaa gtagccaagc acgggccacc acttcttctg
     9301 ctgtcccctg taaaatccaa aatgggatag taaagacgac aaaggcaaga gaataaggat
     9361 tcaagtgaat ggattccaaa cgatattgac ctaagaccac caaacctaac aaggtcagaa
     9421 gaaaaagtgc cagacctaga ctaaatcctt tcagaagatt gctgaggaaa ttctctctat
     9481 aaaatcccaa ggttcgaata ggtcttttct caacttttct tgtccatctg aacacagtgt
     9541 taagaataaa accaaaggcc agtaatccta aaattaaacg aaagtcactt gttttatccg
     9601 tcaagctctg atgcaaggtc tgcaagtagc ttgcaagatt ttgaccagcc tctccaaaat
     9661 ttctagcaag tccgatgaga gctaacaaac taataccata taaagtataa gccacaaact
     9721 ctcctatgaa gacaaaaaca aaggctaaca aggggcttgt gtaaatgttt aaattcattt
     9781 ttgaagcttg gaagtcttta aatattcttt tgttcttcat atccccgtc
//
LOCUS       DAVKGU010000075         9807 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKGU010000075 DAVKGU010000000
VERSION     DAVKGU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.075"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     regulatory      110..208
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            292..1083
                     /gene="thiD"
                     /locus_tag="VW077_001645"
     CDS             292..1083
                     /gene="thiD"
                     /locus_tag="VW077_001645"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9455634.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIHPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(1293..1925)
                     /locus_tag="VW077_001646"
     CDS             complement(1293..1925)
                     /locus_tag="VW077_001646"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9455635.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMDTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(1918..2721)
                     /locus_tag="VW077_001647"
     CDS             complement(1918..2721)
                     /locus_tag="VW077_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9455636.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAALYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(2721..3245)
                     /gene="thiW"
                     /locus_tag="VW077_001648"
     CDS             complement(2721..3245)
                     /gene="thiW"
                     /locus_tag="VW077_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9455637.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            complement(3250..3942)
                     /gene="tenA"
                     /locus_tag="VW077_001649"
     CDS             complement(3250..3942)
                     /gene="tenA"
                     /locus_tag="VW077_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9455638.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKVFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(3953..4603)
                     /locus_tag="VW077_001650"
     CDS             complement(3953..4603)
                     /locus_tag="VW077_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9455639.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMFY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(4605..5990)
                     /locus_tag="VW077_001651"
     CDS             complement(4605..5990)
                     /locus_tag="VW077_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455640.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDYMVELRDGKLREVFQIPS
                     YEMTQVASKEVAASPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(5991..6551)
                     /locus_tag="VW077_001652"
     CDS             complement(5991..6551)
                     /locus_tag="VW077_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9455641.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(6813..6901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(7110..7739)
                     /locus_tag="VW077_001653"
     CDS             complement(7110..7739)
                     /locus_tag="VW077_001653"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9455642.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGVKLMRKLL
                     PQMPLVAIGGIQTKHIKDIMKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(7741..8523)
                     /gene="thiM"
                     /locus_tag="VW077_001654"
     CDS             complement(7741..8523)
                     /gene="thiM"
                     /locus_tag="VW077_001654"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9455643.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVIASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(8766..9434)
                     /locus_tag="VW077_001655"
     CDS             complement(8766..9434)
                     /locus_tag="VW077_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9455644.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(9649..9737)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         3049 a         2124 c         1750 g         2884 t
ORIGIN      
        1 agaaataata ttttcttcga aaaatagaat tttaatcatt tgaaaaacga tttgcagtca
       61 aatattacta tataaacaat aaaaatatgc tatactaaag aaaaaagaaa acaaccacta
      121 ggggtgcgta aagctgagat taacgactgt tagatccctc tgactcaatc taggtaatgc
      181 tagctgatgg aagtggaaat gataatgggg actagcagtc ttctattgcc tttctaaaac
      241 agactagctt gttcttaaga atacaaactt cagttggttg ggaggtttta gatgacttat
      301 ttacccgttg ctttgaccat tgcagggact gaccctagtg gtggtgctgg cattatggca
      361 gatttaaagt cattccaagc gagagatgtc tatggaatgg ctgttgtaac cagtcttgtc
      421 gctcaaaata ccagaggtgt tcagctaatc gagcacgttt ctcctcaaat gttgaaagcc
      481 caattggaga gtgtcttttc tgatattcca cctcaggctg taaaaactgg aatgttggct
      541 actactgaaa tcatggaaat catccacccc tatcttaaaa aactggattg tccctatgtc
      601 cttgatcctg ttatggttgc tacaagtgga gatgccttga ttgactcaag tgctagagac
      661 tatctcaaaa caaacttact acctctagca actattatta cgccaaatct tcctgaagca
      721 gaagagattg ttggtttttc aatccatgac cccgaagaca tgcagcgtgc tggtcgcctg
      781 attttaaaag aatttggtcc tcagtctgtg gttatcaaag gcggacatct caaaggtggt
      841 gctaaagatt tcctctttac caagaatgaa caatttgtct gggaaagccc acgaattcaa
      901 acctgtcaca cccatggtac tggatgtacc tttgctgcag tgattactgc tgaactagcc
      961 aagggcaaga gtctttacca ggcagttgat aaggccaagg cctttatcac aaaagctatt
     1021 caagatgccc ctcaactcgg tcatggttct ggtccagtca accatacaac ttttaaagat
     1081 taagaaaaaa aactctctag ttcccacttt aagggaatta gagagttttt atactcttcg
     1141 aaaatctctt caaactacgt cagcttccat ctgcaacttc aaaacactgt tttgagctga
     1201 cttcgtcagt cttatctaaa acctcaaggc agtactttga gcaacctgcg actagctttc
     1261 tagtttactc tttgattttc attgagtatt aattaggaaa gaatgtcatg caactttgtt
     1321 aaaaaggctt gcgtttttgc ctcaatatct tctgcttgca tcaaatcacg tacaacagct
     1381 acaccagcta tgccagtgtc cataagctga tcaatattct ctgatgtcaa gccgccaata
     1441 gcaactactg gaatggcaac cgtttggcaa attgttttca aggtcgatat cagagtaatg
     1501 ggcgcatttt ccttggtggt ggttgggaaa atggctcctg tacccaagta atctgcacct
     1561 gatttctccg cttccagagc tcttttaacc gttttagcgg tgacaccgag gattttttca
     1621 ggacccaaga ctttgcgagc taccgaaact ggtaattcat catctccgat atgcagacct
     1681 gctgcatcaa ccgcaagaca aacatccaac cgatcatcga ttatcaaggg tacctgataa
     1741 gcatctgtta tttccttgac ttgttttgcc agttgataat attgattggt tgtgagattt
     1801 ttttctcgca attggactat ggtaacccct gaacggcagg ccgtctcaac ttttgcaaga
     1861 aagctttcca cggaatcttg atagcgattg gttaccagat atagtctaag tgcttctcta
     1921 ttcataaacc tctcctttga tggtatctag ccaattttca tctcttctta ggagcgaaag
     1981 ctgattgagt acttggtaac gaaattcttc caatcccatt ccttgaacaa ctattttctc
     2041 agcagcgata ttgagataag agactgctaa gcaagaagct tcaaaaccag tctttccttg
     2101 gctgagaaaa acggctgtta aggctccaac caaatctcct gtccctgtta tccagtctaa
     2161 ttctgcacag ccattctcaa gtacagcaac ttgattctcc gaaacaataa ggtccttggg
     2221 acctgtgact aagaatgaca taccaggata ggtctgacac cagtctttca agacttgaag
     2281 caaatcctcc gtttcttgat ctttagcact cgcatcgacc ccaacgccgt gatgctttaa
     2341 tccaacaaga cttcgaattt ctgacatgtt tcctttaagg accgtaggtc tatagtctaa
     2401 aaggtcttta actaagctct tacgaatgga tgaagtcgtt acgccaaccg catctactac
     2461 catcgggaga gaagattggt ttgcatacaa agctgccatg cggattgctt tttccttctc
     2521 agctgacaaa tgccccaaat tgatgaagag agcctggctt tgcttagtaa aatcaagaac
     2581 ttcacgggga tcatctgcca tgacaggttt gcatcccaga gccaaaatcc catttgccag
     2641 catctcacaa gaaatctcat tggtcataca gtgaatgagg gaactagagc ctataggaaa
     2701 aggatttgtc aatgcctgca tcattctatc ctttcagcaa agaaatatcc ttgcactttt
     2761 ttaaagaatt cctgcttgat taaaaatcta aatgcaataa aggaaatcgc tgtaccaatc
     2821 aaggttgctc cgaaaaatcg aggagtgtag ataaaccaac taagcttagc agccgatcct
     2881 gtaaagagca ccataacagg ataggaaaca atagaaccaa taatacctgt tcccacaatt
     2941 tctcccaagg cagaaaagta aaattttcga ccgtacttat aaaagagacc tgctagaagg
     3001 gctccaaaag tcgctcctgt gagagctaaa ggcggaatcc cttgagtcgt catacggata
     3061 aaggctgtca ctgtagccat agccaaggca taaacaggtc ccatcatgat tcccgctaga
     3121 atattgacta cactggacat cggtgccatt ccctcaatcc gaaagatagg tgtaaggact
     3181 acatcaaggg caatcatcat agataaaatg gtcaatttgt gaacttgtag ttggtgcttt
     3241 ctcatgtttc tattcttctc ctttttctaa agactgtaaa tcgctcttcc atgtctggtg
     3301 ttggtaagcc atctcccaaa acttggcttc catatgaaca ctgatgtgga aggcatctag
     3361 cattttttgc ttatctgtct catcactttc tcgatagagc tgattgacca atgctccctc
     3421 ctctctgatc tgttgctcta actcatccgt aatataagtt tcaatccatt gttgatagag
     3481 aggatttggt gatggtttaa gattaagtga tttgcctata tcatggtata accaaggaca
     3541 aggaagcaag cttgcaaaag ctatggctaa gttcggttct gcaaattgcc tataaatatg
     3601 agaaatgtaa tgataacagg ttggagcgat tggatgttgc tccatttcct ggtcgttgat
     3661 ttccaattcc ttgaaaaatt gttggcgaat aaataactca ccctccacta aaccctgagc
     3721 attttgtttc aagagtcttt tcatctcttg gtttgaagtc ttatcagcca aaagatgata
     3781 gatttctgag aaaaccttca gatagtaggc atcctgaatc aggtaatagc ggaaaatggc
     3841 aggttctaaa ttcccctctt gtaattgtaa aataaaggga tgatgaaagg aagcctgcca
     3901 agctttcttg gataattcca tcgcaatatc tgtaaattcc ataataactc ctttataaaa
     3961 atagactggt ttgaagcaat aaaaagaaaa gcaggtagat taattttgtt tttttaggaa
     4021 tataaaaagt ccgatagcta ttcttcaact gtgcatgttc gtcatatccg tgagcagata
     4081 gagctctcag gtaaagatgg cgccacctaa agactgtcat cagaacctta ctgtaaatca
     4141 agggcgacca aaaatgtagt tcttgaccac gtaataggca agcttctttg agggacttga
     4201 tttcttgctg aatgagagga aaagaattga ataccacaat caaggcatag gaccaagagc
     4261 gtgatagccc cttttgagcc aagtacaaga gaagctcttt tagtgaaata gaggaaacaa
     4321 agacaaggcc gatacaaact gtcacaaagg ccctcgttcc aagcatgact gcctgtgaag
     4381 catctccgtg taactgaact gcccagtagt tggcaaaaga tggtaaaatg gcaagtatga
     4441 tcatccaagc taacatttta aatcgacggt aatagaacat aaagagaata caaaatgcga
     4501 ctaccgaaag attcagagca atcgaaggaa tgaaagatgt ttccaaggat aaaatcagca
     4561 agaagagact gataatcggt gtctgggttg ctactttgac catactatct cacctcccct
     4621 tgggtattgc tactctgaga tgtaagtggt ttggtaatgg tcacttcttt cacatgccga
     4681 agaccctgac tagtcatctc aatccaataa tcaaccacag aaatcaaagg gtctaaacga
     4741 tgactaatga gcagaaaact tcttccttga ttcctctcct ccacaatcca cttgcaaaaa
     4801 taatggcagg ctctatcatc caaacctgca aaaggttcat ctagcaagat cacggaagcc
     4861 ttactggtca agatggtcag gagctgaaga attttttgct gaccaccact taattgatag
     4921 ggactcttat cgactgcctg ctccaaatca aaatatcgta aagcttgaaa aatccgctga
     4981 tttctttcag aatcaggtcc atctaattga agctcctctc gcagactgac tcggataaac
     5041 tgcttctcag cttcctgaac aacacccgtc agatcacggt acaaactctt tttctttttc
     5101 aggactgtcc ccttccatgc aatgcgcccc ttatactttt gaaattgaag aatagaccga
     5161 aagagggttg atttcccgac accattgtca cccaggatac aggaaatccc ttgatagaat
     5221 gtgaaatcag caatcgaaaa gagggggcga ttaccaagct caccagtcac acggttcata
     5281 tggaataatt ccgggctagc agcaacttcc tttgaagcaa cctgtgtcat ctcataggaa
     5341 gggatttgaa acacttccct tagttttccg tctcttagct caaccatata gtcgatatag
     5401 gctttatagt cagataaatc atggtcgcac aaaatgactg tcttcccatc aagagccaac
     5461 tcttttagaa tctccaatat ctcgattcta ctcttgcggt caatggaagc gaagggctca
     5521 tccaagagat agaccctagg attcatggca aagaggacag ccagcgctgc tttttgcttt
     5581 tccccacctg ataagtgatg gatgagacgg tgcaagatgt ccttgcaacg acattgctgg
     5641 acaacctctg ctattttaga atcaatttcc tgaaggtgat agccgatatt ttccatggta
     5701 aaaaccaact cctcaaacaa gctctccatg gtaaattgat gattaggatt ttgcaagaga
     5761 ataccaaccg tctggacacg ttcgacgata gaaagctgac tgacctcgct cccatctatc
     5821 aggacttgac cgctataggg aagagaacta acttgggcaa tgacttgaaa aaggctggat
     5881 tttcctgaac cactgctccc aactaacaag gtaaaggctt gcgcatgaaa agtaaaatca
     5941 aacggctcag agaagattgg ggactgaatc gctcgtagtt ccagacccat ctatgctttt
     6001 cctccagttg caaactgatg atagagtttg acaatggcac gaaccaagat ggtacagaag
     6061 aaataaacag aaataaaacg taccacaagt aaggaaagga caaacggaag ggaaaaggcg
     6121 tagtaaccta acttaatgta ttcatagaca aagctaacaa gcgtaatccc aatactatta
     6181 gcagttagag agagccaact ttcatagcga ttcttagtta cgataaaacc aaattcactt
     6241 cccaaacctt gaactaagcc agacaaaaga gctcctagac caaattggct accataaagg
     6301 acttcagcaa gcgcagctag cacttctcca atcgttgcac ttccgactct cggaacaaag
     6361 atggcagcaa tgggcgcagc catacaccag agaccgaaga ggatttcatt ggcaaaggcc
     6421 tgcaaaccaa gaggtgttaa gagtagactg agaatattat acacatatcc tgaaccaacg
     6481 aaaaccccac caaaaaagat agacaagaaa gcaagcaaga taacatcttt taactgccat
     6541 tttttcaaca taaaaaactc cttttttaaa agaaaagtgg ggcactcaag aagaccgacc
     6601 taaatacttt gtatagcaga ttgaatttag aacagtacac aggaatgcta aaatatttct
     6661 agaaattaat ttgaattttc taattgattt gttcgcatct tatttcaatc tactatatca
     6721 tcttcatcca gtttcgtaaa agaaaaaact ctaattacag atacgaatta gagttcagct
     6781 tacaagatta gacagttctt ttcgacatac gaaaaaaaca tttcacattt cccttcgcca
     6841 gtcttaactg tatcaggttc aatgggtatc atctcagcct aaagcacccc aaatgccttt
     6901 attatttaat tatgtgatta ttataacaca cattttatac tagttcaaga aattgaactg
     6961 gaaatacagc cttgcactca caaagacagc agatctttct tttgcaaaaa acaaatgacc
     7021 tgtttgatga attagctatt caagctgaat ctagacatag ctttttaaaa aaggaaaatc
     7081 ctacttactt agaatccaag gatagatatc tattgttcac tcatttcccg aacagttttt
     7141 tctatatttt ttgcatacga tattgccgaa atgattgaaa cgccgtcaac attggtcttc
     7201 ataatgtctt taatatgttt cgtctgtatc ccaccaattg caactaaggg catttgtggc
     7261 aatagttttc tcatcaattt aacaccttca taacctatag caccaccagc atcatccttt
     7321 gactgggtat caaatacagg accaacacca acataatcta catattcaac ttttgattgt
     7381 tgaaattctt cctcgttttt tatagaaaga ccaattattt tatctggcat caattttcta
     7441 atttcatcaa caccaatatc atcttgacct acatgtacgc catcagcgtc aatttccatt
     7501 gccaaatcga tatcatcatt aacgataaac ggcacattgt attttttaca aagttcttta
     7561 atttggatag ctagttcaag tttttctaag ccttctaaag caccctcacc tttttctcga
     7621 aattgaaata aggttatacc accttttaag gcttcctcaa cgactgtata tagatttttt
     7681 ccttggcaag tagtcgttcc acaaataaaa tatagtttta gtaattcttt atgaaacatc
     7741 ttacttcact cttttgaatt cctttacatc ttcatctgta atctcgtata aggcatttat
     7801 aaattcaact ttaaatgtcc caggaagatg tccatttgga cgtttttctg ccatttctcc
     7861 agcgatattg taaaccaaca ttgctgtttc taatgatttc aattcttgac ctttttctag
     7921 tccgataaag cttgctatca ctgcccctaa taagcatcct gtcccaatga ctttcggcat
     7981 catagcacta ccattatgaa tcgttaccac ttctccatta acagcaatgg catccacttc
     8041 acctgttact actattggaa tattgaactt ctcatttgct gctagagcaa tttcgtcaat
     8101 attatctacg cccgcactat ctactccttt agatgccaca tctattccta ctaaagaggc
     8161 aatctcgcca gcatttcctc taatcgctgc tagtttataa ttgttgatta gatcatctgc
     8221 tacttttttt ctatattctc ctgctccaca ggctacagga tctaaaactg ctgggacatt
     8281 atatttctct gcaattttca gagcagcttg gtataatttc caattttcat ctgtcaatgt
     8341 tcctatgttt attaataaac caccagcata ctttaacaaa tcctctaaat ctgctggaaa
     8401 ctcactcatg gctggtgagg cgcccagtgc tactaatcca tttgctgtga aattttttac
     8461 tacatcattg gttatacaaa tgaccaatgg tgctttttct tttaataatt ttaaacttgt
     8521 catattgaaa tccttccttt tcactttata cgatctactg atttcgattt atttctatct
     8581 ttcgttaaga atttttttca tttacattga atgatttata ctcaatgaaa atcaaagagc
     8641 aaactaggag gctaaccgca ggttgctcaa aacactgttt tgaggttgtg gatagaactg
     8701 acgtggtttg aagagatttt cgaagagtct tacctcatca aatttgtaaa tatcatgagc
     8761 cttctctaga catcgtaacc aatatcaaaa aaagctaatt ctaaagcgac tgcttgattc
     8821 cagcgttgct gaagttctgt caaatcttct cgatttttac cgacacgatt gagttcgtca
     8881 accagaaatt gaacccactc tgcaaagaaa ggacctctgt ggagattgat ccattccgaa
     8941 tgaatataga cttcaggtaa agccaaatct ttagaacccc agtctaaata gagaccttct
     9001 gcaatgacca gcatgaccaa aagatgggca tagtctgatg aagccaccgc cgaatacatt
     9061 agatcctgaa aggcttttgt tacagggtgc aaagtcactt ctagatagtc attctctgct
     9121 acttttaact ctttaaaagc cttttggaaa taaccatctt catctgcttc aagaaagcct
     9181 agttgcttgg caaaacgaag cttggattca agtttatctg cgtgagctac gcaggcaccc
     9241 agcatggata agaaggcatc aaagaagtga taatcttgaa tcagatagtc ctttaagacc
     9301 ttattctcaa ttgtccccgc aaaaagttcc ttaacaaaac gatgattgat tgcagcctgc
     9361 caatccttct gactgctttt taataattct ccaacagtca aacctggctg aaatgcatag
     9421 tcttgtgttt ccatatttac ttctcctctc tttacttgtt agtaattaat aaaacaccaa
     9481 gaaatatcaa gcaaaatcgt aattccactt gatcctttta aagcacatcg agagcatttg
     9541 cagagagcta actaaacaag cctatccagt ttatataaac aaaaaactcc aattacaatc
     9601 aagaattaga gttgacttac aagattagat cgttcatttc gccatacgaa aaaacgtttc
     9661 acatttccct tcgccagtct taactgtatc aggttcaatg ggtatcatct cagcttaaag
     9721 caccccaaat gtctctatta tttaattact gaaccagtat agcaaaaaat gaaagcccta
     9781 gcaagatatt tgaccgaaaa atatctt
//
LOCUS       DAVKGU010000076         9704 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKGU010000076 DAVKGU010000000
VERSION     DAVKGU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.076"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            3..1253
                     /locus_tag="VW077_001656"
     CDS             3..1253
                     /locus_tag="VW077_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9455645.1"
                     /translation="MKTPQKLDFFCLTFGVSTLWLLNHYHYQDSCKNAEGGLILSRYA
                     GPGSHRYPVGFSGDTIISWNSLRFQPYFTATASNIGYSWWSHDIGGHMLGDYDEELQT
                     RWLQFGVFSPITRLHSSRSPFNSKEPWFFSETTSKIMKKYLRLRHQMIPYLYTMNVKT
                     HEEGAPLISPIYYFYPENDESYNVPNQYFFGTELMVAPIVEKMDLTFQSAKVDVWFPE
                     GEWYDFFSDKKYTGGVKLSVYRDISTTPVFAKSGAIIPLVGSEIGMGVDLPEVVDWYV
                     FPGKQHSFEMLEDQNGQRYKTRLSIDWEMGMVELALQGDSSIVPSNRKHRIHFKGTNV
                     SIIELPNKNDTAKFEWKDNKRTSLNDEVFRLLKTASLPYELKDRLLNQFINAKNSHDL
                     MNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(1475..1951)
                     /locus_tag="VW077_001657"
     CDS             complement(1475..1951)
                     /locus_tag="VW077_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9455646.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            2174..5377
                     /locus_tag="VW077_001658"
     CDS             2174..5377
                     /locus_tag="VW077_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9455647.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            5757..6133
                     /gene="tnpA"
                     /locus_tag="VW077_001659"
                     /pseudo
     CDS             5757..6133
                     /gene="tnpA"
                     /locus_tag="VW077_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6521..7150)
                     /locus_tag="VW077_001660"
     CDS             complement(6521..7150)
                     /locus_tag="VW077_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9455648.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(7160..8161)
                     /locus_tag="VW077_001661"
     CDS             complement(7160..8161)
                     /locus_tag="VW077_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9455649.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(8192..8833)
                     /locus_tag="VW077_001662"
     CDS             complement(8192..8833)
                     /locus_tag="VW077_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9455650.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(8852..9667)
                     /locus_tag="VW077_001663"
     CDS             complement(8852..9667)
                     /locus_tag="VW077_001663"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9455651.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         3014 a         1767 c         2099 g         2824 t
ORIGIN      
        1 taataaaaac accccaaaag ttagattttt tctgtctaac ttttggggtc agtacacttt
       61 ggcttttaaa ccattatcac tatcaagata gttgtaaaaa tgcagaaggt ggtttgattt
      121 tatcaagata tgcaggtcct ggtagccacc gctaccctgt tggtttttca ggggacacta
      181 ttattagttg gaattcctta agatttcaac cttattttac agcgacagca tctaatatcg
      241 gttatagttg gtggagccat gatatcggtg ggcacatgct gggggattat gacgaagagc
      301 tacaaactag atggctacag tttggtgttt ttagtccgat tactcgatta catagttcta
      361 gaagtccttt taatagtaaa gaaccttggt ttttttcaga aacaacatct aagattatga
      421 agaaatacct tcgtttgaga catcagatga ttccctatct atataccatg aatgtaaaga
      481 cacatgagga aggtgcccca ttaatcagtc caatctatta tttctatcca gagaatgatg
      541 agagctataa tgttccaaac caatactttt ttggaacaga actgatggtg gctcccattg
      601 tagaaaagat ggatttgaca ttccaatctg caaaagtaga tgtatggttc cctgaaggtg
      661 aatggtatga ctttttttca gataaaaaat acacaggtgg tgtgaagtta agtgtttata
      721 gggacatctc gactacgcct gtgtttgcaa aaagtggtgc aatcattccc ttggttggtt
      781 ctgagatagg tatgggtgtt gatttacctg aagttgtaga ttggtatgta ttcccaggaa
      841 aacaacattc ttttgaaatg cttgaagatc aaaatggtca aagatataaa acaagattat
      901 caatcgattg ggaaatggga atggtagagt tagcattaca aggagattct agtatcgttc
      961 caagcaatag aaaacataga attcatttta aaggaacgaa tgtgtctata attgaattgc
     1021 caaataagaa tgatacagct aaatttgaat ggaaagataa taaaaggaca tctttaaatg
     1081 acgaagtttt tagactacta aagacagctt ctcttccata tgaattaaaa gatagattgt
     1141 taaatcaatt catcaatgcg aaaaactctc atgacttaat gaatatcttg catcatcagg
     1201 ataaggaatt gagagggcgt ttgttggaaa tgatatttac tagccaaaac taattgatat
     1261 aattagaaag tcatttgttt agtgtatatg aagattctgt atggtattcg ttatcactgg
     1321 tcacattgaa aatgtgaaat aagcacctac aacatagact ttacaacact tcatgtttca
     1381 tatcatgggg tgtttttcta tttaagtcaa agccaatcaa aaaatctcac tattcatcag
     1441 gttaaaggaa acctaatcat agtgagattg tgaattatag tagagtttgt atcgcctctt
     1501 caatttgttt tgggtctgtt tttgaagaaa agcgttcaaa gacttgccca tctcgaccga
     1561 tgagaaactt agcgaaattc cattcgactc gttttcctag tgggccggat ttctggtctt
     1621 ttaaccagac atagagaggg tctgcttcct taccgttgac cttaatcttg gcaaaacgtg
     1681 ggaaggtggt ttgaaaatgt aggctacaga aggcgttgat ttcctctgcg ctgccgggtg
     1741 cttgtcccat aaactgattg caagggaaat ccaatatttc gaagccctga tcttgatagc
     1801 gttcatagag ttcttgaagt ccctggtact ggggcgttaa accacatcca gtagcagtgt
     1861 tgacaatcaa gagaactttg ccacgatagc tatccaaggg agttgcttgg ttgttttggt
     1921 tcaaaacgga aaaatcataa agtgaagtca tgagggcctc ttttcttttt ctacattgta
     1981 acagttttta cttttttcgt cgagtcggat ggaagatatt gttgaagaat aagccctcaa
     2041 tatgatagaa accgtttaca aataacttta aaaatggtat gatttaatta aaccggttta
     2101 ctttgttgct ataaatctct aatttgagat ttagttccta ttgattttac aatatgttta
     2161 ttggagtgta tacatgcaaa caaaaacaaa gaagctcatt gtgagtttgt cttcacttgt
     2221 tttatcagga tttttattaa accattatat gacagttgga gcggaagaaa cgactacgaa
     2281 taccattcag caaagccaga aggaagttca gtatcagcaa agggatacaa aaaatttagt
     2341 tgaaaatggt gattttggtc agacggagga cggaagcagt ccgtggacag gaagcaaagc
     2401 tcaggggtgg tcagcttggg tagaccagaa gaatagttct gcagatgcct caactcgagt
     2461 cattgaggct aaggatgggg ctatcactat ctcaagccct gagaaattaa gggcagcggt
     2521 tcaccgtatg gttcctattg aagctaagaa aaagtataaa ctgcgtttca agattaaaac
     2581 agataataaa gtcgggattg ccaaggttcg tatcattgag gaaagtggta aggacaagcg
     2641 attgtggaat tctgcaacga cgtcaggaac aaaggactgg cagaccattg aagcagacta
     2701 tagcccgact ttagatgttg ataaaatcaa gctggagtta ttctatgaaa caggaactgg
     2761 gactgtttcc tttaaggata ttgagctggt agaggtagca gcccagcttt ctgaggattc
     2821 tcaaacagat aaacagcttg aggaaaagat tgatttacca attggaaaaa aacatgtttt
     2881 ttctcttgcg gactatactt ataaggtaga aaatcctgac gttgcttcag tcaaaaatgg
     2941 aattttagaa cctcttaagg aagggacaac caatgtcatt gtcagtaaag atggcaagga
     3001 agtgaaaaag attcctttga agattctagc ctctgttaag gatgcataca cagcccgttt
     3061 ggatgactgg aatggcatca tcgctgggaa tcaatactat gattctaaaa atgaacagat
     3121 ggccaaatta aaccaggaat tggaaggaaa ggtagctgat agcctatcta gtatttcaag
     3181 tcaggcggac cgcacctatt tgtgggaaaa attttcaaat tataaaatgt ctgcaaatct
     3241 gactgccact tatcggaaat tggaggagat ggccaagcaa gtgaccaatc cttcttctcg
     3301 ttattatcaa gatgaaactg tcgttcgaac agtcagggat tccatggaat ggatgcataa
     3361 acatgtctac aatcgtgaaa agagcattgt tgggaactgg tgggattatg aaatcggtac
     3421 acctcgtgcc atcaacaata ccttgtctct gatgaaagaa tacttctctg atgaggaaat
     3481 taaaaaatat acagatgtga ttgaaaaatt tgtaccagat cccgaacatt tccgaaagac
     3541 gactgataac ccatttaagg ctctaggtgg aaacttagtt gatatgggaa gagtaaaagt
     3601 aatagctggt ttactgcgta aggatgatca agaaatttct tctaccattc gctcgattga
     3661 gcaagtgttc aagttggtag accaaggtga aggtttttat caagatggat cctatatcga
     3721 ccacaccaat gttgcctata cgggtgctta tgggaatgtt ttgattgatg gcctgtctca
     3781 actgttgcca gtcattcaaa agaccaagaa tccaatcgat aaagataaaa tgcaaaccat
     3841 gtaccactgg attgataaat cgtttgctcc tttgctggtg aatggagagc tgatggatat
     3901 gagtcgtgga cgctcgatca gtcgtgcaaa tagcgagggg cacgtggccg cagtagaagt
     3961 actaagaggg attcaccgaa tagcggatat gtctgaagga gaaaccaaac aacgtttgca
     4021 gagtcttgtg aagaccattg ttcaatcgga tagttattat gatgtcttta agaatttgaa
     4081 gacttataag gatatcagtt tgatgcaatc cttgttaagt gatgcaggag tcgcaagtgt
     4141 tccaagaaca agttacctat ctgcctttaa caagatggat aaaacagcca tgtacaatgc
     4201 agagaaaggg tttggatttg gcttgtcact cttttccagt cgtaccttga attacgaaca
     4261 catgaacaag gaaaataaac gtggttggta tacgagtgat gggatgttct atctttacaa
     4321 tggcgatttg agtcactata gcgatggcta ctggccaaca gttaatccat ataagatgcc
     4381 tggtacaaca gagacggatg ctaagagagc ggatagcgat acaggtaaag ttttaccgtc
     4441 tgctttcgtt ggaacgagca aactagatga tgccaatgcg acagcaacca tggatttcac
     4501 caactggaat caaacattga ctgctcataa gagctggttt atgctaaagg ataagattgc
     4561 ctttttagga agcaatatcc aaaacacttc aacagatact gctgcaacta caatcgacca
     4621 gagaaaactg gaatcaagta atccatataa agtctatgtc aatgataaag aagcctccct
     4681 tacagaacaa gaaaaggatt atcctgaaac ccaaagtgtc tttttagaat cgtccgattc
     4741 gaaaaagaat attggttact ttttctttaa gaagagttca atcagcatga gtaaggcttt
     4801 gcaaaaggga gcctggaagg atatcaatga aggacagtca gacaaggaag ttgaaaatga
     4861 atttcttacg attagtcagg ctcataagca aaatggagat tcttatggct atatgctcat
     4921 tcctaacgtg gatcgtgcca ccttcaatca aatgataaaa gagttagaaa gtagcctcat
     4981 cgaaaataac gaaacccttc agtctgttta tgatgccaaa caaggagttt ggggcattgt
     5041 gaaatatgat gattctgtct ctactatttc caaccaattc caagttttga aacgtggagt
     5101 ctataccatt cgaaaagaag gggatgaata taagattgcc tactataatc ctgaaaccca
     5161 ggaatcagct ccagatcagg aagtctttaa aaagctagag caagcagctc agccacaagt
     5221 acagaattca aaagaaaagg aaaaatctga agaggaaaag aaccattcgg atcaaaagaa
     5281 tctccctcag acaggagaag gtcagtcaat cttggcaagt ctagggttct tgctacttgg
     5341 ggcgttttat ctattccgta gaggaaagaa caactaattt gttcatcatc tagatgaatt
     5401 acatgaaact gtggaaaata gaaaaaatcc gaagaaggcc aaatatcttc ggatttttct
     5461 tttaaaaaag gataagttga tacttatcct ttttacttca ttatgtgact atagcgtttt
     5521 gaaactagaa taggacatca taactgctaa aagatttcta taaattcatt tgattttcct
     5581 aatcaatttg ttcgtatcct atttcactcc actatattag tccgtctcgc tccgtgaggt
     5641 gcgagacaaa acaaccaccc gctatacggg tgcgcgtcga aggttatacc aaaaaaactc
     5701 caaacgcgat acaataaagg tgttcaatcc acttgtaaag cgaaaggaga aaaaatatgg
     5761 cacaaaaggc tcatagttta tctcacacaa agtggaggtg ttctatcaca ttgtgttcac
     5821 ccctaagtat agacgaaaag tcatctataa tcaatataga agtagtttag gcgaaatatt
     5881 tcatcgcttg tgtagttata aaggtgttga aatgatcgag ggtcacttaa tgccagacca
     5941 tgtacacatg ttagtcagta ttccgtcgag gctaagtgtt tcaagtttta tggggtattt
     6001 aaaaggcaaa agtgcactca tgatgtttga caaacacgcc aatctcaagt acaagtttgg
     6061 gaatcggcat ttctgggcgg aaggttatta tgtaagtcca gtagggctta ataaagccac
     6121 aattaagaaa tatagtcaag attaaagaaa ttatccagtg gatgatttct tcacgagtat
     6181 gaaaatttga gaacgagtaa agcatgatat atagtaaaat gaaataagaa cagaacaaat
     6241 cgatcaggac agtcaaatcg atttttaaca atgttttaga agtagatgtg tactattcca
     6301 gtttcaatct actatagcac tagataaatt gagtgtaaaa gaatatgagg atccctttag
     6361 ggatagtggt aagtaatacc aaagtctctt aaagaggcaa gtgacgagtc aagagcaata
     6421 aggcttgaac aacgtgaaag ccagcgtctt taggcgcttg gctgatgatt tgggcttata
     6481 gctctgggat aaaccacccg tttgacgggt ggttatgatt ttatctgagt gtaacatact
     6541 gttgggcaat ctcgctgatg cggtcaaagt tgccttggga agcgagttta ttgagttcgc
     6601 caccaattcc aacggcatct gcaccagcag cgaaccattg agggatgttg tttaggccga
     6661 ctcctccggt taccattacg gaaacttgtg ggatcggtgc cttgactgca gagatatatg
     6721 ctggactgag agtactacct gggaagagtt tgatgatttc actaccggct tcaagtgcag
     6781 tcgtgatctc tgtgagggta atacagcctg gaatgtacgg tgtgctgtag agattgcaca
     6841 ttttcgcagt ttcagcatgg aaagatggag aaacaacgta atttgctcca gctagaatgg
     6901 catctctagc agttacggca tcaagcacag tacctgcacc gatacaaaca ctctgatcgt
     6961 cctgatacaa gtctacaagt tccttgatga tttgtcctgc atactgattg gtataggcga
     7021 tttcaatagc tttgataccg cccttgatac aagcaatcga ggcttgtagt ccttcttcct
     7081 ttgtatttcc tcgaatgaca gcgacaattt tctgtttttt tagttcaata atcgtatctg
     7141 atttggtcat gtaattctcc taacgaatga tatcttgtgc atttgccagt aaattttcaa
     7201 tactagttgc ggaagtggag agatggtctc ctggaagagt gcatttgaga gttgcgctcg
     7261 caactgcaaa gtcaatggta gtttttaggg aggaatgatg gagtagttgg taaagggcac
     7321 cagatataaa ggcatccccg ctaccaattc gttgatagac tgcagttttt agttggacag
     7381 actcttcaaa tcgttcttcc agagcatagg cttgatagac atttttgtct tgctcatcac
     7441 tagatcggag ggtatggaaa atggccttga aaccataggc ttcttttaaa agtcgcatgc
     7501 gattttccac ctcttctagg ctagcactgt ctcttggaaa catctctaga ttttggtcat
     7561 caatcatgag aggatcaata ccgaagcaat agtcagtaaa acgtgcaaac ttagaaaatt
     7621 catacttggc ttctaggact gaaatcatct ttgttctcag attgagatcc attgaaacga
     7681 caattcctcg gcgcttggct tcttccaaga gtaggagaag gatcgcacgg acctcttgac
     7741 cgatagctat ggtgattcca ctaaaatgaa aatggctaat cccctgaaag agagaatcca
     7801 tatctagcat gtttggccga atctggctga tactcgtatg cttacgatcg tagaaaactt
     7861 cactttgacg acaaccaaag ccgttctcca aatagtagag gccgattcga tcgccaagcc
     7921 gacagattga actggtatcg atttggtgct gtttcaagaa tgtgagaaaa cgatctccaa
     7981 tctcgttggc agggagtgcg gtaaagactt tcgttgagat acccagggct tgcaaattac
     8041 aagcgatgtt aatttctgat ccgccaaaat aagtcgaact ggcaacatga tcgccgatac
     8101 tggtggcatc taatggtgag attcgaatta atggttcgcc aaacagtaat attttcgtca
     8161 tccattagtt cctttctggt ttagaatagc tttacttggc taatacttct ttcaaataag
     8221 cagcgacttc atcatcttgg cagttttcaa agaagtattc ttggaagtat ttgccagaga
     8281 tggtgtcttt gaggaagtct tggtcgattg ttttaaggat ggtcatcaaa tcattgtggg
     8341 tgatttgttt cacctcgttt aagatacgag cgttgcgttg ttcagggatt acacgttctc
     8401 ttgggtagcc accgcccatt tcttcagcaa ataagcgttc aaacatcaat ttgagggtca
     8461 gttctgcccc ccagccaaat cctttggcat aagggataga caaggcgtta ccaccattga
     8521 tttgagaata aaggtaagcg tcagttgggt ccactgctag accacagaca acaccaggga
     8581 agctgtttaa agcaagcata gcccctacac ccgtaccaca gccggtaaca acaaagtcaa
     8641 ctgcctttgt atttaaaagg atggcagcca ttagtccgtt ctgcacataa gttaattgac
     8701 tttctccttc ttctccacgc ataccatagt taaataattg gtagcctttt ttatctgtcg
     8761 cttcttttag actatcgtaa ataatgtgat tcttgctagc ttgactattt tcattgatta
     8821 atgcgatttt cataagatct tctttctatt tttactcagg ttgttttccg atgtaggcta
     8881 agataccgcc atctacatat aggatgtggc cattgacaaa attgctggca tcactagcga
     8941 gaaagacagc agggcccatc aaatcttcag gatttcccca acgtgcagca ggtgtttttg
     9001 caatgatgaa ctggtcaaat gggtggcgag aaccatcttc ttgcaactca cgaagaggtg
     9061 ctgtttgagg agtggcaata taacccggtc caattccgtt acattggata ttggctccac
     9121 cgtattcaga cgcaatgttg cgggtcaaca ttttcaagcc ccctttagca gcagcataag
     9181 cgctaactgt ttcacgtccc agttcgctca tcatcgaaca aatattgata atctttccat
     9241 gccctttctt tatcatagaa ggaataactg cctttgaaac gataaatggt gcgtttaagt
     9301 caatatcgat gaccttacgg aaatcagcgg cgctcatttc gcacattgga actcggcgga
     9361 taataccagc gttattaacg aggatgtcaa tgacaccaac ctcttgttcg atttgcttga
     9421 ccatggcttg gataccgtcc tcgtctgtca cgtcacagac atatccatgg gcttcgatgc
     9481 caacttcacg ataagcttca atccctttat tgaccagatc ttgattgata tcgttaaaga
     9541 caatagtagc gccggcttca gcgtaggatt tggcaatagc aaatccaatt ccataagaag
     9601 cgccggtgat gagagcaatt tttcctttta aagaaaactg ctcaattgag aatgatgtat
     9661 ttgtcatttg atacttcctt tcaaatttgt taagacttct ctat
//
LOCUS       DAVKGU010000077         9419 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKGU010000077 DAVKGU010000000
VERSION     DAVKGU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.078"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(93..485)
                     /locus_tag="VW077_001664"
     CDS             complement(93..485)
                     /locus_tag="VW077_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9455652.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNSFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNITEKLDKLVLEND"
     gene            complement(784..1413)
                     /locus_tag="VW077_001665"
     CDS             complement(784..1413)
                     /locus_tag="VW077_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9455653.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(1458..1697)
                     /locus_tag="VW077_001666"
     CDS             complement(1458..1697)
                     /locus_tag="VW077_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455654.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(1847..2113)
                     /locus_tag="VW077_001667"
                     /pseudo
     CDS             complement(1847..2113)
                     /locus_tag="VW077_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2138..2449)
                     /locus_tag="VW077_001668"
     CDS             complement(2138..2449)
                     /locus_tag="VW077_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9455655.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(2374..2616)
                     /locus_tag="VW077_001669"
                     /pseudo
     CDS             complement(2374..2616)
                     /locus_tag="VW077_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2617..3249)
                     /locus_tag="VW077_001670"
     CDS             complement(2617..3249)
                     /locus_tag="VW077_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9455656.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQDEF"
     gene            complement(3263..4258)
                     /locus_tag="VW077_001671"
     CDS             complement(3263..4258)
                     /locus_tag="VW077_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9455657.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHNLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4255..4953)
                     /locus_tag="VW077_001672"
     CDS             complement(4255..4953)
                     /locus_tag="VW077_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9455658.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5030..6040)
                     /locus_tag="VW077_001673"
     CDS             complement(5030..6040)
                     /locus_tag="VW077_001673"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9455659.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(6024..7031)
                     /locus_tag="VW077_001674"
     CDS             complement(6024..7031)
                     /locus_tag="VW077_001674"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9455660.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7018..7971)
                     /gene="mvaD"
                     /locus_tag="VW077_001675"
     CDS             complement(7018..7971)
                     /gene="mvaD"
                     /locus_tag="VW077_001675"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9455661.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(7953..8831)
                     /gene="mvk"
                     /locus_tag="VW077_001676"
     CDS             complement(7953..8831)
                     /gene="mvk"
                     /locus_tag="VW077_001676"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9455662.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8953..9282)
                     /locus_tag="VW077_001677"
     CDS             complement(8953..9282)
                     /locus_tag="VW077_001677"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9455663.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWAQ"
BASE COUNT         2788 a         2174 c         1642 g         2815 t
ORIGIN      
        1 ctaatgcagc caattataga caacaagcct ttttcctaac taaaacactc ataaagtata
       61 gacaatttta gactactttg gatatctttt ggttagtcat tttccaaaac taatttatct
      121 aatttttcag ttatatttga cttcatcttc tcagtaacat gagtataaac acttaaaatc
      181 tttgtttcat tagtataatc tacacgatcc attatcgctg ttataggtac ttccaattca
      241 gcaagcaaac ttatatgcga atatctaaaa acatgagcag tcaatacaaa atcatctttc
      301 ttaatttcac ttttcctaaa actgtttttc aaaaagtaat tgaaacaagt tatcattaag
      361 ggagaaccat tatttgtaaa tatataatca ccttcttcta aaagaagata ttcttttaaa
      421 atttctaaca ctctatcact aataccaatt actctatatg aagctaaact tttcggacta
      481 tccaaaaata cttcgttatt ggacgattac attatagtaa aacgaaataa gaacaggaca
      541 aatcgatcag gacagtcaaa tcaatttcta acaatgtttt agaagtagag gtgtactatt
      601 atagtttcaa tctactatac aagacaaaat tgaagaaatc tttccattat aaaacgcata
      661 ttatcaaggt ttttaaacac ctaataatat gcgttttttc ctattttaaa gacttttatc
      721 cacctattat acaaagttaa gtatccatca cagtttacaa ttagccttca ttacctaacc
      781 cattcaccat ccttcaagag agtaaccttt aagaaccgaa taaataaatg gcaaatttcg
      841 ttcatttaat ttaactgcac tgttaatttg attagctcca tctcctaaag tatgcactcc
      901 tactacacgg tgactagcat cataaactgt agatccacta gacccctcta aagtatcaac
      961 ttggtaatcc aagaacatac catcatcact taaaacttgt tttttatctg tatacatttg
     1021 atgaatttta aaattatatg atagatagcc tgtgatagtc acagttattc ctgtcaaatt
     1081 tttttgacta gtaggaagac ccaaagtccc taattttgca ccaatgggct cttctagaat
     1141 taataaagcc aagtcatatt cccttgcatc cttagaattt aaatttctaa attccttcaa
     1201 ataacgaact tcctttactt tgatctttcc aaatagttct tgacttggac taacagccgg
     1261 aagaacataa atatcatccg cttctttccc atagtcatgt ctgtagtagt tgtgagccgc
     1321 tgtaattaac acattatctt taataaaatt cgcacttccc ctcccttcta cattcattcc
     1381 atcggctact ccataatact tagataaaac catagctgaa gtcgaatacg gatactgtaa
     1441 agtattatca attttaatca aatcatcatt accgataata cttctgattg cttttggtag
     1501 tatgaaccat acgttggtga aatctcagat aatgaagaat cattagactc tggacctttt
     1561 tctagtgtct cacttacctc atattcttca cctgtactag aaataacact caaagcagat
     1621 actgtcgata actggctagc caataaagta ctcgcaataa ttgaaatacc caatttttta
     1681 taaacagttt tcttcattat tgtgtcctcc taatgtaatt atagcgtact attctaaatt
     1741 tcttaatcta ctatagaatc aagaaatcta ccaccttctt taaataccct ccattatcac
     1801 ataaacaggt aaacttttca attaatgact gcgcttttca atcacgctag aggtacttac
     1861 ttgcttcttt gatactaagt tcagccattc tttccctgtt tttctcaata aagcatgtta
     1921 cccaagtggg attcgttttg gagtagtctc gcagagtcca gccaatggct ttattgataa
     1981 aaaattctgt ttggttcaag ttatgaagga gaatcttttc cattaattga gtattggtct
     2041 tctcttttct taacaactgg tggtcaatag cgacacgtct cagccagata ttatctgata
     2101 ggctccattt tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcagattgc
     2161 tcaaaacact gttttgaggt tgcagataga gctgacgtgg tttgaagaga ttttcgaaga
     2221 gtattaagat tatttcttct agttcagggt gttcatacac caaactccct actactcgat
     2281 ctaggatatc taccgtgtcc caccaggatt ttgtcacgac taactgctct agcttaggca
     2341 aatcggtttc ctttagataa gactgcattg ctttcaaata gttagcagcc acatattggt
     2401 attttctagg ctccttttcc cagcaagtgt ctgcaaaatc ccaatcgata atctttgttt
     2461 ttttcgcttc tagtaaatat ttttatagag tttatttctt tcaggcaccg caatacctag
     2521 aaaagaaaat tgatggcgca tataggcttc catggacctt gcttttttag agtcttttgc
     2581 tgcttctagc tcctcaagta aatctgctaa actcatctaa aactcatctt gccccaccaa
     2641 atggtgctga aaggcataga cagccgcctg ggtacgatcg ctgacttcaa gtttggcaag
     2701 aatgttggac acgtgggtct tgaccgtctt gagagagata aagaggtcat ctgcgatgcg
     2761 ctgattttcg tagcccttgg cgatgagttg gagaacatct cgctcacgcg cagtcaattc
     2821 ttcatgaagt tccatatgat tgcggtggta ttcaaccttc ttgctaacct cttgctcaat
     2881 ggccagctcg ccagcagcta ccttactgac ggcatgaagc aattcatctg cactagaagt
     2941 cttgagcata tagcctttgg caccagcatc taagactggc atgatttttt cattgtccaa
     3001 ataagaggtc acaatcaaaa tcttggcttc aggccattct ttaaggattg ctaaggtcgc
     3061 gtcaattcca ttcatctcag gcatgacaat atccatgaca atgacatctg gacgcagttc
     3121 caaggccaag tcaatccctt gagacccgtt ggacgcctca cccacaactt ctacatcgtc
     3181 ttggaggtca aagtagcttt tcaagcccaa acggaccatt tcatggtcat ctactagtaa
     3241 aattttcatc tttactcctt tatcattcct tatctaacag gggaatacgg atatcaactg
     3301 ccagcccttg cttgggagct gttaataact gaaccgtccc tgccatatct tcaacccgct
     3361 ccttgatatt tcgcagtcca taactcaagt cgtctaagct ccctaactgg aaaccaatcc
     3421 cattgtccac caccttcagt tgcaattcaa catctgtctg atagaggtag acatctaggc
     3481 aagatgcctg ggcatggcgg agcgtattgc taatcaactc ttgcaggata cggaagatat
     3541 gctcctcgat tttcttaggc aatttcgtca tattctgctt gagactaacc ctaagatcac
     3601 tcttgtcctc aagctctttt aagagaattt gaatcccttc tatcaagctc ttctgctcca
     3661 gttcaactgg tcgcaaatgc aagagcaaaa cccgcaaatc cttctgggct gtttctaaaa
     3721 tagctgtgac actctgcaac tgggtctgca tcttttctct atccaatttc aaagcctgct
     3781 gactgatacc cgataaaatc atgtgggccg caaacaactc ctgactgact gtatcgtgca
     3841 aatcccgagc aattcgcttc cgttccttct cgatgatttc ctcttcctga gcaaggctat
     3901 gattttcagc tttttgaaga gcttctgtca aaaggttaag tttacctgat aaggacttga
     3961 aactggcatc caaatctgga tctgcaacct gaaccacttc ttgccctgcc aataaacgct
     4021 tgagattagc ctgcattttt cttagagaaa gctcttcgat ccctcgccaa aacagggcta
     4081 agagacaggt tatggacatg ctgaaaacca acaataaaaa gacaaatttt tctgtttttt
     4141 cgacatcgtg caaaaagata gaccagtcaa aatcaagtat ttccagcaag ttgtgggaga
     4201 aaaaaaagac aaacaggaag gaggtgagag caataatgac ataggcttgt tttttcatcc
     4261 tctaaccacc tccacatcac caatcatagt ggtcaagaaa atcttgacac tcttgttact
     4321 cttgagatag tcttttgttt cttgatgata gtgttcattg cggagggctc gcttgggctg
     4381 gttgaaaaaa gtcaaatccc catagagaca gttaacgctg agactgactt ccacatctac
     4441 aggtacgatg attttggtcg ttcctaccat ctttctgagg ataatgacat tgtcatgatt
     4501 ggttaagatg accctctcca gatgaatagt gtccttgccc atgaagcgaa agagattgat
     4561 atcatcgaat tggcaagtct ggtagcttga aaaatgatga agatttccaa accaacgatt
     4621 tttctccttc ttaaccgtca cgacctcttc aaaaaccaaa ttggtctgct ctttttcctg
     4681 gttcatcatc gggtaaagaa gaaagaggct atagataacc gcaacaaaaa tagctagaat
     4741 cacaaaagga ttgagcataa cgatgaaaaa gaagagaatg gttgccgcta ctaaaagaag
     4801 attatttccc tctttaccag tgtagtagcg aatcaaaagc aaaaagagga atagtatcag
     4861 cagaaaacgc gaaaaatgct ctgataccat caaaatcaga gctcctgtca gaagacaggc
     4921 ttcgataaat aaaaagattt taaattttct cataggttca tcctctccct tctattttat
     4981 cacaattcaa aaaagtcacc tcagtctgag gatggaaaaa aggcgctggt tacgcctttt
     5041 tcatctgatc ctttgcttct tttaattttc cataaagaag atagtctact ttttgtagat
     5101 ctgctatggt ggcacagtta agggaacaca taatcaagcg taggtctgct ttccagcctt
     5161 ggacaatgcc aatcacttct tcaactgtgt aggtttcaac caattccaga acggttcgtg
     5221 acaatcccac agacttagca ccaaaaacca agcacttaat catatccagc ggattccgaa
     5281 cccctccact aaccaagagt tcgaccttat ctttccattc ttgggcattg agaagggcct
     5341 gcatggtaga ctgaccccat tgattgaggt aatcacgctg gccactacga cggttttcga
     5401 tataggcaaa gctggtgcca ccacgacccg ataggtccac tgtacgaaca ccgaattcat
     5461 aggctctttc gattgtcttg gcatccattc caaagcccac ttccttgagg acaataggaa
     5521 cgggaatttg ctcgctataa tctgctagat gcgattgcca gcttctaaac ttcctttctc
     5581 cctcgggcat gagtaattcc tgcatgacat tgacatgcac ttgcaataga acaggattca
     5641 tctcttctac agtctgaagt cctaactcga caggcttgtc caatccaata ttggttccaa
     5701 ggaggagatt gggatgacta gacttgacag aaaaagaatc atccgttgga tttttgaggg
     5761 ctgcgctata agaacccgtt acaaataaaa taccacagga ttccgccacc tgagccagct
     5821 tttgattgat ttctcttccc ttattacttc caccagtcat ggcattgata taaaaaggaa
     5881 agtcccactt tcgaccagca aactctgtcg aaagatcgat ttcatccaga ttgtaaagag
     5941 gcaaggaaga atgaatcagc tccacctcat caaagctatt ataggaactt ttctgctcaa
     6001 gggcatagag gatatgctca tccttacgat ttgtcgtcat gtcctatcct ttcttgatat
     6061 aagagctcaa tccccagatc ggcccaacga ttttttaagg tttcggttga ttgcgcatca
     6121 aaactcaagg cgatgccaca gtcaccacca ccagcaccac tactcttggc aacggcctgc
     6181 aaatcttgac tggcttcttt caactgtcta agcaaaggcg tgtaaatatc tgtactcaag
     6241 ccttctaaaa gcttgctggc tacttctact tgctcgataa ctttttcggc tttcccctgc
     6301 tccaaggctt cgaccaaaga aaccaccgtt tcttttgagg aacttaaaaa attttgattg
     6361 atattttgct tgatttgctg gaccatgtga ctcgatacag ccacttcctt ggtccatccc
     6421 actaagaaat cacattctaa agttggtttc acttgtgaga taaaaaatcc ccaatcacgc
     6481 tccagaactg tcgccaagtt ttcttcttct aaccaagcag ccaccttctg gcgatcaaat
     6541 gactggtaga gaaccaaatc ctctgccaca atacaggcaa ggtcgcccat ggaaccattg
     6601 tctcctcgct tgagcaagac agcgctagtc agcttgaaca agagattctg atcaaccgaa
     6661 agattataga gagccagtaa agccttgaca accaagacaa cgacgctgcc actagaacct
     6721 agaccaaact ttttcccttc tcgttccatt ttgccacaga tttttaggga aaaaggtctt
     6781 aaattctgac cgcgaacagc gaggaagtct cccatcaaag caatcgtttc ttgaatcaag
     6841 ctgtagtcag gattaggcct taagtccact gcgaaatcaa acatatctga atagatacgg
     6901 tagctgtcag aaaaagcaat ttcagccctc atatagatgg gaatatcctt tatcaacgct
     6961 aactgccctg gctctaaaat agcatattca cctgcccaat agagttttcc gcaagtttta
     7021 acagcaatca tcttgactca aatcctttgt ttttgacaca atcaagcgat aacgctgacc
     7081 gaaaatttct gataaatgct ccaagtcttt ctcctgacag aagaccttaa cattgggacc
     7141 agcatccatg gtaaagtagc aggcctctcc tttctcacga agctggcgaa caaagtccat
     7201 agcctcataa gaggcatccg tcagataaga aaaggctgga ctagcagtct ttgtcgtagc
     7261 atgcatagcc agggcatttt tctccgttaa ttctccaatc ttggcaaaat cattttcctt
     7321 gagataaatc agcatatcct gatagtcctt ctcagactga cgaacccagt cgtcgaaagt
     7381 cgtcgaggtt tccacacaaa gtttcatccc gtcacggcta gagattggtt ttttcttgtc
     7441 ctctagcacc aacataatca tagctagttt caagtctgtc tctacagggt aaatttctcc
     7501 actatcctta tcccaggctc ctagtggtcc ataaaaactc cgagaagaag agcctgaggc
     7561 aaacttggct tcctgcgcta actgacttct atccaatcca agcttgaaat aagcattaca
     7621 agccttgacc agggcggaca aaccactaga acttgaggac aggcccgctg ccgtaggcat
     7681 attgttttga gtatcgatac ggacaaagcc ctcaccagct ggacgataac ggtcaataat
     7741 cttactcatc ttggcatgct cgacctcatt ttgtagctga ccattgatgt aaaattcgtc
     7801 agctgttaca ttggctggta aaggcgacaa ggtcgtctct gtatacatat tttccaaagt
     7861 tagagaaata ctgctagtag caggcaccat ctctttttct tttttctttc cccaatattt
     7921 gataatagca atatttgcgt aggaacgtac tgttacaggc tctctatcca tgtctgaaca
     7981 gctcctttct cttctaatct ttctgctagt tcttgtgcgt gtgtcaaatt ggttaccaag
     8041 gctatgatac aacctcctag cccaccaccg ctcatcttgg cacccagagc accatggcta
     8101 agagtcgttt caaccaaaaa gtctgcctca gggctactga ctccaatttc ttttaaatgt
     8161 aaatgcgctt gactgaggat ttgtcccagt ccttcagcat ctttttgtga aatcgcaact
     8221 tctgcttgct gggttaattc tcccaaggca tgcaaaaacg gtagggcatc cttgccctta
     8281 ttttgaacca cttggatggc ttcacgagta tgaccataaa cacccgtatc ggcaatcacc
     8341 aaataggcgg ataaatccat ctcaagttct gtaaatccta cgttcttgat aaagcgaata
     8401 ggttggtcac tgagacaggt cttagcatcc aaaccactag gattcatatg ggcaatcatt
     8461 tcagctcgat tgaccaagat ttctagtaca tcatgaggca gatcagcctg atagtagtca
     8521 aataccgcac gaatggccgc tatgctgata gccgctgacg aacccatccc ccgtttctca
     8581 gggatagccg agtcaatctc acaacgaatg caggcttctg tgatatccaa atactccagt
     8641 gaggcataaa ccgccatgga caaggtatcc tcctcataaa ggcgccaagg actctctgca
     8701 ggaactacct tacaggtcac ctccacctcc aaaagaggca gggaaatggc aggataaccg
     8761 taaacgaccg catgttcccc tattaaaatt atcttactat gtgcctgacc gacaccaact
     8821 ttttttgtca ttttttcctt ttactagacg aaaaaacgtc ttatttttca tacaagtatt
     8881 aattctttcc tatctatttt attatatttt cacaaaaaaa gcgattgttt ccattcacaa
     8941 tcgcttcttt cattattgag cccattcgcc attatagttg acagaatagc catctacggt
     9001 cgtattcact gccaaggcac ctgagctata agcgtagtac catctgccat tgacctggaa
     9061 ccaacctgtc gtcatagaac gacgaaagaa actccatacc attaagtaaa gaggaaagtc
     9121 gtgagggagc atgcgccatt gacaacctgt tttagtgacg tacaaagtct cattaacaag
     9181 tactcgtttc ggccatttat aggtgcggtg tttggagaaa tagggttcaa tcttcgccca
     9241 ttcttgatcg tttaaatcag tatcatatgc tttacgtatc ataactctag tttaacattt
     9301 ttttgtgaat acaggttcta aataatcgac cacgaaaatt tcttaagtgg aaaacgcctt
     9361 atgaagtatg ctacgggaaa cttatgcact taatttgaca attcaagatg taaaaatat
//
LOCUS       DAVKGU010000078         9243 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKGU010000078 DAVKGU010000000
VERSION     DAVKGU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.079"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..432
                     /locus_tag="VW077_001678"
     CDS             <1..432
                     /locus_tag="VW077_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9455664.1"
                     /translation="SRKLSDKRFYRPTFRMHLTNKEILNKLLSYSEDLKHHYQLYQLL
                     LFHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLHYSNAKLEATN
                     NLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(531..2153)
                     /gene="groL"
                     /locus_tag="VW077_001679"
     CDS             complement(531..2153)
                     /gene="groL"
                     /locus_tag="VW077_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9455665.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2169..2453)
                     /gene="groES"
                     /locus_tag="VW077_001680"
     CDS             complement(2169..2453)
                     /gene="groES"
                     /locus_tag="VW077_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9455666.1"
                     /translation="MLKPLGDRLVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2609..3004)
                     /locus_tag="VW077_001681"
     CDS             complement(2609..3004)
                     /locus_tag="VW077_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9455667.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EVDFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3082..3843)
                     /locus_tag="VW077_001682"
     CDS             complement(3082..3843)
                     /locus_tag="VW077_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9455668.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3885..4511)
                     /locus_tag="VW077_001683"
     CDS             complement(3885..4511)
                     /locus_tag="VW077_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9455669.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFETAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(4527..4844)
                     /locus_tag="VW077_001684"
     CDS             complement(4527..4844)
                     /locus_tag="VW077_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9455670.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(4841..5140)
                     /locus_tag="VW077_001685"
     CDS             complement(4841..5140)
                     /locus_tag="VW077_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9455671.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5425..5616)
                     /locus_tag="VW077_001686"
     CDS             complement(5425..5616)
                     /locus_tag="VW077_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9455672.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5777..6451)
                     /locus_tag="VW077_001687"
     CDS             complement(5777..6451)
                     /locus_tag="VW077_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455673.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            complement(6457..6903)
                     /locus_tag="VW077_001688"
     CDS             complement(6457..6903)
                     /locus_tag="VW077_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9455674.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7017..7520)
                     /locus_tag="VW077_001689"
     CDS             complement(7017..7520)
                     /locus_tag="VW077_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9455675.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7517..7879)
                     /locus_tag="VW077_001690"
     CDS             complement(7517..7879)
                     /locus_tag="VW077_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU9455676.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7945..8976)
                     /locus_tag="VW077_001691"
     CDS             complement(7945..8976)
                     /locus_tag="VW077_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9455677.1"
                     /translation="MGFLLMGALFIVLPRTMVSAKRINQVLDLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQKVAIVGPTGTGKTTIVNLLMKFYEIDKGSIHI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            complement(8983..>9243)
                     /locus_tag="VW077_001692"
     CDS             complement(8983..>9243)
                     /locus_tag="VW077_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455678.1"
                     /translation="TGIRVVRAYNAEDYQNEKFAAVNDELTRLNLFVNRLMAILNPIM
                     MGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIIFMSYAM"
BASE COUNT         2677 a         2175 c         1716 g         2675 t
ORIGIN      
        1 agccgtaaac tgagtgataa gcgattttat cgtcctactt ttcgtatgca cttaaccaat
       61 aaagagattt taaacaagct tttgagctat tcggaagact tgaaacacca ctatcagctc
      121 tatcagctct tgctttttca cttccagaac aaggaaccag agaaattttt cggacttatt
      181 gaggacaatc ttaagcaggt tcatcctctt tttcagactg tctttaaaac cttcctcaaa
      241 gataaagaaa agattgtcaa cgcccttcaa ctacactatt ctaatgccaa actggaagcg
      301 accaataatc tcatcaaact tatcaagcgc aatgcctttg gttttcggaa ctttgaaaac
      361 ttcaaaaaac ggatttttat cgccctgaat atcaaaaaag aaaggacgaa atttgtcctt
      421 tctcgagctt agctgacttc aacccactac agttgacaaa gagcctataa aaagaagggt
      481 tagtcattcc ctccttttgt gttttttata agttgttttc tatagaaagc ttacatcatc
      541 ccgcccatca tacttggatc cattgctgga gctggggcta ctggttctgg tttattggct
      601 acgactgctt ctgttgtcaa aatcaagctg gctacagatg ctgcattttg tagggctgaa
      661 cgactcactt taactggatc aatgatacct tgatcaatca tgttaaccca ctcgccagtt
      721 gctgcgttaa atcctatacc aagctcagca tttttcaaac gatcgataac gatagatcct
      781 tcaaatcctg cattgtgagc aatttgacga acgggttctt ccaaagcacg gagaacaata
      841 ttacgtcctg ttgcttcatc tcctgtcaat tccaaggtag caacagctgg aatcacattg
      901 gcaagagctg ttccaccgcc tgcaacaata ccttcttcaa cagctgcacg agtagcgttg
      961 agggcatctt caatgcggag tttcatttct ttcaactcag tttcagttgc ggctccgacc
     1021 ttaataaccg ctacaccacc tgacaatttg gccaagcgtt cttgcaattt ttcacggtca
     1081 aattcagaag ttgtagtttc gatttgagac ttgataaccg caacacggtg agaaatcgct
     1141 tcaggatttc ctgcaccttc tacaataacc gtgctatctt tgtccacggt cactctcgct
     1201 gcttgaccaa gagcttcaat tgtcgcatct ttcaactcaa gaccaaggtc ttctgtgata
     1261 actgttccgc ctgttaagat ggcgatatct tcaagcatgg ctttgcgacg gtcaccaaaa
     1321 ccaggtgcct tgactgctac tacgttgaag gttccacgaa tcttgttcaa aacaagagtt
     1381 ggaagagcct cgccatccac atcatccgca ataatcaaga gtggacgatt gctttggaga
     1441 atgctttcca aaagtggcaa gatctcttgg atattggaaa ttttcttgtc tgtaatcaaa
     1501 atgtacggat tttcaaggtc agccaccatt ttttcgctat ctgtcaccat gtactgtgaa
     1561 aggtaaccac ggtcaaactg cattccttct acgacttcaa gctctgtttc cataccacgt
     1621 gactcttcga tggtgatgac accgtctttg ccaacttttt ccattgcttc agagatgtac
     1681 tcaccaactt tttcagaacg agaagatacg gctgcaactt gagcgatagc ttctttattg
     1741 gcaacaggga tggcgttgtt tttcaaagct tcaactgctg cggcaactgc tgtttcaatc
     1801 ccacgacgaa taccgattgg atttgcacct gctgtgacgt ttttgattcc ttcacggacg
     1861 atagcttggg tcaagactgt tgcagtcgta gtcccgtcac ctgcgatatc attggtttta
     1921 gaagctactt ctgataccaa tttggcaccc atattttcaa aatggtcttc taattcaatt
     1981 tctttggcaa tagtcacacc gtcattggta atcaatggtg agccaaatga tttttcaaga
     2041 acgacattgc gaccttttgg tcccaaggtt actttaacag tatctgcaag gatatcgaca
     2101 ccacggacca tagctgaacg ggcatctgat gaaaatttaa tttcttttga catacttact
     2161 ttctccttct attcttcaat gattgccaag atgttagctt cgcctacgat gatatacttt
     2221 tcatcgccat ctttgacatc aagacctgcg tgggcttcaa ctaagacacg gtctccagtt
     2281 ttaacacttg gagcaaccaa gtcaccgttc aaggtacgaa caccttgtcc agtagccaca
     2341 acttgggctg ttttggtttt ttcttgggct gaacctgcga gaacaaagcc tccaacagtt
     2401 tgttcttttt cttctacttt taagaccaaa cggtccccta atggtttcaa catctgattt
     2461 cctccataat gagatagata ttattagcac tctttatacc tgagtgctaa ttcatagttc
     2521 tattgtatca cttagtcaga aataatcaag aaaaaagtct gactttctca agataaaaag
     2581 cctgagacca actcagactt tttaattctt aaaatggcaa ttcttcctct tccaagacca
     2641 aatctgccaa atcttggcct gcattatttt cacgcatagc acgttgggca cgactttcca
     2701 agagttggaa tcctgtcaca aggacttcgg ttacataatt catctgacca tttttctcaa
     2761 agcgacgggt acgcaattct ccatcaacgg aaatgagact acctttggtt gcgtagcttg
     2821 ccaaagtttc tgctagtctg ccccatagga ccatattgac aaaatcaact tcacgttcac
     2881 cgttttggtc tttgtaacga cggttgacag cgatagttgc tcgcgctact gacttgtcat
     2941 tgttggtttt gtgcaattct ggtgtagacg ttaaacgtcc aatcaagata actttattat
     3001 acatattttc ttcctcctac ttatctattc gtaggaaatc aaaaaaagtt acagaaattt
     3061 gtaacttttc gagaaaattt tttatttttt atgaaccatg aaacctgtcg cctgttgatt
     3121 ggccataatg gtcatatctg taatctgaac acgacgaggt tgactagtca catagactac
     3181 tgtatctgca atatcctgag cttgcaaagc ttctattcct tggtaaacgg acgcagctcg
     3241 ttctttatca ccatgaaaac gcactgtaga aaaatctgtt tcgacaattc caggctgaat
     3301 ggttgtcacc ttgatatccg ttgcgatggt atcaattcgc agtccatccg aaaaggtctt
     3361 aaccgcagcc ttggtagctg agtaaacagc ggcaccagca taggcgtaga ttcctgcggt
     3421 tgaccccata ttgataatat gaccttgatt gacttttatc attgctggca agaaacagcg
     3481 agtaaccgcc atcagacctt taacattggt atccaacatg gtcaacatat ccaactcctc
     3541 atagtcttga tagggagcca agtcaagagc cagtccggcg ttgttgacca agatatcaat
     3601 cctccctatc gtttctagaa tatcagagca aacagtcttc accatgacca tatccgtcac
     3661 atctagtgga aaagtccaga cggtttgatt tggaaagctt actgcaaact ccgacttgag
     3721 gatttctaat ctgtctatcc gtcgtcctgt tagaacgaca tcctcaccct gctccagata
     3781 agcacgcgca atcgcttcac cgattcctga ggttgctccc gtaatcacaa cattttttgc
     3841 catcttattt ccttctagct ggtctatcag atattaacaa cttcttaggc agtccagtgt
     3901 ttagctgagt caaatggtgt tccgacaact tggtcttctg ataattcaag caccccacgt
     3961 ttttgtggag catttggcag atgcaattca cgaggactgc acatcatacc aaaactcttt
     4021 tcaccacgaa gttcacctgg gaaaatgaga ttcccttttg gcatcatagc tccaggaagc
     4081 gcgacaatgg ttttcaaccc cacacgcgca ttgggagctc ctgcaacgat ttgtacagtc
     4141 ttatcacttg cgactgcaac ttggcagatg ttgaggtggt cactatctgg atgggctacc
     4201 atctcaacaa tttcaccgac aacaaactta ggttccttat cattaacaat ttcttctgta
     4261 aaaccttccg cctgcaactc ttggttcaaa cgagcgactt gctcatctgt caaaaagact
     4321 tgaccgcgct ctgcagtttc aaataaactt gaaacttcga aaatattcca agccactgtt
     4381 tccccattat ctttgagaaa aacacgggct accttgcctt tgcgctccac atccagtttg
     4441 gcatctccgc tatttttcac gatgaccata aggacatcac cgacatgttc tttattatat
     4501 gtaaaaatca ttgtttcctt tttctcctat ttcagtcctg ctaaaaagtc attgatttgt
     4561 tgcttgcttt tacggtcgcg attgacaaaa cgaccgattt ccttgtcctt ttctagaaca
     4621 acaaggctag gaattccgta aacatcccag agtttggcca aatccatata ctgatctcgg
     4681 tccattcgaa taaaggtgaa ctctggattg gtctcctcaa tctctggtaa ggcaggataa
     4741 atataacggc aatcgccaca ccagtctgcc acaaaaagga agaccttctt gcccgctttt
     4801 tccactaaag atgctaattc ttctaaactt gctggctgta tcataagact tcctcctcat
     4861 agactaggtc ttcattttca tagacaaagg tataatgacg gccatcctca aaaatgacgc
     4921 caccaaccaa gctctccaga ctgctttcgt aaacttgaac atagagggtc gcaatttccc
     4981 ccatgtcgga aaaatggtct cgcacaatct ctgtcaactc ttcctgagtc ttcatgagct
     5041 tacggtcatc tgcaactttt ttcgtagcaa gagcaaggct tccaatacct agcagagcca
     5101 agcctgccat ccacattttt ttagctttca taccattcat tttaacacaa aaaaggcttc
     5161 aggacaaatg aggaagcagc agaaaagcaa gtaaaaagcc tcttccttta aggaaaagga
     5221 cttcgtatac taagagcaaa ctaggaagct agccgcaggc tgctcaaaac acggttttga
     5281 ggttgtagat aaggcgacgc tgacgtggtt tgaagagatt ttcaaagagt attattctta
     5341 ttgccaggca cctaagttgc caacgtagta accatcaggt gtgtaggtat tgcgaaacat
     5401 cttacttgat gaagccagat aatactactt gccattgtct ttgacccaat cattcgcaat
     5461 catggaacca gaagaactta cataatacca ttctcccttg tcataaaccc aagtactgac
     5521 tttcatggtt cctgagcaat taaaggcaaa aaaactgtcc aatatcattc gttttttaaa
     5581 agcatttgac actacatcat ctacgctgta gtacatagat acatcaaacg taaaagccaa
     5641 aatatcgaaa aatatacaac ttttaaaggc agcatttcat tttttaaagt ctaacaacca
     5701 tagaactgaa atcagagcaa ggcatcaatt tcctactctt agtacgtcta ttttgatggt
     5761 tgaagtgaag atttttttat ttaacgtaga caagctccaa cttcccaaag gccacgtctc
     5821 cacgaataat caaggttttg cttgttggcg ctacaggagc gtccgccttg gctgcaccaa
     5881 aggatgtttc tactttcaaa tcgacacgcc agtgttgtgg aacatagacg gttgcatttc
     5941 cgaaggccac ttcaatattt aaagttgcaa aatcacctag catctctgca ttatcatagt
     6001 agattttagc atccccaaaa gcgacttcca cttggtcatc tacgagatct tgatcttgct
     6061 tatagaaggt cccgctacca aaagcgactt ccttatccgt gacgactgtt tttttcccat
     6121 tgtaccacca ttttttttca ttccagaact tacttgaatg cgtcagataa ccaacaccaa
     6181 gtaccaccaa gatgctagcc caaataagag aatgattggt aactggtaac aagtcataag
     6241 cgtaatttgc aatgatgagc gccagtaaac ctgtaaaaac tgccgaagtg agatgacgtc
     6301 taaggatgga ctcaatggac ttataagcaa aaaaaacaat acctagtaaa ggccatattt
     6361 taccatccaa agaaggaatt ccaaaattcc cttgcagcaa gatccaagct gctaaaacca
     6421 ataaaacaat accaaatgct ttctttttca tgttttttac ctcatgtttc ttagattttc
     6481 ttttaggagg gattggtaat gccgtgagac atgaacctcc ttgtgcgtct gataaaagga
     6541 aatggtgccc gttcctgaaa aggacttgtc cactgagtaa atctgacgga tgtttgcgat
     6601 cgttgacttg gaaactcgat taaaatagcg aggcaagata gactccaact catagagctt
     6661 gaggcgaacc tcataagctt ccttctggtt atgagcgtag atcttgctcc cttctgtttc
     6721 aaagaagaga atttctgaca ggtctagata atattcacct gtccccttgt aaaaagtcaa
     6781 cctcggagcc tttgactctt gcaagagccg ttgcaagtct gcaatttcat ctgtcaaagt
     6841 gggggtcttg atgacaattt cagtctcctc taaattgccg tcaatctcaa ttcgtaactt
     6901 catcacatct cctttctgac tctactatat caaagctaaa ttaggaatac aagggcaaaa
     6961 aagcaagtgg tcaatttgaa gcagtaagtg gttgtaggac tagcttaact tacatgtcag
     7021 aataggaaga aaaggaatcc ccacaccaaa ccacaagcat agccaaccaa gacatccttg
     7081 ggataatgaa caccccctaa aaccctcacc aaccccagca aagctgaaaa gatcaagcat
     7141 actaagccaa taggtagact agcatgcaga caggccatgg agatgatagt tgccgaaaag
     7201 acatggcgac tgggcatcga ccgtcccgaa ctatccttat caagaagggg acagatatcc
     7261 caagtttcat aaggtctcgg gtgattgatt ttcttacgaa acagggacaa ggccacaaaa
     7321 cctgatgcag gaataagcac ataaacccca acttgctgac caagtcctag ttgcaggtaa
     7381 gtggtgacta gcaagatcag atagaccagg ggcatggcga ctgtcatcaa acgattgaaa
     7441 ctgcgtaata ggaataaaag aattggacga ctagttagtc tggagctgat atttcgatac
     7501 cattcttgat aatttttcat ttatgcttct catacttttc tagaacttgt tgagatgttt
     7561 tccccttggc aagccaatcc actagcttgt ccaagcgacg tatttcctgc atcagaggat
     7621 catcaatttc ctcaatacgg acaccgcaaa tcttccctgt aattgcaatt cgttcaggga
     7681 cataatatgg tgcttgtcta aaaaaatctc cgtaagtaac atccctatcc agacaagcaa
     7741 gaacatcact cacttcatac cctgtcaacc aactggtcac ttggtgaact gactctgcct
     7801 ttccaccctt tcgctcaact tttgcaatca aggctaagta gacagcagca aatttcatat
     7861 tgtataattt ctgactcata cttctctctt tctatgtgta ctagtataaa taagaaaaag
     7921 aaggccgtca agccttcttt tgatttattc ttctgcttca tcttctgtaa attgactatt
     7981 gtacaagtca gcgtagaagc caccttgcgc catcagttcc tcatagttgc cttgctcgat
     8041 gatatttcca tctttcatga ccaagatcaa gtctgcattt cggatggttg acaagcggtg
     8101 ggcaatgaca aaggatgtgc gtccttccat caaacggtcc atggcttttt ggatcaattc
     8161 ctctgtccgt gtgtcaacag aagaagtcgc ctcatccaaa atcaaaagcg gtgcatcctt
     8221 aagaagggca cgagcaatag tcaatagttg tttttgtcct acagacaagg tcacggtgtc
     8281 atccaagatg gtatcatagc catctggcaa ggtcataata aagtggtgaa ttcccacagc
     8341 cttactagct tccatcattc gttcatcact aatccctatt tgattataga tgagattgtc
     8401 tcgaatagtt ccttcaaaga gccaggtatc ctgcaagacc attgaaaagg catcatgcac
     8461 ttctgaacgc gtcatagcct tggtatccac accatcaatg tgaatacttc ccttatcaat
     8521 ctcatagaat ttcatcaaaa gattgacaat ggttgtctta ccagtcccag tcggcccaac
     8581 aatggcaacc ttttgaccag catgagctgt cgcagagaag tcatagtctt gaacattgac
     8641 accgtccacc agaatttctc ctgctgacac gtcgtagaaa cgtggaatca gattgaccag
     8701 agttgattta ccagaacctg ttgacccaat aaaggccact gtttgaccag tttctgcttt
     8761 aaagctaaca tgttcaataa ctgcctccga atttgccgca tagcggaagg tcacatcctt
     8821 aaactcgacc tgacctttga agttttcatc agtcagctgt acttgaacag ggttttggat
     8881 agaagaatgc aaatctaaaa cttgattaat ccgcttagca gagaccatag ttcggggaag
     8941 aacgatgaag agtgctccca tgagaaggaa gcccatgaca acctacatgg cataagacat
     9001 gaaaataatc atgtcactaa agagaggcag acgcgctatc ggagcagcgt cgttaatcac
     9061 ataggcccca atccagtaaa tcgctacact caaaccactt gaaatcccca tcatgatagg
     9121 attcaaaata gccataagac ggttgacaaa caaattcaaa cgggtcaatt catcatttac
     9181 tgctgcaaat ttttcatttt gataatcctc tgcattgtag gcacgaacga cacgaatacc
     9241 tgt
//
LOCUS       DAVKGU010000079         9024 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKGU010000079 DAVKGU010000000
VERSION     DAVKGU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.080"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            186..1973
                     /locus_tag="VW077_001693"
     CDS             186..1973
                     /locus_tag="VW077_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9455679.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1970..2446
                     /locus_tag="VW077_001694"
     CDS             1970..2446
                     /locus_tag="VW077_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9455680.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2474..3379
                     /locus_tag="VW077_001695"
     CDS             2474..3379
                     /locus_tag="VW077_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9455681.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3366..4181
                     /locus_tag="VW077_001696"
     CDS             3366..4181
                     /locus_tag="VW077_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9455682.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4188..4592
                     /locus_tag="VW077_001697"
     CDS             4188..4592
                     /locus_tag="VW077_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9455683.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4845..6011
                     /locus_tag="VW077_001698"
     CDS             4845..6011
                     /locus_tag="VW077_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9455684.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6127..7164
                     /locus_tag="VW077_001699"
     CDS             6127..7164
                     /locus_tag="VW077_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9455685.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7372..8226
                     /locus_tag="VW077_001700"
     CDS             7372..8226
                     /locus_tag="VW077_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9455686.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWNIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            8376..8633
                     /locus_tag="VW077_001701"
     CDS             8376..8633
                     /locus_tag="VW077_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEU9455687.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDCDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8675..>9024)
                     /locus_tag="VW077_001702"
     CDS             complement(8675..>9024)
                     /locus_tag="VW077_001702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455688.1"
                     /translation="VVDGTDEVSSKHCFEVVDGTDEVSSKHCFEVVDRTDEVSNHIYG
                     KVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFLGSIHSTPDTMRFFNFK
                     DFLQLLLTHIKLF"
BASE COUNT         2571 a         1704 c         2039 g         2710 t
ORIGIN      
        1 tatattccga atttaaagag cgtttgaaat gggtctaaaa atgtttgaaa ctaattgaat
       61 gtgtgtataa tagtaaaaat gcagtaaata tcccgacaat ttctattgac aattcaaaca
      121 gattggttta taattaatat aacaacaaat gaaagcgcaa actttcgcgg tcggaaggta
      181 gttttatgac acgatttgag atacgagatg atttctatct cgatggaaaa tcatttaaga
      241 ttttatctgg tgccattcat tattttaggg ttcctccaga ggattggtat cattcgctct
      301 ataacttgaa ggctcttggt tttaatacgg tagagactta tgttgcttgg aatttacacg
      361 agcctcgtga aggtgagttt cattttgacg gtgatctgga tttagagaaa tttctccaaa
      421 tagcgcagga tttgggtctc tacgcaattg tgcgtccgtc tccatttatc tgtgcggaat
      481 gggaattcgg tggcttacca gcttggctct tgaccaagaa catgcgaatt cgctcatccg
      541 acccagcata tatcgaggca gttggtcgct actatgatca gttattgcca agactggttc
      601 ctcgtttgtt ggacaatggt ggcaatattc tcatgatgca ggttgaaaat gagtatggtt
      661 cttacggaga agataaggct tacctgagag cgattcgaca gctaatggaa gagtgtggcg
      721 taacctgtcc cctctttaca tcagatggtc catggcgagc tactctgaaa gctggaacct
      781 taattgaaga ggacctcttt gtaacaggaa actttggttc taaggcacct tacaactttt
      841 cgcagatgca ggaattcttt gatgaacatg gtaagaaatg gccactcatg tgtatggagt
      901 tctgggatgg ttggtttaat cgttggaaag aaccgattat cacacgggat cctaaggaat
      961 tggcagatgc agttcgagaa gttttggaac aaggctccat caatctttac atgttccacg
     1021 gtggtacaaa ctttggtttc atgaatggtt gctcggctcg aggaactttg gacctgccac
     1081 aagttacgtc ttatgattac gatgcccttc tggatgaaga aggaaatcca actgctaaat
     1141 atcttgcagt caagaagatg atggcaacac atttttcaga gtatccgcag ttggaaccac
     1201 tctacaaaga gagtatggag ttggatgcta ttccactagt tgaaaaagtt tctttgtttg
     1261 aaaccttaga tagcttgtca agtcctgtag aaagtctcta tcctcaaaag atggaggagc
     1321 tgggacaaag ttatggctac ctactttatc gaacagaaac aaactgggat gcagaagaag
     1381 aaagacttcg tatcattgat ggtcgagata gggcccagct gtatgtcgat ggtcagtggg
     1441 ttaaaactca atatcagaca gagattgggg aagatatttt ttatcaaggt aaaaagaaag
     1501 ggctatctag gttagatatc ttgatagaaa atatggggcg tgtcaactat gggcataagt
     1561 tcttagcgga tacgcaacgt aagggaattc ggacaggggt ctgtaaggat ctgcatttct
     1621 tactaaactg gaaacactat ccactcccac tagacaatcc tgagaaaatt gatttttcaa
     1681 aaggatggac tcaaggacaa ccagcctttt acgcttatga ctttacagtc gaagagccaa
     1741 aagatactta cctagacttg tctgagtttg gtaagggggt tgcctttgtc aatgggcaga
     1801 atctaggacg tttttggaac gttggcccaa ctctctcact ttatatccct catagctatc
     1861 tcaaggaagg tgccaaccgc atcattatct ttgaaacaga aggtcaatat aaagaagaga
     1921 ttcatttaac tcgtaaacct acactaaaac atataaaggg ggaaaactta tgacaattgt
     1981 aggatgccgt attgatggac gtttgatcca cggacaagta gccaatcttt gggctggaaa
     2041 actaaatgtt tcacgcatta tggttgtaga cgacgaagtt gtcaacaacg atattgaaaa
     2101 gagtggtttg aaacttgcga caccaccagg tgtgaaattg agtattttgc cagttgagaa
     2161 agctgcagcc aatattcttg ctggcaaata cgatagccaa cgtctcttta tcgtggctcg
     2221 taaaccagac cgcttccttg gtttggtaga agcaggtgta ccacttgaaa cccttaatgt
     2281 tgggaatatg tctcaaacac cagaaactcg ttctattaca cgttctatca acgtagtaga
     2341 caaggatgtg gaagacttcc acaaactggc agaaaaaggt gttaaactta ctgctcagat
     2401 ggttccaaat gatccaattt cagacttttt gagcttatta aaataggaaa aaatttttta
     2461 ggaggtcatt gttatgatac aatggtggca aattttactt ctcactttgt actcagctta
     2521 tcaaatctgt gatgagttga cgatcgtttc atctgcaggt tcccctgtat ttgctggttt
     2581 cattactggt ttaatcatgg gagatgtgac tactggttta cttatcggtg gtaacttgca
     2641 actgttcgtt cttggggttg gtaccttcgg tggtgcttct cgtatcgacg caacttctgg
     2701 tgcggttctt gcgacagcct tctctgtttc acaaggaatt gatgcaccgc ttgcaattac
     2761 tacaatcgct gtaccagtag cagctctctt gacttacttc gacgttcttg gtcgtatgac
     2821 tactaccttc ttcgctcacc gtgtggatgc tgcaatcgaa cgctttgact ataaaggtat
     2881 tgaacgcaac tacttgcttg gtgcgattcc gtgggctcta tctcgtgccc ttccagtctt
     2941 ctttgccctt gcttttggtg gtgcctttgt acaatcagta gtagacttcg ttgaagccta
     3001 caaatgggtt gcagatggct tgacactcgc aggacgtatg cttccaggtc ttggatttgc
     3061 aatcttgctt cgttaccttc cagttaaacg taaccttcac taccttgcta tgggatttgg
     3121 tttgacagct atgttgactg ttctttactc atatgtaaca ggtcttggtg gcgctgttgc
     3181 tggtatcgta ggtactcttc ctgctgaagt tgctgaaaaa attggtttcg tgaacaactt
     3241 caaaggtttg tctatgattg gtatttctat cgtaggtatt ttccttgcag tgcttcactt
     3301 caaaaatagc caaaaagtag ctgtagcagc accttctaca ccatcagaaa gtggggaaat
     3361 cgaagatgac gaattctaat tacaaactta caaaagaaga ttttaatcaa atcaacaaac
     3421 gtagcttgtt tactttccaa ttaggttgga actacgaacg tatgcaagct tctggttacc
     3481 tttacatgat cttgcctcag ttgcgtaaaa tgtatggtga tggaactcct gaattgaaag
     3541 aaatgatgaa agttcatact caattcttca atacttcacc attcttccat accattatcg
     3601 ctggttttga ccttgccatg gaagaaaaag atggtgtagg ttcaaaagac gctgttaacg
     3661 gtatcaagac aggtttgatg ggaccattcg ctcctcttgg ggatacaatc tttggttcac
     3721 ttgtacctgc tatcatgggg tcagtcgcag caactatggc tatcgctggc caaccttggg
     3781 ggatcttcct ttggattgca gttgcagtag cgtatgacat cttccgttgg aaacagttgg
     3841 aatttgctta caaagaaggg gttaacctta tcaacaacat gcaaagtacc ttgacagctt
     3901 tgattgacgc tgcatctgta cttggtgtct tcatgatggg tgctcttgta gcaacagtga
     3961 ttaactttga aatttcttac aagttgccaa tcggtgaaaa gatgattgat ttccaagaca
     4021 tcttgaacca aatcttccca cgtttgcttc cagcaatctt tactgccttt atcttctggt
     4081 tgcttggtaa aaaaggtatg aactctacta aagctatcgg tattattatc gtacttgctt
     4141 tggctctttc tgcccttggt cactttgcac ttggaatgta attccttatg actaaatcat
     4201 taattttggt gagccatggt cgcttctgtg aggagcttag aggtagcaca gaaatgatta
     4261 tgggcccaca agacaacatt tacacagtag ctcttcttcc agaagatggc ccagaagaat
     4321 ttactgctaa atttgaagct gttattgaag gattggatga tttcctagtc tttgcggatc
     4381 ttctcggtgg gacaccttgt aatgtggtga gtcgcttgat catggaaggt cgtgatattg
     4441 acctttacgc agggatgaat cttccaatgg tgattgaatt tatcaatgcg agccttacag
     4501 gcgcagatgc ggactacaag agccgtgctg cagaaagcat tgtgaaagtt aatgacctgt
     4561 tagcgggctt cgatgatgac gaagatgaat aatactcttc gaaaatctct tcaaactacg
     4621 tcaacgtcgc cttgccgtag gtatatgtta ctgacttcgt cagtcttatc cggcaacctc
     4681 aaagcggtgc tttgagcagc ctgcggctag tttcctacag attttagttg gaactcgatt
     4741 caattcatgt gacaacgtga aaatcgttag agcattttat atagaatata catgggaatg
     4801 gagcttactc ccattcccat atttaataga aaaagaggaa ctcaatgcta cattatacaa
     4861 aagaagactt gctcgaattg ggtgcagaaa tcactacgcg tgaaatctac caacagcctg
     4921 atgtatggag agaagctttt gaattttatc aagcaaaacg tgaagaaatt gcagcctttc
     4981 tacaagaaat cgctgataaa catgactata ttaaggttat cttgacaggt gctgggactt
     5041 ctgcttatgt gggagatacc ttgctacctt actttaagga agtctatgac gaacgcaaat
     5101 ggaatttcaa tgctattgcg acaacagata tcgttgccaa accagcaacc tatttgaaaa
     5161 aagatgtggc aactgtcctt gtgtcttttg ctcgtagtgg gaactcgcct gaaagtgtgg
     5221 cgactgttga tttggccaaa tccttggtgg atgagcttta tcaagtgacg attacttgtg
     5281 cagcagatgg taaattggct cttcaagctc acggtgatga ccgtaatctc ttgctcttgc
     5341 aaccagctgt ctctaatgat gctggatttg ccatgacttc tagctttacg tctatgatgt
     5401 tgacagctct cttggtcttt gatcctacag aatttgctgt taagtctgaa cgttttgaag
     5461 ttgtatctag tcttgcccgt aaagttttag acaaggtaga agatgtcaaa gagctcgttg
     5521 atttagactt taaccgtgtc atctatctag gcgctggtcc tttctttgga cttgctcatg
     5581 aagctcagct caagattttg gaattaactg ctggtcaagt tgcgaccatg tatgaaagcc
     5641 cagttggctt ccgtcacggt ccaaaatctc ttatcaacga caatacagtt gttttggtct
     5701 ttggtacaac gacagcctac actcgtaagt acgacttgga cttggttcgt gaagttgctg
     5761 gtgaccagat tgctcgtcgt gttgtgcttt tgagtgatca agcttttggt cttgaaaatg
     5821 tcaaagaagt ggtccttggt tgtggcggtg tcttgaatga tatttaccgt gtcttccctt
     5881 acatcgttta tgcccaactc tttgctctat tgacttcact caaggtagaa aataaaccag
     5941 atacaccgtc tcctacaggt acagtaaacc gtgtagtaca aggtgtcata attcacgaat
     6001 atcaaaagta agacagtgtt tatgaattct tgacaagagg atttgtaaat tatcagataa
     6061 accatagatt gtcagtacgc tttctatggt ttgtttgctt gagagaaata gtaaaaggag
     6121 aacagaatga aagcatacac agagcgtgta tttggaaatg ttgagggcga ggatgtcttg
     6181 gcctatcgat ttgagacaga cggtggctac caacttgagg ttatgactta tggcgcgacc
     6241 atcttgcgct atgtcgcacc tgacaaggct ggaaattttg ccaatgttat cttgggattt
     6301 gatgactttg atagttatgt aggcaatagt cccaagcatg gagcaagtgt aggtcctgta
     6361 gcgggtcgta ttgcaggtgc gacctttgag ctcaatggta agacctatga ccttgaggtt
     6421 aataatgcta gcaactgtaa tcacagtggt tcaactggtt gggattccag cttgtttgaa
     6481 gttgaagaag taagcgatca tggcttgact ctctacacag agcgtacaga tgggacagga
     6541 gggttccctg gaaatctcaa gatttggatc agttatcact tggaagaaac tggtgcctat
     6601 gaaatcatct acaaggtaac gaccgatcag gatacgctgg tcaatccaac caaccacagc
     6661 tatttcaact tgtctggtga tttcacgcag acgattgacc gtcatgtctt ccaactaaac
     6721 acagagggca tttactcaat cgctcctgac ggtgttcctg ccaaaactcc agaagccaac
     6781 cgtgatgtgg tcaaacacat ctacaatggt accttgttga aggatatctt tgcagaagaa
     6841 gatgagcaaa tccagctggc atcaggtttg gatcacccat ttgcccttcc tgcaggccat
     6901 gacaatgctg gattccttta tgaccaaaat tcaggtcgct tcctgctttt caagacagaa
     6961 gctccttgct ttgtggtcta cacagcaaac tttgtggatg aaagtgtcat cataggaggt
     7021 cagccaatgc tacagcacaa tgggattgct cttgaagcgc aagctttacc agatgccatt
     7081 cacagtgacc ttaaaggcca agtcattctt aaagctggtc aaaccttcac cagtaagaca
     7141 cgttatgaac ttgttgtgaa ataaaaaaag ctggtcaaaa agtctttaaa attaaaataa
     7201 cgcatattgt caggtgatga aaagtttgat aatatgcgtt ttattatgaa atgatttact
     7261 ttatcttatc ctgaaatggt gttatttttg agttatctgg cttgactttc ttgatggaaa
     7321 ttatataata gttgtaataa caataattac aacgagaaaa gaggaccatt tatgacctat
     7381 gaatacaaga gtcacattta tttggcagag acagttttaa atgtaaagga tttggccagt
     7441 caaacagcct tttatcagca agttattggt ttagaaatct tatctcaaac tgagacagag
     7501 tccattctag gccttggtgg aaaagtcttg gtacagctga ttcaagcaca agagagcgga
     7561 gaagtgaggg aacattatgg tctttaccat ttggccattc tcttgcccac acgcaaggct
     7621 ttggctgatg tcttgaaaca cctgacagat ttgcaaattc ctcttgtcgg cggtgcagac
     7681 catggttaca gtgaagctat ttacttggaa gatttggagg gaaatggcat tgaactctat
     7741 cgagataagc cagtttccac atggaatatt cgagaagatg gacgcattat cggagtgacg
     7801 gaggctcttg cggcgcagga catttatgag ttgggggaaa gagtagagcc ctttatcctg
     7861 gcagaaggta caagaatggg gcatattcat ctttccgtca aggatagtcg aaagtccagc
     7921 cagttttatc aaaaggtgtt agggctagag aataaattca gtgtgcctgg cgctagttgg
     7981 attgcggctg gagattacca tcatcattta gcagttaacg aatggggagg aaaaggtctg
     8041 gcttcgcgta agcaaggctt accaggttta gcctactatg tcatcgaagt cgcacataaa
     8101 aaagaactgt taacgattgc ccaacgagca caagaagttg acgcaccaat caaatggatg
     8161 acatcgatcc aattggaaat cacagactca gatggcatcg tgacccgtat tcgtttagca
     8221 aggtaaaatt aggaattttt aataagttat aagtattgct agtttgcttt ttgaacggtc
     8281 tttggtaaca gaactgcgtg agctggatgg gtgagaattt tgacaaagaa gctgattaga
     8341 gatataatga agaaaaatcg agcaaggaaa aagaaatgac aaattcaaag tatattactt
     8401 gtttgaaacg ttcagagggc cagttgtgtg ggattcaaaa gatgattgaa ggagattgtg
     8461 actgtgctga tattgtgacg caactgacag cagtgagatc tagtgtagag cgcgtgattg
     8521 agatgataat taccgaaaat cttactgaat gtatcaatca gccactagat gattctgaag
     8581 ctcaaaagga acgcctagaa aaggctatcc gatacttgat taaacggaaa taaatgaatg
     8641 ttttgagacg atatgtgcaa aggtctatta actattagaa tagtttaatg tgagtcaaaa
     8701 gaagttgtaa aaaatcttta aaattaaaaa aacgcatagt atcaggtgtt gaatgtattg
     8761 accccaaaaa gttagatttt ttctgtctaa cttttggggt gcagttcata agaaccttgg
     8821 taatatgcgt tttattatgt agagacttat actcttcgaa aatctcttca aaccacgtca
     8881 gcttcacctt gccgtagata tggttactga cttcgtcagt tctatccaca acctcaaaac
     8941 agtgttttga gctgacttcg tcagttccat ccacaacctc aaaacagtgt tttgagctga
     9001 cttcgtcagt tccatccaca acct
//
LOCUS       DAVKGU010000080         8965 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKGU010000080 DAVKGU010000000
VERSION     DAVKGU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.081"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            168..323
                     /locus_tag="VW077_001703"
                     /pseudo
     CDS             168..323
                     /locus_tag="VW077_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            523..3798
                     /gene="rexB"
                     /locus_tag="VW077_001704"
     CDS             523..3798
                     /gene="rexB"
                     /locus_tag="VW077_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9455689.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLEYLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFFEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3795..7445
                     /gene="addA"
                     /locus_tag="VW077_001705"
     CDS             3795..7445
                     /gene="addA"
                     /locus_tag="VW077_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9455690.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGCQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQITILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRNRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKAHENLYEKLVNAQKKAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7660..8838
                     /locus_tag="VW077_001706"
     CDS             7660..8838
                     /locus_tag="VW077_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455691.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2771 a         1872 c         1899 g         2423 t
ORIGIN      
        1 aagattatac cacattgtgt cctatttttg gttcatttta ctatagtaga ttgaaataag
       61 atacgaacag cttgattagg gaaatcaaat taatttctag aaatatttta gcatccacgg
      121 tgtactgtta tagtttcaat ttactatagt tcaatcattc tatcaagaat attgaatata
      181 taaaggcacg taatattaaa acttcttctg atatcgaatt agagtccgat gttgatggcg
      241 ataaatcaga caatctaccc gtcgacatta agatactggg acaacacatt gaagtgtatt
      301 ccgtacctaa gaaacaatca taaaactcaa agccttgtta ccgaggcttt tttattatgt
      361 gattttcagt agccagactt attctatcta atacaagtaa agctaagtat ttaagattta
      421 gcagatgatg aagaaaagaa aggatggcct aaatatttta acagatttac gaggctaatc
      481 atggtataat atagggtgat gaaaaattcc aacgaggctg agatgaaatt actttatact
      541 gatattcgga cttctttgac agaaattcta acaagagagg cagaagagct agttgcagct
      601 ggcaagcggg tcttctacat tgcccccaac tctctttctt ttgaaaagga acgcgccgtg
      661 ctggaatact tgtcccagca ggcttctttt tcgattaccg tcacgcgctt tgctcaaatg
      721 gctcgctatc tggtcttgaa tgatttacca gctaaaacta ctcttgatga tatcggtctt
      781 gggttggcct tttacaaatg ccttgccgaa ctcgatccca aggacttgcg tgtttatggc
      841 gctattaagc aggatcctca attgatccag cagttaattg agctttacca tgagatgacc
      901 aaatctcaga tgagtttttt ggacttggag aatttaacag atgaggataa gagggcggat
      961 ttactcttga tttttgagaa agtaacagcc tatcttaatc aaggtcagtt agcccaggga
     1021 agtcagttgt cccatttgat tgaggctatt gagaatgaca aggtaagtag tgattttaat
     1081 caaatcgcct tggtcattga cggctttact cgtttttctg ctgaggaaga gcgggttgtg
     1141 gacttacttc acggcaaagg tgttgagatt gttatcgggg cttatgctag taagaaagcc
     1201 tataccagtc cttttagcga gggcaatctc taccaagcca gcgtaaaatt tctccatcat
     1261 ctggcttcta aataccaaac gcctgctcag gactgttctc aaactcatga gaagatggat
     1321 agttttgaca aggcctctcg tttgttggag tcttcttatg acttttcaga actcgctttg
     1381 gatgtcgatg agaaagaccg tgaaaattta caaatctggt cttgtttgac gcaaaaggag
     1441 gagttggagc tagtagcccg tagtattcgt cagaaattac atgagaactc agacctgagc
     1501 tacaagcatt ttcgtattct cttgggggat gtagcttctt accagttatc tctcaaaacc
     1561 atttttgacc agtatcagat tcctttttat cttggtagaa gcgaagccat ggctcatcat
     1621 cccttgactc agtttgtcga gtctatttta gctttaaaac gttaccgttt tcgtcaggag
     1681 gatttgatta atcttcttag aactgatttg tatactgacc tcagtcagtc tgatattgat
     1741 gcttttgagc aatatatccg ctatcttggt atcaatggct tgccagcctt tcagcaaacc
     1801 ttcaccaaat cccaccatgg aaaatttaat cttgagcgtt tgaatgtcct ccgcctgaga
     1861 attttagcac ctcttgaaac cctctttgcc agccgaaaac aaaaggctga aaacctccta
     1921 caaaaatgga gtgtctttct aaaagaagga gctgtgacca agcagttaca agatttgaca
     1981 accactttgg aagctgtaga acaggaaaga caagccgaag tttggaaggc tttctgccat
     2041 gttttagaac aatttgcgac tgtttttgct ggttcacagg ttagtctgga agacttccta
     2101 gccttgctcc attctggaat gagtttgtcc caataccgta ccattccagc aacagtggac
     2161 actgttctgg tgcagagtta cgatttgatt gcaccattga ctgctgactt tgtctatgct
     2221 attggactaa ctcaggacaa tttaccaaaa atttctcaaa acaccagtct tctgacagat
     2281 gaagaaaggc aaaatctaaa ccaagcgacc gaagaaggcg ttcaattact gattgccagc
     2341 agtgaaaatc tcaagaaaaa tcgctacact atgctttcct tggtcaattc tgctcgtaag
     2401 cagttgttct tgtcggctcc aagccttttt aacgaaagtg aaagtaagga atctgcctat
     2461 cttcaagagt tgatccattt tggatttagg cggagagaga agaggatgaa tcacaaagga
     2521 ctgtctaagg aggatatggg gtcctatcac agtcttttgt ctagtctggt tgcctatcac
     2581 cagcagggtg agatgagcga tactgagcaa gatttgactt ttgtcaaggt tctgtcgcgt
     2641 gtcataggta aaaaactaga tcagcaaggt ctggaaaatc cagccatccc aatcagtcca
     2701 agcagcaaga ccttagccaa ggacaccttg caagctctct atcctgccaa acaggagttt
     2761 tacctgtcta cgtcgggttt gacagagttt tatcgcaatg aatacagtta tttcctacgc
     2821 tacgttttag gcttgcagga ggaattacgt ttgcgtcctg atgcccgtag tcacgggaat
     2881 ttcttgcatc gtatctttga acgcgccttg cagttgccta atgaagattc ctttgaccaa
     2941 cgtctagaac aagctattca agaaaccagt caagaacgcg aatttgaagc tatttatcaa
     3001 gaaagtttgg aagcccagtt taccaaggaa gttttgcttg atgttgcacg gacaactgga
     3061 catattctcc gacacaatcc agccatcgaa accatcaaag aagaagcaaa ttttggtgga
     3121 aaagaccaag cctttattca attagacaat ggacgcagtg tctttgtacg aggcaaggtg
     3181 gaccggattg accgtttgaa agctaatgga gcgataggag tagtagacta caaatccagt
     3241 ctgactcagt tccagtttcc tcatttcttt aatgggctca attctcagtt accaacctat
     3301 cttgctgccc taaaaagaga aggggagcag aactttttcg gcgccatgta cttggaaatg
     3361 gctgaacctg tccaatctct gatggcggta aaaagtctgg caggagcagt ggtagaagcc
     3421 agcaaatcta tgaaatacca agggctcttc tttgaaaaag aaagcagtta tttaggcgaa
     3481 ttttataaca aaaacaaggc taatcaactg acagatgagg aatttcagct cctactggac
     3541 tacaatgcct atctttacaa gaaagctgct gagaagattt tagcaggccg gttcgccatc
     3601 aatccttata ctgaaaatgg cagaagcatt gccccatacg tccagcaaca tcaggctatt
     3661 acaggctttg aagccaatta ccatctgggc caagcccgtt tcctagaaaa gttggaccta
     3721 gctgatggca agcgtctggt cggagaaaaa ctcaagcaag cttggcttga aaaaataaga
     3781 gaggagttga atcgatgaag cttattccct ttttaagtga ggaggagatt caaaaactgc
     3841 aagaagcaga agcaaattcg agcaaggaac agaagaaaac tgccgagcaa atcgaagcta
     3901 tctacacttc tggccagaat atcctggtct cagcatcggc tggttctgga aagacctttg
     3961 tcatggcaga gcgcattctg gaccaattgg cgcgtggtgt cgaaatttct caactcttta
     4021 tctcaacctt taccgtcaag gctgcaactg aacttaaaga acgtttagag aaaaaaatca
     4081 gcaagaaaat ccaagaaaca gatgatgtcg atctcaaaca acacttgggt tgccagttgg
     4141 cagacctacc caacgctgcc atcggaacca tggactcttt cacacaaaaa ttccttggca
     4201 aacatggtta tctgcttgat attgcaccta atttccgtat tttacaaaac caaagcgagc
     4261 aacttcttct cgaaaacgaa gtctttcatg aggtctttga agcgcattac caaggtaaac
     4321 agaaagagag ctttagtcat ttgctgaaaa actttgctgg gcgtggcaag gacgaacggg
     4381 gtctgcgcca gcaagtctat aaaatctatg acttcctcca atccaccagt aatcctcaaa
     4441 agtggctgag tgaatctttc ctcaaaggct ttgagaaagc tgattttacc agtgaaaaag
     4501 aaaaactgac tgagcaaatc aaacaagccc tctgggattt ggaaagcttt ttccgttacc
     4561 atctggataa cgatgccaag gagtttgcaa aggctgccta tttagaaaat gttcaattaa
     4621 ttctggatga aattggctcc ctaaatcagg agttcgatag tcaggcttat caggcagtgc
     4681 ttgcgcgtgt tgtcgccatc tctaaggaga aaaacggtcg agctctgact aatgccagcc
     4741 gtaaggctga tttgaagccc ctggctgatg cctacaacga agagagaaag acccagttta
     4801 ctaaactagg acaattatca gaccagataa ccattctcga ctatcaagaa cgttatcatg
     4861 gagacacttg gaaactagct aaaaccttcc aatctttcat gagcgatttt gtagaggctt
     4921 atcgtcagag aaaacgacag gaaaatgcct ttgaattcgc tgatatcagc cattacacca
     4981 ttgagatttt agaaaatttc ccacaagttc gtgagtctta tcaggagcgc ttccatgaag
     5041 tcatggtcga tgagtatcag gataccaacc atattcaaga acggatgctg gaattgttgt
     5101 ctaatggcca caatcgcttt atggtgggag atatcaagca atccatctat cgtttccgtc
     5161 aggcagaccc gcagattttc aatgagaaat tccaacgcta tgcgcaaaat cctcaagaag
     5221 gcaagctcat tctcctcaag gaaaatttcc gtagtagttc agaagtgctg tcagcaacca
     5281 atgatgtctt tgaacgtctc atggaccaag aggtcggcga aatcaactat gataacatgc
     5341 accagcttgt ttttgccaat accaaactga ctcccaatcc agacaacaag gccgaatttc
     5401 tcctctacga caaggacgat acaggtgagg aagaagagag tcaaacagaa acgaaactaa
     5461 caggcgaaat gcgcttagtt atcaaggaga tccttaaact tcatcaggaa aaaggtgttg
     5521 cctttaagga aattgccctt ctgacctcta gtcgtaatcg taatgaccaa attctacttg
     5581 ccctgtctga gtacgggatt cctgtcaaaa ctgacggaga gcaaaacaat tatctccaat
     5641 ccctagaagt gcaagtcatg ctagacactc ttcgtgtcat tcacaatccc ctgcaagact
     5701 acgccttggt tgcccttatg aagtctccaa tgtttggttt tgatgaggat gagctagcac
     5761 gtttgtccct tcagaaagca gaggataaag cccacgaaaa tctctatgag aaactggtca
     5821 atgcacaaaa aaaggcaagt agtcaaaaag gcttgattca cacagctcta gctgaaaaac
     5881 taaagcaatt catggatatc ctagcttctt ggcgcttgta tgccaaaccc cactctctct
     5941 atgacttgat ttggaagatt tacaacgacc gtttttatta tgactatgtt ggggctttgc
     6001 cgaatggtcc tgctaggcag gccaatctct atgccctagc actgcgtgct gatcaatttg
     6061 aaaagagcaa tttcaaaggt ttgtcgcgtt ttattcgtat gattgaccaa gtcttagaag
     6121 cccagcacga tttggcaagc gtggccgtcg caccgccaaa agatgcagta gagctcatga
     6181 ccatccacaa gagtaaaggg ctggagtttc cttacgtctt tatcctcaat atggatcaag
     6241 atttcaacaa gcaggactct atgtcagaag tcattctcag tcgtcagaat ggtcttggtg
     6301 tcaaatatat tgccaagatg gagacagggg cagtagaaga ccactatcct aaaaccatca
     6361 aactctccat tcctagtctg acctataggc agaacgaaga ggaattacag ctagcaagct
     6421 attctgagca gatgcgtttg ctgtatgttg ctatgacgcg ggctgagaaa aagctctatc
     6481 ttgtcggcaa gggttctcgt gaaaagctgg aagctaagca atacccagca gccaaaaatg
     6541 ggaaactaaa tagcaatact agactgcaag cacggaattt ccaagattgg ctttgggcta
     6601 tcagtaaagt gtttactaag gacaagctca actttagtta tcgttttatt ggcgaagatc
     6661 agttgaccag agaagctatc ggagagttgg aaaccaagag tcctctccaa gatagctccc
     6721 aagcagacaa tcgtcagtca gataccatca aagaagctct ggaaatgctg aaggaggtgg
     6781 aagtttataa tactcttcac cgcgcagcta ttgaacttcc tagtgttcaa accccaagtc
     6841 aaatcaagaa attctacgaa ccagttatgg atatggaagg tgtcgagatt gctggtcaag
     6901 gtcagtcagt aggcaagaaa atcagcttcg atttgccaga tttttcaacc aaagaaaagg
     6961 taactggagc tgagattggt agtgctactc acgaactcat gcagataatt gacctcagcc
     7021 agcaactaac ccttgctagc ctaacagaaa cactcaaaca agttcaaact agccaagctg
     7081 tcagagacaa gatcaatctt gataaaattc ttgctttctt tgacacagca ctcggtcagg
     7141 aaattcttgc taataccgac catctctatc gcgagcaacc tttctccatg ctcaaacgag
     7201 accaaaagag tcaggaagac tttgttgtcc gtggtatcct tgatggctat ctgctttacg
     7261 aaaacaaaat tgttctgttc gactacaaga cagaccgcta tgatgaacca agtcaactcg
     7321 tagaccgcta tcgtggtcag ttagctctat acgaagaggc tttatcacga gcctattcga
     7381 ttgaaaatat tgaaaaatac ttgattttac tcggtaaaga cgaggttcaa gttgtaaaag
     7441 tataacttat actcttcgaa aatctcttca aaccacgtca gcttcgcctt gctgtatata
     7501 tggttactga cttcgtcagt tctatccaca acctcaaaac agtgttttga gctgacttcg
     7561 tcagttctat ctacaacctc aaaacagtgt tttgagctga ctgcggctag ctttctagtt
     7621 tgctctttga tttttattga gtattagaaa ggagacctca tgacacttcc agttagaaaa
     7681 tccctgcacg atgcagtttt acaggcttcg aaagcggata cttgggaaca agctaccaag
     7741 gaatggaatg aagtttcctt gatttttaac ggtattggcc gtagcaattg tgtctgtgga
     7801 aatgccatca aatacgctta tgaactcttt aacggtgtca caggccaacg cctctttcca
     7861 attggtagcg actgtgttcg tcattttcat cgattgtccc ttgaccagca attggaagag
     7921 gaagaaaaac tgctcagaaa ggttgaaaat ctaaccagaa aagctcagaa aaaggaaaaa
     7981 atcaaggtca ataaaagtga ctttgacgag cgacttctaa aatggctctg ggaaaaaggt
     8041 gttttcaaac ccaatcgtgg caatcaattt gcgcctgaga gagactacca gcttttccta
     8101 gaagtctttc agggaggaag ttggaccaag gcggaaccaa agaaaaaggc tcggatggaa
     8161 gaagtccttg aaaagtgtat caaacccttt ttacttggga aatcggatga ccaactctac
     8221 cttgtcaagc taggcaagga gaaaattgac tacgaacaag aactccgtat ccaggcagag
     8281 aaagaacgta agaagaggga taaaatcgcc aagcaatatg cagacaatct ggttctagcc
     8341 atgggacccg cagagcgtgc ttatcaagat tactttggct ttacagagac cctgactcaa
     8401 gaagaacgca agtgggaaaa aattcttttt ggtaaaaata gagcagaacg ggctatcaag
     8461 gccaaacaat accaaaaaga gctggaaaaa gaccaacgaa ttgccagtca ggatccaatt
     8521 gaaagaaaac agaagcagac ttggctcctc aattcctatt ttcgtgagct tcctgaagaa
     8581 aaagctcgat tttctaggct cttattagaa tatcgaaaaa atggtgaagt acccttttca
     8641 actgaatacc tgtcagatca tctcatcgac tttttctata aaatgaaagc ctttgaattt
     8701 gaaatcgcac cagaacaagt ccgagatttt ctaaaaaaaa gcctgcagga agaccatcgc
     8761 tcatcagcac aaggaagctg gatagaagga attctcctca actgcctcaa accattttta
     8821 gaacgattag tgatatgagt aacacctacc gtgtaaattt catggtaggt gttattgtac
     8881 ccccaaataa tataaaacaa agtatgaaag cgcatctcaa ttttaagact tttttgtaaa
     8941 ttaagagtat aattaaggta tactg
//
LOCUS       DAVKGU010000081         8839 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKGU010000081 DAVKGU010000000
VERSION     DAVKGU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.082"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            89..541
                     /locus_tag="VW077_001707"
     CDS             89..541
                     /locus_tag="VW077_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9455692.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(576..1964)
                     /locus_tag="VW077_001708"
     CDS             complement(576..1964)
                     /locus_tag="VW077_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9455693.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            2030..2992
                     /locus_tag="VW077_001709"
     CDS             2030..2992
                     /locus_tag="VW077_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9455694.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPYIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(3021..3404)
                     /locus_tag="VW077_001710"
     CDS             complement(3021..3404)
                     /locus_tag="VW077_001710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455695.1"
                     /translation="MNTIERTRRLIKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETNEVSSKHVFEVVDETNEVSSKHVFEVVDRTDEVSSKHVFEVVDRTDE
                     VSSKHVFEVVDETDEVSNHTYGKAT"
     gene            complement(3468..4478)
                     /gene="ccpA"
                     /locus_tag="VW077_001711"
     CDS             complement(3468..4478)
                     /gene="ccpA"
                     /locus_tag="VW077_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9455696.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(4903..5502)
                     /locus_tag="VW077_001712"
     CDS             complement(4903..5502)
                     /locus_tag="VW077_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9455697.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLMLQPDVESVFQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERNIADLMQTLHTVLEGRKEYSPELMEVVMMHPNPLTEQEIAVLKGIAQGFSNQEIA
                     DKLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(5503..6600)
                     /locus_tag="VW077_001713"
     CDS             complement(5503..6600)
                     /locus_tag="VW077_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9455698.1"
                     /translation="MLERLKRIHYMFWISLIFMIFPILSVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILTFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVQVENHLDKSSLTQEL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVRDDGCGFASISGDELHTVR
                     NRVFPFSGEVSVISQKHPTEVQVRLLYKERK"
     gene            complement(6600..7337)
                     /locus_tag="VW077_001714"
     CDS             complement(6600..7337)
                     /locus_tag="VW077_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9455699.1"
                     /translation="MKRWLALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTGFSMMSTAIFSFPAVLHTDKMNNWQKILRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLILGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPVSLFPDWMQAIGKRLPSYQLMELIKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(7339..8223)
                     /locus_tag="VW077_001715"
     CDS             complement(7339..8223)
                     /locus_tag="VW077_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455700.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEVDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLVQAGCGIQEIEV
                     NNRSLLDTIFEETQKGDN"
     gene            complement(8210..8404)
                     /locus_tag="VW077_001716"
     CDS             complement(8210..8404)
                     /locus_tag="VW077_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455701.1"
                     /translation="MKKGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(8401..8607)
                     /locus_tag="VW077_001717"
                     /pseudo
     CDS             complement(8401..8607)
                     /locus_tag="VW077_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2674 a         2032 c         1563 g         2570 t
ORIGIN      
        1 aatattcttt tgaaaaatac tttttttgct ttacttctat agtgaaaagg tttacaatta
       61 tagtaagaaa atttcaggag gtttcattat ggcacaacgt taccaaaata tcatggtcgc
      121 aatcgatggt tctaaggaag cggacttggc ttttgtcaag ggagttcatt ctgctctacg
      181 aaacgacgct aaactcacca tcgcacatgt cattgacaca cgcgctctcc aaagcgtatc
      241 cacctttgat gctgaagttt acgaagaact ccaagtcgac gctgaaagtc tgatgaaaga
      301 gtacgaaaaa cgtgctaaag atgctggggt agcagatgtt catatcgtca ttgaaatggg
      361 aaatccaaag accctgctag cacgtactat tccagatgcc gaggaagtgg acctcatcct
      421 cgttggcgca actggtctca acgcctttga acgcctcttg gtcggctctt catctgaata
      481 catactccgc catgctaagg tcgatttgct ggttgtgaga gaacaagaaa aaaccttata
      541 atcacaaaga aaaggagccc ctagctcctt tttgtttacg atttatttct ctctttatgg
      601 cgttcgtaag ccttgagctg gcgctgcagt tcctttttaa tagcaggttc tggagcatat
      661 ttttcttccc aattatctgg ttttaagatt ttatgggtca ctggatcaaa atgagccttg
      721 ccatctggaa aaattttccc catattggcc tgatggacaa tatcaaaaat acgttctggg
      781 tccaccccca tcaagacaaa actgccgtag gtgaagtaaa gcgtgtcaat caaggcatcc
      841 acttgcccta tcaaatcttg ctgagcaggt gtcttcttgg ctactttatc tgctgcctta
      901 tcaagggcct gatgaagttg cgatacagct tgaccaaaat cttcttcaga aggactggct
      961 gctcgaacaa actccaccaa ttcttctatt ttaaaaccag ccctatgggt tgcaccctct
     1021 aaatcccaag ctcgaggttc ttcttgggtt cgttcatcca tcatgtggtg gaaggtcttg
     1081 accttattga aatgatagtc acggctgaca aagacttttt ctgaagacaa aacaccaaaa
     1141 tgttccaagg ccttgtggat tccatcttgt tgattgctag tggtaatgtg cttggcagct
     1201 tccttgacac tgctactacc atttcccata gcgaccgaca taccaacgcc agccagcatt
     1261 tcaagatcat tatctgagtc accaaaagcc atgacttggt tgaggtcaaa gccatattct
     1321 ttcccaactc ggcgaatgcc ttctaattta gaatttccct gattgatgac atccgatgca
     1381 aaaggattgc tacgtgtcaa tttcaagtct tcaaaatcag ctgccgcctt ctcagattct
     1441 tctggtgtca tcagcatcaa aacttggtag ataggctgat tcatcaggtg aagcaggtcc
     1501 tcttcctttt ggggaacaac cttgctgacc atgcgattaa aagactgact caccgtccga
     1561 gttaaaacag agggaacgaa gcgactaatt cgttgggaaa aagaacccag accaaaggac
     1621 atgattttag aacccaacat ggcatccttg gtccctagag caatctccgt gccctctttt
     1681 ttagcatagc taattagatg gcgcaaatgt aacttggaaa tagggctcgt gaacaagact
     1741 ctgtctttac taaagatata ctggccatta taggttaccg caaaatccag atccaaatcg
     1801 tccatcaatt ccttaacaaa aaaaggtcct cgccctgtcg ctacgccaac tagtacccct
     1861 tgttctttga caatcttaat cgcatcctta gtggatttca aaacactctt gcgattgttg
     1921 accaaggttc catcgatatc aaaaaaaaca gctttgactt ccatcctatc ccaatctccc
     1981 cttttgtgat acaatgatta taccacattt cagaaagagt gagtaaatca tgcctaagaa
     2041 aatccttgtt ttacatacgg gtggaactat ttccatgcag gccgatgctt ctggcgctgt
     2101 tgtgacgagt tcagataatc ccatgaacca tgtgtccaac ccacttgaag gaatccaagt
     2161 ccacgccttg gactttttta accttccaag tccctatatc aaacccaaac atatgctggt
     2221 cctctaccag aaaattaaag aggaagcaga taactacgat ggagtggtga tcacacacgg
     2281 aaccgatact ttagaggaaa cagcctattt ccttgatacc atggaagttc cccatatgcc
     2341 tatcgttcta acaggagcca tgcgtagctc caatgagctc ggtagtgatg gtgtttataa
     2401 ttacctaagt gctttacgag tggccagcga tgacagggct gctgacaaag gagttttggt
     2461 cgttatgaac gatgaaatcc acgctgccaa gtatgtcacc aaaacacata cgactaatgt
     2521 cagcaccttc cagactccaa cacatggccc ccttggtctc atcatgaaac aggaaatcct
     2581 ctacttcaaa acagctgaac ctcgtgttcg ctttgacctt gatcacatac aaggtttagt
     2641 ccctatcatc tcggcttatg ctggtatgac agatgagctg attgatatgc tggatttaga
     2701 acacttggac ggtttgatta tccaagcctt cggagctggt aatattccca aagaaacggc
     2761 tcaaaaatta gaaagccttc tgcaaaaagg aattccagtc gctctggtat cacgatgctt
     2821 taacggtatt gccgagcctg tttatgcata ccagggtggg ggcgtacagt tgcaaaaagc
     2881 aggcgttttc tttgttaaag aactcaacgc ccaaaaagct cgcttgaaac tcctcatcgc
     2941 cctcaatgcc ggactaacag gacaggcttt gaaagactat atggaaggct aatactcttc
     3001 gaaaatctct gcaaaccacg tcacgtcgcc ttaccgtatg tatggttact gacttcgtca
     3061 gtttcatcta caacctcaaa aacatgtttt gagctgactt cgtcagttct atctacaacc
     3121 tcaaaaacat gttttgagct gacttcgtca gttctatcta caacctcaaa aacatgtttt
     3181 gagctgactt cgttagtttc atctacaacc tcaaaaacat gttttgagct gacttcgtta
     3241 gtttcatcta caacctcaaa aacatgtttt gagctgactt cgtcagttct atctgcaacc
     3301 tcaaagcagt gctttgagct gacttcgtca gttctatcta caacctcaaa acagtgtgtt
     3361 gcgcagcctt taatcagccg cctagtccgc tctatggtat tcattaagtc aacatctctt
     3421 gtttaagagc accaaatcag gaaatcttct cgattccctg attttttcta tttacgtttt
     3481 cgtgttgagc tacgttctgt caaaccatga ggtaagagaa cttcacgttc ttccaactct
     3541 tccttatgca taatcttggt caacatacgc atactaatgg caccaaggtc ataaagaggt
     3601 tgggcaatcg ttgtcaagtt tggacgggta aagcgtgaga tttgtgaatc atcactagta
     3661 ataatttcaa aatcttctgg cacagaaaca cccttatcag ccaaaccgtt caagactcct
     3721 gctgccaact catcacctgt cacaactgct gcagttgcat ttgatgaaat caaacgctct
     3781 gctaaggcgt aaccatcatc atagctatat ttagattcaa ataccaaacc ctcactataa
     3841 gtgattcctg cttttttcaa ggtttccttg tagccaacta aacgaacctt accattgatg
     3901 tcatccacta gcggaccgct aacgaaagca atacgctcat tttctttagc aaggtaactc
     3961 actgcatcaa ttgttgcttg cttatagtca atattgacac ttggcaactg gtgctcaaca
     4021 tcgacagttc ctgcgagaac aatcggagta cgtgaacgcg aaaattctga gcgaatttta
     4081 tctgtcaagt gataccccat atagataatg ccatctacct gctttgaaaa gagggtattg
     4141 acaacagaaa cttctttctc gttatcttca tcgctattag ctaggacaat attgtacttg
     4201 tacatttctg caatatcatc aatcccctta gccaaactcg aaaaataacc attggtaata
     4261 tttggaatca cgacaccgac agtggttgtc tttttacttg caagaccacg cgcaactgca
     4321 tttggacgat aatccaaacg atcaattacc tctagcactt ttttacgggt attctctttt
     4381 acatttttat tgccattgac cacacggctg accgtcgcca tggaaacacc tgcttcacga
     4441 gcgacatcat aaatggttac tgtatcatct gcattcattc cttttcctgt cctttctatc
     4501 tcacacattc ttttacaagt agaggtactg attgaagctc tatatctact tacaaaagtg
     4561 aagatgtgaa aatttcgttt tcatatttct acttattcca ttctatcact aattgtaaac
     4621 actttcaagt gttttttgaa gattgattga aaaaatttca tagaaaacct aggtttagct
     4681 ccttgctacc accttagact aaacaaaaag gaggaaacta agccctccta aagttatagt
     4741 aaaatgaaat aagaacagga taaatcgatc aggacagtca aatcgatttc taacaatgtt
     4801 ttagaagtag aggtgtacta ttctagtttc aatctactat aggtattgtt ccattcacta
     4861 ccgtcaattt tagcacatag tcttcatgaa aatattatat catcataacc aaccagattc
     4921 tttcgcgata ttagctgcct ctgttcgatt acctgcatct agtttcgaaa gaatattggt
     4981 gacatagttt cggactgttc cgttggatag ataaagtttg tctgcaattt cttggttaga
     5041 gaagccctga gcaattccct ttaaaactgc gatttcttgc tccgttaatg gattgggatg
     5101 catcatcacc acttccatca attcaggcga atactccttg cgtccttcga ggacggtgtg
     5161 caaggtttgc atgaggtctg caatgtttct ttcttttaat acataagcat ctactccagc
     5221 cttgaccgca cgttcaaaat acccaggacg cttgaaggtc gtcaccacaa ccacctttgt
     5281 ttcaagcttt tctgctcgta tccactccaa gacttcaaga cctgtcttaa caggcatttc
     5341 tacgtcaagg atggcgatat ctacagactc cttttctaat agttggattg cttcttgccc
     5401 attcttggct tgaaagacag actctacatc cggttgaagc atgagcaact ggcacatggc
     5461 atctcgcaac atactttgat cttctgcgac taatactttc atctactttc tctccttata
     5521 aagtagtcga acctgcactt cagttggatg tttctgactg attacactta cttctcctga
     5581 aaatggaaaa acacgatttc ggactgtatg gagctcatcc ccgcttatag aggcaaagcc
     5641 acagccatca tctctcactg ttagaatgag ttctttctct gtccgttcta atttcaagta
     5701 gactttagac gctttagcat gtttgatgat attggtcact aattcaagca aaatcatgga
     5761 agccgttgac tccaattcct gagttaagct agacttgtcc aagtgattct caacttgaac
     5821 ctcaattcca gcaatttcta acatcttttt cacagtctct agttcggatg tcaaagttct
     5881 agacttaaga ttttccacaa tggttcgcac ttcattcatg gaatccttgc tgatctggtg
     5941 aatttctttt aattcctttt ccacctgtgg ataagcctcc atctgaaata actgcaaggc
     6001 taaatctgtc ttgacactca gcatagcaaa ggtatgtccc agactatcat gcaaatcctg
     6061 accgatacga ctacgttcat tttcagcaag caatagattt atctgagcat tttgcttgac
     6121 ctgagcttct ttcaaatcct cgacaatacg aatccgaacc aatccaaaag tcattaaatc
     6181 gacaaaagta agaattacaa gtagatagaa tagaaactca acttcgattc tctgaaaaat
     6241 caacagttgc cccacaacaa ggacttgagc aagaagaaaa gtccagacat gtaaagactt
     6301 taaactacgt acgctgaaat gataacttaa gagattggat aggaaaaaga aaaaccagat
     6361 ataattaaca gcaacaaagg cagtattccc aactacataa gtcagcatga ggccccaata
     6421 tagccaagat aggcgctggc tcttagttgt taaaacaccc aaatatgcca ctacaaatag
     6481 aatatcaatc aataaatgcc aggcagaaag ccacccagtc actacagaca ggatggggaa
     6541 aatcataaaa attaaactga tccaaaacat ataatgtatt cttttcagtc tttcaagcat
     6601 taagcattct ccttatgacc ttgaaggtaa atagtcaaac caaataaaac tgctgaaaaa
     6661 acaagtagat aaactgtggc tgatagattg attccaccct catttaagaa ggtcttgatt
     6721 aattccatca actgataact tggtaaacgc ttcccgattg cttgcatcca gtctggaaat
     6781 aaagagacgg gcatccagag tccgcctaaa acagccaatc ctagataaag aagattgccc
     6841 acgacagaca tcagctgact agttggtaac agcgtcaagg ttaagccaag tgctacaaaa
     6901 gcaatacttc ccaaaatcag caaaatcgca gctccaaccc attttactag aggcatgtct
     6961 acacctctta caaaatgtcc aactgtaaaa acaaccagaa ttgagaccaa ataatcaacc
     7021 agcatacttg ttatctttga tagataatat tctaccatat ttactgggct atgacgtaat
     7081 attttctgcc agttgttcat cttgtcggta tgtaagacag ctgggaatga aaagatagcg
     7141 gtcgacatca tggaaaagcc agtcatggag atgaggtaat cacgcataaa attcgcgggt
     7201 ccacctggtg tgtcctggta catgccagaa aagaatagat agaaggctgt cggcatccct
     7261 actgacaata gataatagat caattgtcgt ttggtcaata aaaattctat cttactaagt
     7321 gctagccatc gtttcatctt agttatctcc cttctgcgtt tcttcaaaga ttgtatccaa
     7381 caaactacga ttattaactt caatttcttg tatgccacat cctgcttgaa ctaacagttc
     7441 ccaaaaagca tctgcttcgc gtgtgactac ttgtagagca tcctgttttt gtgaccagtt
     7501 ttcaaccaag ttagactgct caatgacttc cttgtatgcc agaggaagga taaaatgctt
     7561 ttcaattccc tcactacgca tagctagagg cgtcgtatca cgaatcaact ctcccttatt
     7621 taagagcaaa atgcggtctg cagtgtgttc cacttcctca atataatggg acgaatagag
     7681 aatggtgact ccttgcgctt ttaactcctg gacaatttcc caaaaacgtt gacgagttga
     7741 ggtatccatg gacgcagtag gctcatctaa aaaaacaatc tttggtcgcc caattaaggt
     7801 caagacgaaa gaaaagagac gcttttgacc gcctgataat ttttctgcca actgttcttt
     7861 ttgttgcttg acaaactgca atagttgatc aacttcctga ttgctcaaag gatttggata
     7921 gatacgttga aagaaagcaa tcaactcttt aacttttaat ttctgaacga ttacattttc
     7981 ttgagggagg taagccctag tatagtctaa cttagaactc gtcactggta aaccttgaat
     8041 agatacttga ccgcttgtga ccagtttatc tccaagcaaa caatctaaga gtgttgtctt
     8101 tccggcacca tttggaccaa ttaaggcaac acattcacca tcgttgattt caaaggagat
     8161 gttccgcaaa atttccttgt cttttatttt cttactcaat ttttcaactt taaccacagt
     8221 catgagactc tcctttcaac cactccattc ccatagggaa aacgacgaaa atcataaatc
     8281 caaaccccaa agcaccacga atgaattggc gaagcaaggc ttgatcaaac caatcagtaa
     8341 acatttccac taaccatacc aagagtgaca ggccgataaa gaaatagatg atccctttct
     8401 tcattcctca aactcctttt tcacatctct gactaatttc aaaccttctc taacaagcca
     8461 agacatgatt ccaaagccag caaagatctt tcaaggaaaa tggtagaaat cctcatccaa
     8521 tcccgaaaac atgagatagg tcataactcc tgctactact aaacttactg cgataatcat
     8581 tttatttctc atctcttctt cccccatttc atactacaat tatagtcttt taaaatcaga
     8641 ggaggcagaa gcttctgtca ctagaaaata tgacaaatgt cataaaaaat tctgttcaaa
     8701 acaagcaaga tacactatac aataaaacac aatcagaaaa atctaaggca acttcctcaa
     8761 aagagatatc aaacccaatt cacaccataa tgtaaactaa tacttattta aaatcaaaaa
     8821 gagtagaaat ttttatcag
//
LOCUS       DAVKGU010000082         8640 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKGU010000082 DAVKGU010000000
VERSION     DAVKGU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.083"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            1..320
                     /locus_tag="VW077_001718"
                     /pseudo
     CDS             1..320
                     /locus_tag="VW077_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(445..648)
                     /locus_tag="VW077_001719"
     CDS             complement(445..648)
                     /locus_tag="VW077_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455702.1"
                     /translation="MIKPYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(641..1723)
                     /locus_tag="VW077_001720"
     CDS             complement(641..1723)
                     /locus_tag="VW077_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9455703.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1849..2631)
                     /locus_tag="VW077_001721"
     CDS             complement(1849..2631)
                     /locus_tag="VW077_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9455704.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2631..3974)
                     /locus_tag="VW077_001722"
     CDS             complement(2631..3974)
                     /locus_tag="VW077_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9455705.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4073..5389)
                     /locus_tag="VW077_001723"
     CDS             complement(4073..5389)
                     /locus_tag="VW077_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9455706.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVTAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5582..6808
                     /locus_tag="VW077_001724"
     CDS             5582..6808
                     /locus_tag="VW077_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9455707.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKALT"
     gene            7007..8581
                     /locus_tag="VW077_001725"
     CDS             7007..8581
                     /locus_tag="VW077_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9455708.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2466 a         1681 c         1930 g         2563 t
ORIGIN      
        1 gacctactac tatcacttga aacagctaga caaaacagat aaggaccaag gctggcaata
       61 tcaacacgat tcttatcatc ggtttctaga gagtaaggga attcaagcat ccatgtcacg
      121 taagggcaac agcccagaca acggtatgat ggagtccttc tttgggattc tgaaatcgga
      181 aatgttttaa gtcgtttcag tcgcttaagc aattggaaca agctattgta gactatattg
      241 attactacaa caacaaacga attaaggtaa aactaaaagg acttagtcct gtgcagtaca
      301 gaactaaatc cttcggataa attaattgtc taactgtttg gggtcagtac acaatacctg
      361 atagtatgcg ttttattgtg ggaagatttt cctcattttc tagtgaaatt gaactttttc
      421 taacatcttt tgatagatga cttcttaatc aattatcatt tccattgttg tttcttgggg
      481 tttcatccct tgtctttgat aaaagcggag agcaccctcg ttatcattcc agacacttag
      541 ggttagatta ttgcagccaa tttctttagc ataatagcag aaaaatgaca ataaaatagc
      601 ccctagatat tttgaaagga aagcataggg gctatatggt ttaatcaaaa taaagcaaat
      661 ctttgctggt gcttgtaaag aggtcgaagc catccttggt aacaacaccg cagtcttcaa
      721 tacgaacacc gactttacca gggatataga tacctggttc aacagagaag cacatgcctt
      781 cttcgatgac catgtcgttt ccttccatga tagatgggaa ttcatggaca tccataccga
      841 taccatgccc gagacggtgg ttgaagtact caccataacc agctttttcg atgacctcac
      901 gggcagcgcg gtccacttca tgagcagtca cacctggctt gataaagtca agagcagctt
      961 gttgggcttc aagagtcaag ttgtaaatat ctttcttgaa ttggtctggt ttgccgacag
     1021 cgactgtacg agtcatatct gacgcatagc cattgaccag aacacccagg tcaaagagga
     1081 gaagagcatc attttcaacc ttattagctg ctggaatgcc gtgtggattc gcagcattat
     1141 caccagtcaa gaccatggta tcaaagctca tttcgtaacc ttcacgtttc atggcaaagt
     1201 caatttgtgc gatgatatct gtctcagtct tatcaagaga aatattatca aaaccaacat
     1261 gaacagcctt gtcagcataa agacctgcaa ccatcatttt ttgcacttca tcagctgatt
     1321 tgatgaggcg catgcgttgg atacgaggag tgaggttgtc aaactcagca gtctcaaaaa
     1381 ctgttttcaa accatggtat ttggtcaaga tgagattgtc aaactcaaca gcgacacgtt
     1441 tgaagtcaag ttgtggaaga gcatgtttga ttttttgcca tggattttca gaatcgacat
     1501 agcccactac tgggaaggaa acggtgctac ttgcacgttc tacttcaaga gctgggacaa
     1561 agaggagagg ttcctgatcc gctaggacaa agaggaacat ttggcgttca tggggatcac
     1621 tgtaaaaacc agtgaggtaa ttgattgtga cggggtcaga tacgacagcg acgtctagtt
     1681 tttctgattc aagatatgtt aggatttgtt gtaatttaga catatgctac cttctttcta
     1741 cccctctatt ttggcaaaaa atgagagaaa atgcaaggga gaagggtaaa agaaaagaca
     1801 aaaaactccg acaagataac ggagttcttt gatatcattt tcctttgttt aagaaaagtc
     1861 agccttgctt ttgacgtcac tgtactcttc agcgatttct tggctgagga tatcctcata
     1921 ggcagggagt tggatatcct gaccatattt cttcttgagg gcagtagtaa ctaggcgata
     1981 agccagattg gcattacgag agaggatagg tccgtggaag taggaaccaa agacattctt
     2041 ataatgaacc ccttcaccga ccttttcttc gttgtttcca tttccataga caacctgtcc
     2101 cagcggtttt tggtcatcag agaggaaggt acggccttgg tgattttcaa atccatagta
     2161 ggtttcatcg aaatcttcat tgtgaatctt gatgtcaccg ataaaacggt tattggtctg
     2221 gttgagcgtg tagtgtccca tgacccctag cccttcgata cgttttcctg aagcttcaac
     2281 ataatattga cccaatagtt ggaaaccacc gcagatagcc agaactacac cgtcgttttg
     2341 gatgtagttg tcaatgctct cttttttagc aggtaggtcg tctgcaatga tactttgttc
     2401 aaagtcttga ccaccaccga aaaaggcgat gtcgtagtga ttttcatcaa agtcatcatg
     2461 gagagaaacg atgtcaacgg tcacatgggc tcccagtttt tcagccacat acttgagcat
     2521 gaggatgttt ccattgtccc cgtaggtatt catgagattt ccgtagaggt gggcaatgtt
     2581 gagctgatag gggtaattgc catcttttga ggaaagtgaa gtataaacca ttagttcatc
     2641 tcctttctaa caatctgacg actagccagc agttcacgaa attccagcat ggcagtataa
     2701 gttgccagaa tataggcatg cttgcagtct tgattctcaa tggtcttgag aacttgctcc
     2761 agattactcg tttcagtgat tttctcagct ggatagccag tcactcggag gcgacgagcg
     2821 atttcagaat gacgaacacc gccagcgttg atttcaggaa tgtccatgtc agtgatttgt
     2881 tcaaagtctg catcccagat ccagctagtg tcaattccat ctgcatagtt ggcattaagg
     2941 aggacagata ggctaaatgg ataaggtgct agtttgatca tttcgatagc ttgggttgca
     3001 ccgactggat ttttaatcaa gacaagggta cattccttgt caccgatatg aaaggtttct
     3061 tggcgtccaa agacagcacg gctcttgtca aatccctgtt tgatgagttg cgaatcggca
     3121 cctaggaaac gggcgatggc cacagcagct agggcgttat agatattata gagcccgccg
     3181 atttggatac cgtattcttg gccgtctatg acaaagcgag agcgattgtt ggtcaactca
     3241 accagttttg tcaaacgata gtcgagatca ggacgtttgc atccacaacc ttcacagata
     3301 taggcaccca agtttgcata ggtattatgc tcatatttga ggatgccttg gcagtcagga
     3361 cagagaatcc cttcggtatt gtagtgagcc agttgggctg gtcctttttc caagtcaaaa
     3421 ccaaaatact ctatagggtt tggaatagtt ggcttgtaga aaagtggact gtctccgtta
     3481 aggagaacag tagcagttgg aactttccga atggcatcca atatcatgtt ataggtagta
     3541 tagatttcac cgaaacggtc catctggtca cggaagatat tagtaatgac aaaaagacta
     3601 ggctggatat agtcacagat acgagataga ctggcttcgt caatttcgag gacggcaata
     3661 tttttcccag tttttgaaga tttggctgtt aggaaggttg ttgcaatccc tgtaatcatg
     3721 ttggcaccgc ttgggttggt tagaacttga ccataaacct cttttaaaat gccgacagtg
     3781 agggcagttg tcagggtttt tccatttgtt ccagtgacaa cgacaatctc gtagttctta
     3841 gctaggtttt gtaaaatatc tttatcaaat tgaagggcga ctttacctgg gagcgtactt
     3901 ccacggccaa gacggcttaa aatgaagtgg gaagaacgcc cagcaagaag gcccaaagta
     3961 gtttttaagt tcatgtttct attatatcat aaaagaggag aaagacagat ttgagattcc
     4021 gcagaagtaa aaacagccca aagagggctg ttcgatacga ttaaatctca acttaaatcg
     4081 caaacaagtc attcaagttc tcagccaagg ttgggtgagt gaagatttgt tttgtgaagt
     4141 aagtgtaagg aatcttgttg tccatagcaa cagtgatgat gttgatgatt tcttgagaac
     4201 cttctgagaa gatgcttgct ccaagaattt cttttgtttc agtattgaca acagctttga
     4261 aggcaccgcg aaggtctcca tttacgtgac cacgaggcat tgctgtaacg gggatttcct
     4321 taacagcgta tggaagtttc aaatcagctg cttggctttc agtcaaacca acttgtgaaa
     4381 gtgcaggtgt gatgaacata gtatttggca cattgagacg gtcttcaagt gtgtagctgc
     4441 catctccagc aaggtagctg taaacaacac ggaagtcatc aagtgaaatg taagtaaatt
     4501 gaaggccacc gttgacatct ccaactgcaa agacaccagg aacgtttgtt tgacagtgtt
     4561 tgtctacttt aatagcacca cgttcagtta gttcaatatc tgtattttca agttgaagtg
     4621 gttctacatt tggtttgcgt ccagttgcgt agagaagggc gtcgaaacgg taagtttcgt
     4681 cttcagttac gacaagcact tggtcaccat cgtttttgat ttcagtagta tggatatttt
     4741 gaagcaattc aatgccatct tcttccatgt attgtttagc aagagctgcg atggaaggtt
     4801 ctgcacgagg taggaatgta tccaaggcat ctaggactgt gaccttgctt ccaagtttgt
     4861 tgtaaaggcc ggcaaattca agaccgatat ttccgccacc aaggattcca agtttttcag
     4921 gtaatttgtc caagctttgg atacctgttg agtcaaagat gtttttgctt gtagcaagtc
     4981 cagggattgg caagacgttt gaaacagcac cagtgttgat gacgattgtt tcagcagtca
     5041 gttctttctt ttcatcacca gcttggattt cgatgacttt atttgaaagg aagtgagctt
     5101 ccgcatcaaa gatatctacg cctgtaccag caacagtcgc atagttttta ccgttgaggc
     5161 gaccagtgat cgtgttttta gtagcaatga cttcttcaaa agacaagtcc ttttcagcag
     5221 caactagcaa ggttttagtt gggatacaac caatgttgat acaagttcca ccgtacatag
     5281 ccttgctgcg ttcaacgagg gcaacttttt tgccagctga agccaattta cctgctagtg
     5341 ttttaccagc tttaccaaat ccaataacaa ttaaatcgta tgttaacatt tatcttcctc
     5401 ctattcgaat ttcattctag cacaagaaaa aaacttttgc acaattctgc tacggaaaag
     5461 acagaaaaag ataaaaacgc tttccagaca aaaaactagt aattttttga caagcttgat
     5521 aaactaaaat cctatctatt tttatataag atagaataaa tgttcgaaaa taggaggttt
     5581 tatgaaatcg aatcgttata ttattgcctt tgctggggtc attttacact taatgctggg
     5641 ttcgacttat gcttggagtg tttatcgtaa ccctattatt gaaaaaacgg gatgggatca
     5701 ggcttctgtt gccttcgcct ttagtctagc aatcttttgt ttgggcttat cggctgcatt
     5761 tatggggcgt ttggtagaaa aatttggtcc gaaagtcatg ggaagtctat ctgcttttct
     5821 atacgcaggt ggaaatatct taacaggatt tgcaatagac cgtcaggagc tgtggttgtt
     5881 gtatctcgct tatggcattt taggtgggct tggtttggga gcaggctata ttacccctgt
     5941 gtcgacgatt ataaaatggt ttcctgataa acgtggtctc gcaacaggtt tagcgattat
     6001 ggggtttggt tttgcttctt tattgactag tcccatagcg caacacctca tcgcaggggt
     6061 agggcttgta gaaacttttt atattttagg agcaagttac tttattatca tgctcctagc
     6121 ttcacaattc attaagcgtc caaatgagca agagcttgca attttatctt cttcagggaa
     6181 agaaaaaaca gcctctttga cgcaaggaat ggctgcaaat caggctctaa aaagcaatcg
     6241 gttttatatg ctttggatta ttttctttat caacatagct tgtggtttag gcttaatttc
     6301 agcggcatcg ccaatggcac aggagatggc tggcttgtct acaagtcatg cagcagtaat
     6361 ggtgggtgtt ttggggattt tcaatggatt tggtcgcttg ctctgggcga gtttgtctga
     6421 ctatatcggt cgccctctaa cctttagtat attactgctt gtcaatcttt tcttttctct
     6481 ctcactttgg ctctttacag attccgtttt atttgtagtt gctatgtcta ttttgatgac
     6541 ttgctatgga gctggttttt ctttgattcc agcttatctc agtgatattt ttggaaccaa
     6601 ggaattggcc gctctgcatg gttatatttt aacagcttgg gcaatggctg gtttagcggg
     6661 acctatttta ttagcagaga cttataaaat ggctcattcg tacacacaaa ccttgttcgt
     6721 ttttctcatt ttatacagta tcgccttggc tttgtcttat tatctaggtc gttcaatcaa
     6781 aaaagaaagt caaaaagcgc ttacatgatt ttctgtagaa atggggctat cttttgctct
     6841 ggtcttgtgt tatactagat aggttgcaaa gaaaacagta cttttctttt gtggaaaaga
     6901 agcaacacga ttttatatct agttatatga aaatacgtca taaaaagaaa agtataaact
     6961 aagccctata gggtggcttc gcaccacctt tagaaagaag aataacgtga aatttaatga
     7021 attaaacttg tctgctgatt tgctagcaga aattgaaaaa gctggttttg tagaagctag
     7081 tcctatccaa gaacaaacta ttcctttggc ccttgaaggc aaggatgtta tcggtcaagc
     7141 tcagactggt acaggaaaaa ctgcagcctt tggcttgcct acccttgaaa aaatccgtac
     7201 agaagaagcg actatccaag ccttggtcat cgctccaact cgtgaactag ctgtccaaag
     7261 tcaagaagaa ctcttccgct ttggtcgtag taagggagtc aaagtccgtt cagtatatgg
     7321 cggatcaagc attgaaaaac aaattaaggc tcttaaatct ggtgcccata ttgtggtggg
     7381 aactccaggt cgcctcttgg acttgattaa acgcaaggcc ttgaaattac aagacattga
     7441 aacccttatc cttgacgaag cggatgaaat gcttaacatg ggcttccttg aagacatcga
     7501 agccattatt tcccgtgtac ctgagaaccg tcaaactttg cttttctcag caactatgcc
     7561 agatgccatc aaacgtatcg gtgttcagtt tatgaaagcc cctgaacatg tcaagattgc
     7621 ggctaaggaa ttgacaacag aattggttga ccagtactat atccgtgtta aggaacaaga
     7681 aaaatttgac accatgactc gtctcatgga tgtggcacaa ccagaactcg ctattgtatt
     7741 tggtcgtacc aaacgccgtg tggatgaatt gactcgtggt ttgaaaattc gtggcttccg
     7801 tgcagaagga attcatggcg acctagacca aaacaaacgt cttcgtgtcc ttcgtgactt
     7861 taaaaatggc aatcttgatg ttttggttgc gacagacgtt gcagcgcgtg gtttggatat
     7921 ttcaggtgtg acccatgtct acaactacga tattccacaa gatcctgaga gttatgttca
     7981 ccgtatcggt cgtacaggtc gtgctggtaa gtcaggtcaa tctattactt ttgttgctcc
     8041 aaacgaaatg ggttaccttc aaatcattga aaacttgact aagaaacgca tgaaaggtct
     8101 caaacctgca agtgtagaag aatccttcca atcaaaaaaa caggtagctc tcaagaaaat
     8161 cgaacgtgat tttgcagatg aaaccattcg tgccaacttt gagaaatttg gtaaggatgc
     8221 tcgcaaattg gctgctgagt ttactccaga agaattggca atgtatatct tgagtctgac
     8281 agtccaagac ccagatagcc ttccagaagt ggagattgca cgtgaaaaac cactaccgtt
     8341 taaaccatca ggtaatggtt tcggtggtaa agctaaggga ggtcgtggag gccgtcgtgg
     8401 ggacgaccgt cgagagcgtg atcgccgtgg caatggtcgc cgtgatgagt tcaaaaaagg
     8461 aagtcgtggc aacgatcgtt ttgataagga aaaacgttac cgtaaggata ataaaaaacc
     8521 acgcaatact ttaagcgaaa agcaaacagg ctttgttatt cgtaacaaag gcgataaata
     8581 agaaaaagcg gttcaaaatg aatcgctttt ttatataagg agaccgaatg tgaaaagaca
//
LOCUS       DAVKGU010000083         8134 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKGU010000083 DAVKGU010000000
VERSION     DAVKGU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.084"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(147..497)
                     /gene="rbfA"
                     /locus_tag="VW077_001726"
     CDS             complement(147..497)
                     /gene="rbfA"
                     /locus_tag="VW077_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9455709.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(748..3540)
                     /gene="infB"
                     /locus_tag="VW077_001727"
     CDS             complement(748..3540)
                     /gene="infB"
                     /locus_tag="VW077_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9455710.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(3557..3856)
                     /locus_tag="VW077_001728"
     CDS             complement(3557..3856)
                     /locus_tag="VW077_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9455711.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(3849..4142)
                     /locus_tag="VW077_001729"
     CDS             complement(3849..4142)
                     /locus_tag="VW077_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9455712.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(4164..5300)
                     /gene="nusA"
                     /locus_tag="VW077_001730"
     CDS             complement(4164..5300)
                     /gene="nusA"
                     /locus_tag="VW077_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9455713.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(5344..5823)
                     /gene="rimP"
                     /locus_tag="VW077_001731"
     CDS             complement(5344..5823)
                     /gene="rimP"
                     /locus_tag="VW077_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9455714.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(5949..6584)
                     /gene="trmB"
                     /locus_tag="VW077_001732"
     CDS             complement(5949..6584)
                     /gene="trmB"
                     /locus_tag="VW077_001732"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9455715.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(6581..7375)
                     /locus_tag="VW077_001733"
     CDS             complement(6581..7375)
                     /locus_tag="VW077_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9455716.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(7536..8033)
                     /locus_tag="VW077_001734"
     CDS             complement(7536..8033)
                     /locus_tag="VW077_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9455717.1"
                     /translation="MILVSLLVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVL
                     ETLVYIHFFAVSSGSGVQHLAEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDN
                     SWLGLVLTSSLFSFMHGPSNVPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWP
                     LLYYL"
BASE COUNT         2121 a         1915 c         1549 g         2549 t
ORIGIN      
        1 tcgatcagga cagtcaaatc gatttctaac aatattttag aagtagaggc gtactattct
       61 agtttcaatc tattataaaa gaatattttt ccatttcaaa ttcaacctat tttcctccac
      121 caaaaaagag gggcaacccc tcttctttag ttcttatcca gattgcgtag catctcgtca
      181 atcttgtttc catactcgat ggactcgtct ttgacgaagg tcaaatctgg gattttgtac
      241 aatttcaaat tgcgaccaag ttcacgtttg atggtaccag ttgctttttc aagcccgatt
      301 tgggcttttt ggttatccga agcaaggtta ctcaaaatgg tgtaataaac cttggcaaca
      361 gacaagtcac ccagcatctg aacatctgtg atggtcacac cttggacacg tggatcacgg
      421 actttctttt gcaaaatctc attgacttca cgcttgattt ccatgcccac acgatctgta
      481 cggaaatgat ttgccatgat attcctttct ctactttgaa ccaaaagcta ggccagcaga
      541 cctagctatt ttaaacttct tgattttcag tttaaattga aacgaagttc aatttgaagt
      601 catctgcttc tttcgccgtg gtgaaagtga tggaactgat ttatcagtcc catcacgggg
      661 gatttttgag accctaggct caaaaataag caatgaaacc atcggtttgc ttgcgtccct
      721 caccacctaa gaaaggagca aaaaatctta tctcttgatt tcttccatga catacgcctc
      781 aatcacatca tccatcttaa tatcattgta gccgtcgatc atcaatccac cttcacggcc
      841 gtttgtaact tccttaacgt cgtctttata gtgtttcaag cttgcgagtt caccatcata
      901 gataacgaca ccatcacgga taacacggac tttagagtca cgggcaacct taccgttgat
      961 aaccataaat ccaccgatag ttcccacttt agacaccttg aaggtttcac ggataaccgc
     1021 ttcaccaata actttttctt caaattctgg atcaagcatc cctttcatag cttcttccat
     1081 ctcttcgata accttgtaga taatgctgtg aagacggatt tccacatcgt cagcttctgc
     1141 ttgttgacga gcttgtggtg tagggcgtac gttgaaacca acgataaagg catttgaagc
     1201 ttcggcaagg gtcacgtctg attcgttgat agcaccgacc gctgagtgga cgatagtcac
     1261 tttgacacct tccacgtcaa tcttttgaag tgaggcagaa agggcttcaa cagaaccttg
     1321 tacatcagcc ttgatgataa cattaacaga tttgagttcc ccagctttaa gggtatcaaa
     1381 gaggttttca aggctaacac gttgggtagc ttgacgttgt ttcatgaggg cacgtttggc
     1441 acgctcttca cctgctgcac gcgcagattt ttcatcctcg taaacggcaa agtggtcacc
     1501 cgccatcggt gcttcgttca aacctgtgat agagactggt gttgatggtc cagcaacttt
     1561 aacacgacga ccaaggtcgt tggtcatagc acggacacga ccgaaggtat ttccgacaac
     1621 gattgggtct tgaacattca aggtaccttg ttgtacaaga agggttgcga ccgcaccttt
     1681 tcctttatcc aagcgcgctt cgataaccgt accgattgca cgcactgttg ggtctgcctt
     1741 gagttcttgg atttcagcta caagaaggac tgtttccaac aattcttcga tattttggtt
     1801 gaatttagct gagatttcaa caaattcaga atctccaccc caagcagttg acataactcc
     1861 atgctctgcc aattcaccga taacgcgttc tgggttagca cctggtttat caatcttgtt
     1921 aatggctacg atgattggaa cgttggccgc ttttgagtgg ttaatggctt cgatagtctg
     1981 aggcataacc ccgtcatctg ccgctacgac caagatcgta atatcggtaa cagaagcacc
     2041 acgcgcacgc attgatgtaa aggccgcgtg tcctggtgta tcaaggaagg taatcttctt
     2101 accattttcc acgatttggt aggcaccgat atgctgagta ataccacctg cttcacctgt
     2161 cgcaacacgt gagttacgaa gagtatccaa aagggttgtt ttaccgtggt caacgtgtcc
     2221 catgatagta acaactggtg gacgctcaac caattcatct tcattgagat aaccatcttc
     2281 gacaaagaaa cgttcgatgt cagcattatc cacttcaacc ttttgtttgg cttcgatacc
     2341 gtaatccacc atgaggagtt caattgtttc cccatccaag gattggtttt gtgtggccat
     2401 gacacccatc atgaaaagtt tcttaacaat ttcagctggt tcacgtttga tacgttttgc
     2461 gatttccgca acggtcatac catctgtata ttcaaattct gttggcaatt catggaattt
     2521 acgctccgta acaggttttg gagtctgatt acggttgttc ttgttattgc cttttttgtt
     2581 ctttttgttg ttattccagt tactattctt ttgatttctc acttgatttt gactacttcg
     2641 attcttttgt tgttttctag gaccatcttc ttcatgatca taatcgtcac gatttttgtc
     2701 tggtcgagct tgttttttac gacgtgtatc cactgcaggt tcttttttct cagggacgac
     2761 ttcaaccact gcttgaactt gctgtgcttg ttcagctaac ttagccgctt cttcaaagat
     2821 ttcctctggt tccttgcgtt tgttagcttg agccaaggct tctttagcag cctgatactg
     2881 cttgaagcgt tcctcacttg aacgagcgta ctctgcattt tgctctgctt ttagggctgc
     2941 tgcacgggct ttaaagtcaa tacgtggagc cgcttgattt gaacgtttat cctcttgctg
     3001 acggcgctca tttctacgtt tttgctgacc ttgctgcttc ttagcttggt cattaaagcg
     3061 acgattgtcg cggttgcttt gaccttgttt tccaccatta cggccgtcgt ttttctgacg
     3121 gtttccgttt tgttgttggt cacggttatt gcccttattt tgcttgcgtc gttctgcctg
     3181 ctctttagca cgggcttcac gctcagcctt gaaattacga ctttgcggtt tcactgctac
     3241 tttttctgtt ttcggagcaa ctggctcagc cggttttgcc tcttccttag ctacagctgg
     3301 tttagctggc tcagattttt cggctttctt ttctgcactt acttttggtg ctgcaggttt
     3361 tgcttctact ttcggagcag ctgcaggctt aaagctggca gcaatttttg cagcgacagc
     3421 ttcttccaca cttgatgagt ggcttttcac atccaagccc aactcttttg cacgcgctac
     3481 aacttcttta ctttcttttc caagttcttt tgcgatttcg tacaatcttt tcttagacaa
     3541 atcatgtcct cctcttctat tccataagag acctcatttt ctttgtaaat ccagcatctg
     3601 ttacagccaa aacctttctc gatttcccga ctgctatgct taattccagt gttgaaaaca
     3661 cggttacaat ttctacttga taataatgac ttttatcttg aatcttcttg gtcagattgg
     3721 gtccagcatc atgagctaga aagaccaact tggccttgcc gtcttgaatg gccttgacca
     3781 ccaattcttc acccgatatg atgcgccctg ctcgctgagc aagccccaag agattactta
     3841 tcttttgctt attcaagtcc caactctctt cttttcactt tgtgatccac ataagcgatc
     3901 aactcgtcat aaaagctttc ttccacttcc atgctaaagc tgcggttaaa gaccttcttc
     3961 tttttcgcct ctagggcttc tgcattgtct agtttgatat aagcgccgcg gccattggcc
     4021 ttgcccgtag gatcaataaa gacttgtcct tccttgttct tgacaatgcg gagcaaatca
     4081 cgcttatcaa tcacttcgtt agacacaaca gacttgcgca aagggatttt tcttgttttc
     4141 atctttccct cctctagcag cttttattct tctacagtat cgttttctac ttccaactct
     4201 actgaagcag cgtcttccat ggcttcaaat tcgctagcag acttgatatc gatacggtaa
     4261 ccagtcaagt gagccgccaa gcgcacgttt tgtccacgac gaccaatggc aagagaaagc
     4321 ttgttatctg gaacaaccac caaggcacgt ttgctgtcgt tttcatcaaa gataacttgg
     4381 tcaacctcag caggagcgat ggcattgtag ataaattcag ctggatctgc tacccactcg
     4441 ataacatcga tattttcttc gattggtacc atgcggtcat ttttagcatc gtaacgagct
     4501 gggtggaatt tgctagtaat cttcttgata ttagcaccac cacgtccaac gattgtaccg
     4561 atagcatcca cgtttggatt gtggctacga acagcaacct tcgtacggtc acctgcttca
     4621 cgagccacgc tcatgatttc aacagttcca tcataaactt ctggaatttc ttgctccatt
     4681 aaacgtttga tcatttctgg atgactacgg ctaacaaaga cgttcacacc acgagggttg
     4741 tcttcaacct tgtaaacata aacttcgata cgatcatgag aagcaaaaac ttctccagga
     4801 atttggtctt gttttgacaa ttgggcttcg atgctaccaa ggttgacata gataaagcgg
     4861 ttgtcaaagc gttctactgt accagacatg atttcttgct catgttcttt gtaagtattg
     4921 taagtgatgg cacgtgtttg cttgcgcatt ttttccatga tggtttgttt ggcagactgg
     4981 gctgctacac gaccaaactc agctggtgct tcttcaaact tgattttgtc tccaagttca
     5041 taagctgaat taatggcaag agcatctttc aagctgattt ccaaacggct atcaaatact
     5101 tcatcaacaa cttcacggac agtataaact gtaaagtcac ctgttttttc gttgaagtca
     5161 atagctacgc tgtctgactg accatagcgt ctgcgataag cggaacgaag cgactctact
     5221 actgcgtcga tgatatcttc ttttttgatt cccttgtctt cttccaaaat gcggaaggcc
     5281 tctagcattt ctttactcat gttttcttca cttttgaatc ctcaaaagct atcctttctt
     5341 tttctataat ttaactgcta aacgtgcttt tgatactaaa ctgtatggaa tttggacggt
     5401 tttcttacgc gtcttgtcca tatattccat agtcaactcg tcctcttcga aggccaacaa
     5461 ggttccttca aagacctttt gcttatcgat ggcttggtag agcccgacat ggatgtattt
     5521 tccaaccgct ccagcgacgg catccttggt tttcaaaggg cgttccaaac ctggactggt
     5581 aatttctagg aaatattgtt ctgggaaggg atctggcttg atggtgtcta ggacaggact
     5641 gataatttct gtcaagtctg ccgtgtcgtt caaggtaatt ccttcgggtt tatctacaaa
     5701 aatactgaga atcatgtcac tgccaatctt tccatactcg atatccacga gttcaaaagg
     5761 agcttctatg acaggttcta caacttctct gactaattct acgattgttg cgattgcgtc
     5821 cacctcctca taagcaagag gcgaagatat ttccccgcct cacttttcat attcttacta
     5881 tcagtatagc acgtttgcca acttatgtaa agcaaaagca cttgtacagc ctaattttag
     5941 gctatctctt aaaattctgc ctcaactcgg tagataactt gccccttgtt tgagaatttt
     6001 tgctcgtatt ctgtcatgac attgccttca aaatcactgg catgcaaatc taaccagaca
     6061 ccattgagtt tcatgccata ttgagaaaag ctcactaaac tgtactcaaa caagccacgg
     6121 ttatccgtct tgaaatgaat ttctccattt tcaggcaaga tacgtttgaa ggtatccaag
     6181 aaggtcttgt aggtcaaacg acgcttttca tggcgttttt tcggccatgg atctgaaaag
     6241 ttcagataca agcgatcaat ctcaccgtct tcaaaatagt cagttaagtc agaaccatct
     6301 acccacaaga gcttgatgtt aggcactcca acttcaagca ccttgtccaa agcgtagctc
     6361 aaaacagact tttgaatatc aatcccgata tagttgatgt cagggttttg cttggccata
     6421 cctgaaacaa aggcaccctt tccacttcca acttccacat gaatgggatt atcattgcca
     6481 aacaagtccc gccattttgc cttggcttcc aagggattga ggaccacata ctggggattt
     6541 gcctctagta attctgttgc ccctttacga tttctaactc tcatcttctc tttccatact
     6601 tgtctcggaa atgacgcaaa ccatgaatct cccgattgac attttctaaa tcttggttca
     6661 tataatactt ggaaatctga ctcaaataag acaattgacc ataccaatac aatttactta
     6721 ataccgtttg attgtacttg taaccgtagt aggtcaacca ttccttccac tgatgttctg
     6781 aaatataatg gcagagcata tgggccacat caaacatgcg atcggtcaag cgaaccgaat
     6841 cccaatctac taaataaatc aagccactat ctgtctcaat ccaattacta tgtcgtacat
     6901 ctccatggac aatggtcgca tggtcctctc taaatcctgg aatagtctga cgtaaatcag
     6961 ccatcacttc actgataaaa tgatttttac gcaaagcatc tggagccgtt tcctgccaag
     7021 actgtagtaa atctacaggt gtttccatgg catagcccaa acgactcaat tgtgtcatca
     7081 acggacgtga gcgatgaaga cgggttaaaa tattgacgat ttgcttacga ttcatatcat
     7141 agggggtcaa tatcttgcct gtcaaccatt cttgagcaca catatcacgc ccatctgcca
     7201 aacgacgact ccataataat tgtggagcaa tttgttctct agctagacca ggtaggattg
     7261 gagaggtgtt catttttaca aagatgcgct tcccgtcagg atagctaccc atataagcct
     7321 tgccactttt cccaggtatg ggagtcagtg ttagctcatt atcacccaaa tccatttctt
     7381 tcctccgtat aaagtttcct tactattcta ctagtttttg gcctattcgt caaccactct
     7441 aacccgattc tcaaagaaaa gcctctggat tggcttgggt atcattatag cttgaaaaag
     7501 gtaagcacca tcttctgctt accttttcat aatttttata aataatatag gagtggccaa
     7561 ctgttgtaaa acatgtggac tagagtagaa acccataggt tttgactctt tttataagca
     7621 aagcccagca acaaaccccc aagtagataa aaaataaacg aagggacatt agaaggtcca
     7681 tgcatgaaag aaaagagaga ggaagttagc acaagcccta accaagaatt gtcaaaaacc
     7741 gcaccttgaa gaagtcctct gaaaatgagt tcctcctgga tggggccaaa aactgaggta
     7801 gtcaaaatca aagacaggga aattcctctg ctgacttccg ccaagtgttg gacccctgag
     7861 ccagaagaaa cagcgaagaa atgaatataa accagcgtct caagtacact tagaagaaag
     7921 attgcaatga aaagcaaaag ttgtttcttg cttaacatcc caaaagaaat catttcaaac
     7981 tttctagcat aagcaacact cagcgaggtt acaagaagac ttacaagaat caatgcagat
     8041 tcattttgaa aaaaaccccc atggttaatc gtataaggaa ctgtaatgac aattattagt
     8101 actaaaaatc caaaacacaa ggcatgaaat ttat
//
LOCUS       DAVKGU010000084         7805 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKGU010000084 DAVKGU010000000
VERSION     DAVKGU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.085"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            34..6465
                     /locus_tag="VW077_001735"
     CDS             34..6465
                     /locus_tag="VW077_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9455718.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKGLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNEGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKYKEEDDFVTGGKRTVELDYKYDDKGNITAY
                     EDESALEYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIEI
                     KATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDDRIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
     gene            7092..7568
                     /locus_tag="VW077_001736"
     CDS             7092..7568
                     /locus_tag="VW077_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9455719.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            7606..>7805
                     /locus_tag="VW077_001737"
     CDS             7606..>7805
                     /locus_tag="VW077_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU9455720.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPG"
BASE COUNT         3038 a         1083 c         1514 g         2170 t
ORIGIN      
        1 attggaagtt aaataaatat gtaaggaatt aaaatgaaaa aaagtacagt attgtcatta
       61 accacagctg cagttatttt agcagcctat gcccctaatg aggtagtctt agcagacaca
      121 tctagctctg aagatgcttt aaacatttct gataaagaaa aagtagtatt agataaggaa
      181 acagaaaata aagagaaaca tgaagatatt cataatgcta tagaaacttc aaaggatact
      241 gaagaaaaga aaacaacagt tattgaggaa aaagaagttg ttagtaaaaa tcctgtgata
      301 gacactaaaa ctagcaatga agaagcaaga atcaaagaag attccaatca atcccaagga
      361 gataatgcac actcgtctgc aaataaaggc acagaaaatc cgaaaaaaga agatagactt
      421 gtctatattg ctgaatttaa agataaagaa tctggagaaa aagcaatcaa gggactatca
      481 aatcttaaga atacaaaagt tttatatact tatgatagaa tttttaacgg tagtgccata
      541 gaaacaactc cagataactt ggacaaaatt aaacaaatag aaggtatttc atcggttgaa
      601 agggcacaaa aagtccaacc catgatgaat catgccagaa aggaaattgg agttgaggaa
      661 gctattgatt acctaaagtc tatcaatgct ccgtttggga aaaattttga tggtagaggt
      721 atggtcattt caaatatcga tactggaaca gattatagac ataaggctat gagaatcgat
      781 gatgatgcca aagcctcaat gagatttaaa aaagaagact taaaaggtac tgataaaaat
      841 tattggttga gtgataaaat ccctcatgcg ttcaattatt ataatggtgg caaaatcact
      901 gtagaaaaat atgatgatgg aagggattat tttgacccac atgggatgca tattgcaggg
      961 attcttgctg gaaatgatac tgaacaagat atcaaaaact ttaacggcat agatggaatt
     1021 gcacctaatg cacaaatttt ctcttacaaa atgtattctg acgcaggatc tgggtttgcg
     1081 ggtgatgaaa caatgtttca tgctattgaa gattctatca aacacaacgt tgatgttgtt
     1141 tcggtatcat ctggttttac aggaacaggt cttgtaggtg agaaatattg gcaagctatt
     1201 cgggcattaa gaaaagcagg cattccaatg gttgtcgcta cgggtaacta tgcgacttct
     1261 gcttcaagtt cttcatggga tttagtagca aataatcatc tgaaaatgac cgacactgga
     1321 aatgtaacac gaactgcagc acatgaagat gcgatagcgg tcgcttctgc taaaaatcaa
     1381 acagttgagt ttgataaagt taacataggt ggagaaagtt ttaaatacag aaatataggg
     1441 gcctttttcg ataagaataa aatcacaaca aatgaagatg gaacaaaagc tcctagtaaa
     1501 ttaaaatttg tatatatagg caaggggcaa gaccaagatt tgataggttt ggatcttagg
     1561 ggcaaaattg cagtaatgga tagaatttat acaaaggatt taaaaaatgc ttttaaaaaa
     1621 gctatggata agggtgcacg cgccattatg gttgtaaata ctgtaaatta ctacaataga
     1681 gataattgga cagagcttcc agctatggga tatgaagcgg atgaaggtac taaaagtcaa
     1741 gtgttttcaa tttcaggaga tgatggtgta aagctatgga acatgattaa tcctaataaa
     1801 aaaactgaag tcaaaagaaa taataaagaa gattttaaag ataaattgga gcaatactat
     1861 ccaattgata tggaaagttt taattccaac aaaccgaatg taggtgacga aaaagagatt
     1921 gactttaagt ttgcacctga cacagacaaa gaactctata aagaagatat catcgttcca
     1981 gcaggatcta catcttgggg gccaagaata gatttacttt taaaacccga tgtttcagca
     2041 cctggtaaaa atattaaatc cacgcttaat gttattaatg gcaaatcaac ttatggctat
     2101 atgtcaggaa ctagtatggc gactccaatc gtggcagctt ctactgtttt gattagaccg
     2161 aaattaaagg aaatgcttga aagacctgta ttgaaaaatc ttaagggaga tgacaaaata
     2221 gatcttacaa gtcttacaaa aattgcccta caaaatactg cgcgacctat gatggatgca
     2281 acttcttgga aagaaaaaag tcaatacttt gcatcaccta gacaacaggg agcaggccta
     2341 attaatgtgg ccaatgcttt gagaaatgaa gttgtagcaa ctttcaaaaa cactgattct
     2401 aaaggtttgg taaactcata tggttccatt tctcttaaag aaataaaagg tgataaaaaa
     2461 tactttacaa tcaagcttca caatacatca aacagacctt tgacttttaa agtttcagca
     2521 tcagcgataa ctacagattc tttaactgac agattaaaac ttgatgaaac atataaagat
     2581 gaaaaatctc cagatggtaa gcaaattgtt ccagaaattc acccagaaaa agtcaaagga
     2641 gcaaatatca catttgagca tgatactttc actataggcg caaattctag ctttgatttg
     2701 aatgcggtta taaatgttgg agaggccaaa aacaaaaata aatttgtaga atcatttatt
     2761 cattttgagt cagtggaaga aatggaagct ctaaactcca acgggaagaa aataaacttc
     2821 caaccttctt tgtcgatgcc tctaatggga tttgctggga attggaacca cgaaccaatc
     2881 cttgataaat gggcttggga agaagggtca agatcaaaaa cactgggagg ttatgatgat
     2941 gatggtaaac cgaaaattcc aggaacctta aataagggaa ttggtggaga acatggtata
     3001 gataaattta atccagcagg agttatacaa aatagaaaag ataaaaatac aacatccctg
     3061 gatcaaaatc cagaattatt tgctttcaat aacgaaggga tcaacgctcc atcatcaagt
     3121 ggttctaaga ttgctaacat ttatccttta gattcaaatg gaaatcctca agatgctcaa
     3181 cttgaaagag gattaacacc ttctccactt gtattaagaa gtgcagaaga aggattgatt
     3241 tcaatagtaa atacaaataa agagggagaa aatcaaagag acttaaaagt catttcgaga
     3301 gaacacttta ttagaggaat tttaaattct aaaagcaatg atgcaaaggg aatcaaatca
     3361 tctaaactaa aagtttgggg tgacttgaag tgggatggac tcatctataa tcctagaggt
     3421 agagaagaaa atgcaccaga aagtaaggat aatcaagatc ctgctactaa gataagaggt
     3481 caatttgaac cgattgcaga aggtcaatat ttctataaat ttaaatatag attaactaaa
     3541 gattacccat ggcaggtttc ctatattcct gtaaaaattg ataacaccgc ccctaagatt
     3601 gtttcggttg atttttcaaa tcctgaaaaa attaagttga ttacaaagga tacttatcat
     3661 aaggtaaaag atcagtataa gaatgaaacg ctatttgcga gagatcaaaa agaacatcct
     3721 gaaaaatttg acgagattgc gaacgaagtt tggtatgctg gcgccgctct tgttaatgaa
     3781 gatggagagg ttgaaaaaaa tcttgaagta acttacgcag gtgagggtca aggaagaaat
     3841 agaaaacttg acaaagacgg aaataccatt tatgaaatta aaggtgcggg agatttaagg
     3901 ggaaaaatca ttgaagtcat tgcattagat ggttctagca atttcacaaa gattcataga
     3961 attaaatttg ctaatcaggc tgatgaaaag gggatgattt cctattatct agtagatcct
     4021 gatcaagatt catctaaata tcaaaagctt ggcgagattg cagaatctaa atttaaaaat
     4081 ttaggaaatg gaaaagaggg tagtctaaaa aaagatacaa ctggggtaga acatcatcat
     4141 caagaaaatg aagagtctat taaagaaaaa tctagtttta ctattgatag aaatatttca
     4201 acaattagag actttgaaaa taaagaccta aagaaactca ttaaaaagaa atataaggaa
     4261 gaagacgact ttgtaactgg tggaaaaaga acagtagaac ttgattataa atatgacgat
     4321 aaaggaaata taactgccta tgaagatgaa agtgctctag aatatgaaac tgaaaaattt
     4381 gacgaaataa aatcaaaaat ttatggtgtt ctaagcccat ctaaagatgg acactttgaa
     4441 attcttggaa agataagcaa tgtttctaaa aatgccaagg tatattatgg aaataactat
     4501 aaatttatag aaatcaaagc gactaagtat gactcacatt caaaaacgat gacatttgat
     4561 ttatacgcta atattaatga tattgtggat ggattagctt ttgctggaga tatgcggttt
     4621 tttgttaaag atgatgatcg aataaaagct gaaactaaaa ttagaatgcc tgaaaaaaat
     4681 aaggaaacta aagcagaata tccctatgta tcaagttatg ggaatgtaat cgaattagga
     4741 gaaggagatc tttcaaagaa caaaccagac aatttaacta aaatggaatc tggtaaaatc
     4801 tattctgatt cagaaaaaca acaatatctg ttaaaggata acatcattct aagaaaaggc
     4861 tatgcactaa aagtgactac ctataatcct ggaaaaacgg atatgttaga agggaatgga
     4921 gtctatagca aggaagatat agcaaaaata caaaaggcca atcctaatct aagagtcctt
     4981 tcagaaacaa caatttatgc tgatagtaga aatgttgaag atggaagaag tacccaagct
     5041 gtattaatgt cggctttgga cggctttaac attataaggt atcaagtgtt tacatttaaa
     5101 atgaacgata aaggggaagc tatcgataaa gacggaaatc ttgtgacaga ttcttctaaa
     5161 cttgtattat ttggtaagga tgataaagaa tacactggag aggataagtc caatgtagaa
     5221 gctataaaag aagatggctc catgttattt attgatacca aaccagtaaa cctttcaatg
     5281 gataagaact actttaatcc atctaaatct aataaaattt atgtacgaaa tccagaattt
     5341 tatttaagag gtaagatttc tgataagggt ggttttaact gggaattgag agttaatgaa
     5401 tcggttgtag ataattattt aatctacgga gatttacaca ttgataacac tagagatttt
     5461 aatattaagt tgaatgttaa agacggtgac atcatggact ggggaatgaa agactataaa
     5521 gcaaacggat ttccagataa ggtaacagat atggatggaa atgtttatct tcaaactggc
     5581 tatagcgatt tgaatgctaa agcagttgga gtccactatc agtttttata tgataatgtt
     5641 aaacccgaag taaacattga tcctaaggga aatactagta tcgaatatgc tgatggaaaa
     5701 tctgtagtct ttaacatcaa tgataaaaga aataatggat tcgatggtga gattcaagaa
     5761 caacatattt atgtaaatgg aaaagaatat acatcatttg atgatattaa acaaataaca
     5821 gacaagacac taaacattaa gattgttgta aaagattttg caagaaatac aaccgtaaaa
     5881 gaattcattt taaataaaga tacgggagag gtaagtgaat taaaacctca tagggtaact
     5941 gtgaccattc aaaatggaaa agaaatgagt tcaacgatag tgtcggaaga agattttatt
     6001 ttacctgttt ataagggtga attagaaaaa ggataccaat ttgatggttg ggaaatttct
     6061 ggtttcgaag gtaaaaaaga cgctggctat gttattaatc tatcaaaaga tacctttata
     6121 aaacctgtat tcaagaaaat agaggagaaa aaggaggaag aaaataaacc tacttttgat
     6181 gtatcgaaaa agaaagataa cccacaagta aaccatagtc aattaaatga aagtcacaga
     6241 aaagaggatt tacaaagaga agatcattca caaaaatctg attcaactaa ggatgttaca
     6301 gctacagttc ttgataaaaa caatatcagt agtaaatcaa ctactaacaa tcctaataag
     6361 ttgccaaaaa ctggaacagc aagcggagcc cagacactat tagctgccgg aataatgttt
     6421 atagtaggaa tttttcttgg attgaagaaa aaaaatcaag attaagataa aagctataga
     6481 aaaaaatggt ttatgtactg agattagata gtgaggtgat gacatagttt tgtgaaaaca
     6541 gccatttata actcaattat ttagtttact tcactattga tactatttgg agttattaat
     6601 ggacttagtt tagataacta atgaattgat tgaaagggtt agtattgaca atattggtca
     6661 tattgattag aaaatagagt ctatcaaaat ttaaaggcta atagaggtga tgagacaatt
     6721 tcaaggaatt aagtagcgtt tggttgctta attccttttt atttggaagg aagtttttcg
     6781 taaccgccca ataacgaagt ctattgggcg gttacgatat gatcactgac ttcgtcagtt
     6841 ctatctacaa cctaaaaaca gtgttttgaa caacctgcgg ctagcttcct agtttgctct
     6901 ttgattttca ttgaatatta gaacagcaaa aatgcttgga gtatttgttt gtgtgtttgt
     6961 ttttatataa caaactataa acaaaacaaa aatataaaaa aagagacaaa aaagaacaaa
     7021 aagtaattga caacggtttc atatagtgtt atacttatgg cataaagtta atggaaagaa
     7081 gggaaaacct tatggtatta gattatttct ttgacaaaaa ccttgtgttt tgcttagaag
     7141 cggataatca agaacaactc tttgatcagg ttgcaacttt attggaagaa cgagaaattg
     7201 tcactccaac ttatcgtgaa gccttgatca cgcgtgaaaa gtcatttcca actggtttag
     7261 atatggaatt tctaggaaag gacttgccaa atgtagcgat tcctcataca gatattgttc
     7321 ataatctagc tgagaaagtg gtggttgttc gattagaaaa accagtaact tttcacaata
     7381 tgatagctcc tgataaggaa gtagaagtat ccttgctctt ctttatcatc aataactcaa
     7441 gttcaagtca aacaaatatt ctggctcagt tgatggactt tttcacagga aatggacatc
     7501 ttgaagacct atcaaaaatt tccgaaccag aaaaacttta tgcttacatt gctgaagcaa
     7561 ccgcttaatc ttgtctatta aaaaataaaa tcggaggaaa tctaaatgat taaaattctt
     7621 gctgcctgcg gtgcaggtgt taactcaagt caccaaatta aaagtgctct agaagaagaa
     7681 ctttcaaacc gtggttatga tgttcactgt gatgcagtca tggtgaaaga tgtaaacgaa
     7741 gaccttatga aaggttacga tatctttaca ccaatcgctg caacagacct tggttttgaa
     7801 ccagg
//
LOCUS       DAVKGU010000085         7763 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKGU010000085 DAVKGU010000000
VERSION     DAVKGU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.086"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(52..3894)
                     /gene="spuA"
                     /locus_tag="VW077_001738"
     CDS             complement(52..3894)
                     /gene="spuA"
                     /locus_tag="VW077_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9455721.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATSPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVDAKKEDILKHSNITNHL
                     GNKVTITDVAIDETGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLREEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRKESVENSSKENISATPDRQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            4311..4724
                     /gene="rpsL"
                     /locus_tag="VW077_001739"
     CDS             4311..4724
                     /gene="rpsL"
                     /locus_tag="VW077_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9455722.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            4744..5214
                     /gene="rpsG"
                     /locus_tag="VW077_001740"
     CDS             4744..5214
                     /gene="rpsG"
                     /locus_tag="VW077_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9455723.1"
                     /translation="MSRKNRAPKRDVLPDSLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            5639..7720
                     /gene="fusA"
                     /locus_tag="VW077_001741"
     CDS             5639..7720
                     /gene="fusA"
                     /locus_tag="VW077_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9455724.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
BASE COUNT         2124 a         1558 c         1735 g         2346 t
ORIGIN      
        1 ttaatccccc gagctataat gcttggggga tttttctata ggagggctag cttagttctc
       61 ttttttattt tttagtaaga aaccgagacc aaggagcgca aggaggctga ttcctgcaaa
      121 taggagtttg ttttcgtttt tgattcctgt atttggaagt tcagcttgtc tatctggggt
      181 tgcagatata ttttccttgc tagagttttc taccgattct tttcgaaccg cttcttttac
      241 agatgatgct tgtgcttctt gtgctggttg ttcagcttgt gaatcagctg tagcttggct
      301 aggtttattt tccgcatcag ctactttggc atctggttta gtagaatcag gtctagcttc
      361 tggagctggg tcttgatgtg cagggtgaga agcttcattt tgatgttcag gcttggaagg
      421 ggttgagtct ggtttctctt ctgcagttga ctcatggcta gttccatttt gagagactcg
      481 aagaacagta gctgtaaggg cattcaattt caagcctttt tcagtccatt caagtccttt
      541 cgggttggca attccgactg atcctgcttg gttttcatct gccaaaactt ccgcatttct
      601 tagatgtgca aaggcagttc ccaaattaaa ttcgcgagct ttttcatccg cattgacaaa
      661 gactgcgtag atatcgccgt ttggagcagt gatttggtag ccaatcacta catcctcttt
      721 ttccacacca ttttggcctg ggacagtgat gaggtggaca cggtctttga tatcttggag
      781 actcttaagt cggaaggcat ctgtagattg acgaagggca atcaaacctt tcatatagtc
      841 acggctcttg acattttcag gataagcttt accatctgta gccttagtcc agtcaaactt
      901 gttgactgcg tcgctagaat cgtaagaatc atggatgaag taaggatagt caaatgggtt
      961 gccgtcctta tcacgcaaca agtgagattt gtttggaacc ttatcctctg ctactggagt
     1021 cttgtaggct gggtcacgga attgtttagt acgtccatat tcctgaccgg agtggataaa
     1081 tggagttcct tgagctgtca agaccatgag atttccaagt cgtaaacgac ggtggatttc
     1141 agcatagttc tcagccttgc ttgggtcttt tttgatagac tgggcaatga tgtcaaagag
     1201 ggtcaagtta tcatgggctg cgatgtattg gatgacatct ccagggctgt cagcttcaaa
     1261 gttagttggt tgagcaatga gatttttaaa gatggtgttg acatcacgct tgccacctgt
     1321 gataaaggca ggttgacctt cgtttggata accagatttg aggttgttac ggatgtcatc
     1381 tgaaaagaca gcgacagtat cggtatgttt catccaatct tggtcagcag ctttagtagg
     1441 catgttttca tcaccggcat aggttctcca accttcgcct aacatgatta gatttggatt
     1501 gagggcgcgt gcagccttgt aagcttcttc gatagaagcg gcgtcatggt ctcccatcat
     1561 atcgaagcgg aagccatcca ctttgtaggt atcaactagg tatttgatag agtcaactag
     1621 gagccgtttg gtcatatggt gggttgtccc caagcgtcca ccaccaaagc tagttcgagg
     1681 tgtgccatcg gcatccataa agtggtagta gtttggctct aggtcttcaa agatatcgac
     1741 tttggctgtg tggttataaa cgacatctag gatagctccc ataccacgtt tgtggatttc
     1801 gttgatgagg tttttaaatt ctgcgattcg tttttctgga ttcttaggat cgcttgagta
     1861 cataccagtc aaggagaagt agttttgagg gtcatatccc cagttgtagt tgctgttgct
     1921 tgaagcatag tcagacaagc gttcatggtt tttcaattca ttgacaaagt agtaagacaa
     1981 gactggaagg agctggatat gggttacacc caagtctttg agatagtcta gtttttcaat
     2041 gaaggcttca aaagtcccaa atggtttggt caagtctttt gcaatggcag gatctgaagt
     2101 gaagtcacgc acatgagctt cgtagataac ggcgtcttca cgagtcttga aattgtgaat
     2161 cttaccataa gtcaagtctt gaggtccgag tttagctgga tctacaaagg cggctttagc
     2221 cactttatgg gcatcgtcaa tcttggcatc gtcgctattc caagcagcaa gagatttagc
     2281 gtaaggatcg agtgcaagaa cagttttacc ttgacgctcg atttggtatt gataatagta
     2341 gccagtgaaa tctgtgattc cgagtttgtt tgtgctgtct agagtttgtt tccaagttcc
     2401 tctttcccct ttttcgagag cgacagttcc aactactttg tcagggtcat tcttgtcgta
     2461 gacaacaaca gaaaccttat cagcacttgg tgaccaaagg gtcaaatcaa cttgttttcc
     2521 ttcttctctt aggtcagctc ccagtttgcc atcatagctg tatgtctcat ctttcaggcg
     2581 ccagcttgtt ttggtagtga attgatcgga attgtagcta acagtataag gatgttttgt
     2641 gtcagagaaa tctccgctgt aggtcacttt cttaccagtt tcatcgattg caacatcggt
     2701 aatagttacc ttgtttccta ggtgattagt gatgttggag tgtttgagga tatcttcttt
     2761 tttagcatcg acaagtgttg aaaagctact ttcaatgcta gaagtgccta cgtgttgggc
     2821 tcctgtcata cggatatcat ggacatagta tggatttgtg taaatcgatt catcatcgtc
     2881 ttttaggaaa atttggctat gatttttcaa atctgtgaac ttataatttt ctttacggat
     2941 tttcacgtcg tctccttgtt tgctctcatc tagtaataaa aatccaaatt ctcttgcggc
     3001 ttcattaaga ggaatgtcga tatagcggcc atatttgcct gtagccgtaa agtctgttcc
     3061 gtcaggccat tgagcgctac ttggattttt tacatctccc cagtaccaga gagatttctt
     3121 gtcatagttg ccatctgtgc ggtagtagtt gacgcgaaca gttcctgcag gctgtggctc
     3181 gtaagagaaa accttgtaat cttggtctaa ccaagcttcg ttcatttttg gaactagttt
     3241 ttctacagat ttatcgccgg ttagattttt tccagctgta ttgttgatga ggaagctaat
     3301 tttcttggct tgttctccct ttaatttgac atctaggtaa tagccgtagt catctttctt
     3361 ggcatccttg aaggacaaag ctccgtttgg ccagttttca gatggttttt caacatcgtc
     3421 ccaagtccat agtccttgag catccttgtt ttcttcagga agttttttga catggatacg
     3481 gaagtagtta tcttcgatgg gctcttctgc cttagtttca gttgttactg gactagtaga
     3541 agtagttggc tcacttgaac gatcttgtcc agtttgtggt gctggatcgg ctgggcttgt
     3601 agcaggtttt tcttctgttt tctctaggga agcgtttgca tttctaggtg aagaaatatc
     3661 actttcgttc ttaccagagc cagctgctgt atcagtaaga ggttgagcaa gggtggttgt
     3721 atttctgttg ttgggttcgt tagtggttgg tgtactttcg tcggatgaga tagttggtgt
     3781 agccatggca agcaagacaa ggcttgcgcc gataaggaca gaaccagttc catttttgag
     3841 ggaacggatg ctgtagacca tttttttctc agtgtgagat ggtgtttttc tcataatgat
     3901 tctccaatca ataaatttct atatcagtat agcatgtagt aaaacaatat gcaagcgttt
     3961 tctttgattt gtgaaaaaaa ttcctacttt tgagaagttt tctgtactat tttatatatc
     4021 agtataaatg acggagataa aaatgtcaca taagccaagc cgtattatta ggaagatgat
     4081 gtgttcccag ctatttttat tcagaaattg atctctaaca ttaccaataa aaatgaaaaa
     4141 tatcttgaca agcaagggaa aattttgtta caataataga cggtactttt tacttttggt
     4201 ctctcaagag tgtacaggga cgtgctgaca aatgttgcaa aagtacacac agatgatatc
     4261 tgtcaccaag tgtatcatca ccaaaaataa aaaaacacag gagaatgtag atgcctacaa
     4321 ttaaccaatt ggttcgcaaa ccgcgtaaat caaaagtaga aaaatctaaa tcaccagctt
     4381 tgaacgttgg ttacaatagt cataaaaaag ttcaaacaaa cgtttcttca ccacaaaaac
     4441 gtggtgttgc aactcgtgtt ggaacaatga cacctaaaaa acctaactca gcccttcgta
     4501 aattcgctcg tgtacgtttg agcaacctta tcgaagttac tgcctacatc ccaggtatcg
     4561 gacacaactt gcaagagcac agcgtggtgc ttcttcgcgg tggacgtgta aaagaccttc
     4621 caggggtacg ttaccatatc gtccgtggtg cacttgatac tgcaggtgtt aacgatcgta
     4681 aacaaggccg ttctaaatac ggtactaaac gtccaaaagc ataaggaaag gggataaaga
     4741 gaaatgagtc gtaaaaatag agctccaaaa cgtgacgtat tgccagattc gctatacaat
     4801 tcacaactag ttactcgtct tatcaaccgc gttatgcttg atggtaaacg tggtactgct
     4861 gcttcaatcg tttacggtgc ttttgagcaa atcaaagaag ctactggcaa cgatgcactt
     4921 gaagtatttg aaacagctat ggaaaacatc atgcctgtac ttgaagtacg tgcacgtcgt
     4981 gttggtggtt ctaactacca agtcccagtt gaagttcgtc cagaacgtcg tacaacactt
     5041 ggacttcgtt ggttggtaac aatcgctcgt cttcgtggtg aacacacaat gcaagaccgt
     5101 cttgcaaaag aaatcttgga tgctgctaac aacactggtg cagcagttaa gaaacgtgaa
     5161 gatactcacc gtatggctga agctaaccgt gcattcgcac acttccgttg gtaagatagg
     5221 atgcgaaagc gttaagaaag tcccagagaa aatagggaat cgaagcaggt tgcggttgca
     5281 accaatgaga ttcatctttt tctccagact tttagcttga gctcaactaa atcatgatgc
     5341 taggcacggt aaggatgcaa ggtgaaaata ggaaactgac gcagtattca acgaatacaa
     5401 ggagttttat ctttttcacg cagcatcccg ttccagctca catcggctaa ctaactttag
     5461 cctgggttca aattagctaa atcgattagt attagctata actcagctta ccatctcgta
     5521 agttgaaacc aacaatagca tgaaaacatt gagaacgggt aggtcctgcc tatccgtttt
     5581 tattaaaatc gtgttataat agaatagaaa tcaaaaataa ataggagaaa caaacctcat
     5641 ggcacgcgaa ttttcacttg aaaaaactcg taatatcggt atcatggctc acgtcgatgc
     5701 cggtaaaaca acaactactg agcgtattct ttactacact ggtaaaatcc acaaaatcgg
     5761 tgaaactcac gaaggtgcgt cacaaatgga ctggatggag caagagcaag aacgtggtat
     5821 cacgatcaca tctgctgcga caacagctca atggaacaac caccgcgtaa acatcatcga
     5881 cacaccagga cacgtggact tcacaatcga agtacaacgt tctcttcgtg tattggatgg
     5941 tgcggttacc gttcttgact cacaatcagg tgttgaacct caaactgaaa cagtttggcg
     6001 tcaagcaact gagtacggag ttccacgtat cgtatttgcc aacaaaatgg acaaaatcgg
     6061 tgctgacttc ctttactctg taagcacact tcacgatcgt cttcaagcaa atgcacaccc
     6121 aatccaattg ccaatcggtt ctgaagatga cttccgtggt atcattgact tgatcaagat
     6181 gaaagctgaa atctatacta acgaccttgg tacggatatc cttgaagaag acatcccagc
     6241 tgaatacctt gaccaagctc aagaataccg tgaaaaattg attgaagcag ttgctgaaac
     6301 tgacgaagaa ttgatgatga aatacctcga aggtgaagaa atcactaacg aagaattgaa
     6361 agctggtatc cgtaaagcga ctatcaacgt tgaattcttc ccagtattgt gtggttcagc
     6421 cttcaaaaac aaaggtgttc aattgatgct tgatgcggtt atcgactacc ttccaagtcc
     6481 acttgacatc ccagcaatca aaggtattaa cccagataca gacgctgaag aaactcgtcc
     6541 agcatctgac gaagagccat ttgcagctct tgccttcaag atcatgactg acccattcgt
     6601 aggtcgtttg acattcttcc gtgtttactc aggtgttctt caatcaggtt catacgtatt
     6661 gaatacttct aaaggtaaac gtgaacgtat cggacgtatc cttcaaatgc acgctaacag
     6721 ccgtcaagaa atcgacactg tttactcagg tgatatcgct gctgccgttg gtttgaaaga
     6781 tactacaact ggtgactcat tgacagatga aaaatctaaa atcatccttg agtcaatcaa
     6841 cgttccagaa ccagttatcc aattgatggt tgagccaaaa tctaaagctg accaagacaa
     6901 gatgggtatc gcccttcaaa aattggctga agaagatcca acattccgcg ttgaaacaaa
     6961 cgttgaaact ggtgaaacag ttatctcagg tatgggtgaa cttcaccttg acgtccttgt
     7021 tgatcgtatg cgtcgtgagt tcaaagttga agcgaacgta ggtgctcctc aagtatctta
     7081 ccgtgaaaca ttccgcgctt ctactcaagc acgtggattc ttcaaacgtc agtctggtgg
     7141 taaaggtcaa ttcggtgatg tgtggattga atttactcca aacgaagaag gtaaaggatt
     7201 cgaattcgaa aacgcaatcg tcggtggtgt ggttcctcgt gaatttatcc cagcggttga
     7261 aaaaggtttg gtagaatcta tggctaacgg tgttcttgca ggttacccaa tggttgacgt
     7321 taaagctaag ctttatgatg gttcatatca cgatgtcgac tcatctgaaa ctgccttcaa
     7381 gattgcggct tcactttccc ttaaagaagc tgctaaatca gcacaaccag ctatccttga
     7441 accaatgatg cttgtaacaa tcactgttcc agaagaaaac cttggtgatg ttatgggtca
     7501 cgtaactgct cgtcgtggac gtgtagatgg tatggaagca cacggtaaca gccaaatcgt
     7561 tcgtgcttac gttccacttg ctgaaatgtt cggttacgca acagttcttc gttctgcatc
     7621 tcaaggacgt ggtacattca tgatggtatt tgaccactac gaagatgtac ctaagtcagt
     7681 acaagaagaa attattaaga aaaataaagg tgaagactaa tccgtcctca ctctagaagg
     7741 aagtcactta gtggcttcct ttt
//
LOCUS       DAVKGU010000086         7560 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKGU010000086 DAVKGU010000000
VERSION     DAVKGU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.087"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            107..295
                     /locus_tag="VW077_001742"
     CDS             107..295
                     /locus_tag="VW077_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9455725.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            357..1115
                     /locus_tag="VW077_001743"
     CDS             357..1115
                     /locus_tag="VW077_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9455726.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNETHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            1173..2027
                     /locus_tag="VW077_001744"
     CDS             1173..2027
                     /locus_tag="VW077_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9455727.1"
                     /translation="MLGVIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVT
                     EAIMNGGQKDDFIDAMKKYGSMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            2139..3023
                     /locus_tag="VW077_001745"
     CDS             2139..3023
                     /locus_tag="VW077_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9455728.1"
                     /translation="MKKVMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3186..3263
                     /locus_tag="VW077_001746"
     CDS             3186..3263
                     /locus_tag="VW077_001746"
                     /inference="COORDINATES: protein motif:HMM:NF014902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU9455729.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHI"
     gene            3327..4925
                     /locus_tag="VW077_001747"
                     /pseudo
     CDS             3327..4925
                     /locus_tag="VW077_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            5200..5655
                     /locus_tag="VW077_001748"
     CDS             5200..5655
                     /locus_tag="VW077_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455730.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            6100..6576
                     /locus_tag="VW077_001749"
     CDS             6100..6576
                     /locus_tag="VW077_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9455731.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            6576..7334
                     /locus_tag="VW077_001750"
     CDS             6576..7334
                     /locus_tag="VW077_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9455732.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAATVLHDLLFGELNQVEKEMLKSGEERLKDLTN"
BASE COUNT         2594 a         1089 c         1552 g         2325 t
ORIGIN      
        1 taaagttact gtgttaaaaa tcctctccac ttttaaacta atgtattata aaagccagtt
       61 gacacaagtg tgacacttgt gatagaatta acttgaggtg atgaaaatgg caaccgataa
      121 aaatagaata atgattagct tggatgataa aaatctagag aaacttgaaa atttagtaga
      181 agatgctaga gatagaaggg gaatgcgact aacaaagtcc caggttattg aattactcct
      241 aaataccgtt gattatttcg atgatattat gggggctatt tactcaaaaa aataagagca
      301 aaattgctat tattttttac aaattggtgt aacactagtg atataggagt gatgaaatgg
      361 ttgtttatat tagacaaagt aaattaccaa gtgaggtttc aattaataaa tacaatgctc
      421 aagtaggtgc ctacttacaa ggagaagaag ttattttata tcaatctttt tctgaaataa
      481 aagagttaac aagtgaagat atagttgtag attatataat ggagactaga gcattactaa
      541 aaatgatggg cttaaacgtt ccggtttatg actatcctat tgagcttaaa gagttttatg
      601 gtcgaaagat ttatgctggt attttaggag agattgtgaa tatacctgat aattggggaa
      661 aatttattaa gcctaaagct ggttcaaaag tctttactgg aagagttgtt aatgaaaccc
      721 atgatttaat aggtattggt ctacctttcg actatcctat atggattagt gaggttgtag
      781 aattcatagc tgaatggcgt tgttttgtgt tagatggtcg cgtattagat gttcgaccct
      841 atacaggtga ttatcatgca caatttgatg caagtgtaat tgatgaagcg atatcatgtt
      901 ggaaagatgc gccaatagct tatggactag atatcggtgt tactcgcgat ggcagaacac
      961 ttgttgttga agtaaatgat ggttatgcat tgggaaatta tggcctatct cctttaaaat
     1021 caatcaattt tcatagagct aggtggaaag aaatggtaaa accctatttt gaaaaaaatg
     1081 aaatttttaa aatccaacag gatgttattt tctaaaataa atatggtaga taaccaatat
     1141 aaaaagcatc aaaatgaaca aggaggaatc tgatgcttgg agtaattatt ggagatattg
     1201 tcggttctgt ttacgaatgg aacaatatca aaacaaagga ctttccttta tttcggaagg
     1261 actgcttttt tacagatgac acggttatga cctgtgctgt tacagaagca attatgaatg
     1321 gtggacaaaa agacgatttc attgacgcga tgaagaaata tggcagcatg tatccgaatg
     1381 ctgattacgg tgctcggttt aatgcatggc taaacagcga taatcgtgag ccttataata
     1441 gctttgggaa tggatcagct atgcgtattt ctccatgtgc ttggatcatg gactgtggtt
     1501 tttatgcgaa aactggtatg tggccatcat ctagaggact tacgagtctt tctgcagagg
     1561 taactcataa tcatccagaa ggtgtcaaag gcgctatggc tacagctgat gctatctttc
     1621 tatgtcgttt ttactttggt ggttattgta gagagtacga gcaatcaatt aacgacaatc
     1681 ctacagagtg taaaagacga attaaggatt atatagaaaa agaatacgat tacaatctat
     1741 ctcaaacttt agatgaaatc cgtcctaact atcgttttaa cgaaacatgt caggaaactg
     1801 tccctcaagc cattatcgcc tttcttgaaa gtagagactt cgaagatgcg ataagaaatg
     1861 ccatctcact tggtggcgac agtgatactc tcgcagcaat cacttgtaac atagcagagg
     1921 cagcttacgg tattcctgat tggatcaagg ataaggccta ttcttacttg gatgaaccct
     1981 taaaggatgt agttaggcga tgggaaaata gaataaaagc gtattaatct ccagtacgtc
     2041 agatttctct tttctgtatt ttcattgtat aataggatta catttacttg catacgacga
     2101 aatgtaaaca tttctgttta aaattaagga taaaatcaat gaaaaaagta atggtaatta
     2161 tcaaccctac ttctggtggc gagaaggctt tggattacaa agaaaagctg gagaataaag
     2221 caaaagaata ctttgaatat gttgaaacca aaattaccga aaaagcgctg gatgcaacac
     2281 attttgctga agaagcttct cgtgagcagt atgatgcagt ggttgtgttt ggtggagatg
     2341 gaactgtcaa tgaagtcatt tcaggtattg atgagagaga ctacattcct aagttaggga
     2401 ttatcccagg cggtacgggt aacctcatta caaaactttt ggaaatcaat caagacatcg
     2461 atggcgcaat tgacgaactg gattttgatt taaccaataa gattgatatc ggtaaagcaa
     2521 atgacaacta ttttggttat atctttagta tcggttctct gcctgaggcg attcacaatg
     2581 ttgaaataga ggacaaaaca aaattcggta ttttaaccta tgctgtaaat accatgaagt
     2641 ctgtcatgac agatcaggtc tttaacatta aggttgagac agaaaatgga aattatgttg
     2701 gtgaagctag ccatgttttg gtccttttga caaattactt cgctgataag aaaatctttg
     2761 aagaaaacaa ggacggctat gccaacattt tgattctgaa agatgcctct atattctcca
     2821 aattatccgt cattcctgat ttattaaaag gggatgttgt cgcaaatgat aatatcgagt
     2881 atatcaaagc gcgtaatatt aaaatctctt cagatagtga attggagtca gatgttgacg
     2941 gagataaatc agataaccta cctgtagaaa tcaaagtcct agctcagcga gtagaagtat
     3001 tttcaaaacc gaaagaggat tagtatatag agaaagcctt ttttaaggct ttttgtatac
     3061 tttaaaagat agttccttta acaacggaca ttccttgcaa atagtttaca aaaatagtat
     3121 actggattta ttgagtttga aaacgtttgc gtaaaatttg aataaatact ttaggagaca
     3181 aattgatgga attgagtgct atttaccata ggcctgagtc ggagtatgcc tatctttata
     3241 aggataagaa actccatatt tgaattcgaa ctaagaaagg ggacattgaa agcatcaact
     3301 tgcactatgg ggaccctttt atctttatgg aggagtttta tcaggataca aaagaaatgg
     3361 tcaagataac ttctggtacc ttatttgacc attggcaggt tgaagtgtca gttgactttg
     3421 cacgtatcca gtatctcttt gagctcagag atacagaagg tcaaaatatt ttgtatggcg
     3481 ataaagggtg tgtggaaaat tctctagaaa atcttcatgc aattgggaat ggatttaagt
     3541 tgccttatct tcatgagatt gatgcctgca aggttcctga ctgggtttca aatacggtat
     3601 ggtatcagat atttcctgaa agatttgcca atggcaatgc tctattaaac ccagaaggga
     3661 ctttagactg ggattcatct gtcacaccta agagcgatga tttctttggt ggtgatttac
     3721 aggggattat tgatcgtatg gattacttgc aagacttggg tattactgga ctatatcttt
     3781 gtcccatctt tgaatctaca agcaatcaca agtacaatac gacagattac tttgaaattg
     3841 accgttattt tggagacaag gagacctttc gggaactggt ggatcaagcg catcatcgtg
     3901 gcatgaaagt catgatggat gcggtattta atcatattgg ttcgcaatct cttcaatgga
     3961 aaaatgtcgt caaaaatggc gaacagtctg cttataagga ttggttccat attcaacaat
     4021 tcccagtgac aactgaaaag ctagttaata agagagactt accctatcat gtttttggtt
     4081 tcgaggacta tatgcctaag ctaaatacag ccaatccaga ggtcaagaat tatcttttaa
     4141 aggttgcgac ttattggatt gaagagttta atatcgatgc ttggcgtttg gatgtggcta
     4201 atgagattga ccatcagttc tggaaggatt ttcgtaaggc agttttagct aaaaatcctg
     4261 atctttatat cctaggagaa gtctggcata catctcagcc ttggctaaat ggagatgagt
     4321 tccatgccgt catgaattat cctttatctg atagtatcaa ggactatttc ttacgaggaa
     4381 ttaagaagac agaccagttc atcgatgaaa tcaattgaga gtctatgtat tacaagcagc
     4441 agatttcaga ggtcatgttt aatctcttgg attcacatga tacagagcga atcctgtgga
     4501 cggccaatga agatgttcaa ctggttaaat cagccttagc ctttctcttt taacaaaaag
     4561 gaacaccgtg catttattac ggaaccgagc tagccttgac tggaggacca gatccagatt
     4621 gtcgtcgttg tatgccttgg gaacgtgtat caagtgacaa tgatatgctg aactttatga
     4681 agaggctgat taagattcgg aaatacgcgt cagtaatcat ttcgcatggc aagtatagcc
     4741 ttcaagaaat caaatctgat ctagtagctc tggaatggaa atacgaagga cggatcctca
     4801 aagcaatatt caaccaatca acagaagatt atcttttaga gaaagaagca gtagcactag
     4861 caagcaattg ccaagaattg gagaatcagc ttgtcatctc tccagatgga tttgtgattt
     4921 tctaaaaact agttgatgaa gattatggta catttcatat cttatatagt ataataagga
     4981 tagttactaa acttgtaaag gagaacttaa atgaattgta gaggacatga aacaagacaa
     5041 agaattgtta gagattttga agtttagcct aaagcacata ttaagctgtt agcaaatcaa
     5101 caaaaacata gtgatgcagg agcaactatt gaagatgaat attatgtatt tatcgctgag
     5161 agtaaaattg atggcaagaa ggaagttatt cagtgttgca tgggtgcggc aagggatttt
     5221 ttagaactaa ttaatcacaa agggctacct ctttttaatc cgcttgtagg tgattctcat
     5281 gtaaataata gacaagaata tgacaataca gggagtggaa atttacaacc tgaaaagtgg
     5341 aatgaaactg caaagcagct ttataatgtt ataatgtggt tgattatttt atggaatgct
     5401 aagccggata cacctttatt taattttaaa gacgaagtaa ttaagtataa aacatatgag
     5461 ccttttgaaa gcagtataaa aagagtaaat actactataa agaatggtag taaagggaaa
     5521 actctgactg agatgattaa tggctacaga gcggataacg atattagaga tgaaatttgt
     5581 aactttaata ttctgaaaaa taaaattcgt gatatgaaag accaacaagg aaatacaatg
     5641 gaatcttact tttagttatt gttgaatttt gggtattcta taaaatatcc taattgagat
     5701 ttaaatagta gactatacaa tatagttaaa atatcagtaa aaacaacact ttattgaggt
     5761 attggatacg ctttgctaat agcctaataa tcacatgtgg agtgttgcta caacgaaaaa
     5821 ggtgataatc cttgatttca agctatttta taagcatttt gtctttgtag ataaaggcaa
     5881 ttttgacaat aaaaatccta aaaggtgaat cgttatagat gtatttgtag atatcgtttg
     5941 cgcatcgaaa aaattaatac aagaataaat atttatagct ctttaggtga cttttataga
     6001 agtaaagttt aggatagaaa aacaagaaat aacgcaccat ttttggtgcg ttatgctttt
     6061 ttatgctata atggatttat aaaaataaag gagtttgcta tgattggaaa gaacataaaa
     6121 tccttacgta aaacacatga cttaacacaa cccgaatttg caaggattgt aggtatttca
     6181 cgaaatagtc tgagtcgtta tgaaaatgga acgagttcag tctctaccga attaatagac
     6241 atcatttgtc agaagtttaa tgtatcttat gtcgatatcg taggagaaga taaaatgctc
     6301 aatcctgttg aagattatga attgactttg aaaattgaaa ttgtgaaaga aagaggtgct
     6361 aatctattat ctcgactcta tcgttatcaa gatagtcagg gaattagtat tgatgatgaa
     6421 tctaatcctt ggattttaat gagtgatgat ctatctgact tgattcagac aaatatctat
     6481 ttagtagaaa attttgatga aatagagaga tatagcggct atttggatgg aattgaacgt
     6541 atgttagaga tatctgaaaa gcggatggta gcttaatgga aatccaagat tatactgata
     6601 gtgaattcaa acatgcttta gcaaggaatc ttcgttcact gacaagagga aaaaagtcca
     6661 gtaaacaacc tatagcgatt ttgcttggag gtcaaagtgg tgccggtaag actacaattc
     6721 atcgtattaa acagaaagaa tttcaaggaa atattgttat catagatggt gatagttttc
     6781 gttctcagca tccacactat ttagaactgc agcaagaata tggcaaagac agtgtagaat
     6841 acaccaaaga ttttgcaggg aaaatggtag agtctttagt aaccgaattg agtcatttgg
     6901 gatacaatct tttgatagag ggaactttac gaactattga tgttccaaag aaaacagcac
     6961 aactattaaa aaataagggg tatgaggtac aattagcctt aattgcaacc aagcccgaat
     7021 tgtcctatct gagcaccctt atccgatacg aagaactgta tgctatcaat ccaaatcaag
     7081 cacgcgcaac tccaaaagaa catcatgatt tcattgtaaa tcatctagtt gataatacac
     7141 gacaattgga agaactggct atctttgaaa gcattcaaat ttaccaacga gatagaagtt
     7201 gtgtatatga ttcaaaagaa aatacaactt cagcagcaac cgttcttcat gatttactat
     7261 ttggagaatt gaatcaagtg gagaaagaga tgcttaaatc aggagaagaa agattgaaag
     7321 atttaactaa ttgaaatggt tgttagaaca caaattttag aatatagtct acttataact
     7381 tttgttagtt gtttgaggtg ggcaaaaagt ttttaaaatc aaaaaaaacg catagtatca
     7441 ggtgttgaaa aaacttgata ttatgcgttt tcttgttgga agatttactc cattttcttc
     7501 aggaattgag attttgccca tctcttttta ttatatttaa ggatatttga aagctgtatt
//
LOCUS       DAVKGU010000087         7471 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKGU010000087 DAVKGU010000000
VERSION     DAVKGU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.088"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(59..1762)
                     /gene="ilvD"
                     /locus_tag="VW077_001751"
     CDS             complement(59..1762)
                     /gene="ilvD"
                     /locus_tag="VW077_001751"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9455733.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(1995..2927)
                     /locus_tag="VW077_001752"
     CDS             complement(1995..2927)
                     /locus_tag="VW077_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9455734.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2924..3781)
                     /locus_tag="VW077_001753"
     CDS             complement(2924..3781)
                     /locus_tag="VW077_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9455735.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDENKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3785..5131)
                     /locus_tag="VW077_001754"
     CDS             complement(3785..5131)
                     /locus_tag="VW077_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9455736.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(5144..5428)
                     /locus_tag="VW077_001755"
     CDS             complement(5144..5428)
                     /locus_tag="VW077_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9455737.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5432..7462)
                     /locus_tag="VW077_001756"
     CDS             complement(5432..7462)
                     /locus_tag="VW077_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9455738.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
BASE COUNT         2398 a         1643 c         1159 g         2271 t
ORIGIN      
        1 aaccatacct acggcaaggc gacgttgacg tgatttgaag agattttcga gtatgagttt
       61 attttttacc tgacttgtcc atattccaga agtctgtcac ggctccgcgt gaagcagatg
      121 atacgatgtg ggcatattta ccgaggacac cacggctgta aagtggtggc aaggttgttt
      181 ctgccttgcg tttttcaagt tcttcttcgg atacggccat ggaaatttct ttggtatctt
      241 ggtcaaccgt aacgatatcg ccggtacgga gataggcaat tggtccacca tcctgagctt
      301 caggagcgat atgtccaaca accagaccat aagtaccacc agagaaacgt ccgtccgtca
      361 agagggccac cttatctccc tgacctttac caacaatcat tgaagaaagt gacagcatct
      421 caggcatacc aggaccacct ttaggtccaa caaaacgaac aacgactaca tcgccatcaa
      481 cgatttcatc tgtcagaacg gcctgaatcg catcttcttc tgagtcaaag accttagctg
      541 gcccaacgtg acgacgcact ttaacacctg ataccttggc aactgcaccg tcaggagcaa
      601 ggttcccgtt caagatgata agcggaccat ccgcacgttt tggattttca agtggcatga
      661 taactttttg gcctggagtc aagtctgcaa agtcagccaa gttttcagct acagtcttac
      721 cagtacatgt gatgcgatct ccgtgaagga aaccatttgc caacaaatac ttcataaccg
      781 cagggacacc accgacttcg tagaggtctt ggaagacata ctgaccagat ggtttcaagt
      841 cggccaagtg aggcacacgt tcttgaatcg tattgaagtc ctcaagtgac aagtcaacat
      901 ttgcggcatg ggcaatggcg agcaagtgaa gagtggcgtt tgtagaacca ccgagagcca
      961 tcgttacagt gatagcatct tcaaaggctt cacgagtcaa gatatctgat ggtttgagac
     1021 caagttccaa catcttaaca acagcacgtc ctgctgcttc gatatcttct ttcttatcag
     1081 ctgattcagc tgggtgagag gatgaccctg gcaaactcat ccctagaact tcgatagcag
     1141 ttgccatggt attagcagta tacataccac cacaaccacc agggccaggg caggcattac
     1201 attcaagacg tttcacgtcc tcagctgtca tatcaccgtg gttccatttt ccgatacctt
     1261 caaagacaga aaccaagtcg atatctttac catcaagatt tcccggtgca atagttccac
     1321 cataggcgaa aatggctggg atatccatat tagcaatagc aatcatagat ccaggcatgt
     1381 tcttgtcaca gccaccgata gcgacgaagg catccacgtt gtgaccactc atagccgcct
     1441 cgatggagtc cgcgatgatg tcacgagatg ttagagagaa acgcatacca ggtgttccca
     1501 tagcgatccc gtccgctacg gtaatggttc caaactgtac aggccaagcg cctgcagatt
     1561 tgacaccttc tttagccagt ttcccgaaat catgcaagtg aatgttacat ggtgtatttt
     1621 ccgcccaagt cgaaatcact cccacaatcg gtgtttcaaa gtccttatct gtcataccag
     1681 tcgcacgaag catagcacgg ttaggtgatt taaccatgct gtcatagatg ctactgcggt
     1741 gacgtttatc taattcagtc atcttatccc tcccatttca gtttttacta ttatagcaca
     1801 atttaaaggc aatgaacaga ataaaattct tgaattttca gaaaattcta tacgcatgtg
     1861 aaatatttaa aattaaaaac aacaaagcgg attagtgcac tttctgatga ccagaatatg
     1921 ctttttaatc cgctttcttt aaataacgta atgtaatttt tacagaaatt ctttcagata
     1981 agtgtattta acatctatct tgcattataa atttctagaa ccttctcttt tatattcgat
     2041 tcactcaaac catactcatt aagaagataa tccattttcc ctacttgacc gaatctttct
     2101 tgaaccccca tccgatgaat ttttgttatt ccatcatcag agaataattc acataaagca
     2161 ctgccaattc cacctatctg attgtggttt tctacagtaa atatagtttt tccacttaac
     2221 attgttttta tctgttctgg tatcggtttg attctaaata aatctatcac acctactgag
     2281 taacctaatt tagacagttc atctgcaact cgaatacttg gagcaaccat tataccagaa
     2341 gcaacgatta caagatcttc cccgtgtctt aactcaatgt agcctttaga aaaatcttct
     2401 ccaccttgat acacaggaac tggagctttt ctaattgttc gaatatattt tagtcctttt
     2461 aagtctaatg tctggttcaa tatttcacga aattgaatat catcagttgc ttcaaaaatg
     2521 atcgatttag gaattaaacg taacaatcca atttcttcaa atggcatatg tgtcccacca
     2581 ttcatttctg cagttactcc tgcatctgat ccaatcacag tggcatccaa ttgtgcgtat
     2641 ccaagagaaa taaataattg atcaaatact cttcgtgaag caaaaggacc aaatgtatga
     2701 agataaggtc taaacccctg aatagacaag cctgctgcaa ggccgaccat ttctgcttcc
     2761 ataatcccaa cattcacata acggtctcca aagtcctttt caagattatg agtagccatc
     2821 gaacttgaca aatcggcttc taagactact atatcagaat cactttgatt agcctctaaa
     2881 aggaagtctc tatatacatg ccgtaattct ttcgtacttc tcatcattct gtttcctcca
     2941 attcctgact taatctttct acaactgaag ttaacatttg tttctcctct acagtagggc
     3001 gaagatgatg attggatttc atttcttcca gctcttgaac cccttgacct ttaatagtat
     3061 ctaatacaat gcacttaggt gatgaattat ttgactgttt taattggaca atcccttcat
     3121 aaatttctct aatatctgaa cccttgaccc taatggattc aaatccaaat gctgaaaatt
     3181 tttctacgaa atcacctgga ttacaaatat cctttgtaaa accatctaat tgttttttgt
     3241 tctcatcaac aaatacaatt aagttggata actgttgatg agaagcaaac tgtatagcct
     3301 cccaacattg tccttcattt aactcaccat ctccaacaat agcataagta taaaagggac
     3361 tctttcttat tctctgacca tatgcaagtc cagttgcaac actaattcct tgtcctaaag
     3421 agcccgttgt catatctatg cctggcgtta gatttctatc aggatgagac ggtaatttgg
     3481 ttccatttgt atttaaagaa tataagaatt ctttgtcaaa gaaaccattc aaatagagtg
     3541 tactgtatag agctggtcct ccatgacctt ttgataatat gaaataatct ctatctcgtg
     3601 ctgcaaatat ttctggagtc attggcatta tttcaccata aagcactgct aaaacttcta
     3661 cgatagacag acttcctccg taatgtccga atccaagatg attcaatgtt ctaagagtat
     3721 ttaatcggat gttagtcgca aattttctta actcatcttc tctattttta cttaaaatca
     3781 tcccttattc ctcctttgca gatggctttt taataaagga tactccaaac ataactgcta
     3841 aaataagaac aagaccaatc acaatgcctg cttgtgagcc aaattgattt aacattccta
     3901 aaataattcc tgatagacca aaatctgcat ctgagaaagt tgatccttgg aaaccgagtc
     3961 ctcccaaaac tggcattaaa aagactggga gaaaactgat taaaatacct tgtaaaaatg
     4021 ctccaatagt ggctccacga acaccaccag atgcattccc aataacacct gcagtcgctc
     4081 cacagaagaa atgaggcaca acgcctggta agataacaac cgttcctgaa gcaatcataa
     4141 ttgccatact tactaaacca ccaataaaac tagagataaa tccaattaga actgcattag
     4201 gtgcataagt ataaacaatc ggacaatcca aagcaggttt tgaattaggt acaagacgct
     4261 ctgaaatacc tttaaaggct ggaacaattt cgcccaaaat aaggcgaaca cctgctaaaa
     4321 taacaaatac ccctgctgca aattgacctg ctaattgtaa agcataaact agaccacttg
     4381 taccactact gatttctttt tctatatatt ctgaccctgc aaagatagct acaataatgt
     4441 aaataactgc catagataaa gtgatactaa cagtactatc acgtaaaaaa gctaaactct
     4501 ttggaaattt aatgtcctct gttgattttg atttgtcacc gataaggcta ccagtaaaac
     4561 cactcaacca atatcccaaa gaactgaaat gacctaaagc caccttgtca tttccagtta
     4621 attgaaccat atatttttgc acaaatgctg gggaaatact cataataata ccgagtgcta
     4681 atcctcctag taagatgaga ggcaagctag taaagccagc aactgataaa atgaccgcaa
     4741 tcatacatgc catatataga gtgtggtgcc ctgttaaaaa aatatattta aatcgagtaa
     4801 aacgagcgat taagatattg aacaccattc ctgcaaacat aatcattgca gtagctgagc
     4861 catatgttgt taaagctaca gctacaattg cttcattatt cggcacaacg ccagataaat
     4921 gaaaagcatg ctcaaacatg gtaccaaatg gatttaaaga attttgtaca attcctgcac
     4981 caccagatac aactaagaaa ccaacaaagg tcttaattcc acctttaata atatcaggta
     5041 atttcttctt ctgaagaact aatcctaaaa ttgcaattaa agctactaaa atagctggtg
     5101 tactaacaat atccaatatg aacttcatca tgacgctagc ctcctatata agtccttttt
     5161 cttcacaaag tttagtaatt aattctcgta gttcatccat atcaataata ctatttaaga
     5221 tacgaacatc tccaagatga ctagctgaat cagctagatc acgaccaaca atccaaatat
     5281 cagctgcatt tggatctgct ccacctaaat cataatgttc aacttctaca tccgaaacat
     5341 tcaaatcact caatacagat tcaatattca tctgtaccat aaaacttgaa cctaatcctg
     5401 aaccacaagc tgtaccaatt tttaacatta tctaatcctc ctgtttaatt atcattttaa
     5461 tatcattata gttttttgat gatattaaag tttgaacatg atttttatct cttaaaattg
     5521 ttgttaaatg tgacaaagcc tttaaatgac tctcattatc aatggctgca atacaaatca
     5581 acaatcttac ctcttgttct ggattatcca ataaataaat cggttcttcc aaaactaaca
     5641 ttgacattcc tatttcattc acaccttcat ctggccgagc gtgaggaatt gctactccct
     5701 tccctaaatt aataaaaggt ccaaactctt ctactttttg aatcattgcc tcagggtagt
     5761 tctcagttat cttatcttga tccaaaagcg gtttagctgc taaacgaatc gcctccttcc
     5821 atcctaattt ttgcgaacta acctgatagg tttctttggt aataagttgt tctagcactg
     5881 gtacaatttc ctttctatca tttttttggt aaagataatt ctttaacgcc aatcttaatt
     5941 ccaattcttg tgtaataatt ccatatcttt tgacaatatt caggatttgt tcaatctcaa
     6001 aatctccata ctctaaattc ggaaaatctt ttaacactag ttctactagt tgtattgctt
     6061 gctcttcagt catcataacc gaaactagat aatttggctt ttctgtctcc acctttatgg
     6121 tagaaaaaac catatcatag tcactactag ctttcacctg taaatcatca atctttgagg
     6181 ttcctataaa ctcaatttga ggaaataatg ctaatagatt ctcttttaac atcaatgaag
     6241 aactaacacc attaggacaa atgattgctg ctttatacca tttttgaggc aaagtatctg
     6301 ctttctttaa ataacctccg aaatggataa caaaatatgc tgtttcacta tcaggtatgg
     6361 gattgtcaat agcgtccatc aagggcatca aagaatcttt gactaattca aataaatcag
     6421 gataatgttc tttaacatgc aatacatatt catttgaact aggtaggccg aactttaatc
     6481 tatagtaagc cggtataagg tggcggcgaa gattttctct caatccttcc ctttgtttaa
     6541 aatgtaacaa agaaatatct tccattctac ttataatagc ctctgttaat tgattaaagt
     6601 aaaccggagc aacatctact tcaccttcaa agcaacttga taataaaacg gtgatatagc
     6661 gataatcatc ctcagaaaac accgtatcta taattcccaa atcaaccact gtatccaata
     6721 aaatagtggt tatatcttga ataacaggag atactaatgt ctctgaaaga catactcttt
     6781 caacatccct ttgataccta cacagaatga atactaaacc gaaaaggtaa acttttaatt
     6841 gattaacaat aggtactagc tgtagcttct cataataatc tttaactacc tgatcaatca
     6901 aatcataagt taatgaatac ccccaactgg ataaaacata atccaaaccc caaatcccta
     6961 tggaggattc cagcaactca ctaaccattt gaaaagctaa gcggtgctta ttccactctg
     7021 aaccgtgtaa agtataacct tttgctctac tgtaccctag ctccaaatca ttatctaaca
     7081 taatctttct taatgattga atatcagata aggttgtatt cttacttact ttcaaaaagt
     7141 cttggtaatg actattcgat ataaaatcta atcggcaaaa agtgtaaaga tagattaaag
     7201 ctaagcgagt cgactttggt aaaaccaatt catccgactt aataatatct gtcaaagact
     7261 gcttcgtacg atttgataaa ctatagcgac cttgcttttt atccagcact atccctttat
     7321 tagctagata aggcactaaa taatctattc cttctttgac ttcctttata ggtaagctca
     7381 ccttaacaga taattcatat aatgatagct cacaatgatc catcaaagtc atcaaaataa
     7441 ctagtgctct ataatcaaac atttctcttc c
//
LOCUS       DAVKGU010000088         7409 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKGU010000088 DAVKGU010000000
VERSION     DAVKGU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.089"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..304
                     /locus_tag="VW077_001757"
     CDS             <1..304
                     /locus_tag="VW077_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9455739.1"
                     /translation="VSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYCRIAED
                     AKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDDGQD"
     gene            288..890
                     /locus_tag="VW077_001758"
     CDS             288..890
                     /locus_tag="VW077_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9455740.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            892..1323
                     /locus_tag="VW077_001759"
     CDS             892..1323
                     /locus_tag="VW077_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455741.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            1385..2641
                     /locus_tag="VW077_001760"
     CDS             1385..2641
                     /locus_tag="VW077_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9455742.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            2649..3623
                     /locus_tag="VW077_001761"
     CDS             2649..3623
                     /locus_tag="VW077_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9455743.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            3623..4237
                     /gene="pmp23"
                     /locus_tag="VW077_001762"
     CDS             3623..4237
                     /gene="pmp23"
                     /locus_tag="VW077_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9455744.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            4311..5285
                     /locus_tag="VW077_001763"
     CDS             4311..5285
                     /locus_tag="VW077_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9455745.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            5359..6990
                     /gene="rlmD"
                     /locus_tag="VW077_001764"
     CDS             5359..6990
                     /gene="rlmD"
                     /locus_tag="VW077_001764"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9455746.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDIDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            7144..7293
                     /locus_tag="VW077_001765"
                     /pseudo
     CDS             7144..7293
                     /locus_tag="VW077_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            7273..7409
                     /locus_tag="VW077_001766"
                     /pseudo
     CDS             7273..7409
                     /locus_tag="VW077_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001909937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
BASE COUNT         2361 a         1418 c         1541 g         2089 t
ORIGIN      
        1 tgtatccaat ccaccctata tctctcgtga agatgagtca gaggtaggct tgaatgtttt
       61 gcattcggag cctcatctag ctctctttgc agacgaggat ggcctagcta tttactgtag
      121 aattgcggaa gatgcaaaag actatctcaa ggatggtggt aagatttacc ttgaaattgg
      181 atacaagcaa ggtcaaagtg ttcccgaact ttttagaaaa catcttcctg aaaaaaaagt
      241 tcgaacactc aaagaccaat ttggtcaaaa taggatggtt gtggttgatg atggacagga
      301 ttagacaaga gttggaaaat ggtggagctg tcgttctacc tacagagact gtttatggtc
      361 tttttgccaa ggccttagat gaaaaagcag ttgaccatgt ttaccaactc aaacgtcgtc
      421 ctagagataa ggcactcaat ctcaatatcg cctctttcga ggatatcttg cacttttcta
      481 agaatcagcc aacttatcta caaaaacttg tagagacctt tttgccaggt cccttgacca
      541 ttattctcga agccaatgac cgagttccct attgggtaaa ttctgacctt gcaactattg
      601 gatttcggat gcccagtcac cctatcacac tggatttaat tcgagagaca ggtcccttga
      661 ttgggccgtc tgccaatatc tcaggtcagg caagtggtgt aacctttgaa caaattctga
      721 aggattttga ccaagaggtt ctgggtctgg aagacgatgc ttttctaact ggacaggatt
      781 caactattgt ggatttgtct ggagacaagg tgaaaatctt acgccaaggc gcaattaaac
      841 gagaagatat tcttgctcgg ttgccagaga tttcttttga ggaggcttga aatgctaaga
      901 gatttgcaag aaacagatgt gaaagcgata tgtgacatca accaagaggc tttgggttat
      961 acttttagtc cagaggaaac ggctagccaa ctagctagac tgtctcagga ttcccatcat
     1021 ttcctacttg gctatgagga tgcagctaat catgtcttac ttggatatgt ccacgctgaa
     1081 gtttacgaat cactctattc caaagcagga tttaatatct tagctttagc agtttcacct
     1141 caagcgcaag gtcaaggtat cggtaaaagt ttactacaag ggttggaaca agaagccaaa
     1201 agatgtggtt atgggtttat ccgcttaaat tctgccaatc atcgtctggg tgctcatgca
     1261 ttttatgaaa aagttggcta tacttgtgat aaaatgcaga aacggtttat tcgcatcttt
     1321 tagtttgatt ttcttattgt aaaatcaaac taatggacta gtcacacaat aaaggagaag
     1381 acctatgatt tttgacaaag atgattttaa agcatatgat gctgatctct ggaatgctat
     1441 tgccaaagaa gaagaacgcc aacaaaataa tatcgagtta attgcttcgg aaaacgtagt
     1501 ttccaaggct gttatggcag ctcaagggtc tatcttgaca aataaatatg ccgagggtta
     1561 cccaggacgc cgttattatg gtggaactga tgtagtagac gttgtagaga ctcttgctat
     1621 tgaacgcgca aaagaaattt tcggtgctaa atttgccaat gtccaaccac attcaggaag
     1681 ccaagctaac tgtgcggctt acatgtcctt gattgagcca ggtgatacgg ttatgggaat
     1741 ggatttggca tcaggtggtc atttgactca tggggctcct gttagcttct ctggtcaaac
     1801 ctacaacttt gtttcttata gtgttgatcc tgaaacggaa ctcttagact ttgatgctat
     1861 cttgaaacaa gcccaagaag taaaaccaaa actgattgta gctggtgctt cagcctattc
     1921 tcaaattatt gacttttcaa aattccgtga aatcgcagat gctgtcggtg cgaagcttat
     1981 ggttgatatg gcccacatcg ctggcttggt tgcagctggt cttcacccaa gcccagtgcc
     2041 atacgctcat atcactacaa caacgaccca caaaaccctt cgtggacctc gtggtggttt
     2101 gattttgacc aatgacgaag aacttgctaa aaaaatcaat tcagctattt tcccaggtat
     2161 tcagggcggt cctttagagc atgttgtggc ggctaaggca gtttccttca aagaagtttt
     2221 ggatccagcc ttcaaggaat atgctgccaa tgtaattaag aacagcaagg ctatggcaga
     2281 tgtcttcttg caagaccctg atttccgtat tatttcaggt ggaactgaaa accatctctt
     2341 ccttgttgat gtgactaagg ttgtagaaaa cggaaaagtt gctcaaaact tgctggatga
     2401 agtcaatatt accctaaata aaaattcaat cccttacgaa accttgtcac cattcaagac
     2461 aagtgggatt cgtatcggag cagcagctat tactgcacgt ggatttggtg aagaagaaag
     2521 tcgcaaagtg gctgaactca tcattaaaac ccttaagaat tcagaaaatg aggctgtatt
     2581 agaagaagtg agaagtgcag tcaaagaatt gacagatgcc ttcccattat acgaggaata
     2641 aaacttttat ggacatttat attaagaaag ccattattca ccagttcagt ccggatgata
     2701 ccgagctgtt cttagcagat aagtttctca atattactcc aaaaatcgaa gaatacctac
     2761 gtaaaaaaat tgaacatgtg tattcagatg aagccaagac tgggattttc gaagaagaaa
     2821 atcccttctt caatcatatt acagacgatt tgttggagac atcagtaacg ctggctaatc
     2881 tctggaaaga ggagtttagc atttctgaaa atctcaagac caatgacttg atttttgttc
     2941 aattttctaa agaaggtgta gaacatttcg ctttcttgcg aattgccctg cgggagacct
     3001 tgacccacct cggaggagaa gttgataatc caatcaagct gactcagaat aacctgcctg
     3061 gatttggaac gggtgctgac gaggccttgg tggtcaatct tcagagtcgc aagtatcacc
     3121 tgattgaaaa acgaatcaag tacaacggga cttttttgaa ctatttttca gataatcttc
     3181 ttgctgtcgc tcctaagatt tctcctaaaa aatctatcaa ggaactggaa aaaacagccc
     3241 agagaattgc tgaatctttt aacacagatg attttcaatt tcaatccaag gtcaaatcag
     3301 ctattttcaa caacctagaa gaaagcaatg aattgtcacc tgagaaattg gctaatgacc
     3361 tttttgacaa caatctgacg gctcgtttga gctttattga ccaagtcaaa gaagccgtac
     3421 cagaacctgt tcaatttgat gaaattgatg ccagtcgcca attaaagaaa tttgaaaacc
     3481 aaaaactctc cttatcaaat ggaattgagc tcatcgttcc caataacgtc tatcaagacg
     3541 ccgagtctgt tgagtttatc caaaacgaaa atggaaccta ctctatctta atcaaaaata
     3601 tcgaggatat ccaaagtaaa taatgtttaa acgaattcga agagtgcttg tactagcagt
     3661 cttccttttt gctggctata aagcttaccg cgttcatcaa gatgtcaaac aagtcatgac
     3721 ctatcaaccc atggtgcgag aaatattgag tgaacaagac accccagcaa acgaagagct
     3781 tgtgcttgct atgatttata ctgaaacaaa aggaaaagaa ggcgatgtta tgcagtctag
     3841 tgagtctgca agtggttcca ccaacaccat caatgataat gcctctagca ttcggcaagg
     3901 cattcaaact ctgacaggca atctctatct ggcgcagaag aagggtgtag atatctggac
     3961 agctgttcaa gcctataatt ttggacctgc ctatatcgat tttatcgccc aaaatggcaa
     4021 ggaaaatacc ttggctctag ccaaacagta ctctcgtgag actgttgccc ccttgcttgg
     4081 taatacgact ggaaagactt atagttatat tcaccccatt tccatttttc acggtgctga
     4141 actctatgta aatggaggaa actattatta ttctagacag gtacgactta acctttacat
     4201 tatcaaatgt ttcactctct tttcaacatc tggctagacc aggtgttttt gttataagtt
     4261 ttctttaaga tagatatatt actctaaaga agtaaaggag gaaattccct atgagaaaga
     4321 aactctttct gactagcgct gcgatcttgt gggcagtaac agctatgaat agcgtccatg
     4381 cagcaacaga tgttcaaaaa gttatcgatg aaacctatgt ccaacctgaa tatgtcctag
     4441 gttcttccct atctgaagac caaaaaaatc aaactcttaa aaaactgggc tacaatgcct
     4501 caacagatac caaagaactc aagaccatga cacctgatgt ttattctaaa atcatgaatg
     4561 tggccaatga ctctagctta cagttgtatt catcagccaa gattcaaaag ctaggtgaca
     4621 aatcgccact tgaggtcaag attgaaacac ctgaaaacat cactaaggta actcaggata
     4681 tgtaccgaaa tgcagccgta actttgggtg tggaacacgc caaaatcact gtagcagcac
     4741 ccattccagt tacaggtgag agtgctttgg ctgggatcta ctattcgcta gaagctaatg
     4801 gagccaaggt gccgcaagcc aataaagatt tggctcaaga agagctaaag gctttgtcag
     4861 atatcaatgc tgaaaacaag gacaaatcag gctatgatgc taataaatta aacgttgccc
     4921 tagctgatat caagtcagga ctcgccaaag ctaaagaaag caagggaaat ctgacagaag
     4981 aagatatccg caagattgtt gaagatacct taaaaaatta caaacttgat caggtcataa
     5041 caggaaacca gatcaatatc atcatcaatt ttgccttgaa tctctcaaag agtgatatcc
     5101 tcagcaatgc agatttcact aaaaccctaa atgaccttaa acaaagcatc gtatcacaag
     5161 ctggcgacag ttttaaaaat atcaacctta actttgatgc ggataaggcg ctagaggacg
     5221 gtggtaactt cttaagctcc ctctggcaag cccttgtcaa cttcttcaag agttttggtt
     5281 cttaagaaat ttcatggtat aatggatggt aaccgtaacg ttgccgtctt ttttgtcggc
     5341 caatagaaag agaaaagaat gttaaagaaa aatgatattg tagaagttga aattgttgat
     5401 ttgacccatg aaggggcagg agttgccaag gtagatggtt tggtcttttt tgtagagaat
     5461 gctttaccga gtgaaaaaat tctcatgcgt gtcctcaagg tcaataaaaa gattggcttt
     5521 ggaaaagttg aaaaatacct tgtccagtca ccacaccgta atcaagatct agatttggct
     5581 tacctgcgtt caggaatcgc ggatttagga cacctttctt atccagaaca gctcaagttt
     5641 aaaaccaagc aagtcaagga cagtctctac aagattgctg gaattgcaga tgtagaagtt
     5701 gctgaaacgc ttggtatgga acatccagtc aagtatcgca ataaggcgca ggtgcccgtt
     5761 cgtcgagtga atggtgtctt ggaaacagga tttttccgta agaattcgca caacctcatg
     5821 ccccttgaag atttctttat ccaggatcct gtcattgacc aagtcgtagt agctcttcga
     5881 gacctgctcc gtcgttttga tttaaaacct tatgacgaaa aggaacagtc tggattgatt
     5941 cggaatcttg tggtgcgtcg tggtcactat tcaggacaaa tcatggtcgt tttggtgaca
     6001 actcgtccaa aagtttttcg tgttgaccaa ttgattgaac aagttatcaa gcagttccca
     6061 gagattgtgt ctgtcatgca aaatatcaac gaccagaata ccaatgcgat ttttggtaag
     6121 gagtggcgca ctctttatgg tcaagactat attacggacc agatgttggg aaatgacttc
     6181 caaatcgctg gcccagcctt ttaccaagtc aatactgaaa tggcggagaa actctatcaa
     6241 acagccattg actttgcaga gttaaaaaaa gatgacgtgg ttattgatgc ttattctggt
     6301 attggaacca ttggtttatc agtcgccaag catgtcaaag aagtctacgg tgttgaactg
     6361 attccagaag cggttgagaa tagtaaaaaa aatgctcagt tgaacaatat ttcaaacgcc
     6421 cactatgtct gtgacacagc tgaaaatgct atgaagaatt ggcttaaaga tgggattcaa
     6481 ccaaccgtta tcttggttga tcctccacgc aagggcttga cagaaagctt tatcaaagca
     6541 agcgcccaaa caggagccga tcgcatcgcc tatatctcct gcaatgtcgc aaccatggcg
     6601 cgtgatatca aactctacca agaattggga tatgaattga agaaagtcca gccggtggat
     6661 ctatttcctc aaacgcatca cgtcgagacg gtagcacttt tgtccaaact cgatatcgat
     6721 aagcacataa gtgttgaaat tgagctggat gagatgaatt tgacaagtgc ggagagcaaa
     6781 gcaacatatg ctcaaatcaa agaatatgtt tggaataaat ttgaattaaa agtttcgaca
     6841 ttatatattg cacagataaa aaagaaatgt ggaatagaat tacgagaaca ttacaacaag
     6901 tctaaaaagg ataaacaaat tattccacag tgtacacctg aaaaagaaga agccatcatg
     6961 gatgctttga gacacttcaa aatgatttaa tagaaaagaa tgacagtata tgactttctg
     7021 catttattac attcctactt ggtataggaa cggctattat tcctttcttg caaggtatca
     7081 attagaaaat aggctcaata taaagattga taggatcatt tttatattta aaggagtgtt
     7141 gaaatgattg ataaaggcaa caaaaaattt taggataaat ttgctaagtt gtatgcccct
     7201 tttatgaaaa aagataaaga ggtttatgat aaagtttgtg aatatcttag tcctcatttg
     7261 aataaagata tggaggtgct tgaacttgct tgttggtttc gtgtcataat agttatagag
     7321 gcaaatagtt atgtaaatat aaggagttca agacttctac caaagtttaa aactcaaaaa
     7381 ataaatagtt ggtgtgctgt ttatagtat
//
LOCUS       DAVKGU010000089         7239 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKGU010000089 DAVKGU010000000
VERSION     DAVKGU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.090"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            462..687
                     /locus_tag="VW077_001767"
                     /pseudo
     CDS             462..687
                     /locus_tag="VW077_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            854..1102
                     /locus_tag="VW077_001768"
     CDS             854..1102
                     /locus_tag="VW077_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9455747.1"
                     /translation="MQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHKNA
                     KTVNNIYTHVSKRMEQATLEVLNTISLNRKYIRSNLDK"
     gene            complement(1377..2882)
                     /gene="pyk"
                     /locus_tag="VW077_001769"
     CDS             complement(1377..2882)
                     /gene="pyk"
                     /locus_tag="VW077_001769"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9455748.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(2941..3948)
                     /gene="pfkA"
                     /locus_tag="VW077_001770"
     CDS             complement(2941..3948)
                     /gene="pfkA"
                     /locus_tag="VW077_001770"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9455749.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            complement(4030..7131)
                     /locus_tag="VW077_001771"
     CDS             complement(4030..7131)
                     /locus_tag="VW077_001771"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9455750.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGQSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
BASE COUNT         2196 a         1596 c         1281 g         2166 t
ORIGIN      
        1 aatcaactat aaaacaatat agactagtaa tggttggact caataagaat tgggtactaa
       61 gataggtata agtaaaaaca ccattagaaa ttatgaaaaa agggttaggt ctactaagaa
      121 aaatactata tttgacttag ttaaagtatt cagtagcttg attgatgccc tctttcctcc
      181 agttcaaaag gattctccta gcgatatcca atctatctac gaccaacggg caccacccag
      241 acaaggtaaa gttctgacct atgccgagag gcaactgtat gatcagaaaa acgaagtatc
      301 gtaaaaatca tcagactgga cgactacaga cagactactt actctcgtgt taggtagttt
      361 atctctgcag gtagccgctc gatgcaggac gacgatttag ttatggagtt tccattatcc
      421 cagacgacta cgacccaatc gcttatgtcg tcagcaagtc catggagcca aagataagaa
      481 acagcgacta cctatttatc aagaataccc cacagattga ctataatacc atcggcatct
      541 tccaagtaga cggcgctaac tatgtcaaga aactccgcca aggctacctc aaaaaaactc
      601 aatcccgaat accccgacat ccacctagac gaaagcaacc atattcgtac catcgggaaa
      661 gttgtcaggc tatataggga gaattaaaaa aggggggaga atggcgatat aaaacaaaca
      721 gattttaaaa gattcgaaaa actttgaaaa agttcattct acatttcaaa aaaagaataa
      781 gtgacctaaa gtagattaga ttttgttaaa caatagtgat atgtaaattt agagaaaggc
      841 atttaagatc actttgcaaa aggcaaatac taaactagag aaaccgataa accagcccct
      901 tagttcccat attttcaggc acacactcct tagtacacta gctgagaaga atgctataat
      961 ggttagagtg gaacacaaaa acgctaaaac agtaaataac atctacactc acgtaagtaa
     1021 aagaatggag caagctactt tggaggtttt aaataccata tcattaaatc gaaaatatat
     1081 cagatcgaat ctagacaagt agattgcgat tgctaaaaca tttgtagaaa ttcatttgac
     1141 tttctaatca atttgtgcat atcttatttc atactcaatg aaaatcaaag agcaaactag
     1201 gaagctagcc gcaagctgta cttgagtacg gtaaggcgac gctgacgtgg tttgaatttg
     1261 attttcgaag agtatcaatc cattatgaaa gttttgcccc ctttttgccc cctggacaaa
     1321 aaaagagccc atcatacagg ctagaaagct ggatatgata ggtttttata tttttcttaa
     1381 cgtactgtgc ggatacgcat tgtgtttgtg cgaacagctt ctcctactgg cacaccagca
     1441 acgataacga tatcatcgcc tgactcaacg agacctgctt ctaccgcttt acgttcagcg
     1501 atttcgaaca tatcgtcagt tgaagatgga gcatctgtca acattgggat aacaccccag
     1561 ttcaacatca agccacgttc tgtcaattcg tcaaatgtca atgctaagat gtcagcattt
     1621 ggacggtatt tagaaatcaa acgtgcagta tgacctgtct tagtaagagt tacaaccaat
     1681 ttgatatcca ttgagctagt agcatcttta acagcagaag ccattacttc tgtcttagag
     1741 ttacgctcaa atgaatctga atcaagacgt ccgtattcat taagaagagc ttgagcgttc
     1801 ttgtcgattg tagccattgt agttactgac tcgagtgggt atttaccgtt tgcagactcg
     1861 cctgacagca ttgtagcgtc agttccgtcg ataacagcgt tgaatacgtc tgatacttct
     1921 gaacgagttg cacgtggttt ttcagtcatt gtttcaagca tgtttgttgc agtgataaca
     1981 actttacctg cagcattgac tttcttgata atcatttttt gataaactgg aaccatttcg
     2041 aacggtactt cgatacccat atcaccacga gcaatcataa taccatcagc tgcttcgatg
     2101 atttcgtcta agttatcgat accttgttgg ttttcgattt tagcgaacaa ttgaacatgt
     2161 ccgtttccag tttcttcaca gattgcacga acttcgttca catcttttgc agtacgtacg
     2221 aatgaaattg cgatgaagtt gataccttgt tcaagaccga aacggatatc gtcgttatcg
     2281 cgttcagcaa gagctgggaa aggaatttta gtgttaggga tgttcacacc tttttgttta
     2341 gcgatgatac catcgttttc aacttcaact tcaaattcac gagttgcatc atctttagca
     2401 accacacgaa gaccaagttt accatcgtca accaaaactt gacgaccaac ttcaacatca
     2461 tcatagatat caagagcacc agcaacgttc aacgcaatca cttcacgagt tgatttgatt
     2521 ccttgtttag ttgcaacacg aattttttca ccagttttgt atgaatattc tttagcttca
     2581 ccttcgaaca attctgtacg gatttcaggt ccttttgtat caagaaggaa accaactttt
     2641 ttacctgcaa ttttttccgc aagtttaaca gttgccatac gctcaccttg ttcttggtgg
     2701 tcgccgtgtg agaagttgaa tcggaatgtg ttagcaccag cttcaatcaa tttagcaatg
     2761 tttttagctg aagcttcaac atcaagtttt tcaccccagt atccgtcctc accgaatttt
     2821 ttaccaccac ggatttctac cgcaggaccc aaagttgcaa cgatttttac acgtttgttc
     2881 atgatttttg tgactccttt atatatatga cctttttggt cttattaact aaattgtaaa
     2941 ttatgacaag ctcttattca agctagatag ctcaatatcg gctttgtgtg ggttgttaac
     3001 cacaatctta ccttctgcag taaggctaaa caatgcccct tcttctgcag taccaagaat
     3061 tggattttca accatttttt cgttacgaat accaaccgca acaccaccga taccttcttt
     3121 aagaagttta acagcatgtg cacccatacg tgacgccaaa acacggtcac gcgcagttgg
     3181 agaaccacca cgttgaatat gtccaagttc tgttacacga aggtcgcttg tatctccagc
     3241 ttctttaagt ttttgaccaa attcagccgc tgacatcaca ccttcagcta agacgatgat
     3301 attgtgtttt ttaccacatt cataaccagc tttgatgctt gctacgatat cttccatctt
     3361 gaagcctgct tcagggatga tgatttcatc agcaccagtt gcaataccag cccaaagagc
     3421 gatatcacca gcgttacgtc ccataacttc gattacaaaa gtacgacggt gacttgatga
     3481 tgtatcacga atcttatcga tagcgtccat ggcagtagta accgctgtgt caaaaccgat
     3541 tgtaaagtca gtaccaacga tatcgttatc gattgtacct ggaagaccaa tagctgggaa
     3601 gccatgttca gtcaaacgca tagcgccgtg gtaagatccg tcaccaccga taacaactac
     3661 accttcaatt ccgtgttttt tcaattgctc aatcccttta agttgccctt caagttgagc
     3721 gaactctggg taacgagctg agtgaaggaa agtaccacca cgagaaatga tgtcccctac
     3781 tgaagctgca tctaggggat gaatttcacc ggcaaccata ccagcatatc cgtcatagat
     3841 accaaaaact tccattcctt ctgaaattgc ttgacgaaca actgcacgga tggcagcgtt
     3901 cataccaggg gcgtctccac cactagtcaa aacagcaata cgtttcatat tggttatgct
     3961 cctttttctt ttaacattct tactgattat accacattta cgttgaaaat tcttctattt
     4021 tccgtatttt tagcgataaa tcgttttcat aacgatttca ttcaatttct cctctaattc
     4081 attggattta gctacaaaat gatggggaga aacgatggtt ttctgttcct cttcataccg
     4141 gatgatgact gggattgggc ctttaaattg ttctaaaatg cgtgaaattt cttgatccga
     4201 ttcatgattt ttcacctgta tccaaaagcg ttcagcaact gcttctctta tttcttgtgc
     4261 aatcatttgc agacggccat cacgtgattg tatttttcct tttacatagt agaaggctcc
     4321 ctcttttatt tcctgtccaa cctgacgata taagtctgaa aagagagtga catccaattt
     4381 tttcttacta tcatctgcct gtaagaaggc catattttca ccctttttgg tacgaatcac
     4441 ttttattttc tgaacttcaa ccaatataat agcatagcta ttttctgaca aatttccgat
     4501 tggggtaatc gggtaaatag ccttacttgc aatagcttgt agtggatgtt tgctgacacc
     4561 tatccctaaa agctcttgtt ccatataaaa tttttcttgt tccgtccaat cttccgattc
     4621 ctgccaacta taaatagcat ctccaaacaa acttcccaac tctttcacaa attcaaatag
     4681 attagctaag ttattaaata ctttttgacg atttttttca aatgaatcga aaagaccaac
     4741 ttttaccaaa ggttctagca gaggaagttt cagataattc tcaggtaatt tagctataaa
     4801 atcttcaatg ttagaataag gtctattttc aataatccag agagctaaat cattactgac
     4861 tcctttaatg gatttcaaac ctagatagat ggccttgttg gcaattttat cgtgataggg
     4921 aatggtgttg atggatagag aggctacttc aaaacctgct tcaagtgcat ctattaagta
     4981 atcactgttg gaagaattta acatgacctg ataaaaaatg gctggataat gcgttttgaa
     5041 ataagccaac tggaaggcca aggctgagta ggcataggcg tgtgacctgt taaaaccata
     5101 acctgcaaac ttctccataa catcaaagac ctgctctgct ttttccacag tatgaccagc
     5161 ttctaatgaa ccttgaataa aggaagccct catctcatgc atggcagagg catccttttt
     5221 ccccatagcc cgacgcaaaa tatcggcttt cccaagacta aatccggcaa gtcgctgggc
     5281 aacctgcata acctgctcct gatagagcat tatgccgtag gttggagcca aaatatcctc
     5341 cagtactgga tccagaacag tcacttcttc ctgcccatgc tttcttgcca caaaattatt
     5401 gatatagtca ctagcacccg gtcgatttag agaagtagtc gcgacgacat cttcaaaaca
     5461 gactggttgc acacgcttaa gcagacgaat ggcacctggt tgctcaaatt gaaagatacc
     5521 ttttgtatta ccagaggcaa ataaagctaa cgtttctttg tcttctaaat cgatttcttc
     5581 aattttcaga tgaatacctt ctgtttcagc aagcaactct tgcatcttct ggacaaaggt
     5641 caaatttcgt agtcccagaa agtccatctt caaaagtccg ctagcctcaa ctccatgagc
     5701 atcatactga gtcagtggaa tttcatcacc atactttaga ggaatgtagt tggttaaatc
     5761 ttggtcacta attacaacac cagccgcatg gacagaggtt tgccttggat agccctctat
     5821 cttgcaagca atctcaaaag ctttttggta ttctaactta ctattgattt gctgacgaaa
     5881 ctggagattg ccctcatagg ccgacttaag attgtcacga aaactgattt tcttagtaat
     5941 tgcagataat tcatactctg gcacaccaaa gcgtttcaag acatctcgaa gagcttgctt
     6001 ggctccaaag gttgaaaaag taacgatttg tgccgcatgt ttactaccat atttattacc
     6061 aacatatctg ataaaatctg gacgataaat atctgggata tcaatatcaa tatcaggcat
     6121 ggtatagcgt tcacgattaa gaaagcgttc aaaaatcaga tttttctcta ctgggtcaat
     6181 ccccgtgatg tctaaggcat aagaaaccaa actgcctact gcagaacccc ttcccattcc
     6241 catataatag ccattcgatt gtccaaaacg caacaaatcc caaacaacca agaaataatc
     6301 atcaaagccc atatcatgaa taacagacaa ttcttggtct agtctatctt gatattcttt
     6361 actagtcaac cccttctgaa caagccccag ttcagcacgc tctctcaact cctctactgc
     6421 tggtctagct ggattaaaac gaggcagttt cagactagta tccaagtcgt aagaaatgcc
     6481 tgaaataagc ttttctaaat tgtccaaagc ttgcggaaaa cgctcttgga atagtttctc
     6541 taaagaactt gctgatataa agacatcttg tctcgaacgc aagggaactt ctctgagcgg
     6601 tagattttct ttaatcgctg ttaaaacttg aagaacttct ctatccctgc tttcaaaagc
     6661 gttgacccga taaagaggta agataggatg atgaaattcg cttgctagtg tttctggata
     6721 aacccctata tagtaatcac agcctagttc taacgactca actctatcaa aataaggcac
     6781 aatgaccgcg atatcctcca ggtactggga caggactgac caagttttct ccccctgcat
     6841 cttggctgtc gaaagcttca tcaactgctg atagcccaca ctagatagag ctaaaaagcg
     6901 caaattcact ccctgatcat ctacaaacac tgtcatttca agccctagca aaggatgaat
     6961 gccgtatttt tttgtaatct ctagaaagtc gaaagcgcca taaagattgt caatatccat
     7021 catagccaaa tgagtgtagc cgtattcttt agctgctctc acatactttt cgatcgaaat
     7081 gacgctttcc ataaaactat agactgtttt tgtatctagt tgtgcgatca atttacactt
     7141 ctcctctatc cttctcacta tattatacca ttttcaccta taaatggctt ctcttgagaa
     7201 aaatttcatc agcttactaa tttattttct attcatttg
//
LOCUS       DAVKGU010000090         6842 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKGU010000090 DAVKGU010000000
VERSION     DAVKGU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.091"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            87..3437
                     /locus_tag="VW077_001772"
     CDS             87..3437
                     /locus_tag="VW077_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9455751.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(3943..4413)
                     /locus_tag="VW077_001773"
     CDS             complement(3943..4413)
                     /locus_tag="VW077_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9455752.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4430..6703)
                     /locus_tag="VW077_001774"
     CDS             complement(4430..6703)
                     /locus_tag="VW077_001774"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9455753.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2100 a         1150 c         1482 g         2110 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgaaaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tggagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttacatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgaattt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gaacaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctacgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctt tggagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgt atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tgtttaattc ctttattttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgacca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggataattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgttgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctctggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gcagatggaa
     3781 gctgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaaacg
     3841 catagtatca agggttttca acacttgata ctatgcgttt tctgatgtta aagactttct
     3901 accaggtttt ttaaaagcat aattgttagt tgtagtcatt tattattctt caaagaaaaa
     3961 tggtggggcg aattttttca gttcttcaaa gcacttttga gcagtatctg catcttcaca
     4021 gatgataaga cagacatcat taccacaaag ggtagcgata gcgtcaggga agctcaaagt
     4081 atcaatgata gaaccaaagg attgagccag tccaggaagg gtttttagta ggacttggtg
     4141 ttgaactggg cgcatccaga caagggcgtc ttccatgtag agttcgagac gtttttccca
     4201 ttttgagatg gaaccattgt taagaacata ataagcgcta tcttcttcgc ggacttttga
     4261 taggttcata tttttgatgt cgcgtgagag ggttgcctgg gttacttgaa tgtcgttctc
     4321 agcaagaagg gcttgcaact cagcctgtgt atgaatcttg ttttttgtga taagagcgcg
     4381 tataagttgg tggcggtgtt ctgatttatt cataataatg taactccttt tagcaaggta
     4441 aggtaagcat ggactgagcg aggtcgacag tcaagtggta gtctgtattg tcacggatgg
     4501 tgatttcaaa gtcagtagta tagaggacta aacggagagt gtctccttct tttagcttgt
     4561 aaatagttgg ctgcagttca aattgaacgt ccatccattc atctgcagta atatcctcta
     4621 ctaacagtaa atcatttcta ttttgtaaat taaggtaacc ttttgtcacg actcgttgtg
     4681 cctctggtct aaatggcaat tcacagagat tttccaacat gtgatagcga ccgttgtcaa
     4741 tggttctagc acttaaaata gctggataag gttgtaggta tttcttttgc ccaaattcta
     4801 gcagttgggc agataagagc cccttgtttg tactggattt gatacgaaga ttgagctgag
     4861 cgcgaccgtt taggtggaga tctttagtca caggaaggtt aatagtaatc tgattggctt
     4921 tcccttgata gagctctgta ttgaaggttt ggtatgtctt accatagcgc tcaaaatcct
     4981 tatctgggta ctggttttga atagcttgct cttcttgacc aagtgagaag gtttcacagt
     5041 tttcttgccc accgaagtta tcaagtgata accaagtctg tggagctgta ttgtcctgcc
     5101 agataacagt aggaagttga aagtctgttt cctgtcctag taatttcttg gtcaataagg
     5161 catttatgga ctcacggaag tcaattgatt gccaattgtt catgtaaaca tgggcaccat
     5221 tatggaaaaa gagatgcttg tgtatatgag taggaagagc atggaacatc tggtaaacat
     5281 gaagtggttt gacattccaa tcctgagaac catgagtaaa gacaacctct gcctttactt
     5341 tatgggcatt gagcagataa ttgcggtcat gccaaaactg attgtagtcc ccagtttttc
     5401 ggtctagctg agctttcact ttttctaagt cagcttggtg agcttcatta ccacggatat
     5461 agtcgccagc taagagatta cgagaatagg ttaactcagc aagggagtca aagtcctcac
     5521 ctggataacc acctgggcta gtcaccagac cgttttcacg gtagtagttg taccatgatg
     5581 aaattcctgc ctcggcaatg ataacttcta aaccatcgac tcctgtagtc gcaagaccat
     5641 tggacatggt acctagatag gaaagtcctg ttgtagcaac ttttccgttt gaccaatcag
     5701 ccttgacttg acgctggcgc gtgtgatcag taaaggcacg gcaacggccg ttaagccaat
     5761 cgatgacatt tttataagcc tcgatttgct ggtagtctcc attagtcatg aaacctgtcg
     5821 agtctttggt accaacacct gagacataga gattggcaaa gcctctcgga aggaagtagt
     5881 cgtttagtgt atagctagag ttgatgtgag ttagcttttc ctcagcctct gctataagct
     5941 cagctttacc ttggggttgg acgagattta gttgaggttt ctctagctca atcttgtgag
     6001 gaagcttaac ctcaagctcg ccctccatct tgtagagagc cttgtcacta gccttgtcat
     6061 tggttccctg atgataaggg ctggctgtca tgatggcagg gatttttcca tcaaaacgag
     6121 ggcgaataat gctaaccttt actaggtctg atagcccttt ttggtcagta tcgacacgag
     6181 actcaacgta aacgacttca cgaatgacat cctggttaga aaaagtagcc aaactcttgc
     6241 cgttaaagta gtggtagtca ttatcctccg gaataagacc atcactaaca agttggtcga
     6301 taagagtatt tccttttttg gtgcgagtat tgagtaactg atagagattt tcaatcaagt
     6361 caccatatat aatgggaaat ccagtttctt tacgaaaaac gtcactatct tcgaagtcaa
     6421 ccaaataaga aaagcctaaa agttgaaaag caacagtata aaaaatatct gctgtcagtt
     6481 catcttctga ttgaaaaaat gtcagcaggt ctgttttttt atcagctgct aggatagaaa
     6541 gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg taaaaaggtt tcaagtgagt
     6601 ctttgtgatt ggctgtattt tgtaaatcaa agccacattt ttttagttca gataagacat
     6661 tttcttttgg aaaattgata taactatatt gattaaaacg catagaacct ccatatagaa
     6721 tgacagttaa ggttattata tcaaaaaaaa agcagaaagg gaattgttaa cttcaaaagg
     6781 aaataatcca ataaaaatga ataaagtact aaattcaata tagagaacag agtaacaata
     6841 ag
//
LOCUS       DAVKGU010000091         6560 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKGU010000091 DAVKGU010000000
VERSION     DAVKGU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.092"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            327..686
                     /locus_tag="VW077_001775"
     CDS             327..686
                     /locus_tag="VW077_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455754.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSSFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            696..1061
                     /locus_tag="VW077_001776"
     CDS             696..1061
                     /locus_tag="VW077_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9455755.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIDKSHCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSRLISQEQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            1048..2898
                     /locus_tag="VW077_001777"
                     /pseudo
     CDS             1048..2898
                     /locus_tag="VW077_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3154..4573
                     /locus_tag="VW077_001778"
                     /pseudo
     CDS             3154..4573
                     /locus_tag="VW077_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886556.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4811..4945)
                     /locus_tag="VW077_001779"
                     /pseudo
     CDS             complement(4811..4945)
                     /locus_tag="VW077_001779"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(5266..6219)
                     /locus_tag="VW077_001780"
     CDS             complement(5266..6219)
                     /locus_tag="VW077_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU9455756.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASSNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT         2152 a         1126 c         1256 g         2026 t
ORIGIN      
        1 gattgttatg agagcttttg ctctcttttt tcttaccttc ctttcaataa tgtgatacct
       61 tttccaaatc atccctgttg tcctaatatc attcctaaga aaataaaacc tcaatttttc
      121 actgggggtt tgggggcgaa gccaccaagt tatcttatgg ttagctgtca aaactggaag
      181 gttttggtta gctggcgata tgattttttg ggtattgtga ccacaatgcc tgagctcgca
      241 aagaccgaac aaggacttta agaaatttcc cgtatgacac acggggtcgg ggtttatccg
      301 agaaaggatg aaagtgagga aaagagatgt ctgaacagta ccgtgacatt cgaaaagagg
      361 taaatctaac cgcagatgag ctaaaacaga ttgaaaaaat gatggaggtt gataattatc
      421 gtcacttttc atcattcgtc agggataaaa ttttgatgac tgatgacaaa cagttagctg
      481 ccaaagaatg gttttccctt tggcagtctc aaaagtttga acagatcagt cgagatgtgc
      541 atttggtttt gattatcgca agagagaatc accaagtgac tcaagaacac gtgtcaatct
      601 tattgacctg cgttcaagaa ttgattgcgg aagtcaatca agtacagtca ctcagtcgtg
      661 ggtttcgtga aaaatacatg agataggagg ctactatggt ttatcgttat cgtaccaatc
      721 tcaaaaaagt atttctaaca gattcagaat tacatcaatt gaatgagcga atcgataaga
      781 gtcactgtca aaatttttca gtctatgcca ggaaagtgtt gctgaatccc aatatgtcat
      841 ttgtcaccat taatacggat acctatgacc agttagtatt tgaattgaga cggatcggaa
      901 ataacattaa tcaaattgcg cgtgctatca atcaaagccg tctgatttct caggaacagt
      961 tacaagaatt gagtaaagga gtcggagagt taattaagga agtggacaaa gaatttcaag
     1021 tggaggtgaa aagactgaag gagtttcatg gtagttacta aacactttgc gacgcacgga
     1081 aaaaaatatc gtaggcgttt gattaaatat atcctcaatc cagataaaac agataatcta
     1141 aaattagtat ctgattttgg catgagtaat tacttagatt ttcctagcca tacagaaatg
     1201 gtagaaatgt acaatgttaa ctttactaat aacgacaagt tgtacgagtc cagaaatgac
     1261 cgacaagaaa aacaccaaca aactattcat gcccatcatc tcatccaatc attttctcct
     1321 gaagataatc tgacacctga agaaattaac cgcattggtt atgagaccat gatggaattg
     1381 acaggaggtc gttttaagtt tatcgtagca actcacacag acaaagatca tgttcataat
     1441 cacatcctaa tcaacgctat tgaccgcaat tcagataaaa agttgatatg gaactatgcc
     1501 ttggaacgaa acctccgtat gatttcagac cgtatttcta aaatggctgg tgcaaaaatt
     1561 attgagaaac gttattctta tcgaggttat aaaaaatata gggaatctag tcataagttt
     1621 gaattgaaac aacgtcttta ttttttgatg cagcaatcta aatagtttga tgatttttta
     1681 gagaaagcaa agcagttgca tgttcagatt gattttagtc agaagcatag tcgattcatg
     1741 atgacggatc gggcaatgac aaaaccaatt cgaggacgtc aactcagtaa acgagattta
     1801 tatgatgaag atttttttag aatgcatttt gccaagcaag agattgcaag tcgtttagaa
     1861 tttttgttga actgtgtcaa ttctttagaa gggttactaa caaaatcaaa agaattgaat
     1921 ctaaccattg acttaaaaca aaaaaatgta atctttatac ttgaagaaaa tggaaagcaa
     1981 ttcagtctga gtcataaaaa aataagtgat gagaaattat atgatgtcaa tttttttcaa
     2041 gattatttta aaaataagga agtaggtgtt tcagaaggaa tagaaaatct acaggcacag
     2101 taccgtgctt ttcaagaaga acgagataag gagaaggtat ctactgaaga aattgaggaa
     2161 gcctttgaga catttaagga aaaacgagat gccgttcatg aatttgaagt aaaacttaca
     2221 gaacaccaaa ttgagaagtt agttgatgag gggatttata tcaaagtgtc ctttggtata
     2281 aatcagagtg gtcttatttt cattcctaac tatcaactgg atattatgga agaagaaaat
     2341 cagaaaaaat ataaagtcta cattcgtgag acaacctcat attttgtcta taacaaagaa
     2401 cattcggata agaatcagta tatcaaaggg agaaccttga ttaggcagct aaccaacgat
     2461 agtcgagtaa taccatacag aagaccgact gttgaaagac ttcaagaaaa gatttctgag
     2521 attagcctct tgattgagtt aactgagaca gataagaaat accaagacat taaagacaac
     2581 ttggtttccg aaatagcaga gctagatata aaactgactc aaacaaatga aaaaattgcc
     2641 accttaaata agatggcgga agtacttatc aattctaaga gtgaaggttc gggtagccaa
     2701 aaactagcta ggcatgaatt ttctaaattg aacatgacag aatcaacaac tttagaacaa
     2761 gtcaatgaag aacttttgaa gttacaacag gaatttggta atgttcttga tgagtatgag
     2821 aaaacaataa gaaagcttgg acaattattt aaggtttttg atgagtgtat caataaagaa
     2881 attatgaatg aaatataaat cagactgaag acaaactcaa atttttttgg atatcttttc
     2941 tgtttatggt tgccaatatc ggtttaactt ctaaaacaaa cattaataaa aatgcattca
     3001 aagacgattt tacctatttt acgattacgc ttcgacccca aatcattaaa gttgtctatg
     3061 ctctatcatt gcatcactgg acttgtcaag agttaagata attcggagtc cactgaaaaa
     3121 gtcatcgtat gatggtatac taaagatgag gtgatggtat gcttgataat cgatgttagt
     3181 ccctattcta ctctgtgtga tattgttgtt cccaaaactc attttttacg acagctaatg
     3241 gaactctgtg attttagttt tatttatgac gagctggaaa agaattatca acctgacttc
     3301 ggatgtcgtt cctactctct ccttatcatg atgttcaagt atctcttatt aaaagacatc
     3361 tataagttgt ccgatgtgga tgtggtggag cgctcctttt ctggtatgac cttcaaatac
     3421 ttccttggtc tagctcctgt tattgaaccg tcctcattga ctaaattccg aaaacttcgg
     3481 aacaaagatg aacgattact tgatttgctc atcgctaaat ctgttcagat tgcgatagag
     3541 ctcggtctga ttaaaagtaa cattctcatt gtggatgcga ctcataccaa agttcattac
     3601 aatcacaaga aacctcaaga agtcctccga gaacgctcaa aagccttgcg taaaacgatt
     3661 tatcagtatt cggaatatat caaggcagaa tttcctagta agcctcagga agatactcta
     3721 gtagctgaat taagatatac acaagaatta atatcagtcc ttgagaagca tgacgaattg
     3781 actggaatac cagctatctc acaaaattca attaccttaa agaagctgta gaggatgact
     3841 tagagcactt ggaatcctct gtcaaagaag aagcaagaat tggacataag tcagccgata
     3901 gttcctttta cgggtacaaa gagcattttg ccatgaccga tgaacggatt attactgcct
     3961 gtgtggtgac atcgggtgaa aaaagtgatg gtcctgtttt agaagaactc tatcacaaat
     4021 ctaaggataa tggcgtgacg attgaagcta ttgttggaga tagggcctac tctgggaagg
     4081 ataatatgca atttaccaag aaagaacgtg tgcattgagt cgctaggcca tatccaacta
     4141 tttcaaaagg ctttcgtagg aaggaagatg agtttttcta taacaaggac gcaggtctct
     4201 ttgtttgtcc agaagggcat atggcactaa agaaggaaaa gacaggaaga aggacggctc
     4261 aggaaaccca ctattttgac atcaagcact gtcaaatatg ggctgttata aagaaggggc
     4321 taagtctaag acttatttga taccccttaa gagcgatgaa catattttcc agaagaggtt
     4381 tcaggaagcg ccctatttta aagaaaaggc caaacatcgc tacaagatag aagccaaaaa
     4441 tgcggaactt gaaaacagac atggtcttaa tgtagctaga gcaccaggtc ttttaaacat
     4501 ggagttacaa gccgccacaa ccatatttgt ggttaatatg aagaggatta taactctaat
     4561 aaaccaaaaa taaggaaaag caaagaggaa tctatccaag caggagatag atccctcttt
     4621 tttcatttta gttgaactgt agagcttact ttttcagtgg actcgatgat tagtgactat
     4681 ttttttcaaa ctctattagt tattggtacc ctaatgccga atggagatat ttaggattgt
     4741 aatagatttc aatatattta aagatttcgt tcaatagttg cgaaattggc atcattgaca
     4801 agttctcttt ttatgttttg taaaaggatt ccatgggtgc attatcatat ggattgcctt
     4861 ttcgactcag gctagacttg catcttttct gtttaaggag ttcttgaaat ctagctccag
     4921 tatattgaga tccttgatcg gtatggccga ctagttctta tttgagattt tctctatgat
     4981 aagcttgatt gaaaacattc gttaccaact tatcttgcat tcgatttccc attgcctaac
     5041 caacaatttt tctgctataa acatatatat aatgataaat aaagagtacc tgcttttgtc
     5101 ggaatataag ttaaatcccc cagccagatt tcatttttac cttttgtttg aaaacactga
     5161 ttaatcaaat tgggtcgtgt taatgaggaa tgccgacggc tatagtactt gtatttatag
     5221 cggattcctt tagcatagag acaaagccca aatttcaagt tgaagttagc cacccaaaag
     5281 aaattcactt ggcgacttgt agtccaagca ttttttaggg tagttgttaa tccaattttc
     5341 gataaaagct acttctttcg gagtcgtttt cttggttcct ttaggtaacc atctccgaat
     5401 taatcgatta tgattttcat ttgaacctct ctcccatgaa gagtaagcat gtgcgtagta
     5461 gatatgttcc tcaggaaata cctcagacaa tcgtttgaac tccgaaccat tatctgcagt
     5521 aatggacaga atacgatgct cccctaatag taacgtaagc gcctgattga cagattcagc
     5581 agttttattt ggaattaacc ttataatttg gtgtctgctc cgccggtcgg ttaagactaa
     5641 caggcaataa ttttttatct tagtcagtag gacggtatca atttcataat gaccattttc
     5701 caagcgaaga ttaataacgt caggacgttc ttcgatagat ttacctgccg gcttaaagtt
     5761 cgaactagct tgcttcttga cacctttcct ttttctggga taaagcatgt cggccttcgt
     5821 caatcctaaa tgaccattat gaaaccagta gtagatggtt gaaataccaa ctttcacttg
     5881 cttcttgtta accatcattt caggcgaaaa tttttgctta tgatagtgta agatcttctc
     5941 tctgatttcc tttgttaaaa ttaacttttt caccgaccgt tttcgattga attggtaaac
     6001 agtttgtgcg taatcggcag aatagacctt tttgtatagc ccttttctca cttgttgtaa
     6061 agttgtacct cttttgactt cattatgaat cgtttgaggc gcctttccta acaagcctgc
     6121 aatttcacga ttggacttat tttcattctt ccaccgctca atcaagcgac ggttatctat
     6181 tgtcaaatgt ttcccttttg gggtataatg ttcttgcatc tctgtgcctt tccttgtgtt
     6241 tgtggtgaac aacaagtata gcacagaggt gttttcttat accttaaatc acatttcatt
     6301 tgacaacagg aacccttgaa cctgtttttt cagctaaacc aaggctatga ttagccttgg
     6361 gcacctagcc taacagtttc aaacctgtta tcaaatgaaa tcatcaactt actcaaacat
     6421 agtgaaacat ctaattcttg actaaggtaa ggtggctaac ttcattatag aactttcaaa
     6481 tttatttatt cctgaggata atcatatatc tttattattg aaatcgcttg caaatcatgt
     6541 tactttgtgg taaaattata
//
LOCUS       DAVKGU010000092         6527 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKGU010000092 DAVKGU010000000
VERSION     DAVKGU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.093"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            88..491
                     /locus_tag="VW077_001781"
                     /pseudo
     CDS             88..491
                     /locus_tag="VW077_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            587..1351
                     /locus_tag="VW077_001782"
     CDS             587..1351
                     /locus_tag="VW077_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455757.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVATLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVTYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            1351..2103
                     /locus_tag="VW077_001783"
     CDS             1351..2103
                     /locus_tag="VW077_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455758.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLGEFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            2113..2811
                     /locus_tag="VW077_001784"
     CDS             2113..2811
                     /locus_tag="VW077_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455759.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLANWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            2808..3455
                     /locus_tag="VW077_001785"
     CDS             2808..3455
                     /locus_tag="VW077_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455760.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRELSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(3535..4400)
                     /locus_tag="VW077_001786"
                     /pseudo
     CDS             complement(3535..4400)
                     /locus_tag="VW077_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(4412..5169)
                     /locus_tag="VW077_001787"
                     /pseudo
     CDS             complement(4412..5169)
                     /locus_tag="VW077_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(5170..5457)
                     /locus_tag="VW077_001788"
     CDS             complement(5170..5457)
                     /locus_tag="VW077_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9455761.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            complement(5467..5847)
                     /locus_tag="VW077_001789"
     CDS             complement(5467..5847)
                     /locus_tag="VW077_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU9455762.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESE"
     gene            complement(5807..6487)
                     /locus_tag="VW077_001790"
     CDS             complement(5807..6487)
                     /locus_tag="VW077_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9455763.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
BASE COUNT         1886 a         1060 c         1368 g         2213 t
ORIGIN      
        1 tttcttttat tgatataatg aaaatgtaat atgtatcaaa ttgatcaatc aactatcttg
       61 tttatcactt cttaagagga cgatgtcatg aaaagaagaa aaatatattc cttttttgcg
      121 tagttacaat tgcatctagt ttgtttctcc ctacagtttt agctagacag attggaaatt
      181 atgatttaac gttgccgcgt tggggttcgg atacaactag tgagcttgag aaagaaaacg
      241 cctctgctgg aattaataat agtgacagca ctggtggcgg taaaaggtta agtacctcta
      301 ttcgtagcgc ctatagtggg tcagatatta ccccggtata ttcattgggg tctggctcta
      361 ggattgtcat gtactataat ggaggtggtg acaattatat tggttctggt actagattag
      421 ctatggcgcc acaatttgga aatcatgtaa gaattcatac ttcaggttct tggagtccag
      481 attcttatta acttacttgt cagagtaagc cttaaagatg gttgattgtg ggtgtagcat
      541 gaaaaaagaa tgctacaccc tatttttatt ataaggagga gtaaggatgg aatttttcat
      601 ttgtaatctt gtacgagtcg ttcaatcacc tcgattttat atgtctttat ttttgaccct
      661 tctttgcatg agtttaggaa atttccttgc tttcaatggt atttataaaa ttgaaggttt
      721 atcgattttt tttgccgctt cttctattcg aggattttca ccgattagcc tagtagctgc
      781 acttatctgt acactgccct attctagtca gataatagag gatgctgaga gtcattttct
      841 aacagcacaa ttgtgccgaa tttctaaaaa gaggtatcta gctattgtag gtagtactgc
      901 aattatttct tcttttctag tcttttttct cccctattta ttattattag gaattaatct
      961 tttagtgact ccttatcagg aaatttatat tggagattat agtggtgcct taaaagaatt
     1021 atttgattct aatcagttgc tctatagtct tgtggcgact ctttggtatg gagtttgggg
     1081 cgctgtgttc tctatttttg gactagctag tgctttgcta gtgaagaaaa aaataggagc
     1141 tattttcatc ccagttacct atatgatggt tggtggtatt ttttgggcta ttttagggct
     1201 atcttactta gaacctgtga caacattagc tttgggatat cagaaagata tcagtctttc
     1261 cttagttagt gggcatcttg cttttatttt atttgttagt tgtttggttg tttatggtac
     1321 attttttcta cattcagagg actatgtata atgaaacaat ttgttcaatt ttataaaaaa
     1381 gatttcttag cagtattggt ttattttata ttactgctat cctgtgtttt atctagtaca
     1441 gtatatttat tgcgctgtcg ccaatattca atccatccaa atgtattaga atggatttta
     1501 gttttacttc aagatatgac gactggagta tattgctttc cgttcacata tatattgttc
     1561 tttttttatt tgatgaataa ctattttaat aggttggagt gtcgcattcg tctgaaatca
     1621 attaagcact ttaccagttt tagtttcaaa ttagcagctc ttagtacggg gatttggacg
     1681 gcgactttat ttttattgat ttttctaatt gcatttagta atggttttag cttctctttg
     1741 gagataaagg aggttgattt tttaggagaa ttttatggta taagtattgc aaacaatgct
     1801 agtttcttta taggattttt tttctcttat atagcatact atttcttttt atccttactt
     1861 actattagca gtttttcttg gtttaaaaaa tcaaacatga gcttagtatt tctgtttact
     1921 tttttatttg tagaatcctt attctggatt tatcagttga acaatgggat aattggatta
     1981 ttgccaattt ttcagtatat ggtaaattcc aatccgtatg cattgattta ttggcttaca
     2041 ttactatcta tcataattcc attgactgta ttttctgttc atagaaactg gaggagagtg
     2101 taaaagttgg aaatgggaaa gttaagtagt cacatgtgga ggttgaatca gataatctat
     2161 accaagtact tttggggtta tgttcttttt tggatattga tttgtttagg attatggtat
     2221 tggttagaag gaaatgatag acttgttata gaaattttaa aagggcctaa tctgagtcaa
     2281 aactcttttt tagtcttatc tatatggttg cttcattggt ttattattca tacatttttt
     2341 ctagcagttg tatatcgtag aagagcatcc gatttcttta tggaagtgat tcgattttct
     2401 tctattaagc tctggattag gtatcagatt tggacctgtt ttctttatgg actcatttta
     2461 atcatggtaa aagttctagt gattcaattt atgttacagt tagcaaactg ggatatagga
     2521 gttttgttta tagttgattc tttgaatgct tgtgtgctat ccttgctttg ctttatgtta
     2581 tacgcactag gagcgaatgt acaaatgaac tttgcttgcg ttagtttctt tttactcatg
     2641 attgtgtttg gtggtctatt tgtaggaaat cgaactaatt atctatttta tattttgaat
     2701 cgagggaatg gagatatagg acgagattta tttcttcagt tgctattttt agtctttctt
     2761 tttaaaagca ttttctattt cactcgtcaa aagaggaggt ttatagaatg aatgaaataa
     2821 ttacattaaa aaatattgag ttgaaattaa aaaaaacatg tgtttttcaa aaccttaatt
     2881 ttagttgtaa acagggggaa attataggaa ttactggtgc gaatggctca ggtaaaagtg
     2941 tattgtttaa attaatagct ggtttatata gtccgtctta tggagaagtg ttaatcaatg
     3001 gggaaaatat tgttcctgag agaaaaattc cagctaattt gggagctttg attgaagaac
     3061 ctggttttat aaattattat agtggcttta agaatttaca atatttggca agcatacgag
     3121 gagtagttgg taatcaggaa atcaatgata cactgaaaat agttggtcta tatgagcaaa
     3181 aagaccagaa agttaaaact tattcgctag gtatgaggaa aaagctaggg attgctcaag
     3241 caattatgga gaatccctct attcttttac tagatgaacc tatgaatgcc ttggataaat
     3301 caagtgtaga aaatatgaga acattgttta gagagctctc tagtgaaaaa gggacaacaa
     3361 ttttgattgc tagtcatagt gaagaggata ttcgtatctt atgtgataaa gtatatgcaa
     3421 tagaagataa agtatgtaca ctgtgttcag attgaacatc ttagatagac tcaggatgtt
     3481 ataggtaaaa tcttaaaaag ggattggagc ttaaaatcca gtccctttta agagttaatg
     3541 gccaccttca acaaccaggt catctgcttt agcagcgtca ccgtaggttg ggtgaagtgt
     3601 tttttcatag gccttgtgga agaagttttc cttgcctaat ttttcaatat ctttattgat
     3661 gaagtctagc aattcttggt tgtctttttg aactgctgcc gcaatggtat cgggatcacc
     3721 gagggaagta attcctactt caaatccttt attttcaagc gcccaagcta gaacttccgt
     3781 attgtcagtt gaaaaggcat ctccacgtcc gtcaagaaga gcttggtaag agtcactgta
     3841 ttggtcgtat ttttggagtt tgatttctgg atgattcttt tcaaaataag tctcagcagt
     3901 cgttcctttt gtgacaatta aggttttacc ttcaagttgt ttgacgtctg taatgagacc
     3961 agtcttaggt gatacgacac ccagagaaac tttcatatat ggaagggcaa aatcaacttg
     4021 tttcttacgt tcgtcagtta ctgtaaagtt agcaagagta atatctacct tgtttgaaat
     4081 caagtattcc gcacggttgg cggcatcgac tgaaatgtat ttaaccttga caccaaggtc
     4141 ttgagctagt tggttcccta gttcaatatc gtagcgtagc cttggtaaga accatcattg
     4201 tcaacgtagc caaacggttt tttatctcca aacacggcga ttcgcagttc accgcttttt
     4261 ttgatttcat cgatagtgcg agctttggca gttgtttttc cagaggatga accagcgttt
     4321 ccacctgagc tacaagcagt gataaagata agggcaaagg caagtgctaa aacagttaag
     4381 agtggtttga atagtttcat aagaatctcc tttatagata tgagccgaat tggctaaagt
     4441 caaagacgtt taaaaattcc tgggctcgtt tggtttgcag attggtaaag aaggcttgag
     4501 ctgttccttc ttcagcgatt ttcccttggt cgaggaagat aatccggtca gtaatggctt
     4561 gggcaaactg catttcgtgg gttactaaaa tcatggtacg gccttcttgg gccaaatcat
     4621 tgataagttc cagcacctca cgcaccattt ctggatccag cgaagcagtc acctcgtcaa
     4681 aaaggatgat ttctggatgc attaggaggg cacggacaat tgcaacacgt tgcttctgtc
     4741 caccagataa ttgacgggca aagctatgtt gtttatccag caaaccgaca cgctctagta
     4801 attgcaaagc ttcttccgtt acttctttct tgtcccttcc ttgagctttg atagggccta
     4861 ggatgaggtt ttgtaagaca tccagatggg gaaagagttc ataactttga aagaccatgc
     4921 caatcttttg gcgaactagg tgaaaatctt ttttattttc aacgatagac tgaccatcca
     4981 gaagaatatc tccaccttga atactttcta agccgttgag gcaacgaagg agggtacttt
     5041 tcccacaacc agatggccct aggatgacaa caacttccct tttttgattt ctagagaaag
     5101 tccttggagg atgggattgt ctccgaagga tttttttagt tccttgattt ctaagatagt
     5161 ttcagacatt tagtttctcc aatgtttttc taagtgagtg gatagtttgg aaatagggta
     5221 gcaaactgcg aaatataaga ctagaatggt tccataaatc caaaatgaag cagttgggat
     5281 ggtcaggcga ttgctatcga tgatttgttg tccaactttg gtcacttcca caaccccaat
     5341 caaaacaact aatgaagtgg ttttaatcat ccgagtgaca agattgatag cctgcggtag
     5401 cagtcttctt aagacttgtg ggatgatgat gtggtagtaa agttgaacat tagtcaagcc
     5461 gagtgcctat tcactttcaa actgatgttt agggagagaa gtgatagctc cacgtaccaa
     5521 gtctcccatt tcagctgttc cccagagggt aaaaatgata atagctgaag tctcacctga
     5581 gatattgata ttaaagtttc gagccaagcc aaagtaaacg atgaagagta gcaccagctg
     5641 gggcatgata cggataaatt ccagatacaa tcgtgttaaa aatcgtatga ttctagaatg
     5701 ggaggtcatg atgattccca tgactgttcc gaacatcatg gataagagga cagacaggat
     5761 ggatatccca atcgtaacgc ccaacccctg taagattctc aggagattat ttccttgaaa
     5821 gagtacttgg attcccgaat cctgcatggc gggtcctcct ttctatccag ctaaagacca
     5881 gtgagatggg tagcagcatg atcagataag caactaccaa catagctagc gcaatgtctg
     5941 tctcatagta gagaccaatc aaatccttgg cgacgtacat gaggtcggcc aaagccactg
     6001 ctgagaaaac agaggtttcc ttgataagga aaatgacatt ggcactaaag gaggggagtg
     6061 ccaccgctgt tgcttgcgga agaaccacat agcgaaagac ctgtagaggt gtcagaccaa
     6121 tagcgaggcc aatctcttgc tgggtttgac tgatggcttc cagcccactt cggaaagatt
     6181 ctgccatata ggagcctcct aaaaagacaa gcccaagcgt tgcacagact tctgaagata
     6241 ggacaatccc gattcgggga agaccgaagt agagaaagaa gagttgaatc aaaaggggcg
     6301 tattacgtga caattcaatg taggctgtcg ctacttgcgc caaaacaggg attcgataat
     6361 gtcggatgat actaacgatt aaaccgagca gaaaggatcc caaaattccc caaactgcaa
     6421 tatgcaaggt cagaaagaat gccttttgat atagtggtag atattgttca acaatggacc
     6481 aatccaaaaa tagaacctcc catctagaaa taatacagtt attgtag
//
LOCUS       DAVKGU010000093         6416 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKGU010000093 DAVKGU010000000
VERSION     DAVKGU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.094"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(93..389)
                     /locus_tag="VW077_001791"
     CDS             complement(93..389)
                     /locus_tag="VW077_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9455764.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(379..540)
                     /locus_tag="VW077_001792"
     CDS             complement(379..540)
                     /locus_tag="VW077_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU9455765.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            610..1374
                     /locus_tag="VW077_001793"
     CDS             610..1374
                     /locus_tag="VW077_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9455766.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(1614..1766)
                     /locus_tag="VW077_001794"
     CDS             complement(1614..1766)
                     /locus_tag="VW077_001794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455767.1"
                     /translation="MKIKASIRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NISTAR"
     gene            1984..3363
                     /locus_tag="VW077_001795"
     CDS             1984..3363
                     /locus_tag="VW077_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9455768.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            3377..4042
                     /locus_tag="VW077_001796"
     CDS             3377..4042
                     /locus_tag="VW077_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9455769.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            4177..4251
                     /locus_tag="VW077_001797"
                     /pseudo
     CDS             4177..4251
                     /locus_tag="VW077_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4397..4936
                     /locus_tag="VW077_001798"
     CDS             4397..4936
                     /locus_tag="VW077_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9455770.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            5036..5413
                     /locus_tag="VW077_001799"
     CDS             5036..5413
                     /locus_tag="VW077_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9455771.1"
                     /translation="MIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLDLSQVSHPKMRE
                     YLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPKVFQAIRKTMLSRLTKH
                     SVLPDRKLSNVVYQITKSVYGFN"
     gene            5529..>6416
                     /gene="pavB"
                     /locus_tag="VW077_001800"
     CDS             5529..>6416
                     /gene="pavB"
                     /locus_tag="VW077_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9455772.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADG
                     LNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLV"
BASE COUNT         1733 a         1224 c         1485 g         1974 t
ORIGIN      
        1 gcctgcggct aacttcctag tttgcacttt aattttcatt gagtattact tcatcttctc
       61 atgaaattga gtttttgccc agctcctctt gattattcta cttgaccggg ccaagcattc
      121 ataccacctt ctacgttggt aacggtgagg ccttgggagc tgagaaattg gcaggtagat
      181 gcagagcgga ctcctccttg acaaatgaca tagtattcat ggtcaggttt gagttctttg
      241 tagccttgct ctaaggtact taatggaaga tttttggcac ctggtgcatg tcctgcttgg
      301 aattcatgtg cttcacggac atcgataagt tctagatttt cattttggta ttttccataa
      361 aagtcagcca tgctgatact agtttccata ttctactcct tctgggttaa ccattttgta
      421 tagtgaataa gcgccgtcaa ggttttgggc ggtaaatcct gcttgcttga ggataggctc
      481 tgcgatatag ctgcgcaaac cactgtggca gctgacgatg taggcttgtt ttttgtccag
      541 ttcatcagct tcctccttga cactttgtca gtttttgata caatagtaca aaattagagg
      601 aggcaggcta tgattcagaa acatgcgatt cctattttag agtttgatga caatcctcag
      661 gcggttatca tgcccaatca cgaggggctg gacttgcagt tgccaaagaa gtgtgtttat
      721 gcatttttag gtgaggagat tgaccgctat gcaagggaag taggggcgga ttgtgtcggc
      781 gaattcgttt ctgctaccaa gacctatcca gtctatgtca tcaactacaa gggtgaggag
      841 gtctgtctgg ctcaggctcc tgttggctcc gctccagcag cccagtttat ggattggttg
      901 attggctatg gtgtggagca gattatctct actgggacct gtggtgtcct agctgatata
      961 gaggaaaatg cctttctagt ccctgttcgc gctttgcgag atgagggagc cagttaccac
     1021 tatgtggcac cttgtcgtta tatggaaatg cagccagagg ctattgctgc tattgaggaa
     1081 gttttggaag acagagggat tccttatgaa gaagtcatga cctggacgac agacggtttt
     1141 taccgagaaa cggctgaaaa ggtggcttat cgtaaggaag aaggctgtgc tgttgtggag
     1201 atggagtgtt ctgctcttgc ggcagtagct caattgcgtg gggttctctg gggtgaattg
     1261 ttgttcacag cagattctct agcggacttg gaccagtacg acagtcgtga ctggggctcg
     1321 gaagctttta ataaggcgct agaactgagt ttagcaagtg ttcaccacct ttagttgtac
     1381 tggcaaagga tttgttttat cataaaatgt ctagctcata cttttcaaaa atatgtttaa
     1441 acgaggtcac cttcctcttg tcctaggcat gttgaggttg ggaaaaatct ttaaaatcag
     1501 aaaaacgtat catatcaggt gatgaaaact ttgatactat gcgttttatg ttggtaagat
     1561 ttagagtgag atgaaatgat actcttcgaa aatcttttca aaccaggtca gcttcacctt
     1621 gccgtagata tgttactgac ttcgtcagtt ctatctgcaa cctcaaaaca gtgttttgag
     1681 ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgagcaacc tgtgactagc
     1741 tttctaatcg atgccttgat tttcattgcc tataatcaaa aagagaaatt ttctcctgaa
     1801 aagcatatag agtagctggc gttaaaagct cctgtcttgc ttttttgacc tatagtcaca
     1861 tctatcaagt attgttcttg cctaagctat caataaaaag gtggcatttt ttaggcttgg
     1921 tgttagtgaa ttttgcctta tcctatctaa gtcatttcga gctttttatg gtacaatgga
     1981 aacatgttat tcaaattatc taaggaaaaa atagagctag gcttatctcg tttatcgcca
     2041 gcccgtcgta tttttttgag ttttgccttg gtcattttac taggctctct tcttttgagc
     2101 ttgccctttg tccaagttga aagctcacga gcgacttatt ttgatcatct tttcactgct
     2161 gtctctgcag tctgtgtgac aggtctctca acccttccag tagctcacac ctataatatc
     2221 tggggccaaa taatctgttt gctcttgatt cagatcggtg gtctagggct catgactttt
     2281 attggggttt tctatatcca gagcaagcaa aagcttagtc ttcgtagccg tgcaactatt
     2341 caggatagtt ttagttatgg agaaactcga tctttgagaa agtttgtcta ttctattttt
     2401 ctcacgacct ttttggttga gagcttggga gctattttgc ttagttttcg ccttattcct
     2461 caacttggct ggggacgtgg tctttttagt tccatttttc tagcgatctc agccttctgt
     2521 aatgccggtt ttgataattt agggagcacc agtttatttg cttttcagac cgatttactg
     2581 gtcaatctgg tgattgcagg cttgattatt acaggcggcc ttggttttat ggtctggttt
     2641 gatttggctg gtcatgtagg aagaaagaaa aaaggacgtc tgcactttca tacgaagctt
     2701 gtactattat tgactatagg tttgttgtta tttggaacag caactactct ctttcttgag
     2761 tggaacaatg ctggaacgat tggcaatctc cctgttgccg ataaggtttt agttagcttt
     2821 tttcaaacag tgacgatgcg aacagctggc ttttctacga tagattatac tcaggctcat
     2881 cctgtgactc ttttgattta tatcttacag atgtttctag gtggggcacc tggaggaaca
     2941 gctgggggac tcaagattac gacatttttt gtcctcttgg tctttgcacg aagtgagctt
     3001 ctaggcttgc ctcatgccaa tgttgcgaga cgaacgatcg cgccgcgaac ggttcaaaaa
     3061 tcctttagtg tctttattat ctttttgatg agcttcttga taggattgat tctgctaggg
     3121 ataacagcca aaggcaatcc tccctttatc cacctcgtat ttgaaaccat ttcagctctt
     3181 agtacagttg gtgtaacggc aaatctgact cctgaccttg ggaaattggc tctcagtgtt
     3241 atcatgccac ttatgtttat gggacgaatt ggtcccttga ccttgtttgt tagcttggca
     3301 gattaccatc cagaaaagaa agatatgatt cactatatga aagcagatat tagtattggt
     3361 taagaaagga aagagcatgt cagatcgtac gattggaatt ttgggcttgg gaatttttgg
     3421 gagcagtgtc ctagctgccc tagccaagca ggatatgaat attatcgcta ttgatgacca
     3481 cgcagagcgc atcaatcagt ttgagccagt tttggcgcgt ggagtgattg gtgacatcac
     3541 agatgaagaa ttattgagat cagcagggat tgatacctgc gataccgttg tagtcgcgac
     3601 aggtgaaaat ctggagtcga gtgtgcttgc ggttatgcac tgtaagagtt tgggggtacc
     3661 gactgttatt gctaaggtca aaagtcagac cgctaagaaa gtgctagaaa agattggagc
     3721 tgactcggtt atctcgccag agtatgaaat ggggcagtct ctagcacaga ccattctttt
     3781 ccataatagt gttgatgtct ttcagttgga taaaaatgtg tctatcgtgg agatgaaaat
     3841 tcctcagtct tgggcaggtc aaagtctgag taaattagac ctccgtggca aatacaatct
     3901 gaatattttg ggtttccgag agcaggaaaa ttccccattg gatgttgaat ttggaccaga
     3961 tgacctcttg aaagcagata cctatatttt ggcagtcatc aacaaccagt atttggatac
     4021 cctagtagca ttgaattcgt aaagagggat gacccctctt ttttgatgcc caagatggca
     4081 aatagagaca gaagcccctt gtcttctagt aaaagttctt caaaggctgg actttatggt
     4141 aaaatagaaa gaagtgacaa gagagagtaa tactcaatga aaatcaaaga tcaaactagg
     4201 aaactagcta cgggctgctc aaaacagtgt tttgaggttg tggatagaac tgacgaagtc
     4261 agctcaaaac actgttttga ggttgcagat agaactgacg aagtcagtaa catctatacg
     4321 gcaaggcgac gttgacgcgg tttgaagaga ttttcgaaga gtataagaaa aaatcagtcc
     4381 cctaaaggag cagattatga agttattgtc tatcgccatt tctagctata atgcagcagc
     4441 ctatcttcat tactgtgtgg agtcgctagt gattggtggt gagcaagttg ggattttgat
     4501 tatcaatgac gggtctcagg atcagactca ggaaatcgct gagtgtttag ctagcaagta
     4561 tcctaatatc gttagagcca tctatcagga aaataaaggc catggcggtg cggtcaatcg
     4621 tggcttggca gaggcttctg ggcgctattt taaagtagtt gacagtgatg actgggtgga
     4681 tcctcgtgcc tacttgaaaa ttcttgaaac cttgcaggaa cttgagagca aaggtcaaga
     4741 ggtggatgtc tttgtgacca attttgtcta tgaaaaggaa gggcagtctc gtaagaagag
     4801 tatgagttac gattcagtct tgcctgttcg gcagattttt ggctgggacc aggtcggaaa
     4861 tttctccaaa ggccagtata tcatgatgca ctcgctgatt tatcggacag atttgttgcg
     4921 tgctagccag ttctaactgc ctgaacatac tttttatgtc gataatctct ttgtctttac
     4981 gccccttcag caggtcaaga ccaggtacta tctgcctgtc gatttctatc gttatttgat
     5041 tgggcgtgag gaccagtctg tcaatgagca agtgatgatt aagtgcattg accagcaact
     5101 caaggtcaat cgactcttgg tagaccagct tgatttgtcc caagtgagtc atcctaaaat
     5161 gcgagaatat ctgctgaatc atattgaaat cacgacggtg atttccagta ccctgctcaa
     5221 ccgatctgaa acagcggagc atctggcaaa aaaacgccaa ttgtggacct atattcagca
     5281 ggaaaatcca aaagtctttc aggctattcg taagaccatg ttgagccgtt tgaccaaaca
     5341 ttctgtcttg ccagatcgca aactgtccaa tgtcgtctat caaatcacca aatctgttta
     5401 tggatttaat taatataagt gttttataag agggatttaa gaaaaatttt aactttttct
     5461 tagtcctttt taatttcagg agattatact agagtcatca aataaagaaa gactctaagg
     5521 agaatcctat gaaattcaat ccaaatcaaa gatatactcg ttggtctatt cgccgtctca
     5581 gtgtcggtgt tgcctcagtt gttgtggcta gtggcttctt tgtcctagtt ggtcagccaa
     5641 gttctgtacg tgccgatggg ctcaatccga ccccaggtca agtcttacct gaagagacat
     5701 cgggaacgaa agagggtgac ttatcagaaa aaccaggaga caccgttctc actcaagcga
     5761 aacctgaggg cgttactgga aatacgaatt cacttccgac acctacagaa agaactgaag
     5821 tgagcgagga aacaaactct tctagtctgg atacactttt tgaaaaagat gaagaagctc
     5881 aagaaaatcc agagctaaca gatgccttaa aagaaactgt agatacagct gatgtggatg
     5941 ggacacaagc aagtccagca gaaactactc ctgaacaagt aaaaggtgga gtgaaagaaa
     6001 atacaaaaga cagcatcgat gttcctgctg cttatcttga aaaagctgaa gggaaaggtc
     6061 ctttcactgc cggtgtaaac caagtaattc cttatgaact attcgctggt gatggtatgt
     6121 taactcgtct attactaaaa gcttcggata atgctccttg gtctgacaat ggtactgcta
     6181 aaaatcctgc tttacctcct cttgaaggat taacaaaagg gaaatacttc tatgaagtag
     6241 acttaaatgg caatactgtt ggtaaacaag gtcaagcttt aattgatcaa cttcgcgcta
     6301 atggtactca aacttataaa gctactgtta aagtttacgg aaataaagac ggtaaagctg
     6361 acttgactaa tctagttgct actaaaaatg tagacatcaa catcaatgga ttagtt
//
LOCUS       DAVKGU010000094         6267 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKGU010000094 DAVKGU010000000
VERSION     DAVKGU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.095"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            202..1647
                     /gene="cps4A"
                     /locus_tag="VW077_001801"
     CDS             202..1647
                     /gene="cps4A"
                     /locus_tag="VW077_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU9455773.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNAASNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYGTIINSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVLEIDDSSLAVVKAAIQDVMEGR"
     gene            1649..2380
                     /gene="cps4B"
                     /locus_tag="VW077_001802"
     CDS             1649..2380
                     /gene="cps4B"
                     /locus_tag="VW077_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU9455774.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHVIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPQKIVMDQLI"
     gene            2389..3081
                     /gene="cpsC"
                     /locus_tag="VW077_001803"
     CDS             2389..3081
                     /gene="cpsC"
                     /locus_tag="VW077_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU9455775.1"
                     /translation="MKEQNTIEIDVFQLFKTLWKRKLMILIVALVTGAGAFAYSAFIV
                     KPEYTSTTRIYVVNRDQGDKPGLTNQDLQAGSYLVKDYREIILSQDVLEKVATNLKLD
                     MPAKTLASKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFGFLGGAVVTVSAVLLIELLDTRVKRPEDVEDVLQIP
                     LLGVVPNLDKMK"
     gene            3091..3771
                     /locus_tag="VW077_001804"
     CDS             3091..3771
                     /locus_tag="VW077_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU9455776.1"
                     /translation="MPTLEISQAKLDFVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     LGEGKSTTSTNIAWAFAHAGYKTLLIDGDIRNSVMLGVFKARDKITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRKCDASILVTEAGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSVDKYGSY
                     GDYGKNKK"
     gene            3786..5153
                     /locus_tag="VW077_001805"
     CDS             3786..5153
                     /locus_tag="VW077_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9455777.1"
                     /translation="MNGKIVKSSLAIIQSFLVILLTYLLSAVREAEIVSTTAIALYIL
                     HYFVFYISDYGQNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LTVHALLVYVLNLFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNEVVGEL
                     VAVSVLDKPDFQHDCLKVVAEGEIVNFATHEVVDEVFINLPSKKYNIGELVSQFETMG
                     IDVTVNLNAFDRSLARNKQIREMAGLNVVTFSTTFYKTSHVIAKRIIDIVGALVGLIL
                     CGLVSIVLLPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQISGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            5157..5606
                     /locus_tag="VW077_001806"
     CDS             5157..5606
                     /locus_tag="VW077_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEU9455778.1"
                     /translation="MKICLVGSSGGHLTHLYLLKPFWKDKERFWVTFDKADARSVLSE
                     EVVYPCYYPTNRNIKNLIKNTFLAYKILKKERPDLIISSGAAIAVPFFYIGKLFGSKT
                     VYIEVFDRIDAPTITGKLVYPVTDRFIVQWEEMKKIYPKAINLGGIF"
     gene            5606..6085
                     /locus_tag="VW077_001807"
     CDS             5606..6085
                     /locus_tag="VW077_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEU9455779.1"
                     /translation="MIFVTVGTHEQQFDRLIKEFDRLKEENLIQEEVFIQTGYSNYVP
                     KYCKWEKIISYEKMNQFIEEADTIVTHGGPATFMAIIAKGKTPIVVPRQKKFGEHVND
                     HQMNFCSKISSEGYELSLINDISELQYSLKQEKKTKFYSNNANFLKQLSKIIEDLIV"
BASE COUNT         2007 a         1008 c         1348 g         1904 t
ORIGIN      
        1 ttagattgaa actagaatag tacacatctg cttctaaaac attgttagaa atcgatttga
       61 ctgtccttat ttcattttat tatatttttg gttcgcggga agtctactaa gatacttaaa
      121 gatgcagata gtaaaaaaaa tgtagacatt accgtaaaaa agtgatataa tcgtatgatg
      181 ttcaaggtat aggtgttaat catgagtaga cgttttaaaa aatcaggttc acagaaagtg
      241 aagcgaagtg ttaatatcgt tttgctgact atttatttat tgttagtttg ttttttattg
      301 ttcttaatct ttaagtacaa tatccttgct tttagatatc ttaacctagt ggtaactgcg
      361 ttagtcctac tagttgcctt ggtagggcta ctcttgatta tctataaaaa agctgaaaag
      421 tttactattt ttctgttgct gttctctatc cttgtcagct ctgtgtcgct ctttgcagta
      481 cagcagtttg ttggactgac caatcgttta aatgcggctt cgaattactc agaatattcg
      541 atcagtgtcg ctgttttagc agatagtgag atcgaaaatg ttacgcaact gacgagtgtg
      601 acagcaccga ctgggactga taatgaaaat attcaaaaac tactagctga tatcaagtca
      661 agtcagaata ccgatttgac ggtcaaccag agttcgtctt acttggcagc ttacaagagt
      721 ttgattgcag gggagactaa ggccattgtc ctaaatagtg tctttgaaaa tatcatcgag
      781 tcagagtatc cagactacgc atcgaagata aagaagattt ataccaaggg attcactaaa
      841 aaagtagaag ctcctaagac gtctaagaat cagtctttca atatctatgt tagtggaatt
      901 gacacctatg gtcctattag ttcggtgtcg cgatcagatg tcaatatcct gatgactgtc
      961 aatcgagata ccaagaaaat cctcttgacc acaacgccac gtgatgccta tgtaccaatc
     1021 gcagatggtg gaaataatca aaaagataaa ttaacccatg cgggcattta tggagttgat
     1081 tcgtccattc acaccttaga aaatctctat ggagtggata tcaattacta tgtgcgattg
     1141 aacttcactt ctttcttgaa attgattgac ttattgggag gggtagatgt tcataatgat
     1201 caagagtttt cagctctaca tgggaagttc catttcccag tagggaatgt ccatctagac
     1261 tctgagcaag ctctaggttt tgtacgtgaa cgctactcac tagccgatgg agaccgtgac
     1321 cgtggtcgca accaacaaaa ggtgattgtg gctatccttc aaaaattaac gtcaaccgaa
     1381 gcactgaaaa attatggtac gatcattaat agcttgcaag attctatcca aacaaatatg
     1441 ccacttgaga ccatgataaa tttggtcaat gctcagttag aaagtggagg gaattataaa
     1501 gtaaattctc aagatttaaa agggacaggt cggatggatc ttccttctta tgcaatgcca
     1561 gacagtaacc tctatgtgct ggaaatagat gatagtagtt tagctgtagt taaagcagct
     1621 atacaggatg tgatggaggg tagatgaaat gatagacatc cattcgcata tcgtttttga
     1681 tgtagatgac ggtcccaagt caagagagga aagcaaggct ctcttggcag aatcctacag
     1741 gcagggggtg cgaaccattg tctctacctc tcaccgtcgc aagggcatgt ttgaaactcc
     1801 ggaagagaag atagcagaaa actttcttca ggttcgggaa atagctaagg aagtggcgag
     1861 tgacttggtc attgcttacg gggctgaaat ttactacaca ccagatgttc tggataagct
     1921 ggaaaaaaag cggattccga ccctcaatga tagtcgttat gccttgatag agtttagtat
     1981 gaacactcct tatcgcgata ttcatagcgc cttgagcaag atcttgatgt tgggaattac
     2041 tccagtcatt gcccacattg agcgctatga tgctcttgaa aataatgaaa aacgcgttcg
     2101 agaactgatc gatatgggct gttacacgca agtaaatagt tcacatgtcc tcaaacccaa
     2161 actttttggc gaacgttata aattcatgaa aaaaagagct cagtattttt tagagcagga
     2221 tttggttcat gtcattgcaa gtgatatgca caatctagac ggtagacctc ctcatatggc
     2281 agaagcatat gaccttgtta cccaaaaata cggagaagcg aaggctcagg aactttttat
     2341 agacaatcct caaaaaattg taatggatca actaatttag gagaaatgat gaaagaacaa
     2401 aacacgatag aaatcgatgt atttcaatta tttaaaacct tgtggaaacg caagctaatg
     2461 attttaatag tggcacttgt gacaggtgcg ggggcttttg catatagcgc ttttattgtt
     2521 aagccagaat atacgagtac tacgcgaatt tacgtagtga atcgcgatca aggagacaag
     2581 ccggggctga caaatcagga tttgcaggca ggatcttatc tggtaaaaga ctaccgtgag
     2641 attatccttt cgcaggatgt attggaaaaa gtagcgacaa atttgaagtt ggatatgcca
     2701 gcaaaaacgt tagccagcaa agttcaagtg actgtaccag ctgacactcg tatcgtctca
     2761 atctctgtca aagataaaca gccagaagaa gccagtcgta tcgctaattc tctacgagaa
     2821 gttgctgcag aaaagatcgt cgctgtaacg cgagtatctg atgtaacgac acttgaagaa
     2881 gcgcgaccag ctacgactcc ctcttctcca aatgttcgac gcaattcctt gtttggtttt
     2941 cttggaggag cagtcgtaac agtaagtgct gttcttttga ttgagttgct cgacacccgt
     3001 gtgaaacgtc ctgaagatgt cgaagatgta ctgcaaattc cacttttagg ggtcgttcca
     3061 aatttggaca aaatgaaata ggaggaagtt atgccaacat tagaaatctc acaggcaaaa
     3121 ttggattttg taaaaaaggc agaggaatat tataacgctt tgtgcacgaa cctacagtta
     3181 agtggagatg atttgaaagt attttctatc acttctgtga aactaggaga aggaaaatca
     3241 acgacttcca ccaatatcgc ttgggctttt gcgcatgcag gttacaaaac gctgctgatt
     3301 gatggagata ttcgcaattc tgttatgtta ggtgtcttta aagcaaggga taagattaca
     3361 ggcctgacag aatttttatc aggaactaca gatctatcac aagggctttg tgataccaat
     3421 atcgaaaatc tctttgtaat tcaggctggc tctgtgtcac cgaatccgac agctcttctt
     3481 caaagtaaga atttcagtac aatgcttgaa accttgcgta aatattttga ctacatcatt
     3541 gtagatactg ctcctgtcgg tgtcgtgatt gatgcggcta ttattacgcg aaaatgcgat
     3601 gcttctattt tagtgacgga ggcaggtgaa ataaatcgac gggatattca aaaagcaaaa
     3661 gaacagttgg aacacacagg gaagccgttt ttgggagttg tgttgaataa attcgatact
     3721 tcagtagaca aatacggttc ttatggagat tacgggaaaa ataaaaaata ggtcggagga
     3781 cagagatgaa tggaaaaata gtaaagtctt cattggccat aatccagagt tttcttgtta
     3841 ttttattgac ttatctcctt agtgctgtga gagaagcgga gattgtttca acaacagcta
     3901 ttgcacttta tatcctccat tattttgtct tttatattag tgattatgga cagaatttct
     3961 ttaaaagggg atatttgatt gaacttgtcc agacattgaa atatatccta ttctttgcac
     4021 tagcgattag tatttctaat tttttcttag aggatcgatt tagtatttcc agacgaggca
     4081 tgatttactt cctcacagta catgctctct tagtctatgt gctaaaccta tttatcaagt
     4141 ggtattggaa gcgggcttat cccaacttta aaggaagtaa gaagattctc ctacttacag
     4201 caacttctcg tgtcgaaaag gtactggata gattaataga atcaaatgag gttgttgggg
     4261 agttggtagc tgtcagtgtc ttagataaac cagattttca gcatgattgt ttaaaggtag
     4321 tagcagaggg ggagatagta aactttgcga ctcatgaggt ggtcgatgaa gtctttatca
     4381 atcttccaag taaaaaatac aatattggag agcttgtctc tcagtttgaa acgatgggaa
     4441 ttgatgtaac agttaatcta aatgcttttg atcgtagttt ggcacgtaac aagcaaattc
     4501 gtgagatggc aggattaaac gttgtgactt tttctacaac attttataag actagtcatg
     4561 taattgctaa gcggattatt gatatcgtgg gtgcattggt agggctgata ctatgtggtt
     4621 tagtcagtat tgtattgctt cctttgattc gaaaggatgg gggctctgct atttttgctc
     4681 agacgcgtat aggaaaaaat ggtcgtcagt tcacttttta taagtttcgc tctatgtgtg
     4741 tagatgccga ggcgaaaaaa agagaactca tggaacaaaa taccatgcag ggtggaatgt
     4801 ttaaggtgga cgatgatcct cgtatcacga aaattggtcg ttttatacgg aagactagct
     4861 tggacgagct accacagttt tataatgttc taaagggaga tatgagtttg gttggcacac
     4921 ggccaccgac agtggacgag tatgaacact ataccccaga acaaaaacgt cggctaagtt
     4981 ttaaacctgg tataacaggc ttatggcaga tcagtggacg aagtgagatt aagaattttg
     5041 atgaagttgt caaattagat gtagcctata tagacgattg gacaatttgg aaagacattg
     5101 aaattttatt gaagacagtt aaagttgtat ttatgagaga cggagcgaag taggaaatga
     5161 aaatatgttt agttggatct agtggaggtc atctgactca tttatatctt ttaaaacctt
     5221 tctggaaaga taaagaaaga ttttgggtta cttttgataa ggcggacgct cggagtgtac
     5281 tctctgagga agttgtatat ccctgttatt atccaaccaa tcgtaatata aaaaatttaa
     5341 taaaaaatac ttttctcgcc tataaaattt taaaaaaaga gagaccagat ttgattattt
     5401 cttctggtgc cgctatcgct gttcctttct tttatattgg gaagttattt ggatcaaaga
     5461 cagtgtatat agaagtgttt gatagaattg atgctccaac aataacggga aaacttgttt
     5521 atccagtaac ggataggttt attgttcaat gggaagaaat gaaaaaaatt tatccgaaag
     5581 caattaatct aggaggaata ttctgatgat ttttgttact gtagggacac atgaacaaca
     5641 atttgatcgt ttgataaaag aatttgatcg tttgaaagaa gagaatctaa ttcaagagga
     5701 agtatttatt caaacaggat attctaatta tgtcccaaaa tattgtaaat gggaaaaaat
     5761 aatatcttat gaaaaaatga atcagtttat tgaggaagct gatacaattg ttacccatgg
     5821 tggtccagca acttttatgg caattattgc taaaggaaaa actccaatag ttgttccgag
     5881 gcaaaagaaa tttggggagc atgtgaatga tcatcagatg aatttttgtt ctaaaatatc
     5941 ctctgaaggg tatgaattat ctctgattaa cgatataagc gaattgcagt atagtttaaa
     6001 gcaagaaaag aaaactaaat tttactctaa taatgcaaat tttcttaagc agttatcaaa
     6061 aataattgaa gatttaatag tataaaatga gattgtttct tcttattgca gatgaattag
     6121 aaataaatca ggttgtgagt aaagcaacag tgccaaagtg tatttgtaat taagaataaa
     6181 ataaacacca tttcaatatc tgaaaataat tctcaaggca tagtttgaat tatggatata
     6241 gattattttg ctaagtcttc taatcga
//
LOCUS       DAVKGU010000095         6179 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKGU010000095 DAVKGU010000000
VERSION     DAVKGU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.096"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(1..378)
                     /locus_tag="VW077_001808"
                     /pseudo
     CDS             complement(1..378)
                     /locus_tag="VW077_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            503..961
                     /locus_tag="VW077_001809"
     CDS             503..961
                     /locus_tag="VW077_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9455780.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            974..1582
                     /locus_tag="VW077_001810"
     CDS             974..1582
                     /locus_tag="VW077_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455781.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1653..1985
                     /locus_tag="VW077_001811"
     CDS             1653..1985
                     /locus_tag="VW077_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455782.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1996..3411
                     /gene="ply"
                     /locus_tag="VW077_001812"
     CDS             1996..3411
                     /gene="ply"
                     /locus_tag="VW077_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9455783.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALMKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            4292..5008
                     /locus_tag="VW077_001813"
     CDS             4292..5008
                     /locus_tag="VW077_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9455784.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            5132..5602
                     /locus_tag="VW077_001814"
     CDS             5132..5602
                     /locus_tag="VW077_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9455785.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            5618..>6179
                     /locus_tag="VW077_001815"
     CDS             5618..>6179
                     /locus_tag="VW077_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455786.1"
                     /translation="MILLAILFTCFSVYLELEVPTYISKITDLLGSQETNLDELWQPA
                     SMMMGMSFLAFLSVVAVGFFASRVAASYTSRLRSDIFNRVLDYSQTEIKKFSIPSLLT
                     RTTNDITQVQMLITMGLQVVTRGSIMAIWAIGKILGHSEYWLWAVLVVVIVNVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESL"
BASE COUNT         1885 a         1068 c         1390 g         1836 t
ORIGIN      
        1 ataataccag caatcaaatt cattcgtaat ccgaagcgtt tacgatgatt tcgataggtt
       61 gttgaaaaca ttttaaacgt ttttactttg acaaagatgt tctcaacctt gcttctctcc
      121 ttagatagcg catggttaca ggctttatct tcaactgtta gcggcttgag tttgctggat
      181 ttacgtggaa tttgtgcttg aggatatatc ttcatgagcc cttgataacc actgtcagcc
      241 aagattttac cagcttgtcc gacttttttg ataagctgtt tttaatacag ctaacatctc
      301 tttaaaagtg gtacgctgaa cactaacaag acgcttaaat cgtgcatcag ttagttgttt
      361 acttgcttca taattcatag aactactata ccatattttg tttcgcagga agtctaataa
      421 gttatagtcc agtttttact atactttgat attcaaatat gttatactat ggttatcaaa
      481 gtaaatgaaa ggagttacga atatgacgcc agaagaaatg tacctgacag agcgattaga
      541 cgtacagata gctcattttt taaagaaaag cgttcaacat cgtaggcgct ataaggtatt
      601 aaaaataaca gaaatcgtgg caggtttcct catagctgtc ttttgtgcta ttcctatgcc
      661 aggtgatcgc taccgtttga tttcggttgc cttatccagt ctcggcttgc tgtgtgaggg
      721 gattatcaat ttgtataatg caaaggaaaa ttggatttct taccaaaaaa ctgcgcaact
      781 cctggagaaa gaaaaattcc tctatcaatg ccaaacggag aaatatgcag gaaagaccaa
      841 ggcttttgcc ctatttgtca agacatgcga aggtcttatc tcagaggaga ttaaccagtg
      901 ggaaagtatc cagtcaaaag aagtggcagc tagtgcagat gctccagtta aaaaagagta
      961 ggaggtagag gaaatgtctc aatccagtta cctgtcgccc ttgctctggt taaaaaaaga
     1021 agccgataag gaaaagatga gcgcgaccca gtgccagata tttttctttt actatcaaat
     1081 gtttgagctc ttatttgcta gagaaagcga catgaaagac ttatgtctgg gaacgaaagg
     1141 tttttatttc tcgcagttag agaaaaattt gctttctgga gtttcccgat ttctaaaaaa
     1201 cttggagggg aaagtaactc tcaaggctaa ccaagaagta tcagctcgca aagccctttt
     1261 tctagccttg acaactagcc aatcagattg gcaggagtta gctcctgttt ttgattttta
     1321 tcagactatc gggaggcttg aaaatccttc tctcttgagt tctcaggaca gacaacatct
     1381 gatgtggatt taccagtcag ctttggagaa ggattatatt gtcaaggtta ttggcgacaa
     1441 gcattttgta ttgaagagac aagatgctac taaattgaca gcgcgccaaa ctcaaacttt
     1501 ggaaattctg agtcaatcag aagacttggt caatcctgtc tatgttacat taggagaaaa
     1561 gggggtgctc ttgcttgatt aagagaggag atgttgtagc tctttatttg ccttttccga
     1621 ctattagtag cgatttggct gtgaagaatc atatgtatat ctgtattgac aacagcatga
     1681 ctaaaaacaa agagttggtt aaaaatcaga ccttcaaacc agctctcttg accagacgtt
     1741 tggtcaagaa ctttatgata gaagagccgg atctagctcg taatcctttt acaagaccaa
     1801 ccttgattga cttagataag gtatttatgt tggataatac ggttattccg acttcttatc
     1861 tagccagacg gcgacgcaat gtctcagaag aattgtacga ggaaattttg gatcacttag
     1921 tccaaccacg gctgatttcg ctgaacaagt ctgagtttat gcaactcaat ccaggaactt
     1981 attaggaggt agaagatggc aaataaagca gtaaatgact ttatactagc tatgaattac
     2041 gataaaaaga aactcttgac ccatcaggga gaaagtattg aaaatcgttt catcaaagag
     2101 ggtaatcagc tacccgatga gtttgttgtt atcgaaagaa agaagcggag cttgtcgaca
     2161 aatacaagtg atatttctgt aacagctacc aacgacagtc gcctctatcc tggagcactt
     2221 ctcgtagtgg atgagacctt gttagagaat aatcccactc ttcttgcggt tgatcgtgct
     2281 ccgatgactt atagtattga tttgcctggt ttggcaagta gcgatagctt tctccaagtg
     2341 gaagacccca gcaattcaag tgttcgcgga gcggtaaacg atttgttggc taagtggcat
     2401 caagattatg gtcaggtcaa taatgtccca gctagaatgc agtatgaaaa aataacggct
     2461 cacagcatgg aacaactcaa ggtcaagttt ggttctgact ttgaaaagac agggaattct
     2521 cttgatattg attttaactc tgtccattca ggtgaaaagc agattcagat tgttaatttt
     2581 aagcagattt attatacagt cagcgtagac gctgttaaaa atccaggaga tgtgtttcaa
     2641 gatactgtaa cggtagagga tttaaaacag agaggaattt ctgcagagcg tcctttggtc
     2701 tatatttcga gtgttgctta tgggcgccaa gtctatctca agttggaaac cacgagtaag
     2761 agtgatgaag tagaggctgc ttttgaagct ttgatgaaag gagtcaaggt agctcctcag
     2821 acagagtgga agcagatttt ggacaataca gaagtgaagg cggttatttt agggggcgac
     2881 ccaagttcgg gtgcccgagt tgtaacaggc aaggtggata tggtagagga cttgattcaa
     2941 gaaggcagtc gctttacagc agatcatcca ggcttgccga tttcctatac aacttctttt
     3001 ttacgtgaca atgtagttgc gacctttcaa aacagtacag actatgttga gactaaggtt
     3061 acagcttaca gaaacggaga tttactgctg gatcatagtg gtgcctatgt tgcccaatat
     3121 tatattactt gggatgaatt atcctatgat catcaaggta aggaagtctt gactcctaag
     3181 gcttgggaca gaaatgggca ggatttgacg gctcacttta ccactagtat tcctttaaaa
     3241 gggaatgttc gtaatctctc tgtcaaaatt agagagtgta ccgggcttgc ctgggaatgg
     3301 tggcgtacgg tttatgaaaa aaccgatttg ccactagtgc gtaagcggac gatttctatt
     3361 tggggaacaa ctctctatcc tcaggtagaa gataaggtag aaaatgacta ggagaagaga
     3421 atgcttgcga caaaaagagg cgatgatctc tctgcggata tctgaaagtt tatctcttgc
     3481 ctagcgattt ccattggagt atgctcgtga tgttaaaatg agtctatcaa acaggatgtg
     3541 taaaaaagta aaaagggtag ccaattcctt gatatacagg gaattgatta tcctttactt
     3601 gttttagatg attggaaatt caagaatgac acgtatcgga aatattttgc tgtttatatc
     3661 taatacccat ttatggaaaa aatgaaatta aatatgaaca aatcaattgc tagcaatggt
     3721 ttagaagtgc cagtgtacta gtctaacttc tccatcttgg gaacacgtaa attatactct
     3781 tcgaaaatct cttcaaacca ggtcagccct atccgcaacc tcaaaacagt gttttgagca
     3841 acctgcggct agctttctgg tttgttcttt gatttttatt gagtattaga ggtttgctta
     3901 gaaactcttc tattgatttc ttagaataca atagcgccta taagaactgt tggatttcaa
     3961 agtatttact tgaactatga tatgtctgga gtagactgtc aacaaaaggt gcagtatgat
     4021 tgtttttgtc gcttatatct acaaacttca aacagtaatt tgagctgact ttgtcaaact
     4081 tggctgaaac tttctatttg cagataatgg tctagtttgt tttttgaatg tggatgagtg
     4141 tctgttgata aagatagaaa ggcaagaccg tcttagcagg taaagtgcta tcctagcttg
     4201 atttgctaat aaaatccatt tcctctagtg aaaatcaaaa aaacttgtgt tataataaga
     4261 aagattaaaa tgtgaaaaaa ggagattcct aatgggacgt aaatgggcca atatcgtagc
     4321 caagaaaacg gctaaagatg gagctaactc taaagtatat gcaaaatttg gtgtagaaat
     4381 ctatgtagca gctaaaaaag gtgatccaga tccagaatca aactcagctt tgaaattcgt
     4441 tatcgaccgt gctaaacaag cccaagtgcc aaaacacatt atcgataaag cgattgataa
     4501 agccaaagga aacacagacg aaacctttac agaaggacgt tacgaaggtt ttgggccaaa
     4561 tggctctatg ctaattgtgg atactttgac ttctaacgtc aaccgtacag cagccaatgt
     4621 ccgtgcagcc tttggtaaaa acggcggaaa catgggcgct tcaggttctg tttcttacct
     4681 ctttgacaac aaaggtgtta ttgtatttgg aggtgaagat gcggacgcag tctttgagca
     4741 attgctcgaa gcggatgtgg atgtggatga cgtagaagca caagaaggta caatcacagt
     4801 ttacacagcg ccaactgacc ttcacaaggc tatcgttgcc ctccgtgagt ctggtattga
     4861 agaattccaa gtgactgaat tggaaatgat tcctcagtca gaagtggaat tgtcaggcga
     4921 agaccttgaa acctttgaaa aactttacag cgttcttgaa gacgacgaag acgtccaaaa
     4981 gatttatacg aacgtagatg ggttttaatt taacctatag aacatgatcc taagtgagaa
     5041 aatcacttgg gattttttta atcaagaaaa tagttctcat agaaatttaa ttattctcca
     5101 gagaactatt tttatttaga agggggagat tatgggtaaa ccgatgttag tctttaagcg
     5161 ttttggtcat caaattcacc tgatggtgca aaaggaagcc aaacgttgcg gcattgaatt
     5221 tatgggtggg ccgcaaggtc aggttgtgcg ttttttggat aatcgcgaga aaaaccaaga
     5281 cttggtcttg attaaagata tcgagcagga actcaatatt accaagtctg ttgctagtaa
     5341 tctggttaag cgtatagtgc aaaatggttt ggtggaattg gaggcgagtc ctgttgataa
     5401 gcgggctaag tttgttcgtc tgacggacaa agcacgttct cagatgcaac aggttaaggc
     5461 tttttttgaa cgcatagaca agcagttgat ggaagacatt gatgaagatg aattactgat
     5521 ttttgagaaa gttctcggtc aactacaggc aaatatcaag ggaataggag gagagaataa
     5581 agaaattagc caaaagaatt agtagaaaag aatggggatg attttactag ccattctctt
     5641 tacttgcttt tcggtctatc tagagttgga agtgccgacc tatatctcga aaattacgga
     5701 tttgctaggt agtcaagaaa ctaatttaga tgagttgtgg cagccggcaa gcatgatgat
     5761 gggaatgtcc tttcttgcct tcttgtccgt agttgcagtt ggattttttg catcccgagt
     5821 ggcggcttct tatactagta ggctgagaag tgatattttt aaccgagttt tggattactc
     5881 gcagacagag attaagaaat tttcaattcc tagcctcttg acgcgtacta ccaatgacat
     5941 tactcaagtt caaatgttga ttactatggg cttgcaagtg gtaacgcgtg gttcaattat
     6001 ggctatctgg gctattggga agattttagg tcattcagaa tactggctct gggccgtact
     6061 tgtggtagtg attgtcaacg tcctgatgac gaccgttttg atgacgctag cctttccaaa
     6121 acagtccttg attcaggggt tgacagataa actgaacagt atcactcgtg agagtttaa
//
LOCUS       DAVKGU010000096         6119 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKGU010000096 DAVKGU010000000
VERSION     DAVKGU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.097"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            154..462
                     /locus_tag="VW077_001816"
                     /pseudo
     CDS             154..462
                     /locus_tag="VW077_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            626..1285
                     /locus_tag="VW077_001817"
     CDS             626..1285
                     /locus_tag="VW077_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9455787.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1310..2377
                     /locus_tag="VW077_001818"
     CDS             1310..2377
                     /locus_tag="VW077_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9455788.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            2481..3491
                     /locus_tag="VW077_001819"
     CDS             2481..3491
                     /locus_tag="VW077_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455789.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFIIPSDVL
                     QF"
     gene            3503..5194
                     /locus_tag="VW077_001820"
     CDS             3503..5194
                     /locus_tag="VW077_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9455790.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5197..5907
                     /locus_tag="VW077_001821"
     CDS             5197..5907
                     /locus_tag="VW077_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9455791.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2050 a          839 c         1169 g         2061 t
ORIGIN      
        1 attatcgctt accaatattt gctaaacttt cagaaaaaaa gttggagtat atgatgctag
       61 gtgactgcgt catgttagta aacgaaatgg aaataactga tcatagagta gataacttat
      121 ttgaaaaagg gaaaaatgag ataaaagatt caagtagtag atttagcaat aggacatata
      181 aaagctttag aaaaagtatc tgaaaaaaca gatgtttata tctataacct aggctcggga
      241 gaaggcacta gtgtattaca acttgtaaat acatttgaaa gtgttaataa gatccctatt
      301 ccttataaaa tagttccaag acgttcagga gacgttgcaa cttattatgc aaatgcagac
      361 aaagcatata aggaattaaa ttggaggaca acaaaatcga ttgaagacat gtgtagagat
      421 acatggaatt ggcaatcaaa aaatcccaat ggctataatt gatgatactg tctatatgag
      481 tgctgtattt tggaaaaatt acttaacctt ttaatgaaag ataaattaaa aaaaatgttt
      541 gacagcgctt gacaaaggaa acggtttcat tgtataatgt aaacacaatg acaccgatgt
      601 caaatataaa aaagaaggtc tttatatgaa aaacttaaca aaaattaagt tcaaagaaaa
      661 tggggaattt aatcatttcc ctgggaatac agttgtagca aatctttata ctaaacaaga
      721 tttgatggaa gttgttgata ttattcaatc acgttataga gaattaccat ttatcgataa
      781 gtttacttta actccaagga attctattca catgactgta attgaattgt tgtgccatga
      841 aaatcgtgaa acggagtttt ggagcagtaa tcttcctcta gatacacctt tacaggaaat
      901 acatgattac tttgctaaac aacttgaaat ttttccattg ttggatgaag aaattcatat
      961 gcgtataact gaaatgggaa aacaaaacat actagttgaa cctgcagatg aagcttctgc
     1021 aaagagatta gaagaaattc gtacttatgt ctcagaaaaa gcaggtgttt gtttccctaa
     1081 tcatgataga tatcaattcc atatttcaat tgggtatctt cggattcctc taaccgaaga
     1141 ggaggaagaa gagtttacta aagtcagagc agaattaact gaaattttat tagagaagat
     1201 tccaactatt actgtaaacc gtattgacta tactgtattt gaagatatga gacaatttgt
     1261 tccatatcac gaaaaattta aataaaataa taaaaaagga gaacttatca tgaaaaaaat
     1321 gaaagtttgg tctactgtac ttgcaacggg agttgctctt actacacttg ctgcttgctc
     1381 tggaggttca aattctacga ctgcttcttc atctgaagaa aaagctgata aaagtcaaga
     1441 attagttatc tattcgaact cagtctcaaa tggtcgtggt gattggttaa ctgctaaagc
     1501 aaaagaagct ggttttaata taaaaatggt tgatatcgct ggcgctcaat tagcagaccg
     1561 tgttattgct gagaagaata atgcagttgc agatatggta tttggaattg gtgctgttga
     1621 ttcaaataaa attagagatc aaaaattact agtacagtac aagcctaaat ggttagataa
     1681 aattgatcaa tctttatcag ataaagataa ttattataat cctgtgattg ttcaaccatt
     1741 agttttaatt ggggcgcctg atgtaaaaga aatgcctaaa gattggactg aattaggtag
     1801 taagtataaa ggtaaatatt caatttatgg tcttcaaggt ggtacaggac gggcaattct
     1861 agcaagtatc ttagttcgtt accttgatga aaaaggagat ttaggcgtat cagaaaaagg
     1921 atgggaagtt gcaaaagaat actttgcaaa tgcctatact cttcaaaaag gtgaaagctc
     1981 agttgttaaa gcgttagata aagaaagccc aattcaatac ggtatgatct ggggttctgg
     2041 agcgttaatt ggacaaaaag aacaaaatgt tgaattcaaa gttatgactc ctgagattgg
     2101 tgtaccattt gtaactgaac aaactatggt tttaagcact agtaaaaaac aagcgttagc
     2161 taaagaattt attgattggt ttggtcaatc agaaattcaa gtagaatata gtaaaaaatt
     2221 tggatctatt cctgccaata aagatgccct aaaagagtta cctgaagata cgaaaaaatt
     2281 tgttgatcaa gtgaaaccac aaaatattga ctgggaagct gttggaaagc atttggatga
     2341 atgggtagaa aaagctgaat tagaatacgt aaaataagaa ataagagtta ttttttatgt
     2401 ccaagtctct ctattatttc taaatttaag gaagaaataa agagaggctt ttggttttag
     2461 taatagaggg agttagtgag atgattaaat ttgataatat tcaaattaaa tatggtgatt
     2521 ttgttgcaat tgataatctg aatttagata tacatgaagg ggaattcttt acatttcttg
     2581 ggccttcagg atgtggtaaa tcaactactt tgagagcatt ggtaggtttt ttagatccat
     2641 catcaggaag tattgaagtt aatggaacag atgtcactca tttggaacct gaaaagcgtg
     2701 gaattggtat tgtatttcaa tcttatgcgc tatttccaac tatgactgtt tttgataata
     2761 ttgcatttgg tttaaaagtt aagaaggtag ctccagatgt tattaaagct aaagtatcag
     2821 cagtggcagc aaaaattaag atctctgatc aacagttaca gcgtaatgta tcagaattat
     2881 ctgggggtca acaacaaagg gtagcattgg ctcgtgctct ggttcttgaa cctaaaattc
     2941 tttgtctaga tgaaccattg tcaaaccttg acgcaaaatt acgtgtagat ttgagaaaag
     3001 agttgaaaag acttcaaaaa gagttaggta ttactacttt atatgttact catgatcaag
     3061 aggaagcctt gactttatct gatagaattg cagtctttaa caatggatac atcgaacagg
     3121 tcggtacacc agtagagatt tatcataatt ctcaaactga atttgtatgt gattttattg
     3181 gagatattaa tgttttgacc gatgaaacag tccacgaagt attattgaaa aatacaagcg
     3241 ttttcttaga ggataaaaaa ggatacattc gattagagaa agttcgattc aatcgtgaaa
     3301 ctgaacaaga ttttattcta aaagggacaa ttattgatgt tgagttttct ggagttacaa
     3361 ttcactatac aataaaagtt tctgaaagtc agattcttaa tgtaacaagt attgatagtc
     3421 aggctgctat tagatctgtc ggagaaagtg tggaattatt tatcatacca tcagacgttc
     3481 tgcaatttta aggaggtgca gtatgcgtca taaattaaat ttaaaagatt ggcttattcg
     3541 tttagggtta atctggttct tagtaacatt tattatttat ccaaactttg atctagtagt
     3601 gaatgtattt gtaaaaggag gagaattttc ccttgatgct gtacatcgtg ttctaaaatc
     3661 tcagagggca cttcagagta ttatgaacag ttttaagtta gcattttcac tcattattac
     3721 agttaatgtc gtaggtattc tttgtgttct atttacagag tactttgata ttaaaggtgc
     3781 taaaatttta aaattaggtt atatgacctc tttaatttat ggaggagtgg ttttagcgac
     3841 tggatataaa tttgtctatg gtccttatgg attgattaca aaatttttac aaaatgttat
     3901 cccttcttta gactctaact ggtttattgg gtatggtgca gtcttattca ttatgacatt
     3961 ttcaggaact gctaatcata cattgttttt aacaaataca attcgaagcg ttgactatca
     4021 cactattgag gctgctcgaa atatgggagc aaaaccattt actgttttcc gaaaagtagt
     4081 gttaccaacc ttaattccaa ctctatttgc acttactatt atggtttttc ttagtggttt
     4141 atctgcagta gcagcaccca tgattgttgg tggtaaagaa tttcaaacta taaatccaat
     4201 gattattaca tttgcaggga tggggaattc tcgtgattta gctgccttac ttgcaattat
     4261 tttaggtatt gcaactacaa ttttgcttac tatcatgaat aagatagaaa aaggtggaaa
     4321 ttatatttct atctctaaga ctaaagcgcc tcttaaaaaa caaaaaattg cgtctaagcc
     4381 ttggaatatc attgctcaca ttgtagcata tggattgttc acagttttca tgcttccact
     4441 aatttttata gtattatact catttacaga tccagttgca attcaaacag gtaacttaac
     4501 attatcaaac tttactttag aaaattatcg cttattcttt agtaatagtg cggcattctc
     4561 tccattcttg gtcagcttta tttattctat tattgctgcg acaacagcaa caattctcgc
     4621 agttgtattt gctcgtgttg tcagaaaaca taaatctcgt tttgatttct tatttgaata
     4681 tggtgctcta cttccttggt tactaccaag tacactttta gcagtaagtt tattatttac
     4741 ttttaatcag ccacaatttc ttgttttgaa tcagattttg gtaggtagtt tggtaattct
     4801 acttattgca tatatagttg taaaaatccc attttcttat agaatggtac gtgctatttt
     4861 atttagtgtt gatgatgaga tggaagatgc agcaagaagt atgggtgctt caccttttta
     4921 tactatgatg aaggttatca ttccatttat tttaccggtt gttctctctg ttattgcttt
     4981 aaactttaac tctttattaa ctgacttcga cttatctgta ttcctttacc atcccctagc
     5041 tcaaccatta ggtattacga ttcgatctgc aggtgatgaa acagcaacat ctaatgcaca
     5101 agctctggta tttgtttata caattgttct gatgattatt tctggaacgg tattatactt
     5161 cacacaaaga ccggggcgta aagtaaggaa ataatcatga cagccactag tcttgggtta
     5221 tcaaatattg aaatagttgt caggattgtt ttatcagtag tcattggtag tataattggt
     5281 ttagagagag ggagcaaatc ccagcctgca ggcatccgaa cttatagtat tgtttgtcta
     5341 gctgcatgtt tgattatgat gacgaatgaa tacgtatctt ataaatttgg gacaggagat
     5401 cctacacgat taggagctca agttatatca ggtgtgggtt ttctaggcgc tggaacgatt
     5461 cttattacag ataaaaagaa aattacaggt ctgacaactg cagcaggcat ttgggcttcg
     5521 gcaggaattg gattagctat tggagtaggt ttttatgagg gagctctttt agtagccatt
     5581 tctgtttggg gtgtgatatc catgttccaa ccactaaaaa aatatctgca aaatcgttct
     5641 aaaatgattg aattgtatat agtggttaaa tctacagaag cctataatcg tgtattggtt
     5701 tattgtgcag aaaatggtat cagaatgacc gattcaagaa cagcgtttgg agatgttaat
     5761 tctgacagaa ttgaatactt tgatgttccg gacaaaaaaa tagcgtcatt tattactctg
     5821 gaattatcag gtagatttga acatcttcgg ctgatggaag aaattgctaa tattgtaggt
     5881 gttatttatg tcgaagaaat tagctgaaat ttagtaaaca aagggacaga tgtttttgtc
     5941 cttttgcttt ttagatgttt taatcatcta atttttgagt agatttttgc tcagaggttt
     6001 ttatgaaaga ttataaaatc aattttgatt taggtaaaat agaatatttt gataataatt
     6061 gtttgataca agtatacaaa tttatttctt tttatgatat ttgtgaaatg gtgtttgcc
//
LOCUS       DAVKGU010000097         6007 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKGU010000097 DAVKGU010000000
VERSION     DAVKGU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.098"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            69..311
                     /locus_tag="VW077_001822"
     CDS             69..311
                     /locus_tag="VW077_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9455792.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            313..567
                     /locus_tag="VW077_001823"
     CDS             313..567
                     /locus_tag="VW077_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9455793.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            614..1309
                     /locus_tag="VW077_001824"
     CDS             614..1309
                     /locus_tag="VW077_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU9455794.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMTDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
     gene            complement(1646..2632)
                     /locus_tag="VW077_001825"
     CDS             complement(1646..2632)
                     /locus_tag="VW077_001825"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9455795.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            2826..5294
                     /gene="gyrA"
                     /locus_tag="VW077_001826"
     CDS             2826..5294
                     /gene="gyrA"
                     /locus_tag="VW077_001826"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9455796.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            5294..>6007
                     /gene="srtA"
                     /locus_tag="VW077_001827"
     CDS             5294..>6007
                     /gene="srtA"
                     /locus_tag="VW077_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9455797.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWNAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTA"
BASE COUNT         1892 a          996 c         1311 g         1808 t
ORIGIN      
        1 tttttatatt gactgaacac ctaaaaagta atacaatggt gttaccatta aaaaagggag
       61 cacaaaagat gactactatt acattaaaag tttctgaagc tgataaaaca tttatgaaag
      121 caatggctaa gtttgaagga gtttcccttt cggaacttat tcgcaccaaa actcttgaag
      181 ccctagaaga tgaatacgat gctcgtgtgg cagatttagc ctatcaagag tatttagaag
      241 acttggaaaa aggagttgaa cccattactt gggaagaaat gatgcatgat ttaggcttga
      301 aggatgaata atttgtataa attagttcca acaagacgtt ttatcaagca attgaaaaaa
      361 ttggaccgtt atacgcagaa gctaattaca aactatttac aaaccaatgt tttggaagac
      421 ccaagacgac acggaaaggc tttggttggt aatcgcgttg gtcaatggcg ctatagaatt
      481 ggtaattatc gagttatcgt acaaattgta gatgatgaat tagtcgttgc tactctagaa
      541 gttggtcatc ggagagatat ttattgaatt aaagctgagt aataacagta tagaaagtaa
      601 ggagagtaaa aatttgaaga aaaaatggtt ttttgcagat tattatgaca caacaattat
      661 tttacttgct ttaatctctg ttattctggt attacttgga tttgctgaga tgactgattt
      721 ggacaatccg ccttatagta ttattgattt agtaatctgg ggtgtttttg taattgatta
      781 tagttggcgc ttttttatta ctaaaagaaa atggcgcttt attcttgaaa atatttttga
      841 tttattgacc attctccctt taaatgctat ttttacagtg tttcgattag gacgtatctt
      901 ccgcttagca aggctgacaa aattactgaa actgactcgt ttacttagaa taattggact
      961 tacaggtaaa ctagaaagaa aaattagtag attcttacga acaaatggat tgatttatat
     1021 cttgtatgtt aatatcttta ttgttctagt agggagttcg attttatccg ttgttgaaga
     1081 aaaatccttc tcagatagtc tttggtgggc tcttgtgaca gtaactactg ttggttatgg
     1141 tgatattgtt ccagtttccc tttttgggaa atggctagca gttttactga tgcttgtcgg
     1201 tattggaaca atagggatgc taacaagtgc tttgacgaac ttttttgtaa aggataatcc
     1261 agatgaacag ataaaacttg ataaactcaa agacgaatta tcgagctaaa gaatattaat
     1321 agagaaacaa tctgaaaaaa tcgaagaact acattgaatg ataaaagact tagttgaaaa
     1381 aatatcataa aggtgagaca aaataaaact tgattcgagg agaaatcaag taagtcttga
     1441 cacaatagaa cgcatattat caggtataaa aacttgatac tatgcgtttt atagagaaat
     1501 aaaataaaat ctgaacaaat caattaggaa tttaaagcat tttataacaa cgttttagag
     1561 tgatggggtg ctatttcaac ttcaacctac tataatacag aaaaaaacaa ctccctgatg
     1621 attcaaggag ttgtctatag ttaaattagt ttttagaagc ttcttggaat tctggatttt
     1681 tccatgcttc gtcaataata gcttgcaatt ctttagcaga tgcttgcatt ttttgagttt
     1741 ctgcatcgtt caatggaatg tttactggac gaacgatacc atgtgcacca acaacagctg
     1801 gttgaccgat aaagacattc tcaactccgt attgaccttc ttggaatact gaaagtggaa
     1861 gtactgcgtt ttcatcgtca aggattgctt tagtgatacg agcaagggct actgcgatac
     1921 cgtagtatgt tgcacctttt ttgttgatga ttgtgtaggc tgcatcacga acaccttcga
     1981 acaattcaat caattcagct tcttgaacat tttgagtgtc tttaaggaat tcttcaaggt
     2041 ttacaccagc gatgttagcg tgtgaccaaa cagcgaactc agagtcaccg tgttcaccca
     2101 tgatgtaggc gtgcactgaa cgagcatcca catccaattt ttcagcaagt gcttgacgga
     2161 aacgagctga gtcaagtgaa gtacctgaac cgataacgcg ttctttaggg aaaccagaga
     2221 atttccaagt tgagtaagtc aaaacgtcaa ctgggttagc agcaacaagg aagatacctt
     2281 tgaaaccaga ttcaacaact tgagttacga ttgatttgtt gatagcaagg tttttaccta
     2341 caaggtcaag acgagtttca cctggttttt gaggtgcacc tgcagtgatc acaacaaggt
     2401 cagcgtctgc acagtcagag tattgagctg catagatttt tttaggtgaa gtgaaggcaa
     2461 gggcgtgact aaggtcaagc gcatcaccaa cagctttttc atgcaattgt ggaatttcga
     2521 taattccaag ctcttgtgca attccttggt taacaagtgc aaaagcgtaa gatgaaccta
     2581 cagcaccatc accgacaagg ataacttttt tgtgttgttt agttgaagtc attgttttaa
     2641 acatctcctt aattttatta ggggattttc cctagacaac ttcattctat cacttttaaa
     2701 aaactttgtc acgaatatgc cttatagttc tcgatgtaaa cgttttagtg gtttagaggc
     2761 tgaaatagat gggaatttat ggtataatgt tgttacttac taattgtgaa atgaggcatt
     2821 tattaatgca ggataaaaat ttagtgaatg tcaatctgac aaaggagatg aaggcaagtt
     2881 ttatcgacta cgccatgagt gttatcgtag cgcgagctct tcctgatgtt cgagatggct
     2941 taaaacctgt tcaccgtcgc attctctacg gaatgaatga attgggtgtg accccagaca
     3001 aaccccataa aaaatctgct cgtattacag gggatgtcat gggtaaatat cacccacacg
     3061 gggattcctc tatttatgaa gccatggtcc gtatggctca atggtggagc taccgttaca
     3121 tgcttgtaga tggtcatggg aattttggtt ccatggatgg agatagtgct gccgctcaac
     3181 gttataccga ggcacgtatg agcaagattg ctctggaaat gcttcgtgat atcaacaaaa
     3241 atacagttga tttcgttgat aactatgatg ccaatgaacg ggaacccttg gtcttgccag
     3301 cgcgttttcc aaaccttttg gttaatggag caactggtat cgcggttggg atggcaacca
     3361 atattccacc tcataatctg ggtgaaacca ttgatgcagt gaagttggtc atggataatc
     3421 ctgaagtgac taccaaggac ttgatggaag tcttgcctgg accagatttt ccaactggtg
     3481 ctcttgtcat ggggaaatca ggtatccata aggcttatga aacaggtaaa ggttcgattg
     3541 tcctacgttc tcgtacagag attgaaacga ctaagactgg tcgtgagcgc atcgttgtaa
     3601 cagaatttcc ttacatggtc aataaaacca aggtgcatga gcatattgtt cgcttggttc
     3661 aggaaaaacg cattgagggt atcacagcag tacgtgatga gtcaaaccgt gaaggtgttc
     3721 gatttgttat tgaagtcaag cgcgacgcct cagccaatgt tattctcaat aacctcttca
     3781 aaatgaccca aatgcaaacc aattttggtt tcaatatgct cgctatccaa aatggtatac
     3841 cgaaaatttt gtctcttcgt cagattttgg atgcttatat cgagcaccaa aaagaagtgg
     3901 ttgttcgtcg tacacgtttt gataaggaaa aagcggaagc gcgcgctcat atcttagaag
     3961 gtctcttgat tgcgctagac catatcgacg aagtgattcg tatcatccgt gctagtgaaa
     4021 cggatgcgga agctcaagct gagttgatga gcaagtttaa gctttctgaa cgtcaaagtc
     4081 aagctatcct tgatatgcgt cttcgtcgtt tgacaggttt ggaacgcgat aagattcaat
     4141 ctgagtatga tgacctcttg gctctgattg cggatttagc agatattctt gctaagcctg
     4201 aacgtgtttc tcaaattatc aaagacgaat tggatgaagt taaacgtaaa ttttctgata
     4261 aacgccgtac agagttgatg gttggacaga tcttgagtct cgaggatgag gacttgattg
     4321 aagaatcgga tgtcttgatt accctttcta acagaggcta cattaagcgt ttggatcagg
     4381 acgagttcac tgctcaaaaa cgtgggggtc gtggtgtcca aggaacggga gtgaaagatg
     4441 atgactttgt tcgtgagtta gtgtcaacta gcacacatga tcatctgctc ttcttcacaa
     4501 acaagggacg tgtctatcgt cttaaaggtt atgaaattcc tgagtatggt cggactgcca
     4561 aagggctacc agtagtcaat ctcttgaaat tggatgaaga cgaaagtatt cagacggtta
     4621 tcaatgttga gtctgatcgc agtgatgatg cttatctctt ctttacaacc cgtcacggta
     4681 ttgtgaagag aactagtgtt aaggagtttg ccaatattcg tcaaaatggt ctcaaagcgc
     4741 tgaatttaaa ggatgaagat gagttaatca atgtcttgtt ggcagaagga gatatggata
     4801 ttatcattgg taccaagttt ggttatgcag ttcgctttaa tcaatcagcc gttcgtggta
     4861 tgagccgtat cgccactggt gtgaaaggtg ttaaccttcg tgaaggagac acagttgttg
     4921 gtgccagctt gattactgat caagatgagg ttcttattat cacagaaaaa ggatatggta
     4981 agcgtacagt cgctactgaa tacccaacaa aaggtcgtgg tggtaaggga atgcagacag
     5041 ctaaaattac cgaaaaaaat ggcttgctgg ccggtcttat gactgttcaa ggggatgagg
     5101 atttgatgat tatcactgat acaggtgtca tgattcgaac caatcttgcc aatatttcac
     5161 aaacaggacg tgcaactatg ggagttaaag taatgcgcct ggatcaagat gctcagatag
     5221 tgactttcac aacggttgcg gtggcagaaa aagaagaagt tgggacagaa aacgaaacag
     5281 aaggtgaagc ataatgtctc aaaaaaataa taaaaagaaa aacaagcgaa aaaatctgct
     5341 gacaaatatc ctagcaggat ttctgatatt actgtcactg gctttgattt ttaatactca
     5401 aattcgaaat attttcatag tctggaatac caataagtat caagttagcc aggtatcaaa
     5461 agaaaaatta gaagaaaatc aggatacaga aggcaatttt gactttgatt ctgtcaaagc
     5521 tatctcttcg gaagctgttc taacttctca atggaatgct caaaaattac cagttattgg
     5581 gggaattgca attcctgaat tggaaatgaa tttgccgatt tttaaaggac ttgataatgt
     5641 taatctcttc tacggagctg gtacaatgaa acgcgagcaa gtaatgggag aaggaaatta
     5701 tagtctagct agtcaccata tctttggtgt tgataatgct aataaaatgt tattttctcc
     5761 tttagataat gctaaaaatg gcatgaagat ttatctaacc gataaaaata aagtttatac
     5821 ttatgaaata cgtgaagtca aacgtgtgac accggatcgt gttgatgaag ttgatgatag
     5881 agatggggtc aatgaaatca cattagtaac ctgtgaagac cttgctgcta cagaacgtat
     5941 tattgtcaaa ggtgatttga aagaaacaaa agattattca caaacatctg atgaaatcct
     6001 aacagct
//
LOCUS       DAVKGU010000098         5844 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKGU010000098 DAVKGU010000000
VERSION     DAVKGU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.099"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            81..560
                     /locus_tag="VW077_001828"
     CDS             81..560
                     /locus_tag="VW077_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9455798.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            654..725
                     /locus_tag="VW077_001829"
     tRNA            654..725
                     /locus_tag="VW077_001829"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:687..689,aa:Glu,seq:ttc)
     gene            977..2522
                     /locus_tag="VW077_001830"
     rRNA            977..2522
                     /locus_tag="VW077_001830"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2574..2646
                     /locus_tag="VW077_001831"
     tRNA            2574..2646
                     /locus_tag="VW077_001831"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2607..2609,aa:Ala,seq:tgc)
     gene            2771..5672
                     /locus_tag="VW077_001832"
     rRNA            2771..5672
                     /locus_tag="VW077_001832"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5749..>5844
                     /gene="rrf"
                     /locus_tag="VW077_001833"
     rRNA            5749..>5844
                     /gene="rrf"
                     /locus_tag="VW077_001833"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1720 a         1133 c         1672 g         1319 t
ORIGIN      
        1 gagctaaagg gaacagaatg gagaaaatgg aacaaatgtg ttttctaatc tgttagactg
       61 tatctagaaa ggggaaaatt atgattaaag aattgtatga agaagtccaa gggactgtgt
      121 ataagtgtag aaatgaatat taccttcatt tatgggaatt gtcggattgg gagcaagaag
      181 gcatgctctg cttacatgaa ttgattagta gagaagaagg actggtagac gatattccac
      241 gtttaaggaa atatttcaag accaagtttc gaaatcgaat tttagactat atccgtaaac
      301 aggaaagtca gaagcgtaga tacgataaag aaccctatga agaagtgggt gagatcagtc
      361 atcgtataag tgaggggggt ctctggctag atgattatta tctctttcat gaaacactaa
      421 gagattatag aaacaaacaa agtaaagaga aacaagaaga actagaacgc gtcttaagca
      481 atgaacgatt tcgagggcgt caaagagtat taagagactt acgcattgtg tttaaggagt
      541 ttactatccg tacccactag taagtcatgc aaaaaaaatg aaaaaaatta gaaaaagtag
      601 ttgacaaagt ttgaaaagac tgtataatag taagagttga aaataacaac tcaggtccgt
      661 tggtcaaggg gttaagacac cgccttttca cggcggtaac acgggttcga atcccgtacg
      721 gactatggta tgttgcgtca ggagcacttg atgaaaaaaa gtttaaaaaa acttaaaaat
      781 cttcaaaaaa gtgttgacaa gcgaaagcag ttgtgatata ctaatatagt tgtcgcttga
      841 gagaagcaag tgacaaagac ctttgaaaac tgaacaagac gaaccaatgt gcagggcgct
      901 acaacataag ttgtagtact gaacaatgaa aaaacaataa atctgtcagt gacagaaatg
      961 agtgagaact caaactttta atgagagttt gatcctggct caggacgaac gctggcggcg
     1021 tgcctaatac atgcaagtag aacgctgaag gaggagcttg cttctctgga tgagttgcga
     1081 acgggtgagt aacgcgtagg taacctgcct ggtagcgggg gataactatt ggaaacgata
     1141 gctaataccg cataagagtg gatgttgcat gacatttgct taaaaggtgc acttgcatca
     1201 ctaccagatg gacctgcgtt gtattagcta gttggtgggg taacggctca ccaaggcgac
     1261 gatacatagc cgacctgaga gggtgatcgg ccacactggg actgagacac ggcccagact
     1321 cctacgggag gcagcagtag ggaatcttcg gcaatggacg gaagtctgac cgagcaacgc
     1381 cgcgtgagtg aagaaggttt tcggatcgta aagctctgtt gtaagagaag aatgagtgtg
     1441 agagtggaaa gttcacactg tgacggtatc ttaccagaaa gggacggcta actacgtgcc
     1501 agcagccgcg gtaatacgta ggtcccgagc gttgtccgga tttattgggc gtaaagcgag
     1561 cgcaggcggt tagataagtc tgaagttaaa ggctgtggct taaccatagt aggctttgga
     1621 aactgtttaa cttgagtgca agaggggaga gtggaattcc atgtgtagcg gtgaaatgcg
     1681 tagatatatg gaggaacacc ggtggcgaaa gcggctctct ggcttgtaac tgacgctgag
     1741 gctcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc tgtaaacgat
     1801 gagtgctagg tgttagaccc tttccggggt ttagtgccgt agctaacgca ttaagcactc
     1861 cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg cccgcacaag
     1921 cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc
     1981 ctctgaccgc tctagagata gagctttcct tcgggacaga ggtgacaggt ggtgcatggt
     2041 tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccctatt
     2101 gttagttgcc atcatttagt tgggcactct agcgagactg ccggtaataa accggaggaa
     2161 ggtggggatg acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat
     2221 ggctggtaca acgagtcgca agccggtgac ggcaagctaa tctcttaaag ccagtctcag
     2281 ttcggattgt aggctgcaac tcgcctacat gaagtcggaa tcgctagtaa tcgcggatca
     2341 gcacgccgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt
     2401 ttgtaacacc cgaagtcggt gaggtaaccg taaggagcca gccgcctaag gtgggataga
     2461 tgattggggt gaagtcgtaa caaggtagcc gtatcggaag gtgcggctgg atcacctcct
     2521 ttctaaggat aaggaactgc gcattggtct tgtttagtct tgagaggtct tgtggggcct
     2581 tagctcagct gggagagcgc ctgctttgca cgcaggaggt cagcggttcg atcccgctag
     2641 gctccattgg tgagagatca ccaagtaatg cacattgaaa attgaatatc tatatcaaat
     2701 agtaacaaga aaataaaccg aaaacgctgt agtattaata agagtttatg actgaaaggt
     2761 cagaaaataa ggttaagtta ataagggcgc acggtggatg ccttggcact aggagccgac
     2821 gaaggacgtg acaaacgacg atatgccttg ggtagctgta agtaagcgat gatccaggga
     2881 tttccgaatg ggggaaccca acaggtagta cctgttaccc acatctgtta aggatgtgag
     2941 gaggaagacg cagtgaactg aaacatctaa gtagctgcag gaagagaaag caaaagcgat
     3001 tgccttagta gcggcgagcg aaacggcagg agggcaaacc gaagagttta ctcttcgggg
     3061 ttgtaggact gcaatgtgga ctcaaagatt atagaagaat gatttgggaa gatcagccaa
     3121 agagagtaat agcctcgtat ttaaaatagt ctttgtactt agcagtatcc tgagtacggc
     3181 gggacacgtg aaatcccgtc ggaatctggg aggaccatct cccaacccta aatactccct
     3241 agtgaccgat agtgaaccag taccgtgagg gaaaggtgaa aagcaccccg ggaggggagt
     3301 gaaatagaac ctgaaaccgt gtgcctacaa caagttcgag cccgttaatg ggtgagagcg
     3361 tgccttttgt agaatgaacc ggcgagttac gttatgatgc gaggttaagt tgaagagacg
     3421 gagccgtagg gaaaccgagt ctgaataggg cgccttagta tcatgacgta gacccgaaac
     3481 catgtgacct acccatgagc aggttgaagg tgcggtaaga cgcactggag gaccgaacca
     3541 gggcacgttg aaaagtgctt ggatgacttg tgggtagcgg agaaattcca aacgaacttg
     3601 gagatagctg gttctctccg aaatagcttt agggctagcg tcgacattag agattcttgg
     3661 aggtagagca ctgtttgggt gaggggtcca tcccggatta ccaatctcag ataaactccg
     3721 aatgccaatg aattatggtc ggcagtcaga ctgcgagtgc taagatccgt agtcgaaagg
     3781 gaaacagccc agaccaccag ctaaggtccc aaaataattg ttaagtggaa aaggatgtgg
     3841 ggttgcacag acaactagga tgttagctta gaagcagcta ttcattcaaa gagtgcgtaa
     3901 tagctcacta gtcgagtgac cctgcgccga aaatgtaccg gggctaaaac aatttaccga
     3961 agctgtggat acctttatag gtatggtagg agagcgttct atgtgtgatg aaggtatacc
     4021 gtgaggagtg ctggaacgca tagaagtgag aatgccggta tgagtagcga aagacaggtg
     4081 agaatcctgt ccaccgtaag actaaggttt ccaggggaag gctcgtccgc cctgggttag
     4141 tcgggaccta aggagagacc gaaaggtgta tccgatggac aacaggttga tattcctgta
     4201 ctagagtatg tagtgatgga gggacgcagt aggctaacta aagcagacga ttggaagagt
     4261 ctgtctaagc agtgaggtgt gaattgagtc aaatgcttaa ttctataaca ttgagctgtg
     4321 atggggagcg aagtttagta gcgaagttag tgacgtcaca ctgccaagaa aagcttctag
     4381 cgtttaaaca tactctaccc gtaccgcaaa ccgacacagg tagtcgaggc gagtagcctc
     4441 aggtgagcga gagaactctc gttaaggaac tcggcaaaat gaccccgtaa cttcgggaga
     4501 aggggtgctg actttaagtc agccgcagtg aataggccca agcaactgtt tatcaaaaac
     4561 acagctctct gctaaatcgt aagatgatgt atagggggtg acgcctgccc ggtgctggaa
     4621 ggttaagagg agtgcttagc gtaagcgaag gtatgaattg aagccccagt aaacggcggc
     4681 cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg
     4741 aaaggcgtaa tgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt
     4801 gaagatgcag gttacccgcg acaggacgga aagaccccat ggagctttac tgcagtttga
     4861 tattgagtgt ctgtaccaca tgtacaggat aggtaggagt ctaagagatc gggacgccag
     4921 tttcgaagga gacgctgttg ggatactacc cttgtgttat ggccactcta acccagatag
     4981 gtgatcccta tcggagacag tgtctgacgg gcagtttgac tggggcggtc gcctcctaaa
     5041 aggtaacgga ggcgcccaaa ggttccctca gaatggttgg aaatcattcg cagagtgtaa
     5101 aggtataagg gagcttgact gcgagagcta caactcgagc agggacgaaa gtcgggctta
     5161 gtgatccggt ggttccgtat ggaagggcca tcgctcaacg gataaaagct accctgggga
     5221 taacaggctt atctccccca agagttcaca tcgacgggga ggtttggcac ctcgatgtcg
     5281 gctcgtcgca tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc
     5341 ggcacgcgag ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt
     5401 aggaaatttg agaggatctg ctcctagtac gagaggacca gagtggactt accgctggtg
     5461 taccagttgt cttgccaaag gcatcgctgg gtagctatgt agggaaggga taaacgctga
     5521 aagcatctaa gtgtgaaacc cacctcaaga tgagatttcc catgattata tatcagtaag
     5581 agccctgaga gatgatcagg tagataggtt agaagtggaa gtgtggcgac acatgtagcg
     5641 gactaatact aatagctcga ggacttatcc aaagtaactg agaatatgaa agcgaacggt
     5701 tttcttgatt tgaatagata ttcaattttg agtaggtatt actcagagtt aagtgacgat
     5761 agcctaggag atacacctgt acccatgccg aacacagaag ttaagcccta gaacgccgga
     5821 agtagttggg ggttgccccc tgtg
//
LOCUS       DAVKGU010000099         5830 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKGU010000099 DAVKGU010000000
VERSION     DAVKGU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.100"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            2..268
                     /locus_tag="VW077_001834"
     CDS             2..268
                     /locus_tag="VW077_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9455799.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            261..629
                     /locus_tag="VW077_001835"
     CDS             261..629
                     /locus_tag="VW077_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9455800.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            749..2017
                     /locus_tag="VW077_001836"
     CDS             749..2017
                     /locus_tag="VW077_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9455801.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            2014..3291
                     /gene="tilS"
                     /locus_tag="VW077_001837"
     CDS             2014..3291
                     /gene="tilS"
                     /locus_tag="VW077_001837"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9455802.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            3295..3837
                     /gene="hpt"
                     /locus_tag="VW077_001838"
     CDS             3295..3837
                     /gene="hpt"
                     /locus_tag="VW077_001838"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9455803.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            3853..5811
                     /gene="ftsH"
                     /locus_tag="VW077_001839"
     CDS             3853..5811
                     /gene="ftsH"
                     /locus_tag="VW077_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9455804.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMSGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT         2028 a          873 c         1199 g         1730 t
ORIGIN      
        1 gatgagatta gataaatatt taaaagtatc gcgaattatc aagcgtcgta cagtcgcaaa
       61 ggaagtagca gataaaggta gaatcaaggt taatggaatc ttggccaaaa gttcaacgga
      121 cttgaaagtt aatgaccaag ttgaaattcg ctttggcaat aagttgctgc ttgtaaaagt
      181 actagagatg aaagatagta caaaaaaaga agatgcagca ggaatgtatg aaattatcag
      241 tgaaacacgg gtagaagaaa atgtctaaaa atattgtaca attgaataat tcttttattc
      301 aaaatgaata ccaacgtcgt cgctacctga tgaaagaacg acaaaaacgg aatcgtttta
      361 tgggaggggt attgattttg attatgctat tatttatctt gccaactttt aatttagcgc
      421 agagttatca gcaattactc caaagacgtc agcaattagc agacttgcaa actcagtatc
      481 aaactttgag tgatgaaaag gataaggaga cagcatttgc taccaagttg aaagatgaag
      541 attatgctgc taaatataca cgagcgaagt actattattc taagtcgagg gaaaaagttt
      601 atacgattcc tgacttgctt caaaggtgat aaaatggaaa atttattaga cgtaatagag
      661 caatttttga gtttgtcaga tgaaaagctg gaagaattgg ctgataaaaa tcaattattg
      721 cgtttacaag aagaaaagga aaggaagaat gcgtaaattc ttaattattt tgttgttacc
      781 aagttttttg accatttcaa aagtcgttag cacagaaaaa gaagtcgtct atacttcgaa
      841 agaaatttat tacctttcac aatctgactt tggtatttat tttagagaaa aattaagttc
      901 tcccatggtt tatggagagg ttcctgttta tgcgaatgaa gatttagtag tggaatctgg
      961 gaaattgact cccaaaacaa gttttcaaat aaccgagtgg cgcttaaata aacaaggaat
     1021 tccagtattt aagctatcaa atcatcaatt tatagctgcg gacaaacgat ttttatatga
     1081 tcaatcagag gtaactccaa caataaaaaa agtatggtta gaatctgact ttaaactgta
     1141 caatagtcct tatgatttaa aagaagtgaa atcatcctta tcagcttatt cgcaagtatc
     1201 aatcgacaag accatgtttg tagaaggaag agaatttcta catattgatc aggctggatg
     1261 ggtagctaaa gaatcaactt ctgaagaaga taatcggatg agtaaagttc aagaaatgtt
     1321 atctgaaaaa tatcagaaag attctttctc tatttatgtt aagcaactga ctactggaaa
     1381 agaagctggt atcaatcaag atgaaaagat gtatgcagcc agcgttttga aactctccta
     1441 tctctattat acgcaagaaa aaataaatga gggtctttat cagttagata cgactgtaaa
     1501 atacgtatct gcagtcaatg attttccagg ttcttataaa ccagagggaa gtggtagtct
     1561 tcctaaaaaa gaagataata aagaatattc tttaaaggat ttaattacga aagtatcaaa
     1621 agaatctgat aatgtagctc ataatctatt gggatattac atttcaaacc aatctgatgc
     1681 cacattcaaa tccaagatgt ctgccattat gggagatgat tgggatccaa aagaaaaatt
     1741 gatttcttct aagatggccg ggaagtttat ggaagctatt tataatcaaa atggatttgt
     1801 gctagagtct ttgactaaaa cagattttga tagtcagcga attgccaaag gtgtttctgt
     1861 taaagtagct cataaaattg gagatgcgga tgaatttaag catgatacgg gtgttgtcta
     1921 tgcagattct ccatttattc tttctatttt cactaagaat tctgattatg atacgatttc
     1981 taagatagcc aaggatgttt atgaggttct aaaatgaggg aaccagattt tttaaatcat
     2041 tttctcaaga agggatattt caaaaagcat gctaaggcgg ttctagctct ttctggtgga
     2101 ttagattcta tgtttctatt taaggtattg tctacttatc aaaaagagtt agagattgaa
     2161 ttgattctgg ctcatgtgaa tcataagcag agaattgaat cagattggga agaaaaggaa
     2221 ttaaggaagt tggctgctga agcagagctt cctatttata tcagcaattt ttcaggagaa
     2281 ttttcagaag cgcgtgcacg aaattttcgt tatgattttt ttcaagaggt tatgaaaaag
     2341 acaggtgcga cagctttagt cactgcccac catgctgatg atcaggtgga aacgattttg
     2401 atgcgcttga ttcgaggaac tcgcttgcgc tatctatcag gaattaagga gaagcaagta
     2461 gtcggagaga tagaaatcat tcgtcccttc ttgcattttc agaaaaaaga ctttccatca
     2521 atttttcact ttgaagatac atcaaatcag gagaatcatt attttcgaaa tcgtattcga
     2581 aactcttact taccagaatt ggaaaaagaa aatcctcgat ttagggatgc aatcttaggc
     2641 attggcaatg aaattttaga ttatgatttg gcaatagctg aattatctaa caatattaat
     2701 gtggaagatt tacagcagtt attttcttac tctgagtcta cacaaagagt tttacttcaa
     2761 acttatctga atcgttttcc agatttgaat cttacaaaag ctcagtttgc tgaagttcag
     2821 cagattttaa aatctaaaag ccagtatcgt catccgatta aaaatggcta tgaattgata
     2881 aaagagtacc aacagtttca gatttgtaaa atcagtccgc aggctgatga aaaggaagat
     2941 gaacttgtgt tacactatca aaatcaggta gcttatcaag gatatttatt ttcctttgga
     3001 cttccattag aaggtgaatc aattcaacaa atacctgttt cacgtgaaac atccatacac
     3061 attcgtcatc gaaaaacagg agatgttttg attcaaaatg ggcatagaaa aaaactcaga
     3121 cgtttattta ttgatttgaa aatccctatg gaaaagagaa actctgctct tattattgag
     3181 caatttggtg aaattgtctc aattttggga attgcgacca ataatttgag taaaaaaacg
     3241 aaaaatgata taatgaacac tgtactttat atagaaaaaa tagataggta aaaaatgtta
     3301 gaaaacgata ttaaaaaagt cctcgtttca cacgatgaaa ttacagaagc agctaaaaaa
     3361 ctaggtgctc aattaactaa agactatgca ggaaaaaatc caatcttagt tgggatttta
     3421 aaaggatcta ttccttttat ggctgaattg gtcaaacata ttgatacaca tattgaaatg
     3481 gacttcatga tggtttctag ctaccatggt ggaacagcaa gtagtggtgt tatcaatatt
     3541 aaacaagatg tgactcaaga tatcaaagga agacatgttc tatttgtaga agatatcatt
     3601 gatacaggtc aaactttgaa gaatttgcga gatatgttta aagaaagaga agcagcttct
     3661 gttaaaattg caaccttgtt ggataaacca gaaggacgtg ttgtagaaat tgaggcagac
     3721 tatacctgct ttactatccc aaatgagttt gtagtaggtt atggtttaga ctacaaagaa
     3781 aattatcgta atcttcctta tattggagta ttgaaagagg aagtgtattc aaattagaaa
     3841 gaataatctt taatgaaaaa acaaaataat ggtttaatta aaaatccttt tctatggtta
     3901 ttatttatct ttttccttgt gacaggattc cagtatttct attctgggaa taactcagga
     3961 ggaagtcagc aaatcaacta tactgagttg gtacaagaaa ttaccgatgg taatgtaaaa
     4021 gaattaactt accaaccaaa tggtagtgtt atcgaagttt ctggtgtcta taaaaatcct
     4081 aaaacaagta aagaaggaac aggcattcag tttttcacgc catctgttac taaggtagag
     4141 aaatttacca gcactattct tcctgcagat actaccgtat cagaattgca aaaacttgct
     4201 actgaccata aagcagaagt aactgttaag catgaaagtt caagtggtat atggattaat
     4261 ctactcgtat ccattgtgcc atttggaatt ctattcttct tcctattctc tatgatggga
     4321 aatatgggag gaggcaatgg ccgtaatcca atgagttttg gacgtagtaa ggctaaagca
     4381 gcaaataaag aagatattaa agtaagattt tcagatgttg ctggagctga ggaagaaaaa
     4441 caagaactag ttgaagttgt tgagttctta aaagatccaa aacgattcac aaaacttgga
     4501 gcccgtattc cagcaggtgt tcttttggag ggacctccgg ggacaggtaa gactttgctt
     4561 gctaaggcag tcgctggaga agcaggtgtt ccattcttta gtatctcagg ttctgacttt
     4621 gtagaaatgt ttgtcggagt tggagctagt cgtgttcgct ctctttttga ggatgccaaa
     4681 aaagcagcac cagctatcat ctttatcgat gaaattgatg ctgttggacg tcaacgtgga
     4741 gtcggtctcg gcggaggtaa tgacgaacgt gaacaaacct tgaaccaact tttgattgag
     4801 atggatggtt ttgagggaaa tgaagggatt atcgtcatcg ctgcgacaaa ccgttcagat
     4861 gtacttgatc ctgccctttt gcgtccagga cgttttgata gaaaagtatt ggttggccgt
     4921 cctgatgtta aaggtcgtga agcaatcttg aaagttcacg ctaagaacaa gcctttagca
     4981 gaagatgttg atttgaaatt agtggctcaa caaactccag gctttgttgg tgctgattta
     5041 gagaatgtct tgaatgaagc agctttagtt gctgctcgtc gcaataaatc gataattgat
     5101 gcttcagata ttgatgaagc agaagataga gttattgctg gaccttctaa gaaagataag
     5161 acagtttcac aaaaagaacg agaattggtt gcttaccatg aggcaggaca taccattgtt
     5221 ggtctagtct tgtcgaatgc tcgcgttgtc cataaggtta caattgtacc acgcggccgt
     5281 gcaggcggat acatgattgc acttcctaaa gaggatcaaa tgcttctatc taaagaagat
     5341 atgaaagagc aattggctgg cttaatgggt ggacgtgtag ctgaagaaat tatctttaat
     5401 gtccaaacta caggagcttc aaacgacttt gaacaagcga cacaaatggc gcgtgcaatg
     5461 gttacagagt acggtatgag tgaaaaactt ggcccagtac aatatgaagg aaaccatgct
     5521 atgtctggtg cacagagtcc tcaaaaatca atttcagaac aaacagctta tgaaattgat
     5581 gaagaggttc gttcattatt aaatgaggca cgaaataaag ctgctgaaat tattcagtca
     5641 aatcgtgaaa ctcacaagtt aattgcagaa gcattattga aatacgaaac attggatagt
     5701 acacaaatta aagctcttta cgaaacagga aagatgcctg aagcagtaga agaggaatct
     5761 catgcactat cctatgatga agtaaagtca aaaatgaatg acgaaaaata accctgagag
     5821 aggctggagc
//
LOCUS       DAVKGU010000100         5827 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKGU010000100 DAVKGU010000000
VERSION     DAVKGU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.101"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(58..957)
                     /locus_tag="VW077_001840"
     CDS             complement(58..957)
                     /locus_tag="VW077_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9455805.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(1029..1589)
                     /gene="rnmV"
                     /locus_tag="VW077_001841"
     CDS             complement(1029..1589)
                     /gene="rnmV"
                     /locus_tag="VW077_001841"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9455806.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(1589..2362)
                     /locus_tag="VW077_001842"
     CDS             complement(1589..2362)
                     /locus_tag="VW077_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9455807.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALADEMGVTQMNIVGFDKPTIE
                     HALKLVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(2667..3560)
                     /locus_tag="VW077_001843"
                     /pseudo
     CDS             complement(2667..3560)
                     /locus_tag="VW077_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(3844..3978)
                     /gene="rpmH"
                     /locus_tag="VW077_001844"
     CDS             complement(3844..3978)
                     /gene="rpmH"
                     /locus_tag="VW077_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9455808.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(4123..5337)
                     /locus_tag="VW077_001845"
     CDS             complement(4123..5337)
                     /locus_tag="VW077_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9455809.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(5493..5787)
                     /locus_tag="VW077_001846"
                     /pseudo
     CDS             complement(5493..5787)
                     /locus_tag="VW077_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1636 a         1346 c         1030 g         1815 t
ORIGIN      
        1 actttagaac tgttttgata cgaggacaag gacatttttg tcactgattc actatgttta
       61 taaatatttt ttgacatcct cttgccattc ctctgtcaat ttctttacta gaaattcatc
      121 tccaattagc ttcgcaaatg ttttagaaga ctgataaaat gattcagcta aactaaaatc
      181 tctttctact cttaatgctt gcttccacaa aaacataaat acaagaggtt tcttttgata
      241 atcttgagtt ttatccataa tttcttgtaa acacgataga taatcattaa aacaatcagt
      301 tactcctacc ttttcaagac aacacattcc tgaaataaga gtatctctaa ttataaagta
      361 attctctatt tgagttacct tcttctgttc caaaagaaag agcattagct tttcgatttg
      421 atttataaat ttggcaagat cttttctaat gagcatttga taaaaaaaca aacgaatcaa
      481 caacatatca tttattgaat aagcttcttt ttctataatc tgaggcatat gttcctcaag
      541 tattaactct gcatattcag gccttctagt tctagaagta tccaaacacg cctgcaatac
      601 ttcacatgct aattgttctt ctttaggaag attatcaaag tatgttttat aaatctcttc
      661 caaacacgct tccttcttat cgtactcctc ttctttacca tagattggtt cacgtaaaat
      721 ctggtatttc aattctaaat aagcagaagg aagagctgaa aaatccggca taaggctata
      781 aactggaact cctagccggc gagcaatata ggctaacttg ggcaaactcg gttgcgaagc
      841 tccactttca attcgcgaca gttgacgaac tgtcagttct tgctcatctc cacaaaaatc
      901 tggacgacta attccttttt ctatccgaat ctctttaatt ttttgaccaa ttaacatcag
      961 acgcctcctc ttctacctgc ttcgaaaaat actgtgaaac ttaaaaaatt gtaacggcta
     1021 catctttcct actcataaga tttcatagct tcttccactt ctgccaaagt aaccccaaac
     1081 aactctaggc gtttgaggag ttgcttgccg ttagaatagc cgattcggag agtctctccg
     1141 agatattctc tacgcttacg gctgtctgcc cctgctagaa aaccaaggcg aatcaagtcg
     1201 ctacgactaa tgtcaaactg actctcatgt tcaaattgtt ctgtcacctg agctagagcc
     1261 gttttcaggt cttcatagct ggcatgctca attcccagag aacgcccctt ggtcttggac
     1321 ttgggaacag cttcatctcg cttgagaaag gcatgctgaa ctgttggaat ggccgtcata
     1381 atcatgcgcc gaatccgctc cccattaaaa tctgggtctg taaagacaat gactccatga
     1441 cgttggtgta gacgctgaat ccgctctatg tcctggtcat tgatggcaga acctcgagtc
     1501 tcataggtct ccacatcgaa ataacgcttg agattgaccg tatcatcacg accttcaacc
     1561 acgataactt gggaaattct ctctttcatt acttgctgtc caatccaaaa attcgttctg
     1621 catttgcagt cgttgctacc gccagctctt ctgtcgtcat accacgcaag tcagcgataa
     1681 agtcgaccac atagcgagta taggctgttt tattttcacg accacgcttg ggtacaggtg
     1741 ctaagtaagg cgcatctgtt tccaccaaca tcttgtccaa aggtaactct ttagctgctt
     1801 cttggaggtc agttgccttc ttgaaagtca ccactcctga gaaggaaatg gtcataccaa
     1861 gatccacaaa cttctctgcc cactcaagcg tccctgaaaa tgaatgcatg ataccaccac
     1921 gaggaccaac gccctcactc ttgataatct cataggtatc ttccagcgca tcacgggtat
     1981 ggacaacaaa aggcaaatcc aagtccttag atagctgaat ctgacggcga aaaacctgct
     2041 cctgcacctc tttgggcgct gtcatccaat ggtagtctaa gccaatttca cctaaagcca
     2101 caaccttgga atgttttaac ttatccaaca agtaagcctc aacttcctct gtataagtac
     2161 cagcttctgt aggatgccaa ccaatagtcg catagagctg ctcatactca tctaccaact
     2221 tcaaggcatg ctcaatcgtc ggtttatcaa aaccaacaat attcatctgt gtcacaccca
     2281 tctcatcagc caaggcaatt tcttctgcct cacgacctgc aaattcttct acattcaagt
     2341 gtgtatgtgt atcaaaaatc atctcttcta acctcgtttt ctatcttcta ttataccaaa
     2401 aaagaggagg ggcacctaat ttttcggttt cccctcctct cttcaataga gagctattct
     2461 gctatctttt ctatccgata ttgcccatct cctgtcccac agttagagac agaagagatt
     2521 ggctacattg atagaatatt cctattcagc taaagtatgt gattatcttg ggttctcact
     2581 tttctcaccc atcactagga ttgataactt ctcaaaatcc cccttataat ctaactttaa
     2641 gtagagaata gaattttcta gctgttctat tttctacgtc tagtctttct tcctagcaaa
     2701 gcaaaaccac ttgttgccgc cgctagacca agactagcaa gtgctgcact tgatgctgaa
     2761 cctgtgttag gtaattgttt gccagcaggt gactggtagg taacatcttg cattgttcct
     2821 gcagttgctt gcggtgtact tgttgtctta ggagcagcat taggagccac tggtctctta
     2881 ggaactgtaa ccatggcaat ggcttgcact ccaatcgttt ctgcttcctt cacatcttcc
     2941 atagttttcg tattttcaat ctctttgaga gctgcttgtt tttctgcttc cactcttgct
     3001 aaaagttctg ctttttcttt atcagaaagc ggtgcgcctt tgatttcatc ctgcttgtcc
     3061 ttggctgctt tttcaatggc atttttagct gattcttttt cagtagctaa ttgcttagaa
     3121 gcttgtgatt gagctgagtt ttgtgaacct gtgtgagctg atccacccgc ctggctacca
     3181 ccgccttcat tcgcattagc atcttagctt ccagtattgc ctcccttatc tccacctggt
     3241 gtattttcta gcttatactc tggtgtagct tcttgagtta cagattcacc tttcgcagat
     3301 tgttgaactg tatcttttcc tagaatcgtc actactgaac catctgggaa tgtgattgtt
     3361 gctgtaccat ctccagctac attgattgtc gctccgtcta atgctgaacc atttgcttgt
     3421 aaaatagcaa ccttaacttt ttcttcatct gttaaatgag ttatatcttt tacatctact
     3481 ctttgagcag gtgctttaac agttgatttt tcagcagtca gtttagtgct atcttgtgct
     3541 tttggatctt gtgctaccaa ttgatttcct ggaattgtaa tagctgaacc atctttaaag
     3601 acgattgttg ctgtaccatc tccacctact tcaactttat agtaaaataa aataataaca
     3661 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaaa cagaggtgta
     3721 ctattctagt ttcaatctac tgtaacatcg cttccgaatc catttgcatc tactgaactt
     3781 ttttacaaaa ataaaaatca gtagactcga gttcctactg atttttgata gaattcgttt
     3841 ggattatgca gccaaaactt tgcgtccttt acgacgacga gctgccaata cgcgacgacc
     3901 atttttagtt gacatacggt tacggaatcc gtgtttgcgc gcacgacgaa gtttacttgg
     3961 ttgataagta cgtttcacga tgaatacctc ctcatagatt ttgtattcgt ttagccggct
     4021 ataaagtgat cagttactaa acatacttta ctattctatc tgattctttc acttttgtca
     4081 atagctctag gcaaatgttt ccagcttttt cgaatgcaag ccctatctac gatactgttt
     4141 caagaaacga gtcatctttt cttggatttg ggctaactca tcaacacgag gaaggtaaac
     4201 gatacggaag tggtctggtt cctgccagtt aaagcctcga ccatgaacca agagaacctt
     4261 ttcctgcttc aagaaatcaa ggacaaactg ctcatcatca tcgatacggt acatattgcg
     4321 gtcgattttt gggaagatat agagccccgc cttgggttta acggcagaca aacctggaat
     4381 atcttgaatg gcattataga tgaaatttct ttgctcgtag attcgtccac caggaagaag
     4441 caattcatcg actgattggt gaccccccaa ggaagtttgt acgacttgtt gggccaaaac
     4501 gttagagcaa aggcgcatat tggacagcat attgagccct tcgatatagc ccttaacatg
     4561 agtcttaggg ccagacaaga ccatccaacc cacacggaaa cctgctatgc ggtgggattt
     4621 tgacagacca ttcatgctga cacagaagac atctggtgcc aagctcgcca caggcgtatg
     4681 cacatgtccg tccattacca tgcggtcata gatttcatcc gcaaagataa tcaaatcgtt
     4741 ttgacgggca atctcaataa tctccagcaa gagttcctta ggataaaggg ctccagttgg
     4801 gttatttgga ttgataagga caattgcctt ggtattggaa gtgatttttg acttgatatc
     4861 gtcaatatct gggtaccatt ccacagcttc atcacagata tagtgaacgg catttcctcc
     4921 agctaggctg acagccgccg tccatagagg atagtctggc attggcacta agacctcatc
     4981 cccattgtcc aaaagcccct gcatggacat gacaatcaac tcactgacac catttccaag
     5041 gtaaatatca tcaatatcta cgtttgggaa tttcttcagc tggcaatact gcatgatggc
     5101 cttacgggct gagaaaatcc ctttggagtc agaatagcct tcactatcac gcgcattcat
     5161 aatcaagtca tgaatgacct cgtctggcgc tgtaaagcca aattctgctg gatttcctgt
     5221 attcagacgt aaaatctttt ctccattcgc tcgcatccgc atggcttctt ccaatacagg
     5281 accacggata tcataagcaa catgctctaa cttactagac ttgttatatt ctttcatctt
     5341 gtttcctcaa ttcatcagga tagtaacatt ataccactag aaagagaatg cgacaagttt
     5401 actgaaatgt gttactagtt tcgtatagaa gaaaaacctt gctaaaggcg aggttaagaa
     5461 cgtagagata gcaggtattt tgcaaagaca catcacatac caaaagataa acctagaaaa
     5521 aagcaagcct agaaaggagt cataaagaag attaaacatg aggaaaagcc cccatatcat
     5581 caagtaatcc caacgcccac ttcgttttgc aactagaaat aagattagat ggtagagtac
     5641 atgaaaaact aaaaatccaa taaaacttgg ataaagaagc ggtaccagac tctctaattg
     5701 acagatcatg atcacttcta ccaagactga aactaggata attccataat aaaggaaatt
     5761 tgatttttga aaagagtttt tcatcattct cccttccttc acttctttcc tgctatcctt
     5821 tctttta
//
LOCUS       DAVKGU010000101         5767 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKGU010000101 DAVKGU010000000
VERSION     DAVKGU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.102"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            58..453
                     /locus_tag="VW077_001847"
     CDS             58..453
                     /locus_tag="VW077_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9455810.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            464..835
                     /locus_tag="VW077_001848"
     CDS             464..835
                     /locus_tag="VW077_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9455811.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            845..3088
                     /locus_tag="VW077_001849"
     CDS             845..3088
                     /locus_tag="VW077_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9455812.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRVNGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QAAGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            3295..5070
                     /gene="spxB"
                     /locus_tag="VW077_001850"
     CDS             3295..5070
                     /gene="spxB"
                     /locus_tag="VW077_001850"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9455813.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            5181..5528
                     /locus_tag="VW077_001851"
     CDS             5181..5528
                     /locus_tag="VW077_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9455814.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(5645..>5767)
                     /locus_tag="VW077_001852"
     CDS             complement(5645..>5767)
                     /locus_tag="VW077_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9455815.1"
                     /translation="AFRTLEDVINQLQDVIQGLEKEVIKSIVNRRWTRMLFESR"
BASE COUNT         1703 a         1081 c         1324 g         1659 t
ORIGIN      
        1 attttaagag tatactgatg agtgtaattg acaaatgtag attttggagg tatggtaatg
       61 cagatttcag atgcagaatg gcaggtcatg aagattattt ggatgcaggg ggagcagacc
      121 agtacggatt tgattagggt tttggcagag cgatttgatt ggtccaagtc cacggttcag
      181 acacttttgg ctcggctggt tgagaaagag tgtttgactc ggaaaaaaga aggcaagtcc
      241 tttgtttatt cagccctttt aactctagac caaagtcggg atttacttgt ccaagatatc
      301 aaagacaagg tttgttcccg taggattagg aatttgttgg ctgacttgat tgtagaatgt
      361 gaatttactc agactgactt ggaagacttg gaagctgtga tttcagagaa gaaatcaagt
      421 gctgtaacag aagtaagatg taattgtatg taaaggagac gtcatgttaa atagtattgt
      481 aaccattatt tgtattgccc ttatcgcgtt tatcttgttt tggtttttca aaaagcctga
      541 aaaatctgga caaaaagccc agcaaaaaaa cggataccaa gagattcgag tggaagtcat
      601 gggaggctat actcctgagt tgattgtcct caagaaatca gtgccagcac gcattgtctt
      661 tgaccgcaag gatccttcac catgtctgga tcaaattgtt tttccagatt ttggtgtaca
      721 tacgaacctg ccaatggggg aagagtatgt agtggaaatc acgcctgagc aggctggaga
      781 gtttggcttt gcttgtggta tgaacatgat gcacggcaag atgattgtag agtaggtgga
      841 gactatgaca gaaattgtga aagcaagctt agaaaatggc attcaaaaaa tccgtatccg
      901 agctgaaaaa ggctatcatc cagcccatat ccagcttcaa aagggaattc cagctgagat
      961 tacctttcat cgtgctactc cttcaaactg ttataaggaa attctgtttg aagaagaagg
     1021 tatcttggaa ccaatcggcg tagatgagga gaaagtcatt cgttttacac ctcaagaatt
     1081 agggcgacat gaattttctt gtggcatgaa gatgcaaaag ggaagctata tagtcgttga
     1141 gaagactcga aaatctctat ctctcctgca acgtttttgg attactagta tctttactgt
     1201 gcctcttgtg attctcatga ttgggatgtt ggcaggtagc attagtcatc aagtcatgca
     1261 ttggggaacc tttttagcaa caacgcctat tatgttagtt gcaggtaagc catatatcca
     1321 gagtgcttgg gccagtttta aaaagcacaa tgccaacatg gataccttgg ttgcgctggg
     1381 aactctagtg gcttatttct atagcctagt tgctctcttt gctggtctcc ctgtttactt
     1441 cgaaagtgct ggatttatcc tctttttcgt tcttttggga gcagtttttg aggaaaaaat
     1501 gagaaaaaat acgtcccaag ctgtggagaa attactggac ttgcaagcta aaaccgcaga
     1561 agtcttgagt gatgatagtt atgtccaagt tcctttggaa caagtcaagg taggcgacct
     1621 gattcgagtg cgtcccggtg aaaagattgc tgttgatggt gtcgtagtag aaggtgtctc
     1681 tagtattgac gaatccatgg tgacaggtga gagtctgcct gtggacaaga cagttggaga
     1741 tactgtcatt ggctcaacca tcaatcatag tggaacgctt gtctttagag cagaaaaagt
     1801 tggctcagag actgttttgg ctcagattgt ggattttgtg aagaaagctc agacaagtcg
     1861 tgcgccgatt caggacttga cggataagat ttcagggatt tttgtcccag tagttgtcat
     1921 tttaggaatc atgacctttt gggtttggtt cgtcttgctc agggatagtg tggtcgtgct
     1981 tggagctagc tttgtgtcct ctcttctcta cggagtggcg gttttgatta tcgcctgtcc
     2041 ttgtgccttg ggacttgcaa caccgacagc ccttatggtg gggacaggac gtagtgccaa
     2101 gatgggggtt ctcctcaaaa atggaactgt cttacaggaa atccagaaag ttcaaactct
     2161 tgtctttgat aagaccggga ctttgacgga agggaaacct gtggtaacag atatcatcgg
     2221 cgacgaagta gaagtgtttg gattggcagc ctccttggaa gatgcttctc aacacccact
     2281 ggctgaggct atcgttaagc gagcgagtga agctggactt gagtttcaaa ctgttgaaaa
     2341 ttttcaggcc ttgcacggga aaggtgtttc agggcgagtc aatggaaaac aagttttact
     2401 tggaaatgct aaaatgctgg atggcatgga tatttctaat acttatcaag ataaactaga
     2461 agaactagaa aaagaagcta agacagttgt gtttttagct gttgacaatg aaatcaaagg
     2521 cttgcttgct ttgcaagata ttcctaagga aaatgctaag ctagccatca gtcagctaaa
     2581 aaaacgtggt ctccgaacag tcatgctgac aggagacaat gctggtgtgg cgcgtgctat
     2641 tgcagatcaa atcggaattg aagaggtcat tgcaggcgtc ttgccagaag aaaaagccca
     2701 tgaaatccat aaactgcaag cggctggcaa agtagccttt gttggggacg gtatcaatga
     2761 cgctcctgcc cttagtgtag cagatgtggg aattgctatg ggtgctggaa cagatatcgc
     2821 catcgagtca gcagatttgg tgttgacaac caataatctt ttaggagtgg ttcgtgcctt
     2881 tgatatgagt aagaaaacct ttcatcgaat tctactcaat cttttctggg cttttatcta
     2941 caatgttgtc ggaattccga ttgcagcagg agtcttttca ggtgttggac tggctctcaa
     3001 cccagaattg gcaggtctag ccatggcctt tagttctgta tctgttctga ccagttccct
     3061 tctgttaaat tttagtaaaa tagactaaaa gtaatgacta gatttctttg ttataaaaca
     3121 gaaatgacaa cactttcaaa actgtattag aatagagaat agagagtttt gagcagattt
     3181 ttagaaaagt cagcataaat atgatacagt ggaatagtaa aaatttggag aacgtttcca
     3241 attctatgta atcgtattct ccaagtttaa aaaaattgaa ggagagttat cattatgact
     3301 caagggaaaa ttactgcatc tgcagcaatg cttaacgtat tgaaaacatg gggcgtagat
     3361 acaatctacg gtatcccatc aggaacactc agctcattga tggatgcttt ggctgaagac
     3421 aaagatatcc gtttcttaca agttcgccac gaagagacag gtgctcttgc agcggttatg
     3481 caagctaaat tcggcggctc aatcggggtt gcagttggtt caggtggtcc aggtgcgact
     3541 cacttgatta acggtgttta cgatgcagct atggataaca ctccattcct agcgatcctt
     3601 ggatcacgtc cagttaacga attgaacatg gatgctttcc aagagcttaa tcaaaaccca
     3661 atgtacaacg gtatcgctgt ttacaacaaa cgtgtagctt acgctgagca attgccaaaa
     3721 gtaattgacg aagcctgccg tgctgcagtt tctaaaaaag gtccagctgt tgttgaaatt
     3781 ccagtaaact tcggtttcca agaaatcgat gaaaactcat actacggttc aggttcatac
     3841 gaacgctcat tcatcgctcc tgctttgaac gaagttgaaa tcgacaaagc tgttgaaatc
     3901 ttgaacaatg ctgaacgccc agttatctat gctggatttg gtggtgttaa agctggtgaa
     3961 gtgattactg aattgtcacg taaaatcaaa gcaccaatca tcacaactgg taaaaacttt
     4021 gaagctttcg aatggaacta tgaaggtttg acaggttctg cttaccgtgt tggttggaaa
     4081 ccagccaacg aagtggtctt tgaagcagac acagttcttt tccttggttc aaacttccca
     4141 ttcgctgaag tttacgaagc attcaagaac actgaaaaat ttatccaagt cgatatcgac
     4201 ccttacaaac ttggtaaacg tcatgctctt gacgcttcaa tccttggtga tgctggtcaa
     4261 gcagctaaag ctatccttga caaagtaaac ccagttgaat caactccatg gtggcgtgca
     4321 aacgttaaga acaaccaaaa ctggcgtgat tacatgaaca aactcgaagg taaaactgag
     4381 ggtgaattgc aattgtatca agtttacaat gcaatcaaca aacatgctga tcaagacgct
     4441 atctactcaa tcgacgtagg taacactact caaacatcta ctcgtcacct tcacatgaca
     4501 cctaagaaca tgtggcgtac atctccactc tttgcgacaa tgggtattgc ccttcctggt
     4561 ggtatcgctg ctaagaaaga caatccagat cgccaagtat ggaacatcat gggtgacgga
     4621 gcattcaaca tgtgctaccc agacgttatc acaaacgttc aatacgacct tccagttatc
     4681 aaccttgtct tctcaaatgc tgagtacggc ttcatcaaga acaaatacga agatacaaac
     4741 aaacacttgt ttggtgtaga cttcacaaac gctgactacg ctaaaattgc ggaagctcaa
     4801 ggagctgttg gattcacagt tgaccgtatc gaagacatcg atgcagttgt tgcagaagct
     4861 gttaaattga acaaagaagg taaaactgtt gtcatcgatg ctcgcatcac tcaacaccgt
     4921 ccacttccag tagaagtact tgaattggat ccaaaacttc actcagaaga agctatcaaa
     4981 gccttcaagg aaaaatacga ggcagaagaa ctcgtaccat tccgtctctt cttggaagaa
     5041 gaaggattgc aatcacgcgc aattaaataa ttcctctcgc cgaaaatcaa atatgaaact
     5101 tgtagacttt attctttgat tttcatagag cagaacttta aaagatcgga gcaatccggt
     5161 ctttttatgg aggagagtaa atgaatttaa atcaattaga tattatcgtt tcaaatgttc
     5221 cccaagtctg tgctgacttg gagcatatct tggataaaaa ggcagattat gctgatgatg
     5281 gttttgctca gtttacgatt ggtagtcact gccttatgtt gtcacaaaat catttggttc
     5341 ctttggaaaa ctttcagtca ggaatcatta ttcatatcga ggttgaggat gtagaccaga
     5401 actacaaacg gttgaacgag cttggtataa aggttttaca cggaccaact gtaaccgatt
     5461 ggggaacaga gtccttatta gttcaaggac ctgctggtct agtgcttgat ttttatcgta
     5521 tgaaataggt cgcgtagtca tttgacgtta accttagcta tttttaaaat agaaattgag
     5581 aatatgaact attgaaagat cggagcaatc cggtcttttt atataaagaa attgcatata
     5641 atactcatct gctttcaaaa agcattctag tccatctccg attaacgatg gactttatca
     5701 cctccttctc cagtccttgt atgacatctt gaagttgatt aatgacatct tccaaagttc
     5761 gaaaggc
//
LOCUS       DAVKGU010000102         5714 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKGU010000102 DAVKGU010000000
VERSION     DAVKGU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.103"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..270
                     /locus_tag="VW077_001853"
     CDS             <1..270
                     /locus_tag="VW077_001853"
                     /inference="COORDINATES: protein motif:HMM:NF019216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455816.1"
                     /translation="ERIAKFNNLISVSFNNPNVSWGRTNRITITSIANLQNMINTAVN
                     EDAEDFRAQLYPDTNSRVLKLKKAIYKAYLDQTNDFRRSIFENKK"
     gene            314..>5714
                     /locus_tag="VW077_001854"
     CDS             314..>5714
                     /locus_tag="VW077_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9455817.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSIFLGSFLFALSVVGVSTYHYLDYS
                     TLTQTERDQLKQGRPDESKESYALVYEKDALPNTGSSQSIMTVLGLLAIGSLIVIITK
                     DKKRKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDR
                     TVSSVSPASTVSPVEQPKVVTDKGEPEVQPALPEAVVTGKGKTEVQPTLPEAVVTEKG
                     EPEVQPVLPEAVVAEKGEPEVQPVLPEAVVTEKGEPEVQPALPEAVVTEKGEPEVHEK
                     PDYTQPIGANLVESEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEPISTVSEV
                     ASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDNSVKQVGEDGERQIVTSYEELHG
                     KKISESVETVTILKEMKPEIIVKGTKERPKEKTAPVLTLTKVTEDAMNRSANLNYELD
                     NKDNAEISSIVAEIKDGDTVVKRVDLSKEKLTDAVQNLDLFKDYKIATTMIYDRGQGS
                     ETSKLDERPLRLELKKVEIKNIASTNLVKVNDDGTETPSDFMNEKPSEEDVKKMYLKI
                     TSRDNKVTRLTVDSIEEVTEEGQKLYKITAEAQDLIQHTDPTKVRNKYVYYIEKPHPK
                     EDNVYYNFKDLVDAMNTDKNGTFKLGADLNATGVPTPKKWYVDGDFRGTLKSVEGKHY
                     TIHNTERPLFQNIIGGTVTKVNLGNVNINMPWADRIAPIADTIKGGAKIEDVKVTGNV
                     LGRNWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVERAYVDAN
                     LIGKKAKAAGIAYWTQNSGDNHKVGVEGAVKKGIVKGTIQVESPVEVGGAVGRLSHHG
                     YIGEVVSMMKVKKGEIFYGSSDMNDDPYWVANNVRGNYVVNGVSEGTISYARAKEHHR
                     IKPISQSEADTKIMMLGITAQDFAVNEPVVNRLNRLTRKEDEYKSTQDYKVDRDLAYR
                     NIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYA
                     DGTKEEMDVTKNADSKVKQVREYTIAGQNVVYTPNMVEKDRNQLIQDIKDKLASVQLI
                     SPEVRALMDARKKPEENTDERKNGYIKDLYLEESFAETKANLDKLVKSLVENADHQLN
                     SDEAAMKALVKKVDENKAKIMMALTYLNRYYDIKYGDMTIKNLMMFKPDFYGKSVDLL
                     DFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFTNDTDLNDWFIH
                     AAKNVYVSEPQTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAHMFLISTYNTMA
                     YSSFEKYGKYTEEARNEFKKEIDKVAHAQQTYLDFWSRLALPSVRDQLLKSENRVPTP
                     VWDNQNYSGIKGINRMGYDEKKVPIAPIRELYGPTWKFHNTNWNMGAMASIFPNPNNN
                     DQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAYAQGMLQTPSSI
                     GHQGEYGALGLNMAYHRENDGDQWYNYDPDKLQTREDIDRYMKNYNEALMMLDHVEAD
                     AVLPQLNGDNSKWFKKIDREMRRNLGDGLNNLVAPHQWDNVRDLNQEESSKKLSSIND
                     LIDNNFMTKHGNPGNGRYRPEDFRPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAF
                     RMWGYYGYDKGFTSYVSSKYQGEADKQNQGRLGDDFIIKKVSNNQFSNLEDWKKYWYH
                     DVKSRAEKGFTEITIDGQTIHNYNELKDLFDKAVTEDLKKAGNYSNTENLKSKVFKAL
                     LKNTDGFFNPLFKKDI"
BASE COUNT         2039 a          882 c         1195 g         1598 t
ORIGIN      
        1 gagcgaatag ctaaatttaa taatctaata agtgttagct ttaataatcc aaatgtaagt
       61 tggggaagaa ctaatagaat aacaataact agcatagcca accttcaaaa tatgataaat
      121 acggctgtca atgaagatgc tgaagatttt agagcacaat tataccctga tacaaatagt
      181 agagttctta aactgaaaaa agcaatatat aaagcctatc ttgaccaaac aaatgatttt
      241 agaagatcaa tttttgagaa taaaaaatag tgtttactat taggaaataa aaagtgaatg
      301 tggaggatta gaattgtcat tattaaaaaa agataaattt tccattagga aaataaaggg
      361 tattgttggt tcgatattct tggggagttt tttatttgca ctgtcagttg ttggtgtatc
      421 gacttatcat tacttagatt atagtacttt gacacaaact gaacgtgatc aactcaaaca
      481 aggtagacct gatgaatcaa aagagtcata tgctttggtt tatgaaaaag acgcattacc
      541 aaacacagga agttctcaat ctattatgac tgtattgggt ttgttagcta tcgggagtct
      601 tattgtgatt atcacaaaag ataaaaagag gaaaaaaata gctacatttt tgattgtagg
      661 ggcgacaggt ttagttacac tatcaactgc ttcagcactt aatttgaatg ctaacatcca
      721 tgagtctgga cgtgatggtg tgttgcaaat ttctggttac agatatgttg gttacttgga
      781 gcttgatgat agaactgttt cgtcggtttc tccagcatca actgtttctc cagttgagca
      841 acccaaagtt gtgactgaca aaggcgaacc agaagttcag ccagcgttac cagaagcagt
      901 tgtaactgga aaagggaaaa ctgaagtaca accgacatta ccagaagctg ttgtgactga
      961 aaaaggggaa ccggaagtac aaccagtgtt accagaagct gttgtggctg aaaaagggga
     1021 accggaagta caaccagtgt taccagaagc tgttgtgact gaaaaagggg aaccagaagt
     1081 acaaccagcc ttacccgaag cagttgtaac tgaaaaaggc gaaccagaag ttcatgagaa
     1141 gcctgactat acgcaaccaa taggtgcaaa ccttgttgag tctgaagttc atgagaaact
     1201 agcatacact gaacctgtag gtacaacagg agtagatgaa aatggaaact tgattgagcc
     1261 acctgttaac gatattccag agtatactga accaatatct acagtttcag aagttgcatc
     1321 agaaagagaa gagttgcctt ctttacatac cgatattcgt acagagacta ttcctaaaac
     1381 cactatagaa gaatctgatc ctagcaaatt tataggcgat aattctgtta aacaagttgg
     1441 tgaggatggc gaacgtcaaa ttgttactag ctatgaagag ttacatggca aaaaaatcag
     1501 cgagtcagtt gaaacagtaa caattttgaa agaaatgaag cctgaaatta ttgtaaaagg
     1561 tacaaaagaa agaccaaaag aaaaaacggc tcctgtttta accttgacca aagtaacaga
     1621 agatgctatg aatcgttctg ccaacttaaa ttatgagctt gataataaag ataatgctga
     1681 aatatcaagt attgtagctg aaattaaaga tggtgataca gtagttaaaa gggttgattt
     1741 atctaaagaa aaattaactg acgctgtaca aaatttggat ttgtttaaag attataagat
     1801 tgcaacaaca atgatttatg atcgtggtca aggttctgaa acgtctaaac tggatgaaag
     1861 acctctgcgt ttggagttga aaaaggtcga aattaaaaat atcgcatcta ctaatttagt
     1921 gaaggtaaat gatgatggta ctgaaacacc tagtgatttt atgaatgaaa aaccttcaga
     1981 agaagatgtt aaaaaaatgt acttaaaaat cactagtcgc gataataagg taactcgttt
     2041 aactgtagac tctattgaag aagtgactga agaaggtcaa aaactttaca aaataactgc
     2101 tgaagcacaa gatttgattc aacacacaga cccaacgaag gttcgtaata agtatgttta
     2161 ttatatagaa aaaccccatc ctaaagaaga taatgtctac tataacttta aagatttagt
     2221 agatgctatg aatactgata aaaatggtac atttaaactt ggtgcagatt tgaatgccac
     2281 tggtgttcca acacctaaga aatggtatgt tgacggcgac tttagaggta cattgaagag
     2341 tgtagagggg aaacattata ctatccacaa cacggagcgt ccgttattcc agaatattat
     2401 tggtggtaca gtaaccaaag taaatctggg aaatgtaaat atcaatatgc catgggcaga
     2461 ccgcatcgca ccgattgctg acactattaa aggtggcgct aaaattgaag atgtcaaagt
     2521 gactggtaac gtattaggta gaaactgggt atctggattt attgataaga ttgacaacca
     2581 aggtacactc agaaatgtag cctttatcgg aaatgtgaca tctgtaggtg atggtggtca
     2641 attcttgacc ggtatcgttg gtgagaactg gaaaggacta gtcgaacgtg cgtatgttga
     2701 cgcaaatctt attggcaaga aagccaaggc tgctggtatt gcttattgga cacaaaacag
     2761 tggtgacaat cataaagttg gtgtcgaagg tgcagtcaag aaaggaattg tgaagggtac
     2821 cattcaggta gaaagtcctg ttgaagtcgg cggtgctgtt ggacgtttga gtcatcatgg
     2881 ttatatcggg gaagtcgtat ctatgatgaa agttaagaag ggtgaaattt tttatggttc
     2941 tagtgatatg aatgatgacc catattgggt ggcgaataat gtacgaggaa attatgtagt
     3001 taatggtgtg agtgaaggta cgatatctta tgctcgtgct aaagaacacc atcgtatcaa
     3061 accaatttca cagtcagaag cagatactaa aattatgatg ttaggtatta cagcacaaga
     3121 ttttgctgtc aacgaacctg tggtcaatcg cttaaaccgt ttaaccagaa aagaagatga
     3181 atataagtca acccaagact ataaagtaga tcgagatctg gcttatcgta atattgagaa
     3241 acttcaacca ttttataaca aagaatggat tgtcaaccaa ggtaacaaac ttgcagaaga
     3301 ttcaaactta gctaagaaag aagtcctttc tgttacgggt atgaaggatg gacagtttgt
     3361 aactgactta tcagatattg ataagattat ggttcactat gctgatggta ctaaagaaga
     3421 aatggacgtc actaagaatg ctgattctaa agtgaaacaa gttcgtgaat ataccatcgc
     3481 tggacaaaac gttgtttata caccaaatat ggttgagaaa gaccgtaacc aattgattca
     3541 agatatcaaa gataagttgg cgtcagttca attgatttct cctgaagtac gtgctttgat
     3601 ggatgcacgt aagaaaccag aagaaaatac tgatgaacgt aagaatggtt atattaaaga
     3661 cctttactta gaagaaagct ttgctgaaac gaaagctaat cttgataaat tggttaaatc
     3721 cttagttgaa aatgcagatc atcaattaaa tagtgacgaa gcggctatga aagcacttgt
     3781 taagaaggtg gatgaaaata aagctaaaat catgatggct ctgacttatc tcaatcgtta
     3841 ctatgatatc aagtatgggg atatgactat caagaacttg atgatgttta aacctgattt
     3901 ctatggtaaa tcagttgacc ttcttgattt cttgattcga attggatcaa gtgagagaaa
     3961 tatcaaaggt gacagaactt tggatgctta tcgtgatatg attggtggaa ctattggtaa
     4021 gtctgaatta catggattct tagattacaa tatgcgtctc ttcactaacg atactgattt
     4081 aaatgattgg ttcatccatg cagctaagaa tgtatatgta tcagaacctc aaacaacgaa
     4141 cccagatttt gttaataaac gacatcgtgc atttgatggt ttgaacaacg gtgttcataa
     4201 tcgtatgatt ctacctctat taactcttaa gaatgcacat atgttcttga tttcaactta
     4261 caatacaatg gcttatagtt cttttgagaa atatggtaag tatacagaag aagctagaaa
     4321 tgaattcaaa aaagaaattg ataaggttgc acatgctcaa caaacatact tagacttctg
     4381 gtcacgttta gcattgccaa gcgtacgtga ccaattactc aagagtgaga atagggtacc
     4441 aactcctgtg tgggataacc aaaactatag tggtattaaa ggaataaacc gtatgggtta
     4501 tgatgagaag aaagtaccaa ttgcgccaat tcgtgagtta tatggtccaa cttggaaatt
     4561 ccataatacc aactggaata tgggagctat ggcatctatt ttcccaaatc ctaataacaa
     4621 tgaccaagtt tatttcatgg gaacaaatat gattagtccg tttggtattt cagcctttac
     4681 tcatgaaact acacacgtta atgatagaat gctttacttc ggtggtcata gacaccgtca
     4741 aggtacagac gtcgaagcct atgctcaagg tatgctacaa actccgtcaa gtattggtca
     4801 ccaaggtgaa tatggtgctt taggtttgaa catggcttat catcgtgaaa atgatggtga
     4861 ccaatggtat aactatgacc ctgataagct tcaaactcgt gaagatatcg atcgatatat
     4921 gaaaaactat aatgaagcac tgatgatgct agatcatgta gaagcagacg ccgtacttcc
     4981 acaattgaat ggtgataata gcaagtggtt caagaaaatt gatagagaaa tgcgcagaaa
     5041 tcttggggat ggtttgaata acttagtagc accgcaccaa tgggacaacg ttcgtgactt
     5101 aaatcaagaa gagagttcta agaaattatc aagtattaac gatttgattg ataataattt
     5161 catgaccaaa cacggaaatc caggtaacgg acgttataga ccagaggatt ttagaccaaa
     5221 ctcagcttac gtaaatgtca atatgatggc aggaatttac ggtgggaata cgagtcaagg
     5281 tgctcctggt tcattatcgt tcaaacataa tgccttccgt atgtggggtt attatggata
     5341 tgacaaaggt ttcactagtt atgtatctag caaatatcaa ggtgaagctg ataaacaaaa
     5401 ccagggtaga ttaggtgatg atttcattat caaaaaagtt tcaaacaacc aattctcaaa
     5461 tcttgaagat tggaagaaat attggtatca tgatgtgaaa tccagagctg aaaaaggatt
     5521 cacagagatt acaattgatg gacaaaccat tcacaactat aatgaactga aagacttatt
     5581 cgacaaagct gtcacagaag acctcaagaa agctggaaac tactcaaata ctgagaattt
     5641 gaaatccaaa gtcttcaaag ctcttcttaa aaacacagac ggtttcttta atccattgtt
     5701 caaaaaagac attt
//
LOCUS       DAVKGU010000103         5613 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKGU010000103 DAVKGU010000000
VERSION     DAVKGU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.104"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..192
                     /locus_tag="VW077_001855"
     CDS             <1..192
                     /locus_tag="VW077_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9455818.1"
                     /translation="LKPLIAEDKAYNHALSKERSKVENIFAKVKTFKMFSTTYRNHRK
                     RFGLRMNLIAVIINHELGF"
     gene            260..508
                     /locus_tag="VW077_001856"
     CDS             260..508
                     /locus_tag="VW077_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455819.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            639..1811
                     /locus_tag="VW077_001857"
     CDS             639..1811
                     /locus_tag="VW077_001857"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9455820.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1811..3085
                     /locus_tag="VW077_001858"
     CDS             1811..3085
                     /locus_tag="VW077_001858"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9455821.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMAILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VVLSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVEQLIADKTFNLETAQRYLENLRS"
     gene            complement(3120..4085)
                     /locus_tag="VW077_001859"
     CDS             complement(3120..4085)
                     /locus_tag="VW077_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9455822.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(4066..5520)
                     /locus_tag="VW077_001860"
     CDS             complement(4066..5520)
                     /locus_tag="VW077_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9455823.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGNLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSHAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
BASE COUNT         1600 a         1132 c         1274 g         1607 t
ORIGIN      
        1 ctcaagccgt taatagctga agataaagct tataaccatg cgctatctaa ggagagaagc
       61 aaggttgaga acatctttgc caaagtaaaa acgtttaaaa tgttttcaac aacctatcga
      121 aatcatcgta aacgcttcgg attacgaatg aatttgattg ctgttattat caatcatgaa
      181 ctaggattct agttttgcag gaagtctact attttcctta ttgtctgtaa gtctactgac
      241 cttgttgttt atcccagtca tggtttctag ttcgggctca gagtttcaaa gtggatggca
      301 agagcatcaa ttgattgctg agaaggttag taaaacactt gataagacat ttgataagga
      361 tgtcagagaa attccgacca gtcagtttta tcaaaaattt gtagatgaga tgggaaggac
      421 ttactcagga aatttgatcc tccaggagct gataactgtg aatggagctt ataaagctac
      481 ttatatcggt gaactctcta gcaactaatc attctttaaa atgatgacca ataagaggct
      541 tgatttggtg aatcaagtct tttttctctc ttttactgcc atttgtgata aaatagtata
      601 gaacgatttt ttacatgaat gataaaacag aggtaaatat gacaatcggt attgataaga
      661 ttggttttgc gaccagtcaa tatgtcttga aattacaaga cttagcagaa gcgaggggaa
      721 ttgaccctga aaaattaagt aaaggactct tactcaagga attgagtatt gcgcccctaa
      781 ctgaggatat cgtgaccttg gcggccagtg ctagtgactc tattttaact gagcaagaaa
      841 gacaagaagt tgacatggtc attgtggcga ccgagtcagg aattgaccag agtaaggctg
      901 cggccgtctt tgtgcatggc ttgctgggca tccagccctt tgctcgtagt ttcgagatta
      961 aagaagcctg ctacggagcg actgctgccc tccattatgc caaattgcat gtggaaaatt
     1021 ctccggagtc caaggtcttg gtcattgcca gtgatattgc caaatacggt attgaaactc
     1081 caggagaacc aactcaaggt gctggaagtg tagctatgtt gattacacaa aatccacgca
     1141 tgatggcctt taataatgac aatgtagctc agacccgtga catcatggat ttctggcgac
     1201 caaattactc gacaactcct tatgtaaatg gtgtctattc tacccaacaa tacttggata
     1261 gtttgaaaac gacttggctt gaatatcaaa aacgctacca gcttactttg gatgattttg
     1321 cggctgtttg tttccacttg ccttatccta aattagcgct aaaaggcttg aaaaaaatca
     1381 tggataagag cctgcctcaa gagaaaaaag acctcttaca aaagcatttt gaccagtcta
     1441 ttctctacag tcaaaaggtg gggaatatct acacaggttc acttttcctt ggacttttgt
     1501 ctctcttgga aaatacagat agcttgaaag ctggggataa aatcgccctt tatagttacg
     1561 gaagtggagc tgtggctgag ttcttcagtg gtgaattggt tgaaggatat gaagcttatt
     1621 tggataaaga ccgcttgaac aagctcaacc aacgaactgc cctatccgtt gcagactatg
     1681 aaaaggtctt ttttgaggaa gtaaacttgg atgaaacaaa ctctgcccag tttgctggct
     1741 atgaaaatca agattttgcc ttggttgaaa ttctcgacca ccaacgccgt tatagcaagg
     1801 ttgaaaaata atgaagataa gttggaatgg attttctaaa aaatcatacc aagagcgcct
     1861 cgagctgtta aaagctcagg cgctccttag tcctgagaga caagctagtc tggagaagga
     1921 tgaacagatg agcgtgactg tggcagacca gctgagtgag aatgtagtgg gaactttttc
     1981 tctgccttat tcactggttc cggaggtact tgtcaacggt caggaataca ccgttcccta
     2041 tgtgacagaa gaaccctctg tggttgcggc ggccagctat gccagcaaaa tcatcaagcg
     2101 tgcaggtggt tttactgcac aagtccatca gcgacagatg attgggcagg tagcccttta
     2161 tcaagttgct aatcctaaac tagcgcaaga gaagattgcc agcaagaaag cggagctctt
     2221 ggagcttgcc aatcaagcct atccttctat cgttaaacgt ggaggtgggg cgcgtgatct
     2281 gcatgtcgag cagataaaag gtgaaccaga ctttctcgtt gtttatattc atgtcgatac
     2341 ccaggaagcc atgggtgcca atatgctcaa caccatgctg gaagccttga aaccagtctt
     2401 agaagaactc agtcagggac agagtctcat ggcaatcctg tccaactacg cgaccgattc
     2461 tctggtgact gcaagctgtc gcatcgcctt tcgctacttg agccgccaaa aggatcaagg
     2521 acgagagatt gcggagaaaa ttgcgttggc tagtcagttt gcgcaggctg atccttaccg
     2581 agctgctact cataataaag gaatttttaa tggtattgat gcgattttga ttgccactgg
     2641 taatgactgg cgtgccatcg aagctggggc ccatgccttt gccagtcgag atggacgtta
     2701 tcaaggtctt agttgctgga cgctggacct tgaaagagaa gaattggtcg gtgagatgac
     2761 cctgcccatg cctgtagcga ctaagggtgg ctctatcggc ctcaacccac gtgtagttct
     2821 cagtcatgat ctactaggaa atccttctgc cagagaatta gcccagatta tcgtgtccat
     2881 cggtcttgct caaaattttg cagccctcaa agccttggta agtacgggca tccagcaagg
     2941 ccacatgaaa ctacaggcca aatccctagc tctcctagct ggggctagtg aatctgaagt
     3001 tgctccccta gtcgagcagc ttatcgcaga taaaaccttt aacctagaga cagcccagcg
     3061 ctatctcgaa aatttaagat cataaaaact cagacgaatc ggtctgagtt ttcttgtgtt
     3121 taaatacttt ttcctggtaa tagcgtaact ggtaagaagt aaccagttgt ggcgacttcc
     3181 ttgccttcaa tcttttgcaa gagaagatca atagtgagac aagcaatctc ttccaaaggt
     3241 tgcttgatag tagccaattg agggtagtaa ttttcgataa agtaggtccc atcatagccg
     3301 atgaccttga gctcttttgg gacagaaatg cccaattctt gagcgatttt aatgaccaga
     3361 atagctgtca aatcatccga agcaaaaatg gcatctggtt tttcccgggt caagatattc
     3421 ttgatttcca tttcttttct gacgggagaa aagtcactgg aaacattgat aataggagct
     3481 tttgggagta cggatgcaaa accagcgtgg cgcagtccgg ttggcgaatt agaattgtca
     3541 ttccctgtaa tcatgatgat agactgggca cctgtcttga ccaaggtttg ggcagcaaga
     3601 accccaccag catagttgtc agaggagacg acagggatgt ctggcgatag gtttcggtca
     3661 aaggaaataa tcggcgctgt cacacgattg tagtcttcga ttcctaggtt gtgactacca
     3721 gaaatgatgc cgtccacctg attggcttcc aacatttcga tgtattcacg ttccttctca
     3781 gaatcatgtt cactgttgca gatgatggtc ttgtaaccat ttttgaagag ttggtgttcc
     3841 aatttatcaa tcaattctgc atagaaaaca ttggaaatat tggggaaaat caagccgatt
     3901 aacttagctg attttccttg cagactacga gccaggttgt tgggtttata gcctaattct
     3961 ctcatggctt cattgacttt ttggatggtt ttctcagata gatatccttt tttattgata
     4021 acccgagaaa cggtagtagg actgacgcct gcaagtttgg cgacatcagt tagttttgcg
     4081 accataatct aattcatagt aagttccagt tgggtttcca gatttgatca ggataccatt
     4141 ttggtccgca tgtgggaaga cacgaccaga aaatactttt tctcctttat tgatgaaaat
     4201 ttcaaagaca gagttatcga tgaagattgt agcagtagta gcctgattct cgatagggca
     4261 agaacgagtt gtcccaaatt cttgggcata ctgttctcca gcatggctac gatccactgt
     4321 cacttgaccg tttacaaggt caaagttgat tgaaagtccc ttgccttctt tatcagcaag
     4381 taagacaatc tcgctctggc tattggcttc caagttgagt tcaagttcgt aagtgttctt
     4441 ggtttgggaa cggtttgaga aggcttcttc agaagcacga aggtccttaa tagcagcgac
     4501 tggatactgg tagagcttgt cgtctttgat agtgagttcc ttgaccaaag agaaggttcc
     4561 ttggtggtca aaacggtcag atgggtaaga aacatctggc aaaccaagcc agctaactgc
     4621 tagagcacgc ccatcaggag cgttgaaggc ttgagttgca taggcttcga aaccgtaatc
     4681 catgttttga agttgagaca catctaccat tttggcattt ttagggtcaa aggaagcccc
     4741 aatcttatac atatttggaa agatattatc gtagtctaga actttcttat ccaatccttg
     4801 tggacagtag agaaggacag gctgttcctc tacaaagacc agattaggac attccatcat
     4861 gtaggcagta cggtcgttag caaagtcaag gttgccaact gcttgccagt ttgtgtagtc
     4921 gttattgaca gccttgtaga gacgaacgaa accttttttc tccaagtctt gtccgccgac
     4981 aatggcataa tattgaccct gaaagttaaa aatttgtgga tcgcggaagt ggtcagtaga
     5041 gtctgctggc tggtcaatca agatcttgtc aatctttgta atcttaccct ccttgtccat
     5101 caaagcaccg atttggtatg ggtgacggat ccagttttca tcgcgaacat ttcctgtata
     5161 aaataggaat aagttatcgc caaattgcat ggcagaacca gagtaggcac cgtgactatc
     5221 taatggagta tctggcaaaa ctttgactcc agtttctcta aagtgaacca aatcatcact
     5281 ttctagctgt gcccaagatt ttaaaccgtg ggctgcacca aaaggaaaat tctggtaaaa
     5341 gaggatccac ttgccatcaa agtaagaaaa gccatttggg tcgttgagaa gtcctgtttt
     5401 tggctcaaca tggtaatgag tatgccatgg agattgtgcc atattttcct ttatatgttg
     5461 aatttcttct tgcgtccaat cttgataaag tctgtaacga cgctcagttg tccattccat
     5521 tcattcctcc aaaaatctta tcacttttaa tagtaactgt tttcgataaa aaatgcaagc
     5581 cttttatatt aaaaaataga aaaaactgtc aaa
//
LOCUS       DAVKGU010000104         5519 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKGU010000104 DAVKGU010000000
VERSION     DAVKGU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.105"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(1..548)
                     /locus_tag="VW077_001861"
                     /pseudo
     CDS             complement(1..548)
                     /locus_tag="VW077_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            999..1574
                     /locus_tag="VW077_001862"
                     /pseudo
     CDS             999..1574
                     /locus_tag="VW077_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1571..1789
                     /locus_tag="VW077_001863"
     CDS             1571..1789
                     /locus_tag="VW077_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455824.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREVFKKMNSTWTINESMVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(1846..2130)
                     /locus_tag="VW077_001864"
     CDS             complement(1846..2130)
                     /locus_tag="VW077_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9455825.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(2184..3119)
                     /locus_tag="VW077_001865"
     CDS             complement(2184..3119)
                     /locus_tag="VW077_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9455826.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3223..3666
                     /locus_tag="VW077_001866"
     CDS             3223..3666
                     /locus_tag="VW077_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9455827.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            3782..4048
                     /locus_tag="VW077_001867"
     CDS             3782..4048
                     /locus_tag="VW077_001867"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9455828.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMRLVEGVVAYKKASGKPILD
                     TKREEVIFEKVGSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            4045..4419
                     /gene="crcB"
                     /locus_tag="VW077_001868"
     CDS             4045..4419
                     /gene="crcB"
                     /locus_tag="VW077_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9455829.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            4413..4742
                     /gene="crcB"
                     /locus_tag="VW077_001869"
     CDS             4413..4742
                     /gene="crcB"
                     /locus_tag="VW077_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9455830.1"
                     /translation="MVIVYLAIACGFGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            4861..5208
                     /gene="rplS"
                     /locus_tag="VW077_001870"
     CDS             4861..5208
                     /gene="rplS"
                     /locus_tag="VW077_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9455831.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            5250..5321
                     /locus_tag="VW077_001871"
     tRNA            5250..5321
                     /locus_tag="VW077_001871"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5283..5285,aa:Arg,seq:cct)
BASE COUNT         1626 a          998 c         1159 g         1736 t
ORIGIN      
        1 cagccaagat tttaccagct tgtccgatat ttctgcgact cattttgaac aacttcatat
       61 catgacaata gttcacagtg atatccaaag aaacaattct cccttgactt gtgacaatcg
      121 cttgagtctt catagcgtga aatttctttt taccagaata attcgctaat tcttttttta
      181 ggtcgattga tttttacttc cgtcgcatca atcattaccg tgtcctcaga actgagagga
      241 gttcttgaaa tcgtaacacc acctttgaac aagagttact tcaacccatt ggctccgacg
      301 gattaagttg ctttcgtgaa taccaagatc agccgcaatt tgttcataag ttcgatattc
      361 tcgcacatat tgaagagtgg ccataagaag gtcttctagg cttaatttag gttttcgtcc
      421 accttttgcg tgtttaagtt gataagctgt tttaatacag ctaacatctc ttcaaaagtc
      481 gtgcgttgaa caccaacaag acgcttaaat cgtgcatcgg ttagttgttt acttgcttca
      541 tcattcatag aactattata ccatattttg tttcgcagga agtctattga aaaatctcca
      601 gactggagaa ctcacggata gttcctaatc tggagatttc ttatttgcac ttttcttgta
      661 caactttagt ccatggtaaa taagcctcta aaacctcttt gtttacgaga gtttcctcgt
      721 ttggaagaca ttctagaaga taggatagat atttctcgct atttatacca gactaaaatc
      781 aaaaagcatt atataatagt gatatgaaat caactaaaga agaaatccaa gccatcaaaa
      841 cacttttaaa agactctcgt acagctaaat atcataaatg ccttcaaatc gttctatagt
      901 aaaacgaaac aagaacaggg caaatcgatc aggacagtca aatcgatttc taacaatgtt
      961 ttagaagtag aggtgtacta ttctagtttc aatctactat atttcgtctg atgggcaaat
     1021 cttataaaga gattatagag cttttagact gtaatcaaac aacgatttag cgaaatgtaa
     1081 aaaaatatga ggagttcgga ctcgactctc tccttcaaga aacacgtggt ggtcgtaacc
     1141 atgcatatat gacagttgag gaagagaaag cctttcttgc ccgtcatttg aaggctacag
     1201 aggcaggaga atttgttaca attgatgcct tatttcaggc ttataaaaag gagtgtggat
     1261 gaacgccttt ttagaagagc tttcacaagc ttatcctttt actcgttatg gacaatgcta
     1321 tatggcataa atcaagtacc ttaaagattc cgactaatat tggctttgca tttattcctc
     1381 catacacacc agagatgaac cccattgaac aagtgtggaa agagattcgt aaacgtggat
     1441 ttaagaataa agccttttaa actttggaag atgtcatgaa tcaactccaa gatgttatac
     1501 aaggactgga gaaggaggtg ataaagtcca tcgttaatcg gagatggact agaatgcttt
     1561 ttgaaagcag atgagtataa gtcctcgttt tgaaacctta gagcaggcca ttgcctccaa
     1621 agatttggaa aaagtccgag aagtttttaa gaaaatgaat tccacttgga ccatcaatga
     1681 aagtatggtt cgtgacaata gtacagctca ttatggacgt gttgaaacag ctatttcctt
     1741 cctgcctagt agcatggaaa ccgaacctac agatgagtct ggaacatgaa aacagcagtt
     1801 gatttcaaga aacaaaaaag aacagccgaa actgttctct tatcattatt tgagaccgta
     1861 ttttttgttg aaacgatcca cgcgtccatc tgcttgagtg aacttttgac gtccagtgta
     1921 gaatgggtgt gagtctgatg aaatttccac acggatcaat gggtaagttt cgccttcgaa
     1981 ctcaactgtt tcgttagagc gttttgttga accgctaagg aattggtaac cagtagttgt
     2041 gtccatgaag acaactgggc gatattctgg atggatatct tttttcattt tgcaatattt
     2101 cctttctgcc atggtctctt tgcgagccat agattgttac catactagtc tatcagattc
     2161 tgaaaagttt ggcaagtatt ttatcagttt ttaagcaagt tttttaactt ttggtagatg
     2221 atttcgtttt cttctaggct ataggaatta gcaccacttg ctagagggtg gcctccacca
     2281 tcatgctcct tggcgatttc attgatagga tggattttac tgcgtaagcg aacgcggtag
     2341 tggccatcag cctgttcgac aaaaattccc cagagactca ctctgtcaat gcgtccaggt
     2401 gcaccgacaa tggctgcagt ttcagcatcg gttacattgt attgtttcaa gacttcttga
     2461 ctcaggataa cgcgagcagc accattttca tccacttcca gatggtcgta gatgtagcct
     2521 tgcagtttag caattttgta gctcatagtg tccattttgc gagtgagagc cgcaaagtca
     2581 aagttatgtt ctctcaaata agcagccagg cgaagagtcc gtgcagtggt agaagggtag
     2641 aggaagcgac ctgtatcacc gacaattcct gcaaagagca actcagcatc gcgatctgcc
     2701 aaggctagtt gggttgtttg ggcaaatagg gtaatcatct cgctagcgct acttgaacta
     2761 gtatcgaccc aagacaggtc accgtataca tcatcatttg gatggtggtc aatcttaatg
     2821 agaaaatcac cttgactata gcgcttatca tcgatacgag cagtattagc tgtatcacag
     2881 acgatgacaa gtgcgccttg gtaggctcta tcttcaacaa gatccatctc agccatccaa
     2941 gtaagagttg gttcatcaaa accgacggct ttgatggttt tttctgggaa atgatgttcc
     3001 agcaaggctt tcaatcccac ctgacttccc aaggcatcag ggtctggttt catatgacga
     3061 tgaatgataa tggtgtcgta ttctttaatt ttttctaaaa tttgttggca aatctccata
     3121 aataacctca attctttctc atttcattgt agcacaagaa tttttatttt taaaatgaaa
     3181 aagatgtgat ataatggaga aagataaatt gaggaggacg atatggcatt agcaaaaatt
     3241 gtatttgcca gtatgaccgg taataccgaa gaaattgcag atattgtagc agacaaatta
     3301 cgtgacttgg gcttggatgt tgatgttgat gaatgtacaa ctgttgacgc ttcagacttc
     3361 ttggaagcag acatcgctat cgttgcgacc tatacttatg gtgatggtga attgccagat
     3421 gagatgatgg acttctacga agacctagca gatctcaact tgaatggcaa aatctatgga
     3481 gtggtcggct caggagatac cttctacgat gaattctgta aggctgttga tgattttgac
     3541 cgtgtctttg tgtcaacagg agcagaaaaa ggttcagagt gtgttaaagt tgatctttct
     3601 gccgaggaag aagatattga acgcttggaa caattcgcag aagaattggc tgctaaagta
     3661 ggataagcat aaaagtgcgc tgatgtaatc aatcagtgca tttttcttat cgagagttgg
     3721 gattttacta gcctaattga tggctttttg atacaataat tcaaataaag gaggagactg
     3781 tatggattta gatattattc gtcaagaaat cgatcaaatc gacgaccaaa ttgtcaaact
     3841 gttagaagaa cgaatgcgtt tagttgaggg ggtggttgct tataagaaag cttcaggtaa
     3901 accgatttta gatactaaga gagaagaagt tatttttgaa aaggttggga gtcgtgtaga
     3961 ggacaagcgc tatcaggaga ctattgtagc gactttttcg gacatactca aacgttcgcg
     4021 tgattatcag gatcaaaata tcaaatgaaa aaagaacaat tttatccgct aggaattttt
     4081 ctagctgcta tgttgggcgg acttgtccga tatctagttt ccacctggtt accagccagt
     4141 ccagactttc cttggggcac tctctttgtc aactatctgg gaattttctg cttgattttt
     4201 cttgtcaagg gctatctggt ctataagggg actagtaagg gcttgatttt agcactgggg
     4261 acgggttttt gcggaggttt aacaactttt tccagcctaa tgcttgatac tgtgaagctg
     4321 cttgatacag ggcgttattt tagtttagtc ctgtatttgc ttttgagcat cggtggaggc
     4381 ctactcttag cttattttct agggaggaag aaatggtaat tgtctatctt gcaattgctt
     4441 gtggtttcgg agcactcgta cggtatttct tttctcgcta taatcaagct tctaaattgc
     4501 cactcggaac gctcatagct aatcttttag gttgtttttt aattggagta ttctacaatc
     4561 atgtagagtc taaggaagta tatgctattc tagcaacagg attttgtggc ggtttaacaa
     4621 ctttttcgac cttgaatgac gaacttcaaa gactgctaag tgataagaag gtcttttatt
     4681 cttacctgac tttgacttac ataggtggtt tggttgcgat ttttttagga attttgctat
     4741 aaatttcttt acttttttgt gaaaatttgg taaactgtat cttgtgtgta atggacgcac
     4801 aagaatacag tttatccgct gaggaagttt cctcaagatt gacaaagata ggagaataaa
     4861 atgaatccat taatccaaag cttgactgaa ggtcaacttc gtacagatat cccatcattc
     4921 cgtcctggtg acactgttcg tgtacacgcg aaagttgtcg aaggtaaccg tgaacgtatc
     4981 cagatttttg aaggtgttgt tatcgcacgt aaaggtgctg gaatctcaga aaactacaca
     5041 gttcgtaaaa tctctaacgg tgtaggtgtt gagcgtatct tcccaatcca cactccacgt
     5101 gttgaaaaaa tcgaagttgt tcgttacggt aaagtacgtc gtgcgaaatt gtactacttg
     5161 cgtgctcttc aaggtaaggc agctcgtatc aaagaaatcc gtcgttaatt cgactaaaaa
     5221 actctgcttt tcagcagagt ttttatctag ctcccttagt tcaatggata taacaactcc
     5281 ctcctaagga gtagttgcag gttcgattcc tgcagggggc atgtaattag atatcaaaga
     5341 agtttgtcaa aaagtctttg atattaaaag aacgcataat ataggggatt gtctccataa
     5401 tattatgcgt tttattattg aaaaatttgg gcaatcatca gataaaattg agactatatc
     5461 gagtatataa atgtttgtat agtcaattta ttcaaaatcg tcaagttcta aattattta
//
LOCUS       DAVKGU010000105         5458 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKGU010000105 DAVKGU010000000
VERSION     DAVKGU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.106"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(11..385)
                     /locus_tag="VW077_001872"
                     /pseudo
     CDS             complement(11..385)
                     /locus_tag="VW077_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(765..1625)
                     /locus_tag="VW077_001873"
     CDS             complement(765..1625)
                     /locus_tag="VW077_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9455832.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(1641..2918)
                     /locus_tag="VW077_001874"
     CDS             complement(1641..2918)
                     /locus_tag="VW077_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9455833.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(2911..3462)
                     /locus_tag="VW077_001875"
     CDS             complement(2911..3462)
                     /locus_tag="VW077_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455834.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(3478..4737)
                     /locus_tag="VW077_001876"
     CDS             complement(3478..4737)
                     /locus_tag="VW077_001876"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9455835.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
BASE COUNT         1666 a         1234 c          893 g         1665 t
ORIGIN      
        1 gctagttgat ttttttagga cgatttattt ttacctctgt tgcatccaca atcaccgtat
       61 cctcagcact aagatgagtt tttgaaatcg taacaccact ttgaacaaga gttacttcaa
      121 cccattggct ccgacggagt aagttgcttt cgtgaatacc aagatcagcc gcaatttctt
      181 cataagtgcg gtattctcac acatattgaa gagtggccat aagaaggtct tctaggctta
      241 atttaggttt tcgtccacct tttgcgtgtt taagttgata agctgttttt aatacagcta
      301 acatctcttc aaaagtggta cgctgaacac caacaagacg cttaaatcgt gcatcagtta
      361 gttgtttact tgcttcatca ttcatagaac tactatacca tattttgttt cgcagggagt
      421 ctaatataaa aaaataaatt taagattggc aatacgaacg ttaaacagaa ttccatttct
      481 ctttttagaa tcaaaacgac aatattgtgg aatctctaca ggaacaatag actgcgacac
      541 taaaatatcc tcccttagtt ttactttact aatagtatat aggaggagag ccctgaatcc
      601 tagacccttt gttatgatgc gcttacaaac agagttggaa aaaatcttaa aagcagaaaa
      661 acgcatgata tcagccccta acacttgata caatgcgttt tattatgaaa aaatctactg
      721 gattttttat caaaataaac ttccagtagt cttttcactt tttattaata agttccttct
      781 tcaccttggc tagtcaagat aacaggtcca tctttcgtaa tgacaaattg gtgttcatac
      841 tgacatgaca atccaccgtc aatggtctta tgcgcccaac cagttttcat atctgtatca
      901 atttcccaat cgcctgtatt gatcattggt tcaatggtta agaccattcc ttcacgaaga
      961 cggagtccac gacctgcaat accatagtta ggaaccattg gttcttcgtg catagttggg
     1021 ccaacaccat gaccaaccaa atcacgcact acaccgtaac cacgactttc agcgtattct
     1081 tgaatagccg caccgatatc accgatacga tttccaacaa cagcttgctc aatacccttg
     1141 tacatagctt ctttggttac atccatcaag tttttgactt cttcggacgg tgtaccaaca
     1201 gcataagccc aacatgagtc tgctaaacca ccagaatagc tctgagtgta ttttttcatt
     1261 tgttcaacat tgttgaagtt taattttgag acatttaggt cagatttagc aatgggacct
     1321 cccaaaacca tatcaacttt gagcaaatca ccatctttca agatataatg acgagggaaa
     1381 gcgtgagcca cttcatcgtt aagagagcaa caggtagcat aaggatagtc catcatggca
     1441 ccgtcaaccc caatctgaag tggaaggaaa ttttcttctt tacaacgacg gcggacatat
     1501 tcttcaactt cccacatatc tacgcctggc ttaatcaaat cacgtaagcc tatatgaata
     1561 cttgctagaa aatcaccagc cttgtccata gcttcgattt cacgagctga ttttaatgtt
     1621 atcatctttt ctcctagttt ctaattaatt ttaacagtca catttgcttt tgaaacaatc
     1681 tggtgaccat gataaatatc gtaatcaatt atagctgacc gtctcgtatg atgaataatc
     1741 cgtgcctgaa tgcgcaatat atcatctatc tgaacggcct gcaaaaagta gatcagcatc
     1801 tgctcgataa tgagattgcg accactatta acaactaaat cttgggtcat gtgggtcaga
     1861 atttctgcca atacaccatt agccaaaact ccattttttt ctagcataaa gggttccact
     1921 gtaatgacta cttcatcatg gtgataagag agcttttgtc caatctgctc agaaaaagtt
     1981 ggtagagccg aaacttggga acggctcatc ttctccatga catctcgtcg cgtcacaacg
     2041 ccaagcaaag tttgattgct tcgaacaact ggtaccattt caaagtcttc tgcgatcatc
     2101 cgttgactca cattggcaat atttgtcgat aatccaacca aaaatagact acgagacata
     2161 accttatcaa ttgtcgtgct tggtgattta tcaccagcgt ctctcatggt tacaacgcca
     2221 acaacgacct gatgttgatt gataacaggg aaacggctgc tacgattctt acgaaccaag
     2281 tccaaataat ctttaactgt atctgtctct ctcagaaaac catactcatg actagggcga
     2341 taaagtttct caactgtcag aatatcagtc ttgatttgga catttgacaa ggctttattg
     2401 atcatggtag cgacggtaaa ggtatcatgc ttacttctta gaacaggaat ccctttttga
     2461 ttggccagtt taagcacatc atcatgaacc tgaaatcccc ctgtaaccag aactgcattt
     2521 tcattttcca aggctagcaa ctgaatacgg gttcggtctc cgacaatcaa gagcccccca
     2581 tcatgaaggt aagacaagat attttgttca gtcatggcac caattgaaaa cttactaaat
     2641 tctctctcta aaccttcttg cccagccaga acctcagaag aagtcacttc tgcaatttca
     2701 gcaaacgtta atctatctat agcaactttc tgggatttaa cacgaattgt tccacttcta
     2761 ggacgggtct ccacaattcc acggttttca gcttctttaa tagctcgata ggctgttcca
     2821 tcactaactc ctagatgatt cgaaatgcta cgaacactga cccttttacc tactggtaat
     2881 tcctccaaat agcttagaat ttcctgatgc ttactcattg aactctcctt ttacataccg
     2941 aaattcaaga taatcccgca tttttcttgt gtaacgatca cttcccttaa actgacggaa
     3001 caaactaaat ccagacttaa gagcaactct ttggctagct tcattttcaa ggtgggtaat
     3061 gataaataat tgttttaagc caaattcctc aaaagaaagc tgacaaattt ttctaacaac
     3121 ctctgtcata aatccttgcg accaagcatc ttttctcaaa aaatagccaa gctcagcttc
     3181 ttttttgatt tcatctaact tctcaaattt aatagaacca atcatttgtt gatttttctg
     3241 gtcacaaatt gcccacactc ccaaagggga cttcataaag taattggcca gtgcatattg
     3301 actttcttcc agacttgcct gcgttgggaa aataaattga agattttctg gatttgaagc
     3361 tatctcgcgg aagtcctgac tatcactaaa aaagaaagga cgcaaataca agcgatccgt
     3421 ttcaaaaaaa gaaaacattg ctaatttggt ccaaatattc ataaacacct ctacggttta
     3481 atcctcaaca agtctaatat ccgctcctaa attacgtaat ttttcgataa tatcagaata
     3541 accacgtaag ataaactcga tattggtaat ttcagtttta ccttcagcca taagcccagc
     3601 aatgactagt gcagccccag ctcttaagtc ggtcgcttta acactggccc cacgtaaatc
     3661 acgtccaccc gtgtacaaaa tatgaccatt tgttgtcgaa atatccgcat ccatctttgc
     3721 tagttcaaaa acatgattta cacgtttttc gtaaatcgta tcgacaattg taccacgacc
     3781 attcgctctt agtaaaagag gggtaagcgg ttgttgcaaa tcagttgcaa agcctgggta
     3841 aggagctgtc ttaatattga ttgctttcaa attagactgt tcctcgacaa aaatgctgtc
     3901 ttcagataca gtcattctca ctcccatttc ttccaactta gcaataaacc cttccaggtg
     3961 ttcgtaaaga acattattta tacgaattcc tttaccaact gcagcagcta aagatatata
     4021 tgttccagct tcaatgcggt ctggaatcac ctgatgacgt gtcccatgta atctttcaac
     4081 accatcaata atgatgatat tagttcctgc cccacggata tgggcaccca tattattcaa
     4141 gagagtagct acatcaataa tctcaggttc acgggctgca ttttcaataa tagtacgacc
     4201 atttgcttta accgcagcaa tcatcgtatt aatcgttgct cccacactaa ccgtatccat
     4261 gtaaatactt gcaccatgaa gtcctgtatc tttagcagat aacttcatgt tatctccctc
     4321 gtagctagca gtggcaccca tagcttcaaa cgccttaagg tgtaagtcaa tcggacgagg
     4381 accaagatca catcctcccg gtagaccaac tgtcgcttca ccaaaacggc ctaagaggct
     4441 cccataaaaa tagtaagatg cacgaagact gttaatttta ccataaggca ttggaatatt
     4501 ttgaacacct cttgggtcaa tctccaatac atcgtcataa cgcttaacag tagctcccat
     4561 caattccatg atttcgacaa gactggctac atccgaaata tctggaacgc aatccaaagt
     4621 caccacatca tcagccaaga taatagctgg aattaaggca acgacactat ttttagcacc
     4681 actaatagtg atttcacctt gcagtggtaa tccaccattg ataacaattt ttctcattct
     4741 aagttttcaa tactctttca agatttctaa taaggtcttc aaaataaagt atatcataaa
     4801 tttatccttt tttccatcct tttcaatttt attggataaa tcagagttga cgagttagta
     4861 actgaattac taatactcaa tgaaaatcaa agatcaaact aggaaactag ccgcaggttg
     4921 ctcaaaacac tgttttgagg ttgtagatgg aactgacgta gtcagtatcc tacatatacg
     4981 gcaaggcgat attgacgtga tttgaagaga ttttcgaaga gtataatctt tctcgaatcg
     5041 ctattgcccc ttttttatcc cctaaaactg agtaggatct aagactagat ctcttatcta
     5101 agtatcggaa tacggctgtt caactaaatt ttagcaagaa cttctcccct tctctcagtt
     5161 cacagaagcg ttttatacat accgtcctat cagacaatct attttaaaga taaccgtgac
     5221 tcaggatcac ttctatctgt cgttttctct ttatagtgat agacctcacg ggataagtcg
     5281 tacatctttc ctcgtgtata caagatataa ggacgttaaa aggttacagt gaaaatagaa
     5341 aatcttagta cgaattcaat tactctcgtg atttacgcat ataggttacg actacctttt
     5401 tggactttag agtttgagac ccccttatcc gctatatacg ccttactatc acgttcct
//
LOCUS       DAVKGU010000106         5045 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKGU010000106 DAVKGU010000000
VERSION     DAVKGU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.108"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            1..517
                     /locus_tag="VW077_001877"
                     /pseudo
     CDS             1..517
                     /locus_tag="VW077_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            520..1080
                     /locus_tag="VW077_001878"
     CDS             520..1080
                     /locus_tag="VW077_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455836.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEVI"
     gene            1080..1658
                     /locus_tag="VW077_001879"
     CDS             1080..1658
                     /locus_tag="VW077_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9455837.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            1651..4302
                     /locus_tag="VW077_001880"
     CDS             1651..4302
                     /locus_tag="VW077_001880"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9455838.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDARFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTINPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            4372..>5045
                     /locus_tag="VW077_001881"
     CDS             4372..>5045
                     /locus_tag="VW077_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEU9455839.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLID"
BASE COUNT         1516 a         1003 c         1138 g         1388 t
ORIGIN      
        1 ttttatttta ggatatcaag atcagaattt tggctataac attacgacct gtagttcctc
       61 gtatagtcta ggagattggg ttgtaattgg agtcgttgcg agagagaatg ccgcagagca
      121 gatcaaacag tatcaaaaat ttactgtgaa tatttctgat gaaacttcta tgcttgcgat
      181 ggagcaggct ggttttatca gtcatcagga gaaattggaa cgtttgggag tgcattatga
      241 aatttctgaa cgaactcaga ctcctatttt agacgcctgt ccacttgttt tagattgtcg
      301 ggtagatagg attgttgagg aagacggtat ttgccacatc tttgccaaga ttcttgagcg
      361 acttgttgcc ccagaactcc tggatgaaaa gggacatttt aaaaatcaac tgtttgcccc
      421 aacctatttc atgggagatg tatatcagcg cgtttatcgc tatctggaca agcgtgtaga
      481 tatgaagggc agtttcatca aaaaaagcga ggaagaagga tggcaagaac tgagctgcca
      541 gataaaatcg aaacagaacg tctcgtttta cgagtccgta ctgtggcgga tgctgaggat
      601 atctttgact atgctagttt gccagaggtc gcctatccag caggttttcc tccagtcaag
      661 accttggaag atgagattta ttatctggag cacattcttc cggagcgtaa tcaaaaggaa
      721 aatctcccag caggctacgg gattgtcgtc aaaggaacgg ataagatcgt tggctctgtc
      781 gatttcaacc atcgccatga agatgatgtg ctggaaatcg gctatacctt acacccagac
      841 tattgggggc gaggttatgt gccagaagct gcgcgtgcct tgattgactt agcctttaaa
      901 gatttgggtc ttcacaagat tgaactaact tgctttggat ataaccttca aagtaaacga
      961 gtcgcggaaa agcttggctt taccctcgaa gctcgcataa gagaccgaaa ggatgttcaa
     1021 ggaaaccgct gtgacagtct gatatatggc ttgctgaaga gcgagtggga ggtgatttga
     1081 tgcatctttt catcattggt gctccagcat caggaaaaat gaccattggt caagaattat
     1141 ctcgactgac ggatgctacc ctattttata accatcaagc cattgatttt gcactagaaa
     1201 tctatcagga ctatacagag gaaatgtggg aatttgttcg tggaatcact ttttcattcc
     1261 ttggagcaag tgcaagaaat cagcgatcag taattttaac agatgtaatt gatttttcaa
     1321 atcagtatca tctgatatat ttaaagcaaa ttcagaattt gttggatgat tatcatcaag
     1381 agattctttt tgttgagttg gaaacaagtc ttgaagagtg catacgtcga aatcgaacgg
     1441 agaatcgatt aaagcacaaa cccttaaaac gacatattga ggtatctgaa agagaaattt
     1501 tagagaccgc tgaaacactt caattaaatt cccaatatca accgaatgag ttgcaccact
     1561 actttaaaat aaataatacg aatttgtctg cagaagaagc tgctaagcag attcaaaata
     1621 aaataaacaa aatagagaaa ggacacacac atgtctaaag aactttcatc taaatacaat
     1681 ccagccgagg ttgaggctgg tcgttaccaa aaatggcttg atgctgatgt tttcaagcct
     1741 tcaggcgatc aaaaggctaa gccttattca atcgttattc caccaccaaa cgttacaggt
     1801 aaacttcacc ttggtcacgc ttgggataca actttgcaag atattatcat ccgtcaaaaa
     1861 cgcatgcaag gttttgatac cctttggctt cctgggatgg accacgcagg gattgccact
     1921 caggctaagg tagaggagcg cttgcgtggt gagggcattt cccgctatga ccttggtcgt
     1981 gagtctttct tgacgaaagt ctgggaatgg aaagacgaat atgccactac tatcaaggaa
     2041 caatggggca agatggggct ctctgtagac tactctcgtg agcgtttcac tcttgacgaa
     2101 ggtttgtcaa aagctgttcg taaggtcttt gtggaccttt acaagaaagg ctggatctac
     2161 cgtggtgagt ttatcatcaa ctgggaccca gcagctcgca cagccctttc tgatattgag
     2221 gtgattcaca aggatgtcga aggtgccttc taccacatga actacatgct ggaagatggt
     2281 tcacgcgccc ttgaagttgc tacaactcgt cctgagacta tgtttgggga cgttgcggtt
     2341 gcggttaatc cagaagaccc gcgctacaag gacttgattg gtaaaaacgt catccttcca
     2401 atcgctaata aactcatccc aatcgttgga gatgagcacg cagatcctga gtttggtact
     2461 ggtgtcgtga aaatcacacc tgcccacgat ccaaatgact tcttggttgg ccaacgtcat
     2521 aacttgccac aagtcaacgt catgaacgac gacggaacta tgaatgagct tgcctttgaa
     2581 ttttcaggca tggatcgttt tgaagctcgt aaggcagtcg ttgctaagtt ggaagaaatc
     2641 ggtgcccttg tcaaaatcga aaaacgtgtc cattcagtcg gtcactcaga acgtacaggt
     2701 gtcgtagttg agccacgctt gtctactcaa tggttcgtca agatggacca attggctaag
     2761 aacgccattg ccaaccaaga cacagaggac aaggtcgaat tctacccacc tcgtttcaac
     2821 gataccttcc ttcaatggat ggaaaatgtc cacgactggg ttatctctcg tcagctttgg
     2881 tggggtcacc aaatccctgc ttggtataat gaagcaggcg aaatctatgt cggcgaggaa
     2941 gccccagcgg gcgatggctg gaaacaggac gaagatgtcc ttgacacttg gttcagctct
     3001 gccctctggc ccttctcaac catgggctgg cctaataccg aaactactga ctttaaacgc
     3061 tactatccaa attcaacact cgtaacaggc tatgatatta ttcctttctg ggtatcacgc
     3121 atgattttcc aaggaattga atttacagga aagagcccat ttaaaaatgc tcttatccat
     3181 ggtctaattc gtgatgaaga aggacgtaag atgtctaaat ctcttggaaa tggcattgac
     3241 ccaatggatg ttattgataa atatggtaca gatagtcttc gttggttctt gtcaaacggc
     3301 tcagctcctg gtcaagacgc tcgtttctct tacgagaaaa tggatgcttc atggaacttt
     3361 attaacaaaa tttggaacat ttctcgctat atcctcatga acaatgaagg tttgacgctg
     3421 gatgtggcgc atgacaatgt cacaaaagtt gcaacaggtg aggctggtaa tgtgacggac
     3481 cgctggattc tccacaatct caacgaaacc attgcaaaag ttactgaaaa ctttgataag
     3541 ttcgagtttg gtgtggctgg tcatatcctt tacaacttca tctgggaaga atttgccaac
     3601 tggtatgttg agttgaccaa ggaagtcctt tatagcgata acgaagaaga gaaagtcatc
     3661 acacgttctg ttctactata cactttggac aagatccttc gtctccttca cccaatcatg
     3721 ccatttgtga cagaggaaat ctttggacaa atctcagaag gatctatcgt tacagcagaa
     3781 tacccaacta tcaacccagc ctttgaagac cttgctgctc acactggtgt ggaaagcctt
     3841 aaagacttga tccgtgccgt tcgtaatgcg cgtgcggaag taaacgttgc tccaagcaag
     3901 cctatcacta tccttgttaa gacaagcgat agcgacttgg aagccttctt taacagcaat
     3961 gtcaactaca tcaaacgttt cacaaatcct gaacacttgg aaatcgcatc aaccatccct
     4021 gcacctgaac tggctatgtc aagcgtcatc acaggagccg aaatcttctt gccactggta
     4081 gacctcttga atgtcgaaga agaattggca cgtctggaaa aagaacttgc caaatggcag
     4141 aaagaactcg acatggttgg caaaaaactc agcaacgaac gcttcgtcgc caacgctaaa
     4201 ccagaagttg tccaaaaaga acgcgacaag caagcagatt accaagctaa gtatgatgcg
     4261 accgtagcac gtattgatga gatgaagaag ttggtgaaat aaacacagaa acacggtgat
     4321 gagccgtgtt ttttggtata atgaaatcaa tatctattga tggagaaagt gatgtccaaa
     4381 caccctcatt atgaattgtt aaatttaatt ggctacggtc ttgccaagtt tgacaagctt
     4441 tttataaaag aatttcaatg ctctagcaag tcggagtttt atcgctatgt ggtttctctg
     4501 ggaattgctg aaacaactgg agttgtaaaa aatagaatgg atttatttga tccttatttt
     4561 gacaataatc gaaaaggttg gtggcagaaa gctgaagttt atcgttttcg taaagattta
     4621 attgatatga tgtttggaaa tgaagatgtt catagttatg ctgaaatagt taaaatgtta
     4681 cttgccagtg aagggaagaa aacaggcata accatcgttg aaaaaccaat agttcgaact
     4741 aaattcaaac gtctacagga aacgggaatg gaggctgaga attattttat ccttcatttt
     4801 gataaagaag aaaaattcca aggtggacag ttaaccgatg cccgtcttta tggtgatgga
     4861 tatgactttc aggtagatgt ccaagagtat tcttaccttg ccgaagtaaa aggtatccga
     4921 aaatctaagg ggcgtgtgcg tttaactgca aaagaatttg aaaaagtcaa ggagtttcaa
     4981 tcggatttta ttttatcctt ggtcacaaat ctagatgata ttccaaagtt agtgttaatc
     5041 gataa
//
LOCUS       DAVKGU010000107         5015 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKGU010000107 DAVKGU010000000
VERSION     DAVKGU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.109"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            1..345
                     /locus_tag="VW077_001882"
                     /pseudo
     CDS             1..345
                     /locus_tag="VW077_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(338..511)
                     /locus_tag="VW077_001883"
     CDS             complement(338..511)
                     /locus_tag="VW077_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU9455840.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(670..2277)
                     /locus_tag="VW077_001884"
     CDS             complement(670..2277)
                     /locus_tag="VW077_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9455841.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNEDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGAKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2430..3914
                     /locus_tag="VW077_001885"
     CDS             2430..3914
                     /locus_tag="VW077_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9455842.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQLAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            4009..4491
                     /locus_tag="VW077_001886"
     CDS             4009..4491
                     /locus_tag="VW077_001886"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9455843.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEYSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(4576..4892)
                     /locus_tag="VW077_001887"
                     /pseudo
     CDS             complement(4576..4892)
                     /locus_tag="VW077_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1443 a         1152 c          945 g         1475 t
ORIGIN      
        1 atttctttgg ttgcaggtct aacaaatggt gaattaatcg ctccaatgac ttacgaagag
       61 acgatgacga gcgacttttt tgaagcttag tttcagaagt ttctcttacc aacattaacc
      121 acaccatcgg ttattattat ggataatgca agattccata gaatggggaa gctagaactc
      181 ttgtgtgaag agtttgggca taaactttta cctcttcctc cctattcacc tgagtacaat
      241 cctattgaga aaacatgggt tcatatcaaa aagcacctca aaaaggtatt accaagttgc
      301 aatacctttt acgaggctct tttgtcttgt tcttgtttca attgactata ttatcagcgt
      361 aatagtctaa tagatagtta aatgtagttg gatttccact aaaggttgat gagtaagttt
      421 ttgtatctga acctgattgg ccgcaagcag ctaaaagcaa agcagatgca aaagtcaggc
      481 ctgcactaag gacacgcttc tttatgttca tcttctttct ccttaatagt gggaatttgt
      541 aaagttaatt gaatttcgag aatgaaagtt tataaacctt ggttataaaa aacaaaggat
      601 ttctgctttt tatacagtcc tcccttgttt ttatatgatt tcaattttaa atttttctgc
      661 aaaaaatatt tatagtaatt ccacacagaa agcatcccat ggaactaaga tttgtttttc
      721 aaagacttct tgagctaggg tgttttcaat caagacagat ttgacttttc cttctactgt
      781 caagtcttgc tcttcattgg acaagttagc cacaactagg aagcgacggt cgccatcctt
      841 acgtatataa gcaaagacct tatcagccgt atcaagcaat tcaaagtcag ctcgaattag
      901 ccagctattc tccttgcgaa tttggaccag tttctgatag gtatagaaaa tagaatctgg
      961 atttgccagc gcttcttgga cgttgatcat ctcgtaattt ggattaactg ccaaccaagg
     1021 ttgacctgtt gagaaaccag cgtttttgct ctcgtcccat tgcatagggg tacgggcatt
     1081 gtcacgtcca ataacacgga tactgtccat gatttcttca atcggaacac ctttttcaag
     1141 agcctcacgc gcatagttga gagattcaat atcttctact tgatccagtg tttcaaacgg
     1201 atagttggtc atcccaatct cctcaccttg gtagatataa ggagttcctc tcatgagatg
     1261 aagtaagatt gcaaaggctt tggcagattt ttcgcggtat tcttggtcat ttccccagat
     1321 tgagacaata cgagggaggt catggttgtt ccagaagagg gaattccagc cgtcctcaac
     1381 tcctaactct gtctgccatt tgttgaagat ttcttttaac ttagcgatat tcagctcttt
     1441 ttgatagtgc catttaggct gaccttcctg atactgaaga ccgatatgtt caaactggaa
     1501 gaccatagac aattcttgcc cctttggatc agagtagagc ttggcaatct ctggcgttgc
     1561 tccccaagtc tcccctactg tcaagagatc cttagctcca aaggtcgcct gattcatttc
     1621 cttgagatag ggatggagca taggaccatt attgactacc ttctcgtcag gaattttgcc
     1681 aatcatgtca ataacatcca tacggaaacc accaatacct ttatcaatcc agaagttcat
     1741 catctcataa attttctggc gaagtttttc attttcccag ttgagatccg gctgtttctt
     1801 gctgaaaaag tggagatagt attgaccaga tttttcatcg tattcccaag cagacccact
     1861 aaagatagaa tctaggtcat tgggttcatc gcgccagata tagtagtctc gctcagggct
     1921 attaggattt tcacaggcct ctacaaacca ggcatgctca tccgaggtat gattgaccac
     1981 caagtccatg atgatacgga tatcacgctt cttagcttct gcaatcagct gatccatgtc
     2041 ctccatggtt ccaaaaatag ccgcaatcgc ttgataatca gcaatatcat agccattatc
     2101 atccataggg ctgtcataaa cgggagaaag ccaaattgct gtgattccta gcttggctag
     2161 atagtccaac ttactggtaa ttcctggcaa atcgccaatt ccatcttcat tactatccat
     2221 aaaactcttt ggatagactt gatagactac ggcattatgc caccattttt cttgcatctg
     2281 tttacctcat tattttaata atctatagta tatgatagca ctttttaaaa ttttgtgcaa
     2341 gcgtttgctt aatcttttta atttcttttt tagctccgta gtttcctaac ttccaatttt
     2401 ttattataat agagtccaga ggtaaaaaaa tgaaaaaaca agcttttagt tctgaacaat
     2461 atttaaatct acaacgcgac cacatcttag agcgcattaa ccaatttgac ggcaagcttt
     2521 acctagagtt tggtggcaaa atgttagaag atttccacgc tgctcgtgtc cttcctggtt
     2581 atgaacctga caacaaaatc aagctcttgc aagaattgaa agagcaagtt gaggttgtga
     2641 ttgccattaa tgctagcaac attgaacatt ccaaagcacg tggcgactta ggcatttctt
     2701 atgaccaaga agttcttcgt ttgattgaca aattcaatga attagggatt tttgttggtt
     2761 ccgttgtcat tacacaatac gctggccaac tcgctgcaga tgccttccgc aaccaacttg
     2821 agaaaaacgg aattgattct tatcttcatt atccaatcaa aggatatccg acggatatgg
     2881 atcacatcat ttccccagaa ggcatgggca aaaacgacta catcaaaacc agtcgcaact
     2941 tgattgtcgt aaccgctcct ggacccggtt ctggaaaatt ggcaacgtgt atgtccaata
     3001 tgtaccacga ccaaatcaat ggcatcaagt ctggctacgc taaatttgaa accttccctg
     3061 tttggaatct tccccttcat catccagtta acttggctta tgaagctgcc acagctgacc
     3121 ttgatgatgt caacatgatt gaccccttcc atcttcaaac ctatggagaa accactgtca
     3181 actacaaccg tgatatcgaa atcttcccag tgctcaagcg catgttggaa cgtattctcg
     3241 gaaaatcacc atacgcttca ccgacagata tgggtgtcaa catggttggt ttcgctatta
     3301 cagatgacga ggctgctgtc gaagcttcta aacaagaaat catccgccgt tactatcaaa
     3361 cagttcttga cttcaaagct gaaaaagttg gcgaagctgc cgtcaagaaa attgagttgc
     3421 tcatgaacga cctcggtatc tcacctgcag accgtaaggt tgctgtcgtt gcgcgccaaa
     3481 aagcagaaga aactggtgga ccagccctag cctttgaatt gccaaatggg gaaatcgtca
     3541 ctggtaagaa ctcagaactc tttgggccta cagccgctgc cttgatcaac gccatcaaaa
     3601 aatcagctga catcgctaaa gaagtaaaac taatcgagcc tgaagttgtt aagccaatcc
     3661 aaggtcttaa aatcgatcat ctcggtagcc gcaatccacg ccttcattca aatgaaatcc
     3721 tgattgcact tgctatcaca gctacagaaa atcctgatgc tgcccgcgct atggaagaac
     3781 tcggcaacct caaaggaagc gaagcccact caaccatcat cttgactgat gaagacaaga
     3841 atgtccttcg taaactgggt atcaacgtaa cctttgaccc atactaccaa tacgaccgct
     3901 tatatcgtaa gtaaagattt caacctctcc agcctcggag aggttttctc tctgtctcag
     3961 gagcaacctt tatgatataa tgagagaaga aaactgaaag gatttaccat gtcaaaagaa
     4021 gttattgtcg aaagttttga acttgaccac accattgtca aagcacccta tgttcgcttg
     4081 attggggaag aaacaggacc aaaaggagac atcatctcca attatgatat tcgcttggtg
     4141 caaccaaacg aatactctat ccctactgcc ggccttcaca ctatcgagca cctcttagcc
     4201 aaactcatcc gtacccgcat tgacggcatg attgactgtt caccatttgg ttgccgcaca
     4261 ggcttccaca tgattatgtg gggacgtcac accagtgcta aaatcgcagc tgttatcaag
     4321 gattcgctca aggaaatcgc tgaaactact acttgggaag atgtcccagg gacaaccatc
     4381 gaatcttgcg gaaactacaa ggaccacagc ctcttctctg ctaaagaatg ggcaaaactc
     4441 atcttggaac aagggatttc agatgatgcc tttgaacgtc atgtgattta aaatcgagta
     4501 ggggataatc tctagcccct ctcacaccac cgtacgtgcc gttcggcata cggcggttca
     4561 actaactttt aacgcctatc gttcaaggta ataatccaaa cacaaagcca gtccactttc
     4621 tcaaggactg gttttgatat agcacgttta agtaccgact tctgagctac taattgataa
     4681 tggtcgcccc agccagatac cttatctgct atccatttag gaactcctaa cttaagcaat
     4741 ccccataatc gtctcgattt cttcttccat tgcttccaga taatcactcg taggcgagta
     4801 cgcaagcgct catctatgct ggcgactata cttttcatat ttcccaatga gaaatagttt
     4861 atccatcctc gaatgaacaa attcagttgc tctcgtgatt tacgcatata ggttacgact
     4921 acctttttga actttagagt ttgagacccc cttatccgct atatacgcct tactatcacg
     4981 ttcctgttcg taaagccacg atttcgctat ccctt
//
LOCUS       DAVKGU010000108         4985 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKGU010000108 DAVKGU010000000
VERSION     DAVKGU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.110"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            268..582
                     /locus_tag="VW077_001888"
     CDS             268..582
                     /locus_tag="VW077_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU9455844.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            598..984
                     /locus_tag="VW077_001889"
     CDS             598..984
                     /locus_tag="VW077_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU9455845.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1013..2398
                     /locus_tag="VW077_001890"
     CDS             1013..2398
                     /locus_tag="VW077_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9455846.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2401..2553
                     /locus_tag="VW077_001891"
     CDS             2401..2553
                     /locus_tag="VW077_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU9455847.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2576..3826
                     /locus_tag="VW077_001892"
     CDS             2576..3826
                     /locus_tag="VW077_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEU9455848.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKDICQPYQLTDIQKKI"
     gene            3784..3993
                     /locus_tag="VW077_001893"
     CDS             3784..3993
                     /locus_tag="VW077_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEU9455849.1"
                     /translation="MSTIPVDRYPKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEV
                     MEIRLRSKLKSVLIVHYLKILYNRN"
     gene            4042..4125
                     /locus_tag="VW077_001894"
     CDS             4042..4125
                     /locus_tag="VW077_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEU9455850.1"
                     /translation="MLVFQMHNVDKTSTVLKQTKNSDYADK"
     gene            4250..>4985
                     /gene="erm(B)"
                     /locus_tag="VW077_001895"
     CDS             4250..>4985
                     /gene="erm(B)"
                     /locus_tag="VW077_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEU9455851.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT         1704 a          883 c         1016 g         1382 t
ORIGIN      
        1 actcaattta ctactaattt actacttatg aatgagcttt gatacgacga tttatccttg
       61 aaaagtgaag atataaagat acttccaata aaatttgaat atttaatagg tagacacttc
      121 aaaaaatgag gtgtctattt ttttacccga ttttgaaagg aagtgaactt atgaaaacaa
      181 aaaatcaaga atcaaaaggt cgttccccac tctttaagac catcaaacat tcattcagcc
      241 aataaaaaag aaaggatagg taaaaatatg gaacttaaat ttgtgattcc caacatggaa
      301 aaaacattcg gcaatttaga atttgctggc gaggataaag tcgttcagcg aagaatcaac
      361 ggacggctaa ctgtcttatc aagaagctat aatctctatt ctgatgttca aagagcagat
      421 gatattgtgg tggtgcttcc tgctgaagct ggcgaaaaac atttcggctt tgaggaacgt
      481 gtgaagttag tcaatccacg tattaccgca gagggctaca aaatcggcac tcgtggtttt
      541 acaaattacc ttttacatgc tgacgacatg ataaaagaat aaagaaagag aggaaaaatg
      601 atgagattag caaatggcat tgtattagat aaagacacga cttttggaga attgaaattc
      661 tctgctctac gtcgtgaagt gagaatccaa aatgaagacg ggtcggtttc agatgaaatc
      721 aaggaacgta cctatgactt aaaatccaaa ggacaaggac gcatgattca agtaagtatt
      781 cctgccagcg tgcctttgaa agagtttgat tataacgcac gggtggaact tatcaatccc
      841 attgcggaca ccgttgctac tgccacctat caaggagcag atgttgactg gtatatcaag
      901 gcagacgata ttgtgctgac aaaggattct agttcattca aagctcaacc acaagcaaag
      961 aaagaaccga cacaagacaa atagtcgcta ggtagaaagg agactttttc gcatgaaaca
     1021 gcgtggtaaa aggattcgcc catctggtaa agatttagtc tttcatttta cgatagcgtc
     1081 actcctgcct gttttcctgc tggttgtcgg actgtttcat gtgaagacaa tccagcagat
     1141 caactggcag gattttaacc tatcacaagc agataagatt gacattccct atttaattat
     1201 cagtttcagt gtcgcaattc ttatctgctt gctggtagcg tttgtattca aacgggttcg
     1261 ctatgatacg gttaaacaac tttaccaccg tcaaaaactg gcaaagatga tacttgaaaa
     1321 caagtggtat gaatctgaac aggtcaaaac agagggtttc tttaaagata gtgctggtcg
     1381 tacaaaggaa aagataacct acttccctaa aatgtattat cgacttaaaa atggcttgat
     1441 acagatacgg gtggaaatca cgctgggaaa atatcaagac caactcttac acttggaaaa
     1501 gaaattagag agtggcttgt actgtgagct gacggataaa gagttaaagg attcctatgt
     1561 ggaatatact ttgctctatg acaccatagc cagtcgtatt tctattgatg aagtagaagc
     1621 taaagatggt aaacttcgct taatgaaaaa cgtatggtgg gaatatgata agctccctca
     1681 tatgttgatt gctggtggta caggtggcgg taaaacttac tttatactga cactgattga
     1741 agccttgctt catacagatt caaaactgta tattcttgac ccgaaaaatg ctgaccttgc
     1801 ggacttaggt tctgtgatgg caaatgtcta ctatagaaaa gaagacttgc tttcttgcat
     1861 tgaaacattc tatgaagaaa tgatgaaacg tagtgaggaa atgaagcaga tgaagaacta
     1921 taagactggc aaaaattatg cttacttagg tctcccggca cacttcttaa tctttgatga
     1981 atacgtcgct ttcatggaaa tgctgggaac aaaagaaaac accgcagtta tgaataagct
     2041 gaaacagatt gtcatgttag gtcgtcaagc tggcttcttt ctaatactgg cttgtcaacg
     2101 tccagacgca aaatatttag gcgacggaat ccgtgatcag tttaatttca gagtggcttt
     2161 aggtcgtatg tctgaaatgg gctatggcat gatgtttggc agtgacgtac aaaaggattt
     2221 cttcttaaag cgaatcaaag gtcgtggcta tgttgatgta ggaacaagtg tcatatcaga
     2281 gttttatact ccccttgtac caaaaggata tgatttcttg gaggaaatta aaaagttatc
     2341 caacagcaga cagtccacgc aggcgacgtg cgaagcggaa gtcgcaggtg tggactgatc
     2401 ttgctggctg gtgtggcaat agccacgcca gcacttaacc ccccgtatct aacagggggg
     2461 tacaaatcga caggaaacag tcaaaaaaac attagaaaat cctttggtta caagggattt
     2521 acaaaatttc agcgtatgtc aaatgggctt taaaagttga catacgcctt tttgattgga
     2581 gggattttta ctgaatgaac aaacttggtt acagcattta aaagaaaaac gcttggctta
     2641 tggactatct caaaaccgtt tagctgttgc gactggtatt acaaggcagt atctaagcga
     2701 tattgaaaca ggaaaagtca agccatcaga ggatttacag cagtcccttt gggaagctct
     2761 ggaacgcttc aatcccgacg ctccccttga aatgctgttt gattatgtaa ggattcgctt
     2821 tccgacaaca gacgtacagc aggtggtcga aaacatctta caactgaaac tgtcctattt
     2881 tcttcatgag gactatggtt tctattctta ttcagagcat tatgctttag gcgacatatt
     2941 cgtcctttgc tcccatgaac tggacaaagg agttctggtg gaattgaaag gtcgtgggtg
     3001 cagacaattt gaaagctatc ttctggcaca acaaagaagc tggtatgagt tctttatgga
     3061 cgttttggtg gctggcggtg tgatgaaacg ccttgacctt gccattaacg ataagacagg
     3121 gattttaaat atccctgtac tcactgaaaa gtgccaacag gaagaatgta tctccgtctt
     3181 ccgcagtttt aaaagctatc gcagtggcga actggtacgc aaagaggaaa aggaatgtat
     3241 gggaaacacc ctctatatcg gttcattaca aagtgaagtt tatttctgta tctatgaaaa
     3301 ggactacgag cagtacaaga aaaatgatat tcccattgaa gacgcagaag taaaaaaccg
     3361 ttttgagatt cgattgaaaa atgagcgtgc ctattatgca gtccgtgatt tactcgtcta
     3421 tgacaatcca gagcataccg cctttaaaat tatcaatcgg tatatccgtt ttgtagataa
     3481 agacgattcc aaacctcgtt ctgattggaa actgaatgaa gaatgggctt ggtttattgg
     3541 gaacaatcgt gaacgattaa aactaaccac aaaaccagag ccttactcct tccaaaggac
     3601 gctgaactgg ctatctcatc aagttgcccc gaccttaaag gttgcgatta aacttgatga
     3661 aatcaaccag acgcaggttg taaaagacat tctcgaccat gcgaaactga cagaccgaca
     3721 caagcagatt ttgaagcaac agtcagtaaa agaacaggac gtgataacaa caaaaaaaga
     3781 tatctgtcaa ccataccagt tgacagatat ccaaaaaaag atataatggg agataagacg
     3841 gttcgtgttc gtgctgactt gcaccatatc ataaaaatcg aaacagcaaa gaatggcgga
     3901 aacgtaaaag aagttatgga aataagactt agaagcaaac ttaagagtgt gttgatagtg
     3961 cattatctta aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta
     4021 attaagaagg agggattcgt catgttggta ttccaaatgc ataatgtaga taaaacatct
     4081 actgttttga aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat
     4141 tcctaccagt gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg
     4201 tttaacttct gtatttgttt atagatgtaa tcacttcagg agagattaca tgaacaaaaa
     4261 tataaaatat tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca
     4321 attgaattta aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac
     4381 gacgaaactg gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt
     4441 caacttatcg tcagaaaaat taaaactgaa tactcgtgtc actttaattc accaagatat
     4501 tctacagttt caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca
     4561 tttaagcaca caaattatta aaaaagtggt ttttgaaagc cgtgcgtctg acatctatct
     4621 gattgttgaa gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct
     4681 cttgcacact caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc
     4741 taaaccaaaa gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga
     4801 taaatattgg aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca
     4861 actgtttact aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag
     4921 taccattact tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag
     4981 gaaat
//
LOCUS       DAVKGU010000109         4944 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKGU010000109 DAVKGU010000000
VERSION     DAVKGU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.111"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            122..718
                     /locus_tag="VW077_001896"
                     /pseudo
     CDS             122..718
                     /locus_tag="VW077_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            794..1243
                     /locus_tag="VW077_001897"
     CDS             794..1243
                     /locus_tag="VW077_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9455852.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            1256..1915
                     /locus_tag="VW077_001898"
     CDS             1256..1915
                     /locus_tag="VW077_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455853.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKGSLFTLLGPSGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMNEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     K"
     gene            2042..2347
                     /locus_tag="VW077_001899"
                     /pseudo
     CDS             2042..2347
                     /locus_tag="VW077_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            2337..3989
                     /locus_tag="VW077_001900"
                     /pseudo
     CDS             2337..3989
                     /locus_tag="VW077_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4092..4784)
                     /locus_tag="VW077_001901"
     CDS             complement(4092..4784)
                     /locus_tag="VW077_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9455854.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1545 a         1052 c          854 g         1493 t
ORIGIN      
        1 atattttagc agtcaaagtg tactgttata gattcaatac actataggat agaattgtag
       61 gttttaaaaa cgcttatatt gttttaaaat aaacttaaat aacacaacgg aggtatccat
      121 catgaaaaag aaatggatgt attatgctgc ttgttcttct aatgaatctg ccgatgacag
      181 ttcatctgat aaaggagacg gtggttcgct agtcgtttat tcaccaaact cagaaggctt
      241 aattggagca actattcctg cctttgaaga aaaatatggt atcaaaatag aactgattca
      301 agctggtact ggagaacttt tcaaaaaact agagtcagaa aaagaagttc ctgtagctga
      361 tgttatcttt ggtggttctt atacacaata tgctacccac ggagaactct ttgaaaacta
      421 tatttcaaaa gaaaatgata atgttatcaa agaatatcaa aacacaactg gcttctctac
      481 tccttataca ctagatggta gtgttttaat cgtccaccct gatttaacta aaggcatgaa
      541 catcgaagga tatagcgacc ttctcaaacc tgaactaaaa ggaaaaatcg caactgctga
      601 cccagcaaac tcttctagcg cctttgctca attaacaaat atgctacaag ctcaaggtgg
      661 ttacaaagat gataaggctt ggtcttatgt aaaagatctt ttcacactta ttgatggtat
      721 agtaaaatga aataagaaca agacaaatta atcaggacag tcaaatcgat ttctaacaat
      781 gttttagaag tagatgtgta ctattctagt ttcaatatac tataaaatcg gtttaagttc
      841 atctggtgtc tataaagcag tcgctgatgg agaaatgact gttggtctct cttatgaaga
      901 tccagcagtt aaactcttaa atgacggagc taacattaag gtagtctatc caaaagaagg
      961 aaccgtcttc ctacctgcta gtgctgctat cgttaaaaaa tctaaaaata tggaaaatgc
     1021 caagaaattt atcgatttta ttatctctca agaagtacaa gatacacttg gtacaaccac
     1081 tactaaccgt cctgttcgta aaaatgctaa aacaagcgaa aacatgaaac caattgacaa
     1141 aatcaaaaca ctcactgaag attatgatta tgtcatcaag aataaatcag atatcgttaa
     1201 gaaatacaac gaagtcttta cagatatcca atctaaacag taaaagaggt tcactatgag
     1261 tgagatcaaa attattaacg ccaaaaaaat ctaccacgat gtccctgtta ttgagaattt
     1321 gaacattaca attccaaaag gaagtctctt tacccttctt ggaccttcag gatgtgggaa
     1381 aacgaccctt cttcgtatga ttgcaggttt caacagtatc gaaggtggag aattttactt
     1441 cgatgataca aaaatcaata atatggaacc cagcaaacgc aatatcggga tggttttcca
     1501 aaactacgct attttcccac atttgactgt ccgagacaac gttgcttttg gtcttatgca
     1561 aaagaaggtt ccaaaagaag aattgattca acagaccaac aagtatcttg aactcatgca
     1621 aattgctcaa tatgcggatc gaaagcccga taaactcagt ggtggacaac aacaacgtgt
     1681 caccttggca tgcgccttag cggttaatcc aagtgttctc ctcatgaacg agccacttag
     1741 taatctggag gccaaacttc gcttggatat gcgtcaagcc atccgagaaa tccaacacga
     1801 agtgggaatt acaactgttt atgtaaccca cgaccaagaa gaagccatgg ctatttcaga
     1861 ccaaattgct gttatgaaag atggggtgat ccaacaaatc ggccgaccaa aataactcta
     1921 tcataaacca gctaatgagt ttgtggcaac ctttatcgga cgcacaaata ttatccctgc
     1981 caatcttgaa aaacggagcg acggcgctta tatcgtcttt tcagatggct atgcccttcg
     2041 aatgccagct cttgatcagg ttgaggagca agctattcat gtaagcattc gtcccgaaga
     2101 gtttatcaaa gatgaatctg gagatatcga aggaactatt agcgatagcg tctatcttgg
     2161 actaaatacg gagtatttca ttgagacagg ttttgcctca aaaatttaag ttagtgaaga
     2221 atcaactttt gaagaagaac tacaaaaagg caatcgtatt cgtctacgaa tcaatacgca
     2281 aaaattaaac atcttttctg tagatggttc ccaaaacctg ataaaaggag tcaaccatgg
     2341 aatgtaaaaa actaaatatt tggacagcct cctctttctt catctttctt acctatcttg
     2401 tctttctcgt ttatcctatc gttaccgtgc tcaagcaagc acttatacat gaaggacaat
     2461 tctcactatc taattttgtc actttcttta gtaaagccta ctactctgag acactagtca
     2521 acagtttcag ggtttccatt accgctactg tcacttcctt agttgtagga accctattag
     2581 cttatctctt ctctatgtat gacttcaagg ggaagaaatt tctacaaata ttgattatca
     2641 ttgcttccat gtcagctcct ttcgtaggag cctactcctg ggttctcttg ctgggacgaa
     2701 atgggggcat cactaaattt ttgacaaatg cccttcatct tccagctatc gatatttatg
     2761 gattcaaagg aattgtactt gtctttacac tgcaactatt cccactggta tttctatatg
     2821 ttgctggaac aatgaacagt attgacaatt ctctacttga agcagctgaa agcatggggt
     2881 ccttcggatt taaacctatc gtaacggttg ttttacctct tctagttcca accttactag
     2941 cagctccttg cttgtattta tgagagcatt ctcagacttt ggaacgccta tgttgattgg
     3001 cgaaggatat cggactttcc ctgtcctgat ttatacccaa tttattagcg aggttggagg
     3061 aaattctgct tttgcaatta tggcgattat cattgccttg gcaattttcc ttatccaaaa
     3121 acacattgca aaccgctaca gtttcagcat gaatccgctc catccaattg agcctaaaaa
     3181 aactacaaaa ggaaaaatgg ctgccattta tgcaacagtc tacggaatta tctttatctc
     3241 tgttttacct caaatctact taatttatac ctctttccta aaaacatcag gtatggtatt
     3301 tgttaaaggt tattctccaa acagttacaa ggtagctttc aatcgtatgg gatctgctat
     3361 tttcaatacc attcgtatcc ctttgattgc cttagttcta gttgttctat ttgcgacatt
     3421 tatctcctac ctagccgtta gaaaacggaa tttgtttaca aacttaattg acagcctcag
     3481 tatggtacct tatattgtat caggaaccgt tctagggatt gccttcattt cttccttcaa
     3541 tactggtcta tttggaagtg gatttcttat gattacaggg actgctttca tcttgattat
     3601 gtctctatct gtcagaagat taccttatac tattcgctca tctgttgcta gcttacaaca
     3661 aatagcacca agtattgaag aagctgctga aagcttagga agtagtcgtc tcaatacctt
     3721 tgctaagatt acaactccaa tgatgctatc gggtattatt tctggagcca tcctatcttg
     3781 ggtcacactg atttcagaac tctctacttc tatcctctat cctcctctac aatgtcaaaa
     3841 caagaacaat gactgtagct atttatacag aggttctcag aggaaattac ggtgtagccg
     3901 cagccttgtc aactatcctg actgttctaa cagtaggttc cttgctcttg tttatgaaaa
     3961 tctctaaaag caatagcatt acactttagt tttccacatc aaaaacagcc gaaaggagta
     4021 ccctcggctg ttttttctta tcttgatatt cttatcaaat ccctagctca tggcaagcaa
     4081 gtctagactg attaaataga gccttcttcc atcttctcac gtcctagctc tcggtaacta
     4141 cggtcaccga caacttcaac cctaaactga ccgtcctcat attcaagaat cgtcacgcta
     4201 ccattatcta gaccatgcgg atgcatgcca ttaatcagat aaacaatggt tccaatagtc
     4261 attccatggc tgacaacaag ggcgttacct ccaccttgat cttccatttc ttttgcaatc
     4321 atttcaaagc cttccttaat tcggccactg agtttttccc agccttcagc ccaaccagct
     4381 gtatcgacct ctaccaagcc ctcagccagt tcagcataag acaattggtg aacgtggtcc
     4441 acattaaaga tacgaggaat aatgcccatg aaaagatcgc catcataggc tccatcaaaa
     4501 ctaccgaaac accattctct gatacgcttg tccatgcgat aagggatttt cccctgcaag
     4561 ccaagctctt caaggataat tcccatggtc tgaatggtac gaccagaatc actcgaataa
     4621 gcgcgctcaa actgtagatc agattctcgc aaaccaattc ctaactcttg aatccctcgt
     4681 tcaccttcag cagttaaggg agtatcgctc caaccttgcg cgcgaccaat cgtgttaaac
     4741 atggtcttgc catgacgtac caaatacaat cgtactttta ccattttccg tcctccgttt
     4801 gcttctatta tatcatgaat gataaaataa aagccaccca tacaggcgac ttttgaagga
     4861 gattattatg aaaaagttta ggagttcatt aaataaagat attagatgaa aatcaaattc
     4921 aaactaattc agtgttatct gttt
//
LOCUS       DAVKGU010000110         4767 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKGU010000110 DAVKGU010000000
VERSION     DAVKGU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.112"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..494
                     /locus_tag="VW077_001902"
     CDS             <1..494
                     /locus_tag="VW077_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455855.1"
                     /translation="NSIVEGLLRKEFTSKKLYSPVVTSLTLKAMPFIKYLLTDLVYFD
                     NGYIIPFGFRYTDMVKLKTDTIHCSIFDNLFGTLPFKFEPLEQYQIIYDAQNSEIVVN
                     VLNLEVTQGDIVCSVLKEYIKEHYKSSIKVSLRNCSKFFRDNLTGKMRGIINKENINN
                     EIW"
     gene            510..2015
                     /locus_tag="VW077_001903"
     CDS             510..2015
                     /locus_tag="VW077_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU9455856.1"
                     /translation="MDNENEISKFFLSSENVKDMKTIANDILNMGLDFKLSEKVSQKK
                     MKEHDDLNFLSLEESIDNLPQLLEEFREKVIPYCTNFSSPNFMGFPDSGNSLGGLSGS
                     LLSDLLQQNLINASFCAPVATIMEINVIQWLRKVLGYSTSDVHNIMEVGGIVTYGGTG
                     SNSTAMLLARENKDGNTLELGVRNPRNYKVIVPKGIGHYSIASSLKWLGLGNNIIEVP
                     TENYRYNLKKLREALINHSNEIMAVVAYAGDSRTMTIDNLSEVADLVKTLTPNVWLHC
                     DACHGFSLAFSKKYKDRIKGIEKFDSISTDPHKVLGIPYCISVLLVKTPQSLNLIHSQ
                     SDLIMNENLALGQTTPFLGSKSWISLKLWLVAKHLGSEKIGAMVDKRIELTNYLQDLI
                     LKSSKLILLNRTDINSVMFMYIGDKHQEVSLSSVQNINNINKSIYDRLKDEGIYYLHQ
                     FPIIDNTETIYRGFELCPLRFMSGNPNLTIEELQKMVIEVENLGDRFVGES"
     gene            2019..3491
                     /locus_tag="VW077_001904"
     CDS             2019..3491
                     /locus_tag="VW077_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HEU9455857.1"
                     /translation="MSKELYNKFILTKEAGNIIYDGIEFGRDEIDRRILEISKILTAD
                     IVNLSQEAVAISCNKKINFFFLFLVVNCCDGVVMPVYARTPENKLQHICERYDINYMV
                     SDHEINLVGYSLIYHGSVFSEEYFIYECNKSFPKHEELKGIELLMLSSGTTGVSKAIS
                     LSLDNILSNTRSIQKYIIPTREDRCLIIKEITHSSSLISELINSLLSKNSVLLSDLIP
                     TVSSLGYLISTYGVTILYAIPDFLLFMNSIKFINNYRQLLGKLRLVQVVGAFCPPNYI
                     TDFHKNWPKIDLFNGYGLTEASPRVAVLGGEELYANPRCVGYPISGVGVHIDTSSNNT
                     QEGEICVTGPNIMVEYYLNEELTSKIKIGGMLRTGDIGYFDDKNRLYILGRKDNRYIK
                     SGKNIFPEEIESVVRKLPFISEVIVYPKIESGRLISILEYTICNDEEKDLNQEASEII
                     KLCKDNLEDYKIPDQYRKVKNIPHTSTGKVKRDRNNYYNE"
     gene            3484..>4767
                     /locus_tag="VW077_001905"
     CDS             3484..>4767
                     /locus_tag="VW077_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9455858.1"
                     /translation="MNELCKKYVYQLQNELNQYCDNLEGIHVIAYGSNGSNDLSISSD
                     FDVCLIVNDDSFVAMNQRLICDFTKLFHFQENLPIDKDIPLSNKLIYLSSEVELFLDN
                     PPFISDSKKVKIEDIKKTKNFLTSRTMKQRLLLNILTTDSTVLFGDEDYVNNLKYRAW
                     EDIEKIIERAYNIKLTTKNENEFLNLLYQNPVSKASGEYYLGYKKEKRNVVKHLERNV
                     HRLAILNEKKVEDIIDLSENVNRLLLKDYSFLFPSETIKKMISTYPEKKSCSIKEVLS
                     SKLTIASNNFLITNGSLEGMNIIIANLVQNNIGYLTPTFWGYGDSAKRYNKSVISAPL
                     SDIYNYNVTEIDKISKSCELMIICNPNNPTTATLTKNQIENLLLNNPQTYFLIDESML
                     PYKYDFFNYTMIPFVEKYTNLIVCSSISKSLCAPGL"
BASE COUNT         1773 a          585 c          841 g         1568 t
ORIGIN      
        1 ataattctat agttgaaggt ctgctgagaa aagaatttac ttctaaaaaa ttatatagtc
       61 cagttgttac ttcactaact ctaaaggcaa tgccatttat aaaatattta cttactgatt
      121 tggtttactt tgataatggg tatattatcc cgtttggctt tagatacaca gacatggtaa
      181 aactaaaaac agataccatc cattgttcaa tatttgataa tttatttgga acattaccat
      241 ttaaatttga acctttagaa cagtatcaga ttatctacga tgctcaaaat tcagagattg
      301 tggttaatgt tctaaattta gaagtgacac aaggtgatat tgtttgctcg gttttaaaag
      361 aatatatcaa agaacactac aaaagttcaa taaaagtttc tcttcgaaat tgttcaaaat
      421 tttttagaga taatctgaca ggaaaaatgc gtgggataat aaataaagag aatataaata
      481 atgaaatttg gtaggaggca aactcataca tggataatga aaacgaaatt tcaaagtttt
      541 ttctgagttc agaaaatgta aaagatatga aaactatcgc aaatgacatt ttaaatatgg
      601 ggttggattt taaattaagc gaaaaagttt ctcagaaaaa aatgaaagaa cacgatgatt
      661 taaattttct cagcttagaa gagagtatag acaatcttcc gcaacttttg gaagaattca
      721 gggaaaaagt aattccttac tgtacaaatt tttcttcccc taattttatg ggttttcctg
      781 actcaggaaa ttcattaggc ggtctttcag gtagtttgct tagtgattta ttacagcaaa
      841 atttgattaa tgcatctttt tgcgctcccg ttgctactat tatggaaata aatgtaatcc
      901 agtggttacg aaaagtttta ggatattcca cttctgatgt tcataatatt atggaagtag
      961 gcggcatcgt tacctatggt ggtacaggaa gtaattctac agctatgctt ttagcaagag
     1021 aaaataaaga tggaaatact cttgaattag gcgtaagaaa ccccagaaac tataaggtaa
     1081 ttgtgccaaa aggaattggt cattatagta tcgcatcaag tttaaagtgg ctgggattgg
     1141 ggaataatat tattgaagtt cctacagaaa attatcgcta taacttgaag aaacttaggg
     1201 aagcactaat caaccattct aatgaaataa tggcagtggt tgcatatgct ggagatagta
     1261 gaacaatgac tattgataat ctatctgaag tagcagattt ggtaaagaca cttaccccaa
     1321 atgtttggtt acactgtgat gcttgtcacg gattttcatt agctttttct aaaaagtata
     1381 aagataggat aaaaggaatt gaaaaatttg atagtatttc gacagatccg cataaagttt
     1441 taggaattcc ttattgcata agtgttttgt tagtgaaaac acctcaatcg cttaatctaa
     1501 ttcactctca atcagatttg attatgaatg aaaacttggc ccttgggcaa acgactccat
     1561 ttttgggaag taaaagttgg atatctctaa aattgtggtt agtagctaaa catttaggat
     1621 cagagaaaat aggagcgatg gtagacaaga gaatagagtt aacgaattat ttgcaagatc
     1681 taatacttaa gagttctaag ttaatattat taaataggac agatattaat tcagttatgt
     1741 tcatgtatat aggtgataaa catcaagaag taagcttatc atcagtacag aatataaata
     1801 acattaataa gagtatttat gacagactaa aagatgaagg aatatactac ctacatcaat
     1861 ttccaataat agataataca gaaaccatat acagagggtt tgaattatgt ccattacgat
     1921 ttatgtctgg aaatcctaat ttaacaattg aagaactaca aaaaatggtt atagaagtag
     1981 aaaatctagg ggatagattt gtaggagaga gttaaaaaat gagtaaagag ctctataata
     2041 aatttatttt gacaaaggaa gctggaaata taatatatga cggcattgaa tttggcaggg
     2101 atgaaattga taggagaatt ctcgaaattt ctaaaatact tactgcagat attgtcaact
     2161 tatcacaaga agcagttgct ataagttgta ataaaaaaat aaactttttc tttctttttc
     2221 tggtagttaa ttgttgtgat ggtgttgtta tgccagtgta tgctagaacg ccagaaaata
     2281 aattacaaca tatctgtgaa aggtatgaca taaactatat ggtttctgac catgaaatta
     2341 atttggtagg gtatagttta atatatcatg ggagtgtttt ttcagaagag tattttatat
     2401 acgagtgtaa taaatcattt ccgaaacatg aggagttgaa ggggattgaa ttgttaatgc
     2461 tctcttcagg tactacagga gtttcaaaag cgattagttt atcgcttgat aatattttat
     2521 ctaatactag aagcattcaa aaatatatta ttcctactag agaagatagg tgtctcataa
     2581 taaaagaaat cacacactcg tcgtcattga tatcagaatt aataaatagt ttgttatcaa
     2641 aaaattcagt gttgttaagc gatttaattc cgaccgttag ttcattaggg tatttaataa
     2701 gtacatacgg tgtgactatt ttatacgcta tacctgattt tctattgttt atgaactcta
     2761 ttaaatttat aaataattat agacaattgt tagggaaatt aagattagta caggttgtag
     2821 gtgcattttg tcctccaaat tatataacag attttcataa aaactggcca aaaattgatt
     2881 tatttaatgg ttatggttta acagaggcct ctcctagagt tgctgtatta ggaggagaag
     2941 aattatatgc aaatcctagg tgtgtaggtt atccaatttc aggagttggt gtgcatattg
     3001 atacaagtag caacaacacc caagagggag aaatttgtgt tactggtcct aatattatgg
     3061 ttgaatatta cttgaacgaa gagcttactt ctaaaataaa aattggaggt atgttaagga
     3121 ctggagacat tggttacttt gatgataaaa acaggttata tattttaggc agaaaagaca
     3181 ataggtatat aaaatcagga aaaaatatat ttccggaaga aatagaatct gtagtaagaa
     3241 aattaccttt tatatccgaa gtaatagttt atcctaaaat agaatctggg agattaatat
     3301 ctattcttga atatactatt tgcaatgatg aagagaaaga tttgaatcaa gaagcaagtg
     3361 aaataataaa attatgcaaa gataatctag aagattataa aatccctgat caatatagga
     3421 aggttaaaaa tatcccgcac acttctacag ggaaagtaaa acgtgatagg aataattatt
     3481 ataatgaatg agctatgtaa aaaatatgtt tatcaattac aaaacgaact aaatcagtat
     3541 tgtgataatc tggagggaat ccatgttatt gcttacggtt caaatggtag taatgatttg
     3601 tctatttcat ctgattttga tgtttgtctt attgttaacg atgattcatt tgttgctatg
     3661 aatcagcggt tgatatgtga ctttacaaag ttatttcatt ttcaagaaaa tttaccgata
     3721 gataaggata ttcctttgag caacaaactt atttatctaa gcagtgaggt tgaattattt
     3781 ttggataatc cgccgttcat atcagattct aagaaagtta agattgaaga tattaaaaaa
     3841 acgaagaact ttttaacttc aagaactatg aaacaacgtc tcttactaaa tattttgact
     3901 acagattcta cagttttatt tggtgatgaa gattatgtta ataatctgaa atatagggca
     3961 tgggaggaca ttgaaaaaat aattgaaaga gcgtataata ttaaattaac gaccaaaaat
     4021 gaaaatgaat tcttaaattt actatatcaa aacccagtat cgaaagcaag tggagagtat
     4081 tatttaggat ataaaaaaga aaaaaggaat gttgttaaac atttagaaag gaatgttcac
     4141 cgattagcaa ttttgaacga gaaaaaggtg gaagatataa ttgatttatc tgaaaacgtc
     4201 aataggttac tcttgaaaga ttactcattt ttatttcctt ctgaaactat aaaaaaaatg
     4261 ataagcacat atccagaaaa aaaatcatgc agtattaaag aagttttgtc ttctaaatta
     4321 acaatagcca gtaacaattt cttgattaca aacggttctt tagagggtat gaatattata
     4381 attgcgaact tggttcaaaa taatatagga tatttaaccc ctactttttg gggatatggg
     4441 gattctgcaa aaaggtataa taaatcagtg atttcggcac ctttgtcaga tatttataat
     4501 tataatgtta cagaaataga taaaatttct aaatcttgtg agcttatgat aatatgtaac
     4561 ccaaataatc cgactacggc tactttaaca aaaaatcaaa tagaaaatct tcttttaaac
     4621 aatccacaaa catatttttt gattgatgaa agcatgttgc catataaata tgattttttt
     4681 aattatacaa tgataccatt tgtagaaaaa tatactaatt tgattgtttg ttcctcaata
     4741 tcgaagtcac tatgtgctcc tggatta
//
LOCUS       DAVKGU010000111         4607 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKGU010000111 DAVKGU010000000
VERSION     DAVKGU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.113"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(56..1066)
                     /locus_tag="VW077_001906"
     CDS             complement(56..1066)
                     /locus_tag="VW077_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9455859.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VW077_001907"
     CDS             1215..2384
                     /locus_tag="VW077_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9455860.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VW077_001908"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VW077_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9455861.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQNLTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VW077_001909"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VW077_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9455862.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPMRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTIDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VW077_001910"
     CDS             4146..4379
                     /locus_tag="VW077_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9455863.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            4425..4607
                     /locus_tag="VW077_001911"
                     /pseudo
     CDS             4425..4607
                     /locus_tag="VW077_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1356 a          881 c         1173 g         1197 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtattaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaatgagga atttagagag ttgtttgaga ggggtaaaga
      241 aactactaga cacaccgaga gaggtcagaa gggtggttag gagcgatgca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggataga aagagcgtga tagggataat ggccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatcaata acgggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttataggac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatacg atagaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggcta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcgg
     1441 ccagtgattt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaatcttgg
     1501 tcactatcgg agcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcgaccttca
     1981 cagcccagtt aatcaagagt caccagtact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaacgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaaat ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tatgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggcagcagg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggacttatgt caacattgcc gactatagat
     3541 gggaaaggtt ttgacatgct tgacctcggt gctaatgcag aaaatacagc ccagcacctc
     3601 catcaatacg ctgtcctagg ttctttctat gccaagaatg ttcgtggcat tgcgcaacca
     3661 cgtgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagtggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgccagatt
     4081 cgtaccatgc tagaaacaga cgtagttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aagaaattga ccaactccaa aacgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aatgagaaat atcggacaag
     4441 ctggaaaaat cttggctgac agtggttatc aagggctcat gaagatatat cctcaagcac
     4501 aaactccacg taaatccagc aaactcaagc cgctaacagt tgaagataaa gcctgtaatc
     4561 atgcgctatc taaggagaga agcaaggttg agaatatctt tgccaaa
//
LOCUS       DAVKGU010000112         4552 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKGU010000112 DAVKGU010000000
VERSION     DAVKGU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.114"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(116..391)
                     /locus_tag="VW077_001912"
     CDS             complement(116..391)
                     /locus_tag="VW077_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9455864.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(498..1337)
                     /locus_tag="VW077_001913"
     CDS             complement(498..1337)
                     /locus_tag="VW077_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9455865.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1479..2327
                     /locus_tag="VW077_001914"
     CDS             1479..2327
                     /locus_tag="VW077_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9455866.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2420..3337)
                     /locus_tag="VW077_001915"
     CDS             complement(2420..3337)
                     /locus_tag="VW077_001915"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9455867.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVEQLKADEEVTRNWS"
     gene            3775..3980
                     /locus_tag="VW077_001916"
                     /pseudo
     CDS             3775..3980
                     /locus_tag="VW077_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4252..>4552)
                     /locus_tag="VW077_001917"
     CDS             complement(4252..>4552)
                     /locus_tag="VW077_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9455868.1"
                     /translation="LIKNEVRMMISCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLHV
                     VGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPIPYEPPGRKASLMMH"
BASE COUNT         1399 a          926 c          746 g         1481 t
ORIGIN      
        1 atacaatatt ttcggaacat tcaacttttt aactctattt attactagat ttcataatta
       61 aaaagcctac tgaccaagct agaaagcttg atacaatagg ctttttaaag actgattatt
      121 taacagcgtc tttaagagct ttaccagctt tgaatgctgg tactttagaa gctgcaattg
      181 tcatttcttt accagtttgt gggttgcgac ctttacgttc tgcacgctca cgaacttcaa
      241 agttaccaaa accgatcaac tgaacttttt caccagctgc aagatagtca gctactgctg
      301 caaatacagc ttcaactgct gctgctgagt ctttcttagt caattctgta gcttctgcta
      361 ctttagcgat caaatcttgt ttgtttgcca tgttaatgat tcctccaaat aatttctaat
      421 taacaaatat aatcatatcc taaaatccct tttaggtcaa gttaaaaacg ctatttcttc
      481 ccattttatt tattttttta ggagtggtaa cgtatcaaaa tagcccaagc gttctcaccc
      541 gtgtgagttt gaataatgga acccgtttcc aaaacagaaa ttggcttttc aacataagct
      601 tgtaagcttt ctttcatctc ttttgcccaa tcatcactac cagaatatga aattccaatc
      661 tctgctacag cacgttcaga aagcgatgtt atcaactcat ctaaccattt tttaaatgtt
      721 ttagttccac gacctttaac cattggctgc aattcatggt ctttcatttg catgacaaca
      781 cggatattga gaagagagct caacaagcca gttacacggc taattcgtcc acctttgaca
      841 agattttcca aagttgaaac accaatataa agctctgtat ggtttttaac ctcttctaca
      901 tgagataaaa ttgcctccat atctttacct tcttgagcta acttcgcagc ctcaacaact
      961 tggaatttca gggcttggtc agtgaaggaa ctatcaacaa cagtcacatc tgcagtagat
     1021 aggctagcac cttggcgtgc tgcttctacc gtacccgaaa gagcatggga catatgaata
     1081 gcaagaatct ggccaccatc tttgcatagg tcttcaaaaa tctcagcaaa gacacctaca
     1141 gggggctgac ttgttttcgg aagattctta ctttcttgca tcaactgaag aaatttacct
     1201 tcttctttca aatccgcatc agaataaaca acattatcaa tcattacaga taatggaaca
     1261 attgtaatat ctaattgctt tactagttca ggttcaatag taacagatga atcggttaca
     1321 atcttaattt ttgtcatagt atcaatcttt ctattttagg attcagattg gtttccttac
     1381 ttctaattat atcaaaaaaa agattaaaaa tcctaatgga gtcaatcaaa ttttccgtaa
     1441 aatttgatat aatcaactta taagaaaaga ggtgtcctat gattaaaaaa atttacccca
     1501 tttttaccat tttactaggt gctgctattt atgcttttgg actgacttat tttgtagttc
     1561 cccatcatct ctttgaagga ggggcgacag gcattaccct catcaccttt tatcttttta
     1621 aaatccctgt ttccctcatg aacctgctga ttaatattcc ccttttcatc ctagcttgga
     1681 agatttttgg agccaaatcc ctctattcta gtttactagg aaccttagct ttgtccggct
     1741 ggttagcttt ttttgagcat attccccttc atattgatct tcaaggtgat ttactaatca
     1801 cagcccttat agcgggaatc ctattgggaa ttggccttgg aattattttt aatgctggag
     1861 gtacaactgg cggaactgat attctagctc gtattctcaa caaatacact catatatcca
     1921 taggaaaact gctctttatc ttagattttt gtattctcat gttgattctc ctaatcttca
     1981 aggatttgag attggtttcc tacacgcttt tgtttgattt tattgtttct cgtgttattg
     2041 atttgattgg tgaaggagga tatgccggca aaggctttat gattatcaca aaacgtcctg
     2101 accaacttgc taaggcgatt aatgatgacc tcggaagagg tgttactttt atttctggtc
     2161 aaggctacta tagtaaagaa aatttgaaaa tcatctactg tattgtcgga agaaatgaaa
     2221 ttgtgaaaac gaaggaaatg attcatcgaa tcgatcctca agcctttata actattacag
     2281 aagcccatga aatcctagga gaaggcttca cctttgaaaa agaataaaaa gaggtagtgt
     2341 cgtgacctca aaagttagac taaatcatct atcttttggg ttacagacaa cctctttttt
     2401 gttttattta ctcaagctct taagaccaat tccgagttac ttcttcatca gcctttaact
     2461 gttccactaa ttggtcaact gagtcaaatt tggtcatatc acgaatgcga tcaagccaat
     2521 aaaccatgac ggtttcccca taaatatctt gattaaaatc aaaaatattg acttcaaaac
     2581 gtgcttcttc tccatcaaag gtcacatttt tcccgacact agccatagca cgatacttct
     2641 gtctttgaat ctcaacatca acgacataaa cgccatctgc tggcatataa gtacggtcta
     2701 aaagcactaa attcgctgtc ggataaccaa ttgtacgacc acgagcatta ccatgaacca
     2761 ccatacctct tgatggaagc ggtgccccca aaagttttcc tgcttctttc acatttccat
     2821 ctaaaatagc ttgacggata cgagttgaac taatctttcc tttctcatct tctacaggtg
     2881 gaacaatgat aacttctcca tcaaagtaat cctttaaatc ttctgctgtt tttttgtcag
     2941 aaccaaatgt ataatcaaaa cctgcaacaa taattttggc attcatagcc ttgatataag
     3001 ttgcaaagaa ttcttgtgca gtgagactag cgaattgact actaaaatca aggagatata
     3061 attcttctac accttcgcgc tttaattttc tttcacgttc agcagggttc aaaatatgca
     3121 aaaacaaatc tggatgataa ggctctaaag cgatctttgg agattcatta aaggtcataa
     3181 cgacgatagg taataaatcc tttctcgcag ccttgttggc aacacgaaat aattcttgat
     3241 gccccttatg tatgccatca aaatagccga gaacaacgac tgaatcagat ggtgtgccaa
     3301 tatctttttg gttttttata ggaatagtaa taatcataaa ataattatat catagcgata
     3361 gctatttctg gaacagaaaa tctgaaatgt tgtttttttc acatgaagtg tacctgtttt
     3421 caaaaagcac tttattctat cgttgcttaa ctatgaactt tgcaatattc ttctcaaaaa
     3481 cttgtaggac atcttcaaaa ttttgcaagg agtgattaga cttgttcggt aaccataaag
     3541 tgtcatacta tgcttatgta tgaaaaagca atgcaactaa ctcctgagaa ctttaaatta
     3601 ctaattggtg ccgaaaaggt agaatttaga atcgaggtac acctatggct gtaaaattta
     3661 caaaatgaga caacttgggc aagatgtttg aagaatttcc taaactccct gatttgaagc
     3721 aagtcacttt ccctgatgac aaagaaaaaa gccaaaacag caaagaaaaa ctagatgact
     3781 gctttccaac aactcccatc tagtgtgctt cagactaggc tatttttctc tccatctgtt
     3841 agcttggatt ctcagaccgt ttcagctaaa gaatatcttt tcccttatca gaaggaacgg
     3901 ctcaaaccat tcagacaagt gaagggacga caagccaata tttgaaacca gatagcagtt
     3961 cttatagtca attgaaataa aatctgaaga aatcgagtag gaaactcata tcaattttta
     4021 acagtgttct attccagatt catactcaat gaaaattaaa cccaaatcat tcatacctct
     4081 ctcaactaga tgtaacttac aaaacccctg acctcatgag ccactttctt cctcctcatg
     4141 aggtcagttt tactttctgc tgttccagta tcgtttttcc tcgctagatt tcctcaaaag
     4201 ggcagactcc tcccttggtg cgtcacacga ttttttcatc tcgactgttc tttaatgcat
     4261 cattaacgac gcttttcttc caggtggttc ataaggaata ggaagattca ggttgacttt
     4321 tctaatccta gaataaagtg ctgaaaacaa ttcggaatag gcatagagac tagacaattt
     4381 gaggagctgc ttgcgtcctg ttcgaacaca ttttcctacc acgtgaagaa aaagatggcg
     4441 gaagcgtttg attgttaaag tttggaagtc acctccagct agatgtttga gaaaaagata
     4501 gagattgtag gcgatacagc ttatcatcat acgaacttcg tttttgatta ag
//
LOCUS       DAVKGU010000113         4458 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKGU010000113 DAVKGU010000000
VERSION     DAVKGU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.115"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            174..260
                     /locus_tag="VW077_001918"
                     /pseudo
     CDS             174..260
                     /locus_tag="VW077_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(641..838)
                     /locus_tag="VW077_001919"
     CDS             complement(641..838)
                     /locus_tag="VW077_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455869.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
     gene            complement(823..1101)
                     /locus_tag="VW077_001920"
     CDS             complement(823..1101)
                     /locus_tag="VW077_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9455870.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(1140..1925)
                     /locus_tag="VW077_001921"
     CDS             complement(1140..1925)
                     /locus_tag="VW077_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9455871.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(1927..2745)
                     /locus_tag="VW077_001922"
     CDS             complement(1927..2745)
                     /locus_tag="VW077_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9455872.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(2747..3739)
                     /locus_tag="VW077_001923"
     CDS             complement(2747..3739)
                     /locus_tag="VW077_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9455873.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT         1506 a          940 c          759 g         1253 t
ORIGIN      
        1 aactttgcta gcctttgtta taaaaagttt tactaagtat tatctaggaa atagagtagt
       61 acatttatat ataattgtta tctctccata aaaacagtat atcatttaaa aaaatttaat
      121 tcaagaaaaa ttaacatgca ttaatttatt ttttagcaca cgttaaaaaa taagattagt
      181 actcaatgaa aatcaaagag caaactagga aactagccgc aggttgctca aaacactgtt
      241 ttgaggttgt ggatagaact gacgaagtca gtaaccatac gcacggcaag gcgacgttga
      301 cgtggtttga agagattttc gaagagtata agtccaaaac tttcgttaac cgggatacat
      361 tataagcaca ttcataccat caaaaatagt agcaataaac ttgaacaact accagaaaca
      421 cgaaaacagg cataattgat gaaatatcag cgctattaaa caatataaat aaccgctaag
      481 aataaggctt aacggttatc ttctacctat ctattttttc aactcctcag tcgtttatct
      541 acctataacc taggctttac gagtgccttg ctctaccaac tgagctaaat cggcgattac
      601 acttacagta tggcactctg agaatagatg tcaagtaata ttattcaagc tttttaatca
      661 aaatctgaca aggattttgc ggattccata gttgaggaaa aatctctaac tttttaaagc
      721 caagacctcg ataaaagtca tttgttcgat cataatcttt attagaacct tctgccactg
      781 ttttgacctg cagataacca acttttttac gagcttcact ctctaaagta gcaagcaatt
      841 ggctcccaat ttttctacct tgataagctt ttttacgccg agacaatcaa tctctgcaca
      901 atcttcactc gaataggata agcttacaaa tctagtcaaa tcactctcct gataggcggt
      961 ccaaacttgc agtgtcgtgg ttccttctat ataggcttgt gtgctttctg ggattccaaa
     1021 ccattctggc aaatccttca aaacctcagc tacaactgcc tttttttgat tttcatcctt
     1081 gacttcttca attacaaaca tcataagttt ctttcttgat tttagtctta ctttagcttt
     1141 tacgaacccg cactttcgta ggaatctaag cccttatccc aaagtttaag ggaaataaca
     1201 aagaaaacca gagaaatcaa catcaaacct cctacgttaa agaacacatc cttttcctgt
     1261 aagaaatagc tagctggata gtaggctgta aaggcgaaag gcacgataaa gctaatcaac
     1321 caacgaagaa gagaattgta aatagaaatc ggatacttag caaagtcatt gaacatatag
     1381 aagatgtaaa tcatggcgcc tgactgctta gtccaaaagg cgatactggc tgttgcgatt
     1441 ttaagagaag tataaatcaa ggtcgcaaaa ggaatacaaa ctaggaaaag caggaatttc
     1501 ggaagagtcc aaacaatgct ggtcactgtt gttcccaata aaataccacc gactaagagt
     1561 tcacccaagg catcaatctg aaaggtttca actaggatgt gaaagagagg attgatggga
     1621 cgagtcagat acttgtcaaa ctctcctttt cggactaggc gttgccctag tgcccagaga
     1681 ttgtcaaaaa agagatggtc cattcccttg ggaatcaagg aaaatccata aatgaaagct
     1741 atctcttgaa aggtccaacc ttctaggaat ggaatatgtt gaaagatgac attgagaaac
     1801 aagagattca agccttgagc cagaaagact cccaagacac caaccacaaa atccacctta
     1861 tattccatga tttgtttgat gtattgtctg ataaaaatca gatgcattcg ttgatatttt
     1921 ttcatactaa cctccttgaa tggtgataaa ggactggacc cgtttccaaa tcaactgaga
     1981 caatcccacc atcactaaga gccagaagaa ctgcaaaagg agtgcctgaa gaatctgact
     2041 ggcatcgtat tttccaacaa tgatcataac tggagtataa atcaaggatg aaaaaggcaa
     2101 aaaggaaaga atatctgaaa caacctttgg aaagaatgcc aagggaatca aactccccga
     2161 cataaaagcc actatggaag tcttaagtag gttggaaccc caaagatttt taaacacaaa
     2221 ggctgaaaat ccaaagcaaa tattaaagaa aaagttaatc agataggcga gcgttaagct
     2281 aaaaagataa aggacagtta atcctagcac ctctacaata ccttgacccg atatgatttt
     2341 catcaagaca atgacactta aaaatggaag gccaacgctg ataaaaatca accacttgga
     2401 accaagctcg gtgaaaagat aggaggccgc aaaatgcact ggtcgcaaca aacgcatgat
     2461 aatggagcca tccttgacct cctccccaat cataaaggac gaatcggatc tagtcagaag
     2521 attggtcaca aaactcatga tgatgtagag ggtgatatcc gccatactga agccctgaat
     2581 caaagactct tgcgaagaat caaagacagc cttccagaga taaaaggcca caaaagcccc
     2641 catgacatcg ccaatccgat agagaataaa gttgactcgg taagtaatca actcctgaac
     2701 ccctgcattg ataaagggtt tataacgtct ccacaatttg atcatcctag agctcctttc
     2761 ggtagaagcg acggataata tcctcaatat ccgtatccac catcttcaaa tcgcggattt
     2821 caaaatcaga cagggtttgc ttgataatgt cagctgactg gtagcgagaa ctatcaaatt
     2881 caatgttgag gctgtttcct tgtctatcaa tggtcatatc agacagacct tcatagtgag
     2941 agacgagatg actttgacct ggtagcagtt caaaagagag agtcttcatc ttaccaaagg
     3001 tctccttgag ttggctcacc gttccatcaa aaatctcttg ccccttgtca atcatgaaaa
     3061 tccgatcaca aagttgctca atatcactca aatcgtgagt ggtcaaaaga atggtagttt
     3121 cttcctcttg attgatctga gtaattgccc gacgaatatt atccttaacc gaaacgtcca
     3181 aaccaatggt cggctcatct aaaaaaagaa ccttgggatt gtggagcaag gaggccgcaa
     3241 tatccgcccg catccgttgt cccagtgaaa gagtccgcac gggatccttg ataaagtcct
     3301 tcaaatccaa gacttcattc aaaaagtcca tgcgcttatg aaagagcgag tttggcacat
     3361 cataaatctc ttttaagaca gtgtaggtct cttgcagagc caaatcccac catagctggg
     3421 tgcgttgtcc aaagactacg ccaatatctt tgacataatc ttgccgattg tcctggggaa
     3481 tcttgccgtt aatccgacaa aaaccagatg ttggtttcaa aattcctgtc agcattttaa
     3541 tggttgtcga cttcccagca ccatttgccc cgataaatcc taaaatctgc ccttttggaa
     3601 cctcaaaggt caaatccttg accgcttcaa aggtctgctt ttcaggatga ataaaggagc
     3661 gcaaagcccc cttcaagccc ggttccttaa cagtcttcac aaaatttttc tgaagatgtt
     3721 ccacttctat cattgccata tctatctccc tatcgtaagg aatagcaaca ttgtgttttt
     3781 aactacaaat attctaaatc cttgctttct acaccttcta cattttatta ctacaaaagg
     3841 ctttatacca acctattata gtccaataaa aaacgaaatg caagagtttg cctatggatt
     3901 gtgaaattag gatttctctc ataaaataat acttttaatc aaaaattctg tttaatagtt
     3961 taaattacca acctactcat cttctcacca gagggcggta ttaactttga tcataaaatc
     4021 ggatactccc tctatccaga aaatatgctc agaatgattg tctctaaaca atacctgctc
     4081 ttttacctca ttcatgaaaa agaagtccat accctgagaa ttatcaactc acgcatcgac
     4141 tacctaaacc aactcgacca cctctttcgt acatgtagga aactattttc ctcacaaata
     4201 atctcattat aaaatccaat aaattttttt agctcacttg aatattttct ttgtatcgtt
     4261 ttatcagctt caatctgatc tagacttgtt accttggaac gacttgaaat aacctttata
     4321 aaataacact taaggctttc atcatctcgt ataaattttt caatagtgat ataaaaagct
     4381 ttcatattct gtttgtaatc agatatcaat tgattaattt tatcattctg atgaagattc
     4441 gataccataa tatatagc
//
LOCUS       DAVKGU010000114         4442 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKGU010000114 DAVKGU010000000
VERSION     DAVKGU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.116"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            48..4439
                     /locus_tag="VW077_001924"
     CDS             48..4439
                     /locus_tag="VW077_001924"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9455874.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFETKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGIEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFINKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEAIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
BASE COUNT         1239 a          836 c         1136 g         1231 t
ORIGIN      
        1 acctctaaat atggtaaaat agtagaagaa taatgtgagg aaaatgaatg tcaaatagtt
       61 ttgaaatttt gatgaatcaa ttggggatgc ctgctgaaat gagacaggct cctgctttag
      121 cacaggccaa tattgagcga gttgtggttc ataaaattag taaggtatgg gagtttcatt
      181 tcgtattttc taatatttta ccgattgaaa tctttttaga attaaagaaa ggtttgagcg
      241 aagaattttc taagacaggc aataaagctg tttttgaaac taaggctcgg tctcaagaat
      301 tttcaaatca gctcttgcag tcctactata gggaggcttt ctctgaaggt ccatgtgcta
      361 gtcaaggttt taagtccctt tatcaaaatt tgcaagttcg tgctgagggt aatcagctat
      421 ttattgaagg atctgaagcg attgataagg aacattttaa gaagaatcat cttcctaatt
      481 tagccaaaca acttgaaaag tttggttttc caacttttaa ctgtcaagtc gagaagaatg
      541 atgtcctgac ccaagagcag gaagaggcct ttcatgctga aaatgagcag attgttcaag
      601 ctgccaatga ggaagcgctc cgtgctatgg aacaactgga gcagatggca cctcctccag
      661 cggaagagaa accagtcttt gattttcaag cgaaaaaagc tgcagctaaa cccaagctgg
      721 ataaggcgga gattactcct atgatcgaag tgacgacaga ggaaaatcgt ctggtatttg
      781 aaggggttgt ttttgatgtg gagcaaaaag tgactagaac aggtcgtgtt ttaatcaact
      841 ttaaaatgac ggactatact tcaagttttt ctatgcaaaa gtgggttaaa aacgaggaag
      901 aggcccagaa gtttgacctc atcaagaaga attcttggct ccgagttcga gggaatgtgg
      961 agatgaataa cttcacacgc gatttgacta tgaacgtaca ggatctgcag gaagttgttc
     1021 actatgagcg gaaggatttg atgccagaag gtgagcgtcg ggttgagttt catgctcata
     1081 ctaacatgtc gactatggat gctttgccag aggtcgaaga gattgttgca acagctgcta
     1141 agtggggaca caaggcggtt gctatcacag accatgggaa tgtccagtcc tttccacatg
     1201 gctataaggc ggctaagaaa gcgggaatcc agctgatcta tgggatagaa gccaatatcg
     1261 tggaggaccg tgtccctatc gtctataacg aagtggagat ggacttatca gaagcaacct
     1321 acgtggtctt tgacgtggaa acgacgggac tttcagctat ctataatgac ttgattcagg
     1381 ttgcggcctc taagatgtac aaggggaatg ttattgctga atttgatgaa tttatcaatc
     1441 ctgggcatcc cttgtcagct tttactacag agttaactgg aattacagat gatcatgtca
     1501 aaaatgccaa accactagaa caagttttgc aagaattcca agaattttgc aaggatacgg
     1561 tcctagttgc ccacaatgct gcctttgacg ttggctttat gaatgctaat tatgagcgtc
     1621 atggtcttcc aaagattagt cagccagtta ttgatacgct ggagtttgct agaaacctct
     1681 atcctgagta taaacgccat ggtttggggc ctttgaccaa gcgttttggt gtggccttgg
     1741 aacatcacca catggccaac tacgatgcgg aagcgactgg tcgtctgctt ttcatcttta
     1801 tcaaagaggt agcagaaaaa catggtgtga ccgatttagc tagactcaac attgatctaa
     1861 tcagtccaga ttcttacaaa aaagctcgga tcaagcatgc gaccatctat gtcaagaatc
     1921 aggtaggtct aaaaaatatc tttaagctgg tttccttgtc taataccaag tattttgaag
     1981 gagtgccacg gattccgaga acggttctag atgcccatcg agagggcttg attttaggtt
     2041 cagcctgttc agagggtgaa gtttttgacg tggtcgtttc tcaaggtgtg gatgcggcgg
     2101 ttgaggtggc caagtattat gactttatcg aggtcatgcc accggctatc tatgcgccct
     2161 tgattgccaa agagcaggtc aaggatatgg aggaactcca gaccattatc aagagtttga
     2221 tagaggttgg agatcgcctt ggcaagcctg ttctggctac gggaaatgtt cactatatcg
     2281 aaccggaaga agagatttat cgtgaaatta tcgtccgtag tttgggacag ggtgcgatga
     2341 ttaaccgaac tatcggtcat ggtgaacatg cccaaccagc gccacttcca aaggctcatt
     2401 ttcgaacgac taatgagatg ttggatgaat ttgccttttt gggagaggaa ctggctcgta
     2461 aactggttat tgaaaacact aatgccttgg cagaaatatt tgaacccgtt gaagtcgtta
     2521 agggtgactt gtatacgcct ttcatcaaca aggctgaaga aacagttgct gagttgacct
     2581 ataagaaagc ttttgagatt tatggaaatc cgctgccaga tattgttgat ttgcggattg
     2641 aaaaagaatt aacatccata ctggggaatg gatttgctgt gatttatctg gcatcgcaga
     2701 tgctggtgca acgttctaat gaacggggtt atttggttgg ttctcgtggg tctgtcggat
     2761 ctagtttcgt tgcgaccatg attgggatta cggaggtcaa tcctctctct cctcactatg
     2821 tctgtggtca gtgtcagtac agtgagttta tcacagatgg ttcgtacggt tcaggatttg
     2881 atatgcccca taaggactgt ccaaactgtg gtcacaaact cagtaaaaac ggacaggata
     2941 ttccgtttga gaccttcctt ggttttgatg gggataaggt tcctgatatt gacttgaact
     3001 tctcgggaga agatcagcct agcgcccact tggatgtgcg tgatatcttt ggtgaagaat
     3061 atgccttccg tgcgggaacg gttggtacgg tagctgccaa gactgcctat ggatttgtca
     3121 agggttacga gcgagattat ggcaagtttt atcgtgatgc agaagtagaa cgcctcgctc
     3181 aaggagcggc gggtgtcaag cggacaacag gccaacaccc ggggggaatc gttgttattc
     3241 cgaactacat ggatgtctac gattttacgc ctgtccagta tccagcagat gatgtcacgg
     3301 ctgaatggca gaccactcac tttaacttcc acgatatcga tgagaacgtc ctcaaactcg
     3361 atgtactggg acatgatgat ccgaccatga ttcgaaaact tcaggatttg tctggtattg
     3421 accctaataa aattcctatg gatgacgaag gcgtgatggc actcttttct gggactgatg
     3481 tgctaggggt aacacctgaa caaattggaa cgcctacggg tatgttgggg attccagagt
     3541 ttggaacaaa tttcgtacgt ggaatggtag acgaaaccca tccgacaacc tttgcggaat
     3601 tgcttcagct gtctggtctg tcccacggta ctgacgtttg gttggggaat gctcaggatc
     3661 tgattaagca aggaatagcg gacctatcga ctgttatcgg ttgtcgggac gacatcatgg
     3721 tttacctcat gcatgcgggt ctggaaccta agatggcctt taccattatg gaacgggtac
     3781 gtaagggttt gtggctaaag atttcagaag aggagagaaa tggctatatc gaagccatga
     3841 aggctaataa ggtgccagag tggtatatcg aatcctgtgg gaaaattaag tacatgttcc
     3901 ctaaggccca tgcggcagcc tacgttatga tggccttgcg tgtagcttac ttcaaggttc
     3961 accatcctat ttattactac tgtgcttact tctccattcg tgctaaggct tttgatatca
     4021 agaccatggg tgcgggcttg gaggccatca agcgcagaat ggaagaaatc tctgaaaaac
     4081 ggaagaacaa tgaagcctct aatgtggaaa tcgatctcta tacaactctt gagattgtca
     4141 atgagatgtg ggaacgaggt ttcaagtttg gcaaattaga tctctaccgt agtcaggcga
     4201 cagagttcct catcgacggg gataccctta tcccaccatt tgtagcaatg gatggtctgg
     4261 gagagaacgt tgccaagcaa ctggtgcggg cgcgtgaaga gggagaattc ctctctaaaa
     4321 cagaactacg caagcgtggt ggactctcat caaccttggt tgaaaagatg gatgagatgg
     4381 gtattcttgg aaatatgcca gaggataacc agttgagttt gtttgatgag ttgttttaaa
     4441 aa
//
LOCUS       DAVKGU010000115         4421 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKGU010000115 DAVKGU010000000
VERSION     DAVKGU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.117"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(173..1588)
                     /locus_tag="VW077_001925"
     CDS             complement(173..1588)
                     /locus_tag="VW077_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9455875.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1601..3439)
                     /locus_tag="VW077_001926"
     CDS             complement(1601..3439)
                     /locus_tag="VW077_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9455876.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     MKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3457..4296)
                     /locus_tag="VW077_001927"
     CDS             complement(3457..4296)
                     /locus_tag="VW077_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9455877.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
BASE COUNT         1475 a          866 c          579 g         1501 t
ORIGIN      
        1 cccatctatc ctaagttaac tatttataat cggctctctg taacaaaatt aaattaatta
       61 atccccctat aaaataaaga ggtttagaat ttgatgtctt ccaaacctct ttatttcata
      121 tttaatgaga tgttgcctta accgtgataa tatctaatca tcaataaaca aactatttga
      181 ataaggattc tccatttgat tcaatcactc ctttatacca agtaaaggac attttcttat
      241 atcgattgaa tgtgccattt ccatcatcat ttcgatcaac ataaatgaga ccgtaccttt
      301 tagaaagttg tgcagtggac atagaaacgc agtcaataca tccccaagat gtatagccca
      361 taatttcaac accatcctgt agagcttcag caacttgcaa taaatgttct ttcatatact
      421 gaattctata atcatcttgg acggttaagt tattaagttc atcttttatt agttgatctt
      481 tagcacctaa tccattttct actataaata atgggatttg ataacggtca taatatctat
      541 ttaaaattat acgtagtcca attggatcaa tttgccatcc ccactctgaa gactctaaat
      601 aaggatttac taaaccacca ataatattcc cttctcctga attatactgt gttggaagag
      661 cagattgagt cacactcatg taatagctaa aggataaaaa atctacggta taatttttta
      721 ataactctgc atcttcagct gcaaactcta tgttaatgtc atgttcctta aaatatcttt
      781 ttgcataatt cggataataa cctctaacat gcacatctga aaatagataa tttagattct
      841 catactcatg agtcgcccat acatcttttg gatttggagt cattggataa gctggcatag
      901 ctaataccat acaccccacc ttaaactctg aattaatctc acgagcaatt tttgtaacca
      961 aacttgaggc gactaattca tgatgtatag cttgatataa ttcttgtttc gaaagattct
     1021 ccttaggtat atctattcct ccactagtaa atggtaattc caaaacagag tttacttcgt
     1081 taaatgtaag ccaatattta actttatctt tatacctttc taaaactgtt cgagcaaatt
     1141 tttcataaaa atgaatcatt ctcctatcaa cccatccatg atattttctt gctaaatata
     1201 atggagtctc atagtgtgaa agagttacaa gtggctctat cccgtgagca tgtagttcat
     1261 caaacaattc atcataatat ttcaaaccag cttcgttagg ttcttcttca tctccttttg
     1321 gaaaaattct actccatgca atagaagtac gaaaaacatt aaagcccatt tcagaaaaca
     1381 aggatatatc ttccttatat ttatgataaa aatcaatacc tatcaatttt aagttatctt
     1441 ctgtaggatt ttctgttgct tctcctaatc cacctttggg taacacatcc tgaactgata
     1501 agcccttacc atcttcatta tatgctccct ctacttgatt agctgcaaca gctccacccc
     1561 aaagaaaatc atctggaaaa atggtcataa ctttcctcca ttataatatt accagtaatt
     1621 ccttagaatg ctcgattgtc tgattattag gtaatactaa tacatctaga aaatcattgg
     1681 tattcgttac aattactggt gtaactgttt cgtagccttt agtcttgatt aaattcaagt
     1741 ccatttcaaa aatcaactga tttttgaaaa ctctgtctcc ttcttctaca tgactaataa
     1801 aaccttgacc ttttagctca acagtatcta atccaatatg aattagtaac tcaacaccct
     1861 catcactctt caatccaatt gcgtgcttag tcggaaaaat atttgtaatt ttcccatcaa
     1921 atggtgcata aaccttacct tcacttggga taatcgctac tccgtctcca attagtttat
     1981 ctgaaaatgt tttatcctgg acatcgctta acggaatgat ttctcctgat ataggagaaa
     2041 atatcatttt tttatttgaa actccagctt taacttctaa attactagaa ctctcttctt
     2101 catcgattcc aaatatataa gctaatacaa aggtaataac aaccgaaatg accgccacaa
     2161 ttaaagcatt tacaatattt gatggtacat cagaataaat aaattgaggc aacgctatca
     2221 aagatgggac agcaaataga tacgctttaa cactagtaag acctgcaaat aatcccgcta
     2281 atccaccacc aatcatagct gcataaagcg gttttttata ttttaaagtc acaccatata
     2341 atgcaggttc ggtaattcct gcaagtaagg ctgagaaacc tgctgcaaaa gcaatttgtt
     2401 ttgtattatt atttttactc tttaatgcaa cagccatcga agcagcccct tgagctaagt
     2461 ttgaccctaa cattgctgga agaattaata cgtctggagt agcaatagat gccgccaaaa
     2521 aaataggtgc aaaagcccaa tgcattccag tcataacaat aaatggcata atagcaccaa
     2581 gaatagctaa tgtaagccat ccagctacac catacatttg cccaactaga tttgataatc
     2641 cttcaccaac aattactcca ataggtccga ctacaactaa ggcaatacag cttgatacta
     2701 ataatactag cgtaggttgc aaaaaactct tagtaacagc tggtgttaat ttagcaatca
     2761 ttttttcaat atatttcatc aaccaaacca taataagaat tggaacgact gacgaaccat
     2821 aactagctgg tgtcacaggt acaccaaata aactaagagg attccctgat tgcaccattt
     2881 gaacaaaatt tggatggaga agtacacctg ctacagacat agctaatgta gatgttactt
     2941 ttaatttttg tgatgcagaa taagctaata acagcggtaa gaaataatat ggcgcatccc
     3001 caaaaaatgt caaaaaagca atagtctgag aatctgattg caatatacca agcattggta
     3061 aaataattac caagactttc agcatacctc cccctaacat tgctggaatg attggagtca
     3121 tggaaccagc gatatactca atgattcttt ctaaaatatt ccctttgtgc ccttgaacaa
     3181 ctgaatcgga ttcaaaattg ccaagtttaa cgaattcttt ataataatta gctacatcat
     3241 taccaagtat aatttgatat tgtccattct ttttcataat acctattaca cctggtatct
     3301 tcttcatatc atcatcattg actaaatttt catcttttaa ttctaatctt aaacgtgtta
     3361 cacaatgggt aactctattg acattttttt cacctccaat tacatcgagg attttttgta
     3421 ccttatcttt ataactcatg gtattctcct attctattaa tctaaatttt ttgttaagcg
     3481 acgaatatga gccatcaaat aaactaattc actagaagtc agcaaataat tgtactcagt
     3541 ttgtataaac attgccacct gttcaccaca ttcatattct ctaggatatt tatttttcat
     3601 taatgctaac aagtcttcat catcatcgtc acaataagtt gtattttcaa ccaagcgatg
     3661 ggcaaataat tttatatgag ttatgaatct ataataatca atagaatctt ggttaaactc
     3721 agtacaatag aaatcttgaa tgattttctc aattcccata attatttcag tgattttata
     3781 cgactcttga aaattatttt ctaaattagc attaacaaaa tgtagcgcta tgaatgcagc
     3841 ttcgtcaatt ggaagttcaa tacctaactc tttttttatt atttgtaaag cccttttccc
     3901 aagttcaaat tcttctttat aatatttcga tatttcccag cgtaggggat tcgaaataat
     3961 aatcccttct ttataacgtt ctatgctcga agaaatatga tccgttaaat taatatagat
     4021 aatttcatca aaattattac ccaatgttat tttacctagg ttaattattt tttcggtgat
     4081 gtacaccaac tccaaaggaa cgttaataaa catctctgta aaacgggtca tattatctct
     4141 atttttgaga aaaaaacttt tctcaatatc aattggattt actttatctc cagtttttct
     4201 tccaaaagct atgccccttc caaaaagaag aatatcgata tcttttttac tttgcgcaat
     4261 tacggcattg tggtttagta ttcgttttat gatcataaaa tttccctttt aaaataaaag
     4321 gaacctactt aaaaacatca agaaacttct tccgatgtga ttaaataggt tcctgctcac
     4381 ttgtattagt gataacatcc ttttatttca atttttaatg g
//
LOCUS       DAVKGU010000116         4320 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKGU010000116 DAVKGU010000000
VERSION     DAVKGU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.118"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(270..998)
                     /locus_tag="VW077_001928"
                     /pseudo
     CDS             complement(270..998)
                     /locus_tag="VW077_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(1258..1569)
                     /locus_tag="VW077_001929"
     CDS             complement(1258..1569)
                     /locus_tag="VW077_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455878.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1584..2870)
                     /locus_tag="VW077_001930"
     CDS             complement(1584..2870)
                     /locus_tag="VW077_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9455879.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            3494..3676
                     /locus_tag="VW077_001931"
     CDS             3494..3676
                     /locus_tag="VW077_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9455880.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLCFNEKFGTENT"
     gene            3686..4051
                     /locus_tag="VW077_001932"
     CDS             3686..4051
                     /locus_tag="VW077_001932"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9455881.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            4048..>4320
                     /locus_tag="VW077_001933"
     CDS             4048..>4320
                     /locus_tag="VW077_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9455882.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIR"
BASE COUNT         1436 a          817 c          733 g         1334 t
ORIGIN      
        1 aaatgtcatt ttgtaaaatt ttattaacaa aaaactctct acaacaaaat aatagagagt
       61 tttctgctta ttaatttttt tcaaaatatc taagacatcg cttctttgtt taatttgata
      121 ggctttaata cctaagctcc gactttatca cttaaaaaat aaaaaaggac agctaatctc
      181 ttgcgcatca agggatttcg ccattcttta atctcgttta agtgttgtat atggagcttt
      241 gcataatttt ttaaaactaa acaactacat taagcacgaa ctgtgacgct ggttccatct
      301 tccttataaa gattgataag cccctcttta agagctcgaa ccatgtctcc agcttgaaca
      361 ggttgtggga ctttaatagt caatagttcc attggatttg gagcgcggtc gattttattg
      421 cctttggcat catgcaaatc ttcaatatag gtttcaaaat gacggaaacc tggaccataa
      481 aactcaactt ggtccccttc gttaatgacg tttcgttgac gaatagttgc tgtttgtgcc
      541 gcatcatcat aagaaaccac ttcagcgaca aacttgtact cagggatttt acgacgagca
      601 ccaaacaact gctcattttc agatggtgta ccatagtaaa atcctgtagc cagttcacgt
      661 tgggcaacct tccacatctc gtccaccaag tcttgtttga tagcttcaaa cttttcagga
      721 ctttcaagat aggcatccac agccgccttg tagcagttgg ttactgttga tacatagtga
      781 atagactcca tacgtccttc gatttttaga ctgtccacac cattttcaat catatctgag
      841 atatggtcaa tcatagacat atcaacggct gacattgaaa attcttctgg aatctcaccc
      901 tgcaaactct tacgttcttt cccaaatggc atatcgtaaa ggtcgtactt ccaacggcat
      961 gactgagaac atccaccacg gttggcatca cgcatactct agacggattg ttttgacaat
     1021 tctaaccttt tagactttgc ataataactg ttaaacagtc gaaaattacc ccagttttaa
     1081 caagtagtca aaataccaga taaagtaaaa aggggctagg aaaaaagtct ttaaaatctt
     1141 aaaaacgcat agtatcaggt attgaacaat cttgatatta tgcgttttat tgtgggaaga
     1201 tttacttcat tttctcttgg aattgagttt tctcctactt caaaactaga tgatttgcta
     1261 tttaaaatag cgttccttat gattttggca acctggatta aagcgagaaa aacaaaagag
     1321 gcaacttaag ctttctttat attgcttata tgttaatagt tttagacaaa caccacataa
     1381 aataggctta tcctgtatca aagataaggg ataaggctca aaagggtgat tttctaatga
     1441 atcatgacac cgataacaag cataatactt tttacaatca tagcattgta aagcaataat
     1501 atccagcttg ctatggtaat gaatgcatct tgtttgctta tccactaaga aaccctgtgc
     1561 ctctatcatt tccttttcct ttcttaagct cgaactgtga cgctggttcc atcttcctta
     1621 taaagattga tgagtccttc ttttaatgca cgaaccatgt ctcctgattg aacgggttgt
     1681 gggactttaa tagtcaatag ttccattgga tttggagcgc ggtcgatttt attgcctttg
     1741 gcatcatgca aatcttcaat ataggtttca aaatgacgga aacctggacc ataaaactca
     1801 acttggtccc cttcgttaat gacgtttcgt tgacgaatgg ttgctgtttg tgccgcatca
     1861 tcataagaaa ccacttcagc gacaaacttg tactcaggaa ttttacggcg agcaccaaac
     1921 aactgctcat tttcagatgg tgtaccatag taaaatcctg tagccagttc acgttgggca
     1981 accttccaca tctcgtccac caagtcttgt ttgatagctt caaacttttc aggactttca
     2041 agataggcat ccacagccgc cttgtagcag ttggttactg ttgatacgta gtgaatagac
     2101 ttcatacgtc cttcgatttt tagactgtcc acaccatttt caatcatatc tggaatgtgg
     2161 tcaatcatag acatgtcaac ggctgacatt gaaaattctt ctggaatctc accctgcaaa
     2221 ctcttacgtt ctttcccaaa tggcatatcg taaaggtcgt atttccaacg gcatgactga
     2281 gaacatccac cacggttggc atcacgcata ctcatgtggt ttgaaagagt acaacgtcca
     2341 gagtatgaaa tacacatagc tccatggaca aaggcttcaa tttcaacatc tgtacgtttg
     2401 cggatttcag ctaattcttc cattgaaacc tcacgcgcta aaacgacacg agtcaagcct
     2461 agctctttcc agaactcaag ggtttcatag ttagtggcac tggcttgggt agaaaggtgg
     2521 atttcaagac ctggtgcttc agtcgctgca atcataatca aggctgggtc agatacgata
     2581 actgctgcaa tcccgatatc acgcagttta cggaaccact caccagcacc agcttcattt
     2641 ccttcgtgca taaccatatt agccgctaca tagaccttgg caccatactt ggccgcaaac
     2701 tgcacgcctt cttccatctg ttcgaaagta aagtttcccg cacggctacg aagaccatag
     2761 gcctgaccac cgataaagac agcatctgct ccatactgaa cagctacctt tagcttctct
     2821 aaagttcctg caggtgataa aacctcagga cgttttaatg tttttgtcat gatttctcct
     2881 atttgcaata taaaagtttg acatttttcc ttctcatttt atagtaaata aaggataaaa
     2941 tcaagcctag acggattgtt ttgacaattc taatctttta gactttgcat aataactgtt
     3001 aaacagtcga aaattatccc agttttaaca agtagtcaaa aagtctagaa aagtaaaaac
     3061 cagtatccaa agatactgac tcgtaaaaat acataaatta gtccttagat gactactccc
     3121 actctgttgc tcctataatg gaaaaggatt gatacagtct agaatatcgc ttttttattt
     3181 attattctga tataaaactg ttagaattga ttttttgttt tatacccaaa tatgaataga
     3241 gacgtagcta ataagttgaa taacatattt ggaaaaatga gaaatagcaa tattactaga
     3301 acttttagaa acaatcaata tttaaaaaga aaaaagctca aagaatttat taaaccatgt
     3361 tataatagat atagctataa ttttatttat aacaagagga tagaaatgac cgaattagaa
     3421 agaaaaaatc gaaaaattag ctaagaaata ttctgataac ttaaacatca aagttcaaga
     3481 gagagttcgt gaaatggcaa atgataataa gagccattat ttgatataca gagttttagg
     3541 tatttcattt gaagaaggag aaaatatcga tttgtatcaa aataaaggtc gttttttata
     3601 caaatatgct ggttcatttt tagaagaagc tgcagtacta tgctttaacg aaaaatttgg
     3661 tacagaaaat acttaaaaag ttaacattcc taattctgaa agtacaaaac ctaagacttt
     3721 tgaaattgat tgtttagtcg gagaaaaaca cgcatacgaa ataaaatggt gggatgcaac
     3781 tacagatgga gaccatataa ctaaagaaca cactagaata aaagttattc ataacaaagg
     3841 atatatacca attcggttaa tgttctacta tccaaataga actcaagcta taaaaattca
     3901 gcaaacttta gaaacattgt ataacggtat tggagggaaa tattattatg gagattctgc
     3961 ctgggaacat ttaagagcag tgaccggtat tgatttactt agtattctaa cagatattgc
     4021 aaataaaaaa acaggggtaa aatcaaaatg acagtattaa aaggagataa cttagaaata
     4081 ttaaaaacta ttgaatcctc aagtattgat ttaatctata tggaccctcc tttctttaca
     4141 cagaaaaccc aaaaattatc taataacaaa aatattatgt attcattcga agatacgtgg
     4201 acttcgattg aggattacaa agaatttttg tctgtaagat tagaagaatg caaaagagtg
     4261 ctaaaaaata gtggcagtat tttcgttcat tgtgataaaa ttgcaaatca tcatattaga
//
LOCUS       DAVKGU010000117         4319 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKGU010000117 DAVKGU010000000
VERSION     DAVKGU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.119"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            87..1262
                     /gene="mutY"
                     /locus_tag="VW077_001934"
     CDS             87..1262
                     /gene="mutY"
                     /locus_tag="VW077_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9455883.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATASEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1318..2022
                     /locus_tag="VW077_001935"
     CDS             1318..2022
                     /locus_tag="VW077_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9455884.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2015..3364
                     /gene="vicK"
                     /locus_tag="VW077_001936"
     CDS             2015..3364
                     /gene="vicK"
                     /locus_tag="VW077_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9455885.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGESEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     LLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDCARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3366..4175
                     /locus_tag="VW077_001937"
     CDS             3366..4175
                     /locus_tag="VW077_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9455886.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
BASE COUNT         1293 a          761 c         1001 g         1264 t
ORIGIN      
        1 tagatagaaa agttatatac tatctattga aaatattata aatcagatac aaagacaggt
       61 gaaaccttct gtgttatact agagatatgt tagatttgaa agaatacggt atcgtcatgt
      121 ggccggagga gaaagtcatt tctttccgtg agaaacttct cgcttggtat gatgaaaaca
      181 aaagagattt gccttggagg agaagtaaaa atccttatca catctgggta tctgaaatca
      241 tgcttcagca gaccagggtg gatacagtta tcccttacta cgaaagattc ctggactggt
      301 ttccaactgt cgaaagtctg gcaactgcgt ctgaggagag tttactgaaa gcttgggagg
      361 gcttgggcta ttattctcga gttcgcaata tgcaggctgc agcccagcag attatgactg
      421 actttggtgg ccaatttcca aatacctatg aaggaatttc cagcttgaaa gggattggac
      481 cttacacagc aggagccatt tccagtattg cttttaactt gcctgagcca gctgtagatg
      541 gtaatgtcat gcgggtcttg gcgcgtctgt ttgaagtcaa ccacgatatt gggattccaa
      601 gtaatcgaaa aatttttcag gcaatgatgg agatcttgat taatccggat cgtccaggtg
      661 attttaatca agccttgatg gacttagggt ctgatattga gtctcctgta aatcccagac
      721 ctgaagaaag cccagtcaag gactttagtg cggcatatca gaatggaacc atggaccgtt
      781 atccaattaa atctcctaag aaaaagccgg ttcctattta tcttaaagcc ttagttgtga
      841 aaaattctca aggacaattt ttacttgaaa aaaatgaaag cgaaaagctc ttggcaggtt
      901 tttggcattt cccctttata gaagttgata acttttcgca agaagagcag tttgacctct
      961 ttcatcaggt tgcggaagaa agtgtgaact ttggtcccag tccagaagag agtttccagc
     1021 aggactatga cctagatgtt gattggcttg atgtttgttt tgatacagtc cagcatgttt
     1081 ttagtcatcg aaaatggcat gtgcagattg tagcaggtca ggtgagtgac ttccatgatt
     1141 tttcagatag ggaagttcgc tggctttcac cagaagaatt caagaattac ccacttgcca
     1201 aaccccaaca aaaaatctgg caggcttatg cacaagccaa cttagacagt agccaagact
     1261 agctattgtg tcttctgact atttttttgg tataatagtg gagaaaaagg tgaacatatg
     1321 aaaaaaatac taattgtaga tgatgagaaa ccaatctcgg atattatcaa gtttaatatg
     1381 accaaggaag gttacgaagt tgtaactgct tttaatggtc gtgaagcgct agagcaattt
     1441 gaagcagagc aaccagatat tattattctg gatttgatgc ttccagaaat tgatggttta
     1501 gaagttgcta agaccattcg taagacaagc agtgtgccca ttcttatgct ttcagccaaa
     1561 gatagtgaat ttgataaggt tatcggtttg gaacttgggg cagatgacta tgtaacgaaa
     1621 cccttctcca atcgtgagtt gcaggcgcgt gttaaagctc ttctgcgtcg ttctcaacct
     1681 atgccagtag atggtcagga agcagatagt aaacctcaac ctatccaaat tggggattta
     1741 gaaattgttc cagacgccta cgtggctaaa aaatatggcg aagaactaga cttaacccat
     1801 cgtgaatttg agcttttgta tcatttagca tcgcatacag gtcaagtcat cacgcgcgaa
     1861 cacttgcttg agactgtctg gggttatgac tattttggtg atgtccgtac agttgatgtg
     1921 actgtacgac gtctgcgtga gaagattgaa gatacgccca gccgaccaga gtatatcttg
     1981 acgcgccgtg gtgtagggta ttacatgaga aataatgctt gatttactga aacaaaccat
     2041 ttttaccaga gattttatct ttatcctgat tttgttaggt ttcatccttg ttgtgaccct
     2101 cttattactg gaaaatagac gtgataatat tcagttgaag caaatcaatc aaaaggttaa
     2161 agatttgatt gcaggagatt attccaaggt tcttgatatg caaggtgagt ctgaaatcac
     2221 caatattacc aataatttga atgacttgtc ggaggttatt cgtctcactc aggaaaatct
     2281 agaacaagag agtaagaggc taaatagtat tctgttttat atgacagatg gggttcttgc
     2341 gaccaaccgt cggggtcaga ttatcatgat taatgataca gccaagaagc aactggggtt
     2401 ggttaaggaa gatgttctga atagaagcat tttggaattg ctcaagatag aagaaaacta
     2461 tgaattgcgt gatttgatta cccaaagtcc agaattgttg ctagattccc aagatatcaa
     2521 tggcgaatat ttgaaccttc gagttcgctt tgccttgata cgtcgagagt ctggctttat
     2581 ttcaggtttg gtggctgttt tgcatgatac gacggagcag gagaaggaag aacgagaacg
     2641 aagactcttt gtttccaatg ttagccatga gttacggact cctctgacta gcgtaaaatc
     2701 ctatcttgaa gccttggatg agggggcttt gtgtgaaact gtagcaccag actttatcaa
     2761 ggtttctctt gatgagacca accgtatgat gcgcatggtg acggatctcc tccatctttc
     2821 acgtattgat aatgctacca gtctcctaga tgtggaactg attaacttca ctgcttttat
     2881 tacctttatc ctcaatcgtt ttgacaagat gaaaggacag gaaaaggaga aaaaatatga
     2941 gttggtgaga gattatccca tcaattctat ctggatggaa attgatacag ataagatgac
     3001 gcaggttgtc gacaatattt taaataatgc tattaagtat tcgccagatg ggggtaaaat
     3061 cactgtcaga atgaagacaa ctgaagacca gatgatttta tccatttctg accacggttt
     3121 ggggattcct aagcaggatt taccacgtat ctttgaccgt ttctatcgtg tggattgtgc
     3181 tagaagtcgt gcacaaggtg gtacaggtct aggactgtct atcgctaaag aaattatcaa
     3241 acaacataag ggctttattt gggccaagag tgaatacggc aagggttcaa cctttaccat
     3301 tgtgctccct tatgataagg atgcagtgaa agaagaagta tgggaggatg aagtagaaga
     3361 ctagaatgag tgaaataggc tttaaataca gtattttagc gtcgggttcc agtggaaatt
     3421 ctttttatct ggaaacctca aaaaagaagc ttttagtgga tgcaggcttg tctggcaaga
     3481 aaattaccag tctgctagct gaaattaacc gcaagccaga agacctggat gccatcttga
     3541 ttacccatga gcattcagat catatccatg gagtaggcgt tttggctcgc aagtatggta
     3601 tggatcttta tgccaatgaa aagacctggc aagctatgga aaatagtaaa tatcttggca
     3661 aggtggattc ttcgcaaaag catatttttg aaatgggtaa aaccaaaact tttggagata
     3721 tcgacatcga gagttttggt gtaagccatg atgcagtcgc accgcagttc tatcgcttta
     3781 tgaaggatga caagagtttt gtccttttga cagatacagg ttatgtcagt gaccgtatgg
     3841 cgggcattgt cgaaaatgcg gatggctatc ttatcgaggc caaccatgat gtagagattt
     3901 tgcgatcagg ttcttacgct tggcgactca aacaacgaat cctatctgac cttggtcacc
     3961 tttctaacga ggacggtgct gaagctatga ttcggacgct aggaaatcgt actaagaaga
     4021 tttaccttgg gcatttatct aaggaaaaca atatcaagga actggctcat atgaccatgg
     4081 tcaatcagct ggctcaagct gatctgggag tcggagtaga ctttaaggtt tatgatacct
     4141 caccagatac cgcaacacca ttgacagaga tatagaaaga acgctgagaa ggtgttcttt
     4201 ttatggctct ttgtcaactg tagtgggttg aaaaaaagct aagcttgaga aaggacaaat
     4261 tttgtccttt cttttttgat attcagggcg ataaaaatcc gttttttgaa gttttcaaa
//
LOCUS       DAVKGU010000118         4254 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKGU010000118 DAVKGU010000000
VERSION     DAVKGU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.120"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(25..576)
                     /locus_tag="VW077_001938"
     CDS             complement(25..576)
                     /locus_tag="VW077_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9455887.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(926..1750)
                     /gene="nadE"
                     /locus_tag="VW077_001939"
     CDS             complement(926..1750)
                     /gene="nadE"
                     /locus_tag="VW077_001939"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9455888.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADTMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQELGADPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(1747..3207)
                     /locus_tag="VW077_001940"
     CDS             complement(1747..3207)
                     /locus_tag="VW077_001940"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9455889.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3323..3811)
                     /locus_tag="VW077_001941"
     CDS             complement(3323..3811)
                     /locus_tag="VW077_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455890.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAGVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(3801..4010)
                     /locus_tag="VW077_001942"
     CDS             complement(3801..4010)
                     /locus_tag="VW077_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9455891.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1189 a         1003 c          798 g         1264 t
ORIGIN      
        1 ttcgatagaa acagaaaata gaattcaaca ttcttcccta cttataccat aataaataag
       61 atccgcaaga tagccttttc tctctacacc attaacaatg gtttgtagat aggacattcc
      121 agccttttcc atgacacgac ctgaagctgg gttgagactg gcataacgtg ctctgacctt
      181 ttgaaaacct gcttgagtaa aacaaaagtc caagatagct ttcaaagtct ctgtcatcat
      241 accatttccc cagtaagcct tgcctaacac atagccaatt tcacagctta aatcagcctc
      301 gtctatctta acaatgctga tatctcctat cacttgctct gggttttctt ttagacaaat
      361 ggcccattta taatagttga gattagtata ggaagcaacc caattgcaaa tcgagtttcg
      421 agtgatttcg acatcaggat ggggatccca ggtaacatag gtcagattct cagcggatga
      481 agcccaattt tgaaacatgg cttctgcatc actctccaca aatcttctta aaatcaaacg
      541 ttctgtctgt aatatttgcg taccgataga tttcatgatg ctacctcact tccttatttc
      601 tatgctcgta gtaaaaaggc tcaactcagt aacagctaga aaacgagttt gtcttctaat
      661 ttcttgacgc agtcgctgcc tggtttgaaa tgcgctctag caatcttttt caaacctagt
      721 catcgttgca ggggtaagac gatgaaatcg aactcttcga gttaccgaat ctgcctcatt
      781 ttcttatttc aagatgagag gctgaaaacc tccagtggag gtttttcttg ctttttaatt
      841 tcagggcaag aagctaaaaa ggtcccccgg accttcttcg ctttctcatt tctaggctca
      901 gggtaaaaac ctccaatgga ggtttttact cccaaaagtc atcaaatacg gtgatgggta
      961 agtggcgttt gtgttggcct ttgtgccacc agttttcaat ggtcgcttga gcttctgggc
     1021 tgattgtttt gccttctagg taatcgtcaa tctctgcata ggtgactcca agtgcgactt
     1081 cgtcagctag gcctggtttg tcttcttcta ggtctgccgt tgggattttt tcataaaggg
     1141 ctgggtctgc accaagttcc tgtaagagtt gttttccttg acgtttattg aggcggtaaa
     1201 gagggagaat atccgcacca ccgtcaccaa acttggtaaa aaagcctgtg atattttccg
     1261 cggcgtggtc tgttccaatg accgctccgc tatgggaacc agcaagggca tactgagcaa
     1321 tcatacggca acgtgccttg atattcccct tgttgaagtc tgaaacagga ctacctgtcg
     1381 cttcaactgc agctgtcatg gtatcagctg attccttgat attcacaacc aagctgacat
     1441 ctggctggat gaaggctagg gctttttgag catctgcttc atcagcttgc actccgtatg
     1501 gcaggcggac agcgataaat ttgtagctgt cgtctcccgt ttcagctcgc agttcttcca
     1561 tagctaattg cgccaaacgc cctgccaagg ttgagtcttg tcccccagaa atccctagta
     1621 caaaggtttt taggaaggga tgttttttca gatatctttt taagaaatca atagaacgac
     1681 ggatttcttc ctgggcatca atcactggtt tgacacccag ctcttggata atcgtttctt
     1741 gcaaactcat tcttcttctc cttcaccaag ggcttccttg cgcatcttat caatcaagtc
     1801 catcttatct tgccatacat cacgcgccaa atccactgga tagtgctgcg gattgagcac
     1861 acgcttatac tcatcccaca acttgtcaaa ttccttacgg gcataatcct gaatgtcagt
     1921 caaactaggc aagttgtaaa ctaatattcc ttctttgaag atatccacca agagaggaac
     1981 ggcatcaaaa ttacgaaccg tcttcttgat gtatgtatag gtcggatgga acatcttgat
     2041 ttctgtcatg tcgctaatat ccacaccatc ataagtgatg tagtcacctt ctgacttgcc
     2101 tttttcacga ctggtaatgc gccacacctg cttcttacct ggcgtcgaca ctttttccgc
     2161 attattagac agcttaatcg tattgcgcat ctgaccagtt tcatcttcaa ttgcaacaat
     2221 cttgtaaacc gccccaagag ccggctggtc ataggctgta atcagcttgg tacccacacc
     2281 ccagacatca atcttggcct tttgcatctt gaggttaagg atggtatttt catctagatc
     2341 attagaagca taaatcttag cctctgtaaa tccagcctcg tccagttgct gacggacttt
     2401 cttagaaatg taggcaatat ccccagagtc aatccgcaca cccataaagt taatctgatc
     2461 acccagctca cgcgccacct gaatggcagc tggtacaccg atgcgaaggg tgtcataggt
     2521 atccacaaga aagacacaat ttttgtgggt cgcagcgtaa gccttgaaag cttcatagtc
     2581 attgccataa acctgtacca aggcatgggc atgggttccc aaaacaggaa tgtcaaagag
     2641 cttacccgca cgcacgttgc tggttccatt ggccccacca atcaccgctg cgcgtgttcc
     2701 ccagatggcc gcatccattt cttgagcccg acgtgtccca aactccatca agggttcatc
     2761 ttcgataacc gaacgaatac gagctgcctt cgtcgccacc aaggtctggt agttgacgat
     2821 gttcaaaaga gccgtttcga ccaactgaca ttgggctaga ggtccttcca cctgcacaat
     2881 cggttcatta gcaaaaacca aatccccttc ttgggcagaa cgaacggtca actccaactt
     2941 gaaattgcga aggtaatcca agaacgcccc atgataacca agcgactcca aataggctat
     3001 atcactatct gaaaaacgta agtcttcaag atagttcaca attctttcca aacctgcaaa
     3061 aaccgcatag ccgttcttaa aaggctgttg gcggaaatac acctcaaaga ccgccttctt
     3121 attgtgaatc ccttggtcaa agtaaacctg catcatgttg atctggtaca agtccgtgtg
     3181 caatgtcaaa ctatcatctg gatacatact tttcctactt ccttagctag aaacccatga
     3241 aaattttcaa gaactttcat gtattccaat aaattagtac tattatatca cattttagct
     3301 ggattgagaa aagagtaaca agctattctc cactctccaa ttcatccata tcttgttcaa
     3361 attttttctg agcccattcg ccatagctct taagaccaag attgccaata aagacccacg
     3421 gaaggtaaat gacatatgta atgaccccag cagacaggta tttaaaattc aaaggattgt
     3481 gctgataaat ttctatgttg aattgataat tctgcaacat caaaagagcc gtaatagcca
     3541 aggttaggaa aaaacaaccc aaaatcgtaa aatgaaaacg actatagtag gtcactccca
     3601 gataacgggc gcgattgaaa aagtaaaatg tccctatgat gataacgatt agcagcatat
     3661 tagaattaaa aaggcttggt gctaatactg aaatgatata agataggagc gacaaagcaa
     3721 tgcagatata gaaactttca gagcccgctt tattgaacag ttgttcttct ctttcgtcta
     3781 gtaattgata ataataaaat ctatttttca tcttcttcct cccaaaatag ttggtctagg
     3841 gttttcccta aacatctgca aatagactgg cagagcgaga gactgggatt gtattttccc
     3901 gcctctatca aaccaatagt ctggcgtgtc accccgacag cctctgccag ttgaccttgt
     3961 gttaaatcac gctctacccg agctaatttt aattttaaat ttttagccac cttcgtcctc
     4021 cttatagttt taatactcat ctactcttaa aaaatccaaa accaacacaa gctatcagct
     4081 atttattata accccattaa tttgcactcc agtattatct tcagccgaaa atcagtattt
     4141 taggttgttt gaaataaact tcctagttta ctcttttatt tttatttagt attagcttta
     4201 gtagaatctt ttaaacgagt ttttattgct tatatttctt gtttagttta gttg
//
LOCUS       DAVKGU010000119         4246 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKGU010000119 DAVKGU010000000
VERSION     DAVKGU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.121"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            248..2581
                     /locus_tag="VW077_001943"
     CDS             248..2581
                     /locus_tag="VW077_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9455892.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKEDSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2594..4057
                     /locus_tag="VW077_001944"
     CDS             2594..4057
                     /locus_tag="VW077_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9455893.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            4057..>4246
                     /locus_tag="VW077_001945"
     CDS             4057..>4246
                     /locus_tag="VW077_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEU9455894.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         1485 a          642 c          885 g         1234 t
ORIGIN      
        1 tttatttttg aaaaatagct caccagcccg actgaaagtg cttataaatg ataataagtc
       61 gcctgccgaa aacagcgaaa aatagcggtg ttatgcggag ataatctgac gcgatgcgaa
      121 agtatattgc atacttattt tcaacaattt agcagagtat ttttataagt gtgatataat
      181 agaagtataa tttgttctga tagtttattt atggagaagt agatttttag aatgcggagg
      241 gttcaatatg gttgagttta taaagtctaa gaaagaaatg agtgaggagg atattaaagc
      301 aaatttcatc actcctgcta ttgtatccaa aggatggaaa aatggtgagc atatcgctta
      361 cgaagaatac ttcactgatg gtcgaattga ggttagagga gataaggctc gtcgtaaaga
      421 aggaaaaaaa tcagactatt cactgtatta ccaatttgga actcgaattg caattgttga
      481 ggcaaaggat aataaacaca gcgttcgagc aggattacaa caagctattg aatatggaga
      541 gattttagat gttccatttg tttattcttc gaatggtgat ggctttattg aacacgaccg
      601 tatcacgaga gaagaacgtg agctggagtt agacgaattc cctactcgtg aagaattatt
      661 ttctcgtatg acgaaggaaa aaggattgac gtacgaaatt acagaagcta tctcaactcc
      721 atactataca gacgccttct caatgaaaac gccacgctat tatcagcaaa tagctatcaa
      781 ccgtactatt gaaacagttg ccagaggaca aaaacgagta atgtttgtga tggcaacagg
      841 aacggggaaa acgttcatgg cttttcaaat tattcatcgc cttcgaaaag ctggtttggc
      901 taaacgagtt ttattcttag cggatagaaa catcttagta gaccaaacga tggctgaaga
      961 ctttaggcca ttcgaaaagg taatgacgaa aattacacca aaacttttga ctgctcctga
     1021 aaaattaaat tcttttgaaa tttatctagg gctttatcag caactaactg gtgaagatgg
     1081 aactgaaaca cattatcaaa aatttgacaa agacttcttt gatttaatcg taattgatga
     1141 agcgcaccgt ggttcagcta aggaagacag taactggcgt aaggtgattg attatttcag
     1201 ttctgcgaca cagattggga tgaccgctac tcctaaagaa accaagaatg cttccaatac
     1261 ggaatacttt ggtgagccaa tctatactta tagtttaaaa cagggaatcg aggatggttt
     1321 tttggctcca tatcgtgtta tgagggttaa tttagatgtg gatgtggatg gttatcgtcc
     1381 agaaactgga aaagttgata ctaacggaca attaatagaa gataggtact acggcaggaa
     1441 agattttgat aaaaccattg tcattgatga tagaacgcaa agagttgcca agtttgtttc
     1501 tgattatatg aagcaaaaca atgcacgatt tgataaaaca attgtttttt gtgttgatat
     1561 tgaccatgcc gagcgaatgc gtgctgcact tgtaaaagag aatctagact tagtccaaga
     1621 agactatcgt tatgtcatgc aagtaactgg tgacaacgct gaaggaaaag ctcaactgga
     1681 taactttatg gatgtcaatt ctaattttcc cgctattgta acaacgtcta aattattaac
     1741 gacaggagtt aatgctaaaa catgtcgttt gattgtttta gactctaata tccaatccat
     1801 gactgaattt aaacaaatta ttggtcgtgg cacacgtctt tatcctcaaa aggggaaaga
     1861 attttttacg attattgatt ttcgaaatgt taccaatttg tttgctgacc ctgattttga
     1921 tggtgagcca gtgaaggtgc tggaaacagg tgcgaaaaca gtcagtggtt ccacgcccgg
     1981 tttcgtagat gaggaaggtg acccagtaga aaaatatatc gttacagaca agcaggttac
     2041 cattcttaat tctactgttc aagtattgga tgaaaacggg aaactgatta ccgaaagcct
     2101 gaccgactac actcggaaga atatcttagg tagctacgcc actttgaacg attttatcac
     2161 agtttggcat acggcggata aaaagaagct tatcttagac gaactttata aaaaaggagt
     2221 ttatctagat gctattcgag agtcggaggg aatatcagaa caagaaatcg atgattttga
     2281 tttactccta aaacttgcct atggtcaaaa agaattaacc aaaacggaac gtatcaataa
     2341 actcaaacaa agcggatatt tatataaata tagtgaggaa gcgcgtgctg ttttggaaat
     2401 tttactgaac aaatacatgg ataaaggtat tggagaactc gaaagcattg aaacattaaa
     2461 acttccagaa tttcagatat atggtggaac cttcaaaatc atcaatactt attttggaga
     2521 taaaaaacga tatttacaag caattaaaga attggagcaa gagctattta cagtagctta
     2581 atgaaaggaa agtatgtcaa ttacatcatt tgtaaaaaga attcaagata tcactcgaaa
     2641 cgatgctggt gttaatggtg atgctcaacg tattgagcaa atgtcttggt tattattctt
     2701 aaaaatttat gatagccgtg aaatggtttg ggaattagaa gaagacgagt atgagtcaat
     2761 tatcccagag gaattaaaat ggcgaaattg ggctcatgct caaaatgggg aacgggtatt
     2821 gacaggcgat gaattacttg attttgtcaa taacaagtta ttcaaagagt tgaaagagct
     2881 tgaaataact tcaaatatgc ctattcgaaa aacgattgtt aaatcagctt ttgaagatgc
     2941 gaacaactat atgaaaaatg gcgtcttgtt acgccaagtc atcaatgtta ttgatgaagt
     3001 tgatttcaat agccctgaag atcgtcattc gtttaatgat atttacgaaa aaattcttaa
     3061 agatattcaa aatgctggga actcaggaga attttatacg ccacgtgcag cgactgattt
     3121 tattgccgaa gttcttgacc caaaacttgg agaatcaatg gcagaccttg cttgcggaac
     3181 aggaggcttc ttgacttcga ctctgaaccg tttaagtagt caacgtaaaa ctagtgaaga
     3241 taccaaaaaa tataatacag ctgtttttgg tattgaaaag aaagcatttc ctcatctttt
     3301 agcagttaca aatctgtttc ttcacgaaat tgatgaccct aaaattgttc atggaaatac
     3361 tttggagaaa aatgttcgtg aatatacgga tgatgaaaaa tttgacatta ttatgatgaa
     3421 tccacctttt ggaggatcag aattagaaac aataaaaaat aactttccag cagaattacg
     3481 gagttctgaa acagctgatt tatttatggc tgtcattatg tatcgtttaa aagaaaatgg
     3541 tcgtgttgga gttattttac ctgatggttt tctatttggt gaaggtgtaa aaactcgctt
     3601 gaaacaaaaa ctggtagatg agttcaactt gcatacgatt attaggttgc ctcatagtgt
     3661 ctttgcaccg tatacaggaa tccatacgaa cattcttttc tttgataaaa caaagaaaac
     3721 agaagaaact tggttttatc gtttagatat gccagatggt tataaaaatt tctcgaaaac
     3781 taagccgatg aagtcagaac acttcaatcc tgttcgtgac tggtgggaaa atcgtgaaga
     3841 gattctggaa ggtaagttct acaaatctaa atcatttaca cctagtgaat tggctgagtt
     3901 gaattataat ttagaccagt gtggctttcc aaaagaggaa gaggaaatct taaatccctt
     3961 tgagttgatt cagaattatc aagcggaaag agcaacttta aatcataaga ttgataatgt
     4021 attagctgat attttgcagt tgttggagga caaataatga caccagaaca acttaaagca
     4081 agtattctcc aaagagcgat ggaagggaaa ttagtgccgc aaaatcccaa tgacgaacct
     4141 gcaagtgaat tattaaagag aattaaagct gaaaaagaaa aacttatcag tgaaggaaaa
     4201 atcaaacgag ataaaaagga aactgagata tttcgtggtg atgatg
//
LOCUS       DAVKGU010000120         4092 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKGU010000120 DAVKGU010000000
VERSION     DAVKGU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.122"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(291..686)
                     /locus_tag="VW077_001946"
     CDS             complement(291..686)
                     /locus_tag="VW077_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9455895.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(782..1036)
                     /locus_tag="VW077_001947"
     CDS             complement(782..1036)
                     /locus_tag="VW077_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9455896.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(1046..2380)
                     /locus_tag="VW077_001948"
     CDS             complement(1046..2380)
                     /locus_tag="VW077_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9455897.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEVFEAFAIEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(2380..2613)
                     /locus_tag="VW077_001949"
     CDS             complement(2380..2613)
                     /locus_tag="VW077_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9455898.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(3028..3210)
                     /locus_tag="VW077_001950"
     CDS             complement(3028..3210)
                     /locus_tag="VW077_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455899.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(3212..3991)
                     /locus_tag="VW077_001951"
     CDS             complement(3212..3991)
                     /locus_tag="VW077_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455900.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYLILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         1191 a          945 c          723 g         1233 t
ORIGIN      
        1 gttttataac aacctccttt gactaagaca caaaagaaaa gccctgccat ctacatgaca
       61 gggacgaatg tatttatccg cggtaccacc caatttcggg cagttgcccg caactctcgt
      121 ctttaaagca aaaagacact tttatttcaa tttttcatcc attagcaacc agttttgaca
      181 catttgtgga cttctcagca ccgccacttt ctgtaaaatg tgggattcaa aacacctcta
      241 atggctctat tgtagcaagt ttttctcgga aatgcaaggc tcaagaaaaa ttacttgaac
      301 ttgatgccat tttctttcaa tttcttgatg gtagctgggc cgattccttt caaggcaagg
      361 agttcttttt cagtccagtt tttgaaatct gaagcagact tgattccttc atcatagaaa
      421 gtcttggcac gatcaagtgg aagtccaccc aagtttgctg caaaatcttc tacagaatta
      481 gctacttttt gagcttgctc tttggtagct tctactgctt gttcaacttt tttagaaact
      541 gccttaccag ctgcgcttgc tgactgttct gcacgcttca cgactttttt tgtctcatct
      601 acaaccttag tcacagtact tgaaaaggca cctgcgcgac ggaggccatt tcgtaattgt
      661 tttttacgat tgagtttctt tgacatgata aaatcctttc aataaaaaat ccctgctctg
      721 acaaggattt aacataaaca ttctatcaag attttagtga aaaatcaaga atctttctcg
      781 tttaataatt tcgctcacca aacaagcctt ctggcaaatc atggaatccc ttgcctgctt
      841 tgaagttttc aaacttacca agcaagaact gataagcatc caagcgactc tcgtagcgaa
      901 tcatggcatc tttggcacgc tcgtcctggt cttcttcgta aactttttgg ctttccttgt
      961 gcgccatgtc gttaatcatg atttgggtat tgacccaagc ttcaaattcc ttcataaatt
     1021 cttgttcgta actcattttt tctccttatt ctaatccacg aaaatagtcc gtatcaatct
     1081 cacggtaggg ctggatatcc tgaacgtatt ccagtacacc ttggaattct ccgttttcat
     1141 cgtgtactgc agcataggtg atgtggacaa acttgcctcg cgactcagac ttgaaccaca
     1201 tttcatactt gtccttggtc ccctcacgaa gtcccttcat gatagccttg accttgtcca
     1261 aatacttagg cggatgacag agttcgacat tgcgcccgac ttgggacggc gtccgtttga
     1321 aaatcatctc atcagctggc gtattgtcat tgtaatactg gaaaatatct tctttattga
     1381 caaaggtaat ctccataggg agatgattga ggatgagatt ggcctgctcg actgaaagat
     1441 agccattacc aaaagcctgt tgactatggc ggtccagcac agcttccttt tccttagggg
     1501 taaaggtaat ggtaaaatgg ccttctggcg tatctataac ttgttgaact tgaccttctg
     1561 ccgtatctag ctgtacaggc tcctctgcaa tcttttcctc aataaagctc tgtcgttctg
     1621 gcacccattt ctctgacgga cggatgatgg catagccata ggcatcgctc tcctccgcaa
     1681 tctgaagcca gtcatcctga gtaaaagact caaggagaat catgaggagg atggactctt
     1741 ccttgaaaat catactttca aactctatcg caaaagcttc aaaaacttcc tttacactgc
     1801 taattgacac ttctggtagt gacttggctg tcgttagagc tgtttgaaag agttccctaa
     1861 tctgatcatc cactccccac ataactttgg gaggtgaatc gtgtccatag cgctccatga
     1921 taggaaagaa gagttcttct ttacgttggt aatggatgtc aaattgcccc acaagtccca
     1981 tctgacgcac caagcccttg cgcatctccg caagcatttc ctcgtcttcc atcaactcat
     2041 aggtatctaa caatctacga atgcgaatca aagctgcacg gagggccaaa ttttcttcct
     2101 taaagacacg aactgggtga cctggatgct cagtatctga aacttcgaca cctttgatag
     2161 catttttaaa aagattggca tggacatcac agagttccat aacatcttca aaggtgacac
     2221 ctgagtctga gttcataagc tcgtgctcca taagggaaat ctcaatggct gagacacccg
     2281 caaaggtcgc atcaaaacgc tcctgaaccg actcaggaga ggcgccatta tgcaattcta
     2341 ataaaatatc ccgtaggaca tgaatccgtt catctgccat tagtctaatc caatcacttc
     2401 gtagccattc gcttccagtg tgcgtacaat cttgtccata ggagttcctg ctagcttaga
     2461 accctgttta agtgatactt tacgaccaac tgtattgcgc attaagggat tggcaagggg
     2521 tttaaaaccc aactccacta gaatttccaa gacttctgga tgcttgtcca ccacttctgc
     2581 aacaggaatt gacacatcga tgatattgtc catgacgacc tccattgtat gttctaatac
     2641 tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gttgctcaaa acagtgtttt
     2701 gaggttgtgg atagaactga cgaagtcagt aacatatata cagcaaggtg aagctgacgt
     2761 ggtttgaaga gattttcgaa gagtataaaa tgattttact gcttatatta taccatatgg
     2821 aaagagaata aaaaactagc agtggagttc ctgctagttc tctgcgactt ggattaccat
     2881 ctagataagt ttaaagagcc aatccaatat cttaaataat atcttgtaaa acagcaattg
     2941 gactgcaaaa gaaatgatca tccaaaagaa tttcacaatc tcaataatcg gtttgataaa
     3001 aagaatagca agaagaccaa aaaaatttca ttctctctcc tctggcttaa tgagccaccg
     3061 agccgtatcc attagcttat ctgcaataaa gagttttccc agaccaacaa cgacatggtc
     3121 atctttcttt atcgttttca tcacttttac accttgcacg gtcaaaaagt aattaccata
     3181 aattttagct agagttttta tgagtcccat attactctcc ttcgatgatt tcagcctctt
     3241 ttcggattgt ttcaacatct tcttgatatt gaacttcact atagttaact agcttggaca
     3301 attctttctg caagctttca cccatcggtt tcatagttgc aaaggttaaa ccacctgaaa
     3361 tgataccacc caagatagga ataaatttcc ccattccttt ggccagccct cccttggtaa
     3421 gattcacacc aaatattctt aaaacttttt tcaaaataag ataccaaagc gtctttgtta
     3481 aagctttatt aggtactgtt ttcattacct gtttggcaat tgttatacca ccagcacgta
     3541 gcaaagcagc agttccattc acccccaaca tgacgcctag ataaagcaaa agggtatttt
     3601 gagcatcttc actcaactca tcgcgtgaag cccaaagatc ctcataacca taaatataac
     3661 ctaattcttg ggccaatttc agagagaaac cataaaattg agccacatca gctggaatgg
     3721 taattgccat agcaagccct ccaggtaatc ctgccaaaac agaagtccca ctcgctaata
     3781 aaacattatc cctaatacag gcattagcca ctcgatctaa gatttcttga gataagagag
     3841 acgttggacc ttgttccaat aaggtaggaa tgtcctgtgg acccaattct ttggaaaact
     3901 tatccactaa aaatttcgaa cgatcaacct taacaactgg tagttttacc acttgttgta
     3961 atacttgcat agctaagtct tgttcagcca tatacaaact ccttatttct tttttacacc
     4021 ttatcatatt atatttccta caaaaaaagc attttctaat tagataacat taaaaaatac
     4081 agaacccctt tt
//
LOCUS       DAVKGU010000121         3797 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKGU010000121 DAVKGU010000000
VERSION     DAVKGU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.123"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            114..1295
                     /locus_tag="VW077_001952"
     CDS             114..1295
                     /locus_tag="VW077_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9455901.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1353..2111
                     /locus_tag="VW077_001953"
     CDS             1353..2111
                     /locus_tag="VW077_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9455902.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            2324..3685
                     /gene="dnaA"
                     /locus_tag="VW077_001954"
     CDS             2324..3685
                     /gene="dnaA"
                     /locus_tag="VW077_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9455903.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1350 a          658 c          677 g         1112 t
ORIGIN      
        1 gaaaatcaca gtttcaaaag agttatccac agtttgtgta aaacttttgt gtttaagtta
       61 taattaagct agtcagttta tactttcagt aattcaaaca tatggaggca aatatgaaac
      121 atctaaaaac attttacaaa aaatggtttc aattattagt cgttatcgtc attagctttt
      181 ttagtggagc cttgggtagt ttttcaataa ctcaactaac tcaaaaaagt agtgtaaaca
      241 actctaacaa caatagtact attacacaaa ctgcctataa gaacgaaaat tcaacaacac
      301 aggctgttaa caaagtaaaa gatgctgttg tttctgttat tacttattcg gcaaacagac
      361 aaaatagcgt atttggcaat gatgatactg acacagattc tcagcgaatc tctagtgaag
      421 gatctggagt tatttataaa aagaatgata aagaagctta catcgtcacc aacaatcacg
      481 ttataaatgg cgccagcaaa gtagatattc gattgtcaga tgggactaaa gtacctggag
      541 aaattgtcgg agctgacact ttctctgata ttgctgtcgt aaaaatctct tcagaaaaag
      601 tgacaacagt agctgagttt ggtgattcta gtaagttaac tgtaggagaa actgctattg
      661 ccatcggtag cccgttaggt tctgaatatg caaatactgt cactcaaggt atcgtatcca
      721 gtctcaatag aaatgtatcc ttaaaatcgg aagatggaca agctatttct acaaaagcca
      781 tccaaactga tactgctatt aacccaggta actctggcgg cccactgatc aatattcaag
      841 ggcaggttat cggaattacc tcaagtaaaa ttgctacaaa tggaggaaca tctgtagaag
      901 gtcttggttt cgcaattcct gcaaatgatg ctatcaatat tattgaacag ttagaaaaaa
      961 acggaaaagt gacgcgtcca gctttgggaa tccagatggt taatttatct aatgtgagta
     1021 caagcgacat cagaagactc aatattccaa gtaatgttac atctggtgta gttgttcgtt
     1081 cggtacaaag taatatgcct gccaatggtc accttgaaaa atacgatgta attacaaaag
     1141 tagatgacaa agagattgct tcatcaacag acttacaaag tgctctttac aaccattcta
     1201 tcggagacac cattaagata acctactatc gtaacgggaa agaagaaact acctctatca
     1261 aacttaacaa gagttcaggt gatttagaat cttaattgac atctatgtaa agaaagcttt
     1321 acataagaga aaagatgtgt tagtgtagaa tcatggaaaa atttgaaatg atttctatca
     1381 cagatataca aaaaaatccc tatcaacccc gaaaagaatt tgatagagaa aaactagatg
     1441 aactagcaca gtctatcaaa gaaaatgggg tcattcaacc gattattgtt cgtcaatctc
     1501 ctgttattgg ttatgaaatc cttgcaggag agagacgcta tcgggcttca cttttagctg
     1561 gtctacggtc tatcccagct gttgttaaac agatttcaga ccaagagatg atggtccagt
     1621 ccattattga aaatttacag agagaaaatt taaacccaat agaagaagca cgcgcctatg
     1681 aatctctcgt agagaaagga ttcacccatg ctgaaattgc agataagatg ggcaagtctc
     1741 gtccatatat cagcaactcc attcgtttac tttccttgcc agaacagatt ctttcagaag
     1801 tagaaaatgg caaactatca caagcccatg cgcgttccct agttgggtta aataaggaac
     1861 aacaagacta tttctttcaa cggattatag aagaagatat ttctgtaagg aaattagaag
     1921 ctcttctgac agagaaaaaa caaaagaaac agcaaaaaac taatcatttc atacaaaatg
     1981 aagaaaaaca gttaagaaaa ctactcggat tagatgtaga aattaaacta tctaaaaaag
     2041 acagtggaaa aatcattatt tctttttcaa atcaagaaga atatagtaga attatcaaca
     2101 gcctgaaata aggctgttct tttatttttt tatctcacaa ggttatccac tatgtttttc
     2161 gataaaaagc ttaataaatc aataatttct tcttttatcc ccaacctgtg gataaagttt
     2221 ggtaacattg tggattattt ttcacagctt gtggaaaatt cttgctatct atggtaaaat
     2281 atctctagta ttaaactttt aaatagtaaa ggaggagaaa ggattgaaag aaaaacaatt
     2341 ttggaatcgt atattagaat ttgcacaaga aagactgact cgatccatgt atgatttcta
     2401 tgctattcaa gctgaactca tcaaggtaga ggaaaatgtt gccactatat ttctacctcg
     2461 ctctgaaatg gaaatggtct gggaaaaaca actaaaagat attattgtag tagctggttt
     2521 tgaaatttat gacgctgaaa taactcccca ctatattttc accaaacctc aagatacgac
     2581 tagctcacaa gttgaagaag ctacaaattt aactctttat aactatagtc caaagttagt
     2641 atctattcct tattcagata cgggattaaa agaaaagtat acctttgata actttattca
     2701 aggggatgga aatgtttggg ctgtgtcagc cgctttagct gtctctgaag atttggctct
     2761 gacctataac cctcttttta tctatggagg accaggcctt ggtaagactc acttattaaa
     2821 cgctattgga aatgaaattc taaaaaatat tcctaatgcg cgtgttaaat atatccctgc
     2881 cgaaagcttt attaatgact ttcttgatca cctaagactt ggggaaatgg aaaagtttaa
     2941 aaagacctat cgtagtcttg atcttttgtt aatcgatgat atccagtcac tcagcggaaa
     3001 aaaagtcgca actcaggaag aatttttcaa tacctttaac gcccttcatg acaagcaaaa
     3061 acagattgtc ctaacgagtg atcgtagtcc aaaacatcta gaagggctcg aggagaggct
     3121 tgtcacgcgt tttagttggg gattgacaca aactatcaca ccccctgact ttgaaacacg
     3181 tattgccatt ttacaaagta aaacggaaca tttaggctac aatttccaaa gtgatactct
     3241 agaataccta gctgggcaat ttgattcaaa tgttcgagat cttgagggag ccatcaacga
     3301 catcacttta attgccagag taaaaaaaat caaggatatc actattgata ttgctgcaga
     3361 agccattaga gcccgcaaac aagatgttag ccaaatgctc gtcatcccaa ttgataaaat
     3421 ccaaactgaa gttggtaact tttatggtgt tagtatcaaa gaaatgaagg gaagtagacg
     3481 ccttcaaaat attgttttgg cccgtcaagt agccatgtat ttatctagag aactaacaga
     3541 taatagtctt ccaaaaattg ggaaggaatt tgggggaaaa gatcatacca cagtcattca
     3601 tgcccatgcc aaaataaaat ctttgattga tcaagacgat aatttacgtt tagaaattga
     3661 atcaatcaaa aagaaaatca aataatttgt ggataacttt tagtttttta tcttttttat
     3721 ccacattttt taaacaagct aaaaaacttg atatgacttg tttaaaggct gttttccaca
     3781 gatttcacag actctat
//
LOCUS       DAVKGU010000122         3764 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKGU010000122 DAVKGU010000000
VERSION     DAVKGU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.124"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            158..385
                     /locus_tag="VW077_001955"
     CDS             158..385
                     /locus_tag="VW077_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455904.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            673..3570
                     /gene="nanA"
                     /locus_tag="VW077_001956"
     CDS             673..3570
                     /gene="nanA"
                     /locus_tag="VW077_001956"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9455905.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDASAPRFYNLFSVSS
                     DTKEDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGNPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1292 a          634 c          848 g          990 t
ORIGIN      
        1 gttttaaaaa taaggtataa taaatttact aacacttata aaaagtgata gaatctatct
       61 ttatgtatat ttaaagatag attgctgtaa aaatagtagt agctatgcga aataacagat
      121 agagagaagg gattgaagct tagaaaaggg gaataatatg atatttaagg cattcaagac
      181 aaaaaagcag agaaaaagac aagttgaact acttttgaca gtttttttcg acagttttct
      241 gattgattta tttcttcact tatttgggat tgtccccttt aagctggata agattctgat
      301 tgtgagcttg attatatttc ccattatttc tacaagtatt tatgcttatg aaaagctatt
      361 tgaaaaagtg ttcgataagg attgagcagg aagtatggtg taaatagcat aggctgatgt
      421 ccatcatttg cttataaaga gatattttag tttaattgca gcggtgccct ggtagataaa
      481 ctagattggc aggagtctga ttggagaaag gagaggggaa aattggcacc aatttgagat
      541 agtttgttta gttcattttt gtcatttaaa tgaactgtag taaaagaaag ttaataaaag
      601 acaaactaag tgcattttct ggagtaaatg tcttatttca gaaatcggga tatagatata
      661 gagaggatca gtatgaatcg gagtgttcaa gaacgtaagt gtcgttatag cattaggaaa
      721 ctatcggtag gagcggtttc tatgattgta ggagcagtgg tatttggaac gtctcctgtt
      781 ttagctcaag aaggggcaag tgagcaacct ctggcaaatg aaactcaact ttcgggggag
      841 agctcaaccc taactgatac agaaaagagc cagccttctt cagagactga actttctggc
      901 aataagcaag aacaagaaag gaaagataag caagaagaaa aaattccaag agattactat
      961 gcacgagatt tggaaaatgt cgaaacagtg atagaaaaag aagatgttga aaccaatgct
     1021 tcaaatggtc agagagttga tttatcaagt gaactagata aactaaagaa acttgaaaac
     1081 gcaacagttc acatggagtt taagccagat gcatcagctc cacgctttta caatctcttc
     1141 tctgtatcca gtgacaccaa ggaagatgag tacttcacta tggcagttta caataatact
     1201 gctactctag aggggcgtgg ttcggatggg aaacagtttt acaataatta caacgatgca
     1261 cccttaaaag ttaaaccagg tcagtggaat tctgtgactt tcacagttga aaaaccgaca
     1321 gcagaactac ctaaaggccg agtgcgcctc tacgtaaacg gggtattatc tcgaacaagt
     1381 ctgagatctg gcaatttcat taaagatatg ccagatgtaa cgcatgtgca aatcggagca
     1441 accaagcgtg ccaacaatac ggtttggggg tcaaatctac agattcggaa tctcactgtg
     1501 tataatcgtg ctttaacacc agaagaggta caaaaacgta gtcaactttt taaacgctca
     1561 gatttagaaa aaaaactacc tgaaggagcg gctttaacag agaaaacgga catattcgaa
     1621 agcgggcgta acggtaaccc aaataaagat ggaatcaaga gttatcgtat tccagcactt
     1681 ctcaagacag ataaaggaac tttgatcgca ggtgcagatg aacgccgtct ccattcgagt
     1741 gactggggtg atatcggtat ggtcatcaga cgtagtgaag ataatggtaa aacttggggt
     1801 gaccgagtaa ccattaccaa cttacgtgac aatccaaaag cttctgaccc atcgatcggt
     1861 tcaccagtga atatcgatat ggtgttggtt caagatcctg aaaccaaacg aatcttttct
     1921 atctatgaca tgttcccaga agggaaggga atctttggaa tgtcttcaca aaaagaagaa
     1981 gcctacaaaa aaatcgatgg aaaaacctat caaatcctct atcgtgaagg agaaaaggga
     2041 gcttatacca ttcgagaaaa tggtactgtc tatacaccag atggtaaggc gacagactat
     2101 cgcgttgttg tagatcctgt taaaccagcc tatagcgaca agggtgatct atacaagggt
     2161 gaccaattac taggcaatat ctacttcaca acaaacaaaa cttctccatt tagaattgcc
     2221 aaggatagct atctatggat gtcctacagt gatgacgacg ggaagacatg gtcagcgcct
     2281 caagatatta ctccgatggt caaagccgat tggatgaaat tcttgggtgt aggtcctgga
     2341 acaggaattg tacttcggaa tgggcctcac aagggacgga ttttgatacc ggtttatacg
     2401 actaataatg tatctcactt aaatggctcg caatcttctc gtgtcatcta ttcagatgat
     2461 catggaaaaa cttggcatgc tggagaagcg gtcaacgata accgtcaggt agacggtcaa
     2521 aagatccact cttctacgat gaacaataaa cgtgcgcaaa atacagaatc aacggtggta
     2581 caactaaaca atggagatgt taaactcttt atgcgtggtt tgactggaga tcttcaggtt
     2641 gctacaagta aagacggagg agtgacttgg gagaaggata tcaaacgtta tccacaggtt
     2701 aaagatgtct atgttcaaat gtctgctatc catacgatgc acgaaggaaa agaatacatc
     2761 atcctcagta atgcaggtgg accgaaacgt gaaaatggga tggtccactt agcacgtgtc
     2821 gaagaaaatg gtgagttgac ttggctcaaa cacaatccaa ttcaaaaagg agagtttgcc
     2881 tataattcgc tccaagaatt aggaaatggg gagtatggca tcttgtatga acatactgaa
     2941 aaaggacaaa atgcctatac cctatcattt agaaaattta attgggaatt tttgagcaaa
     3001 gatctgattt ctcctaccga agcgaaagtg aagcgaacta gagagatggg caaaggagag
     3061 atgggcaaag gagttattgg cttggagttt gactcagaag tattggtcaa caaggctcca
     3121 acccttcaat tggcaaatgg taaaacagcg actttcctaa cccagtatga tagcaaaacc
     3181 ttgttgtttg cagtagataa ggaagatatc ggacaggaaa ttattggtat agctaaagga
     3241 agcatcgaaa gtatgcataa tcttcctgta aatctagcag gtgccagagt tcctggcgga
     3301 gtaaatggta gcaaagcagc ggtgcatgaa gttccagaat ttacaggggg agttaatggt
     3361 acagagccag ctgttcatga aatcgcagag tataagggat ctgattcgct tgtaactctt
     3421 actacaaaag aagattatac ttacaaagct cctcttgctc agcaggcact tcctgaaaca
     3481 ggaaacaagg agagtgacct cctagcttca ctaggactaa cagctttctt ccttggtctg
     3541 tttacgctag ggaaaaagag agaacaataa gagaagaatt ctaaacattt gattttgtaa
     3601 aaatagaagg agatagcagg ttttcaagcc tgctatcttt ttttgatgac attcaagctg
     3661 atacgaaatc ataagaggtc tgaaactact ttcagagtag tctgttctat aaaatatagt
     3721 agattgaaat aagatgtgaa caactctatc aggaaagtca aatt
//
LOCUS       DAVKGU010000123         3671 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKGU010000123 DAVKGU010000000
VERSION     DAVKGU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.125"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            18..236
                     /gene="nrdH"
                     /locus_tag="VW077_001957"
     CDS             18..236
                     /gene="nrdH"
                     /locus_tag="VW077_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9455906.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            317..2476
                     /gene="nrdE"
                     /locus_tag="VW077_001958"
     CDS             317..2476
                     /gene="nrdE"
                     /locus_tag="VW077_001958"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9455907.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2664..3626
                     /gene="nrdF"
                     /locus_tag="VW077_001959"
     CDS             2664..3626
                     /gene="nrdF"
                     /locus_tag="VW077_001959"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9455908.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNCGLD"
BASE COUNT         1136 a          794 c          692 g         1049 t
ORIGIN      
        1 tgaagaggag ttacacaatg gtaaccgttt attctaaaaa caattgtgtc caatgtaaaa
       61 tgaccaagcg tttcttggac agtaataatg tctcttatcg tgaaatcaat cttgatgagc
      121 aacctgagta cgtcgatcaa gttaaagagc tcggttttag cgcagctcct gttatccaaa
      181 caccaactga agtcttttca ggtttccaac caggaaaact gaaacaatta gcataatctt
      241 agtacatcat ccagaagaaa ctgcttttag ggctaactta gaagcctttc ttttgtaatt
      301 agataaggga aattttatgg gattaaaaca tcttgaagac gtgacttact tccgtcttaa
      361 taacgaaatt aaccgtcctg ttaatggaca aatcatgctt cataaagata aggaagcctt
      421 ggatgctttc tttaaagaaa atgtagttcc aaacactatg gtttttgatt caatcaaaga
      481 caaaatcaat tacctcattg aacacaacta catcgaaaca gcctttatca agaaataccg
      541 tccagagttc ttggaagaat tggctcaatt tatcaaagac caaaacttcc aattcaagtc
      601 attcatggca gcttataaat tttacaatca atatgcctta aagactaatg acggtgaata
      661 ctatcttgaa aatatggaag accgtgtctt cttcaacgcc ctttatttcg ctgatgggaa
      721 tgaagctgtt gcaatcgata ttgccaatga aatcatccac caacgctacc aacctgctac
      781 tccttccttc ttgaatgctg gacgtgctcg tcgtggggag ttggtatctt gtttcctaat
      841 ccaagtgacg gatgatatga actctatcgg gcgttctatc aactcagctc ttcaactttc
      901 acgtattggt ggtggtgtgg gaattaccct cagcaacctt cgtgaagctg gtgcacctat
      961 caaaggctat gaaggagctg cttcaggtgt cgttcctgtt atgaagcttt ttgaagacag
     1021 cttctcttac tccaaccaat tggggcaacg tcaaggtgcc ggcgttgtct acctcaacgt
     1081 ctttcaccca gatatcatcg ctttcctttc aactaagaaa gaaaacgctg atgaaaaagt
     1141 acgtgtcaag actctatcac ttggtgttgt agtacctgat aaattctacg aattggctcg
     1201 taaaaatgaa gaaatgtacc tcttcagccc ttactctgta gaaaaagaat acggtgtgcc
     1261 attcaattac atcgacatta ctgaaaaata cgatgaattg gtcgcaaatc caaatattcg
     1321 caagacaaaa atcaaggcgc gggatttgga aactgaaatc tctaaattgc aacaagagtc
     1381 tggctatcct tatgtagtca acatcgatac ggctaaccgt gcaaatcctg ttgatggaaa
     1441 gattatcatg agtaacttgt gttctgagat tcttcaagtt caagaaccaa gcttgatcaa
     1501 cgatgctcaa gaattccttc aaatgggaac agatgtttca tgtaacctgg gttcaaccaa
     1561 cgtggttaac atgatgactt cacctgattt tggtcgttct atccgtgcta tggttcgtgc
     1621 ccttactttc gttacagata gttcacatat cgtagctgta ccaacgattg accatggaaa
     1681 tagccaagcc cacacctttg gtctcggtgc catgggactg cacagctacc ttgcccaaca
     1741 actcattgaa tatggttcac ctgagtctgt tgaatttaca agcatctact ttatgcttat
     1801 gaactactgg accttggtag agtcaaacaa tattgcacgt gaacgtggta ttaccttcca
     1861 caactttgaa aaatcagact atgctaacgg aagttacttt gacaagtatg taactggtga
     1921 atttgttcca acatcagacc gtgttaaaga actcttcaaa aatgttttta tccctggtgt
     1981 tgctgattgg gctgaacttc gcgacaaggt tcaagaagat ggtctttacc accaaaatcg
     2041 ccttgctgta gcgccaaatg gttctatcag ctatatcaac gacgtttctg cttctatcca
     2101 cccgattacg caacgtatcg aagaacgcca agagaagaaa attggtaaaa tctactaccc
     2161 tgctgctggc ttgtctacag aaaccattcc ttactacact tctgcctacg atatggatat
     2221 gcgtaaagtc atcgatgttt acgctgctgc gactgaacac gtggatcaag gactttcact
     2281 caccctcttc atgcgcagtg acattccaaa aggcctttac gaatggaaga gggaaaataa
     2341 acaaacgaca cgtgatttgt ccatccttcg taactatgcc tttaacaagg gaatcaagtc
     2401 tatctactac gtccgtacct ttacagatga cggtggagaa gtcggcgcca accaatgtga
     2461 aagctgtgtg atttagtttc tagcatagaa acattatttt tccctaagca gcactgataa
     2521 aatcaaatct actatgtcat aatataattt atgtaacatt ttaaaagtcg ggcttccgac
     2581 tttttgtgcg atactcctat agaattatgg taaagtagaa ataattgtga gttcgcaata
     2641 ctaaacacag aaagagattt taaatggaaa cttactacaa agccattaac tggaatgcca
     2701 tcgaagatgt catcgacaaa tcaacttggg aaaaactgac ggagcaattt tggctcgata
     2761 cacgtattcc cttatcaaac gacttggatg actggagaaa gctatctaac aaagagaaag
     2821 acttggtcgg aaaagttttt ggtggtttaa cccttcttga cactatgcaa tctgaaactg
     2881 gggttcaagc ccttcgcgca gacatccgta caccacatga ggaagctgtt ttcaataaca
     2941 tccaatttat ggaatctgtc cacgctaaat cttactcatc aatcttttct accttgaata
     3001 ctaaggctga gattgaagaa attttcgaat ggaccaatac caatccttac ctacaaaaga
     3061 aggctgagat tgtcaacgaa atctacctaa acggcagccc acttgaaaag aaagttgcca
     3121 gcgtcttcct cgaaaccttc ctcttctact ctggtttctt cactcccctc tactatctcg
     3181 gtaacaacaa actagccaac gttgcagaaa tcattaaatt gattattcgt gacgagtctg
     3241 ttcacggaac ctacattggt tacaaattcc aacttggttt caatgaattg cctgaagaag
     3301 agcaagaaaa actcaaagaa tggatgtacg acctactcta tactctttat gaaaatgaag
     3361 aaggctacac agagagtctt tatgacggtg ttggttggac tgaagaggtt aaaaccttcc
     3421 ttcgctacaa tgccaataaa gctctcatga acatgggaca agatccactt ttcccagatt
     3481 cagctgaaga tgtcaaccca atcgttatga acggtatttc aacaggaact tccaaccatg
     3541 acttcttctc tcaagtcgga aatggttacc ttcttggcga agttgaagcc atgcaagatg
     3601 atgactacaa ctgcggtttg gactaatact cttcgaaaat caaattcaaa ccacgtcagc
     3661 ttcgccttgc c
//
LOCUS       DAVKGU010000124         3569 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKGU010000124 DAVKGU010000000
VERSION     DAVKGU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.126"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..232
                     /locus_tag="VW077_001960"
     CDS             <1..232
                     /locus_tag="VW077_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9455909.1"
                     /translation="KGLLIWVNALTLSDSIILSAKIDDDTAIAHDGESWGKLVSIGFD
                     IIQTDWPLLLYQYLVEKNKKIKIKENYHVQKL"
     gene            216..1277
                     /locus_tag="VW077_001961"
     CDS             216..1277
                     /locus_tag="VW077_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9455910.1"
                     /translation="MFKNFKKIAFTSSLILLAACSNNASNTNQSTDEGSVDKSQEIVI
                     YTNSASNGRADWLQTKASEQGYNLRLVDISGGELADRLIAEKNNAVADMVIGLNKLEF
                     NRIKAEKLLVKYSPKWADEVDSSLGDSEGYYSPIVNQPLVLIGNKDAKMPSDWTDFTD
                     SSYKGKYGISKLSTGTSKNIFASIVSRYKDEKGELGISDEGWKVAKAYLKNAHVYAEG
                     EDYISSIMDNNNDLKYSMMWGSGVLQNQKEREYKFQVMSPKVGVPYVTEQVGILNTSK
                     KQALLKEFVDWFGSSELQKEWSDKFGSIPANKKALEQAKDELKQFANSVKPQELDWEF
                     IGKNIDSWIEKAQLEFVEQ"
     gene            1294..2298
                     /locus_tag="VW077_001962"
     CDS             1294..2298
                     /locus_tag="VW077_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455911.1"
                     /translation="MITFKDIQIKYNDFIAIDNLNLTINEGEFFTFLGPSGCGKSTTL
                     RALVGFLEPSQGQILVDNQDITNLDPEKREIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VSPEIIKAKVHEIAKKINISDEQLNRNISELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVSLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGFIEQVGTPY
                     DIYNQSATQFVCDFIGSINKISQEVLKKLQGENKYFKETEGYIRLERVKLEKTSPQDL
                     SITGVISDIEFNGVLIKYKVDLSNSESIHIIEKNDGLSRHIIKEKVTMYINPRDIMQF
                     "
     gene            2310..>3569
                     /locus_tag="VW077_001963"
     CDS             2310..>3569
                     /locus_tag="VW077_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9455912.1"
                     /translation="MRKSLNWKDCLIRLGVLWLIFALIIYPNIELLSGVFYKNGSFTL
                     EAFNKIIHSQRAMSSIFNSFILAISMVFTVNIVGTLIVLLTEYWDIRGSKILKLGYMS
                     SLVYGGIVLATGYKFVYGANGLVTKTLSSIIPGLAPNWFQGFGAVLFIMTFACTSNHI
                     MFLTNAVRSVDYHTIEAAKNMGASPLKVFFSVVFPTFKPTLFALTILTFLTGLSAVSA
                     PLIVGGKDFQTINPLIISFAKTTSSRDLAALLAIMLGLATILLLTLLNHVEKGGNYVS
                     VSKTKASIKKQIISSPLWNTLAHIVAYVLFAIYMLPIVLIILYSFTDSLTIKTGTLDL
                     SKFTLANYANLFTDAQSFRPYLVSVVYAILAAVIATIIAIVISRIVHKNKSKFDKFFE
                     YGALIPWILPGTLIALGLMFTYNIPHLI"
BASE COUNT         1224 a          504 c          657 g         1184 t
ORIGIN      
        1 aaaaggatta cttatttggg ttaatgcttt aaccttaagt gattcaatta ttctttcggc
       61 taagattgat gatgatacag ctattgctca tgatggcgaa tcttggggga aattggtttc
      121 aataggattt gatattattc aaacagattg gccattatta ctttatcaat atctagttga
      181 aaaaaataaa aaaataaaaa taaaggagaa ctatcatgtt caaaaacttt aaaaagattg
      241 cattcacttc ttcactaatt ttacttgctg cttgctcaaa caatgcttca aatacaaatc
      301 agtctactga tgaaggaagt gttgataaat ctcaagaaat tgtaatctat acaaattcgg
      361 cttctaatgg acgtgcagat tggttacaaa ctaaagcatc agagcaagga tacaatcttc
      421 gtttggtaga tatttcaggt ggagaactag ctgaccgttt gattgcagaa aagaataatg
      481 ctgtggctga tatggtaatt ggattaaata aattagaatt taaccgtatt aaagctgaaa
      541 aacttcttgt aaaatactca ccaaaatggg ctgatgaagt agatagctct ttgggagatt
      601 ctgaagggta ttattcacca attgtaaatc aaccattagt tttaattggt aataaagatg
      661 ctaaaatgcc atccgactgg acagatttta ctgattcatc ttataaaggt aaatatggta
      721 tttcaaaatt aagtacaggt acatctaaga acatctttgc aagtattgtt tcgagatata
      781 aagatgaaaa aggtgagtta ggaatatctg atgaaggttg gaaagttgca aaagcttacc
      841 ttaaaaatgc acacgtttat gcagaaggtg aagactatat ctcttcaatt atggataata
      901 ataatgatct aaaatatagt atgatgtggg gatctggtgt tctacaaaat caaaaagaac
      961 gtgaatataa attccaagta atgtcaccaa aagttggtgt tccttatgta actgaacaag
     1021 ttgggatttt aaatacaagt aagaagcaag cattattaaa agaatttgta gattggtttg
     1081 gctcttcaga acttcaaaaa gaatggtctg ataaatttgg ttcaattcca gctaataaaa
     1141 aagctctaga gcaagcaaaa gatgaactca aacagtttgc taatagtgtt aaaccacaag
     1201 aacttgactg ggagtttatc gggaaaaaca tcgattcttg gattgaaaaa gcacagcttg
     1261 aatttgttga acaataatag ttaggattaa gtgatgatta catttaagga tattcaaatt
     1321 aagtataatg attttattgc catcgataat ctcaatttaa caatcaatga gggagagttt
     1381 ttcacatttt taggaccaag tggttgtggg aaatcaacaa ctcttcgtgc actagttgga
     1441 tttttggagc cttcacaggg gcaaatacta gttgataatc aagatattac gaatttagat
     1501 ccggaaaaac gcgaaattgg tattgtgttt caaagttatg cattattccc aacaatgact
     1561 gtttttgata atattgcttt tggtctaaaa gttaaaaaag tttctcctga aattattaaa
     1621 gctaaagttc atgaaattgc gaaaaaaatt aatatctcag acgagcaact aaaccgaaat
     1681 atttctgaat tatcaggggg acaacaacag cgtgtagctt tagcacgtgc attagtatta
     1741 gaaccaaaaa ttctttgttt ggatgaaccg ttatcaaacc tagatgccaa actacgagtt
     1801 tcgttgagaa aagaattgaa acgattgcaa aaagaactag gaatcactac tctttatgtc
     1861 acacatgatc aagaagaagc attaacatta tcagatagga ttgctgtgtt taataatgga
     1921 tttattgagc aagttggcac gccgtatgat atttataatc aatccgcaac tcaatttgtc
     1981 tgtgatttta ttggctcaat caataaaatt agccaagaag ttcttaaaaa attacaaggc
     2041 gagaataaat actttaaaga aactgaagga tacatacgct tagagcgtgt taagttagag
     2101 aaaacttcac ctcaagattt atctataaca ggggtaatta gtgatattga gtttaatggt
     2161 gtacttatta agtataaagt agacttgtcg aatagtgaaa gtattcatat tatcgaaaag
     2221 aatgatggac tatcacgtca tataatcaaa gaaaaagtca ctatgtacat taatccgcgt
     2281 gatataatgc aattttaagg agattgtgca tgagaaagag tctaaactgg aaagattgtc
     2341 tcataagatt aggagtttta tggctaatct ttgccctgat tatttatcca aatattgaac
     2401 ttttgtctgg agtgttttat aaaaatggtt catttactct agaggcattc aataagatta
     2461 tacactctca gagagcgatg agtagtatat ttaatagctt tattttggcg atttcaatgg
     2521 tctttactgt aaatattgtt gggacattga ttgttctact cacagagtac tgggatatta
     2581 ggggttcaaa aatacttaaa ttaggttata tgagctctct agtatatgga ggaattgtat
     2641 tagcgacagg atataaattt gtttatggcg ctaatggtct tgtaactaaa acgctctcaa
     2701 gtataattcc aggtcttgcc cctaactggt ttcaaggatt tggtgcagta ttgtttatta
     2761 tgacatttgc ttgtacctca aatcacataa tgtttttaac aaatgcagtt cgtagtgttg
     2821 attatcatac aattgaggca gctaaaaata tgggtgcaag tcctttaaaa gttttcttta
     2881 gtgtggtttt tccaactttt aaacctacgt tatttgcctt aaccatctta acttttttga
     2941 caggtctttc agcagtttct gctcctttga tagtaggggg gaaagatttt caaactatta
     3001 acccattgat tatcagtttc gctaaaacta caagttcgag agatttagca gctttactag
     3061 ctatcatgtt aggtttagca actattctat tattgacact tttgaatcat gtcgaaaaag
     3121 gaggtaatta tgtatctgtt tcgaaaacca aggctagtat taagaagcag ataatttctt
     3181 ctccattatg gaatactctt gctcacattg ttgcgtatgt attgtttgca atctatatgt
     3241 taccaatagt tttgataatt ctatactcgt ttacagactc gttgacgatc aagacgggaa
     3301 ctttagatct atctaaattt acgttagcga actatgctaa tttatttaca gatgctcaat
     3361 catttcgtcc ttacctagtc agcgttgttt atgcaatttt agctgcggta attgctacaa
     3421 ttatagcaat agtaatttca cgtattgtac ataaaaacaa gagtaagttc gataaattct
     3481 ttgagtatgg tgcactgata ccatggatat tgccaggtac attgattgca ctaggattaa
     3541 tgttcactta taatatacct catttaata
//
LOCUS       DAVKGU010000125         3554 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKGU010000125 DAVKGU010000000
VERSION     DAVKGU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.127"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(92..1228)
                     /gene="lctO"
                     /locus_tag="VW077_001964"
     CDS             complement(92..1228)
                     /gene="lctO"
                     /locus_tag="VW077_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9455913.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCNVENPNTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1853..3343)
                     /gene="lysS"
                     /locus_tag="VW077_001965"
     CDS             complement(1853..3343)
                     /gene="lysS"
                     /locus_tag="VW077_001965"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9455914.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQSTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3471..>3554)
                     /locus_tag="VW077_001966"
     CDS             complement(3471..>3554)
                     /locus_tag="VW077_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9455915.1"
                     /translation="PMAQQVIPKAAFGYIASGAEDTFTSFQ"
BASE COUNT         1017 a          764 c          700 g         1073 t
ORIGIN      
        1 cttatttcat tttactatag ttttcgatag caatttattc ttccaataca cgaagaaaaa
       61 cctccacatt cagtggaggc aatctgtttt atcaatacaa ttttaagtca cgagggtcaa
      121 ctgggaaggt tgggttgtat ggattgtgac ggagcttgaa gtgtttgaca tcttcaatgg
      181 tctgagctcc agacaattgc ataactgtct tcaattccgc attcaagtgt tcaaagactt
      241 gacgcacacc gacactacca ccgagagcca agccatagat gacagggcgt ccaatagcaa
      301 ccaagtctgc tcctgatgcc aaggctttaa agacgtgttg accacgacga acaccagagt
      361 caaagacaat cggcacacgt ctatcaactg cttctgccac ttcttgaagc gagtcaaagg
      421 cagctggtcc accgtcgatt tgacgaccac cgtggttggt tacccagata ccagaagctc
      481 ctgcagcaag cgaacgttca acgtcctcac ggcattgtgg tcccttgaca tacacaggaa
      541 gaccagagta ttcagcgata aattctacat cgcgtggaga caagcgttgt ttagctgatt
      601 tgtaaacaaa gtccattgat ttaccagcac cttctggcag gtattcttca acaatcggca
      661 tgccaactgg gaagacaaaa ccattacgct tatccacttc acgattcccc cctacagtag
      721 catctgccgt caagacaatc gctttataac cttcagcctt cacacggtcc atgatgtggc
      781 ggttgatacc gtcatcctta ctaaagtaaa attgaaacca atgaggtgtc ccttgaaggg
      841 cttcagaaat ctctggaagg tcaacagtag agtaagaact ggttgtataa agagaaccaa
      901 actcatgcac accacgcgca gtcgccactt ccccctgttc atttgccaat ttatgagccg
      961 caacaggtgc cataatgatt ggagaagata gtttttcacc tgcaaattca atctctgtat
     1021 ttggattttc tacattgcaa agtgtatgag gaacgatgag cttgtggtta aaggcacgga
     1081 tattctctct taaagtgaaa gtatcttccg ccccactagc gatatagcca aatgctgctt
     1141 taggaataac ttgttgcgcc attggctcca aatcataggt attgatgaag tctacatgac
     1201 cttctgcatt gcttgttttg tatgacataa actgtcctcc tcgattaagt aagcgtttgc
     1261 tttttactat acaactataa taactctttt ttcaatcatt ttcaagagtt ttgcttaaaa
     1321 accatcttat tttttcatac aatatactag ccctactttc cttaaactct aaaccttagt
     1381 ctatattaag tgaatcgttg agattttttg ctgccaggca tttgaactac ttttttattt
     1441 gctgattcta tctactaata ctataaaaaa acaagtgaat aagacaccag aacatcctct
     1501 aataggacct atggacttat atcagtccca tatatactgt ttaagcaaga tactatccta
     1561 gttgacgcta acttcctatc ccacttatta attttttatt tatagtgaat ctgaacaaat
     1621 caattgggga atccaagtca atttctagta ctttttgaaa agtttcagcg tactatcgta
     1681 aaatgaaata aaacatgcac aaattgctta gggaatttaa accaatttct aacaatgtct
     1741 tagaaatcaa agtgtactat ttcaacttca atatactaca cttcattttc tccttaaatt
     1801 cagtctcttt ttgaatcaaa aaatcctctg gtaagagcca gaggataaga atttatttca
     1861 ttgttgggaa gagcaataca tcacggatag ttgttgtatc agtgaggagc atgcagagac
     1921 ggtcgatacc gattcccaaa ccacctgttg gtggcatacc gtattcaaga gcttcaatgt
     1981 agtcatagtc gattcctgtc gcttcatcat caccaagttc tttggcttta gcttgggctt
     2041 caaaacggct aagttggtcg attgggtcgt tcaactcagt aaaggcatta ccgtactcct
     2101 tagtcatgat aaagagctcg aaacggtcag taaagcgttg gtcttcagga tttttcttag
     2161 cgagtggaga tacagctact ggatgtccat agacaaaggt tggttggatt aaagtttctt
     2221 caacaaactc ttcaaagaag gcattgatga tgtgaccaac ctcagtgtag tgtttctcaa
     2281 ctggaacttt cttctcagca gcgatagctt tagcttcttc caaagtcatg tcttgccaga
     2341 aatcgacacc agtaatttct ctgatagcat ccaccatatg aacacgctta aatggttcgt
     2401 taatcttgat ttcagtactt tggtagttga ctgggccatc acctttgact gatttagcag
     2461 cgtgttggat aatgccttca gtcaagtcca tgatgtcttg gaagtctgca taagcttggt
     2521 aaacttcgat agaagtgaac tcagggttat gagtagcgtc cattccttcg ttacggaaga
     2581 tacggccaat ttcatagaca cgttccatac cacccacaat aaggcgtttc aagtgaagct
     2641 cagtcgcgat acgaagcacc atgtcaatgt tttgggcatt gtggtgggtg ataaatggac
     2701 gggcagcagc accaccggct tcattatgaa gaacaggtgt ttccacttca aggaatcctt
     2761 tttggtcaag gtaacgacgg atttcagaga tgatttttga acgagtgaca aagcgttcaa
     2821 agctttcacg attagaaatc aagtcaaggt aacgtttacg gtaaattgtt tcaacgtctg
     2881 tcaaaccatg gaatttctca ggaagaggac gaagagcctt agacaagtgt gtgatgtggg
     2941 ttgccttgat agagagttct cccatatccg tacgcatcac ttcaccttcg acaccaagga
     3001 agtcaccaag gtctgctttt ttgaagattt cgtagttttc ttcaccgaca gcatccttac
     3061 gaacgtagat ctgaatctgg ccttcgcggt cttgaaggtg ggcaaaaccc actttacctt
     3121 taccacgttt ggttaccaag cgtcctgcga tagtagctgt ttcgttttta tcgtgtaatt
     3181 gttctttatc gaggttggca tatttatctt ttaattcttg tgaatttgca gtacgttcaa
     3241 aacgttttcc gaaaggatcg attccttgtt cgcggagcgc agccattttt tcacggcgaa
     3301 cgatctgctg gtcatttagt tcttccatat gttctgtaga catgggatcc tcctagtttt
     3361 actcaaaatt gaatacgatg agtcattttt tgcgataccc ccattttatc ataaataagc
     3421 aagaaattca cggataatct ttaaaaacta aaaagaacct gacgctaaaa tcactggaaa
     3481 gaagtgaaag tatcttccgc cccactagcg atatagccaa atgctgcttt aggaataact
     3541 tgttgcgcca ttgg
//
LOCUS       DAVKGU010000126         3485 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKGU010000126 DAVKGU010000000
VERSION     DAVKGU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.128"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(232..525)
                     /gene="rpmA"
                     /locus_tag="VW077_001967"
     CDS             complement(232..525)
                     /gene="rpmA"
                     /locus_tag="VW077_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9455916.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(542..886)
                     /locus_tag="VW077_001968"
     CDS             complement(542..886)
                     /locus_tag="VW077_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9455917.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(902..1216)
                     /gene="rplU"
                     /locus_tag="VW077_001969"
     CDS             complement(902..1216)
                     /gene="rplU"
                     /locus_tag="VW077_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9455918.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(1252..1339)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(1398..2033)
                     /locus_tag="VW077_001970"
     CDS             complement(1398..2033)
                     /locus_tag="VW077_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9455919.1"
                     /translation="MTFCAGYVDAYTFIVRGNILVAGQAGNVVFLSVELIQNNVSDIR
                     DKLLTLIAFMMGVFLLTIYKEKLRIVKKSILSLIPLAILSIIIGFVPQTVDNIYLVPP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLSMLFASWQKKVKEKASF"
     gene            2504..2803
                     /locus_tag="VW077_001971"
     CDS             2504..2803
                     /locus_tag="VW077_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9455920.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            3022..>3485
                     /locus_tag="VW077_001972"
     CDS             3022..>3485
                     /locus_tag="VW077_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9455921.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYEAPSCP
                     ECGSQMKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQI
                     PRIINQKIAQKLIEKNSMTDIAHQLAISTSTVIRKLNDFHFKHDFSRLPEIMS"
BASE COUNT         1143 a          712 c          589 g         1041 t
ORIGIN      
        1 aagtgcaaac taggaagtta gccgcaggct gtacttgagt acggcaaggt gaaactgacg
       61 tggtttgaat ttgattttcg aagagtatca atccactata catattaaaa gtatggatat
      121 caaaacaaaa aaacaggctc tccgaaaact cggaaagcct tattttaagc tgtttctagc
      181 ttcctcacct ttacatttca aggttcggga taaaaggttc aatggacctt tttatttagc
      241 gattgggtaa acagaaactt gttttttatc gcgtccttta cgttcaaagc gtactacgcc
      301 ttcaacttta gcgaacaaag tatcgtctcc accacgacca acgtttacac ctggatagat
      361 gtgtgtacca cgttgacggt aaaggattga tccacctgtt acagtttgtc cgtcagctgc
      421 tttagctcca agacgtttcg cttgtgaatc acgtccgttt gatgtagaac ctccaccttt
      481 tttgtgggcg aaaagttgca agttgttaag agtcattttt aacataatgt tttcctccgt
      541 gttagttttc tgtgataact ctggtttgga cgaactcaga agagttctcc gataagtttg
      601 ccatacctaa gaaaaatgat tcaaagaata actgggtcat ttctctctgg tgtgaaggaa
      661 gatcttttgg tatttcaacc atcagatagc caccttcatc ttcgtttaat tctaggattg
      721 gttcatagcc tgcaaatttc tcaatagaat tgataaagtt aatggcaagc gtagaaaccg
      781 atgcacacac gacatctaag ccgtattcgc cactctcggc gtgtccagta atttccgcac
      841 tcctcagctc gccatcttcg gctctctcaa agactgcctg tatcatgtgt tctccttaaa
      901 attaagcgtt gattgcgttg atgacaactt ttgtatatgg ttgacggtga ccttgtttac
      961 ggtggctacc ttttttaggt ttgtacttgt aagtaaccac tttcttttgt tttccttgtt
     1021 tttcaacagt tccaactaca gtagctccag caacaagtgg agttccgaca acagtgtttt
     1081 caccaccaac aagaacaact tcgttaaaag taacttcttg accagcttca acgttcaatt
     1141 tttcaacgta aactgcttga ccaacttcaa ctttaacttg ttttccgcca gttttgataa
     1201 ttgcgtatgt gctcattatg cacctcctat gatttttatg ggtttccccg attttttgtg
     1261 aagactcgcc tagcatcgtg ggacgaacca cttaaacttg aataatcaag caacgatgtt
     1321 cgtgcggttg cacaggatcg tgcatagtca actcttcaag tatagcatat ctcctatttt
     1381 cttacaagta ataacaccta aaatgaagct ttttctttta cttttttctg ccaagaggca
     1441 aaaagcatgc tgaggtaaaa aacgctcatc ataataggaa caccaagaat ggtcttttca
     1501 tgatagaaaa tcgtcaaata ggctgaaaag acaacgccaa ggacaaaact actaagcagg
     1561 ctaacaaata tgaatccttc acgcaaaaaa ggagtgtgct tggttcggaa ataatctcca
     1621 aaagccagca tggtccgttt gatattccct gtcataaaag cgttattata ggcaataccc
     1681 gacacttctc caaaagcagt tgtcaccagt cccatacaga aggccaaggg cggcactaga
     1741 tagatattat ctacagtttg tggcacaaaa ccaataatta ttgataggat tgctaaagga
     1801 atcaaggaca gaatagattt tttcacaatt ctcaattttt ccttataaat cgttaataaa
     1861 aagactccca tcataaacgc tattaaggtt aaaagcttat ccctaatatc cgaaacatta
     1921 ttttgtatga gctctaccga aagaaagaca acatttccag cttgtccagc tacaaggata
     1981 ttcccgcgaa caataaaagt gtaagcatcc acatatccag cacaaaacgt caaaaaagtg
     2041 ctaacctttt agactgacgt gatatttttc ttataggtaa taacctcatt ttccctccca
     2101 ttgtattttc tcttagaaat attgtaccat tttctttcta aaaaatcgta ggctaccatt
     2161 tagattttac tattagcata aaaataataa tagacaacta tttatccaaa aatagataga
     2221 ggtaacatgt ttgcaaacaa agcatacgaa cctttagtaa aatcatttcc atgaaactag
     2281 aatagagccc ctcttagcaa aaatcattat tttaatttat ttctaatcac tccttgacat
     2341 aaataactct caccaataaa agactatgtc ttaaaaaaat ggtataataa aatcaatact
     2401 tgggcttgat ggctatgcta ctaataacaa ttaggagaga aaatcaggca cttgttaaca
     2461 acaaggatta tccccttgag atgaaaggaa ctttagaaat cttatgatga acatgcaaaa
     2521 catgatgcgt caagcacaaa aacttcaaaa acaaatggaa caaagccaag ctgaacttgc
     2581 tgctatgcaa tttgttggca aatctgctca agatcttgtc caagcaacct taactggcga
     2641 taagaaagtt gtcagcattg atttcaatcc agctgtcgtt gacccagaag accttgagac
     2701 tctttctgat atgaccgttc aagccatcaa ctctgctctt gaacaaatcg atgaaactac
     2761 taagaaaaaa ctgggtgctt tcgctgggaa attgccattc taaaaacaag gagctagaac
     2821 aatgcttgtc gataacaaag gctaagaaag gtgcaaaaat tataaaaaac gcatagtatc
     2881 aagcaaccta aaactttggg ctctataata tttgtagtgg gtaaatcccc tatagatatt
     2941 atggagccta ttttgtgtag aaaaaaagtc ccatatgacc tataatgaaa agcgacaaaa
     3001 caactcatta gaaagaatca tatggaacaa ttacatttta tcacaaaatt actagacatt
     3061 aaagacccta atatccagat tttagacatc atcaataagg atacacacaa ggaaatcatc
     3121 gccaaactgg actatgaagc tccatcttgc cctgagtgcg gaagtcaaat gaagaaatat
     3181 gactttcaaa aaccttctaa aattccttat cttgaaacaa ctggtatgcc tactagaatt
     3241 ctccttagaa agcgtcgttt caagtgctat cattgttcta aaatgatggt tgctgaaact
     3301 tctatcgtca agaagaatca tcaaatccct cgtatcatca accaaaagat tgctcaaaag
     3361 ttaattgaaa agaattctat gaccgatatt gcccatcagc tggccatctc aacttcaact
     3421 gttattcgta agctcaatga ttttcacttt aaacatgatt tttcgcgtct tcctgagatt
     3481 atgtc
//
LOCUS       DAVKGU010000127         3368 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKGU010000127 DAVKGU010000000
VERSION     DAVKGU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.129"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..900)
                     /gene="phtA"
                     /locus_tag="VW077_001973"
     CDS             complement(<1..900)
                     /gene="phtA"
                     /locus_tag="VW077_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9455922.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QYREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLS"
     gene            complement(1154..2887)
                     /gene="ptsP"
                     /locus_tag="VW077_001974"
     CDS             complement(1154..2887)
                     /gene="ptsP"
                     /locus_tag="VW077_001974"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9455923.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(2893..3156)
                     /locus_tag="VW077_001975"
     CDS             complement(2893..3156)
                     /locus_tag="VW077_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9455924.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT          888 a          728 c          634 g         1118 t
ORIGIN      
        1 actcaaaggc agtttgtaga gctgtttcaa gagactatca atgtcattac tttgacttgc
       61 ttgactgtta gtgttgctgt tgttgcttgt gttagtattt gtagttcctg gattgcttac
      121 agaaggtacc cagtttgttc ttgaagtgtt atcgctattt tgtcggcgat aggttcttga
      181 atttgacaga tttccccgac cagataggaa ggcttctgca gcagctaact cgctagctga
      241 taactcattc ttaggaatgt aatggtaatg gtcgccgtga ggaacgatat aagcatcacc
      301 agtatcctct atgatatcag aagcattaaa gatataacca tcatctgtag tatagcgtcc
      361 ttgcgaacgt gccaaggcaa cagctccatc gtttcttgga gtccctcctt cacgatactg
      421 actatgttct tgtttttgtc gattgatttc ctcttttgta cggacgttat ccgcgtgggc
      481 agcatcctta aggtaaacat agtattttcc atctaccttg ataacatatc cacccttgac
      541 ctcattaaca atatcctcat cttttagctt atagtttgga tctttcatga gtaattcttc
      601 actgaaaatc gcatcataag gaaccttacc attgtaataa tgatagtggt cgccatgtga
      661 agtgacatag ccttggtctg ttatcttgat gacgatttgc tcagcattga ttccttcacg
      721 cttgctaacc tcatcaggag tcaaattctc cgttttttgc gtcgcttgtt ttccatctat
      781 ataggaaaca cgattatttt ccttaaccgt tctagcttga tacagtccca actcgtaaga
      841 acaaacactt aaaatcaaag ctgccgcaga accaacaagg tatttcttat taattttcat
      901 cgaaactctc tttctatttg atatttctag ttgcaattca tttctaaatt gcagaaactc
      961 ctctctatta cttaactggt taatagttta ctcctaaatc tccaacttgt caagaatttt
     1021 atgaaccatt taagtatttt ttgttctgac cactactttt tcaaggttgg actttctata
     1081 aaaaagattt tcttgaaaat tctttttaaa atatcaaaaa aatccctgta actgagttgc
     1141 agggacttgt cgattaatca aaattaacgt attctttttg aagttcaaga acttcttcca
     1201 ttgttgagca ttctgtaagg gcacggtttg cgtactcttc catcttagct gtgtcgagtt
     1261 tcttcatcaa gctgcgtgta cgaagtacag atgttgctga catagagaac tcatccaagc
     1321 ccattccgac aagaagtgga acagcttgtt ggtcaccagc catctcacca cacataccag
     1381 cccatttacc ttcagcgtga gctgctttga tcacattgtt aatcaagcgt aggattgatg
     1441 ggttgtatgg ttggtaaagg tatgaaactt gttcgttcat acggtctgct gccattgtat
     1501 attggatcaa gtcgtttgta ccaattgaga agaagtcaac ttctttagca aattggtctg
     1561 caagcatagc cgctgcagga atctcaatca tgataccaac ttggatgtta tccgcaactg
     1621 caacaccttc agcaagaagg tttgcttttt cttcatcaaa gactgctttc gctgcacgga
     1681 attctttcaa gagcgcaacc attgggaaca tgatacgcaa ttgaccgtga acagacgcac
     1741 gaagaagagc acggatttgt gtgcggaaca tagcatctcc agtctcagag atagagatac
     1801 gaagagcacg gaatccaagg aatgggttca tttcgtgagg catatcgaag taaggaagtt
     1861 ccttatctcc accgatatcc attgtacgaa cgacaacagg tttaccgttc attccttcaa
     1921 gaacagcctt gtatgcttca tactgctcat cttcagttgg gaagtcttga gaatccatgt
     1981 acaagaactc tgtacggtaa agtccaacag cttctgcacc gttgttgtta acaccttcaa
     2041 cgtcttttgg agtaccaata ttggcagcca actcgaagtg tttaccgtca gcagtcactg
     2101 tttgagcatc tttcaaaagt gcccattcag ctttttgttt cgcataggct tcaccagctg
     2161 ctttaaattc tgccgcttgt tcatctgttg ggttgataat cacttctcca gtgatcccgt
     2221 taacagcaag gatgtcaccg tctttaacga tttcagtgat gttatttgta cctaatacag
     2281 cagcaatttc aagtgtacgt gccatgatag ctgagtggct tgtacgtcca ccaatgttgg
     2341 ttacaaaagc ttttacaaag tttttgtcca attgagctgt atctgaagga gtcaagtcat
     2401 gcgcaatcac aatcacttct tcattgatag aagctgggtt tggcaatttt ttaccaagaa
     2461 ggtttgccaa tacacgtttt gtcacgtcgc ggatatccgc tgcgcgttct tgcatgtatg
     2521 ggttgtcttc catgccttca aagatagtga taaacatatc tgtaacttct ttcagacctg
     2581 cttctgcatt cactttcttc gcacggatag tttccttgat ttggctgatc atttctgggt
     2641 cagcaagaac cattaagtga gcatcaaaaa cttgagctgc ttcttcaccg agcgtaccta
     2701 ctgctttctc gcgaataaca gaaagctcgt cttgtgatgc ctgtagagcg gcatcaaggc
     2761 gagcttcttc tgcgtttgta tcttcgactg taatagtctc aaatgacaaa tccggctgaa
     2821 cgagtagata tgcttttgca actgcaacac cgtcagatgc tgcgattcct ttaagcattt
     2881 ctgtcatttc ccttatgcca atccttcttt ttccattgtt tctgagattg cagcgatagc
     2941 gtcatctgca tctgcacctt cagctgagat agttacgtca gcaccttgac caacaccaag
     3001 actcataaca cccataattg atttaaggtt aactgattta cctttgtact caagagtgat
     3061 atctgaagca aatttgctag cagtttgtac caacaatgtt gctggacgtg cgtgaatacc
     3121 tgtttctgcc actacgtgga aatctttaga agccatagtt tgactctcct tttgttcttt
     3181 ctttttttga gttatatgtg ataaccctta caatttagta ttataccatc ttctaggatt
     3241 tttttcaagc attttctgga ttttccttca atttcctttc agataacttg ctgaaaatct
     3301 tctatcctag ataacaaaac acaggatata gtttcttcaa aaacaattta taggataatc
     3361 attgctat
//
LOCUS       DAVKGU010000128         3340 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKGU010000128 DAVKGU010000000
VERSION     DAVKGU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.130"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..110)
                     /locus_tag="VW077_001976"
     CDS             complement(<1..110)
                     /locus_tag="VW077_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9455925.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYG"
     gene            complement(288..899)
                     /gene="rpsD"
                     /locus_tag="VW077_001977"
     CDS             complement(288..899)
                     /gene="rpsD"
                     /locus_tag="VW077_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9455926.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1094..2146)
                     /locus_tag="VW077_001978"
     CDS             complement(1094..2146)
                     /locus_tag="VW077_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9455927.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2143..2841)
                     /locus_tag="VW077_001979"
     CDS             complement(2143..2841)
                     /locus_tag="VW077_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9455928.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(3008..>3340)
                     /locus_tag="VW077_001980"
     CDS             complement(3008..>3340)
                     /locus_tag="VW077_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9455929.1"
                     /translation="INVKETSDTANGSLSPSNSGSSVTPMNHNHAPGTTDSVPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT          939 a          745 c          640 g         1016 t
ORIGIN      
        1 ccataaatgg tattacgtga gatttggaaa acgtgtgatg cttctgttat actacctgtt
       61 cgctcacaat aagagagaac ttttttacga aaatctattg aatatgccat aaaaggatta
      121 taccacattg tgtactattt ttggttcatt ttactatatc actttggact atatcggtct
      181 agttatgtct caacgattat agctggatac taagagaaat caaaaaagca ctaagggaga
      241 acgtcccaaa gtgcttaata tcaaagccta tcttattcaa taaaatatta caacattttg
      301 ttgtagaatt caacgacaag tgcttcgttg atttctgggt tgatttcgtc gcgttctggc
      361 aagcgagtca atgaaccttc caacttttca gcgtcgaatg atacgaatgc tggacgtcca
      421 agagtagctt ctactgcttc aaggattgct ggaactttca atgatttttc acgaactgag
      481 attacttgac ctggagttac gcggtatgat gggatatcaa cgcgtttccc gtcaacaagg
      541 atgtgaccgt ggtttacgaa ttgacgagct tgacgacgag tagtcgcgag accaagacgg
      601 taaacaacgt tatccaaacg acgttccaaa agaagcataa agttgaaacc taggattccg
      661 cctttgattt ttgtagcttg tacgaacaag ttacggaatt gtttttcacc tacaccgtaa
      721 gtgaaacgaa gtttttgttt ttcagccaat tgcaaaccat attctgacaa tttagaacgg
      781 ttgtttggtc cgtgttgtcc tggtacgtag ttacgacgtg ccaattcttt acctgtacct
      841 gtaagtgaaa ggccaaggcg acgagcttgt ttccaagatg gtcctgtata acgtgacata
      901 tgaatgtcct cctgatataa ataatatttc ggtggaaata gtcacttaga aagccctgat
      961 tcgtgcagat gcccttcgcc taaacagcca aggttacttg tcataagaca cctgttgacg
     1021 agcttcatgc tttcctgctg atatttcaca caaaatccat tttaccatga atagctggat
     1081 ttgtaaaggg gttttaggct ttattttcac taccagagag attgagaacg agggtaaagg
     1141 tacttcctag accgtactgg ctgctgactg tgatttcccc acccaattga tgggccaatt
     1201 cacgcgcaat cgcaagtcct aatccatgac cacctgtctt catgttacgc gaagtttcga
     1261 cacgataaag gcgtttgaaa atattttcca aatcctctgg ggcaatgccc tgcccttcat
     1321 cggtcacact gattgaaagc tggcccttct ccagcttagt caccacttcc agcttggttc
     1381 ctggagcaga atatttaaaa gcgttatcga ccagattcac caagatacga gaaagcttag
     1441 cataatctcc ctcaatccgg gcagactctg ggattacctg caagtggaca tctcttctct
     1501 cctgctcaat caaaaactga aattcactca tgcactcaat taagagcttg tccagaaaaa
     1561 tactgtcttt actggtagtt tccacctgat ttctagctgt gtttagggtc aaaaaattca
     1621 actcctcaac cagtttattg agcctctccg tctggcgtcc aatggttgct agataatgag
     1681 cttgctccga ctccttgata atcccatcca aaatcccttc taccgtcgct tggatcgaag
     1741 tgataggggt cttaatatca tgcgacaact gagcaatcat caagcccttt tctcgttcgc
     1801 tttcttccaa ggaatcaaag cttacctgca aatcatggga catctcatta aaagtttgcc
     1861 ctaattgctg aaattctaca ggaccttgaa cctccaaatt tgaaggaaaa tccttggccg
     1921 ctacccgctt ggcatgctcc ttgagtttgc ccaacgacgt aaagactggc aataggagaa
     1981 agagactaat cccagcaccg acaaggctgg caacgatggt catcccaagc aaaaagtaaa
     2041 tctcgccttt cgcaatcagc attttttgaa cagcccaaaa aacaaccaaa atggttaaga
     2101 gggttgaaat aatatatcca accaaaatat aactttttag tttcatgttt gtcctctcgg
     2161 tttctctatc ttatatccca acccccaaac tgtcttaata gtgggagttt ggtcactact
     2221 atattttgcc agctcctgtc gaagagcatg gatatgcaca ttcaaggtat tggtgtcatc
     2281 cacgtagtct tctttccaga tcttttcata gaggtctgtc ttggagaaaa ctcgctctgg
     2341 attgctagct aaaatccaca gcaattcaaa tgatttaaca gttaaatcca gcatttcttc
     2401 tcctatttga acttcatgac tactatgatt catttttaga ttgccaaggg aaatcagctc
     2461 tgtttcgccc ccacgatgaa ggcggcgcaa aatattgtgg acacgcaaaa ccagctcacg
     2521 tgggctaaaa ggcttagcaa taaaatcatc tgctcccaag ctcaggccgt aaatcttgtc
     2581 ctgttcactg gtcttagcag taataaatag gaaaggctgc tctggtgata agtattgaac
     2641 ctcactgatt aaatcataac catccatccg aggcatcatg acatctgtga taatcaaatc
     2701 aatcggtttt ttcttgaaca gctctagcgc ttccagtcca tcatgggcta ccaagacctg
     2761 ataacctgcc tgaattaagt atctctgatg aatatctgtg atttctacct cgtcgtcaac
     2821 gagtaaaatt gtctttccca tctgtctctc ctttgataaa aacagtgcta tactgcttta
     2881 agtataacac tatttttatt gttttacgat gattttaatc tgctattgaa agaaagtcct
     2941 gattgctgat tgagttgtta aactaatctt agtcagccca ctatccatca tttatttcct
     3001 tagctgatta gttttcttct tttttgtttt tcaaacctag accaccgagt aaacctgcaa
     3061 gcgcaagccc aaggaaacca atacttgcca ttgatgtttg agtctcacca gtatttggta
     3121 gcatagcttt atcctctgac atcatcgtat cagacatctt gttagcagaa gcagccatgt
     3181 tttcacctgc catcgtgttg gtagaacttg tcatggtgtc agcaggcacg ctatctgtag
     3241 tacctggagc atgattgtga ttcatcggag tcacgctaga accagagtta gaaggtgata
     3301 atgaaccatt tgctgtgtct gatgtttctt taacatttat
//
LOCUS       DAVKGU010000129         3338 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKGU010000129 DAVKGU010000000
VERSION     DAVKGU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.131"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            22..1413
                     /locus_tag="VW077_001981"
     CDS             22..1413
                     /locus_tag="VW077_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9455930.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            1556..2473
                     /gene="adcAII"
                     /locus_tag="VW077_001982"
     CDS             1556..2473
                     /gene="adcAII"
                     /locus_tag="VW077_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9455931.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            2481..>3338
                     /gene="phtD"
                     /locus_tag="VW077_001983"
     CDS             2481..>3338
                     /gene="phtD"
                     /locus_tag="VW077_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU9455932.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLS"
BASE COUNT          989 a          611 c          715 g         1023 t
ORIGIN      
        1 cagtttattt tggagtcaaa gatgaatata tttagaacaa agaatgttag tttagataaa
       61 acagagatgc ataggcattt gaagttatgg gatttgattt tgctgggtat cggagccatg
      121 gtagggacag gcgtctttac aatcacaggt actgcagctg caacacttgc tggcccagcc
      181 ctagtgattt caatcgttat ttctgccttg tgtgtgggat tatcagccct cttttttgca
      241 gaatttgcct cgcgagtacc cgctacagga ggtgcctata gttacctcta tgctatctta
      301 ggagaattcc ctgcctggtt ggctggttgg ttaaccatga tggagttcat gacagccata
      361 tcaggcgtag cttcgggttg ggcagcttat tttaaaggct tgctctctca atacgggata
      421 gcccttcctc aggctttaaa tggtaccttt aatcctcaag cagggacatt tgttgattta
      481 ttgcctattc ttgtcttggt cttggtgact tcgcttgttt tactaaatgc caaggcagcc
      541 ttacgcttta attcgattct agttattttg aaattttccg ctttagctct ctttgtttta
      601 gtaggaattt ggaatattaa gtttgataat tggagcaatt ttgctcctta cggttttgga
      661 caaatctatg gagctagcac tggtattatg gctggtgcgt ccttgatgtt cttcggtttt
      721 ttgggatttg aatccatctc tatggctgta gatgaagtca agactcctca aaaaaatatt
      781 cctagaggga ttgtcttatc gctttctatc gtaaccattc tctatgcctt ggtgaccctt
      841 gttttgactg gtgtggttca ctatagtcat ttaaatgtcg atgatgccgt tgcctttgcc
      901 cttcgtagtg ttgggattag ttgggcagcc aactatgtgt cattagtggc tatcttgacc
      961 ttgattacag tttgtatttc gatgacctat gccctatcgc gtatgattta cagtttagca
     1021 cgtgacggct tagtgcctgc tgcctttaag gaactgacga agactagcaa gataccaaag
     1081 aatgctacta tcttgacagg tctagcttca gcagtagcag caggaatgtt cccactagcc
     1141 agtattgcag cctttttaaa tatttgtacc ttagcctact tgatcatgtt ggcttatggt
     1201 ctgattcgct tacggaaaga aaaaggaatg cccaaagctg gggaatttaa aacaccattg
     1261 gtacctctgt taccaatttt atcgatcatc atttgtttat ccttcatgtt acagtataat
     1321 atgaatacct ggattgcttt cctagttgcg ttgttagtag gaagtattat ttacttcact
     1381 tatggctata agcattctac catagaagaa taaagtgaaa gtctattgag ttgttgacac
     1441 tcagcagatt tttatatgtg aaagaaattt caaatttttt ctaaaaatat cttgacagat
     1501 taaattttca ggagtagaat atttactagt taattaaatg gttaaggagt tgttcatgaa
     1561 gaaacaaaat ttatttttag tcctgttaag tgtctttctt ttgtgcttgg gggcttgtgg
     1621 tcaaaaggaa agtcagacag gaaaggggat gaaaattgtg accagttttt atcctatcta
     1681 cgctatggtt aaggaagtat ctggtgactt gaatgatgtt cggatgattc agtcaagtag
     1741 tggtattcac tcctttgaac cttcggcaaa tgatatcgca gccatctatg atgcagatgt
     1801 ctttgtttac cattctcata cactcgaatc ttgggcagga agtctggatc caaatctaaa
     1861 aaaatccaaa gtgaaggtct tagaggcttc tgagggaatg accttggaac gtgtccctgg
     1921 actagaggat gtggaagcag gggatggagt tgatgaaaaa acgctctatg accctcacac
     1981 atggctagat cctgaaaaag ctggagaaga agcccaaatt atcgctgata aactttcaga
     2041 ggtggatagt gagcataaag agacttatca aaaaaatgcg caagccttta tcaaaaaagc
     2101 tcaggaattg actaagaaat tccaaccaaa atttgaaaaa gcgactcaga aaacatttgt
     2161 aacacaacat acagcctttt cttatctagc gaagagattt gggcttaatc aacttggtat
     2221 tgcaggtatc tctcctgaac aagaaccaag tccacgacaa ctaacagaaa ttcaggaatt
     2281 tgttaagacc tataaggtta aaacgatttt tacagaaagt aacgcttctt caaaagtagc
     2341 tgaaactctt gtcaaatcaa caggtgtggg tcttaaaact ctgaatcctt tagagtcaga
     2401 cccacaaaat gacaagacct atttagaaaa tcttgaagaa aatatgagta ttctagcaga
     2461 agaattaaag tgaggaaaga atgaaaatca ataaaaaata tctagcaggt tcagtggcag
     2521 tccttgccct aagtgtttgt tcctatgaac ttggtcgtca ccaagctggt caggttaaga
     2581 aagagtctaa tcgagtttct tatatagatg gtgatcaggc tggtcaaaag gcagaaaatt
     2641 tgacaccaga tgaagtcagt aagagagagg ggatcaacgc cgaacaaatt gttatcaaga
     2701 ttacggatca aggttatgtg acctctcatg gagaccatta tcattactat aatggcaagg
     2761 ttccttatga tgccatcatc agtgaagaac ttctcatgaa agatccgaat tatcagttga
     2821 aggattcaga cattgtcaat gaaatcaagg gtggctatgt gattaaggta gacggaaaat
     2881 actatgttta ccttaaagat gcggcccatg cggacaatat tcggacaaaa gaagagatta
     2941 aacgtcagaa gcaggaacgc agtcataatc ataactcaag agcagataat gctgttgctg
     3001 cagccagagc ccaaggacgc tatacaacgg atgatggtta tatcttcaat gcatctgata
     3061 tcatcgaaga tacgggcgat gcctatatcg ttcctcatgg caatcacttc cactatattc
     3121 ctaagagtga tttatctgcc agtgaattag ctgctgccca agcctattgg aatgggaagc
     3181 agggatctcg tccttcttca agttctagtc ataatgcaaa tccagctcaa ccaagattgt
     3241 cagagaacca caatctgact gtcactccaa cttatcatca aaatcaaggg gaaaacattt
     3301 caagcctttt acgtgaattg tatgctaaac ccttatca
//
LOCUS       DAVKGU010000130         3261 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKGU010000130 DAVKGU010000000
VERSION     DAVKGU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.132"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            1..539
                     /locus_tag="VW077_001984"
                     /pseudo
     CDS             1..539
                     /locus_tag="VW077_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(773..1069)
                     /locus_tag="VW077_001985"
     CDS             complement(773..1069)
                     /locus_tag="VW077_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455933.1"
                     /translation="MLQSPYAIDTINLKKDFLEKPIDIEKFKAFLEKEEIPLAIAWQG
                     DSLHFYTKDRSILDNHLDHLLEKMVNDPEKLSDFSMDKSLDDAIDEAKSQITFK"
     gene            complement(1082..1225)
                     /locus_tag="VW077_001986"
     CDS             complement(1082..1225)
                     /locus_tag="VW077_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455934.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKGLLKILEWSRRQALEQDSAYK
                     IGV"
     gene            complement(1227..1514)
                     /locus_tag="VW077_001987"
     CDS             complement(1227..1514)
                     /locus_tag="VW077_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455935.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIVDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEV"
     gene            complement(1797..2633)
                     /locus_tag="VW077_001988"
     CDS             complement(1797..2633)
                     /locus_tag="VW077_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEU9455936.1"
                     /translation="MFSNANSFKAKIKNISKDKGIPAQQVQQHYLIEQVLKLISTSSY
                     RDSFIVKGGYLIGQMIGLDKRTTMDLDVTLKGTEMSRENLIHIFEEILCSKTDGFSFS
                     VDKLEPIRQDDEYGGFSLKLNATFDTLKEVVFIDITTGDKITPREITYSMTSIFTNES
                     IKIWTYNLETVLAEKLETIISRGLASTRPRDRYDLFTLYKLRKEEINLEVLKNALENT
                     AEKRKSKDTIYNWEEQVRGIEISDYQKELWIRYQRQFKYAKDISFDNSVQVIREIMQQ
                     IF"
     gene            complement(2633..3223)
                     /locus_tag="VW077_001989"
     CDS             complement(2633..3223)
                     /locus_tag="VW077_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEU9455937.1"
                     /translation="MVDKREKLMNSFNQYGFLTFKQVIDENLHYKTLLKMVAEGKIDA
                     EEKGLYRLPDIYLDEWFVLQYRFPKGIFSLETALWLHGLSLTIPFNMTMSFPYGTNTK
                     NIKEADICPIILRSHYSEGIIEIERLPGQFIKVYEVERVLVECLRPVHQVDLQIIAPA
                     FKKYFQQNKIHLHKLFYYAQLFKVTDKLQSYTEVLS"
BASE COUNT         1047 a          603 c          519 g         1092 t
ORIGIN      
        1 gttgaggaag agaaagcctt tcttgcccgt catttgaagg ctacagaggc aggagaattt
       61 gttacaattg atgccttatt tcaggcttat aaaaaggagt taggtcgttc ctacacacgt
      121 gatgccttct atcaactgtt gaagtgccat ggttggcgaa atattacgcc acgtccagaa
      181 catcctaaga aagcagacgc tcaaaccatt gtcgcgtcta aaaataaaat ctcaattcaa
      241 gaagacaaga aagcgcttta aaatcagtag acgttttcgt aaggttcgct tgatgtacca
      301 agatgaggct ggtttcagta gaatcagtaa actgggatct tgttggtctc caataggagt
      361 aggttcacat gtccatagtc actatatacg agaatttcgc tattgttatg gagctgttga
      421 tgcccatatt ggaagatgtc atgaatcaac ttcaagatgt catacaagga ctggagaagg
      481 aggtgataaa gtccatcgtt aatcggagat ggactagaat gctttttgaa aacagatgag
      541 tattagaaat aaatgaaaag tcaatatcaa aacaatcaga atcttgatga tgctaaattc
      601 aaaaaaacta cataataatt ttaataaggg taagtcgtac caagaaaccc caccaagata
      661 aaaacttagt ggggtttggc ggggaatata attattctcc caattattat cgcctttata
      721 ttatctcaac tctcattaaa caagagttcc gcaatcaaat tgcgatactc tattatttaa
      781 aggtaatttg ggatttagcc tcatcaattg catcgtctaa tgacttatcc atggaaaaat
      841 cagataattt ctcaggatca ttcaccattt tttctaacag atggtctaaa tgattgtcta
      901 gaatcgaacg gtctttcgtg tagaaatgca gagaatcccc ttgccaagcg atggctaaag
      961 gaatctcttc tttttctaaa aaagctttaa atttctctat atcaattggt ttttctaaaa
     1021 aatctttttt cagattaatc gtatcaatcg catagggaga ttgtagcaat tcttctaatt
     1081 tctacacccc tatcttatag gcggaatcct gttctaaagc ctgacgtcta gaccattcta
     1141 gtatctttaa aagacctttt acagtaaata aaagactacg ctcagcatat tgaactgcca
     1201 ttcgttcctg ttgttcagag gacatctcag acctccttct taacaaatag cagctttcct
     1261 tctttataac gataagctat caatttctga cgttgcttaa tcgacttgac aacctgcagt
     1321 agatctctcg aataaggttc tcgaagagta acttctactg ctttcccatc aacgatacca
     1381 acacgtttaa attcaatgta gtcttttttg agatgtccta agcccatacc aaaaggaaag
     1441 tgatgaatca attggatggt acaaccacct ttatttccca tttgtttcaa atcatacaag
     1501 tttactacct tcatgattaa ctcctttatc tagtttgtcg catcgtttaa gtctggtaac
     1561 gataaactcc gtgtctgtta gaaaattctc acacacgtct tgtgccagtt gcccttcaca
     1621 gggaaatact ctcagtccct acttacacag gcacgctaat caagacggag tggattcaat
     1681 tttcaaagaa caggtagctt tattatagat aagagtagtt gaaattttta tcacattttt
     1741 ggggttgttg gaataaaaaa agcaactcac tgctttacga atgagttgct tttgtactaa
     1801 aatatttgtt gcataatttc cctaatgacc tgaacagagt tatcaaatga gatatcttta
     1861 gcatacttaa attggcgttg ataacgaatc catagctctt tctgataatc agaaatttca
     1921 attcctctca cttgttcttc ccaattataa atagtatctt tacttttacg tttttctgcc
     1981 gtattctcaa gtgcattttt taatacttct agattaatct cttcttttct aagtttatac
     2041 aaggtgaaaa gatcatatcg atcacgtggg cgtgtcgaag ctaatcccct actgataatc
     2101 gtttctaact tttcagcaag tacagtctct agattatatg tccatatctt gatactttca
     2161 ttagtaaaga tagaagtcat tgaataagta atttctcttg gtgtaatctt atcaccagta
     2221 gtaatatcaa taaaaacaac ctcctttaat gtatcaaaag ttgcatttaa ttttaatgaa
     2281 aatcctccat attcatcatc ttggcgaata ggttctagct tatctactga aaatgaaaat
     2341 ccatcagttt tagaacaaag aatctcttca aagatatgaa tcaaattttc tctgctcatt
     2401 tcggttccct tcagagtgac atctaaatcc attgtggttc gcttatctag tccaatcatt
     2461 tgacctatta gataccctcc ttttacaatg aaggaatctc tataagaact agtagaaatc
     2521 agctttaata cttgctcaat taaataatgt tgttgtactt gttgagctgg aattccctta
     2581 tcttttgaaa tgtttttgat ttttgcttta aagctattcg catttgaaaa cattaagata
     2641 gtacctccgt ataagattgt aacttatcag ttactttaaa tagctgggca taataaaata
     2701 atttgtgtaa atgaatttta ttttgttgaa aatatttttt aaacgctggt gcaataattt
     2761 gaagatccac ctgatgaact ggtcttagac attcaaccaa aactcgttca acttcataaa
     2821 ctttaataaa ttgaccagga aggcgctcaa tttcaataat tccttcacta tagtgagagc
     2881 gtaaaataat aggacatata tctgcttcct taatgttttt ggtattcgta ccataaggaa
     2941 aactcatcgt catattaaaa gggatagtca aagataaacc atgtaaccaa agtgctgtct
     3001 ccaaagaaaa gattcccttt ggaaatcgat actgaaggac aaaccactca tctaaataaa
     3061 tatcaggtaa gcgatataac cctttttctt cagcatcgat ttttccttct gcaaccattt
     3121 ttaataaggt tttgtagtgt aaattttcat ctattacttg tttaaaagtt aaaaaaccat
     3181 actgattgaa agagttcatc agtttctctc ttttatcaac catatcattc tcctttctta
     3241 gaaataaaat gctaacactt a
//
LOCUS       DAVKGU010000131         3159 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKGU010000131 DAVKGU010000000
VERSION     DAVKGU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.133"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..119)
                     /locus_tag="VW077_001990"
     CDS             complement(<1..119)
                     /locus_tag="VW077_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9455938.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(223..372)
                     /gene="blpO"
                     /locus_tag="VW077_001991"
     CDS             complement(223..372)
                     /gene="blpO"
                     /locus_tag="VW077_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9455939.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(616..819)
                     /gene="blpN"
                     /locus_tag="VW077_001992"
     CDS             complement(616..819)
                     /gene="blpN"
                     /locus_tag="VW077_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU9455940.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(835..1089)
                     /gene="blpM"
                     /locus_tag="VW077_001993"
     CDS             complement(835..1089)
                     /gene="blpM"
                     /locus_tag="VW077_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU9455941.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            complement(1239..2043)
                     /locus_tag="VW077_001994"
                     /pseudo
     CDS             complement(1239..2043)
                     /locus_tag="VW077_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(2241..2645)
                     /locus_tag="VW077_001995"
     CDS             complement(2241..2645)
                     /locus_tag="VW077_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9455942.1"
                     /translation="MKTFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQLIAYIGAVLCLIIMLLLMWGLKYYKK"
     gene            complement(2642..2806)
                     /locus_tag="VW077_001996"
     CDS             complement(2642..2806)
                     /locus_tag="VW077_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455943.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
BASE COUNT         1050 a          644 c          456 g         1009 t
ORIGIN      
        1 tagagagtac tatccgtatc ctttttggaa gattttgaaa atatttttct aattaagtca
       61 tccatataag gaccaaatat accaactact aaaccaataa taaaactttt aaaatccata
      121 attaccacca acatgttgct acataggcta cacctccaag tatagctcca cctgcagcac
      181 cagttacacc tattcctata gcaaatggtc ccaatagaaa tgtcaaaccg ttgttgcaca
      241 cccatcaatt gcgccatatg caacccctgc tgcacaacta atttttcttc cccaatcaat
      301 atctccacct tcaacgcaag caagcatttc attatccata acagaaaatt gtgacatcat
      361 ttttgtattc atatagtatg tcacttttca gttacggaac aagtttaata taaaaattat
      421 caaaaaaaca taggcaataa agagaaaaat taatttatcg tagattagaa ataatatgac
      481 aaaacaattc aatgatgtta attcaatagt cttttgtttt ttatcagaga tacttatgga
      541 tagataaata agataggttt gaaaagcgaa gagaataata aagaatatag ccttcataaa
      601 atttagcttt catttttatg atgtagcggt ataggctaaa tatccacaaa ccactgctcc
      661 tccaattcct cctattgcag cgccccatgg tcctagaagt ctcccatatt tcactccacc
      721 cgctgcacaa cctaaagcag caactacagc tgctcctccg gaattacctc cataaacctc
      781 actcagcatt gtttcattta tattacaata agtattcata caagtctcct tttattaaaa
      841 tccacccgtt gcccctgtta ctcctgccca aagatccaca ccaaatttag ctcctatgta
      901 tccacatgct cccataaatg gtgctccaac accactcgca gcacaaatag ctgttcctag
      961 cccccagcca ccaaaagcag caccaccacc ttctaagaca ttagtttgcc aattattctt
     1021 gcctccttca atactagata acatagttat atccatttca tgaaattgtt ccataatttt
     1081 tgtatccatg acaaatactc ttttttattt ttaatttttg tcttgttgta actttgacaa
     1141 gtttagtata tcatcgtttt ttaaaatttt tcatccagat cttgaatagt catcgaaacg
     1201 tcttgaattg caaaaattac attagacttc ctgcaaaact agaatcctag ttcatgattg
     1261 ataataccag caatcaaatt tattcgtaat ccgaagcgtt tacgatgatt tcgataggtt
     1321 gttgaaatca ttttaaacgt ttttactttg gcaaagatgt tctcaacctt gcttctatcc
     1381 ttagatagcg catggttaca ggctttatct tcaactgtta gtggcttgag tttgctggat
     1441 ttacgtggag tttgtacttg aggatatatc ttcatgagtc cttaataacc actgtcagcc
     1501 aagattttac cagcttgtcc gatatttctg cgactcattt tgaacaactt catatcatga
     1561 ccatagttca cagcgatatc caaagaaata attctccctt gacttgtgac aatcgcctga
     1621 gccttcatag cgtgaaattt ctttttacca gaatcattcg ctaattcttt ttttagggcg
     1681 attgatttgt acttccgtcg catcaatcat taccgtgtcc tcagaactga gaagagttct
     1741 tgaaatcgta acaccacttt gaacaagagt tacttcaacc caccggctcc gacggataaa
     1801 gttgctttcg tggataccaa aatcagccgc aatttgttca taagttcgat attctcgcac
     1861 atattgaaga gtggccataa gaaggtcttc taggcttaat ttaggttttc gtccaccttt
     1921 tgcgtgttta agttgataag ctgtttttaa tacagctaac atctcttcaa aagtggtacg
     1981 ctgaacacca acaaggagct taaatcgtgc atcagttagt tgtttacttg cttcataatt
     2041 catagaacta tagtaaaatg aaataagaac aggataaatc gatcaggaca gtcaaatcga
     2101 tttctaacaa tgttttagaa gtagaggcgt actattctag tttcaatata ctataccata
     2161 ttttgtttcg cagggaatct attataaaag ggtaagtatt gcaaaaacac ttaccctttt
     2221 cttttatact tcattaagct ctacttttta taatacttca agccccacat gagcagaagc
     2281 atgatgatta agcagagaac agcgccaata taagcgatta gttgttgata agattctcct
     2341 gctgtgatac ctctatacaa acaaataata gacataaaac ctgtcaagcc gataaacata
     2401 agttgattgg ttctaggact aaccaaatca tcatcttcaa actctcttat cctcatttcc
     2461 ctagtgagat aaacagtaac caaaatagaa gccaagttaa taactactaa aagaaattgg
     2521 aaaactacgg aaaaatttaa aaactgacga gatagaaata gataagtaga aacaagcaag
     2581 ggcaactgac ctaagaacaa tctcgcaagg aagatgttcc gttttttagc aagaaaagtt
     2641 ttcatttctt ttctcctttc tttttattga tagcaaaata gatcataact gcaatcacat
     2701 aggctatggt ataaaatagc tgataccaag cactctccct aagcggatat agaaagatgg
     2761 acatgattag atacagaacg aaaataatca gtattttttt cttcataaga tttcctccta
     2821 aatgtgcgat ttatcttagt tgagcaagaa catttacact gctagtatag cacttatttt
     2881 gaccttggat cactcaaatc ataaatggtc atcaaaacct cttgaattgt aaaaattaaa
     2941 aaagcaagca tgaaaaacat actttcctct ttatattgta ttgataccaa cttgtttgca
     3001 gacttttcat cctgctatca catatcattt tgacaggcga aacaaaatta aagaaactcc
     3061 cctgtaaatt aagctagcaa atacagggga gaaatttatt ttttagagag tactatccgt
     3121 atcctttttg gaagattttg aaaatatttt tctaattaa
//
LOCUS       DAVKGU010000132         2946 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKGU010000132 DAVKGU010000000
VERSION     DAVKGU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.134"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            62..764
                     /locus_tag="VW077_001997"
                     /pseudo
     CDS             62..764
                     /locus_tag="VW077_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(911..1756)
                     /locus_tag="VW077_001998"
     CDS             complement(911..1756)
                     /locus_tag="VW077_001998"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9455944.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            2012..2881
                     /locus_tag="VW077_001999"
     CDS             2012..2881
                     /locus_tag="VW077_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9455945.1"
                     /translation="MLYVGIDVAKNKHDVTALNVQGKTVLKPLTFSNNKAGFELLDLS
                     FRQLNQDCLIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNPQKRLEARFDKLIEIKRLTASKIQDILSIAPR
                     SIGTTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILA
                     EIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACARF
                     SPAFKAYLKTKLE"
BASE COUNT          954 a          614 c          580 g          798 t
ORIGIN      
        1 gttgcagata gagctgacgt ggtttgaaga gattttcgaa gagtataaaa aggtgctagg
       61 catgttgatt tttcctttgt taaatgattt gtcaagaaaa atcatccata ttgacatgga
      121 tgcctttttt gctgcagtgg aaatcaggga taatcctaaa ctcagaggaa aacctgtcat
      181 tattggaagc gaccctcggc aaacaggtgg acggggagtc gtttctacct gtagttatga
      241 ggcaagagct tttggtgtcc attctgccat gagttccaag gaagcttatg aacgttgtcc
      301 ccaggctgtc tttatctcag ggaattatga gaaatacaag tctgtgggac tccagattcg
      361 agctattttt aagcgctata cagattggta gactaggcta tgatctgtat cgaaaggctc
      421 gtggcattca caactctcca gtcaaatcca atcgcatccg taaatcaatc ggcaaggaga
      481 aaacctacgg gaagattctc cgtgctgagg aagatatcaa aaaagagctg actcttctat
      541 cagaaaaagt cgctctcaat ctacatcaac aagaaaaagc tggaaaaatt gtcattttga
      601 aaatccgcta cgaggacttt tcaactctta ccaaacgaaa aagtcttgct caaaaaacac
      661 aagatgctag tcagataagc caaatagccc tgcaactcta tgaagaatta agcgagaaag
      721 aaagaggtgt ccgcctattg gggattaccc tgactggatt ttaaagctca atgaaaatca
      781 aagagcaaac taggaagcta gccgcaggct gctcaaaaca ctgttttgag gttgcagata
      841 aagctgacgc ggtttgaaga gattttctaa gagtataaaa ccttgaagag ttgccccttc
      901 aaggtttttc ttatacaaat aaacggacaa aactgtaaag tagcaaaaca ctaccaagca
      961 cgatacggta tttaccaaaa agggtgaagt cgtgtttttt cacatagctg gtcaagaagc
     1021 gaatagccac catgctgacc gcaaaagcta ctcccatcgc gaccaagagc aaaaacaatt
     1081 gcccaaagct caagagttct ccggctttca caaatttgaa aatctttaag gcactagctc
     1141 caaacataac aggaatccca agatagaagg taaattctgt cacaactgaa cgactggttc
     1201 catttaacaa accaccgaca atcgttgcac ctgaacggct agtccctggt aaaagagcaa
     1261 gaacttggaa gagtccgata tagaaagcgg tcgtataagg aagcttgtcc aactctgtta
     1321 cacttggctc gatagcacgc gctttattgc gcttttccaa atagatgaag gcaaccccgt
     1381 agataatcaa catgagagca actgaaacca tgttatggaa gtgggtatca aaccaatcat
     1441 caaatttaaa gacaccaagt aaaggcaaag tggcaatcaa gaccttcaac catagtctcc
     1501 aagtcttacg aacttcctgt ttgtccttag tcggtttaaa aggattgagc ttgttaaaat
     1561 aaatcaccat aactgctaaa atagcaccaa gctgaattac gacattaaac atggacataa
     1621 aggcttcatt ttgattttgg tattggataa actcctctgc taaaatcaag tgacctgtac
     1681 tggaaatcgg caaccattcc gtaattcctt caacaatccc gaagaagata gattttaaaa
     1741 tttcaataag atacatagat ttctccttat attatattta acgcagttag ctactgccaa
     1801 agcccaagtg gtatacttgg aataagccac tgtggattag ttcatttcct ccaatcgctt
     1861 gacgatttcc gaaagggtct aaccacagct ttttgtttca attggtaatg agtttgataa
     1921 cgcatagacg ttttatctcg tctaaggtaa caaggcaaaa agttcgtgga taaccaagta
     1981 gacttgaaat actatatctg gaggtactcc catgctttat gtgggcattg atgtcgctaa
     2041 aaataaacac gatgttacag ccttgaatgt tcaaggaaaa actgttctta aaccactcac
     2101 tttttcaaat aataaagctg gttttgaact cttagatctg tcttttcgac agctcaacca
     2161 agactgtctc atcgctctta aacttctttc tgaccccaat cgtgaacaat ttcaacacga
     2221 taatcggcaa gtagaactaa aaatactggc tagacatatt catcgtctca agaaaaaaca
     2281 gtctgattgg aaagtacaat acactcgttg tcttgatatc atctttcctg agttggataa
     2341 aatcgttgga aagcattcag aatataccta ccaactcttg acacgctacc ctaatcctca
     2401 gaaaaggctt gaggcaagat ttgataagct gatagaaatt aagcgattga ccgcttctaa
     2461 aattcaggat atcctctcaa tcgcacctcg ttctatcgga acaacatctc ctgctcgtga
     2521 attcgaaatc atcgaaatca tcaaacatta caagaggctc attgacaagg cggaaacatg
     2581 tgtcaatgac ttgatggctg agttcaactc ggtcatcacg acggttactg ggattggggg
     2641 tcgtttaggg gcggtcattt tagccgagat tcgaaatatt catgcctttg ataatcctgc
     2701 tcaattacaa gctttcgctg gactggattc ttctatttat cagtcaggtc agattgattt
     2761 agctggaaga atgatcaaac ggggttcccc tcatctgcgg tgggcactca tacaagctgc
     2821 caaagcatgc gctcgctttt cacctgcttt taaggcctat cttaagacta agttagaata
     2881 aggaaaacat tacaatgtag ccatcatcca ccttgcaaaa aaacttatcc gaaccctgtt
     2941 ttatat
//
LOCUS       DAVKGU010000133         2941 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKGU010000133 DAVKGU010000000
VERSION     DAVKGU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.135"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..154
                     /locus_tag="VW077_002000"
     CDS             <1..154
                     /locus_tag="VW077_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HEU9455946.1"
                     /translation="KKILLPLPPLALQNEFADFVAQVDKSQLAIQKSLEELETLKKSL
                     MQEYFG"
     gene            214..450
                     /locus_tag="VW077_002001"
     CDS             214..450
                     /locus_tag="VW077_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9455947.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            447..860
                     /locus_tag="VW077_002002"
     CDS             447..860
                     /locus_tag="VW077_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9455948.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            978..1943
                     /locus_tag="VW077_002003"
     CDS             978..1943
                     /locus_tag="VW077_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9455949.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            1977..>2941
                     /locus_tag="VW077_002004"
     CDS             1977..>2941
                     /locus_tag="VW077_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9455950.1"
                     /translation="MKKVKLGEVATFINGYAFKPQDWSSEGREIIRIQNLTKTSTEIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNT
                     KNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLR
                     INSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITK"
BASE COUNT         1076 a          425 c          569 g          871 t
ORIGIN      
        1 aaaaaaaata cttctccccc tccccccact agccctccaa aatgagttcg cagactttgt
       61 agcccaggtc gacaaatcac aattggcaat ccaaaaatct ctggaagaac ttgaaacttt
      121 gaagaaatct ctgatgcagg agtattttgg ctgatattct gccattgtaa ttacggtaat
      181 gatttgttat aatacttcaa aggaggaaat cagatggtag taaaaacaag aaaacaagga
      241 aattcaatca ccattacgat tccaagtgaa tttaatattc caagtggtgt taaatacgaa
      301 gcgaaattgt taccaagtgg tgagattatc tttactcctg aagaattggg gcagcaggtt
      361 tcttatgtat ctgatgatgc ctttgactta aatttagata aaatatttga cgaatacgac
      421 gatgttttca aagctttggt ggaaaaatga caatctatct gacagaaaag caaattgaaa
      481 aaataaatgc tttagcaatt caacggtatt ctccaaatga gaaaattcaa acagttagtt
      541 cttctgcctt aaatatgatt gtgaacttac cagaacaatt tgtctttggg aagtctcttt
      601 atccaacaat ttttgataaa gcaacgatac tatttgtcca attgataaag aagcatgttt
      661 ttgctaatgc taataaaaga actgctttct tcgttttggt caaattttta caattaaacg
      721 gctatcgttt ttctgtaacg gtagaagaag cagtaaaaat gtgtgtaacc atcgcagtag
      781 aagctttaac tgatgaaaaa atgacaagct actccaaatg ggtttctgaa cattctgtta
      841 gagaaaaggt caaaaagtaa cctagtatgc tggatttgaa tgagcacaag aaaataaatg
      901 aacagacaat attagaattc tgtaatgcag aaactgatat tgtctctttt tattgatgaa
      961 taagaaagtg agaaattatg gaatcaaaag ttacaattat catgcaagaa atgttacctc
     1021 ttttaaataa tgaacaatta ctagcgttga gagagagttt agaacatcat ctagtagacg
     1081 gaaaaaagca gcagaagtat tcgaataata acctgttgca actatttatt accgccaagc
     1141 aggtagaggg ctgtagctca aaaacaattc gttattacca gaggacgatt gaaaacttgt
     1201 ttaatgctat taaagagtct gtgacacaac tcacaacaga tgatttaagg agttatttag
     1261 caaattacca gtctgaaaag gattgtagta aggcaaattt agacaatatt aggcgtatat
     1321 tgtcttcttt ttttgcttgg cttgagcaag aggaatatat cattaaaaat cccattcgac
     1381 ggataaagaa aattaagact gagcaaaatg tgaaggaaac ttatactgat gaacatttgg
     1441 aaattatgcg tgataactgt gaaaatttga gagatttggc aataatagac ctactagcat
     1501 cgacaggtat gcgtgtaggg gagcttgtac agttgaatcg ttcagatatt gattttgaaa
     1561 acagagagtg tgttgtcttt ggtaaaggaa agaaggagag accagtatat tttgacgctc
     1621 ggacgaaaat tcatttaaga aattatctta acgacagaaa agatagtcac cctgctcttt
     1681 ttgtaacgtt agttggaaaa gtccagaggc ttggaattgc tggtgtagag attcgcttaa
     1741 gaaagttagg agacaaactc ggtatacaaa aggttcaccc acataagttc agaagaactt
     1801 tagcgactaa ggcaattgat aaaggtatgc ctatcgaaca agtccaaaaa ctgctaggtc
     1861 atagcaagat tgacacaacc ctggcctatg ccatggtcaa tcaaaataat gtcaagcatt
     1921 cacaccaaaa attcatctct taaaagcaaa tcccgatatt ccgaggaggt ggttgcatga
     1981 agaaagtgaa attaggggaa gtggctactt ttatcaatgg ctatgctttt aaacctcaag
     2041 attggtcatc tgaagggaga gagattatca gaatacagaa tctgactaaa acttcaacag
     2101 agataaatta ttattcgggt acgattgata aaaaatacat tgttgaagct ggtgatatcc
     2161 ttatttcgtg gtcgggtaca ttaggggttt ttcagtggtg tggaagatcc gcagtattaa
     2221 atcagcacat ttttaaagtg gtttttgata agatagatat agataaatcg tattttaaat
     2281 atgttgttga aaaaggttta caagatgctg taaaacatac tcatggttcc acaatgaaac
     2341 atttaactaa aaaatatttt gataatatta tagttccata tacaaatttg ggagagcaac
     2401 aaagaattgc aagtgagctg gatttgttaa gcaagctgat tttaaggaga caagaacaac
     2461 ttgaagagct taatctactt gtaaaatccc gatttaacga gatgtttggg gaaaataaaa
     2521 tatttgaaag cattgataac ttatttgata ttatagatgg tgataggggc aaaaattatc
     2581 ctaaatcaga tgagttgttt agtgaggagt actgtttatt tttaaataca aagaatgtta
     2641 ctaaaaacgg attttcattc gatacaaagc aatttatcac taaaacaaag gataaattac
     2701 ttcgaaaagg caaacttgag cgttatgata tagtcttgac aacaagaggt actgttggaa
     2761 atgtagcgta ctacgatgaa ttaataaaat ataaacattt acgtataaat tcaggtatgg
     2821 taatattacg tcccaagaca ccaaatctaa atcagaaatt tattatccat gttttaagga
     2881 ataataatta tagtcgagtg atatcaggaa gtgctcagcc tcagttacca attacaaaat
     2941 t
//
LOCUS       DAVKGU010000134         2924 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKGU010000134 DAVKGU010000000
VERSION     DAVKGU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.136"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            103..333
                     /locus_tag="VW077_002005"
     CDS             103..333
                     /locus_tag="VW077_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9455951.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            562..2820
                     /locus_tag="VW077_002006"
     CDS             562..2820
                     /locus_tag="VW077_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9455952.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKVLKAVMTSKGNIQIKSA
                     KKTEVKSSEKEK"
BASE COUNT          927 a          605 c          602 g          790 t
ORIGIN      
        1 atctttaaac tagcagaatt agtgagaaat cttagttttt tctagtcaag ttggcctcct
       61 ttatggtaaa atagtaacga taagaaatga aggagaatca aaatggaatc acatttggtt
      121 agaatcatca accgccttga agcaatggca aacgatggcg gaaatttaaa acgtaatttt
      181 gaacgcgaag gagttgttgt tgcagaagtt gcgtacagcc acgacgaaga aaacggccca
      241 atctttaccc ttcgtgacgt agaagatcgc gaaacgtata cgtttgatag cattgactta
      301 atcgcaatgg aaatttacga acttctctac taatctaaaa caagctggga ttgtccctgc
      361 atgtctaagt aaaatccttt ttgtctcaca aataggattt ttttatagtc caaattcaca
      421 aaaattttca ttgtaacaac ttctcaaagc ttcaatcttt ttacttgctt tacaccccaa
      481 tcctgttata atgagagtat agaatttgac tatttttgac ctttaaacaa gtcaggctaa
      541 agaaagaaat gaggtagatg tatgctttgt caaaactgta aaattaacga ctcaacaatt
      601 catctttaca ccaatctcaa tggaaaacaa aaacaaattg acctctgtca aaactgctat
      661 aagattatca aaacagatcc taacaatagc ctcttcaaag gtatgacgga tctgaacaat
      721 cgtgacttcg atccctttgg tgatttcttc aatgatctaa acaatttcag accttctagc
      781 aatactcctc ctattccccc aacccaatca ggtggaggtt acggtggaaa cggcggttat
      841 ggttcccaaa atcgtggatc tgctcaaact ccgccaccta gccaagaaaa aggcctgctg
      901 gaagaatttg gtattaatgt aactgaaatt gcccgtcgtg gagacattga ccccgttatt
      961 gggcgcgacg atgagattat ccgtgtcatc gagattctca atcgtagaac caagaataat
     1021 cctgtcctta tcggtgaacc tggtgtcgga aaaacggccg ttgtcgaagg tctagctcag
     1081 aaaattgtcg atggcgatgt gccacataaa ctccaaggta aacaagtcat ccgtctggat
     1141 gtggttagct tagttcaagg aacggggatt cgaggacaat ttgaagaacg catgcaaaaa
     1201 ctcatggaag aaattcgcaa acgtgaagac atcatcctct ttatcgatga aatccatgaa
     1261 attgttggtg ctggttctgc gagtgatggt aatatggacg caggaaatat cctcaagcca
     1321 gcccttgctc gtggagaact gcaactagtc ggtgctacta ccctcaatga ataccgtatc
     1381 attgaaaagg atgctgccct cgagcgtcgt atgcagcctg ttaaagtcga tgaaccaacg
     1441 gtggacgaaa caatcactat tctcaaaggg attcaaaaga aatacgaaga ttaccaccac
     1501 gttcaatata cagatgctgc gattgaagca gctgcaactc tttccaatcg ctacatccaa
     1561 gatcgcttct tgcctgacaa ggccattgac ctcctagatg aagctggttc taagatgaac
     1621 ttgaccttga attttgtgga tcctaaagta attgatcagc gcttgattga ggctgaaaat
     1681 ctcaagtctc aagctacacg agaagaagat tttgagaagg cggcctactt ccgcgaccag
     1741 attgccaagt ataaggaaat gcaaaagaaa aagatcacag accaggatac tcctatcatc
     1801 agcgagaaaa ctattgagca cattatcgag cagaaaacca atatccctgt tggtgatttg
     1861 aaagagaaag aacaatctca actcatccat ctagccgaag atctcaagtc tcatgttatt
     1921 ggtcaagatg atgcagtcga taagattgcc aaggctattc gccgtaatcg tgtcggactt
     1981 ggtaccccta accgcccaat cggaagcttc ctcttcgttg ggccaactgg tgtcggtaag
     2041 acagaacttt ccaaacaact ggctatcgaa ctttttggtt ctgctgatag tatgattcgc
     2101 tttgatatga gtgaatacat ggaaaaacat agtgtggcta agttggtcgg cgctcctcca
     2161 ggttatgttg gctatgatga ggctggtcaa ttaactgaaa aagttcgcca caatccatat
     2221 tctctcatcc ttctcgatga agtggaaaaa gctcacccag atgttatgca catgtttctt
     2281 caagtcttgg acgatggtcg tttgacagac gggcaaggac gcaccgttag cttcaaggat
     2341 gccatcatta tcatgacctc aaatgcaggt acaggaaaga ccgaagctag cgttggattt
     2401 ggtgctgcta gagaaggacg taccaattct gtcctcggtg aactcggtaa cttctttagc
     2461 ccagagttta tgaaccgttt tgatggcatt atcgaattta aggctctcag caaggataac
     2521 ctccttcaga ttgtcgagct catgctagca gatgttaaca agcgcctctc tagcaacaac
     2581 attcgtttgg atgtaactga caaggtcaag gaaaagttgg ttgacctagg ttatgatcca
     2641 aaaatgggag cacgcccact tcgtcgaact attcaagact atattgagga cacaatcact
     2701 gactactacc ttgaaaatcc aagcgaaaaa gttctcaaag cagttatgac tagcaaggga
     2761 aacattcaga ttaaatctgc caaaaaaact gaagttaaaa gttctgaaaa agaaaaataa
     2821 atcctataga aaaggagtag aaaatgaaat ttttctgctt ctttttttac taaaataact
     2881 gtaatttctt gacagcttgc cctttgtcca ttatgatata tagt
//
LOCUS       DAVKGU010000135         2908 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKGU010000135 DAVKGU010000000
VERSION     DAVKGU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.137"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..1354)
                     /gene="rlmD"
                     /locus_tag="VW077_002007"
     CDS             complement(<1..1354)
                     /gene="rlmD"
                     /locus_tag="VW077_002007"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9455953.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1392..2168
                     /gene="recX"
                     /locus_tag="VW077_002008"
     CDS             1392..2168
                     /gene="recX"
                     /locus_tag="VW077_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9455954.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2257..2790
                     /locus_tag="VW077_002009"
     CDS             2257..2790
                     /locus_tag="VW077_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9455955.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          934 a          532 c          529 g          913 t
ORIGIN      
        1 aaactttttt tattaatttt acaacagctt cagttcgagc tgtatgtggg aacatatcga
       61 ccgactggat ataatgaaga tcatagactt ctactaagcg taccaaatca cgagccaagg
      121 tcgaaacatt acaagaaata taaaccattt tttctggtac ataagtaaga atagtatcta
      181 ataacttatc atccagacct gtacgtggtg ggtcaacaat caaagcatct gctcggtagc
      241 cttccttgta ccaacgagga ataatctctt ctgccgttcc agcttcataa tgagtattgt
      301 caaatcccat tcttttagca tttcgcttgg catcttcaat agcttctgga ataatatcca
      361 tacctctgag tgtttttact ttctttgcaa aggcaaatcc aatcgttcca actccacaat
      421 aagcgtcaat caaatggtct tctttatcaa catccagcgc ttttactgct tcgctataga
      481 ggacttctgt ttgctcagga tttagttgat aaaaagctcg aggggatagt gaaaattcat
      541 aattgagtac accttcttga atactctctt gcccccagat aatctctgtc ttttcaccat
      601 atatctcact ggttttagct gtatttgtat taacagctac tgtcacaact tctgggaaat
      661 ctttaaccaa ctcttttacc aattgagtta aattaagctg gcggtttgta acaataataa
      721 tctgaacctg tccggtcttt ctcgcgcgtc ggaccataat agtacggaca cctagaactt
      781 ttctctcatc cgtgattgga atctggtgat aagtaagtaa ttctgctaag cgattagcaa
      841 tcacttgggt ttccttatct tgtaccaggc agtctttcaa ctctactaaa tagtgagagt
      901 tttgtgcata taagcccgcc ttgacctgat ttttaaattt tcgggtctga aattgtaact
      961 tagctctgta atattttggt tcctgcattc caatagttgg acgaatttca taattttcat
     1021 atcctgcagg agcaaatttt ttcagcgctt gatgaagtaa gtccgtcttg aactccagct
     1081 gcttatcata atgcaggtgc atgatttggc agcctccgca ttcattataa atagtacaag
     1141 atggcacaat tcgaaattta gacttcttgt tgaccttcag taattttgct tcaacaaagt
     1201 tgcgtctaat agaagtaatc tgacaataga tatcttcgcc tttgagagct cctggtacaa
     1261 agactaatgt tttttggtaa aagccgattc cctcaccgtt aattcccatg cgcttgattt
     1321 ttaatggtat tttttgtttc actttcagat tcatacccct atcttatcac attttgagtt
     1381 ataatagagc tatgaaaatc acaaaacttg aaaagaaaaa aagactctat ctgatggagc
     1441 ttgataatgg cgacaaatgc tatatcaccg aagatacaat tgttcgtttt atgttatcga
     1501 gagataaggt gataagcgaa gaggaattga aagagattca agactttgct caattttctt
     1561 atggtaagaa tctggccctc taccacttat cctttaaagc acgcactgaa aaagaagtca
     1621 gagaatatct gaaaaaatac gatattgata aaaacatcgt ttctcaagtt attgctaatc
     1681 ttaaagaaga taagtggatt aatgatggtc agtacgctta tgctatcatc aatgccaatc
     1741 aactttcagg agacaaggga ccttatgtac tgactcagaa attagcacaa aaaggaattt
     1801 caaaatctac tatagaagaa atcttgaatg attttgattt ttcggaagtt gctcaacgtg
     1861 tagccaataa actattgaaa aaatatgagg gaaaacttcc agctcgtgcc ctgcaagata
     1921 agattatcca gaacttgact aacaagggct tctcttattc tgatgctaaa attgcctttg
     1981 acgacttaga tagccaagtt gaccaagaaa cgactcaaga actcatcttc aaggaacttg
     2041 ataagcaata tactaagtat gctcgaaagt atgaaggata cgaactgaag cagcgtttaa
     2101 ctcaagtttt agcacgaaag ggctacgatt tttcggatat agcaagcgct cttagagaat
     2161 atctttaata tttccaagtg aaattcacag attttaggta attttatggt acaatagtaa
     2221 atgataaact tataaattgt agaaagttgg ttagttatga agcttccaaa agaaggcgac
     2281 tttattacaa ttcaaagtta taagcatgat gggagtctcc accgaacttg gcgggacacc
     2341 atggtactaa aaacaacaga aaacgccatt attggtgtca acgatcatac acttgttacc
     2401 gaaagtgatg gtcgtcgttg ggtcactcga gaaccggcta ttgtttactt tcacaagaaa
     2461 tattggttta atatcattgc catgattcgt gataatggaa cttcttacta ttgcaatatg
     2521 gctagcccct actatctgga tgaagaagca ctgaagtata ttgattacga tttggatgtt
     2581 aaaattttta cagatgggga aaaacgtctc ttggacgttg aagagtacga gcgtcacaaa
     2641 cgcaaaatga attattctga tgacttggac tatattttaa aagaacatgt caaaattctt
     2701 gttgattgga ttaacaatgg acgtggtcct ttctcagaag cctatgtaaa catttggtac
     2761 aagcgctatg tagaactaaa gaatcggtaa agttgtcaaa ggtcaggaac aaaatcctga
     2821 cctttttttg gtagtataaa ataaattgtg taaacacaaa aaggaataaa tctattatag
     2881 cagagttaca acacattact agaaagag
//
LOCUS       DAVKGU010000136         2779 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKGU010000136 DAVKGU010000000
VERSION     DAVKGU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.138"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(431..1198)
                     /locus_tag="VW077_002010"
     CDS             complement(431..1198)
                     /locus_tag="VW077_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEU9455956.1"
                     /translation="MDNKRLIHAVAGSGKTTKIIETIDPQKRNLILTYTETNQNTIRA
                     KLIEKFGYIPESTFIFGVFESLYSFCLVPYLGKRPKGINFDYKTQGKFDKTAIDNTGR
                     IVQNQLSKSLLRGSLIYKRKEIRFDNSYLDRIDKFFDCIYVDECQDFESDDFDWLLSL
                     SNLNAEVFLFGDYYQKTFSTSRRGNKGKRIHSSFENWLKVISDSGYEIDLSSLSKSYR
                     CPKIVCDFIVDNLAIEILSQREEKLSSQITLIDSQKK"
     gene            complement(1180..2769)
                     /locus_tag="VW077_002011"
     CDS             complement(1180..2769)
                     /locus_tag="VW077_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9455957.1"
                     /translation="MISRIILQNFKRYQNVDFTCNSEVNIFVGENGAGKSTLLYGIGL
                     VLSGSHSQIERSSLSSIINQEAILEFMGTRDINQLPEVFIEIYFDELSTEISSNFNIE
                     GKHNSQKIKAFGLSLKIIPNQDYIAEITSVLNDSKWTVFPFEFYRVEFLTFSGKTYSS
                     YNKPYKFLHSMINTSLIDTQQEIQKRIHEVYLDNISAENRAKVNHQYRINSFNFLKTL
                     RKDELLKENASEFQLHFDESENSFRNSVSAVKNGVDIKNLGQGEKVLLGVENAYSHLK
                     ETVKILLIEEPENHLSFQNLQKLVRMLSSNTGVQVFIGTHSNMIASRLGVDNLLFLDN
                     GQISKLKGLKKDTVRFFKKSTNQNLLNFSLGKKIILVEGNAEYILVEKFFEILHSKKP
                     EEFNVSIISVDGLSFERYLEIATNFTDKKVVVITDNDGDYESKVQSKYSSYQQFQNIK
                     VSSDLDNDNRTFEICIYNCNSDLLESSGLTKSNDLLEYMLREKAEFALRLLEQMETDN
                     IREKFIIPNYIKEAIEWIIKD"
BASE COUNT          934 a          491 c          334 g         1020 t
ORIGIN      
        1 cccaatgcaa caagacttgt ggaatattac tgataaagca aataagaatt ggaatctcca
       61 aaaaaattga tataatatac tgccatttca ttggtctttt tataaataaa attttaacta
      121 ttctcatatt ctctcctaat ttgatatttc ccttacatca tcttcctata tttttctata
      181 tcggattgct ttacaaaata taagttcccc ctcgtacgtg tacacgcaac gtaaaattta
      241 cttttggttt gtgaagaaag ttcattcaag agatcagctg cgaataattt gtatgatgtt
      301 ggatttaata ctacacaaac attgtcatat tcagagcctt tactatcccc ccaattctga
      361 ctcttgcatt cataatcata agatttttga taaaaaagtt tcataatatt gtcatctgtc
      421 attattgatt ctattttttc tgtgaatcga taagagtaat ttgggaagat agcttttctt
      481 ctcgctgtga taaaatttca atagctaaat tatcaacaat aaagtcacaa actattttgg
      541 gacaacgata actttttgac aaactcgata aatcaatttc atatcctgaa tcagaaataa
      601 cttttaacca attctcaaaa ctcgaatgga tcctttttcc tttattacct cgccttgaag
      661 tactaaaggt tttttgataa taatctccaa ataaaaatac ttctgcatta agattactta
      721 aacttaaaag ccaatcaaaa tcatctgact caaaatcctg acactcatct acataaatac
      781 aatcaaaaaa tttatcaatt ctatccaaat atgaattatc aaatctaatt tctttcctct
      841 tatagattaa tgaacccctt aatagagatt tagaaagttg attctgaaca attcttccag
      901 tattatctat agcagtctta tcaaattttc cttgagtttt ataatcaaaa tttattcctt
      961 ttggtcgttt ccctaaatat ggcactaagc aaaaactata aagagactca aaaacaccaa
     1021 aaataaaagt agactcagga atgtagccaa atttttcaat caactttgct ctgattgtat
     1081 tctggttcgt ctctgtatac gtaaggataa gatttctttt ttgaggatcg atggtttcaa
     1141 ttattttagt tgttttgcca cttccagcaa cagcatgaat caatctttta ttatccattc
     1201 aatcgcctct ttaatataat tgggtataat aaatttttcc cttatattat cagtttccat
     1261 ttgttctagt aatctcagtg caaattcagc cttttccctg agcatgtatt ctaataaatc
     1321 attagatttg gttaatcctg aagactctaa taaatccgag ttacaattat aaatgcatat
     1381 ctcaaatgtt cgattatcat tatctaaatc agaagaaact tttatattct ggaattgttg
     1441 ataagaacta tattttgact gtactttact ttcgtaatca ccatcattat ccgtgataac
     1501 tactactttt ttatcagtaa agttagttgc aatttctaaa tacctctcaa aacttaatcc
     1561 atctacagaa ataattgata cattaaattc ttcaggcttt ttggagtgga gtatttcgaa
     1621 aaacttctct actaaaatat actcagcatt cccctcaaca agaattatct tcttacctag
     1681 agagaagttc aataaatttt gattagtaga tttcttgaaa aatcttacag tatctttttt
     1741 caaaccttta agtttagata tctgaccatt atctaaaaaa agaagattgt ctacacccaa
     1801 tcttgaagca atcatatttg aatgagtccc aataaaaact tgaacacctg tatttgagct
     1861 tagcattcta accaatttct ggaggttttg gaagctcaaa tgattctcag gttcctcaat
     1921 aagtagtatt ttaaccgttt ccttgagatg actgtatgca ttttcaacac ctaataatac
     1981 cttttctcct tgccctaaat tttttatatc tacaccattt tttacagctg atactgaatt
     2041 tctaaatgaa ttttctgatt cgtcaaaatg aagttggaat tctgaagcat tctccttcaa
     2101 cagttcatcc ttccttaaag tctttaaaaa attaaatgaa tttatcctgt attgatgatt
     2161 aacttttgct ctattctcag ctgatatatt atccaaatat acctcatgaa tacgtttttg
     2221 aatttcttgt tgtgtatcaa taagacttgt atttatcatt gaatgtagaa acttatatgg
     2281 tttgttgtaa gaggaatagg ttttccctga aaatgtcaaa aattcaactc tataaaactc
     2341 aaatggaaag actgtccatt tactatcatt taagacagaa gtaatttcag ctatataatc
     2401 ttgattggga attattttta atgaaagtcc aaaagctttg attttttgag agttatgttt
     2461 cccttcaata ttgaagttag aggaaatttc agtacttaat tcatcaaaat aaatctcaat
     2521 gaagacctca gggagctgat ttatatccct tgtacccata aattctaaaa tcgcttcttg
     2581 attaatgata ctagacaaac tacttctttc aatttgactg tgactaccac tcagcactaa
     2641 accaattccg tataatagag ttgatttccc agcaccgttc tccccaacaa aaatattaac
     2701 ttcagaatta caagtaaaat caacattctg ataacgttta aagttttgta atattattct
     2761 tgatatcata cagactacc
//
LOCUS       DAVKGU010000137         2746 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKGU010000137 DAVKGU010000000
VERSION     DAVKGU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.139"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(292..849)
                     /gene="sdbB"
                     /locus_tag="VW077_002012"
     CDS             complement(292..849)
                     /gene="sdbB"
                     /locus_tag="VW077_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9455958.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGQEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            complement(860..1567)
                     /gene="ccdA2"
                     /locus_tag="VW077_002013"
     CDS             complement(860..1567)
                     /gene="ccdA2"
                     /locus_tag="VW077_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9455959.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            complement(1798..2214)
                     /locus_tag="VW077_002014"
     CDS             complement(1798..2214)
                     /locus_tag="VW077_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455960.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGMEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            complement(2239..2439)
                     /locus_tag="VW077_002015"
     CDS             complement(2239..2439)
                     /locus_tag="VW077_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455961.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            complement(2612..2746)
                     /locus_tag="VW077_002016"
                     /pseudo
     CDS             complement(2612..2746)
                     /locus_tag="VW077_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          814 a          578 c          494 g          860 t
ORIGIN      
        1 aaaatctgac taccgtaact tctcaaagta acttccatct ctctcccaaa actggaagtt
       61 actctaagac gactctgttt tagggctatt tgtcgtgcac tttttctgag tcttttcgct
      121 tttgaaccct tatatatcaa gaaaaagaaa accagtgaaa gttagtctaa gacggatttc
      181 cactgatgtc acgcgttttt ctcatactaa aagctaagtt gtgggaaact ggaagttagt
      241 tttagaagtt accaaaatct gactacatct tgtaatcaga ttttatcgat tctagttcat
      301 ttctttaaat gctgcttctg catccgcatt actgatagca ccaaattgaa tctttccaat
      361 ctttccttgg ctatcaatta aatattctgt aggaatgctt cgaatttgat aagcttggaa
      421 ggtggttgct ttggtatcat aaagaactgg gatatcctta tatccttgtt cctggaacca
      481 ttgtgggaat tgctcaacag ttttttcacc ttgaattcct ggtgcaatga cagtaagaat
      541 ttcgaaatca cgatctggtt tcgccgctag ttccatcaac tctggcatac ttttcttgca
      601 tggaccacac catgaagccc aaaacttcaa gtaaaccttt ttacccttaa aatcagataa
      661 cttaacttct ttgccatcca tggattgcaa tgtaaagtct ggagcatctt ttccaacagc
      721 aatttgttgt acagtcgttt gttgttttgg ctgttgtgct gcttgagtct ttttagtttc
      781 ttcctgacca caggccatca atacaactaa tgacaagaga cttaagccag caaacattac
      841 ttttttcatt tgtcctcctt tattcaaaaa ttccagctag aacatttact tgtcctaata
      901 gtaacaaaat tcccattaaa acaatgagga aaccaccaat tttctttagt agcatcatat
      961 gacgcttgat tttactaaaa tatggcatga ctagacctga agctagtgcc aagaccaaga
     1021 aaggaagggc catgcccaga gtgtaaatga gagtataaat cgctccttgc caagcaccat
     1081 tgcctccaga agccgcaagt gctaaaacag aacttaaaac tggaccaata caaggtgtcc
     1141 aaccaaagct aaaggtaata ccaagtaaaa aagctgacca ataacgatta gaatctgatt
     1201 ttttaaaggt aaaacttttt tgaacttcta atttcttcaa atgaaaaatt tccatctggt
     1261 gaagacccaa aatgataata atagctccca tgacatatcg aaaccaattt gcatagagaa
     1321 tatgaccaaa gtaaccagca ccaaagccta gaataaagaa aatgagagag ataccagcga
     1381 taaagcaaag tgttcgaatc aagcctgacc agagaacctt tctcccaaac aaagaaaagc
     1441 tttttgcact ttcttgatca tccaataaaa tcccagcata gactggcaga agaggaaaaa
     1501 tacaaggaga aaaaaaggat aaaacacctg ctagaaaaac agagattaaa aatactatcg
     1561 tttccaataa agaaccaact ttcttaataa ttctaatcct attttactat attcaatttt
     1621 atttgtaagc tttctgctac gcaaaatcgt atcgggcact attggaccaa tcttttcttt
     1681 tgctagtcaa ggcggatctt atcccccaaa atagccaaaa agcaacgaca aggattactc
     1741 gtcactgctt ttgtgaacga aaatgtcttt taggtctgac atttcataaa tcatgtttta
     1801 cttgagtttg tcaaggattg ctttaagctc ctctactagt ttagtttctg tctctgctga
     1861 gccattttct tctttcacga aatcaagggt ttcttggaga aggttttggg ctttggcaag
     1921 gactttttta tccgcttttt ctgcatctag ctgtcctaga accttgatca attccgtgct
     1981 taattgctgg atttctgact ctttcttacg gcgaatcagc cagaaggcaa tcacgcctag
     2041 gagggcaagt agactgacca caatcactcc tgccggaact gagtttgttt cagtcatctt
     2101 atctgaatcc ttactatctt ccattccttg ttttgcatcc ttcttgtcct gtgcaggctt
     2161 gctgtcgcta gcatttgctt tcacatcttt gagagagtcc aaggcagcct tcacagactc
     2221 tactgcagta tgcagacctt actctgtcaa ggcactatct tccggagctt tttgagcatc
     2281 taggaggaca gccttggttg catcgatttt tggatcagat actgttgcca aagctttcaa
     2341 gcgttggtct aactcttgac tcaaggcacg aagttcagac ttgtcaactt gctcttgagc
     2401 ttgtgtgctc gttgagctag ccgaagcgct tgctaccact ctaggatctt gagtcggagc
     2461 tgagcttgga gctgggacag gacttgcagg ttgactagga gcagttatgg tatattgaaa
     2521 ctagaatagt acatatagac ttctaaaaca ttgttagaat tcgattttac tgtcctgatc
     2581 gatttgtcct gttcttattt cattttactc taataaccga tggtgtggtt aatgttggta
     2641 agagaaactt ctgaaaccaa gcttcaaaaa agtcgctcgt catcgtctct tcgtaagtca
     2701 ttggagcgat taactcacca tttgttagac ctgcaaccaa agaaat
//
LOCUS       DAVKGU010000138         2683 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKGU010000138 DAVKGU010000000
VERSION     DAVKGU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.140"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..2586
                     /locus_tag="VW077_002017"
     CDS             <1..2586
                     /locus_tag="VW077_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9455962.1"
                     /translation="KFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDGS
                     QSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSEE
                     LLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEHV
                     KDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLSA
                     SELAAAKAHLAGKNMQPSQLSYSSTPSPSLPINPGTSHEKHEEDGYGFDANRIIAEDE
                     SGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSNAKEM
                     KDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVGIGHS
                     HSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVSFSFP
                     PELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFKYTIA
                     SKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDALVRVF
                     DEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYIVEVP
                     ILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTSNSTL
                     EEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTGEAPQ
                     GNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPEGNVG
                     SDPMLDSALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVIKKNL
                     SDLIA"
BASE COUNT         1021 a          417 c          531 g          714 t
ORIGIN      
        1 aaatttagta aaaaatatat agcagctgga tcagctgtta tcgtatcctt gagtctatgt
       61 gcctatgcac taaaccagca tcgttcgcag gaaaataagg acaataatcg tgtctcttat
      121 gtggatggca gccagtcaag tcagaaaagt gaaaacttga caccagacca ggttagccag
      181 aaagaaggaa ttcaggctga gcaaattgta atcaaaatta cagatcaggg ctatgtaacg
      241 tcacacggtg atcactatca ttactataat gggaaagttc cttatgatgc cctctttagt
      301 gaagaactct tgatgaagga tccaaactat caacttaaag acgctgatat tgtcaatgaa
      361 gtcaagggtg gttatatcat caaggtcgat ggaaaatatt atgtctacct gaaagatgca
      421 gctcatgctg ataatgttcg aactaaagat gaaatcaatc gtcaaaaaca agaacatgtc
      481 aaagataatg agaaggttaa ctctaatgtt gctgtagcaa ggtctcaggg acgatatacg
      541 acaaatgatg gttatgtctt taatccagct gatattatcg aagatacggg taatgcttat
      601 atcgttcctc atggaggtca ctatcactac attcccaaaa gcgatttatc tgctagtgaa
      661 ttagcagcag ctaaagcaca tctggctgga aaaaatatgc aaccgagtca gttaagctat
      721 tcttcaacac cttctccatc tcttccaatc aatccaggaa cttcacatga gaaacatgaa
      781 gaagatggat acggatttga tgctaatcgt attatcgctg aagatgaatc aggttttgtc
      841 atgagtcacg gagaccacaa tcattatttc ttcaagaagg acttgacaga agagcaaatt
      901 aaggctgcgc aaaaacattt agaggaagtt aaaactagtc ataatggatt agattctttg
      961 tcatctcatg aacaggatta tccaagtaat gccaaagaaa tgaaagattt agataaaaaa
     1021 atcgaagaaa aaattgctgg cattatgaaa caatatggtg tcaaacgtga aagtattgtc
     1081 gtgaataaag aaaaaaatgc gattatttat ccgcatggag atcaccatca tgcagatccg
     1141 attgatgaac ataaaccggt tggaattggt cattctcaca gtaactatga actgtttaaa
     1201 cccgaagaag gagttgctaa aaaagaaggg aataaagttt atactggaga agaattaacg
     1261 aatgttgtta atttgttaaa aaatagtacg tttaataatc aaaactttac tctagccaat
     1321 ggtcaaaaac gcgtttcttt tagttttccg cctgaattgg agaaaaaatt aggtatcaat
     1381 atgctagtaa aattaataac accagatgga aaagtattgg agaaagtatc tggtaaagta
     1441 tttggagaag gagtagggaa tattgcaaac tttgaattag atcaacctta tttaccagga
     1501 caaacattta agtatactat cgcttcaaaa gattatccag aagtaagtta tgatggtaca
     1561 tttacagttc caacctcttt agcttacaaa atggccagtc aaacgatttt ctatcctttc
     1621 catgcagggg atacttattt aagagtgaac cctcaatttg cagtgcctaa aggaactgat
     1681 gctttagtca gagtgtttga tgaatttcat ggaaatgctt atttagaaaa taactataaa
     1741 gttggtgaaa tcaaattacc gattccgaaa ttaaaccaag gaacaaccag aacggccgga
     1801 aataaaattc ctgtaacctt catggcaaat gcttatttgg acaatcaatc gacttatatt
     1861 gtggaagtac ctatcttgga aaaagaaaat caaactgata aaccaagtat tctaccacaa
     1921 tttaaaagga ataaagcaca agaaaactca aaacttgatg aaaaggtaga agaaccaaag
     1981 actagtgaga aggtagaaaa agaaaaactt tctgaaactg ggaatagtac tagtaattca
     2041 acgttagaag aagttcctac agtggatcct gtacaagaaa aagtagcaaa atttgctgaa
     2101 agttatggga tgaagctaga aaatgtcttg tttaatatgg acggaacaat tgaattatat
     2161 ttaccatcgg gagaagtcat taaaaagaat atggcagatt ttacaggaga agcacctcaa
     2221 ggaaatggtg aaaataaacc atctgaaaat ggaaaagtat ctactggaac agttgagaac
     2281 caaccaacag aaaataaacc agcagattct ttaccagagg caccaaacga aaaacctgta
     2341 aaaccagaaa actcaacgga taatggaatg ttgaatccag aagggaatgt ggggagtgac
     2401 cctatgttag attcagcatt agaggaagct ccagcagtag atcctgtaca agaaaaatta
     2461 gaaaaattta cagctagtta cggattaggc ttagatagtg ttatattcaa tatggatgga
     2521 acgattgaat taagattgcc aagtggagaa gtgataaaaa agaatttatc tgatctcata
     2581 gcgtaaggaa tagcagtaga aaaagtctga atcaaaaatg aagttctctc aaaagttaga
     2641 aataaaactc tgactttggg agaatttcat tttattatta ata
//
LOCUS       DAVKGU010000139         2661 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKGU010000139 DAVKGU010000000
VERSION     DAVKGU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.141"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            161..1507
                     /gene="gdhA"
                     /locus_tag="VW077_002018"
     CDS             161..1507
                     /gene="gdhA"
                     /locus_tag="VW077_002018"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9455963.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEMMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHGGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVDQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2003..2173)
                     /locus_tag="VW077_002019"
     CDS             complement(2003..2173)
                     /locus_tag="VW077_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455964.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2186..2632)
                     /locus_tag="VW077_002020"
                     /pseudo
     CDS             complement(2186..2632)
                     /locus_tag="VW077_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          837 a          506 c          461 g          857 t
ORIGIN      
        1 atatttccaa gaaaaacgtt cgttttttta tctattttca aaaaaaccca aaaactgaat
       61 tgaaagaatt tttagaaaat ttctgttttt tctttacagt gaaaaaaaat tctgctataa
      121 tgtcttatgt aataaaatat gacaataaaa ggagaacgat atgacatctg ctaaagaata
      181 tatccaaagc gtgtttgaaa ctgtgaaagc tcgtaacggg cacgaggctg aattcctcca
      241 agctgttgaa gaatttttca acactttaga acctgtattt gaaaaacacc ctgagtatat
      301 cgaagaaaat atcttggcac gtattactga gcctgagcgc gtggtttctt tccgtgttcc
      361 ttgggttgac cgtgatggaa aaattcaagt aaaccgtggt taccgtgttc aattcaactc
      421 agctgttgga ccatacaaag gtggacttcg tttccaccca actgtaaacc aaggaatctt
      481 gaaattcctc ggatttgaac aaatctttaa aaatgtcttg actggacttc ctatcggtgg
      541 aggtaaaggt ggatcagact tcgatcctaa aggtaaaaca gatgctgaaa tgatgcgctt
      601 ctgccaaagc ttcatgactg aattgcaaaa acacatcgga ccatcacttg acgtacctgc
      661 tggtgatatc ggtgttggtg gacgtgaaat tggttacctt tacggtcaat acaaacgtct
      721 taaccaattt gatgctggtg tcttgactgg taaacctctt ggatttggtg gtagcttgat
      781 tcgtccagaa gcaactggtt acggtttggt ttactatact gaagaaatgc tcaaagctaa
      841 cggtaacagc tttgctggta agaaagtcgt tatttcaggt tcaggtaacg ttgctcaata
      901 tgctcttcaa aaagcaactg aactcggtgc aactgttatt tctgtttctg actcaaatgg
      961 ttatgtcatc gatgaaaatg gtatcgactt cgatcttttg gttgatgtta aagaaaaacg
     1021 tcgtgctcgt ttgactgagt atgcagctga aaaagcaact gctacttatc atggaggttc
     1081 tgtatggact tacgctggta actatgatat cgctcttcca tgtgcgactc aaaacgaaat
     1141 caacggtgaa gcagctaaac gtttggttga tcaaggcgtt atctgtgtat ctgaaggtgc
     1201 caacatgcca agcgaccttg atgccatcaa agtttacaaa gaaaatggta tcttctacgg
     1261 acctgccaaa gctgccaacg ctggtggtgt agccgtttca gcccttgaaa tgagccaaaa
     1321 cagtcttcgc ctctcatgga ctcgtgaaga agttgatggc cgtctcaaag acatcatgac
     1381 aaacatcttc aacacagcta aaacgacttc agaaacatac ggtcttgata aagactacct
     1441 tgcaggagct aacattgctg cctttgaaaa tgtagcaaac gctatgattg cacaaggtat
     1501 tgtttaagat ttatttactc aatcctcaat cactacgatt ggggattttt agttgagtta
     1561 gacagttggg aaattctaat taaatgaaat aaaaactgaa caaatttatt gcggaattca
     1621 aaccaatttc taacatattt ctagaaatta atttgacttt tctaatcgat ttgttcatat
     1681 cttatttcaa tttactatac ttacttaacc ctttttatac tcaatgaaaa tcaaaaagca
     1741 aactaggaag cgagtcgcag gctgtacttg agtacggcaa ggcaaagctg acgtggtttg
     1801 aagagatttt caaagagtat taaacaaaaa gaaaaaccgc tactcctaag aatagcgatt
     1861 taatcatgtt tttaagctac tactgtagtt gctctattat ttaagtgtta tgaataactt
     1921 caatatcttt aaagcttttt gttagattta taatttcaac tatagccatc tctactcctt
     1981 agtttacagc aaaaaattat gcttaattct tattgttttt gtaaacaatc agcgaaccca
     2041 atactattcc attcaacaag cctaaagaaa taccatattc aactggaaaa tctaaaaaaa
     2101 ttgcaaacac tattccaatc atcaccccta gtgaggctgt tgttaaaatt acatattcat
     2161 tttctttctt cattttcttt acacctcatt tcctttttgt atataagctg tagtactctc
     2221 caccaagatc cattaagtac ttatgatttc ctgactcaac aatcttacca cctttcatta
     2281 caaaaataat atccgcatcc ttaatcgttg acaatctatg agttacaatt atgtgagtac
     2341 agccctgact ttttatatac tttgttattc tttcctcatt aatagtgtct aaagcactag
     2401 ttgcttcatc taaaattaca atactaggat tatttattaa tgcacgtgcc agtgctatcc
     2461 gttgcctttg cccacctgaa atatttgacc ccatctctga gatgatagta ttaaatttca
     2521 tcggcatagc catgatttca tcatagattt gaactgcttt acaaacttcc tatatctttt
     2581 gtgaagtaac ttcgtgcttt aaagttatat tatcaagaat acttcggttc aataaaaatg
     2641 aatcctgtgg aactactcct a
//
LOCUS       DAVKGU010000140         2661 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKGU010000140 DAVKGU010000000
VERSION     DAVKGU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.142"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(166..780)
                     /locus_tag="VW077_002021"
     CDS             complement(166..780)
                     /locus_tag="VW077_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9455965.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1594..1863)
                     /gene="rpsO"
                     /locus_tag="VW077_002022"
     CDS             complement(1594..1863)
                     /gene="rpsO"
                     /locus_tag="VW077_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9455966.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2004..2633)
                     /locus_tag="VW077_002023"
     CDS             complement(2004..2633)
                     /locus_tag="VW077_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9455967.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNGKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
BASE COUNT          829 a          531 c          449 g          852 t
ORIGIN      
        1 ttcttatttc attttactat atttataaga atctcctaat caaatcaaaa gccactctac
       61 aatagagtaa ctttaagatt gtccatttat actcaatgaa aaagccctct gaaaatcaga
      121 gagctgattt gagctcggac taaaatccta gtgaaacaaa aaaatctaca cgatcagaaa
      181 agtctctatc gtgccatttt catacatgat gtatagtcca agtagaatga atactaaggg
      241 cacaatgatt cgctcgtatt tttcaattgt ctcgaatatt aacggaatag aggataacac
      301 ccgactaatc tcgcaaaaga taattatgcc gattacaaac acaagcaagg ccacgagggt
      361 ctgtgaccaa tctaacgaag caaaataagg tatatagata cctaaattat ctccgccaga
      421 cgcaattgtc agcaatgtaa ctgtccaaaa cagttgattt gccttgcttt gttctaatct
      481 ttcaataatt tcttcctctt cttcttccgc atcttctcca acaattgcaa agcgaatccc
      541 taaatagata gggattaaac caagcaatcc aaccatccat tcttcaggca cgaaattaac
      601 gacataagca gcaactaaac tcgcccctac aagtaagcct gtgcctagat attgccccgc
      661 ataaatatgc catttctgtt tattctgtga tagctgtgca aataaaataa ttaaaataat
      721 taaataatcg atactggtgg aaatataaac accaatagca gatatgattg tctgtcccat
      781 aaaaaaaact cctgtattag tcagtaataa atcagcagat tttaggagag cacagacaca
      841 ttaatttagc tatcgctagt gggtaatgtc gaacgtagga aagcatttta ctcctcctca
      901 aaacgtagtt acaaagtaat ttctaactgg aattcagtga ctacttccat gtaaagtata
      961 acatactata ctttactttg taaagagtgg gctcttctag ggggcttgca tacagctact
     1021 cggcttgaaa agccgagtaa ctgcgcacct ttatggtgta attagctgat accgtttgag
     1081 atttttgtag tctccaaaat tgttaccgat aaaacctcaa taaaaaagcc ctctgaagtc
     1141 agagagctga tttgagcccg ggctaaaatc ctagtgaaaa agatgaaact ccttgtgctc
     1201 atcgaacact gtgtcatttc cctattttca tacggatttt tgacgccctt agcatcatga
     1261 ttcttgctag ataaatcgtt tatagactag gcgacgaggc gaagatcgta cgaaactttc
     1321 agtgaaacaa aaaaatctcc ccgaagggag aaaagctggt ttattttacc ttacagtgct
     1381 aggaaccgta accaaagatt atacttgagt atatcgaggt aaggtgacaa cgcaaaaaag
     1441 ctacccaatt tagagtagct ttattatcaa gatatgctca attgaacacg gcctaagcac
     1501 ttggcaaaaa agataaaatc tcctagaaac tgaagtttct tcgtcaattt tcctattttt
     1561 gctttgtgct tttgacagca tttgtatctt gaattagcga cgaagtccta gagagttgat
     1621 caactcacgg taacggttaa cgtcgttttt acgcaagtat gcaagcaagt tacgacggcg
     1681 accgattttt ttcatcaatc cacggtaagt agcgtggtct tttttatgtt gtttgatgtg
     1741 ttcgttaagg tgattgattt cccaagtaag gacagcaact tgaacctcta ctgaacctgt
     1801 atcaccttcg tgacgtgcat attgtgcaat gatttcattt tttttctctt ttgagattgc
     1861 catgatgttt ctccttttta tttggcttca tccgagtgac aggttggcat gcctgtaacc
     1921 aagaagaagt tatttgtctt tacgacaatc cttattttac caagatttaa attttctgtc
     1981 aaacattaat actctcatag ttattatttt tcaagtaaag agatacgttc ttttatatga
     2041 tgaccttttt caagttcatc taccaatcca atagcataat ctgcataact aatctggctt
     2101 attccttttc cattagtagt aaaaatttct cctgctagaa tgtagtttcc tgttttttca
     2161 ccatcgggaa taaaatctac agcaggactg acaaaagtcc agttgagatt attttttgta
     2221 cgaagaagat ccaattcatc cgcttgagct tttgctagtg acttaaattc ttcaggaaaa
     2281 tcagacgtat ctaaaagtcg tgtcgttttt gtttcatcta tataaagact tcctgctccc
     2341 cctacaacta gaaaacgtgt ctgtgttcca gcaagaagct gattaaatag ttcgattgat
     2401 ttgctgtgga gcggtagcgt atctggtgta taagcaccaa acgctgaaat aacagcatca
     2461 aatccagtaa gatcatcttt tgtcaactca aataaatctt ttttaataat agactcagct
     2521 tgacttttgt tttcagaacg aacaatagcc gttacttcat gtcctcgttt gactgcttct
     2581 tcaacaattg ctttccccgc ttgtccattt gctgcaataa ctgctagttt cattttttat
     2641 acctctcttg ttgtaattat t
//
LOCUS       DAVKGU010000141         2611 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKGU010000141 DAVKGU010000000
VERSION     DAVKGU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.143"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..1631
                     /locus_tag="VW077_002024"
     CDS             <1..1631
                     /locus_tag="VW077_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9455968.1"
                     /translation="KIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGEYE
                     DAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQDYG
                     VNLICVEDDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNGGFA
                     PYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKNP
                     VYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSDEVWQAAQVKLKSQA
                     KKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKYKDFYYYGCKHRQM
                     IRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMKVDTSEIEKEIDNY
                     QKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDKIDELESSLIDAKA
                     KKQTIEAEKLTGDNIYKILIYFDKLYKVMNDVERRQLISALISEIQVYEEKQSNGQWL
                     KSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            1899..2465
                     /locus_tag="VW077_002025"
     CDS             1899..2465
                     /locus_tag="VW077_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455969.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT          973 a          334 c          582 g          722 t
ORIGIN      
        1 aaaaaataaa agtatacctc tatacacgag tatctacgtc aatacagata gaggggtatt
       61 cgttagaggc acaaaaatca cgaatgaaag cttttgctat ttacaacgat tatgaaattg
      121 ttggagagta tgaagatgcc gggaagtctg gcaagtctat tgaaggtaga atacagttca
      181 atcgaatgat ggaagatata aaatctggaa aagatggagt atcttttgtt cttgtgttta
      241 agttatcaag atttgcaaga aatgccgctg atgttctttc tacacttcag ataatgcagg
      301 attatggagt taatcttatc tgcgttgaag atgatggcat tgattcatct aaagatgctg
      361 ggaaactaat gatttctgtt ttatcagctg tggctgaaat agaaagagaa aacatccgta
      421 ttcaaacaat ggaaggtcgc attcaaaaag caagggaagg aaaatggaat ggtggctttg
      481 ctccgtatgg atataaactt gaagacggca agctgtttat aaatgaggaa gaggcagttg
      541 caataagaac gattttcgac cagtatgtaa atactacgat aggagccaat gggatatcta
      601 aatacttaga gaatcatgga attagaaaaa tcccaagaca gaatggtaag aatcctgtat
      661 ataatgggaa aatagcattt ggaagaagaa ctttagaaaa agttcatggt acaagaaatg
      721 aatataagca ggttgaacaa gatgaatatc taatatctga agggatacat gaagctatag
      781 tttccgatga agtttggcaa gctgctcagg ttaagctaaa atctcaagca aagaaatatg
      841 agcatgtgaa taaaggaaaa gatacacgca cacacttgct ttctggaatt gtaaaatgcc
      901 cgatatgtgg agtgggaatg tttgggaaca agtgtatcaa gaaaaagaaa gatggcacaa
      961 agtataaaga tttttattac tatggttgta aacataggca gatgataaga ggtcataagt
     1021 gtacattcag taagcaaatt agagaagaat tgttagatga tgctgttgca gaggtgattg
     1081 tcaagatagt aagtaatccg aaatttgctt ctatgatgca agaaaaaatc aacatgaagg
     1141 tggatacctc tgaaatagaa aaagaaatag ataattacca aaaggaattg agaaagagtc
     1201 attctacaaa gtttaagcta attgaggaaa tagataattt agatgttgaa gataagcatt
     1261 ataagagaag gaaacaggat ttagatgata gactatatcg tatgtatgac aaaatagatg
     1321 aattagaatc atcactaatt gatgcgaaag caaagaaaca gactattgaa gctgaaaagc
     1381 ttacaggaga taacatatat aagattctga tctattttga taagctctat aaagtcatga
     1441 atgatgtaga gcgtaggcag ttaatttcag ctttgatttc tgaaattcaa gtttatgaag
     1501 aaaaacaatc taacggacaa tggctaaaat ccattacttt taagctacca attatagaag
     1561 aaaatctcaa cataggtttg gacaatgatg aacaagttga atgtgtaagt ttattagaga
     1621 aacgtagtta atcctcaaaa ggttccccaa accttttgag tcccactgac gcatcacgct
     1681 gaggcggtat cactgcttgt acgagttgaa gcatcagcga agtaaaagta gatgttcagc
     1741 caatgcgacg aggtgagacc gaaggtcgat aaggtgtgtt ctgggtatag gactcgtaaa
     1801 gtaagtagga aatatgatga gatgatacag agagcctgaa ttggtcaggt gacgtgctta
     1861 tttacattag cctgataatg aaaatggaga gattatcgat ggttatattg gaattgtacc
     1921 aaaatgatta ttctaaagat ttagtagcat ttgattccat agaagatggg aaagcctttg
     1981 tagctcaaat cccgggatac actctggaaa cagaagatgg ttttgaagtg gagtatttta
     2041 atcctaaaaa cataccggat tatatggaaa ttatttttaa cggaaatatt gttcctttat
     2101 ctaaatttat gttcgatcct gaggaaaatg taaacattat ttggaaagag atttcaaatt
     2161 tatccctcaa aaatgataga gtgatcgaag gatattcaaa aattgatgca tatgtcgtta
     2221 acaaccatga agttaaagcc tatgtcgaaa caagagaaac gaactatcgt aaagcaaaag
     2281 acttcttaga aagccgcgga tatgaaatcg acagaagctt tttcggaagt gaagacggtg
     2341 aagcgattct ttacagaaaa aaagggatcg aagtttggca tttcttgtgt cacttagacc
     2401 caatgtttgt agagattgaa gatgtagaag gatatgttaa agaagaagtt ggagagattc
     2461 aatagtcaac caaagttcct tgtgagtata tacctttgct agtaaaagag tcaaaacgtt
     2521 ataacgaagg gggggaagaa aacccttgag tgtaagtctt tttcttgttt agagggaaag
     2581 tattagagta gccgctcata atttatacta g
//
LOCUS       DAVKGU010000142         2520 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKGU010000142 DAVKGU010000000
VERSION     DAVKGU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.144"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            114..536
                     /locus_tag="VW077_002026"
                     /pseudo
     CDS             114..536
                     /locus_tag="VW077_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(748..1140)
                     /gene="rpsI"
                     /locus_tag="VW077_002027"
     CDS             complement(748..1140)
                     /gene="rpsI"
                     /locus_tag="VW077_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9455970.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(1159..1605)
                     /gene="rplM"
                     /locus_tag="VW077_002028"
     CDS             complement(1159..1605)
                     /gene="rplM"
                     /locus_tag="VW077_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9455971.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1962..2279
                     /locus_tag="VW077_002029"
     CDS             1962..2279
                     /locus_tag="VW077_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9455972.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
BASE COUNT          775 a          483 c          460 g          802 t
ORIGIN      
        1 aagaaaatta agataaaact aaaaggactt agtcctgtgc aatacagaac taaatccttc
       61 ggataaatta attgtctaac tttttggggg cagtacataa gaaccttggt aatatgcgtt
      121 ttattatggc aacatttccc attttctcta tttctaatga cttacttcaa ctccttacct
      181 cttggaaaga acaacaaaga tatgaactag caaagtttgg catcctcagt aatccagaac
      241 aatttgtttt tacatatatc gacacaaaag gaaacatcaa caagccttta catgccgact
      301 atctgaacaa taaaatgaaa agcattagaa agcgacataa agaactgaca cacgctacac
      361 ctcacaaatt acgccataca ggcgcaactc tagctaaata agcaggaatg agcttagaag
      421 ccatttctga agcactaacg catagcgata caggcacaac tcaaatttat gtcaacacct
      481 ctaatgtagt tcctatgaca gtaggtgaat ttgctttaaa gtctctaaaa caataaggta
      541 tagtagattg aaactagaat agtacacctc tacttctaaa acattgttag aattcgattt
      601 gactgtcctg atcgatttgt cctattctta tttcatttcg ctataaaaat aaggtaaact
      661 ttgagataaa cttttcaaaa aaacacgaaa aaagcaccca taaggtaaac ttttgagtgc
      721 tttgaaacgt tgatataatc gtattgatta acgtttacta aattgtgatg ctttacgagc
      781 tttcttaaga cctggtttct tacgttcaac tttacgtgag tcacgtgtaa gaagtcctgc
      841 gcgtttcaat gaatcgcgga agtctgggtc tacttgaaga agggcacgag cgataccgtg
      901 acggatagct cctgattgac cagcgtatcc accacctaca acgttaacga aaacgtcgta
      961 tgaacctaca gttgaagtaa ctgcgaatgg ttggttgatg acaagacgaa ggtcagcgtg
     1021 tgggatgtac tcttcaacat cttttttgtt aacagtgatt ttaccagttc ctggaacaag
     1081 gcgaacgcgt gcaacagcgt ttttacgacg tccagtacct gcatattgtg cttgtgacat
     1141 actttattgt tcctttcctt agataagtcc tgaaatgtca agaacttctg gttgttgtgc
     1201 agcgtgagtg tgctcagctc caacaaatac tttcaacttc ataccttgag cgcgtccaag
     1261 tgtattgtgt ggaagcatac ctttaactga tttctcaatc aaacgtactg catttttaga
     1321 acgaagttca cctgcagaga tttgtttcaa tccacctggg tggtttgagt gagtgtagta
     1381 gattttatca gttgcttttt taccagtcaa tttaactttt tcagcattga taacaatcac
     1441 gaagtcacct gtatcagtgt gtggtgtaaa tgttggtttg ttttttccgc gaagtacgct
     1501 agcaactact gcagaaagac gtccaagtgg tacatcagtt gcgtcaacta cgtaccattt
     1561 acgttcaact tggcctggtt tagccataaa tgttgttttg ttcatgattt ctcctatata
     1621 tagttcgatt tttgtttacg gtgatgggtg ttccttccca tcgaaaagta tttggaaggt
     1681 tccggggcct ttcaaatggg gtaaacaata ccgcctacta tcataccaaa tttcacccct
     1741 aaaagtcaat agatatgaaa aatatttttc ggtaacttct caaagtaact gaaagttata
     1801 taatcaagtt aatttgggaa tttacctatt aacttcggta gatttttgag aatttttttc
     1861 cttctttttc cttgtagtta aaatcctttt caaaaaatca ccttttcttg atttttaatc
     1921 ctatttgctg tatgataagg gaaaagaaag gggacagaga tatggctttt accaataccc
     1981 acatgcgatc tgctagtttt ggcattgtta ccagcttgcc tgatgacatc attgactctt
     2041 tttggtatat tatcgaccat ttcttaaaaa atgtctttga attggaagaa gaactcgagt
     2101 ttcaattgct taataaccaa ggaaagatta ccttccactt ttcaagtcaa cacctcccta
     2161 cagccattga ttttgacttt aaccatcctt tcgaccctcg ttatccccca agagtactgg
     2221 ttttagacat ggacggtaga gaaactatcc tcctcccaga agaaaatgac ctattttaat
     2281 acttaatgaa aatcaaagag caaactagga agctagccgc aagctgtact tgagtacggt
     2341 aaggcgacgc tgacgtggtt tgaagagatt ttcgaagagt ataaaaactc tagccttcag
     2401 ttgcaagtaa ctgaaaacta gagtttttct attttttcag gctctataat atttgtagtg
     2461 ggtaaatccc ctatagatat tatggagctt atttttgtgt agaaaaaaag tcccatatga
//
LOCUS       DAVKGU010000143         2434 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKGU010000143 DAVKGU010000000
VERSION     DAVKGU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.145"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            284..1288
                     /locus_tag="VW077_002030"
     CDS             284..1288
                     /locus_tag="VW077_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU9455973.1"
                     /translation="MNASFRGSASFIRDNVLVTAAHVVYDRDSRTEADSVYVIPAATP
                     NENPVGKIKVKEVRYLKEFQNTASNELTTYDLAVLILEEPIGAQLGTLGLPNNLGNLS
                     GTGATITGYPAVNSAKQMYTDSKDIVKDTGDFLMYQIDTLGGASGAGVYDSSFRLIGV
                     HVSGSSAGQMNSAVKLNEKALSFIYSVIQGHSIDGWKLISGTWYYYKNSTKQTGWQKI
                     NDTWYYLDASGKMRTDWEKVNGKWYYFESSGSMATGWKYVRGNWYYLDNKNGDMKTGW
                     YKDGSTWYYLDPTNGNMKKGWIKVGGNWYYLNSSGAMVTGSQTIDGKVYNFASSGEWI
                     "
     gene            1307..2287
                     /locus_tag="VW077_002031"
     CDS             1307..2287
                     /locus_tag="VW077_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9455974.1"
                     /translation="MKLLKKTMQVGLTAFFFGLLATNTVFADTTGGQFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGNKGEMVVGWQYLEIPGTGYRDNLLDNQPVNEIGLQHKW
                     YYFGQDGSLQEYVGWKELDAKDSLNVGKEYGEPFEGPEVLKYANYYFDKDHSLKTGWF
                     YDQSNWYYLAKTGYLGKDYLGGERRAGWIYDGSSWYYLDTTTAAMQTGWQYLGNNWYY
                     LRSSGAMATGWMKDASTWYYLDGQNGDMKTGWAYVGNSWYYLRSSGAMSTGWMKDGST
                     WYYLNASNGDMKTGWFQVNGKWYYAYGSGALAVNTTVGGYYVNYNGEWVK"
BASE COUNT          809 a          317 c          538 g          770 t
ORIGIN      
        1 gggtatttca attattgcga gtactttatt ggctagccag ttgtcgacag tatctgcttt
       61 gactgttatt tctagtacag gtgaagaata tgaggtaagt gaaacattac aagagagtcc
      121 aggaactaat aatttttcac ttcccgatat ttcaccgact tatggtaaat attatacaaa
      181 tcagtcagaa gtcattattg gcaacaatga tttagttaag attgggaata ctttacagta
      241 tccttattct acatcagctt acgtaaaagc agaatttaat agtatgaatg ctagttttag
      301 aggcagtgct agtttcataa gagataatgt tttggttaca gcagctcatg ttgtttatga
      361 tcgtgattct agaacagagg cggattctgt atatgttatc cctgcggcta ctccgaacga
      421 aaatcctgta ggaaaaataa aggtaaaaga agttcgttat ctaaaagaat ttcaaaacac
      481 agcttcaaat gagcttacta cgtatgattt agcagttttg attttggaag aaccaattgg
      541 cgctcaatta ggtacactag gtcttccgaa taatctaggg aatctttcag gtacaggggc
      601 tactattact ggatatcctg ctgtaaatag tgctaagcaa atgtatactg actctaagga
      661 tattgtaaaa gatactggag attttctaat gtatcaaata gatactctag gaggtgctag
      721 tggagctggc gtatatgaca gcagttttag attgattggt gttcatgtgt ctggtagttc
      781 tgctggacag atgaattctg ctgttaagtt aaatgaaaag gctttatcat ttatctactc
      841 tgttattcaa ggacattcta ttgatggttg gaaattgatt agtggtacat ggtactatta
      901 taagaacagt actaaacaaa caggttggca gaaaataaat gatacttggt actatctaga
      961 tgcttcaggt aagatgcgta cagattggga aaaagtaaat ggaaaatggt attactttga
     1021 atcatctggt tcgatggcaa caggttggaa atatgtacga ggcaactggt attacttaga
     1081 taataaaaat ggtgatatga agactggttg gtataaagat ggttcaacat ggtactatct
     1141 agatcctact aatggaaaca tgaaaaaagg ttggataaaa gtaggaggaa actggtatta
     1201 tctaaattca agtggagcaa tggttacagg tagccaaact atcgatggta aagtttataa
     1261 tttcgcttca tctggtgagt ggatctaata ttggaggggt atataaatga aacttttgaa
     1321 aaaaactatg caagttggac taacagcatt tttctttggt ttgttagcta caaatacagt
     1381 atttgcagat accacaggtg gccaatttgt tgataaggac aatagaaaat attatgtaaa
     1441 agatgatcat aaagcaatct attggcataa aatagacgga aaaacttact attttggtaa
     1501 taaaggagaa atggtagttg gatggcaata cttagaaatt cctggaacag gttatcgtga
     1561 taatttatta gataaccaac cagttaatga aataggcctt caacacaagt ggtactattt
     1621 tggacaagat ggaagtctcc aagaatatgt tggttggaag gaattagatg ctaaggattc
     1681 attaaatgtt ggcaaagagt atggtgaacc atttgaaggt cctgaagttc ttaaatatgc
     1741 aaattattac tttgataaag atcattcttt aaagacaggt tggttttatg atcaatccaa
     1801 ctggtactac ttagcaaaaa caggttattt aggaaaagac tatcttggtg gtgaaagacg
     1861 tgcaggttgg atatatgacg gttcaagctg gtattatcta gatacaacaa ctgctgcaat
     1921 gcaaacagga tggcaatatc taggtaataa ctggtattat ctccgttcat caggtgctat
     1981 ggcaactggt tggatgaaag atgcttcaac ttggtattat ctagatggtc aaaatggtga
     2041 tatgaagaca ggttgggctt atgttggcaa cagttggtac taccttcgtt catcaggtgc
     2101 aatgtctact ggttggatga aagatggttc aacttggtac tacctaaatg caagtaatgg
     2161 agatatgaag acaggctggt tccaagtcaa tggtaaatgg tactatgcct atggttcagg
     2221 tgctttagct gttaatacca cagtaggtgg ttattacgta aactacaacg gtgaatgggt
     2281 taagtaatga aggctaatgt taaactatag ttgatactta acatagtaaa agagagaatc
     2341 agagtgtgat tctctctttt gtaatttaaa atgattttaa gaaagttttt atatgtgttt
     2401 gaaaaattgt ataatctatg tgatggatta aatt
//
LOCUS       DAVKGU010000144         2424 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKGU010000144 DAVKGU010000000
VERSION     DAVKGU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.146"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            118..1125
                     /locus_tag="VW077_002032"
     CDS             118..1125
                     /locus_tag="VW077_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9455975.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMRVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALKRLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1128..1328
                     /locus_tag="VW077_002033"
     CDS             1128..1328
                     /locus_tag="VW077_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9455976.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1480..1662
                     /gene="rpmF"
                     /locus_tag="VW077_002034"
     CDS             1480..1662
                     /gene="rpmF"
                     /locus_tag="VW077_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9455977.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            1678..1827
                     /gene="rpmG"
                     /locus_tag="VW077_002035"
     CDS             1678..1827
                     /gene="rpmG"
                     /locus_tag="VW077_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9455978.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2319..>2424)
                     /locus_tag="VW077_002036"
     CDS             complement(2319..>2424)
                     /locus_tag="VW077_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9455979.1"
                     /translation="VSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT          801 a          463 c          441 g          719 t
ORIGIN      
        1 tcacctttta aaagaaatta ttgggctgaa actttttagt gattattttc aactttctat
       61 tgacttttaa aaacaaagtg atatcattta gatatcaaat aatgggaggt cattcttatg
      121 actgaaaaac taatcaattc aaaaccaaat ggtgtattcg cattgattct cattgagttg
      181 acaatcgtac ttggtatctt tatatttata atgagggttg gttcggaaaa catttttgga
      241 attattatcg gacctttact aatcgtaatt gcaggtctag ctcatgctgg tttaaaagtt
      301 gtcaaacctc aggaagctct ggttctgaca ctctttggta attatacagg taccatcaaa
      361 gaacctggtt tttactttgt caatcccttc agcgtagcag tcaaccctgc aaaccacact
      421 cgacttggac aaagtggtga tgttagcaca aaatctcctt ttttaggagc taaatcatca
      481 aatgacaatg atgtaaatct tgaaattggc aagaaacaca tttccctcaa agtcatgacc
      541 ttgagcaatt ctcgtcaaaa aatcaatgat tgcttaggaa accctgtaga aatcggtatc
      601 gcggtaactt ggagagttgt cgataccgct aaggcagtct tcaacgttga taactacaaa
      661 gaatatcttt cactgcaatg tgatagcgcc ctccgtaata ttgtccgcat ctatccttac
      721 gatgtgtctc ctaatgtgga tactacgggt gacgggcaag cagatgaagg tagtctccgt
      781 ggctctagcg aaattgttgc taaccgtatt cgtgaagaaa tccaaagtcg tgttgaggat
      841 gctggcttgg aaatccttga agcacgtatc acttacctag cttatgctcc agaaattgct
      901 gccgttatgc ttcaacgcca acaagcatct gccattattg atgcacggaa gatgattgta
      961 gatggtgctg taggaatggt tgaaatggca ctaaaacggc tcaatgaagg ggaattggta
     1021 gaacttgacg aagaacgaaa agctgccatg gtttcaaatc tccttgtcgt tctttgtggc
     1081 aatcatgatg cacaaccaat tgtcaacaca ggaagtcttt actaaagatg gctgaagcta
     1141 aaaaaaagca gatccccctt cgactctcaa caaagttata cgctgcactc gcatcatggg
     1201 cagaagacga ctttcgttca gtcaatgggc aaatcgaata tctcctcaca gagtgtgtca
     1261 agcaacggaa gaaagacgga aaatacgtat cagaaaccat tgacgaacca tttgagattg
     1321 atatttaaca ccttctcctg tctgccgtgt cggataggag aattttttca tctttttttg
     1381 tcaagtaact ttactttaca aaaaaaatgt gttatcctag tatggttgat gaaaatcagt
     1441 agattgaatc gaatataaat acctaaagga gaaatcaaaa tggcagtacc tgcacgtcgc
     1501 acttcaaaag cgaagaaaaa caaacgtcgt acacactaca aagtaacagc tccatctgta
     1561 aactttgacg aaactactgg agattactca cgttctcacc gtgtatcact taaaggatac
     1621 tacaaaggac gtaaaatcac taaagctgca tcagctgaat aatagaaggg agataccatg
     1681 cgcgtaaata ttacacttga acacaaagaa tctggtgaac gcttgtacct tacttctaaa
     1741 aacaaacgta acactccaga ccgccttcaa ttgaagaaat actcaccaaa acttcgcaaa
     1801 cacgttgtgt ttacagaagt taagtaagca ttcaatacga atcaatacag aagaaaaacg
     1861 cttgatcagc gttttttctt tttgttaatt tcactatatt gcaagggaaa tcattcgaat
     1921 gactacattt ttcactacct tttttgaaat aagaatacgg attcggacga ttaaaagtaa
     1981 gagaaactca aatgtaagtt ggggttcctt ttttgtatat ttttcaaata acgcctctac
     2041 actatttgta gcaaattcac caactacagt tgtatcttag ttaaaataag ttagaatatg
     2101 taagtgagta ccagatatac caagacatcg tcaccatcta aggtatattc aaaatacaaa
     2161 agttgaccaa ctagatttct gaatatcctt atatatccat tcttaaaatt ggtttaaata
     2221 gcgtagtctt ttaaactagt tttgagaatc caaaaaatct tcctacatat gtaagaagat
     2281 tttttagttc agaatgatta gatttagcta atggatacct atcctaccca ctcaccgtta
     2341 gcattgactc gatagccatc gggtgtagtc gtgtttacta ataaatctcc tgaattatag
     2401 gtatagtacc atttaccaga aact
//
LOCUS       DAVKGU010000145         2419 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKGU010000145 DAVKGU010000000
VERSION     DAVKGU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.147"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..861
                     /locus_tag="VW077_002037"
     CDS             <1..861
                     /locus_tag="VW077_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9455980.1"
                     /translation="SLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDID
                     LKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILI
                     DGKDIKEISTESLFNKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTD
                     FIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKI
                     QESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIE
                     KTKMAEEFIY"
     gene            872..1456
                     /locus_tag="VW077_002038"
     CDS             872..1456
                     /locus_tag="VW077_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9455981.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1456..2151
                     /locus_tag="VW077_002039"
     CDS             1456..2151
                     /locus_tag="VW077_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9455982.1"
                     /translation="MPTFSSNAPFNPNPISKILVIFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            2139..>2419
                     /locus_tag="VW077_002040"
     CDS             2139..>2419
                     /locus_tag="VW077_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9455983.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGKIIIDGKNIKNLELYQISMLVSTVFQNPKTYF"
BASE COUNT          867 a          318 c          462 g          772 t
ORIGIN      
        1 tctttagatg catctaaaga gggcttgatg gaaatatttt atttatcgcc caaaatagaa
       61 agattaaaag aaattcaaaa tcaagattta caagaaggcg atgactatag tttaaaaaaa
      121 tttgatattg atctaaaaga tgttgagttt gcctacaata aagacgcaaa agttttaaat
      181 ggtgtaagtt ttaaagctaa gcagggagag gtcactgctt tggtaggtgc aagtggctgc
      241 ggtaaaacaa ctatcttgaa acttatatca agactttatg attatgacaa gggacaaatc
      301 ttaatcgatg gcaaagatat aaaagaaata tcaacagaat ctctttttaa taaggtctct
      361 atagttttcc aagatgtggt tctctttaat caaagcatta tggaaaatat tagaatcgga
      421 aagcaagatg caagtgacga agaggttaaa agagcagcaa aacttgcaaa ttgcacagat
      481 tttatagaaa aaatggataa aggtttcgat acagttattg gtgagaacgg agctgagcta
      541 tcaggaggag aaagacaaag attatcaata gccagagcct tcttaaaaga tgcgccgata
      601 ttgatcttag atgagataac agcaagcctt gatgttaaca acgagaaaaa gattcaagag
      661 tctttaaata atttagttaa agataaaact gttgtaatca tttcacatag aatgaaatcc
      721 atagaaaatg cagacaagat agtagttctt caaaacggaa gagtagaaag cgaaggtaag
      781 catgaagagc ttttacaaaa atcaaaaatt tacaaaaatt taatagaaaa gacaaaaatg
      841 gcagaagaat ttatttatta ggaggactac aatggataat aaaaaattaa aagtaaaaga
      901 tttagtaagc atcggtgttt ttggcgtaat ttattttgcc ttcatgtttg gagttggtat
      961 gatgggcttg attccaatat tgttcttaat atacccgaca gtattagcca tagttgcagg
     1021 aactgttgtt atgttattta tggctaaggt tcaaaagcca tgggcactat ttatatttgg
     1081 tatgatatca ccacttgtga tgtttgcagc tggtcatacc tacgtagttg tggttttatc
     1141 acttatagta atgataatag cagaattaat tagaaagatt ggtaattata attcatttaa
     1201 atacaatatg ctttcttatg caatcttcag cacatggata tgtagctctt taatgcaaat
     1261 gcttttagca aaagaaaaat atatggagtg gtctttgatg acgatgggaa aagattatgt
     1321 tgatgtatta gaaaagttaa taacttatcc tcatatggct ttagtagcct taggtgcttt
     1381 cttaggagga attcttggag catatatagg caaggctcta ttgaaaaaac actttgaaaa
     1441 agcaggcatt gtataatgcc tactttttct tcaaatgctc cctttaatcc caatccgatc
     1501 tctaagatat tagtcatatt tatcacaggc ttaactgtta tgcatagcat aaacatccgt
     1561 tttgagctgg caattgtttg tcttattggt attttgcttt atttgaatgg atacaaaaaa
     1621 acacttttca aatggattat attgtgtggg atactttact ccttgcctaa ttttatggtg
     1681 ctatctgaat taaaccctat agttaagatg tttttgagta tacctattgt tattagaatg
     1741 tttattttac catttatggc agcaagcttt atgataaaga cctcggatgt aggcgcaata
     1801 atttcatcga tggataagct taagatttca aagaatgtat ccatacctat tgcggttatg
     1861 tttagattct tcccatcttt taaggaggag aagaaaaaca tcaaaatggc tatgagagta
     1921 agagggataa attttaaaaa cccagtcaaa tatcttgaat atgtttctgt gccactactc
     1981 attatatcat ctaatatatc agatgacatt gcaaaagcgg cagaaacaaa ggcaatagaa
     2041 aatccaattg ccaagaccag atacattcgc gtaaagatac agctaattga ttttgtttat
     2101 gttttagcgg ttgctggact tattgtggga ggcttaatat ggttgaaata aaaaatttaa
     2161 gtcttgatta tggtgaagag catatattag atgatatatc actatccata gccgagggag
     2221 agtgcgtgct atttacagga aaaagtggaa atggtaagtc atctttaata aattcaatca
     2281 atggactagc tgtaaggtat gataacgcaa agacaaaggg caaaataatt attgatggta
     2341 agaatataaa aaatttggaa ctttatcaaa tctcaatgct tgtttcaact gtttttcaaa
     2401 atcctaagac atatttttt
//
LOCUS       DAVKGU010000146         2408 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVKGU010000146 DAVKGU010000000
VERSION     DAVKGU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.148"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(75..788)
                     /locus_tag="VW077_002041"
     CDS             complement(75..788)
                     /locus_tag="VW077_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9455984.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1247..1621)
                     /locus_tag="VW077_002042"
     CDS             complement(1247..1621)
                     /locus_tag="VW077_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9455985.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGGIG
                     TSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
     gene            complement(2040..>2408)
                     /locus_tag="VW077_002043"
     CDS             complement(2040..>2408)
                     /locus_tag="VW077_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9455986.1"
                     /translation="DHVQRNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKP
                     ESPKPTEEPEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQ
                     DNNTIMAEAEKLLALLKENK"
BASE COUNT          724 a          482 c          390 g          812 t
ORIGIN      
        1 aatcaacttc agacatttca atgtactctt caatagtaat attagctttt tttgtagtca
       61 ttagctaatc tcctttaaaa tgtgatcttt tcaagaatat ttttatctaa acaatccagc
      121 aagtcttggt aagaatagac ttcgtaagtc ggctgggctt gtgtgtgatt ttcgaggtga
      181 tgaggattat accagatagt gtcaatcccc gcattattgc caccttgaat gtcggcggtt
      241 agagaatctc caatcatcag cgtcttttct ttactaaatc cagcaatttg ctggccaatc
      301 ttttcataaa agagagcatc cggcttttga gtttgcaact gttctgagat aaagacttga
      361 ttgaaataag gtactagacc agattgagcc aaacgtcctg tctgaatggc agtaatgcca
      421 tttgtcgcag catacaagtc ataatcacgc tcaatgaggc tgtccaagag atcatgagcg
      481 cccgatagtg tttgtccctg ctgagcgaga taaaattggt aacgctgggc aagaaaacta
      541 ccgtcttttt cctgtccaaa atgagaaaat aaacgagaaa agcgcgtgtt aaccagctct
      601 tgtttactga ttttcttcag ctccaagtct ttccagagag ccttgttcat aggaacgtaa
      661 taatctttat aagcctgaat atccgcaact ccttcttctt ttagaagttg ggtcaaagcc
      721 acatcctcag cagcatcaaa atcaagaaga gtgtggtcga ggtcgaagag tagaaatttg
      781 tagaacaatt tgaggttttc ctttctgaaa attcattaag aacattatat cataaagcac
      841 ctcatacaat taactaattt aatcacttaa aaaaaattcg aacactttct atacaactga
      901 cagctcaaat ctttcagaat agaacaatac taactatcga acaccccgtc ttcataaata
      961 catatgtaat tctaggccta gaattcctat aaactaaatg ctttcatact cttccaagta
     1021 attgattgcc ttaaatttta atttttgaag gattctaaag ctagaatagc cccatcacaa
     1081 tcagttttga ttgattcaca atttagaaac actatagttt cactcctgtt aaaataaaaa
     1141 ggaactgcat aaagcaatcc ctttctgatt ttgaaatcat ttacttaaca ttttatagtt
     1201 gagataatca atagcttacc tataaaaaga gttatagtaa aattccttat ttattgattt
     1261 caagctccgc taactgtgtt tgaataactg acagttctgc accagcctga agaagagcag
     1321 ctgcattata ggcaccttct acaattggaa ccctgttgat gatgatactt ttatcactga
     1381 aatcagtcac catttttaag ttcattttag cagaacctag gtcaaaaaag gcaagtaaag
     1441 tatctgctgg attttcggaa acaaccctat ctacttgatc aaaactcgtt ccaattcctc
     1501 cgccctcggt tcctcctaca taagtaatcg gaacatcttt agctacttta ctaatcagtt
     1561 caacaacacc ttctgcaatg tgtttggaat gtgaaacgat aacaagacca atactttcca
     1621 tcaaaccacc ccagtttcta aaatagcagt aaatagtaat cctgatgaga atgatccagg
     1681 atcgatatgt ccaaaaaacc acatgctcct aagacaagag ccaacagact ggccatcaat
     1741 aatagtattg ttctttttta tcattactcc ttaactagtg tttaactgat taattagcca
     1801 gtaaatagtt tacctttatt tacactatct gttaagatat agtaaaatga aataagaaca
     1861 ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggtatactat tctaatttca
     1921 atctactata ttttgcacat tttcataaaa aaatgagaac tagaactcac attctgctct
     1981 catttttcgt tttcccgttc tcctatcctg tttttaggag ttagaaaatg ctgctacctt
     2041 tacttattct cctttaataa agccaatagt ttttcagctt ctgccataat agtattgttg
     2101 tcctgggtgc caaatagtaa attatttttt aatcctgtga gagtctcttt ggcattggac
     2161 ttgataattg gatcctggat ttttccaagt aaatcttcag cctctctcag tttttcttca
     2221 accttttcag tctcgacctg aggttcttct ggttcctctg ttggttttgg agactctggt
     2281 ttctcgcttt gcggtttctc ttctcgaggg gtttcttcct caggtttttc tgtctgaggt
     2341 ttctcctcgc ttggtttttc cgtttgattg gtatcagctt gaccattttt gtttctttga
     2401 acatggtc
//
LOCUS       DAVKGU010000147         2305 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVKGU010000147 DAVKGU010000000
VERSION     DAVKGU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.149"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            105..1628
                     /locus_tag="VW077_002044"
     CDS             105..1628
                     /locus_tag="VW077_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455987.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGITLLALTIIGGLILGAMMGE
                     QEFMKIYQEALSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNYNIEFENVSFAYNDKAVIEN
                     LSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSIKIGGIAISEYSDEALIKAI
                     SFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDKFPERENTIIGSKG
                     VYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAFKNLMKDKTVIMIA
                     HRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            1612..>2305
                     /locus_tag="VW077_002045"
     CDS             1612..>2305
                     /locus_tag="VW077_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9455988.1"
                     /translation="MKEFYKKRFALTDRGARNLSKATLASFFVYCINMPPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVKGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERV"
BASE COUNT          822 a          309 c          439 g          735 t
ORIGIN      
        1 tagataagtt aataattaat tcaaacttat ccggtgcaga gagtatagca ttaaaatctg
       61 ttattacact aacaagtgga gcgatatttt attttgtctc aggaatgttt tcacatatct
      121 tgggatttag gcttgaaaca aatttaagaa aaaggggaat cgatggtctg gaaaaagcaa
      181 gttttaggtt ctttgactta aatccatctg gtcaaataag aaagatcata gatgataatg
      241 cagcacaaac tcatcaggtg gtagctcaca tgattccaga tagctctcag gcaatagtca
      301 cacctatact tgtccttgta ctaggcttta tagtaagtat aagggttggt ataactttgc
      361 ttgctcttac tataattggc ggcttaattt taggggcgat gatgggcgag caagaattta
      421 tgaagatata ccaagaagct ctatctaaac taagtgctga gactgtcgag tacgttagag
      481 gaatgcaggt tgtaaaaata tttaaagcaa atgtagagtc ttttaaaagc ttttataagg
      541 cgataaaaga ttactcaaag tatgcttatg attattccct atcttgtaaa aggccttatg
      601 ttttgtatca atggttattt tttggactga ttgcaatttt aattattcct atagtttatt
      661 ttatgactag cttagctagc gcaaaggtga ttttacttga gcttatcatg attttatttt
      721 tatcaggagt tctctttgtt tcattcatga gaatgatgtg gtactccatg tatatttctc
      781 aaggaaatta tgcagtagat actttagagg cgctttacga agatatgcaa aaagacaaat
      841 tagtgcatgg taatgtcaat aattttaaaa actataatat agaatttgag aatgttagct
      901 ttgcttataa tgataaagct gtcattgaaa atttatcctt taatttagaa gaaggaaagt
      961 cctacgcact tgtcggttca tctggatcag gcaaatcaac agtagcaaaa cttatatcag
     1021 gtttttacaa tgttaataaa ggaagcataa agataggcgg gatagcaata agtgaatatt
     1081 ctgacgaagc cttaattaaa gccatttcct ttgtttttca agattcaaaa ttattcaaga
     1141 agagcattta tgataatgta gctttagcta ataaagatgc gacgaaagat gacgttatga
     1201 gagccttaaa attagcagga tgcgatttaa tattagacaa attcccagaa agagaaaata
     1261 caatcatagg gtcaaaaggt gtttatttat ccggtggaga aaaacaaaga attgcaattg
     1321 ctagagcaat tttaaaggat tccaaaatta ttattatgga tgaagcatca gcatctattg
     1381 acccagataa cgagtttgag ttgcaaaaag ctttcaaaaa tcttatgaag gataaaacag
     1441 ttatcatgat tgcacacagg ctatctacaa ttaaagacct tgatgaaatt attgttatgg
     1501 atagtggaaa aattatagaa agagggtctg acaaagaatt aatgtcaaaa gatacaaggt
     1561 ataagagcct gcaagagatg tttaacagtg cgaatgaatg gagggtttca aatgaaagag
     1621 ttttataaaa aaagatttgc tcttacagat agaggagcaa gaaatttaag taaagcaaca
     1681 ctggcttcat ttttcgttta ttgtataaac atgcctcctg ccatattact tatgattttt
     1741 gctcaggaag ttttggaaaa tatgggcaaa agcaatggct tttatatagt attctcagtt
     1801 ttgactttga tagcaatgta tattttgctt tctatcgaat acgataaatt atataacaca
     1861 acctatcaag aaagtgcaga tttaagaata aggacagcgg agaatttatc aaaattacct
     1921 ctatcttact tttctaaaca tgacatttcc gacatttcac aaacaatcat ggctgatatt
     1981 gaaggcatag agcatgcaat gagccactca ataccaaagg tgggcggcat ggtactgttt
     2041 ttcccattaa tatctgtaat gatgctagcg ggcaatgtca agatgggttt agctgtaatt
     2101 attccatcta ttttaagctt tatatttata cctttatcta aaaaatatca ggttaaggga
     2161 cagaatagat attatgatgt cttaagaaaa aactcagaaa gctttcaaga aaatatcgaa
     2221 atgcaaatgg agattaaagc atataattta tcgaaggata ttaaagatga cttatataaa
     2281 aaaatggaag atagtgagag agtac
//
LOCUS       DAVKGU010000148         2217 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVKGU010000148 DAVKGU010000000
VERSION     DAVKGU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.150"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..193
                     /locus_tag="VW077_002046"
     CDS             <1..193
                     /locus_tag="VW077_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU9455989.1"
                     /translation="VKMMKPQKEIYLSLVNKYQLDIKDCIFLDDLEENVEAARTLGIK
                     AFQIKERKEISNILKDLLN"
     gene            203..>2217
                     /locus_tag="VW077_002047"
     CDS             203..>2217
                     /locus_tag="VW077_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9455990.1"
                     /translation="MVTIKDIARVSGVSHGTVSNVLNKKGNVSAAKIKLVEETAKKLG
                     YNMNSQAQFLRKGASSKCFFLLFNNARKKYAEYFAEIDQLSLDVEYVYLHKFSEIKNK
                     ISAILSENPQFIVCLGFSPKRFVDIEDNIPIFEVDTYQSNKTLSSFDSDKIFEIVLEL
                     VRTEKLSNLYFISLTNEKEHSLFEYLTQKIKNSTHIQVQERLQLLSLYPTLKNFAKYD
                     CIFVDDEDLMHTLLDLFDWFTIIDRPKICLIGSRVWFEKRNLFYIHLDYRILALNTAE
                     NLESGNSSIVPVKDGKQILTNQLFDGECDDIYLLTVHSPMNKALEILSEKYKQISGKN
                     IKIIEKRYDELLEMIESGDVSSSFDMIRMDMAWLPTFGKKYFQEITSFRQTKSINQNI
                     SKSVSREYMYIDQKQYTFPLDVSSQILVYRKDLFEDSFLQRRYFEKTKRKLTVPKSYQ
                     EFDELSEFFTLTENPFSPTLYGHSLALSSSVRAACEFIPRFRERLAQSRMNLAVLPQV
                     LTEYEKSYQFTEKSFNSSWNDFVTNLNSGKTSMEIIFSNYTSPLFDGLNVSAQFEFAT
                     ATIPGNTPVIGGGSIGISKYSNRVEECLNFINWLYSEEISILLTSLGGFLPSKYVMQN
                     RMLQFQYPWLSSLEDTFSIGSRTKWYGFNTDFRFEQMLGQELIESVK"
BASE COUNT          800 a          271 c          376 g          770 t
ORIGIN      
        1 agttaaaatg atgaaaccac aaaaagaaat ttacttgtca ttagttaata aatatcaatt
       61 agatataaaa gattgtatat ttttagatga tttagaagaa aatgtagaag ctgcaagaac
      121 tctaggtatt aaagcatttc aaattaaaga gcgtaaagag atctcaaata tactaaaaga
      181 tctcttaaac tagaggttaa ttatggttac aataaaagat attgcaagag tgtctggagt
      241 ctcacatggg acagtttcaa acgttttaaa taaaaaaggt aatgtctcag cagctaaaat
      301 aaagttagta gaagagactg cgaaaaaatt gggttataat atgaattctc aagctcagtt
      361 tcttcgaaaa ggcgcttcta gtaaatgttt tttcttgtta tttaataatg cacgcaaaaa
      421 gtatgctgag tattttgcag aaattgatca gttatcttta gatgtagaat atgtttactt
      481 acataaattc tcagagatta aaaataagat atctgcaatt ttaagtgaaa atcctcaatt
      541 tatagtttgc ttaggttttt ctccaaaaag attcgttgat attgaagata atattccgat
      601 ttttgaagta gatacatatc aatctaataa aactttatct tcctttgata gtgataaaat
      661 atttgagatt gtacttgagt tggttcggac tgaaaagcta tctaatttat attttatttc
      721 acttactaat gaaaaagaac attctttatt tgaatatttg actcaaaaaa taaaaaactc
      781 aactcatata caagtgcaag aacgtttaca attgttgtct ctatatccta ctctaaaaaa
      841 ctttgcaaag tatgattgta tatttgttga tgatgaagat ttgatgcaca cgctattaga
      901 cttatttgat tggtttacta taattgatag accaaaaata tgtttgatag gttcacgtgt
      961 ttggttcgaa aaaagaaatc ttttttatat acatttagat tatagaatat tggcactcaa
     1021 tactgccgaa aatttagaaa gtggaaatag ttcaattgta ccagttaaag atggcaaaca
     1081 aattttaact aatcaattat ttgatggaga atgtgatgat atttatttac ttactgtcca
     1141 ttcaccaatg aataaagcct tagaaatttt atcagaaaag tataaacaaa tttctgggaa
     1201 aaatattaaa attatcgaga aaagatatga tgaacttttg gagatgattg agtctggcga
     1261 tgtttcatcc agttttgata tgattagaat ggatatggct tggcttccaa catttgggaa
     1321 aaagtatttt caagagatta caagctttag acaaacaaaa agtattaatc aaaatatttc
     1381 taagtcggtg tctcgagagt atatgtatat tgatcagaag caatatactt ttccattgga
     1441 tgttagtagt cagattttag tatatagaaa agacttattt gaagattcat ttttacaaag
     1501 acgatacttt gaaaaaacaa aaagaaaatt aactgtacca aaaagttatc aagagtttga
     1561 tgagttaagt gaatttttta cattaacaga aaatccattt tcacctacac tttatggcca
     1621 tagtttagct ttaagttctt cagtgagagc agcatgtgaa tttattcctc ggtttcgtga
     1681 gagattagca caatctagaa tgaatcttgc tgtacttcct caggttttaa ctgagtatga
     1741 gaaaagttat cagtttaccg aaaaatcatt taattcttcg tggaacgatt tcgttacgaa
     1801 cttaaacagt ggtaaaactt caatggaaat aattttttca aattatacta gtccattatt
     1861 tgatggtctc aatgtttctg ctcagtttga gtttgcaact gccactatac ctggtaatac
     1921 tcctgtgata ggtggtggct caattggaat ttctaaatat tcaaatagag tggaagaatg
     1981 tctaaacttt attaattggt tatattctga agagatttct attttactaa catctctggg
     2041 tggtttttta ccatcaaagt atgtcatgca aaataggatg ttacaatttc aatacccatg
     2101 gttatctagt ttagaagata catttagtat tgggagtaga acaaaatggt acggctttaa
     2161 tactgatttt agatttgagc agatgttagg gcaagagtta atagaaagtg tgaagca
//
LOCUS       DAVKGU010000149         2093 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVKGU010000149 DAVKGU010000000
VERSION     DAVKGU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.151"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            531..950
                     /locus_tag="VW077_002048"
     CDS             531..950
                     /locus_tag="VW077_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9455991.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKTNCLLNLTAVESDKVRS"
     gene            965..1654
                     /locus_tag="VW077_002049"
     CDS             965..1654
                     /locus_tag="VW077_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9455992.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFSGVMIWILVKTGHGYLFRIPRKKWLWYSILTVLVLVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            1696..1929
                     /locus_tag="VW077_002050"
     CDS             1696..1929
                     /locus_tag="VW077_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455993.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
BASE COUNT          570 a          321 c          409 g          793 t
ORIGIN      
        1 tgcaacgggt gctgctacat tatgttaaaa atcgctcttg attgataagc tggatttgtt
       61 aggaaaggag taaataatta tggataagaa aaagatagtt tcgactataa tatgtatagt
      121 atttcttgta gtgtcagtag ataatttttt tagagactta accccgttac tgttcatttt
      181 aaatattatt ggcctgtctt gcttttcagt gctaacatac attaacataa aagagatact
      241 cttaaatata agtaaatgat gatttggtta gtcctagaac caatcaactt atgtttatcg
      301 gcttggcaga ttttatgttt gtcatttgtt tgtatagagg tatttcagaa acagaattct
      361 atcaacaact aatcgcttat ataggtgtat tctctgcttg tttatcacgc ttctgctcat
      421 gtggggcttg aagtattata aaaagtagct ttctatcctt ttctgacagg atatttgagt
      481 agaaggctct attaaattgt tagtgaaata aatgaatgga ggtatttaat atgaaataca
      541 gattattttt tgttattttc ttgagtagtg tgttggatat tcttttaggg acatttttac
      601 aaatctctat cgtatctatt gggtggcttg ttctttacag tggtttgttt gaagtaggag
      661 ttttccttct tgctaataaa ggggtggcgg taaaaatcaa ggaagtagat attcgaaatc
      721 gctttaaatt tatttttgga aaaaccttat ggtttcaaat tcttttgctc atctttttga
      781 taatcaaact ttatcttggt ttggatgcga ggttgatttt attctatgga catattttca
      841 ttgtctttaa tgccttaatg tatctattat ctagtagtca ggttagcctt aaaaaaacaa
      901 actgtcttct taatcttaca gcagtagagt ctgacaaggt gagatcatag tagggaggat
      961 ttctatgaaa aagtatcaac ttctattcaa aataagtgca gtcttctctt acttattttt
     1021 cgtatttggt ctttctcagc tgacgcttat cgtccaaaat tattggcaat tttcttccca
     1081 gattggtaat ttcgtctgga ttcaaaatat cttgagttta ctatttagcg gagtcatgat
     1141 ttggattctg gttaagacag gccatggtta tctctttcgc attccaagaa aaaaatggct
     1201 ttggtattcg attttgacag tattagtgct agtgctccag atctctttta acgttcagac
     1261 agctaaacat gttcagtcaa ctgctgaagg ttgggctgta ttgatcggtt atagtgggac
     1321 caactttgct gagctaggta tctatataac tttgttcttt ctgactccac ttatggaaga
     1381 gctaatctat agaggattac tgcaacacgc cttttttaag cattcgagat ttggccttga
     1441 tttgcttctt ccgtcaattt tatttgctct tcctcatttt tcaagcctgc ctagtttgtt
     1501 agatatcttc atctttgcaa catctggcat catctttgct agtttgaccc gctataccaa
     1561 gagcatttat ccttcctatg cggtgcatgt gatcaataat attttcgcaa cattaccatt
     1621 tttgctgact tttttacata gggtcttggg gtaaaaaaac aaaaggcttg cttttcagcc
     1681 atagaggaga ttatcatgta taaacactta tttttcctag attccaaaac tttagatcgg
     1741 ttgacaccct atattctagt cttggcttct gacaccattg cttttaatgt ttttgtgcta
     1801 acctttgtat ctgcggtggt ttttaatttc ctaaattcca tgctagcttt aatggctata
     1861 ttcatagggg ctggctatgt ggtcggattt tggttactaa tactcaatga aaatcaaaga
     1921 gcaaactagg aaactagccg caggctgtac ttgagtacgg caaggcgacg ttgacgtggt
     1981 ttgaatttga ttttcgaaga gtataaaatg gtttgttttg gaaaggctag aactaaagaa
     2041 tgacgcgtag gaagtctgtt tggtgaggag gatagtttta tggagttttt tga
//
LOCUS       DAVKGU010000150         2073 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVKGU010000150 DAVKGU010000000
VERSION     DAVKGU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.152"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(83..436)
                     /locus_tag="VW077_002051"
     CDS             complement(83..436)
                     /locus_tag="VW077_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9455994.1"
                     /translation="MDLVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            complement(492..1394)
                     /locus_tag="VW077_002052"
     CDS             complement(492..1394)
                     /locus_tag="VW077_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9455995.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(1602..1976)
                     /locus_tag="VW077_002053"
     CDS             complement(1602..1976)
                     /locus_tag="VW077_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU9455996.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALPAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          720 a          405 c          270 g          678 t
ORIGIN      
        1 tcatttataa tcgccctgaa tgataaaaat atatacaccc aacacacgat gatgtgtatt
       61 tttcgaatct tatctacatt actcatctta ctgttgcaga tatgaatcca tcccaaggat
      121 aagggttccc gttaatatca caacctctat taagaacacc atatccaata cagtaagcta
      181 ttttccctgc tgctgtagcc aatacacctg ctgctgcacc aataagccag cctccaagac
      241 ctccaacctt aatatagttt attagttttt tggttaaaac tgcagctcca gctaatgcta
      301 taaaagttcc tgaactcata gctaaagctg cgcaaattcc ttgacaagca cgcttgctta
      361 aataagctcc cccatcaaga tacatcattt cttcttcatc aatcacaaca taattatttg
      421 gtaatactaa gtccataagt tttctcctcc ttagttgaaa ttgtttcatt tgttttacaa
      481 ataggacgaa cttaaactat ctttttatta ctcagtaata atttccgaga tttcatttta
      541 tgatactctc tattcataca gaaatcaaaa ttttgcctta accaacttgt ataatagaaa
      601 aaatctaatt gatttattct gatgagctgt attctaattt tatcaacctt ttcttgccag
      661 ttgttattgt tactaataga aatctcatac agcagtaact cgacaagacc tgtacacaga
      721 agggtgttat gagaatcttc aattttactc agaatatcct caaagagttc actatccgtt
      781 aaaaaggtgg attggttcat cgcatcatac agctgaagag acattaagtc agaataactg
      841 agatctgatt tttgtatttt atcctttaac gtatttgtca aatcactttc aaacgaatct
      901 tctttttccc atttattttg attgaaaaga aattccgtct tattaaaaac gagatgattg
      961 atatccatta tagtccagaa aaatattgta gtaagcatga tgattgcaaa aggaattttt
     1021 aaaataaata ctaggataat agcaatttca agaataaaac tccatataat cctagatctt
     1081 aaatcatcag gcgacaaact tttacgatgg aatgaaaaat agggcgagaa tctcacattc
     1141 acttttttcc ttcttataaa aataaaggga aaaatcttaa taagaccttc tttcttgtat
     1201 aaatctttca atgcgtatag tatgtctaaa actatttttg ataaaatcaa actacagaaa
     1261 gcaagactca tactaatcac aaaatcaagg ggagattcta gattcaatag taagttattc
     1321 actaggacat gcaactctag ccacaagaca aaaagaggga gcattagtaa gaaaacaatt
     1381 agtttacgta tcataatttt acagtgtcct tatcattttc ttaattttgg ataaatcatc
     1441 attgctatga ttaaaatcaa aaaaacgaca ttaatccaaa taatcaactt ttttatttct
     1501 tcgctaacaa aatcataggc taaaaaagtt atggatgata taaatagaca tacaaaaaaa
     1561 ataataacaa aaattttttc cattttaata cccatatcta gttaccacaa ggcgtttaaa
     1621 tttgggtctc tcctatttac atcattattg gcccagattg ttgtaatagc atcgccaatt
     1681 gacatccaag ctgcaaatcc attaatagca ggcagtgcaa atcctgccac tctatttgca
     1741 actcgtaccc caacatatct tgcagctgct ccagcaattg cgcttgtaac tcgccgcaaa
     1801 ccataattac gaatataatt tctaactaga ctgattgctc ctccacctaa aacaccatta
     1861 aaagctatat taatggctgt tgcaacaggc catctcggaa tagaaaatcc cccatcaaga
     1921 tacatcatct cttcttgctc aagaacaaca taattatttg gtaatacgag ttccatttat
     1981 tttctccttt tttattaagc atagttagga tagtttccta acaagtgata gacgactgtg
     2041 tttttatcac ttagctacct agataagata tcc
//
LOCUS       DAVKGU010000151         1967 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVKGU010000151 DAVKGU010000000
VERSION     DAVKGU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.153"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            1..759
                     /locus_tag="VW077_002054"
                     /pseudo
     CDS             1..759
                     /locus_tag="VW077_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(875..1678)
                     /locus_tag="VW077_002055"
     CDS             complement(875..1678)
                     /locus_tag="VW077_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9455997.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYENATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          684 a          370 c          341 g          572 t
ORIGIN      
        1 tatcacttga aacagctaga caaaacagat aaggaccaag agcttaaagc tgaaattcaa
       61 tccattttta tcgaacacaa gggaaattat ggttatcgtc ggatttattt agaactaaga
      121 aatcgtggtt atctggtaaa tcataaaaga gttcaaggct tgatgaaagt actcaattta
      181 caagctaaaa tgcgacagaa acgaaaataa aggagacgtt ggtaagaagg cagagaatct
      241 cattcaaggc caatttgaag gatctaaaac aatggaaaag tgctacacag atgtgacaga
      301 atttgccatt ccagcaagta ctcaaaagct ttacttatca ccagttttag atggctttaa
      361 cagcgaaatt atcgcctata atctttcaac ttcacccaac ttagaacaag tacaaacaat
      421 gttggaacag gcattcacag agaagcacta cgagaatacg attctccata gtgaccaagg
      481 ctggcaatac cgacacgatt cttatcatcg gttcctaaga gtaagggaat tcaagcatct
      541 atgtcacgca agggaaacag ccaagacaac ggtatgatgg agtccttctt tgggattctg
      601 aaatcggaaa tgttttatgg ctatgagaaa acatttaaat cacttaatca attggaacaa
      661 gctattgtag actatattga ttactacaac aataaacgaa ttaaggtaaa actaaaagga
      721 cttagccttg tgcaatacag aactaaatcc ttcggataaa ttatttgtct aactttttgg
      781 ggtcagtaca cctaaaactt tgatgatata cgttttcttg tgagaatatt tacttcattt
      841 ttgcctaaaa ttcaatgttt actcagtatt tggattatga aaaatcgagg tctaaatcta
      901 gatacatttt ttctgaagac aaatcatttt gaccaccgag caagagattt tcaaaaaaag
      961 ctgttaaaaa ctcagaacgt cgctgtaaaa tctttgcatt atctaatacc aaggcatcac
     1021 gaaaatattt ggaatgttgc tgaaatggtg tattatcaat atcaaaacca aactcacgaa
     1081 gatactgaat caaaaagacc gttactgtcc gagtgtttcc ttcgcgaaat ggatgaatct
     1141 gccagattcc tgaaataaaa tgctggattt gtttaaccac atccgcctga gttagtgtcg
     1201 catatgcaac ttgtttttcc tgattaaaat cataatctaa ggtcatttga atcatggagt
     1261 aatcagagta cacaacactt tcaccattca aaacaggttc attctttgtg atattggtct
     1321 gacgaaattg ccccaccgga atagagggtt caaatatatc ttgaaacaac tccttatgaa
     1381 tagcaagtaa ggtcgcagga ctaaagctaa agcctcttcg agacaatagt tctacaatac
     1441 gtagagaaac caagtctgcc tcttccgtac ttgcatcaat agtatggtga taagccgttg
     1501 cattctcata aacctgctca taagtcagtt ctccccggga ctgtttctca gccaaagatt
     1561 ccatatacgc tgatggcact agattgtcaa ctttctgcag accaaaacct atccgccata
     1621 aatcacgctt cgcttcataa gacaagtttg gattgtcaat gttgtaagtt gattgcatag
     1681 aaatatcctt tcaattgttt ttcttcatta ccagtaaatc acgaaattgg ttggaaactg
     1741 gcttaaacta taaaaagtcc tcacaatagg ttctatgata tatatatata cttcatgtaa
     1801 ataataaaag agttataatc agcactactg ataaattcaa taccgatttc gccatcttaa
     1861 aacttagtca aacgaatacc gttgatttcc agttcattaa gtcgttgacc tgtcaagact
     1921 tctcgttcct tcaactggaa aagttaaggg ggaactaata caaatcc
//
LOCUS       DAVKGU010000152         1966 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVKGU010000152 DAVKGU010000000
VERSION     DAVKGU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.154"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..975
                     /locus_tag="VW077_002056"
     CDS             <1..975
                     /locus_tag="VW077_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9455998.1"
                     /translation="SACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVATI
                     AWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNFE
                     AISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGMEK
                     EGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANYL
                     TDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLLGK
                     INAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            965..>1966
                     /locus_tag="VW077_002057"
     CDS             965..>1966
                     /locus_tag="VW077_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9455999.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     "
BASE COUNT          690 a          285 c          356 g          635 t
ORIGIN      
        1 agcgcttgtt cttctaattc tgttaaaaat gaagaaaata cttctaaaga gcatgcgcct
       61 gataaaatag ttttagatca tgctttcggt caaactatat tagataaaaa acctgaaaga
      121 gttgcaacta ttgcttgggg aaatcatgat gtagcattag ctttaggaat agttcctgtt
      181 ggattttcaa aagcaaatta cggtgtaagt gctgataaag gagttttacc atggacagaa
      241 gaaaaaatca aagaactaaa tggtaaagct aacctatttg acgatttgga tggacttaac
      301 tttgaagcaa tatcaaattc taaaccagat gttatcttag caggttattc tggtataact
      361 aaagaagatt atgacactct atcaaaaatt gctcctgtag cagcatacaa atctaaacct
      421 tggcaaactt tatggagaga tatgattaaa attgattcaa aagccttagg tatggaaaaa
      481 gaaggtgatg agttaatcaa aaatactgaa gctcgtatat ccaaagaatt agaaaaacat
      541 ccagaaatca aaggaaaaat caaaggaaaa aaagtattat ttactatgat taatgctgca
      601 gatacatcaa aattctggat ttatactagc aaagatccaa gagcaaatta tttaacagat
      661 ttaggtctag ttttccctga atcattaaaa gaatttgaga gtgaagatag ttttgcaaag
      721 gaaatttctg cagaagaagc aaataagata aatgatgctg atgtaatcat aacttatggt
      781 gatgataaaa ctcttgaagc tttacaaaaa gatcctcttt taggtaaaat aaatgcaatt
      841 aaaaatggtg ccgttgctgt aattccagat aatacaccgt tagcagcctc atgcactcca
      901 acaccacttt caataaacta tactattgaa gaatacctaa atcttttagg aaatgcatgc
      961 aaaaatgcga aataaaaaac aaataaacct aggcataatt tttgtaatct gcctaggtct
     1021 tcttattaca atatttttgt cattaaagct tggaacaaaa gaaattaata tcagagattt
     1081 tttagcagct tttggaatgg gcaatacaaa tgatgatttt attaaatcaa ttatatataa
     1141 aagaatacct agaactattt ttgcaatttt agcaggttct agtcttgcca taagcggtgt
     1201 attgatgcaa tcagttacta gaaacccaat agctgatcca ggtatactcg gtataaacac
     1261 aggagcaagt cttagtgtag taattggtct ttctttttta ggaatttcat caagcataag
     1321 ccatataagt tttgcaatca ttggtggctt agtaagtgca atttttgtat acgcgattgc
     1381 tgtaagcgga aaagcaggcc ttacccctat aaaacttgcc ttatcaggaa cttgtgttag
     1441 tatggcttta agcagttttg taagtttttt aattttaccg aataataacg tcttagacaa
     1501 atttagattt tggcaaatag gtagccttgg agcagctaca ttatcttcta tatctacact
     1561 actacctttt ataattttag gtcacttgat agctatattt atttcatcag atttaaacgc
     1621 tttagctatg ggtgatgaaa tggctgttgg tcttggagtt aatgttaata ggataagatc
     1681 acttgcaata attgcaagtg tgcttttatg ttcaagtatt actgcaattg gtggacctat
     1741 tggcttcgta ggtcttatag ttcctcactt ttgtggctta tttataagca aagatatacg
     1801 cacaatgacc atttcttcat cttttatagg tgcagagctc ttgcttatat gtgatataat
     1861 cggccgtatg ttaggtaaac caggtgaaat tgaagtaggg ataattactg caataatcgg
     1921 gggtccagta cttatttatg taactatgaa aaatagaggg gttaat
//
LOCUS       DAVKGU010000153         1932 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVKGU010000153 DAVKGU010000000
VERSION     DAVKGU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.155"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..542
                     /locus_tag="VW077_002058"
     CDS             <1..542
                     /locus_tag="VW077_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9456000.1"
                     /translation="GESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKTSYLNMVSNV
                     LYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDDGRL
                     LSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVTHAP
                     HPELKQYFTKIYQFPKDFI"
     gene            891..1319
                     /locus_tag="VW077_002059"
     CDS             891..1319
                     /locus_tag="VW077_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456001.1"
                     /translation="MSEIVEMSKKISDLNDKKEFHKKNCELRFQDSVLDNINSVPSTT
                     HLFSCLNIQTLVKIILYIFRYNSCMKIFNFRDKELIFFNKIVNGLIQNIEENLEDDIE
                     RILKYLYICLFNEIFIIKNKVNFFDDVEFNQTLSEFLDKL"
     gene            1331..>1932
                     /locus_tag="VW077_002060"
     CDS             1331..>1932
                     /locus_tag="VW077_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9456002.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEK"
BASE COUNT          691 a          209 c          368 g          664 t
ORIGIN      
        1 taggtgaaag tggaagcggg aaatctacat tattaaaatt attgaatggc tttttgagag
       61 attatagtgg agaattgcga ttctgcgggg atgatataaa aaaaacctcc tatttaaata
      121 tggtttcgaa tgttctatat gtagatcaaa aagcttattt gtttgaaggt acgattagag
      181 ataatatttt attggaagaa aattatactg atgaagaaat actacagtct ttagagcaag
      241 ttggtttgag tgtaaaagat tttcctaata acattttaga ttattatgtt ggtgatgatg
      301 ggagattact gtcaggaggg cagaaacaaa aaattacttt agctagaggg ctaattagaa
      361 ataagaaaat agtattaatt gacgagggaa cttctgctat cgataggaga acttcgttag
      421 cgattgaacg taagatatta gatagagagg atttgactgt cattattgtt acccatgctc
      481 cgcatccgga acttaaacaa tattttacta agatatatca atttccaaag gattttattt
      541 aagaatatag gggagggaaa catgagattc tattgtgaag cttatgaggt aattactgaa
      601 gagaaaaaag tatactttaa tgatattgag ttagaagtta agtattcctc tgtcgaagaa
      661 ttgtttataa tatgtgagaa attattagat aagaaaaaag tatcattttt ttatgtagat
      721 gagaaaccct taaggtatct gttatttgac tatatatttc tactagttct agctaaaaaa
      781 atatcccaat cttatgaact gcaccccaaa agttagattt attctgtcta acttttgggg
      841 tgcagttcat tttgcatagt tttggcattc atcctagata tccatataaa atgtctgaga
      901 ttgttgaaat gtctaaaaaa atatcagatc taaacgataa aaaagaattt cacaaaaaga
      961 actgtgagtt gaggtttcaa gatagtgttc ttgataacat aaatagtgtc ccgagtacaa
     1021 ctcatttatt tagttgttta aatattcaaa cattagtaaa aattatttta tatatttttc
     1081 gatacaacag ttgtatgaaa atatttaatt ttagagataa agagcttatc ttttttaata
     1141 agattgtaaa tgggctgata caaaatattg aggagaattt agaggatgat attgaaagga
     1201 tattgaaata tttatatata tgtttattta acgaaatatt cattattaag aataaggtga
     1261 atttttttga tgatgtggaa tttaatcaga cacttagtga gtttttagat aaattgtaga
     1321 gggaatggtt atgagtaaca atactaagta tatcttttta tctccacatt tagatgacgc
     1381 aattttttcc tgtggtgatt atatttcaaa attaacttca gaaggagaaa ttgtacttgt
     1441 tattactata ttttctggtt accctttgag ccaacagcta cagccctctg ctaagcaatt
     1501 tcataagttg tgtaaccttg ggaaatatcc gattgaagaa aggaagaaag aagacaggtt
     1561 agcttgtgaa cgtctacagt gtgattttag acacttatcg tactatgaat gtctctatag
     1621 gaaagataga aatggcaatt ttctataccg tcatatctat agtgaattaa aaaatgagga
     1681 cacattaaag aatgatatca taaaagagct gttaatgcat ttagacgaca aatgtgtcgt
     1741 atattgtccg ctttcacttg gtgaccatgt tgatcatgtt tttgtgaata gtattggaag
     1801 ggcgcttgaa tttatgcgtt ataaagtaat atattatgag gacttcccat atgtaagtga
     1861 tagtagtatg gttagttata tgggaaaaac taaagaatta aaaatgtatc aagaggaact
     1921 tgatgaaaaa ca
//
LOCUS       DAVKGU010000154         1810 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVKGU010000154 DAVKGU010000000
VERSION     DAVKGU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.156"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(153..917)
                     /locus_tag="VW077_002061"
     CDS             complement(153..917)
                     /locus_tag="VW077_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9456003.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(910..1680)
                     /locus_tag="VW077_002062"
     CDS             complement(910..1680)
                     /locus_tag="VW077_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9456004.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGDGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFWGLTGTEKEDLHV"
BASE COUNT          626 a          400 c          295 g          489 t
ORIGIN      
        1 taaaacaatt tctaacaata tttaaaaact gaaccgtact atttcaactt ccataaatta
       61 caatattgat aggcacagtt cccatacctg tccaaattcg aataacgttt agtataaatt
      121 agaatatgac tacaacatca caaataatta gactatctat aatatctacg atgtaacata
      181 tacataggaa gcaccgttat caagagcgcc aaggctatca accagataag atgaagatac
      241 aaacctgtca cctcacctcc ccatataata cgaatcatgc gggtagcgtg atacaaggga
      301 gaaaaccaag tcaaatcacc aagtattggg ggcaaggaag taatgggaaa gaaggtatct
      361 gaaaacatat aaagaggacg gattaccaaa acattgtagt aattcagaaa ttccctagac
      421 ggcgccaagt aggtaaagat tagtcccaaa acaccaaaca aataacccac tataaataaa
      481 atcagaggaa tcaataacat caagacatga aaatgcgctg tgataagcat ggaaatcagc
      541 caaaaaatcc caccgaaaat cactgcacga atacctgccc agatagcctg acctaccaca
      601 atatcattcg tattgattgg cgtcatcaac atagcatcat aagtcttttc aatcttatac
      661 tggataaagg cattgaccgt tgtttctgcg gtggccgcag acatggcagt cgcagcaatt
      721 aaacctgtaa tgacaaaatc acggtagagc ataccgttaa catgcgtcaa ataagcacca
      781 actccaaatc ccattgctaa aagataaaag agggggtcga taaggttggg gacaattgaa
      841 attttaaata aatttctaaa actcatgtag ttgcgtacca caacgtgtcc gatggagtga
      901 aatgataact taaacatgca agtcctcctt ctctgtccca gtcaaccccc aaaaaagcgt
      961 ctcaaggttg tcagcatcat acttgatgag caagtcttct acagttcctt cttctttgat
     1021 agtcccttgg tggagcatca gcaccctatc tgtcaaatat tcaatctcat tcatatagtg
     1081 ggaagtaata atgatagaaa cacctaattc cttcaattcc aaaagtttat cccaaataat
     1141 attgcgggat tgaatgtcta aaccaactgt cggttcatct aaaatgataa tatcaggccg
     1201 attgatgaga gctcttgcca agacaagacg tcgtcgcatc ccacccgaca gctgattcac
     1261 catcttttgc gcatgtttgc tcagttgaac aaaatccaag agagaatcga ttctcttcct
     1321 aagttcgaca gatgagatac cataacatag accgtgagca aaaagattat cataaacatt
     1381 caaggtcatg tccagcatat cctcctgccc tacaataccc agacaagatt tactcttacg
     1441 acgattggtt tcaatatttt gccccaagat atcaatctct cctgaggaaa ttgaggtcag
     1501 tgagtaaatc atctttaaag tggatgtttt gccagcaccg ttgattccca agatacccaa
     1561 acactctccg tctcccaatt caaagctaat tcccttgaca gcttcataag aaccatattg
     1621 cttttttaga tttttaacac ttaacttctt cactctttct ttcccccaca aatagagcat
     1681 gttggatact ttgttaattc atctcacttt tgaaaaaatg ttgagaatgt aatcttaaga
     1741 ttaacgtcct tcaactgtgg atactgctgc aattactttt gtaattttac ctccatttaa
     1801 actcagacaa
//
LOCUS       DAVKGU010000155         1783 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVKGU010000155 DAVKGU010000000
VERSION     DAVKGU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.157"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            35..476
                     /locus_tag="VW077_002063"
                     /pseudo
     CDS             35..476
                     /locus_tag="VW077_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            641..940
                     /locus_tag="VW077_002064"
     CDS             641..940
                     /locus_tag="VW077_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9456005.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1201..1524
                     /gene="tnpB"
                     /locus_tag="VW077_002065"
                     /pseudo
     CDS             1201..1524
                     /gene="tnpB"
                     /locus_tag="VW077_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1661..>1783)
                     /locus_tag="VW077_002066"
     CDS             complement(1661..>1783)
                     /locus_tag="VW077_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9456006.1"
                     /translation="AFRTLEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFESR"
BASE COUNT          598 a          354 c          308 g          523 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaagcg aatacagact atattgctcg ctatggcgta tatgcagtta tccctaatcc
      121 tgaacaaaaa caaattgttc ttgttcaaga accaaatggt gcttggttcc taccatgtgg
      181 agaaattgaa gcaggtgaaa atcatcagga agccctaaag cgtgagttga ttgaagagct
      241 tggtttcaca gcagaaattg gtacctatta cggacaagct gacgaatatt tctattctcg
      301 tcatcgtgat acctactact ccaatcctgc ctacctctat gaagcaactc ctaaagccac
      361 tagaaaactt taatcatatt gcctggttcc ctattgacga ggctatcaaa aaccttaaac
      421 gtggtagcca taaatgggcc attgaatctt ggaaaaaaca gcataagatt gactaatact
      481 cttcgaaaat aaaattcaaa ccacgtcagc gtcgccttac cgtactcaag tatagcctgc
      541 ggctagcttc ctagtttgct cttttatttt cattgagtat aaatcaagcg actttattca
      601 aaaagtgcta taatagaatt agaaaatcgg aggaaatatt atgaagctta tcaatacgac
      661 aaactcacac tcgcaacttg taaaaagtca gctagaaagt accgatgcaa cccttgttga
      721 ggtttattct gcagggaata cagatgttat ttttacccaa gctccgcttc actatgaaat
      781 cctcatctca aacaaacacc gtgctattcg tgagcctgaa atcgaaacca ttcaagaatt
      841 tttcttgaaa cgtaaaattg ataagacctc tgttgatgaa gccaatatca agacactcta
      901 ctcagagaaa ttaattggaa tttcaatccc aatcaagtaa gaaaattgag taaaaactcg
      961 atttgagaga aagtccaata agtctttcca taacaaaacg catagtaaca agagtttcta
     1021 cacccactat tatacgtttt tatcattttg aaatctgctg ctaatcaatg aagaagcaaa
     1081 gatcaaacta ggcagctagt tacaagttac gtaagcgcgt cataacaagg tatctatcct
     1141 aattccccaa cttccagtat actagataaa aaataattat cagaggaaaa gtattgtact
     1201 atgatgattc tactaagtga tttaggtcaa gttcaccttg tttgtggcaa gacagatatg
     1261 aggcagggaa tagactcatt agcctatgta gttaaaaccc actttgaatt ggatcctttc
     1321 tcaggtcaag tttttctctt ttgtggtgga cgtaaagacc gctttaaagc cctttactgg
     1381 gatggtcaag gattttggct actatataaa cgctttgaga acggaaaact gacttgacca
     1441 agtacagaaa aggatgtcaa agctctcgca cctgaacaag tagattggct tatgaagggc
     1501 ttttctatca ctccaaaaat atagtagatt gaaactagaa tagtacacct ctgcttctaa
     1561 aacattgtta gaaatcgatt tgtcctgttc ttatttcatt ttactataaa tccatcagaa
     1621 agtcgtgatt tctattgaaa tgaggacttt ctttttatac tcatctgctt tcaaaaagca
     1681 ttctagtcca tctccgatta acgatggact ttatcacctc cttctccagt ccttgtatga
     1741 catcttggag ttgattcatg acatcttcca aagttcgaaa ggc
//
LOCUS       DAVKGU010000156         1755 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVKGU010000156 DAVKGU010000000
VERSION     DAVKGU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.158"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            17..>1755
                     /locus_tag="VW077_002067"
     CDS             17..>1755
                     /locus_tag="VW077_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEU9456007.1"
                     /translation="MANNRQIETLGVSYLTTFINRHSLLQTYFGSNDKTPVWDGEIHV
                     LKTSSEKRSEIFGKVPVQIKATRQQKNKLKSFSLDISDLELYSKNGGVVLFVVWLSED
                     GDLRNIYYKSLPPLSIKKLIKKSNLKNKTTSSKKLSVQIHELDEQKLYPMLVDFITNS
                     RKQYSFINVDGISVEDISDDSNLKFYYYGQEKGEIFNYQEENDLFIYYKNPLTGIEVP
                     LENTIKVVETYEETDLIITIGNTIFQNVKRHRFPDGSVQLHFGEGFKMSFDVKKKQFT
                     FNYTRPNMLSKAIKCTQAFQELGKFGYCKLNGNIIELDEQSILDITSRDLETEIEELV
                     QISNFMENMRIQKEVDLTYFDKQSLRNLNILNLGLILKNKVALNYNESKLLHLRIANI
                     HIITLYDFETDNIGTMIDIFTETPWCRRGEGEDSSYISIFEVLEPNDWLKIDNCDFDS
                     VISSYQRLVDNGLKFEDANNTIIKIVIAADKAEDVSRSELLLNWAQCLSDWNLKYSKN
                     CEMAIINDLQIKSRVRKLNSKEMEILSNILVNSNDNYELCFGSSVLLKSKPQADLFWN
                     KLDNETKESYKEFP"
BASE COUNT          666 a          232 c          302 g          555 t
ORIGIN      
        1 ttatcgaggt tagaatttgg caaataatag acaaattgag acattaggtg tttcctatct
       61 gacaacattc ataaatagac atagtttact tcagacttat tttggtagta atgataaaac
      121 tccagtctgg gatggtgaga ttcatgttct taaaacttct agtgagaaaa gaagtgaaat
      181 atttggtaaa gtgcctgttc aaattaaagc tacaaggcaa cagaagaata aattgaaatc
      241 cttctcgttg gacataagtg atttagaatt atattcaaaa aatggtggtg tagttttatt
      301 cgtagtttgg ttaagcgagg atggtgattt aagaaatatc tactataaat cattacctcc
      361 tctttctata aagaagctga ttaaaaaaag taatttaaaa aataaaacaa caagcagtaa
      421 gaaactttct gtacagattc atgaattaga cgaacaaaaa ctgtacccta tgctagtcga
      481 ttttataaca aatagtcgta agcaatatag cttcataaat gttgatggta tttcagttga
      541 agatatctct gatgatagta accttaaatt ctattattat ggtcaagaaa aaggagagat
      601 ctttaactat caggaagaaa atgatctctt catttattat aaaaatcctt taactggtat
      661 tgaagtacca cttgaaaata caataaaggt tgtcgaaact tacgaggaaa cagatctaat
      721 tattacaata gggaatacta tttttcaaaa tgtaaaaagg catagattcc ctgatgggag
      781 tgtacaactc cattttggtg aaggttttaa aatgtcattt gatgttaaga agaaacaatt
      841 cacatttaac tacacaagac ctaatatgtt atcaaaagcc ataaagtgca ctcaggcatt
      901 tcaagaacta ggaaaattcg gatattgcaa gctcaatggt aatataatag aactagatga
      961 acagtcaata ttggatatta cttcacgtga tttagagact gagatagagg aattggtaca
     1021 gatttcaaac ttcatggaaa atatgaggat acaaaaagag gttgatctaa cttactttga
     1081 taaacaatct ctaagaaatc taaatattct caatttaggc cttattctaa agaataaagt
     1141 tgccttaaat tataatgaat caaaattact tcacctaaga attgctaata tccacattat
     1201 taccttatat gactttgaga cagataatat tggaactatg atagacattt ttactgaaac
     1261 tccttggtgt aggagaggtg aaggtgaaga ttcgtcctac atttcaatct ttgaagtttt
     1321 agaaccaaat gactggctaa aaatagataa ttgtgatttt gattcggtta tctcctctta
     1381 ccaaagatta gttgataatg gcttgaaatt tgaagatgct aacaacacta ttataaaaat
     1441 agttattgct gctgataagg cagaagatgt ttccagaagt gagttattgc ttaattgggc
     1501 acaatgtctt tctgactgga acttaaaata ctcaaaaaat tgtgagatgg ctataataaa
     1561 tgaccttcaa ataaaatcta gagttcgtaa attaaacagc aaagaaatgg agattctgag
     1621 taacatttta gtaaatagta atgataacta tgaactttgt ttcggttcat cagtgttatt
     1681 aaaatcgaaa ccacaagctg atttgttctg gaataaactt gataatgaga caaaagaaag
     1741 ctataaagaa tttcc
//
LOCUS       DAVKGU010000157         1751 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVKGU010000157 DAVKGU010000000
VERSION     DAVKGU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.159"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            375..1247
                     /locus_tag="VW077_002068"
     CDS             375..1247
                     /locus_tag="VW077_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9456008.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHVTSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(1393..1737)
                     /locus_tag="VW077_002069"
                     /pseudo
     CDS             complement(1393..1737)
                     /locus_tag="VW077_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          572 a          313 c          331 g          535 t
ORIGIN      
        1 gtacgctgta gctgctaaaa catttctagg aattaatttg cctttcctaa tagagttgtt
       61 catatcttat ttcaatctac tatatttttc acctctaaca atcgcaatct cttctttatc
      121 catgaataaa aacgatttct atttttgtaa gtaaagcatc acacgaaatc cacgaaaatg
      181 aaaacctttg ttgtgttttc gtaaaaaatt tgttgacaga gcacgaaacg cctactataa
      241 ttaaatcaag tttaaggaaa taacaaaaaa acaaatcgga gggaaagacg atgaacacat
      301 taaatgagaa agtaatcaat atctgtaaag cagtagttaa agaaacttta atccaagaca
      361 tttaggagaa agaaatgaag aaatcaagaa aattagccac attaggaatc tgttcagctt
      421 tgtttttagg cttggctgct tgtcaacaac aacatgttac ttctgagggg acgaatcaaa
      481 ggcaaagcag ttcagcgaaa gttccatgga aagcttcata caccaaccta aacaaccagg
      541 taagtacaga agaggtcaaa tctctcttat cagctcactt ggatccaaat agtgttgatg
      601 cattttttaa tctcgttaat gactataata ccattgtcgg ctcaactggc ttatcaggag
      661 atttcacttc ctttactcac accgaatacg atgttgagaa aatcagtcat ctctggaatc
      721 aaaagaaggg cgatttcgtt gggaccaact gccgtatcaa tagttattgt cttttgaaaa
      781 attcagtcac cattccaaag cttgaaaaga atgaccaatt gcttttccta gataacgatg
      841 cgattgataa aggaaaggtc tttgattcac aagataagga agagtttgat attctatttt
      901 cgagagttcc aactgaggca actacagatg tcaaggttca cgctgaaaag atggaaacat
      961 tcttctcaca atttcaattc aatgaaaaag ctcgaatgct gtctgtagtc ttgcacgaca
     1021 atctggatgg cgagtatctg tttgtaggcc acgttggggt cttagtacct gctgatgacg
     1081 gtttcttatt tgtagagaaa ttgactttcg aagagcccta ccaagcgatt aaatttgcta
     1141 gtaaggaaga ttgctacaag tatttgggca ccaagtatgc ggattataca ggcgagggac
     1201 tggctaagcc ttttatcatg gataatgata agtgggttaa actttaatag tgttatgata
     1261 tgacagtaat cttaggattt ttatttctat ttttgatgac ttggtgggtt atttcaatcg
     1321 ttaatagcca gaaaccacgc aaaaatgaaa catttattgg ctatatccaa agatacgata
     1381 ttgatgggaa tggctatcaa tcattaccgt gtcctcagaa ctgagaggag ttcttgaaat
     1441 cgtaaaacta ctttgaacaa gagttacttc aacccattgg ctccgacgga ttaagttgct
     1501 ttcgtgaaca ccaaaatcag ccgcaatttc ttcataagtg cggtattctc gcacatattg
     1561 aagagtggcc ataagaaggt cttctaggct taatttaggt tttcgtcccc cttttgtgtg
     1621 tttaagttga taagcagttt ttaatacagc taacatctct tcaaaagtgg tgcgctgaac
     1681 accaacaaga catttaaatc gtgcatcagt tagttgctta cttgcttcat aattcataga
     1741 actactatac c
//
LOCUS       DAVKGU010000158         1737 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVKGU010000158 DAVKGU010000000
VERSION     DAVKGU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.160"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            73..864
                     /locus_tag="VW077_002070"
     CDS             73..864
                     /locus_tag="VW077_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9456009.1"
                     /translation="MNFALNKGEIVGLAGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     VGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQEIFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLRITMSGNIQSSEFFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            880..1608
                     /locus_tag="VW077_002071"
     CDS             880..1608
                     /locus_tag="VW077_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9456010.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLSLLISFIPPILLTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLGFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPVFVASNTMSISQ
                     YLVVITTIILLYFFTLKKSKKYSF"
BASE COUNT          584 a          222 c          286 g          645 t
ORIGIN      
        1 ttaatggagg aataaaaaat gtaagttata aatgtaagta agcattatgg ttattcaatc
       61 attctcaaag atataaattt tgcacttaac aagggtgaaa ttgttggtct agcagggaga
      121 aatggagttg gtaagagtac gttgatgaaa attcttgttc agaataatca accgacttca
      181 ggtaatatta taagcagtga taatgttggg tatttaatcg aagaaccaaa attattttta
      241 tctaaaacag gtttagagaa tttaaaatat ttgtcaaatt tatatggtgt tgactacaat
      301 caagaaatat ttagaagttt gatccaagag ttagatttga ctcagtctat taataaaaaa
      361 gtaaagacct attctttggg tacaaaacaa aaattagctt tgcttctaac tctcgttacg
      421 gaacctgata tattgatttt agatgaaccg actaatggtt tagatattga atcatcacaa
      481 atagttttag cggttctaaa aaatttagct ttacatgaaa atgtgggaat tttaatatcg
      541 agtcataaat tagaagacat tgaagaaatt tgtgagagag ttcttttctt ggagaacggg
      601 cttttgacat ttcaaaaagt aggaaaagat agtcataatt tcttgtttga gatagctttt
      661 tcatcagcta cagatagaga cattttcatt accaaacaag aatttgggga tattgttcag
      721 gaagagggac tgagaattac tatgtctggg aatattcaaa gtagtgagtt ttttaaattt
      781 tttaacgaaa actctattaa agtagttgat tttgaaacta aaaaagagac gcttaaagat
      841 atttacttaa atcgttcaaa ataaaggaag gttataatca tgaaattaaa taaattgaat
      901 tttcttaagg aaaatataag aaatttatat tcatcaggcg taatatatct cagtttgctt
      961 atctcgttta taccgccgat attgcttaca ttctttattc taaagactca agggacatcg
     1021 cttggtatta agcatatttc aaacttttat gctatgctcg gtatgttaat ggctgttata
     1081 catgctaatc gagtcattag tagagatttt tcccacaata cggtaagttt gttttataat
     1141 caacagaaga atcggatgat ttacgtcttg tctaattttc tatatgccat ctcagtttcc
     1201 attatttatg ctttgaatgg cattgtgtta ctagtcatcg taagtaaatt gggtattcca
     1261 ggtgatttag gattagattt tatagtagct attgtagtca atacaatttt gttagtcctg
     1321 ttttattttc tattatccta cattttctat ttatacaaat tgaaaagtgg cttgggattt
     1381 ggtattttag tagctttact actctttatc cctaatatat taaatacgat gatgatgaat
     1441 actagtaatg atttgtttat caaagcaatt gaacttcttc ctttttattc tttacctgta
     1501 tttgtggctt caaatacgat gtctattagt cagtatcttg tggtaatcac tacaatcatt
     1561 ttattgtact ttttcactct caagaaaagc aagaagtatt cattttagtt tcatttagta
     1621 ttattttgca tacctaaaat acaataaaaa aatcagtcat cttggtatgc tcctgctttc
     1681 actattcaac acgtttttga cttatactag gctcatttcc aaaagcatta tataata
//
LOCUS       DAVKGU010000159         1618 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVKGU010000159 DAVKGU010000000
VERSION     DAVKGU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.161"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..273
                     /locus_tag="VW077_002072"
     CDS             <1..273
                     /locus_tag="VW077_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456011.1"
                     /translation="YLLYKVGFLGIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQD
                     AVASKQIVVINEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            588..1565
                     /locus_tag="VW077_002073"
     CDS             588..1565
                     /locus_tag="VW077_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456012.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLILFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYIGIKHVSIRR"
BASE COUNT          549 a          211 c          271 g          587 t
ORIGIN      
        1 tatttactat ataaggttgg atttttggga atcatttacg ataatgaagc attaagagat
       61 ttgtcaaaac aatatttaaa caaaaatatt tcggaattac aagatggttc tttcgagggc
      121 ggtatacaag atgctgttgc ctctaaacaa atagtagtta taaatgaatt tatagcttgt
      181 ttaaattcca gatgcgtgcc atcccaatat gatagatttt tttataaaga tagaccttat
      241 attttttcca gaaagagtcc tattaagggg tgaaaatttg ttacatttgg attaacaagt
      301 gtcctaggga ttagaaataa ctctagtata gctagatatt tttgagtaac tagaaatatc
      361 attaggctac ccatatgatt gccagaaaag atggctgatg aatcttattt atactgttgc
      421 agtaggcatt gtctaggata aataagctat cttgatgggg gagattggaa taattttagt
      481 agaaacatta aaggattatg gacaagtagc gtaggattag gtgatgcaat gtgctaagtt
      541 ggcttggtgt tagcagtagt attatttaac aatggaaagt agactaaatg aaaaataagc
      601 gttatttttt tgacacaatt ttaataattt tactgcttat aagcacgata ttttgtgtca
      661 gcccagtatt tataaaattg gacatcttag gaactccctc tcatgccatc ttaacctttg
      721 tattggcgat accgttattc tatattttgt ctcaatgctt acatactttg ctattactag
      781 tttcctcaat cttttgtaaa ttaagaccaa tttattttta ttttatattt gtgatcatta
      841 taggagctag gaaatactat cgaattctct ttcaccaact aatgggattt agtccaggaa
      901 tagcagtgtt ttataaggag agtcaaacaa ctaagaatct gtttaaattt tactacttct
      961 tgtactttac aacactaatt tcatactatt ttttctttac ttttgtgtat gacaaaccgt
     1021 tgcttttgcc tcttatttta ttttctatta tcatagcatt agtacaaaaa ctttatagaa
     1081 ttgagaatca acaattattt ctgttaaaat caaaggtact aacaatactg gaatctaaga
     1141 agaattgtga gtttaatctt caggattatc atgaaatttg gaaattgcaa tcaaaatctg
     1201 agttgccatg cgtggcttta tcttatatca gtttaatcaa gccatatctt tcagagagtg
     1261 tgcgtgaaca aattgattta ttagaagtta agcgatttaa aaaaataaat catccaatat
     1321 cactttatgg tatgttggat gttataaaat taaatctata tttacggcat tataatgaaa
     1381 aaaataaata tgaatcaatg ttaaagaaaa ttttagaggt aagaccagac tttgttttaa
     1441 tcgagcagaa tattgatgat agtttaaata gctcgcaacc attatcttta tctttagcca
     1501 tttcagaaat ccaacttttg cttgaagtgt atatagggat caaacatgtt tcaattagga
     1561 gataaaacta gaataatatt tgccattata ggggcaagtt gcatattagt cagtatgt
//
LOCUS       DAVKGU010000160         1603 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVKGU010000160 DAVKGU010000000
VERSION     DAVKGU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.162"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>1603
                     /locus_tag="VW077_002074"
     CDS             <1..>1603
                     /locus_tag="VW077_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9456013.1"
                     /translation="KQVAPLPEYTGPQASAIVEPEQVAPLPEYTGVQAGSIVEPEKVE
                     APKEYTGKIEQPSAEDTKPENEASSTNGESERPKDKIKEEKQVDKKLELRNVSNVELY
                     TVENNKYRHITAVDGALDSSLKYFMKVKSENFKDIMLPVTKIESTTKNNKEVYKIVAH
                     AENLIQHENNVISNDYTYYLPKTQQSETGVYTSFKNLVDAMNSDPNGTFHLGATMDAR
                     EVELPDDQESYVKNEFYGKLIGENNGKYYAIYNLKKPLFKTLNTATIQNLSIKEANVS
                     SKEDAATISKEAKYNTLIDNVHSDGIIAGERGIGGLVSKVDNSRISNSSFTGRITNTY
                     DTTVGYEIGGLVGKLSGSLASIEKSIASIDIASNAKSGDQIVGGIAGVVEKSATIKYS
                     YVEGNVNNVRHFGKVGGVAGNLWDRDSQDVSKSGKLSYVLSDVNVTNGNAISGDNFDN
                     MKEDHAYSSKGNKVVNVVQVDDELVTKDSDVQRGTVLDADKVKEKKAELVSKHSTKVE
                     DFDFTSRYTTIYNEVTGYQKSREQVY"
BASE COUNT          629 a          216 c          322 g          436 t
ORIGIN      
        1 aaacaggtag caccgcttcc agaatataca ggaccacaag ccagcgctat tgttgaacca
       61 gagcaagtag caccactacc agaatacaca ggggtacaag ccggatcgat agtcgagcca
      121 gagaaagtag aagcaccgaa agaatatact ggtaaaatcg aacaacctag tgcagaagat
      181 actaaacctg aaaatgaggc aagctctaca aatggggaat cggaacgtcc taaagataaa
      241 attaaagaag aaaaacaagt cgacaagaaa ctagaactta gaaatgtttc aaacgtggaa
      301 ttatatacgg tagaaaataa taaatatcga catattacgg ctgtagatgg tgctttagat
      361 agttcattga agtatttcat gaaggtaaaa tcagaaaatt ttaaagatat aatgttgcct
      421 gttactaaaa ttgaaagtac aacaaaaaac aataaagaag tatacaaaat tgtggctcat
      481 gcagaaaatc taattcaaca cgaaaacaat gttatttcga atgactatac atattattta
      541 ccaaaaactc agcaaagtga aactggcgtc tatacatcat ttaaaaattt agttgatgca
      601 atgaatagtg accctaatgg aacattccat ttaggtgcaa ctatggatgc tcgtgaagtt
      661 gaacttccgg atgatcaaga aagttatgta aaaaatgaat tttacggaaa attaataggt
      721 gaaaataatg gtaagtatta tgctatttat aacttgaaaa aacctttatt taaaactcta
      781 aacaccgcta ctattcagaa tctttcaata aaagaagcga atgtttcttc taaagaagat
      841 gctgctacaa tatcgaaaga agcaaaatac aatactctta tagataatgt tcattctgat
      901 ggtattatag ctggtgaacg tggaattggt ggattggttt caaaagtaga taactcgaga
      961 atttctaata gtagttttac aggcagaata actaacacat atgatacaac tgtagggtat
     1021 gaaattggtg gtttagtagg taaactttct ggttcgcttg cttctataga gaaatcaata
     1081 gcatcgattg acatagcctc taatgcaaaa tcaggagatc aaattgttgg tggtatagcc
     1141 ggagttgttg agaaaagtgc gacaattaag tatagctatg ttgaaggaaa tgttaacaat
     1201 gtgcgacact ttggtaaagt tgggggagtt gcaggtaatc tttgggatag agattcacaa
     1261 gatgtaagta aatcaggtaa actatcatat gttttaagtg atgttaatgt aacaaatggt
     1321 aatgctatat cgggtgataa ttttgataat atgaaagaag atcatgctta tagtagtaaa
     1381 ggcaataaag tagtaaatgt agtccaagta gatgatgaac ttgtgactaa agattcagat
     1441 gtacaaagag gtacggtatt agatgctgat aaagtaaaag agaaaaaggc agaattggtt
     1501 tccaaacaca gcactaaagt agaagatttt gattttactt caagatacac tactatttac
     1561 aatgaagtaa caggttatca aaagtcgaga gaacaagtat ata
//
LOCUS       DAVKGU010000161         1549 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVKGU010000161 DAVKGU010000000
VERSION     DAVKGU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.163"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..731)
                     /locus_tag="VW077_002075"
     CDS             complement(<1..731)
                     /locus_tag="VW077_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456014.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFMFSYILLFVQ
                     SYLGSNTAWIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILIAI
                     FENLLDSYLLQNQSEVDLDIFYKVLP"
     gene            complement(888..1133)
                     /locus_tag="VW077_002076"
     CDS             complement(888..1133)
                     /locus_tag="VW077_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456015.1"
                     /translation="MGIYGRKTIAMKHFFAGIGEIHFLLFLLYLSMSASPIIGLVIGY
                     TKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
BASE COUNT          587 a          275 c          197 g          490 t
ORIGIN      
        1 ggtaaaactt tataaaaaat atctaagtca acctcactct gattctgaag caagtaggag
       61 tcaagcaaat tttcaaaaat agctatcaat atgattggtg taaggttttt gtattcacat
      121 gtcaagtatt ctgagtatcg tgcagatgat atttctttta cataactttt tgaaagcaag
      181 attttttcaa attcatcatc tattattaat cttctatttc caatccaagc ggtattgcta
      241 cccaaataag attgtacaaa tagcaatatg taagagaaca tgaacgtaaa tactgaaaga
      301 ggtagaattc tatgcgtaaa aaaatagacg ataatggtag ccaggagtaa agatatctct
      361 cgtgacaatc gaattctttt cagcatcttt tttaacagtc cgtaaggttg tccttggcaa
      421 tattcttgaa ctaaattaat cggaatgaca tctctcacca tttctggaaa gtagagtaag
      481 tttataggct gaaatctcca gctcccatcg taagtaaatg gatatagaac caaatactta
      541 aaagattttt ttcttataaa cttaattatc acaatatgaa tgactgttgc aatgaagtat
      601 attaaatata aaagtaaaac cgccatcaat atacttacaa aaatcccctt ggaatcaatc
      661 aatggaaaat aaaatattgc aactagaagc gaataaagtc ccgctaataa atatttctga
      721 tcatctttca ttcaaaacac catattattt actaagaata catcaataca atagagcaac
      781 tctgtaagtc aatatttgat aaagcaaagc tttaaaccct tccccagacg ttagctttta
      841 ttcaacccac tacagttgac aaggagccca aattattgat aaaaacttta gtcatcatcg
      901 tctctataaa aatattttct atacactctt gataatacca aaattaatgc caatatcata
      961 ccaagagcta taataataga atttattaga tttattttag tatatccaat cactagtcca
     1021 ataattggac tagcagacat gcttagatat aataaaaata acaaaaaatg aatttcacca
     1081 attcctgcaa aaaaatgttt catagctata gttttacgcc cataaatacc catcaaattt
     1141 accgtctacg atatctaaac catatgcaat ggctcctcct aatgagaaga cttcagcaac
     1201 agtagccgca atattaagag cattagtaat agttgccata ctaactccac caatatatct
     1261 tgttatagca cttagttacc tagataagat atcctttagc ttgcttccaa aatttaagtc
     1321 tacacctata atatgagttc atattagtac tgctaaatcc tatctatagt aatgtcaaac
     1381 acctaaatca agtcatctct ccaattcatc caagaaatga agaaaggcaa aaaatcttta
     1441 aaataagaaa agtgcatatt atcaggtgtt caaaaatctt gataatatgc gttatataat
     1501 agaaaaattt actcaatttc attttcaaat tgagtttttt cccagcctc
//
LOCUS       DAVKGU010000162         1480 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVKGU010000162 DAVKGU010000000
VERSION     DAVKGU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.164"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..388)
                     /locus_tag="VW077_002077"
     CDS             complement(<1..388)
                     /locus_tag="VW077_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HEU9456016.1"
                     /translation="MKGKSFLLKMLQNLKLIKRRLKPIKRKLKPIKRTYKKIVYSITK
                     SNMRKRLKNTDFSIISDNCWGGRVYEELGLPYRTPFIGLYIFSEDYVKLLKNFKKYME
                     YELTFTNNSKWNTEYDGEYPIGILNDI"
     gene            complement(389..1480)
                     /locus_tag="VW077_002078"
     CDS             complement(389..1480)
                     /locus_tag="VW077_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9456017.1"
                     /translation="MLHIPTGGLFSDGIGTFIYSYLEYMDLNKYDVTILATNPLLKSD
                     KEKFEYLGVKVIEIERKKSNLSKYIYKLKKLMKKEKFDIIHVHGSSALMSIELLTAKI
                     AGIPVRIAHSHNTTCEHHKLDKLLRPIFYRVYTQACACSVSAGQWLFEEKNYEIIHNA
                     RDVNKYQYDAIKRKKLREELLLSDETIALGHVGRFNTQKNQRFLVSLMERLVIKNIDL
                     KLFLVGTGDTLEEIKKLVIQKKLEDNIVFLGQFDDMKSFVSSMDIMLLPSLYEGLPLV
                     SVEWQINGVKSILSDRVTQECIYTETVQQLSIDSMELWEKEILNLSTSDRERVSLQNI
                     QLIRNAGYDISLEVKEIEELYNSLYDIAN"
BASE COUNT          474 a          270 c          174 g          562 t
ORIGIN      
        1 ctatatcatt gagaatacct ataggatact ccccgtcata ttctgtgttc cattttgaat
       61 tattggtaaa cgttagctca tattccatat actttttgaa gtttttcaac agcttcacat
      121 aatcctcaga aaaaatatac agacctataa atggtgttct atacggcaga cccaattctt
      181 catatacccg tcctccccag caattatcac tgataattga aaaatctgta tttttgagcc
      241 tcttcctcat atttgactta gtgatagaat aaactatttt cttataagtt ctcttaattg
      301 gttttaattt ccgttttatt ggttttaatc tccgttttat tagttttaaa ttttgtaaca
      361 tcttcaaaag aaaactcttt cctttcattc aattcgcaat atcataaagt gaattatata
      421 attcttctat ttctttaact tctaaagaaa tatcatagcc tgcatttctt attaactgaa
      481 tattttgcaa tgacactctt tctctatctg aagtagataa atttaaaatt tctttttccc
      541 ataattccat actatcgata gataattgtt gaacagtttc tgtatagata cactcttgtg
      601 taacacggtc agataggata ctttttactc cgtttatttg ccattcaaca gatactagtg
      661 gtaacccttc atataaactt ggaagtaaca ttatatccat tgatgacaca aaagatttca
      721 tatcatcaaa ttgacctaaa aaaacaatat tatcttctag ttttttctga atgactaatt
      781 tcttgatttc ttctaaagta tcacccgtcc ctactaaaaa taattttaaa tctatatttt
      841 tgatcactaa tctttccatc agagacacta gaaaacgttg atttttttga gtattaaaac
      901 gtccaacatg acctagagca atagtctcat cagacagtaa caattcttct ctaagttttt
      961 ttcgttttat ggcatcatac tgatacttat ttacatctct agcgttatgg ataatctcat
     1021 aatttttttc ctcaaaaagc cactgaccag cactaacact gcaagcacat gcctgagtat
     1081 aaactcgata aaatatcggt cgtagtagtt tatctagttt atgatgctca caagtagtgt
     1141 tatggctatg ggcaattcga actgggatac cagcaatttt agcagtcagt aattcaattg
     1201 acattaaagc gctacttccg tgaacatgta taatatcaaa tttttctttt ttcattaatt
     1261 ttttcaattt gtatatatat ttagaaaggt ttgatttttt cctttcaatt tcaataactt
     1321 taacccctaa atattcaaat ttttccttat cacttttcaa aagaggatta gtggctaaaa
     1381 ttgtcacatc atacttgttt aagtccatat actccaaata tgaataaata aaagttccta
     1441 taccatcaga aaataaacca cccgttggaa tatgtagtat
//
LOCUS       DAVKGU010000163         1460 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVKGU010000163 DAVKGU010000000
VERSION     DAVKGU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.165"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..559
                     /locus_tag="VW077_002079"
     CDS             <1..559
                     /locus_tag="VW077_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9456018.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHINALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="VW077_002080"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="VW077_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9456019.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          508 a          208 c          265 g          479 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgg
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcgggag ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattaacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtgattatg
//
LOCUS       DAVKGU010000164         1443 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVKGU010000164 DAVKGU010000000
VERSION     DAVKGU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.166"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            38..1039
                     /locus_tag="VW077_002081"
     CDS             38..1039
                     /locus_tag="VW077_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9456020.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT          491 a          170 c          290 g          492 t
ORIGIN      
        1 tatagtaata ttagaatgtt tgtaatagag gactgaaatg aaaaatataa atgggaaaaa
       61 ggtaactatt tatgatattg cacgtttatc aggtttttct ccaaaaacag tttcacgagt
      121 tattaatggt ggagtaaatg ttaaagaaga aacttatcaa gcgattcaaa aagttataga
      181 ggaattgtcg tatattccaa atgcgtatgc aaaaaattta actaaaaaag aagctataaa
      241 tatattgatt tctgtaaaaa aaatcgactc ttttccttta atttggtttc atacgctgtt
      301 agataaagtg ttacggacgt gtaaagaatt tggtgttaat gctatagttg agtactttgg
      361 agaggaggat acaattagta attcgattat atcaagcaca ggtagcctag tagatggtgt
      421 tattgttttt tatgaaagtg tagatgatat cagaattcag tatttaaaga agaaccatat
      481 gccttttctt gttttcggtg aatctcaaac gtctggagta gtctatgtat ctaataataa
      541 ttttcaagct acttatgata tgatgaaagc tgtaactgaa gaaaaattta aaaatatgtg
      601 gttgcttatg ggaggagaat ctcatgttaa taaggatcgt gaaagaggtg tgcgttcttt
      661 tctgaatgat aaaaattact ttatggattt aaaagtcatt tatggattat ctacaattga
      721 ttcagtttat tcttatgcaa tgcagcactt gactacccaa aattatccgg acattatttt
      781 tgtttcagga gatgaaaagg ttcagggctt gattcgtgcg tgctatgaga aaggaattct
      841 tattccagat gatatttcta ttattggatt tgataatatt cctatttctc agtattatac
      901 tccagctttg tctacaattg ctcccaatta tgttaaattg gctaaagaaa tgatagaagg
      961 tgttttagca attattaaag gggaaagtgt cacatctgtt gaagtttctc ctaaatttgt
     1021 taggagacag agtttctagt attgtttaat tcttgaaaat cctagtggaa aagatggagt
     1081 gttctaaaat aagttctgtt tagcctccat tcagtttcta ttgagttgga tccaatttct
     1141 aacaagtggg acatgttcat gttcgatcta aggaagacat ggtcacattc gaatgatatt
     1201 ttcaaaaaaa tcgtaaaaaa gtcttgacaa agaaaaaaca aagtattaaa ataagttcaa
     1261 caaatcagaa aagtaactat ttttgatctt cagggagcct gtggtggttg tgaataggtg
     1321 gttggaagta gtgaaagtgg gctgattttg aaaatgaatt tgaaataatg aaaattcggt
     1381 gcgcacacct tacagtgcag cttgttgtta aacaaggcag agatgtaaag ggatagtccc
     1441 ttt
//
LOCUS       DAVKGU010000165         1418 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVKGU010000165 DAVKGU010000000
VERSION     DAVKGU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.167"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(392..1285)
                     /locus_tag="VW077_002082"
     CDS             complement(392..1285)
                     /locus_tag="VW077_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9456021.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
BASE COUNT          478 a          288 c          157 g          495 t
ORIGIN      
        1 attatcaaat tacttttata tcactaagac tcaataaaag ataatgtcta tttaactttt
       61 atacgattac tacctccttt ttagtttaca cttcctacat cataaataaa catactaaac
      121 cctcacaatc tccaaataac tcttctatat cattgaaaat tgagtttcat cctctatact
      181 aatgtaggaa acctattaaa acattccttt aatccaatat aagaatacct tgttccatta
      241 atctctaccc gatattatag atagaaccaa attcctatac ttaaaaggac aaataacgtt
      301 ccaaccataa taaacctcct ttctttttga gaacgttttc tttataggtt tagtgtagca
      361 aaaacatgaa aaaaacaaca tgacttagaa gtcaggttgt tgggaaatct atctgttttt
      421 ctgctaaaat tctctgaact aatacctgat cgtctagcaa ttctgctaga gaaatcgcct
      481 tttcatagaa actttctgcc tctaaaatat tcttgtctac aaataaagca tattttgcct
      541 ttagcaaaaa tagatgaggt cggtaaaaaa catgtcttgt tttagcaata attttctcaa
      601 atgtttgaat atatatcaga ctatcttctg tctgaatttg gataaatagg ctcagaagaa
      661 ctttttccat ttgaactagg ctagccttat cttcatattc tgaacatagc aatacttttt
      721 gagctaactc ctcaaaatgt tctttatctt ctaatcccat agcacaacaa aagaaataca
      781 attcaataat caacaaatca tttacagaaa aatatttctt ttttaataac tgctggaaat
      841 attcttccac taaccctaaa ccgtaggtca catctgaact accataaata tcaaaacgag
      901 cttgtgtcac atccacaatt aactgctctt cctcaggcaa attctcataa tagttttcat
      961 atatttcctc aagtatagct tcttttcgtt cgattcttcc cttatcagcg taggtttgag
     1021 aatgaatcag ctcatctttg agttttagga atccttttgg aatctctata accttaacat
     1081 ccacaatatt ctgtaatgat actccaagtt tatccgcaat caataataat ttaggaagtg
     1141 ttgctaaagc ttgcccattt tcaatacgtg ctagctgacg aatcgtcaac tctgattcat
     1201 caccacaaat atctatctga gtttttccac aagatcgcct aatagttttg attttataac
     1261 caatttcacg tttcaactca tccatagtaa tactccttaa gttgattaca aagatttctt
     1321 tttcttccta attcttttgt caatcatata taaaacgata agcgaaaaca gcggtagaac
     1381 aatataactc ttttcactca ttatcagctg aaatccca
//
LOCUS       DAVKGU010000166         1413 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVKGU010000166 DAVKGU010000000
VERSION     DAVKGU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.168"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            87..1265
                     /locus_tag="VW077_002083"
     CDS             87..1265
                     /locus_tag="VW077_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9456022.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          520 a          258 c          203 g          432 t
ORIGIN      
        1 aaagcttaaa aagattaaca tctacattta tccatagcaa aaaatcatag gattttaaga
       61 atctgacaac taggaagaaa actaatatga ccttatctaa gaaacaatta caactaagag
      121 ctaaaatact ggaaacagtt tacacattag gtcccatttc acgaattgaa atcgctacta
      181 aaacaggaat aacacctgca acaactagca gtatcacaaa tgatctgatt aaagaaaata
      241 ttctcttaga acttggtgaa gacgaacacg atacaagcgt agggagaaaa aaaatcctgc
      301 tagacattca agctaaacga ttttactaca ttggttgtga actatctgaa aaacatttca
      361 cttttgcttt aggagataat ctaggaaata tcttaaagga agagaaagaa attgttacta
      421 aacagttaat ccaagaaaag ggaaatcaac tcattaacca gacactaaag caattcttaa
      481 ataactgctc tgattatgaa atagaagcaa ttggaatcgc gctacctggt cgctatttag
      541 ataattataa aataacaaca aacaatcctt tatggcaaca tatcgactta gaaatgattc
      601 aatcccactt tgataagcct ttgtttttct ctaacaatgt taactgtatg gctataggta
      661 aacgcctatt tagtcgccaa cagaatgacc ctaattttgc ttattttcat tttgctagag
      721 gtatgcactg ttcttatatc tacgacggaa acatttatgg gaaaggaaat ctaatgattg
      781 gagaaatagg gcatacagtt gtttcatctg agggagaaga atgtagctgc gggcgcaagg
      841 gatgcttaca aacttttgct ggagaatctt ggttaatcaa aaaatcaaaa attctatatc
      901 atcaatctcc ctattctcta cttcctagtt tagtcaaaaa tgccgatgat attgatatcc
      961 aagttatttt aaccgcttat caattaggtg acactggaat aatcacactt atccatcaag
     1021 ccttactcta tcttagtcaa accattttaa atatcagtat gatgattgac tctcaaaaaa
     1081 tctacctaca tagtccttta ctaacaaatc aacacatcat ccaaaaacta tatagtgaaa
     1141 tgaactacaa acccaaactg ctctataatc gcctacctga agtcattatt gaaccttaca
     1201 atgattttac agccgcacac tcagctattg cactttgttt gtatcataca attctacatt
     1261 cttaattttc tattacattt aataaatagg ctaaaaagag ggagtatatc ataaaaagcg
     1321 aataaacctg ttttctgaaa atcagaattc tgttttgttc gctttcttta tatttggaat
     1381 tatactccta tctccccttt ttcataatgt ata
//
LOCUS       DAVKGU010000167         1409 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVKGU010000167 DAVKGU010000000
VERSION     DAVKGU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.169"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            28..>1409
                     /locus_tag="VW077_002084"
     CDS             28..>1409
                     /locus_tag="VW077_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9456023.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSESEVSNQKSSVATSTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPAVQPELPEAVVTDKG
                     ETEVQPESPDT"
BASE COUNT          524 a          225 c          284 g          376 t
ORIGIN      
        1 atataaattt tatttcaagg aggaataatg gaaaagtatt ttggtgaaaa acaagagcgt
       61 ttttcattta gaaaattatc agtaggactt gtatctgcaa cgatttcaag tttatttttt
      121 atgtctgtat tagctagttc atctgtggat gctcaagaaa ctgcgggagt tcactataaa
      181 tatgtggcag attcagagct atcatcagaa gaaaagaagc agcttgtcta tgatattccg
      241 acatacgtgg agaatgatga tgaaacttat tatcttgttt ataagttaaa ttctcaaaat
      301 caactggcgg aattaccaaa tactggaagc aagaatgaga ggcaagccct agttgctggt
      361 gctagcttag ctgctctggg aattttaatt tttgctgttt ccaagaaaaa ggttaagaat
      421 aaaacggtat tacatttagt attggttgca ggaataggaa atggtgtctt agtttcagtc
      481 catgctttag aaaatcatct tttgctaaat tacaatacgg actatgaatt gacctctgga
      541 gaaaaattac ctcttcctaa agagatttca ggttacactt atattggata tatcaaagag
      601 ggaaaaacga cttctgagtc tgaagtaagt aatcaaaaga gttcagttgc cacttctaca
      661 aaacaacaaa aggtggatta taatgttaca ccaaattttg tagaccatcc atcaacagta
      721 caagctattc aggaacaaac acctgtttct tcaactaagc cgacagaagt tcaagtagtt
      781 gaaaaacctt tctctactga attaatcaat ccaagaaaag aagagaaaca atcttcagat
      841 tctcaagaac aattagccga acataagaat ctagaaacga agaaagagga gaagatttct
      901 ccaaaagaaa agactggggt aaatacatta aatccacagg atgaagtttt atcaggtcaa
      961 ttgaacaaac ctgaactctt atatcgtgag gaaactatag agacaaaaat agattttcaa
     1021 gaagaaattc aagaaaatcc tgatttagct gaaggaactg taagagtaaa acaagaaggt
     1081 aaattaggta agaaagttga aatcgtcaga atattctctg taaacaagga agaagtttcg
     1141 cgagaaattg tttcaacttc aacgactgcg cctagtccaa gaatagtcga aaaaggtact
     1201 aaaaaaactc aagttataaa ggaacaacct gagactggtg tagaacataa ggacgtacag
     1261 tctggagcta ttgttgaacc cgcaattcag cctgagttgc ccgaagctgt agtaagtgat
     1321 aaaggcgaac cagcagtcca accagagcta ccggaagcgg ttgtaactga caaaggtgag
     1381 actgaggttc aaccagagtc gccagatac
//
LOCUS       DAVKGU010000168         1373 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVKGU010000168 DAVKGU010000000
VERSION     DAVKGU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.170"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>1373
                     /gene="pcpA"
                     /locus_tag="VW077_002085"
     CDS             <1..>1373
                     /gene="pcpA"
                     /locus_tag="VW077_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9456024.1"
                     /translation="AVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKYKVLTV
                     EGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYYPDETG
                     RIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELEEIELP
                     ASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKTLGSNL
                     FRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKETKELASY
                     SFNKNSYLKKLELNDGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLELKELI
                     LPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKSTEFSVK
                     KDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKNSNQGV
                     VGWVKDKG"
BASE COUNT          531 a          190 c          228 g          424 t
ORIGIN      
        1 gcggttattt tagcagcata tgtccctaat gaaccaatcc tagcagatac tcctagttcg
       61 gaagtaatca aagagactaa agttggaagt attattcaac aaaataatat caaatataag
      121 gttctaactg tagaaggtaa cataggaact gttcaagtgg gtaatggagt tactcctgta
      181 gagtttgaag ctggtcaaga tggaaaacca ttcacgattc ctacaaaaat cacagtaggt
      241 gataaagtat ttaccgttac tgaagtagct agtcaagctt ttagttatta tccagatgaa
      301 acaggtagaa ttgtctacta tcctagctct attactatcc catcaagcat aaaaaaaata
      361 caaaaaaaag gcttccatgg aagtaaagct aaaactatta tttttgacaa aggcagtcag
      421 ctggagaaaa ttgaagatag agcttttgat ttttctgaat tagaagagat tgaattgcct
      481 gcatctctag aatatattgg aacaagtgca ttttctttta gtcaaaaatt gaaaaagcta
      541 accttttcct caagttcaaa attagaatta atatcacatg aggcttttgc taatttatca
      601 aatttagaga aactaacatt accaaaatcg gttaaaacat taggaagtaa tctatttaga
      661 ctcactacta gcttaaaaca tgttgatgtt gaagaaggaa atgaatcgtt tgcctcagtt
      721 gatggtgttt tgttttcaaa agataaaact caattaattt attatccaag tcaaaaaaat
      781 gacgaaagtt ataaaacgcc taaggagaca aaagaacttg catcatattc gtttaataaa
      841 aattcttact tgaaaaaact cgaattgaat gatggtttag aaaaaatcgg tacttttgca
      901 tttgcagatg cgattaaact tgaagaaatt agcttaccaa atagtttaga aactattgaa
      961 cgtttagcct tttacggtaa tttagaatta aaagaactta tattaccaga taatgttaaa
     1021 aattttggta aacacgttat gaacggttta ccaaaattaa aaagtttaac aattggtaat
     1081 aatatcaact cattgccgtc cttcttccta agtggcgtct tagattcatt aaaggaaatt
     1141 catattaaga ataaaagtac agagttttct gtgaaaaaag atacatttgc aattcctgaa
     1201 actgttaagt tctatgtaac atcagaacat ataaaagatg ttcttaaatc aaatttatct
     1261 actagtaatg atatcattgt tgaaaaagta gataatataa aacaagaaac tgatgtagct
     1321 aaacctaaaa agaattctaa tcagggagta gttggttggg ttaaagacaa agg
//
LOCUS       DAVKGU010000169         1308 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVKGU010000169 DAVKGU010000000
VERSION     DAVKGU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.171"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(700..1272)
                     /locus_tag="VW077_002086"
     CDS             complement(700..1272)
                     /locus_tag="VW077_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456025.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNRRINF"
BASE COUNT          396 a          222 c          218 g          472 t
ORIGIN      
        1 gaaggtgttt ttatgatata cacacttaaa ttggtgttgt ttattacctt tcttgtaata
       61 agcttgttac ctgataagat ttttggaaaa aataaaaaaa tttggaaaat agtttttgca
      121 atattgacgg cagtggcagc attgtcattt atgtactaag ttattttaag aatgtaggga
      181 aataaaccct acattctttt tagttttttc tgttttctaa attctattta tccaagcgct
      241 tcaacatttc ttgcttcttc gcttcaagtt ctgcacgctt ttcttcgatt tcggcatgtt
      301 ttttctcgag ttcagaacaa cttgcatcat tgctaaattc ttttcgccat caggagatag
      361 ggtgagtcga catgtctatt actcacccaa agcagtccta caaagcagga attttctgtt
      421 acttttttgg aaatagtaac gtttatacag ctttgacact tcgtatcaaa gcgccaaaca
      481 cactccgagg gatttacaga aagcagaaaa ggaatgatct ggtataagat cattcctttt
      541 ctctcttttt ctttaagtaa ttatatacaa tgtacgacga agtcgtcatt gcaatgctga
      601 tccaccacct aaagggaact ttaaacaaga ttgataagat aaagaatata aacaacgaaa
      661 atacgttata cccaattaat tttattgtat atctcatgat taaaagttaa tccttctgtt
      721 gttaggaatg gcatcatttt tatcccataa ttgtgctaaa taagtccccg gtgataataa
      781 attcatagcg aattctaaag caacatcatt tacaaaccaa ctacctagat atctagaaat
      841 tgctgaacga atagcacttt ttgctgcatg ttttcctttt actttaatta gatttgcaag
      901 gcctgcagta gttcctccta atgctaaagc tattgcagta tctaatagag cacccatttg
      961 attaactgta ataccttgcc aagctgctct aaatggagag tatgtaggtg ggattgtata
     1021 atcgccttgt aattgtcggt taattacttc tttgatccat tgttgtgaga cgtctggatg
     1081 aaaagattgg atttcgtttg caagtgtatt gatttgttct tctgttagag aagtgacagg
     1141 ttgaagttcc atatttgttt caatttgtga tacttgttca gaagcgtata cagctgaaac
     1201 acttggaatc gctgatacaa ttaacacaat tgacgtcaaa aaaaccgaaa taaatttcat
     1261 taatttgttc atgaactttt ctccttttta tttgtatctg cttacatt
//
LOCUS       DAVKGU010000170         1288 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVKGU010000170 DAVKGU010000000
VERSION     DAVKGU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.172"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(1..516)
                     /locus_tag="VW077_002087"
                     /pseudo
     CDS             complement(1..516)
                     /locus_tag="VW077_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(609..746)
                     /locus_tag="VW077_002088"
     CDS             complement(609..746)
                     /locus_tag="VW077_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456026.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEVLNIRLEVK
                     N"
     gene            1004..1123
                     /locus_tag="VW077_002089"
     CDS             1004..1123
                     /locus_tag="VW077_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9456027.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT          426 a          198 c          206 g          458 t
ORIGIN      
        1 tattcgttgt tgttcggata gtggggggag agcaattaat aatagattaa aattataatc
       61 attgattgca ggataacttg ttccagtaga tttattatta acacgattga taaaattatc
      121 tgataataaa taatatttca aatatgtttc gttaagtaaa gtatccaaaa caataaatgc
      181 tgtactagct atcaaatact ctttaagttc tctaactaca gcaatatttt ttagatatgg
      241 tctaactgtt gaaaataaga cactattctg cgaaactaat tttctagcac gggaaggcgc
      301 ttgttcaggt gaaagatatt gtagattttt gtagttgatt atgttctttt ttctatcaat
      361 actagacgta tctatatacc taaaggattt ctctggctta ttttgcccaa aattccaata
      421 aattgatttt atcctcaccc actcccaagt atcaggaata tcataaggaa catcaatttc
      481 ttgagtactt ccatcagcaa acttcccata atgtttctta tgtgcttcaa gtatataaaa
      541 aggcgtaaaa atacgcctat agataatggg gttgaaatag gtttattgtt gatgagattg
      601 tagataattc aattttttac ttccaatcga atattcaaaa cctccacctt ttctgcctgt
      661 aattgttcat cataaaattc aatatcttca ggattttccc cttggcaacc tcggcagaaa
      721 tattcttccg ctcgatcagg attcaaaaat cgacaagcac aaacaaaaca gtcgccatca
      781 tcatttattg agataatata gtagattgaa ataagatgta aacaaatcga ttaggaaagt
      841 taaattagtt tctagaaatt tttagcagat gtagtgtact attctagtct caatttacta
      901 tggcttcaaa tatatctttc gaaaaaatat ttacagatgt gtaattttga agcttgcaaa
      961 agttagtaaa cttgtagatt tcgatttgaa gtaacttgtt ttcttgcccg atattgtttt
     1021 tgaaattgaa tttttccata gtgactcctt gattttcttc tacacgtctg atgataaatc
     1081 taattcgcaa aagagtcaag aggatttttc gaaaaataaa tagcgaccga aatcgctatt
     1141 ttaagggtta taggtatttg atggcttaga ctgctgtgtg actgtttacc cacaggcaat
     1201 ctttcttggc tctataatat ttgtagtggg taaatccact atagatatta tggagcctat
     1261 tttattgtag aaaaaaagtc ccataaga
//
LOCUS       DAVKGU010000171         1287 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVKGU010000171 DAVKGU010000000
VERSION     DAVKGU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.173"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..329
                     /locus_tag="VW077_002090"
     CDS             <1..329
                     /locus_tag="VW077_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9456028.1"
                     /translation="EEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAG
                     PIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIK
                     MNRRNI"
     gene            329..1123
                     /locus_tag="VW077_002091"
     CDS             329..1123
                     /locus_tag="VW077_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9456029.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHINS"
BASE COUNT          476 a          208 c          214 g          389 t
ORIGIN      
        1 gtgaagaaat agcaatcgga cttggagcaa atcccgagct ttcaaggctt gttttaattt
       61 tttgcgctgt atctttaact gctttttcta cttcaattac aggaccaata gcttgtatat
      121 cttttttagc tggtcccata gccttaaata ttggcaagaa aagaagccca atattagctg
      181 gattggttgg aattttacta gttttgttat cagacatatt ctctcaaaat attttaccag
      241 ctagatatcc agtaggtgtt gtaactggct tgttaggttc accatactta atatacttac
      301 taataaaaat gaacaggaga aatatataat gaaaggtctg tggtcaaata atcttacctg
      361 cggttatgat gaaaaaataa tcttggaaaa tataaatata aaaatacctg aagaaaaaat
      421 atcagttatt attgggtcaa atggttgtgg gaaatcaaca ctcattaaaa ccttgtctcg
      481 acttataaag ccattagagg gagaagtatt gcttgataat aaatcaatta attcttataa
      541 agaaaaagat ttagcaaaac acatagctat attacctcaa tctccaataa tccctgaatc
      601 aataacagta gctgatcttg taagccgtgg tcgtttcccc tacagaaagc cttttaagag
      661 tcttggaaaa gatgaccttg aaataataaa cagatcaatg gttaaggcca atgttgaaga
      721 tctagcaaat aacctagttg aagaactttc tgggggtcaa aggcaaagag tatggatagc
      781 tctagcccta gcccaagata caagtatcct acttttagat gagccaacta cttacttgga
      841 tatctcatat caaatagaac tattagacct cttgactgat ctaaaccaaa aatataagac
      901 aaccatttgc atgattttgc acgatataaa tctaacagca agatacgctg attacctatt
      961 tgcaattaaa gaaggtaaac ttgttgcaga gggaaagcct gaagatatac taaatgataa
     1021 actagttaaa gatatcttta atcttgaagc aaaaattata cgtgacccta tttccaattc
     1081 gcctctaatg attcctattg gcaagcacca tattaactct taataattga atattggaat
     1141 tcatttaaaa gtcgcaaatt ttgaactgac cctcaaaagt taggccaaaa aactaactaa
     1201 aggaggtcag ttttttaatg gcaaatacag cacagaattt aagataaaat tagtaaaaga
     1261 atatcatgaa tgaaaaatag gatatag
//
LOCUS       DAVKGU010000172         1242 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVKGU010000172 DAVKGU010000000
VERSION     DAVKGU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.174"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..116
                     /locus_tag="VW077_002092"
     CDS             <1..116
                     /locus_tag="VW077_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9456030.1"
                     /translation="EYKDQISSTVLPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            1078..1239
                     /locus_tag="VW077_002093"
     CDS             1078..1239
                     /locus_tag="VW077_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456031.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
BASE COUNT          520 a          158 c          229 g          335 t
ORIGIN      
        1 ctgaatataa agatcaaata tctagcacag tattgccaga tatcaaaatt aaattaccga
       61 tagacagtag aggtactcca gattggaatt acatggaaag atatagagat agataaagag
      121 agagggagag atagataaaa agtccacccc cattttttat ggagaattca aaaatgtaag
      181 gttaagcaaa gaagaatata aaaatctaaa agaaaaatta aactcacaca cagatataat
      241 gataaataaa ctatccagat acatggaaag cagtggtaag acctatcaaa accactatgt
      301 gacaatctta aaatggtatg aagaagataa agacaaacta agacagaaag gtttaaataa
      361 aaaaatgaat tatgatgtag gagaatcttt atagataaaa agaggtggaa aggcactatt
      421 aaagacttta agtctaaaaa ggtatcaaag tatgccagag ataaaaaaat ataaatatga
      481 cactttaaag gcgattagaa aattaaagaa atgctagaag acttttagat gacagttacc
      541 aagagggtct aattttcact gctacttatg cagtgggaat ttgacctttt tatataagca
      601 aattctattt caaaattggt taccaagaaa gacttctttt cactgttaag tataagaggg
      661 taatttatag aattttatta cattaagata aatattttct gatggtcaaa ttttaaaaag
      721 attaataact ctcgataaat tttgaacctt aacaattgaa tagaccaaga caagacgtta
      781 attatttttg gagcgtaata acctatccgt caagatcttt cggctgaata attcggcaaa
      841 aaaagttctg ctaagtaaaa agaaagtgag cgataagtaa gagtttgaat atagggaggg
      901 ataccctatc cgctatgaag aaaatgagga taatgatact tctaaggttg aagccggctc
      961 atccggaata gaggcggagg tgagattcct atgttgctaa tccaaagcac ttgtcaatgt
     1021 caaaaaactt aattaaacaa ataattatta gatataaaac tttagaggag gtatatattg
     1081 gacaatgact ggaatggatt ggctgattta atagcaaatc tgattgcaaa atatgctggt
     1141 gctttagatt tagataatct acccgatcca acgccagcaa aaaatcaaga gatgaaaaat
     1201 agttttgaca tggcaaaaac acaaattgag acggactaat aa
//
LOCUS       DAVKGU010000173         1219 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVKGU010000173 DAVKGU010000000
VERSION     DAVKGU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.175"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(87..803)
                     /locus_tag="VW077_002094"
     CDS             complement(87..803)
                     /locus_tag="VW077_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9456032.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(807..1118)
                     /locus_tag="VW077_002095"
     CDS             complement(807..1118)
                     /locus_tag="VW077_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456033.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
BASE COUNT          364 a          234 c          176 g          445 t
ORIGIN      
        1 aaaaagagcc tgttcgtttt ttggttatat gaaaattagt gacttgtaga ttttcatctt
       61 ataccatttc cagcaataca agtagctcat agaaaataag cgagccactc attcattaga
      121 ctagcgattt ctttaggtgc ttgagtataa agctcatggc caaagttttc taaaaaaata
      181 gtatcaaaat agtctggcaa ttcttttagg gcttcctctc tccatgtagc ttcattagga
      241 tagcgaggac taataaacaa ggtatctccc acttctctct taaaagcttg tatttttctc
      301 cgtaggcgga gtatcgcttc tatattttca taatttatag ccaactcata tctattatac
      361 tcaacattcc agtgatagga ctgtcttaca gctttctcca tattttctga ccaatgcttt
      421 gcttcagatt tttctttaga agtaagaaca tctaagtccg aaataatttg agatttgata
      481 taatttttag tttcctctaa ctctgtatcc aaaggtaaaa tcttatctaa atctagatag
      541 ccaccatcca aaagaatcag tttctttact tcttcaaatt ccgatgcgaa ataacgagct
      601 aaatcgcctc caatagaatg acctatcaga cagatagatt cttcctctac aatctcattt
      661 ttaaaccatg atttcaattc tgtttcatct cgaagatgct tttcatatgg atttagaaaa
      721 tagacctgcg aatctagttc ttgaagaaaa tccttgctat gataggcatt gcttcccaaa
      781 ccgccaataa aatatttttt cattctctac ttaatactat gcttattcat cttttgttca
      841 aagatagttg tgataatctg acgcaattct tcgcgttctt tttctggaat ctcaccactt
      901 gtttgagcta cagcgtagag ttcagggtgt tcaattgaaa tgcgtttaat cgtacgtgtt
      961 gtagcatgtt ttctgacaaa aaacgggatt cgcttaatca agtcttgtgg tactagcgcc
     1021 agaatcttct cagtagtttc tttgtcacta atattagaca ttgtaagcct tttcttaatc
     1081 atttcctgtt ctttttctgt aaaatctttt aattccattc gattagtcct cctattttct
     1141 ctaagttaaa ttatatacta atacagatga aactacaaag aataaacttt aagaaatctt
     1201 ctcactgata agattttag
//
LOCUS       DAVKGU010000174         1186 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVKGU010000174 DAVKGU010000000
VERSION     DAVKGU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.176"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>1186
                     /gene="phtD"
                     /locus_tag="VW077_002096"
     CDS             <1..>1186
                     /gene="phtD"
                     /locus_tag="VW077_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU9456034.1"
                     /translation="LIFDPAQITSRTARGVAVPHGNHYHFIPYSQMSELEERIARIIP
                     LRYRSNHWVPDSRPEQPSPQSTPEPSPSPQSAPNPQPAPSNPIDEKLVKEAVRKVGDG
                     YVFEKNGVSRYIPAKNLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAY
                     DLLARIHQDLLDNKGRQVDFEALDNLLERLKDVPSDKVKLVDDILAFLAPIRHPERLG
                     KPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDS
                     LSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNL
                     QYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERP
                     HS"
BASE COUNT          379 a          262 c          246 g          299 t
ORIGIN      
        1 gccttatttt cgacccagcg caaatcacaa gtcgaaccgc cagaggtgta gctgtccctc
       61 atggtaacca ttaccacttt atcccttact ctcaaatgtc tgaattggaa gaacgaattg
      121 ctcgtattat tccccttcgt tatcgttcaa accattgggt accagattca agaccagaac
      181 aaccaagtcc acaatcgact ccagaaccta gtccaagtcc gcaatctgca ccaaatcctc
      241 aaccagctcc aagcaatcca attgatgaga aattggtcaa agaagctgtt cgaaaagtag
      301 gcgatggtta tgtctttgag aagaatggag tttctcgtta tatcccagcc aagaatcttt
      361 cagcagaaac agcagcaggc attgatagca aactggccaa gcaggaaagt ttatctcata
      421 agctaggagc taagaaaact gacctcccat ctagtgatcg agaattttac aataaggctt
      481 atgacttact agcaagaatt caccaagatt tacttgataa taaaggtcga caagttgatt
      541 ttgaggcttt ggataacctg ttggaacgac tcaaggatgt cccaagtgat aaagtcaagt
      601 tagtggatga tattcttgcc ttcttagctc cgattcgtca tccagaacgt ttaggaaaac
      661 caaatgcgca aattacctac actgatgatg agattcaagt agccaagttg gcaggcaagt
      721 acacaacaga agacggttat atctttgatc ctcgtgatat aaccagtgat gagggggatg
      781 cctatgtaac tccacatatg acccatagcc actggattaa aaaagatagt ttgtctgaag
      841 ctgagagagc ggcagcccag gcttatgcta aagagaaagg tttgacccct ccttcgacag
      901 accatcagga ttcaggaaat actgaggcaa aaggagcaga agctatctac aaccgcgtga
      961 aagcagctaa gaaggtgcca cttgatcgta tgccttacaa tcttcaatat actgtagaag
     1021 tcaaaaacgg tagtttaatc atacctcatt atgaccatta ccataacatc aaatttgagt
     1081 ggtttgacga aggcctttat gaggcaccta aggggtatac tcttgaggat cttttggcga
     1141 ctgtcaagta ctatgtcgaa catccaaacg aacgtccgca ttcaga
//
LOCUS       DAVKGU010000175         1173 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVKGU010000175 DAVKGU010000000
VERSION     DAVKGU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.177"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..838
                     /locus_tag="VW077_002097"
     CDS             <1..838
                     /locus_tag="VW077_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEU9456035.1"
                     /translation="DMLRKYRKDQKHFYLTHGCFPLKNTIGKITVPNYVDFVLSPSGN
                     VDNIIVKQFGLTSKEQCLHTGFPCHDIFYSKTEPLVFLSDYDKKIIWAPTFRKGGGYN
                     RNDSTASYPLGIPLLGTLDELAHLNTYLSQRDIVLIVKLHPMQDISDLELKQFSHIKF
                     LTNQNLKRKNINMYQLLMNSDALLSDYSAISYDYLHLDKPIGYVFSDLNDYKLGFSVD
                     NVDDYIAGDKIMSFNDLLHFIDNIYLEIDKNKEKRKELFNKIFEKQDGESCERLAQIM
                     EL"
     gene            855..>1173
                     /locus_tag="VW077_002098"
     CDS             855..>1173
                     /locus_tag="VW077_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU9456036.1"
                     /translation="MSVGMKAALWYTVANFIQKGFVFLSTPILTRILNVNDYGTLILY
                     QSWFVLFAIFATLNLSQTAYNKGLVEFENDKDNFTFSLLFLSKIITIIVAFLYFTFNI
                     LVKD"
BASE COUNT          402 a          154 c          195 g          422 t
ORIGIN      
        1 tgatatgctt cgtaaatata gaaaagatca aaaacacttt tatttaacac atggatgctt
       61 tcctttaaaa aatacaatag gtaaaataac agttccgaat tatgttgatt ttgttctatc
      121 tccatcgggc aatgtagata atattatagt taagcagttt ggattaacat ctaaagagca
      181 atgtcttcac actggatttc cgtgtcatga tatcttttat tcaaaaacag aacctctagt
      241 atttttatct gattatgata aaaaaattat ctgggcacca acttttagaa agggtggtgg
      301 ctataataga aatgatagta cagctagcta tcctttaggt attccattac ttggaacact
      361 agatgaactt gcacacctaa acacatatct aagccaacgg gatattgtcc tgattgtaaa
      421 gttacatcca atgcaagata tttcagattt ggagttgaaa caatttagtc atattaagtt
      481 tctgacaaat caaaatctga aacgtaaaaa tataaatatg tatcaattgc tgatgaattc
      541 agatgcttta cttagtgact attctgcaat ctcgtatgat tatttacatc ttgataaacc
      601 aatagggtat gttttttcag atctaaatga ttataagttg gggttttcag ttgataatgt
      661 agatgattac atagcaggtg ataagataat gtcttttaac gacttgctgc actttattga
      721 taacatttat ctagaaattg ataaaaataa agaaaaacgc aaggaattat ttaataaaat
      781 atttgaaaaa caggatggag aatcgtgtga aagattggcc caaattatgg aattatagga
      841 gacgttacga agaaatgtct gttgggatga aagcggcact ctggtatact gttgcaaatt
      901 ttattcaaaa gggttttgta tttctttcaa ctccgatttt aaccagaata cttaatgtta
      961 atgattacgg tacattgata ctttaccaat catggtttgt actatttgct atttttgcta
     1021 ccttgaatct ttctcaaaca gcttataata agggattagt agaatttgag aatgataaag
     1081 ataattttac attttctttg ttgtttttgt ctaagattat tacgataatt gttgcttttt
     1141 tgtattttac ttttaatata ctggttaaag ata
//
LOCUS       DAVKGU010000176         1148 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVKGU010000176 DAVKGU010000000
VERSION     DAVKGU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.178"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            149..1045
                     /locus_tag="VW077_002099"
     CDS             149..1045
                     /locus_tag="VW077_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9456037.1"
                     /translation="MDYKLISTYLDYCKTHKRLSSHTIRAYKNDLMQFYNSDYDHVES
                     YIEHLTQSNIKTNTLRRKIACMKVFYNYLKYQNIIEENPFNQLRFQFRTEKILPKTIP
                     YDILKNIFIYLEQKVVISKTDYQKQKAERNLLIISLLLSTGIRISELCHIHLKDINLS
                     NKTLHIIGKGKKERILFLGDQTTFNILETYINKTGKESNDFLFPGKHLPKPLSEQSVR
                     LILKRIVEQNSLSKTITPHMFRHSFATMLLDNDVDIRYIQQILGHSSISVTQIYTHVS
                     HSKQKEILSSFNPMSVIHSEIE"
BASE COUNT          453 a          181 c          146 g          368 t
ORIGIN      
        1 aatgtgacaa ttcaagacta aaaaatcatt atacaaatca aactactaaa aatgaattta
       61 tggtatacta gtgataacaa tttattatca ctatacaaag cacccaaata ggtttataat
      121 agcatgatta ttgataaagg agatttttat ggattataaa cttatttcta cttacttaga
      181 ttattgcaaa actcataagc gtttgagttc acacacgatt cgcgcttata agaatgatct
      241 tatgcaattt tataactcag actatgatca tgtcgaatcc tatatagaac atttgacaca
      301 atctaacata aaaacgaata cattaagaag aaaaattgct tgtatgaagg tgttttataa
      361 ctatctaaaa taccagaaca taattgaaga gaaccccttc aatcaattgc gttttcaatt
      421 tagaactgaa aaaatattgc ctaaaacgat tccgtatgac attctgaaaa acatttttat
      481 atatttagaa cagaaagtag ttatatctaa aactgactat caaaaacaaa aagctgaaag
      541 aaatctactt attatttccc ttttactttc aacgggtatc agaatttctg aactttgtca
      601 tattcatctc aaagatatta atctttccaa taagacacta catattatag gaaaggggaa
      661 gaaagagcgt atcctatttt taggagatca aacgaccttc aatatattag aaacatatat
      721 aaataaaacc ggaaaggaat ctaatgattt cctattccca gggaaacatt tgcctaaacc
      781 attgtcagag caaagtgtac gtttaatttt aaagagaatc gttgaacaaa atagcttatc
      841 taaaactatt acaccacata tgtttagaca tagctttgca acaatgcttc tagataatga
      901 tgtagatatt cgatacattc aacaaattct tggacacagt tctatatcag tcacacaaat
      961 ctatactcac gtatctcatt caaaacaaaa agaaatactt agttctttta atcctatgtc
     1021 agtgattcat tctgaaatcg agtaagagac atttctgaag gttacagttt actttatctt
     1081 aaatcaatgt ttgtagttcg actttagtta atagatttta tactcaacaa aagcaaaaaa
     1141 catataaa
//
LOCUS       DAVKGU010000177         1148 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVKGU010000177 DAVKGU010000000
VERSION     DAVKGU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.179"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..585)
                     /locus_tag="VW077_002100"
     CDS             complement(<1..585)
                     /locus_tag="VW077_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HEU9456038.1"
                     /translation="MTFPISEWKRNEEFDFFNKMDVPYLSMSSRIEVTQAINFHKKHS
                     ISLYAIISWCVMSAINSIPELLMDTDGKIVWQYNQRGCSFTTLTSEDKLNFSSFTMGD
                     NLIEFVSAFNINKQKAEEGQKPNIDKNNIAYLSCVPWIDFLHVSTPMNLSKIDTVPRI
                     TWGKVIQENQRYFCTVNLQINHGMGDGLHVSNFFV"
     gene            complement(582..>1148)
                     /locus_tag="VW077_002101"
     CDS             complement(582..>1148)
                     /locus_tag="VW077_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456039.1"
                     /translation="ESENSLDVDNISEFCGIAYGGIEIPLVAHLFKEKNEISFLFQNS
                     HYSTQEVEVKRFRDSRRNDSKSILLMDDNILTGRAMKNAAQKLSYRKYSVQFFHVRRL
                     GLNRLSQVIQENSMNELQRYLTGIYKGGIFPAPYSKIKFGTNIAKQYLDELQIFTLSG
                     DEILRLLYKNGLFSEESEVKVVRGNLYE"
BASE COUNT          367 a          217 c          151 g          413 t
ORIGIN      
        1 cacaaaaaaa ttagatacgt gtaatccatc tcccattcca tgattaattt gtaaattcac
       61 tgtacaaaaa tatctttggt tctcttgaat aacttttccc cacgttattc gaggaactgt
      121 atcaattttt gaaagattca taggagtgct tacatgtaaa aaatcaatcc atggaacaca
      181 agaaagataa gcaatgttat tcttgtcaat atttggtttt tgcccttctt ctgccttctg
      241 tttatttatg ttgaacgcag aaacaaattc aattaaatta tctcccatcg taaatgaaga
      301 aaaattaagc ttatcttccg atgtaagggt agtgaaagaa caacctcttt gattgtattg
      361 ccagacaatc tttccgtcgg tatccattag taattctgga atagaattta ttgcactcat
      421 aacacaccac gatattatgg cataaagtga tatcgaatgt tttttatgaa aattgatagc
      481 ctgcgttact tctattcgtg aactcataga taaataagga acatccattt tattgaaaaa
      541 gtcaaattct tcattccttt tccactctga tataggaaat gtcattcgta taaattcccc
      601 cttaccactt tcacttcact ctcttctgag aacaagccat tcttataaag tagtcttaag
      661 atttcatctc cagatagagt aaaaatttgt aattcatcta aatattgttt agcaatatta
      721 gtaccgaatt taatttttga atacggtgca ggaaaaatcc ctcctttata tattcctgtt
      781 aaatacctct gcaattcatt cattgaattt tcttgaataa cttgtgataa tctatttagg
      841 ccaagcctcc tgacatgaaa gaattggact gaatattttc ggtatgataa tttctgagca
      901 gcatttttca tagcccttcc tgtcaaaata ttatcatcca ttaataagat acttttcgaa
      961 tcatttctcc gactgtccct gaaccttttt acttcaactt cttgagtact gtagtggcta
     1021 ttttgaaata aaaatgatat ttcatttttt tctttaaata ggtgcgcaac caagggaatt
     1081 tctatcccgc cataagctat cccacaaaat tcactaatat tatctacatc caatgaattt
     1141 tcactttc
//
LOCUS       DAVKGU010000178         1088 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVKGU010000178 DAVKGU010000000
VERSION     DAVKGU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.180"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..409)
                     /locus_tag="VW077_002102"
     CDS             complement(<1..409)
                     /locus_tag="VW077_002102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456040.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLLKDRGIKNILIYIK"
BASE COUNT          457 a          168 c          128 g          335 t
ORIGIN      
        1 tttttatata aataagtata tttttaattc ccctatcctt caataatcgg tataaaagat
       61 actgactcat accttgatac caaataaaat tcggtatttc aataagagca tgaggtaata
      121 ttccttttat tgtagctact attccaatca tctgaccaga tatacccaat tgtattccta
      181 aagatagcaa atcaaaaagt tgcaataagg gcgaaatcgg aaaacagaat agatatagca
      241 aaaaatttgt agaatttgcc tgtaaaactg tcaatgtaaa ttgtaattcc agtttcggag
      301 gtatagtagc aaaagtctca ttttttaata taaagcttaa aagtaaacct agtaaaaaac
      361 agactataca aaataacaca tagtaaatca gaaaaccact ttttctcatc taatgccccc
      421 tactaaaatt acaaatatta tcagaaaaca atataaaacc agaaaactcc atctcaataa
      481 actaactgta cctttaatta aaaatagagc tttcttataa tagacatagg ctataaaata
      541 attgaatgga atcaataaca aattcaacaa aatacttttt atatcattac tataacttgt
      601 caccggaatc aaacataaca acaatgaaaa ttgtaagaaa gagattaaat gcgtcaacat
      661 cattagttgt gcattcttaa aaaagaaatt cattttagaa tgattaagag cagataattg
      721 aagtaaagct agatctaccc caattgagga tttatgattc cctattattg cctccattaa
      781 tgtaatatat attgaggtat aaagaaataa tattggtatt tttgtgtcat caattgataa
      841 catcgtcatc atataccaaa atagatagga aagaagaata ctaatcaaaa taaaagaaaa
      901 agttaaccct ttatttaacc aacaaaaacg aagcaatata aatagactgc tacgaaatct
      961 tttttgactc attcgactgc ttttaaaata gaaattactc gcatgattat ctaatatccc
     1021 cataactaaa ataaaataat tgagtagtat gacataaatt acaacttgtc cttgttctat
     1081 agacaata
//
LOCUS       DAVKGU010000179         1063 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVKGU010000179 DAVKGU010000000
VERSION     DAVKGU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.181"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>1063
                     /locus_tag="VW077_002103"
     CDS             <1..>1063
                     /locus_tag="VW077_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HEU9456041.1"
                     /translation="HLNLEKKYFKATLEAGRYTIIFDGLDEVNPSKRSWLDKEIKEFV
                     TLYNSNRYVLSSRPSEEFIGWNQFAEYEIKKLNREQALSLIDKLNYDEKVKRRFYREL
                     KDHLYDTHESFASIPLLLTIMLMTYEAGASIPNNLTDFYNQAFYTLYQRHDASKSGYK
                     RELKAKLTPEEFRNILAYIGLKTFFEGKVDFDRTTLDEIITKYCFKNNLELKTNDIVY
                     DATHSACMMLQEGVSLKFSHRSFQEYFAAVGVNQLDDKLQKQILIKWSEADRNNIVAH
                     KTFMNALFKIQKERTYKNLCIPIIERMDEKYSRMGDITEKIVTCFNYFICRKDSRENK
                     LELHFLLTKEVHFYFSLQF"
BASE COUNT          405 a          133 c          181 g          344 t
ORIGIN      
        1 atcatttaaa tttagaaaag aaatatttta aagcgacatt agaggctgga agatatacaa
       61 taatttttga tggtttagat gaggttaatc cttcaaaacg ttcttggttg gacaaagaaa
      121 ttaaagaatt tgtaacgtta tacaatagca atagatatgt tttgtcgtca agaccgtctg
      181 aggaatttat tggttggaat cagtttgctg agtatgagat aaaaaaatta aatagagagc
      241 aagcgttatc cttaattgat aagttgaatt atgatgagaa agtaaaaagg cgtttctaca
      301 gagaattaaa ggatcattta tatgatactc atgaatcttt tgcttctatc ccactcctat
      361 taacaattat gttaatgact tacgaagctg gtgcttctat tccaaataat ttaactgatt
      421 tttataatca ggccttttat acattgtacc aaagacatga tgcatctaaa tcaggataca
      481 aaagagaact aaaagcaaaa ttaactcctg aagaatttcg aaatatatta gcctacattg
      541 gtttaaaaac attctttgaa ggaaaagtag atttcgatag aactacactt gatgaaataa
      601 ttactaaata ttgttttaaa aataaccttg aattaaagac gaatgatata gtttatgatg
      661 cgactcatag tgcttgtatg atgcttcaag aaggcgttag tttaaaattc tctcaccgtt
      721 cattccaaga atactttgct gcagtaggtg ttaatcagct agatgataaa ctccaaaaac
      781 agatactaat taagtggtct gaagcagata ggaataatat tgtagcacat aaaactttta
      841 tgaatgcact ttttaaaata cagaaagaaa ggacttacaa aaatttatgt attccaatca
      901 tagaaagaat ggatgaaaag tatagcagaa tgggggatat cacagagaaa attgttacgt
      961 gtttcaatta ctttatctgt agaaaagata gtagagaaaa caaattagaa ttgcactttt
     1021 tactaacaaa ggaagttcac ttttatttta gcttgcaatt ttt
//
LOCUS       DAVKGU010000180         1057 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVKGU010000180 DAVKGU010000000
VERSION     DAVKGU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.182"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            40..702
                     /locus_tag="VW077_002104"
     CDS             40..702
                     /locus_tag="VW077_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9456042.1"
                     /translation="MNEQLPDEMTLAKQFACSRMTIKKALDLLVSEGLIFRKRGQGTF
                     VLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRIIHFKLEFANEFLAEKLQVALQS
                     PVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDILQKSIYNYIEGKLGLHIASATKI
                     LRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTPFEYSISRHRYDLFEFNSFALRH
                     SS"
BASE COUNT          347 a          169 c          192 g          349 t
ORIGIN      
        1 aatgagattc gtgaaaagat tctttgtaat aaatatacca tgaacgaaca attgcctgat
       61 gaaatgacat tagctaaaca gtttgcctgt agtcgaatga cgatcaaaaa agctttagac
      121 ttgttagttt ctgagggctt aatttttaga aaacgtgggc agggaacctt tgttctctct
      181 cgtggcagct caaaaagaaa attaatcgtt ccagaaagag atatccgggg actgacaaaa
      241 atatctgaag atgctcattc tacaattgac tcgaggatta ttcacttcaa attagaattt
      301 gcaaatgaat ttttagcaga aaaactacag gtcgctttgc agagtccagt ttataatatt
      361 taccgcctgc gtattattga cggtaaacct tatgttctgg aacaaactta tatgagtacc
      421 gatgttattc caggtattac tgaagatatt ttacaaaaat cgatttacaa ttacattgaa
      481 ggaaagttag gattgcatat tgccagtgct acaaaaatct tacgagcttc ttctagttca
      541 gaaaatgagc aacattactt gcagctcctt ccaacggaac cggtatttga agtagaacaa
      601 gtggcttatt tggataacgg aactccgttt gagtactcga ttagtcgtca tcgctatgat
      661 ttatttgaat ttaattcttt tgcattacga cattcctcct aggagaaaat gtgaaaatga
      721 agccaatctt ttacagactc tagtttaaga aaaatttaaa acagggcaag aaggtcccat
      781 ctatgcttaa atggtttctc ttttctaaat aagatggctt taaaagagtg atcgttgtat
      841 ccatcatgtt gaaaaatatc ttcgtatagc ttatagagta ggtactgaaa ttgttcacct
      901 gatctacttc ttatagttat ttagttttaa atagtgtttc aaacattctt acactgacga
      961 gaagtttttg agtcttttct tgtaacacat atagtatact gtggttagaa tagtagactg
     1021 tgacttctaa caaattgcta gaaatgaatt tcaatct
//
LOCUS       DAVKGU010000181         1056 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVKGU010000181 DAVKGU010000000
VERSION     DAVKGU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.183"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..559
                     /locus_tag="VW077_002105"
     CDS             <1..559
                     /locus_tag="VW077_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9456043.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(507..1052)
                     /locus_tag="VW077_002106"
     CDS             complement(507..1052)
                     /locus_tag="VW077_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9456044.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          379 a          167 c          155 g          355 t
ORIGIN      
        1 gaataaagac gaaacaacct cttatcctat atatgaaatc ccagaagcat ggagatatat
       61 taaatttgct agcctagtta acttcagaat aggaaaaact cctccacgca gtgaagctac
      121 tttttgggga actgaaatac cttgggtatc tatatcagat atgcctatat ctggttatgt
      181 aactaatacg agagaatcta tttctaaatt agcattaaaa tcaaagaaaa tagacatttc
      241 acctaaagga actttattaa tgagttttaa attatctatt ggaaaagttg ccatattgga
      301 tattcctgcc actcataatg aagccattat atcgatattc ccctatgcta ataaagaaaa
      361 tattatcaga gattacttaa tgatattttt gccacttatc tctactttag gtgattcaaa
      421 agatgctatc aaagggaaaa cattaaatag taccagtatc tccgaattat taatccctat
      481 ttctaatcat gaagagatga aaagaatcat ttccaaagtt gatttacttt ttcaaaaagt
      541 ttctcaactt tttgagtaat aagttcctgt tcctcaaaag gagctaacgg aattaatagc
      601 tcgcttagtg tagttttagg aatattatat aaagcttgac ctgatagttt agttattgct
      661 ttcaattgtt tataaaataa cggagaggac aagttaaata atagaaattt tgaaataatc
      721 tctgaacttt cgaatggtgt taattggaaa ataaatccac cagccacaac accatcatag
      781 tctttatcga ttcttgcaaa ctttccaata tgttctaaag aggttgatac aggtgttatt
      841 agctgattat gttttaaata aacttgctca gaggagatga attgtgtatc aatgtagtaa
      901 tcgttatcca acagagaaaa ttctaaaggc ttaatattac caccacgtat aattctaaca
      961 cctttattat taatgcttaa atcgcccttc ttgtaagaaa gacctgtatt cattgaaaaa
     1021 atatctttta tttttataac aacccaattc ataggt
//
LOCUS       DAVKGU010000182         1055 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVKGU010000182 DAVKGU010000000
VERSION     DAVKGU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.184"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..1049
                     /locus_tag="VW077_002107"
     CDS             <1..1049
                     /locus_tag="VW077_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9456045.1"
                     /translation="ENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCI
                     AASYIAGTKIIVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDR
                     VFLIDKGKLKKTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYK
                     FTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKK
                     ERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSL
                     SGGQKQRLAIASVMCKNSPFVFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHD
                     IEFLNEVADEIFEL"
BASE COUNT          416 a          114 c          202 g          323 t
ORIGIN      
        1 tggaaaatat cggtcttgca agagaagaga tggacaggcg tttgaaggat atgcttgaga
       61 tattcccgat aaaaaatctt ttgaacagaa atatatttaa tctatccggc ggtgaaaaac
      121 aaattctttg cattgcagct tcttatatag caggtacaaa gattatagtt atggatgagc
      181 cttcatcgaa tttagatatt aaaagcataa gtgttttggc aaagatgcta aagatattaa
      241 aagagaaagg cataagcata attgttgcag agcatagaat ttattatttg atggacatag
      301 ttgaccgtgt atttttaata gataaaggaa agcttaaaaa aacttatact agaagtgaat
      361 ttttaaagct agataaaaat gaattaaatg ctttaagttt aagagataaa gaattaagta
      421 aattaaaagt tccttattta aaagaaggtg gagagtatca gataaaaaat cttagctaca
      481 aatttactga tgatgagtgt ttaagcttaa aagatatttc gttcaagctt gggaaaattt
      541 atggcataat aggatccaac ggacgaggaa aatcaacgct tttaagatgt ttaataggtc
      601 ttgagaaaaa atcaaaagaa gaaatttatt ttaagggaga gaagctatct aaaaaagaaa
      661 gactcaaaaa ctcttcactt gttatgcaag atgtaaatca tcaattattc acagatgaag
      721 tattcaacga gcttagatta ggagtaaaga attttgatga agaaaaggcg aaaatcatct
      781 taaaagattt aggcctggac gaatttattg aaaggcatcc gatgagttta tcaggagggc
      841 aaaagcaaag acttgcaata gcatctgtta tgtgcaagaa ttctccattt gtcttttttg
      901 acgaaccttc aagtggtatg gattattcca atatgataaa aatatctgaa ctgattaata
      961 agtataaaac catggataaa ataattttta ttgtttccca tgatatagaa tttttaaatg
     1021 aagtggcaga tgaaattttt gaattgtaaa ataga
//
LOCUS       DAVKGU010000183         1027 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVKGU010000183 DAVKGU010000000
VERSION     DAVKGU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.185"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(1..1005)
                     /locus_tag="VW077_002108"
                     /pseudo
     CDS             complement(1..1005)
                     /locus_tag="VW077_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          365 a          177 c          133 g          352 t
ORIGIN      
        1 tagcaaatat tttttgcttc atataacgga aaatctctat tcacctcatt tggttcgata
       61 tcattcattt tatttgactc aattggatta attgaataca attttaaaaa aataggcttc
      121 gtaccaataa tagaggataa ttgacctcct aattcaccta gcgctgtaaa aataacacct
      181 gttagtgctc ctattgcttc aatagtacca attttcacta ttccttttat tgcaagatag
      241 cctgttaaaa aaacgagaga tatctgaaaa aaaaatattg agaaagaagc taatagcgcc
      301 tgctaacgtt tctacagttg tctttctttg tataaccatc tttaataaaa ttcctgcttc
      361 tttaattttc ttaggcaata catataaaag attcaaggat gctaacacat caaatccatt
      421 caatatagtc tcactagatt ttaaaaaagc ttcattttgg ttagttaaat ttagactaac
      481 ttctcgcatt ttcgatgcaa agatttttgg tacaagtagc ataatcatta atgaaaacaa
      541 ggtggctaca gtcaatgacc aatgatagtg attaagagtc acaactgcaa atatagtacc
      601 agaaattcct tttattacta aaaaaagttg tttaaaagcc tgatcattta aagtctgaac
      661 atcattattt agccacgaaa gatatgttcc tgatgattta ctatgaaatt cttgataggt
      721 agagttagag atgtctgtgg caactctatt tcgaatctct agattaaact cttggatcac
      781 ttcaacctga taatttttca ctacccagtc aaggaatatt atcccacacc agacaatcat
      841 ttggtagatt gacaatttca aaaaccgctc taaattcatc gcaattaatt cattcaacac
      901 cagagcatta atagttgctg cataaattag caataattga ccagcaacaa taaatatcgt
      961 taataaacta aattttttta tatttgattt tataatagta tacacaatag tttctcactt
     1021 tctaaat
//
LOCUS       DAVKGU010000184          986 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVKGU010000184 DAVKGU010000000
VERSION     DAVKGU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.186"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            186..416
                     /gene="blpU"
                     /locus_tag="VW077_002109"
     CDS             186..416
                     /gene="blpU"
                     /locus_tag="VW077_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9456046.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
BASE COUNT          358 a          100 c          214 g          314 t
ORIGIN      
        1 tagtaaaaaa gtgattagaa aacatctttt ttaaaaatag agatgatttt gaaacaaaaa
       61 agctaattca agacgtttcg atgccaattc aagatttgga tgaaaaaaat taatagatac
      121 tgttatacta aacttgtcaa gtttgtaaca agacaaatat taaaaataaa aaagaggtat
      181 tcgttatgaa tacaaaaacg atgtcacaat ttgaaattat ggatactgag atgcttgctt
      241 gcgttgaagg tggcggatgc aattggggag attttgccaa agcaggtgtt ggaggaggag
      301 cagcacgagg tcttcagcta ggaattaaaa caagaacatg gcaaggtgca gcaactggtg
      361 ctgtgggagg agctatactt ggaggtgtgg cctatgcagc gacatgttgg tggtaattat
      421 ggattttaaa agttttatta ttggtttagt agttggtata tttggtcctt atatggatga
      481 tttaattaga aaaaaatttt taaagtcttc ggagaagaaa acagaaaaat ctgttaaaaa
      541 ataatcaaaa ctataaatga tgaatctgaa tcaaaattat tttgcgcatg taaataggag
      601 tcttatagta acgagtcaaa aaaggagtaa ctatgaatcg taatttagaa cggtgttatc
      661 tattctgact aggaatagat cataccagag gtggcttaga aatagcgggg acattagaaa
      721 ttgaaggaac tttaaatagg atgtcgtaaa tgttgctatc aatgatttat ttgtcccaag
      781 cttgcctagg gtgtcagtaa aaaatcattt tcctttcata tcatattttt agtaggtagg
      841 atgtttgttc tgtctatttt ttgccaaaaa aagtgcagtc gaagtgttta tttggagttt
      901 tatatatatt ttcgataaaa taggtgtaga aattaggaaa gttgaagaaa agaaatgaag
      961 aaggttttaa aatatttttt agtatt
//
LOCUS       DAVKGU010000185          981 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVKGU010000185 DAVKGU010000000
VERSION     DAVKGU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.187"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            121..843
                     /locus_tag="VW077_002110"
     CDS             121..843
                     /locus_tag="VW077_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEU9456047.1"
                     /translation="MIPKKIHYCWFGGKPLPNDVKRCIASWRKFCPDYEIIEWTEKNF
                     CIQNQNQFVKDAYKNKAWAFVSDYARLKIVYENGGIYLDTDVEIIKKLDLLLDNTAFF
                     GVHQVNHLVNTGLGFGAEKGSFILKELLDSYDEVKFDINKKDELLCPELNTQIFKQLG
                     YVYSNSVIKSQYFTIYPEEYFDPISLGDEVANILSDKTFSIHHYNASWTSLMNRVKRK
                     IIRKLGRSNVSELKRILKGKTR"
BASE COUNT          343 a          120 c          176 g          342 t
ORIGIN      
        1 agaactagat aatcttattc attttaccaa tcctataggt aatggaaatg taggtgcaga
       61 cctattttat tataataaat actttgatgt tgttaattgg ttgaatggag agtactaagt
      121 atgataccta aaaaaattca ttattgttgg tttggtggca aaccattacc aaatgatgtg
      181 aaacgatgta tagctagttg gagaaagttt tgtcccgact atgaaattat tgaatggact
      241 gaaaaaaatt tttgtattca aaatcaaaat caatttgtaa aagatgcgta taaaaataag
      301 gcatgggctt ttgttagcga ctatgctaga ctcaaaattg tttatgaaaa tggaggtatc
      361 tatttagata ctgatgttga gattattaaa aagctagatt tgttactgga taacacagca
      421 ttctttggtg ttcatcaagt taatcatcta gttaatacag gattaggttt tggggctgaa
      481 aaggggtcat tcattttaaa agaattatta gattcttatg atgaagtgaa atttgatatt
      541 aacaagaaag acgagctatt atgtccagaa ttaaacacgc aaatatttaa acaactaggt
      601 tatgtttatt ctaattcagt tataaaaagt caatatttca caatctatcc cgaagaatac
      661 ttcgatccca tttcattggg agatgaagtt gctaatattt tatcagataa aacattttct
      721 attcatcatt ataatgcatc ttggacttcc ttgatgaatc gggtgaagag aaaaataatt
      781 cgtaaactag gtagaagtaa tgtctcggag ttaaaaagaa tattaaaggg gaaaactaga
      841 tgagaatatc accatcgaag ctgatatttg cattttttag cagtttttat attcttgtaa
      901 tccttttccc gagaggaatt ttcctagggc attctacttt agcttctcgt ttatggatta
      961 tttacaaaga aagtttatat c
//
LOCUS       DAVKGU010000186          946 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVKGU010000186 DAVKGU010000000
VERSION     DAVKGU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.188"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>946
                     /locus_tag="VW077_002111"
     CDS             <1..>946
                     /locus_tag="VW077_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEU9456048.1"
                     /translation="CGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMM
                     VAETPLVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDF
                     SRLPKIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQV
                     KIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYK
                     AIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLF
                     HFQNKDPEKFFGLIEDNLKQVHPIFQTVFKTFLKNKEKI"
BASE COUNT          300 a          198 c          155 g          293 t
ORIGIN      
        1 tgtggaagtc taatgaagaa atatgacttt caaaaaccgt ctaagatccc ttacctcgaa
       61 acaactggta tgccttctag aattctcctt agaaaacgcc gtttcaagtg ctatcactgt
      121 tcaaaaatga tggttgctga gactcccctg gtaaagaaaa atcaccaaat ccctcgtatc
      181 atcaaccaaa aaattgctca aaagttaatt gaaaaaattt ctatgactga tattgcccat
      241 cagctggcca tttcaacttc aactgtcatt cgcaagctca atgactttca ctttgagcat
      301 gatttttctc ggcttccaaa gattatgtct tgggatgagt atgccttcac taagggaaag
      361 atgagtttca ttgcgcaaga ttttgataat cttaatatta tcactgttct tgaaggcaga
      421 acacaagctg tcatccgaaa tcactttctt cgctacgata gagccgttcg ttgtcaagtg
      481 aaaatcatta cgatggatat gtttagtcct tactatgact tggctaaaca gctttttccg
      541 tgtgctaaaa tcgttctaga tcgtttccat attatccaac atctcagccg tgccatgagt
      601 cgttttcgtg ttcaaattat gaatcagttt gaacgaaaat ctcatgaata caaggctatc
      661 aagcgttact ggaaactcat ccaacaggat agtcgtaaat tgagcgataa acgtttttat
      721 cgccctactt ttcgcatgca cttaacaaat aaagaaattc ttgacaagat tttaagctat
      781 tcagaagact tgaaacacca ctatcagatc tatcaactct tactttttca ctttcagaac
      841 aaagaccctg agaaattttt cggactcatt gaggacaatc tgaagcaggt tcatcctatt
      901 tttcagactg tctttaaaac ctttctaaag aacaaagaga aaatcg
//
LOCUS       DAVKGU010000187          934 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVKGU010000187 DAVKGU010000000
VERSION     DAVKGU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.189"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(123..839)
                     /locus_tag="VW077_002112"
     CDS             complement(123..839)
                     /locus_tag="VW077_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9456049.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT          276 a          149 c          185 g          324 t
ORIGIN      
        1 ggttctattt ctataaaata ttccacaaat tatagaatct tccaaaaata ggtaagcgct
       61 acctttttgg tgtggtataa taagcataga aaaagcccaa gcgattagct caggctttct
      121 tcttagtgat cacggtcaca tgagataaat ttaatcttgt agtaatcaga tcgtttgtaa
      181 gtttcactgt attctaaaac ttggccagtt gattcgagtt tggtgatttt agtttgtagg
      241 acagtaggga attgttcatc gactccgagg actgaagccg catgttctgg agttggaaag
      301 actatttcgt tgatttcttc aaagtgttca tcattcatgt gaatgtggta gtctaacttg
      361 aaacgattat agatagaact atagtattca aggtttggat aatttgcgtt gatatattgt
      421 tctgggatgt aggatgtatg gtagatataa acgacaccgt ttgattcgcg gatacgttca
      481 atcttgtagt agaattgatc gccgcgtaga cccaattttt ccaagtaaac aagcttgttt
      541 ccgcgttcaa ttgaaagaac agttacctta tcatctttag cattgaagag ttcaatatct
      601 gaaaactcta caagcttgtg tttgcgcgca cgtgaaacga aggttccttt tccttgttgg
      661 cggacaatat agccatcttt ggcaaggtcg tttaaggcgc gaacaactgt gatagagctg
      721 acatcgtaca ttgaaatgag ttctgcttca gtgtaaaatt tatctccact gctaaactgc
      781 ccagagatga ttttattttt taattcgtct tttatgtatt gatatttggg aattgccata
      841 tttttacctc tattttcctt ctccatagaa aattttacca taaaagcgaa aaaaatagta
      901 gtcttttgaa taaaaaatta gaataatagt ttga
//
LOCUS       DAVKGU010000188          927 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVKGU010000188 DAVKGU010000000
VERSION     DAVKGU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.190"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            1..878
                     /locus_tag="VW077_002113"
                     /pseudo
     CDS             1..878
                     /locus_tag="VW077_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          348 a          147 c          184 g          248 t
ORIGIN      
        1 aaatgggacg taagccaaaa aagagacctg aagagaggac agaattagaa cgtcttcaag
       61 cagaaaatga gtacctgaga gcggagaatg ccatcctaaa aaagttaaga gaactccgat
      121 tgaaggagga aaaagagaaa gaagaaagac agaaattgtt caagaattaa tgactgagtt
      181 ttcgttagat cttcttctaa aagccattaa actagctcgt tggacctact actatcactt
      241 gaaacagcta gacaaaacag ataaggacca agagcttaaa gctgaaattc aatccatttt
      301 tatcgaacac aagggaaatt atggttatcg tcgggttcat ttagaactaa gaaatcgtgg
      361 ttatctggta aatcataaaa gagttcaagg cttgatgaaa gtactcaatt tacaagctaa
      421 aatgcgacag aaacgaaaat attcttctca taaaggagac gttggtaaga aggcagagaa
      481 tctcattcaa ggccaatttg aaggctctaa aacaatggaa aagtgctaca cagatgtgac
      541 tgaatttgcc attccagcaa gtactcaaaa gctttactta tcaccagttt tagatggctt
      601 taacagcgaa attattgctt ttaatctttc ttgttcgtct aatttagaac aagtacaaac
      661 aatgttggaa caggcattca cagagaagca ctatggtgga gtccttcttt gggattctga
      721 aatcggaaat gttttatggc tatgagaaaa catttaaatc acttaatcaa ttggaacaag
      781 ctattataga ctatattgat tattacaaca acaagagaat taaggtaaaa ctaaaaggac
      841 ttagccctgt gcaatacaga actaaatcct tcggataaat taattgtcta actgtttggg
      901 gtgcagtaca gaactggcgt tttattt
//
LOCUS       DAVKGU010000189          922 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVKGU010000189 DAVKGU010000000
VERSION     DAVKGU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.191"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            351..536
                     /locus_tag="VW077_002114"
                     /pseudo
     CDS             351..536
                     /locus_tag="VW077_002114"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          354 a          171 c          127 g          270 t
ORIGIN      
        1 cttgtttaat atatgtttta catatagtat ttagcgccac atagtactga actctctcca
       61 aaaacggtta tgcctctttg aatagggcgt tatcacaaga aaagcatctc cacgtttcaa
      121 cttcatatgg ctcaaaaaca atcaattgat gctaaaacct gtacctagat gtttcggttc
      181 ataaaaccat gaaactgtaa aagtggatga aattgatagc gatagtcaaa tcaagaggca
      241 tcataactct aaaaagtcac aatatataag ttcatcctcg gaaaaatatc attctaattg
      301 ttgaaatgcc tacatgaaaa gaaacgtcaa atactcatga aacaacgaat acaggtatca
      361 aaactatgac aaaacaagtc cctaaattta ctaaagacac tgctcaactt tacacctgta
      421 aatggttgtt gtataataaa gttacaaaga tgtacgacca cactgttgta aatcatagtg
      481 ttcgcgaata tattactgat agcatttcta caaatacaat tgaaaaggct ggataggctt
      541 taaacgaaat atgtcagtgt tttggcattc ctagccttca tatcatttaa agaattgtat
      601 agacaaaatt tttcccaata cagacactcg taacaactgc ttcatttttc taccaacata
      661 tttaggaaca ggataagata caagaggatt aatccataga tcagttctat accaatctaa
      721 gacaaataag ctaaaaaaac gattgataat aagcaaatag attccaaatt ttctctatct
      781 gctcatttta ataaacaata ctagtgtaac tatccttcca gtcagaagct tgtcaaatca
      841 caccgaaaat tcttctaaaa tttatctcgt taggcaatca agcaaaaact cgacgatagt
      901 acaaacatta tcatacagaa tt
//
LOCUS       DAVKGU010000190          887 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVKGU010000190 DAVKGU010000000
VERSION     DAVKGU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.192"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>887
                     /locus_tag="VW077_002115"
     CDS             <1..>887
                     /locus_tag="VW077_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9456050.1"
                     /translation="FKALEGYRKVFLPTTSNNEWFKAQTKAYIVEEKSSIPEVSKKQS
                     EQGTKYSIGIYDRLTSPSWKYQSMVLPLLTLPEEKTVFMIANISTIGFGAYDRYRSKV
                     HPKGDNLNKFVEDNVREAAKRFRDHYDYWYKILEPENREKLYRSLLVYDAFKFGRDNT
                     EDKVTYQADFETDHPAIKYFFGPAGNNVVHNGHGAYATGDAFYYMAYRMLDKDGAVTY
                     THEMTHNSDREIYLGGYGRRSGLGPEFYAKGLLQAPDHPYDPTITINSVLKYDDSENS
                     TRLQVADPTQRFNSAEDLH"
BASE COUNT          345 a          123 c          163 g          256 t
ORIGIN      
        1 atttaaagca ctagaaggat atagaaaagt attcttacca acaacttcta acaatgagtg
       61 gtttaaagct caaactaaag catatattgt tgaagaaaaa tcttctattc cagaagtaag
      121 taaaaaacaa tcagaacaag gaactaagta ttctatagga atttatgata gattaactag
      181 tccgtcatgg aaatatcaaa gtatggtctt accactatta actttacctg aagaaaaaac
      241 agtatttatg atagcgaata tttcaacaat aggatttggt gcttatgata gatatagaag
      301 taaagtccat cctaaaggag ataacttaaa taaatttgta gaagacaatg taagagaagc
      361 tgcaaaaaga tttagagatc attatgatta ttggtataaa atattagagc ctgagaatag
      421 agagaaactt tatagaagtt tattagtata cgatgcattt aaatttggtc gagataatac
      481 tgaagataaa gtaacttatc aagctgattt tgaaactgat catccagcta taaaatactt
      541 ctttggacct gcggggaata atgttgttca taatggacac ggagcatatg ctaccggaga
      601 tgcattctat tatatggcat atcgtatgtt ggataaagat ggagctgtaa catatactca
      661 tgaaatgaca cataactctg atagagaaat ttatcttgga ggatatggaa gaagaagcgg
      721 attagggcct gagttttatg ctaagggctt attacaagca cctgaccacc catatgatcc
      781 aacaataaca attaactctg tgttgaaata tgatgattca gaaaattcaa caagacttca
      841 agtagctgat ccaactcaac gatttaatag tgcagaagac ctacata
//
LOCUS       DAVKGU010000191          881 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVKGU010000191 DAVKGU010000000
VERSION     DAVKGU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.193"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(8..81)
                     /locus_tag="VW077_002116"
     tRNA            complement(8..81)
                     /locus_tag="VW077_002116"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Ile,seq:cat)
     gene            complement(95..168)
                     /locus_tag="VW077_002117"
     tRNA            complement(95..168)
                     /locus_tag="VW077_002117"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Met,seq:cat)
     gene            complement(174..247)
                     /locus_tag="VW077_002118"
     tRNA            complement(174..247)
                     /locus_tag="VW077_002118"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VW077_002119"
     tRNA            complement(263..336)
                     /locus_tag="VW077_002119"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(346..431)
                     /locus_tag="VW077_002120"
     tRNA            complement(346..431)
                     /locus_tag="VW077_002120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Leu,seq:taa)
     gene            complement(439..510)
                     /locus_tag="VW077_002121"
     tRNA            complement(439..510)
                     /locus_tag="VW077_002121"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Gly,seq:gcc)
     gene            complement(533..605)
                     /locus_tag="VW077_002122"
     tRNA            complement(533..605)
                     /locus_tag="VW077_002122"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Thr,seq:tgt)
     gene            complement(624..705)
                     /locus_tag="VW077_002123"
     tRNA            complement(624..705)
                     /locus_tag="VW077_002123"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(669..671),aa:Leu,seq:tag)
     gene            complement(711..783)
                     /locus_tag="VW077_002124"
     tRNA            complement(711..783)
                     /locus_tag="VW077_002124"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Lys,seq:ttt)
     gene            complement(808..880)
                     /locus_tag="VW077_002125"
     tRNA            complement(808..880)
                     /locus_tag="VW077_002125"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Asp,seq:gtc)
BASE COUNT          232 a          257 c          199 g          193 t
ORIGIN      
        1 attcaaatgg accttgtagg acttgaacct acgaccactc ggttatgagc cgagagctct
       61 aaccagctga gctaaaggtc cgacaagatc attatagcgg cgaaggggat cgaacccccg
      121 acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgccat gaatcgggaa
      181 gacaggattc gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta
      241 cttcccgagt taaatagaaa aatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctcca tattatgccg aggaccggaa
      361 tcgaaccggt acgatcgtta ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc
      421 gccaccccgg cctctctaag cgaacgacgg gattcgaacc cgcgaccccc accttggcaa
      481 ggtggtgttc taccactgaa ctacgttcgc actgttttct tctatctaaa aatgccggct
      541 acatgacttg aacacgcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      601 ccggctcatt tgttatatct taatgcgggt taagggactt gaacccccac gccgttaagc
      661 gccagatcct aaatctggtg cgtctgccaa ttccgccaaa cccgcatata tgacccgtac
      721 tgggctcgaa ccagtgaccc attgattaaa agtcaattgc tctaccaact gagctaacga
      781 gtctaaaata acttgcgtta ccttaaacgg tcccgacggg aatcgaaccc gcgatcttcg
      841 ccgtgacagg gcgacgtgat aaccgctaca ctacgggacc t
//
LOCUS       DAVKGU010000192          870 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVKGU010000192 DAVKGU010000000
VERSION     DAVKGU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.194"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..475)
                     /locus_tag="VW077_002126"
     CDS             complement(<1..475)
                     /locus_tag="VW077_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9456051.1"
                     /translation="MNLSYELSIWEILLRCFLAILFGGIVGYERGVKNQAAGIRTYLL
                     VCLGTCVVMMTNQFITSRYGSGDPSRMGAQVISGLGFLGAGTIMVTNQKVKGLTTAAG
                     LWSSATIGLAIGIGFYEGAILAGVVLIAVLTLFTPLKDYLNSKTKIADYYLVFSSL"
     gene            complement(477..>870)
                     /locus_tag="VW077_002127"
     CDS             complement(477..>870)
                     /locus_tag="VW077_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU9456052.1"
                     /translation="IAYTIQKLPFSYRMIRAVFFSIDNDMEEAARSMGASSFYTMVRV
                     IIPYILPVVLSVVVLNFNSLLSDYDLSVFLYHPLFQPLGIVIKQSTDETATLNAQAMM
                     FVYSVILMIMSSAALYLSSLFQGKRGKR"
BASE COUNT          314 a          173 c          134 g          249 t
ORIGIN      
        1 ctaaagaact aaaaaccaag tagtaatcag caattttagt tttcgagttt aaataatctt
       61 taagaggagt aaatagagtt aaaactgcta tgagtacaac tccggctaaa atagcacctt
      121 cataaaaacc aatgcctata gctaaaccaa tagttgctga agaccataat ccagctgctg
      181 ttgttaaacc tttaactttt tgattagtta ccatgattgt tcctgcccct aggaaaccca
      241 agccactaat tacttgggct cccattcgac taggatcacc gctaccataa cgactagtga
      301 tgaactgatt tgtcatcatt acaacacaag ttcccaaaca aactagtaag taggttctaa
      361 tccctgctgc ttggtttttg actcctcgct catagccaac aatgccaccg aaaagaatcg
      421 ctaaaaagca cctaagaagt atttcccaaa tactcagttc gtatgaaaga ttcatattat
      481 ctcttacctc gtttaccttg gaataggctt gataaataaa gagctgcact agacataatc
      541 attaaaatta cagaataaac aaacatcatt gcctgtgcat ttaaagttgc tgtttcatca
      601 gtagactgtt taataacgat tcccaatggt tggaaaagtg gatggtacaa gaatacagat
      661 aagtcatagt cagataataa agaattaaag tttaacacaa ctactgataa tacgactggt
      721 aaaatatacg gtataataac tcgcaccata gtgtagaaac tagatgcacc catactacgt
      781 gcagcttctt ccatatcatt atctatactg aaaaatacag cccgaatcat tctataagaa
      841 aatggtaatt tttgaatagt atatgcaata
//
LOCUS       DAVKGU010000193          849 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVKGU010000193 DAVKGU010000000
VERSION     DAVKGU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.195"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>849
                     /locus_tag="VW077_002128"
     CDS             <1..>849
                     /locus_tag="VW077_002128"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9456053.1"
                     /translation="FLQLYEKKFLYTKKIKQLYCTFDNQFLPDRFVEGKCPNCGTHSR
                     GDQCDNCSAILDPIDLVDKRCSICSNEPEVRETEHFYYVFSEFQNLLETYLNDAEETV
                     RWRKNAINLTKRYLREGLPDRAVTRDLPNGIPVPIDGFRDKKIYVWFEAVAGYYTASV
                     DWAQKLQNNITDFWNNRTKSYYVHGKDNIPFHTIIWPAILSGLEIEPLPEYIISSEYL
                     TLENKKISTSNNWAIWLNDIIKKYDADSIRYFLTINAPEMKDANFSWREFIYSHNSEL
                     LGSYGN"
BASE COUNT          305 a          119 c          152 g          273 t
ORIGIN      
        1 atttttgcaa ttatacgaga agaaattttt gtatacaaaa aaaattaaac aactctattg
       61 tacatttgat aatcaattct tacctgatag gttcgttgaa ggaaaatgcc cgaattgtgg
      121 aacccattca agaggagatc aatgtgataa ttgctcagca attttagatc ctatagattt
      181 agttgataaa agatgttcta tttgttccaa tgaaccagaa gttcgtgaga cagaacactt
      241 ttattatgtt tttagtgagt ttcaaaattt gttagagact tacttaaatg atgctgaaga
      301 aacagtgaga tggcgaaaaa atgcaataaa tctaactaaa aggtatttac gggaaggttt
      361 acctgatcga gctgttactc gtgatctacc aaatgggatt ccagttccca ttgatggttt
      421 tagagataaa aaaatatatg tctggtttga agcggtagca ggttactaca cagcaagtgt
      481 tgattgggcg caaaaacttc aaaataatat tactgatttt tggaacaatc ggacaaagtc
      541 atattatgtt catggaaaag ataacatccc atttcatact attatttggc cggcaatact
      601 ctcaggttta gaaatagagc ctcttccaga gtatattatt tctagtgaat atttaacgtt
      661 agaaaacaaa aaaatatcaa caagtaataa ttgggctata tggcttaacg atatcataaa
      721 aaaatatgat gctgattcaa tccgatactt cctaacaata aatgctcctg agatgaaaga
      781 tgcaaatttt tcttggagag aatttattta tagtcataac tcagaattat taggatcgta
      841 cggtaattt
//
LOCUS       DAVKGU010000194          778 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVKGU010000194 DAVKGU010000000
VERSION     DAVKGU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.196"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            1..778
                     /locus_tag="VW077_002129"
                     /pseudo
     CDS             1..778
                     /locus_tag="VW077_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          278 a           69 c          167 g          264 t
ORIGIN      
        1 atgccatgga tgggttgaat ataacttaaa aaagaaattc ttgacttatt ttgatttttg
       61 gacatattct atgggagatg cttttatttg tgtatctgaa actatgaaaa aaagattgga
      121 gagtataata aaaaaataag aagatagttg caataaataa cggaattaat gtatcaaata
      181 gcgatttaga tgtagtaggt gttcaagact ttaaaaaaga attttgtatt ccaaataaca
      241 aaaaaatcat ttgttatgtt ggaaggttgg atccagaaaa aaggccggat agattccttg
      301 aatttgcaga aaaattattt ttagtcagag aagatgtgat ttttattatg gctggaaatg
      361 gtagtatgtg ggctgctttg aaagaaaaaa tttgccattt gaaatgtaga gataatttca
      421 ggttacttgg tgaaatctat ccagctacta tagtttacca aatatcggat ttattgtata
      481 ttccttctga tacagaagga atacctatgt gtgttttaga gtcaatgtca caaggaactc
      541 cggttttagc tagtaatgtt ggcgggttaa gtgaaattat tgaacatagg gttgatggat
      601 ttttatttga gaaggaagat gttgagggag tgtgtgcttg tgctaatttt ttactcaatg
      661 attctgagta tttgaaatat ataggtgaga atagtaaatc aaaaataaga aaacattttt
      721 ctgtgcaaaa aatgtttgta gaaaccatga gagtatatga tgaattatta gagaagag
//
LOCUS       DAVKGU010000195          763 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVKGU010000195 DAVKGU010000000
VERSION     DAVKGU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.197"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>763
                     /locus_tag="VW077_002130"
     CDS             <1..>763
                     /locus_tag="VW077_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9456054.1"
                     /translation="QEIDVPYDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGI
                     NWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAI
                     HDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPP
                     LAEQQRIIEAIESALEKVDEYAESYNRLEQLDKKFPDKLKKSILQYAMQGKLVEQDPN
                     DESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQ"
BASE COUNT          290 a          104 c          134 g          235 t
ORIGIN      
        1 caagaaattg atgttcctta tgatattcct gatacttggg agtgggtgag gttttctaca
       61 ttggttgaaa ttgtcagagg tggctctcca cgaccaatca aagattatct tacttctgaa
      121 gtagatggaa taaattggat aaaaataggt gatactgaaa agggtgaaaa gtatataaat
      181 aatgttaaag aaaaaatcaa aaaatcaggg cttaacaaaa ctagatttgt aaaaaaaggt
      241 acatttttgt taactaattc tatgagtttt ggtagacctt atattttgaa tgttgatggt
      301 gcaatacacg atggatggtt ggctatttcg aactatgaaa actcattaaa taaagattac
      361 ctattctata ttctttcatc aaatgtagtt tattctcaat ttctatctct aattagtgga
      421 gctgttgtga aaaacttgaa tagtgataaa gttgcttcta ttcttatccc tctccctccg
      481 ctagctgaac aacaacgcat aatagaagca atcgaatcag ctttagaaaa agtagatgaa
      541 tatgctgaaa gttataatag actagaacag ctagataaaa aatttccaga taaactaaaa
      601 aaatctattc ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa
      661 tcagtcgaag ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc
      721 aagattaaaa agaaagattt ggacatttct attgtttccc aag
//
LOCUS       DAVKGU010000196          759 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVKGU010000196 DAVKGU010000000
VERSION     DAVKGU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.198"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            281..499
                     /locus_tag="VW077_002131"
     CDS             281..499
                     /locus_tag="VW077_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9456055.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
BASE COUNT          330 a           83 c          129 g          217 t
ORIGIN      
        1 aatttaaaga ttctttaaag agtcttattt tgtgatgaaa atttaatatg taaatctcag
       61 acgatagaaa ttaaaaactc tatcgtcttt tttatactca aaattaggag gtaaaaatgg
      121 taaggataag aggtcccact taaaacaatt tatggcaaaa taaggacgga ataacacaac
      181 aaattctcta aaacaaatca ctaaatcaat gtaagattga atgaaatcaa tatttatgct
      241 ataattaaat aaatctaatg aagaaaaaaa gagggatatt atggcactta actataaacc
      301 attatggata cagttagcaa aaaaaggact aaagaaaaca gatgtaatag ctatggcagg
      361 acttacaaca aatgttatgg cacaaatggg aaaggataaa ccaattacat ttaagaattt
      421 agaaagaata tgtaaggctt tatcttgcac tcctaatgat attattagtt ttgaagataa
      481 ttttagtgac gaggaataga aaatgacttt aaggacagaa gatcaagtta gggattatgc
      541 aagagaagta taggctttaa tgaagttgaa gaaaacatca atcaaggtac tggtcaaata
      601 actactttta atcaattagg cttcaaggga tattcagata agccagatgg ttggtattta
      661 cctaaaaata tgaatgatgt agcaataatc cttgaaacaa aatcagaaga aagagatatt
      721 agcaaacaaa tttttattga tgagttaatg aaaaatata
//
LOCUS       DAVKGU010000197          747 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVKGU010000197 DAVKGU010000000
VERSION     DAVKGU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.199"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
BASE COUNT          248 a          138 c           92 g          269 t
ORIGIN      
        1 atcatttacc tacttccaag atgctgaaaa atactaagtc acactattat gatgttatta
       61 tttccatttt tattttaaca gatgagtcaa ttctttcaac taccccctcc cttataacta
      121 tattcatgaa gtattcttga taataaacct catttaactt ctcaaagtag tcagcttctg
      181 caactgctat tatctcataa cagattgatt tggtttcaat aaatgaaagc tagaagcctt
      241 gtcagctacc ctgcacagtt ggtgtttttt catttttctc ttcaaaaaaa ttggtcggaa
      301 gttataataa aaaggtgatg tgtttgtggt gagttgaatt aagagacaaa ctcactttgt
      361 cttaactttt aaattgccta acaaaaatac ggagattatt ctctaagctt taactgcaac
      421 attatgtctc tacaaccaac gataagtcca gaaatctacc caaataacca aacaacctac
      481 atttgttgaa ctttgatgac aagtaggaaa ttttacattt gataaacact attttgtacg
      541 cattttgcac ataatttaac gtgctttcca tcaatttagt taaggactta catagaaaac
      601 aatccgtttt tgcttatgtt aaattagtag aattagacaa aaacaaaacc gccgttcgca
      661 ctgttatata ttcattttta gtacttacta ttctaaaaaa gatatctatc tttcaattcc
      721 tcaatttctt gactacttat tcctagt
//
LOCUS       DAVKGU010000198          735 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVKGU010000198 DAVKGU010000000
VERSION     DAVKGU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.200"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            173..379
                     /locus_tag="VW077_002132"
     CDS             173..379
                     /locus_tag="VW077_002132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9456056.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KKTLTPTKESIDFLRNKIMKQVNN"
     gene            313..>735
                     /locus_tag="VW077_002133"
     CDS             313..>735
                     /locus_tag="VW077_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEU9456057.1"
                     /translation="MDPYQGVNRLPAKQNYEASKQLTDARFKRLVGVQRTTFEEMLAV
                     LKTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIATDFGIHESNLIRRSQWV
                     EVTLVQSGVTISRTPLSSEDTVMIDATEVKINRPKKTIS"
BASE COUNT          283 a          132 c          134 g          186 t
ORIGIN      
        1 gtcaaggaaa attaacccac tttaatttct tttttaaccc gggttaaaaa agaaattaaa
       61 gtgggttaaa aaagaaatta actgagtaaa aactctcgaa attcaattag ctatattatc
      121 actcaacttg aacaaatacc agaaccacaa aaaacagatg taaaggaaaa atatggactt
      181 aaaatttgaa ggggttgatt tagaatacaa aaaagctaaa aacaatcttc cagagtcttt
      241 ctgggaaacc tactcagcat ttgcaaatac aaatggcggg aaaataattc tcgggataga
      301 tgaaaaaaaa acattgaccc ctaccaagga gtcaatagac ttcctgcgaa acaaaattat
      361 gaagcaagta aacaactaac cgatgcacga tttaagcgtc ttgttggtgt tcagcgcacc
      421 acttttgaag agatgttagc tgtgttaaaa acagcttatc aacttaaaca cgcaaaaggt
      481 ggacgaaaac ctaaattaag cctagaagac cttcttatgg ccactcttca atatgtgcga
      541 gaataccgca cttatgaaga aattgcgact gattttggta ttcacgaaag caacttaatc
      601 cgtcggagcc aatgggttga agtaactctt gttcaaagtg gtgttacgat ttcaagaact
      661 cctctcagtt ctgaggacac ggtaatgatt gatgcgacgg aagtaaaaat caatcgccct
      721 aaaaaaacaa ttagc
//
LOCUS       DAVKGU010000199          714 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVKGU010000199 DAVKGU010000000
VERSION     DAVKGU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.201"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            157..441
                     /locus_tag="VW077_002134"
     CDS             157..441
                     /locus_tag="VW077_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456058.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            434..583
                     /locus_tag="VW077_002135"
     CDS             434..583
                     /locus_tag="VW077_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456059.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFMAL"
BASE COUNT          274 a          111 c          111 g          218 t
ORIGIN      
        1 attcttttct aagatgaaaa acagaatttt tcgattgtga tttaaagcaa caagaagatt
       61 ttcagtatca tcctatagat acgagctaat taagaaaaac tacattttcg aatataaact
      121 acaataatat aaactaaatt ttataggagg aagacaatgg attggtacga ttatatgata
      181 caggcatcca aacaatcaca attcaacgca agccattggt ttcgctattt gcgaaaagtt
      241 atttttgaag actattctta tttaacaaac caagatgtag aaaagttgct agactccaaa
      301 gaactaaccc gttttcaaaa aattagcttg aagtatgcct ttcaagagca tactccaact
      361 cataaatatg tgatttcatt aaataaacct gctaagttaa ccaatgttca aaaattgatg
      421 gagaaataca aacatggata aaatgaaacc ggtcttccaa gccctaaata aggaattaat
      481 tcaggaaaat ctgactttaa caattatctg tgtcgatggt tatgtcttag aatatcatgg
      541 tttacgtgcc acacaagatg ttgatgcttt tatggctcta taatatttgt agtgggtaaa
      601 tcccctatgg atattatgga gcctattttt gtgtagaaaa aaagtcccat atgacctata
      661 atgaaaagcg acaaaacaac tcattagaaa gaatcatatg gaacaattac attt
//
LOCUS       DAVKGU010000200          696 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVKGU010000200 DAVKGU010000000
VERSION     DAVKGU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.202"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..647)
                     /locus_tag="VW077_002136"
     CDS             complement(<1..647)
                     /locus_tag="VW077_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9456060.1"
                     /translation="MGIKNSRGKYISFLDDDDEYMSDRILKLMACFKKSRMKNLALVY
                     SYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQ
                     DGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADEEYYNYCRKYFNL
                     LSFNERILVTKKYYSLNIKRLLLIGDKCKALKVIKKAREEKIFNEFLFLKYMLLY"
BASE COUNT          227 a          133 c           69 g          267 t
ORIGIN      
        1 tgatataata acatatattt caaaaaaaga aattcgttaa aaattttttc ttctcttgcc
       61 ttcttgatta cttttaaagc cttgcatttg tctcctatta atagtaaccg ctttatgttt
      121 aaagaataat atttctttgt aaccaatatt ctctcgttga aactcaataa attaaaatat
      181 ttcctacagt aattataata ttcttcatct gcattaattg ttttttgtgt cactccagtg
      241 ataccgtttt ctttactgtg agcgtagtaa ttcaccaaga attctcgcac tatatcaatt
      301 tggtatcctt gagcaagtag ttttaataaa acaacaccgt cctgatgtga atctattttc
      361 tcaaaaccat taattaattc tagcacctct tttttacaca accaaaatga cgtacctgct
      421 atattgtgaa ccatttgaac aaacaaggga tttccaacaa aatcggtctt ctcctcttct
      481 cgtgtaccat ttggataaat tattattcca taactataaa ctaaagctaa attcttcatt
      541 ctactctttt taaaacaagc catcaacttt aaaattcgat ctgacatata ttcatcatca
      601 tcgtctaaaa atgatatata cttacctcta gaatttttga tacctatgtt tctggcatta
      661 gttgcaccta aatcttcatt acttaaaatt aactta
//
LOCUS       DAVKGU010000201          688 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVKGU010000201 DAVKGU010000000
VERSION     DAVKGU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.203"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>688
                     /locus_tag="VW077_002137"
     CDS             <1..>688
                     /locus_tag="VW077_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU9456061.1"
                     /translation="PTDYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPFGFSN
                     KLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTA
                     NIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVCLDPRI
                     GNFYNNLSFGFGGYCLPKDTKQLKKEFIEINAPVIEAIDISNTNRKQFIVKQILERKP
                     KIVGIYKLGMK"
BASE COUNT          251 a           84 c          127 g          226 t
ORIGIN      
        1 ccgacagatt atgatgagaa aattggtagt tttaatacat atgaaatcga acaaacggta
       61 tcgaagattc tgagggtaaa acctaatgga aagattattt taaagtcaac agttccgttc
      121 ggcttttcaa acaaattaaa aaggctgttt gatacaaaaa atatcatttt tgtccctgaa
      181 tttttgagag aaggttgttc tatatatgat aatttatatc caagtcgcat agttgttgga
      241 gatgagacag ttgaaggaag aaaaattgca gagttgttcc tttcgattag tactcatagt
      301 actgccaata ttaaaaatgt tatgttagtt tctcctactg aagcagaagc aattaagctt
      361 ttttctaaca cattcttagc tctccgtgtt gcttttttta atgaactaga ttcttttgct
      421 gagaggagaa gtttaaatgc tgaagttgta ataaaaggtg tttgtttaga tccaagaatt
      481 ggaaattttt ataataatct ttcttttgga tttggaggat attgtcttcc caaagatact
      541 aaacaattaa aaaaagaatt tatagaaata aatgccccag tgatagaagc gattgatatt
      601 tcaaacacaa atagaaaaca gttcatagtt aaacaaatat tggaacgaaa gccaaagata
      661 gtaggaatat ataaattggg gatgaaat
//
LOCUS       DAVKGU010000202          687 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVKGU010000202 DAVKGU010000000
VERSION     DAVKGU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.204"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            99..395
                     /locus_tag="VW077_002138"
     CDS             99..395
                     /locus_tag="VW077_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEU9456062.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
BASE COUNT          233 a           90 c          114 g          250 t
ORIGIN      
        1 ataaaatata gacgaacatt tgaagagagt taggaataca acatgtgcga attaactctc
       61 aataaaacat acataatata caaaaggagt acactaaaat gaaatctaaa agaatggaat
      121 ttcataataa atttcttatt gtttcagcaa tgttagcagt cattagttgg ttaagcttag
      181 gtgttgtttc ttttcctatg ttggctggga cattaggaat ttcgacaaaa gctgctgcta
      241 ctgtagtcaa tttgattagt gcttatagta cagttacagc agtaatttca attgtaggtg
      301 caatcacagg agtaggttct attggttcgg gtattgcagc aacagttctt tatatattga
      361 aaaagaaagg tgctgctaaa gcagctctat ggtagaacta gatgaaaata ctttctaaaa
      421 aagaatgtga aagtatttta ggttatctta attttattgg gataaccctt ttgttttttg
      481 ttatcataag tttgtcaatc tattttaaaa gctatcttcc ttttcaaggg aaattatttt
      541 tacttttttt ggtagtagat tacgctactt tgaatcgtta taatatgaaa ctgcctatcc
      601 aaagtaaaaa aataaatact ttctatataa ggaaaaacta tatttttctc tatcttcttg
      661 ccattgtcaa gaaatattat tctttcc
//
LOCUS       DAVKGU010000203          687 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVKGU010000203 DAVKGU010000000
VERSION     DAVKGU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.205"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..202)
                     /locus_tag="VW077_002139"
     CDS             complement(<1..202)
                     /locus_tag="VW077_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEU9456063.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGG"
     gene            complement(271..540)
                     /gene="blpJ"
                     /locus_tag="VW077_002140"
     CDS             complement(271..540)
                     /gene="blpJ"
                     /locus_tag="VW077_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEU9456064.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
BASE COUNT          205 a          181 c           82 g          219 t
ORIGIN      
        1 cacctccaag tatagctcca cccgcagcac cagttgctgc accttgccat gttcctgttt
       61 taatgcctag ttgaagacct cttgctgctg ctcctccaac acctgctttg gcaaaatctc
      121 cccaattgca tccgccacct tcaacgcaag caagcatttc agtatccata acagaaaatt
      181 gtgacatcat ttttgtatcc atgacaaata ctcctttttt aaaaaactaa aataaatcag
      241 aatagaatcc tcataatttt actataagtc ttaccaactt agtcccaatt tatcaccaac
      301 catacctcct aagcatgtta atccaccccc aattgcacca atgtgtgctc caacaaatgc
      361 accagcaagt ccagctactc ctaaagtggc caaacctgct ccagttccac cagttataat
      421 tcccgtagtg actcctgtaa tcagtgcatt ttgacaatca gtggagctat accccccttc
      481 aactttcgca agcatttcag tatccataac ctctaactgt gacaacattt ttgtattcat
      541 gatgaatacc tcctttttat tttcaatttg ttaccaaagt cttaaattca ataaacaaat
      601 agatttttta tagtatcttt ttgattttct taaaaaagta tacacgtcta ctatcttctt
      661 aaaggtagca gtacctattt tttagtc
//
LOCUS       DAVKGU010000204          668 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVKGU010000204 DAVKGU010000000
VERSION     DAVKGU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.206"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            83..445
                     /locus_tag="VW077_002141"
     CDS             83..445
                     /locus_tag="VW077_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9456065.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT          223 a           83 c          121 g          241 t
ORIGIN      
        1 atctacaacc tcaaaacagt gttttgagca acctgaggct agtttcctag tttgctcttt
       61 ggttttcatt gagtataaca gattgttaga agttggttta aatttcctaa tcagtttgtt
      121 cacatttacc ttcgatatat tatatcccat agttaaggtt ggtcatacag atgattatag
      181 tcatggagcc gtaaaactta gtgtttcttt agttgacaaa gatgccatga aaaaaatatc
      241 tgtaactgta ataggatatt ttgaaataaa tatagatgaa aatatcaccg atattctata
      301 cgtaaatggt actgctattc tttatcatta tttacgttct attgtttcaa tagtttcggc
      361 aattgatagc agtgaagcaa tgttgctacc taccattaat gttttagagt tactagataa
      421 atctcaacct tttgaagaag aataatttat tagctcacta aattgagggt aaggaaaagt
      481 aaaatcagta agaaaaatgt cttgcattat acagcaacct tttgggaatg agtagatgga
      541 ttgaataaaa ttttattaag agtggatgat ttatctgtag attattattg gacagttagt
      601 cttgaagtag tctaagaatt aggttataat cagtagaatc cttgctaata atgaggaggt
      661 tagtttat
//
LOCUS       DAVKGU010000205          659 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVKGU010000205 DAVKGU010000000
VERSION     DAVKGU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.207"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(95..277)
                     /locus_tag="VW077_002142"
     CDS             complement(95..277)
                     /locus_tag="VW077_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9456066.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIASELVAGLR"
     gene            336..659
                     /locus_tag="VW077_002143"
                     /pseudo
     CDS             336..659
                     /locus_tag="VW077_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          239 a          107 c          107 g          206 t
ORIGIN      
        1 gaaatttttt tattttgtgt gattcgaatg tgataatggc aacagtctca ctaaagaaat
       61 gaaaatcata catagaaatt tttaaaaatt ttctctatcg taaacctgcc actagttcag
      121 aagctatact agctgattcc aaacgtaata tggtcatctc atctaccttc cctaataatg
      181 gcattgcacc ataccaaaca atagtcttat tcaccaaaag aataggcgtt actatttttg
      241 attgagtaaa agacacttta attttctggc tcttcaactg tgaactacac cccaaaagtt
      301 agacagaaaa aatctaactt ttggggtgtt ttattatgaa actaacttat gatgataaag
      361 ttcagatcta tgaacttaga aaacaaggat atagcttaga gaagctttca aataaatttg
      421 ggataaacaa ttctaatctt aggtacatga ttaaattgat tgatcgttac ggaatagagt
      481 tcgtcaaaaa aggaaaaaat cgttactatt ctcctgattt aaaacaagaa atgattcata
      541 aagtctgaca tgaaggctgg actaaagata gagtttctct tgaatacggt ctcccaagtc
      601 gtacgatact tcttaactgg ctagcacaat acaggaaaaa cgggtatact attgttgag
//
LOCUS       DAVKGU010000206          640 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVKGU010000206 DAVKGU010000000
VERSION     DAVKGU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.208"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            106..447
                     /locus_tag="VW077_002144"
     CDS             106..447
                     /locus_tag="VW077_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU9456067.1"
                     /translation="MYGEGKISIGKNFHSGLNCQIITSFHNYDLGDKIPYDESYIHKD
                     VLIEDNVWVGNNVIILGGAIIEEGAIIQAGSTVAGRIPAGAIAGGHPAKPFRFRNMEH
                     YNRLKRQKKFH"
     gene            457..>640
                     /locus_tag="VW077_002145"
     CDS             457..>640
                     /locus_tag="VW077_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein"
                     /protein_id="HEU9456068.1"
                     /translation="MNIVSYLKKNGITHTLKIINEYKMQELFDSLVSIFTKQKELRDS
                     IVIECHTDFENNGGAFY"
BASE COUNT          228 a           76 c          141 g          195 t
ORIGIN      
        1 aaaagaaagg ccgcacaatg cagtgggaga gtgtatacgg gaggcaaaac gtttgttact
       61 gcaaatacgt accttgatga aaatgtttca tttaatggta tgtcaatgta tggtgaaggg
      121 aaaataagca ttggtaaaaa ttttcattca ggattaaatt gccaaattat cacttccttt
      181 cataattatg acttgggtga taaaattcct tatgatgaga gttacataca taaagacgtc
      241 ttaattgaag ataacgtttg ggtagggaat aatgtgataa ttttaggtgg tgcgattatt
      301 gaggaaggtg caatcattca agcaggaagt acagttgctg gaagaattcc agcaggtgcg
      361 attgcaggtg gacatccggc aaaaccattc agatttagaa atatggaaca ttataatcgg
      421 cttaaacggc aaaaaaaatt tcattagagg ttaagaatga atattgtaag ttatctaaaa
      481 aaaaacggta ttacacatac tttaaaaatt attaatgaat ataaaatgca agagctgttt
      541 gatagtcttg ttagtatttt tacaaagcag aaggaacttc gtgacagtat tgtcattgaa
      601 tgtcatactg attttgagaa taatggtggt gcgttctatg
//
LOCUS       DAVKGU010000207          639 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVKGU010000207 DAVKGU010000000
VERSION     DAVKGU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.209"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>639
                     /locus_tag="VW077_002146"
     CDS             <1..>639
                     /locus_tag="VW077_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU9456069.1"
                     /translation="DLFIKIVLDAIIRIIFGLYCFYLLFFSGRRQLKIEKKYIKYGLS
                     YSLPLMPHFLSHYILNQSDRLMINMFDGKEKLAIYSLAYSVSMIMFLFTNAINQSIMP
                     YTFQALKKKDYRGIHGSTKWLFITVGGVTVLSILFAPELIVILGGNKYKESIWLVPPI
                     AISVYFLFVYSMFSNISFYYKMNKLISLVSTGAALSNIILNYIFINIFGYQAA"
BASE COUNT          193 a           82 c          104 g          260 t
ORIGIN      
        1 gatttattca ttaaaattgt attagatgct attatccgaa ttatctttgg tttatattgt
       61 ttttatctct tgtttttttc agggagaagg caacttaaga tagaaaaaaa atatattaaa
      121 tatggtttga gttattcatt acctttgatg ccgcatttct tatcgcacta tattttgaat
      181 cagtctgatc gtttgatgat taacatgttt gatggtaagg agaaattagc tatatatagt
      241 ctagcttatt ctgtatcaat gattatgttt ttgtttacta atgcaattaa tcaatcaata
      301 atgccgtata catttcaagc tttgaaaaaa aaggattatc gtgggataca tgggagcacc
      361 aaatggcttt ttataacagt tgggggagtc acagttctct caattctatt tgcaccagaa
      421 ctaattgtta ttttaggcgg caataaatat aaggaatcaa tttggctagt acctccaatt
      481 gctatctcgg tttatttcct ttttgtgtat tctatgttta gtaatatcag tttttattat
      541 aagatgaata aactgatttc gttagtttcc acaggtgcag ctctttcaaa tattatttta
      601 aattatattt ttattaatat atttgggtat caagctgca
//
LOCUS       DAVKGU010000208          633 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVKGU010000208 DAVKGU010000000
VERSION     DAVKGU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.210"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..578)
                     /locus_tag="VW077_002147"
     CDS             complement(<1..578)
                     /locus_tag="VW077_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9456070.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIREEVVQNSTYSMADYVSIYTSYIVSANGEKFSPYQTLTANSL
                     CTFDFDNIRDDFLFLLHENSYYLCVSIGFKRELLEKHLSSININPESFYGALLQTNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKAN"
BASE COUNT          212 a          111 c           81 g          229 t
ORIGIN      
        1 tcatttgcct ttgctttaaa gaaaaaatca gcggcaggag cgtccatctt acaatttaat
       61 atttccattg ccactctttc taaggccttt gtaagtataa tttgattagt ttgaagtaaa
      121 gccccataaa atgattctgg attaatgtta atagatgata gatgtttttc caaaagttct
      181 cttttaaatc caatagaaac acaaagataa tatgaattct cgtgtaataa aaataggaag
      241 tcatctctta tattgtcaaa gtcaaaagta cataatgagt ttgcagtaag tgtttgataa
      301 ggactaaact tttctccatt tgcgctgact atgtagcttg tgtatattga aacataatct
      361 gccatactat aggtactgtt ttgaactact tcttctctga tataaaaatc atgtatatcg
      421 ataatgaagc tgcctccttc ataaaaccaa tataggcctt ctgcataagt tgaattctct
      481 tttttccaac aaaatgtatg acctgcactt gaatacttct cattgttttt acattcatct
      541 actttcatat aatcttttac aaaatcataa tatgacataa cacactatcc cttttagaca
      601 atattccaat tagccttatt aattcaaaac tat
//
LOCUS       DAVKGU010000209          623 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVKGU010000209 DAVKGU010000000
VERSION     DAVKGU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.211"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..>623
                     /locus_tag="VW077_002148"
     CDS             <1..>623
                     /locus_tag="VW077_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU9456071.1"
                     /translation="SQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDI
                     DSTHFTTYGKQEGVAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFI
                     TPVLERFNQLLFRMDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDE
                     DLTILPHSAYSETLYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTN"
BASE COUNT          169 a          155 c          112 g          187 t
ORIGIN      
        1 tcacagccaa ccttatcccg ttttctttcc agaactgacg aggaaacagt ccatagtttg
       61 cgatgcctca accttgaatt ggtcgaattc tttttacagt ttcaccagct aaaccaactc
      121 attgtagata tcgattctac ccatttcaca acttatggca agcaagaagg tgttgcttat
      181 aacgcccact atcgtgctca tggctatcat cctctttatg ctttcgaggg gaagacaggt
      241 tattgtttca atgcccagct tcgtcctggt aatcgttatt gttctgaaga ggcagacagc
      301 tttatcacac ctgttttaga acggtttaat caacttctct ttcgaatgga tagtggcttt
      361 gcgaccccaa aattatacga tttaattgaa aaaacagggc aatactacct cataaaactc
      421 aagaaaaata ctgttctgag ccgtcttgga gacctttccc tcccttgccc acaggatgag
      481 gacttaacca tcttgcccca ctccgcctac tcagaaactc tctatcaagc aggatcttgg
      541 tcgcacaagc gtcgtgtctg ccagttctct gaacgaaaag aaggaaactt gttctacgat
      601 gttatttctc tcgttacaaa tat
//
LOCUS       DAVKGU010000210          602 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVKGU010000210 DAVKGU010000000
VERSION     DAVKGU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.212"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            276..>602
                     /locus_tag="VW077_002149"
     CDS             276..>602
                     /locus_tag="VW077_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU9456072.1"
                     /translation="MNPLKECYEKNGILIAAHRGTNGGNILQNTSKAYLNSIRHNADM
                     FEVDVIRSKDGEFFAFHDGQEPLVFGEKIDIRTLSSEEIQRKECFNSLNEPILQKIEK
                     ISDVLKN"
BASE COUNT          263 a           71 c           83 g          185 t
ORIGIN      
        1 tgaaattgaa ggttagattt aaagtaataa agaataatat tacgctctaa aattagataa
       61 atttcaaata attcaaataa ttaaggatac ctatcactat tataaagtaa ctgaacaaca
      121 ttttatattt aacaattatt taaaataata aaattataac attataacat tatataaatt
      181 attttaaaaa ataatgaaac gttccaactt atctattgac acgttccaag aaatcggata
      241 caatgtaatt aacaataaag ataggagaaa ataatttgaa tccattaaaa gaatgctatg
      301 aaaaaaacgg tatactaatt gcagcacacc gtggtacaaa cggaggaaat atattacaaa
      361 acacatcaaa agcctatttg aattccatac gccataacgc agatatgttt gaggttgatg
      421 taattcgttc aaaagatgga gaattttttg catttcacga tgggcaagaa cctttagtat
      481 ttggtgaaaa aattgatatt agaactttga gttctgaaga gatccaaaga aaggaatgtt
      541 ttaattcact gaatgaaccc atattacaaa agattgaaaa aatttctgat gtattaaaaa
      601 at
//
LOCUS       DAVKGU010000211          567 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVKGU010000211 DAVKGU010000000
VERSION     DAVKGU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.213"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..>567)
                     /locus_tag="VW077_002150"
     CDS             complement(<1..>567)
                     /locus_tag="VW077_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9456073.1"
                     /translation="LLLKAIKLARSTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNY
                     GYRRIYLELRNRGYLVNHKRVQGLMKVLNLQAKMRQKRKYSSHKGDVGKKAENLIQGQ
                     FEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLE
                     QAFKEKHYENTILHSDQGWQYQHDSYHR"
BASE COUNT          150 a           98 c          110 g          209 t
ORIGIN      
        1 aaccgatgat aagaatcgtg ttggtattgc cagccttggt cactatggag aatcgtattc
       61 tcatagtgct tctctttgaa tgcctgttcc aacattgttt gtacttgttc taagttgggt
      121 gaagttgaaa gattataggc gataatttca ctgttaaagc catctaaaac tggtgataag
      181 taaagctttt gagtactggc tggaatggca aattctgtca catctgtgta gcacttttcc
      241 attgttttag agccttcaaa ttggccttga atgagattct ctgctttctt accaacgtct
      301 cctttatgag aagaatattt tcgtttctgt cgcattttag cttgtaaatt gagtactttc
      361 atcaagcctt gaactctttt atgatttacc agataaccac gatttcttag ttctaaataa
      421 atccgacgat aaccataatt tcccttgtgt tcgataaaaa tggattgaat ttcagcttta
      481 agctcttggt ccttatctgt tttgtctagc tgtttcaagt gatagtagta ggtcgaacga
      541 gctagtttaa tggcttttag aagaaga
//
LOCUS       DAVKGU010000212          557 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVKGU010000212 DAVKGU010000000
VERSION     DAVKGU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.214"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            <1..429
                     /locus_tag="VW077_002151"
     CDS             <1..429
                     /locus_tag="VW077_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456074.1"
                     /translation="DHVRKNKADQDSKPDEDKEHDEVSEPTHPESDEKENHAGLNPSA
                     DNLYKPSTDTEETEEEAEDTTDEAEIPQVENSVINAKIADAEALLEKVTDPSIRQNAM
                     ETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
BASE COUNT          213 a           88 c          113 g          143 t
ORIGIN      
        1 gaccatgttc gtaaaaataa ggcagaccaa gacagtaaac ctgatgaaga taaggaacat
       61 gatgaagtaa gtgagccaac tcaccctgaa tctgatgaaa aagagaatca cgctggttta
      121 aatccttcag cagataatct ttataaacca agcactgata cggaagagac agaggaagaa
      181 gctgaagata ccacagatga ggctgaaatt cctcaagtag agaattctgt tattaacgct
      241 aagatagcag atgcggaggc cttgctagaa aaagtaacag atcctagtat tagacaaaat
      301 gctatggaga cattgactgg tctaaaaagt agtcttcttc tcggaacgaa agataataac
      361 actatttcag cagaagtaga tagtctcttg gctttgttaa aaaaaagtca accggctccg
      421 atacagtagt aaaatgaatg gagcatattt tatggagaag taacctttcg tgttacttct
      481 cttttttaga aaaacgtaac agaaacttga caaaagtaat tttaaatagt aaactattta
      541 ctggttaatt aaatggt
//
LOCUS       DAVKGU010000213          500 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVKGU010000213 DAVKGU010000000
VERSION     DAVKGU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.215"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(90..>500)
                     /locus_tag="VW077_002152"
     CDS             complement(90..>500)
                     /locus_tag="VW077_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9456075.1"
                     /translation="WLQYNDSWYYLNASGAMATGWLQYNGSWYYLNANGSMATGWLQY
                     NGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQW
                     FKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV"
BASE COUNT          135 a          113 c           96 g          156 t
ORIGIN      
        1 aatacgaaca taaatttatt caatcgaacc ttatctttgt ttcaaaatta aaatgtcaaa
       61 tgttcttaac atgatttaat ttaatcggct taaacccatt caccattggc attgacttta
      121 tagccatcta cagttgtgtt gactgcaagg gcacctaaac cattgacata gtaccattta
      181 tctgatactt tgaaccattg gcttgctttc atagcacctg atgcttcaag atagtaccag
      241 gtatctccat ctttcaccca acctgttgcc attgaaccgt tagcgttgag gtagtaccat
      301 gaaccgttga ctttagccca acctgttgcc atagcgccgt tagcgttgag gtagtaccat
      361 gaaccgttgt attggagcca acctgttgcc attgaaccgt tagcgttgag gtagtaccat
      421 gaaccgttgt attggagcca acctgttgcc atagcgccgc tagcgtttag gtaataccat
      481 gaatcgttgt attggagcca
//
LOCUS       DAVKGU010000214          494 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVKGU010000214 DAVKGU010000000
VERSION     DAVKGU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.216"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            62..494
                     /locus_tag="VW077_002153"
                     /pseudo
     CDS             62..494
                     /locus_tag="VW077_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          163 a           91 c          104 g          136 t
ORIGIN      
        1 acaactaact gatgcacgat ttaagcgtct tgttggtgtt cagcgtacca cttttgaaga
       61 gatgttagct ggattaaaaa cagcttatca acttaaacac gcaaaaggtg gacgaaaacc
      121 taaattaagc ctagaagacc ttcttatggc cactcttcaa tatgtgcgag aataccgcac
      181 ttatgaacaa attgcggctg attttggtat tcacgaaagc aacttaatcc gtcggagcca
      241 atgggttgaa gtaactcttg ttcaaagtgg tgttacgatt tcaagaactc ctctcagttc
      301 tgaggacacg gtaatgattg atgcgacgga agtacaaatc aatcgcccta aaaaaacaat
      361 tagcgaatga ttctggtaaa aagaaatgct acgctatgaa ggctcaagcg attgtcacaa
      421 gtcaaggaag aattatttct ttggatatca ctgtgaacta ttgtcatgat atgaagttgt
      481 tcaaaatgag tcgc
//
LOCUS       DAVKGU010000215          477 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVKGU010000215 DAVKGU010000000
VERSION     DAVKGU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.217"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            complement(<1..443)
                     /locus_tag="VW077_002154"
     CDS             complement(<1..443)
                     /locus_tag="VW077_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001857240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9456076.1"
                     /translation="MKNISLEIDKGIYRVEGENGSGKSTLLQLLAGLETFDSGLKNCV
                     SKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINTFFDNKIGDNYMTAS
                     TGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISMK"
BASE COUNT          161 a           88 c           58 g          170 t
ORIGIN      
        1 tctttcatgg aaattaagcg tttagcaatc atatcaatac ttcttgcatc cactgttgat
       61 aaagtctcat caataaaaat ataatcccaa tttttagcaa aaattaagga taagcctaat
      121 ttgaattttg ttccagtaga tgctgtcata tagttatctc cgattttatt atcaaaaaaa
      181 gtattaattt gttctttatc ctcaaatgat agatggattt gaaaagaatc acacaacaac
      241 tgcaaatttt cttctatagt aaatggaaca accccaagct gattggtatc aaggtatagt
      301 acatctttgg agacacaatt ctttaaacca ctatcaaaag tctctagccc agctaaaagt
      361 tgtagaaggg tgctctttcc tgaaccattc tctccttcaa ctctgtaaat tcccttatca
      421 atctctagtg atatattttt caatatacat ttatcataac ttttagattc attttca
//
LOCUS       DAVKGU010000216          408 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVKGU010000216 DAVKGU010000000
VERSION     DAVKGU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.denovo.218"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
BASE COUNT          160 a           68 c           51 g          129 t
ORIGIN      
        1 aagattttga cataattgtt gcataataaa gcaataggag ctttagctta tattttctag
       61 tattcgtctt aaagaacacc aaatagataa acactattcc taatctgagc tatctaatct
      121 attaaaaatc aatagaactt catacaacaa gtggtaaagt agtaaatccg ttccagatca
      181 aaaaactcat tctgctcttg caagaacact tgatgttcca acaacatatt ttgaatcagt
      241 atacaagatt atgatcccct atcttcagct ctcttcaaaa aattaagatt ctgatgctga
      301 aaaaagaaga atccttgatt ttatgagtct agtaaatagt gaatacccaa ttgatgaaag
      361 ttttaattat caaaactata tttcaaatgc agaaattcca gcaaaata
//
LOCUS       DAVKGU010000217         9646 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.guided.077, whole genome shotgun sequence.
ACCESSION   DAVKGU010000217 DAVKGU010000000
VERSION     DAVKGU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.guided.077"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            44..160
                     /locus_tag="VW077_002155"
     CDS             44..160
                     /locus_tag="VW077_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEU9456077.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            176..2095
                     /gene="tet(M)"
                     /locus_tag="VW077_002156"
     CDS             176..2095
                     /gene="tet(M)"
                     /locus_tag="VW077_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEU9456078.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2214..2381
                     /locus_tag="VW077_002157"
     CDS             2214..2381
                     /locus_tag="VW077_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEU9456079.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2441..2794)
                     /locus_tag="VW077_002158"
     CDS             complement(2441..2794)
                     /locus_tag="VW077_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9456080.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3299..3721
                     /locus_tag="VW077_002159"
     CDS             3299..3721
                     /locus_tag="VW077_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9456081.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            3718..3948
                     /locus_tag="VW077_002160"
     CDS             3718..3948
                     /locus_tag="VW077_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9456082.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            4409..4612
                     /locus_tag="VW077_002161"
     CDS             4409..4612
                     /locus_tag="VW077_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEU9456083.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            4694..5910
                     /locus_tag="VW077_002162"
                     /pseudo
     CDS             4694..5910
                     /locus_tag="VW077_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(6125..6712)
                     /locus_tag="VW077_002163"
     CDS             complement(6125..6712)
                     /locus_tag="VW077_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9456084.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKLLCISELTILQILYCASLIS
                     FLWMYGQRKQVVKVNMKTRMKWLGIGFVSLLIINLCFSLIHAQGTTNQANLIGLQHQV
                     PWFSFLLLLINASMVEEFLYREILWNLVRKLDIRVALTSILFVLAHHPGTILAWCLYV
                     SLGMFLGLVRYKSDLWGSMGLHLVWNLSVYVLFFL"
     gene            complement(6715..6948)
                     /locus_tag="VW077_002164"
     CDS             complement(6715..6948)
                     /locus_tag="VW077_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9456085.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            complement(6961..7341)
                     /locus_tag="VW077_002165"
     CDS             complement(6961..7341)
                     /locus_tag="VW077_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9456086.1"
                     /translation="MIDLYLSKNCHRNQLLLDFFQKYGIEVTPHSISEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLKLTDFIEMILKNPELTIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            complement(7334..7783)
                     /locus_tag="VW077_002166"
     CDS             complement(7334..7783)
                     /locus_tag="VW077_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9456087.1"
                     /translation="MKALNKESILDCDELETELHDAEIKQLDEQLFLIPNYPCEFEVT
                     FLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDHQNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKKPINFSNLLDSLIVSEYQMEPNLWEVSHD"
     gene            complement(7767..9125)
                     /gene="dcm"
                     /locus_tag="VW077_002167"
     CDS             complement(7767..9125)
                     /gene="dcm"
                     /locus_tag="VW077_002167"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU9456088.1"
                     /translation="MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQT
                     EGEIEFHDIRDVSDDEFKKLRGKVDIICGGFPCQAFSIAGRRLGFEDIRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
                     RVFIIGHSRKKGIRLLFPFRREGQATNSETLKTLGNLNPSKSGMSGKVYYSEGLAPTL
                     VRGKGEGFKIAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFK
                     ETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLE
                     RPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGILIDGQFYRLRIRR
                     ITPKECFRLQGFPDWAFEAARRVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDES
                     IK"
     gene            complement(9224..9472)
                     /locus_tag="VW077_002168"
     CDS             complement(9224..9472)
                     /locus_tag="VW077_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IFN-response binding factor 1"
                     /protein_id="HEU9456089.1"
                     /translation="MKRKVEKVTKYDQDYIWGLVQDQFRREGFSETASEIAMTDFERI
                     YQYALDNVRFVRRAEVLAEFVFNGLYSVWNNRVRKGGG"
BASE COUNT         3188 a         1754 c         1754 g         2950 t
ORIGIN      
        1 agggcttagt tttttgtacc cagtttaaga atacctttat catgtgattc taaagtatcc
       61 ggagaatatc tgtatgcttt gtatgcctat ggttatgcat aaaaatccca gtgataagag
      121 tatttatcac tgggattttt atgccctttt gggtttttga atggaggaaa atcacatgaa
      181 aattattaat attggagttt tagctcatgt tgatgcagga aaaactacct taacagaaag
      241 cttattatat aacagtggag cgattacaga attaggaagc gtggacaaag gtacaacgag
      301 gacggataat acgcttttag aacgtcagag aggaattaca attcagacag gaataacctc
      361 ttttcagtgg gaaaatacga aggtgaacat catagacacg ccaggacata tggatttctt
      421 agcagaagta tatcgttcat tatcagtttt agatggggca attctactga tttctgcaaa
      481 agatggcgta caagcacaaa ctcgtatatt atttcatgca cttaggaaaa tggggattcc
      541 cacaatcttt tttatcaata agattgacca aaatggaatt gatttatcaa cggtttatca
      601 ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa cagaaggtag aactgtatcc
      661 taatatgtgt gtgacgaact ttaccgaatc tgaacaatgg gatacggtaa tagagggaaa
      721 cgatgacctt ttagagaaat atatgtccgg taaatcatta gaagcattgg aactcgaaca
      781 agaggaaagc ataagatttc agaattgttc tctgttccct ctttatcatg gaagtgcaaa
      841 aagtaatata gggattgata accttataga agtgattacg aataaatttt attcatcaac
      901 acatcgaggt cagtctgaac tttgcggaaa agttttcaaa attgagtatt cggaaaaaag
      961 acagcgtctt gcatatatac gtctttatag tggcgtactg catttgcgag attcggttag
     1021 aatatcggaa aaggaaaaaa taaaaattac agaaatgtat acttcaataa atggtgaatt
     1081 atgtaaaatc gataaggctt attccgggga aattgttatt ttgcagaatg agtttttgaa
     1141 gttaaatagt gttcttggag atacaaagct attgccacag agagagagaa ttgaaaatcc
     1201 cctccctctg ctgcaaacga ctgttgaacc gagcaaacct caacaaaggg aaatgttact
     1261 tgatgcactt ttagaaatct ccgacagtga cccgcttctg cgatattatg tggattctgc
     1321 gacacatgaa atcatacttt ctttcttagg gaaagtacaa atggaagtga cttgtgctct
     1381 gctgcaagaa aagtatcatg tggagataga aataaaagag cctacagtca tttatatgga
     1441 aagaccgtta aaaaaagcag agtataccat tcacatcgaa gttccaccga atcctttctg
     1501 ggcttccatt ggtctatctg tagcacagct tccattaggg agcggagtac agtatgagag
     1561 ctcggtttct cttggatact taaatcaatc gtttcaaaat gcagttatgg aggggatacg
     1621 ctatggctgt gaacaaggat tgtatggttg gaatgtgacg gactgtaaaa tctgttttaa
     1681 gtatggctta tactatagcc ctgttagtac cccagcagat tttcggatgc ttgctcctat
     1741 tgtattggaa caagtcttaa aaaaagctgg aacagaattg ttagagccat atcttagttt
     1801 taaaatttat gcgccacagg aatatctttc acgagcatac aacgatgctc ctaaatattg
     1861 tgcgaacatc gtagacactc aattgaaaaa taatgaggtc attcttagtg gagaaatccc
     1921 tgctcggtgt attcaagaat atcgtagtga tttaactttc tttacaaatg gacgtagtgt
     1981 ttgtttaaca gagttaaaag ggtaccatgt tactaccggt gaacctgttt gccagccccg
     2041 tcgtccaaat agtcggatag ataaagtacg atatatgttc aataaaataa cttagtgtat
     2101 tttatgttgt tatataaata tggtttcttg ttaaataaga tgaaatattt tttaataaag
     2161 atttgaatta aagtgtaaag gaggagatag ttattataaa ctacaagtgg atattgtgtc
     2221 ctgtatgtgg aaataaaaca cgattaaaga taagggaaga tactgaatta aaaaaattcc
     2281 ccctctattg tccgaaatgc agacaagaaa atttaattga aataaagcag ttcaaagtaa
     2341 ctgtgattac agagccagac gcaaagacgc agagccgata aaatgagatt aatacaatct
     2401 cattttatcg gctctttccg ttatgtatgg attcttttaa ttagtcttcg atgtttcttg
     2461 cttcgttgat accgctggct aaagattcca ttaaggatag ttctttgtct gtaaagctat
     2521 ccatgtattt ctctatctgt aatcgtcggg tgctttttac caagttatta gcaggtaaga
     2581 aaaattcatc aacggaaaca tgaagtaacg atacaaggtc ataaagaact tgtatgctgg
     2641 ggtgttgccc tttattttca atattagtta agtaccgtgg gtcaatttca atcaatgctc
     2701 ccacttgttc acgagttaaa cctcgtttca atcgagcttc tttaatggct aaaccaaagg
     2761 ctctaaaatc atatttatct tcttttttac gcatagtaga ccacctctat acattttatt
     2821 gttcctactg aattaaaaac aggtatagaa aaacgtgtta tatggtttat aggtttatat
     2881 ttaataaaaa gcactactaa acgccaataa aaaaaaccgt tatatggtag tgctatttac
     2941 gctgttaaaa tattgtatat tacttccaaa tggcggtttg ttggaggtca acgtcgccat
     3001 gaagtacatc atatacaata aatttcctta cattgggttc ttgtcaaaaa aagtcgtcta
     3061 tctgcaatag ataagtacgt ccaccaatgt ggttttataa atcatataga tagaataaca
     3121 gaagcatgta aacagagaaa taaatctgtt tatatgcttt tttggctatt cagaactttt
     3181 ttacaaagtt tatttatcag taatgcaaca aatccccctt tcacattggg actaagagtg
     3241 aaaggagata aacgagcaag gctcacttcc tttcctagac agaaaggggg tgagaaacat
     3301 gaaaccatct tcttttcaga ccacaataga aaatcagttt gactatatct gtaaacgtgc
     3361 tatggaagac gagcgaaaga attatatgct ttatctttca aggattgcaa agcgtgaggt
     3421 gtccttttcg gatgttggcg attatcttgt tagccagttt gcgacaacag ataactattc
     3481 aactgacttt cagattttta cactcaatgg gttatcagta ggcgttgaaa atgatttgtt
     3541 gagtgaagca ttacgtgagt tgccagacaa gaaacgtgaa attctactgc tgttttactt
     3601 tatggacatg agcgattcag aaattgcaga cctgttgaaa ttgaaccgtt ctactgtcta
     3661 tcggcataga accagtggac tagccttaat taaaaagttt atggaggaat ttgaagaatg
     3721 aaaacacaat atcctatgat tccctttcct ctcattgtaa aggcaacaga tggcgatacc
     3781 gaagcgatta accagattct acatcattac agagggtaca taacgaagcg ttccctacga
     3841 cttatgaaag atgaatatgg caatcaaagt atggtcgttg atgaagtctt acgtggaaga
     3901 atggaaacca gactgattac aaagattttg tcatttgaaa ttaagtaata tcctctctcc
     3961 tttcgtggaa gcgtgctaaa ccattccacg cttcccgaac agggaggttt gttattccac
     4021 caaagcatat tgagctttca atgtgttttg ataggctaac gagccattgt tctttgaaaa
     4081 ctgaataaaa gtaatcgaat acgtttcgat aagaaaagag ccaacggaac taaccgccat
     4141 gacctatctt ataaagatag cgagcgattc atgttagtga tccgagaagc aatctttagc
     4201 aggattgcct gcaacgacat tcttatcgtg ataatgatac tcccatacag tcaatagtcc
     4261 gagcgtgata aaaccgtcgc aggcaatgag tatggctaca tgagaaccat gcaggggtgg
     4321 aactcccgtg agctttgcta aagctgttcg attgctggta aaacaacttt tatgaaatcc
     4381 aaataagtga tttggaaagg aggattttat gaagcagact gacattccta tttgggaacg
     4441 ttatacccta accattgaag aagcgtcaaa atattttcgt attggcgaaa acaagctacg
     4501 acgcttggca gaggaaaata aaaatgcaaa ttggctgatt atgaatggca atcgtattca
     4561 gattaaacga aaacaatttg aaaaaattat agatacattg gacgcaatct agcgtcgcca
     4621 aagggtcttg tatatgataa aatagtatta agtcgtatca aggctctttc cataaaggaa
     4681 aggagcaaat gccatgtcag aaaaaagacg tgacaataaa ggtcgaatct taaagactgg
     4741 agagagccaa cgaaaagacg gaagatactt atacaaatat atagattcat ttggagaacc
     4801 gcaatttgtt tactcgtgga aacttgtggc tacagaccga gtaccagcag gaaagcgtga
     4861 ttgtatctca cttagagaga aaatcgcaga gttacagaaa gacattcatg atggtattga
     4921 tgttgtagga aagaaaatga cactctgcca gctttacgca aaacagaacg ctcaaagacc
     4981 aaaggttaga aaaaacactg aaactggacg caaatatctt atggatattt tgaagaaaga
     5041 caagttaggt gtaagaagta ttgacagtat taagccatca gacgctaaag aatgggctat
     5101 tagaatgagt gaaaatggtt atgcttatca aaccatcaat aactacaaac gttctttaaa
     5161 ggcttcattc tatattgcta tacaagatga ttgtgttcgg aagaatccat ttgactttca
     5221 actgaaagca gttcttgatg atgatactgt ccctaaaacc gtactaacag aagaacagga
     5281 agaaaaactg ttagcctttg caaaagctga taaaacctac agcaaaaatt atgatgaaat
     5341 tctgatactc ttaaaaacag gtcttcgtat ttcagagttt ggtggtttga cacttccaga
     5401 tttagatttt gagaatcgtc ttgtcaatat agaccatcag ctattgagag atactgaaat
     5461 tgggtactac attgaaacac caaagaccaa aagtggcgaa cgtcaagttc ctatggttga
     5521 agaagcctat caagcattta agcgagtgtt agcgaatcga aagaatgata agcgtgttga
     5581 gattgatgga tatagtgatt tcctctttct taatagaaag aactatccaa aagtgcaagt
     5641 gattacaacg gcatgatgaa aggtcttgtt aagaaataca ataagtataa cgaggataaa
     5701 ttgccacaca tcactccaca tagtttgcga catacattct gtaccaacta tgcaaatgca
     5761 ggaatgaatc caaaggcatt acagtacatt atgggacatg ctaatatagc catgacgctg
     5821 aactattacg cacatgcaac attcgattct gcaatggcag aaatgaaacg cttgaataaa
     5881 gagaagcaac aggagcgtct tgttgcttag tagtacaaat gaatttacta cttatttacc
     5941 acttctgaca gctaagacat gaggaaatat gcaaagaaac gtgaagtatc ttcctacagt
     6001 aaaaatactc gaaagcacat agaataaggc tttacgagca tttaagaaaa tataaaaaga
     6061 taattagaaa tttatacttt gtttatttca aaattaatcc atgcttaaga cagaccagct
     6121 attattaaag aaaaaataag acatagactg ataggttcca caccaaatgt agccccatac
     6181 tgccccataa gtccgatttg tagcgcacaa gccctaaaaa catcccaagt gaaacataca
     6241 aacaccaagc taaaatggtt cctggatgat gagctaagac aaataaaata cttgtcaaag
     6301 caactcgaat atctaatttt ctaaccaagt tccataaaat ttctcgatac agaaattctt
     6361 caaccatact cgcattgatt aataacaata aaaatgaaaa ccaagggact tgatgttgaa
     6421 ggccaattaa gtttgcttga ttcgtagttc cttgagcatg gattaggcta aaacatagat
     6481 ttataatcag taggctaacg aatccaatac caagccattt catcctagtt ttcatattga
     6541 ccttgacaac ttgttttcgt tgaccataca tccataaaaa agaaatgagc gacgcacaat
     6601 agagaatctg tagtatggtt aactcactaa tacaaagcaa tttcagtaag tatagggata
     6661 ccaataagac atttacttgt tggaatatat aaactggaat tattcttttc atagttacct
     6721 ccgaaataaa tcttcataat ctaaatctaa tacctgcaca atcctttcta cccatggact
     6781 ttgaggcatt cgttgttcca tcttgtagtg gcgtatcttt tgatacaaac gattcaattc
     6841 acttggatag tgaaactctc ccgcaaacat ttttctggtt aactcaatcc agctgatatt
     6901 cctttcagcc aaaataatgg acaagttctc ccaaaatcgt tctgccatat tgattctcct
     6961 ttagttagat aaataatgtg tttgtgccat gtaaatcaat tgtttcgtat ctcttggcaa
     7021 taaagctcta gcctcctcca gattcaaatt tggataaact cgcttatttg aaaccgcaag
     7081 aggaagtctg atggttagtt caggattttt taatatcatt tcgatgaaat ctgttaattt
     7141 taggttatct cgattcttaa aacgtaataa attgggagat aaaaactcga aacaatctga
     7201 agaatagctc atcatctcaa ttaatttatc ctttgtcatt tcagaaatag aatgaggagt
     7261 gacctctatg ccatactttt gaaagaaatc caaaagaagt tgatttctat gacaattttt
     7321 acttagatag agatcaatca tgggagacct cccaaagatt cggttccatt tgatattctg
     7381 agacgattaa ggaatctaat aaatttgaga agttaatcgg tttcttgtct tcatcataag
     7441 cttttacagt tacttgggtt gtaagtattc cctcttttcc ctcggctcga tagccttgtc
     7501 catataaaac aaaaacaaga ttttgatgat catctacaaa ggcatcaacc ccattcttta
     7561 tgtcttgact ttcaaggaat tccatcacgt tttgaagata ggactcgtaa aatagtgggt
     7621 agttgtgttt tttatggtaa tcatctaaaa atgttacttc aaactcacat ggataattgg
     7681 gtatcaaaaa taattgttca tccagctgtt tgatttctgc atcatgtaat tctgtttcta
     7741 attcatcaca atctagtatt gattctttat ttaatgcttt catctttttc ctctatttct
     7801 tttaatttct ttgcgattgc ggcaatcaca ggaacggtta cactattacc agcttgttta
     7861 tagagctgac tattactaga gactcttcta gcagcttcaa aagcccaatc aggaaagccc
     7921 tgcaagcgaa aacactcttt aggagtgatt cgtcgtatcc tcagacggta aaattgtcca
     7981 tctattaata taccggctac atgataaact tgtttatctt ctccttcata actagcaact
     8041 actactccca tttgtccact agttgttaac gtattagcta taccttttcc aactctacca
     8101 cgacgatact gagaacttgg tctttccaaa ttgattgaat ctcctatttc tgcttgagcg
     8161 tatccttttt tcgttgcttc ccggactttt agaaattgaa ttggttctgg aatcagtatt
     8221 ttggggattt tatctccacc ttgcatcgta gtcagtgttg gagataaccc ctcacttcca
     8281 tagacacgac cggtctcctt aaagctagtc ggtaaatctc caacaacgac aatgccatga
     8341 cgatcctgag tatttaaagt aaacattggc tcttggtttt ccttaaagcg tctaccattt
     8401 tgtctcttgt ctaatctatc tggtgtcata caaggaatcg caatcttaaa tccttctcct
     8461 ttcccacgaa ctaaggttgg cgcaagacct tctgaataat aaactttacc gctcattcca
     8521 ctttttgatg gattcaaatt tcctaatgtt tttaaagtct cagagttagt tgcttgacct
     8581 tctcgtctga aaggaaataa gagtctgata cctttctttc tagaatgtcc gataataaac
     8641 accctctctc tgttttgggg aacgccaaaa tccttactgt taagcacctg ccattcaaca
     8701 tcaaacccca actcatcaag tgtggtaagg attgtggtga acgtccgtcc cttatcgtga
     8761 ttgagtagac ctttaacatt ttcaagaaaa agaaaacgtg gttggatttg tttggccgcc
     8821 cgagcaattt caaagaacaa agttcctcta atatcttcaa atcccaatcg tcttcctgcg
     8881 attgaaaatg cttgacaagg aaatccccca cagatgatat cgactttccc tctaagtttt
     8941 ttaaattcgt catctgaaac atctcgtatg tcatgaaatt ctatttctcc ttccgtttga
     9001 aaaatggact tataagattc tctagcaaat ttatcaatct cacaaaatcc aatacactcg
     9061 tgtcctacac tttccattcc aagtcgaaaa ccaccgatac ctgaaaataa atcaataaat
     9121 ctcatttctt gattacccct ttctgatcaa ctatcaatgt ccaaagtgtc acaccacttg
     9181 aaatgacaat gagcataact aatatccact cgattggcga catttaacca cctccttttc
     9241 taacacgatt attccaaaca gaatacaagc cgttaaatac aaattcagca agtacttccg
     9301 ctcttctaac aaagcgaaca ttgtcaagag catactgata gattctctca aaatcagtca
     9361 tagcaatttc actggctgtt tcagaaaaac cttctcgtct aaattgatct tgtaccaatc
     9421 cccaaatata atcttgatca tattttgtga ccttttctac ttttcttttc aagataggtt
     9481 gagtatacct ctcctcctca tcctcactaa ataaagaatc agtctcacta tatttagtct
     9541 cactaacttc agtctcacta ggggctgaat gtgagacggg ggcagtttca ttttcaacct
     9601 gccctatttt ttttttaaca ctaggcctgt ttgaactagc tactgg
//
LOCUS       DAVKGU010000218         5131 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00065451
            SAMN33606079-rid17950993.guided.107, whole genome shotgun sequence.
ACCESSION   DAVKGU010000218 DAVKGU010000000
VERSION     DAVKGU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606079
            Sequence Read Archive: SRR23727485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722527.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722527.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:46:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,995
            CDSs (with protein)               :: 1,995
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 59 of 143
            Pseudo Genes (internal stop)      :: 46 of 143
            Pseudo Genes (multiple problems)  :: 19 of 143
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606079-rid17950993.guided.107"
                     /strain="AUSMDU00065451"
                     /serotype="11A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST166"
     gene            393..707
                     /locus_tag="VW077_002169"
     CDS             393..707
                     /locus_tag="VW077_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU9456090.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            723..1109
                     /locus_tag="VW077_002170"
     CDS             723..1109
                     /locus_tag="VW077_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEU9456091.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1138..2523
                     /locus_tag="VW077_002171"
     CDS             1138..2523
                     /locus_tag="VW077_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9456092.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2526..2678
                     /locus_tag="VW077_002172"
     CDS             2526..2678
                     /locus_tag="VW077_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEU9456093.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2701..3951
                     /locus_tag="VW077_002173"
     CDS             2701..3951
                     /locus_tag="VW077_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEU9456094.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKDICQPYQLTDIQKKI"
     gene            3909..4118
                     /locus_tag="VW077_002174"
     CDS             3909..4118
                     /locus_tag="VW077_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEU9456095.1"
                     /translation="MSTIPVDRYPKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEV
                     MEIRLRSKLKSVLIVHYLKILYNRN"
     gene            4167..4250
                     /locus_tag="VW077_002175"
     CDS             4167..4250
                     /locus_tag="VW077_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEU9456096.1"
                     /translation="MLVFQMHNVDKTSTVLKQTKNSDYADK"
     gene            4375..5112
                     /gene="erm(B)"
                     /locus_tag="VW077_002176"
     CDS             4375..5112
                     /gene="erm(B)"
                     /locus_tag="VW077_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEU9456097.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT         1757 a          905 c         1029 g         1440 t
ORIGIN      
        1 ctatatatgt tagcatttta tttctttaaa atcaattcat ttagtttttt taaaataaaa
       61 atagcataaa aatctagtta tccgcataaa aactggactt atcacacttt atcaaggtca
      121 aaaccactca atttactact aatttactac ttatgaatga gctttgatac gacgatttat
      181 ccttgaaaag tgaagatata aagatacttc caataaaatt tgaatattta ataggtagac
      241 acttcaaaaa atgaggtgtc tattttttta cccgattttg aaaggaagtg aacttatgaa
      301 aacaaaaaat caagaatcaa aaggtcgttc cccactcttt aagaccatca aacattcatt
      361 cagccaataa aaaagaaagg ataggtaaaa atatggaact taaatttgtg attcccaaca
      421 tggaaaaaac attcggcaat ttagaatttg ctggcgagga taaagtcgtt cagcgaagaa
      481 tcaacggacg gctaactgtc ttatcaagaa gctataatct ctattctgat gttcaaagag
      541 cagatgatat tgtggtggtg cttcctgctg aagctggcga aaaacatttc ggctttgagg
      601 aacgtgtgaa gttagtcaat ccacgtatta ccgcagaggg ctacaaaatc ggcactcgtg
      661 gttttacaaa ttacctttta catgctgacg acatgataaa agaataaaga aagagaggaa
      721 aaatgatgag attagcaaat ggcattgtat tagataaaga cacgactttt ggagaattga
      781 aattctctgc tctacgtcgt gaagtgagaa tccaaaatga agacgggtcg gtttcagatg
      841 aaatcaagga acgtacctat gacttaaaat ccaaaggaca aggacgcatg attcaagtaa
      901 gtattcctgc cagcgtgcct ttgaaagagt ttgattataa cgcacgggtg gaacttatca
      961 atcccattgc ggacaccgtt gctactgcca cctatcaagg agcagatgtt gactggtata
     1021 tcaaggcaga cgatattgtg ctgacaaagg attctagttc attcaaagct caaccacaag
     1081 caaagaaaga accgacacaa gacaaatagt cgctaggtag aaaggagact ttttcgcatg
     1141 aaacagcgtg gtaaaaggat tcgcccatct ggtaaagatt tagtctttca ttttacgata
     1201 gcgtcactcc tgcctgtttt cctgctggtt gtcggactgt ttcatgtgaa gacaatccag
     1261 cagatcaact ggcaggattt taacctatca caagcagata agattgacat tccctattta
     1321 attatcagtt tcagtgtcgc aattcttatc tgcttgctgg tagcgtttgt attcaaacgg
     1381 gttcgctatg atacggttaa acaactttac caccgtcaaa aactggcaaa gatgatactt
     1441 gaaaacaagt ggtatgaatc tgaacaggtc aaaacagagg gtttctttaa agatagtgct
     1501 ggtcgtacaa aggaaaagat aacctacttc cctaaaatgt attatcgact taaaaatggc
     1561 ttgatacaga tacgggtgga aatcacgctg ggaaaatatc aagaccaact cttacacttg
     1621 gaaaagaaat tagagagtgg cttgtactgt gagctgacgg ataaagagtt aaaggattcc
     1681 tatgtggaat atactttgct ctatgacacc atagccagtc gtatttctat tgatgaagta
     1741 gaagctaaag atggtaaact tcgcttaatg aaaaacgtat ggtgggaata tgataagctc
     1801 cctcatatgt tgattgctgg tggtacaggt ggcggtaaaa cttactttat actgacactg
     1861 attgaagcct tgcttcatac agattcaaaa ctgtatattc ttgacccgaa aaatgctgac
     1921 cttgcggact taggttctgt gatggcaaat gtctactata gaaaagaaga cttgctttct
     1981 tgcattgaaa cattctatga agaaatgatg aaacgtagtg aggaaatgaa gcagatgaag
     2041 aactataaga ctggcaaaaa ttatgcttac ttaggtctcc cggcacactt cttaatcttt
     2101 gatgaatacg tcgctttcat ggaaatgctg ggaacaaaag aaaacaccgc agttatgaat
     2161 aagctgaaac agattgtcat gttaggtcgt caagctggct tctttctaat actggcttgt
     2221 caacgtccag acgcaaaata tttaggcgac ggaatccgtg atcagtttaa tttcagagtg
     2281 gctttaggtc gtatgtctga aatgggctat ggcatgatgt ttggcagtga cgtacaaaag
     2341 gatttcttct taaagcgaat caaaggtcgt ggctatgttg atgtaggaac aagtgtcata
     2401 tcagagtttt atactcccct tgtaccaaaa ggatatgatt tcttggagga aattaaaaag
     2461 ttatccaaca gcagacagtc cacgcaggcg acgtgcgaag cggaagtcgc aggtgtggac
     2521 tgatcttgct ggctggtgtg gcaatagcca cgccagcact taaccccccg tatctaacag
     2581 gggggtacaa atcgacagga aacagtcaaa aaaacattag aaaatccttt ggttacaagg
     2641 gatttacaaa atttcagcgt atgtcaaatg ggctttaaaa gttgacatac gcctttttga
     2701 ttggagggat ttttactgaa tgaacaaact tggttacagc atttaaaaga aaaacgcttg
     2761 gcttatggac tatctcaaaa ccgtttagct gttgcgactg gtattacaag gcagtatcta
     2821 agcgatattg aaacaggaaa agtcaagcca tcagaggatt tacagcagtc cctttgggaa
     2881 gctctggaac gcttcaatcc cgacgctccc cttgaaatgc tgtttgatta tgtaaggatt
     2941 cgctttccga caacagacgt acagcaggtg gtcgaaaaca tcttacaact gaaactgtcc
     3001 tattttcttc atgaggacta tggtttctat tcttattcag agcattatgc tttaggcgac
     3061 atattcgtcc tttgctccca tgaactggac aaaggagttc tggtggaatt gaaaggtcgt
     3121 gggtgcagac aatttgaaag ctatcttctg gcacaacaaa gaagctggta tgagttcttt
     3181 atggacgttt tggtggctgg cggtgtgatg aaacgccttg accttgccat taacgataag
     3241 acagggattt taaatatccc tgtactcact gaaaagtgcc aacaggaaga atgtatctcc
     3301 gtcttccgca gttttaaaag ctatcgcagt ggcgaactgg tacgcaaaga ggaaaaggaa
     3361 tgtatgggaa acaccctcta tatcggttca ttacaaagtg aagtttattt ctgtatctat
     3421 gaaaaggact acgagcagta caagaaaaat gatattccca ttgaagacgc agaagtaaaa
     3481 aaccgttttg agattcgatt gaaaaatgag cgtgcctatt atgcagtccg tgatttactc
     3541 gtctatgaca atccagagca taccgccttt aaaattatca atcggtatat ccgttttgta
     3601 gataaagacg attccaaacc tcgttctgat tggaaactga atgaagaatg ggcttggttt
     3661 attgggaaca atcgtgaacg attaaaacta accacaaaac cagagcctta ctccttccaa
     3721 aggacgctga actggctatc tcatcaagtt gccccgacct taaaggttgc gattaaactt
     3781 gatgaaatca accagacgca ggttgtaaaa gacattctcg accatgcgaa actgacagac
     3841 cgacacaagc agattttgaa gcaacagtca gtaaaagaac aggacgtgat aacaacaaaa
     3901 aaagatatct gtcaaccata ccagttgaca gatatccaaa aaaagatata atgggagata
     3961 agacggttcg tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg
     4021 gcggaaacgt aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga
     4081 tagtgcatta tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat
     4141 ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca aatgcataat gtagataaaa
     4201 catctactgt tttgaaacag actaaaaaca gtgattacgc agataaataa atacgttaga
     4261 ttaattccta ccagtgacta atcttatgac tttttaaaca gataactaaa attacaaaca
     4321 aatcgtttaa cttctgtatt tgtttataga tgtaatcact tcaggagaga ttacatgaac
     4381 aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa ccaaataata
     4441 aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg taaagggcat
     4501 ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt agacagtcat
     4561 ctattcaact tatcgtcaga aaaattaaaa ctgaatactc gtgtcacttt aattcaccaa
     4621 gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg gaatattcct
     4681 taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccgtgc gtctgacatc
     4741 tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg aacactaggg
     4801 ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc ggaatgcttt
     4861 catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac cacagatgtt
     4921 ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa tcgagaatat
     4981 cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa agtaaacaat
     5041 ttaagtacca ttacttatga gcaagtattg tctattttta atagttatct attatttaac
     5101 gggaggaaat aattctatga gtcgcttttt t
//
